LOCUS       DAJTSF010000001       136769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000001 DAJTSF010000000
VERSION     DAJTSF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 136769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 136769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..136769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.001"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OO335_000001"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OO335_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR6000559.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OO335_000002"
     CDS             complement(1672..2730)
                     /locus_tag="OO335_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6000560.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OO335_000003"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OO335_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OO335_000004"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OO335_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR6000561.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO335_000005"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO335_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6000562.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO335_000006"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO335_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6000563.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OO335_000007"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OO335_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6000564.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OO335_000008"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OO335_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6000565.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO335_000009"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO335_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6000566.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO335_000010"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO335_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6000567.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO335_000011"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO335_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6000568.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO335_000012"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO335_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6000569.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OO335_000013"
     CDS             complement(17699..18322)
                     /locus_tag="OO335_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6000570.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="OO335_000014"
     CDS             18619..18894
                     /locus_tag="OO335_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000571.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OO335_000015"
     CDS             complement(18971..19213)
                     /locus_tag="OO335_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6000572.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OO335_000016"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OO335_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6000573.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="OO335_000017"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="OO335_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6000574.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO335_000018"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO335_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6000575.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="OO335_000019"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="OO335_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24093..24827
                     /gene="ypdB"
                     /locus_tag="OO335_000020"
     CDS             24093..24827
                     /gene="ypdB"
                     /locus_tag="OO335_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR6000576.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="OO335_000021"
                     /pseudo
     CDS             24840..25697
                     /locus_tag="OO335_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OO335_000022"
     CDS             complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OO335_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6000577.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="OO335_000023"
     CDS             complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="OO335_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6000578.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="OO335_000024"
                     /pseudo
     CDS             complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="OO335_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30355..31602)
                     /locus_tag="OO335_000025"
     CDS             complement(30355..31602)
                     /locus_tag="OO335_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6000579.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31624..31950)
                     /locus_tag="OO335_000026"
     CDS             complement(31624..31950)
                     /locus_tag="OO335_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6000580.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32169..33134)
                     /gene="glk"
                     /locus_tag="OO335_000027"
     CDS             complement(32169..33134)
                     /gene="glk"
                     /locus_tag="OO335_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6000581.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33338..34594
                     /locus_tag="OO335_000028"
     CDS             33338..34594
                     /locus_tag="OO335_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6000582.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34709..35035
                     /locus_tag="OO335_000029"
     CDS             34709..35035
                     /locus_tag="OO335_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6000583.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35176..36414)
                     /locus_tag="OO335_000030"
     CDS             complement(35176..36414)
                     /locus_tag="OO335_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6000584.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36750..37952
                     /gene="nupC"
                     /locus_tag="OO335_000031"
     CDS             36750..37952
                     /gene="nupC"
                     /locus_tag="OO335_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6000585.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38002..40114)
                     /locus_tag="OO335_000032"
                     /pseudo
     CDS             complement(38002..40114)
                     /locus_tag="OO335_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40322..40397)
                     /locus_tag="OO335_000033"
     tRNA            complement(40322..40397)
                     /locus_tag="OO335_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40362..40364),aa:Ala,seq:ggc)
     gene            complement(40437..40512)
                     /locus_tag="OO335_000034"
     tRNA            complement(40437..40512)
                     /locus_tag="OO335_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40477..40479),aa:Ala,seq:ggc)
     gene            40733..41092
                     /locus_tag="OO335_000035"
     CDS             40733..41092
                     /locus_tag="OO335_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6000586.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41094..41486
                     /locus_tag="OO335_000036"
     CDS             41094..41486
                     /locus_tag="OO335_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6000587.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41538..42953)
                     /gene="gltX"
                     /locus_tag="OO335_000037"
     CDS             complement(41538..42953)
                     /gene="gltX"
                     /locus_tag="OO335_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6000588.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43212..43287
                     /locus_tag="OO335_000038"
     tRNA            43212..43287
                     /locus_tag="OO335_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43245..43247,aa:Val,seq:tac)
     gene            43332..43407
                     /locus_tag="OO335_000039"
     tRNA            43332..43407
                     /locus_tag="OO335_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43365..43367,aa:Val,seq:tac)
     gene            43412..43487
                     /locus_tag="OO335_000040"
     tRNA            43412..43487
                     /locus_tag="OO335_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43445..43447,aa:Lys,seq:ttt)
     gene            43518..43643
                     /locus_tag="OO335_000041"
     ncRNA           43518..43643
                     /ncRNA_class="other"
                     /locus_tag="OO335_000041"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43763..44104
                     /locus_tag="OO335_000042"
     CDS             43763..44104
                     /locus_tag="OO335_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6000589.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44095..45021)
                     /locus_tag="OO335_000043"
     CDS             complement(44095..45021)
                     /locus_tag="OO335_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6000590.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45111..46109
                     /locus_tag="OO335_000044"
     CDS             45111..46109
                     /locus_tag="OO335_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6000591.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46106..46324)
                     /locus_tag="OO335_000045"
     CDS             complement(46106..46324)
                     /locus_tag="OO335_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6000592.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46326..48341)
                     /gene="ligA"
                     /locus_tag="OO335_000046"
     CDS             complement(46326..48341)
                     /gene="ligA"
                     /locus_tag="OO335_000046"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6000593.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48412..49398)
                     /gene="zipA"
                     /locus_tag="OO335_000047"
     CDS             complement(48412..49398)
                     /gene="zipA"
                     /locus_tag="OO335_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6000594.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49628..50389
                     /gene="cysZ"
                     /locus_tag="OO335_000048"
     CDS             49628..50389
                     /gene="cysZ"
                     /locus_tag="OO335_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6000595.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50574..51545
                     /gene="cysK"
                     /locus_tag="OO335_000049"
     CDS             50574..51545
                     /gene="cysK"
                     /locus_tag="OO335_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6000596.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51929..52186
                     /gene="ptsH"
                     /locus_tag="OO335_000050"
     CDS             51929..52186
                     /gene="ptsH"
                     /locus_tag="OO335_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6000597.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52231..53958
                     /gene="ptsI"
                     /locus_tag="OO335_000051"
     CDS             52231..53958
                     /gene="ptsI"
                     /locus_tag="OO335_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6000598.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53999..54508
                     /gene="crr"
                     /locus_tag="OO335_000052"
     CDS             53999..54508
                     /gene="crr"
                     /locus_tag="OO335_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6000599.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54550..55401)
                     /gene="pdxK"
                     /locus_tag="OO335_000053"
                     /gene_synonym="thiJ"
     CDS             complement(54550..55401)
                     /gene="pdxK"
                     /locus_tag="OO335_000053"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6000600.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55506..55880
                     /locus_tag="OO335_000054"
     CDS             55506..55880
                     /locus_tag="OO335_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6000601.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55913..56636
                     /locus_tag="OO335_000055"
                     /pseudo
     CDS             55913..56636
                     /locus_tag="OO335_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56840..57751)
                     /gene="cysM"
                     /locus_tag="OO335_000056"
     CDS             complement(56840..57751)
                     /gene="cysM"
                     /locus_tag="OO335_000056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6000602.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57885..58982)
                     /gene="cysA"
                     /locus_tag="OO335_000057"
     CDS             complement(57885..58982)
                     /gene="cysA"
                     /locus_tag="OO335_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6000603.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58972..59847)
                     /gene="cysW"
                     /locus_tag="OO335_000058"
     CDS             complement(58972..59847)
                     /gene="cysW"
                     /locus_tag="OO335_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6000604.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59847..60680)
                     /gene="cysT"
                     /locus_tag="OO335_000059"
     CDS             complement(59847..60680)
                     /gene="cysT"
                     /locus_tag="OO335_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6000605.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60680..61696)
                     /gene="cysP"
                     /locus_tag="OO335_000060"
     CDS             complement(60680..61696)
                     /gene="cysP"
                     /locus_tag="OO335_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6000606.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(62000..62791)
                     /gene="ucpA"
                     /locus_tag="OO335_000061"
     CDS             complement(62000..62791)
                     /gene="ucpA"
                     /locus_tag="OO335_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6000607.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62978..63778)
                     /gene="murR"
                     /locus_tag="OO335_000062"
     CDS             complement(62978..63778)
                     /gene="murR"
                     /locus_tag="OO335_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6000608.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63942..64838
                     /gene="murQ"
                     /locus_tag="OO335_000063"
     CDS             63942..64838
                     /gene="murQ"
                     /locus_tag="OO335_000063"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6000609.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64842..66266
                     /gene="murP"
                     /locus_tag="OO335_000064"
     CDS             64842..66266
                     /gene="murP"
                     /locus_tag="OO335_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR6000610.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66446..67345)
                     /gene="yfeX"
                     /locus_tag="OO335_000065"
     CDS             complement(66446..67345)
                     /gene="yfeX"
                     /locus_tag="OO335_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6000611.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67441..68016)
                     /locus_tag="OO335_000066"
     CDS             complement(67441..68016)
                     /locus_tag="OO335_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6000612.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68077..68526)
                     /locus_tag="OO335_000067"
     CDS             complement(68077..68526)
                     /locus_tag="OO335_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6000613.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68513..68938)
                     /locus_tag="OO335_000068"
     CDS             complement(68513..68938)
                     /locus_tag="OO335_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6000614.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69152..70021
                     /gene="amiA"
                     /locus_tag="OO335_000069"
     CDS             69152..70021
                     /gene="amiA"
                     /locus_tag="OO335_000069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6000615.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70025..70924
                     /gene="hemF"
                     /locus_tag="OO335_000070"
     CDS             70025..70924
                     /gene="hemF"
                     /locus_tag="OO335_000070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6000616.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70930..71982)
                     /gene="eutR"
                     /locus_tag="OO335_000071"
                     /pseudo
     CDS             complement(70930..71982)
                     /gene="eutR"
                     /locus_tag="OO335_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(72028..72528)
                     /gene="eutK"
                     /locus_tag="OO335_000072"
     CDS             complement(72028..72528)
                     /gene="eutK"
                     /locus_tag="OO335_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6000617.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72541..73200)
                     /gene="eutL"
                     /locus_tag="OO335_000073"
     CDS             complement(72541..73200)
                     /gene="eutL"
                     /locus_tag="OO335_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6000618.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74382..75173
                     /locus_tag="OO335_000074"
     CDS             74382..75173
                     /locus_tag="OO335_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000619.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75893..76288
                     /locus_tag="OO335_000075"
     CDS             75893..76288
                     /locus_tag="OO335_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6000620.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76306..76569
                     /locus_tag="OO335_000076"
     CDS             76306..76569
                     /locus_tag="OO335_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000621.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76592..77134
                     /locus_tag="OO335_000077"
     CDS             76592..77134
                     /locus_tag="OO335_000077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000622.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77273..77560
                     /locus_tag="OO335_000078"
     CDS             77273..77560
                     /locus_tag="OO335_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000623.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77599..77805
                     /locus_tag="OO335_000079"
     CDS             77599..77805
                     /locus_tag="OO335_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000624.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78117..78602
                     /locus_tag="OO335_000080"
     CDS             78117..78602
                     /locus_tag="OO335_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6000625.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78583..78861
                     /locus_tag="OO335_000081"
     CDS             78583..78861
                     /locus_tag="OO335_000081"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000626.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78854..80314
                     /locus_tag="OO335_000082"
     CDS             78854..80314
                     /locus_tag="OO335_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6000627.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80348..81157
                     /gene="murI"
                     /locus_tag="OO335_000083"
     CDS             80348..81157
                     /gene="murI"
                     /locus_tag="OO335_000083"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6000628.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81276..81970)
                     /locus_tag="OO335_000084"
     CDS             complement(join(81276..81724,81724..81970))
                     /locus_tag="OO335_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6000629.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82489..83862)
                     /gene="eutA"
                     /locus_tag="OO335_000085"
     CDS             complement(82489..83862)
                     /gene="eutA"
                     /locus_tag="OO335_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6000630.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83837..84316)
                     /gene="eutP"
                     /locus_tag="OO335_000086"
     CDS             complement(83837..84316)
                     /gene="eutP"
                     /locus_tag="OO335_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6000631.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(84329..84664)
                     /gene="eutS"
                     /locus_tag="OO335_000087"
     CDS             complement(84329..84664)
                     /gene="eutS"
                     /locus_tag="OO335_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6000632.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(84957..87236)
                     /gene="maeB"
                     /locus_tag="OO335_000088"
     CDS             complement(84957..87236)
                     /gene="maeB"
                     /locus_tag="OO335_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6000633.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            87525..88475
                     /gene="tal"
                     /locus_tag="OO335_000089"
     CDS             87525..88475
                     /gene="tal"
                     /locus_tag="OO335_000089"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6000634.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            88495..90498
                     /gene="tkt"
                     /locus_tag="OO335_000090"
     CDS             88495..90498
                     /gene="tkt"
                     /locus_tag="OO335_000090"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6000635.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(90601..91644)
                     /locus_tag="OO335_000091"
     CDS             complement(90601..91644)
                     /locus_tag="OO335_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6000636.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(91770..92345)
                     /gene="nudK"
                     /locus_tag="OO335_000092"
     CDS             complement(91770..92345)
                     /gene="nudK"
                     /locus_tag="OO335_000092"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6000637.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(92413..94392)
                     /gene="aegA"
                     /locus_tag="OO335_000093"
     CDS             complement(92413..94392)
                     /gene="aegA"
                     /locus_tag="OO335_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR6000638.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            94598..96315
                     /gene="narQ"
                     /locus_tag="OO335_000094"
                     /pseudo
     CDS             94598..96315
                     /gene="narQ"
                     /locus_tag="OO335_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            96517..99630
                     /gene="acrD"
                     /locus_tag="OO335_000095"
     CDS             96517..99630
                     /gene="acrD"
                     /locus_tag="OO335_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR6000639.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(99729..99788)
                     /gene="ypfM"
                     /locus_tag="OO335_000096"
     CDS             complement(99729..99788)
                     /gene="ypfM"
                     /locus_tag="OO335_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6000640.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            100169..100525
                     /locus_tag="OO335_000097"
     CDS             100169..100525
                     /locus_tag="OO335_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6000641.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            100529..101656
                     /gene="dapE"
                     /locus_tag="OO335_000098"
     CDS             100529..101656
                     /gene="dapE"
                     /locus_tag="OO335_000098"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6000642.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            101684..101884
                     /locus_tag="OO335_000099"
     CDS             101684..101884
                     /locus_tag="OO335_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6000643.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(101994..102692)
                     /gene="ypfH"
                     /locus_tag="OO335_000100"
     CDS             complement(101994..102692)
                     /gene="ypfH"
                     /locus_tag="OO335_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6000644.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(102766..104781)
                     /gene="tmcA"
                     /locus_tag="OO335_000101"
     CDS             complement(102766..104781)
                     /gene="tmcA"
                     /locus_tag="OO335_000101"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6000645.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(104796..105659)
                     /locus_tag="OO335_000102"
     CDS             complement(104796..105659)
                     /locus_tag="OO335_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6000646.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(105827..106540)
                     /gene="purC"
                     /locus_tag="OO335_000103"
     CDS             complement(105827..106540)
                     /gene="purC"
                     /locus_tag="OO335_000103"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6000647.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(106753..107787)
                     /gene="bamC"
                     /locus_tag="OO335_000104"
     CDS             complement(106753..107787)
                     /gene="bamC"
                     /locus_tag="OO335_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6000648.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(107804..108682)
                     /gene="dapA"
                     /locus_tag="OO335_000105"
     CDS             complement(107804..108682)
                     /gene="dapA"
                     /locus_tag="OO335_000105"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6000649.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            108828..109400
                     /locus_tag="OO335_000106"
     CDS             108828..109400
                     /locus_tag="OO335_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6000650.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            109400..109870
                     /gene="bcp"
                     /locus_tag="OO335_000107"
     CDS             109400..109870
                     /gene="bcp"
                     /locus_tag="OO335_000107"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6000651.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            110123..110740
                     /gene="hyfA"
                     /locus_tag="OO335_000108"
     CDS             110123..110740
                     /gene="hyfA"
                     /locus_tag="OO335_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6000652.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            110740..112757
                     /gene="hyfB"
                     /locus_tag="OO335_000109"
                     /pseudo
     CDS             110740..112757
                     /gene="hyfB"
                     /locus_tag="OO335_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            112768..113715
                     /gene="hyfC"
                     /locus_tag="OO335_000110"
     CDS             112768..113715
                     /gene="hyfC"
                     /locus_tag="OO335_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6000653.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            113732..115171
                     /gene="hyfD"
                     /locus_tag="OO335_000111"
     CDS             113732..115171
                     /gene="hyfD"
                     /locus_tag="OO335_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR6000654.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            115183..115833
                     /gene="hyfE"
                     /locus_tag="OO335_000112"
     CDS             115183..115833
                     /gene="hyfE"
                     /locus_tag="OO335_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6000655.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            115838..117424
                     /gene="hyfF"
                     /locus_tag="OO335_000113"
     CDS             115838..117424
                     /gene="hyfF"
                     /locus_tag="OO335_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6000656.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            117414..119128
                     /gene="hyfG"
                     /locus_tag="OO335_000114"
                     /pseudo
     CDS             117414..119128
                     /gene="hyfG"
                     /locus_tag="OO335_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            119138..119683
                     /gene="hyfH"
                     /locus_tag="OO335_000115"
     CDS             119138..119683
                     /gene="hyfH"
                     /locus_tag="OO335_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6000657.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            119680..120438
                     /gene="hyfI"
                     /locus_tag="OO335_000116"
     CDS             119680..120438
                     /gene="hyfI"
                     /locus_tag="OO335_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6000658.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            120431..120844
                     /gene="hyfJ"
                     /locus_tag="OO335_000117"
     CDS             120431..120844
                     /gene="hyfJ"
                     /locus_tag="OO335_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6000659.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            120874..122703
                     /gene="hyfR"
                     /locus_tag="OO335_000118"
     CDS             120874..122703
                     /gene="hyfR"
                     /locus_tag="OO335_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR6000660.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            122704..122886
                     /locus_tag="OO335_000119"
     CDS             122704..122886
                     /locus_tag="OO335_000119"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR6000661.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            122908..123756
                     /locus_tag="OO335_000120"
                     /pseudo
     CDS             122908..123756
                     /locus_tag="OO335_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(123794..124855)
                     /locus_tag="OO335_000121"
     CDS             complement(123794..124855)
                     /locus_tag="OO335_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6000662.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            125068..126531
                     /gene="bepA"
                     /locus_tag="OO335_000122"
     CDS             125068..126531
                     /gene="bepA"
                     /locus_tag="OO335_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6000663.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            126552..126911
                     /gene="arsC"
                     /locus_tag="OO335_000123"
     CDS             126552..126911
                     /gene="arsC"
                     /locus_tag="OO335_000123"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6000664.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(127044..127790)
                     /gene="hda"
                     /locus_tag="OO335_000124"
     CDS             complement(127044..127790)
                     /gene="hda"
                     /locus_tag="OO335_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6000665.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(127840..129129)
                     /gene="uraA"
                     /locus_tag="OO335_000125"
     CDS             complement(127840..129129)
                     /gene="uraA"
                     /locus_tag="OO335_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6000666.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(129215..129841)
                     /gene="upp"
                     /locus_tag="OO335_000126"
     CDS             complement(129215..129841)
                     /gene="upp"
                     /locus_tag="OO335_000126"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6000667.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            130165..131202
                     /gene="purM"
                     /locus_tag="OO335_000127"
     CDS             130165..131202
                     /gene="purM"
                     /locus_tag="OO335_000127"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6000668.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            131202..131840
                     /gene="purN"
                     /locus_tag="OO335_000128"
     CDS             131202..131840
                     /gene="purN"
                     /locus_tag="OO335_000128"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6000669.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            132012..134078
                     /gene="ppk1"
                     /locus_tag="OO335_000129"
     CDS             132012..134078
                     /gene="ppk1"
                     /locus_tag="OO335_000129"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6000670.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            134083..135624
                     /gene="ppx"
                     /locus_tag="OO335_000130"
     CDS             134083..135624
                     /gene="ppx"
                     /locus_tag="OO335_000130"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6000671.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(135663..>136769)
                     /gene="pdeF"
                     /locus_tag="OO335_000131"
     CDS             complement(135663..>136769)
                     /gene="pdeF"
                     /locus_tag="OO335_000131"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6000672.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        34146 a        35186 c        33600 g        33837 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattaattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttt aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 attcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttt aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
    25861 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
    25921 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
    25981 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
    26041 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
    26101 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
    26161 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
    26221 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
    26281 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
    26341 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
    26401 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
    26461 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
    26521 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
    26581 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
    26641 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
    26701 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
    26761 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
    26821 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
    26881 taatcggctt gtcgcccgcc gccagtagca cctgctggta ggcttcaaat tgctcctgct
    26941 cgtccggcgc gctgtcgcga tccatataga gcatttcggt acggaacagc ccgacacctt
    27001 ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt gccgatattc gccgcaatat
    27061 caatacgctt gttatcacgg gaataggcca gctgcgcggc agcctgcgcc tgttgttttt
    27121 ggcgtttatc cgccagcgtc tgcgcgacct gataataacc gctcaccgcg tcattcgggt
    27181 taatcgccag cacgccgcac tgggcgtcaa gcactgcagg ttgcccggca taacgggcaa
    27241 tcgcatccag cggcaagcca ctcagtaccg ggatcgccga ggcacgggcc agaatcagtg
    27301 tatgcgaggt gcgcccggtt ttctccagaa tcatacccgc aagatttttc aaatcaaggc
    27361 tcaaaaactg gcttggggtt aaatcttcag ccaccagaat ggtcggtttt tccagcacca
    27421 gattgttgcg cggcttcagt tccggccagg tgatatgcag caactgttcg ctgatatcgc
    27481 gaatgtcgct gacccgctcg cgcagataat cgctggcaga agcagatagt ttggcgcaaa
    27541 cctgctccat attgctgatg atcgccgccc ccagtccctg atgctgttct gtcatcaggc
    27601 gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgagcg ctgaggatag
    27661 ttttgctttc gccgtcacgc tcacgcaatt gctgattcag ttgctcggca agcgttgcca
    27721 ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gattgcccga taactgtcga
    27781 ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac gccgcttgcc agcacattgc
    27841 cgtacagtaa atccgggtta aggcggctta atgaacgcgg cagcggatgc gccgtcagtt
    27901 ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg caccaggatg tactcttcca
    27961 gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa ctgtcattaa
    28021 ataaggtgcc agttccaatc aacgccagcg agcttttagc atcagccttt gcgttctggc
    28081 gatggttgat aaaggtgatt tcgctttgcc actggctgca ctgttcttta agttcccacg
    28141 ccggacgagc atgtagaccg ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg
    28201 ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc tcacgcgtaa
    28261 gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg caatcggcaa
    28321 tcgatgccgc gcaatgtccg tggcgggtgg caggccccat taccaatgtg ggtacgccag
    28381 taccggttaa gtgcaccgct ccaccgtccg tgccgccgtt actgaacata tccgcctgca
    28441 acggaacgcc aatttctgcc gccacggttt caatccacgc ggtaagtttt ggcggcgcaa
    28501 tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga tggttagccg
    28561 cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg acgtccggcg
    28621 acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac ctcttcgctg gaacttgcca
    28681 ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgtagtaac gtcaccagca
    28741 gatagcaact gaggcgatca tcaaaggctt tgcccatcac ccgctggtga gggaggacct
    28801 gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat tcccgcctgc atcacttcgt
    28861 cataggtgcg cgcgccaatg tccacgcgca tggcgctaac gtcattcccc tgccggtcgc
    28921 cgtcaagcag gcctggaatt ttgcactctt cacgggtggt gatgcgcacc ggctgcagct
    28981 ggcgggcagc catgcgcacg ttgccaaccg gcagcacgtc aatagcgcct tcgcgggaga
    29041 tgctgcgcac cataaatccc acttcgtcca tatgggcgca gatcatcacc ttcggacctg
    29101 tcgattcatt gaggcggatc agcaccgatc ccagaccatc aaatcgcact tctttttgca
    29161 ggcgagccgc ttcttccagc aggatctgcc gcacttcctg ttccgaggag gcgatcgcat
    29221 ctgcctcgct caacgctttt aatagcgata aatccattat gcctctcccg tgagcaacac
    29281 tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtaaccagca caacatcttc
    29341 gatgcgcacg cccccttgcc ctagcaaata aatccccggc tccacggtca gcaacatgcc
    29401 tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga tcttcatgga cttcaatgcc
    29461 gatagcgtga ccagtgttat gaccgaaata gtcgccaaat ccagcctcgg taatgacccg
    29521 gcgcgcggcg tcgtcaatct gctggcagcg cacgccgggg cgaattgcag agattgccgc
    29581 gagctgtgcc tgcaggacaa tctgatagac gtcaaacagc gggtgagatt cggcgctcac
    29641 cccttcgcca ttcaccagca aggtgcgcgt catatcagca gtagccctga tacagcgcac
    29701 caaaatcgag agtgacaaac tcgcccgctg caacaatctt gtcgctggct ttgccgtgcg
    29761 gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa agaggctttt tctgcgccct
    29821 gctggcgcat aaaccactcc agttcagcgg ctatctcgcg ctcgctcatc cccgcctgaa
    29881 taaagcggcg aatatgctcc gctccgcgat cggcaatccc acaggcgagg cggattatct
    29941 ccacctcctc tggcgttttg atttgccgca gcacatccgg cgtggcgcta accagtttcg
    30001 cattgagttc agacttccag cggtgcgcgg tttcccaact cacctgctgg ccttcaaaac
    30061 cgagcgtttg caactgctca tcggcaatga tttgattgac gatagtggta agcgtgttcg
    30121 ttgcgtcaag caaatgcagc tggtagcctt gcgcacgggc ttccacctca acgaaatagc
    30181 gcgaatctac cagaatgtgc gcactttcac ggctaataac cacatagcct gatccggtgg
    30241 agatccccag atgcggctgt ttgttctgcc gtgaggagag aagcaccgca tccagttgtt
    30301 gcaccttaag ccagttgcgc agcgaagcga gtaatgtcat ctggattttc cttcttacag
    30361 gctatcgatt aacaatttgc ctttgcggaa catcatcaga cgcaggaaca ccaccatcag
    30421 cgcagtaatg attgccccca gcgcgatccc cgccatataa acgcccaagt tagtgaccag
    30481 cggccatgcc cagatagcgg attccgggaa ccattgcact gcgcccagcc agacagctgc
    30541 ggttgagccg acaatcgcgc cgaccatata tgacggaata gcggtgatgg ggctttccag
    30601 cgcaaacgga atcgcaccct cactaatccc cataaaggcc aggaacatcg cggtcttacc
    30661 ctgcggataa agttgagcgt tgaacaggcg tttgccggtt aaacgacggt caataatggt
    30721 cgccagaccc agaccaatcg gcggaataac gatagcgata gaacgagcgg tgaccggtaa
    30781 tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg gctttgttga tcgggccacc
    30841 aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc ccttctcacc
    30901 tgcggcagtc agcacagtac ggataccgcc gttgatccag ccaccaaagg gagtgatgac
    30961 gtagtacatc gccagcatga caaaaatggc ggaaagaatc ggtagcaaaa atgtggtttt
    31021 gaacgctaac aggaaatccg gtaactggat tttctggttc atccatttca caaggtaacc
    31081 ggcaacgata gaaataatca gcgcaccaat gaaggtggac ggcaccggcg aagaggtcgc
    31141 ccattgcata gtgctgggat cgaagttcag cagttgcgtc ggctgggtag acatcaaccc
    31201 gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca caaatgcagc
    31261 aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca gccaggcaaa
    31321 tttaagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat ccatgatgcc
    31381 gatctcagca ggaattttaa gccagctata agcaatcagc tggctgaaag cgaggatcac
    31441 accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat gctgcgggag
    31501 ttcaccccag aagctgcttt tagaggcctg cggattctta actgccgctg ccgcaccgga
    31561 tgcgccaggc acaacggttg cactgcgttt tttaatggcc ataacaatta tccctgtcga
    31621 atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgatgc ccgcagcgtt
    31681 tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac gttcgttatc
    31741 ttccggggta actgccacgg aatggatgat gatggtcgct tcggcgatat cctgcgccgt
    31801 caggcgattc tggatgccgt ccgcgccctg ggtttcaatt ttcacttcat aaccggcttc
    31861 taccgccgct tcttccagca cctgagcggc cataaaagtg tgagccaggc ccattgggca
    31921 ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg atagttgact
    31981 agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag acaaataatg catttactgg
    32041 agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg gacacaaaga
    32101 aaaattgaat taactgtatg atttaaaaga ttatcgggag agttacctcc cgatataaca
    32161 ggaaggattt acagaatgtg acctaaggtc tggcgtaaat gtgcaccgga accgagaaga
    32221 cccggatagt catggacgat gagatacacc ggaatatcat ggacatattc tttaaagcgc
    32281 cctttatctt caaatgcggc acggaaaccg gaggctttga agaactcaag gaagcgcggc
    32341 acgataccgc ccgcaataaa cacgccgcca aatgtcccga gattgagcgc cagattgccg
    32401 ccaaaacggc ccataatgac gcaaaacagc gacaatgcgc ggcggcaatc ggtgcagctg
    32461 tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag gcggttgtca
    32521 gctttcacaa ttgcgcgata caaattcacc agcccagggc cagaaagcac gcgctccgcc
    32581 gaaacatgac caatttccgc acgcaatatt tcgaggataa tggcctcttc ttcactattc
    32641 ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta cccaacgctt atcgacatgg
    32701 accagatgcg caaccccaag ccccgttccg gcaccgtaaa ccgcaatagg cttaccttcg
    32761 accggttctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg gatcgccatc
    32821 gatacagcgg taaaatcgtt aataatttcc agatggctaa aaccgagatt ctttttcatt
    32881 tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc ggtaattggg
    32941 caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag ataaacgcga
    33001 atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg cgagatttca
    33061 ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc accgactaat
    33121 gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt ctcacactgt
    33181 aaatacctgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa tatcgatctg
    33241 ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt tgcaagctgg
    33301 gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc gagccagaac
    33361 catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc ttattctgat
    33421 tgtggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac tgccgggaac
    33481 tctggggata gcccaggatt cacccctctg gatcatcggt gtattaacgc taacgggtat
    33541 tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagacc ccgcctgtga
    33601 accgctgatc ggcgcaccgg ttccgccctc tgcgctacct agacttatcg tagcattaat
    33661 tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcatga ccgtcaatat
    33721 cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc gtcaaccgaa tggagtggac
    33781 tattttagcc tctgccggaa ccatcggcgc gctgtttggt acacctgttg cggcggcgtt
    33841 gatattttcg caaaccttaa atggcagtag tgaagttccg ctatgggatc gtctttttgc
    33901 gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc atccccattt
    33961 ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca gcggtgcaat
    34021 tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac cacggttgca
    34081 cgcgatgatg aatcaaatga aaaatccggt gctcgtgctg ggtattggcg gatttattct
    34141 cggaattctg ggggttattg gtggaccagt ttcgctgttt aaagggctgg atgagatgca
    34201 gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg cggtaattaa
    34261 acttgccgcc ctggtcgttg ctgccgccag tggctttcgc ggtgggcgaa tcttcccggc
    34321 agtgtttgtc ggcgtggcat tagggttgat gctgcatgag cacgttcccg ccgttccagc
    34381 ggcaataacc gtttcctgcg ctattctcgg catcgtgctg gtggtaacac gcgatggctg
    34441 gttaagtctt tttatggcgg cagtcgttgt acccaatacc acattgctac cgctgctctg
    34501 tatcgtcatg cttccggcat ggctgttatt agcaggtaag ccgatgatga tggtcaatcg
    34561 tccgaagcaa cagccacccc acgataatgt ttagcaaaaa atgctctcct ttatgattta
    34621 agagttatgg cgatgatacg gggctttacc tccccgtaat attgcgttaa caggccgctg
    34681 acggcttcga tcgtgaagga gaataacaat gttcaggtca ctgtttctgg cggccgccct
    34741 gatggcattt accccgcttg cagcaaatgc aggtgaaatc accctactgc catcgatcaa
    34801 attacaaatt ggcgatcgcg atcattacgg taattactgg gacggtggtc actggcgaga
    34861 tcgtgattac tggcatcgca attatgaatg gcgcaaaaac cgctggtggc gtcatgataa
    34921 tggctatcat cgtggctggg ataagcgtaa agcatatgag cgtggctatc gtgaaggctg
    34981 gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat gggcaccgcc attaaacgcg
    35041 tcctccaatg gagcacaatg cctgatgcga cgctagcgcg tcttatcagg cctacaaacc
    35101 ggctctttaa cgcgtagggc ggataaggcg tttacgccgc atccgccaat agtgccagat
    35161 gcgacgctca ttcaactaca atcccagcgc cgtccccacc aacaaccaga tattcagcgc
    35221 cacgaccagc actacaatca cccagcctgt ctgttttacg cgtttgctgt tcaccagatc
    35281 gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga accagcgcca gggcgatacc
    35341 aaaacttaac agcacctgac tcataaccag aatccgtgtc ggatctaatc ccatcagaat
    35401 gacaataaat gacggcaaca tggtgactgt acgacgcacc cacagcggga tatggaagcg
    35461 aatgaatccc tgcatcacca cctgccccgc cagtgtcccc accaccgttg aggacagtcc
    35521 ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca gcggttgcag
    35581 cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt ggaacgccgc
    35641 cgcagctgta gccatcatca ccagattgac aaaaccggca atcgtcatgg cgatagccac
    35701 atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat gctgagtgag
    35761 cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc ctgctgccag
    35821 gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt cctttaccca gctgcgccag
    35881 gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca acagtaaccc
    35941 gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa tcaggaaagt
    36001 cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga gtttaaaacc
    36061 gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt ctgcctgaac
    36121 ccaatagaac cacactacgg gacgcggata gtgatcacga atctgctccg ccagattttt
    36181 accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca tcaggttggc
    36241 ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa tattggtcgc
    36301 aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca ttaatgcgag
    36361 cctcatctta cgcgccgccc gtccgctgct actctcaacg cgatagttcg tcatcttgtg
    36421 cctctaaaac atagcctttg ctatgtttca tgctatgcta aacgagaatg attatcaaat
    36481 tcatttaaat ggattgtggt gatttctctg atagaccaag attatgatta cgaaaagatt
    36541 gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact ttcagcttcc
    36601 atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt cacaggacgt
    36661 cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat gtgcacggaa
    36721 atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt gtactggcac
    36781 ttgccgttgt tgtgattctc gcactgctgg taagcagcga ccgcaaaaaa attcgtatcc
    36841 gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc ctgaactccg
    36901 acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg ctcggatttg
    36961 ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg gcattcttct
    37021 tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt ctccagcaca
    37081 ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa gtcaacggta
    37141 tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag tcagaaaact
    37201 ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac accatggctg
    37261 ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc atgctggaac
    37321 cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc gtgctgtcgc
    37381 tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc aacctgcacg
    37441 aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc aaagttgcca
    37501 ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcgctg aacgcactgt
    37561 ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc ctgggctaca
    37621 tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg caagtgggca
    37681 gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat ctgcagaaaa
    37741 ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttccta gtttccttcg
    37801 ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat gaagagcaag
    37861 gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg gtgagtgtgc
    37921 tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc cggggatcat
    37981 tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa tcaaataccc
    38041 ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa
    38101 ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc tcacaatcat
    38161 cgcatccagc gtattcgtga caatatcttt cacaaagacg ccatcaattt tgatgatatc
    38221 agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc
    38281 aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt ctgcgttaga
    38341 aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc ggggagatgt gataacgctt
    38401 aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg gcattaaatt
    38461 gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact tcaacaagga
    38521 ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa
    38581 cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct catcataacc
    38641 ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct gatctaacgc
    38701 cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa ccatctcctc
    38761 tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca gccagcttaa
    38821 ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc aggcagcacc
    38881 atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca ggatgttaac
    38941 catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca gttcactacc
    39001 cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac ggcaaattga
    39061 gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact caagattatc
    39121 aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc ggcgcttgtt ccaacgcacg
    39181 aaagttgggt aggagcgtta acggatctgt tagcgcctgc aaatgccagc ggcgattaag
    39241 ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa aggaaatcag
    39301 caccgcgaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa agttctgatt
    39361 gtaattcaga aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag
    39421 attaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaggtaac cggcaataaa
    39481 gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg ccagccagct
    39541 taaggtaaat gcgagtgaga tagtagaaaa gcatgttgta aatcagcact gcggtgaaaa
    39601 ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag gtggatatct
    39661 ttagcgggaa atcaaagaag ctccccacaa gatacatgct gcatttgatg ccgatcggcg
    39721 tcaccaaacc aagccagaac agacgttgcc agacatatcg gctggtcaat ccataacgcc
    39781 aacgcgtccc cagctgccag cgtaatattg cgcaggcaca caacacgaca aacgtctggc
    39841 tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa gttccacaga ttggtgcaaa
    39901 acatcccgca taaaacggga acgacaccgc gccagccaaa gataaacaag attgacagca
    39961 ttacgcacag tggcatccac gccagaaaga tataactgga atggataatg gcaagtggcg
    40021 aaataaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt gtgaatatct
    40081 tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca ctgatcgggg
    40141 ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg gccagcctgt
    40201 tgccgatttt tcatccaatg atgctttcac caggcggcgc taacccggac gtagactgta
    40261 ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct ttgcagcgag ggttggaaat
    40321 ttggtggagc taagcgggat cgaaccgctg acctcttgca tgccatgcaa gcgctctccc
    40381 agctgagcta tagccccacg atgcgtttac gtaccaaatt tgctgggtgc aaaatttggt
    40441 ggagctaagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg
    40501 agctatagcc ccgtcacgta aagcttgtcg agttgacggg cggcatcata tgaattccgc
    40561 ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat cgctgaaaaa tcaggcaaat
    40621 gaacaatttt ggaaaaccgc tcgcattcag tagttattca tgtcacggtt tcctgtaaag
    40681 tggtgttata aaatgaacta ctaatagacc cacatacatt cagggaattg ttatgttcaa
    40741 ggagaggatg acaccagatg aacttgccag actgaccggc tatagccgcc agaccattaa
    40801 taaatgggta cgcaaggaag gctggacaac gtcaccaaaa cctggcgtcc agggtggcaa
    40861 agcccgactg gttcacgtca atgaacaggt tcgtgaatat attcgcaatg ctgaacgtcc
    40921 agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg ccgcttgaag tattgctggt
    40981 gacactggcg aaagagatga cgccagttga acaaaaacag tttacatcct tgcttctgcg
    41041 ggaagggatt atcggattgt tacaacgctt agggattcgc gatagcaaat aatatgaaaa
    41101 gattacgcaa taaaatgacc accgaagaac tggctgaatg cctcggtgtg gctaaacaaa
    41161 ctgttaaccg ttggaccaga gaaaaaggct ggaaaacgga aaaatttccc ggcgtgaaag
    41221 gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg cgagtttatt cagaacacgc
    41281 cagccttcca taacacacca atgttgatgg aagctgaaga gagaattgct gaatacgctc
    41341 ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc gatagataat atgacagaca
    41401 ttgagcagga aaaagtcgcg caatttttgt cacgtgaagg aattcgcaat ttcctcgccc
    41461 gtctcgatat tgacgaatca gcataaataa aaaacggcag gatattatct cctgccgttt
    41521 atctttttac acgctaatta ctgctgattt tcgcgttcag caataaaatc cagcgctttg
    41581 ttgatacgct cgatactgcg ggtcttacca attgcgtgaa cggtaacatc cagtgctgga
    41641 gactgccccg caccggttac ggcgacacgc agcggcatac caactttacc catacccact
    41701 tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt tttcagcggt ccagtcagta
    41761 atcgcggcca gtttgtcacg aaccacttcc agcggctgac gcgctaccgg acgcagatgt
    41821 tttttcgccg catcggcatc gaactcagca aaatcttcgt agaaataacg gcagctctgt
    41881 gccatctctt tcagcgtctt gcagcgttcg cccagcagtt tcaccagatc agccagctgc
    41941 gggccgttac gggtatcgat attttcctgc tcaatgtgcc actgtaagtg agtagcaaca
    42001 tactccggcg gcagcgcgtt aatgtagtga tggttcagcc acagcagctt gtcggtgttg
    42061 aacgcactgg cagatttgct gacggcattc aaagtgaagt atttgatcat ctcttcacga
    42121 gtgaagattt cctgatcgcc gtgggaccag cccagacgca ccagatagtt cagcagtgct
    42181 tctggcaaat aaccgtcatc acgatactgc attacgctga ctgccccgtg acgtttggac
    42241 agttttttac cgtcatcgcc attgatcata gaaacgtgcg cgtaaaccgg caccggcgct
    42301 ttcagggcct taagaatgtt gatctggcgc ggcgtgttgt tgatatggtc ttcgccacgg
    42361 ataacgtggg tgatttccat atcccagtca tcgacaacca cacagaagtt ataggttggg
    42421 gaaccatcgg tacggcggat aataagatcg tccagttcct ggttgctgaa ctcgatcgga
    42481 ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg ggttagcaaa acgtacaaca
    42541 cacggttcat catcagcatg atgctcatgg ctgtggcggc agcgaccgtc ataacgcggc
    42601 ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca ggcgctcttt agagcaatag
    42661 catttataag cagtgccctc ttccagcatc tgatcgatca ccgcgttgta gcgatcaaaa
    42721 cgtttggtct ggtagtacgg accttcatcc cactccaggc tcagccagtt catgccatcc
    42781 ataatggctt cgatagcttc cggcgtggaa cgctcaagat cggtgtcttc aatacgcagc
    42841 acgaactcac cgccgtggtt acgtgcaaaa agccaggagt aaagagcagt acgcgcgccg
    42901 ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag ttttgatttt catgaaatgg
    42961 ccttacgttt agaaagatgc cgacaaccgg caaatcctgg aaaaattgag tgggcgatat
    43021 tctatcactc ccgcctgatt cctcaatgta gttcgggttt ctaccttacg cattattgtt
    43081 ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca tcttgcttaa atttacgacg
    43141 aacgaacaat ttctttagaa aatgcgttga ctcattttga actctcccta taatgcgact
    43201 ccacacagcg ggggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    43261 gcggttcgat cccgtcatca cccaccaact actttatgta gtctccgccg tgtagcaaga
    43321 aattgagaag tgggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    43381 gcggttcgat cccgtcatca cccaccactt cgggtcgtta gctcagttgg tagagcagtt
    43441 gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccaatg taaaaaagcg
    43501 ccctaaaggc gcttttttgc tatctgcgat actcaaagat tcgaacctgc agcaggtttg
    43561 agttgagcgc agcgaaacaa cggagccgct cgcggcgacg gcccgaaggg cgagcgaagc
    43621 gagtcatcct gcacgaccca ccaatgcaaa aaagcgccct aaaggcgctt ttttgctatc
    43681 tgcgatttgt aagattgatt tttcttctca agttccttgt tcttctaccg atattacctc
    43741 gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc cttccataca
    43801 aacacagagt agtaacaggg catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat
    43861 aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag
    43921 tgctgaagac aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac
    43981 acaactggcg cagttgcaga aacagcaggc agaaaaggcg caggagaaag agcagcgttt
    44041 gtctctgaat gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat
    44101 ctgatacaac ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac
    44161 gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
    44221 ctggcgttca accaccggcg taatgcgctt aaccaccagt gggctacctt caggcaatgg
    44281 tagcgccagt gcaggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
    44341 cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc
    44401 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
    44461 tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
    44521 gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac
    44581 gatgccaaag tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
    44641 atgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
    44701 aagatgcgcg gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc
    44761 catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa
    44821 agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
    44881 aacaccagta tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc
    44941 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
    45001 ttgttttaaa gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat
    45061 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
    45121 ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct
    45181 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc
    45241 tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc
    45301 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg
    45361 tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
    45421 acttgtgcat tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
    45481 gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctgggg attttccttt
    45541 caccattgct ggttggtctg gtgatgaatg ttcacggtgc aaggggcagc cttgagcagg
    45601 tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt
    45661 ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca
    45721 ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag
    45781 ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga
    45841 ttgtagttaa cgtctttatg gcacgccgac tgagcttcaa taaggcagat gaaattacta
    45901 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
    45961 tccccacatc ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat
    46021 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc
    46081 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
    46141 tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg
    46201 ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtca attaagcgac
    46261 gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
    46321 ccatctcagc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt
    46381 tcctgcgcct tcgccagttt agatcctgca gcttcacccg ctatcaccag atcggttttc
    46441 ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca
    46501 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca
    46561 atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg
    46621 ctgatgacat tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca
    46681 atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc
    46741 gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt
    46801 gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
    46861 tgtgccaatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
    46921 agatctgccg gagtatggac atattctttt tcaaccagct gatcgatgat tttgtcgccc
    46981 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc
    47041 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg
    47101 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
    47161 tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact
    47221 ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta
    47281 ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
    47341 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat
    47401 ttaaacgcaa ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc
    47461 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct
    47521 tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg
    47581 ctgaccggca ccccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
    47641 tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta
    47701 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact
    47761 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
    47821 acttcaccac gcacttccag acgcgccggg atattctctc cgtgcagctt cagcggaatg
    47881 gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc
    47941 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc
    48001 agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg
    48061 aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg
    48121 cgtatctggc tgaaagctgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc
    48181 gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
    48241 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcaaagc
    48301 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
    48361 aaacccccga caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt
    48421 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
    48481 acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg
    48541 cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt
    48601 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
    48661 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt
    48721 catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag
    48781 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt
    48841 atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
    48901 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc
    48961 gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg
    49021 ctgcaccggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg
    49081 ctggaccggt tgacgcggct gcgcagacgc ataaggcggt aggtactggt gttgcggcga
    49141 cggacgagca gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg
    49201 aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt
    49261 tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt
    49321 ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag
    49381 acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca
    49441 tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca
    49501 gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca
    49561 ttgcgcaatt acccggtaaa atacgcagaa ttttcctggg attggtcaaa aggagctcat
    49621 cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca
    49681 aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt
    49741 caatattttg ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat
    49801 cccgactttc atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc
    49861 tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg
    49921 gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc
    49981 tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg
    50041 cgaatggcaa aaatttgtct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt
    50101 catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat
    50161 gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat
    50221 gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct
    50281 gtttacgatg atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcgac
    50341 ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg
    50401 ttattttgta aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt
    50461 gcatgtcatt atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc
    50521 tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta
    50581 agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca
    50641 tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt
    50701 gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg
    50761 ttgaactggt tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg
    50821 ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc
    50881 tgctgaaagc gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg
    50941 caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac
    51001 aattcagcaa tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg
    51061 aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga
    51121 ctggcgtcag ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg
    51181 ttgagccaac cgattctcca gttatcgccc aggtgctggc aggtgaagag attaaacctg
    51241 gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc
    51301 tggtcgataa agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa
    51361 tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgcc gcagcgttga
    51421 aactacaaga agatgaaagc tttaccaaca agaatattgt ggttattcta ccatcatcgg
    51481 gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac
    51541 agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc
    51601 ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc
    51661 agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat
    51721 tttatgattt ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc
    51781 agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa
    51841 aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa
    51901 cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca ttaccgctcc
    51961 gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc
    52021 tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca
    52081 gactctgggc ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca
    52141 gaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt
    52201 ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt
    52261 atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa
    52321 atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca
    52381 tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa
    52441 gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata
    52501 gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt
    52561 caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta
    52621 cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc
    52681 gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag
    52741 ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc
    52801 tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct
    52861 caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat
    52921 ccaaccaacg aagttattga taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa
    52981 gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa
    53041 gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa
    53101 ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct gcccactgaa
    53161 gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc
    53221 gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa
    53281 gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg
    53341 cgcgatcagc tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc
    53401 ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa
    53461 caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg
    53521 gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt
    53581 atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt
    53641 tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat
    53701 gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt
    53761 gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc
    53821 ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag
    53881 caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa
    53941 gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat
    54001 gggtttgttc gataaactga aatctctggt ttccgacgac aaaaaggata ccggaactat
    54061 tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt
    54121 ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt
    54181 cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga
    54241 atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg
    54301 cgaaggcttc aagcgtattg ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat
    54361 tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat
    54421 ctccaacatg gacgaaatca aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga
    54481 aaccccggtt atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgcccatcgg
    54541 cgccattttt tatgcttccg ccagcggcgg caaaatcaat tcatcgctct catgctgctg
    54601 ggtgtagcgc atcacttcca gtacgcgcaa ccccgcacgg tgcaccgcat cgtttaacgc
    54661 cttccctttc agcaaaccac tgataagctg agcacaaaac aggtcgccag tccctttcag
    54721 gtcggttttt acccgtgaat gggaaatgac attcacgctg tcggcactga ccaccaccac
    54781 ctgcatctcc tgattttctt cattaccgga ggcgctggta atcaccaccc attttaatgt
    54841 gtctgaaagc agactttttg cggcagcaat ggcactgtcg agatcgcggc aatttttacc
    54901 ggtcaggatt tccagctcaa agatattggg ggtaattccc tgcgccagcg gcagtaaata
    54961 ttgtcgatac gcctcgggaa ggtcaggttt gacataaatt ccgctatcaa tatcgccaat
    55021 caccggatcg accatgatca ataggtcagg atggtctttg cgtagcgcag tcagccactc
    55081 ggcaaggatt ttgatttgcg atgccgttcc catatagccc gtggttacag cacgaagttg
    55141 gcgcagcgca tcacgctcct gaagcgcacg caaatagccg ctaaaccatt cgtccggaat
    55201 cgcaccaccg tagaaagtgt cataatgcgg cgtattgctc agcaataccg tcggcacggc
    55261 aaagacattc aagccgttct gttttatagc aggcaccgca atgctgttgc ctacgcttcc
    55321 gtacactacc tgcgactgca cggcgacgat atccgcctgc agcgccctac tcttatcgtt
    55381 aaacaacaac aaactactca tttaattttt tctccttgcc gatgatcctc atcgtattcc
    55441 aaccgaaact ttacctgatt ctggcagtca aatcggctat tacaaaacaa agataaggta
    55501 attcagtgaa gaaaatcatt tgtctggcca ttacgctgct aatgacactc cccgcttacg
    55561 cgaagttaac tgttcatgaa gaagcccgca tcaacgccat gctggaggga ttagcacaga
    55621 aaaaggattt gatatttgtg cgcaacggtg atgaacatac ctgcgatgaa gcggtttccc
    55681 acctgcgtct gaagctcggc aatacccgta accgcattga cactgccgag cagtttattg
    55741 ataaggtagc ttcgtcgtca tcgattaccg ggaagccgta tatcgtgaag atccccggta
    55801 agagcgatga gaacgcacag ccttttttac atgcgttaat tgcgcagacg gataaaacgg
    55861 tgcctgcgga aggaaattaa tccgctttgg taaggcattt acaggaggta acatgaaaaa
    55921 atgctttatt tatcacgatg aaaaatcgaa taaattttgg tggatagatt acgaagggga
    55981 tagtttagct gtcaactatg gcaaggtagg tagtattggt aaattccata caaaagaatt
    56041 cgataatgaa gaacagtgtc tgaaagaagc cagtaaattg attgccgcaa aaatgaaaaa
    56101 aggatatcaa gaaactccag actttaactt catggatctc tactattttg atgatgaaga
    56161 gattgggtta catcttaaaa cgtcacaccc aaacttccag tgccatttta ctgatccact
    56221 ttatatgtgt tgctgggatg aagaatctcc ttttggtagc gatgaaggtg ctgatgctct
    56281 aaacgaactt gaaaatagcc tccgttctgg actgtgctga tttccctcaa atgttaattg
    56341 aaactatgtg gggtatgaaa tacatcgcta tggacagtat tcttgaagag gatgtgcgtg
    56401 cgcaattact agccgatgaa atgagctcta tccagagcaa tatgattacc tacgcgactg
    56461 cattcggtca gatcaaagtc atgggtaaaa tctcccataa acttaaaaag atgggactca
    56521 atgcactagc gcgtcatcag cttaccgcaa aaattcttca atggggtgac ggtcaggact
    56581 caccaatact tcaaaaaatg attgatgacc ttactgcttt tccacacgaa aattaaaaac
    56641 tgcatttgtc ggcagcaaca actgccgaca aatcagatta ttgacaaagt gcactttgtt
    56701 tatgccggat gcggcgtaaa cgcctgatcc ggcataaaaa tattgctaat tcaatatatt
    56761 gcagaaacat tgtaggcctg ataagcggag cgcatcaggc agttttgcgt ttgtcatcag
    56821 tctccaatgc tattaatcct taaatccctg ccccctgact aaaatgctct tccccaaaca
    56881 ctccggtgga gaggtagcga tcgccacgat cgcagatgat cgctaccacc accgcaccag
    56941 ggttagcttt tgccacccgc agtgctccag caaccgcgcc gcctgagctg acgccacaga
    57001 atattccttc ccgcaccgcc agttcgcgca tggtgttttc cgcatcgcgc tgatgaatat
    57061 ccagcacctc atccaccagc gcagcgttga aaatccccgg cagatattcc gcaggccagc
    57121 ggcgaatgcc cggaatactg ctgccttctt ccggttgcag gccaacaatg gtcaccggtt
    57181 tggattgttc gcgcataaag catgaaacgc cggtgatagt gccggtcgtc cccatgctgg
    57241 agacaaaatg agtgatgcgc ccgccggttt gctgccagat ttccggcccg gtggtggtgt
    57301 aatgcgcata agggttatcg ggattattga actgatcgag cagctttcct tcgccacgat
    57361 tcgccatctc cagcgccaga tcgcgcgcac cttccatgcc ctgctctttg gtgacaagaa
    57421 tcagttccgc accataagca cgcatcgccg cacggcgttc ctggctcatg ttgtcgggca
    57481 tcagcaattt catgcgatag cctttcagcg cggcaatcat tgccagcgca atgccggtgt
    57541 taccactggt ggcttcgatt aagacatcac ccggtttaat ttccccgcgc ttttccgcct
    57601 cgacgatcat cgaaagtgcc gcacgatctt tcaccgaacc tgccgggtta ttgccttcca
    57661 gttttaacca cacttcactg ccgttatccg gccccattcg ctgcaacttc accagaggcg
    57721 tattgcctat tgtttgttct aatgtactca caatctctat ccatacgtgg tgttgcctga
    57781 tgcgacgctt gcgcgtctta tcaggcctac aggttacaaa ccttgccatt aaaaagcccg
    57841 gatcgcggga agcgcctccg ggcgtttaac attcactcaa cctatcaggc gctttgtgcg
    57901 agagcaagtt cctcatcgcg ggtttcaata cgctcgtcgc cgttatacag ccgcgcatgt
    57961 tgcagaccaa cgaataaacg ctcgccatgc tgcggggcat cgtcgccatg catcacgacc
    58021 gtcagcggtt cgttgtacca ccccagcggc tgcaccacta attgggtgta gtgacctttc
    58081 gggctggctt ccagtacctg taccggcagc ggcgaatcga gactggtacg gcggctgata
    58141 tccacttccc acgggcgcag gaagagatcc accggcccct gatacgcagg tgtgtagccc
    58201 agcggccagc gatgcgcgcc aacatggaac tgcccgccgc gaatggttcc ctgcaggcgg
    58261 ttcacttcgc ccataaattc gagtacaaaa cgggtcgctg gttcgcgcca tacctgattc
    58321 ggcgcatcag cctgttcaat attcccctga ctcatcacca ctacacgatc ggctacttcg
    58381 gtcgcctctt cctggtcgtg ggtcacaaac acgctggtga atttcagctc ttcatgcagt
    58441 tgacgcagcc agcgacgcag ctctttacgc acctgcgcat ccagcgcgcc gaacggttca
    58501 tcaagcagca gaatttgcgg ttctacagcc agcgcgcgcg ccagcgccac acgctgtttc
    58561 tggccgccgg aaagctgcgc cggataacga tccgccagat gcgcaagctg gaccatttcc
    58621 agcaattttg tcactttcgc tttgatggct gcggcattcg ggcgctcgcg acgcggcagc
    58681 accgtcaggc caaaagtgat attgtcgaat accgtcatat ggcggaacag cgcgtaatgc
    58741 tggaacacga aaccgacttt acgatcgcgc gcgtgcaggc gactcacgtc ggtgccgtgg
    58801 aagcgaatat gcccgctggt ttgatgctcc agcccggcga taatccgcag cagcgtggtt
    58861 ttcccggaac cggacggccc cagcaacgcg accatctgac cagaaggaat atccagtgag
    58921 atatcgttca gcacctgggt gcgaccaaaa gacttcttga tattggcaat ctcaatgctc
    58981 atgatgctcc tcctgctgcg cgcgtttttc ctgattctcc aggcgccact gcaacatact
    59041 ctttaaaaac agggtgataa tcgccatcaa cgtcaacagc gcggcggcgg taaaggagcc
    59101 gacggtgttg tagtcctgct ccaacaattc aatctgtaac ggcagcgaaa gtgtttcgcc
    59161 gcgaatcgag ccggaaacca ccgacactgc accaaactcg ccaatcgcgc gggcgttggt
    59221 cagcaccacg ccgtaaagca gcgcccagcg aatattcggc aatgtgacgc gacggaacat
    59281 ctgccagccg gacgcgccaa gcaaaatcgc cgcttcgtct tcctggctgc cctggcttaa
    59341 catcaccggc accagctcgc gcaccacaaa cggacacgtc acaaagatgg tgaccagcac
    59401 cattccgggc caggagaaca taatttgcag attatgttca tccagccagc cgccgagcgg
    59461 gccgttggag ccgtagaaca gcagatacac cagaccggca accaccggcg atacagcaaa
    59521 cggaatgtcc agtagcgtca gcagtaactg gcgtcccggg aagttaaagc gcgtcaccag
    59581 ccaggccagc agaatgccga acaccaggtt taccggtacg gcaatcagcg cgatcatcac
    59641 cgtcagccag atggcgtgca gcatgtccgg atcggccaga ttctgtaaaa ccggcatcag
    59701 ccccttgctg aatgcctgca cgaagatgta aatcatcggc accagcagga tgaacgccga
    59761 aaccagcatc ccgatgccaa tcagaaacca tttgccccag ttaatcgggc gcgcgtcata
    59821 acgcttcaat tgggtaactt ccgccattaa tgacctacca cacgccgacc aaagcgactt
    59881 tgcagagtgt taattgagaa cagcagcagc agagatgccg cgaggatcac cgaagcaatc
    59941 gcgctcgctg ccgggtaatc aaactcctgt aagcgcacaa aaatcatcag cgacgtcact
    60001 tccgtcttcc acgcgatatt tccggcgata aaaatcaccg cgccaaattc accaagacta
    60061 cgggtaaacg acagcgccac gcccgccacc agcgccggag aaagctccgg cagcaccact
    60121 ttgcagaaac tctgccagcg cgttgcacca agcgtttccg ccgcttcttc atattccggg
    60181 cctaactctt ccagcaccgg ctgcacggta cgcaccacaa acggaatgct ggtaaaggcc
    60241 atcgccaccg caataccaag ccaggtatag gtgactttga tatcaaactt cgccagccat
    60301 tcaccgtaaa aaccgtttac ggaaaagagc gaggccagcg ttaaaccggc gactgccgtt
    60361 ggcagcgcaa agggtaaatc catcagcgca tcaagcagcg tgcggcccgg aaagcgataa
    60421 cgtgttaaaa tccacgccat cagcagaccg aaaacgccgt taaaaatcga tgccacaaac
    60481 gccgacagca gcgtcacttt gtaggccgcg accacctgcg ggttggtgat cacctcccag
    60541 tactgcgccc agctcatctc agaaagctgc atcaccagcg cagagagcgg cagcagcaat
    60601 atcaggcaca caaacagcag actggtgcca aggcttaaag taaagcccgg cagcacgcgt
    60661 cttgaagaaa ctgcaaacat tacttacgcc ccgccgctaa cagcttgtct aactcgccgc
    60721 cgctggtgaa gtgggttttc atcacttccg gccatgagcc aaatttgtct tccacgcgga
    60781 acagttcggt ctgcgggaat ttatctttca gtttgtccat gacttccggg ttattcacac
    60841 ggtagtaata gtcggtgatg atggtctgcg cctgtgggct gtagagccag ttcaggtagg
    60901 cttttgcggc tttttccgta ccgttggcct gcacattttt atcgacccac gccaccggga
    60961 attccgccag aatattggtt ttcgggatca ccacttcaaa gccctgtgct tcatactgtt
    61021 tacggatgtt gttcacttcc gattcaaagc taatcagcac atcgcccaag ccacgctcgg
    61081 caaaagtggt ggttgcgcca cgaccgccgg tatcgaacac ttcaacgttt ttcaggaact
    61141 gggtcataaa ctgttcggtt ttggctttat cgccaccgtc agctttatcc gctgcgcccc
    61201 atgccgccag ataggtataa cgcgcgttac ccgacgtttt cgggttcggg aaaatcagct
    61261 tcacgtcgga gcgcaccagg tcgttccagt cgtggatatt cttcgggttg cccttacgca
    61321 ccaggaagcc catggtggag tagaacggcg agctattatt cggcaggcgc gactgccagt
    61381 cagccgggat cagcttgcct ttgtcgtgca ggatttgtac gtcggtcacc tggttataag
    61441 tgacaacgtc tgcttttaag ccctgcaaaa tcgccagcgc ctgttttgat gacccggcat
    61501 gagattgttt tatcgtcagt ttgtcgccgc cgttatcttt tgcccattgc tgttcaaacg
    61561 gcggattcag ggcggcaaac agctcgcggg agacgtcata agaactgttc agcagttccg
    61621 ttgcctgtac atggcccgcc agcagcagag aagcgaccag cgcgagtgag ttctttttca
    61681 gtaagttaac ggccattgcg cacccttata aatttaatga ctttctaata gccatcatat
    61741 ttataacgga cgtgaaagga gtaacggttt tatataccgt ttggtgattt ggaagttaaa
    61801 aagggaataa gaacttggtt caacaggcgt aattgttgca ggcagtttgg acacggacag
    61861 cgcggagaaa ccggagcgta cacatagtac gtgaggattt cgagcactgc ccggggccaa
    61921 aatgacaaat gaaatagcct ggtgaactta gttcaagacg aaatcctccc cacacatgca
    61981 gggaggaaac agaggtgaat cagataccga cgctaaccgt ctccggcagt gtgctgccgc
    62041 catcaatcac attctgtgta ccggttaaat agctggattc atccgatgcg aggaaggccg
    62101 ccagttcgcc gacttccagc ggatcggcga ggcgacacag cgggattgct tttgccattt
    62161 cagtcagcac cgattctgga tcttccgggt tcgactggcg ggcaatgctt tccgccattg
    62221 gcgtgcggac gtatcccggg cagatggcgt taacgcgaat accggattgc gcgtactcca
    62281 ccgccagcga tttagtcagg ccaacaatcg ccgctttcgt taaggcatac gccgtttcgc
    62341 caggatcggc caccatatca ccagtgactg aagacatcat cacaatgcga ccatctttgc
    62401 gcgcaatcat ctccggcagc accgccttcg tgacgttcca tacgccttta atattgatat
    62461 caatatggaa atcgcgatct tcatcgctca tatcgaggaa actgcccaga cgacaaacgc
    62521 ctgcgttatt caccaggata tcaatgcgcc cttctttttc cttcgcgcgt ttgatagctg
    62581 cggctaccga cgccgggtca cgcacatcgg cgacaaccgc cgtacagcga tgaccacgac
    62641 cacacagttc gtccgctagc ttttcgatct caggggagat atccagcaag attaggttcg
    62701 cgccatgacg tgcaaaagtt ctggcaattc cttcgccaat tccctgcaat gcgcccgtaa
    62761 tcagtgctgt cttgcccgtg agtttaccca ttttaatgct ctccttgtta atacagagcg
    62821 ttacactcca ttccctctga aaatacagga taaacaaaat attgctgcgg atcagctcac
    62881 taattcagga aaatctcaat ccagtcgctg ctttatgcat catttcaggc gttgtgttag
    62941 ctcactgctg cgctgaatca ttttcaatga cgccacatca ttgatgttgc acaagaccaa
    63001 caaacagtaa atcggtaaca gaattttgcg ccgtgcgggt agacatcgac gagctacgcc
    63061 attcggtttc accggaaacg gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt
    63121 cagccagaga agtaatggca atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca
    63181 agacaatctc ttttttactg ccgctgtaag agatagcgat ttgtacatcg ccttttttca
    63241 acgcctggga aacggtggct tgtacgtgcg tgtcggcttc gcaggccacg cgatagccaa
    63301 ttttcatcag tttgaacgac agatcgcgcc ccaccagcgc cgatccgccc aggcccgtta
    63361 tctggataaa cggtgcttta ctgatgacct caatgatttt ttgcagccgc gcgtagtcga
    63421 acaacgcaca ggtttgctcc agcgccagct ctttttcgcg attcagtttg cgggcaatca
    63481 cttccagcga atcatcactg gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt
    63541 tctcccggct ggcactgtat tcgccaatta acgccatacg caattcagta aatccttgcg
    63601 cgccgagctt ctgggcaaat ttcacaatgc tcgactggct gataccgagc tgtttcgcca
    63661 tctgccgtga agagacggat ttcagctcgc tcactctggc ccgcagaaag tcagcaattt
    63721 tttgctcgtt cccggtaaat tcactttccg cattgcgtat tttcgtcagg tacaacatct
    63781 tccactctcc tgggctggca gcaaaaattc cgcttaagtt cttatgacgc tcttcacact
    63841 ctgcgagtgt agactcaatg attcctttaa gtctgtcaat tcgggatata tgattccata
    63901 catctgcaat tatttgatcg taaatagtaa ggtcaccacc gatgcaacta gaaaagatga
    63961 ttactgaagg ctcgaacacc gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt
    64021 gccggattat caacgatgaa gataaaaccg taccgcctgc cgttgatcgc gtcctgccgg
    64081 atatcgccgc ggcgatcgat gttatccacg cccaggtcag cggcggcggt cgtctgattt
    64141 atctcggtgc gggaacatcc ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca
    64201 cctacggcgt gaaaccgggt ctggtggttg gtttgattgc tggaggcgaa tatgccattc
    64261 agcacgcggt ggaaggagct gaagatagcc gggaaggtgg tgttaacgat ctgaaaaata
    64321 ttaatttaac ggcacaggat gtggtggttg gcattgctgc cagcggtcgc acgccgtatg
    64381 tgattgccgg actggaatac gcacgccagc tcggctgccg cacagtggga atttcctgta
    64441 atccggggag cgccgtttca accaccgctg agtttgccat tacaccgatt gtaggtgccg
    64501 aagttgttac cggttcttcg cggatgaaag caggtacagc gcagaaactg gtgctcaata
    64561 tgctttccac cgggctgatg attaaatccg gcaaagtgtt cggcaacctg atggtcgatg
    64621 tggtcgccac caacgaaaaa ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg
    64681 gatgtaatgc cgaacaagcg gaagcggcgt taattgcctg cgagcgcaac tgtaaaacgg
    64741 ccattgtgat ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac
    64801 acggcggctt tattcgtcag gttttagaca aggaataacc catggccaaa gagatcagca
    64861 gtgaacttct gaacaccatt cttacccgtg tcggcggacc gggaaatatc gccagttgtg
    64921 gtaactgtat gacgcgcctg cgtctgggtg tacatgacag ttcactggtt gatcccaata
    64981 tcaaaacgct ggaaggcgtg aagggcgtca ttttgaccag cgaccaggtg caggtcgttt
    65041 ttgggcctgg taaagcgcat cgtgccgcaa aagcgatgag cgagctgctg ggcgatgcac
    65101 cggtacagga tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa
    65161 cctccggcgt gcaacaattt ctcgccaaat tcgccaccat cttcacgccg ctgatccccg
    65221 gttttattgc cgccggtctg ctgctgggga tagcgacgtt aattgccacg gtgatgcacg
    65281 ttccggcaga tgctcagggc acgctacccg acgcgctgaa ttttatgaag gtgttcagca
    65341 aaggtttgtt caccttccta gtgattctgg tgggctataa cgccgcccag gcatttggcg
    65401 gcacgggcgt caatggcgca attattgccg cactcttttt gctcggttat aaccccgctg
    65461 caaccaccgg ttactacgcc ggttttcacg atttctttgg tctgcccatc gatccgcgcg
    65521 gcaatattat cggcgtgctg attgccgcgt gggcctgcgc ccgcattgaa ggcatggtgc
    65581 gccgctttat gccggacgat ctcgacatgc tgctgacctc gttaatcacc ctgctgatca
    65641 ccgctacgct tgcgtacctg atcattatgc cgctgggtgg ctggttattc gaaggtatgt
    65701 catggctgtt tatgcacctg aacagtaatc cgttcggttg tgcggtttta gccgggctgt
    65761 tcctgatcgc cgtggtattt ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa
    65821 tggacagcca gggattcaac agcttatttc ccatcctttc aatggcaggc gcgggccagg
    65881 tgggcgcggc gctggcactc tactggcggg cgcaaccgca cagtgcgcta cgcagtcagg
    65941 tacgcggggc gattattccc ggcctgctgg gcgttggtga accgctgatt tacggtgtca
    66001 ccctgccccg catgaagccg tttgttaccg cctgtttagg cggcgcggcg ggcggtttgt
    66061 ttatcggatt gatagcctgg tggggtctgc cgatgggctt aaacagcgcc tttgggccgt
    66121 ctggtctggt ggcgctgccg ctgatgactt ccgcacaagg catccttccg gcaatggcgg
    66181 tttatgctgg cgggattctg gtggcatggg tttgcgggtt tattttcacc acgctctttg
    66241 gctgccgtaa cgtcaatctg gactgatatt atgaaacgga caatgctcta tctttctctg
    66301 ctggctgtta gttgtagcgt cagcgccgca aaataccctg ttctgacaga aggtagttga
    66361 tcaagtatat gcggcgttaa agcagtaata aaaaggccag tcagtaatgt tactgcctgg
    66421 cctttaatca atgaatcaga aacgcttaca gcgccatcaa cttgtccagc gacggtgcga
    66481 aataatagcc gccggttacc ggtttggtga aacgcaacat cgcatcacgt ttgccatcgg
    66541 tatcgccaaa catgctcagc agttgctgct caatgttatg cagacgcgcg cagtaggcgc
    66601 agaagtacag accgtgagtg ccactggcgg tgccgtacgg caggctctgg cgaacaatct
    66661 tcagcccttt gccatcttct ttcagatcaa cgcgggtgag gtgagaggtt tccggacgtt
    66721 cgtcgccgtc aatctcttcg ttggcctctt tggtgcgccc gatcatcatc tcctgatcgt
    66781 gaacgctcat ccggttgagc tgcttcaggt tgtgctccca acgctgcaca aacacatagc
    66841 tgccgccgga atccacgccg tctttgataa ccgccacttc gcggcgcgtc tcttcacccg
    66901 ccgggttttc tgtaccgtca acaaagccgc tcaggtcacg ctcttccacc caacggaagc
    66961 cgtggatctc ttctttcact tcaatgcagt caccaaaggc ttccatcgcc gcctgagcga
    67021 cagagaagtt tacgtcgtga cgcagagaaa gaatgtggat caacacatcg aactgggtcg
    67081 tcggcgcaag gcctttaccg tagcccggaa aatctttcag ctcttctgcc ccaacgccgc
    67141 cgctcagagc gcgccaggtg ttgttaccaa aggcaaccac cgcaccaaga tgcgcgtccg
    67201 ggaatttcgc ttcaaaagtt gccagtttgt cggcaaatgt tttactggcc gcacgcaggg
    67261 cgtcaacttc ccctttcacg ttggcttcga tccaaatcgc cgcgcggcaa tgttctggca
    67321 aaatgccact ctgaacctga gacattgttc ctcctgaaaa taagaatgcc acgcaatccg
    67381 tggcattgtt gagcgctatt ttacgtgttt tttcgcgacg gcggtttgtt cagacgcaaa
    67441 ttaacgccgc cagataattt tactgacttt ccagtttttc agggtatcgt ccgaaggcat
    67501 taacccttca ggaccacgcc attccccgct gaactggtag ctaatatgtt gactgccttc
    67561 ggctttacat tcgacggcac gattatcatc accatcagct ttttggcaat tgccaaatgc
    67621 tttgctgtaa aggtcgctaa acggtgtacc gattttaaca ccggtgtcag caggaatatc
    67681 gctatccagc acgtcaatgc gactgatcgt gccttgatcg ccgttaatca ccatcgccac
    67741 gttgtcgcct ttcatcactt caaaaaagcg caccacgttg ccgttcgtgg ttttcattcc
    67801 gctgcgcagg cgataatcgc catcaagcgc atcagcaatg gcttgttctt gcagtggtgt
    67861 ggacgccgtt aattcaccca cgccctgctc gctcactttg gtggacgatc caaaccagtt
    67921 ccacgggtta gcggcagacc agttaactga cgacatcgtg gaacagccgg tcagcatcaa
    67981 cggcatagcg cataacatta aacgcagcga tttcatgtca cttcctttgg ttattcaata
    68041 acgttgcttg gagtgcaaat tcaccaaaaa gtgccgttat tctttttctt cattaaaaca
    68101 agcgcgtaaa cgtcgattgg tcagcagcca gattaacgcc acaatatccg ccactaccag
    68161 cgccagaccg ataccgctaa cggattcacc gttcagccac agccacggtt gccagcaaag
    68221 taaaaccacc tgcgccaaca gcaacagaaa atagagcaca tgccaggtgc gaggaaacga
    68281 agcccgccga ccgctcaaca aaaacgccag caccgccgga ataccgggaa tcagccccag
    68341 ccagaaatta tcgtgatcgg gataaaacag atttagcagc gcagtaccct gctcacgcga
    68401 cgcaccggca atgacaaaca gcacccaggt tcgcgcctga agcaatagca caagccagaa
    68461 gagcaagggt aaacgcaggc gtccgtgcgc atcataatgg acaggatgga actcagtact
    68521 cttcatcttc aatcaaacgc ttacccagac tcagcacgtc ggcgtgttca tatcccaggc
    68581 gttcatacat tccgagcacc atgtcgttat cttccggcac gttgatctga attttcgggc
    68641 agccgcgagc aatcagcttt ttctccagcc ggttaagcaa cgcattggca attccacgcc
    68701 cacgaaactc tggatgcacg ccaagataat aagcagaccc gcgatgcccg tcataaccgc
    68761 ccatcaccgt tccgaccact tcaccgttta cctcagcgac caaaaacaaa ctgacgtcat
    68821 ggttcatctt acgctcgatg tccatttccg gatcgttcca cggacgcagc aagtcgcaac
    68881 gctcccaaag ggtgatgacc tcttcgaaat cttcctggcg aaatacgcgt atctccatgg
    68941 tattcgttac ctttttgcgg gttaaaaggc tgattatggc gtgaacggtc gaattagcca
    69001 atatctgacg aaaatcggtt gaaaaagtgg cataatgggg agttgtcaac tattgaaatg
    69061 aaaagtaaaa caattctcaa cagcaaaccg tcgtaacgga ttacgcgata cgatataaca
    69121 tctggaactt tattattaca actcaggccg tatgagcact tttaaaccac taaaaacact
    69181 cacttcgcgc cgccaggtgc tgaaagccgg tttagctgcc ctgacgttgt caggaatgtc
    69241 gcaagccatt gccaaagacg aacctttaaa aaccagcaac ggacacagca agccgaaagc
    69301 caaaaaatct ggcggcaaac gtgtcgttgt tctcgatcca ggccacggcg gaattgatac
    69361 cggagcgatc ggacgcaacg gttcgaaaga aaaacatgtg gtgctggcga ttgctaaaaa
    69421 cgtccgttcc attttgcgta atcatgggat tgatgcgcgt ttaacgcgtt ctggcgatac
    69481 gtttatccca ctttacgatc gcgttgaaat cgcccataaa catggcgcag atctgtttat
    69541 gtcaattcat gccgatggct ttaccaaccc gaaagctgcc ggtgcttcgg tatttgccct
    69601 ctctaaccgt ggggcaagta gcgcaatggc gaaatacctg tctgaacgcg aaaaccgcgc
    69661 cgatgaagtt gccggtaaaa aggcgactga caaggatcac ctattgcagc aagtactgtt
    69721 tgatctggtg caaaccgata ctattaaaaa cagcctgacg cttggctcgc atattctgaa
    69781 aaagattaag ccggtgcata aactgcacag ccgcaacacc gaacaagccg cgtttgtggt
    69841 gttgaaatcg ccgtcggttc cttcggtgct ggtggaaacc tcgtttatca ccaacccgga
    69901 agaagagcgg ctgttaggca cggcggcgtt tcgtcagaaa atcgccacag cgattgctga
    69961 aggcgtgatc agttatttcc actggttcga caaccagaaa gcacattcga aaaagcgata
    70021 agttatgaaa cccgacgcac accaggttaa acagtttctg ctcaaccttc aggatacgat
    70081 ttgtcagcag ctgaccgccg tcgatggcgc agaatttgtc gaagatagtt ggcagcgcga
    70141 agctggcggc ggcgggcgca gtcgggtgtt gcgtaatggt ggtgttttcg aacaggcggg
    70201 cgtcaacttt tctcacgtcc acggtgaggc gatgcctgct tccgccaccg ctcatcgccc
    70261 ggaacttgcc gggcgcagtt tcgaggcgat gggcgtttca ctggtagtgc atccgcataa
    70321 cccgtatgtt cccaccagcc acgcgaatgt gcgttttttt attgccgaaa aaccgggtgc
    70381 agagcccgtt tggtggtttg gcggcggctt cgatttaacc cctttctatg gttttgaaga
    70441 agacgccatt cactggcacc gcaccgcccg tgacctgtgc cagccatttg gtgaagacgt
    70501 ttatccccgt tacaaaaagt ggtgcgacga ttacttctac ctcaaacatc gcaacgaaca
    70561 gcgcggcatt ggcgggctgt tctttgatga tctgaacacg ccagatttcg accactgttt
    70621 tgcctttatg caggcggtag gcaaaggcta caccgacgct tatttaccaa ttgtagagcg
    70681 acgtaaagcg atggcctacg gcgagcgcga gcgcaatttt cagctctacc gtcgcggtcg
    70741 ttatgtcgag ttcaatctgg tctgggatcg cggcacgctg tttggcctgc aaactggcgg
    70801 gcgcaccgag tcgatcctga tgtcaatgcc gccactggta cgctgggagt atgattatca
    70861 gccaaaagat ggcagcccag aagcggcgtt aagtgagttt attaaggtca gggattgggt
    70921 gtaactccct cacccccatt cccgcatccg ctgatgcaac gtcaacgacg gcttctcggc
    70981 aaacagctgc tggtaatccg tggcaaattg ccccagatgc cagaatcccc actgcatggc
    71041 ggcgtctttt accgttgtgc tttgcgacca cgggcttatc agttcgcggc gtacgacgtt
    71101 caggcgaatg cgtttcagcc acgcgtttgg gccaatgcct aaaatagcgt gaaacgcgtt
    71161 ttgtagcgtg cggcgactga catgcagttg attacacaaa tccagcaccg tcaccggttc
    71221 ggacatgttt tccagcacat attcacgggc gcgggaaagc aaccgacggt aactctgatg
    71281 actgatgctt tccgccgtca ccattggttg cgcttcttcc agcatcgccc ccatcgccat
    71341 tagcaaatta tcccccagca cttttcgcac tgcaggctga tggagatttt ccgggttctc
    71401 gcaaaacgtc gccagcgcct gttggacaaa gccccacagc gcggctttat gctgctcttt
    71461 cacttccagc gccgactggc tacgcagcat atgtaatacc cgatccggat tgtgcaaaaa
    71521 gttagcctga caggtgatga catcttcaga cagcactacg cccaggatcg tataatcgtc
    71581 cggcgtactt aattcaaact cggttccacc ggggcgcgtc gctatttctg cgcttcccag
    71641 acattgcgaa ccgataaatc cctgctcacc gcgcgtcgcc ggaatgccaa accagaacga
    71701 gttcggccag accaggcacg actgacgcag cgccagaccg gtgtattcac gaaaaacctg
    71761 aatatcatcg agtagaattt ccgtaaattc accatgaaac ttgcccggat gcagctgatc
    71821 gtaaatctgc tgctaggcgg taatcgtcaa agcatgttca tagacatccg ttgtctgtcg
    71881 ttgatgaaca ttatccactt cgaccttcgg cgtgagcttc aggttttcgg gtaagggttc
    71941 atgataaaga tggtgcaaat tggctgtacg ggtctttttc atgatgttaa tgccgggtgt
    72001 tgtaggacac ccggcacctc cgacaggtta atggggcttg agacgataac gactactgcg
    72061 tttacgtaac gtcccggcag aaaagagctg ttcgagcgca tttctggctt tttccagcgg
    72121 ccagccaaag tgggcggcca cttctcctgc cgtcattccc tgacatactg atgttaacag
    72181 cgccaacaac tcgtcagcag attccgcgac gataactggc gcgtcgggtt cctttacggg
    72241 ttgcttcggc tggcggttaa agccagtaac cagccactgg gtgtcatcgt ctggccgacc
    72301 gatctcctta cggctgatga cacgcccggt acgcatcgcg gcagcacaac ctgcgtccag
    72361 cgccgcacga cacgccgcca gatcgccttc caccaccagc gttaagcgac cagggtcaag
    72421 cacttcgtga ctgagcagac gaacgttagc tgctttgagc atggcatccg ctgcatctat
    72481 cgcggcgacc atgccgtcca cttccagcaa tcccagtgca ttgatcatcg gcaacctccg
    72541 ttacgcacgc tggattggat tacgcgcgat ttccagcact gcatcggtaa aggcgttaca
    72601 ggctgcttta cacgcggcct ggctaccggt taaaaatgct gccgagtagt tggtttcaga
    72661 cggtggcggg acgtaggtca ccagctgtac gtcggcagat ttcaacgcgg catcaatgcc
    72721 gtaggtcgct tccagcggcg gtgccaccag atacgccatc ggatcgccaa gcgtgatccc
    72781 ggcggttgat gaaagataag aaccggtacg cgaaaccaca tgcgccagga aggcggtgtt
    72841 ttctgcgtca ttggcccact ggaaagctgc gccattttca atatgtgcaa ccatcgcatc
    72901 cagaccggca cgcacttccg ccgggtttgg cccaccaagc ataatcagca cttcaccggc
    72961 agtcggtgac gggccgtgtg ccgctcccgc atacagcgag cgaccatata ccacttcgac
    73021 catcgcttgt ttggtcgctt cgtcagccgc aatgtaggtt acgtcgtcag aatcggcaga
    73081 aatcagcccg aggctacgaa tatgcggcgg caattttagt tcacgcgcaa attcagcgtt
    73141 aacggaggca atcacccgca tggcggttac cgacggtcga atcaaatcta aagctggcat
    73201 gatgcctcct tatcgggtca tgttgatgcc ggatgctttc tgctccagca tacgtttcgc
    73261 caaatccaca atgacggcac agcagcacga aagagaagca aaagtcagct acctggtaca
    73321 ctaggcagcg ggtcagcaaa ggggggcaaa tgcccctttt ttcatgtcag ataatcagat
    73381 cattacctca ataaactaat aatcacatcg cggttacaac cagtttgata gtgatagctc
    73441 aaacccagac gtggcgctgg ctgcaagcaa aataagccgc aaaaatgcga ccccatgttt
    73501 actagccgca aaaatgcggt cctatttgcc agaacctttt attattcaat tggttagtgc
    73561 aagaaaaagc gtgattactg ttcaagaagt atccagaaag gtcatgattt tgacgctgaa
    73621 aaaaaatagg tgggcatttt tgcccactca ctagccgcaa atttgcccac tcactagccg
    73681 catttttgcg gcccaaagta ataaatacag agaaataata aatacagatc agcgccaaga
    73741 tttttcgtct tttcggcgga atcatgagac tagtgcttac gtcctgataa cctccagagc
    73801 agaccttaac ttctgtgcag caggactacg ctcattgccg ctcggctagg tcaaatcctc
    73861 acgataaagc tgttcggatt gtgaccataa tgttaaagga ctctttaaat cattccttga
    73921 cgaattaact agctggctta agcggaatag catgcaggat aaaaacaaaa ccagcttagc
    73981 agtggggaga gcttcgcttc gctcgtctcc tggggccaat gctcaatgaa acgacgtaag
    74041 gcacggagcg ctcaccatag gtagacacat aaacatctga gagcgcacct tctatcgcgc
    74101 tacgcgctca ataaaaacca acgacaccgc cacactgcaa gaccaatggt ttttatcgcg
    74161 tatacagacc agacaggaaa cagcagcaaa gcagcgtata gccaggtgca gacagcacac
    74221 tcacacgacg cacagcaggc ttaagtgatc actcaggaaa ctttatcccc gccgccgatc
    74281 accgcgaact atcatgaccg cgccaaattc gaaaggaatg gcccatagaa cgcgatgaac
    74341 acacataagc gctacactta cttaacgtcg ccacagagcg attgagagcg cttaaaatcg
    74401 atgcgatttc attaaatttt tctcactcgc ctgaatggtg gcgatcagga ctgatcgcaa
    74461 cattgccccg cctcgatatt gagcgcataa tagatacata cattaagcgg aaaagagata
    74521 agtttattgt gaaaatcagg gggttacata tcatgcgcat atcagaacaa caacggcaga
    74581 tcctcgacta tctggcaaaa aacgaatcaa tcaccgcacg acaagccgca gatctcgttt
    74641 atggtggcaa tgttacccgc gtccaggttg acgccgcccg ccgctcactc atgacgatgg
    74701 ttagtaatgg gctaatcagg aaacaaggcc gcgctttcgt agctgaaaga catatcaatc
    74761 acgcttatag ccaggacgtt gaagaattgc gccgccagat cctttgggta ttcagcaaac
    74821 agggtcagga gtatttcctt tctgaacacc caaatttcgc cgccaaagag ttaacagtaa
    74881 gcggattgtc gtgcgcactg gattatcttg tcggtgaagg ttgcgttact tacaagcagc
    74941 tatggcacgc cctgaatacg cttgtagaag atggtttact cattgccacg cgtgagaaca
    75001 tcgaatactg gataatgccg cgttcaggtc gccgcaatca tgcacggtat atttcaggca
    75061 acagatacag aacgccggac tttaatccga cacaggaaga tcgggaccgt gccgaaatga
    75121 tgtgcaggga acaggagaaa agaattatgg ataagttatt ttcgatgcag tgaacacaag
    75181 cgggcgcaat gcccgttttt attgccttat tccacgcctg acgaatcaat ttgatcacac
    75241 ctgagaaaat gcatagccag aagttaaggc cgttatattg cgattgggag cgttttaaac
    75301 ggtatcaatt taattaattc tgatgtggtg atttgcaggc aaaaaaaacc cgccggagaa
    75361 gcgggaaata gagacatatc gttgtagtta ttaacagatc actttgaggt cgagggcggc
    75421 taaccctcgc taatattatc ccgctattac cttgcgggtg caaataatct tttattgctt
    75481 tgatgattta ataaatagca ctttttgcta acaaaaaccg ctgtcaatat atggccaaaa
    75541 atgtcaacag aaaaaataag gtttataata ttaagtatca catgggtaat gggttcaggg
    75601 aatcggaatc caccgcctca cggaaattca aaatttgctt cttctttcct ccgtgggcgg
    75661 cttctttttc gtatcgaggt atttatgaaa gcaataacat tagaacagtt cgcagcaatg
    75721 gcgaatgtat cgaccagaac gctgaggcgc tggctaaaaa aaccatcaac cggagtccct
    75781 ttgccattta ccccacgcgg tactcgccaa ttattctggg agcacgaatg caaagaatgg
    75841 ttgtacagaa cagaacgata ataatcacgc atcacatttt gaggtaaaca tcatgaccaa
    75901 tatcgaaaga ttagaacgct gtgccgatat tatgcgacgc agatggattt atgaccctga
    75961 agcgggttta ttgtttagcc gtgaaacaaa agacgttatc aaaggcagca tgaccaataa
    76021 agggcatttg gttgtaacgg tacatgaagg taaattcaaa gtgacactca cctatcagaa
    76081 agcctgctat gtatacacat acggcgctta tgacgagaca ttatatgaag tgcaccacgt
    76141 taatttgaac aaacaggata accgcatcaa gaatattcgc ctgatgccga aagaaaagca
    76201 ccgccggata cataaaaacg tccgcaaact tattcggctg ggccacacac aatggcttca
    76261 accgcaatta attgaacaat cactataaac acaggagtaa ataccatgca ttcattagac
    76321 agttattttc agcgcacgac agcccctaaa tcagccgccc aggaacgccg tgaggaattc
    76381 catgagaaag tgatgcgttc tgctgattat attgcggaca agttcgtgga aacggttcgt
    76441 ccacttgttg atgaagtagc tgataaactt cagtctgaaa tgccggaaga tatggaaggc
    76501 actgcaaagc ggcgactcat ttgcgaatta agtcgtcgtt ttggcgtgtc aataagcgct
    76561 tttaaataat cgaaaaaagg ggggaaagat aatggacgca tttgaggtac tggcaaataa
    76621 aggcgctgaa ctggtagcac agcgtgataa aaccgccaac gaaggggaac gcgcactgct
    76681 aaataaacag attaaagcga tccgtatggc ccagtttaaa ttaatcagta atgaagttat
    76741 cgaaacaatt aaaccgcaga tcccacaaat cgttgctgac gctattaaag ccgccggact
    76801 ggagaaacgc attgccgggc taaaaacgaa cgcagagaaa atggagatgt acaaacagag
    76861 cggtgctccg aatcccgatg agtacttctc accggatgtt gagtttatgg cgcaggttga
    76921 ggaacgtctg ggtagctgtc tcaccgagga acagcgtcgc tattttgatg gagtggatag
    76981 ttcggcaggt attgacctga actcgtattt cggtcgtgaa attgagcatt ttgatgccgc
    77041 cccgtctatg ccggagccag aaccagaaat ggccaccgat cctgaagctg agcaacggaa
    77101 acgcgtattc aatgcgatag ggattcggta ctgatcggat aaaagaaagc cgggaattac
    77161 cccggctgac ttaacaatgt gaccaatgaa caaatcggac aagacaaaca ggataagcag
    77221 tctcgaaaca atgttcggga actatattag cacaagtccg gagaaaatca tcatgttggc
    77281 actacgcatc atgcagggca tcgctaagac cctcgcagaa catgtcttag acctgaaaca
    77341 ttcgccactg agcaaacagg ccatgaagcg ccaaacgctt cgtctgtggg ctgaatattc
    77401 gcttggaact atcaataaaa tcatcgacat gaaatcgggg ccgagcaacc agagcgcgga
    77461 ggaaatggag ttcatcaggc gtttaattct cattcgcaga gacattcata gccagttaca
    77521 cagcgtggga atcgatatta atgatgggac gggcgattaa ttttcggaca agtactatgc
    77581 gcgcgcgagg agctgaacat gaagcaagat attgcagaca ggctggagat actcgaaggc
    77641 cagcgggcag aagcgaaaca actacgtaag caggccagaa gagcgcacag gaacaacgaa
    77701 gcagagttac taaccaaata catcagtttc accaattact gcatctacga gtgttacaag
    77761 gaagatgcag aggactggct ggattcattg ccagaacagt actgagcaga ataacaccac
    77821 gtcatgttgt tctgaggtga gcatatcacc tctgttcagg tggccaaatt tagtgtgcca
    77881 cttcagcatc accgttgaaa tcacaataac gatttttttg taaatatgct ctcacatatg
    77941 taggaataga ccaggttggc attgaaacac atctaccatg accataataa agtaattttt
    78001 tgatatgttt atggttttat attaattaaa atatatctaa atttgatagt tttaaaattg
    78061 caaaatccag attgtgtttg taatgtttaa tctgattgta ttgagtggcg tttattatga
    78121 aaaataaatc aggtaattct tgttatagag aagcagtcga tattcatagc tcacttgagc
    78181 tttcatttat attgcataaa tatcatttgc gtgtagatga gaagaaatct cgaaatgtat
    78241 tcagcgccat acgtgatgag ctcatccttg atggggattc acgacaaaac ttagctactt
    78301 tctgtcaaac atggcttgat gatgaaatcc gtgagttgct taatttagct attgataaaa
    78361 acatgattta taaagatgaa tatccacaaa tggcggaacc tgaaggtcgc tgtgtgcgta
    78421 tgctttccga ttcgtggagt ttccccgatt cgcgacacac cttaggttgc tcaaccattg
    78481 gttcatctga agctgctatg ctcggccgct tggcgttaaa atggcagtgg tgtaaaaaac
    78541 gcgaagtaca aggtaaatca accgaacctg atttgcgatc ctgtgcaaat atgctggcat
    78601 aaattctgcc gttactgtga tgtgaaattg tgtgaaattc cacttgatga tcatcacctg
    78661 ataatgaatg ctaaagaggt tttgaagcat gtcgatgaaa acactattgg tgtcgttacg
    78721 actttaggta ttacttattt ttgtaaagca gattgcgtta gtgtttttaa gataagtgtt
    78781 aaagttaata ttagttttta tatttacatc gtaattatat gtttctattt taaaaatgtt
    78841 cgagagtata aaaatgtcta atgctaatat cgcacccaaa agtaagaaaa tgacagtggg
    78901 gacactcgct atcatgaata taactgctgt agtaagtcta cgatttatac cttctgaagc
    78961 agaatacggc ttaggtgcca ttttttacta tacctttgct gcaattatgt ttttagtacc
    79021 tgttgccttt gttgcggctg agctagctac aacatatcca caaaaagggg gggcatttcg
    79081 atggatagga gaagcatttg gggctcgctg gggttttgta ggaatgctta tgacctggct
    79141 acaagtcatc ccctactttc caacagtact gacatttggt gcagtctcgg ttgcatttat
    79201 tgatgttgat atgggggtgg cagaatcaat tgccagtaat aaatggtata ttttattttt
    79261 cgtactattt gtttactggg gagctatttg tgttgcatta cgtggcgttg gtattttctc
    79321 gcaggtatct aagtggtgcg gtattatagg gacaattatt ccagctatta tccttgttat
    79381 tcttggtttc tcctatctgt ggttcagcgg taaaccagcg gtgatcccat taggttgggg
    79441 tgatcttttc cctaatatag ctaacttcca gaatattgtt cttgccgcca gcatatttct
    79501 tgcttatgca gggatggaaa tgaatgcggt ccatgttaac gatttagata atccaactaa
    79561 aaaataccca attgcaatta ccatcgcttc tataggtact attgcaatat tcctgctcag
    79621 tactttaggg gtcgccttta ttattcctac agataaaatt agtctaactc aaagtttgtt
    79681 ggttgcctac gatatgctgt tcgaatgggc tggagtgcca tggcttggct ctgtgatggc
    79741 atttatgcta gcaattggcg tactgggtgg ggtggttact tggattgcag ggcctaatac
    79801 aggtgtatta gcgatcgcga aagctggtta tttacctaag tttttccaaa aaaccaatcg
    79861 tcatggaatg gggcaccatc taatgtttgt acaaggtata attgtctcag ttctttctgt
    79921 tacttttgtg ataatgcctt cagttcaagc agcttttcag attttatcac agttaacagt
    79981 tatgctctat ttagttatgt atatgctcat gttcgctagt gctatctatc tacgacattc
    80041 tcaacctgat acaaaacgcc catatcgagc ccctgcttta tttttctggg caacacttgg
    80101 attcttaggg tcttttctgg cttttgtatt aagttttatt ccgcccgagc aaatcccaac
    80161 tggtagtcca accagctatg ttctttattt gttagtatta gttgccctat ttttattaat
    80221 tccaatatta atttacgcct tccgaaaatc tgactggaaa gatccaaact ctgatttcga
    80281 accattttct tgggaaaaaa gaactagtaa ttaaattttc cataagctac aaaagatgag
    80341 gttaaatatg tttagtgctg aatctccaat tggtgtaata gactcaggtg tcggtggatt
    80401 gacatcggta aaggaaataa ttaatttgct tcctggtgaa gatattatct attgtggaga
    80461 taatatgaat gcaccttatg gcaaccgttc tgccgatgat atcatcagtt tgactaaaaa
    80521 aatgctgact tttttacagt caaggaatgt caaactagtt gctgtcgcat gtaatactat
    80581 atcctcaact ttggaatccg aagaatacag cggatatgct aagagctttc cgtttcctat
    80641 cttatccatc attgaacctg cagtagaaga tgtgattcgc cagcaatata aaaatgttgg
    80701 tattatcgct accgagttta ctattaaaac ggggtgtcac aaagagctaa taaaaaaatt
    80761 gaattcaaca ataaatgtat ttggagaacc aagcaaaaat cttgcaatgt taattgagga
    80821 aggtaatctt aatgctcctg caatcttgaa tgacatcaaa aaacatgtta accatttaat
    80881 ttccctccat ccagtaaatg aaattattct cggctgtact cactatccaa ttgttcagaa
    80941 tttttttgaa gcagttgcac cggatataaa atttattaat cctgcacatg atcaggctat
    81001 ctctattaaa aatcacttgg gacagctaaa tttattaaac tcctcaaata taggaacttt
    81061 gcaaataaat actagtggaa gtatggagat ctacaaaacg gtactagggg aattgagcat
    81121 tacgaaacct catacattca gtatcaggca gttttaaatt tatccggtac accgtaaaat
    81181 cccgtccttc tgaactgcac cccaaaagtt ggacaccaac tgagtaaggt gcattttatg
    81241 gacaaaccca gttacatact gggtagtgca tccaattagt agaacatgtg tttttcgata
    81301 aacgctccga tcactttttc gtggatctca actgagcgtg agaagcagat tgttttacga
    81361 cccaaccgct taatgcgggt gcgtagcgtc agattattac gctcaatgcg ttgggtgaat
    81421 attttgccgg tcagatgctt attcttcggc acctcccggc catagctgcc ccagtcatcg
    81481 ctggtcagca tgccgatgtt gaagggtgta agcagtgcca gtagctcccg gcacgtttga
    81541 tcggttcggg gaccaaaagt gtaggccagt acaccgcctg ttttggtgtt gtacgcgtac
    81601 ccgagccaat gttgccgggc tttactgcca acgtagctcc attgctcatc aagctcgcag
    81661 ataaacgcca catcagcatg ggcaacaggc gaagaagtta ttcgctttgg cgcgagtttt
    81721 ttaaagtccg gatgacggtg ttactaccaa ttttcagtgt cctggcggta tcgcgaaccc
    81781 cggcaccatt aaaggccatt tcagttatca gctctttcat acccggctta cgtgcttgat
    81841 aagtataagt gaactgaaac acacggtggc agtcacggca gcgaagtctg tcacggcctt
    81901 tagggttctg accatggcgg taaacctgag ctgactgaca acggggacaa tgaatattaa
    81961 cgctggtcat gagataacct caaaagcccg tattatacat cagattcaac taattagagg
    82021 catcacccat ccagcattat aaaacatact aacaataact aaattataat aatcacaact
    82081 tacaatcaaa taataaatgc ataagattaa caatgatttt ttcgggttat atttatctaa
    82141 aaaagcagaa cattatgtaa cttattattt tctaagcgaa aatttattgt gcggtcagac
    82201 taacaaactt gcgtaccacc cgaagaacat gcgaagattt aagtattctt tcttttttgg
    82261 tttttatttt tcattttctc ttttcttctc tttccggttc tgttctggtt ttggtttttt
    82321 cctttttatt tttattcttt cttcagggtt atgctaaaac accaaaacat ttttcaccac
    82381 aaaagcatcc aatcatcttg ccagaccaat caccttaacc ggatacactg ccggatataa
    82441 tcccactaac acccagacac caagcaccat acctgataac taccgtattc aggaaggaaa
    82501 tgcgagtgat ttcaccgtca ccggcacaac cgatccgcca aaaagaggcg taccaatgtc
    82561 gatatagtcc cccgcgcgga caatcacttc gtcaatgact gccaacggga gttgttgtag
    82621 ctgtgggcgc aacaacatgc ccagagcttt accaaagtcc tgcccggcca ccaccagcag
    82681 gggatgcgga ttcggaaaac gcgcgacgaa atcgaccagc gcgttgatga ccgtcagaac
    82741 cgcggcgtaa cgcacaggca gcgaggcggg aagcgccagc acgtacacgt cagttttggg
    82801 atcaagatcc agctgaagca gcgcctgttg ccaggcactc accagatccg tttcatcaat
    82861 cgggatcgcc accggcaaat tgcgcagcgg cagttgtacg ccctccagcc agattgtgct
    82921 gccagagagc gaaagggtat gtgcacccgc gccaatcacc gtggagcgta cggtttgcgc
    82981 cggaaactgc acattcatct cacgcaggcg cggatggtca tgcagcgccg ttgccagaag
    83041 tgggccaata tcggcaaaac agaacgggtt ggcgggctgg tggcgataac attcgcccac
    83101 gcctccagaa agcgtaatga tttcgggcgt aacacctgcg ggcagcaaac cggtttgcat
    83161 caatgcctgc gcgagcggcg aaagcgttcc gtcaatcact tcgacaatca gcgccgccat
    83221 ccgccgggta acctgcacca gctgcgcgcc ggtcagcgaa cgggcgtcag tgcctgcacc
    83281 gaagcactca tccacaatca tctgccccgg tttatgagcg taaaccacgc gcccctggct
    83341 gtcggtttcc agcaggcgac caccgacgtt gagacaggca gtgccgctga tttttccggc
    83401 atcgaacagg gcgtagttcg cggtgccacc gccgatatcg atattcagta cccgacacag
    83461 ccgttgttca gaaagggttt gcgccccggc tccgtgaccg gcgatcacgg attcgaggtg
    83521 cggcccggcg ctggcgacga caaaatcgcc cagcgactgt ttttccttca tcactttttc
    83581 catcagctgt gcgaccaggc tttcggtaaa ctggccttcc ggcagctggg caatgatggt
    83641 ttcgcggatg cgctggcttt cggttttgtc ggctggcacg ctggcgggca cggacgccgt
    83701 taccgggatc gattcatcac attcggtgat ggtaaaaccc agtagatccg ccacttcacg
    83761 ggcttccggg gtaataatgc tggcgcgcag aaccacggac attgcctgtt cgccgcgcgc
    83821 gtgtgcttca cgaatatcat tcgctgtgat gagttttttc acctgcttcc tcctgtttgg
    83881 tgagagatgc cagataatcc accagttgct gcacgctttg cgggtcgtgg ctgtttagct
    83941 cgaaaatcgg ctcttcgaaa cccgtctcaa gcaataactt tcgcgttgcg gcgacgtcag
    84001 catctggcat gtccgtttta ctgatgacgg cgatttgccg tttgctgacg ccaatatcca
    84061 gcaacccggc aggcaaccga ctttccgggt catttgcgcc atggacatag atcagcatgt
    84121 ctacatcctg tagcgtggta attaaggcgt gataccagcg gggatggcta aaatattcac
    84181 ccggcgtatc aatatcgccc ttatcattaa attccacggc cagtgttttt ctggcgaggg
    84241 tataattccc ctgcaacgca ttaaataacg ttgtttttcc tgccccgacg ctacccacaa
    84301 acgcaattcg tttcatgaat gcctcgtatt aacttttggt catttcacaa agcgtataat
    84361 ttaataagcg acctaaaccg ctgacggttt gtgataacgc ttcttctact gcgcccaccg
    84421 agccataaat caccaatgca ccgctgaagc ggtcgagaaa accgatatgc acatcagcgg
    84481 ctttcagcgc taaatcaccg gcgatcatcg ccgtttcgcc gggagttagc gtcataatgc
    84541 caattgcgcc cgcatccgga acgccgattt tcttcgccag ttcctcaccg ggatgcgcga
    84601 tgagatgcgc cagtgtgacc tgtttgccgg gcacaaattc ctgaatgatg cgttctttat
    84661 ccatgagtcg ccgcctgtga aaaactttcg tgtatcgtga caatttttcg tcagagtaaa
    84721 taacaaaatt cggcaagcgc gtttaaaagg tgaggtagat caataagaat ggagagatat
    84781 ttactgttat gaataaactt tcatatgcga cgcatgaata taaataaaat aatatcgaga
    84841 tattttatat actgattttt ttattattcg caaactcagt aataagcttt gctaatatat
    84901 ttcaggaaaa ccgcttaccc tgaatattca gggtaagcgt gagagttaag aatgaattac
    84961 agcggttggg tttgcgcttc aaccacggcc agcgccacca tattgacgat acgacgcacc
    85021 gatgcgatcg gcgttaacac gtgaaccggt ttcgccacgc ccatcagcac cggaccgaca
    85081 gtcacacctt ccgagctgga aacgcgcagt aagttgtaac taatgcgggc agcttccatg
    85141 ttcggcatca ccagaatatt ggcagaacct ttcaaagggc tgtccggcat acggtcgttg
    85201 cgaatcgctt ccaccagcgc tgcatcgccg tgcatttcac catcaatcat cagatctggc
    85261 gcacgatccc tgaccagttc cagcgcctga cgcattttgc tcgacgacgg gcagtcagaa
    85321 gaaccaaagt tggagtgcga caacagagca acgcgcggct caataccaaa acgacggaca
    85381 gtttctgccg ccatcaaggt gatctccgcc agctcttctg cgtctggttc gtcattgacg
    85441 taggtatcgg caataaaggt gttaccactc ggcagcagca gcgcattcat tgcacctgcg
    85501 gtgtgaacgc catcgcgata accaaagaca tttttcacca cactaaaatg ttcatgataa
    85561 tcgcccaccg taccgcagat cattgcatcg gcttccccac gctgaaccat gatcgcgccg
    85621 atcactgtcg ggttactgat cagcgcacgc tgcgcctgtt cctgagtgac accgcgacgc
    85681 ttcatgatct ggaagtattc ggtccagtac tctttaaagc gcggatcgga ttcgttattg
    85741 acgatttcaa aatcaacgcc cgctttgatc tgcaagccca gtttctgaat gcgcatttcg
    85801 atcacgttcg gacgaccgat aaggatcggt ttcgccagtc ccagcgttac cagttcctga
    85861 gtcgcatgca gaacgcgcgc ctcttcccct tccggcagaa ctacgcgctt cggcgctttg
    85921 cgagcctggg agaaaatcgg cttcataaac aggttggttt tgtaaacgaa ctcagtcagc
    85981 ttgtcgatgt agacgtcgaa atcagcaatc ggacgcgtcg ccacgcccga ctccatcgcc
    86041 gcttttgcga ccgcaggagc gatcttaacg atcaagcgcg gatcaaacgg ttttggaatg
    86101 atgtattccg gaccaaagct cagatcctga acgccatacg ctgaagccac cacttcactc
    86161 tgttctgcat gggccagttc tgcaatcgcg cgtaccgccg ccagtttcat ctcttcgttg
    86221 atggcggttg cgccaacgtc cagcgcgcca cggaagatga acgggaagca aagaacgttg
    86281 ttcacctggt tcgggtagtc ggaacggccg gtacagatga tggcatccgg acgcacttct
    86341 ttcgccagcg gcggcagaat ttccggttcc gggttcgcca gcgccaggat cattggtgcc
    86401 cgagccattt tcttcaccat ttcctgggtc agcactttcg ggccggaaca gcccaggaaa
    86461 atatccgcac cttcaatcac atcatcgagg gtacgtttgc cgtcatccac caccgcatat
    86521 gcggctttgg tttccgccat atttggctca cggccctgat agataacgcc ttttgaatcg
    86581 caaaccacga tgttatgttt ttgcagcccc agcgctacca gcaggttcat acaggcgatt
    86641 gctgcggcac ccgcgccgga aaccaccatc cgcacgtcgg agatgttttt ctccaccacg
    86701 cgcaagccgt tgaggatggc ggcagtgctg atgattgccg tgccgtgctg atcgtcgtgg
    86761 aataccggaa tattcatccg ctcgcgcagt ttctgttcaa tatagaaaca ttctggcgct
    86821 ttaatgtctt cgaggttgat gccgccgaag gtcggttcga gcgcggcgac gacttcaata
    86881 aatttgtccg ggtcgagttc atcaacttca atgtcaaata catcaatccc agcgaatttc
    86941 ttaaatagaa cgcccttgcc ttccatcacc ggtttgcctg ccagcgcgcc aatgttgcct
    87001 aaccccagca ccgccgtacc gttagagatc accgcaacca ggttaccgcg cgcggtatat
    87061 ttgtaggctt ttagcgggtc tttttcgatt tcaagacaag gtgcggcaac gcctggtgag
    87121 taggccagcg ccagatcgcg ctgcgttgcc agaggcttgg ttggagaaac ctggattttc
    87181 cctggaactg gaaattcatg gaaatcaagt gcactttgtt ttaactggtc atccatttgg
    87241 ttgttccttt cacgtaacgt tcacaaataa agtgtgtggg caacagcccc tgcccacaac
    87301 gtggcgcaca ttattaccct gccggagtct acagactttg agcaagtcca aactctcacc
    87361 attaatataa tgttttggta ataatcctat aacactgatg ttacctgctt aatccagcaa
    87421 taccatgcct gtctgctatg cttttttgat gcgtttagcg aaatttctca gaagtgtgaa
    87481 ttaacgcact catctaacac tttacttttc aaggagtatt tcctatgaac gagttagacg
    87541 gcatcaaaca gttcaccact gtcgtggcag acagctgcga tattgagtcc attcgccatt
    87601 atcatcccca ggatgccacc actaatcctt cgctgttact caaggctgcc ggattatcac
    87661 aatatgagca tttaatagac gatgctatcg cctggggtaa aaaaaatggc aagacccagg
    87721 aacaacaggt ggtcgcagcg tgtgacaaac tggcggtcaa tttcggtgct gaaattctca
    87781 aaatcgtacc cggttgcgtg tcaacagaag ttgatgcacg cctctctttt gataaagaaa
    87841 agagtattga gaaggcgcgc catctggtgg acttgtatca gcaacaaggc gttgagaaat
    87901 cacgcatttt gatcaaactg gcttcgacct gggaaggaat tcgcgcggcc gaagagctgg
    87961 aaaaagaagg tattaactgc aacctgacgc tgctgttttc ttttacccag gcgcgggcct
    88021 gtgcggaagc aggcgttttt ctgatttcgc cgtttgtagg gcgtatttat gactggtatc
    88081 aggcacgcaa gccgatggac ccgtatgtgg tggaagaaga tccgggcgtt aaatcggtgc
    88141 gcaatatcta cgactactat aagcaacacc actatgaaac cattgtgatg ggcgcgagct
    88201 tccgtcgcac cgaacaaatc ctcgccttaa ccggctgcga tcgactgact atcgcaccga
    88261 atttgctgaa ggagctgcag gaaaaagttt ctccagtggt acgtaaatta atcccgcctt
    88321 ctcagacgtt cccacgccca gcacccatga gcgaagcgga gttccgttgg gagcacaatc
    88381 aggatgcgat ggcggtagaa aaactgtctg aaggcattcg tctgttcgct gttgatcaac
    88441 gcaaactgga agatcttctt gccgccaaac tataaaccag ccacggagtg ttatatgtcc
    88501 cgaaaagacc ttgccaatgc gattcgcgca ctcagtatgg atgctgtaca aaaagccaat
    88561 tctggtcatc ccggcgcgcc gatgggcatg gctgatattg ccgaagtgct gtggaacgat
    88621 tttcttaaac ataatcctac cgacccaacc tggtatgatc gcgaccgctt tattctttcc
    88681 aacggtcacg cgtcgatgct gctctacagt ttgctacatc tgaccggtta cgacctgccg
    88741 ctggaagaac tgaagaactt ccgtcagttg cattcgaaaa ccccaggcca cccggagatt
    88801 ggctatacgc caggcgttga aaccaccacc ggcccgcttg gacaaggttt ggcgaacgcc
    88861 gtcgggctgg cgatagcaga gcgtacactg gcggcgcagt ttaaccagcc agaccatgag
    88921 atcgtcgatc acttcaccta tgtgtttatg ggcgacggct gcctgatgga aggtatttcc
    88981 cacgaagtct gttcgctggc aggcacgctg ggactaggca agctgattgg tttttacgat
    89041 cacaacggta tttccatcga cggtgaaaca gaaggctggt ttaccgacga tacggcaaaa
    89101 cgttttgaag cctatcactg gcatgtgatc catgaaatcg acggtcacga tccgcaggcg
    89161 gtgaaggaag cgatccttga agcgcaaagc gtgaaagata agccgtcgct gattatctgc
    89221 cgtacggtga ttggctttgg ttcgccgaat aaagcaggta aggaagaggc gcacggcgca
    89281 ccactggggg aagaagaagt ggcgctggca cggcaaaaac tgggctggca ccatccgcca
    89341 tttgagatcc ctaaagagat ttatcacgcc tgggatgccc gtgaaaaagg cgaaaaagcg
    89401 cagcagagct ggaatgagaa gtttgccgcc tataaaaagg ctcatccgca actggcagaa
    89461 gagtttcccc gacggatgag cggtggttta ccgaaggact gggagaaaac gactcagaaa
    89521 tatatcaatg agttacaggc aaatccggcg aaaatcgcta cccgtaaggc ttcgcaaaat
    89581 acgcttaacg cttacgggcc gatgctgcct gagttgctcg gcggttcggc ggatctggct
    89641 cccagcaacc tgaccatctg gaaaggttct gtttcgctga aggaagatcc ggcgggcaac
    89701 tacattcact acggggtgcg tgaatttggc atgaccgcta tcgccaacgg catcgcgcac
    89761 cacggcggct ttgtgccgta taccgcgacg ttcctgatgt ttgttgaata cgcccgtaac
    89821 gccgcgcgga tggcggcact gatgaaagcg cggcagatta tggtttatac ccacgactca
    89881 attggcctgg gcgaagatgg tccgacgcac caggctgttg agcaactggc cagcctgcgc
    89941 ttaacgccaa atttcagcac ctggcgaccg tgcgatcaga tggaagcggc ggtgggctgg
    90001 aagctggcgg ttgagcgcca caacggaccg acggcactga tcctctcaag gcagaatctg
    90061 gcccaggtgg aacgtacgcc ggatcaggtt aaagagattg ctcgtggcgg ttatgtgctg
    90121 aaagacagcg gcggtaagcc agatattatt ctgattgcca ccggttcaga gatggaaatt
    90181 accctgcaag cggcagagaa attagcagga gaaggtcgca atgtacgcgt agtttccctg
    90241 ccctcgaccg atattttcga cgcccaggat gaggaatatc gggagtcggt gttgccttct
    90301 aacgttgcgg ctcgcgtggc ggtggaagca ggtattgccg attactggta caagtatgtt
    90361 ggtctgaaag gggcaattgt cgggatgacg ggttacgggg aatctgctcc ggcggataag
    90421 ctgttcccgt tctttggctt taccgccgag aatattgtgg caaaagcgca taaggtgctg
    90481 ggagtgaaag gtgcctgatg gtgatcgccg gatgctgtat gttcccctca ccctaaccct
    90541 ctccccagaa gggcgagggg acggtccgtg cgctttttag ggccgcatct gcgattttgt
    90601 ttaacgggtg atccacaacg tgggcctggc gtctggccca tgccagttat cgcaggtagg
    90661 ttctgcggcg taacgcacca ggcgaaaacg ctgaccgtca aagcgccagc gcgcctgaat
    90721 gccacaatcg cttaatccgc gccctttcgc taaggtcacc agttcacgcg atttctcatc
    90781 aaatgttgcg ttcatcagtt ccagttcatt cgtctcctgg ccgttgttga acggcaaacg
    90841 caaccgaacc gggcgcgagg ctagcggctt tttacgcgac acaatccatg ccaaatcaat
    90901 ggtgttatag gcccctgcct cacagctaat catcatcagt gctttatcat cggtcagcgc
    90961 agtgacattc acctcacgac gtaacggatc aagcgagcag cgcagaccat tcatccgcca
    91021 gtttccataa tccagcaaat cgttacgttc ttcgagtgag agtggcgtcg gcgttgggtt
    91081 aaccaccgcg acctctttca gcgcaggcgc gggcggtacg ctgagaggcg gttcgtcccc
    91141 tttcttgatc cacgcggttt cactgccaac gcgcttttgc tgagcatcaa taaacaacaa
    91201 cgccgctttt aagccgctca gagaaatggt ctgattgccg tcgcgtaagg tgatcgcctt
    91261 cccttcctga attatctgca aaaacgcggt gatggttgcc gtatcatcgg ttactaataa
    91321 ccatggtgaa atccgccact tgtcaccact taatgccaac ggctcaccat ctaatagcag
    91381 ccgtggcgct atctcccctt cagacgcatc cggcgacttc aacccaccgc gctcaatacg
    91441 taaaacggca tcggtatgcg ccccagcgct gcggctcagg gtcatcacca gtccattatg
    91501 atcgcccgta ttgcgcgcca cacaaaaatt ttgattatta caggtgacct gccagtcgga
    91561 aaacgcccgt tgcgctggtg ccgcccacac caaagacgtc ggcaacaaag cgaaaaaaaa
    91621 gagaaggaaa atgcgatagc gcatggacgg tacgacccca gaagttcaaa caaacagcac
    91681 aagtcgtatc ttcgttgcgc aggtagcgcg gctcaatcgg atttgtcgga ttgagccaat
    91741 aataaacaac gaaatattat tgctattttt cagtccatta aatgtgacat ttgcaaatag
    91801 ttaagcaata acaccgtctt accgtcacgt atctcgccgg ttttgatcat ctccagcgcc
    91861 tggctgaacg gcagctcgag cacttcaata tcttcatctt caacaccgcc accggcgtta
    91921 gcgcgctgat tgtcactgta ttcggcgata aaaaagtgga ttagctcagt cacaccgcca
    91981 ggcgacatat acagttcaaa taatttgcgc acttcgccca cttcatagcc cgtctcttca
    92041 atcgcttctt tacgaatgca cacttccggt tcgtcgttat ccagcagccc ggcgcaggtt
    92101 tcaatcagct gcccgctttc attgccatta acccaggtag cgacacggaa ctgacgaatc
    92161 agaaccacgc tctttttctt cgcgttgtac aggaggatcg tcgcaccatt accgcgatcg
    92221 taaacttcac gtttatggcg gataacttcg ccatctttgc gcgtgagatc gtaagtaatg
    92281 ttgtgcaggg tgaaatagtt atcggagaga attttgtctt taatgagggt gatttgttgc
    92341 gtcataccga ctccacagcg cgaaatgaac aattatatta cgctgtgaag tcgggtttgt
    92401 ctgcgcaggc tatcagtgag atttgactga ttttaccccc agccagtcaa taatcccctg
    92461 tgccgcatga cgtccttctg ccattgcggt aaccaccaga tccgcaccac gcacggcgtc
    92521 gccaccggcg aagattttcg gattggtggt ctggtaacgg tactggcttt ccacatccgc
    92581 gatgatgcgg ccccatttgt ctaccgttac accgtgcgac tccagccacg gcatcccgtg
    92641 tggattgaag ccaaacgcca taatcaccgc atcggctggc atgacaaatt cactgccctc
    92701 aatcggtaca ggtcgccgac gcccctgggc atccggctcg ccaagacgcg tgcgcaggaa
    92761 acgaattccg caaacgtgac cttgctcatt cagctcaagc gccaccggct ggacgttaaa
    92821 ttcgaagttg gccccctctt cgcgggcgtt cttcacctct ttcttcgagc ctggcatgtt
    92881 agcttcatca cgacgataag cgcaggtgac gttactcgcg ccgtggcgca gcgcagtacg
    92941 cacacagtcc atcgcggtgt cgccgccgcc cagtaccacg acgttaagtc cggcggtatt
    93001 gataaacggc tcttccggta gctcttcgag ccccatcacc tgtttagtgt tggcaatgag
    93061 gaacggcagc gcgtcataaa cgcccggcgc atcttcattg ggtaaacccg ctttcatgga
    93121 acggtaagtg cctacgccaa cgaagaccgc gtcgtattgt tccaaaagca aatccaaaga
    93181 gacatcttta cccacttcac aattgagttc gaagtgaatc cccatcgcgc tgaagatttc
    93241 ccgacggcgt gccagcaggg atttatccag tttgaaagaa ggaatgccga aagtgagcaa
    93301 gccaccgatt tctggatggc gatcgtacac cgtcaccgcc acgccattgc gggtcagaac
    93361 atccgcacag gccagccctg ccggacctgc cccgataatc gccacccgct tgtccacttt
    93421 ggtgacatga cttaagtcag gacgccaacc tttcgccaac gcctgatctg aaatatagcg
    93481 ttcaatgttg ccgatagtta ccgcgccgtg ctcatcgcga atagtacagg caccttcaca
    93541 caaacggtct tgcggacaaa cgcgtccggt aatttccggc agggtgttgg tctggtgaga
    93601 aagctcgacg gcggcgtcga tgtttccggc tttcaccagt tcaatccact gcggaatatg
    93661 gttatgcagc gggcaggtcc attcacaaac gctgtgctcg ccgaacttaa ggcagcgcga
    93721 ggcttcccgt tgtgcctggt cggcgcgaaa tggcagataa atttcatcaa aaccggtttt
    93781 gcgcgcttca atcgccagtt tatccggctc gccacgcgcg ggcgttgcct gcatttgttc
    93841 gactttactc attaccggca tttcttgtgc agcggtactg gcatgccacg gttgatgctc
    93901 ctgacgtgcg gtgcgcaagc ggcgggattt cgccatgccg gagagtgcga cgtcagtgac
    93961 cagttgcagc gcgtccgccg ggcaattctc gacacaggca ggaccgtttt cgcgccctgc
    94021 acaaaggtcg catttatgcg ccgtggcttt tacttttcct gccgcgacgg gtgtcaggac
    94081 gatttgcatc gtaccaaacg gacaggccac cacgcaggat ttacagccaa tacacttttg
    94141 ctgattgacc tgaatgctgt catcaacgtg gctgattgcg ccattagggc agctacgggc
    94201 gcagggcgca tcttcacaat ggtgacaggt cactgcacta cgttgctgct gatgtttgat
    94261 aaccgtaatt cggggatgaa aatggtgttg gctcaggaca tgttgctcat cattgtgagc
    94321 catgacacag gcgatttcac aagcatgaca acccagacat tgctgactgt tggccataat
    94381 aaaacgattc ataacgacct tcttttttgg ttgtaaaaac cttattcttt atatgagtgt
    94441 tgttattacc cgacttacag gggaaccggc aatgttcatg tgcccagaat aagtaactat
    94501 ttcgctataa actgtggcag atcaaataat cccatcactg actaaattgc gtttcagcga
    94561 actggaacat taatgatttt ttgtggagaa gacgcgtgtg attgttaaac gacccgtctc
    94621 ggccagtctg gcccgggcct ttttttacat tgtgctgctg tcgattcttt ccacgggtat
    94681 cgctctgcta actctggcga gcagtttgcg cgacgctgag gctatcaata ttgccggatc
    94741 gctgcgtatg cagagttacc gcctgggcta cgacttgcaa agtggcagtc cacaactcaa
    94801 tgcacatcgc cagttgtttc agcaggcact gcattcaccg gtattaacca acctcaacgt
    94861 ctggtatgtg ccagaagcag taaaaactcg ctatgcgcat ctgaatgcca actggctgga
    94921 gatgaataat cggctcagca agggcgattt gccgtggtat caggccaata ttaataatta
    94981 tgttaatcag atagacctgt tcgtgctggc tttacagcac tacgctgaac gcaaaatgct
    95041 gctggtggtg gcgatttccc tggctggcgg tatcggtatt ttcacgctgg tcttttttac
    95101 tttgcgccgc atacgccatc aggtggttgc cccgctgaat cagctggtta ccgccagtca
    95161 gcgtattgaa cacgggcagt tcgactcgcc gccgctggat accagtctgc cgaatgagct
    95221 tggtctgctt gcaaaaacct ttaaccaaat gtcgagcgag ctgcataaat tgtacctttc
    95281 gctggaggcg tcagtagaag aaaagacccg cgatctccac gagaccaagc gtcgtctgga
    95341 ggtgttgtat cagtgcagtg ttcccaggcg ctgaacacca gccagattga tgtgcattgt
    95401 ttccgccata ttttgcagat tgttcgcgac aatgaagcag ctgaatatct ggagctgaac
    95461 gttggggata actggcggat tagcgaaggg aagccaaacc ctgagctgcc aatgcagatt
    95521 ttaccggtga caatgcaaga gacggtttac ggcgaactgc actggcaaaa tagtcacgtt
    95581 tcatcatcag aaccgctgct taacagtgtt tcatcgatgc tgggacgcgg tttgtacttt
    95641 aatcaggcgc agaagcattt tcagcagtta ttgttgatgg aagaacgtgc gaccatcgcc
    95701 cgcgaattgc acgactcgct ggctcaggtg ctttcttact tacgtatcca gttgacgtta
    95761 ctgaagcgtt cgataccgga agataacgcc accgcacaaa gtatcatggc cgatttttcc
    95821 caggcgttga atgatgctta tcggcagtta cgcgagctgt tgactacttt ccgcctgacg
    95881 ctgcagcagg cggatctccc ctccgcgttg agggaaatgc tggatacgtt acaaaatcaa
    95941 accagcgcta aactgaccct cgactgccgt ctgccaaccc tggcactgga tgcgcaaatg
    96001 caggtgcatt tgttgcaaat tattcgcgaa gcggtgctga atgcgatgaa gcacgccaac
    96061 gccagcgaaa tcgccgtcag ttgcgtcacc gcgccggacg gcaatcacac ggtttatatc
    96121 cgtgataacg ggattggtat cggtgaaccg aaagaacccg aaggtcatta tggactgaat
    96181 atcatgcgcg aacgcgcgga acggctgggc gggacgctga ctttttcaca accttccggc
    96241 ggcggcacgt tagtgagtat tagctttcgc tccgcagagg gtgaggggaa ggggaacacg
    96301 ccggctgagg tgtaaataat taccctcaca ccggccctct ccctttaaag gcgagaggga
    96361 aaaacgtgcc catattgacc tgtaagcgag tgtgtcagca atctcatgat aatttacatt
    96421 aactcctttt tttctccacg attggctcgt accttgccgc tacagtgaag caagtcaagc
    96481 ctacaacaat acgcagaaac acgaggtcct cttttaatgg cgaatttctt tattgatcgc
    96541 cccatttttg cctgggtgct gacaatcctg ttgtgtctga caggtaccct ggcgattttt
    96601 tcattgcccg ttgaacaata ccccgatctc gcgccaccga atgtgcgagt gaccgctaac
    96661 tatcccggcg catcggccca gacgctggaa aacaccgtga cccaggttat cgagcaaaat
    96721 atgaccggcc tcgataatct gatgtatatg tcatctcaga gcagtggcac cggtcaggca
    96781 tctgtcactt taagttttaa agcaggcacc gatccggacg aagccgtgca gcaagtacaa
    96841 aaccagctgc aatcagccat gcgaaagtta ccgcaggcgg tgcaaaatca gggcgtgacg
    96901 gtgcgtaaaa ccggcgatac caacattctg accattgcct tcgtctctac cgatggctcg
    96961 atggataaac aggatattgc tgattatgtt gccagtaata ttcaggaccc gttaagccgc
    97021 gtgaatggcg tcggggatat cgatgcctat ggttcgcaat attccatgcg tatctggctg
    97081 gacccggcga aactcaacag tttccagatg acggctaaag atgtcactga tgccattgag
    97141 tcacagaacg cgcagattgc ggttgggcaa cttggtggta caccttccgt cgataagcag
    97201 gcgctcaacg ccaccattaa cgcccagtca ctgctgcaaa caccagaaca gttccgcgat
    97261 atcaccttgc gggtgaatca ggacggctca gaggtaaggc tgggcgatgt cgccaccgtc
    97321 gaaatggggg cggagaaata cgattatctt agccgcttca atggtaagcc agcctccggg
    97381 ttgggggtaa aactggcctc cagcgcaaac gaaatggcga cagcggagct ggtgctcaat
    97441 cgtctcgacg agctggcgca gtatttcccg cacggactgg aatacaaggt ggcgtatgaa
    97501 accacctcgt ttgttaaagc ctccattgaa gacgtggtga aaacgctgct ggaagctatc
    97561 gctctggttt tcctcgttat gtatctgttc ctgcaaaact tccgcgccac gctgataccc
    97621 actatcgccg tgccggtggt gttgatggga accttctccg tactttacgc cttcggttac
    97681 agcgtcaaca ccttaaccat gttcgcgatg gtgctggcga tcggtctgct ggtggatgac
    97741 gccatcgtgg tggtggaaaa cgtcgaacgt attatgagtg aggaaggact cactcctcgc
    97801 gaagccacac gtaaatcgat ggggcagatc cagggggcac tggtcgggat tgcgatggtg
    97861 ctttcggcgg tatttgtacc aatggccttc ttcggcggca ccaccggtgc catctatcgc
    97921 cagttctcta ttaccattgt tgcggcgatg gtgctgtcag tactggtagc gatgctcctc
    97981 actccggctc tgtgtgccac gctacttaag ccactgaaaa aaggtgagca tcacgggcaa
    98041 aaaggttttt ttgcctggtt taaccagatg tttaaccgca acgccgaacg ctacgaaaaa
    98101 ggggtggcga aaattctcca ccgtagcctg cgctggattg tgatttatgt cctgctgctt
    98161 ggcggcatgg tgttcctgtt cctgcgtttg ccgacgtcgt tcttaccgct ggaagaccgg
    98221 ggcatgttta ctacctcggt acaattgccc agcggttcaa cccaacaaca gaccctgaaa
    98281 gtcgttgagc aaatcgagaa atattacttc acccatgaaa aagacaacat catgtcggtg
    98341 tttgccaccg ttggttctgg ccctgggggt aacgggcaaa acgtggcgag aatgtttatc
    98401 cgcctgaaag actggagcga acgcgacagt aagaccggca cctcgtttgc cattatcgag
    98461 cgtgcaacga aggcgtttaa ccaaattaaa gaagctcgcg ttatcgccag cagcccacca
    98521 gcaattagcg gtcttggtag ttctgcaggt tttgatatgg agttgcagga ccacgctgga
    98581 gcgggtcacg atgcgctgat ggcagcacgt aatcagttgc tggcgctggc ggcggaaaac
    98641 ccggagctaa cccgtgtgcg ccataacggc ctcgatgaca gtccgcagtt gcagattgat
    98701 atcgaccagc gtaaagctca ggcgctgggc gttgatatcg acgatattaa cgacacactg
    98761 caaaccgcct ggggttcgag ctatgtgaat gactttatgg atcgcggtcg cgtgaagaaa
    98821 gtctatgtgc aggcagctgc gccgtatcgc atgctgccag atgacatcaa tctctggtat
    98881 gtccgaaata aagatggcgg catggtgccc ttctctgctt tcgcgacctc acgctgggaa
    98941 acaggctcgc cgcgtctgga acgctataac ggttattctg cggttgagat tgttggggaa
    99001 gccgcaccgg gggtcagtac cggtacggcg atggatatta tggaatcgtt agtgaagcag
    99061 ctgccaaacg gctttggtct ggagtggacg gcgatgtcgt atcaggagcg gctttccggc
    99121 gcgcaggctc cggcgctgta cgccatttcc ttgctggtgg tattcctgtg tctggcggca
    99181 ttgtatgaaa gctggtcggt accgttctcg gtaatgctgg tcgtgccgct gggggtaatc
    99241 ggcgcgctgc tggcaacctg gatgcgcggg ctggaaaacg acgtttactt ccaggtgggc
    99301 ctgttaacgg tcattggttt atcggcgaaa aacgccatcc tgatcgtcga gtttgctaac
    99361 gagatgaacc aaaaaggcca tgacctgttt gaagcgacgc tccacgcctg ccgtcagcgt
    99421 ttacgcccga ttctgatgac ctcgctggca tttatcttcg gcgtattgcc aatggcaacc
    99481 agcacgggtg ccggttccgg tggtcagcat gcggtgggta ctggcgtaat gggcgggatg
    99541 atttcagcta ctattctggc tatttacttc gtgccgctgt tctttgtgct ggtgcgccgc
    99601 cgcttcccgc tgaagccgcg cccggaataa gcaataaaaa aggcgacatg ccaatgtgtc
    99661 gcctttttca actttccgat taagaacctg ctcagcgggt tcttgctgtt tatactttgt
    99721 ctcaggaatt acttacgaag cataacttcg ataaagtctt tccagttccc cagttcacgt
    99781 tcaatcataa caacctctct tataattatg ggtattctac ggaaacaata taccgtggtg
    99841 aagctaattt actcgattgc tgcgatgact acctccgggg gacaaacctt atgtaaatac
    99901 tatggtcctg cggtgataat ttgtatgtga tacacagcaa catttcgaga tattcatacg
    99961 gcatctaata ctgatttaat tctggttaaa atacagacag ataacaagat gaatattctt
   100021 aatgtttacg ttaaaaatgt ttaatattat ttaatagttg ttaatttgaa tacttcgata
   100081 atgttatatt ttctgataat catttgcagg caaaatgttt tcacccttaa atgagtattt
   100141 attctcataa atcgaaaaag gattcattat ggttacactt tacggcatca aaaattgtga
   100201 caccattaaa aaggctcgcc gttggctgga agccaataac atcgactatc gttttcatga
   100261 ttaccgcgtc gatgggctgg acagcgaatt attgaacggt tttatcaacg aattaggctg
   100321 ggaagcgtta ctcaacaccc gtggtacaac ctggcgtaaa ctggacgaaa ctacccgcaa
   100381 taaaatcacc gatgcggcct ctgcggcggc attaatgact gaaatgcctg caattatcaa
   100441 acgtccattg ctctgcgcgc ctggtaagcc tatgctgctg ggtttcagtg attccagtta
   100501 tcagcaattt ttccatgagg tgtagtctat gtcgtgcccg gttattgagc tgacacaaca
   100561 gcttattcgc cgcccttccc tgagtcctga tgacgcagga tgtcaggctt tgttgattga
   100621 acgtttgcag gcgatcggtt ttaccgttga acgcatggat tttgccgata cgcagaattt
   100681 ttgggcatgg cgtgggcaag gtgaaacgtt agcctttgcc gggcataccg acgtggtgcc
   100741 gcctggcgac gccgatcgtt ggatcaatcc gccgtttgaa cccaccattc gtgacggcat
   100801 gttattcggg cgcggtgcgg cagatatgaa aggctcgctg gcggcgatgg tggtagcagc
   100861 agaacgtttt gtcgcacaac atcccaacca tacggggcga ctggcatttc tgatcacttc
   100921 tgatgaagaa gccagtgccc acaacggtac ggtaaaagtc gtcgaggcgt taatggcacg
   100981 taatgagcgt ctcgattact gcctggtcgg cgaaccgtcg agtatcgaag tggtaggtga
   101041 tgtggtgaaa aatggtcgtc gcggatcgtt aacctgcaac ctcaccattc atggcgttca
   101101 ggggcatgtt gcctacccac atctggctga caatccggta catcgcgcag cacctttcct
   101161 taatgaatta gtggctattg agtgggatca gggcaatgaa ttcttcccgg cgaccagtat
   101221 gcagattgcc aatattcagg cgggaacggg cagtaacaat gttattccgg gtgaactgtt
   101281 tgtgcagttt aacttccgct tcagcaccga actgactgat gagatgatca aagcgcaggt
   101341 acttgccctg cttgaaaaac atcaactgcg ctatacggtg gattggtggc tttccgggca
   101401 gccatttttg accgcgcgcg gtaaactggt ggatgcggtc gttaacgcgg ttgagcacta
   101461 taatgaaatt aaaccgcagc tactgaccac aggcggaacg tccgacgggc gctttattgc
   101521 tcgcatgggg gcgcaggtgg tggaactcgg gccggtcaat gccactattc ataaaattaa
   101581 tgaatgtgtg aacgctgccg acctgcagct acttgcccgt atgtatcaac gtatcatgga
   101641 acagctcgtc gcgtgatgag tggttctgca agaggaaata agcatggact ggctggctaa
   101701 atattggtgg attctggtga ttgtcttttt ggtaggcgtc ctgctgaacg tgattaaaga
   101761 tctcaagcgc gtcgaccata agaaatttct cgccaacaag ccggagcttc ccccgcatcg
   101821 tgatttcaac gataagtggg acgatgacga cgactggccg aaaaaggatc aaccgaagaa
   101881 gtaagctgag tgctcaaccg taggccggat aaggcgttaa cgccgcatcc ggcaatggtg
   101941 aacgatccct gatgcgacgc tcacgcgtct tatcaggcca ggtaatctgt cttttacatc
   102001 atttcaatca catcatcatc gccaggctta ccgccactta gcgcttggtc gaaataatgt
   102061 ttcggaatgg tataacgcaa gtgatccagg gcaaattgca tactgcgatt gtcgatcgca
   102121 tggcctaaat cctccacgat atccagcgtc acgtcaccac cggcactgat taacgcttcc
   102181 tgcgccgcaa ctgcatgcgc cagatcgata accggatctt caccaccatg aatcaagtga
   102241 attgtcgtgg ctgtagacgc ggtctccggc aggctcgcat agcgtccgtt aaaggcaatg
   102301 acgcgcgaag caaggcccgg ttcggcttta atgctctcca gcgccataat cgcgccttgt
   102361 gagaaaccaa ttagcgccgt ggcgttagcc cctactccgc tctgtttctg ccagtagcgt
   102421 accgtttcaa taaacgtcgg catgatggca tccacacgcg cctggcgatt atcttccgta
   102481 ataccctgca ctgaaaacca ctgacgcccc gccggattac cgctcggctc cgcgccaccg
   102541 acactcacca ccagcgcatc aggaaacagc ggtgcaaacc agctgcctat ttcccccatt
   102601 gccaccgggt tatccccgac accgtggaaa agcagtaaca actgttgtgc aggtttatcc
   102661 gggctttgaa caacaaaatg gtcatgtttc atggcgaact ccttaactga tgtcattaat
   102721 tttacgccgc tgactcacaa tgaccatggg agaaaattga aggagtcagt gaaaaaattg
   102781 ccattgcgtt atgcgatcgc gcagacgctc agtgcgaacc tcatttagtg cgaacagcgc
   102841 ctgcgcggcc tcttcccgct ggcgaaccag taacatcttg cggcctgaaa gtttaagtgt
   102901 ggtacataac tgcgcgtcac tggcgttttt ctgtaaacgc ccacgcagcg ccggtaatgc
   102961 cagttcactg gtttgtagca gacgcaataa gcaacctaac gatgttaata gcggacgatg
   103021 agcgaaagca aaaccggcca gttcaagcca gtcgtcgtca gaaaggatgg catcgtttag
   103081 tggatcaaca ggaagcattt cgccattcca ctgcgcgaga gcttgcgcat cgcgacgtaa
   103141 acggtaatgc tcacgttcag ccagctgttt acccgcatca ctcatcggta acagcgccat
   103201 cgccgtatag caaccgctgc tggcttcccg atgattaccc atccgcacca gcacaaaacc
   103261 gcagcgttgc cagaaacgcc ataactcccc agtgtaacca aaactcaccg aaagatagtc
   103321 gaggtcatgc gtatattgca aagctccagc aataagttgt cgccctgtgc cttcccgctg
   103381 acgagccgga tgaactgcta tccggctaac ccgccttccg cgcaatgtcg ccgccagtgg
   103441 attgctgccg tgcgccgcca gcgactgggc caccagatta ccccgcgggc gacgaaaacc
   103501 tgcccatacc gcctgactga gttcttgaga taatccgccc tcatccacca gccacaacgc
   103561 cccggcaatc tcgttttcgc cagccgcctg taaaaaatgt tgccctggtg catccatcat
   103621 ccggcgtaaa tccagcggcg aggtccggta gtgcgcacca gacaataact gataaacctt
   103681 taacggcgtt tctggctcgc ttcgccataa cgtttgttca aatgcggaaa tgacgatatt
   103741 gccttgtggt gtatgggtga agttttcatc gttaaaaacc agtgcctcac taaccatttt
   103801 ttccagcggg catccctgtg cccagcggat cggctgttgc agttcaaaac ggtgtaaatg
   103861 cggaaagcga gcgcaaaatt tcagcaaaaa accacgtccg gtaccttcgt agccctgcac
   103921 cgtagtggtt aacaacgttc gaggaaaacg cgataccagt tgatgcaaca acggcgcagg
   103981 tatggctgcg gcttcgtcga ccaccagcca gtcggcttgc tcatcgctgg ctaacaaggc
   104041 atccggtgca ataaagcgaa acttctcgcc cgcaaattgt gccagtacat ccgttgccgc
   104101 ttttgcgggc gcggtgacaa tcgcactgcc cgcaatacga gaaatgagtt gccctgccaa
   104161 cgccgactta ccgcgcccac gcgcagccgt taccgctgcc acgcccggcg gcatggtcag
   104221 tagctgctgt aagagttgct gttgttctgg ttgtggtgcg ccggtcgcgg ggtgccagtc
   104281 agtacgggga gtaaaatgcg ccaacgagaa cggctggttt tgccgccaga ggatagcgtc
   104341 gttatccgcc gtaagtacgc gtttgagatg ctggacaaaa tgcggcgtcg caatagggtc
   104401 agggcaatca ctccagcgca gcgagtcggc atcaggttgg ttttcccact cttcccatac
   104461 agggagtaac aaaaccagcc agcttcccgc tttcaacgtt ccgctaagtg ctgcaaaggc
   104521 agcggcatca aagccgtggc gggcgtcgaa taccgcatgc cggaactcgc gcccaagtaa
   104581 agtttgtagc gccgagggtg aacagtggtt ttcagcatct agctgcggcg aaatccacag
   104641 ccagtcgcca ggtaaggcat cacgcaactt aagagcatga tcaaaacacc aaccctcttc
   104701 cccgctcaac accagcaagc ggcagatccc ttcacgtttc atttgcgctg ttaatgtgta
   104761 aagcgcagtc agttcagcca tccctgcccc ggaaattaaa tgcttttacc aaaagtattg
   104821 cattgtgccg gatcgccgct gtcgaaacca cgtttaaacc agctgtagcg ttgctgagaa
   104881 gtgccatgtg tgaaactgtc tggtactact cgcccctgac tttgctgttg taaacggtca
   104941 tcgccgatgg cctgcgccgc attcagcgcc tcttccagat cgccagtttc cagaacgcct
   105001 tgctgctgca tactatgccc ccagacaccg gcaaaacagt cggcctggag ttccatacgc
   105061 acagataagc ggtttacttc cgcctgcgtc gcgttttgtt gcagttgacg aactttcggc
   105121 tcgatgccta acagtttctg cacatgatga ccgacttcat gggcgataac gtacccctgg
   105181 gcaaaatcgc catccgcgcc aagtttgtct ttcatgtcat catagaagga gagatcgata
   105241 taaactgtgc catccgccgg gcaatagaac ggccccataa tggactggcc cgccccgcag
   105301 ccggtgcgcg tcattccacg gtacatgacc agcttcggtt gctgataggt cttacccatc
   105361 ttttcgaact gttgtcccca ggtgtcttcc gtggttgcct gaatcaccga ggtgaatttt
   105421 gcggcttcgt cttcattagg gctaattgac cgcgttgatt gttgttgaga aaccggctgc
   105481 ccggtcatca acccggttaa atcaacgcca tagtagcctg caaccagcac gactatcagt
   105541 aaaatcagcc cgcctttacc gctcggcaga cggaaaccgg gaccgcccat tgacggaccg
   105601 ccagagctgt tgcgcctgtc ttcaacattg tcactttcac gtcgcccttg ccaacgcata
   105661 gatacctcaa caatatattc attatagaaa tgatcgtagg tggttcagag gaagattacc
   105721 acaggaaagc gggatgaaat ggcggaggaa tgagcgttca atgcatatat tccaagagcg
   105781 gttgtcttgc cgtgccagct gcacggcaag atgatgaaca gaaaaatcag tccagctgta
   105841 cacccaggcg gcgggcgacg gcttcatagg cttcgatcag gccaccgagg ctctggcgga
   105901 aacggtcttt gtccattttc tccagtgttt ctttgtccca caggcggctg ccgtccgggg
   105961 agaactcatc gcccagtacc acttcacctt tgtacagacc aaactccagc ttgaagtcga
   106021 ccagaatcag accagcatca tcgaacagtt ttttcagcac gtcgttcgct ttgtaggtca
   106081 gctctttcat acgcgccagg ttctctttgc tcacccagcc aaaggtttcg cagtaagatt
   106141 cgttgaccat cggatcgtgc atggcatcgt ttttcaggaa cagatcgaac agcggcgggt
   106201 tcagctcaat accttcttcg attccaagac gtttcactag agagccagca gcacggttac
   106261 gcacgacaca ctcaaccggc accatatcca gctttttcac cagacattcg gtatcggaga
   106321 gcagacgctc catttgagtc gggatacccg cttcagccag tttgctcata atgaagtagt
   106381 tgaacttgtt gttcaccata cctttgcgat caaactgctc aatgcgcgcg ccatcccctg
   106441 ctgacgtatc attgcggaat tcgagcacca acaggtccgg gttttccgtg ctgtatacgg
   106501 ttttcgcttt accacgatac aactcagctt gcttttgcat ctttatcact cctgggtgtg
   106561 aattaacgtt ttaaaatctt ttgctgtctg gtgtgccgga tgttttgtcg gatgcggcgt
   106621 gaacgcctta tccgaactac gtccgacaaa caaatttcgt gcgaattacc gctaaaatcg
   106681 cgttttcctg ccgacgcaca cgtttgcgta tcatatcaga aaaaagggcc ggatgattcc
   106741 agccctgtat ttttacttgc taaacgcagc ctggaagaca gctaccagcg cgtcgttctg
   106801 actctgagtc agagtatgac ctttcggatc gatgaactgt aggctgctgc ggttatctaa
   106861 atcgccaacc tgcagtttat agtcaccgga tgccaggcct ggatcgctcg cgcccagttc
   106921 ctgccagtcg ctgtcagaca gcggcttata agttacggcc atgttgccct gcgaacgggt
   106981 gctgtcggtc actttcatgc ccactttttc cagcgccgct ggcagacgtt gccaaaccac
   107041 attgaacggc ccgcgtacga ccagcattgg taaaccggtg tcatcagctg cactttgtac
   107101 gtccatagtg gtggaggcac gattttgcgc agcgttcgcg gcgtcagtgg cagatttatc
   107161 cagaccggcg gaaataacgt tcatcatctc cgtgctgtaa cgctgcatgg aagccgcgtc
   107221 tgcaaccggt ttgcccgcct gttccaggtt cagcagttta accgtaaccg cctgctgata
   107281 accctgcggc ttaacagaga tttgataacg accacgatac tgctcgtctt cgtccagacg
   107341 gttccattgt acccaatcgg tggtcagtgt ctgaccagca tcatcacgtt gggtgatggt
   107401 gtagtttttc gcctgcagca cgctaaccac ctgcggccac agagtattgc cacgaccatt
   107461 ttccaccagc aatgaagcgg tatcgcccgt gaactgggta cgcgcgccag aaaccagtgc
   107521 caacggctgg gctggtggac gaatgtccag cgccttaccg acagcaccac taccgttggt
   107581 caccgggatt gcataatcac cggaggtcac cggcaaaatc attccagccg gggcatgaag
   107641 ctccgcaagc ggtgccgctt ccaggtaggc ttcatcacca ctgacctgac gcttatagcg
   107701 tgagtcagaa ctacaggcag cgagtaataa aacaagcgaa acacccgcaa cctttgccag
   107761 gcgcgacttt tgaacagagt aagccatcaa atctccctaa actttacagc aaaccggcat
   107821 gcttaagcgc cgctctgacc gtctcacgac cactgtcggt gattggtgtc attggcaggc
   107881 gcagcgtatc ggtcgccaca agacccagtt ccttacatgc ccatttcacc gggattggat
   107941 tgggttcgac aaatagtttg ttgtgtaatg gcatcagacg ctgattaata acgcgtgcct
   108001 cggcaaaatg cccttctgct gccagtttgc acatctgggc catatcacgc gctgcgacgt
   108061 tagccgtaac ggaaataact ccatgaccgc ccaattgcat gaagtccagc gcgctcgcat
   108121 catcgccgct cagcagaaca aaatcatctg aaaccagctc tttgatctgg tttacacgcg
   108181 ttaagttccc tgttgcctct ttgattccga taatattttt tactttcgcc agacggccca
   108241 ccgtttccgg gagcagatcg cagccagtac gggacggcac attatacaga atttgcggca
   108301 ggtcagtatg ctcagcgatg gctttgaaat gctgatacaa accttcttgc gacggacgat
   108361 tgtagtaagg ggttaccgtc aggcagccga cgataccact gtcattgaag cgctgcgtca
   108421 ggctaatggc ttccgcagta gcgttagcgc cggtcccggc aattaccgga atgcgcccat
   108481 cagccagatc cagcgtcatc atcaccacat cagcatgttc gtcatgattt aaggtagcgg
   108541 actcgccagt ggtgccaaca gaaacgatcg ccgaagtacc gctggcgaca tgataatcaa
   108601 tcagtttttt caagctagcc cgacagacat tacctttttc atccatcgga gtaacaatcg
   108661 cgacaatact tcccgtgaac atgggccatc ctctgtgcaa acaagtgtct caatggtacg
   108721 tttggtatgg cattaaaagc aagcagacag aaccgttctg attgttgtat gcatgatttt
   108781 tttatgcttt ccttaagaac aactcacccc ttaaaggaat aaccagtttg acactgtcat
   108841 cgcaacatta tctggtgatc actgcgttgg gtgccgatcg ccctggaatt gtgaacacca
   108901 tcacccgtca tgtcagtagt tgcggctgta atattgaaga cagtcgcctg gcgatgctgg
   108961 gagaagagtt cacgtttatc atgctgcttt ccggttcatg gaatgccatc actctgattg
   109021 aatcaacgtt accgttgaaa ggtgccgaac tggatctttt aatcgtgatg aagcgcacga
   109081 cggcgcgtcc gcgtccgcca atgccagcat ctgtctgggt tcaggtcgat gttgcagact
   109141 ccccgcattt aattgaacgc ttcacagcac ttttcgacgc gcatcatatg aacattgcgg
   109201 agctggtgtc gcgcacacaa cctgctgaaa atgaacgggc tgcgcagttg catattcaga
   109261 taaccgccca cagccccgca tctgcggacg cagcaaatat tgagcaagcg ttcaaagccc
   109321 tatgtacaga actcaatgca caaggcagta ttaacgtcgt caattattcc caacatgatg
   109381 aacaggatgg agttaagtaa tgaatccact gaaagccggt gatatcgcac cgaaatttag
   109441 cttgccggat caagacggag aacaagttaa tttgaccgac ttccagggac agcgtgttct
   109501 ggtttatttc tacccgaaag ccatgacccc cggctgtacc gtacaggcct gcggcttacg
   109561 cgataacatg gatgagttga aaaaagcggg cgttgatgtg ctgggtatca gcaccgataa
   109621 acccgaaaaa ctctcccgtt ttgcggaaaa agagctgctt aactttacgc tcctgtctga
   109681 tgaggaccac caggtgtgcg aacaattcgg cgtctggggt gaaaagtcct tcatgggcaa
   109741 aacctacgat ggtattcatc gcatcagctt cctgattgac gctgatggca aaatcgaaca
   109801 tgtctttgac gatttcaaaa ccagcaatca ccacgacgtt gtgctgaact ggctgaaaga
   109861 acacgcctga ttactttgct ccattccgtg ctggctgcgc ttgcggccag catacctcac
   109921 ttctcgtgat caagatcaca ttctcgcttt cccctgcgac acgggtgtcg aatccatttt
   109981 ttgctgaacg ttaatgacca tcatttttgt accgttcaga atccagttaa tacataactt
   110041 attgaataga ttgagttaat cagaatggca tcctttatac aatatgaaat gcaatgtttc
   110101 atatcatttt caaggagccg acatgaaccg ctttgtggtg gccgaaccac tgtggtgtac
   110161 aggatgtaat acctgtctcg ctgcctgttc ggacgtgcat aaaacgcaag gtttacagca
   110221 acacccgcgc ctggccctgg cgaagacgtc aacaatcact gcccctgtcg tgtgtcatca
   110281 ctgtgaggaa gccccttgcc tgcaggtctg cccggtcaat gccatctctc agagggatga
   110341 tgcgatccaa ctcaacgaaa gcctctgtat tggctgcaag ctttgcgccg tggtctgccc
   110401 atttggcgca atcagcgctt caggaagccg tccggtgaat gcccatgcgc aatatgtttt
   110461 tcaggctgaa ggctcactca aagacggcga agaaaacgtg ccgccccaac atgcgttgct
   110521 gcgctgggaa ccgggtgtcc agaccgtcgc ggtgaaatgc gacctgtgtg atttcttgcc
   110581 agaaggtccg gcctgcgttc gcgcttgccc gaatcaggcg ttacggctga tcaccgacga
   110641 tagcctgcaa cgtcagatga aagaaaaaca gcgccttgcc gcaagctggt ttgccaatgg
   110701 cggggagtct cccctttccc tcactcagga gcaacactaa tggatgccct gcaattatta
   110761 acctggtcgc tgatcctcta tctgtttgcc agtctggctt cgctgttttt actcggtctg
   110821 gacagactgg ctattaagct ttccggcatc acatcgctgg tgggcggcgt gattggcatc
   110881 atcagcggaa ttacgcaatt acatgctggt gtaactttag tcgcccattt tgccacccct
   110941 tttgactttg ccgatttaac cctgcgaatg gatagcctct cggcatttat ggtgctagtt
   111001 atctccttgc tggtggtggt ttgttcgctc tattcattga cttatatgcg cgaatacgag
   111061 ggcaaaggcg cggcggcgat gggcttcttt atgaatcttt tcatcgcatc gatggttgcc
   111121 ctgctggtga tggacaacgc tttttggttc atcgtgctgt ttgaaatgat gtcgctgtct
   111181 tcctggtttc tggtcattgc caggcaggat aaaacgtcga tcaacgctgg catgctctac
   111241 ttttttatcg cccacgccgg atcggtgctg attatgatcg ccttcttgct gatggggcgc
   111301 gaaagcggca gcctcgattt tgccagtttc gcacgctttc actttctccg gggctggcgt
   111361 cggcggtgtt tctgctggcc tttttcggtt ttggcgcgaa agccgggatg atgccgttgc
   111421 acagctggtt gccgcgcgct caccctgccg caccatcgca cgcttcggcg ttgatgtctg
   111481 gcgtaatggt caaaatcggt attttcggca tcctgaaagt agcgatggat ctgctggcgc
   111541 aaacgggttt gcctctgtgg tggggcattc tggtgatggc gatcggcgca atctccgcgc
   111601 tcctgggcgt gctatatgcg ctggcggaac aggatatcaa acggctgctg gcctggagta
   111661 ccgtcgaaaa cgtcggcatt attttgctgg cagtcggtgt ggcgatggac ggtctgtcac
   111721 tgcacgaccc gctgctcacc gtggttggac tgctcggcgc actgtttcat ctgctcaacc
   111781 atgcgctgtt caaagggctg ctatttctcg gcgcggacgc gattatttcg cgtttgcata
   111841 cccacgacat ggaaaaaatg ggggcactgg cgaaacggat gccgtggaca gccgcagcat
   111901 gcctgattgg ttgcctcgcg atatcagcca ttcctccgct gaatggtttt atcagcgaat
   111961 ggtacacctg gcagtcgctg ttctcactaa gtcgtgtgga agccgtagcg ctacaacttg
   112021 cgggtcctat tgctatggta atgctggcag tcactggtgg gctggcagta atgtgcttcg
   112081 tcaaaatgta cggtattact ttctgtggtg cgccgcgcag tacacacgct gaagaggcac
   112141 aggaagtgcc aaatacgatg atcgtcgcca tgctactgct cgcggcactc tgcgtattaa
   112201 ttgcgcttag tgccagttgg ctggcaccga agataatgca tattgcccat gcgtttacca
   112261 atacccctcc cgtcactgtc gccagcggaa tagcacttgt acccggcacg tttcatacac
   112321 gggtcactcc ttcattactg ttgctgttac tactggcgat gcctttgctg ccaggccttt
   112381 actggctgtg gtgtcgttcg cgccgcgcag cgtttcgtcg cacaggagat gcctgggcat
   112441 gcggctacgg ctgggaaaac gcgatggctc cgtcaggcaa tggcgtgatg cagccgctgc
   112501 gtgtggtctt ctctgcgtta tttcgtctac gacaacagct cgaccctacg ctgaggctaa
   112561 ataaaggtct tgcgcacgtc accgccaggg ctcagagcac agaacccttc tgggatgagc
   112621 gggtgatccg ccccatcgtg agcgccaccc aacggctggt caaagaaata cagcatctgc
   112681 aaagcggcga ctttcgtctc tattgcctgt atgtggtcgc cgcactggtt gtgctgctaa
   112741 tcgctattgc cgtctaagga aatcaccatg agacaaactc tttgcgacgg atatctggtc
   112801 atttttgcgt tagcacaggc cgtgattctg ctgatgctaa ccccactttt tacgggtatt
   112861 tcccggcaga tacgcgcgcg tatgcactcc cgccgcgggc cggggatctg gcaggattat
   112921 cgcgatatcc acaaactgtt taaacgccag gaagttgcgc cgacatcttc aggtctgatg
   112981 ttccgcctga tgccgtgggt attaatcagc agcatgctgg tgctggcgat ggccttacca
   113041 ctatttatta ccgtttcccc ttttgcgggc ggcggcgatc tgatcaccct tatctatctt
   113101 cttgccctgt ttcgtttttt ctttgctctt tccgggctgg ataccggaag tccgtttgcg
   113161 ggagtcggtg ccagtcgcga gttgacgctc ggcattctgg tcgaaccaat gcttattctc
   113221 tcactgctgg tattggcgct gatagcaggt tccacgcata tcgagatgat cagcaatacg
   113281 ctggctatgg gctggaactc gccgctaacc accgtactgg cgttactggc ctgtggtttt
   113341 gcctgcttca ttgagatggg aaaaattccc tttgatgttg ctgaagcaga acaggaatta
   113401 caggaaggcc cgctgaccga atattccggt gccgggctgg cgctagcgaa atgggggctg
   113461 gggctgaaac aggtcgtgat ggcagcactg tttgtggccc tgtttctgcc ctttgggcgc
   113521 gcgcaagaac tttctctcgc ctgcctgctg acttcacttg tcgttacgct gctcaaggtt
   113581 ttgctgattt ttgtactggc ctcaatcgca gaaaacacgc tggcacgcgg gcgtttttta
   113641 ctcattcacc atgtgacctg gcttggcttc agccttgctg cgcttgcatg ggtcttttgg
   113701 ttaaccggtc tgtaaggagc actgacggaa tatggaaaat cttgctctga cgacgttaat
   113761 gctgcctttt atcggcgcac tggtcgtttc gttttcgcca caacgtcggg ccgccgaatg
   113821 gggggttttg ttcgccgcgc tgaccacgct gtgcatgttg tcgctgatct ccgcgtttta
   113881 tcaggccgat aaagttgccg tcacgttgac gttggtcaac gtgggggatg tggcattatt
   113941 tggcctggtc attgatcgcg tgagtacgct gattctgttt gtggtggtgt ttctcggttt
   114001 gctggtcacg atctactcca cgggttatct gacggataaa aatcgcgaac acccgcataa
   114061 cggcacgaat cgttattacg catttttgct ggtgtttatc ggcgcgatgg cgggactggt
   114121 actctcctcg acgctgctcg gtcagttgtt gttttttgaa attacgggcg gctgctcctg
   114181 ggcgttgatc agttattacc agagcgataa agcgcagcgt tcagcactaa aagcgttgct
   114241 tatcacccat atcggttcgt tggggttgta tcttgccgcc gccacgctgt ttttgcagac
   114301 cggaacgttt gtgcttagcg cgatgagcga gttacatggc gacgcacgtt atctggttta
   114361 tggcggcatc ctgtttgccg cgtgggggaa atcggcccag ctaccgatgc aagcgtggct
   114421 accggacgca atggaagcgc caacaccgat cagcgcctat ctccacgccg catcgatggt
   114481 gaaagtgggc gtttacattt ttgcccgcgc cattatcgac ggcggcaata tcccgcatgt
   114541 gattggcggc gttggcatgg tcatgacact ggtcaccatt ctttatggct ttctgatgta
   114601 tttgccacag caggatatga agcggttgct ggcctggtcg accatcactc aacttggctg
   114661 gatgttcttc ggcttgtcgc tctccatctt cggctcgcgg ctggcgctgg agggtagcat
   114721 cgcctacatc gtcaaccacg cgttcgctaa aagcctgttt ttccttgtag caggtgcgct
   114781 gagttacagc tgcggcacgc gcttgttgcc gcgtctgcgt ggcgtattgc acaccctgcc
   114841 gttgccaggc gtgggtttct gcgtggcagc gctggcaatt accggtgtgc cgccgttcaa
   114901 cggcttcttc agtaaattcc cgctgtttgc cgccggtttt gcgttgtcag tggagtactg
   114961 gatcctgctg cccgccatga ttctgctgat gattgaatcg gtcgccagtt tcgcctggtt
   115021 tattcgctgg tttggtcgcg tcgtgccggg caaaccgagc gaggccgtcg ccgatgccgc
   115081 accgctgcca gggtcaatgc gcctggtgtt gattgtactg attgtgatgt cgctgatttc
   115141 cagcgtaatc gccgcgacct ggttgcagta aggagatgat gaatgaccgg ttctatgatc
   115201 gtaaataatc tggcgggact gatgatgctg acatcgctgt ttgtgattag cgtcaaaagc
   115261 tatcgcctgt catgcggatt ttacgcctgc cagtcgctgg tgctggtgtc tattttcgcc
   115321 actctctcgt gcctgttcgc cacagagcaa ctgctgatct ggtccgccag cgcctttatc
   115381 accaaagtgc tgctggtacc gttaatcatg acttacgctg cacgaaatat tccccagaac
   115441 atcccggaaa aagcgttatt cggtccggca atgatggcac tgctcgcggc gttaattgtc
   115501 ctgctttgcg catttgtcgt tcagcccgtg aagctaccga tggctaccgg gctgaaaccg
   115561 gcgctggcgg tagcgttagg tcattttctg cttggcctgc tgtgcattgt cagccaacgc
   115621 aatatcctgc ggcaaatttt tggttactgc ctgatggaaa acggctccca tctggtgctg
   115681 gcgcttcttg cctggcgagc accggaactg gtggaaatag gtatcgctac cgacgccatc
   115741 ttcgccgtca ttgtgatggt gttactggca agaaaaatat ggcgtaccca cggcacgctg
   115801 gacgtgaaca acttgaccgc gctgaaggga taatgagatg agttattctg tgatgttcgc
   115861 tttactcctg ctcacgccgc tgcttttttc gctgctctgt tttgcctgcc ggaaacgggg
   115921 gctctctgcg acttgtacgg tgactgtatt acatagctta gggatcacac tgctgctgat
   115981 tctagcactc tgggtggtcc aaactgccgc tgatgcagga gaaatattcg ctgcgggact
   116041 gtggcttcat attgatggtc tgggcggttt gttcctcgcc attcttggtg tgattggctt
   116101 tctcaccggt gtttactcga ttggctacat gcgtcatgaa gtggcacacg gcgagctttc
   116161 acccgttaca ctgtgcgatt actacggttt cttccatctg tttttgttca ccatgctgct
   116221 ggttgttacc agcaataacc tgattgtgat gtgggcggcg atcgaagcca ccaccttaag
   116281 ctcggcgttt ctggtaggca tttacggtca gcgttcatcg ctggaatcag catggaagta
   116341 catcattatt tgtactgttg gtgtcgcttt tggtctgttc ggtaccgtgc tggtatacgc
   116401 caacgccgcc agcgttatgc cgcaggcaga aatggcgata ttctggagcg aggttcttaa
   116461 gcaatcgtcc ttgcttgacc caacattaat gctgttggcc tttgtgtttg tgctaattgg
   116521 ctttggtacc aaaaccgggc tatttcccat gcacgcctgg ctgccggatg ctcacagtga
   116581 agcgccgagt ccggtcagcg ccctgctctc cgccgtattg ctgaactgcg cgctgttggt
   116641 gctgattcgc tattacatca ttatttgcca ggccatcggc agcgatttcc ccaaccggtt
   116701 gttgctcatc ttcggcatgt tgtcggttgc cgtggcggca tttttcattc tggtacagcg
   116761 ggacattaag cgtctgctgg cgtactccag cgtggagaac atggggctgg tcgcggtggc
   116821 gctaggcatt ggcgggccgc tgggaatttt tgccgcgctg ctgcacacct taaaccacag
   116881 tctggcaaaa acgctgctgt tctgcggttc cggcaatgta ctgctcaagt acggcacgcg
   116941 cgatctcaac gtcgtctgtg ggatgctcaa aatcatgcca tttaccgccg tgctgtttgg
   117001 cggcggtgcg ctggcgctgg cgctggcagg gatgccgccc ttcaacattt ttcttagcga
   117061 atttatgacc gttaccgcag gactggcacg taatcacctg ctgattatcg tcctgctgtt
   117121 attgctgtta acgctggtgc tggcgggcct ggtacggatg gctgcgcggg tgttaatggc
   117181 gaaaccgccg caggccgtta accggggtga tctcggctgg ttgaccacct cgccaatggt
   117241 gattctgctg gtcatgatgc tggcgatggg aacgcatatt ccacaacctg tcatcaggat
   117301 cctggcgggc gcttccacta tagtcctctc agggacgcac gacctgcctg cacaacgtag
   117361 cacctggcat gattttttgc cttcaggcac cgcatctgtt tcggagaaac acagtgaacg
   117421 ttaattcatc gtcaaatcgt ggcgaagcga ttctcgccgc cctgaaaacg cagttccccg
   117481 gcgcggtgct ggatgaagag cgacaaacgc ctgaacaggt caccattacg gtgaaaatca
   117541 atctgctgcc tgacgttgta cattatcttt attatcaaca tgatggctgg cttccagtcc
   117601 tgtttggcaa cgacgagcgg acacttaacg gtcattacgc ggtttattat gccctttcaa
   117661 tggaaggggc cgaaaaatgc tggatcgtgg tgaaggcact ggtcgatgcc gacagtcggg
   117721 agtttccgtc agtcacaccg cgcgtccctg ccgcggtctg gggcgagcga gaaattcgcg
   117781 atatgtacgg gctgattccg gttggcctgc cggatcagcg tcgcctggtg ttgcccgatg
   117841 actggccgga agatatgcat ccgctgcgca aagatgcgat ggattatcga ctgcgccctg
   117901 aaccgacgac agattccgaa acgtatgcgt ttatcaatga gggcaacagc gatgcgcggg
   117961 tgatccctgt cggcccgctg catatcacct cctatgaacc gggtcacttc cgcttgtttg
   118021 tggatggcga gcaaattgtc gatgctgatt accgcctgtt ttatgtccat cgcggcatgg
   118081 agaaactggc agaaacgcgg atgggctaca acgaagtgac cttcttatcg gaccgcgtgt
   118141 gtgggatttg cggttttgcc cacagtgtgg cctataccaa ctcggttgaa aatgcactgg
   118201 ggattgaggt gccgcaacga gcacatacta ttcgctcgat tctgctggaa gtcgagcggc
   118261 tacacagtca tttgctcaac cttggcctct cctgccattt tgttggtttt gataccggct
   118321 ttatgcaatt tttccgcgtg cgggaaaagt cgatgacgat ggcggaattg ctgaccgggt
   118381 cgcgtaaaac ctacggtctg aatctgattg gtggtgttcg ccgcgatatt ctcaaagagc
   118441 aacgtctgca aacgctgaaa ctggtgcgcg agatgcgcgc cgacgtgtcg gagctggtag
   118501 agatgctgct tgctacgccg aatatggaac aacgcactca gggcattggc attctcgacc
   118561 gacaaatcgc ccgtgattat agccctgtag ggccgctgat ccgcgcagtg gttttgcccg
   118621 tgatttgcgc tttgatcacc cctacgccga ctacggcaat attccaaaaa cactgtttac
   118681 ctttaccggc ggcgatgttt tctcccgcgt gatggtccgt gtcaaagaga cgtttgattc
   118741 gctggcaatg ctggaatttg ccctcgacaa catgccggat accccactgc tgaccgaagg
   118801 ctttagctat aaacctcacg cattcgcgct gggctttgtt gaagcgccac gcggtgaaga
   118861 cgtgcactgg agcatgctcg gtgataacca aaaattgttc cgctggcgct gtcgtgccgc
   118921 cacctacgcc aactggccgg tgttgcgtta catgctgcgc ggcaataccg tttctgacgc
   118981 accgctgatt atcggtagcc ttgatccctg ctactcctgt accgaccgtg tgacgctggt
   119041 agatgtgcgc aagcgccagt caaaaaccgt gctgtataaa gagatcgaac gctacggcat
   119101 tgatcgtaac cgttcgccgc tgaagtaagg acagaagatg ctgaagttac tgaaaactat
   119161 tatgcgcgcc ggaaccgcga cggtgaaata tcccttcgcg ccactggagg tcagcccagg
   119221 ctttcgcggg aaaccggacc tgatgcccag ccaatgtatt gcctgcggtg cctgcgcctg
   119281 tgcttgtccg gcaaatgcgc tgactatcca gaccgacgac cagcaaaatt cgcgcacctg
   119341 gcagctctat ctggggcgtt gtatttactg cggacgttgt gaagaagtgt gcccgaccag
   119401 agccatccag cttaccaata actttgaact gaccgtcacc cataaagccg atctctatac
   119461 ccacgcgacg ttccatctac aacgttgcag ccgttgcgaa cgcccgtttg ccccgcaaaa
   119521 aaccgtcgca ctggctgctg aattgttggc acagcaacaa aatgctccac aaaaccgcga
   119581 aatgttatgg gcgcaagcga gcgtctgccc ggagtgcaaa caacgcgcaa cgctgatcaa
   119641 cgacgataca gatgtaccgc tggtggctaa ggagcagcta tgagtccagt gcttacgcaa
   119701 tatgtcagcc agcccatcac gctggacgag caaacgcaaa agatgaagcg gcatttgcta
   119761 caggatatcc gtcgctcggc ttacgtttat cgcatcgatt gcggcggctg caacgcctgt
   119821 gaaatcgaaa tttttgctgc catcaccccc gtattcgacg cagaacgttt tggcattaag
   119881 gttgtttcat caccgcgtca cgccgatatt ttgttattta ctggcgcagt cacccgggcg
   119941 atgcgtatgc ctgcacttcg ggcgtatgag tctgcccccg atcataaaat ctgtgtttcc
   120001 tacggcgcgt gcggtgtcgg cggcggtatt ttccacgatc tctacagcgt ctggggcggc
   120061 agcgacacca ttgtccccat tgatgtttgg atccccggct gcccgccaac accggccgcc
   120121 accattcacg gtttcgccgt ggcgctcggt ttgctgcaac agaagattca cgcggtggat
   120181 tatcgcgatc ccaccggggt gactatgcaa ccgttgtggc cgcagatccc gccatcacag
   120241 cgtatcgcca ttgagcgaga agcgcggcgg ctggcgggct atcgtcaggg gcgagaaatt
   120301 tgcgatcggc tcctgcgcca tttaagcgac gatcctacag gaaatcgggt taacacctgg
   120361 ttgcgcgatg ccgacgatcc acgtctcaat agtatcgttc agcaactctt tcgcgtactc
   120421 cgggggttac atgactgaag agtgcgggga aattgttttc tggacgctgc gaaaaaagtt
   120481 tgtcgccagt agcgacgaga tgccggaaca cagctctcag gtaatgtatt actcgctggc
   120541 tatcggtcat cacgttggcg tgattgattg tctgaatgtc gccttccgct gcccactgac
   120601 ggaatacgaa gattggcttg cactggtcga agaggagcaa gcccgacgta agatgctggg
   120661 ggtgatgact tttggtgaga ttgttattga cgccagccac accgccctgt tgacccgggc
   120721 attcgcgcca ctggcagatg acgcgacgtc tgtgtggcaa gcgcgtagca ttcaattcat
   120781 tcatctgttg gatgaaattg tgctggaacc ggctatctat ctgatggcca gaaaaattgc
   120841 gtgagaagga tttctcatta ataaggactg ttgatggcta tgtcagacga agcgatgttt
   120901 gccccgccgc aaggaataac aattgaagcg gtaaacggaa tgctcgcgga gcggttagcg
   120961 cagaaacacg gcaaggcgtc tttattacgc gccttcatcc cgctgccgcc gccgttcagc
   121021 ccggtacaac ttattgaact gcatgttctc aaaagcaact tctattaccg ctaccatgat
   121081 gatggcagcg atgtgacggc aacaacagag tatcagggcg agatggtcga ttattcgcgt
   121141 tacgccgtcc ttctcggcag tagtggaatg gcggagctac gctttattcg tacccacggc
   121201 agccgtttta ctccccagga ttgcacactg tttaactggc tggcacgcat tatcaccccg
   121261 gttctgcaat catggctcaa tgatgaagca cagcaggtgg cgctgcgttt gctggagaaa
   121321 gatcgcgatc atcatcgggt actggtggat atcactaatg cagtgctgtc acatcttgat
   121381 ctcgacgatc tgatcgctga cgtcgctcgt gagatccatc attttttcgg tctggcttca
   121441 gtcagtatgg tactgggcga tcatcgaaag aacgagaagt tcagcctgtg gtgcagcgat
   121501 ctttctgcct cacattgtgc gtgtctgcca cacaatatgc ctggcgacag tgtattgctg
   121561 acacaaacgc tacaaacccg acaaccgacc ttgacgcacc gtgcagatga tctgtttctc
   121621 tggcaacgcg acccgttatt actcttactt gcatctaacg gctgcgaatc tgcgctcctt
   121681 ataccgctta cctttggcaa ccatacaccg ggtgcattgt tgctggcgca tacctcttcc
   121741 actctcttta gtgaggaaaa ctgccagcta ctacaacaca tagccgatcg catcgctatt
   121801 gccgttggca atgccgatgc ctgccgtaga atgaccgatt tgcaggaaag tttgcagcaa
   121861 gaaaaccacc agcttagcga gcagctcctt tcgaatctgg gcgtcggtga cattatctat
   121921 caaagccagg caatggaaga cctgctccag caggtagata ttgtggcgaa gagcgacagt
   121981 acggtgttga tttgcggtga aaccggaacc ggcaaagagg tgatcgccag agcgatccat
   122041 caacttagcc cgcgacacga caagccgctg gtcaaaatca actgcgctgc catccccgcc
   122101 agtcttctgg aaagtgagtt attcggtcat gacaaagggg cgtttactgg tgcgattaat
   122161 acccatcgtg gtcgttttga aattgccgat ggcggcacgt tgtttctcga tgaaattggc
   122221 gatctgccgt tagaacttca gcctaaactg ctgcgcgtat tgcaggaacg ggagattgag
   122281 cgtctcggcg ggagtagaac gatcccggta aatgtcagag tcattgccgc caccaaccgt
   122341 gatttgtggc aaatggttga agatcgccag tttcgcagcg atctctttta tcgcctgaat
   122401 gtcttcccac tggaattgcc tccgctgcgc gaccgtccgg aagatatccc tcttttagca
   122461 aagcatttca cgcaaaaaat ggcgcgccat atgaatcgtt caattgacgc catcccgacc
   122521 gaggcgctac gccagttgat gtcgtgggac tggccgggca acgtgcgcga gctggaaaac
   122581 gtgattgagc gagcggtact gctgactcgc ggtaacagtc tgaatttaca tcttaatgtc
   122641 cgacaaagcc gtttactgcc gacgctaaat gaagattcag cgcttcgcag ttcaatggcg
   122701 tagttgctgc acccgacgac gccagagaat gacgaagaag aacgtcagcg cattgttcag
   122761 gtattgcgag aaaccaatgg cattgttgcc gggccccgtg gcgcggcgac acgattaggg
   122821 atgaagcgca ccacgctgct gtcacgaatg cagcgtctgg gaatctcggt tcgcgaggtg
   122881 ttgtaatctg cttttgcagg agtatgcatg agaaacaaac tctctttcga cttgcagttg
   122941 agcgccagaa aagcggcaat cgctgaacgg attgccgccc ataaaattgc ccgcagtaaa
   123001 gtgtcggtct ttttaatggc gatgtccgct ggcgtgttta tggcgatcgg atttactttt
   123061 tacctttccg ttatcgccga tgccccgtct tcataggcat taacccatct ggtgggcggc
   123121 ctttgcttta cacttggctt tattttgctg gcggtttgcg gcaccagcct gttcacctcg
   123181 tcggtaatga cggtaatggc aaaaagtcgg ggcgttatta gttggcgaac ttggctgatt
   123241 aacgcacttc tggtggcctg cggtaatctg gcaggtattg cctgtttcag tttgttaatc
   123301 tggttttccg ggctggtgat gagtgaaaac gcgatgtggg gagtcgcgat tttacactgc
   123361 gccgagggca aaatgcatca tacatttact gaatctgtca gcctcggcat tatgtgcaat
   123421 ctgatggttt gcctggcgct gtggatgagt tattgcggac gttcgttatg cgacaaaatc
   123481 gtcgccatga ttttgtccat caccctgttt gtcgccagtg gctttgagca ctgtatcgcc
   123541 aatttgtttg tgattccgtt cgccattgcc attcgccatt tcgcccctac ctccttctgg
   123601 caactggcgc acagtagcgc agacaatttt ccggcactga cggtcagcca ttttattacc
   123661 gccaatctgc tcccggtgat gctgggtaat attatcggcg gtgcagtgct ggtgagtatt
   123721 tgttatcggg ctatttattt acgtcaggaa tcctgaaaaa tcagcccggc gaaacagttc
   123781 gtcgggctga agattattct tgcgcgattt gcccatcggg ccaggcgtgg atcacggctt
   123841 tgatcagcgt cgccaatgga atggcgaaga atacgcccca gaatccccac aaaccaccga
   123901 agatcaccac cgataaaata atcaccagcg gatgcaggtt aaccgcttcg gagaacaaca
   123961 ccggtactag caggttgccg tccagcgcct gaataatcag atacaccgcg aagcagctcc
   124021 agaattcagt acctgcgcca aactggaata gcgccacgcc aaccaccgga atggtcacca
   124081 caaatgcgcc aatgtacgga ataagaaccg agaagccgac cagcactgcc agcagcagcg
   124141 aatagttcag cccgaagagc aagaacccca gccaggtggc gatccccacc acgatcatct
   124201 ccagcacttt gccgcggata tagttggtga tttgttgatt catctccttc cacacctgtc
   124261 ctgccagccc acggttacgc ggcagcaccc ggcgaacggc gttcagcatc tgctctttgt
   124321 ctttcagcag gaagaagacc atcaatggca ccagcaccag atagacggct atggtcagca
   124381 aaccgaccag cgaggcgagg gaaattttca ccaccgaatc gcccatggtc agcatccgac
   124441 tgcgcatatt ttcggccatt gcatcaataa tgcccgcgtc cattaacacc ggatagcggc
   124501 gcggcaacgt ggcggcaaag tcagaaagct tattgagcat ccccggcata tcgcggatta
   124561 agtagatgcc ctgttgccag gcgataggca gtaccacgaa cgccatcagt agcaatatac
   124621 cgacaaaaac caccaataca atcgacgtcg cccagcggcg ggagcagcca atagattgca
   124681 ggcgcacggt tggccattcc agcaaataag ccagcacaat agccaccagc aacggagcaa
   124741 gcaggccact aaagaaaaag ataatgccaa atccggcaac taaaataacc agcaaggcaa
   124801 tcgcttccgg gtcgctaaaa cggcggcgat accattgcat caacatttcg agcataaaga
   124861 ccttccctga acctcaagag cgggattgcg atccgcaatt gtatcgaaat gtcacaaaaa
   124921 agacttcgct ttttatgacg gattcaggaa actgaaaagt catttgagtg ggctaatctt
   124981 cgccgttaca ctcaaaggcg gcgaggtggg aacgatattt cacagtatcg gtcaaatgac
   125041 tatcttcaga aatacaggat agaggttatg ttcaggcagt tgaaaaaaaa cctggttgca
   125101 accctcattg ctgctatgac cattggtcag gtagccccgg cgtttgccga cagcgcagac
   125161 accttgccgg atatgggaac ctccgcagga agcacgcttt ccattggtca ggaaatgcag
   125221 atgggcgact attatgtccg ccagctacgc ggcagcgcgc cgttaattaa tgacccgctg
   125281 ttaacgcaat atattaattc gctggggatg cgtctggttt cgcatgccaa ttcggttaag
   125341 acaccgtttc atttttttct gatcaacaac gacgaaatta acgcctttgc tttctttggc
   125401 ggcaacgtgg tgctgcactc tgccctgttc cgttattccg ataacgaaag tcaactggct
   125461 tcagttatgg cgcacgaaat ctcccacgtc acccaacgtc acctggcgcg agcgatggaa
   125521 gatcaacaac gcagcgcgcc gctgacctgg gtcggcgcgt taggttctat tttactggcg
   125581 atggccagtc cgcaggcggg gatggcggcg ctgaccggta cactggcggg aacgcgccag
   125641 gggatgatca gtttcaccca gcaaaatgaa caggaagcgg accgcattgg tattcaggtg
   125701 ctgcaacgct cgggattcga tccacaggcg atgccgacct tcctggaaaa attgctcgat
   125761 caggcgcgtt actcctcgcg cccgccggaa attttattga ctcacccgtt gccggaaagt
   125821 cgtctggcag atgcccgcaa ccgtgctaat cagatgcgcc cgatggtggt gcagtcgtcg
   125881 gaagatttct atctggcgaa agcgcgcaca ctggggatgt ataattccgg acgtaaccag
   125941 ctcaccagtg atttgctgga tgaatgggcg aaaggaaacg ttcgtcagca acgagcggcg
   126001 caatatggtc gtgctttaca ggcgatggaa gccaataaat acgacgaggc gcgtaaaacg
   126061 ctgcaaccgt tactggcggc agaacctggc aacgcatggt atctcgatct ggctaccgat
   126121 atcgatcttg ggcaaaacaa agccaatgag gcaatcaatc gcctgaaaaa tgcccgcgat
   126181 ttacgcacca atccggtgtt gcagctcaac ctggcgaacg cttatctgca aggcggtcaa
   126241 ccacaagaag cggccaatat tctgaatcgc tacaccttta ataataaaga tgacagcaac
   126301 ggctgggatt tgctggcaca ggcggaagcc gcgctaaata accgcgatca ggagctggct
   126361 gcgcgagcag aaggttatgc gctcgccgga cgactcgatc aggccatttc gctgttgagt
   126421 agcgccagtt cgcaggtgaa attaggcagc ctgcaacaag cgcgttacga tgcgcgcatc
   126481 gaccagttgc gccagctgca gaaacgcttt aagccttata ccaagatgta attcgcatta
   126541 aaggaagaac aatgaccaaa caggtaaaaa tctaccataa cccacgctgt tcaaagagcc
   126601 gggaaacgct gaacctgctg aaagaaaacg gcgtggagcc agaagtggtg ctctaccttg
   126661 agacacccgc cgatgcggca acgctgcgcg atttgctgaa aatgctgggg atgaatagcg
   126721 cccgtgaatt gatgcgccag aaagaggatc tttataaaga actaaacctg gctgacagtt
   126781 cgcttagcga agaggcgctg attcaggcaa tggtggataa tccgaagctg atggagcgcc
   126841 cgattatggt cgcgaatggc aaagcgcgga ttggtcgtcc accggagcag gtactggaaa
   126901 tcgtgggtta agatgtgctc catcgctagt tgaagcacat tgccggatgc gacgcttgcc
   126961 acgtcttatc cggcctacga atactatgga ttaacttcgc gtaggtcagg cgttcgcgcc
   127021 gcatccgaca ataaacacct tatctacaac ttcagaattt ctttcacaaa cggaatggtc
   127081 agcttacgtt gcgcggtaat cgacgcacga tccaactgat ccaacgtcat aaatagcgtg
   127141 cgcatttctc tgtcgagccg cttcagcaag aaacgcccca catcttccgg cagttcaaaa
   127201 ccacgcaaac gcgcgcgtaa ctgtagcgcc tgcaacttat cttcatcaga aagtggctgc
   127261 aatttgtaga tctgccccca gtcgagacgc gacgcgagat ccggtaatcc cagattcaac
   127321 tgccgcggtg gacgatcgcc ggtgatcaac aaccgtgttt tgcccgattc cagaattcga
   127381 ttgtagagat cgaaaatcgc catctcccac aactcatcgc ctgcaataca ctcaatgttg
   127441 tcgatacaga ccagcgacaa atgctccata ccgtcgagca cttccggaac aaaccaggtg
   127501 cgtttatcca gcgggacata gcccaccgca tcgccacgct gcgacaattc cgcgcaagcc
   127561 gcgtgcagca gatggctgcg ccccgcgcct tcgcgtgccc agagatagat gtaaccgcta
   127621 tgttcctgac gcagcacgtt ttgcagcgcg gccagtaaag aggagttatc ccccggccag
   127681 aaacttgcaa aggtttcgtc gtcaggaaga taaagtggca aagagagctg tgccggtgtg
   127741 ttcagagata cctcaaccag gatttcacaa aatcgcgaga agtttaccac agaatcccat
   127801 gatgtttgaa ccgggcagca acactgcccg gtcggtacat tatttgtctg ttatatccgc
   127861 gtcttctgcg tccagcacca cttcttcagg gcgcagcaca ctgatcagtt tgaaaatcaa
   127921 acttaagccg ataccgacga tggtcgccag cgccatacct tttaactccg ccgcgccgat
   127981 gtttaccttc gcgccactga cgccgatgat caaaatcacg gaagtcagga tcaggttctg
   128041 tgctttgttg taatccactt tcgattcgat caaaacacga ataccggaag caccaatgac
   128101 accataaagc agcagcgaaa cgccgcccat caccggcaat gggatcatct ggatagcggc
   128161 agccagttta ccgacgcagg aaagcaggat agcgaaaatc gccgccccgc cgataaccca
   128221 ggtactgtaa acacgggtga tcgccatcac gccaatgttt tctccgtaag tggtatttgg
   128281 cgtagagcca aagaagccgg agatcacggt cgacaggccg ttagcaaaca tcgaacggtg
   128341 cagacctgga tcgcgcagca gatctttttt gacgatatta gccgttacta ccaggtgccc
   128401 tacgtgttcg gcaataacca ctaacgccgc tggcagaata gtcagaatgg caaaccactc
   128461 gaagcgcggc gtatagaggg ttggcagtgc aaaccagtga gcattaataa tcggcgtggt
   128521 atcgacaatt cccattgcga aagagagcgc gtaccccacc agcacgccaa ttaaaatcgg
   128581 gataattgcc aggaaaccac gaaacagcac ggaacctaaa accgtgaccg ccagggtggt
   128641 aatagagatg atgatggttt tggagtttgg cgtttgccct tcagccggga gtaaacccgc
   128701 cataccggca gctacgcccg ccagctccag accgatgacg gcaacgattg cgcccattgc
   128761 cgcaggtgga aacagcacgt ccaaccagcc ggtccccgct ttcttcacga taaaagaaac
   128821 caggcagaac agcacgccgc acataataaa gccgcccagc gcgacttcat accctaacgg
   128881 caacagtaac aataccggtg aaataaaggc aaagctggaa ccaagataag ccggaatttt
   128941 ccctttacag atgaagagat acagcagcgt tccaataccg ttaaataaca gtacagtcgc
   129001 cgggttaata tgaaataaga cgggcaccag gacggttgca ccaaacatgg cgaacaaatg
   129061 ttgcaaacta agcgggattg tctgtaaaag tggcggtctt tcactcaccc cgatagcacg
   129121 gcgcgtcata gtattatcct ctgtattatg tgttataggc gctttactca aaaaaaagcc
   129181 gactcttaaa gtcggcttta attattttta ttctttattt cgtaccaaag attttgtcac
   129241 cggcatcgcc gaggcccgga ataatgtatc cgtgctcgtt cagtccctgg tcaatcgatg
   129301 cagtatacag ttcgacgtcc gggtgcgctt tttccagcgc agcgatacct tctggcgcag
   129361 cgaccagcac cagaactttg atgctgctgc agcccgcttt tttcagcagg tcgatggtcg
   129421 cgataacgga accaccggtt gccagcattg ggtcaacgat cagcgccata cgctcatcga
   129481 tgttagaaac cagtttctgg aagtacggta ccggctccag cgtttcttca ttacggtaca
   129541 taccgacaac gctgatgcgc gcgctcggaa cgttttccag cacaccgtcc atcataccaa
   129601 gacccgcacg cagaattggc acaacggtaa ttttcttacc tttgatctgg tcgatttcta
   129661 ccgggccgtt ccagccttcg atagttactt tttccgtttc gaggtcggcg gtcgcttcgt
   129721 aagtcagcag gctacccact tcggaagcga gttcgcgaaa gcgcttggtg ctgatatctt
   129781 gctcacgcat cagtcccagc ttgtgtttga cgagtgggtg tttgacttcc acgatcttca
   129841 tactctttct cctttgaggg gcagccacaa aaaaatcgac ggattatacc tcctttcttc
   129901 agggcggcaa tattattttc gttgacttta gtcaaaatga taacagtttg agataaagtt
   129961 attttatatt caaatggtta tgaaagaaga ttattccatc cgaaaactaa cctttgccct
   130021 ggcacaagtc ttctttcgcc gcgcgcccgg ggaaaagacg tgcaaaaagg ttgtgtaaaa
   130081 cagtctcgca aacgtttgct ttccctgtta gaattgcgcc gaattttatt tttctaccgc
   130141 aagtaacgcg tggggaccca agcagtgacc gataaaacct ctcttagcta caaagatgcc
   130201 ggtgttgata ttgacgcggg taatgctctg gttggaagaa tcaaaggcgt agtgaagaaa
   130261 acgcgtcgtc cggaagtgat gggcggtctg ggcggcttcg gtgcgctgtg tgcattgccg
   130321 caaaaatatc gtgaacccgt gctggtttcc ggcactgacg gcgtaggtac caagctgcgt
   130381 ctggcaatgg acttaaaacg tcacgacacc attggtattg atctggtcgc catgtgcgtt
   130441 aatgacctgg tggtgcaagg tgcggaaccg ctgtttttcc tcgactatta cgcaaccgga
   130501 aaactggatg ttgataccgc ttcagcggtg atcagcggca tcgcggaagg ttgtctgcaa
   130561 tcgggctgtt ctctggtggg tggcgaaacg gcagaaatgc cggggatgta tcacggtgaa
   130621 gattacgatg tcgcgggttt ctgcgtgggc gtggtagaaa aatcagaaat catcgacggc
   130681 tctaaagtca gcgacggcga tgtgctgatt gcactcggtt ccagcggtcc gcactcgaac
   130741 ggctattcgc tggtgcgcaa aattcttgaa gtcagcggtt gtgatccgca aaccaccgaa
   130801 cttgatggta agccattagc cgatcatctg ctggcaccga cccgcattta cgtgaagtca
   130861 gtgctggagt tgattgaaaa ggtcgatgtg catgccattg cgcacctgac cggcggcggc
   130921 ttctgggaaa acattccgcg cgtattgcca gataataccc aggcagtgat tgatgaatct
   130981 tcctggcagt ggccggaagt gttcaactgg ctgcaaacgg caggtaacgt tgagcgccat
   131041 gaaatgtatc gcaccttcaa ctgcggcgtc gggatgatta tcgccctgcc tgctccggaa
   131101 gtggacaaag ccctcgccct gctaaatgcc aacggtgaaa acgcgtggaa aatcggtatc
   131161 atcaaagcct ctgattccga acaacgcgtg gttatcgaat aatgaatatt gtggtgctta
   131221 tttccggcaa cggaagtaat ttacaggcaa ttattgacgc ctgtaaaacc aacaaaatta
   131281 aaggcaccgt acgggcagtt ttcagcaata aggccgacgc gttcggcctt gaacgcgccc
   131341 gccaggcggg tattgcaacg catacgctca tcgccagcgc gtttgacagt cgtgaagcct
   131401 atgaccggga gttgattcat gaaatcgaca tgtacgcacc cgatgtggtc gtgctggctg
   131461 gttttatgcg cattctcagc ccggcgtttg tctcccacta tgccgggcgt ttgctgaaca
   131521 ttcacccttc tctgctgccg aaatatcccg gattacacac ccatcgtcag gcgctggaaa
   131581 atggcgatga agagcacggt acatcggtgc atttcgtcac cgatgaactg gacggtggcc
   131641 cggttatttt acaggcgaaa gtcccggtat ttgctggtga tacggaagat gacatcaccg
   131701 cccgcgtgca aacccaggaa cacgctattt atccactggt gattagctgg tttgccgatg
   131761 gtcgtctgaa aatgcacgaa aacgccgcgt ggctggatgg tcaacgtctg ccgccgcagg
   131821 gctacgctgc cgacgagtaa tgcccccgta gttaaagcgc cagctctgcc gctggcgttt
   131881 ttcaattcac ctgtaaatcg caagctccag cagttttttt cccccttttc tggcatagtt
   131941 ggacatctgc caatattgct cgccataata tccaggcagt gtcccgtgaa taaaacggag
   132001 taaaagtggt aatgggtcag gaaaagctat acatcgaaaa agagctcagt tggttatcgt
   132061 tcaatgaacg cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt gaaaggatgc
   132121 gtttcctggg gatctattcc aataaccttg atgagttcta taaagtccgc ttcgctgaac
   132181 tgaagcgacg catcattatt agcgaagaac aaggctccaa cgctcattcc cgccatttac
   132241 tgggcaaaat tcagtcccgg gtgctgaaag ccgatcagga attcgacggc ctctacaacg
   132301 agctattgct ggagatggcg cgcaaccaga tcttcctgat taatgaacgc cagctctccg
   132361 tcaatcaaca aaactggctg cgtcattatt ttaagcagta tctgcgtcag cacattacgc
   132421 cgattttaat caatcctgac actgacttag tgcagttcct gaaagatgat tacacctatc
   132481 tggcggtgga aattatccgt agcgatagca tccgttacgc gctgctggag atcccatcag
   132541 ataaagtgcc gcgctttgtg aatttaccgc cagaagcgcc gcgtcgacgc aagccgatga
   132601 ttcttctgga taacattctg cgttactgcc ttgatgatat tttcaaaggc ttctttgatt
   132661 atgacgcgct gaatgcctat tcaatgaaga tgacccgcga tgccgaatac gatttagtgc
   132721 atgagatgga agccagcctg atggagttga tgtcttccag tctcaagcag cgtttaactg
   132781 ctgagccggt gcgttttgtt tatcagcgcg atatgcccaa tgcgctggtt gaagtgttac
   132841 gcgaaaaact gactatttcc cgctacgact ccatcgtccc cggcggtcgt tatcataatt
   132901 ttaaagactt tattaatttc cccaatgtcg gcaaagccaa tctggtgaac aaaccactgc
   132961 cgcgtttacg ccatatttgg tttgataaag cccagttccg caatggtttt gatgccattc
   133021 gcgaacgcga tgtgttgctc tattatcctt atcacacctt tgagcatgtg ctggaactgc
   133081 tgcgtcaggc ttcgttcgac ccgagcgtac tggcgattaa aattaacatt taccgcgtgg
   133141 cgaaagattc acgcatcatc gactcgatga tccacgccgc acataacggt aagaaagtca
   133201 ctgtggtggt tgagttacag gcgcgtttcg acgaagaagc caacattcac tgggcgaagc
   133261 gcctgaccga agcaggcgtg cacgttatct tctctgcgcc ggggctgaaa attcacgcca
   133321 aactgttcct gatttcacgt aaagaaaacg gtgaagtggt ccgttacgca cacatcggga
   133381 ccgggaactt taacgaaaaa accgcgcgtc tttatactga ctattcgttg ctgaccgcag
   133441 atgcgcgtat caccaacgaa gtacggcggg tatttaactt tattgaaaac ccataccgcc
   133501 cggtgacatt tgattattta atggtgtcac cgcaaaactc tcgccgtctg ttatatgaaa
   133561 tggtagaccg cgaaatcgcc aacgcgcagc aagggctgcc cagtggtatc accctgaagc
   133621 taaataacct tgtcgataaa ggcctggttg atcgtctgta tgcggcctcc agctccggcg
   133681 taccggttaa tctgctggtt cgcggaatgt gttcgctgat ccccaatctg gaaggcatta
   133741 gcgacaacat tcgtgccatc agtattgttg accgttacct tgaacatgac cgggtttata
   133801 tttttgaaaa tggcggcgat aaaaaggtct acctttcttc cgccgactgg atgacgcgca
   133861 atattgatta tcgtattgaa gtggcgacac cgctgctcga tccgcgcctg aagcagcggg
   133921 tgctggacat catcgacata ttgttcagcg atacggtcaa agcacgttat atcgataaag
   133981 aactcagtaa tcgctacgtt ccccgcggca atcgccgcaa agtacgggcg cagttggcga
   134041 tttacgacta catcaaatca ctcgaacaac ctgaataacc ctatgccaat acacgataaa
   134101 tcccctcgtc cgcaggagtt tgctgcggtc gatcttggtt caaacagttt tcacatggtc
   134161 atagcccgtg tggtagatgg tgccatgcag attattggcc gcctgaaaca gcgggtgcat
   134221 ctggcggacg gcctggggcc agataatatg ttgagtgaag aggcaatgac gcgcggtttg
   134281 aactgtctgt cgctgtttgc cgaacggcta caagggtttt ctcctgccag cgtctgtata
   134341 gtcggcaccc atacgctgcg tcaggcgctg aacgccactg actttctgaa acgcgcggaa
   134401 aaggtcattc cctacccgat tgaaattatt tccggtaatg aagaagcccg tctgattttt
   134461 atgggcgtgg aacataccca accggaaaaa ggtcgcaaac tggttattga tattggcggc
   134521 ggatctacgg aactggtgat tggtgaaaat ttcgaaccta ttctcgttga aagccgccgg
   134581 atgggttgtg tcagctttgc ccagctttat tttcctggcg gggtcatcaa taaagagaat
   134641 tttcagcgcg ctcgcatggc ggcagcacaa aaactggaaa ctttaacctg gcaattccgt
   134701 attcagggct ggaacgttgc aatgggcgct tccggtacca taaaagccgc ccatgaagtg
   134761 ttaatggaaa tgggcgagaa agacgggata attaccccgg aacgtctgga aaaactggta
   134821 aaagaaattt tacgtcaccg taatttcgca tcgctgagtt taccgggtct ttccgaagag
   134881 cggaaaacag tcttcgttcc tggactggcg attttatgtg gtgtgtttga tgctttagcc
   134941 atccgtgaac tgcgcctttc tgacggggcg ttacgcgaag gcgtactgta tgaaatggaa
   135001 ggacgtttcc gtcatcagga tgtgcgtagt cgcaccgcca gcagcctcgc caaccagtat
   135061 cacatagaca gcgaacaggc ccgacgagtg ctggatacca ctatgcaaat gtacgaacag
   135121 tggcgggaac agcaaccgaa gctggcgcat ccgcaactgg aggcgctact gcgatgggcc
   135181 gccatgctgc atgaggtcgg gttgaatatc aaccacagcg gtttgcatcg ccactccgct
   135241 tatattctgc aaaacagtga cttgccgggt tttaatcagg aacagcagct gatgatggcg
   135301 acactggtgc gctatcaccg taaagcgatt aagctcgacg atctgccgcg ctttaccttg
   135361 tttaagaaga aacagttcct gccactgata cagctattgc gccttggcgt attactaaac
   135421 aatcaacgtc aggcaaccac cacaccgcca acattgacac tgattactga tgacagtcac
   135481 tggacactgc gtttcccgca tgactggttt agtcagaatg cgctggtact gcttgatctg
   135541 gaaaaggagc aagaatactg ggaaggcgtg gctggctggc ggttgaaaat tgaagaagaa
   135601 agtaccccag aaatcgccgc ttaatgcttt gcgggccgac gagaaatgtc ggcccgcatt
   135661 attcaggcac tttcgcgaat gggttcgatt tcattcagcg tatcaattaa cggttgcggc
   135721 ttaccaataa gataaccctg catataatcg atccccaaag agagcaccgc ctcgcggatc
   135781 tcttcgtttt caacgtactc tgccactacc cgcattttct tcattcgcgc cagatggcaa
   135841 atcgacgcca caatctgata atccagacta tttgacacaa tattgcggat aaagctgccg
   135901 tcaattttaa gcagatcggc attcacattt ttaagccgtg catagctggc gtagccggtg
   135961 ccgaaatcat caatcgcaat ctggcagcct aattcctgaa gatgctgcaa ggtgatttgc
   136021 gcctgcttaa cattggtcag agcattactt tcggtgactt caaaaataag ttgccacgct
   136081 tcaatctgat atttagccag caactgactg acttcaacag gaaaacgagc ctgacatacc
   136141 gaggttggag acagattaat agcaaaacgg tgagcgggca tcttcgctct gttttcagcc
   136201 ataaattgca gcgtatgctc aatgacccac atgtcgatac tcgacgataa accaaattcg
   136261 tgcgcgaccg gcaagaagct accggggccg atcagttcat cattctcacc tttcatgcgc
   136321 agaagaattt catggtaaac atcaccacgc ataccggtaa tcggctgggc catcaggaaa
   136381 aaatggttgt gttccagcgc ctgctgtagt cgattcatca tcgcgacttt atctttcaat
   136441 tcgcgttgca aatacattgc cccgcaacgc tgcatatttt ccggggcgtt ggtcacgatg
   136501 gaaagttcgg cgaccgtatt tagctctccc agcagcaggt agatatgatt cactggcgag
   136561 cgcacatagc agtaactgac gccaatctgc ggttgcatcg gcatcccatc ccaaaagaaa
   136621 cgaaattgct tgagatgact atccagtgcg gtaatgcgct cctggtgcga ttctgtattc
   136681 agtcgcagcg cgagatcgtt acccgaaagc tgataaacat cttcacccgg ttccagcaag
   136741 ggtgacagcc agtgagaaag tttttgctt
//
LOCUS       DAJTSF010000002        95091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000002 DAJTSF010000000
VERSION     DAJTSF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.002"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(88..1338)
                     /gene="icd"
                     /locus_tag="OO335_000132"
     CDS             complement(88..1338)
                     /gene="icd"
                     /locus_tag="OO335_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6000673.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1510..2163
                     /gene="rluE"
                     /locus_tag="OO335_000133"
     CDS             1510..2163
                     /gene="rluE"
                     /locus_tag="OO335_000133"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6000674.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2173..2634
                     /gene="nudJ"
                     /locus_tag="OO335_000134"
     CDS             2173..2634
                     /gene="nudJ"
                     /locus_tag="OO335_000134"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6000675.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2688..3794
                     /gene="mnmA"
                     /locus_tag="OO335_000135"
     CDS             2688..3794
                     /gene="mnmA"
                     /locus_tag="OO335_000135"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6000676.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3830..4471
                     /gene="hflD"
                     /locus_tag="OO335_000136"
     CDS             3830..4471
                     /gene="hflD"
                     /locus_tag="OO335_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6000677.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4475..5845
                     /gene="purB"
                     /locus_tag="OO335_000137"
     CDS             4475..5845
                     /gene="purB"
                     /locus_tag="OO335_000137"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6000678.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6014..6685
                     /gene="phoP"
                     /locus_tag="OO335_000138"
     CDS             6014..6685
                     /gene="phoP"
                     /locus_tag="OO335_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6000679.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6685..8145
                     /gene="phoQ"
                     /locus_tag="OO335_000139"
     CDS             6685..8145
                     /gene="phoQ"
                     /locus_tag="OO335_000139"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6000680.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8942..9223)
                     /locus_tag="OO335_000140"
     CDS             complement(8942..9223)
                     /locus_tag="OO335_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000681.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9237..10898)
                     /locus_tag="OO335_000141"
     CDS             complement(9237..10898)
                     /locus_tag="OO335_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6000682.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10882..11208)
                     /locus_tag="OO335_000142"
     CDS             complement(10882..11208)
                     /locus_tag="OO335_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6000683.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11264..11446)
                     /locus_tag="OO335_000143"
     CDS             complement(11264..11446)
                     /locus_tag="OO335_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000684.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11430..11870)
                     /locus_tag="OO335_000144"
     CDS             complement(11430..11870)
                     /locus_tag="OO335_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6000685.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11870..12166)
                     /locus_tag="OO335_000145"
     CDS             complement(11870..12166)
                     /locus_tag="OO335_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR6000686.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12163..12501)
                     /locus_tag="OO335_000146"
     CDS             complement(12163..12501)
                     /locus_tag="OO335_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6000687.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12498..13709)
                     /locus_tag="OO335_000147"
     CDS             complement(12498..13709)
                     /locus_tag="OO335_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6000688.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13711..14283)
                     /locus_tag="OO335_000148"
     CDS             complement(13711..14283)
                     /locus_tag="OO335_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6000689.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14323..15480)
                     /locus_tag="OO335_000149"
                     /pseudo
     CDS             complement(14323..15480)
                     /locus_tag="OO335_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15784..15945)
                     /locus_tag="OO335_000150"
     CDS             complement(15784..15945)
                     /locus_tag="OO335_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6000690.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(15955..16260)
                     /locus_tag="OO335_000151"
     CDS             complement(15955..16260)
                     /locus_tag="OO335_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000691.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16257..16535)
                     /locus_tag="OO335_000152"
     CDS             complement(16257..16535)
                     /locus_tag="OO335_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000692.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(16824..18572)
                     /locus_tag="OO335_000153"
     CDS             complement(16824..18572)
                     /locus_tag="OO335_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6000693.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18569..18868)
                     /locus_tag="OO335_000154"
     CDS             complement(18569..18868)
                     /locus_tag="OO335_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6000694.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(18874..19107)
                     /locus_tag="OO335_000155"
     CDS             complement(18874..19107)
                     /locus_tag="OO335_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000695.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19109..19329)
                     /locus_tag="OO335_000156"
                     /pseudo
     CDS             complement(19109..19329)
                     /locus_tag="OO335_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19322..19543)
                     /locus_tag="OO335_000157"
     CDS             complement(19322..19543)
                     /locus_tag="OO335_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000696.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19533..19733)
                     /locus_tag="OO335_000158"
     CDS             complement(19533..19733)
                     /locus_tag="OO335_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6000697.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            19863..20042
                     /locus_tag="OO335_000159"
     CDS             19863..20042
                     /locus_tag="OO335_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000698.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20167..20496
                     /locus_tag="OO335_000160"
     CDS             20167..20496
                     /locus_tag="OO335_000160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000699.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20546..20734)
                     /locus_tag="OO335_000161"
     CDS             complement(20546..20734)
                     /locus_tag="OO335_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6000700.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21109..22338)
                     /locus_tag="OO335_000162"
     CDS             complement(21109..22338)
                     /locus_tag="OO335_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6000701.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22587..23708
                     /gene="roxA"
                     /locus_tag="OO335_000163"
                     /gene_synonym="ycfD"
     CDS             22587..23708
                     /gene="roxA"
                     /locus_tag="OO335_000163"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6000702.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(23757..24983)
                     /gene="pepT"
                     /locus_tag="OO335_000164"
     CDS             complement(23757..24983)
                     /gene="pepT"
                     /locus_tag="OO335_000164"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6000703.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25233..26369
                     /gene="potA"
                     /locus_tag="OO335_000165"
     CDS             25233..26369
                     /gene="potA"
                     /locus_tag="OO335_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR6000704.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26353..27210
                     /gene="potB"
                     /locus_tag="OO335_000166"
     CDS             26353..27210
                     /gene="potB"
                     /locus_tag="OO335_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6000705.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27207..28001
                     /gene="potC"
                     /locus_tag="OO335_000167"
     CDS             27207..28001
                     /gene="potC"
                     /locus_tag="OO335_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6000706.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            27998..29044
                     /gene="potD"
                     /locus_tag="OO335_000168"
     CDS             27998..29044
                     /gene="potD"
                     /locus_tag="OO335_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6000707.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29200..30021)
                     /gene="cobB"
                     /locus_tag="OO335_000169"
     CDS             complement(29200..30021)
                     /gene="cobB"
                     /locus_tag="OO335_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6000708.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30037..30948)
                     /gene="nagK"
                     /locus_tag="OO335_000170"
     CDS             complement(30037..30948)
                     /gene="nagK"
                     /locus_tag="OO335_000170"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6000709.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(30977..32221)
                     /gene="lolE"
                     /locus_tag="OO335_000171"
     CDS             complement(30977..32221)
                     /gene="lolE"
                     /locus_tag="OO335_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6000710.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32221..32922)
                     /gene="lolD"
                     /locus_tag="OO335_000172"
     CDS             complement(32221..32922)
                     /gene="lolD"
                     /locus_tag="OO335_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6000711.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(32915..34114)
                     /gene="lolC"
                     /locus_tag="OO335_000173"
     CDS             complement(32915..34114)
                     /gene="lolC"
                     /locus_tag="OO335_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6000712.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34376..35449
                     /locus_tag="OO335_000174"
     CDS             34376..35449
                     /locus_tag="OO335_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6000713.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35577..39023
                     /gene="mfd"
                     /locus_tag="OO335_000175"
     CDS             35577..39023
                     /gene="mfd"
                     /locus_tag="OO335_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6000714.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39166..40128
                     /gene="ldtC"
                     /locus_tag="OO335_000176"
     CDS             39166..40128
                     /gene="ldtC"
                     /locus_tag="OO335_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR6000715.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40211..40423)
                     /locus_tag="OO335_000177"
     CDS             complement(40211..40423)
                     /locus_tag="OO335_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6000716.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            40708..41340
                     /gene="comR"
                     /locus_tag="OO335_000178"
     CDS             40708..41340
                     /gene="comR"
                     /locus_tag="OO335_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6000717.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41402..41941)
                     /locus_tag="OO335_000179"
     CDS             complement(41402..41941)
                     /locus_tag="OO335_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6000718.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42168..43472)
                     /gene="ndh"
                     /locus_tag="OO335_000180"
     CDS             complement(42168..43472)
                     /gene="ndh"
                     /locus_tag="OO335_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6000719.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(43880..44422)
                     /locus_tag="OO335_000181"
     CDS             complement(43880..44422)
                     /locus_tag="OO335_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6000720.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44445..45470)
                     /gene="nagZ"
                     /locus_tag="OO335_000182"
     CDS             complement(44445..45470)
                     /gene="nagZ"
                     /locus_tag="OO335_000182"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6000721.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45481..46305)
                     /gene="thiK"
                     /locus_tag="OO335_000183"
     CDS             complement(45481..46305)
                     /gene="thiK"
                     /locus_tag="OO335_000183"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6000722.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46286..46927)
                     /gene="lpoB"
                     /locus_tag="OO335_000184"
     CDS             complement(46286..46927)
                     /gene="lpoB"
                     /locus_tag="OO335_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6000723.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(46942..47319)
                     /locus_tag="OO335_000185"
     CDS             complement(46942..47319)
                     /locus_tag="OO335_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6000724.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47322..47681)
                     /gene="hinT"
                     /locus_tag="OO335_000186"
     CDS             complement(47322..47681)
                     /gene="hinT"
                     /locus_tag="OO335_000186"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6000725.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48014..50199
                     /gene="fhuE"
                     /locus_tag="OO335_000187"
                     /pseudo
     CDS             48014..50199
                     /gene="fhuE"
                     /locus_tag="OO335_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50259..51692)
                     /gene="ptsG"
                     /locus_tag="OO335_000188"
     CDS             complement(50259..51692)
                     /gene="ptsG"
                     /locus_tag="OO335_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6000726.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(51987..52784)
                     /locus_tag="OO335_000189"
     CDS             complement(51987..52784)
                     /locus_tag="OO335_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6000727.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(52795..53799)
                     /gene="holB"
                     /locus_tag="OO335_000190"
     CDS             complement(52795..53799)
                     /gene="holB"
                     /locus_tag="OO335_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6000728.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(53796..54437)
                     /locus_tag="OO335_000191"
     CDS             complement(53796..54437)
                     /locus_tag="OO335_000191"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6000729.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54427..55449)
                     /gene="yceG"
                     /locus_tag="OO335_000192"
     CDS             complement(54427..55449)
                     /gene="yceG"
                     /locus_tag="OO335_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6000730.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55452..56261)
                     /gene="pabC"
                     /locus_tag="OO335_000193"
     CDS             complement(55452..56261)
                     /gene="pabC"
                     /locus_tag="OO335_000193"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6000731.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56381..57622)
                     /gene="fabF"
                     /locus_tag="OO335_000194"
     CDS             complement(56381..57622)
                     /gene="fabF"
                     /locus_tag="OO335_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6000732.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(57709..57945)
                     /gene="acpP"
                     /locus_tag="OO335_000195"
     CDS             complement(57709..57945)
                     /gene="acpP"
                     /locus_tag="OO335_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6000733.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58156..58890)
                     /gene="fabG"
                     /locus_tag="OO335_000196"
     CDS             complement(58156..58890)
                     /gene="fabG"
                     /locus_tag="OO335_000196"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6000734.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(58903..59832)
                     /gene="fabD"
                     /locus_tag="OO335_000197"
     CDS             complement(58903..59832)
                     /gene="fabD"
                     /locus_tag="OO335_000197"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6000735.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(59848..60801)
                     /gene="fabH"
                     /locus_tag="OO335_000198"
     CDS             complement(59848..60801)
                     /gene="fabH"
                     /locus_tag="OO335_000198"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6000736.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(60869..61939)
                     /gene="plsX"
                     /locus_tag="OO335_000199"
     CDS             complement(60869..61939)
                     /gene="plsX"
                     /locus_tag="OO335_000199"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6000737.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62020..62193)
                     /gene="rpmF"
                     /locus_tag="OO335_000200"
     CDS             complement(62020..62193)
                     /gene="rpmF"
                     /locus_tag="OO335_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6000738.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62245..62766)
                     /gene="yceD"
                     /locus_tag="OO335_000201"
     CDS             complement(62245..62766)
                     /gene="yceD"
                     /locus_tag="OO335_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6000739.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            62965..63549
                     /gene="yceF"
                     /locus_tag="OO335_000202"
     CDS             62965..63549
                     /gene="yceF"
                     /locus_tag="OO335_000202"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6000740.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(63661..64620)
                     /gene="rluC"
                     /locus_tag="OO335_000203"
     CDS             complement(63661..64620)
                     /gene="rluC"
                     /locus_tag="OO335_000203"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6000741.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65193..68384
                     /gene="rne"
                     /locus_tag="OO335_000204"
     CDS             65193..68384
                     /gene="rne"
                     /locus_tag="OO335_000204"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6000742.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68581..69523)
                     /gene="flgL"
                     /locus_tag="OO335_000205"
                     /pseudo
     CDS             complement(68581..69523)
                     /gene="flgL"
                     /locus_tag="OO335_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69535..71177)
                     /gene="flgK"
                     /locus_tag="OO335_000206"
                     /pseudo
     CDS             complement(69535..71177)
                     /gene="flgK"
                     /locus_tag="OO335_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71243..72184)
                     /gene="flgJ"
                     /locus_tag="OO335_000207"
     CDS             complement(71243..72184)
                     /gene="flgJ"
                     /locus_tag="OO335_000207"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR6000743.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72184..73281)
                     /gene="flgI"
                     /locus_tag="OO335_000208"
     CDS             complement(72184..73281)
                     /gene="flgI"
                     /locus_tag="OO335_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6000744.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73293..73991)
                     /gene="flgH"
                     /locus_tag="OO335_000209"
     CDS             complement(73293..73991)
                     /gene="flgH"
                     /locus_tag="OO335_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6000745.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74044..74826)
                     /gene="flgG"
                     /locus_tag="OO335_000210"
     CDS             complement(74044..74826)
                     /gene="flgG"
                     /locus_tag="OO335_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR6000746.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(74964..75715)
                     /gene="flgF"
                     /locus_tag="OO335_000211"
                     /pseudo
     CDS             complement(74964..75715)
                     /gene="flgF"
                     /locus_tag="OO335_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(75735..76874)
                     /locus_tag="OO335_000212"
     CDS             complement(75735..76874)
                     /locus_tag="OO335_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6000747.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(76899..77594)
                     /gene="flgD"
                     /locus_tag="OO335_000213"
     CDS             complement(76899..77594)
                     /gene="flgD"
                     /locus_tag="OO335_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR6000748.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(77606..78009)
                     /gene="flgC"
                     /locus_tag="OO335_000214"
                     /pseudo
     CDS             complement(77606..78009)
                     /gene="flgC"
                     /locus_tag="OO335_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78013..78429)
                     /gene="flgB"
                     /locus_tag="OO335_000215"
     CDS             complement(78013..78429)
                     /gene="flgB"
                     /locus_tag="OO335_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR6000749.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78584..79243
                     /gene="flgA"
                     /locus_tag="OO335_000216"
     CDS             78584..79243
                     /gene="flgA"
                     /locus_tag="OO335_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6000750.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79319..79612
                     /gene="flgM"
                     /locus_tag="OO335_000217"
     CDS             79319..79612
                     /gene="flgM"
                     /locus_tag="OO335_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR6000751.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79617..80033
                     /gene="flgN"
                     /locus_tag="OO335_000218"
     CDS             79617..80033
                     /gene="flgN"
                     /locus_tag="OO335_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6000752.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80073..81608)
                     /gene="murJ"
                     /locus_tag="OO335_000219"
     CDS             complement(80073..81608)
                     /gene="murJ"
                     /locus_tag="OO335_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6000753.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(81808..82641)
                     /locus_tag="OO335_000220"
     CDS             complement(81808..82641)
                     /locus_tag="OO335_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6000754.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(82643..83290)
                     /locus_tag="OO335_000221"
     CDS             complement(82643..83290)
                     /locus_tag="OO335_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6000755.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83301..83885)
                     /gene="rimJ"
                     /locus_tag="OO335_000222"
     CDS             complement(83301..83885)
                     /gene="rimJ"
                     /locus_tag="OO335_000222"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6000756.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84121..85329
                     /gene="mdtH"
                     /locus_tag="OO335_000223"
     CDS             84121..85329
                     /gene="mdtH"
                     /locus_tag="OO335_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6000757.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85393..86040
                     /gene="grxB"
                     /locus_tag="OO335_000224"
     CDS             85393..86040
                     /gene="grxB"
                     /locus_tag="OO335_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6000758.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86174..86734
                     /locus_tag="OO335_000225"
     CDS             86174..86734
                     /locus_tag="OO335_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6000759.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            86840..87886
                     /gene="pyrC"
                     /locus_tag="OO335_000226"
     CDS             86840..87886
                     /gene="pyrC"
                     /locus_tag="OO335_000226"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6000760.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            87960..88205
                     /gene="dinI"
                     /locus_tag="OO335_000227"
     CDS             87960..88205
                     /gene="dinI"
                     /locus_tag="OO335_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6000761.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88495..88749
                     /gene="bssS"
                     /locus_tag="OO335_000228"
     CDS             88495..88749
                     /gene="bssS"
                     /locus_tag="OO335_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6000762.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            88864..89982
                     /gene="solA"
                     /locus_tag="OO335_000229"
     CDS             88864..89982
                     /gene="solA"
                     /locus_tag="OO335_000229"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6000763.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90030..90143
                     /locus_tag="OO335_000230"
     CDS             90030..90143
                     /locus_tag="OO335_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR6000764.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90404..90970
                     /locus_tag="OO335_000231"
     CDS             90404..90970
                     /locus_tag="OO335_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6000765.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            90974..91549
                     /locus_tag="OO335_000232"
     CDS             90974..91549
                     /locus_tag="OO335_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6000766.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(91591..92643)
                     /locus_tag="OO335_000233"
     CDS             complement(91591..92643)
                     /locus_tag="OO335_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6000767.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            92868..93788
                     /gene="lpxL"
                     /locus_tag="OO335_000234"
     CDS             92868..93788
                     /gene="lpxL"
                     /locus_tag="OO335_000234"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6000768.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            93960..94157
                     /locus_tag="OO335_000235"
     CDS             93960..94157
                     /locus_tag="OO335_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR6000769.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94181..>95091
                     /locus_tag="OO335_000236"
     CDS             94181..>95091
                     /locus_tag="OO335_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6000770.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGVGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGII"
BASE COUNT        22882 a        25209 c        23475 g        23525 t
ORIGIN      
        1 tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt gttatacgcc
       61 cgtttaaata tttaacacat gtagtgatta catattcttg atgatcgcat caccaaactc
      121 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      181 ggcgttgatc gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      241 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      301 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      361 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      421 accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      481 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtat tcgggttttt
      541 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aattcttcac gcgccagctg
      601 gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
      661 ggtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
      721 ggtgccttct tcagaacacg gcttaatacc gataccgcaa tgttccggga agcgaatttt
      781 cttcacgccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtcagc
      841 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
      901 cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg gacgcaggca
      961 gatgtagaga tccagttcct gacgcagggc aacgttgaga gaacgaatac cgccaccaac
     1021 cggagtggtc agcgggcctt taatggcaac gcgatattca cgaatcagat caagggtttc
     1081 agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1141 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1201 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1261 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1321 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1381 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1441 atttgtaatt gacttgtcaa tactacccta ttgtttgccg tcatgaaaga tgcgcgttat
     1501 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1561 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaaca tgaaaaccag
     1621 cccacgcgtg tgatcctctt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     1681 accggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgc
     1741 cttgaccgcg atagcgaagg gttgctggtg ctgaccaata acggcgcgtt gcaggcgcgt
     1801 ttaacccagc cgggtaaacg caccggaaaa atctattatg tgcaggtgga aggtattccc
     1861 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     1921 cccgcaggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     1981 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     2041 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2101 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2161 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2221 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2281 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2341 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2401 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc gacccagcct
     2461 catgacagcg atatcgactg ctgccgttgg gtcagcgccg aagaaatttt acaggcgtca
     2521 aatcttcgtt cgccgctggt ggcggaaagt attcgttgtt atcaaagcgg gcaacgttat
     2581 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     2641 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     2701 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     2761 tgcaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     2821 gtgaggaata ttgcacagca gcagcggatc tggctgatgc ccaggcggtc tgcgacaagc
     2881 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     2941 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaagg
     3001 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3061 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3121 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3181 aaagcctgtt cccggtaggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3241 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3301 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3361 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3421 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3481 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3541 tcgggttgat tgcccagcag ctgcactggg tcgatcgcga accattcacc ggcactatgc
     3601 gttgcacggt aaaaacccgc tatcgccaga ccgacattcc gtgcaccgtc aaggcgctgg
     3661 acgacgatcg cattaaagtg attttcgatg agccggttgc cgccgtgacg ccgggccagt
     3721 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     3781 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     3841 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     3901 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     3961 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     4021 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt tgccagggct taaacgccga
     4081 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4141 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4201 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4261 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4321 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4381 cggcggcgga cgtctacaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4441 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4501 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4561 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4621 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     4681 aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     4741 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     4801 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     4861 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcgtca
     4921 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     4981 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     5041 ccgtatggag cgccagcacc gccagcttaa ccaggtggag atcctcggca aaatcaacgg
     5101 cgcggtcggt aactataacg cccacatcgc cgcttacccg gaagttgact ggcatcagtt
     5161 cagcgaagag ttcgtcacct cgctgggtat tcaatggaac ccgtacacta cccagattga
     5221 accgcacgac tacattgccg aactgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5281 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5341 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg acttcgaaaa
     5401 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5461 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5521 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5581 gaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg cagaaccaat
     5641 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagcttac
     5701 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     5761 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     5821 cacgatggtt gatgagctga aataagcctc aaatcagtgc cggatggcga tgctgtccgg
     5881 cctgcttatt aagattatcc actttttctt ttttcacttt acctcccctc cccgctggtt
     5941 tatttaatgt ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca
     6001 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     6061 taaagttcag attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc
     6121 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga
     6181 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaac gatgtttcac tgccgattct
     6241 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga
     6301 tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6361 gcggcgtaat agcggtctgg cttcacaggt catttcgctc cccccgtttc aggttgatct
     6421 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6481 cattatggaa acgttgatac gcaataatgg caaagtggtc agcaaagatt cgttaatgct
     6541 acaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6601 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtc attaccaccg ttcgcggcca
     6661 gggctacctg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctcg
     6721 ctgcgggtac gttttctgtt ggcaacggca gcggtagtac tggtgctttc gcttgcctac
     6781 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6841 cgtggcgaga gcaatctgtt ctataccctt gcgcagtggg aaaacaataa gttgcatgtc
     6901 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     6961 gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaaaatgat ccagcctgac
     7021 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     7081 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     7141 gcggagatga cccactcggt ggcggtaaac gtctacccgg caacatcgcg gatgccaaaa
     7201 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7261 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7321 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7381 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7441 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7501 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7561 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7621 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7681 ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac
     7741 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7801 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7861 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     7921 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     7981 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     8041 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     8101 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccttttacc
     8161 actacatcaa gtcattgatt cgatcaaaca attttcattg aaaaagtgcc aaaaatcgca
     8221 aattgactac accattaact acaccgttcg gtgcactgta tgaaacaacg tggaacaaat
     8281 agacacaaga aatatacagg cgggtaatct ttccaggggg aagcgcacca attcatgagg
     8341 ggcgttaatg tcgatatggg gatccccata acggggctac cggatttttt ttccggttaa
     8401 cctttaatca ggctggtggg ttttacatac ccttgatcac cgtaatgatg atccagatgt
     8461 gcaccttttc caccaatggc ggaaaagctc caaccgtaac gatgatcgct atggttgcct
     8521 cgatggtctg cgtggtcatt atgaccgttc agccagtgac cttaatcaga atttttcgct
     8581 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatgaac
     8641 ggttaaaccg tctttttgtc acggtgtgtc actggttgtc actctccgtc acgattgagg
     8701 tggtggtcat tctgcccgac cgcgcatctt tgcgctatcg ggaatatcaa acagttacct
     8761 cgccgacttc ctcaaattga ggtttccgaa aatatcagag cgggttagcg attaagctga
     8821 cgagtaatta aactgcgaaa atttcgtagt ttctgaatat attccggcct gctggttaac
     8881 agtcctcaga tgtgagggat gtagccagcg caattttgcg ctttgcttta atctcagtga
     8941 gttaccgccg taaccgctcc ggcttcttcc agtggtacgt gattttctcc ttctcccgat
     9001 acagttcaac gcggcgattg taggccagca tttccagaac gcggatccgt atgtcgcgca
     9061 tatccgcatc attaagctgg ataccatcac ggcgcatcac ctcagcaaca acacgcgcat
     9121 aattttcggc tgtcacgctg tccggctgcg tggtctgttc gtcagcctgc tggctgattc
     9181 cagagacgcg gcggattaat cgcagtattt cggcttctgt catgctgctg acctcattac
     9241 accccgctaa attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc
     9301 tcatgctctg cctggaatgt accaaacgcc atcagcgccg ctacgctcgg gtctatcttg
     9361 ttcggtgatt ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg
     9421 ttactcatcg accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca
     9481 aaaacttcga acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc
     9541 acctcaaaac cagccccctg taattgcgtc ctgagatgcg tggcgttcca cgtatcaaag
     9601 cccaccagcc tgatattaaa tttctccgca tcctgcatga tgtcatctct gatccggtcg
     9661 taatcaatgc agtcacctgg cgttgtgcgt atccagcccg ctttagccca ctggcgatag
     9721 acagcgcggt ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa
     9781 atcagcataa tggttttacc gaccggaaag gcatagcata cgctggaaat atcgctggtt
     9841 gatgataagt ccagccccgc gtaacactcc tgaccgtgta aatcttcctc cgtgaacgtt
     9901 ccggcacact cagcccatgc accattaccc atccacgggg tagtcccctg acaccagata
     9961 ttaaatcgct ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg
    10021 gttgaggcca gtttttcacg atccacggaa acatcaatat tagggttcgc ctttatccac
    10081 atcgccggat catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa
    10141 tcgttaacct cttcgccgtc caggatctgg cagcaataat catagtgctg tttacaggct
    10201 gaaacgacgt tactgcccga tgtggtaatg gcaaataaca gcccttcggg acgtgcgccc
    10261 atccccagct caagcgcgga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg
    10321 atggaaagac tggggtttgt cccctcgatg gttgccgctt ttgctgccag cggctttaac
    10381 aggctgttgc ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac
    10441 ggtcgggata aaaggcacat ctgacgcgca ttatcaaaca cgatccgcgc ctggtcacgg
    10501 ctcaccgctg cggtgtagat atcctgctga ccgttctcca taatcagaaa ccagttagcc
    10561 agaatcgcgg cggtcgtgga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg
    10621 tatttgcgcc gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc
    10681 tgccacggct ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc
    10741 ctggaaaagg caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta
    10801 aggtccgaaa agtaccgttt aacagcctgt tttaaccgtt tacaggccgg aattttgccc
    10861 gtttttacgt cttctgcgta cttattccag gcggtcaagc tcgtcctctt cttctgtttc
    10921 cggtggattt ttacggcggc ttatcgggtc aaaaccgagc aaggaggcca tttttatcat
    10981 cactctttca gcgtcggatt ttgcgcttaa tgcggggttt ctgctctcgc cgccctgact
    11041 gttaacaatg ctgaacccgc gcgccgcaag gtctgcaacg gctttccggt aaatggagta
    11101 gttgacgcaa tacagttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc
    11161 aagctgccgc gatttttccc gccactgctt cacggcgata tcatccaggt aggcggggtc
    11221 gggtttgtcc tgaaaacgcc ccccaccccg tcatacagcc tcatcagcga ttgcggaaac
    11281 attccataac ctcgcggtca cggtcggtta atcgcttcgc tgtgatgcgt tctgcgcgtc
    11341 ctgacgcttt atctttatgc cctgtttcct gtgtcttcca tgcgtcacgc tgccttataa
    11401 gtccacggat aaggcggttt tgttcccgct cattcatcag cgtcatacat ccagttattg
    11461 cggttagcag cccgttcttc ctcttcacga aatccacctg cggcacgctt gctttttgtt
    11521 gccggatcaa gccatttcgt tttctggtta tgacacgcct ggcacaatgg ttgatggttc
    11581 cattcgggcc agaagagaac atcatcaccg ccattaatcg ggataatgtg atccaccacc
    11641 acggcgggcg tatatatccc cttctcaagg catcgcacgc ataacgggtt tttactcaga
    11701 tacatggcgc ggtatttgtc ccactgtctg gaatatccac gcgcgcggcg gtgtcctcgt
    11761 ctggcatcct ctgcacgcca tgccgcccgc ctgtgctcct cacacttgcc ggatttaacg
    11821 cgcctgttac agcctggttc tgtgcatcgt cttaatggtt gccacggcat cagtaccccc
    11881 ccacatcacg gtaagccgtc cagagtgcgc ctatcgtcat gggtacgcgt gtttttgcgt
    11941 tatccgcgac aatctggcga ttttcataca ggtgagcgat aaacatcatg cagccaatct
    12001 ttatggctgg cgtgaacgcc agcccgtcat caaagcgcct gcctatgtgt atctggcacg
    12061 cctcaagcga tgcggcaatg tatccgctga ttaactggtc ttcatcgtcg ccatcgatgc
    12121 ggcaatggag tttcacttct tccagggtga taagttcttc tgtcattttt ccgcgccctc
    12181 acgacaaaga atttcaaggc gtgtcctggc ggcatccggc agcggctgcc cgatgatatt
    12241 cagtacacgc cccgccagcg gccccgtact gacctttatc cggctggtgg cgttgatgtc
    12301 cttccggtac cgtatccaga tccttacggt accaatcgcc agctctgcgc ccgatgaaat
    12361 ggcctccttg ctgctgacca tgttcacgct tgcccagagt gtgtgaccgt cctcccacgt
    12421 ttcgagtatt tcgccggtta tatccctggt ctgtttcagg gtctgaatcg ttatcctgtc
    12481 acgcagtcgc cctatgttca ttccggtttt tctccctcgc tgatttttac ttcctgtttc
    12541 catgcctggc tgaactcatc accaccatca cgcggtgaca gcccttcacg ttcgcgggct
    12601 tcgttcggac acataacgtc ggattttatg ccgcgctcat aggtggcaaa gcgttcgcca
    12661 ggtgtggccc gtaacaggtc cgcgctgtca aactccacct gataccggat accaggtaca
    12721 ggcgaggcca ccagcaaggc gttttttatc tgctgctcaa agttcgccag ccacgggcgc
    12781 atcgtcatgg tgagaaatgc gcggcttgcc tcgctgaagt tgctgtaggt gctgttgctg
    12841 tattcctgca aaaaaatcgg cgagacgttg aacattcggg cgatgtcttc aatggtgaag
    12901 cgacgggagg ccagccattc ggcatcctga ttactcatgc ccagctgctg atagctcatc
    12961 cccccttcaa ggatgggcgt ttttccggcg tttctggccc ctttgtagcg ttccagtgcg
    13021 gctaatgcct gtttgccctt cacgccgtcc agccattcgc ctgacgtgat aacccctgcc
    13081 gccatcatgc catcacgcat cacgctcgcg ccgtggcgtt gttgggccag cccaagcccc
    13141 agcgattcgc ggcagatggt tacaggtgag cgccccataa aaccgtcatc cgtggagtaa
    13201 cgaaggtgga gaacctccca cggtaaatag ttgcggatgt ttccggtgta ggcgtcagtg
    13261 atgcagtagc gccagttatg atcacctgtc tgctccacgt tcaccgactg cggcgggtaa
    13321 ggatgtaaag ccgccggaaa accatcacgc ccccactgaa taaccgcata agcattacca
    13381 cttaacaggc agtagcggat catcattcgc ttaaactggt agggtgtctg ccacgcgttc
    13441 gggcgctcgt tgaggatgtg atcgaccgga tgagaatcaa gccattcgcg ggcctccttc
    13501 cccttctcat tgcgtaccag gtacaggtaa cacggcatgg tagccaccgc gtcagagatg
    13561 accgtgacgg cgttcatgac ggcggggagt gattccgctg tcccagatga tacgtactca
    13621 cccgcccccg tgttcgatgt gccggccagc gccataaatt catcaagcgt catgctgcgc
    13681 tgctcttttt ttcgtctgaa aggccacata tcacaccccc gctaaatccg cccaccagcg
    13741 gcgattatcc gcacgcggca ttttttcggg gtgctgctca tacagggaac ggcgggccag
    13801 ctccacgccg gaatcggggt aagccggtac cgatgtaacg gtaatttcgt acagttccgc
    13861 caccagcacg gtgcgcacgc atggatctgt tgtggtatcc catacatcct tacgggagcg
    13921 aaagccaaag ctcatccccg agatgtcccc acgcttaacc agttcgataa cgtcgcgccc
    13981 tgtactggta tccggtgggg tcagttcaaa gcgtaaccct gtttcgtcct cttccagttt
    14041 cagcgtgccg gaacgggtgc gccccagtaa catgctgtgg tcatgctcat acaggccgcg
    14101 aacgtcatta cccgccgcaa gccattcagt aaacgccccc cgctggaatt tttcgtaaaa
    14161 ctcaccccat aacagttctg aaagcttatc ccagcgaaca acgtagcccg tcagcgtacc
    14221 ggctccgctg gtggtgattt ccgatgaccg gatttccata ctcttcataa ttttttcacc
    14281 cataaaacac taaaggggct ttttagcccc ttctgtatgc tgttaatcgt cgtcttccgg
    14341 cagtgccaga atcttgatcg cgttcgaatc caccacgcca ccgcctaaat atttttgcgt
    14401 gaaaattttg atgaatcctg gttctgtcag gttgtccggt ctggtgcgaa cacctgtttc
    14461 gtgatcaaca atgtagtaac cgcgtttgaa gtcacccagg gcaataacgt tatcaggcat
    14521 aaactccaga tattcgaccg gaaggcccag caacgtatca ggatcacccg cctgtaaacg
    14581 gtcgcgccag atataatcac cgttcgcgtt cttcaccttc tgaagttttg ccgccgtcgt
    14641 ggagttaacc actcagaccg cgtttttgcg gtatttttta cgtaatgcaa atttcaggtc
    14701 gatcagcggg tccgcagatg tccacgccag agattcggaa ggtttaatta cctgcaacgt
    14761 accaaaatca cgctctttgt cgttcttctc tgcacggggt acggataaaa aaccttttgc
    14821 ttttttgtca ccgtcgccca caaccagatc gctttcttcg gtttccgtga aggtgtcgcc
    14881 aatctcaccc gtcagccatg aaaggatgtc cacatcggaa aaatccacga tttcctgtgt
    14941 ggtgcgcggg tacgcataga ccggatacag cttaatgctc acctcgttaa tctgcggggt
    15001 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
    15061 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc gacgcatggt
    15121 ggattcatcc gccagcattc gcatgatttc ggtgttcagt tcagggataa cggtataacc
    15181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca ccggtcagaa tgtagttacg
    15241 tagttctgcg gggtcagtgg tctggctgct tttacctggc ttgctgcgct cttcgtctgc
    15301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagcgc gtaattcgtc
    15361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttcagcgt tctccagcat
    15421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctctgcat
    15481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaacactc aaccgttttt
    15541 cataaggaaa tcagtaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc
    15601 acattaacag gcgggaaagt ggccccagcg tcctggcacc acgggcgaga ataatcatga
    15661 ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa cgtggaacaa ccacgaacaa
    15721 ataatttaca aaaaataaca aaaagccgga ttgctccggc tgcgtggtgt ggttctgata
    15781 ttcctactct gctaatttgc tcagccccat gtctgcgtaa gtgcgattta ctgcatttct
    15841 caggtctgcg tagttctccg ttggcggctc cggtgtgccc ttgctgctct gttttaccag
    15901 cgggtcgccc accagctgcc cgtaagctgc aatttgtgct gtcatattgc cacctcagtg
    15961 aagcgatccg gcattgttct ttttcatctc ttccatcatt ttttcagtga gcattacgca
    16021 ctgaaacccc atgtttttga tgtctacggg tttcatgcct ttgtcagtgg atatcacagg
    16081 cagaatatcc tgatacacat cgcggtcagg gtaactaagc acacaaagct cccatgatgc
    16141 cacgggtgag gatccttcgt ctgtttcaac ccaccagcct ggtgtagctg cgcttatttg
    16201 cagaatcgtg atccgtccgt tgtgcttgta aaatttttca ccaaattttg gttcgctcat
    16261 gattccacct ctccggtttt aacgttgatt gttgttacct gttccgcttc ggcaatctcc
    16321 cgctctgtca gcgtggcaaa atttgccgcc gtcgtggtca tgaatgcgct tatcagttcg
    16381 ggatgtgctt tcgcgtatcc ttcccccgcg tggcggtcta tcgttctgat tgccaccttt
    16441 aaagcgtgct ctgtcatgtc taacgcgcga tatttcggtt ctgttctgtc tctgcgtttt
    16501 ttgaatgatt tattaaattt ccctgaagtg tgcatattta tttttacccc ctcgtttaaa
    16561 aagttttgag ttgtgcctcc ccttgtctac cttatctacc ttagtggccc tcatgccagt
    16621 aatggcgctg ctttcagcgg ggtagagtgc ttttatccac tatctacccc gtgtctacct
    16681 ccctgtctga ttcaggtaaa atcaggtaga gagggtagat agtgggtaga cagtaaaaaa
    16741 aggctatcta cctaacttaa tgcactgaat taaatgtatt tttctttact caggtagaca
    16801 gggtagacag caattacaaa aaattataaa aacgcgtcgc actcgtctgt tgttattgcg
    16861 ttagtctgcg ttactccctt aactttccgc gtaatatatt catgtccgta aactttcgcg
    16921 gctggcttca tagccttgcc aaagtcattt acgtttagcg gtttgctcct gcctgcgtac
    16981 gccataaacg ccagatagac gcggtaaagg ctgtttctgg tcgtgtactt cactgaatcg
    17041 ccaccgccgc ccatcatcag gccgcgtgct tcctccagaa aattcaggaa ctggcaaaac
    17101 tcaataaccg gatccgtctg ttgctttatt gccagagctt catcaccgtc acgctgttcc
    17161 agtagtaaag cccgtgcttt ctcagagtcg gtaaagttcg ccagcaatcg gcggataatg
    17221 acagggattt cagccgcaat cttttccggt agctccctgt ctttttcggc ctcactaacg
    17281 atattgtcga aacggaaaat cacgcgacga cgtgccacac ctccggcccg ttcggtgaat
    17341 atcatcgggt tattgttggt cgccagcacc accgccctga ttacagccgt gaaacgcttt
    17401 tcgtatttcg ggttaatttc cacggggtca ccgcccgtga ttttcttgat gcccgttcct
    17461 tcgcctgtat atttcggctg gtcagccagg acgataagac gactcccgac aacctgcgca
    17521 cgtccaccag catcatcaag cgatgtcatt tcagcgctta ccgtgttctg tttccctgcc
    17581 agaaggctgg ctatgtgtgt gaatgtactt ttaccgctcc cgccgtctcc ggtggcctca
    17641 ataaacatct gccagtcgta ccggttcgcc ataatcatgt acagcgcggc acatatacgc
    17701 atcatcttgc gctggtcttt tccggctgcg tgctcaagcc atttatgaaa gtttagcgcg
    17761 ttatcgcgga tgttctcccc tggtgctggt ggcgtgtact caatgccgtt gtgcgtggtg
    17821 atccagttct ccggcgtgtg cggggaaaat tcccccgttt tcaggtcaag cgcaccattg
    17881 gcgaacggca gcaaatcgcc ggacggctcg cccattggtt cggcaataac ttttaacgct
    17941 tccacggcgt tattgattac gcgcttgctg aaagtggccc tgtgctctga atagatcacc
    18001 accatttcgc ggctaagttc cattgtgctg accggacacc ataccccgcc gcgccatacg
    18061 tgaacgattt cactttcctg atgcacacaa acgccatcaa agcgctcggc aagcagctgc
    18121 gcgcgctcac tgtctgccat ttgtgccagt tgcgtatttt tctgctccgg catggtaagc
    18181 cctgcggcaa tattttcacg ctcagcattc agatagcgcc gccagttttc acacttctgg
    18241 acgtgcattc cttcggggta aaaatttgcg tcctgaatat ctgccgccgc cagcttctga
    18301 ccaatctttt tggtctccac caaatccagt tctccggcct ggtacagcct tacgcgtttt
    18361 tttccatccg gaacaatttt cagtgcatca agttcggcaa gctgatttgg cccaagccac
    18421 acaggcggca cattatcgcc ggatgcgggg ccatcctgct cctgccattg tttcgcgtgc
    18481 gaccaggcat cactacccgc aaagataatt acctctgtgt ctttgtgttt tattccgcgt
    18541 ggctgttttt ttacgttcgg tgccagtttc attttttacc cctgaatacg ttaagcatct
    18601 tttttatttc ctgaatattg gcgcgtgctt tctccctgct ggttggttta ctgcggggcg
    18661 ctgcctgtac cagcgtgaaa tcacgccgga attgataaac aggcatcacg caatcatatt
    18721 cgtaaccttc acggcggtag gtgatgcgcc gttctgccac gcctttaatc gttaccatgc
    18781 cgccgtactg gtcgcggtaa atatcaccgc gcgtaaattt agggtgagtg ttgccactgg
    18841 cagttaagcc agaatattta agtttcatta tttttattct ccggtgtgtt gttctttata
    18901 tctgtcgtgc aatagatcta tttcttgcag ttccattatt acaggctcaa gaagcgttat
    18961 taatgccgtg gcaattcttg atttttgttt gtcgcgttca ttgtcgccaa gtgtttcaag
    19021 ccatatgcgc aatatttcca gcatgttttc actgtgagaa agtgcaagaa atgcgcggtc
    19081 tattgtttcg tggtaaatat cacgcatatt aatccccgtc cgtcgttttt cttaaaataa
    19141 cctctgtcac gaaatcagca taatcggcgg cgatattcag tacatcaagc ccgttgattt
    19201 atattctctc gtggaaagta agaaaaaagc cgctctaata agttctggca ttgacgaaag
    19261 cgcatcagcc gcatcatcag gaacgccgga aaattcctgt ttcagggaat taaaacgatc
    19321 atcacgcata acccccccca ttttcacaat cggcaacaag aatattttta gcgtcattca
    19381 gtgaccgcgt tgcgatgtat cggatacatt ccagggcgaa ctgtgtccgg tctttctgtt
    19441 cttctccctg cgcaagctct gccgtgcatt caatatcaat aagcgcgtgc atcagcgtag
    19501 tgagtgcggc ggctgctgcg ttcggtgtgg ttttattgtg cataggtttc cccctgacga
    19561 atacgggcgg cgaatacagc aacacaaccg gacgggcaac ggctacgcgc ttcgcgttcc
    19621 gtccaagcgg ttacgtggat gatttgggat tctccggcac tcagtgccag aaaacgccac
    19681 acaaaggccg tttgtgtgtg tacaaggtgt ggtatatgat ttacggcaac cataacggct
    19741 cctagtttac gttgttggtt agaagccccg ttactgctcc tacagtgcgg ggctttgctt
    19801 tttgtgttga ttgtgtgtaa tgtgtcacta cacataaata cattacctga ggtgtaatta
    19861 acgtgtcaac acacaaaaac gaaaggcgtg gtaatcctcc gttccagttc cgtttagatc
    19921 ctgacttacg ggaaaagatg gaaaaggcac aacaacagga cggcgatgat tctcttgctg
    19981 cctggataaa gcgaataatc cgcaaagaat tacagcaacg cggcatagaa cccaaaggat
    20041 aaaaactatc agcgccgtgg tgtgaggtac tacggcgcat tgctttacag gcgcacacaa
    20101 tgaccaacga agaatcaacc gaaaaaacat cacctgcacg aaaaagacga cgcagaaaga
    20161 aagagcatga atcagaacga ttcgcgccgt gttcttttgc ccttgagaag taccttaaag
    20221 aatactcatc aaaggagaga acttcgcagt tatggcaacg cactgaacca gactgattgc
    20281 attgcccacc agcctgatag cggctatcat ccccgtgctt atgtttggga tcacacacac
    20341 aaacggcgca gcgggttatc tgttcagaaa ggcggctcca tttcggggcc gcttttttta
    20401 tgcctgaaaa accccaattt tgtggttttc cagtttcacc agggcgaacg aatccccgcc
    20461 cacgttctgg cgtatattca atcttcatgg ttatagctct gtgttcagat gattgatgtg
    20521 tggcggctgc gtgccgccag cgtgattaat gaactgcctt gcagctatcc ttccaggcca
    20581 gaacctcgga taaagaccag ccaacggaac gaccgccaag tttacgacgt gatgggaatt
    20641 gtccggcctt ttccaggcgg tagcggcatg agcggctaag gcctgttagc ttttcgcatt
    20701 ctttttcacg tataaaccga tcagtgctta acactattgc cccctttcgt ttcttaaaga
    20761 gttatttcgt gttctattgc gttgggatgt gtctgattgt gtcaggatga ttcagagttg
    20821 gcaaatgttg aggccgtatg gtttacagaa agaggaataa tcaggataaa atcctttaaa
    20881 ttcatgttaa tacaaaggca taaaatattg tttcatgcct tttttctcgc tatttaaaga
    20941 gtgattcgct agtgtataaa aaatcagtaa cacattaaaa atcagctact tataaatctg
    21001 tacacttttt cacctcttgt tcgtagttgt tccgcattgt tgctcattgt tgcacgttgt
    21061 atctgttcgc atatccagta tgcgcatact gaaaaaacac gaaaaaaatt attttcttct
    21121 ggctactggt agcgtggtta cgttttcatg tgttcccgcc agtatcccta accgctccgt
    21181 ccacatatcc agcgcattgc gtttagcatc cagataacgg gaatgattat aaactcgctg
    21241 cattcctggc atctggtggc ctgtaagctg ctccacgacg tgaggatcca cgcctaaatc
    21301 gttcagcatc gttgtaaagg tgcgccggat gtcatgtagt gaccagtgag ggtgatttag
    21361 cctcctgtgc gctaatctgc cgtactgcga cacgcttgtt tcctgtttca cttcccccag
    21421 caataagccc gtgtgcctgt tctgctccac cagctgcgtg acgaacggca gtattgcttc
    21481 cggtatgggc cggaatattg ccaccttcgt tttgctgtgt tctttcggca cggtccagag
    21541 catttcggta aaatcccact cgctgatctc cgataacctc agttctaccg tccggcatcc
    21601 gaacacaatc aggaggcgga ttaacgcgat gtagtagggg gagaatattt ttttgtccag
    21661 tgcctgcaat aattcgccca gttctttggt gcttaagaca cgctcgctta tatccggttt
    21721 tttcccaacg tccgccacac tcatatcatc aagaacgttg ctgattgcat agcgccgcct
    21781 ccggcagaac ttaagcgcct gtttgcacgt ctgtagcaag aatccggcag taacaggcgt
    21841 tcgctttgcc acctggtcaa aacaggccag ccagtgccgt agctcgcatt tatccagcgg
    21901 catagcacca atgtgctgta ttacgtgatt attaaggcgc tttttcaggg cggcataatc
    21961 cacgcggttt tcctttgcgt acgactcaag ccagtaggtg agcgcatcgc caaccgttac
    22021 cggctttaac gcttcctgta cggtgtaatt aagctcatga cgtggatttt tcccctctgc
    22081 cagccatgcg cgacactggg cggctttttc cctggctgat ttcaggctca gatcaggata
    22141 atttcccagc ttaatgcgtt ccggtggtgc cccccttccc gttccggccc tgtaagtgaa
    22201 ataccaggtt aaaaggccac tggttgaatg cctgacgctc aggtttccac cgtcattaag
    22261 aaaggctgtt ttttgggcgg gtgtgccgtt gattttcctc agctgtgtat cgctcagttt
    22321 gttaagtgct ctgctcataa ttttgattcc ggtcatcaca attaactaca ccattaacta
    22381 caccgatcgt tgcacaaagg gctacaacgt gaaacaagct ggaacaagga aaatcacaaa
    22441 tctgtttata atcaaaaaca taatgaacaa tctgaaacat tatgaaacgg caaaaaacac
    22501 taattgataa aatatgtcca tacttcacgc attacgttaa gcatccgtta taatcggttg
    22561 cagataccag cctgtggatg cttaacatgg aataccaact cactcttaac tggcccgatt
    22621 ttcttgaacg tcactggcag aaacgcccgg tggtgttaaa acgcggcttt aataatttta
    22681 ttgacccgat ctctccagac gagttggcgg gtctggcgat ggaaagcgaa gttgacagtc
    22741 gactggtcag tcaccaggat ggcaaatggc aggtcagcca cggcccgttc gaaagctacg
    22801 atcatctcgg tgaaaccaac tggtcattac tggtacaggc agtaaaccac tggcatgagc
    22861 cgaccgccgc gctgatgcga ccgttccgtg aactaccgga ctggcgtatt gatgatctga
    22921 tgatttcttt ttctgtacct ggcggcggcg tcggcccgca tctcgatcag tacgacgtgt
    22981 ttatcattca gggtaccgga cgtcgtcgct ggcgagtggg cgaaaagctg caaatgaaac
    23041 agcactgccc acatccggat ctgttacagg tcgatccgtt cgaagccatc atcgatgaag
    23101 agctggagcc tggtgatatt ctttatattc cgccaggatt cccgcatgaa ggctacgcgc
    23161 tggaaaatgc gatgaactat tccgtgggtt ttcgcgcgcc aaatacgcgg gaattaatta
    23221 gcggatttgc cgattatgtg ctgcaacgtg aactgggcgg caactactac agcgatccgg
    23281 atgttccacc tcgtgctcat cctgcggatg ttctgccgca agagatggat aaactgcgtg
    23341 agatgatgct cgaattgatc aaccagccgg aacactttaa gcaatggttt ggcgagttta
    23401 tatcccagtc acgtcatgaa ctggatatcg cgccgccgga accgccttat cagccagatg
    23461 aaatctacga tgcgctgaaa caaggtgatg tgctggtgcg cctgggtggt ctgcgcgtat
    23521 tgcgcattgg cgacgacgtg tatgccaatg gtgagaagat cgattccccg caccgtccgg
    23581 cactggatgc actcgccagc aacattgcgc tgactgcgga gaattttggc gatgcgctgg
    23641 aagatccgtc attcctcgcg atgctcgcgg cgctggtcaa tagcgggtat tggttcttcg
    23701 aagggtaagt ttggatttaa gccggatgca gcatcacatg ccgcatccct tttcgcttac
    23761 ttccgttgcg ccgttaactc ggcaatacgg acgatcacct gcaccgcttt ttccatacct
    23821 tccagaatca caaactcatg cttaccatga tagttgtaac cgccagtgaa caggttcggg
    23881 caaggtaatc ccataaacga caactgcgcg ccgtcggtac cgccgcggat cggtttcagt
    23941 tccggttcaa tatcgcagtc acgcatcgcc tgctgggcga tatcgagaat atgcggatgc
    24001 tcaaccactt tctcgcgcat attgtagtaa ctgtcttcaa tcaccaattc aatgtagcaa
    24061 tcaggatgta accctttgcc cacttttttg gcgatctcca tcattttacg tttacgcgct
    24121 tcaaactgtt tacggtcgaa atcacggatg atgtagtgca tatcggcccg ttcaacggtg
    24181 cctttcatgc tcgccaggtg atagaaacct tcatagcctt ctgtcatttc cgggctttca
    24241 tccgccggaa cttccgcatg aatacgtgcc gccagcgaca gcgcatttac catcactcct
    24301 ttcgccgtgc ccggatgaac attgttaccg acaattttta tattgaccga tgcggcgttg
    24361 aagttttcaa actccagttc gcctacgcca ccaccgtcaa cagtgtaagc ccagcgggca
    24421 tcgaaggcat caacatcaaa atgtttcgcc cctttgccca cttcttcatc cggggtaaag
    24481 gcgacgcgaa tatcaccatg cggaatgttt ttctgttgca ataccgccag cgcggtcatg
    24541 atttctgcaa tacctgcttt gtcatcagca cctaacaagg ttttaccatc ggtggtaatc
    24601 aacgtctgac ccagtagctg atgcagcacc gggaacataa ccggtgataa aacttcatcg
    24661 ccgataccca gcgcaatatc gccaccgcga tagttttcaa caatttgcgg attaacattt
    24721 ttgccgctgc aatccggtga ggtatccaca tgagaaataa agccaatcgc cgggatatcg
    24781 ccagggacgt tagccggtaa cgtcgccatc aaagtgccct tctcacttaa ggtcacattg
    24841 ataagcccca tttcttcgag ctgctctttc agcagatgca ataacttcca ttggccttcc
    24901 gtgctgggaa cctgtctcac ccctgctttt gattgggtat ccagagacac gtagttcaaa
    24961 aatcgctcaa gtagtttatc catgtagtca ccctcacttt ttgtgacaac attattaaga
    25021 agcaagaaaa gacaaatatt gcgtcaggtc acttttaccc ctgcaagcgg gaatatttat
    25081 cagcattacc ttaatgaata taaagctaag ccagtaattc acaacaaaga ttggcgattc
    25141 aagcggtttg ccgtagaatt accgccctca ttaggagtca ccaaggtggt taaccacaaa
    25201 ccccgcatcg gtaagccatc cgttgcgttt acatgggaca gagtaaaaaa ttgaataaac
    25261 aaccgagttc gctttcaccg ctggtgcaat tggcgggaat tcgcaaatgc tttgatggta
    25321 aagaggtcat tccccagctg gatctgacta tcaacaatgg cgagttcctc acgctgcttg
    25381 gcccttctgg ctgcggtaaa acaaccgttc ttcgcctgat tgcaggtctg gaaactgttg
    25441 attccggacg catcatgctg gataacgagg acatcaccca cgttccggcg gaaaaccgct
    25501 atgtgaacac tgttttccaa agctatgcac ttttccccca catgaccgtg ttcgaaaatg
    25561 tggcctttgg gttgcgcatg caaaaaaccc ccgctgctga aattacgccc cgcgtgatgg
    25621 aagccctgcg gatggtgcag ctggaaacct tcgctcaacg taaaccgcat cagctctctg
    25681 gtggtcaaca gcaacgcgtc gccattgctc gagcggtggt taacaagcct cgtctgttgt
    25741 tgctggatga gtcgctctca gcgctggatt acaaactgcg taagcaaatg cagaacgagc
    25801 tgaaagcgtt acagcgtaag cttggcatta cattcgtttt cgtgactcac gaccaggaag
    25861 aagcactcac catgtcggac aggattgtgg tgatgcgcga tggtcgcatt gagcaagacg
    25921 gcacgccgcg tgaaatctac gaagagccga aaaacctgtt tgttgccggg ttcattggcg
    25981 aaattaatat gtttaacgcc acggtgatcg agcgactcga cgagcagcgc gtacgcgcca
    26041 acgttgaagg ccgcgaatgt aatatctacg ttaacttcgc cgttgaaccg gggcaaaaac
    26101 tgcatgttct gctgcgcccg gaagacttac gtgttgaaga gattaacgac gacaaccacg
    26161 ctgaagggct gattggttac gttcgtgagc gtaactacaa aggcatgacg ctggagtcgg
    26221 ttgttgaact ggaaaatggc aagatggtga tggtcagcga attcttcaat gaagacgatc
    26281 ctgactttga ccactctctc gaccaaaaaa tggccattaa ttgggtagaa agctgggagg
    26341 tcgtactggc tgatgaagaa cacaagtaag ttccagaatg tagtgattgt cactattgtc
    26401 ggttggcttg tgttgtttgt ctttctgccc aacctgatga tcattggcac cagctttttg
    26461 acccgcgacg acgccagttt cgtcaaaatg gtctttacac tggataacta cacgcgtctg
    26521 ctcgatccgc tctattttga agtgctattg cactcgctga atatggcgct gatcgccacc
    26581 ctcgcctgcc tggtgctggg ctatccattt gcctggtttc tggcgaagct gccacacaag
    26641 gtgcgtccgc tgctgctgtt tctgctgatt gttccgttct ggaccaactc attgattcgt
    26701 atctacgggc tgaaaatttt cctcagcacc aaaggctatc tcaacgagtt tttgctctgg
    26761 ctgggcgtta tcgacacacc aatccgtatc atgttcacgc ccagtgcggt gattatcggt
    26821 ctggtttaca ttctgctgcc gtttatggtg atgccgctgt actccagtat cgaaaaactg
    26881 gataagccgt tgcttgaggc ggcgcgcgat ctcggtgcca gcaagttaca gacttttatc
    26941 cgcatcatta ttccactgac gatgccgggg attattgccg gatgtctgct ggtgatgctg
    27001 ccagcgatgg gcctgttcta tgtgtccgac ctgatgggcg gtgcgaaaaa cctgctgatc
    27061 ggtaacgtca tcaaggtaca gttcctcaat attcgtgact ggccgtttgg tgcagccacc
    27121 agcattacgc tgactatcgt aatgggcctg atgttgctgg tttactggcg cgcttctcgt
    27181 ttgctgaata agaaggtgga actcgaatga tcggtcgact gcttcgcggc ggttttatga
    27241 ccgctatcta cgcgtacctg tatatcccaa tcattatttt gattgtgaac tcctttaaca
    27301 gctcgcgctt tggcatcaac tggcagggtt ttaccaccaa atggtatagc ctgctgatga
    27361 acaacgacag cctgttacag gcagcgcagc attcactaac aatggcggtg ttttcggcga
    27421 cgtttgctac gcttatcggt tcactgacgg cagttgcgct gtaccgttat cgctttcgcg
    27481 gtaagccgtt cgttagcgga atgctgtttg tggtaatgat gtcaccagat atcgtgatgg
    27541 caatttcgct gctggtgctg tttatgctgc tgggtattca gcttggcttc tggtcgctgc
    27601 tgttctcgca tatcaccttc tgcctgccat ttgtggtggt gacggtgtat tcgcgcctga
    27661 aaggttttga cgtgcggatg ctggaagcgg cgaaagatct cggtgccagc gaatttacca
    27721 ttctgcgaaa aatcattctg ccactggcaa tgccagcggt ggcggcgggc tgggtgttaa
    27781 gctttaccct gtcgatggac gatgtggtag tttcttcatt cgtcaccgga ccaagttatg
    27841 aaattctgcc gttaaaaatt tattcgatgg tcaaagtcgg cgtatcgccg gaagttaacg
    27901 cgctggcaac catattactg gtgctgtcgc tggtgatggt tattgccagc cagcttattg
    27961 ctcgtgataa aacgaaaggt aacacagggg acgttaaatg aaaaaatggt cacgccacct
    28021 gctcgcggcg ggtgctctgg cactgggcat gagcgccgct cacgccgatg acaacaacac
    28081 gctgtatttc tacaactgga ccgagtacgt gccgccagga ctgcttgaac agttcaccaa
    28141 agaaaccggt attaaggtta tctattcgac ttacgagtcg aacgaaacca tgtacgccaa
    28201 gctgaaaacg tacaaagacg gtgcctatga cctggtggtt ccttcaacct attacgtcga
    28261 taaaatgcgt aaagagggga tgatccagaa gatcgacaag tcgaagttaa cgaacttcag
    28321 caatctcgat ccagacatgc tcaacaagcc ttttgacccg aataacgact actccattcc
    28381 gtatatctgg ggtgcgacgg cgattggtgt taacggtgat gcggtggatc cgaaatctgt
    28441 caccagctgg gccgatctgt ggaagccaga gtacaaaggc agcctgctgt tgaccgacga
    28501 tgcccgtgaa gtgttccaga tggcgctgcg taagctgggc tactccggta acaccaccga
    28561 tccgaaagag attgaagctg catataacga gctgaaaaaa ctgatgccaa acgtcgcagc
    28621 gtttaactcc gataacccgg ctaacccgta catggaaggc gaagttaacc tcggcatgat
    28681 ctggaacggt tctgctttcg ttgcacgcca ggcgggtacg cctattgacg tggtgtggcc
    28741 gaaagaaggc ggcatattct ggatggacag cctggcgatc ccggcaaatg ccaaaaacaa
    28801 agaaggcgcg ctgaaattga tcaacttcct gctgcgcccg gatgtggcaa aacaggttgc
    28861 tgaaactatc ggttatccaa cgccaaacct tgcggcgcgt aagctgttaa gtccagaagt
    28921 ggcaaacgat aagacgctct acccggatgc tgaaaccatt aaaaatggcg aatggcagaa
    28981 tgacgttggc gcagccagca gcatttatga agagtattat cagaagctga aagcaggacg
    29041 ttaattcagc aagcgggcgg tggtattacc gcccgttttt tagttagcaa ggatgtggct
    29101 aatgagtcag gacagtaaag ttttatcccg ctctttcgtc ggtattgtgg tccgcttgta
    29161 ggcctgataa gcgtagcgca tcaggcaatg cttcctgctt tataatccct ccagcaactt
    29221 ttcaacaaat tctggcacca cctggcttgc cgggccgtaa tatttctcgg caaattcatt
    29281 accaacctga ctcggttcaa gattcagttc aacggtgtga gcaccatgta gtttggcttc
    29341 gtgtacaaac ccagccgccg gatagacatg cccggaagta ccaatggcaa taaagacatc
    29401 cgccatcgac aacgccatat aaatttcatc catgccgagt ggcatttcgc caaaccaaac
    29461 gacgtgcggg cgcaagggtg ccgggaactg gcaacaatgg catttatctt ctggcgtaac
    29521 gtctccggtc cagtcgagaa cctgaccact ttgcgaacaa cgaactttca gcaattcccc
    29581 atgcatatga atgacattgg tattacctgc gcgttcatgc aggttgtcta tattctgcgt
    29641 taccagcaaa aagcgatcgc cgagagcgtc ttgcagttta gccagcgcaa gatgcgcggc
    29701 gttaggctga atttctggct gctgcagctg tcgacgacgg gcgttataaa acgcttgcac
    29761 cagttcagga tcgcgatcga aaccttccgg cgttgccaca tcttcaaccc gatgttcttc
    29821 ccacaggcca tctgcggcgc gaaaggtacg aatacctgat tccgcagaaa ttcctgcccc
    29881 tgtcagtacg agtactcttg gtttttccat tgcttccggc accactttat ctctgaaaaa
    29941 aatacgctga cgcaaacgct cgcgcaggcg acgtttattt ttacgaaaac gacttaaccg
    30001 atgaccccga cgcgacagca tagcaacctc tgttgtttaa tcggttagat gtaggaaggc
    30061 cgcaccacgc attcctcccg catcaccgtg gcgcgcgcgt tcaatgcgtg gaacacgagc
    30121 tacaggtaag agatgacaag gcaacctgtc cgccagttgc gttgtgattg ccgggaaatt
    30181 cgataagcca ccaccaatga cgaccaggtc agggtcaaca atggtcagga tatttcccag
    30241 agaaaccgct aataaatcca gataacgctc aacgtgcgcc cttgcctgct catcgccttg
    30301 atcataaagc gcaataattt cgggagcctg caacggttga tgataatagt gttgatacag
    30361 ccacgcaaaa ccgcgaccag acagataatt ttcaatgcag ccatgctgac cacagccgca
    30421 gcggcgtaac gggaaatcca accccatcat ggttaacgca tcaaccggca gacgcatatg
    30481 gccaaactcg ccggtaatgt agcttttacc agtaatcggt ttgccgttga aaaccagccc
    30541 gccgccaacg ccggtgccga gaattaaccc catcaccagt ggatattgcg taaattcatc
    30601 atcccaggct tctgaaaggg caaaacagtt ggcatcgtta tcaaggcgta catcgcgatc
    30661 aagacgtgcg ctcaggtcgg cacgcagcgg tttgccgctg gcagcgggga cattagcggc
    30721 atacagctta ccatcttctg tttccggcat tcccggaata ccgatgccga cagaaccttt
    30781 acagccaaaa cgttggtcag cttcagctac cagctcacac actgcatcta aaaatgcgtc
    30841 atagctgtca cgcggtgtcg gcacccgctt ttcccactgc aactgccgac cgctatcaaa
    30901 cacgccaagc gcaatttttg ttccaccaat atcaaacccg taatacattg ccgctccttg
    30961 ttttaatgta ctgcctttac tggccgctaa ggactcgcgc agggtcaata ttactggcgc
    31021 gccgcgccgg ataccaactt gccaaaagac tcagcaacaa tgctgtgacc agtacgtaga
    31081 agacgtccag ccagtgcaat tccgatggca ggaagtcaat aaaataaata tcgctggaga
    31141 ggaactgatg accgatcagc ttttcaatcc actcaataat cggggtaagt tgcagtgaaa
    31201 caactacgcc gataaccacc ccacacaggc tgccgaacag ccctgccagc aatccatacc
    31261 agacaaagat ggcgcgaatt aaaccatctt tcgcccccag cgttcttaat actgcgatat
    31321 cgccactctt gtctttcacc gccatcacta aggtggagac gatgttgaaa caggccacgc
    31381 caatcaccag taccatcgcc agatacataa tggcgcggat catctggata tcgcgataca
    31441 tatagccgta agtaccaatc caacttttaa tataaacata gctgttggtc acttcacccg
    31501 catcgcgtac cagcttattg gcgttgaaaa catccgtcat tttaagggca atacctgaca
    31561 cgctggaacc catatcaaga tattgttggg catccgccag cgggatcatg gcaaaactgt
    31621 gatcgagttg accactcaac tgcaaaatac cggcaatgtg caaacgcaca cgttttggct
    31681 gcatcagttt atgctcggga ttcgagttgg ggatcatgat cgacacccaa tcgccctgct
    31741 tcactttcag cgcatccgcc acgcctttgc cgatgataat ttgctgttcg cccgctttaa
    31801 aattgcgcca cgcatccccc tgaacaaacg agggtaatgc gctcagacgc tgttcctgtt
    31861 gtgggttaac gcccttcacc tggattgcgc gcagattcgc tccactttcc accagaccgg
    31921 tgaaattgat atacggcgca gcggcggcaa tacccggcac tttctgcacg ttatccagtg
    31981 cttcctgcca gttagtccat ggctgattta ccgcttcgat ttcgccatgc ggcaccaccg
    32041 ccagaatgcg gttattcagt tcgcgttcaa agccgttcat cgcgcttaag ccgacgatca
    32101 ataccgccac gccgagggca atgccaatgg tagaaatgac agagattagc gacaccatgc
    32161 cgccgcgtcg ccgtccgcga ctaaaacgca ggccaatcaa taacgataaa ggcatcgcca
    32221 ttactccgcc cccatcaggc tcagttccgc cgtcagacgt ccatcacgca tttccagttg
    32281 gcggctcata cgtttcgcca gttgcaggtc gtgagtgacc accaggaaag cggtgccctg
    32341 caagcgattc aactccccaa gcaactggaa gatgctgtcg gcgttacgcg catcgaggtt
    32401 accggtaggt tcatccgcca gtaccagacg cggattattg accagcgcac gggcaatcgc
    32461 tacacgctgg cgttcgccgc cagaaagttc agatgggcgg tgattcgcac gatgctccag
    32521 ccccaccgct tttaacatct ccagtgcaca gctgttgatt tcagcgggct ttttcttgcc
    32581 aatcagcagt ggcatagcca cgttttccag ggcagtaaaa tccggcagca ggtggtgaaa
    32641 ctgataaata aagcccagct tctggttgcg cagttctgct ttcgccgctg aagacagttt
    32701 gctcatcggc tgaccgttaa agatcacatc gccggaggtt ggcgtatcca gcccgcctaa
    32761 caggtgcagc aaggtacttt taccggaacc agagctaccg acaattgcca tcatttcacc
    32821 ttcgcccacg ctgaaactga cattgagcag cacatcggtt tgcacactgc cttcctgata
    32881 gcgtttgcac aggttgtcgc attgcaacag gatcttattc ataacgtaaa gcctcagcgg
    32941 gttgtgtggc ggcagcgcgc catgaagggt aaagcgtaga cagcagcgcg atagccatcg
    33001 ccaccagcgc aataacaatg acctgtaaag gttcgatagc caccggcagc gccgcgccat
    33061 caagcaggac gccgattatc ggcatcagat tatttaactg gctggcaagc agtgcgccaa
    33121 gcgccgctcc gaggattgca ccgataatcc cggcgctagc cccttgcacc ataaagacca
    33181 tcatgatttg tcgcggagtt aagccttgcg tttgcaggat cgcgacttcg ccctgcttct
    33241 ccatcaccat cagccccagc gaggtaataa tgttaaacgc cgcaacagcg acaatcaggc
    33301 tcagcagtaa gcccatcata tttttttcca tgcgtacggc ctggaacagc tcgcctttac
    33361 ggtcacgcca gtcctgccat ttgctgcctt caggcagttt ttgctgactt aaagagtcaa
    33421 ctctcagcgg ctcatccagc cacaaacgcc agccggtaat attggctgcc ggataacgca
    33481 tcagacgcga ggcatcctca atattcacca gcatttcata gccatcgact tcactgttgg
    33541 cggcgaaagt gccaatcaca ttgaacaggc gctgacttgg aatacgcccc atcggcgtga
    33601 actggctggc agatggcacc atcacgcgga tttgatcacc gcgattgacg cccaactgtg
    33661 aggcaagttg ttcgccgagg atgacattat atttccccgg ctcgaggtca gtttgtttca
    33721 cattgaccag atacggcgtt agtggatctt tttgcgccgg gtcgataccg agcatcaccc
    33781 cgaccgccac gctgcgcgcg ctttgcagta ccacatcacc ggtagtaata ggtgcgacgc
    33841 gattaacgcc gtccagtttg actgccgttt ccgggagttg ctgcgggtta agagagccat
    33901 gctcagaaga gagaattgcc tgtggcatca ggccaaggat gttgttttgc agctcgcgct
    33961 caaagccgtt catcactgac aatactgtga ccagcgccat caccccgagg gtaatgccga
    34021 tggtagaaag ccaggagacg aaacgaccga agcgatccac tgcacgccca cgcatgtaac
    34081 gcaggccaat aaatagagcg acaggttggt acatgaaatc cgtctggttg ctgtagcaaa
    34141 gacacgagta tataagcgaa agcaagatgg gtaaacgtct gaactgtaag tgatcgtcag
    34201 ggctaatctg aaaagccccc gtaaatcaat cggctaaatt gccatatttt atcatcaatc
    34261 ttgccgcctg atcttaatct gaaaaatcat ccaatacaga cagataccca ttacacctcg
    34321 ggtcataacc agcaggatca accgcatgta aattaaaaaa gagcgtgttg ttctgatgaa
    34381 acaaaaagag ctatggatta accagatcaa agggttatgt atttgtctgg tggtgattta
    34441 tcactcggtc attacctttt atccgcatat gaccactttt cagcatccgt tatcggaagt
    34501 cctgagcaaa tgctggatct atttcaatct ttaccttgcc ccctttcgta tgccggtttt
    34561 tttctttata tccggctatt tgattcgccg ctatatcgac agcgtaccgt ggggaaattg
    34621 tctcgataaa cggatctgga acatcttctg ggtgctggcg ctttggggcg tggtgcagtg
    34681 gctggcgtta agtgcgctga accagtggct ggcacctgag cgcgatttaa gcaatgcctc
    34741 caatgccgct tatgccgatt ccaccggtga gttcctgcac gggatgatca ccgccagcac
    34801 cagcttgtgg tatctgtatg cgttaattgt ctatttcgtg gtatgtaaaa tttttagccg
    34861 tctggcgctg ccactattcg ccttgtttgt actgctgagt gtggcggtta atttcgttcc
    34921 cacgccgtgg tggggaatga acagtgtgat acgcaatttg ccttattaca gccttggcgc
    34981 atggtttggc gcaacaataa tgacctgtgt taaagaggtg ccgttgcgcc gccatctgct
    35041 gatggcttct ttgcttaccg ttctggcggt cggtgcctgg ttgtttacta tctcgctgct
    35101 gttgtcgctg gtatcgattg tggtgatcat gaagctgttt tatcagtacg agcaacgatt
    35161 cggtatgcgc tccaccagcc tgctgaatgt gattggctcc aacaccattg ctatctacac
    35221 cacccatcgc attctggttg aaatattcag cttaactctg cttgcgcaaa tgaacgcagc
    35281 acgctggtcg ccgcaagtcg aactgacact cctgctggtt tatccctttg tcagtttgtt
    35341 catctgtact gttgcgggct tgctggtaag aaaactttca cagcgtgcat tcagcgatct
    35401 gttgttctcc ccgccttcac tgcccgctgc cgtcagttac tctcgttaaa ccatcacccc
    35461 agccggggcg attcaatttg ctaaaccatg tcgattacgg ataatgaaca gcattgctta
    35521 tcagagatat gcccccatat gttgaggcat atcctaacga gaatctgaca accgttatgc
    35581 ctgaacaata tcgttatacg ctgcccgtca aagcgggtga gcagcgtctg ctgggcgagt
    35641 taaccggcgc agcctgtgca acgctggtag cggaaattgc cgaacgtcac gccggtccgg
    35701 tggtactcat tgcaccagat atgcaaaatg ctctgcgttt gcatgatgaa atcagccagt
    35761 tcaccgatca gatggtgatg aatctggcgg actgggaaac tcttccctac gacagttttt
    35821 cgcctcatca ggacattatc tcctcgcgcc tttccaccct ttaccagcta ccgacgatgc
    35881 agcgtggcat actgattgtt ccggtgaata cgcttatgca gcgcgtttgc ccacacagtt
    35941 ttctccacgg tcatgcgctg gtgatgaaaa aaggtcagcg cctgtcacga gatgcattac
    36001 gaacccaact ggacagcgcc ggttatcgcc atgttgacca ggtgatggag cacggcgaat
    36061 acgccacgcg cggcgcgttg ctggatctct tcccgatggg gagtgagctg ccttatcgtc
    36121 ttgatttctt tgatgatgaa atcgacagcc tgcgggtgtt tgacgtcgac agccagcgca
    36181 cgctggagga agtagaagcg atcaatctgc tgcccgcgca cgaatttccg accgataaag
    36241 cggcaattga actgttccgc agccagtggc gcgatacctt cgaagtgaag cgcgatccgg
    36301 aacatattta ccagcaagtg agtaaaggca cattacctgc tgggatcgag tactggcagc
    36361 cattgttctt cagcgaacca ctgccaccgc tgttcagtta tttccctgcc aataccttgc
    36421 tcgtgaatac tggcgatctg gaaaccagtg ccgaacgttt ccaggctgac acgctggcgc
    36481 gttttgagaa tcgcggcgtc gatccgatgc gcccgctgtt gccaccacaa tcgctctggc
    36541 tgcgggtgga cgagctcttc tcagagctga aaaactggcc ccgagtgcag ctaaaaactg
    36601 aacatttacc gacaaaagcc gcgaatgcca atttaggttt ccagaaactg ccagacctgg
    36661 ccgttcaggc gcaacaaaaa gcgccgctgg atgcgctgcg taagttcctc gagactttcg
    36721 acggtccggt ggtgttctcg gtagaaagtg aaggtcgccg tgaagcgctg ggtgaactgc
    36781 tcgcacgaat taaaattgct ccgcaacgca ttatgcgtct tgatgaagcc agcgaccgtg
    36841 ggcgttatct gatgattggc gctgccgaac atggttttgt cgataaggcg cgtaatctgg
    36901 cgctgatctg cgaaagcgat ctgctcggtg aacgtgttgc ccgccgtcgt caggattctc
    36961 gccgcaccat caaccccgat acactgatcc gtaaccttgc ggagctgcat attggtcagc
    37021 cggtggtcca tctggagcac ggcgtcggtc gctatgccgg aatgaccacg ctggaagctg
    37081 gcggcattac tggcgagtac ctgatgctca cctatgccaa cgacgccaaa ctgtatgttc
    37141 cggtgtcgtc actgcatctg attagccgtt acgcaggtgg cgcggaagaa aacgccccgc
    37201 tgcataaact tggcggcgat gcgtggtcac gcgcgcggca gaaagcggcg gaaaaagtgc
    37261 gtgatgtggc ggcggaattg ctggatatct acgcgcaacg cgccgccaaa gagggcttcg
    37321 cgtttaaaca cgatcgtgag cagtatcagt tattctgcga cagcttcccg tttgaaacca
    37381 cgccggatca ggcgcaggcc attaatgcgg tacttagcga catgtgtcag ccgctggcaa
    37441 tggatcgtct ggtgtgcggc gatgttggct ttggtaaaac agaagtggcg atgcgcgccg
    37501 ctttcctggc agtagataac cacaagcagg tagcggtgtt ggtgcctacc acccttcttg
    37561 cgcagcagca ttacgacaac ttccgcgacc gtttcgccaa ctggccggta cgtatcgaaa
    37621 tgatctcccg tttccgcagc gccaaagagc agacgcaaat ccttgcggaa gtggcggaag
    37681 ggaaaatcga tattctgatc ggtacgcaca aactgctgca aagtgacgtc aagtttaaag
    37741 atttaggcct gctgattgtc gatgaagaac accgcttcgg ggtgcgtcat aaagagcgca
    37801 ttaaagcgat gcgcgcgaac gtggatattc tgacgcttac tgcaacgccg atcccacgta
    37861 cgctgaatat ggcaatgagc ggaatgcgtg acctgtcgat tatcgccacg ccgcccgccc
    37921 gtcgtctggc agttaaaacc tttgtccgtg agtatgacag cctggtggtc cgggaggcga
    37981 tcctgcgtga aattttgcgc ggaggacagg tttattatct ctacaatgat gtggaaaaca
    38041 ttcagaaagc cgccgaacgg ctggcagaac tggtgcctga agcacggatt gccatcggtc
    38101 acgggcagat gcgcgagcgc gaactggaac gggtgatgaa tgatttccat catcaacgtt
    38161 tcaacgtgct ggtttgtacc accattatcg aaaccgggat cgacatcccg acagccaaca
    38221 ccattatcat tgaacgtgcg gatcacttcg gtctggcgca gctgcaccag ttacgcggtc
    38281 gcgtcggacg ttcacatcat caggcatatg catggctgct gacgccgcat ccaaaagcga
    38341 tgactaccga tgcacaaaaa cgtcttgaag cgattgcctc gctggaagat ctcggggcag
    38401 gttttgcgct ggcaacgcac gatctggaga ttcgcggcgc gggtgaactg cttggcgaag
    38461 aacaaagcgg ctcaatggaa accatcggtt tctcgctgta tatggagttg ctggaaaacg
    38521 ccgtcgatgc actgaaagcc ggacgcgagc cgtcgctgga agatctcacc agccagcaaa
    38581 cagaagtcga gctgcggatg ccgtcgctat tgccagatga tttcatccct gacgtgaaca
    38641 cgcgtctgtc gttctacaaa cgtattgcca gcgccaaaac ggaaaacgaa ctggaagaga
    38701 tcaaagtcga gcttatcgat cgcttcggcc tgctgccaga tccggcgcgt accctgctgg
    38761 atattgcccg tctgcgccag caagcgcaga aactggggat caggaagctg gaaggtaatg
    38821 agaaaggcgg ggtgatcgaa tttgccgaga agaatcacgt taatccggcc tggttgattg
    38881 gtttgctgca aaaacagccg cagcattacc gccttgatgg tccgacgcgc ctgaaattta
    38941 ttcaggattt gagtgagcgg aaaacgcgta tcgaatgggt acgccagttt atgcgtgaac
    39001 tggaagagaa cgcgatcgct taatgtaaat cgggggcgtt tctgcgcccc catctgcaac
    39061 atttacaaat tctttgcact ttcctgcact atccgacact gtcaccatcc ataattcagg
    39121 cttatctgtt tattacaata accttatatt tattatgtat ttttggtgat gatcaaaacg
    39181 cgtttttctc gctggctaac gttttttacg ttcgccgctg ccgtggcgct ggcgctaccg
    39241 gcaaaagcca acacctggcc gctgccgcca gcgagcagtc gtctggttgg cgaaaacaaa
    39301 tttcatgtgg tggaaaatga cggtggttct ctggaagcca tcgccaaaaa atataacgtc
    39361 ggctttctcg ctctgttaca agctaacccc ggcgttgatc cttacgtacc gcgcgcgggc
    39421 agcgtgttaa cgatcccgtt gcaaacccta cttccagacg cgccgcgcga aggcattgtg
    39481 atcaacattg cggagctgcg tctctattac tacccgccgg gtaaaaattc ggtaaccgtg
    39541 tatccaatag gtattggtca gttaggtggt gacacgctga caccgacaat ggtgaccacc
    39601 gtttcagaca aacgtgcaaa cccaacctgg acgccaacgg caaacatccg cgcccgttat
    39661 aaagcacagg gaattgagtt gcctgcggta gtgccggctg gaccggataa cccaatgggc
    39721 catcatgcga ttcgtctggc ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc
    39781 gatttcggca ttggcatgcg ggtaagttct ggctgtattc gtctgcggga tgacgatatc
    39841 aaaacactct ttagccaggt caccccaggc accaaagtga atatcatcaa cactccgata
    39901 aaagtctctg ccgaaccaaa cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag
    39961 aagattgatg acgatccgca gctgctgcca attacgctga atagcgcaat gcaatcattt
    40021 aaagatgcag cacaaactga cgctgaagtg atgcaacatg tgatggatgt ccgttccggg
    40081 atgccggtgg atgtccgccg tcatcaagtg agcccacaaa ctctgtaaat cctggataaa
    40141 aaaaatcccc gcgacacggc atgtcgcggg gaaaaaatca gcagtagcag gcattaatga
    40201 gggttaatgc ttatttataa attactgctg ttccatggag ggtattcgga ccggttacag
    40261 aagtaatacg gaaagatttt gcgcccatct catccgcttt ttgcgccagc tgctcttcca
    40321 gcgatcccag atttgtcccc gcgttagcac tgatagtacc gactttttgt tggccttctg
    40381 gcgttgactg aacttcgaca gccgcaaagt tggcaaatga catggagctt aaaatcgccg
    40441 cagcgatgag gtttttacgt ttttcataat agtggcctta tgcagatgaa tgacgtcgca
    40501 agaaattact taacgatcga taactaaatg atagatgtga tctggatcac atacaagata
    40561 ttttttataa caatcattaa ttaatttaaa aatctttaaa tttcagtcaa atagaaagca
    40621 attattttta tgggatctgg actggtgaac ggaggtgatt atttttataa taacctttca
    40681 ttcacagtca ccaggtacaa cggcatcatg gcaactgact caacacaatg tgtaaaaaaa
    40741 agccgtggcc gcccaaaagt gttcgacagg gatgccgcgc ttgataaggc catgaaattg
    40801 ttctggcaac acggttatga agcgacttct cttgcggacc tcgtcgaagc aaccggagcc
    40861 aaagcgccca cgctatacgc ggaatttacc aacaaagagg ggttatttcg tgccgttctc
    40921 gaccgctata tcgatcgttt tgccgctaag catgaagcac agctgttttg tgaagagaaa
    40981 agcgtggagt ctgcgctggc tgactatttt gctgccatcg ccaactgctt taccagcaaa
    41041 gacaccccgg caggctgctt catgatcaac aactgcacca ccctctcccc agattcagga
    41101 gatatagcca atacgttgaa atcacgccat gcgatgcaag agcgcacttt gcagcagttt
    41161 ttatgtcaac gacaagcgcg cggggaaatc ccgacccact gtgacgtgac acatctggca
    41221 gaattcctta attgtattat tcaggggatg tcgatcagcg cacgcgaagg tgcatcgctg
    41281 gaaaaactga tgcagattgc cggaacgact ttgcgtttat ggcccgaact ggtgaaataa
    41341 gtcgcaaaaa aggcccctca gcctgagcga atgaggggcc aaattgcaga gtacagcctt
    41401 gttatacttt gttattcaaa atcagttgcc cgttactatc tagcgggatc tgcgtacccg
    41461 gatcgcggtc catgcggatt ttgccctgct gatcgccaat cttataggtc acatcataac
    41521 cgagcatttt ttctgacttg tcatacaccg ttttacaacg ctgttgcgta gtcgtgtaag
    41581 tatcgctttc ctggagagag ccctggatct ggttaccggc atatccacca cccagcgccc
    41641 ccacaacagt ggcgacatct ttaccgcgac caccaccaaa ctgatgccct atcacgccac
    41701 cagcaacagc gccgagcacc gacccggtaa tgcgattttc atcctgcacc ggtcgacgat
    41761 gggtcactgt gacgttgcga cactcctgac gcggtgtttt aaccgtttcc ttgattgggg
    41821 ttgcagaaac aacctgagcg tattgcgggc cccgttcaaa cacgttcaga ctggccactg
    41881 ccgctacgcc caacgcagct gcgacaccaa tcccgatacc cgccaacatt gatttattca
    41941 cgggaacacc tccttctttg cagttatgcc aatcatgcaa gcgaaggaga aaagcagcta
    42001 tcagacagaa gatgaaaagc gcgggaatga ctggaaatac ttaaggattc ggaggagtct
    42061 gaaggaatgc ctgatgcgac gcttaacgcg tcttatcagg cctaccgctc gtaccatttg
    42121 tccagggcgg ataaggcgtt tataccacat ccgccagtgt acgtcgatta atgcaacttc
    42181 aaacgcggac ggataacgcg gttaatactc cccaccagca tcattaatcc ggttttaaag
    42241 taaccatgca gcgcaatctg atgcattcgg tatagcgaga tatatacaaa gcgcgcaatt
    42301 cgtccttcaa tcatcattga gccgcgcgtc aggttaccca tcaggctacc aacggtggag
    42361 aagttcgaca gcgataccag cgaaccatga tctttatact gataattttt cagcggctta
    42421 ccgttcatct gcgccagaat gttgttcatt gcgcaagtcg ccatctggtg tgcagcctga
    42481 gcacgcggcg gaacaaagcc cccttccgga cgcgggcatg acgcgcagtc gccaatagcg
    42541 taaatgtctg gatcgcgagt ggtttgcagc gtcggttcca ccaccagctg gttgatacgg
    42601 ttagtttcaa gaccaccgat atctttcagg aagtctggcg ctttgatccc ggctgcccat
    42661 accatcagat cagcctcaat atattcgcca tctttagtgt gcaggccgcc ttcatcagca
    42721 ctggtgacca tggtttgcgt cagcacgcga acgccaagtt tcgttagctc gttgtgggcc
    42781 gcagcagaga tacgtggcgg taatgcaggc aaaatacgtt ctcccgcttc taccagcgtt
    42841 acgttcaggg cttcgttggt caggcctttg taaccgtagc tgtgcagttg cttgatcgcg
    42901 ttgtgcaatt cagcggagag ttctacaccc gtcgcgccgc cgccgacaat cgcaatgttc
    42961 actttgccat ttgcgcccag gttggcagag tatttcagga acagattcag catctcctgg
    43021 tggaagcgac gcgcctggtg cgggttatcg aggaaaatgc agttctcttt gacgcctggc
    43081 gtattgaaat cgttagaggt gctacccagc gccatgacca gggtgtcata ggcgatttta
    43141 cgttccggaa ccaacagttc acctttctcg tcgcgtagtt ctgcaatagt gattgttttc
    43201 gcttcacgat caatatcaat gacggaaccc aactggaact ggaaaccatg attgcgcgca
    43261 tgggccagat agctcaacgc atcgacgcct tcatcaagcg agccagtcgc cacttcgtgc
    43321 agcagtggtt tccacaggtg gctgtggtta cgatcgacca gcgtaatttt ggcttttttc
    43381 ttacgtccca gcttatgccc cagctgtgtt gccatttcca gcccaccagc accgccgccg
    43441 acaatcacaa tctttttcaa tggcgtagtc aacgtgcccc ccttaaattt attaaccaat
    43501 tgttaattaa tggttattaa catagctctt aattaacaac aggttacagc aatgaaaatg
    43561 ttcttcgcta ttgagaataa catgaatggt gcattggtca taccaaaatt gatgttaatc
    43621 aagttttgtt gcttaaaaga taggcaactt taccggggga gatcaaaaaa tagagtttat
    43681 tgtttattat ttgagcaaat tcggaagggt aatatagggt gaagcgtata tcgagggcgt
    43741 ttggtcgtcc tgataagatg cgcagcatcg catcaggcat tgatgctcga ctgtcggatg
    43801 cggcgtggac gccttatccg acttacagaa ttcggagcgc atcaggtacc ggcaaggtta
    43861 cctgatgcga cgagggcatt tacccgaggg ttttgaacgc tttaatgcgc tgtaaatgcg
    43921 gggagatatt cttgaatttg tgcgtctgct cttcgtccca gacaatctcg taataatgat
    43981 gcaactcttc agatgtccgc tggctgttaa gtgcttcatc attacgcgac aaaataacca
    44041 gacaacgatc gcgattcttc tcgcggaagt tggtcacaca cttagtggca atatcggcat
    44101 actcttccgg gcgatcaatc ttgccttcca tgttctcata agggaacaaa ttagggttga
    44161 agatcacctg gcggatatcg caaagaaaac caatccgttc cgcccagtat ccgcccaggc
    44221 caacgccgca aattagcgga cgctcgtcaa cgttcagttg caacattttg tccacttctt
    44281 taagcagatg ctgcatatca tgtttcggat gccgcgtact gtagcttatc aagcgtacat
    44341 ccgggtcaat aaactgcaat tgtaagactt tctcgtggtt acccggactg ttagagtcaa
    44401 aaccgtgtaa atagataatc atcgcttcct cacataagcc agggttagtg acctactttc
    44461 tcttcctgcc agcgttcatg taactgattc agacgggtgc tgatcgcttt ccagcgagcc
    44521 gagtccatca gttcctgtcg cgaaaatgaa cctttatgat acaaacgtgt aacacgttct
    44581 gccttgatcg gtgacagatt atctaacacg ctgacggccc ctttacgatt attgcagacc
    44641 aggatcatat cgcaacccgc atccagtgat gcctgcccgc gttcggcata actgcccata
    44701 atcgcggcac cttccatcga taaatcgtca gagaaaatca cgccgtcaaa ccccagttcc
    44761 tgacgcaaaa cggttttcag ccagtaggga gaaccgctcg ccggacgcgg atcaacatca
    44821 ctgtagatca catgcgcagg cataatggcg tcgagtttat tttcgcggat taaggaactg
    44881 aagaccgaca tatctttagc gcgaatctcc gcttgtggac gtggatcgca cggtgtttct
    44941 ttgtgtgagt ctgccgttac tgcaccgtgt cctgggaagt gtttcccggt cgttttcatt
    45001 ccggcttcat gcataccatc aataaaccgg ctggcaattg ccagggcttt ttgtggatcg
    45061 gcatgataag aacgctcgcc aatcgccgcg ctgatatgcc cgacatccag cactggcgca
    45121 aagctgatat caatatccat agcgatcatt tcgctggcca tcagccaacc cgcctcttgc
    45181 gccagtttgc cgccctcttc cattcccaac agcgcagcaa aggattgtgc tgccggtaag
    45241 cgggtaaaac cttcgcgaaa acgctgcacg cgtccacctt cctgatctac cgccaccacc
    45301 agatgattgc gcgatgctgc gcggatctgg cgcaccagtt cacgtaactg ggcaggatca
    45361 tgatagttac gcgtaaagag aatcagccct cccaccagcg gatgcgccag tatttcacgc
    45421 tcttccgcgt ccagttcgta accttcgaca tccaacatta ctggacccac actgctctcc
    45481 ttattgtttt attaatagct gccgccaggt gtcatcggcc agcctgataa attgttgatc
    45541 gccggtttgt cgccagcggt actcaaacca ccctcctttg agcatcagca gccagggaaa
    45601 ccatcgcctg acctgacgcc ataattgcgc cggataaatc ttcgcgcgag tggcatagtc
    45661 attgaccaat tgccggtgct gttcagtatt ttccacccac accgccgcca gttccagcgc
    45721 gatatcacca tctccagcat actcccagtc gatgagtttt aaccctgatg cgctatgcac
    45781 taaatttccg gcgtggacat ccatatgcaa tggacttaag cgtaaaggtc gtggttcccg
    45841 cgctttgcgc agacgtttta acattcgcag ccaacccaca gtccgccgcg ccggatcgct
    45901 ttgctgccag tacagttcca gtaagggcaa cagcgttatt cgccagccaa aacgtggttg
    45961 ttgatgtaga taatacagca agcctgccag ttcgttggta tccggcaaat acgtttttac
    46021 cgcaccgggc agatagtcga ctaccatcca gtcacggaga tataaatgcg gcttcggtgc
    46081 aatgcttgcg ggtagttgtg ataaagcccg atactggcgc aagaacgcgg actgcggcgc
    46141 atcaggatcg tgcggctgac gcacaacaaa acgctggccc tgatgttcaa tgagaaaact
    46201 accgccacta agcccactat taaacgtcgt gacgggatga aactgcggga aaaagcgcga
    46261 cagcaattcg tcgcgcgtga tgggattatt gctgcgaaac ggcaccttta cctgaccaga
    46321 taatttcgcc cgtctgcacc agcatcagct gcatttgtag ggtcggagcg ttaacgttgc
    46381 cagaggcgca ggagtacagc acgtaatgag cgccgacatt gcgggcaatg cctatggctt
    46441 tactacgggt gcctaaactg tcctgcggcg acaaacctaa ctgttgctta gccatcgaca
    46501 gttgctgggc ggaaaccagg gtaaatttcc cgttattagc cagcgcattt cgcagcgttt
    46561 cggtcgcttc tgcggcattc agcgaaccgt tagtacggtt gttaacgcta tcaaccagca
    46621 ggacgctacc cgcagtcacc ccgtcagccc caagcatctt actgaccatc ggctgcattg
    46681 cgccattcca gtcataatgg cgaatatgcg gcgcaggcgg tgccgttcga tcttcgtgct
    46741 caattgggcc tggctgctgc gggatcgtcg gcaccgaggg cactgtgggg acaggctgtt
    46801 gtggctcggc tggttgttcc ggcgctggtt tcacttcttc taccggtgca ggttcacgtt
    46861 gccccacaca cccggcgaga aacatcgcca gcgcggtaat caaggcgtag cgactcattt
    46921 ttgtcatcaa gattcacccc tttacaaata tagataaagt ctgactttgt gcgcccccag
    46981 aaaattggcg ctgccgtaca gcgttaccgc cgaatgtgcg ggaatggtaa cgctgcgtgg
    47041 cctttccaga ggatgcatct ccagccctct ggcgtcatac cagtaaaaac gataatgaac
    47101 ggtaacgggt tcttgccttt cgttataaat cgttgaggat gctgaaggtt gaatatcaga
    47161 cgtcgaaagg acgggctttt ctgcactgat gcccgcagcc aataaagatg actccatcac
    47221 cagcgattgc tcatcattca ccggaatttc cggatgtgaa cgacagccca ccagcagtaa
    47281 caacaccaga gacaccagcc caaagcatcc ttttctcatc gttacagacc tttatgcgcc
    47341 agcattggtc ccagcggacg gccacccaac aagtgcatat ggatgtggta aacctcttgt
    47401 ccgccatggc ggttggtatt catgatcaga cgatagccat cttcggcaat accttcttgc
    47461 tcagcaattt ttgccgctac ggtgatcatg cgtcccagcg cctgctcatg ctcagctgac
    47521 acgtcgttca cagtcggaat gagaatattc ggaatgatca gaatatgcgt tggcgcttgc
    47581 ggcgaaatat cgcgaaacgc cgttaccaga tcatcctggt agacgatatc ggaggggatc
    47641 tcacgacgaa taattttgct gaatatagtt tcttctgcca cgacattttc ctttttcaaa
    47701 tagccatgcg ttgcgccatg ctacgccgcg ccacactgcg agtatagagt atggtcgagt
    47761 taaacctgct ctttcaatct taactcacga tttcttaagc aaaaaatcgt tttgctgccg
    47821 tatatatcgc cattattccc atttctgcct gcgaatgcgc tgaaaagaca ttctctgttt
    47881 ggtttacacg acaaatatga atgcgtatat ttctcatttg catttgcagc aaaattattc
    47941 gcacactaaa tataaccgct catcaccctc ctccggcatg agcctgacga caacataaac
    48001 caagagattt caaatgcttt caacacaatt taacagggat aatcaatatc aagtcatcac
    48061 caaaccgtca ctacttgccg gttgcatagc actggcacta ttaccttctg ccgcttttgc
    48121 tgcaccagcc actgaagaaa cggtgattgt tgagggttca gccacagctc cagatgatgg
    48181 cgaaaatgat tacagcgtaa cgtctacctc tgcgggtacc aaaatgcaga tgactcaaca
    48241 tgatattcct cagtcggtca ctattgttag ccagcagcgg atggaagatc agcagttaca
    48301 aacgctgggc gaagtgatgg aaaacacgct ggggatcagc aaaagtcagg cggattccga
    48361 tcgtgctctt tattattccc gcggattcca gatcgataac tatatggttg atggtatccc
    48421 cacctatttt gaatcgcgct ggaatctggg cgacgcactt tctgatatgg cactgtttga
    48481 acgcgtagaa gtagtgcgtg gcgcgacagg actcatgacc gggacgggta atccatctgc
    48541 ggcaattaat atggttcgaa aacacgcgac ctgtcgtgaa tttaaaggcg atgtctcggc
    48601 ggaatacggt agctggaaca aagaacggta tgtggcggat ttacaaagcc cactcaccga
    48661 agacggtaaa atccgcgcgc gaattgtcgg cggctaccag aataacgact catggctgga
    48721 ccgctacaat agtgaaaaga ccttcttctc gggcattgtc gatgctgatt taggcgatct
    48781 tacgacgctt tcagccggtt acgaatatca gcgcattgat gttaatagcc caacctgggg
    48841 cggtttaccg cgctggaata ctgatggcag cagcaacagt tacgatcgcg cacgcagtac
    48901 cgcacctgac tgggcgtaca acgataaaga gatcaacaag gtctttatga ccctgaagca
    48961 gcggtttgct gatacctggc aagcgacact gaatgccacc cactctgaag tcgaatttga
    49021 cagcaaaatg atgtatgtcg atgcctatgt aaacaaagcg gatggtatgc tggttgggcc
    49081 atacagtaat tatggacctg gctttgatca tgtcggcggc accggttgga acagtggcaa
    49141 acgtaaagtt gatgcgctgg atttgttcgc tgacggtagt tatgaattgt ttggtcgtca
    49201 gcacaatcta atgtttggtg gcagttacag caaacaaaac aatcgttcag ttcatgggcc
    49261 aacatcttcc cggatgaaat tggcagtttc tacaacttta atggcaattt cccacaagcc
    49321 gactggtcat cacagagcct ggcgcaggac gataccacac atatgaaatc gttatatgct
    49381 gccactcgtg tcacccttgc cgatccgctg catctgatcc tcggcgcacg ttataccaac
    49441 tggcgggttg atacgctgac ttacagcatg gagaaaaacc acaccacgcc ttacgctggt
    49501 ctggtgtttg acatcaatga caactggtcg acctacgcca gctatacctc tattttccag
    49561 ccgcaaaatg atcgtgacag ttcaggcaaa tatctggctc caatcaccgg taacaactac
    49621 gagctgggtc tgaaatcgga ctggatgaat agccgtctga ccaccacgtt agccatcttc
    49681 cgtattgagc aggataatgt cgctcagtcc accggtacac ctatccccgg cagcaacggc
    49741 gaaaccgcct ataaagcggt ggatgggaca gtcagtaaag gggtggaatt tgaactcaac
    49801 ggcgcaatta ccgacaactg gcagctgaca tttggcgcaa cgcgctatct tgcagaggat
    49861 aacgaaggaa acgccgttaa tcctaatctg ccacgcacca cggttaaaat gttcaccagc
    49921 tatcggttgc ctgtcatgcc agagttgaca gtcggcggtg gtgttaactg gcaaaatcgc
    49981 gtgtataccg acaccgtgac accgtatggc accttccgcg ccgagcaagg tagctacgcg
    50041 ctggtggatc tcttcacccg ctaccaggtg acgaaaaact tctcgttaca ggggaacgtc
    50101 aataacctgt tcgacaaaac ctacgatacc aacatggaag gttctatcgt ctacggcgca
    50161 ccgcgtaatt tcagcattac cggcacgtat caattctgaa taacacctgt aaaaaaggca
    50221 gccatctggc tgccttagtc tccccaacgt cttacggatt agtggttacg gatgtactca
    50281 tccatctcgg ttttcaggtt atcggattta gtaccgaaaa tagcctgaac accagaacca
    50341 gcaaccacta cgcccgctgc gcccagtttc ttcaggccag cctgatccac tttagacaca
    50401 tcagcaacgc tgacgcgcag acgggtaata catgcgtcga ggttagtaat gttttcttta
    50461 ccaccaaatg cagcaaccag agccggtgcc atttcgctgg tacctgttgc ttttgcatct
    50521 tcagtcgcgt cttcacgacc cggcgttttc agatccagtg ctttaatcag cacgcggaag
    50581 atggtgtagt aaacaatcgc ataaccgata ccgacgatcg gtaacagcca cagtttgctg
    50641 ctgttaccag acagaacgat gaagtcgatc agaccgtgcg agaacgacgt accgtcacgc
    50701 atccccagaa gaatacagat tgggaatgcc aggcctgcca gaatcgcgtg gatgatgtac
    50761 aggatcggcg caacgaacat gaagaagaac tcgatcggct cggtgatacc ggtcaggaac
    50821 gaggtcagcg ccgcggagat cataataccg cccactttcg cgcggttttc tggtttagca
    50881 gagtgccaga tagcaattgc ggcagctggc agaccgtaca ttttgaacag gaagccacca
    50941 gacagtttac ccgcagtcgg gtcacccgcc atataacgcg gaatgtcgcc gtggaaaacc
    51001 tgacctgctg cgttggtgta ttcaccaatc tgcatctgga aaggtacgtt ccagatgtgg
    51061 tgcagaccaa acggtaccag gcaacgttcg atgaaaccgt aaatgccaaa cgcaactacc
    51121 gggttctggt aagcagccca ctgagagaag gtctggattg cagaaccaat cggcggccaa
    51181 atgaaggaca gcacaacgcc agtaaagatg gcagccaggc cagaaatgat cggcacaaag
    51241 cgtttaccgg caaagaagcc aagatactca ggcagcttaa tacggtagaa acggttaaac
    51301 atgtacgctg caatcgcacc ggagataatc cctccgagta cgccagtatc cgccaagtgt
    51361 ttagaggcga tttcttcagc aggtaaatgc agtaccagtg gcgcaaccac ggccatggtt
    51421 ttaaccatga tgccataggc aacaactgcg gccagcgcgg atacgccatc gttattggta
    51481 aagccgaggg cgacaccgat cgcaaaaatc agtggcatgt ttgcaaagac ggaaccgcct
    51541 gcttctgcca taacatgcga tacaacggcg ggcagccagc tgaaattcgc ggaaccgacg
    51601 cccagcagaa tacctgcgat aggtagtacg gataccggca gcatcagcga tttaccgacc
    51661 ttttgcaggt tagcaaatgc attcttaaac ataattgaga gtgctcctga gtatgggtgc
    51721 tttttttacg ttctcacgcg tggcaagggg ggagagcctc gccgtgtaca gggcatctaa
    51781 gcgcccttta tttattacac agagtaaaat aattcagtgc caatttgttt gacggctatc
    51841 acgtttcaac ttcagatcat tggagaagtt gcacattttg acaaaacata tgtcacaata
    51901 tttcaaacgc ggttattcac cgcccaattt tcggcggtga actttactcg ttttagccat
    51961 taattacgag ctttaaaaaa actcattcaa cggatggatt gaaggcggga agcgtcgatg
    52021 tgaaacagac gggcgaagtt atcggtggtt acctgcgcca gttcttcaac ggcaacacct
    52081 ttcaacacag ccatgtattc tgcaacgtca cgaaccatcg caggttgatt ctcttttcct
    52141 cgatgcggta ccggcgcaag gtaaggtgag tcagtttcca ccagtaatcg atccaggggg
    52201 acataacgcg cagcatcgcg cagttgctcc gcattacgga aggtcacaat gccggaaaag
    52261 gagatgtaaa atccgagatc cagtaattta cccgccgttt ctctgtcctc tgtaaaacag
    52321 tgtagtacgc caccgcaatc cgtcactttt tcttcgcgca gaattgccag cgtatcggca
    52381 cgggcgtcac gggtatggac gataaccggc ttgttcagtt cacgaccaat ctggatatga
    52441 tggatgaagg actcttgctg acgtacttta gtttccggtg tgtaataata atccagcccg
    52501 gtttcaccca gcgctacaac accctcttct gccgccagac ggcgtaaatc ttctacatcg
    52561 tagggatcat tctggttgag tggatgcacg ccacaagaaa atacgacgtt gtcacgttcg
    52621 cctaccagat cccgcatatg taaataaccc ggtaatgttg tggcgactgc cagacaaaat
    52681 ttcacatcgc gtgcggcggc tttcgccaga acgtcatcca cgtccttatg caaagattca
    52741 taatccagac catcgagatg gcagtgtgag tcgactaaaa acatgatgtc tctcttaaag
    52801 atgaggaacc ggtaacacaa cgcccggttg caggtaatgc tcaatacgca gtaaaagatc
    52861 ggtgatgaga agctcgcggt tgatgcctgt gacagacatt aactgttcac gaatgtggca
    52921 aacatccccc agtatagcct gcaggcacga gggagaaaga tggtttgcca gttcggcgac
    52981 caggcccggc acatcaacat tggtcacctg cgcagcgcca tgatggcgtt ttagcgcatc
    53041 catcagcaac gttgccagcc agtgtaaacg cgccggagct tgttcatgat taagggccgc
    53101 cagcagcgaa taccagtcgc ccgatggcac gctatatgcc aacgcctgac acaatgtttc
    53161 acgagcctgc cagttatctc cctgaaacaa cgccagtgcc gcgccaggcg aaccggcgct
    53221 taagcgcaat gcggcaagta atgcatcctg tgacattgtc acttcgcgtg aaagccaagt
    53281 cacggcgtac tgttctggag gcggcgcaag gtaatgtaac cgacaacgac tacgtaatgt
    53341 tgccagtaaa cgttcaggct cgcgggtagc caggaaaaac caggtttctg ctggtggctc
    53401 ttcaagcgtt ttcagcaatg cgttagccgc ggcgtcggtc agtaaggcag catcggttac
    53461 ccagacaact ttcgcaccac ctaggcgtgc gtgctcattc agcttttcgg tgacctcacg
    53521 taccgcatca atgcccagcg tatttttccc tttttcggga gccagggtgt agtaatcggg
    53581 atgcgtgcca gcctgcatca actgacatcc acgacagtga ccgcaacttt tgtggccctg
    53641 cggttgttgg cagagtaaat aacggctcag ggcgtagatt aaagcatcat cgcccatgcc
    53701 cggtaacgcc tgaatgagta gcgcatggtg acctcttccg gcctgatagc tggctaccag
    53761 tttttcgaaa tcaggtcgta accatggata ccatctcatg cgtccaactc cttcacccag
    53821 tgggtcacgg tagtgcggat tgcatccatc acggcctcca gcggctgggt ggcatcaatg
    53881 gtatgaatac ttttatcttg tgcagccagt tccagatagc gggcgcgggt gcgattaaag
    53941 aaatcgaaag actcttgctc aatacgatcc agctcgccgc gcgcacgcgc gcgttttaag
    54001 ccaacttccg gggtaacatc gagatagagc gttaagtctg ggcgaaaatc cccgagaaca
    54061 gcatcacgca gtgttgccag catatgttgg tcaataccac gtcctccgcc ctgatacgcc
    54121 tgagtggaaa gatcgtggcg atcgccaatc acccaggtgc cgttagccag cgcaggtttg
    54181 atgaccgttt ctaccagttg aacgcgcgcg gcataaaaca tcagaacttc ggctttatcg
    54241 gtaatgactt catcgcctac cgatttgata tccagcacca ggcttcttaa cttttcggca
    54301 agttgcgtac cgccaggttc ccgagtgaaa accatgtcgc ggatacccag ttgctcgagc
    54361 gtctcaacca ccacattacg cgcggtagtt ttgcctgcgc cttccagccc ctcaatgacg
    54421 atatacttac tgcgcatttt tttccttaag cactttcaga taatcctgca cagacttgtt
    54481 atgactggca agattggtat taaacgtgtg accaccttta ccatcggcca caaaatagag
    54541 atacggcgtt tttgccggat gcgcagcagc cttcagcgaa tccgcccccg gcgtagctat
    54601 cgcacccggc ggcaaaccgg taatggtata ggtgttatac gctgtcggcg tttccaggtc
    54661 tgcacgagaa agtttgccat tataacgctc tcccatcccg taaatcacgg ttgggtcggt
    54721 ctgcaggcgc ataccaatgc gtaaacggtt gataaatacc gaggcaacct gatcgcgttc
    54781 actggcaacg gcggtttctt tttcgataat tgatgccatc gtcaccagct gatttttatc
    54841 tttataaggc agaccgtccg cacgcccttc ccaggcgcta tcgaccgctt tcaccatttt
    54901 cttgtgaact cgcttgagta acgcgacatc ggtggtattg gcggtataca tcaaggtgtc
    54961 tggccagaac caaccttcaa tccactccgg gttttccagt tcaagtgcct gcgctacggt
    55021 ggcgtactta tcgtcactca gcgtgtgctt gatatacgga gcctcacgca attgcttgag
    55081 gtagtcgctc agacgcatcc cttctaccag tcgcagcggg aactgtgctt ctttaccgct
    55141 ttccaacaat ttcagcatct cgcgcacggt catctgcggt gtaaagcggt aagtcccggc
    55201 tttaaagtga gaaagatccg gttcgatacg cagcagccat tgaaaaaccc gtgggcgatt
    55261 gatgatctta tcagcataaa gctgttcacc gagcgccaga cgtccggtcc ctggcttcag
    55321 ggtaaatatc gtctcttctt tgataagcaa tttgctgtcg gcaagatggc gaaccttcca
    55381 gacgcccaca ccagcggcga tacccagtac caccagcaat aacaagataa tcaataacac
    55441 ttttttcatg actaattcgg gcgctcacaa agtggggcta aatattcata taacgttgct
    55501 gacgaaaagg tgacatcgcc acaggcacgt acgggcatca ctggcattaa cgcattacaa
    55561 ataaccatct catctgcctg caacgcctcc tccagcgagg cttgcacttc gacaagctga
    55621 taagaggatt gtgccagtaa acggatacag aattgtcgca taatgccgtt aacacctgcc
    55681 tgatccagtc gcggcgtata aactacattg cccttccgcc agaacaaatt agccgcacag
    55741 cattccgtaa cccacccttc gctgtcaagg accagcgcct catcagcgtt tgtctgctca
    55801 agatgagagc gaatcaatac ttgctcaagc cgattaagat gtttaatacc tgcaagatga
    55861 ggattgcgcc ccagccgcac cgggcttagc gccaacgtca tcccttcgtt acgcaaacgg
    55921 tcgtaatgtg caggataagc cgtaacggag agaatccgcg ttgctggtcc gctgttcaat
    55981 gtgctgtacc ctcgcccgcc actaccgcga ctgatcacga ctttcagcac accattttgc
    56041 tgttctgctg ccagcgtttt catctcttgt tcaagctgag gccagaagtc acaggaaatc
    56101 atcaaccgct gacaagcatc ctgtagtcgc aggatatgcg ccgataacaa actgacttta
    56161 ccgtcgataa ctctggcggt ggtaaaacaa ccatcaccaa actgcgttgc ccgatcgctt
    56221 actgccagcg attcctgctt ataaccgtta attaagaaca taccggctcc ttatcagaga
    56281 attgttagtg tggcagcatg ttcactacgg aacaagtcgg aataaaagct aagaaaaaag
    56341 gcccgcaagc ggacctttta tatgggtgga aaatgacaac ttagatcttt ttaaagatca
    56401 aagaaccatt agtgccaccg aagccgaagg agttacacag cgtgtattcc attccgctga
    56461 cctgacgtgc ttcatgcggt acgaaatcca gatcgcaacc ttcatccggg ttatccaggt
    56521 tgatggttgg cggaacagcc tgatcgcgca gcgccaggat ggagtagata gattctactg
    56581 cacccgccgc acctaacagg tgaccggtca tagatttcgt ggagcttacc aacacacggc
    56641 ttgcagcttc accgaagatg gttttcaccg cctgcgcttc agctttatcg ccagccggcg
    56701 tagaagtacc gtgcgcgtta acgtagccaa tctgactcgc ttcaatgcct gcatcacgca
    56761 gagcatttgc cattgccaga gctgcgcctg cgccattttc tggcggtgac gtcatatgat
    56821 aagcatcgct gctcatacca aagccgacga gttcagcgta aattttcgca ccgcgttttt
    56881 tcgcgtgttc gtactcttca agtaccagca taccggcacc atcgcccagt acgaagccat
    56941 cacgctcttt atcccacggg cggctcgccg cttgcgggtt atcattgcgg gtagataatg
    57001 cacgtgccgc gccaaaacca ccaacgccca gcggcgtact ggctttctct gcgccacctg
    57061 caaccatcac gtcagcatcg ccatacgcga taatacgcgc agcatggcca atgttgtgca
    57121 cgccggaagt acaggcggtc gcgatagaga tgcttgggcc acgcaggcca tacatgatag
    57181 tcagatgacc tgccaccatg ttcacaatcg ttgacggaac gaagaatggg ctgatcttac
    57241 gtggaccacc gttcatcaga gatgtgtggt tttcttcgat cagtccgagg ccgccaatcc
    57301 cggagccaat tgcggcacca atgcgggttg cgttctcttc cgttatttca aggccagaat
    57361 cctgcatggc ctgaacgcca gcgacaattc catattgaat gaaggcatcc atcttgcgct
    57421 gttctttgcg cgagataatg tcctcacagt taaaatcctt tactaagcca gcaaatttcg
    57481 ttgcataggc gctagtatcg aaatggtcga ttaggctgat gccactctga ccggcaagca
    57541 gagctttcca ggtagactct acggtattgc cgacaggaga caacatgccc agtccggtca
    57601 caactacacg acgcttagac acgtttgtcc tccaaggagg gaaaaatgat tctagtggga
    57661 caaaaagata aaactcaggc ggtcgaacga ccgcctggag atgttcactt acgcctggtg
    57721 gccgttgatg taatcaatgg cagcctgaac ggtggtgatt ttctcagctt cttcgtccgg
    57781 aatctcagta tcaaactctt cttccagagc cattaccagc tcaacggtgt caagagaatc
    57841 cgcgcccagg tcttcaacga aagaagcatt gttggtaact tcttcctgct taacgcccag
    57901 ctgttcgccg ataattttct taacgcgttc ttcgatagtg ctcatactct taaatttcct
    57961 atcaaaactc gctttcgcga tggttttcgt agtgtataaa atgttgaaaa atttgcaact
    58021 aaatcccggc aggtcttacc acgattttac gttattttga ggccttaccc cccaataacg
    58081 caaatcattt tgcactaatt attactcata accacgcaga ctgcgcgcaa catgagcatt
    58141 ttgtgcaaat cgcggtcaga ccatgtacat cccgccgttc acatgcaaag tttcacccgt
    58201 gatgtaagct gcttcgtcgg atgccaggaa tgcaaccgcg ttggcgattt cctgtgcgcc
    58261 gccgaggcga cccgcaggaa cctgcgccag gatacccgca cgctggtcat cgctcagcgc
    58321 acgtgtcatg tccgtttcaa taaagcccgg agcaacaacg tttacagtaa taccgcgtga
    58381 cgcaacttcg cgcgccagtg atttactgaa gccgatcaag cccgctttcg ccgcagcgta
    58441 gttggcctga ccgccatttc ccatggtacc aaccacagaa ccgatagtga taatacgacc
    58501 atgacgcttt ttcatcatag cgcgcattac cgcttttgac agacggaaaa cagatgaaag
    58561 gttagtttcg ataatatcgt tccactcttc atctttcatt cgcattaaca ggttatcacg
    58621 agtgataccg gcattattga ccaggatatc cacttcacca aattctgcgc gaattttttc
    58681 cagaacagat tcgatagatg ccgggtcggt cacattcaac atcagacctt tgccgttggc
    58741 acctaaataa tcactgatcg cctgagcgcc attttcactg gtcgcagtgc caataacttt
    58801 cgcgccacgg gctgcgagcg tttcagcaat tgcgcggcca attccgcggc ttgcaccggt
    58861 taccagtgcg atttttcctt caaaattcat gattttcctc ttttaaagct cgagcgccgc
    58921 tgccatcgct gaaggttcgt tcagcgccga ggcggtcagg gtgtcgacaa tgcgtttcgt
    58981 caggccagta agcaccttgc ccgggccgac ttcatagaga tgttctacgc cttgcgctgc
    59041 catgtactca acagacttcg tccactgaac cgggttatac aactgacgta ccagtgcgtc
    59101 acggatggca tcaccattgg tttcgcattt cacatcaacg ttattcacaa cagaaactgt
    59161 tggtgcgtta aaggtgattt tcgctaattc tactgccagt ttgtcggctg ctggtttcat
    59221 cagcgcacag tgagacggta cgctcactgg taacggcagc gcgcgttttg cgccagcagc
    59281 tttacaggca gcgccagcac gctcaaccgc ttctttatga ccggcaataa ccacctgtcc
    59341 cggagagtta aagtttaccg gagaaacgac ctgaccttct gcagcttctt cacacgcttt
    59401 cgcaataggc gcatcatcca gaccgatgat tgccgccata gcgcccgtgc cttccggtac
    59461 ggcttcttgc atgaacttac cgcgcatctc aaccagacgc accgcatcag cgaaatcaat
    59521 cacgccagcg caaaccagcg cggagtattc ccccaggctg tgaccggcca tcattgccgg
    59581 tgctttaccg ccctgctgct gccatacgcg atacagcgca acagatgcag tcaacagcgc
    59641 tggctgggtt tgccaggttt tattcagttc ttcagctgac ccctgctggg tcagcgccca
    59701 caggtcgtag cccagcgccg cagaagcttc agcaaacgtt tcttcgacaa ttggatagct
    59761 cgccgccata tcagccagca ttccaacggt ttgagaaccc tgtccaggga acacaaatgc
    59821 aaattgcgtc atgttttaat ccttatccta gaaacgaacc agcgcagagc cccaagtgaa
    59881 tccaccgcca aaggcttcaa gcagaaccaa ctgccccggc ttaatgcgcc cgtcgcgtac
    59941 agcttcatcc agcgcgcacg ggacagaggc cgcagaggta ttaccgtggc gatccagcgt
    60001 caccacgaca ttgtccatcg acataccgag ttttttcgcc gttgcactga taatacgcag
    60061 gttagcctga tgcggaacca gccagtccag ttgagaacgg tcaagattat tcgccgccag
    60121 cgtctcatca acgatgtgcg ccagttccgt taccgcaacc ttgaagactt cgttgcccgc
    60181 catcgtcaga tgaattgaat tctctggatt cacgcggtcg gcgtttggca gcgtcagcaa
    60241 ttcaccataa ctaccgtcgg catgcagatg ggtggaaatg attcccggct cttcagaggc
    60301 agccagcacc gcagcgcccg cgccatcgcc aaaaataata atagtcccac gatctgttgg
    60361 atcgcaggtg cgcgccagta catcggaacc gacgaccaga gcatacttca ccgccccaga
    60421 tttcacgtat tgatcggcta cgcttaatgc ataggtgaaa cctgcacagg ctgctgcaac
    60481 gtcaaatgcc gggcaacctt taatgtccaa catgctttga atctgacaag ctgcgctcgg
    60541 gaaagcgtgc gtagcagaag tcgttgccac aacgatcagg ccaatctggt ctttctcaat
    60601 gcccgccatc tcaattgcgc gtgtcgccgc ttcaaagccc atggttgaaa cggtttcgtt
    60661 tggcgcggca atgtggcgtt cgcggatacc ggtacgagtg acaatccact cgtcagaggt
    60721 gtccaccatt ttttccaaat cggcgtttgt ccgcacttgt tcgggcagat agctgccagt
    60781 accaataatc ttcgtataca tgtacgctca gtcacttttc ggttatatac cgtcacttgc
    60841 aaactgcgag ttcgctggca gcgtcctgct accgcagagt tccgcttttg ccaccgtcca
    60901 gcagctcaaa accagctggg tatacagatt ccaggcgagc ggcaattcgc tgaggaactt
    60961 gtcgctgcac cgcctgcact gcctgttcaa tcgcgaccgc aaaagctcgc tgattggctg
    61021 caccatgact ttttatcacc gtgccgcgca atcctaacag acaggcgccg ttatactggt
    61081 cggggttgag gtgactgaat cgcctcgtca ggctcttttg tagccaacgc tttaataaca
    61141 gtagccacca cgaccgtttt ttcccttcac cctgagattt cagcagagaa aggaacatcc
    61201 tgacaacacc ttccatcgtc tttaacgtga catttcctgt aaagccgtca caaaccagca
    61261 catctgtctt gccagttaac aactcattgg cttcaagata gccgatataa ttgatagaag
    61321 ggattgtttt aagcaccgct gaggcatccc gaatactgtc aagacccttt acttcttctt
    61381 caccaatatt gagcaacgcc acgcgaggat tgggaatttc caccacctct tcagccagaa
    61441 ctgagcccat aatggcaaat tgcaccaaca ttgtgctgtc acaatcgacg ttggccccta
    61501 aatcgaggac caccgttttg cccttttgct gatgtggtaa taccgtcacc agcgccggac
    61561 gctcaatccc ctccaggggc ttgagtaata attttgccag ccccatcagt gccccggtat
    61621 taccggcact gacacaggct tgcgctcgac cttctttcac cagctccagg gccatacgca
    61681 ttgaactccc acggctggcg cggatagctt gcgaaggccg ggcatcactg gcgataactg
    61741 actgcgcagg aataatctgc agacgcgaac gttgttcaaa gtcagcttta gcaagtaatg
    61801 gcgtgatggc gtcgggattg ccgactaaaa gaagagtgag ttgcgaatta gaattcagtg
    61861 cctgcaatgc tgcaggcact gtcacggaag ggccaaaatc ccctcccatg acatctaacg
    61921 ccagggttag acgtgtcaag gtatcgtcgc cgcccggttt ggtctttccc cagttgcctg
    61981 gggaaaatcc tcactaagct tcatcacgca gacgcgtgat tacttagcga tgaccttgcg
    62041 gccgcggtag taaccgtcgg cagtgatgtg gtgacgcagg tgtttttcac cagaagtttt
    62101 gtctacagac aggctggtga ctgcggtcag cgcgtcatgg gaacgacgca tgccacgttt
    62161 ggaacgggtt ggtttattct gttgtacggc catggacctt actcctcaat tacggtttat
    62221 ccccgatcca acggggagca ccaattactt acgctttaag ctggctaata cggcaaatgg
    62281 gtttggcttt tgcacttctt caggcagttc accaaagacc atgtccgctt cggacacttc
    62341 acagtgttca gaatcatgca ccggaactac cggcaaggcg aggatgattt catcttcaac
    62401 cattgcaagc agatcgattt caccgaattc gttaacctca atcggttcat acgcttccgg
    62461 cagtgcttca gcctgttcgt ctgaacgcac aggactaaaa caatacgttg tgtagacctg
    62521 atgagtaaac ggcttcccgc aacgctgaca ctcgagcgtt accgtcacct tcgcatcgcc
    62581 gtttaacact gcgagacgtt ggttatcgat agcgaacgac atggagcatt ccacatcact
    62641 gtccacactg actacggatt cggcgacgcg ctcaacctga tcaggggtat agataccctg
    62701 gtaatcaagg cgtttttgag ccgtacgaac cggatcgaga gtcaggggta attttacctt
    62761 ttgcataggg cgcgcatatt aactttgtaa cgtcatagag tcaaagaaaa aggcagcctg
    62821 tggttgcctt ttgccaataa ttcgcacaca ttgcgggtta ctgctttatt ttcgttcagt
    62881 gctgataacg aacatcaacc tgcaaaacga agcgcatagt ttaaaatggc tatcatcaat
    62941 acgctatatc tgatggaaaa aaatatgcct aaacttattt tagcctccac ctcgccatgg
    63001 cgtcgcgctc ttctggaaaa actgcaaatc tcttttgaat gtgcagcacc tgaggtcgac
    63061 gaaaccccac gcagcgacga atcaccgcga cagttggtgc ttcgactggc acaagaaaaa
    63121 tcgcaatctc tggcgtcacg ttatccggat catttaatta ttggttcaga ccaggtatgt
    63181 gttcttgatg gcgaaatcac cggtaaaccg ttaacggaag aaaatgcccg tctgcaatta
    63241 cgcaaagcca gcggcaatat cgtcaccttc tatactggac tggcgttgtt taattcggcg
    63301 aatgggcatc tacaaacaga agtggagcct tttgacgtcc atttccgtca cctaagcgag
    63361 gcggagattg ataattacgt gcgtaaggag catcccctgc actgcgcggg tagctttaag
    63421 agtgaaggat ttggcattac gctgtttgag cgtttagagg gacgtgaccc taacacgctg
    63481 gttggtttac cgctaatcgc gctgtgtcag atgttacgtc gggaagggaa aaacccgctg
    63541 atgggatagt tgtttgcgcc ggatgcggtt acgtcgcatc cggcaatcgg tgcaggcggc
    63601 ctgatgcggc gtaaacgcct tatcaggcca accgcccatt atcaggctta tatgcttgta
    63661 ttagcgcgcg ttacgcagct tttgcaaaca acgcttcaaa ccttcatcca tcggcgcttc
    63721 gatacgcatc acctcaccgg tccccggatg ggtaaacttc aacgctgcgg cgtgcaggaa
    63781 cagacgattt aatcccgtgc ctgcttcagt gagctgtctg tcaaattcac ggtcaccgta
    63841 gcgatcgtca aaggcaatcg gatgacccgc atactgtgta tgcacacgga tctgatgagt
    63901 acgtccggtt accgggctac aacgcaccag ggtggcaaat gcatagcgtt cttccacttt
    63961 aaagcgtgtt tccgacggtt tgccttcctg actgacacgc acgatacgtt cgccgctttg
    64021 cagaatattt ttcaataacg gcgcttgaac gctcttcaca tgcgactgcc actgaccgcg
    64081 caccagcgcc aggtaatctt tttgcattcc tttttcacgt aattgctcat gcagagaacg
    64141 caacgccgag cgttttttcg ctaccagcaa cacacctgaa gtgtcccggt cgagacgatg
    64201 aaccagttca aggaaccgcg cttccgggcg caacgcccgc aaaccttcga taacgccgaa
    64261 gcttaaacca ctgccgccat gtaccgccgt accggaaggt ttattcagca ccaggatatg
    64321 atcatcttca tataagatga cgtccgccag cgccgccacc ttttgcagat gtggcgaaac
    64381 cgcctcttct tcccgctcag caacgcgaac cggtgggata cgcacctcat cacccgcttc
    64441 gagtttatat tcaggcttaa tacgtttttt gttcacccgc acttcacctt tacgcaaaat
    64501 acggtaaatc atactttttg gtacgccttt caattgggta cgcaaaaagt tatcgatacg
    64561 ttgccccgct tcgtcagcgg tgatagcaac aatttttacg gatggagtct ctgttttcat
    64621 ggtgggcgat tctaattagc caacaggatt cgcgccactc atttttctat gcttattttt
    64681 agttttgcac cttattactt cactacgtga tcactttatt gatggttatt aaaccaatca
    64741 ccagcaagaa gtgaaaaaac tgtgagtaag cgggtgataa atggtaaaag tcatcttgct
    64801 ataacaaggc ttgcagtgga ataatgaggc cgtttccgtg tccatccttg ttaaaacaag
    64861 aaattttacg gaataaccca ttttgcccga ccgatcatcc acgcagcaat ggcgtaagac
    64921 gtattgatct ttcaggcagt tagcgggctg cgggttgcag tccttaccgg tagatggaaa
    64981 tatttctgga gagtaatacc cagtctgttt ctttgataat tgcgctgttt ttccgcatga
    65041 aaaacgggca accgacactc tgcgcctctt tgagctgacg ataaccgtga ggttggcgac
    65101 gcgactagac acgaggccat cggttcacac ccggaaaggc gttactttgc ccgcagctta
    65161 gtcgtcaatg taagaataat gagtaagtta cgatgaaaag aatgttaatc aacgcaactc
    65221 agcaggaaga gttgcgcgtt gcccttgtag atgggcagcg tctgtatgac ctggatatcg
    65281 aaagtccagg gcacgagcag aaaaaggcaa acatctacaa aggtaaaatc acccgcattg
    65341 aaccgagtct ggaagctgct tttgttgatt acggcgctga acgtcacggt ttcctcccac
    65401 taaaagaaat tgcccgcgaa tatttccctg ctaactacag tgctcatggt cgtcccaaca
    65461 ttaaagatgt gttgcgtgaa ggtcaggaag tcattgttca gatcgataaa gaagagcgcg
    65521 gcaacaaagg cgcggcatta accaccttta tcagtctggc gggtagctat ctggttctga
    65581 tgccaaataa cccgcgcgca ggtggcattt ctcgccgtat cgaaggcgat gaccgtaccg
    65641 aattaaaaga agcactggca agccttgaac tgccggaagg catggggctt atcgtgcgca
    65701 ccgctggcgt aggcaaatct gctgaggcgc tgcaatggga tttaagcttc cgcctgaaac
    65761 actgggaagc catcaaaaaa gccgctgaaa gccgcccggc tccgttcctg attcatcagg
    65821 agagcaacgt aatcgttcgc gccttccgcg attatttacg tcaggatatc ggcgaaatcc
    65881 ttatcgataa cccgaaagtc ctcgaactgg cacgtcagca tatcgctgca ttaggtcgcc
    65941 cggatttcag cagcaaaatc aaactgtaca ccggcgagat cccgctgttc agccactacc
    66001 agatcgagtc gcagatcgag tccgccttcc agcgtgaagt ccgtctgcca tccggtggtt
    66061 ccattgttat cgacagcacc gaagcgttaa cagccatcga catcaactcc gcacgcgcga
    66121 cccgcggcgg cgatatcgaa gaaactgcgt ttaacaccaa cctcgaagcc gccgatgaga
    66181 ttgctcgtca gctgcgcctg cgtgacctcg gcggcctgat tgttatcgac ttcatcgaca
    66241 tgacgccagt acgccaccag cgtgcggtag aaaaccgtct gcgtgaagcg gtgcgtcagg
    66301 accgtgcgcg tattcaaatc agccatattt ctcgctttgg cctgctggaa atgtcccgcc
    66361 agcgcctgag cccatcactg ggtgaatcca gccatcacgt ctgcccgcgc tgctccggta
    66421 ccggtaccgt gcgtgacaac gaatcgctgt cgctctctat tctgcgtttg atcgaagaag
    66481 aagcgctgaa agagaacacc caggaagttc acgccattgt tcctgtgcca atcgcttctt
    66541 atctgctgaa tgaaaaacgt tctgcggtga atgcgattga aacgcgtcag gacggtgttc
    66601 gctgcgtgat tgtgccaaac gatcagatgg aaaccccgca ctaccacgtg ttgcgcgtgc
    66661 gcaaagggga agaaacgccg accttaagct acatgctgcc gaagctgcat gaagaagcga
    66721 tggcgctgcc gtctgaagaa gagttcgctg aacgtaagcg tcctgaacaa cctgcactgg
    66781 caacctttgc catgccggat gtaccgccag cgccaacccc agctgaacct gccgcgactg
    66841 tcgtagcccc agcaccgaaa gctgcaacgg caacaccagc agctcctgca caacctgggc
    66901 tgttaagccg cttcttcggc gcactgaaag cgctgttcag cggtggtgaa gaagccaaac
    66961 cgaccgagca accaacaccg aaagcagaag cgaaaccgga acgtcaacag gatcgtcgca
    67021 aacctcgtca gagcaaccgc cgtgaccgta atgagcgccg cgacacccgt agtgaacgta
    67081 ctgaaggcag cgataatcgc gaagaaaacc gtcgtaatcg tcgccaggca cagcagcaaa
    67141 ctgccgagac gcgtgagagc cgtcagcagg ctgaggtaac ggaaaaagca cgtaccaccg
    67201 acgagcagca agtgccgcgt cgtgaacgta gccgccgccg taacgatgat aaacgtcagg
    67261 cgcaacaaga agcgaaggcg ctgaatgttg aagagcaatc tgttcaggaa accgaacagg
    67321 aagaacgtgt acgtccggtt cagccgcgtc gtaaacagcg tcagctcaat cagaaagtgc
    67381 gttacgagca aagcgtagcc gaagaagcgg tagtcgcacc ggtggttgaa gaaactgccg
    67441 ctgccgaacc aattgttcag gaagctccag ctccacgcac agaactggtg aaagtcccgc
    67501 tgccagtcgt agcgcaaact gcaccagaac agcaagaaga gaacaatgct gataaccgtg
    67561 acaacggtgg catgccgcgt cgttctcgcc gctcgcctcg tcacctgcgc gtgagtggtc
    67621 agcgtcgtcg tcgctatcgt gacgagcgtt atccaaccca gtcgccaatg ccgttgaccg
    67681 tagcgtgcgc gtctccggaa ctggcctctg gcaaagtctg gatccgctat ccaattgtac
    67741 gtccgcaaga tgtacaagtt gaagagcagc gcgaacagga agaagtacaa gtgcagccga
    67801 tggtgactga ggtccctgtc gccgccgctg tcgaacctgt tgttagcgcg ccagttgttg
    67861 aagaaatggc tgaagtcgta gaagcccccg taccggttgc cgaaccgcaa ccggaagtgg
    67921 ttgaaacgac gcatcctgaa gtgattgccg ccgcggtaac tgaacagccg caggtgatta
    67981 ccgagtctga tgttgccgta gcccaggaag tcgcagaaca cgcagaaccg atggttgaac
    68041 cgcaggaaga gacggcagat gcagatattg aagaagttgc cgaaactgct gaggttgtgg
    68101 ttgctgaacc tgaagttgtt gctcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag
    68161 aagttgaaac ggtggccgcg gttgaaccag agatcaccgt tgagcataac cacgctaccg
    68221 cgccaatgac gcgcgctccg gcaccggaat atgttccgga ggcaccgcgt cacagtgact
    68281 ggcagcgccc tacttttgcc ttcgaaggta aaggtgccgc aggtggtcat acggcaacgc
    68341 atcatgcctc tgccgctcct gcgcgtccgc aacctgttga gtaataatta gctcaaagta
    68401 atcaagccct ggtaactgcc agggcttttt tatttcatgt ttgaatcaac atattaaaat
    68461 tcatcaaatg gcatccttgc taaccaacaa tgcaaaatag gcaaaaaaaa tgcccgcccc
    68521 ggcgatccca gagcgggcag acaaaacata cccagtttca tgatatgttt taaagcgagc
    68581 ttatttgttg agctggaaga gcgacaatcc ctgcatatcg gtaaatgctt tatacgatgc
    68641 ctgcaatgcc gtttgctgca tgatgtaaga tgaaatagtt gcattccagt ccacatcaac
    68701 cagatcgctc atctgctgcg tttgccctaa agcgcgatcg ctacctaagg aatccagcga
    68761 ctccagtttg ttcagctgcg tgcctaattc cgcgcgaacg gtaaggacat tattcagcga
    68821 gtttttcagt ccacggttgg ttttatccaa tgccgcagcg gcgatttctt tatccgcttc
    68881 gctatccgcg accggcgttt tcagggctgc gatggcgcta tccagcatgg caaaaagatt
    68941 ggtttcagaa gcgctaccgt ctggttccgc taccgcgtta ctggtaatac tgtcgaaaat
    69001 tttgtcgccc gtatgtccta tcaccatcga acgcgaagca tcgacctgtt gcttaatact
    69061 ttctgttccg ccaacataat caccgttagc ctcactaaac ggcgcagtct ctgttttata
    69121 tgtagcgccc attaccgtca gtggtgttcg ccagattcag caactggtca cgaagcccct
    69181 gaatatccgt agccagcgag gcccggtcat cgtcactcaa ggtgccattg ctggcgtaga
    69241 caattttttc ctgagcattc tggatagcag tggtgacctg gctaagtaca ctctcttcca
    69301 gtgacacttt ttgagtggcg aaagtacgtg ccagcgtgta ctggctgttt tgcgcctgtg
    69361 cctgggagag aacaacagct tgtgatgcag caatgggatc gtctgaaggg ttaacgactc
    69421 gcttacccgt cgacatctgc tcgccgtact tcatccattc tgcctgagaa ttggtgatgc
    69481 cgcgcatgtt ttgctggtac atcatctggg tactgaaacg cattttatct ccccttagcg
    69541 aatgttaatc agcgcatcaa aaatcgcgtt tgccgtctgc agaacctgcg cattcgccag
    69601 gtaatactgc tgaaaacgtt gcagatttcc gtactcctca tcgagattga caccggaaat
    69661 cgactgctgc tgattggaaa gctgcgtcac cacattacct tgcgtggtgc tactggtttt
    69721 caacgtcgcg gttttattac cgatatcact cactaacgag gcataagcgt cgttaaagga
    69781 tttcgcaccg cccaccgttt tactgttgct ttgcagatcc agcagggcct gaccgttgcg
    69841 gttgtcgcta tcacccgcat cttcttcgct cgccatcgcg attttcgctt cgtcggtgat
    69901 taatacatcc atgttgacga tggcgtcact tactggtttc agcgtgaagc tgtcgttaac
    69961 ggcaggcgtt cctgtaaacg tcaactccag accatcaaat gccactttac cgttggcatc
    70021 tggtgtcgcc gtaaaagtgg tattgctggc aaggcgggtg acctgccact gattattatc
    70081 gaacgagatt ttgtaatctg tcgccagtac cacggaggca tcagttaccg tggcaccgat
    70141 cgcaacgtca cctttgtttt tcgtgttttg cagaaccgcg ggcttaccga tagtaaagaa
    70201 atcttcacca gcatcgccgt tagcatcaaa tccggctttg tgttggctgt tgaaagcctc
    70261 ggcaaatgcc agcgccagtt gtccaagcgt attacgcgtc tggtccagat cctgagaacg
    70321 gaatgtcaga atgccgccca gcgacccggt attcagtaat ttctccggga tctcaatatt
    70381 gcctgccgtc ccatcaacgt aagcgacagt cgtacgagaa gggtcggcgc tggaaggaac
    70441 tgccgccagt tgccgcgccg tacttccctg aaccagtgag taaccattgg catcgtgatg
    70501 ttataagtac cgccatcctg aacgctgact tctacgccaa caatctggtt taattcgctc
    70561 accagttgat cgcgttgatc cagcagattg ttaggtgacg cccctgcccc cacgcctgtc
    70621 aggcgcgaga tttgatcgtt caggctggca atttgtttag cgtaattgtt gatctgatca
    70681 acgctggcac ctatcgcgat attgacctgt ttgtcctggt cgcgcagata ttgatcggtg
    70741 gttttaaact gattcaccaa cccttctgat ttcccaatca gcgcctggcg cgccgccggg
    70801 tcttccgcgt tactcaccag cgtttgcagg ctggtgaaga aatcctgcat ctgtgttgcc
    70861 agcgaagagg tactggtgga gagcatattg tcgattttcg acatctgctc atagcgggcg
    70921 gtcaggccgc tactttgcgt ctgcgcagca cgtaactggt tggtaataaa cgtatcatac
    70981 tcacgctgca caccagaaac gtagacgcca ttgccaaccc agccgccagc gcccaacgtg
    71041 ctattggcct gcgccataat agtggtttgg cgggtatatc cggcaacgtt atagctggag
    71101 atattattac ttgccgtatt taacgccgcc tgggccgcgt tcagtccgct catggcgtta
    71161 ttgatcaagc tggacatgga ggttccttat aagccttcaa ttacagagta ttatcggcag
    71221 cgacccgccg gacttgagtt attcagaaca gattatcaat gttcatgctg taggttttgc
    71281 tcaccttgtc gcttatcgat ttcatctgct ggatcatgtt ggtgagttta cgggcatagt
    71341 gaggatcggt agcatagccc gcgtcctgta ggacctgcgc cccctgttcc gcactcgcgg
    71401 cggtcgtcac ggcggcgtag cgcgggttac gtgttaacag cccaacgtaa tccgacaatg
    71461 cttccagata cgagctgtag acccgaaact tcgcttttac tttcttcgct tcgccgtttt
    71521 catattcagt cgtggtgatt tcagtgactt gccctttcca gttgccagag gctttgacac
    71581 caaacaggtt atagcttggc tcgccgtttt cgcggcggat ttgccgttgc ccccaaccag
    71641 attcaagcgc agcctgagcg aggatcaaat gatgtggcac accgctttgc tggcttgcca
    71701 gttgggcggg cagcgagagt tgcgcgagga atgctctact gtcacccggc agcgaatcat
    71761 cgtagttacg tggtacggcc ttttgcacca gctgcgaaag cgtctggttt tgataacgca
    71821 ccacggtttc gagcgggaat ttcatcggtg ctgctggcgt ggactcctct ggcaatggtt
    71881 gttctggcgt catctgttta accatcatct ctgcaagccc cagacctttg cccgccgtca
    71941 tctgttgggc aatttgctgg tcatacatac tggtatacag acgagtgtgc tcgctgctga
    72001 acaggccatc ttttggtaaa gcgtcgcgca tgcttttcaa catcatctgc acgaacatcc
    72061 cttccacctg acgggccacc ggacggatat ttgccgccgg atcttcgctc gctttcgcct
    72121 ttagttcgtt gagtgattgc gcatcccagg ccgcacttgc cagtagtttg ctgtcgctga
    72181 tcatcagatg atttccagtt ttgcccgcag acatcccgca ctttgcattg attgcaaaat
    72241 agacatcaga tccatcggcg tagcgcccag cgcattgagc gcacgcacca cgttattgag
    72301 gctggcgctg gaacgtacgc tttgcagcga accgccgctc tggcgtaaat cgatctgcgt
    72361 ttgtggagta acaacagtct gtccaccacc aaacggtgta tctggctggc tgacattggc
    72421 ctgacgatta actgttactg agagatttcc ctgcgctacc gcgcagctgt cgagggtcac
    72481 ttcgcgattc atcaccaccg aaccggtgcg cgagttaatc actactttag cgtcctgcgg
    72541 ggtgacatta acctgcatat tctggatatc ggcaaggaaa cggacctggg aactgttgcc
    72601 acttggtacg cgcacctgaa tagtccgcgc atctaacgcg gtggcgctgc catatccacg
    72661 cacgcggttg atggtgtcag cgatttgctg cgccatgctg aaatcttcgt cgttaagttg
    72721 caaattaagg gtattcccga cgccaaactg gctgggcaat tcacgttcaa taaccgcacc
    72781 attggtgatc cgtccaccgt tcagttggtt aacctgaaca ctgctaccgc ctgcggaggc
    72841 tcctgcgccg ccaaccagaa tattgccctg cgccagcgca tacacctgac tgtcaacgcc
    72901 cttaagcgga gtcatcaaca acgtacctcc acgcaagctt ttggcattac ccatggaaga
    72961 aaccaccaca tcgatggttt gcccctgacg tccaaacgga ggaagtgacg ctgtcaccat
    73021 taccgcagcg acgtttttta gctgcatatt ggtgcccgtc ggaacggtaa ttcccagctg
    73081 tgagagcatg ttattaagcg tttgtgtggt aaacggcgtc tgggttgtct ggtcaccagt
    73141 gccatccagc cccactacca agccatagcc aatcagtgag ttttgcctta ccccctgaac
    73201 actggtgaga tcgcgaatac gctcagcctg agccgccgtc gtaaccagta gaagaattaa
    73261 tgcagagaga aatttaatca ccacagcctc acttacattg gcgacaggtt aaggaagaaa
    73321 cgctgcaacc agcccatatt ttgcgcttcg ttaatgtagc cattgcctac gtattcaata
    73381 cgcgcatccg ccacctgagt agacggtacg gtattgctgc cgctgatagt gcgtggatta
    73441 accacgcccg agaagcgaat aaattcggta ccctgattaa tggcaatctg tttttcaccc
    73501 accacatgca ggttgccgtt gaccagtacc tggtcaaccg tcaccgtcaa cgtgccgcta
    73561 aaggtattgc tggcattggc cccgcccttt ccgttgaacg tgttaccacc ggaggcttcg
    73621 acatcggcac gagcgttacc aaacagcccc tgcaaatagc gcggcacagt atcaaagcca
    73681 aaattagttt taccgtcacg gctggcattc gcagaggagc ttttgctggc gctgacgttc
    73741 tcctgcaaca cgatggtcag cgtatcgcca atattgcgtg gtcgacgatc ttcaaacagc
    73801 ggttgatagc catagttaat cggctgagca gactggaaaa tagaaccgtt ggcgacgggc
    73861 gtcggaccgg gaaccggttg tgcactggtc gccccctgca ccagcggcgt ggagggtatc
    73921 caggcgcagc cggttagtga aagcaccaac aagctggaaa tggcataagt atgcgcagcg
    73981 tttttttgca tggctatcat cttcaaaaat cagtaaaccg gtgaacctgc caccggttat
    74041 gccttagagt tgcgtcagtt tttgcagcat ctgatcggtg gtggacaccg ctttactgtt
    74101 gatttcgtaa gcgcgttgca cctgaatcat attgaccagt tcttccgcca cgttgacgtt
    74161 agacgtttca acataccctt gatacagcag tcccgcgccg ttcaggcccg gcgtgctttc
    74221 gttcggtgca ccggaggatt gcgtttcggt gtagaggttt tcgccaatgc tctccagtcc
    74281 ggtatcattc ataaaggtgg tgagattgag ctgcccaacc tgaaccggag ctgcctggcc
    74341 ttgttgggtt acgctgacca cgccatcacg accgatggtg atacttaacg cattcgccgg
    74401 aatggtgatc gctggctgca cctgaaaacc accagccgtc accagctgcc cgttctgatc
    74461 cacctggaaa gagccgtcac ggatataggc tgatgagcca tctggcaaca ttacctggaa
    74521 aaagccctgc cctttaatcg cgacatcttt gctgttattg gtctgcgaca gattgccctg
    74581 gctgtgtaag cgttcagtgg cgaccgggcg tacccccgtg ccgatttgta atccggaggg
    74641 taaggtggtt tgttcggaag actgtgcccc cggctggcga atggtttgat aaagcagatc
    74701 ttcaaacacc gcgcgctgac gcttaaaacc gttagtactg acgtttgcca ggttgttggc
    74761 aatgacgtcc atattggttt gctgggcgtc aaggcccgtt ttggcgatcc ataatgaact
    74821 gatcatataa tgtcctgtag tggattgctg acaagcagag cagaagcacg cgacagtgtg
    74881 aatttcccct caccctaacc ctctccccaa aggggcgagg gaatagtccg cgcgctttta
    74941 ttgcaatgtc atcaatcgtt caattaactc atcgacagca gttggttggc acggcctgcg
    75001 ttatcatcga cgctgctgat caccttcatc tgcatttcaa aacgccgcgc gctggcaatc
    75061 atgtcgctca ttgccgcaac ggcattgacg ttactgcctt ccagaacccc cgacatcaca
    75121 cgcaaggttg gatctgcctg cagtaccggc cctcgcgtgg cctgggtttc tgcgcttaaa
    75181 cgaaaaatgc cgtcgtcacc gcgctgcact tcgctgcccg tggctttcac cagtttcaga
    75241 cgccctactg gcgcaactgt atttgccgga tcgcccggat tgagcgccga gattgtgcca
    75301 tcggcagcaa tcgtgatttc cgccccttcc ggcacagcaa ttggccccgc ctcgcctatc
    75361 accggatgcc cctgaattgt cagttgcccg gtgggatcaa cctgaatgct gccattacgc
    75421 gtataccctt cgctgccgtc agcggtctgc acggccagcc atcctgctgc aacgcaacgt
    75481 ccagcgggcg agaggtgtaa tccatcttgc cgggcgtcat gtctgcaccc ggtgttgacg
    75541 ccgttaccag cgtgcgcgtg ggcagtgaaa gcccttccac cggcactgcg cgcaaggcgt
    75601 ttaactgcgc gcgaaaaccg ggcgtagagg cattggccag attactggcg gttaccgcct
    75661 gttgattcag cgtctggctg gctgctccca tcgcagtata aattgcgtga tccattgagc
    75721 tatcccgtca gcgattagcg taagttaacc cgcgtgttga ggatctggtc ctgggttttg
    75781 atggtctggg cgttagactt atagttacgc tgggcaacga tcatattgac cagttcttta
    75841 ctgagatcga cgttggacgc ttccagcgca ccgttggtca gagtgccaaa gtttcccgtc
    75901 ccggctgtcc ccaacagcgc cacgccagaa gattgcgtcg cagaccagac gttgtcgcct
    75961 tcggatgcca gaccttcgtt gttggcaaag ttcgccagta caatctgccc cagcagttgg
    76021 gtttgttcgt tggaattgtt gccgacaacc gtaccgtcat cattgatttg ataactcacc
    76081 agatcgcccg gtttgtagcc attctgggtg gttgccacaa tgttgttagc gccggttgcg
    76141 atattattcg ccatcgcacc atccactaat gtgccattag cattaaattc cagtgttgtc
    76201 gctgtcttcg caatgctgtt tggatcactg ctatcctggg tgtagacctg ccagttatta
    76261 tccccggtct tcacaaaata gacgctcatg tcatgagcat taccctgact gtcgaaaaca
    76321 gtcaccgaac cttttttgtt atagctatcc gcattgctgg cgctgaatgg cgtaacagta
    76381 ggaagcggat cactggaatt caggttgatc tgcatcgacg ccgtggtggt agttttcgct
    76441 gccatcaggg tattcgggat cgaaatattg gtcggattcg ccccttgctg aatagtcggc
    76501 ggcgtaccgg ttaccgggta acccgtcagc tgtaaacctt gcgtattcaa caggttacgg
    76561 ttttcatcca gcttaaattg tccgttacgg ctgtagaaca ccgaaccgtt gctgtctacc
    76621 agacggaaaa aaccgttctg gctgatagca acgtccagac ctcgcccggt gttggtggtc
    76681 gtgccatcgg taaagtcctg agtgataccg gcaactttta cccccagtcc cactttcgaa
    76741 ccggcaaaca tatcggcaaa agaggccgtg cctgatttaa agccgtaggt ggcggagttg
    76801 gcgatattgt tgccaataac atcgaggttg gtggcggcag cgtttaatcc gctaaccgct
    76861 tgagaaaagg ccatgactga ctcctgaaat gtgaaggctt aaattatctg ccgtacttcg
    76921 tcgagggtgg tggtgccgta agtgccgaga tccagcgtat taccgctgtt gccgcggatc
    76981 acaccctgca ccagagcaaa ctgcagcggc tgggcaacca gttgtgtacc accgttactg
    77041 gcgctaatcg ctacattgta agaaccgttc ggcgcagtgc tgccatcagt caacgaaccg
    77101 tcccaggtga aactgtgaac tccggcggtc agttcaccaa tatcaatggt gcgcacaacc
    77161 gcgccatttt tatcggtgat ggtggccgta actttgtctg ccgcctgttg cagctcaaca
    77221 ccaaacggcg tggtcgtggt cacagcccct tcttcactgc cggttcccgc aagaacagtt
    77281 gtgccgggga tcatcacgcc gtgaccgatc aggttactgg cctgtaacga ctggctgtta
    77341 tcaatctgtc cggaaataga tccgagcgtg gtattgagtt tttcaatccc gctgaccgtg
    77401 ctgatttgtg ccaattgcga cgtcagctcg ttgttttcca ttggattggt cgggtcctgg
    77461 tttttcagct gcgccaccag caaagtcaga aaactgcttt gtaaatctgc ggcgttgctg
    77521 cccgtgagcg aactactgct ggtggtactg acgccggtat ttgtcggatc ggtggtggtt
    77581 accgcaatgg acatagcttt ctcctttatt gaccgagcgt aagggttttc agcatcatgc
    77641 ttttcaccgt gttgagcact tcaacattgg cctgatagct gcgtgacgcc gacatggtgt
    77701 taaccatctc cccgacgaca tcaacgttcg gcatttttat gtagcccttt gcatctgcca
    77761 gcggattacc tggttcataa accagtttgt ccggggcctg actttcttaa catcggcaac
    77821 ctttacgccg cctgtcgcag cacctggtgc agcgttaacc tggaatacca cctgttttgc
    77881 ccgatatggc tgtccatcag gaccggtcac gctatcagca tttgccagat tactggccgc
    77941 cacgttcagg cgctgggact gggcagttaa cgccgacccg gcgatatcaa aaatattcag
    78001 cagtgccatc cgttaatttc cgctctgtaa aacgttcatc atgcctttga tttgcccgct
    78061 caacgcgcta aggctcatct ggtattgcag gctgttatcg gcaaactggg tgcgttcgcg
    78121 atccatatcg acggtattac cgtcaagcga aggctggtcc ggaatacggt attgcagttc
    78181 tgcggtagga ggcgtcagcg cctgcgccgg gatgtgctgc gttgaggtca tcgtcagtgc
    78241 aaccacactg gttgcatccc gtccacgttg catgactttt ttaagttcac tggcaaaatc
    78301 gatatcgcgc gcctgataac caggggtatc ggcattggcg atgtttgctg ccagcacttc
    78361 ctgacgctgg gcgcgcagat tgagcgcctc ttgttgaaaa cgtaaggcgg cgtcgagctt
    78421 atcgagcata tctcctccgc aggtatcaaa attctgccat cagcttaaat gcctttacgc
    78481 gcgcgttatc ggcggaataa acgcaaaatg ggtcgctatt tatgccgttg atggtcattg
    78541 cggacaggta caattcacgt tgtagaaatg gctgggggcg aaaatgctga caataaaacg
    78601 tagcgtggcg atcatcgcga tactgttcag tccgttaagt acggcgagca atctcacatc
    78661 gcaattgcac aactttttta gcgcccaact cgcgggggta agtgatgagg ttcgtgtttc
    78721 tattcgtaca gcgcccaatc tactaccgcc atgcgagcag ccattgcttt cgatgagcaa
    78781 taattcccgc ctgtggggca atgtgaatgt gttggcacgc tgcggtaacg acaaacgata
    78841 tttacaggtt aatgtgcagg ccacaggaaa ttatgtggtt gccgcgatgc ctattgtgcg
    78901 gggaggaaag ctggaagctg gcaatgtcaa actgaaacgt ggacgcctgg ataccctgcc
    78961 accgcgtacg gtgctggata tcaatcaact tgttgatgcc gttagcctgc gcgatctatc
    79021 acccgatcaa cctatccagt taacccagtt tcgccaggca tggcgggtaa aagcgggaca
    79081 acgcgtcaat gtgatcgcca gcggtgatgg gtttagcgcc aacgcagaag gtcaggcgct
    79141 gaacaatgca gccgtcgcac agaatgcgcg ggtgcgcatg gtatcggaac aggtagtcag
    79201 cggcgttgtt gatgcagatg ggaatattct tataaacctg taagctgtta aagattaccc
    79261 gtcccttgcc gataaataag caacacatga taaaagcgcc ctcaatgagg aataaaccat
    79321 gagtattgat cgcacttcgc ctctgaagcc tgtaagcacc gttcaaccgc gcgaaaccac
    79381 cgacgcgccg gtaacgaaca gccgggcggc aaaaacaacc gcctccacca gcaccagtgt
    79441 gacgttaagc gacgcgcaag caaaactgat gcaacccggc agcagtgata tcaatcttga
    79501 acgtgtcgaa gcgttaaaac tggcgattcg taacggtgaa ctaaaaatgg acaccggcaa
    79561 aattgccgat gcgctgatca acgaagcgca gcaagacttg cagagtaact gatcgtatga
    79621 cacgtcttgc agagatcctc gaccagatgt ccgctgtgct taacgatctc aaaacggtaa
    79681 tggatcaaga gcagcaacat ctctctatgg ggcagatcaa cggcagccag ttgcaatgga
    79741 ttacagaaca aaaaagctca ctgctggcga cgctggatta cctcgaacag ttacgcaaga
    79801 aagaacccaa tacagcaaat agcgttgata ttagtcaacg ctggcaggaa attactgtga
    79861 aaacgcagca actacgccaa atgaatcaac ataacggctg gttactggaa ggacagattg
    79921 agcgcaatca acaggcgctg gaaatgttga aaccgcatca ggaaccgacg ctatatgggg
    79981 cgaacggtca gacctcaaca ccccatcgcg gcagtaaaaa gatttcgatc tgaatattat
    80041 ctccggcctg cactgcaggc cggaatgcat tgttacaccg tccggcgggc aaattcttta
    80101 actttgaagc ccagtaccgc cagtgcagcg aagtacgcgg caatccccgc cagcacgacc
    80161 gccattaaac gcagtaagcg ccagggcatg gtacccaatg accactccgg catgatatgt
    80221 aacataccta aaagcacgcc agacatcacc agtaccgcca ccaccagacg caacagaaac
    80281 gccatccagc cgggttgcgg agtaaagatt ttctgcttac gcaactgcca gtaaagcagc
    80341 gaagcattca gacacgccgc cagaccaata gaaagtgaca gcccggcatg tttcaacgga
    80401 ccaataaacg ccaggttcat caattgcgtt aaaatcagcg taacgatggc aattttcact
    80461 ggcgttttaa tgtcctggcg ggaataaaag ccaggagcca acactttcac tacaatcagg
    80521 ccaatcaaac ccaccgagta ggcaattaac gcccgctggg tcatcagcgc atcaaacgcg
    80581 gtaaatttac cgtactggaa cagcgaaacg gttaacggac ctgaaagaat gcccaacgca
    80641 accgcactcg gcagtgccaa caggaaacaa agacgcaacc cccagtccat caaacggttg
    80701 tattcatcat gattgccact ggcaaaactt ttcgacagcg acggcagcaa aatggtgcca
    80761 agcgccacgc ccagcacacc ggagggaaac tccattaagc ggtcggcgta atacatccaa
    80821 gacaccgaac cggaagcaag aaacgaggca aaaatggtgt tgatgattaa ggagatctgg
    80881 ctcacagaga cgccaaggat cgccggtccc atctgtttca ccacgcgcat tgctccagca
    80941 tcgtggaagt taatgcgcgg caggaccagc atgccgatct tcttcaggtg cggtagctga
    81001 tacaccagct gcagaatgcc gccgaccgta acagcccacg ccagcgccag caccggtggg
    81061 ttaaagtacg gtgcggcaaa cagcgcgaaa ccaatcatgc tgatgttaag cagtgttggc
    81121 gcaaacgccg gaatcgagaa gcggttccac gtattcagaa tcgctcccac cagcgacgcc
    81181 agggagatca gcaagatata gggaaaggta atctttagta gctggctggt cagggcaaat
    81241 ttgtcagctg tgtcagcgaa gcctggcgcg gtcaccatga tcacccacgg tgcggcgagc
    81301 atgccagcga ccgtcacaac cgccagcgca agtgtcagca ggccagaaac ataagagaca
    81361 aagacccgcg tggcgtcttc accctgctta cttttatatt ccgccagaat cggtacaaat
    81421 gcctgggaaa atgccccttc ggcaaagata cggcgtaaca agttaggaag tttaaaagcg
    81481 acgaaaaagg cgtcggttgc catccctgcg ccaaagattc tggcgacaat tgcgtctcgt
    81541 gcgaagccaa gcacacgcga aaacatggtc atcgagctga cggcggccag cgattttaat
    81601 aaattcatcg gtgttctaat ccggacccac gggcaaaacg cctgcaaggc aggcgctgaa
    81661 agaggcgaat agtctaccta gattatggtg aattgctacc gccagatgtt acagggttta
    81721 ttcactcatc gcatcgcgcc agatcttgtc aacgatacgt tgcgccagca ctgcctattc
    81781 gccggcggtt tgcggaactg tctgatttta cacgcattca atgaagtgcc gcgcacagcc
    81841 gacaaatcca cgttgctcaa gagtgctctg ccaaccagga atcggtttat tcactacacc
    81901 ctgcccgcgc tcctcacgcc attcgcgcat atccgtaatg tcgatgagcg caccgtcagt
    81961 cacggcctgc acggtttcac gctgacttcc ggcacggcga tgcatacaag tggtgatctg
    82021 caaaggaccg gccgaaaaat ggtgctcggc aaacagcatt tcgccagcgt cgttagtcag
    82081 tagcgtaccg ccatccagag aggctttgcc gcccgacaac cacagcgcgg tatccaccac
    82141 atgcagataa tcatccagca acgtgaaata aagatcgtgt ggcccgacgc tattgctacg
    82201 atgtttatcc attcttagcg aggctgcggt ggcgagttgc gtttttaact caccgtagag
    82261 tggtgcgaaa cgacggttaa aaccgaccat caacgtcagt tttttccgcg ccgccagttc
    82321 caccagcctt tcagcatcgc gcagattttc tgccagcggt ttatcgacac agacatgtac
    82381 ccccgcattg agtaacgtac tgaccacgtc aaagtggctg gcggtgctgg agtgcacaaa
    82441 aaccgcatca caactggcgg caaggctgga taacgaatcg gcataaggaa tgcgccagct
    82501 gtcacaaatt ggcagggctt tggcgcgcgt aggcgaccag gctccttgta acgtccagtc
    82561 agacgctgcc gccagcaccg gtaaccacgc tttttgcgca atgccgccta atcccactac
    82621 gccgatacgt aattttttca ctttaatctc ccagatgggc cagcaacgaa tcaagacgtt
    82681 gtttcagttc tgccacttcg atttccaggg cttcgacgcg ggtctgtaaa tcaccgtcaa
    82741 ccatgttcga catagccgtt accgccggct gatcttcaac ctcaccactg aaaagatgca
    82801 tgtagcggct ttcgcgttta cccggttcgc gggccagacg caccacaaaa ggaccatctt
    82861 cgcgattcgc cagttgttcc agcgtcgact ccacttccgc catatcgctg aattcataca
    82921 ttcgcgcggc gcggctgcgc agttcacccg gcgtctgggc accacgcaat aacaacgtgg
    82981 tgattaacgc cacttctgcg gcgctcagtt tcagatcgcc aaattctgaa ttacaaaaac
    83041 gttgctcata tttggtgacc cgattaccaa aaccgctcac tgtgcgtaga taatgacgtt
    83101 tgaccagatt atccagctgt tcctgcactt cggattcact cagattcatg accggttcac
    83161 ggttcgtttt ctgattacag gccgtgacta caccattgac tgagagcggg tattgctccg
    83221 gcgtggtcac ctgcttttcc agcaagcaac caattacgcg agcttccagg gcagtaagtt
    83281 gatatttcat gagattctcc ttagcggccg ggcgtccagt ctggggtagt taatgccgtc
    83341 agtacgtgat cgcgccattg tccatcaatc aacagatagt ctttcgcata gccttctttt
    83401 tcaaaaccca gtcgcgccag taaatcaccg ctgcgtttat tgtgcggcat ataattagcc
    83461 ataatgcgat gaatatgttg ggtgcgctgc atataacgaa tggctgcggt cagggcttca
    83521 aacatgagtc ctttgccctg ccatttttgc ccaatcgaat aaccgagata gcaggcatga
    83581 aaagagccgc gaacaacatt ggaaaaattg gcaacgccaa taatctcttt ttcgtccggg
    83641 tcgaataagc caaagtagaa agctgaacct tgtttatgaa attcgttaat catccctagc
    83701 ctggcctgcc agcctgatgg ataacagtgg ctttcgtcgc gcactggctc ccagggcttg
    83761 aggaaatggc gattctctgc gtaataatcc gccagacgcc aggcatcacg atcatgcacc
    83821 agacgcacga ccagtcggtc tgtggttaag cgcacttttg gcacgttact gcgatagcca
    83881 aacattctat acctactcct tcccgtaacg tctttaatac gctgtaagta ttactatacc
    83941 tgcgcaacca gaccctgtga aaagtccaaa atgccatttt ctgcattatt cacatatttg
    84001 ataaaaactc tctatcaaag cgacgttttg ataaaaaaat actgtccccg aagtgatggg
    84061 aaaagtctta gcggcaatgc agaatagaaa gtgctcatct tttatttccc gggaggggaa
    84121 atgtcccgcg tatcgcaggc gaggaacctg ggtaaatatt tcctgctcat cgataatatg
    84181 ctggtcgtgc tggggttctt tgttgtcttc ccgctgatct ctatccgctt cgttgatcaa
    84241 atgggctggg ccgccgtcat ggtcggtatt gctctcggtc tacgccagtt tattcagcaa
    84301 ggtctgggta ttttcggcgg cgcaattgcc gaccgctttg gtgccaaacc gatgattgtt
    84361 accggtatgc tgatgcgcgc cgccggattc gccacaatgg gtatcgccca cgaaccgtgg
    84421 ctattgtggt tttcatgcct gctctcggga ctcggcggca cgttgtttga tccgccgcgt
    84481 tcggcgctgg tggtgaaact aatccgtcca caacaacgtg gtcgtttttt ctcgctgttg
    84541 atgatgcagg acagtgccag tgcggtcatt ggcgcattgt tggggagctg gctgttgcaa
    84601 tacgactttc gcctggtctg cgccacaggg gccgttttgt ttgtgctatg tgcggcgttc
    84661 aatgcgtggt tgttaccggc atggaaactc tccaccgtac gcacgcccgt tcgcgaaggc
    84721 atgacccgcg tgatgcgtga caagcgtttt gtcacctatg tcctgacgct ggcgggttac
    84781 tacatgctgg ctgtacaagt gatgctgatg ctgccaatta tggtcaacga cgtggctggc
    84841 gcgccctctg ccgttaaatg gatgtatgcc attgaagcgt gtctgtcgtt aacgttgctc
    84901 taccctatcg cccgctggag tgaaaagcat tttcgtctgg aacaccggtt gatggctggg
    84961 ctgttgataa tgtcattaag catgatgccg gtgggcatgg tcagcggcct acaacaactt
    85021 ttcaccctga tttgtctgtt ttatatcggg tcgatcattg ccgagcctgc gcgtgaaacc
    85081 ttaagtgctt cgctggcgga cgcaagagct cgcggcagct atatggggtt tagccgtctg
    85141 ggtctggcga ttggcggcgc tattggttat atcggtggtg gctggctgtt tgacctgggc
    85201 aaatcggcgc accagccaga gcttccgtgg atgatgctgg gaattattgg catcttcact
    85261 ttccttgcgc tgggttggca gtttagtcag aaacgcgccg cgcgtcgttt gctggaacgc
    85321 gacgcctgat ttgatactcc cccctgactc ctccatactg aaagtgaact gacagccaaa
    85381 tggaggagtc acgtgaagct atacatttac gatcactgcc cttactgcct caaagcccgc
    85441 atgattttcg gcctgaaaaa tatccccgtc gaattacatg ttctgctcaa cgacgacgca
    85501 gaaacaccca cccggatggt cggtcaaaaa caggttccca ttctgcaaaa agatgacagc
    85561 cgctatatgc cagaaagcat ggatatcgtt cactatgtcg ataaactcga cggcaaaccg
    85621 ttactgaccg gcaaacgttc ccctgccatt gaagagtggc tgcgcaaggt caatggctac
    85681 gtcaacaaac tgctgttgcc gcgttttgcc aaatcggcat ttgatgagtt ttctactccc
    85741 gccgcgcgca aatatttcgt cgacaagaaa gaggccagcg cgggtaattt tgccgacctg
    85801 ctggcccact ctgacggtct gattaagaat atcagcgatg atttgcgtgc gctggacaaa
    85861 ctgatcgtca aaccgaacgc cgtgaatggc gaactttcgg aagatgatat tcagctattt
    85921 ccgctactgc gtaatctgac gctggtagcc ggaatcaact ggccaagccg cgttgctaat
    85981 taccgcgata atatggcgaa acagacacaa atcaatttgt tatcatcaat ggcgatttaa
    86041 tcctgacccg ccgcggcagc ctgtattcgc gggttgccgc cgttttcagt tttttctgct
    86101 ggcgagttta tcgctggtga cacatgctgc gttgtgtcag gatattgacg cagatttcat
    86161 gtcgaggact accatgaaca agtttttatt tgccgctgca ttgatcgtca gtggcctgct
    86221 cgttggctgt aatcaactca cccaatacac catcaccgaa caagaaatta accagtcgct
    86281 tgcgaaacat aataatttct caaaagatat cggtttaccc ggcgtggctg acgcccatat
    86341 tgttctgaca aacttgacca gccaaattgg tcgcgaagag ccgaataagg ttaccctaac
    86401 cggagacgcc aatctggaca tgaactccct gttcggtagt cagaaagcga ccatgaaact
    86461 gaagctgaaa gcgctgcccg tgtttgataa agaaaaaggt gcgatcttcc tgaaagagat
    86521 ggaagtggtc gatgcgacgg tacaaccgga aaaaatgcaa acggtgatgc aaacgttgct
    86581 tccctatttg aaccaggcat tacgcaatta ctttaaccag caacctgctt acgtcctgcg
    86641 cgaagatggc agtcagggcg aagcaatggc gaaaaaactg gcgaaaggca ttgaagtgaa
    86701 gccaggcgaa attgtcattc cgtttacgga ttaatgacga gggcgcattc gcgcccttta
    86761 tttttcgtgc aaaggaaaac gtttccgctt atcctttgtg tccggcaaaa acatcccttc
    86821 agccggagca tagagattaa tgactgcacc atcccaggta ttaaagatcc gccgcccaga
    86881 cgactggcac cttcacctcc gcgatggcga catgttaaaa actgtcgtgc cgtataccag
    86941 cgaaatttat ggacgggcta tcgtaatgcc caatctggct ccgcccgtga ccacagttga
    87001 ggctgccgtg gcgtatcgcc aacgtattct tgacgccgta cctgccgggc acgatttcac
    87061 cccattgatg acctgttatt taacagattc gctggatcct aatgagctgg agcgcggatt
    87121 taatgaagcc gtgttcaccg ctgcaaaact ttacccggca aacgcaacca ctaactccag
    87181 ccacggcgtg acgtcaattg acgcaatcat gccggtactt gagcgcatgg aaaaaatcgg
    87241 tatgccgcta ctggtgcatg gtgaagtgac acatgcagat atcgacattt ttgatcgtga
    87301 agcgcgcttt atagaaagcg tgatggaacc tctgcgccag cgcctgactg cgctgaaagt
    87361 cgtttttgag cacatcacca ccaaagatgc tgccgactat gtccgtgacg gaaatgaacg
    87421 gctggctgcc accatcactc cgcagcatct gatgtttaac cgcaaccata tgctggttgg
    87481 tggcgtgcgt ccgcacctgt attgtttacc catcctcaaa cgcaatattc accaacaggc
    87541 attgcgtgaa ctggtcgcca gcggttttaa tcgagtattc ctcggtacgg attctgcgcc
    87601 acatgcacgt catcgcaaag agagcagttg cggctgcgcg ggctgcttca acgccccaac
    87661 cgcgctgggc agttacgcta ccgtctttga agagatgaat gctctgcagc actttgaagc
    87721 attctgttct gtaaacggcc cgcagttcta tggcttgccg gtcaacgaca cattcatcga
    87781 actggtacgt gaagagcaac aggttgctga aagcatcgca ctgactgatg acaccctggt
    87841 gccattcctc gccggggaaa cggtacgctg gtccgttaaa caataaaaat cgtagcttcc
    87901 tgttgtcatt aggttatttt acctgtataa ataaccagta tattcaacag ggggctatta
    87961 tgcgaattga agtcaccata gcgaaaactt ctccattgcc agctggggct attgacgccc
    88021 tggctggcga actttcccgc cgtattcagt atgcgtttcc tgataatgaa ggccacgtat
    88081 cggtacgtta tgccgcagcg aataatttat cggttattgg cgcaacaaaa gaagataaac
    88141 agcgcattag cgaaattctc caggaaacgt gggaaagcgc cgatgactgg tttgtcagcg
    88201 aataatatgc agtgattttt gtttttgccg gatcgccccg gcttttttat tttacttacg
    88261 aattttgagt acaaaatttc tacacttcct caaaataatt gcacaatatg ctaaaaaaat
    88321 atgaatgaca tgctgtttta ttgttcatat aaatcttaaa agcagaaatt tatctgttcc
    88381 cccctttaaa aattcggtga atacccttac ttattggtat actgaagttg cttcagataa
    88441 aaacgcattg aacctcgaat aacgttgtct agtaacacga attagggggc catgatggaa
    88501 aaaaataatg aagtcattca gactcatccg ctcgtagggt gggacatcag caccgttgat
    88561 agctatgatg cgctgatgtt gcgtttgcac taccagaccc caaataagtc cgagcaggaa
    88621 gggactgaag ttggtcagac gctctggtta accactgatg ttgccagaca atttatttcg
    88681 atattagaag caggaatcgc caaaattgaa tccggtgatt tcccggtaaa cgagtatcgg
    88741 cgtcattaac atgacatgac catccaccca aaaggcacct caccggtgcc tttaccattt
    88801 ctgggttgta tatctgcatt cagttaatta ctctgcttaa accatgagag cagagagaaa
    88861 aaaatgaaat acgatctcat cattattggc agcggttccg taggcgctgc cgccgggtat
    88921 tatgcaaccc gcgccggttt aaacgtgcta atgaccgacg cccatatgcc accgcatcaa
    88981 cacggcagcc accacggcga tacgcgatta attcgccatg cttatggtga aggcgaaaag
    89041 tatgtcccgt tggtcctccg cgcgcaaacg ctgtgggatg aactttcccg ccacaacgaa
    89101 gatgatccca tttttgtacg ctctggtgtc attaacctcg gcccggctga ctccgcattt
    89161 ctcgccaacg tcgcccacag cgccgaacag tggcaactca acgttgaaaa gctcgatgcg
    89221 caagggatta tggcccgctg gccagaaata cgcgtcccgg acaactacat cggcttattt
    89281 gagactgatt ccggtttttt gcgcagcgaa ctggcgatta aaacctggat ccaactggcg
    89341 aaggaagcgg gctgtgcgca actgttcaac tgcccggtca ccgcaattcg tcatgacgat
    89401 gatggcgtaa ctattgaaac ggttgacggt gagtatcagg cgaaaaaagc gattgtctgc
    89461 gcgggaacat gggtaaaaga cctgctcccg gagctgcctg tccagcccgt acgtaaagta
    89521 tttgcctggt atcaggccga tggccgctat agcgtgaaga ataaattccc ggcgtttacc
    89581 ggtgaactgc ccaatggcga tcaatattat ggttttccgg cagaaaacga cgcgttgaag
    89641 attggcaaac ataacggagg ccaggttatc cattcagcgg atgaacgtgt tccgtttgcg
    89701 gaagtggcca gtgatgggtc agaagccttc ccgttcttgc gcaatgtatt gccgggtatc
    89761 ggttgctgcc tgtacggcgc tgcctgcacc tatgataatt cgcctgacga agattttatt
    89821 atcgataccc tacccggcca cgataataca ctgctcatta ccggcctgag tgggcacggt
    89881 tttaaatttg cgtcagtttt aggggaaata gctgccgatt ttgcgcaaga ccaaaaaagc
    89941 gattttgatt tgacgccatt caggctttcc cgcttccaat aatcatcatt acggcctcca
    90001 gaatgaggcc gtttttacag gagtatttaa tgcgtcggct gttgcactat ctcatcaata
    90061 atattcgcga gcatctgatg ctttatcttt ttctctgggg attgttggca attatggatc
    90121 tgatctatgt gttttatttt tagttttccg cactgacata aactgacaaa tatttaatct
    90181 ccctttcatt tagcgtgaaa aattgaatga gattattcct gcttctacag cactcaccag
    90241 acatctctga tgctgaaccc actaaactta tgaagattct ttcattatta attcaaaatt
    90301 attgaaataa aaataaatat cattcaattt gttatttttg tgttgcaatt cattctccga
    90361 cacgccattt tctttctctc gcaaattcgt attggatgca cttatgtcat tcacaaatac
    90421 ccctgaacgc tatggtgtta tatctgcggc cttccattgg ctaagcgcaa ttattgtcta
    90481 cggcatgttt gctttaggtc tgtggatggt cacgctcagt tattacgatg gctggtatca
    90541 caaagcaccc gaactgcata aaagcatcgg tattttgtta atgatggggc tggttattcg
    90601 cgttctgtgg cgtgtcatat ctccaccgcc gggtccgctg ccgagttatt cgcccatgac
    90661 tcgtcttgcc gcaaaagctg gacatctcgc attgtgccta ttgctgttcg ccatcggcat
    90721 cagcggctat ctgatctcaa ctgccgatgg taaaccgatc agcgtttttg gctggtttga
    90781 cgtccccgcg acccttgccg acgctggcgc acaggcagac tttgccggtg ccctgcattt
    90841 ttggctcgcg tggagcgtcg tagtactgtc tgttatgcac ggatttatgg ccctgaagca
    90901 tcatttcatc gataaagacg acactctgaa gcgcatgctg ggaaagtcgt catctgacta
    90961 tggagtataa aaaatgaaaa aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc
    91021 tgccggttca gcggttgccg ccgattacaa aattgacaaa gaaggtcagc acgcctttgt
    91081 taacttccgc atacagcatc ttggctatag ctggttatat ggcaccttta aagatttcga
    91141 cggtactttt acctttgatg aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa
    91201 caccaccagc gtcgatacta accacgccga acgcgataaa catcttcgca gtgcggattt
    91261 tctcaatacc acaaaatatc cacaggcaac attcacctcc accagcgtga agaaagacgg
    91321 tgacgaactg gatattaccg gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct
    91381 ggaagcgaaa ttaattggtc agggcgacga cccatggggc ggtaaacgtg caggctttga
    91441 ggccgaaggc aaaattaagc tcaaagactt caatatcaag acagatttag gtccagcttc
    91501 tcaggaagtg gatctgatta tttcagtgga aggcgtacag cagaagtaag agtgaaatgt
    91561 tgccggatgg cacgcatccg gcaatgatat ttattctgtt ggatcaggaa tacccagtgt
    91621 tgtattcaga cgtccacgag acttattaaa gatcttattg ccattttcac gtcctgcccg
    91681 acggcgtcgc tgttcttctg gcggtaacgc gctttcttcg cagcaaatct cactacaaca
    91741 acctttgtat ttttccgcgc atactggaca ctgaataaac agcaggtggc agccatcatt
    91801 tttacagttg gtatggctgt cgcacggcgc gccgcactgg tggcaatgcg cgataatctc
    91861 atccgatata cgttcgccca tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac
    91921 cggcaagccc tgctcgcgcg ccttacgggc gtattcaata attccgccct cgatatgcca
    91981 gactttgttg aatccgttat gtttcatcca ggcactggcc ttttcacaac gaatgccgcc
    92041 ggtgcagtac atgacgattt ttttatcttt atgtgcctgc atcatctcga ctgcttttgg
    92101 cagctgctca cggaaggtat ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc
    92161 atactcatag tggttacgca tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt
    92221 cacttccgcc gcttgcagat actcaccaac attgctggca tcaaagtgag gatcgtcaat
    92281 accgtcggca acaatgcgat cgcgtacctt catgcgcagt acccagaagg atttcccgtc
    92341 atcatccaac gcgatattca ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg
    92401 cgcgcgaaat gtttcaacat tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg
    92461 cgccagatac actcgcccaa aaacattcag cgcggtaaac agctgatata aagcgtcacg
    92521 ggtcgcctta ggatcggcga tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc
    92581 gctctcagcc aacattttgg cttttaacgc gtcgttggaa atgcggttgt gtaacactgg
    92641 catggtgtac ggttcctgcg agatgggaaa gtaaaaatcc gcggcatgat atagcaatta
    92701 tcgataatta acatccacac attttacgct acatttgcgc attaaaaatt atttgttatt
    92761 tacaagcgcg gcaatttcgc ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat
    92821 gcgacaatac atacaattgc ccgtataggt tgaaaaacag gattgatatg acgaatctac
    92881 ccaagttctc caccgcactg cttcatccgc gttattggtt aacctggttg ggtattggcg
    92941 tactttggtt ggtcgtgcaa ttgccctacc cgattatcta ccgcctcggt tgtggattag
    93001 gaaaactggc gttacgtttt atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac
    93061 tgtgcttccc ggaaatgagc gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat
    93121 ccgttggcat gggcctgatg gaaaccggta tggcgtggtt ctggccggac cgccgaatcg
    93181 cccgctggac ggaagtgatc ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg
    93241 gcatcctgtt agttggcatc cattttctga cactggagct gggcgcgcgg cagtttggta
    93301 tgcaggaacc ggggattggc gtttaccgcc cgaacgataa tccactgatt gactggctac
    93361 aaacctgggg ccgtttgcgc tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca
    93421 tgattaaagc cctgaaaaaa ggcgaagtgg tctggtacgc accagatcat gattacggcc
    93481 cgcgctcaag cgttttcgtc ccgttatttg ccgttgagca ggctgcgacc acgaccggaa
    93541 cctggatgct ggcacggatg tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc
    93601 cagatggcaa agggtatcaa ttgattatgc tgccaccaga gtgttctccg ccactggatg
    93661 atgccgaaac taccgccgcg tggatgaaca aagtggtcga aaaatgcatc atgatggcac
    93721 cagagcagta tatgtggtta caccgtcgct ttaaaacacg cccggaaggc gttccttcac
    93781 gctattaaat ctcccatgcc ggatgcttca gaatggcatc cggcattacc acagcaaatc
    93841 cccctgattt agcgataaaa gctctctgga ttgcgccccc tggaagtcgg gcgcataatt
    93901 agtgtgttta tcttttcttc ttatgttcac cgcgcctggc gcaccaacag cggattgcta
    93961 tgtcaccctg tgaaaatgac acccctataa actggaaacg aaacctgatc gtcgcctggc
    94021 taggctgttt tcttaccggg gccgccttca gtctggtaat gcccttctta cccctctacg
    94081 ttgagcagct tggtgttacc ggccactccg ccctgaatat gtggtccggt attgtcttca
    94141 gtataatggg ttcctaaaca catcatttta ggtattgaat atgagacggg caatcagcta
    94201 catcagattt tcaagtgaac gacaactgaa aggggactct gtacgccgac aatctaagct
    94261 ggttactgac tggcttgata agaaccctga gttttacttg gattcatcct tgagctttaa
    94321 agatttgggg aaaagtgctt ttagtggtaa gcacctgaaa ggtggtgtag gtgacttttt
    94381 aaccgctatc gagaaagggc ttgttaaagc tggtgatacg ctactaatcg agagtttaga
    94441 ccgtctgtca cgtcaggata ttgatattgc ttctgagcta ttacgccgta tcttaagggc
    94501 tggtgtagat gtagtcacac tgagcgatgg agaacattac acacgagaaa gtttaaaaga
    94561 cccgttagcc ctgattaaaa gcattctgat aatgcagcgg gcgcatgagg aaagtttgag
    94621 aaagtctgag cgtgtccaag ctgcatggaa ccgtaaaaaa gaactcatct ccgaaggtat
    94681 caaggtatct aggcgttgtc ctgcatggtt aaggctgaat gatgaccgtc gaacattcac
    94741 aatcattcct gataaagtgg aagttgttaa acgagcattt gacctgagat tacagggttt
    94801 aagcttttgg gctatcacca gaactttaaa cgatgaaggt cacttgtcat tgaatcaata
    94861 cacacccaaa caaaaggggt ggtctgatac tgctgtaaag aaactgctca gaaatcgtgc
    94921 tgtgattgga tgtttcacac ctgctggacg tgaagaagtt cagggttact accccgcaat
    94981 aatcagtgag tcactattct atcgagtaca gcaactgaac acagggcaat atggtagggc
    95041 ttctgtatca agtaatccgc tatcagtcaa cttgttcagg ggtatcatca a
//
LOCUS       DAJTSF010000003        75635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000003 DAJTSF010000000
VERSION     DAJTSF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.003"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(435..641)
                     /locus_tag="OO335_000237"
     CDS             complement(435..641)
                     /locus_tag="OO335_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6000771.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            955..1044
                     /gene="kdpF"
                     /locus_tag="OO335_000238"
     CDS             955..1044
                     /gene="kdpF"
                     /locus_tag="OO335_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6000772.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1044..2717
                     /gene="kdpA"
                     /locus_tag="OO335_000239"
     CDS             1044..2717
                     /gene="kdpA"
                     /locus_tag="OO335_000239"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR6000773.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2740..4787
                     /gene="kdpB"
                     /locus_tag="OO335_000240"
                     /pseudo
     CDS             2740..4787
                     /gene="kdpB"
                     /locus_tag="OO335_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4796..5368
                     /gene="kdpC"
                     /locus_tag="OO335_000241"
     CDS             4796..5368
                     /gene="kdpC"
                     /locus_tag="OO335_000241"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR6000774.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5361..8041
                     /gene="kdpD"
                     /locus_tag="OO335_000242"
                     /pseudo
     CDS             5361..8041
                     /gene="kdpD"
                     /locus_tag="OO335_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8038..8723
                     /gene="kdpE"
                     /locus_tag="OO335_000243"
                     /pseudo
     CDS             8038..8723
                     /gene="kdpE"
                     /locus_tag="OO335_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8998..9102
                     /locus_tag="OO335_000244"
     CDS             8998..9102
                     /locus_tag="OO335_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR6000775.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9413..11610
                     /gene="speF"
                     /locus_tag="OO335_000245"
                     /pseudo
     CDS             9413..11610
                     /gene="speF"
                     /locus_tag="OO335_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11607..12926
                     /gene="potE"
                     /locus_tag="OO335_000246"
     CDS             11607..12926
                     /gene="potE"
                     /locus_tag="OO335_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6000776.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12982..14622)
                     /gene="pgm"
                     /locus_tag="OO335_000247"
     CDS             complement(12982..14622)
                     /gene="pgm"
                     /locus_tag="OO335_000247"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6000777.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14648..15193)
                     /gene="seqA"
                     /locus_tag="OO335_000248"
     CDS             complement(14648..15193)
                     /gene="seqA"
                     /locus_tag="OO335_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6000778.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15378..16142
                     /gene="ybfF"
                     /locus_tag="OO335_000249"
     CDS             15378..16142
                     /gene="ybfF"
                     /locus_tag="OO335_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6000779.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16282..16575
                     /gene="ybfE"
                     /locus_tag="OO335_000250"
     CDS             16282..16575
                     /gene="ybfE"
                     /locus_tag="OO335_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6000780.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16715..17245
                     /gene="fldA"
                     /locus_tag="OO335_000251"
     CDS             16715..17245
                     /gene="fldA"
                     /locus_tag="OO335_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6000781.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17455..17541
                     /gene="uof"
                     /locus_tag="OO335_000252"
     CDS             17455..17541
                     /gene="uof"
                     /locus_tag="OO335_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6000782.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17534..17980
                     /gene="fur"
                     /locus_tag="OO335_000253"
     CDS             17534..17980
                     /gene="fur"
                     /locus_tag="OO335_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6000783.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18064..18390)
                     /locus_tag="OO335_000254"
     CDS             complement(18064..18390)
                     /locus_tag="OO335_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000784.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18427..19833)
                     /gene="chiP"
                     /locus_tag="OO335_000255"
     CDS             complement(18427..19833)
                     /gene="chiP"
                     /locus_tag="OO335_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR6000785.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20412..22076)
                     /gene="glnS"
                     /locus_tag="OO335_000256"
     CDS             complement(20412..22076)
                     /gene="glnS"
                     /locus_tag="OO335_000256"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6000786.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22279..24225)
                     /gene="nagE"
                     /locus_tag="OO335_000257"
     CDS             complement(22279..24225)
                     /gene="nagE"
                     /locus_tag="OO335_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6000787.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24558..25358
                     /gene="nagB"
                     /locus_tag="OO335_000258"
     CDS             24558..25358
                     /gene="nagB"
                     /locus_tag="OO335_000258"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6000788.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25418..26566
                     /gene="nagA"
                     /locus_tag="OO335_000259"
     CDS             25418..26566
                     /gene="nagA"
                     /locus_tag="OO335_000259"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6000789.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26575..27795
                     /gene="nagC"
                     /locus_tag="OO335_000260"
                     /gene_synonym="nagR"
     CDS             26575..27795
                     /gene="nagC"
                     /locus_tag="OO335_000260"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6000790.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27843..28595
                     /gene="nagD"
                     /locus_tag="OO335_000261"
                     /pseudo
     CDS             27843..28595
                     /gene="nagD"
                     /locus_tag="OO335_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            28992..30656
                     /gene="asnB"
                     /locus_tag="OO335_000262"
     CDS             28992..30656
                     /gene="asnB"
                     /locus_tag="OO335_000262"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6000791.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31035..31111
                     /locus_tag="OO335_000263"
     tRNA            31035..31111
                     /locus_tag="OO335_000263"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31069..31071,aa:Met,seq:cat)
     gene            31120..31204
                     /locus_tag="OO335_000264"
     tRNA            31120..31204
                     /locus_tag="OO335_000264"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31154..31156,aa:Leu,seq:tag)
     gene            31228..31302
                     /locus_tag="OO335_000265"
     tRNA            31228..31302
                     /locus_tag="OO335_000265"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31260..31262,aa:Gln,seq:ttg)
     gene            31337..31411
                     /locus_tag="OO335_000266"
     tRNA            31337..31411
                     /locus_tag="OO335_000266"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31369..31371,aa:Gln,seq:ttg)
     gene            31427..31503
                     /locus_tag="OO335_000267"
     tRNA            31427..31503
                     /locus_tag="OO335_000267"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31461..31463,aa:Met,seq:cat)
     gene            31552..31626
                     /locus_tag="OO335_000268"
     tRNA            31552..31626
                     /locus_tag="OO335_000268"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31584..31586,aa:Gln,seq:ctg)
     gene            31664..31738
                     /locus_tag="OO335_000269"
     tRNA            31664..31738
                     /locus_tag="OO335_000269"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31696..31698,aa:Gln,seq:ctg)
     gene            complement(31892..33067)
                     /gene="ubiF"
                     /locus_tag="OO335_000270"
     CDS             complement(31892..33067)
                     /gene="ubiF"
                     /locus_tag="OO335_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6000792.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33213..34637
                     /gene="miaB"
                     /locus_tag="OO335_000271"
     CDS             33213..34637
                     /gene="miaB"
                     /locus_tag="OO335_000271"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6000793.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34790..35830
                     /locus_tag="OO335_000272"
     CDS             34790..35830
                     /locus_tag="OO335_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6000794.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35827..36294
                     /gene="ybeY"
                     /locus_tag="OO335_000273"
     CDS             35827..36294
                     /gene="ybeY"
                     /locus_tag="OO335_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6000795.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36384..37262
                     /gene="corC"
                     /locus_tag="OO335_000274"
     CDS             36384..37262
                     /gene="corC"
                     /locus_tag="OO335_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6000796.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37287..38825
                     /gene="lnt"
                     /locus_tag="OO335_000275"
     CDS             37287..38825
                     /gene="lnt"
                     /locus_tag="OO335_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6000797.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39222..40130
                     /gene="gltI"
                     /locus_tag="OO335_000276"
     CDS             39222..40130
                     /gene="gltI"
                     /locus_tag="OO335_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR6000798.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40300..41040
                     /gene="gltJ"
                     /locus_tag="OO335_000277"
     CDS             40300..41040
                     /gene="gltJ"
                     /locus_tag="OO335_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR6000799.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            41040..41714
                     /gene="gltK"
                     /locus_tag="OO335_000278"
     CDS             41040..41714
                     /gene="gltK"
                     /locus_tag="OO335_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6000800.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41714..42439
                     /gene="gltL"
                     /locus_tag="OO335_000279"
     CDS             41714..42439
                     /gene="gltL"
                     /locus_tag="OO335_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6000801.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42557..43492
                     /gene="rihA"
                     /locus_tag="OO335_000280"
     CDS             42557..43492
                     /gene="rihA"
                     /locus_tag="OO335_000280"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6000802.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43576..45246
                     /gene="hscC"
                     /locus_tag="OO335_000281"
                     /pseudo
     CDS             43576..45246
                     /gene="hscC"
                     /locus_tag="OO335_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45306..46757)
                     /gene="djlC"
                     /locus_tag="OO335_000282"
     CDS             complement(45306..46757)
                     /gene="djlC"
                     /locus_tag="OO335_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR6000803.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46754..47461)
                     /locus_tag="OO335_000283"
     CDS             complement(46754..47461)
                     /locus_tag="OO335_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6000804.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47563..48117
                     /locus_tag="OO335_000284"
     CDS             47563..48117
                     /locus_tag="OO335_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6000805.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48127..49554)
                     /locus_tag="OO335_000285"
     CDS             complement(48127..49554)
                     /locus_tag="OO335_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6000806.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49551..50258)
                     /locus_tag="OO335_000286"
     CDS             complement(49551..50258)
                     /locus_tag="OO335_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6000807.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50422..51399
                     /locus_tag="OO335_000287"
     CDS             50422..51399
                     /locus_tag="OO335_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6000808.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51469..51951)
                     /locus_tag="OO335_000288"
     CDS             complement(51469..51951)
                     /locus_tag="OO335_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6000809.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52186..54768
                     /gene="leuS"
                     /locus_tag="OO335_000289"
     CDS             52186..54768
                     /gene="leuS"
                     /locus_tag="OO335_000289"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6000810.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54783..55364
                     /gene="lptE"
                     /locus_tag="OO335_000290"
     CDS             54783..55364
                     /gene="lptE"
                     /locus_tag="OO335_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6000811.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55364..56395
                     /gene="holA"
                     /locus_tag="OO335_000291"
     CDS             55364..56395
                     /gene="holA"
                     /locus_tag="OO335_000291"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6000812.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56397..57038
                     /gene="nadD"
                     /locus_tag="OO335_000292"
     CDS             56397..57038
                     /gene="nadD"
                     /locus_tag="OO335_000292"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6000813.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57062..57673
                     /locus_tag="OO335_000293"
     CDS             57062..57673
                     /locus_tag="OO335_000293"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR6000814.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57933..58250
                     /gene="rsfS"
                     /locus_tag="OO335_000294"
                     /gene_synonym="rsfA"
     CDS             57933..58250
                     /gene="rsfS"
                     /locus_tag="OO335_000294"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6000815.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58254..58721
                     /gene="rlmH"
                     /locus_tag="OO335_000295"
     CDS             58254..58721
                     /gene="rlmH"
                     /locus_tag="OO335_000295"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6000816.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58752..60653
                     /gene="mrdA"
                     /locus_tag="OO335_000296"
     CDS             58752..60653
                     /gene="mrdA"
                     /locus_tag="OO335_000296"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6000817.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60656..61768
                     /gene="mrdB"
                     /locus_tag="OO335_000297"
                     /gene_synonym="rodA"
     CDS             60656..61768
                     /gene="mrdB"
                     /locus_tag="OO335_000297"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6000818.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61779..62867
                     /gene="rlpA"
                     /locus_tag="OO335_000298"
     CDS             61779..62867
                     /gene="rlpA"
                     /locus_tag="OO335_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6000819.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            63006..64217
                     /gene="dacA"
                     /locus_tag="OO335_000299"
     CDS             63006..64217
                     /gene="dacA"
                     /locus_tag="OO335_000299"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6000820.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64328..64591
                     /locus_tag="OO335_000300"
     CDS             64328..64591
                     /locus_tag="OO335_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6000821.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64692..65333
                     /gene="lipB"
                     /locus_tag="OO335_000301"
     CDS             64692..65333
                     /gene="lipB"
                     /locus_tag="OO335_000301"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6000822.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65592..66545
                     /locus_tag="OO335_000302"
     CDS             65592..66545
                     /locus_tag="OO335_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6000823.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66754..67719
                     /gene="lipA"
                     /locus_tag="OO335_000303"
     CDS             66754..67719
                     /gene="lipA"
                     /locus_tag="OO335_000303"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6000824.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67820..68023)
                     /gene="tatE"
                     /locus_tag="OO335_000304"
     CDS             complement(67820..68023)
                     /gene="tatE"
                     /locus_tag="OO335_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6000825.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68152..68940)
                     /locus_tag="OO335_000305"
     CDS             complement(68152..68940)
                     /locus_tag="OO335_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6000826.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69033..69416
                     /gene="crcB"
                     /locus_tag="OO335_000306"
     CDS             69033..69416
                     /gene="crcB"
                     /locus_tag="OO335_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6000827.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIEPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69470..69679)
                     /gene="cspE"
                     /locus_tag="OO335_000307"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69470..69679)
                     /gene="cspE"
                     /locus_tag="OO335_000307"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6000828.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69854..70414)
                     /gene="pagP"
                     /locus_tag="OO335_000308"
     CDS             complement(69854..70414)
                     /gene="pagP"
                     /locus_tag="OO335_000308"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6000829.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            71003..72388
                     /gene="dcuC"
                     /locus_tag="OO335_000309"
     CDS             71003..72388
                     /gene="dcuC"
                     /locus_tag="OO335_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6000830.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(72429..72770)
                     /locus_tag="OO335_000310"
                     /pseudo
     CDS             complement(72429..72770)
                     /locus_tag="OO335_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73108..73617)
                     /locus_tag="OO335_000311"
     CDS             complement(73108..73617)
                     /locus_tag="OO335_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6000831.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73780..74025
                     /locus_tag="OO335_000312"
     CDS             73780..74025
                     /locus_tag="OO335_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6000832.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            74000..75631
                     /locus_tag="OO335_000313"
                     /pseudo
     CDS             74000..75631
                     /locus_tag="OO335_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        18614 a        18994 c        19212 g        18815 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt tgcttattga tggtttattt
      781 acattcacac tgtaattaaa ttacacaaaa gttaaattaa tactaaccat cagttaaatc
      841 atggcctttg ccatttttat acttttttta caccccgtcc gcagattttt gcgaaatctt
      901 tgcagccaga attctactct tccggtatca cttttaggcc actggaggtg cactgtgagt
      961 gcaggcgtga tagccggcgt gttgctggtg tttttattac tgggctattt ggtttatgcc
     1021 ctgatcaatg cggaggcgtt ctgatggctg cgcaagggtt cttactgatt gccacgtttt
     1081 tactggtatt aatggtgctg gcgcgtcctt taggcagcgg gctggcgcgg ctgattaatg
     1141 acattcctct tccaggtaca gcgggcgttg aacgtatact ctttcgttta cctggcgtct
     1201 ctgaccatga gatgaactgg aagcaatatc tttgtgccat tctcggcctg aacatgctgg
     1261 ggctggcggt gctgtttttt atgttgctcg gtcagcacta tctgccgctt aatccacagc
     1321 agttgccggg gctgtcgtgg gatctggcgc tgaataccgc cgtcagcttt gtcacgaata
     1381 ccaactggca atcttatagc ggtgaaacca cgttgagcta tttcagccag atggcaggct
     1441 taacggtaca aaactttctt tctgccgcca gcgggattgc ggtgattttt gccttcatcc
     1501 gtgcgtttac tcgccagagc atgagcacac tcgggaatgc ctgggtcgat ctgctacgca
     1561 tcacgttatg ggtgctagtc cctgtggcgt tgttgatcgc actgtttttt attcaacaag
     1621 gtgcgcagca aaactttctg ccctatcagg cagtgaatac cgttgaagga gcgcaacagc
     1681 tgttacccat gggaccagta gcttctcagg aagcgatcaa gatgctcggt actaacggcg
     1741 gtggcttctt taatgccaac tcgtcgcatc cgtttgaaaa cccaaccgca ctgaccaact
     1801 tcgtgcagat gctggcgatc ttcttgatcc caacagcgct gtgctttgcc tttggtgaag
     1861 tgacgggcga tcgccgccag gggcgcatgt tgctgtgggc gatgtcagtg atttttgtca
     1921 tctgcgtagg cgtggttatg tgggcagaag ttcagggtaa tcctcatctg ctggcgctcg
     1981 gcgcagacag cagcatcaat atggaaggta aagagagccg tttcggcgtg ctggtcagta
     2041 gcctgtttgc ggtcgtgact acggcggcgt cctgtggcgc ggtgattgcg atgcatgatt
     2101 cgtttaccgc tctcggcggc atggtgccga tgtggctaat gcaaattggt gaagtggtgt
     2161 tcggcggtgt cggttctggt ctttacggca tgatgctgtt tgtcctgctg gcggtgttta
     2221 ttgccgggct gatgattggt cgtacaccgg aatatctggg taaaaaaata gacgtacgcg
     2281 agatgaaact gactgcactg gcgattctgg ttactccaac actggtactg atgggcgcgg
     2341 cgttggcgat gatgaccgac gccggacgta gcgccatgct taaccctagc ccgcatggtt
     2401 ttagcgaagt gctgtacgcc gtgtcgtccg ccgctaacaa caacggcagc gcctttgccg
     2461 gattaagcgc caactctccg ttctggaact gtttactggc gttctgcatg tttgtcggtc
     2521 gcttcggggt gattatcccg gtgatggcaa ttgccggttc gctggtgagt aaaaagagcc
     2581 aacccgccag ctccggcacg ctgccaacgc acggcccgct gtttgttggc ctgttaatcg
     2641 gcaccgtgtt gctggttggc gcactgacct ttatccctgc cctggcgctt ggtccggtgg
     2701 cggaatatct ctcctgatga tattgagtga gcactgaata tgagtcgtaa acaactggcg
     2761 ctattcgaac caacacttgt cgttcaggcg ctgaaagaag cggtgaaaaa attaaacccg
     2821 caggcgcaat ggcgcaatcc ggtgatgttt atcgtctgga tcggcagtct gctgaccacc
     2881 tgtattagca tcgcgatggc aagcggtgcg atgcctggca atgcgctgtt tagcgcggca
     2941 attagcggct ggctgtgggt caccgtactg ttcgctaatt tcgccgaggc gctggcagaa
     3001 ggccgcagta aagcgcaggc caacagtctt aaaggggtga aaaaaactgc ctttgcccgc
     3061 aagctgcgtg agccgaaata tggcgctgcg gcggacaaag ttcctgccga tcaactgcgt
     3121 aaaggtgaca tcgtgctggt agaagctggc gatattatcc cctgcgatgg tgaagtcatt
     3181 gaaggcggcg catcggtcga tgaaagcgcc atcaccgggg aatcggcacc ggtgatccgt
     3241 gaatccggcg gcgattttgc ctccgttacc ggcggcacgc gtattctttc tgactggctg
     3301 gtgattgagt gtagcgttaa ccccggtgag acatttctgg atcggatgat cgcgatggtg
     3361 gaaggcgcac agcgacgcaa aacaccgaac gagattgccc tgactatcct gctgattgct
     3421 ctgaccatcg tctttttact ggcaaccgcc acgctgtggc cgttttccgc gtggggcggt
     3481 aatgccgtga gtgtaacggt actggtggcg ctgctggtct gtctgatccc aaccactatt
     3541 ggtggcctgt tgtcagcgat cggcgtcgcc gggatgagtc ggatgctggg cgcgaatgtg
     3601 attgccacca gcggacgtgc agttgaagcg gcaggtgacg ttgacgttct gctactggat
     3661 aaaaccggca ccatcacact cggtaaccgt caggcgtcgg agtttatccc cgcgcagggc
     3721 gtggatgaaa aaacgctggc tgacgccgca caactggctt cgctggctga tgaaacgccg
     3781 gaaggtcgca gtattgtgat cctcgccaag caacgtttta acctgcgcga gcgcgatgtg
     3841 cagtcgctcc atgccacctt tgtaccgttt actgcgcaaa gccggatgag cgggatcaac
     3901 atcgacaacc gcatgatccg taaaggttct gtcgatgcca ttcgccgcca tattgaggct
     3961 aacggcggtc acttccctgc cgatgttgat caaaaagtcg atcaggttgc gcgtcaagga
     4021 gccacgctgc tggtggtggt ggaaggttct cgtgtgctgg gcgttattgc gctgaaagat
     4081 atcgtcaaag gcggtattaa agagcgcttc gcccagctgc gcaaatgggc attaaaacgg
     4141 tgatgattac cggcgataac cgtctgactg ccgccgcgat agctgcggaa gcgggtgtcg
     4201 atgattttct cgccgaagcg acaccggagg ccaagctggc attgatccgt cagtatcagg
     4261 cggaaggtcg tttggtagcg atgaccggcg acagcaccaa cgatgctacg gcgctggcgc
     4321 aggcagatgt cgcggtggcg atgaactccg gcacccaggc ggctaaagag gcgggcaata
     4381 tggtggatct cgactctaac ccgaccaagt tgatcgaggt ggtgcacatt ggcaaacaga
     4441 tgctgatgac ccgtggctcg ctgaccacct tcagcattgc caacgatgtg gcgaaatact
     4501 tcgccattat tccggcggcg ttcgcggcaa catatccgca gttaaatgcg ctgaacatca
     4561 tgcgcctgca ttcgcccgac tccgccattc tcagtgcggt cattttcaac gccttgatta
     4621 tcgtcttttt gattcctctg gcgttaaaag gcgtgagtta taaaccgctt accgcttctg
     4681 ccatgttgcg ccgtaactta tggatttacg gtctgggtgg gctgctggtg ccgtttattg
     4741 gtatcaaagt cattgattta ctgctaaccg tttgtagtct ggtgtgaggt ttaccatgag
     4801 tggattacgt ccggcattat cgacatttct ctttctgtta ttgattactg gcggcgttta
     4861 cccgctgctg accaccgcac tggggcaatg gtggtatccc tggcaggcca acggttcgtt
     4921 gattcgtgaa ggtgatacgg tgcgcggttc ggcattaatc gggcaaaatt ttaccggcaa
     4981 cggctatttt catggtcgcc cgtcggcaac agcagaaatg ccctataatc cacaggcttc
     5041 tggcgggagc aatctggcag tcagtaaccc ggagctggat aaacagatag ccgcacgcgt
     5101 tgccgcatta cgggccgcta acccggacgc cagcacgaac gttccggttg aactggtgac
     5161 ggcgtcggca agcggactgg acaataacat caccccgcaa gcggcggcct ggcagatccc
     5221 acgcatcgca aaagcgcgta atctcagcgt tgaacagctc acgcagctga tcgcaaaata
     5281 cagccaacaa ccgctggtga aatatatcgg ccagccggtc gtcaacattg tcgaactcaa
     5341 tctggcactg gataagcttg atgaataacg aacccttacg tcccgacccc gaccgtctgc
     5401 tggaacaaac tgccgcgccg catcggggga agctgaaagt tttcttcagt gcctgtgcag
     5461 gcgtcgggaa gacctgggcg atgctggcag aagcccagcg actgcgggcg caagggctgg
     5521 atattgtggt tggtgtggta gaaacccatg ggcggaaaga taccgccgcc atgcttgaag
     5581 ggctggctgt tctgcctctg aaacgccagg cgtaccgtgg gcggcatatc agcgagtttg
     5641 atctcgatgc cgccctcgcc cgccgtccgg cgctgatctt aatggacgaa ctggcgcaca
     5701 gtaatgcgcc aggttctcgt catcctaaac gctggcagga tatcgaagaa ctgctggaag
     5761 ctggcattga tgttttcaca accgtcaacg ttcagcatct ggaaagtctg aatgatgtgg
     5821 tcagcggcgt caccggaatt caggtacggg aaaccgtgcc cgatcctttt ttcgatgccg
     5881 ccgacgacgt ggtgctggtg gacttgcccc cggacgatct gcgccagcgg ctgaaagaag
     5941 gcaaagtcta tattgccggg caggcggagc gcgccattga acattttttc cgcaaaggca
     6001 atctgatcgc cctgcgcgaa ctggcactgc gccgtactgc cgatcgcgtt gatgagcaaa
     6061 tgcgcgcctg gcgggggcat cctggcgaag agaaagtctg gcacacgcgc gacgcgatcc
     6121 ttttatgcat cggccataac accggcagcg aaaactggtc cgcgcggcgg cgcggctgac
     6181 gtcacggctg gggagcgtct ggcacgcggt gtatgttgaa acccctgccc tgcatcgctt
     6241 accggagaaa aaacgtcggg caattctcag cgccttacgt ctggcgcagg agctgggcgc
     6301 ggagacggca acactttctg atccagcgga agagaaagcg gtagtgcgtt atgcccgtga
     6361 acataatctc ggcaagatta ttctcggtcg cccagcctcg cgccgctggt ggcgtcggga
     6421 aacatttgct gaccgactgg cgcgcatcgc ccccgatctc gatcaggtgc tggtcgcgct
     6481 tgatgaacca cccgcccgca cgattaacaa cgcgccggat agccgctcat ttaaagacaa
     6541 gtggcgggta cagattcagg gatgcgtggt tgccgccgcg ttatgcgccg ttatcacctt
     6601 aattgccatg cagtggctga tggcgtttga tgccgccaac ctggtgatgc tgtatctgct
     6661 tggcgtggtg gtggcgctat tttatggacg ctggccttcg gtggttgcca ccgtcattaa
     6721 tgtggtgagt ttcgatctct tttttatcgc cccacgcggc acgctcgccg tctctgatgt
     6781 gcaatatctg ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac
     6841 tgctggtgtg cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca cacggcactt
     6901 atatgaaatg tcgaaagctc tggcggtggg ccgcagtccg caggacatcg ccgccaccag
     6961 cgaacaattt attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa
     7021 cggtaaattg cagccgttaa cacatccgca aggaattacg ccatgggacg atgccatcgc
     7081 gcagtggagt tttgataaag gcctgcctgc gggcgcgggc accgacacgt tacccggtgt
     7141 accgtaccag attttgccgc taaaaagcgg cgagaaaacc tacgggctgg tggtggtgga
     7201 gccggggaat ctgcgccagt tgatgatccc ggaacagcag cgcctgctgg agacttttac
     7261 gctgttagtc gccaatgccc ttgagcggct gacgctaacc gccagcgaag aacaggcgcg
     7321 gatggcaagc gaacgtgaac agatccgcaa cgccctgctg gcagcgcttt cgcatgattt
     7381 acgcacgccg cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag
     7441 cgaaggatca ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac
     7501 ccgactggtg aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa
     7561 gaaagagtgg ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc cggaaccggg
     7621 tttatcgtcg cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg
     7681 gccactcttt gaacgggtgc tgattaatct gctggagaac gcggtgaaat atgcgggtgc
     7741 gcaggccgaa attggtatcg atgcccacgt tgagggcgaa aatctacaac tggatgtctg
     7801 ggataacggc cccggtcttc cgccaggcca ggagcagacg atatttgata agtttgctcg
     7861 cgggaataaa gagtcggcgg tgccgggtgt cgggcttggg ttggcaattt gtcgggcgat
     7921 tgtggatgta cacggcggca ccattaccgc gttcaaccga ccggaaggtg gtgcctgttt
     7981 tcgtgttaca cttccccagc aaaccgctcc tgaacttgaa gactttcatg aggatatgtg
     8041 acaaacgttc tgattgttga agatgaacag gctattcgtc gctttctgcg cacggcgctg
     8101 gagggcgacg ggatgcgcgt ctttgaggcc gaaactctgc aacgcggctt gctggaagcg
     8161 gcaacccgta agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt
     8221 gagtttatcc gcgacctgcg ccagtggagc ccggtgccgg tgattgtgct ttccgcacgc
     8281 agcgaagaga gcgacaagat cgccgcgctg gatgccggag cggatgatta tctgagtaag
     8341 ccgtttggta ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc
     8401 actccgccac cgccgcgccc gatccgctgg tgaaattttc cggtgttacc gtcgatttag
     8461 ccgcccgcgt gattcaccgg ggtgaggaag aggtgcatct cacaccaatt gagttccgcc
     8521 tgctggcggt gctgctcaac aatgccggaa aagtactcac ccagcgccag ctccttaacc
     8581 aggtgtgggg gccaaacgcg gtcgaacaca gtcactattt gcgtatttat atgggacatc
     8641 tgcgacaaaa actggaacag gatccggccc ggcctcgcca cttcattact gaaaccggta
     8701 ttgggtatcg gtttatgctt tgaattgtta ttcaataaat tcgcttaaat agtgattaat
     8761 ttattattaa acctgcgccg aatattatta tttggcaggt ttaatttctt aacgcaaata
     8821 taaaacacga aattacaaca tttaagtaac aaaacagcat ctaatatggc gatattcata
     8881 attaatgatt tattatctgg ggtatgatcg ttttttgttg atcttcttca cagattatag
     8941 ccatttcatg gatagaataa ctctaccttc aactgacaca gcaagcggta aaggtaaatg
     9001 gaaaataata accgcttaat gcctcatata aggcggacaa cccatatcat gaagtttgcc
     9061 catcgtaata gttttgactt tcatttcttt aatgcccgct agtcttctga ctaaagggca
     9121 ccccaacgta caggtctccc tgactttaag cattacaggt taatacctgt attcctcggt
     9181 gctcatatac tgctaaccct tttaaactct aaataattcg agtcgcagca cttgcaactt
     9241 gaggtatgac gagtatagcc agttaccggg ctggtctggg ttattgcatc tgcaaaaagc
     9301 aaactactga tttatttatc agcggtggag ctttgctttt tttccgacgt gatcgatttc
     9361 tcctttgaga aattgaggac ctgctattac ctgaaataaa gagatgaata aaatgtcaga
     9421 attaaaaatt gccgttagcc gttcttgccc ggattgtttt tccactcatc gtgcatgcgt
     9481 gaatattgac gaaagtaatt atattgacgt tgccgccatt attttatcag tcaatgatgt
     9541 tgaacgtgga aaactcgata aaatagacgc tactggctat ggcattcctg tttttattgc
     9601 aacggaaaat gaagaacgtg taccagcaga atatctgcca cgtatttctg gtgtcttcga
     9661 gcattgcgaa tcacggaaag agttttatgg acgtcagtta gaaaccgctg ctagccatta
     9721 tgaaacgcaa ctgcgcccgc ccttcttccg cgcactggtc gattatgtca atcagggcaa
     9781 tagcgctttt gactgcccgg gtcatcaggg aggtgagttt ttccgtcgtc atccggcagg
     9841 gaatcagttc gtggagtact tcggagaaac gctgttccgt tccgacttat gcaacgcaga
     9901 cgtagcaatg ggcgatctgc tgattcacga aggcgcgcca tgcattgcac agcaacatgc
     9961 ggcaaaagtg tttaatgccg ataaaacata cttcgttcta aatggcactt catcttctaa
    10021 caaagtggtt ttaaacgccc tgctaacacc gggtgatctg gtgctgtttg atcgcaataa
    10081 ccacaaatct aaccaccacg gagcgttgct acaggctggt gcaacaccgg tttatctgga
    10141 aacggcacgt aacccgtatg gctttatcgg tggcattgat gcgcactgtt ttgaagaaag
    10201 ttacctgcgt gagctgatcg cggaagtcgc accgcagcgg gcaaaagagg ctcgtccttt
    10261 ccgcctcgct gtgattcagt taggcaccta cgacggtacg atttataacg cccgccaggt
    10321 ggtggataaa attggtcatc tgtgtgacta cattctgttt gactccgcat gggttggcta
    10381 tgagcaattt atcccgatga tggctgactg ttcgccgctg ttgctggatc ttaatgagaa
    10441 cgatccgggt attctagtta cgcaatccgt acataaacag caggctggtt tttctcaaac
    10501 ttcacaaatc cataaaaaag acagccacat caaagggcaa cagcgttatg taccgcacaa
    10561 acgcatgaac aacgccttta tgatgcacgc ctccaccagc ccgttctatc cgctgtttgc
    10621 cgcactggat atcaacgcca aaatgcatga aggtgtcagc ggtcgtaata tgtggatgga
    10681 ttgtgtggta aatggcatta atgcccgcaa actgatcctc gataactgtc agcatattcg
    10741 tccgttcgta cctgaactgg tggatggtaa accctggcag tcgtatgaaa cagcgcaaat
    10801 tgcggttgat ctgcgcttct tccagtttgt accaggggaa cactggcatt cttttgaagg
    10861 ctatgcagag aatcaatact ttgtcgatcc ttgcaaactg ttgctgacaa ccccaggtat
    10921 tgatgcacgt aacggcgaat atgaagcgtt cggtgtaccc gcgacgattc ttgctaactt
    10981 cctgcgcgaa aatggcgtag tgccggaaaa atgcgatctt aacgccatcc tcttcctgct
    11041 gactccggca gaagatatgg ccaaacttca gcaacttgtg ccctgctggt acgcttcgaa
    11101 aaactgcttg agtccgacgc gccattagca gaagtgctgc cttccatcta caaacagcat
    11161 gaagagcgct acgccggtta taccctgcgt cagttgtgcc aggaaatgca tgatttgtat
    11221 gcccgccaca acgtgaaaca actgcaaaaa gagatgttcc gcaaagcgca cttcccacac
    11281 gtcagcatga atccgcaaga agccaactac gcctatttac gcggtgaagt ggagctggtt
    11341 cgtctaccgg atgccgaagg tcgtatcgct gccgaagggg cgcttcctta tcctccgggt
    11401 gtgctgtgtg ttgttccggg tgaaatctgg ggtggtgctg ttttgcgtta cttcagcgct
    11461 ctggaagaag ggatcaacct gctgccaggt tttgcaccgg agctgcaggg tgtctatatc
    11521 gaagaacatg atggtcgtaa gcaagtttgg tgctatgtca tcaagcctcg tgatgcgcaa
    11581 agcaccctgt tgaaagggga aaaattatga gtcaggctaa atcgaacaaa atgggcgtcg
    11641 ttcagttaac catactgacg atggtcaaca tgatgggctc cggtatcatc atgctgccga
    11701 caaagcttgc cgaagttggg acaatttcaa ttatctcctg gctggtgaca gccgtaggct
    11761 caatggcact ggcatgggca ttcgcaaaat gcggtatgtt cagccgtaaa tcaggtggta
    11821 tgggcggtta tgccgagtat gctttcggta aatccggtaa ctttattgcg aactatacct
    11881 acggcgtctc actgctgatt gctaacgtcg cgattgctat ttcggcggtt ggttacggca
    11941 ccgaactgct cggcgcgagt ttgtcgccag tgcagattgg tcttgcgacc atcggggtgc
    12001 tgtggatttg taccgtggct aactttggtg gtgcgcgcat taccgggcaa atcagtagca
    12061 ttaccgtgtg ggggggcatt attccggtgg taggcctgtg cattatcggc tggttctggt
    12121 ttagcccgac gctttacgtt gattcctgga atccgcatca tgcaccgttc ttcagtgcag
    12181 taggttcttc catcgctatg acgctgtggg cttttcttgg tctggagtct gcgtgtgcga
    12241 atactgatgt agtggaaaac ccggaacgta atgtgccaat cgcggtactc ggcggtacgt
    12301 taggtgcggc ggtgatttat atcgtctcca ccaacgtgat tgcagggatt gtgcctaata
    12361 tggagctggc aaattcaacg gcaccatttg gcctggcctt cgcacagatg tttacaccag
    12421 aagtgggtaa agtcattatg gcgctgatgg tgatgtcctg ctgcggttcg ctgcttggct
    12481 ggcagttcac cattgcccag gtgtttaaat cttcatctga tgaaggctac ttccctaaaa
    12541 ttttctcccg tgtaaccaaa gtggatgcac cggtgcaggg aatgttgacc attgtgatta
    12601 tccagagcgg gttggcgctg atgacgatta gcccgtcgct aaacagtcag ttcaacgtgc
    12661 tggttaacct ggccgtggtg accaatatca ttccgtatat tctgtcaatg gcggcattag
    12721 tcattattca gaaggtcgct aatgtgcccc cctcaaaagc gaaagttgca aactttgttg
    12781 cttttgttgg cgcgatgtat agcttttatg cgctgtactc atccggggaa gaagccatgc
    12841 tgtacggttc catcgtgacc ttccttggtt ggacactgta tggtctggta tcaccacgct
    12901 ttgaactgaa aaataaacac ggttaaaaaa gcaaagtaaa aaagggcgat cttgcgaccg
    12961 ctcttttttt attaaatgtg tttacgcgtt tttcagaact tcgctaacaa tctcaaccgc
    13021 ttctttctca atctgcttgc gatgttcttc accgaggaag ctttcgcagt agatcttata
    13081 tgcgtcttcc gtgcctgacg gacgcgcggc gaaccagccg ttgtcagtca tcactttcag
    13141 accgccaata gaagcaccgt tgcccggagc agcagtcagg cgcgcggtga tcgggtcacc
    13201 tgccagggtg ctggcgctca ccatttccgg agacagctta gacagcgccg ctttttgtgc
    13261 ggaagtcgca gctgcctgca aacggttgta gctcggcgca ccaaagcgtt ttgccagttc
    13321 gttgtagtgt tcctgcgggt tcttaccggt gacagcggtg atttccgccg ccagcagaca
    13381 cataatgatg ccgtctttgt cggtggacca cggcgtgccg tcgaaacgca ggaaggaagc
    13441 ccctgcgctc tcttcgccgc caaagccgaa gctgccgtcg aacagaccat caacaaacca
    13501 tttgaaacct accggtactt ctaccagctt acggcccaag tcattgacca cacggtcgat
    13561 catcgcagaa gaaaccagcg ttttaccgac agcaacatct ttgccccact gcggacgatg
    13621 ctggaacagg tagttgatcg ccaccgccag gtagtggttc ggattcatca aacctgccgg
    13681 agtgacgata ccgtgacggt cataatccgg gtcgttagca aacgccagat cgaacttatc
    13741 acgcagtgcc agcagacccg ccatcgcaca ctcggaggag cagtccatac ggatcgcacc
    13801 gtctttatcg aggtgcataa agcggaaggt ttgatcgacc tgatcgttaa cgatggtcag
    13861 gttgaggttg taatactcgc caatgcgctt ccagtattcg ataccggaac cgcccaacgg
    13921 atcaacgccc agcgtcaggc ccgctttctg aatcgccgcc atatcaacga tatcggccag
    13981 cccttccacg aacggctgca ccagatcctg ctctttcaca tagccggatg ccatcgcttc
    14041 gtcgagggag atacgcttca cgcctttcag gccatcggcc atcagtgcgt tggccttgtc
    14101 ttccaccact ttggtgacgt tagtatcagc cgggccacca tttggcggat tgtacttgat
    14161 accaccatct tccggcgggt tatgggacgg tgtaatcacg ataccgtctg ccagcgggcc
    14221 acctttttta ttgtgaacca ggatggcatt ggaaacggca ggcgtcggag tgaagccatt
    14281 gttttcctgc acaatgacat caacgccgtt cgctgccagc acttccagta cggaaatgaa
    14341 cgcaggctcg gacagggcgt gagtatcttt acccacatag caagggccag tgatgccgtt
    14401 tttcgcacgt tcttcagcaa ttgcctgagc gatcgccaga atgtgcggct cgttaaagct
    14461 gtggcgcgct gcactgccac ggtgaccgga agtaccgaat ttcaccgcgt gctccgcatt
    14521 ccctgcttct ggtttcagta cataatattg cgccgtcagt tgggcgacgt taatcaaatc
    14581 actctgttgt gcaggttggc ctgcacgatt gtggattgcc attgctttgt cctttgtctg
    14641 caacgtttta gatagttccg caaaccttct caatcaattc cgccgggaat tgcatcgact
    14701 gcatgatgtg ttcgatcatg ctgcatttac ggccggtgtt ggtgttggtg atcacccaat
    14761 acggcgtgcc tggcacatgt ttcggcttgg tctgattacc atttttcagc agcgtttgtt
    14821 catctgccgc aaagtacacg cgtgtacgac cgtgcaacga ttccgttgct tcggcaaacg
    14881 cctgggcgtc aagagaatat agtgtagaca acagcagcat aaagcgattg accgctcgct
    14941 tttgctctgc gtattcgtcc gaaagcagaa gttcacgcat tgcgcgaacc ttgtctttaa
    15001 tcgttttgac cggcttcgct tcgacgatag caggtgacgc aacgcgaacc tctttcgtca
    15061 ccggagcagc aggctgtgat gcggcggaaa atttcaacat acgccgtaaa atgtcggatg
    15121 cgctctcgcc gatatgctta gtgtggctgg caatatagct gtagagttca tcatcaactt
    15181 caatcgtttt catcttaatc cagtgcagtg tcttagctga atacaagtcg ccaagagtat
    15241 atgaataaat cccggcagcg gatagcgtca agcctggctt gcagaaaaac tcggaataaa
    15301 ccttatttgt tgcagccctg tggcacaatg gtcaacataa taccctaacc cgacaacgcg
    15361 aaaaaaagaa ctttgccatg aaattaaata tccgcgcgca aactgcacaa aaccagcaca
    15421 ataattctcc catcgttctt gtccatggtc tgtttggtag cctcgacaac cttggcgtac
    15481 tggctcgcga tctggtaaac gatcacaata tcatccaggt tgatatgcgt aaccacggtc
    15541 tttcaccgag agagccggta atgaattacc cggcgatggc gcaggatctt gttgataccc
    15601 tggatgcact gcagatcgac aaagcaacat ttatcggtca ctccatgggc ggtaaagcgg
    15661 taatggcact tactgcactg gcccccgatc gcatcgataa actggtggcg atcgatatcg
    15721 caccggtcga ctatcacgta cgccgtcatg atgagatttt cgcagctatc aacgcggtca
    15781 gtgaatcgga cgcacaaact cgccagcaag cagcagcaat aatgcgccag catcttaatg
    15841 aagaaggggt gattcagttt ctgttgaaat cttttgttga cggggagtgg cgctttaacg
    15901 tgccggtatt gtgggatcag tatccgcata ttgtgggttg ggagaaaatc ccggcatggg
    15961 atcaccctgc cctgtttatc cctggcggca attctccgta tgttagcgag cagtaccgtg
    16021 atgatttact ggcacaattt ccacaggcac gggcgcatgt gattgcaggc gcgggtcact
    16081 gggtccatgc tgaaaaaccg gatgcggtat tacgcgctat ccgtcgctat ctcaatgatt
    16141 aacgcaactg attaaaaacc cagcgtccgc gtcgctttcc gcgcgtgggc gttggcgtga
    16201 ctcatcgcgc tgatgtatta tggcgcgcta tccatccggg cagaggcctg gctgattgtt
    16261 tcccccgaag tcaccaagat catggccaaa gaacaaacgg accgtacgac attagatctg
    16321 ttcgcgcacg agcgtcgacc gggacgaccg aaaactaatc cgctttcgcg cgatgaacag
    16381 ctgcgtatta ataaacgcaa ccagctaaaa cgcgacaaag tacgtggcct taagcgtgtc
    16441 gaactgaagc tgaacgcgga agctgtcgag gcgctgaacg agctggcgga atcgcgtaat
    16501 atgagccgta gcgaactgat cgaagagatg ttaatgcagc aactggcggc gctgcgtagt
    16561 cagggcatcg tttaaatttc cactttcatg tagcacagtg tgcagtcctg ctcgtttgct
    16621 gattccgcaa gactgcctgt tctgctatga ttgcctttat ccgtgggcaa ttttccaccc
    16681 ccatttcaat aagtttcaag aggttatttc actcatggct atcactggca tctttttcgg
    16741 cagcgacacc ggtaataccg aaaatatcgc aaaaatgatt caaaaacagc ttggtaaaga
    16801 cgttgccgat gtccatgaca ttgcaaaaag cagcaaagaa gatctggaag cttacgacat
    16861 tctgctgctg ggcatcccaa cctggtatta cggcgaagcg cagtgtgact gggatgactt
    16921 cttcccgact ctcgaagaga ttgatttcaa tggtaaactg gttgcgctgt ttggttgtgg
    16981 tgaccaggaa gattacgccg aatatttctg cgacgcattg ggcaccatcc gcgacatcat
    17041 tgaaccgcgc ggtgcaacca tcgttggtca ctggccaact gcgggctatc atttcgaagc
    17101 atcaaaaggt ctggcagatg acgaccactt tgtcggtctg gctatcgacg aagaccgtca
    17161 gccggaactg accgctgaac gtgtagaaaa atgggttaaa cagatttctg aagagttgca
    17221 tctcgacgaa attcttaatg cctgatgtga tgcggcgtag actcatgtct acgccgtatt
    17281 aatagataat gccaatcaaa ataattgcta caaatttgta acttttgctg ttgtacctat
    17341 acaatgtccc agtgttcaag tggccttgcc gttgtaaatg taagctgtgc cacgttttta
    17401 ttaacaatat ttgccaggga cttgtggttt tcatttaggc gtggcaattc tataatgata
    17461 cgcattatct caagagcaaa ttctgtcact tcttctaatg aagtgaaccg cttagtaaca
    17521 ggacagattc cgcatgactg ataacaatac cgccctaaag aaagctggcc tgaaagtaac
    17581 gcttcctcgt ttaaaaatcc tggaagttct tcaggagccg gacaaccatc acgtcagtgc
    17641 ggaagattta tacaaacgtc tgatcgatat gggtgaagaa attggtctgg ctacggtata
    17701 tcgcgtactg aaccagtttg acgacgctgg tatcgtcacc cgccacaatt ttgaaggcgg
    17761 taaatccgta tttgaactga cacagcaaca tcaccacgat cacctgattt gcctcgactg
    17821 cggcaaggtt atcgaattta gtgatgattc catcgaagcg cgtcagcgtg aaattgccgc
    17881 aaaacatggc attcgcctga ctaaccacag tctctatctt tacggtcact gtgccgaagg
    17941 cgattgccgc gaagatgagc acgcgcacga aggcaaataa gccagcctga acgagaaaag
    18001 ccaacctgcg ggttggcttt tttatacaag ggaaataaga aaagcgagag ttacagctct
    18061 cacttatttg ttattactgc gtatttcctg ccagacctta tcacaatcgg ctttcaccgc
    18121 ctgatcattt ccggtttgcg ttgcacgcag acactgctga taatccagta cgcgtacact
    18181 ttcctggtcg gcaaactgct gatgttgttt ctctttcttc agcacgttta acacgctctg
    18241 gcaggcttca attttttctg gcgaaccttg cgcggtgttg atacaggcgc tgtatgcctc
    18301 tttcagacgg ctgtcttcct gtggtgcggt tgattgcgca caagctacca gccccgatgc
    18361 catcatggcg atgagaatca gttttttcat tgaatgcggc aggcacaaaa cctgccgcgt
    18421 cgggcatcag aagatggtga atggtgcgat taccataaat ttcacgtcac gctcatcctg
    18481 gaagatgtta ccgtaaccgc cgccccagct tgggatgtcg gagtggttgt cgtattcggt
    18541 gaagtgcagt ttgaacatcg tgcctttggc gcgaccgtcc tgaatggtgt agaccgcatc
    18601 caggctgtag gcagactctt caatagtccg gtttttgtcg tagtacgcat ccggattgct
    18661 ctgccaggtc gcaggtttag catcccatgc gtaaacgtag gaagcgccga tggcgaagcc
    18721 tggaagattc cagtttttca ggtcatacat cgcactgaag aagaccgctt tttcgccgtt
    18781 agcgttgaag tcggaacggt tatcccacca gatatccagg cggccgttgg aggaagcgta
    18841 ggttggggtc atacgttgca ggaagtatcc ctgctgactg tcagccttaa tccaggtgcc
    18901 ttcgaggcgc aaatccacta cgtcagccgc ccggtaacca aaggtcaacg cttgcagcca
    18961 ggcggtgccg tcataaagat cgttgacgct gcgatcgtca actttatcgc gggtaccata
    19021 gaactggtag ctggtggtta acgggctacc ggcgatatca aatttgtagc tggctttggc
    19081 aaaatattga tcgatatacc cttccgcctg accaaatgcc gcttccagta cgaagttatt
    19141 tttgaagtcg tatttcgcac cgatggagtg cagataatca actttggtgg ttttatcgtt
    19201 ctgataaaac tcatccattt ccagatgcca cggcgctttg tattcgttgg tccacatgta
    19261 ggagaaactc aaggcaccag catcgccgta atcaaaattc gccccggctt ccgccccctg
    19321 ataagtaccc ggcataaagc tccagtgcgg cgctaacagc gtttgaccgg ttggctgaat
    19381 gtaacctgcc ctcgcccaaa ccggaccgta tttaaatttg gccgcagctt tatacaggct
    19441 tataccgctt ttgtcgccgg accagtcttc gtcataggct ttattacttt ttgaaaacgc
    19501 gatttcgttc gggtgggagc tgtcgccgtt ttccgccatt tcaatcgccg taaacgcagc
    19561 aatatcaagg ccgaacatat cagcagcata accggactga aaatcgaggt tggcattcca
    19621 ggtggagtga gaaaggttgg ttttgtattt gtcgccgtcg gtaacatctt tacggtcgcg
    19681 ttcacgctgc cagtaataga taccgccggt taaggttgaa tcgtcgatga atccttcggc
    19741 gcgagcctcc ggcattgcca taaagcccga cattgctgta acaccggcaa tagccagcgc
    19801 cagcgtacta cgtttgccac taaacgtacg catgggttaa tcctctttga cgtataaatt
    19861 gctgcaccaa aggtgaacag cgaaaaaaaa taaaaattaa aaaagtagcg ttgagaaaag
    19921 tctcgctgac tacaggaaca gactattttt cgcgacgcga attatcaatc tctttctgta
    19981 accagaatgt aagaacatga gcaaccgcac atattttgat gatttttgtt acaaggctta
    20041 agtttgcggt tgaaaggctt gcagaagaat aaaaacgtga aattccagca agatgaaagc
    20101 ggttacattt tatttaatta tgttttgtga ttttaaattt attttatgga taaattgcga
    20161 agcggaatta attcgcgaag aaaagcagaa aaaacgccgc agagcgcggc gtttgggatt
    20221 gtttgctatt tcttgccgga tgcggcgtga acgccttatc cggcctacaa aagcacgcaa
    20281 attcaatata ttgcagagat catgtaggcc tgataagcga agcgcatcag gcaatttagc
    20341 gtttgaaatt tattgtctat ttattgccgg atgcgacgta agtgcctcac ccggcttggt
    20401 agaacttaaa attactcgcc tactttcgcc caggtatcac gcagcccaac ggtgcggtta
    20461 aataccggtt tttcggccgt ggaatggcgg ctgtcgaggc agaagtaacc ttcacgctca
    20521 aactggaatg ctttacccgc aaccgcatct ttcagcgacg gttcagcaaa gccctgtttg
    20581 atcaccagcg attccgggtt aatcaccgac aggaaatcat ccgcagcacc tgggttaggc
    20641 acgctgaaca gacgatcata caaacggatt tcaaccggca gcgcatgtgc cgcgctcacc
    20701 cagtgaataa cacctttgac tttacgacca tctgccggat ctttgcttaa ggtgtcggcg
    20761 tcataagtac agaagatggt ggtgatatta ccttcggcat ctttctccac gcgttcagcc
    20821 ttaatgacgt aagcattacg cagacgcact tctttaccca gcaccagacg tttgtactgc
    20881 ttgttagctt cttcgcggaa atcggcgcga tcgatccaaa tttcaccgct aaacggcacc
    20941 tgacggctcc ccatttccgg tttgttcgga tggttcggca tggtgaccat ttcgccttcg
    21001 ccctgatagt tttcgataac cagtttcacc ggatcgataa ccgccattgc gcgcggcgca
    21061 ttttcgttga gatcttcacg gatgcaggat tccagcgacg ccatctcaat ggtgttgtcc
    21121 tgcttggtca cgccgatgcg tttgcagaac tcacgaatag aagccgcagt gtaaccacga
    21181 cgacgcagac cggaaatggt cggcatacgc gggtcatccc agccttcaac gtgcttgtcg
    21241 gtcaccagca ggttcagctt acgcttggac atcacagtgt attccagatt caggcgcgag
    21301 aattcgtact ggcgcggatg aacaggaatg gtgatgttat ccagtaccca gtcgtacaga
    21361 cgacggttgt cctggaactc aagcgtacac agagagtgcg taataccttc cagcgcatcg
    21421 ctgatgcaat gggtgaagtc gtacatcggg tagatgcacc acttgttgcc agtctggtgg
    21481 tgttcagcaa atttaatgcg gtacagcacc ggatcgcgca ttacgataaa tggcgacgcc
    21541 atgtcgattt tcgcacgcag gcaggcttta ccttcttcaa aaccaccggc acgcattttt
    21601 tcgaacagcg ccaagttctc ttcaacgctg cggtcgcggt acgggctgtt tttacccggc
    21661 tgcgtcaggg taccgcggta ttcgcggatc tgttccggtg tcagttcatc aacgtacgcc
    21721 aggcctttat tgatcagttc gatcgcatag gcgtggagct gatcaaaata atcggaggag
    21781 taacggacgt taccagacca gtgaaaacct aaccactcta cgtcgttttt gatcgaatcg
    21841 acatactcga tatcttcttt taccgggtta gtgtcgtcga aacgcaggtt gcactggcct
    21901 ttatagtcct gggcgatccc gaagttcagg cagatagatt tcgcatggcc aatatgcaga
    21961 tagccattcg gctccggcgg gaaacgggtg tgtactgtga tgtgcttacc actggccaga
    22021 tcttcatcga tgatctgacg gataaagtta gtcgggcggg cttctgcctc actcatcgtg
    22081 gattcctcaa agcgtaaaca acgtataacg gcgtatgatc ttataagcgg gacaggctga
    22141 caactgttag ttttttaaaa ttgataatct aacaaggatt atggggatga ttgatgaaaa
    22201 aaaaagcggc agagaagatc tccaccgctt gtcattgttg gatgcgacgc tgccgtgtcg
    22261 catcaggcat aaagcagatt actttttgat ttcatacagc ggtgtttgac ccgccacaac
    22321 atggccctga gctttaatga gcaagccact gaaatcgtcg atattgctgc aaaccaccgg
    22381 gctaatcatc gagcgggcgt tagcgttcag gtaatccaga tccatttcca gaatcggttg
    22441 ccctgcgctt acctgcgcac cctcttccac cagacgttta aagcctttac cttccagcgc
    22501 tacggtgtcg atacccatat ggacgacgat ctccgcgcct ttttcggttt ccaggcagaa
    22561 cgcgtggttg gtgttgaaga ttttcacgat tgtccctgcg gctggtgata cgacgatttt
    22621 atctgtcggt ttcaccgcca caccgtcacc caccgctttg ctggcgaatg cttcgtcagg
    22681 aacctgatcc agtgccacga catcaccggt aatcggcgat accagctccg cgatagatac
    22741 cgcgtttggt acagcctgcg gttttgctac aggcgcggca gttgccggag ttgcttcagc
    22801 tgacgcagcg gctaccggac cacgggcaac gactttcttc atcgcatcgc cgatggattc
    22861 tgctttcgcg ccaacaatca cctgaatagt ctgtttgttc agtttcacta caccagaagc
    22921 acccagacgt ttacacatcg tatcgttgac gcgggcagag tcagccacgg tcaggcgcag
    22981 acgggtaata caggcatcaa tcgctttcag gttgtcagta ccgccaaccg cagcaatata
    23041 gttggttgcc agttgagtca gaccttcttc agtattgctg ttggcttctt cagtaacgat
    23101 ctcgtcttct ttatcttcac gacccggcgt tttcaggttg aacatgcgga taaccaaact
    23161 gaacaccacg aagtagatag cgaagaagat aacgcccatc accagcagca tccagacgtt
    23221 ctggctggcg gcaggcaggt tatacatcaa cgcgtagtcg atagcacccg cagagaaaga
    23281 gaagccggca tggataccca gcagcgttgc cacaaacagg ctgataccgg tcagcagtgc
    23341 gtgcaggagg tacagcagcg gcgcaaggaa catgaacagg aattccagcg gctcagtcac
    23401 accggtcagg aacgcagtaa cagcaacaga aagcagcatc ccgccaacca tcggacgacg
    23461 ctctttcggt gctgcgaagt acatcgccag cgccgcaccc ggcagaccga acatcatgat
    23521 cgggaagaag ccggacatga acatccccgc ggtgccgtca ccagcgtaga aacggttgat
    23581 gtcgccgtgg aaaaccgtac ccgccgcgtt ggtgaattca ccaatctgga accaggcgat
    23641 ggtgttcagc acctgatgca gaccggttgg gatcagcaga cggttgatga aaccaaagat
    23701 accggaaccc agcgcgcccg cagaaacgat ccactcgccg cctgcatgga tagcgtgctg
    23761 taccggcggc cagacgtaac caaaaatggc cgccagcacc agacagaaga agccggtggc
    23821 gatcggcaca aagcgtttgc cgccgaagaa gctcaggaag tccggcagtt taatatcgga
    23881 ccaacggtta taggctgcgc caccaaccag accggtaatg atacccgcca gtacacccat
    23941 gttaatttct gggttgatgg tcaccatcgc tttggttaac acaaagtaac ctaccgcacc
    24001 cgccagcgcc gccgcacctg cgctgtcttt cgaccagctg gatgccacac cgatggcgaa
    24061 gattaatgcg aggttatcaa aaattgcacc gcccgcctgg gcaataaacg caacgttaag
    24121 taaatctggc tgaccgaatc gcagcaacag cgccgccacc ggcagcaccg cgatagggag
    24181 ctgtaacgcc ctaccgagtc gctggaaaaa acctaaaata ttcatcttat tccccctacg
    24241 agaaccctat ttggctcgtt taaagccgta tttttatttt gctgcaaatt ttactgccga
    24301 tgttctgtaa tcagattgtt agatcatctg ctacagagtg tgtgaaaatt taattcgcat
    24361 cgcaaattaa acgcgtgtct tttgtgagtt ttgtcaccaa atatcgttat catcactccc
    24421 ttttactggc taaaccagaa aacttatttt atcattcaaa aaatcaagtc ggattgacgc
    24481 ctgtctgcgc aaatccaggt tacgcttaaa gatgtctaat ccgccaacgg cttacatttt
    24541 acttattgag gtgaataatg agactgatcc ccctgactac cgctgaacag gtcggcaaat
    24601 gggctgctcg ccatatcgtc aatcgtatca atgcgttcaa accaactgcc gatcgtccgt
    24661 ttgtactggg cctgccgact ggcggcacgc cgatgaccac ctataaagcg ttagtcgaaa
    24721 tgcataaagc aggccaggtc agctttaagc acgttgtcac cttcaacatg gacgaatatg
    24781 tcggtctgcc gaaagagcat ccggaaagct actacagctt tatgcaccgt aatttcttcg
    24841 atcacgttga tattccagca gaaaacatca accttctcaa cggcaatgcc ccggatatcg
    24901 atgccgagtg ccgccagtat gaagaaaaaa tccgttctta cggaaaaatt catctgttta
    24961 tgggcggtgt aggtaacgac ggtcatattg catttaacga accggcgtct tctctggctt
    25021 ctcgtactcg tatcaaaacc ctgactcatg acactcgcgt cgcgaactct cgtttctttg
    25081 ataacgatgt caatcaggtg ccaaaatatg ccctgactgt cggtgttggt acactgctgg
    25141 atgccgaaga agtgatgatt atggtgctgg gtagccagaa agcactggcg ctgcaggccg
    25201 ccgttgaagg ttgcgtgaac catatgtgga ccatcagctg tctgcaactg catccgaaag
    25261 cgatcatggt gtgcgatgaa ccttccacca tggagctgaa agttaagact ttaagatatt
    25321 tcaatgaatt agaagcagaa aatatcaaag gtctgtaatt gttatccctg ccctggttcc
    25381 ttgctcaggg caatattttt taaaatcggg gggcagaatg tatgcattaa cccagggccg
    25441 gatctttacc ggccacgaat ttcttgatga ccacgcggtt gttatcgctg atggcctgat
    25501 taaaagcgtc tgtccggtag cggaactgcc gccagagatc gaacaacgtt cactgaacgg
    25561 ggccattctc tcccccggtt ttatcgatgt gcagttaaac ggctgcggcg gcgtacagtt
    25621 taacgacacc gctgaagcgg tcagcgtgga aacgctggaa atcatgcaga aagccaatga
    25681 gaaatcaggc tgtactaact atctgccgac gcttatcacc accagcgatg agctgatgaa
    25741 acagggcgtg cgcgttatgc gtgagtacct ggcaaaacat ccgaatcagg cgttaggtct
    25801 gcatctggaa ggtccgtggc tgaatctggt aaaaaaaggc acccataatc cgaattttgt
    25861 gcgtaagcct gatgccgcgc tggtcgattt cctgtgtgaa aacgccgacg tcattaccaa
    25921 agtgaccctg gcaccggaaa tggttcctgc ggaagtcatc agcaaactgg caaatgccgg
    25981 gattgtggtt tctgccggtc actccaacgc gacgttgaaa gaagcaaaag ccggtttccg
    26041 cgcggggatt acctttgcca cccatctgta caacgcgatg ccgtatatta ccggtcgtga
    26101 accgggcctg gcgggcgcga tcctcgacga agctgacatt tattgcggta ttatcgctga
    26161 tggcctgcat gttgattacg ccaacattcg taacgctaaa cgccttaaag gcgacaaact
    26221 gtgtctggtt accgacgcca ccgcgccagc aggtgccaac attgaacagt tcatttttgc
    26281 gggtaaaaca atatactacc gtaacggact ttgtgtggat gagaacggta cgttaagcgg
    26341 ttcatcctta accatgattg aaggcgtgcg taatctggtc gaacattgcg gtatcgcact
    26401 ggatgaagtg ctgcgtatgg cgacgctcta tccggcgcgt gcgattgacg ttgaaaaacg
    26461 tctcggcacg ctcgccgcag gtaaagtagc caacctgacc gcattcacac ctgattttaa
    26521 aatcaccaag accatcgtta acggtaacga ggtcgtaact caataagaga aagtatgaca
    26581 ccaggcggac aagctcagat aggtaatgtt gatctcgtaa aacagcttaa cagcgcggcg
    26641 gtttatcgcc tgattgacca gtacgggcca atctcgcgga ttcagattgc cgagcaaagc
    26701 cagcttgccc ccgccagcgt aaccaaaatt acgcgtcagc ttatcgaacg cgggctgatc
    26761 aaagaagttg atcagcaggc ctccaccggg ggccgccgcg ctatctccat cgtcaccgaa
    26821 acccgcaatt tccacgcaat cggcgtacgg cttggtcgtc atgacgccac catcactctg
    26881 tttgatctca gcagcaaagt gctggcagaa gaacattacc cgctgccgga acgtacccag
    26941 cagacgctgg aacatgccct gctgaatgcc attgctcagt ttattgatag ctaccagcgc
    27001 aagctgcgcg agctaatcgc catttcggtg atcctgccag ggcttgttga cccggacagc
    27061 ggcaaaattc attacatgcc gcatattcag gtagaaaact gggggctggt agaagcactg
    27121 gaagaacgtt ttaaagtgac ctgtttcgtt ggtcacgata tccgtagtct ggcgctggcg
    27181 gagcactact tcggtgcaag tcaggattgc gaagactcca ttctggtgcg tgtccatcgc
    27241 ggaaccgggg ccgggattat ctctaacggg cgcattttta ttggccgcaa cggtaacgtc
    27301 ggtgaaattg gccatattca ggtcgaaccg ctgggtgaac gctgccactg cggcaacttt
    27361 ggctgcctgg aaactatcgc cgccaacgct gccattgaac aacgggtgtt gaatctgtta
    27421 aagcagggct accagagccg tgtgccactg gacgactgca ccatcaaaac tatctgcaaa
    27481 gccgcgaaca aaggcgatag cctggcctcg gaagtgattg agtatgtcgg tcgtcatctg
    27541 ggtaaaacca tcgccattgc tatcaactta tttaatccgc aaaaaattgt tattgctggt
    27601 gaaatcaccg aagccgataa agtgctgctc cctgctattg aaagctgcat taatacccaa
    27661 gcgctgaagg cgtttcgtac taatctgccg gtggtacgtt ctgagctgga tcaccgctcg
    27721 gcaatcggcg cttttgcgct ggtaaaacgc gccatgctca acggtatttt gctccagcat
    27781 ttgctggaaa attaatgtgc ttttatagtg gcgcttattg ttgtcaatat tctgggtagt
    27841 ccatgaccat taaaaatgta atttgcgata tcgacggcgt gctgatgcac gataacgtcg
    27901 ccgtaccggg tgcagcggaa tttttgcacg ggattatgga taaaggcctg ccgctggtgt
    27961 tgctgaccaa ctatccttcg cagactgggc aagatctggc gaaccgcttt gccactgccg
    28021 gtgtcgatgt gccggatagc gtgttttata cctctgcgat ggcgaccgcc gatttcctgc
    28081 gtcgctagga aggcaagaaa gcgtatgtgg tgggcgaagg cgcactgatt catgaactgt
    28141 acaaagccgg tttcactatt accgatgtga accctgattt cgtgattgtt ggcgaaacac
    28201 gttcctacaa ctgggacatg attcataaag cagcctattt cgtcgctaac ggtgcacgct
    28261 ttatcgccac caacccggac acccacgggc gcggttttta tcccgcttgt ggcgcgttgt
    28321 gtgcagggat tgagaaaatc tccgggcgca aaccgttcta tgttggtaag cccagcccgt
    28381 ggatcatccg cgcggcatta aacaaaatgc aggcgcattc ggaagaaaca gtgattgtcg
    28441 gcgataacct gcgtaccgat attctggcgg gcttccaggc cggtctggag acgattctgg
    28501 tgctttctgg tgtttcatcg ctcgacgata tcgacagtat gccgttccgc ccaagctgga
    28561 tttacccgtc agtcgctgaa atcgacgtta tctgaatacc acccacgtct gcggacgtgg
    28621 ttggctctga tgacaaacgc caaactgcct gatgcgctac gcttatcagg cctacgcagc
    28681 tcctgcaata tattgaattt tcatgctttt gtaggccgga taaggcgttc acgccgcatc
    28741 cggcatgaac aaatcgcact ttatcagcaa tttggccccc gtaaaatata accattcaat
    28801 aaaacccgct aaaaattatc gattcatcaa taaatacatc ctcacaccag accctttttc
    28861 accaatcgac aatcaccatt acgtttttta tttttgcact gccaaatagc ttgcgcacag
    28921 tgctgctttg cggcattttt ttaaacaagc aaacacaaca agcaacaaat accaggttaa
    28981 cggagaaggt tatgtgttca atttttggcg tattcgatat caaaacagac gcagttgagc
    29041 tgcgtaagaa agcactcgag ctgtcacgcc tgatgcgtca tcgtggcccg gactggtccg
    29101 gtatttatgc cagcgataac gccattctcg ctcacgaacg tctgtcaatt gttgacgtta
    29161 acgcgggggc gcaacctctc tacaaccaac aaaaaaacca cgtactggcg gtaaacggtg
    29221 aaatctacaa ccaccaggca ttgcgcgctg aatatggcga tcgttatcag ttccagacag
    29281 ggtctgactg tgaagtgatc ctcgcgctgt atcaggaaaa agggccggaa tttctcgacg
    29341 acttgcaggg catgtttgcc tttgccctgt acgacagcga aaaagatgcc tacctgattg
    29401 gtcgcgacca tctggggatc atcccactgt atatggggta tgacgaacac ggtcagctgt
    29461 atgtggcctc agaaatgaaa tcgctggtgc cagtttgccg cacgattaaa gagttcccgg
    29521 cggggagcta tttgtggagt caggacggcg aaatccgttc ttattatcat cgcgactggt
    29581 tcgactttga tgcagtgaaa gataacgtga ccgacaaaaa cgagctgcgt caggcactgg
    29641 aagattccgt taaaagccat ctgatgtctg atgtgcctta cggtgtgcta ctttctggtg
    29701 gtctggattc ctcaattatt tccgctatca ccaagaaata cgcagcccgt cgcgtggaag
    29761 atcaggaacg ctctgaagcc tggtggccgc agttacactc ctttgctgta ggtctgccgg
    29821 gttcaccgga tcttaaagca gcccaggaag tggcaaacca tctgggcacg gtgcatcacg
    29881 aaattcactt cactgtacag gaaggtctgg atgccatccg cgacgtgatt taccacatcg
    29941 aaacttatga tgtgaccact attcgcgctt caacaccgat gtatttaatg tcgcgtaaga
    30001 tcaaggcgat gggcattaaa atggtgctgt ccggtgaagg ttctgatgaa gtgtttggcg
    30061 gttatcttta cttccataaa gcgcccaacg ttaaagaact gcatgaagag acggtgcgta
    30121 aactgctggc cttgcatatg tatgactgcg cgcgcgccaa caaagcgatg tcagcctggg
    30181 gcgtggaagc acgcgttccg ttcctcgaca aaaaattcct tgatgtggcg atgcgcatta
    30241 acccgcagga taaaatgtgc ggtaacggca aaatggaaaa acacatcctg cgtgaatgtt
    30301 ttgagtcata cctgcccgca agcgtggcct ggcggcagaa agagcagttc tccgatggcg
    30361 tcggttacag ttggatcgac accctgaaag aagtggcggc gcagcaggtt tctgatcagc
    30421 aactggaaac tgcccgcttc cgcttcccgt acaacacgcc gacctcaaaa gaagcgtatc
    30481 tgtaccgcga gatctttgaa gaactgttcc cacttccgag tgccgctgag tgcgtgccgg
    30541 gcggtccttc cgtcgcgtgt tcttcagcta aagcgatcga atgggatgaa gcgttcaaaa
    30601 aaatggacga tccgtctggt cgtgcggttg gcgttcacca gtcggcgtat aagtaagaaa
    30661 tgcggtactg ccccggagaa atccggggct gttttagtta ttgcgctgct tgttgattct
    30721 gatatgcagt aaggaaatgt tccgggtttg ccacatcact gtttgacaac tcatcactgt
    30781 aggccagtat ttcatagatt aatttcccac cactattgtt gaattccgaa tttcatcctg
    30841 tacgacaaaa gaatggctgt agggtgggga aaaacacaaa gtaaacaata attgacgaat
    30901 atagcgccac gctgttcgca acctaaccaa acagtcactt tcgagcaatt ttccttgaaa
    30961 aagaggttga cgctgcaagg ctctatacgc ataatgcgcc ccgcaacgcc gataaggtat
    31021 cgcgaaaaaa agatggctac gtagctcagt tggttagagc acatcactca taatgatggg
    31081 gtcacaggtt cgaatcccgt cgtagccacc atcttttttg cgggagtggc gaaattggta
    31141 gacgcaccag atttaggttc tggcgccgca aggtgtgcga gttcaagtct cgcctcccgc
    31201 accattcacc agaaagcgtt gtacggatgg ggtatcgcca agcggtaagg caccggtttt
    31261 tgataccggc attccctggt tcgaatccag gtaccccagc catcttcttc gagtaagcgg
    31321 ttcaccgccc ggttattggg gtatcgccaa gcggtaaggc accggttttt gataccggca
    31381 ttccctggtt cgaatccagg taccccagcc atcgaagaaa taatctggct acgtagctca
    31441 gttggttaga gcacatcact cataatgatg gggtcacagg ttcgaatccc gtcgtagcca
    31501 ccaaattctg aatgtgtcga atacgttcgg caaattcaaa aaccaatttg ttggggtatc
    31561 gccaagcggt aaggcaccgg attctgattc cggcattccg aggttcgaat cctcgtaccc
    31621 cagccaattt attcaagacg cttacatcgt aagtgcaccc agttggggta tcgccaagcg
    31681 gtaaggcacc ggattctgat tccggcattc cgaggttcga atcctcgtac cccagccaca
    31741 ttaaaaaagc tcgcttcggc gagctttttg cttttctgcg ttcattcaat gtcgaatgcg
    31801 atgttgacac gtcttatcct tcaatgtcgg atgcgacgct gccgcgtctt atccgaccta
    31861 cggctggcac gcatccggca atgttgtaag gctacaaccc taacgcatat ttcagcgcct
    31921 gacgtttcaa cacgccagca cgctctgccg ccattaaccc gagattacgc acaaaacgca
    31981 gcggcggcag attattactg aatccggcat aaaacagatc cataccactt tgcataatga
    32041 agttatccgc catgcgccgc atctggtaac gcttgaggac aggataactg gcccacgctt
    32101 caccgtagct gcgggcattc accagaacat caatcagggc atcgacatca cgataaccaa
    32161 gattcacccc ctgccccgcc agcggatgga tggtatgcgc ggcatcgccc accagcgcaa
    32221 gccccggctg cacatactgc aacgcatgtc ggcgcgtcag cggaaacgca ccagcggcaa
    32281 gcggtgtaac gtaacccaga cgcgacggga aatgcttcgc gatttccgcc tggagttgcg
    32341 ccatattcat attctgcaac tggcgaatac gcgtcggaga gtcataccac accagcgatg
    32401 cccagttatc aaacaacggc agaaacgcac gcggtccgtc cggggtaaat tgctgccagg
    32461 tgctgtcgcc gggatcgttc tcacactgta cgctaatcaa catgcacgac tgcgcatact
    32521 gccaggcatg aacgccaatt cccgccatct gccgcacctg agaatttgcg ccgtcggcac
    32581 caatcaccag tttcgcgcgg atcgtttcac cgcccttcag ttccagctcc tgaagatcat
    32641 tatctcgatg taatgcaatc agcgaggttg gcacacgtaa cgttactttc ggatgcgctt
    32701 ccagcgcctg ccacaacgcc tgttgcagga ctgtgttttc caccatgtag ccaagcaacg
    32761 gcaactttaa ttcggctgcg tcaaatacca catgtgccgt ttcccactcc caggtttcca
    32821 gtctgcggta aggatggcaa cgcatagcct gtactgcatc ccagacccct aaccctttaa
    32881 gcaatgatac cgaagccgcg ctgatcgccg agatccgcac gtccggttgg ctgtcagcga
    32941 caaacggtgc aggttctgcg tgctcgatca ccgttaccga aaatccgtgc tgtgccagcc
    33001 ccagcgccag tgcgccgccg accattcctc cgccgacaat ggcaatttcc gttggttgat
    33061 ttgtcatggt caatcatcct gttagcaata tgctttaagt ttacaggatt tttaccccca
    33121 agggctgata acgctcatac tggtcacaac ggcagcaaag cattacacta tgcgccctgc
    33181 attcctggct actatttcgc aagagcaagt cgatgaccaa aaaactccat attaaaacct
    33241 ggggctgtca gatgaacgag tacgattcat cgaagatggc cgatctgctg gatgccaccc
    33301 acggctatca actgaccgac gtggcggaag aagcggatgt gctactgctg aacacctgct
    33361 caatccgcga gaaggctcag gaaaaagtct tccatcagtt gggtcgctgg aaactgttaa
    33421 aagagaagaa tccagacctg attatcggcg tcggtggctg cgtggcatcg caagaaggcg
    33481 agcacattcg ccagcgcgcc cactatgtcg atattatttt tgggccgcaa acgctgcacc
    33541 gtctgccgga gatgatcaac tccgtgcgcg gcgaccgcag cccggttgta gatatcagct
    33601 tcccggaaat cgagaagttt gaccgtctgc cggaaccgcg cgccgaaggg ccgaccgcgt
    33661 ttgtctccat catggaaggc tgcaataaat attgcaccta ctgcgtggtg ccttacaccc
    33721 gtggtgaaga ggtaagccgt ccgtctgacg atattctgtt tgagattgcc cagcttgcgg
    33781 ctcagggcgt gcgtgaagtc aacctgctcg gtcagaatgt gaatgcctgg cgtggtgaga
    33841 actacgacgg caccaccgga tcgtttgccg atctgttgcg tctggttgct gcgatcgacg
    33901 ggatcgatcg tattcgcttt accaccagcc atccgatcga attcaccgac gatatcatcg
    33961 aagtgtaccg cgacacgcct gagctggtga gcttcctgca tctgccggta cagagcggtt
    34021 ccgatcgcat tctgaacctg atgggacgta cccatacggc gctggagtac aaagcgatca
    34081 tccgtaaact gcgtgcggcg cgtccggata ttcagatcag ttctgacttc attgttggct
    34141 tccctggcga aaccaccgaa gacttcgaga aaacgatgaa gctgattgcc gacgtcaatt
    34201 tcgacatgag ctacagcttt atcttctctg cacgtccagg aacgcctgcc gccgatatgg
    34261 ttgatgatgt tccggaagaa gagaagaagc agcgtctgta tattctgcaa gaacgtatca
    34321 atcagcaagc gatggcatgg agccgtcgga tgctcggcac cacccagcgt attctggtag
    34381 aagggacttc gcgtaagagc atcatggagc tttccgggcg taccgaaaat aaccgcgtgg
    34441 tcaacttcga gggcacaccg gatatgatcg gtaaattcgt cgatgtagaa attaccgacg
    34501 tctacccgaa ctctctgcgc ggtaaagtgg tgcgaactga agatgaaatg ggtctgcgca
    34561 tggcagaaac accggagtcg gtgattgccc gcacccgcaa agaaaacgac cttggcgtgg
    34621 gttattatca gccgtaattc tcaggcctgc cgttccggtg ggccttgttt tttctctctt
    34681 tatccccatc tttatcgcaa tgcttgcgcc tttttcctgt ggcgtgaata atttccttat
    34741 aggccggtga atgttcagct tccggcggca tacagataag aggaacggtt tgaacataga
    34801 cactcgcgaa atcaccctgg agccagcaga caatgcgcgt ctgttgagcc tgtgcggccc
    34861 gtttgatgac aacatcaagc agctcgaacg ccgtctcggc atcgagatca atcgccgcga
    34921 taaccacttt aaactgaccg gccgtccgat ttgcgtcacc gctgcggcag acattctgcg
    34981 tagcctgtat gtcgatactg ccccgatgcg cggtcagatt caggatatcg aaccggaaca
    35041 gatccacctt gcgattaaag aagcgcgggt actggagcaa agcgcggaga gcgtgccgga
    35101 gtacggcaaa gcggtcaata tcaaaactaa acgcggcgta attaagccgc gcacgccaaa
    35161 ccaggcgcag tacatcgcca atattctcga ccatgacatt accttcggcg ttggcccggc
    35221 gggtacgggt aaaacctacc tggcagtggc tgcggcagtt gatgccctgg agcgtcagga
    35281 aattcgccgt attctgctga ctcgtccggc ggtcgaagcc ggtgagaaac tgggcttcct
    35341 gcctggcgat ttaagccaga aagtagaccc gtatctgcgc ccactgtacg acgcgctgtt
    35401 tgaaatgctg ggctttgaga aagtcgagaa actgattgag cgcaacgtta ttgaagtcgc
    35461 gccgctggcc tatatgcgtg gtcgtacgct gaacgacgcg tttatcattc tcgatgagag
    35521 ccagaacact accatcgaac agatgaagat gttcctgacc cgtatcggtt ttaactcaaa
    35581 agcggttatc accggcgacg tcacacagat cgacctgccg cgtaatacta aatcaggctt
    35641 acgtcatgcc attgaagtgc tggccgatgt cgaagagatc agctttaact tcttccacag
    35701 cgaagacgtg gttcgtcacc cggtggtggc gcgtatcgtt aacgcttatg aagcctggga
    35761 agaagcagaa caaaaacgta aagcggcgct ggcagcagaa cgcaagcgcg aagaacagga
    35821 acaaaaatga gtcaggtgat cctcgattta caactggcat gtgaagataa ttccgggtta
    35881 ccggaagaga gccagtttca aacatggctg aatgcggtga tcccgcagtt tcaggaagag
    35941 tcggaagtga ctattcgcgt ggtcgatacc gccgaaagcc acagtctgaa tctgacctat
    36001 cgcggtaagg ataagccgac caacgtgctc tccttcccgt ttgaagtgcc gcctggcatg
    36061 gagatgtcgc tactgggcga tctggttatc tgccgtcagg tggttgagaa ggaagctcag
    36121 gagcaaggca aaccactgga ggcgcactgg gcgcatatgg tggtgcacgg cagtctgcat
    36181 ttgttaggtt acgatcacat tgaagatgac gaagcagaag aaatggaagc cctcgaaaca
    36241 gagattatgc ttgctctggg ctatgaggat ccgtacattg ccgagaaaga ataatttgcc
    36301 aggcatttga ctggcagacg tccccgccgt tggtgattaa cgacggcgtt ttgattaact
    36361 aacatgagaa cccatacgac gccatgagcg acgacaattc acacagtagt gacacgataa
    36421 gcaacaagaa gggatttttc tccctgttac tcagccaact tttccacggt gaaccgaaaa
    36481 accgtgacga actgctggcg ctgatccgtg attccgggca gaacgacctt atcgacgaag
    36541 atacgcgcga tatgctcgaa ggggtgatgg acatcgcaga ccaacgcgtt cgcgacatca
    36601 tgatcccccg ctcccagatg attaccctga aacgcaacca gacgctggac gaatgccttg
    36661 atgtcatcat cgagtccgcc cactcacgtt tcccggtgat tagcgaagac aaagatcaca
    36721 ttgaagggat tctgatggcg aaagacttgc tgccgtttat gcgcagcgat gctgaagcct
    36781 tcagcatgga caaagtgtta cgtcaggcgg ttgtcgttcc tgaaagtaag cgcgtagacc
    36841 ggatgctgaa agagtttcgc tctcagcgtt accacatggc gatcgttatt gacgaattcg
    36901 gtggggtttc cggtctggtg accattgaag acatcctgga actgattgtt ggtgagattg
    36961 aagacgagta tgacgaagaa gatgatatcg acttccgtca gctgagtcgt catacctgga
    37021 ccgtgcgcgc actggcttcc attgaagact tcaacgaagc gttcggcacc cactttagcg
    37081 atgaagaagt cgacactatc ggtggtctgg tgatgcaggc gtttgggcat cttccggcgc
    37141 gtggcgaaac tatcgacatc gacggttacc agttcaaagt ggcgatggcc gatagtcggc
    37201 gtattattca ggttcatgtc aaaatcccgg atgactcacc ccagccaaag ctggatgaat
    37261 aaaaccgaaa ctggatagat aactacatgg attttgcctc attaattgaa cgccagcgca
    37321 ttcgcctgct gctggcgtta ttattcggtg cctgcggaac gctggccttc tctccttacg
    37381 acgtctggcc tgcggcgatt atttcgctga tggggcttca ggcgttgacc tttaaccgtc
    37441 gcccactcca gtctgccgct attggctttt gctggggatt tggcctcttt ggcagcggta
    37501 ttaactgggt ctatgtcagc atcgcgacct ttggcgggat gcctggcccg gttaacatct
    37561 tcctggtggt gctgctggcg gcgtatttgt cgctgtatac cggactgttt gctggcgtgc
    37621 tgtcgcgtct gtggccgaaa accacctggc tgcgcgtggc gattgccacc cctgctctct
    37681 ggcaagtgac cgagtttctg cgcggttggg tgctgactgg cttcccgtgg ttacagttcg
    37741 gctatagcca gattgatggc ccgttaaaag ggctggcacc gctaatgggc gtggaagcca
    37801 ttaacttcct gctgatgatg gttagtggcc tgctggcact ggcgttggtc aaacgcaact
    37861 ggcgtccgct ggtggtggcc gtcgtgctgt ttgccctacc cttcccgctg cgttacatcc
    37921 aatggtttac cccgcaaccg gagaaaacca ttcaggtttc gatggttcag ggcgatattc
    37981 cgcaatcgct gaaatgggac gaaggccagc ttcttaatac gctaaagatt tactacaacg
    38041 caacggcacc gctgatgggc aaatcatcgt tgattatctg gccggagtcg gcgataaccg
    38101 atctggaaat taatcagcaa ccgttcctca aagcactgga cggtgagttg cgtgataaag
    38161 gtagctcgct ggtaaccggg attgtcgacg cgcgtctcaa taagcagaac cgctacgata
    38221 cctacaacac catcatcacg ctgggtaaag gtgcaccgta cagctacgaa tcagccgatc
    38281 gctataacaa aaaccatctg gtgccgtttg gcgagtttgt cccgctggag tcgattctgc
    38341 gtccgttagc accgttcttt gatctgccga tgtcgtcgtt cagccgtggg ccatatatcc
    38401 agccgccgct gtcggcaaat ggtattgagc ttactgcggc tatttgctac gagattattc
    38461 tcgccgagca agtgcgcgat aacttccgcc cggataccga ctacctgctg actatctcca
    38521 acgatgcgtg gtttggtaaa tctattggtc catggcaaca cttccagatg gcgcgaatgc
    38581 gtgcgctgga gctggcgcgc ccactgttgc gcagcaccaa caacggcatt acggcggtga
    38641 ttggcccgca gggtgagatt caggcgatga tcccgcagtt cgcccgcgag gtgttaacca
    38701 ctaacgtgac gccgaccacc ggactcacac catacgcacg taccggcaac tggccgctgt
    38761 gggtgctgac ggcactgttt ggttttgctg ctgtgttgat gagtctgcgt cagcgacgta
    38821 aataatccct tcttatcgtg ccggaataca tcccatattt cggcacgctc accggatgcg
    38881 gcatcagtgc attatccgga taacaatatt ctcctatcca actttggcac atctattgct
    38941 ttgttataca aggcaaagct tgaaccagca tcagtacaac ccggttgcac cacagcaatc
    39001 caccggtagc accaaaagag tgcattttaa gaattttgct tcagaatggt gcggtgcacc
    39061 tcgcaaaaac aaacaataaa acatcaatat ctttacattt agccaaacta aatgttacct
    39121 aacaggcaca acactgcaca ataaagttgc agacgataac aacacaaaca ctcacaacgg
    39181 gtatccatgc gttcttaacg cagaagataa aggtgttgga tatgcaatta cgtaaacctg
    39241 ccacagcaat cctcgccctg gcgctttccg caggactggc acaggcagat gacgccgccc
    39301 cggcagcagg cagtacgctg gacaaaatcg ccaaaaacgg tgtgattgtc gtcggtcacc
    39361 gtgaatcttc agtacctttc tcttattacg acaatcagca aaaagtggtg ggttactcgc
    39421 aggattactc caacgccatt gttgaagcag tgaaaaagaa actcaacaaa ccggacttgc
    39481 aggtaaaact gattccgatt acctcacaaa atcgtattcc actgctgcaa aacggcactt
    39541 tcgattttga atgtggttct accaccaaca acgtcgaacg ccaaaaacag gcggctttct
    39601 ctgacactat tttcgtggtc ggtacgcgcc tgttgaccaa aaagggcggc gatatcaaag
    39661 attttgccga cctgaaaggc aaagccgtag tcgtcacttc cggcactacc tctgaagttt
    39721 tgctcaacaa actgaatgaa gagcaaaaaa tgaatatgcg catcatcagc gccaaagatc
    39781 acggtgactc tttccgcacc ctggaaagcg gtcgtgccgt tgcctttatg atggatgacg
    39841 ctctgctggc cggtgaacgt gcgaaagcga agaaaccaga caactgggat atcgtcggca
    39901 agccgcagtc tcaggaagcc tacggttgca tgttgcgtaa agacgatccg cagttcaaaa
    39961 agctgatgga tgacactatc gctcaggtgc agacctccgg tgaagcggaa aaatggtttg
    40021 ataaatggtt caaaaatcca attccgccga aaaacctgaa catgaatttc gaactgtcag
    40081 acgaaatgaa agcactgttc aaagaaccga atgacaaggc actgaactaa ttacaagaac
    40141 caggggcgga aattccagcc ctctcgattg ttacgtagca cggacagact atacgcctga
    40201 tggtcgttcc ccatcgggcc tgaaaaccgc aatacgctgg gtaacaatct tcgagggtag
    40261 cagttaacgc tgctaccctc ttttttctgg agtagattta tgtctataga ctggaactgg
    40321 ggtatttttt tacaacaagc cccgttcggc aataccacct atcttggttg ggtctggagt
    40381 ggttttcagg tgacgatcgc tttgtcgatc tgcgcctgga ttatcgcttt cctcgtcggt
    40441 tcatttttcg gcattttacg taccgttcca aaccgttttc tctccggtct gggtacgttg
    40501 tatgtcgaac tgttccgcaa cgtgccgttg attgtgcaat tctttacctg gtatctggtg
    40561 atcccggagc tgctgccgga gaaaatcggc atgtggttta aggccgagct ggatcccaat
    40621 attcagtttt tcctttcatc catgctctgc ctggggctgt ttaccgccgc ccgtgtttgc
    40681 gaacaggtac gcgccgctat tcagtcgcta ccgcgtgggc agaagaatgc cgcgctggcg
    40741 atgggcctga cgctaccgca gacttatcga tacgtactgc tgcctaatgc ttatcgcgtt
    40801 atcgtcccgc cgatgacttc cgagatgatg aacctggtga aaaactccgc catcgcctcg
    40861 accatcggtc tggtggatat ggcggcgcag gcgggtaaat tgctcgacta ctcggctcac
    40921 gcctgggagt cgtttaccgc catcacgctt gcgtatgtgt tgattaacgc ttttatcatg
    40981 ctggtgatga cgctggttga acgtaaagtt cgcctgcccg gcaacatggg gggcaaataa
    41041 tgtacgagtt tgactggagt tccattgttc cttccctgcc atatctgctc gacgggctgg
    41101 tgatcaccct gaaaatcacc gtcacggcag tcgtgattgg tattttgtgg ggtaccatgc
    41161 tggcggtgat gcgcttgtcc agcttcgcgc ctgtttcgtg gtttgccaaa gcctacgtta
    41221 acgtattccg ctcgattcct ttagtcatgg ttttgctgtg gttttacttg attgtgccgg
    41281 gatttctgca aaatgtgctg ggattatcgc caaaaaatga tattcgttta atttcggcga
    41341 tggtggcgtt ttcgatgttt gaagcggcct actattcaga gattatccgt gccggtattc
    41401 aaagtatttc ccgtggtcag tcgagcgccg cgctggcgtt ggggatgact cactggcagt
    41461 cgatgaaact gattattctg ccgcaggctt tccgcgcgat ggtgccgctg ctgctcactc
    41521 agggcatcgt actgttccag gatacctcgc tggtgtatgt gttatctctg gccgatttct
    41581 tccgtaccgc ctcaaccatt ggtgagcgtg atggtacgca ggtcgagatg atcctgtttg
    41641 ccggatttgt ttatttcgtt attagcctta gcgcgtcgtt gttggtcagc tacttgaaaa
    41701 gaaggacagc ataatgatta ccctgaaaaa tgtttcaaaa tggtatggtc actttcaggt
    41761 gctgaccgac tgctcaaccg aagtgaaaaa aggcgaagtg gtggtggttt gcggcccgtc
    41821 tggttccggc aaatcaacgc tgattaaaac cgtcaacggc ctcgaaccag tgcagcaagg
    41881 tgaaatcacc gtcgatggta tcgtggttaa cgacaagaaa accgatctgg caaagctgcg
    41941 ctcccgcgtc gggatggtat tccagcattt cgagctgttc cctcatctgt cgattatcga
    42001 aaacctgacc ctggcgcagg tgaaagtgct taaacgcgat aaagcgcccg ctcgtgaaaa
    42061 agcattgaaa ttgctggagc gcgtgggact ttccgcacat gccaataaat ttccggcgca
    42121 gctttccggc ggtcagcagc agcgcgtggc aatcgctcgc gcattgtgta tggctcctat
    42181 tgcgatgctg ttcgacgaac cgacatcggc gctggatccg gagatgatca acgaagtgct
    42241 ggacgtgatg gtggaactgg cgaacgaagg aatgaccatg atggtggtga cccacgaaat
    42301 gggctttgcc cgcaaagtgg cgaatcgggt gatctttatg gacgagggta aaattgtcga
    42361 agactcgccg aaagacgctt tcttcgatga tccgaaatcg gaccgcgcaa aagacttcct
    42421 cgcgaaaatc ctgcattaat cactctggcg cacataccca attgcgcgcc atctcacgct
    42481 ttatcgtttc gcccctcgta tctgttacat cctggcgtta cccttgtcgc aaagaagcac
    42541 acaacaagga gcaacaatgg cactgccaat tctgttagat tgcgacccag gtcatgatga
    42601 cgctatcgca atagttctcg cactcgcctc accagagctt gatgtcaaag caattacgtc
    42661 ttccgccgga aaccagacac cagaaaaaac cttacgcaat gttctgcgta tgctgacctt
    42721 gcttaatcgc accgatattc cggtggcagg cggcgcggta aaaccgttaa tgcgtgagtt
    42781 gattatcgcg gacaatgtgc acggcgaaag cggtctcgac ggcccggcat taccggaacc
    42841 gacgttcgca ccgcaaaact gtacggcggt ggagctgatg gcgaaaacgc tgcgcgaaag
    42901 tgcggaacct gtcacgattg tttctaccgg accgcaaact aacgttgcct tgctgctcaa
    42961 tagccatccg gaactgcata gcaaaattgc ccgtatcgtg attatgggcg gcgcaatggg
    43021 gcttggtaac tggacgcctg cggctgaatt taatatttac gttgacccgg aagcggcaga
    43081 aattgtcttc cagtcaggga tcccggtggt gatggccggt ctggatgtta ctcataaagc
    43141 gcaaatccac gttgaagaca ccgagcgttt ccgcgcgatt ggcaaccctg tttcaaccat
    43201 tgttgccgaa ctgctggatt tcttcctcga atatcataaa gacgaaaaat ggggctttgt
    43261 cggcgcaccg ctgcatgacc catgcaccat cgcctggctg ttgaaacccg agttgtttac
    43321 cactgttgag cgctgggttg gcgtggaaac acaggggaaa tatacccagg gtatgacggt
    43381 tgttgattat tattatctga ctggcaataa accgaatgcc actgtaattg tcgatgttga
    43441 tcgtcagggc tttgttgatt tactggctga tcgtctgaaa ttttacgctt aacactcgtc
    43501 attgattaag atcaccacgc gttacctggg tggtcttaat ctatttatct ctttttcttt
    43561 caagatataa atgcaatgga taatgcagaa ctcgccatag gtatcgatct cggcactacc
    43621 aatagtttaa ttgccgtctg gaaagacggt gccgcgcaat taattccaaa taagttcggt
    43681 gaatatttaa caccatccat aattagcatg gatgaaaata atcatatttt agtcggaaaa
    43741 ccggctgtat cacggcgtac ttcgcatccg gataaaacgg cagcgttatt taaacgtgca
    43801 atgggcagta ataccaactg gcggttaggc agcgacacat ttaacgcgcc agaactgtcc
    43861 tctttggtat tacgctcatt aaaagaagat gccgaagaat ttctgcaacg tccgattaaa
    43921 gatgtggtga tctccgttct ggcttatttc agcgatgaac aacgcaagca tacccgttta
    43981 gcagcggact tagccgggtt aaatgcggta cgcttaatta atgaacccac agcagctgcg
    44041 atggcgtatg gcctgcatac ccaacaaaat acccgttcgc tggtgtttga tctcggtggc
    44101 ggcacgtttg acgttacggt gcttgagtac gccacgccgg tgattgaagt tcacgcctcc
    44161 gctggcgaca acttcctcgg tggcgaagat tttacccata tgttggtcga tgacgtttta
    44221 aagcgcgcgg atgtcacaag gaccacgctt aacgagagtg aactggcagc cttgtacgcc
    44281 tgtgtggaag cggcaaaatg tagcaatcaa tcgccaatgc acattcgctg gtagtaccag
    44341 gatgaaacgc gggaatgcga attttacgag aacgaactgg aagatttgtg gttgccgctg
    44401 ctcaatcgct tgcgagtgcc gattgaacag gcgttgcgcg atgcgcgtct gaagccgagt
    44461 caaatcgaca gtctggtgct ggttggcggc gcgtcacaaa tgccgctggt gcagcgaatc
    44521 gccgtgcgtc tgtttggcaa attaccgtat caaagttacg atccgagcac cattgtcgcg
    44581 ctgggcgcag caatccaggc cgcctgccgc ttacgcagtg aagatattga agaagtgatc
    44641 ctcactgata tttgccctta ctcgttgggc gttgaggtta accgccaggg cgtttccggc
    44701 attttctcgc cgattattga acgaaacacc actgtgcccg tgtcgcgtgt agaaacttat
    44761 tcaaccatgc acccggaaca ggattcaatt acggttaacg tctatcaggg agaaaaccac
    44821 aaagttaaaa acaacattct ggtggaatcc ttcgatgtgc cgttgaagaa aaccggggct
    44881 tatcagtcga ttgatattcg ctttagttat gatatcaacg ggttgcttga agttgacgtg
    44941 cttctggaag acggcagcgt taagtccaga gtgattaacc acagcccggt aacattgagc
    45001 gcgcagcaga ttgaagagag tcggacgcgg ttatccgcat tgaaaattta tccgcgcgat
    45061 atgctcatca atcgcacctt taaagccaaa ctggaagagt tgtgggcgcg ggcgctgggt
    45121 gacgagcgag aagagattgg ccgggtgatc accgattttg atgcggcgct gcagtcaaac
    45181 gatatggccc gcgtcgatga ggtcaggcgg cgggcgagcg tttatttagc cattgagacc
    45241 tcataatcac ggctgccgga tgcggcgtaa atgctgcatc cggcatcggc gcaaaaaaca
    45301 catcgtcaac taaacaacat cgcgattttg ccgagagcat aacctataac cagcaccgtt
    45361 aaactgattt tgataatgct ggtaatgctc tgtggttttt caccctcatc atttttttct
    45421 tcatggtttt caggtgttgg ctcatcgaca tcttcatcct ccggctcgag ttttactttc
    45481 gtcaacagca tccgggtgat aatggcctct aatggatgag catctccgcc ttccagcctt
    45541 gtattaagac gggccagcat ctcttgtttc ggaaacgctt caagtactac ctgttccgct
    45601 acccgataca aatccagtaa cggtgaatct tcttctgtct cgctaccgac tctcgatttc
    45661 caccacgccc atgctttgcg tttgaagaat aacgactcaa taaacgattg acctaacggc
    45721 gataattttc cgctatgtaa aatttccgcc cataacagcc gcgtccgggc gctctcacca
    45781 ggaacatatt tcagcggttg accttgcgca tctaccatgc tccgcgcttc gataaccatc
    45841 actaataacg cgaaataatc cgggcaatga cgcttgctcc actgcaacaa caaattatca
    45901 gcttgtgtag atgggtaact ttcccagtac gcgccgagat cagcaagcag gctttccccc
    45961 tcgccgcagt acaaacgttg tgcacactgg taataccggg catcttcgct ttctggttct
    46021 tccgcttgcc actgtttggc tacaggaatg agttccacca tacctgtttg caccgagctg
    46081 taccagcgca acagtttgcg atgtaacttt gcatcatcag gaatgaacca cggtccttcc
    46141 atcgccaaaa acgcagtgac atagtcagga tggtaacggc agcaacgttc cagcgcgaag
    46201 taatattcaa cagtctggtt ctgtaacgcg acgggatatt ccagaaggct aaggaaatcg
    46261 aaacaatcac cagcctgaat ggcctcaaga aaagcctccc tttcttcctc atccattcct
    46321 tcgctgtcat taaacgaatg ccagttcaaa cgctctgcca acaaagaagc acaatttaat
    46381 gacagatatc gcgcttctat agcgattgca cacagcggcc agcgtaattg atcgacatcc
    46441 tccatgttcc aggtatttaa ttgctgaata aatttctgcc aggcggaggg ctgaaaacga
    46501 tcactttctg aatccagtaa tgtcctgaat gcacgtagta tttcatgttc agcggtggca
    46561 tctttttctt catcatctgc ttcctcgaca ggatttaccg ccagtcgcag cgcctcttca
    46621 taggcctggc gtaatgcttt aaacccctgc ggatcggttt cgggatgaca caacggtaag
    46681 cgagcaagat aagcctggcg gataatgtct atttgcgtcg tgctttcaat ttcgaggatt
    46741 tgccaacatg ttttcatagg tcgctcacat ccttaagcgt taccgggcat tcaggctgga
    46801 cagttaatgg ctgccaggga agcgtggcat agaaacggtc gcttcccgct gccagctctt
    46861 cgaacatccg actcccggcg ctatcgtatt tgtacagggt tagcgcctcg cgtagcgaag
    46921 cgttgtcttc gcactgagaa gattgccagt agagacgccc tagtgaatag gcagaaaaat
    46981 agtgcatcca gctgtgatag taatggtgag ctcgtgcata gacgcgcgat tgcagccata
    47041 aactctcttc ttccgtcagc cagttgttga gcacaccggc ccgtgacaga aatcccattc
    47101 gaacataatc ccaggctttg attccccctt ctccacagca catagcggtt tgggcaacga
    47161 attgggtata aatcaaccct ctctccgagc caccagcaat taatgctttc cactcttcag
    47221 gggaagagcg ttgccatgtc agatataagc cagctaaatc attggcatga ccatcatcag
    47281 tcatctgata aatagtctga attaaatcat cacgattatt tattccccag gatgatgaga
    47341 gatcgaattc ttttatcccg gtatacattt ttggcgagtc atgcgcgggt ttgcaactct
    47401 gattaagtat ttccatgggc gcagaaagcg caaaaagaag atattgttct tccttattca
    47461 ttttttaata atcccttaag tgcattaact gactactttt ttattattga tgagtataaa
    47521 cttgacgctt tcaaaataaa aagaaaatcg aagcattcac acatgaataa aaaattaatg
    47581 tatatattcg caatttttat agttgcagca attacctgta ttagccaacc caagaaaacg
    47641 atgttgcgtg ataaagccat ggtgaattat gcctttgatt atttaagatc accaggcagt
    47701 ctaccgttaa ccacggcagc cacggagctt tccgcgattc atggtcactc aacatcgcaa
    47761 tatcgccttg gcgaatttta tcttcatggt agcgacggta aaccactgga ttatacacag
    47821 gcgagatact ggtatgagca atcagcagaa caggaaaatc cgcgcgcaca aagcaaactg
    47881 gggtggatct acctgaaagg cctgggagtc aaacccgata cccgtaaagc aattctctgg
    47941 tataaggaag cagctgaaca agggtatgct catgctcaat atactttggg tttgatctac
    48001 agaaatggca ctggtattaa tgttaaccat tatgaatctc aaaaatggtt aaaactggcc
    48061 gccaaacaac attacaaaaa tgcggaaaga ttacttgccg gacttcccgc acattaacgt
    48121 cgactgtcac ccgaagagat ggagtatttt ccctatcaaa ccggcaaaga taaaaatgta
    48181 gaaaataatt ttgatgacgc ccagacagcc cagcccgcca tcatcaacgg gcattggctg
    48241 cggttgcggc tccggctcga cggtttcctg accggtaaaa ttcgccgcct gaattaataa
    48301 tcgacgaata attaatgctt ccagtggaca acgatcttct gcatcaaggc ggctcacaat
    48361 cggcattaat gattcttctg tcggttggct ggcctgcact aattgtttgg caactaaata
    48421 taatggccat aagggatgtt ctgtatcacg cgtgagtgag atccgcggct tacgtccttt
    48481 gtgcaaaacc atctcgacca gtgaacgccc cagcgcagag agtttttcat cgctgagtat
    48541 ttcgtataaa tgaaaacgcg tacgggcgct gtcgccgggg acataaagta gcggttttcc
    48601 ctgatcgtta accagatcac gcgcttcaat catcatcacc actaatgggt aataatagac
    48661 ccgatgctga cggcaccatt gcaacattaa agcatccgcc tgggtggagg gatactcgcg
    48721 ccagtagtaa caaagttctg ccagcaggct ttcgccttct ccacaataga cgcgttgtgc
    48781 gtattcataa taacgcgcat cttcattatc aggttcagct tcccgccacg accgggcgta
    48841 atccaggagt tcggggattc ccaggcggct tgcgctatac cagcggagta aatttctgtg
    48901 cagttttgca tcatcgggga taatcactgc gcgatgttgc gctaaataca atgtcagcca
    48961 ttctggatgc gatgaccaaa tccgagcgaa catttggtaa aaatcgatgg tgtcattttg
    49021 cacggcaact ggcagatgca gaatggtctg gaagttaaaa acattcccct tcgcaatggc
    49081 atataaaaag gattccagtt cttcttcgtc tatttccccg acgttttcct cctgcaacca
    49141 ccgcaatctt tctgctaata acaccacgca ctcgaaggat aaatgggcag tgttcatggc
    49201 gattgtgcac agcgaccagc gtaattcatc aatctcttcc atcgagcaat aatttaattg
    49261 ctgaatgaat cgctgccagg cggagggcag aaaacgttcg ctatcagagg caagtaacgc
    49321 gcgaaaggcg agcagaattt catgttctgc tacctcatat tcttctggtt gccaaacaga
    49381 tttagcaggc gactgcgcaa tccgtagcgc ctcctcatac gcctgacgaa gttgtttaaa
    49441 accctgcgga tcggtttctg gatgaaagga cggtaacagc gccagataag cgcggcggat
    49501 aatatcgacg tcagtcgttt cctctatatc gaggatcttc cagcaatttt tcataagtcg
    49561 ctcatatcct taagcgtttc cgggcatgtg gggtagtcag ccagtggttg ccaggacaag
    49621 gtagggtaat acgcatcatc actggcaaat aattgataaa acatatcatt gccggaatcg
    49681 tcgtattctt tacgcgctaa gaattcgcgt aataaaggca gatgatcatc ttcgggagac
    49741 tgccaatatt gccgaccaaa ggtataaccg gcaaagtatt gccgccagtt actataataa
    49801 cgtagagccc gtagatgaat acgcgactgg atccatagac tttcttcttc actcaaccag
    49861 ttattaagta cgcccattcg acttaaaaaa cccatccgga cataatccca tgctttaatt
    49921 cctccctcac cacagcattc ggctgtgctg gcgacaaatt gcgcataagc ctgaccttgc
    49981 tcatttaatt cagcaagata gtcacgccat tcacatggag aataacgaaa ccagcggtga
    50041 taaaaccctg ccaaccgagc ggcatgaccg ttctcgatca ttcggtgaat tatgcgcagc
    50101 aggtcatcac ggtcatcaat tccccatgag gaagaaaggt caaaaaagca tgtacctaaa
    50161 tacattttgg gggatgaatg cgaaggtaaa cagcattcat tgcgtatttc catcggagtg
    50221 gaaagtgcaa acaatatttt ttgcgacccc atgtccatat aatttatcct taaacattca
    50281 tttaatgaat gtaaactaca tttcgaggct cagttattta tacttttctg acgcaacata
    50341 atacgcataa aataaggctt acgcaaaaat ggaacacata aaaatataat atattttatc
    50401 tacaaccata aggaaatgat aatgattttt acgtcaagtt gctgcgataa tttatcaata
    50461 gatgagatta tcgaacgtgc tgaaaaaggc gattgcgagg ctcaatatat tgttgggttt
    50521 tattataatc gcgatagcgc aattgactct ccggacgacg aaaaggcctt ttactggctg
    50581 aagctggccg ctgagcaagg tcattgtgaa gcacagtatt ccttagggca aaagtatacc
    50641 gaggataaaa gccgtcataa agataatgag caagccatct tctggctgaa aaaagctgcc
    50701 ctacaaggcc atactttcgc ttccaacgcc cttggctgga tactggatcg tggagaagac
    50761 cccaactata aagaagcggt tacctggtat cagatagccg cggagagcgg aatgtcttat
    50821 gcgcaaaata atcttggatg gatgtacaga aatggcaacg gagtcgcaca agactatacg
    50881 ctggcatttt tctggtacaa acaagctgca ttacaaggcc atagttacgc acaagacaat
    50941 ctggccgatc tttataaaga cggagaaggc gttgctcaaa acaagacact cgccgcattc
    51001 tggtatttga aaagcgcaca gcagggtaat cggcacgccc agtttcaaat tgcatgggat
    51061 tataacgctg gcgaaggggt ggaccaggac tataagcaag cgatgtactg gtatctgaag
    51121 gctgccgctc aggaaagcgt cggcgcttac gtcaacatcg gttatatgta taaacacgga
    51181 caaggcgttg agaaagatta tcaggctgcc tttgaatggt ttacgaaagc cgctgaatgc
    51241 aatgacgcca ctgcctggta taacctggcc attatgtatc actacggaga aggaagacct
    51301 gtcgatctcc gacaggctct cgacctgtat cgtaaagttc agtcatccgg aaccagagat
    51361 gtcagtcagg aaattcgtga gactgaagct ttactgtaga aataaaaaca agggccgggg
    51421 aatttcattc tgcccatatt ctctttattg ctgtgcgaaa tcagactatt acggctcaaa
    51481 cgggcgtctc tggaactggt cataaccaca tttcgggcat agcgtcagca cttccggtgt
    51541 gtagatcggg agatggaagt gacatttctc gcagaccaga tttcccagcc cgaccacttc
    51601 tccgctgtga taaaccccat gatgattgag gtcctggaaa atttcgcgcc attcaagctg
    51661 cgttttatcg gtgatgtctg ccagctcctg ccacaagctt tctttaatca cccgcataaa
    51721 gacgctgtca gattcttctt tcaggctctc ttcatagctc atggcgaact cttccaggtc
    51781 acgtctgaca gctcgcgtca gctcatcgat ctcggttcgc gttaactccc ctgtttttat
    51841 tacgcgctcg cgcgcctgtt ccaccagtgc gtcgatatca cgttcgccat tgcgcaggcg
    51901 ttcgttcagt gacgcgacca gttcacggta atattgagca accttgttca tcgtttcatc
    51961 tcccgggtcg ttaactgact ataataatag acgttctttg ttccttgctt tgctaatacg
    52021 cccacaaact ctgtaaatta atgtacgaat aatccgggca tcatttcaac gtcgagtgcg
    52081 cgaaaggctg ttttgacgcg gacgtttggg ctatgctatg cggatctgaa aaaccacctc
    52141 aacgctacat ttgtagccgt attgaaaaca ggaccactgg ctgccatgca agagcaatac
    52201 cgcccggaag agatagaatc caaagtacag cttcattggg atgagaagcg cacatttgaa
    52261 gtaaccgaag acgagagcaa agagaagtat tactgcctgt ctatgcttcc ctatccttct
    52321 ggtcgactac acatgggcca cgtacgtaac tacaccatcg gtgacgtgat cgcccgttac
    52381 cagcgcatgc tgggcaaaaa cgttctgcag ccgatcggct gggacgcgtt tggtctgcct
    52441 gcggaaggcg cggcggtgaa aaacaacacc gctccggcac cgtggacgta cgacaacatc
    52501 gcgtatatga aaaaccagct caaaatgctg ggctttggtt atgactggag ccgcgagctg
    52561 gcaacctgta cgccggaata ctaccgttgg gaacagaaat tcttcaccga gctgtataaa
    52621 aaaggcctgg tatataaaaa gacttctgcg gtcaactggt gcccgaacga ccagaccgta
    52681 ctggcgaacg aacaagttat cgacggctgc tgctggcgct gcgataccaa agttgaacgt
    52741 aaagagatcc cgcagtggtt tatcaaaatc actgcttacg ctgacgagct gctcaacgat
    52801 ctggataaac tggatcactg gccagacacc gttaaaacca tgcagcgtaa ctggatcggt
    52861 cgttccgaag gcgtggagat caccttcaac gttaacgact atgacaacac gctgaccgtt
    52921 tacactaccc gcccggacac ctttatgggt tgtacctacc tggcggtagc tgcgggtcat
    52981 ccgctggcgc agaaagcggc ggaaaataat cctgaactgg cggcctttat tgacgaatgc
    53041 cgtaacacca aagttgccga agctgaaatg gcgacgatgg agaaaaaagg cgtcgatact
    53101 ggctttaaag ctgttcaccc attaacgggc gaagaaattc ccgtttgggc agcaaacttc
    53161 gtattgatgg agtacggcac gggcgcagtt atggcggttc cgggtcacga ccagcgcgac
    53221 tacgagtttg cctctaaata cggcctgaac atcaaaccgg ttatcctggc ggctgacggc
    53281 tctgagccag atctttctca gcaagccctg actgaaaaag gcgtgctgtt caactctggc
    53341 gagttcaacg gtcttgacca tgaagcggcc ttcaacgcca tcgccgataa actgactgcg
    53401 atgggcgttg gcgagcgtaa agtgaactac cgcctgcgcg actggggtgt ttctcgccag
    53461 cgttactggg gcgcgccgat tccgatggtg acgctggaag acggtaccgt aatgccaacc
    53521 ccggacgacc agctgccggt gatcctgccg gaagatgtgg tgatggacgg cattaccagc
    53581 ccgattaaag cagatccgga gtgggcaaaa actaccgtta acggtatgcc agcgctgcgt
    53641 gaaaccgaca ctttcgacac ctttatggag tcctcctggt actatgcgcg ctacacttgc
    53701 ccagagtaca aagaaggtat gctggattcc gaagcggcta actactggct gccggtggat
    53761 atctacattg gtggtatcga acacgccatt atgcacctgc tctacttccg cttcttccac
    53821 aaactgatgc gtgatgcagg catggtgaac tctgacgaac cagcgaaaca gttgctgtgt
    53881 cagggtatgg tgctggcaga tgccttctac tatgttggcg aaaacggcga acgtaactgg
    53941 gtttccccgg ttgatgctat cgttgaacgt gacgagaaag gccgtatcgt gaaagcgaaa
    54001 gatgcggcag gccatgaact ggtttatacc ggcatgagca aaatgtccaa gtcgaagaac
    54061 aacggtatcg acccgcaggt gatggttgaa cgttacggcg cggacaccgt tcgtctgttt
    54121 atgatgtttg cttctccggc tgatatgact ctcgaatggc aggaatccgg tgtggaaggg
    54181 gctaaccgct tcctgaaacg tgtctggaaa ctggtgtacg agcacacagc aaaaggtgat
    54241 gttgcggcac tgaacgttga tgcgctgact gaagatcaga aagcgctgcg tcgcgatgtg
    54301 cataaaacta tcgctaaagt gaccgatgat atcggccgtc gtcagacctt caacaccgca
    54361 attgcggcga ttatggagct gatgaacaaa ctggcgaaag caccgaccga tggcgagcag
    54421 gatcgcgctc tgatgcagga agcactgcag gccgttgtcc gtatgctcaa cccgttcacc
    54481 ccgcacatct gcttcacgct gtggcaggaa ctcaaaggcg aaggcgatat cgacaacgcg
    54541 ccgtggccgg ttgctgacga aaaagcgatg gtggaagact ccacgctggt cgtggtgcag
    54601 gttaacggta aagtccgtgc caaaatcacc gtcccggtgg acgcaacgga agaacaggtt
    54661 cgcgaacgtg ctggccagga acatctggta gcaaaatatc ttgatggcgt tactgtacgt
    54721 aaagtgattt acgtaccagg taaactcctc aatctggtcg ttggctaagc gcgggaggaa
    54781 gcgtgcgata tctggcaaca ctgttgttat ctctggcggt gttaatcacc gccgggtgtg
    54841 gctggcatct gcgtgacacc acgcaggttc cttccactat gaaggtcatg atcctggact
    54901 caggcgatcc gaacgggcca ttaagccgtg cggtgcgtaa ccagttacgt ctgaatggtg
    54961 tcgagttgct tgataaagaa accacgcgta aggacgttcc atccctgcgt ttgggtaaag
    55021 tgagcatcgc gaaagatacc gcatcggtat tccgtaacgg tcaaacagca gagtatcaga
    55081 tgatcatgac ggtgaatgcg accgtgttga tccccggccg tgatatctac ccgattagcg
    55141 ccaaagtctt ccgttcattc ttcgataacc cgcaaatggc gttagcgaaa gataacgaac
    55201 aagacatgat cgtaaaagag atgtacgacc gtgctgccga acagctgatt cgtaagctgc
    55261 caagcatccg tgctgcggat attcgttccg acgaagaaca gacgtcgacc acaacggata
    55321 ctccggcaac gcctgcacgc gtctccacca tgctgggtaa ctgatgattc ggttgtaccc
    55381 ggaacaactc cgcgcgcagc tcaatgaagg gctgcgcgcg gcgtatcttt tacttggtaa
    55441 cgatcctctg ttattgcagg aaagccagga cgctgttcgc caggtagctg cggcacaagg
    55501 attcgaagaa caccacactt tttccattga tcccaacact gactggaatg cgatcttttc
    55561 gttatgccag gctatgagtc tgtttgccag tcgacaaacg ctattgctgt tgttaccaga
    55621 aaacggaccg aatgcggcga tcaatgaaca acttctcaca ctcaccggac ttctgcatga
    55681 cgacctgcta ttgatcgtcc gcggtaataa attaagcaaa gcgcaagaaa atgccgcctg
    55741 gtttactgct cttgcgaatc gcagcgtgca ggtgacctgt cagacaccgg agcaggctca
    55801 gcttccccgc tgggttgctg tgcgcgcaaa acagctcaac ttagaactgg atgacgcggc
    55861 gaatcaggtg ctctgctact gttatgaagg taacctgctg gcgctggctc aggcactgga
    55921 gcgtttatcg ctgctctggc cagacggcaa actgacatta ccgcgcgttg aacaggcggt
    55981 gaatgatgcc gcgcatttca ccccttttca ttgggttgat gctttgttga tgggaaaaag
    56041 taagcgcgcg ttgcatattc ttcagcaact gcgtctggaa ggcagcgagc cggttatttt
    56101 gttgcgcaca ttacaacgtg aactgttgtt actggttaac ctgaaacgcc agtctgccca
    56161 tacgccactg cgtgcgttgt ttgataagca tcgggtatgg cagaaccgcc ggggcatgat
    56221 gggcgaggcg ttaaatcgct taagtcagcc gcagttacgt caggccgtgc aactcctgac
    56281 acgaacggaa ctcaccctca aacaagatta cggtcagtca gtgtgggcag agctggaagg
    56341 gttatctctt ctgttgtgcc ataaacccct ggcggacgta tttatcgacg gttgatatga
    56401 aatctttaca ggctctgttt ggcggcacct ttgatccggt gcactatggt catctaaaac
    56461 ccgtggaaac gctggcgaat ttgattggtc tgacgcgggt cacaatcatc cctaataatg
    56521 ttcctccgca tcgtccccag ccggaagcga acagcgtgca gcgtaaacac atgcttgaac
    56581 tggcgattgc cgacaagcca ttatttactc ttgatgaacg cgagctaaag cgcaatgccc
    56641 cctcttacac tgcgcaaaca ctgaaagagt ggcggcagga acaaggaccg gacgtgccgc
    56701 tggcgtttat tattggtcag gattcattgc tgacctttcc gacctggtac gaatacgaaa
    56761 cgatactcga caatgcacat ttgatcgtct gtcggcgtcc aggttaccca cttgaaatgg
    56821 cgcaaccgca ataccagcaa tggctggaag atcatttgac acataacccg gaagatcttc
    56881 accttcagcc tgccggtaaa atttatctgg ctgaaacgcc gtggtttaac atctcggcga
    56941 ccatcatccg cgaacgtttg caaaacggtg aatcgtgtga ggatttattg ccggaaccgg
    57001 tattgactta cattaaccaa caaggcttgt atcgctgata cctgtcgtta cggagggcat
    57061 gatgcgactg tggttaattc gtcatggcga aacacaagcg aatgtcgatg gtctgtacag
    57121 cggtcatgcg cccacacccc tgaccgcacg cggtattgag caagcgcaaa atctgcatac
    57181 actgctagat gatgtttcct ttgacttggt tttatgcagt gaactggaac gggcacagca
    57241 taccgcgcga ctggttctca gtgaccgcca gctccccgtt catatcatcc ctgaactcaa
    57301 cgaaatgttt tttggcgact gggagatgcg acatcatcgc gacctcatgc aagaagatgc
    57361 cgaaaactat agcgcgtggt gcaatgactg gcagcatgct atccccacga acggtgaagg
    57421 atttcaggca ttttcgcaac gtgtggaacg ctttatcgca aggcttagtg aatatcagca
    57481 ctatcagaat attttaatcg tcagccatca gggcgtactg agtctgttaa ttgctcgttt
    57541 aattggcatg cctgccgagt ctatgtggca ttttcgcgtt gaccaggggt gttggagcgc
    57601 cattgatatc aaccaaaaat tcgcgacgct acgcgtcctc aatagccgtg ccatcggggt
    57661 cgagaacgca tgacttttct gtttttttac gggtaagccg caacgaccat tgacagcccc
    57721 gggcaggctg atattctccg cagccagact ttttccgcca gacacgactt tgtagaaatt
    57781 gttttacaaa aatggcgatg caatctgcgg cgcggggtgg gatgatagcc cactttcgaa
    57841 agccgattcg gcgacaattg tcccgaaatc gcctctggtt caggtatact gacagaccat
    57901 ttttatctat ttgattcacc cagggggaaa acttgcaggg taaagcactc caggattttg
    57961 ttatcgacaa aattgatgac ctcaaaggtc aggacatcat cgccttagac gttcaaggca
    58021 aatccagcat caccgactgc atgatcatct gtacgggtac gtccagccgt catgttatgt
    58081 ccattgctga ccacgttgtg caggagtctc gcgcagcggg cctgttaccg ctcggcgtag
    58141 aaggtgaaaa cagcgccgac tggattgtcg tggatttggg cgatgtgatt gtccatgtca
    58201 tgcaggaaga gagccgtcgc ctgtatgaac tggaaaaact ctggagttaa tgcgtgaagc
    58261 tgcaacttgt cgccgtggga acgaaaatgc cggactgggt acaaaccggt tttaccgagt
    58321 acctgcgtcg ttttccgaaa gatatgccct tcgagctgat tgaaattccg gccggcaaac
    58381 gcggcaagaa tgcggacatc aagcgcatac tcgacaaaga gggtgagcag atgttggcgg
    58441 ccgcaggcaa aaaccgcatt gtcaccctcg atattccagg caagccctgg gatacgccgc
    58501 agttagccgc tgagctggaa cgctggaagc tggatggtcg cgacgtaagt ctactgattg
    58561 gcgggcctga agggttgtcg cctgcctgta aagcggcggc tgagcagagc tggtcgctgt
    58621 cggcgcttac cctcccccat ccgctggttc gcgtgctggt cgcagagagt ctgtaccggg
    58681 cgtggagcat caccaccaac catccttatc accgtgagtg ataagggagc tttgagtaga
    58741 aaacgcagcg gatgaaacta cagaactctt ttcgcgacta tacggcagag tccgcgctgt
    58801 ttgtgcgccg ggcgctggtc gcctttttgg ggattttgct gctgaccggc gtgcttatcg
    58861 ccaacctgta taatctgcaa attgttcgct ttaccgacta ccagacccgc tctaatgaaa
    58921 accgcattaa gctggtgcct atcgcgccca gccgcggcat tatctacgac cgtaacggta
    58981 tccctctggc cctcaaccgc actatctacc agatagaaat gatgccggag aaagtcgata
    59041 acgtgcagca aacgctggac gctttgcgca gcgtggtaga tctgaccgat gacgatattg
    59101 ctgcattccg aaaagagcgc gcacgttcac accgtttcac ctctattccg gtgaaaacta
    59161 acctaaccga agtacaagta gctcgctttg ccgtcaatca gtaccgtttt ccgggtgtcg
    59221 aagttaaagg ctataaacgt cgttactatc cttacggttc ggcgttgacc cacgtcatcg
    59281 gctatgtgtc gaaaatcaac gataaagacg tcgaacgcct gaataatgac ggcaaactgg
    59341 ccaactatgc ggcaacgcat gatattggta agctgggcat tgagcgttac tatgaagacg
    59401 tgctgcacgg tcagaccggt tatgaagagg ttgaagttaa caaccgtggg cgtgttatcc
    59461 gccagttaaa agaagtacca ccgcaagccg gacacgatat ttacctgacg ctggatctca
    59521 aactccagca atatattgaa acgctgctgg cgggtagccg cgcagctgtg gttgttaccg
    59581 aaccgcgtac aggtggggtg ctggcgctgg tttccacgcc tagttatgac ccaaacctgt
    59641 ttgttgacgg tatctccagc aaagattatt ccgccttgct gaacgacccg aatacaccgc
    59701 tggtgaaccg cgccacacag ggggtttatc ctcccgcgtc tacagttaaa ccctatgtgg
    59761 cggtttcggc attaagcgcc ggggtgatca cgcgcaatac gacgctgttt gacccaggct
    59821 ggtggcaact accagggtcg gaaaaacgtt atcgtgactg gaaaaaatgg ggccacgggc
    59881 gtctgaatgt cacaagatcg ctggaagaat ctgcggatac cttcttctat caggtggcct
    59941 acgatatggg gatcgatcgc ctctccgaat ggatgggtaa attcggttat ggtcattaca
    60001 ccggtatcga cctggcggaa gaacgttccg gcaacatgcc tacccgcgaa tggaaacaga
    60061 aacgctttaa aaaaccgtgg tatcagggtg acaccattcc ggttggtatc ggccagggtt
    60121 actggacagc gaccccaatc cagatgagta aggcactgat gatcctgatt aatgacggta
    60181 tcgtgaaggt tccgcatttg ctgatgagca ccgccgaaga cggcaaacag gtgccatggg
    60241 tacaaccgca tgaaccgccc gtcggcgata ttcattccgg ttactgggag ctggcgaaag
    60301 acggtatgta cggtgttgct aaccgcccta atggtacggc gaataaatac tttgccagcg
    60361 caccgtacaa aattgcggcg aaatctggta ccgctcaggt cttcggtctg aaagcgaacg
    60421 aaacctataa tgcgcacaaa attgccgagc gtttacgtga ccacaaactg atgaccgcct
    60481 ttgcgccata caacaatccg caagtggctg tcgccatgat tctggagaac ggcggcgcgg
    60541 gtccggcggt tggtacgctg atgcgccaga tcctcgacca cattatgctg ggtgataaca
    60601 acaccgatct gcctgcggaa aatccagcgg ttgccgcagc ggaggaccat taatcatgac
    60661 ggataatccg aataaaaaaa cattctggga taaagtccat ctcgatccca caatgctgct
    60721 gattttactg gcattgctgg tttacagcgc cctggttatc tggagcgcca gcggtcagga
    60781 tattggcatg atggagcgta aaatcggcca aatcgcgatg ggtctggtca tcatggtggt
    60841 gatggcgcaa attcctccac gcgtttatga aggctgggcc ccctatctct atatcatctg
    60901 tattattttg ctggtggcgg tagatgcttt cggagccatc tctaaaggcg ctcaacgctg
    60961 gctggacctc ggtattgttc gttttcagcc gtcggaaatt gccaaaatag ccgtaccact
    61021 gatggttgcg cgctttatca accgcgacgt ttgcccgcca tcgttgaaga atactggcat
    61081 cgcgctggtg ctgatattta tgcccacgct gctggtggct gcacagcctg acctgggaac
    61141 atcaatcctc gttgcgcttt ccggtctgtt tgtactgttc ctctctggcc ttagctggcg
    61201 tctgattggc gtcgcagtag tgctggtagc ggcgttcatt ccgattctgt ggttcttcct
    61261 gatgcatgat taccagcgcc agcgcgtaat gatgctcctg gacccggaat cagacccact
    61321 cggcgcgggc tatcacatta ttcagtctaa aattgctatt ggctccggcg gattacgcgg
    61381 caaaggctgg ctgcacggca ctcagtcaca gcttgaattt ctccccgaac gccatactga
    61441 ctttatcttc gcggtactgg cggaagagct gggattagtg ggcattctga ttctgctcgc
    61501 tctctacatt ctgctgatca tgcgcgggct gtggatagcc gccagagcgc aaaccacctt
    61561 tggtcgcgtc atggctggtg gcttaatgct gatattattc gtttatgtct tcgtaaatat
    61621 tggtatggta agcggtattc tgccggttgt aggggttccg ctcccactgg tcagttatgg
    61681 aggatcggcg ctaattgtgc tgatggctgg gttcgggatt gtaatgtcaa tccacaccca
    61741 caggaaaatg ttgtcgaaaa gcgtgtaaga ggtgcgcaat gcgtaagcag tggctcggga
    61801 tctgcatcgc ggcaggaatg ctcgcggcat gtacaagcga tgatggtcag caacagacgg
    61861 taagtgtacc gcagcctgcg gtatgtaacg gccccatagt tgaaattagc ggggcggacc
    61921 cgcgtttcga accactgaac gcgacggcaa atcaggatta ccagcgcgac ggtaaaagct
    61981 acaaaatcgt gcaggatccg tctcgattta gccaggcggg actggcggca atctatgatg
    62041 ccgaaccagg cagtaacctg acggcctctg gcgaagcttt cgatccgaca aagctgacgg
    62101 cggcccatcc aacgcttccg atccccagct acgccagaat cactaacctg gctaacgggc
    62161 gaatgatcgt ggtgcgcatt aatgatcgcg gcccttacgg caacgaccgc gttatttcgc
    62221 tttctcgcgc ggcagctgac cgtcttaaca cgtcaaacaa caccaaagtt cgtatcgatc
    62281 cgattattgt cgcccaggat ggttcgcttt ctggtcctgg tatggcttgt accacagtcg
    62341 ccaaacagac ttacgccctg cctgcacctc ccgatttaag cggtggcgcg ggaacaagtt
    62401 cagtgtctgg cccgcagggt gacattcttc cggtcagtaa ttcgacgcta aaaagcgaag
    62461 atccgaccgg cgcgccggta accagtagcg gtttcctcgg cgctccaacg accttagcgc
    62521 ctggtgtact ggaaggcagc gaaccgacgc ctgcgccaca gcccgttgtt acagcttcgt
    62581 cgacaacgcc tgcaacctcg cctgcaatgg tgacaccgca agccgcctcg caaagcgcca
    62641 gcggcaactt tatggtgcaa gtcggggccg taagcgatca ggctcgtgcg caacagtacc
    62701 aacagcaact gggacagaag ttcggcgtcc ccggtcgcgt aactcaaaat ggcgcggtct
    62761 ggcggatcca gcttggccca ttcgccagca aagccgaagc cagtaccttg cagcaacgtt
    62821 tgcaaaccga agcccaatta cagtcattta ttactaccgc gcagtagcgt aaagcaggca
    62881 tctgatgtgt caatttctgt aaatgacgtt aacaaagtca cgcggaaagt cagatgcctg
    62941 ccggtagtgc atttgctata gtagggcact tttttaattc catcacggat gtcgtagttc
    63001 agaccatgaa taccattttt tccgctcgta tcatgaagcg cctggcgctc accacggctc
    63061 tttgcacagc ctttatctct gctgcacatg ccgatgacct gaatatcaaa actatgatcc
    63121 cgggtgtacc gcagatcgat gcggagtcct acatcctgat tgactataac tccggcaaag
    63181 tgctcgccga acagaacgca gatgtccgcc gcgatcctgc cagcctgacc aaaatgatga
    63241 ccagttacgt tatcggccag gcaatgaaag ccggtaaatt taaagaaact gatttagtca
    63301 ctatcggcaa cgacgcatgg gccaccggta acccggtgtt taaaggttct tcgctgatgt
    63361 tcctcaaacc gggcatgcag gttccggttt ctcagctgat ccgcggtatt aacctgcaat
    63421 cgggtaacga tgcttgtgtc gccatggccg attttgccgc tggtagccag gacgcttttg
    63481 ttggcttgat gaacagctac gttaacgcac tgggcctgaa aaatacccac ttccagacgg
    63541 tacatggtct ggatgctgat ggtcagtaca gctccgcgcg agatatggcg ctgatcggcc
    63601 aggcattgat ccgtgacgta ccgaatgaat actcgatcta taaagaaaaa gaatttacgt
    63661 ttaacggtat tcgccagctg aaccgtaacg gcctgttatg ggataacagc ctgaatgtcg
    63721 acggcatcaa aaccggacac actgacaaag caggttacaa tcttgttgct tctgcgactg
    63781 aaggccagat gcgcttgatt tctgcggtga tgggtggacg tacttttaaa ggccgtgaag
    63841 ccgaaagtaa aaaactgcta acctggggct tccgtttctt cgaaaccgtt aacccactga
    63901 aagtaggtaa agagttcgcc tctgaaccgg tttggtttag tgattctgat cgcgcttcgt
    63961 taggggttga taaagacgtg tacctgacca ttccgcgtgg tcgcatgaaa gatctgaaag
    64021 ccagctatgt gctgaacagc agtgaattgc atgcgccgct gcaaaagaat caggtcgtcg
    64081 gaactatcaa cttccagctt gatggcaaaa cgatcgagca acgcccgctg gttgtgttgc
    64141 aagaaatccc ggaaggtaac ttcttcggca aaatcattga ttacattaaa ttaatgttcc
    64201 atcactggtt tggttaaaaa ttgaacactt gaaagtgtaa tttccgtccc catatactaa
    64261 gcatcagtaa aaaaactccc gccttctggc gggagttgct atttaattac gttacgccgg
    64321 agctgacatg aaaaccaaac ttaacgaact gcttgaattc cctactcctt ttacttacaa
    64381 agttatgggg caggcgttac ctgagctggt tgatcaggtg gttgaagtgg tacagcgcca
    64441 tgcgccaggt gactacaccc caacggtaaa accaagcagc aaaggcaact accactcggt
    64501 atctatcact atcaacgcca ctcatatcga gcaggttgaa acactgtatg aagaactggg
    64561 caaaatcgat attgtccgca tggttctgta actcgcttct ccgttacccg gctcctggtc
    64621 gggtaactcc cctctcctcg ctgtgatata cttttccccc acttttactc attctccacg
    64681 gagatgccgt tttgtatcag gataaaattc ttgtccgcca gctcggtctt cagccttacg
    64741 agccaatctc ccgggctatg catgaattca ccgatacccg cgataatagt acccttgatg
    64801 aaatctggct ggtcgagcac tatccggtat tcacccaagg tcaggcagga aaagcggagc
    64861 acattttaat gccgggtgat attccggtga tccagagcga tcgcggtggg caggtgactt
    64921 atcacgggcc gggacaacag gtgatgtatg tgttgcttaa cctgaaacgc cgtaaactcg
    64981 gtgtgcgtga actggtgacc ttgcttgagc aaacagtggt gaataccctg gctgaactgg
    65041 gtatagaagc gcatcctcgg gctgacgcgc ctggtgtcta tgtcggggaa aagaaaattt
    65101 gctcactggg tttacgaatt cgacgcggtt gttcattcca cggtctggca ttaaacgtca
    65161 atatggatct ttcaccattt ttacgtatta atccttgtgg gtatgccgga atggaaatgg
    65221 ctaaaatatc acaatggaaa cccgaagcga cgactaataa tattgctcca cgtttactgg
    65281 aaaatatttt agcgctacta aacaatccgg acttcgaata tattaccgct taattccata
    65341 catcaatggc ccaatttaca ctgggtcatt actcataata cctgctaatg ctttacatct
    65401 ccttcgcgtt tgtatatttt ctttatcact tgcagaaatg cggtgccacg ccttgctccg
    65461 taactcaccc cattgattaa cctattgaaa tggttgatta tggtgagcaa aagcgttccg
    65521 ttccaattag acataagatt attgtaattt tttaaatatt atttttccat gaataaaatt
    65581 ggagtaagtg cgtggatagt aataatcaaa ttgaaccctg tttaagcaga aagtcgtcag
    65641 aaggtaaacc acaaatattt acaaccttac gaaatattga tcttaacctt ctgactattt
    65701 ttgaagctgt atatgtacat aaagggatcg ttaatgcagc gaaagtgctt aatctgaccc
    65761 cctcggcaat cagtcagtct attcagaaac tgcgcgttat attccctgac ccattgttta
    65821 ttcgcaaagg ccagggtgtc actcctaccg catttgcgat gcatctacat gagtatatca
    65881 gtcagggcct tgagtccatt cttggcgcgc tggatatcga aggaagctat gataagcaac
    65941 gaacgataac tattgcgacc actccctcgg tcggagccct ggtccttcct gtcatctatc
    66001 gggcgattaa aactcactat ccgcagcttt tactgcgcaa cccgcccatc agcgacgcgg
    66061 aaaaccaact cagtcagttt caaaccgatc tcatcatcga taacatgttt tgcaccaatc
    66121 gtacggtgca acatcatgtt ctgttcaccg acaatatggt gttaatttgc cgcaagggaa
    66181 atccactact ctctttagaa gatgacagag agactatcga caacgctgcg catgtacttc
    66241 tgttaccgga agggcaaaat ttcagcggtc tgcggcagag agttcaagag atgtttccgg
    66301 accggcaaat caatttcacc agctacaaca ttttgacaat cgctgcactg gttgccaaca
    66361 gtgacatgtt agcgattatt cccagccgtt tttataacct gtttagtcgt tgctggccgc
    66421 tggaaaaact gccttttccg tccttaaatg aggagcaaat agatttctct atccactaca
    66481 acaaattcat cctgcgtgat cccatcctgc acggagtaat agatgttatc cgtaatgcat
    66541 tttgaaaaag tattgttaca gccagatcac agcgcagaac gataaaatgg cacaatccaa
    66601 caacaatttt acattttcgc gaccgctttg gctgctttca ggtccgtttc aatgatatac
    66661 tgccagtcgt taattcaaaa atagttgata attacaacaa tctattgaat tgaaacgctt
    66721 tccttcgtaa ttcgcaactg gaacacgcac gctatgagta aacccattgt gatggaacgc
    66781 ggtgttaaat accgcgatgc cgataagatg gcccttatcc cggttaaaaa cgtggcaaca
    66841 gagcgcgaag ccctgctgcg caagccggaa tggatgaaaa tcaagcttcc agcggactct
    66901 acacgtatcc agggcatcaa agccgcaatg cgcaaaaatg gcctgcattc tgtctgcgag
    66961 gaagcctcct gccctaacct ggcggaatgc ttcaaccacg gcacagcaac gtttatgatc
    67021 ctcggcgcta tttgtacccg ccgttgtccg ttctgtgatg ttgcccacgg tcgcccggta
    67081 gctcctgatg ccaatgaacc agtgaaactg gcgcagacca ttgccgatat ggcgctgcgt
    67141 tatgtggtta tcacctccgt tgaccgtgat gacctgcgcg atggcggtgc ccaacacttt
    67201 gcggattgta ttactgccat tcgggaaaaa agcccgcaaa tcaaaattga aactctggtg
    67261 ccggatttcc gcggtcgaat ggatcgtgct ctggatattc tgactgcaac gcccccagat
    67321 gtgttcaacc ataacctgga aaacgtaccg cgtatttacc ggcaggtacg gcctggcgca
    67381 gattacaact ggtcgctgaa gctgctggaa cgctttaaag aagcgcatcc ggaaatcccg
    67441 accaagtctg gtctgatggt gggtctgggt gaaaccaatg aagaaattat tgaggtaatg
    67501 cgcgacctgc gccgtcatgg tgtgacgatg ttaacgctgg ggcaatattt gcagccaagc
    67561 cgccatcacc tgccggttca acgttacgtt agcccggatg agttcgacga aatgaaagcc
    67621 gaagcgctgg cgatgggctt tacccatgct gcatgcggtc cgtttgtccg ctcttcttac
    67681 cacgccgatt tgcaggcgaa aggaatggaa gttaagtaat cctgtaataa ttacttacat
    67741 ctgtctgata aaaaacccgc tttttgaagc gggttttttg tatcgaacaa gatattgagg
    67801 gagcgtcctg ctcgccacgt cactctttat gagagagctt ttcagcctga agatcaacgt
    67861 ctgcgccttt tttcgccgca gcatcgtcat cattcatcgc cttcttgaac cctttaatgg
    67921 ccgctccaag gtctccgccc agcgtacgta acttcttagt cccaaacagc agaacgacca
    67981 gcgccgcaac taccagcagt ttggtaatac taatctcacc catagatacc ttcttgacat
    68041 ataaccggca acagaacgcg ccttaaaaac cagagaatat taacggtcat tcgacgcgcg
    68101 cacacaatag caatctgtaa caaggtgaat caagcgccga gtaaaaaaac atcataataa
    68161 ttgcggcggc gcaaagcgac ggttgtttaa gatgggcagt tgcgcgcgca cctgacgcac
    68221 acgttcgggc gtcacttccg ccataatgag tgcaggcatt tctgacgctg ccgcaatggt
    68281 gacgccaaag ggatctataa tccggctttg cccgatattt ttgttcccgc actcccccgc
    68341 cgccaccata taacaggtgg tatccagcgc acgagcggca agcaacgttg accagtgatg
    68401 ctctttgagc ggcccacgaa cccaggcggc aggaagtacc aggatttcag ctccctgtaa
    68461 agcctgtgcc agcgccagct ctggaaagcg taagtcataa caggtcatca gaccgacctt
    68521 catcccttcc acctccagta acggagctat ttcattacca gcatcaacat ggcgtgattc
    68581 ctgaatggca aatgcatcat agagatgcag tttggcataa cgggcgacga tgtttcctgc
    68641 ctgaagtgcc accagcatat tccatgcgcg ccccggcgtt gaaggaagat gaatcgtcag
    68701 aattgtcgtc atcatgttac gtttactttc tcgccgtaaa cgtccgagga attcgccttc
    68761 cagcagctgt gctgatttaa ccgatagatc tgcatcatga tcatcgcgcg ccagcaatgc
    68821 ttccggcagg acaaacagcg atacgtcgtt ttccgccgcc tgcgccatca acgaggcaca
    68881 aatctcagcg ttcttttccc acacagatgt aacagcaaac tgtcctgcag caaccagcat
    68941 tgccacctcc tgtcagagtc gtaaaattcg ccaacgacga ttattcggcg ttacacttat
    69001 cactcataca aatcaaatag caggattttg cagtgttaca acttctttta gcagttttta
    69061 ttggcggtgg tacgggaagc gtagcgagat ggctgttaag tatgcgattt aacccgctgc
    69121 atcaggcgat tccgttgggg acgctggcag caaacctgat tggggcattc atcataggaa
    69181 tgggattcgc ctggttcagc aggatgacga acattgaacc agtgtggaaa gtattaatca
    69241 ccaccggatt ttgtggcggt ctaacaacct tctcaacatt ttcggcagaa gtggtgtttt
    69301 tgttacaaga gggccgcttt ggctgggcat tactgaacgt tttcgtcaac cttctggggt
    69361 cttttgccat gaccgcactg gcattctggc tgttttcggc ctcaaccgta cactaaagac
    69421 caaaaaaaac ccgctgatta agcgggtttt gaattcttgc tgacgtatct tacagagcga
    69481 ttacgtttgc agcagaaggg cctttggcac cgttagtgat ttcgaactct acgcgctgac
    69541 cttcagcaag agttttaaaa ccattagtct ggattgcaga gaagtgtacg aacacgtctt
    69601 tgctgccgtc ttccggagta atgaaaccga atcctttgga ctcattaaac cacttaacgt
    69661 tacctttaat cttagacatc aaaattacct ttacatgaaa aatagacaca aatgctgtgt
    69721 cggttatcag tacaccaatt gtggtacgct ttgtccagtg caactttact aaaaagtgat
    69781 aaaagtcgcg ttatttttga tgttgcttat tttagttatt gattttaaag aagttactaa
    69841 aacttcattt gtctcaaaac tgaaagcgca tccaggcaaa gtacacattg ccattgttgt
    69901 aggtacccgg aatgtaggtc atctgaaaag tcactgggcc ataacccacg gaggccaatg
    69961 gcagtagaac cgggagaggg atgtaattcc agttatcgcg tgccgttacg ccagcggtga
    70021 atcccagacc taaatgaaaa ttttcatccg ccagcggtcg ccaggtactt tcccatccgt
    70081 atccggcaat cggttcccat ttgttccacg agtccttaaa tgccatggca tacaggccat
    70141 gccagtttcc tttttcatcc caacgcgaca ggccaaaacc gccaccccac ggtcgctcgt
    70201 tatagcgatc ggttttttct ttgtcgtaag cgaaacgtgc atgccaggtg atggcaggaa
    70261 tatataaatc ataatgttca ggctgttgcc aggtttgtgc aatattttct ctaaacgttg
    70321 tcatcctctc atctgcgtta gcaaaaactt taccaacgct gattaactga ataaaaacaa
    70381 aggaaaagat agcgacatat ttactcacgt tcatttgtga ccataaaaca tttatcaaaa
    70441 atctactact agcatagcaa agctacaatt aacataacct taatagaccc aacataaaga
    70501 ataatctgaa tagctgctct atttatttcc gcttgttcaa gttaaatatt tccgcgcatc
    70561 gattaaagat gatgcgtcgt tttctgcgat gggaatagtc aaaaaagaaa aaccaagtct
    70621 tttttgatga caaaatgcaa tcaaggaaaa attattttat ttttttaacg agatacaaca
    70681 atcatcttaa cgaagtatat aatatatatc gtcaacccat gctcatatta gattattttt
    70741 tgagcgcaca gccgcagcta acaccattgc aattaacaaa tttgcatcaa tccaccatca
    70801 atttacacac attattatgt gataattgcc aaccgctaaa tatgcgtttt gttatctatg
    70861 tataaaaaca gcaacttcaa tgtcttaatg gcagtttttc ttgattttaa tcagcattca
    70921 tcgccaattt attgggcata ttttttcctt aagctttagg aagtttttat tatttacttt
    70981 gggcctggag acaggaaaaa ttatgctgac attcattgaa ctccttattg gggttgtggt
    71041 tattgtgggt gtggctcgct acatcattaa agggtattcc gccactggtg tgttatttgt
    71101 cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt taccgtccag
    71161 ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa tattactaat
    71221 gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggctttg ccgcttacat
    71281 gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat tgcagtatat
    71341 taactcccct tacctgctga tgattgccgc ctattttgtt gcttgtctga tgtcactggc
    71401 cgtctcttcc gcaaccggtc tgggggtttt gctgatggca accctgtttc cggtgatggt
    71461 aaacgttggt atcagtcgtg gcgcagcagc tgccatttgt gcctccccgg cggcgattat
    71521 tctcgcaccg acttcagggg atgtggtgct ggcggcgcag gcttccgaaa tgtcgctgat
    71581 tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg gcatggcgat
    71641 cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc atgaaatgtt
    71701 agatgtcagt gaaatcacca ccactgcccc cgcgttttat gccattttgc cgttcacgcc
    71761 gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca tcatcactat
    71821 tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct ttaataccca
    71881 gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt ttgctaacgt
    71941 ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca tcggctttat
    72001 tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc tgatgctggt
    72061 attggtgatt ctgaccatgc tggcggcagt cacgaccggt tcaggcaatg cgccgtttta
    72121 tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc cggcgtattt
    72181 gactatcccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg tttctggcgt
    72241 agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa aacgcacctc
    72301 ggtgccggtg cttgttgggc tagtgattgt tatcgttgct acagagctga tggtgccagg
    72361 aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt tcctccggct
    72421 ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc gtgaacaatt
    72481 tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc agtggtgcgg
    72541 ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg ctctttaaac
    72601 agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggcagttc gtctttgggt
    72661 tcaccgcgag cataggcatt aaacatctca tcaatttgct tctggctggc gctatcaata
    72721 ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag tgtattgatt
    72781 cacttgaagt acgaaaaaac cgggagaaca ttggattatt cgggatctga tgggattaga
    72841 tttggtgggg cttgcaagcc tgtagtacaa attttaggcg ttaaccaatg aaacgcgaaa
    72901 gatagtaaaa aattgctttt gtttcattga aaaaacgaaa aacaaaaaca ctataaatca
    72961 tttcaatcat agcttcaaaa aacgttcaaa accgataaca accaggctgt catcaaatga
    73021 ctcatatcac aaatcagctc atgccgttta ggtatgttac atgtgtgatt atgtgaggtg
    73081 aagtatgttt tagctggctc atggttgtta tacggctttt ttcgcatctt gtggttccaa
    73141 caaaggagct acaggctctt cctgtttatg aacaccatac attgacaaaa tctcttggcg
    73201 agtttctgga ggtaatttat gcagcgccaa agctgtttgt agtgtttggc gatcaatacc
    73261 cagcgttgcc aatagggtgt caacggagat ggaggggcgt tcttgcatta atccggaaag
    73321 cccatattta gccatgttta gctctatctc tcggacttca ttttctccta gtttattgat
    73381 taacagggag aacattttca atgtatcagc attcatttct tcgatagcaa ttttttgcat
    73441 agccggtatt gccgggatat attcaacccc aggcccgcga cgcccttctt gtgaacgctt
    73501 cggccatcct tctttctttg ctcgtttatg caccccctgt attgtcccag gcattccatc
    73561 cagttcagta atttgtttgg gtgtcatcca ctggtttatc tcatttgcgc ttttcataaa
    73621 ctaagtttac cagatttatc tatataaaaa tcaatggatt acaaaataaa ccacattaac
    73681 tgatataaac cggtttggtt tatttggtaa tgtataaaac agataatcaa gacgaattat
    73741 ttgttttggt aaccctttat catacgcaat atggttggtg tgcgaaagat gagtaacgat
    73801 tggcatcggg ccgacgtgat tgcggccctc aaaaaaacag gaacccctct ggcaaaattg
    73861 tccagacgtt caggttattc aaccgggacg cttgcaaatg cattagtcag gccatggcct
    73921 aaaggtgaac gcattattgc tgacgcctta aacatgaaac ctgaggaaat ttggcctagt
    73981 agatatctaa gggatcacaa tgagagagtg gatatcagca attgaatgca tgggtttatc
    74041 tggtttacct ggttcattaa tgggggttca caaaagagct cagagagaag gatggctcaa
    74101 gagaagaaga gaaggaatta gaggggttgc ttacgaatac catatatctt ctcttcctaa
    74161 cattgctcga gctgaagttt tgctccaaca aggaaaattt aaaaccagtc agggatgttt
    74221 tgaaatcgcc cgccccacgc tggaagctca tgattatgat cgtgaggcac tatggagcaa
    74281 atgggataaa gccagcgatt cccagcgcag cctggctgaa aaatggttgc cctcaattca
    74341 ggccacagac gaaatgctga accaggggat ttcaacgaaa acggcttttg cgaccgttgc
    74401 agggcattac caggtcagcg catccacttt gcgggacaag tattaccagg tacagaagtt
    74461 tgcgaagcct gactgggcgg ctgcacttgt tgatggacgt ggagcatccc gtcgcaatgt
    74521 tcacaaaagt gaatttgacg aggatgcctg gcagtttctg attgcagatt atctgcgacc
    74581 ggaaaaaccc gccttccgca aatgttatga gcgtctggaa ctggcagccc gcgagcatgg
    74641 ctggagtatt ccctcccgtg ccacggcctt tcgccggatt cagcaactgg acgaggcaat
    74701 ggttgttgcc tgtcgtgaag gtgaacatgc actgatgcat ctgataccgg cacagcagcg
    74761 aactgtggaa cacctggacg ccatgcagtg gatcaacggc gacggttatc tgcataacgt
    74821 ctttgtacgc tggtttaacg gtgatgtgat ccgtccgaaa acatggttct ggcaggatgt
    74881 gaaaacccga aaaattctgg gctggcgctg cgatgtgagc gagaacattg attcaattcg
    74941 cctctcgttc atggatgttg tgactcgcta cagtatcccg gaggattttc acatcaccat
    75001 tgataacacc cgtggtgctg cgaataaatg gctgacggga ggcgcgccca atcgctaccg
    75061 ctttaaggta aaagaggacg atccaaaagg actgttttta ctgatgggtg cgaaaatgca
    75121 ctggacaagc gttgttgccg gtaaaggctg gggccaggca aaacctgttg aacgtgcttt
    75181 cggtgttggt gggcttgagg aatacgttga taagcatccg gcactggctg gcgcatatac
    75241 ggggccaaat ccgcaggcaa aacctgataa ctatggcgac cacgctgttg atgcagagct
    75301 gtttctgaaa acctttgccg aaggtgtggc gatgttcaat gccagaacag gccgtgaaac
    75361 agaaatgtgc gggggcaaac tctcgtttga tgatgttttc gagcgtgaat acgccagaac
    75421 gattgtgcgt aagccaaccg aagaacaaaa acggatgctg ttactgcctg ccgaggcggt
    75481 gaacgtttca cgcaaaggcg agtttacgct taaagttggc ggctccctta aaggcgcgaa
    75541 aaacgtttat tacaacatgg cattaatgaa tgccggagtg aaaaaagttg tggtcagatt
    75601 tgatccacag cagttacaca gcacggttta ttgag
//
LOCUS       DAJTSF010000004        72904 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000004 DAJTSF010000000
VERSION     DAJTSF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.004"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO335_000314"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO335_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6000833.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO335_000315"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO335_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6000834.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO335_000316"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO335_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6000835.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO335_000317"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO335_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6000836.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO335_000318"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO335_000318"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6000837.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO335_000319"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO335_000319"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6000838.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO335_000320"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO335_000320"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6000839.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO335_000321"
     CDS             complement(4598..5320)
                     /locus_tag="OO335_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6000840.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO335_000322"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO335_000322"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6000841.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO335_000323"
     CDS             6520..6738
                     /locus_tag="OO335_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6000842.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO335_000324"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO335_000324"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6000843.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO335_000325"
     CDS             complement(7472..7672)
                     /locus_tag="OO335_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6000844.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO335_000326"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO335_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO335_000327"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO335_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6000845.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OO335_000328"
     CDS             10101..12014
                     /locus_tag="OO335_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6000846.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="OO335_000329"
     CDS             12014..13036
                     /locus_tag="OO335_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6000847.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="OO335_000330"
     CDS             13030..13248
                     /locus_tag="OO335_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6000848.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="OO335_000331"
     CDS             13302..14171
                     /locus_tag="OO335_000331"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6000849.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="OO335_000332"
     CDS             complement(14226..14630)
                     /locus_tag="OO335_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6000850.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="OO335_000333"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="OO335_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6000851.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="OO335_000334"
     CDS             15615..17705
                     /locus_tag="OO335_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6000852.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO335_000335"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO335_000335"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6000853.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO335_000336"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO335_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6000854.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="OO335_000337"
                     /pseudo
     CDS             complement(19673..20275)
                     /locus_tag="OO335_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
     gene            complement(20265..20432)
                     /locus_tag="OO335_000338"
     CDS             complement(20265..20432)
                     /locus_tag="OO335_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6000855.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO335_000339"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO335_000339"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6000856.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="OO335_000340"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="OO335_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6000857.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="OO335_000341"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="OO335_000341"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6000858.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="OO335_000342"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="OO335_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6000859.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="OO335_000343"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="OO335_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6000860.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="OO335_000344"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="OO335_000344"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6000861.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="OO335_000345"
     CDS             28269..28436
                     /locus_tag="OO335_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR6000862.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="OO335_000346"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="OO335_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="OO335_000347"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="OO335_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6000863.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="OO335_000348"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="OO335_000348"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR6000864.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="OO335_000349"
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="OO335_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6000865.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="OO335_000350"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="OO335_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6000866.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="OO335_000351"
     CDS             complement(33764..34849)
                     /locus_tag="OO335_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6000867.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="OO335_000352"
     CDS             complement(34861..36165)
                     /locus_tag="OO335_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6000868.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="OO335_000353"
     CDS             complement(36177..36530)
                     /locus_tag="OO335_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR6000869.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="OO335_000354"
     CDS             complement(36541..37419)
                     /locus_tag="OO335_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6000870.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="OO335_000355"
     CDS             complement(37416..38642)
                     /locus_tag="OO335_000355"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6000871.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="OO335_000356"
     CDS             complement(38643..39806)
                     /locus_tag="OO335_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR6000872.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="OO335_000357"
     CDS             complement(39890..40252)
                     /locus_tag="OO335_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6000873.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /locus_tag="OO335_000358"
     CDS             complement(40269..41174)
                     /locus_tag="OO335_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000874.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO335_000359"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO335_000359"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6000875.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO335_000360"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO335_000360"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6000876.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO335_000361"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO335_000361"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6000877.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO335_000362"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO335_000362"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6000878.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO335_000363"
     CDS             complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO335_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6000879.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO335_000364"
     CDS             complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO335_000364"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6000880.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO335_000365"
     CDS             complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO335_000365"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6000881.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="OO335_000366"
     CDS             complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="OO335_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6000882.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="OO335_000367"
                     /pseudo
     CDS             complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="OO335_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="OO335_000368"
     CDS             complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="OO335_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6000883.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="OO335_000369"
     CDS             complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="OO335_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR6000884.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="OO335_000370"
     CDS             complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="OO335_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6000885.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51689..54241
                     /gene="mrcA"
                     /locus_tag="OO335_000371"
     CDS             51689..54241
                     /gene="mrcA"
                     /locus_tag="OO335_000371"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6000886.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="OO335_000372"
     CDS             complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="OO335_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6000887.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55286..57421
                     /gene="igaA"
                     /locus_tag="OO335_000373"
     CDS             55286..57421
                     /gene="igaA"
                     /locus_tag="OO335_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6000888.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57486..58154
                     /gene="yrfG"
                     /locus_tag="OO335_000374"
     CDS             57486..58154
                     /gene="yrfG"
                     /locus_tag="OO335_000374"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6000889.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58165..58566
                     /gene="hslR"
                     /locus_tag="OO335_000375"
     CDS             58165..58566
                     /gene="hslR"
                     /locus_tag="OO335_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6000890.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58591..59460
                     /gene="hslO"
                     /locus_tag="OO335_000376"
     CDS             58591..59460
                     /gene="hslO"
                     /locus_tag="OO335_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6000891.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59604..61328)
                     /locus_tag="OO335_000377"
     CDS             complement(59604..61328)
                     /locus_tag="OO335_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6000892.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61707..63329
                     /gene="pckA"
                     /locus_tag="OO335_000378"
     CDS             61707..63329
                     /gene="pckA"
                     /locus_tag="OO335_000378"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6000893.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63405..64757)
                     /gene="envZ"
                     /locus_tag="OO335_000379"
     CDS             complement(63405..64757)
                     /gene="envZ"
                     /locus_tag="OO335_000379"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6000894.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64754..65473)
                     /gene="ompR"
                     /locus_tag="OO335_000380"
     CDS             complement(64754..65473)
                     /gene="ompR"
                     /locus_tag="OO335_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6000895.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65701..66177
                     /gene="greB"
                     /locus_tag="OO335_000381"
     CDS             65701..66177
                     /gene="greB"
                     /locus_tag="OO335_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6000896.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66274..68595
                     /locus_tag="OO335_000382"
     CDS             66274..68595
                     /locus_tag="OO335_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6000897.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69033..69260
                     /gene="feoA"
                     /locus_tag="OO335_000383"
     CDS             69033..69260
                     /gene="feoA"
                     /locus_tag="OO335_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6000898.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69277..71598
                     /gene="feoB"
                     /locus_tag="OO335_000384"
     CDS             69277..71598
                     /gene="feoB"
                     /locus_tag="OO335_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6000899.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71598..71834
                     /gene="feoC"
                     /locus_tag="OO335_000385"
     CDS             71598..71834
                     /gene="feoC"
                     /locus_tag="OO335_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6000900.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72037..>72904
                     /gene="rpnA"
                     /locus_tag="OO335_000386"
     CDS             72037..>72904
                     /gene="rpnA"
                     /locus_tag="OO335_000386"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR6000901.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAA"
BASE COUNT        17570 a        19753 c        18440 g        17141 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctagtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcacgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 atcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 atttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacatcacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tggctgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aggaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgcgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gccatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggagcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact ctacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctgg gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgccctcc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgctccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaatgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc
    45301 cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac gaactgggcg
    45361 cgtagtggac ggacgttcag cggtttgcgt tttcgccgtg tcacgcgatg cattgccatt
    45421 gcgaacaggc gcaaccgttg cgggttcagt cggcaacgtg gaattgaccg ccacattgtt
    45481 cagctgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct gcacttcaac
    45541 acgttgttga ccatccgttg ccaccggggt ttgcccttga gtcggcgtag aagagatcgg
    45601 tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg ctggctgcac
    45661 gccattcgcc tgatcggtcg cattgccagc aagatcaata ctcttctcgc cagacgcggt
    45721 ttgatcgctg gaagtggtcg agggggcttt tagcgcagaa ccgataccga tgatcaacag
    45781 cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg gtttactggc
    45841 tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg tttcatcttc
    45901 ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt gcgcacgagt
    45961 cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct gcggttcgcc
    46021 acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac gacgatcgct
    46081 gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc ccactaaaag
    46141 gttaatgctt accacgttgc aattaacctg aagctaatag accgcttgat aagcggcctg
    46201 acctttcttg ttgttacgct gattgacaat cggcaatggc gttaagaaca agctcgtgcg
    46261 aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag cgcatctctc
    46321 ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc
    46381 gcggcccatt gaccggtaac ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg
    46441 cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata cccgcagcga
    46501 ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca tgaccaaagg
    46561 tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt
    46621 ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg tccagacgca
    46681 acaacgcatc cagattctct tccagccagt taaaaaacgc accgtcaaga ataatgccgt
    46741 atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt ttcagacagt
    46801 cgagatccac caccactgaa gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg
    46861 gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg
    46921 ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc
    46981 ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggc ttttgtaaca
    47041 acgccgtaaa gacggtatcg agtacagcta ggcttttata ctgctcgccg tcagggagga
    47101 taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta tcgagataca
    47161 gaggagccag ggtttcgctg gtgaccaaca tgacctgctc gcccgatttc agcggtaaga
    47221 atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta cgttccccga
    47281 gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac ccgcagacga
    47341 gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt ttgcaaccac
    47401 tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat acagcggatt
    47461 gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg
    47521 gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atggtcgttt caagataaac
    47581 gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag agccgccgcc
    47641 agtagccagc acaataccct gtttctcggt caactcattg atgacctttt cttcgcgatc
    47701 gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc cggttcgttt
    47761 ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta actggcgccc
    47821 aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt tctctgccat
    47881 tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca gagagcgccg
    47941 caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa ttatctcaat
    48001 actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct ggtcgctgca
    48061 agcgcgctgc cttgctacca ccgctctggc gataaatcac cgggtaagat tagcgtaaaa
    48121 aagacagcaa aatgccgcct gaatgataaa tcatcatcat ggaacgcagg cagcgaagag
    48181 caccaactct caaatcggta ctccttgtat gctaaatacc tgcgcgcgtc aaatagatga
    48241 aacacgttca gcataaaaac attaccgctt ttacggctgt ttactcactg gaaaccagtc
    48301 gtggcgtgat aaacaccact aactcgcgtc gttcatcttc ttttccgtca tgacgaaata
    48361 attgcccgaa ccagggaatg tcgccaagca acggtacgct atcctgaccc gatttatttt
    48421 tacgggtaaa aatgccgccc agcgccaacg tttctccgct ttttacctcg acctgcgttt
    48481 cgatctcctg cttatcaatc gccagcactt cgccatcggc ctgctgtagc acctgccccg
    48541 gaacgttctg gctgatgtgt aatttcagcc ggatgcgacc tttttgtaac accgtgggcg
    48601 tgacctccat ccccaggacg gcctctttaa attccaccga cgtcgcgcca ctttccccgc
    48661 tggaaacctg atatggaatt tcgctctcct gtttaatgct ggcaggctga agatgtgagg
    48721 ccagcagacg cggactggcg ataatatcca gctgctgttt ttgttcgagc gcggaaagct
    48781 caagatccag caagcgtccg ttgatgcgcc caatgttaaa accgacatgc gttgtcgccg
    48841 tcgctacgga gaggtcgcta ccaagcgtgg tgacttgccc aacgccacca gcgtgttgcg
    48901 catcggccag cgtccatttc acgcctaact cacgcaaact tttttcatta atggtgacaa
    48961 tatgcgccga cagctcaacc tgcccgaccg gcagatccat ttgcgctacc cactgttcaa
    49021 gcgcgcttaa cgccgttttg ttatctcgta gcaaaaggcg attggtgcgt ttatcgacgg
    49081 tcatactccc tttggcactc agtagcttct cccccgcttt cgccagttct cccgcgtcgg
    49141 cgtattgcag ggttatactg cgattttcca gcggcagatt tgcctgcgcc cgcgcctgct
    49201 ccgcctcctg gcgggcgata ttgtcattct gccaggcaat ggaatgcact gagagaatgt
    49261 tgccttcctg ccgcgttatc agtccggcgc ttttcactac agtttgtagt gcctgcttcc
    49321 agggaacatc tgttagatgt aacgacaccg taccgctgac gtctggcgac acgaccaggt
    49381 tcaacttctc ctgttcagcc agcgcctgca acacctgagc taccggaacg tcatccacca
    49441 tcagcgtcac tttttgcggc tttgccgcct gtacgccggg tatcagcatc aacagtagtg
    49501 cggctatcca ttgcttcatt tgtatctcct tgccgttgcc acaaccattg tggcggttcg
    49561 cagtttgtcc cggtacccag cgttagtacg tctggcgtca gctgtaaaat tgtccagccg
    49621 ttttccagca catcgttttg ctgcacccgt cgccatttct tttgcccgtc ttttattaca
    49681 ccgatgatgc gctcgcctcg ccctaccatc ccctgatagc gccactggct aagttcgcta
    49741 atccggcata tagatcttcc ggcgatttaa aagggtcacg cataccggtt aaaaggcaca
    49801 atgcaatacc tgccaacaac cagcgtttaa ccctcatgcg gcatctccag ttgtagcgtg
    49861 aacaaaagat catcaccttc cacgcttaac gaaaaacggc tcacgctgac gttgcgctct
    49921 gccagccgtg taaatgccga cggcactgct tcccacagcg ttttcaacgc caactcacct
    49981 ccctgcgcgg atggatgcca ggaaaccagt tgcgcgccgg ataactgaaa atccagtggc
    50041 gaaaagggca gcgttttttc ctcgctgaag ggagtggtgt ctaccaggcg atacagtgcg
    50101 gcccactgct ggtgatgact tgcccgtagt cgaattaatg cgtcgcgctc ttcatggtgt
    50161 gtcgatgata gaaaaatgag cgtaactaac atcagcaacc agaatgccca gcaaaactgg
    50221 cggaggcggg gtgatgtggc gaaccaccag tcaaagaaca tgttcatcgc taaccttcct
    50281 tgttaactga tactcaaatt gccagcgtcc ctgcgcatcc tgctgcgtgg ctccccgctg
    50341 attgagatga aaagacgcat cctggcggag tgacgtttct agtgcgttta acgcggtaat
    50401 gcttgttgtc agccccttga tctccagcgt tccctgctgc cagcttatcg ttgtcagcca
    50461 ggcgtgctcg ggtaaaagcg ccgccagtgc ttccagcgca gattgccagt cgcgggtaaa
    50521 ttgtcgctgg cgctgccgtt gcgaacgctg ttcgcgtaac tgctgttgct ccagcaaacg
    50581 tggcttcgtt atctgtaagc tgcgggcgag ttgttgttcc gcctgaagca aaactgcgtc
    50641 tatgcgcgct tcggcgctgc ctgtcagacg cagtattagc gttatcccga cggccagcag
    50701 cagaggcgca acgaacatca gcaaccagaa acgcagaaaa gcggtccggc gttgctgtcg
    50761 ccagggcaaa aaattaattg gcggattcat cagtacgcct tcccaagcgc cagccccagc
    50821 gcgatggcaa agtctccacc aggcggcggt agcggcggct gacgaacaga aacggcctcc
    50881 caggggtcaa atccgccttc gccacatatc gcgacgctgt cgggatcaac ggataacgct
    50941 gccgccagct ccttcgcgct agtcatcccc accgccagtt tgcgccccca gctatagcgc
    51001 gtcgcccaca gccactgttc gttatcacgc caggccagac attgctgatg agaaggtaaa
    51061 aaaggcagga atcgctgtaa tgcactggca tccggggtga tcgcactaac atgaacacgc
    51121 aacctttctg ccagcgtaag cagcgttgcc agctctttgc tttgcgcggc agtcacgtta
    51181 taagcggggc tgagtgagtc ttcgctgtaa tcgaagcgca gggagtccgg atccatatcc
    51241 agctcgcggg ccatcgtccc tgacagccag gccgtttgct cccgctcacc aagggacatc
    51301 gacgggcgcg gaaatgaccg ctgtaatgtg cgactggcgg gaaacgccaa cataatgtga
    51361 tgacgctgcg gcagttcgcg actccacggt aacaacgttt tagccagctg ctgcgcatca
    51421 acaatccgcc catctttgat aatgtcgtgc gccagcggca accgccacca gcgttgcaaa
    51481 aagcattctt ttgcgccccg cacgatcgca accgctgccg cttcttgctg ttgtaaatgc
    51541 aaaccaattt gccagatctt aaatgtcatt gtgatgatct ccttatcacc cgtcactctg
    51601 acgggtatat caatgcgtct ggcttgcctt tatactaccg cgcgtttgtt tataaactgc
    51661 ccaaatgaaa ctaaatggga aatttccagt gaagttcgta aagtattttt tgatccttgc
    51721 agtctgttgc attctgctgg gagcaggctc gatttatggc ctataccgct acatcgagcc
    51781 acaactgccg gatgtggtga cattaaaaga tgttcgcctg caaattccga tgcagattta
    51841 cagcgccgat ggcgagctga ttgctcaata cggtgagaaa cgtcgtattc cggttacgtt
    51901 ggatcaaatc ccaccggaga tggtgaaagc ctttatcgcg acggaagaca gccgcttcta
    51961 cgagcatcac ggcgttgacc cggtggggat cttccgtgcg gcaagtgtgg cgctgttctc
    52021 cggccacgcg tcacaagggg cgagtaccat tacccagcag ctggcgagaa atttcttcct
    52081 cagtccagaa cgcacgatga tgcgtaagat taaggaagtc ttcctcgcga ttcgcattga
    52141 acagttgctg acgaaagacg aaatcctcga gctttatctg aacaagattt accttggtta
    52201 ccgcgcctat ggtgtcggtg ctgcggcaca agtctatttc ggtaaaacgg tcgaccaact
    52261 gacgctgaac gaaatggcgg tgatagccgg gttgccgaaa gcgccttcca cctttaatcc
    52321 gctctactca atggatcgtg ccgtcgcgcg gcgtaacgtc gtactgtcgc ggatgctgga
    52381 tgaagggtat atcacccagc aacagttcga tcagacacgc actgaggcga ttaacgctaa
    52441 ttatcacgcg ccggagattg ctttctctgc gccgtacctg agcgaaatgg tgcgccagga
    52501 gatgtataac cgttatggcg aaagtgccta tgaagacggt tatcgcattt acaccaccat
    52561 cacccgcaaa gtgcagcagg ccgcgcagca ggcggtacgt aataacgtgc tggactacga
    52621 catgcgccac ggctatcgcg gcccggcaaa tgtgctgtgg aaagtgggcg agtcggcgtg
    52681 ggataacaac aagattaccg atacgctgaa ggcgctgccg acctatggtc cgctgctgcc
    52741 tgccgcagtc accagcgcca atcctcaggg agcgacggcg atgctggcgg acgggtcgac
    52801 cgtcgcattg agtatggaag gcgttcgctg ggcgcgtcct taccgttcgg atactcagca
    52861 gggaccgacg ccgcgtaaag tgaccgatgt tctgcaaacg ggtcagcaaa tctgggttcg
    52921 tcaggttggc gatgcatggt ggctggcaca agtgccggaa gtgaactcgg cgctggtgtc
    52981 gatcaatccg caaaatggtg ccgttatggc gctggtcggt ggctttgatt tcaatcagag
    53041 caagtttaac cgcgccaccc aggcactgcg tcaggtgggt tccaacatca aaccgttcct
    53101 ctacaccgcg gcgatggata aaggtctgac gctggcaagt atgttgaacg atgtgccaat
    53161 ttctcgctgg gatgcaggtg ccggttctga ctggcagccg aagaactcac caccgcagta
    53221 tgctggtcca attcgcttac gtcaggggct gggtcagtcg aaaaacgtgg tgatggtacg
    53281 tgcaatgcgg gcgatgggcg tcgactacgc tgcagaatat ctgcaacgct tcggcttccc
    53341 ggcgcaaaac attgtccaca ccgaatcgct ggcgctgggt tcagcgtcct tcaccccaat
    53401 gcaggtggcg cgcggctacg cggtcatggc gaacggcggc ttcctagtgg acccgtggtt
    53461 tatcagcaaa attgaaaacg atcagggcgg cgtgattttc gaagcgaaac cgaaagtagc
    53521 ctgcccggaa tgcgatattc cagtgattta cggtgatacg cagaaatcga acgtgctgga
    53581 aaataacgat gttgaagatg tcgctatctc ccgcgagcag cagaatgttt ctgtaccaat
    53641 gccgcagctg gagcaggcaa atcaggcgtt agtggcgaag actggcgcgc aggagtacgc
    53701 accgcacgtc atcaacactc cgctggcatt cctgattaag agtgctttga acaccaatat
    53761 ctttggtgaa ccaggctggc agggtaccgg ctggcgtgca ggtcgtgatt tgcagcgtcg
    53821 cgatatcggc gggaaaaccg gtaccactaa cagttcgaaa gatgcgtggt tctcgggtta
    53881 cggtccgggc gttgtgacct cggtctggat tggctttgat gatcaccgtc gtaatctcgg
    53941 tcatacaacg gcttccggag cgattaaaga tcagatctca ggttacgaag gcggtgccaa
    54001 gagtgcccag cctgcatggg acgcttatat gaaagccgtt cttgaaggtg tgccggagca
    54061 gccgctgacg ccgcctccgg gtattgtgac ggtgaatatc gatcgcagca ccgggcagtt
    54121 agctaatggt ggcaacagcc gcgaagagta tttcatcgaa ggtacgcagc cgacacaaca
    54181 ggcggtgcac gaggtgggaa cgaccattat cgataatggt gaggcacagg aattgttctg
    54241 attaaaaagg cgcttcggcg ccttttcagt tggctgacaa agtgcatttg tttatgccgg
    54301 atgcgggtga acgcgttatc cggctaacaa aatcgtgaaa aatcaatata ttgcaggaac
    54361 cccataagcc tgataagcgt tgtgcatcag gcaaacttca cgcatttaca ctcgcccctg
    54421 ccctttcaac cattcgcgca cgaggaacag cgcgctgaca ttacgcgctt cgttgaagtc
    54481 agggtcttcc agcaaatcca tcatatgcgc cagcggccag cgcacctgtg gtagcggctc
    54541 tggctcatcg ccttccagcg attccgggta gagatcttgc gcgaccacga tattcatttt
    54601 gctggaaaag taagacggtg ccatgctgag cttcttcaaa aaagtcagat cgttcgcacc
    54661 aaatccaacc tcttctttta gctcgcggtt agcggcttcg tagacgcttt cacccggatc
    54721 aattaatcct ttcgaaaaac ctaattcgta ggattcagtt cccactgcgt attcgcggat
    54781 caggatcagg tgatcgtcca caatcggcac aatcatcact gcttcccggt tggttggacg
    54841 cattcgttca taaacacgcc gcacgccatt gctgaactcc agatccacgc tctcgacggt
    54901 aaacagtcgg gaacgggcta cagtttcaac attcagaatg gtgggttttt gtaatgattt
    54961 gttcatcgtg ggatctatgc tgtgaaatca gcggttattg tgcgatatcg gacacgcttt
    55021 cggcaatgtg aattgcatgt tatttacatt tatgtaactt aataaataat cgtcctcaaa
    55081 tcaaattaaa agtcaatagg ttgaaataac tccaggaatt tgctgatatt ccgccttcag
    55141 agggtttgct atgatcagcg gttactgtga tgtgcttaat gatgctcaag ttaagctcca
    55201 cgcttgccga tagccaaccg cagaatcatg tattgtgtcc ggtgcgactg accacgcctg
    55261 acagactaag taagatgggg aaagcatgag caccattgtg atttttttag ctgctttgct
    55321 ggcctgctca ctacttgcgg gatggctgat aaaagtgcga tccagacggc gtcagctgcc
    55381 ctggaccaac gccttcgcgg atgcgcaaac gcgtaaactc acacctgaag aacgtagcgc
    55441 cgttgaaaat tatcttgaga gcctgacgca ggtattacag gtgcctggcc caacggaagc
    55501 tagcgcggca ccgatctctc tggcgctgaa tgccgaaagc aacaacgtca tgatgctgac
    55561 acacgctatc acacgttacg gcatctctac cgacgatccg aataaatggc gttactacct
    55621 cgattcggta gaagtccacc tgcccccctt ctgggaacag tacatcaacg atgagaatac
    55681 cgttgaactg attcataccg attcgctgcc gttggttatt tcactcaacg gtcatacgct
    55741 gcaggagtac atgcaggaaa cgcgcggcta tgccttgcaa cctgttccgt ccacgcaggc
    55801 gtcgattcgc ggggaagaga gtgagcaaat cgagctactc aatattcgca aagaaacgca
    55861 tgaagaatat gcgctgagtc gtccgcgcgg gctgcgtgaa gcgttgctga tcgtcgcctc
    55921 cttcctgatg ttctttttct gcctgattac cccggatgta tttgttccgt ggctggcagg
    55981 cggcgcatta ctgctgctgg gcgcaggtct gtgggggcta ttcgcgcccc cggcaaaatc
    56041 ctccctgcga gaaattcatt gtctgcgcgg tacaccccgt cgttggggat tgtttggcga
    56101 aaacgatcag gaacagatca acaatatttc actcggtatt atcgacctgg tctatcccgc
    56161 acactggcag ccatacattg ctcaggatct cggtcaacaa accgatattg atatctatct
    56221 cgaccgccac gtagtgcgtc aggggcgata tctttcgctg catgatgaag taaaaaactt
    56281 tccgttacag cactggctgc gcagtacgat tatcgctgcg ggctcgctgc tagtgctgtt
    56341 tatgctgtta ttctggatcc cgctggatat gccgctgaaa ttcactctct catggatgaa
    56401 aggcgcgcag accattgaag ccaccagcgt aaaacaactg gctgacgctg gcgtgcgggt
    56461 aggcgataca ttgcgtatta gcggtacggg aatgtgtaat attcgcactt ccggaacctg
    56521 gagcgcgaaa accaattcac cttttttacc gtttgactgc tcgcagatca tctggaatga
    56581 cgcccgctca ttaccattac cagaatctga actggtcaac aaagcgacgg cattgactga
    56641 agcagttaat cgccagctgc accctaaacc ggaagatgaa tctcgcgtca gtgcctcatt
    56701 acgttcagcg attcaaaaat ccggcatggt attgcttgat gattttggcg acattgtact
    56761 gaagacagcg gatttatgtt ctgccaaaga tgactgtgtg cgactgaaaa atgcgctggt
    56821 caatctcggc aacagtaaag actgggacgc gctggtaaaa cgcgccaacg ccgggaagct
    56881 cgatggcgtg aatgtgttat tacgcccggt gagtgctgaa tcgctggata acctggtggc
    56941 aacctccacc gcgccgttta tcacgcatga aacggcgcga gcggcacaat cactaaacag
    57001 tccggccccc ggcggattcc tgattgtcag cgacgaaggc agcgattttg ttgatcagcc
    57061 ctggccttcg gcatcacttt acgactaccc gccgcaagaa cagtggaacg ctttccagaa
    57121 actggcacaa atgctgatgc atacgccgtt taacgccgaa ggtatcgtca caaaaatctt
    57181 cactgacgcc aatggtacgc agcatattgg ccttcatccg atcccggatc gttccggcct
    57241 gtggcgctat ctcagcacca cattgctgct actgacgatg ctgggtagcg ccatttacaa
    57301 tggcgtacag gcctggcgtc gttaccagcg tcatcgcact cgcatgatgg agattcaggc
    57361 ctattatgaa agctgcctga acccgcaact gatcacccct tcagaaagcc ttatcgaata
    57421 acacgtttgc gcggcaggtt atgctaccct gtcgcgcaaa ttgcttcact ctggagattt
    57481 ccctcatgca tatcaacatt gcctggcagg acgtagatac cgttctgctg gatatggacg
    57541 gcacgttgct cgacctcgcc ttcgataact atttctggca aaagctggtg cctgaaacat
    57601 ggggcgcgaa aaacggggtt acgccacagg aagcgatgga atatatgcgc cagcaatatc
    57661 acgacgtaca gcatacgcta aactggtact gtcttgatta ctggagtgag caactgggtc
    57721 tggatatctg tgcgatgacc accgagatgg gaccgcgcgc cgtactgcgt gaagatacca
    57781 ttccgtttct tgaggcactg aaagccagcg gtaagcagcg aattttgctc accaatgctc
    57841 atccgcacaa cctggcggta aaacttgagc atacgggtct ggacgcacac cttgatttat
    57901 tactttccac ccacacattt ggttatccga aagaggatca gcggttatgg catgcggtgg
    57961 ccgaagctac gggtctgaaa gctgaaagaa cgctgtttat tgatgacagc gaagcgattc
    58021 tcgatgctgc cgcgcaattt ggtattcgtt actgcctcgg cgtgactaat cctgattccg
    58081 ggattgccga gaaacagtat caacgccatc cgtcactgaa tgactaccgc cgcctgatcc
    58141 cctcgctaat gtgaaggaga cgccatgaaa gagaaacctg ctgttgaggt tcgactggat
    58201 aaatggctat gggctgcccg tttttataaa acccgcgcgc tggcccgtga aatgattgaa
    58261 ggcggtaagg tgcattacaa cgggcagcgc agcaagccga gcaaaatcgt cgagctgaat
    58321 gccacgctca ctctgcgcca gggaaatgac gaacgcacgg tgattgtaaa ggcgattact
    58381 gaacagcgtc gccccgccag cgaggcagcc ttgctgtatg aagagactgc ggaaagtgta
    58441 gagaaacgcg aaaaaatggc gatggcacgt aaacttaatg ccttaaccat gccgcacccg
    58501 gaccgacgcc cggacaaaaa agagcgccgc gacctgttac gatttaaaca cggcgacagt
    58561 gaataactgt cacctgcaag agagatgatt atgccgcaac atgaccaatt acatcgctat
    58621 ctgtttgaaa attttgccgt tcgcggcgaa ctggtaaccg tttcggaaac cctgcaacag
    58681 atccttgaga accacgatta tccgcagccc gttaaaaacg tgctggcaga actgctggtt
    58741 gcgaccagcc tgttaaccgc tacgctgaag tttgatggtg atatcaccgt acagctgcag
    58801 ggcgacggtc cgatgaatct ggcggttatt aacggtaaca ataaccagca aatgcgcggt
    58861 gtggcgcgcg tgcagggcga aattccagaa aatgccgacc tgaaaacgct ggtcggcaat
    58921 ggttacgtgg tgatcaccat taccccgagc gaaggcgaac gctatcaggg cgtagttggt
    58981 ctggaaggtg ataccctggc ggcctgccta gaagattact ttatgcgttc tgaacagctg
    59041 ccgacgcgcc tgtttattcg caccggcgac gtagacggca aaccggctgc aggcggtatg
    59101 ttgttgcagg taatgcctgc gcaaaatacc caacaggacg actttgacca cctggcgacg
    59161 ctaaccgaaa ccatcaaaac cgaagaactg ctgaccttac cggcaaacga agtgttgtgg
    59221 cgtttgtatc acgaagaaga ggtgacggtt tacgatccgc aggatgtgga gttcaaatgc
    59281 acctgctcgc gtgaacgttg cgccgatgcg ctgaaaacgc tgcctgatga agaagttgat
    59341 agcatcctgg cggaagatgg cgaaattgac atgcattgtg attactgcgg taaccactat
    59401 ctgttcaatg cgatggatat tgctgaaatc cgcaacaacg cgtctccggc agatccgtaa
    59461 gttcattaat gagtatgtcc ggcagagagg gctctgccgg attttacgac ttaatggcct
    59521 gatgtgctgc gcttaccagc gcctatgaaa taaatcgcaa attattttgg tcagatacag
    59581 cgtcgtatcc gataaaagcg ttgtcactta cttaagttta tttccagcgt ttcaccatct
    59641 accgtcagat cgcgccacgc tttcggcctc gtccccagtt ttccatcctt cgcagctgtg
    59701 agtaattttt ctttcgttat ttcgttcgga agtaaactca tatcaagcgc gtcccattct
    59761 tttctgtctg agtcaaagcc atagacaatg actctgtcat cattaaaagc aaacaggatc
    59821 cgctccgcct gaccatcact attcagatct tgctcgacca gtacgcaggc gtctttttca
    59881 atacaggtca tcacgttata gcgatcctgg attaaggccg accagaatgt cgcatccggt
    59941 ttaacagaac caggggcaat taacacattt tcggcgaata ctttttccga tacctgttcc
    60001 tgaagatgtt gctctccatc taacgccatc agcagatccc gcgcgcgttt cgggtctttc
    60061 ataaacccgg catcgctttt cagcgactca agcgccgcac gtccatagcg accgctctgt
    60121 tcgagcatgt agatagttac ctggtctggc gtgtttttgc cgctctgata acgcgccata
    60181 tgactgttca cgctaatacg catactgtcc agcaccggcg aattaagaag caccagtatc
    60241 accaacacca ataacgaaac cgcaaggttc actttgccct gaagaacaag gggattttgc
    60301 ccattacgcc agacgatact gacaaaatac cccagcgacc acaccagtaa caccagctcc
    60361 gccagcgcgc cctgcaagcg gtcgacagtc cagccgtact gagcgacccg cagccataac
    60421 ccccaggtgg cgacgaacac atacagcgga gcaaccagca aagcggtttt aatcaggcaa
    60481 cgcaacggcc ccgtccaggg aagtgacgct ttttgcggat cgcggacaat agccattaag
    60541 atcaattgca aaaaggccag cgttaacagc aacccggcgg cggagatgtg gcgagaaatc
    60601 gcgctcaggc ccgtaaacgg cagggtgatg ataaacatca gggttagcaa tgatactaac
    60661 ggcagcaacc ccgtggcgat tagcgtgaac aacttttgaa tagagtcgat taaacgtgac
    60721 tgtgtacgcg cgaggatcac cgccagtgcg gtaaccagac caaatgtaag atacataaac
    60781 cagtcggttg caaaaaagag cgtattgaaa aacgtgatgc caacaagttt aaacaactca
    60841 ccccacagta aaagcaccag ccacgtcagg ccattggaga gaaaaatcac cagtaatata
    60901 agtacgttat gccatactga ctggtaaaag tagctatagc ggctactgcc attgcggata
    60961 cgaaggcttt gttgtatcca cggtagcaac aacattgcca tcaacagcag atagcagcca
    61021 aaatcccaaa tcgccttttc agctctccag gggttcatgc catcagtttg ccacttcagc
    61081 caaccgctca tccctgtcgt ggcaataaac accagcgcca gccatcccca aaggcgcttc
    61141 tgtttaaaag agatcacgga gaagagtaaa acggatgaaa acgcgacggt cgcaggcacg
    61201 ccataaacaa tccaactgtg attttttccg gcaagccagg cgataagcag gtagcaaacc
    61261 agcccctgaa gtaatccggt agcaatcatc ccccagcgtg ttgccggtga aagttcaacg
    61321 ttatccatgt tggttatcca gaatcaaaag gtgggttaat tatcgcatcc gggcagtagt
    61381 attttgcttt tttcagaaaa taatcaaaaa aagttagcgt ggtgaatcga tactttaccg
    61441 gttgaatttg catcaatttc attcaggaat gcgattccac tcacaatatt cccgccatat
    61501 aaaccaagat ttaacctttt gagaacattt tccacaccta aaatgctatt tctgcgataa
    61561 tagcaaccgt ttcgtgacag gaatcacgga gttttttgtc aaatatgaat ttctccagat
    61621 acgtaaatct atgagccttg tcgcggttaa cacccccaaa aagactttac tattcaggca
    61681 atacatattg gctaaggagc agtgaaatgc gcgttaacaa tggtttgccc ccgcaagaac
    61741 tcgaggctta tggtatcagt gacgtacatg atatcgttta caacccaagc tacgacctgc
    61801 tgtatcagga agagctcgat ccgagcctga caggttatga gcgcggggtg ttaactaatc
    61861 tgggtgccgt tgccgtcgat accgggatct tcaccggtcg ttcaccaaaa gataagtata
    61921 tcgtccgtga cgataccact cgcgatactt tctggtgggc agacaaaggc aaaggtaaga
    61981 acgacaacaa acctctctct ccggaaacct ggcagcatct gaaaggcctg gtgaccaggc
    62041 agctttccgg caaacgtctg ttcgttgtcg acgctttctg tggtgcgaac ccggatactc
    62101 gtctttccgt ccgtttcatc accgaagtgg cctggcaggc gcattttgtc aaaaacatgt
    62161 ttattcgccc gagcgatgaa gagctggcag gtttcaaacc agactttatc gttatgaacg
    62221 gcgcgaagtg cactaacccg cagtggaaag aacagggtct gaactccgaa aacttcgtgg
    62281 cgtttaacct gaccgagcgc atgcagctga ttggcggcac ctggtacggc ggcgaaatga
    62341 agaaagggat gttctcgatg atgaactacc tgctgccgct gaaaggtatc gcttctatgc
    62401 actgctccgc caacgttggc gagaaaggcg atgttgcggt gttcttcggc ctttccggca
    62461 ccggtaaaac caccctttcc accgacccga aacgccgcct gattggcgat gacgaacacg
    62521 gctgggacga tgacggcgtg tttaatttcg aaggcggctg ctacgcaaaa actatcaagc
    62581 tgtcgaaaga agcggaacct gaaatctaca acgctatccg tcgtgatgcg ctgctggaaa
    62641 acgtcaccgt gcgtgaagat ggcactatcg actttgatga tggttcaaaa accgagaaca
    62701 cccgcgtttc ttatccaatc tatcacatcg ataacatcgt taagccggtt tccaaagcgg
    62761 gccacgcgac taaggttatc ttcctgactg ctgatgcttt cggcgtgttg ccgccggttt
    62821 ctcgcctgac tgccgatcaa acccagtatc acttcctctc tggcttcacc gccaaactgg
    62881 ccggtactga gcgtggcatc accgaaccga cgccaacctt ctccgcttgc ttcggcgcgg
    62941 cgttcctgtc gctgcacccg actcagtacg cagaagtgct ggtgaaacgt atgcaggcgg
    63001 cgggcgcgca ggcttatctg gttaacactg gctggaacgg cactggcaaa cgtatctcga
    63061 ttaaagatac ccgcgccatt atcgacgcca tcctcaacgg ttcgctggat aatgcagaaa
    63121 ccttcactct gccgatgttt aacctggcga tcccaaccga actgccgggc gtagacacga
    63181 agattctcga tccgcgtaac acctacgctt ctccggaaca gtggcaggaa aaagccgaaa
    63241 ccctggcgaa actgtttatc gacaacttcg ataaatacac cgacacccct gcgggtgccg
    63301 cgctggtagc ggctggtccg aaactgtaat gatttgaagc tggagaatat ctatccagta
    63361 tcttatagaa agcaaaacgg gaggcacctt cgcctcccgt ttatttaccc ttcttttgtc
    63421 gtgccctgcg cccgcgttac cggcactggc agccaggcgc gaatggaaag cccgccccgc
    63481 tcgctggtgc caagctccag catcccgtta tggttatcca cgatacgctg cacaattgcc
    63541 agccctaatc ccgtgccgct aatggtgcgc gcactgtcgc cgcggacaaa cggctggaac
    63601 aggtgcttac gttgttccgg cgcaattccc ggaccgtcat cttccacctg gaaccaggcg
    63661 cgattcggct ccgttccgct gctgactttg atccagccat tgccataacg ggcggcgttg
    63721 accaccatat tcgccaccgc gcgtttgatc gacagcgggt gcattttcac ttcaatgctg
    63781 ccggggtaaa gcgcggtttc aatttcccgc tcatagccac tttcggcagc aatcacctca
    63841 ccgagcactg cattaagatc cgccatttcc atcggcatct cctgcccggt gcgcaggtag
    63901 tcgataaact gctcaatgat ggcgttgcac tcttcgatat ctttattgat cgattctgcc
    63961 agatagccat cctgctcgct catcatctca gtcgccaggc gaatacgcgt cagcggcgtg
    64021 cgcaagtcgt gacttacccc cgccatcagc agcgtgcggt catccgccag ttgcttaaca
    64081 ccagccgcca tatggttaaa ggcacgggta acggaacgca cctccgaagc gccatattca
    64141 cgcagcggcg gcggaataat ccctttacca acctgcaagg ctgcgtgttc gagatcgacc
    64201 aacggtcggt tctggatacg aataaacagc cacgccccgc ctatcgccaa tagcataatc
    64261 gccagcgtat agcggaacag cggagagaaa tcgccctgat gaatttcggt cagcggcacg
    64321 cgtacccaga tattgggcga cagccaggtt ttcagccaga cgacaggcga acttttgttg
    64381 acctcaacgc gcacttccgt cgggccgccc agttgctgtg ccatctgatg gcttaagaat
    64441 tcatagtgtt gcgcccaacg cagacctgcc tcttcggcag cctcgttgga gtagagagag
    64501 atccccagct cacggtagat ctcccgacgg aaagcgggag gcacaaccaa ctgcgtgccg
    64561 tcctccagtt gcagtttgtc ggtcatcaac atacgcactt cgtacgcgag gactttatta
    64621 aactgctgga ggctcggcaa aatcgcgaag ttcagcacca ccagataagt cgtcaccagg
    64681 ctggcgaaca gcaaggtgac gatgagcaat aacgtacggg caaatgaact tcgtggcgag
    64741 aagcgcaatc gcctcatgct ttagagccgt ccggtacaaa gacgtagccc aggccccaaa
    64801 cggtctgaat gtaacgcgga tgcgctggat cttcttctac catgcggcgc agacgcgaaa
    64861 tctgcacgtc gatggagcgt tccattgcgg aatattcacg accacgggca aggttcatca
    64921 gcttatcgcg ggagagcggc tcacgcggat ggctgaccag tgccttcagt accgcaaact
    64981 caccgctggt gagcggcatc ggctcatctt cgcggaacat ttcgcgcgta ccgaggttaa
    65041 gtttgaactt accgaaagca attaccgcct cttcctgtga cggtgcgcct ggcagttcgt
    65101 tcgcctgacg acgcagcacc gcacgaatac gggccagcag ttcacgcggg ttaaacggtt
    65161 ttggaatgta gtcgtcagcg ccaatctcca ggcctacgat acggtccact tcttcccctt
    65221 tcgccgtcac cataatgatc ggcatcgggt tgctctgact acgaagacgt cggcaaatcg
    65281 acaagccatc ttcaccaggt aacattaaat ccagtaccat aagatggaaa gattcacgag
    65341 tcagcaggcg atccatctgt tctgcattag cgacgcttcg aacctggaag ccttgttcgg
    65401 tgagataacg ttccagcagc gcacgcaggc gcatgtcgtc atcgaccacc agaatcttgt
    65461 agttctcttg cattgtttgt actcccaaag gttcgcaaca atttgtaagc gtgtattctt
    65521 aaaaaagctc acgttcgtca ccagctaaat ctggtatgaa tttcagccta aattgttaca
    65581 aagcatatta aacagcagct taagtataca atttattcgg cgaaacatta ttgattctgt
    65641 tgatatgatc acgttatacc caatgtgcgc attatcaaac agacaaaggg aatcaacgag
    65701 atgaaaacgc ccctggttac ccgggaaggg tatgaaaaac tcaaacaaga gcttaattat
    65761 ctctggcgtg aagaacgccc ggaggtcaca aaaaaggtga cctgggccgc aagtctgggc
    65821 gaccgcagcg aaaatgctga ctatcagtat aataaaaagc gtctgcgtga aatcgaccgt
    65881 cgcgtgcgct atctcactaa atgcctggaa aatctcaaaa tcgtcgatta ctcccctcag
    65941 caggaaggca aagtcttttt tggcgcgtgg gtggagattg aaaacgacga tggcgtgact
    66001 caccgtttcc gtattgtcgg ctacgatgaa atttttggcc gtaaagatta catctctatc
    66061 gattccccga tggcccgcgc attgctgaaa aaagaagtcg gcgatctggc ggtggtgaat
    66121 acccctgccg gggaagcgaa ctggtatgtt aatgctatcg agtacgtgaa accgtaagga
    66181 agagtcttaa cctcctgccg atggctggca tttttgccag ccagtccgta taactatccc
    66241 ctgatttttg atccgaaaag atgaactcaa accatgatga atgattcgtt ctgccgcatt
    66301 attgcgggtg aaattcaggc gcgcccggaa caggttgacg ctgccgttcg cctgcttgac
    66361 gaagggaata ccgtgccgtt tatcgcacgt tatcgtaagg aaatcaccgg cggtctggat
    66421 gacacgcagc tgcgtaatct ggaaacgcgt ctgagctatc tgcgcgagct ggaagagaga
    66481 cgtcaggcga tcctcaaatc catttccgag caaggcaaac tcaccgatga tctggcgaag
    66541 gccatcaacg ccaccctaag caaaaccgaa ctcgaagacc tctacctgcc ctacaaacct
    66601 aaacgccgca cccgcgggca aatcgccatt gaagcagggc ttgagccgtt ggctgacctg
    66661 ctgtggagcg atccgtcaca cacgccagaa gtcgccgctg cacaatatgt tgatgccgat
    66721 aaaggcgtgg cagataccaa agccgcgctg gatggcgcgc gctatatcct gatggaacgg
    66781 tttgccgaag atgccacgct gctggcgaaa gtgcgtgatt atctgtggaa gaacgcgcat
    66841 ttggtttcta cggtggtgag cggtaaagaa gaggaagggg cgaaattccg cgactatttc
    66901 gatcatcacg aaccgttgtc cacggtgcct tctcaccgcg cgctggcgat gttccgtggg
    66961 cgtaacgaag gcgtactcca gctttcgctg aatgccgatc cgcagttcga tgagccgccc
    67021 aaagagagct attgcgagca aatcatcatg gatcaccttg gcctgcgcct gaacaatgcc
    67081 ccggcggata gctggcgcaa aagcgtagtg agctggacct ggcgcatcaa ggtgctgatg
    67141 catctggaaa ccgaactgat gggtaccgtg cgcgaacgcg cggaagatga agcaatcaac
    67201 gtctttgccc gtaacctgca cgatctgctg atggcggccc ctgccggact gcgtgcaacg
    67261 atgggcctcg atccgggtct gcgtaccggg gtaaaagtgg cggtggtcga tgccactggc
    67321 aaactggtgg cgaccgatac catttacccg cacaccggac aagccgcaaa agcagcgatg
    67381 accgttgctg ccttgtgtga aaaacataac gttgaactgg tggcgatcgg caacggtaca
    67441 gcttcccgcg aaactgaacg tttctatctc gacgtgcaga agcagttccc gaaagtgacc
    67501 gcacagaaag tgatcgtcag cgaagctggc gcgtcggttt actcagcttc cgagctggca
    67561 gcgcaggagt tcccggatct cgacgtttcg ctgcgtggtg cggtatctat cgcccgccgt
    67621 ttgcaggatc cgctggcgga gctggtgaaa atcgatccga aatctatcgg cgtaggtcag
    67681 tatcagcatg acgtcagcca gacgcaactg gcccgcaaac tggatgcagt agtagaagac
    67741 tgcgtaaacg ccgttggcgt cgatctcaac accgcttctg ttccgctatt aacccgcgtg
    67801 gcgggcctga cgcgcatgat ggcgcaaaac atcgtggcct ggcgcgatga gaacggccag
    67861 ttccagaacc gtcagcaact gctgaaagtc agccgtctgg ggccgaaagc cttcgagcag
    67921 tgcgcgggct tcctgcgcat taaccacggt gataacccgc tggacgcctc taccgttcat
    67981 ccggaagcct atccggtggt ggaacgcatt ctggcagcaa cacagcaggc actgaaagat
    68041 ctgatgggta acagcagcga actgcgtaac ctgaaagcgt ctgactttac tgatgaaaaa
    68101 ttcggtgtgc cgacagtaac tgacatcatc aaagagctgg aaaaaccggg tcgcgatccg
    68161 cgtccggaat ttaaaaccgc tcagtttgcc gatggcgtcg agacaatgaa cgacctgcaa
    68221 ccgggtatga tcctcgaagg cgcagtgacc aacgtcacca actttggcgc gtttgtcgat
    68281 attggcgtgc atcaggacgg cctggttcac atctcttcat tgtcgaacaa gtttgtggaa
    68341 gatccgcata ccgtggtgaa agcgggcgac attgtgaagg tgaaagtgct ggaagtggat
    68401 cttcagcgta aacgtatcgc cctgactatg cgtctggacg agcagcctgg cgaaaccaac
    68461 tctcgtcgcg gcggcggtaa tgaacgcccg caaaacaacc gcccggcagc caaaccacgc
    68521 ggtcgtgaag cgcagcctgc cggtaatagc gcgatgatgg atgcgctggc ggcagcgatg
    68581 ggtaaaaaac gttaaacgcc cggactggcc cctaaacctg cgcgataagg cgttcacgcc
    68641 gcatctggca acgaacgctt tgcctgacat aaaagtgccg gagaatatct ccggcacttt
    68701 tattccacag ccaaactcac aatatactcc gttaatattt atcacttcat taacaactga
    68761 aaccttaatt aaacattagc cagtccgggt aactcactat tcgaattata ttttcgctgc
    68821 gatataacct tgagccacat caacattgag tcagattatt attcaaacca acattcgcac
    68881 acattttaag tattgctgat agaaaccatt ctcattatca ttgtgttgtt gattatttaa
    68941 tctctccttt gttggcaaat catctggtct catgtcgcgg tcaaacgccc catgaggtag
    69001 ttatccagtt aatgagaaac aagtaggcac ctatgcaata cactccagat actgcgtgga
    69061 aaatcactgg cttttcccgt gaaatcagcc cggcatatcg ccaaaaactg ctttctctag
    69121 gcatgttacc tggctcctct tttgatgtgg tgcgcgtcgc tccactcggc gaccccattc
    69181 atatcgaaac ccgtcgtgtg agcctggtat tacgcaaaaa agatctggcc ttattagaag
    69241 tggaagcggt ttcctgttaa tacggtgata acaacaatga aaaaattaac cattggctta
    69301 attggtaatc caaattctgg caagacaacc ttatttaacc agctcactgg cgcacgtcag
    69361 cgtgtaggta actgggctgg cgttaccgtc gaacgtaaag aagggcaatt ctccaccacc
    69421 gatcatcagg tcacgctggt ggacctgccc ggcacctatt ctctgaccac catctcatcg
    69481 cagacctcgc tcgatgagca aatcgcctgt cactacattt tgagtggcga cgccgacctg
    69541 ctgattaacg tggtggatgc gtctaacctt gagcgtaacc tgtacctgac gctgcaactg
    69601 ctggaactcg gcattccctg catcgtggcg ctgaacatgc tcgacattgc cgagaagcaa
    69661 aatattcgta ttgaaattga tgctctatcg gcacgtcttg gctgcccggt gatcccgctg
    69721 gtttcaaccc gtggtcgcgg tattgaagcg ctcaagctgg cgattgatcg ctataaagct
    69781 aacgagaatg tggaactggt gcattacgca cagccgctac tcaacgaagc agattcactg
    69841 gcaaaagtga tgccttccga catcccgctg aaacaacgtc gctggctggg cctgcaaatg
    69901 ctggaaggcg atatctacag ccgcgcctac gccggtgaag cgtcgcagca tctggatgcc
    69961 accctcgccc gtctgcgtaa tgaaatggat gatccggcgc tgcacattgc cgatgcgcgt
    70021 taccagtgca ttgctgccat ctgtgatgtg gtaagcaaca ccctgacggc agaacccagc
    70081 cgtttcacca ctgcggtaga taaaatcgtg ctcaaccgtt tcctcggtct gccgattttc
    70141 ctctttgtga tgtacctgat gttcctgctg gctatcaaca tcggcggggc gttacagccg
    70201 ctgtttaacg tcggctccgt ggcgctattt gtgcatggta ttcaatggat tggctacacg
    70261 ctccacttcc cggactggct gactatcttc ctcgcccagg gcctgggtgg cggtattaac
    70321 accgtgctgc cactggtgcc gcagatcggc atgatgtacc tgttcctctc cttccttgag
    70381 gactccggct atatggcgcg tgcagcgttt gtgatggacc gcctgatgca ggcgctgggc
    70441 ttgccgggga aatcctttgt gccgctgatc gtcggtttcg gttgtaacgt accgtcggta
    70501 atgggcgcac gtacgcttga tgcaccgcgt gaacgtctga tgaccatcat gatggcaccg
    70561 tttatgtcct gcggcgcgcg tctggctatc ttcgcagtat tcgcggctgc cttcttcggg
    70621 cagaacggtg ccctggcggt tttctcgctg tatatgctcg gtatcgtgat ggcggtgctg
    70681 actggcctga tgctcaagta caccatcatg cgcggtgaag cgacgccgtt tgtcatggag
    70741 ctgccggtct accatgtacc acacgttaaa agcctgatta tccagacctg gcagcgtctg
    70801 aagggcttcg ttctgcgtgc tggtaaagtg attatcatcg tcagcatttt cctgagcgct
    70861 ttcaacagct tctcgttgag cgggaaaatc gtcgataaca tcaacgactc ggcgctggct
    70921 tccgtcagcc gggtgataac ccctgtcttc aagccgattg gcgtacatga agataactgg
    70981 caggcaacgg ttggcctgtt tacgggcgca atggcaaaag aagtggtggt gggtacgctc
    71041 aacaccctct acaccgcaga aaatattcag gacgaagagt tcaatccggc ggaattcaac
    71101 ctcggtgaag agctgttcag tgcggtagat gaaacctggc agagcctgaa agacaccttc
    71161 agccttagcg tactgatgaa ccccattgaa gctagcaaag gcaacggtga aatgggtacc
    71221 ggggcgatgg gcgtgatgga tcagaaattc ggtagcgcag ctgccgcata cagctacctg
    71281 attttcgtcc tgctgtatgt accatgtatc tcggtgatgg gagccatcgc ccgtgaatca
    71341 agccgtggct ggatgggctt ctccatcctg tgggggctga atatcgctta ctcactggca
    71401 acattgttct atcaggtcgc cagctacagt cagcacccaa cttacagcct ggtgtgcatt
    71461 ctggcggtaa tcctgtttaa catcgtagct atcggtctgc tgcgccgcgc gcgtagccgg
    71521 gtggatatcg aactgctggc aacccgcaag tcggtaagca gttgctgcgc agccagcacc
    71581 accggtgatt gccattaatg gcttcactta ttcaggtacg tgatttgctg gcgttacggg
    71641 gccgtatgga agcgacccag ataagccaga cattgaacac tccgcagcca atgattaacg
    71701 ccatgctgca acagctggaa agcatgggca aagccgtgcg gattcaggaa gaacctgacg
    71761 gctgcctctc tggcagttgt aaaagctgcc cggaaggcaa agcctgtctg cgcgagtggt
    71821 gggcgctgcg ttaaccatac tccatcgcct gttttgaaaa gcccggtatg cgtttgcatc
    71881 cgggcttttt tgcgtgcggc tttccataaa aatgcaattc ttgcagcgca acgtaagttc
    71941 ctctgaaagc gtctcgcagg gatgaaaact cgctaatgca cagatgtgga gtggcgcgta
    72001 gagtcgcggc attcaaacaa caggtgaagg aacgccatga gtaaaaagca gagttccacc
    72061 ccacatgatg cgctgttcaa actcttttta cgccaaccgg agacggcacg cgattttctt
    72121 gcgtttcatt taccggcacc cattcacgcg ctctgtgata tgaaaaccct caagctggag
    72181 tcgaggagct ttattgatga cgatctgcgt gaaagctatt ccgatgtgct gtggtcggtg
    72241 aaaacggaac aaggaccagg atacatctat tgtctgattg aacatcaaag cacctcaaac
    72301 aaactgatcg catttcgcat gatgcgttac gctattgccg caatgcaaaa tcaccttgat
    72361 gctggataca aaacgttgcc gatggtggtg ccattgttgt tttaccacgg tattgaaagc
    72421 ccctatccct attcgctgtg ttggctggat tgtttcgccg atcccaaact ggcaaggcag
    72481 ctttatgcct ccgcatttcc gctgattgat gtcaccgtca tgcctgatga tgaaatcatg
    72541 cagcaccgac gtatggcgct gctggagtta attcaaaaac atattcgtca acgcgatctg
    72601 atggggctgg tagagcaaat ggcctgctta ttaagtagtg gatacgctaa tgacagacaa
    72661 atcaaagggc tgtttaatta catactgcaa actggcgacg ctgtacgttt taacgatttt
    72721 atcgacggcg ttgccgaacg ttcaccgaaa cacaaggaga gtttaatgac tattgcggaa
    72781 agattgcggc aggaggggga acaatccaaa gccctgcata tagccaaaat aatgcttgaa
    72841 tccggagtcc ctcttgcaga catcatgcga tttaccgggc tgtcagaaga agagttggct
    72901 gcgg
//
LOCUS       DAJTSF010000005        72074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000005 DAJTSF010000000
VERSION     DAJTSF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.005"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1368..2954)
                     /locus_tag="OO335_000387"
     CDS             complement(1368..2954)
                     /locus_tag="OO335_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR6000902.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(3309..5480)
                     /gene="glcB"
                     /locus_tag="OO335_000388"
     CDS             complement(3309..5480)
                     /gene="glcB"
                     /locus_tag="OO335_000388"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR6000903.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5502..5906)
                     /locus_tag="OO335_000389"
     CDS             complement(5502..5906)
                     /locus_tag="OO335_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR6000904.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5911..7130)
                     /gene="glcF"
                     /locus_tag="OO335_000390"
                     /pseudo
     CDS             complement(5911..7130)
                     /gene="glcF"
                     /locus_tag="OO335_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(7141..8193)
                     /gene="glcE"
                     /locus_tag="OO335_000391"
     CDS             complement(7141..8193)
                     /gene="glcE"
                     /locus_tag="OO335_000391"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR6000905.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8193..9692)
                     /gene="glcD"
                     /locus_tag="OO335_000392"
                     /pseudo
     CDS             complement(8193..9692)
                     /gene="glcD"
                     /locus_tag="OO335_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9943..10713
                     /gene="glcC"
                     /locus_tag="OO335_000393"
     CDS             9943..10713
                     /gene="glcC"
                     /locus_tag="OO335_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR6000906.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10837..11933)
                     /locus_tag="OO335_000394"
                     /pseudo
     CDS             complement(10837..11933)
                     /locus_tag="OO335_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12295..14025
                     /locus_tag="OO335_000395"
     CDS             12295..14025
                     /locus_tag="OO335_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR6000907.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            14022..14936
                     /locus_tag="OO335_000396"
     CDS             14022..14936
                     /locus_tag="OO335_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR6000908.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14969..15217
                     /locus_tag="OO335_000397"
     CDS             14969..15217
                     /locus_tag="OO335_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6000909.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            15217..16414
                     /locus_tag="OO335_000398"
                     /pseudo
     CDS             15217..16414
                     /locus_tag="OO335_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(16450..17529)
                     /gene="lptG"
                     /locus_tag="OO335_000399"
     CDS             complement(16450..17529)
                     /gene="lptG"
                     /locus_tag="OO335_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6000910.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17526..18596)
                     /gene="lptF"
                     /locus_tag="OO335_000400"
     CDS             complement(17526..18596)
                     /gene="lptF"
                     /locus_tag="OO335_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6000911.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18627..19187)
                     /locus_tag="OO335_000401"
     CDS             complement(18627..19187)
                     /locus_tag="OO335_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6000912.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19199..20023)
                     /locus_tag="OO335_000402"
     CDS             complement(19199..20023)
                     /locus_tag="OO335_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000913.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20023..21372)
                     /locus_tag="OO335_000403"
     CDS             complement(20023..21372)
                     /locus_tag="OO335_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR6000914.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21418..22176)
                     /locus_tag="OO335_000404"
     CDS             complement(21418..22176)
                     /locus_tag="OO335_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000915.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22208..22921)
                     /locus_tag="OO335_000405"
     CDS             complement(22208..22921)
                     /locus_tag="OO335_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6000916.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23095..23787
                     /locus_tag="OO335_000406"
     CDS             23095..23787
                     /locus_tag="OO335_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6000917.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23836..25335)
                     /gene="pitB"
                     /locus_tag="OO335_000407"
     CDS             complement(23836..25335)
                     /gene="pitB"
                     /locus_tag="OO335_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR6000918.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25627..27486)
                     /gene="gss"
                     /locus_tag="OO335_000408"
     CDS             complement(25627..27486)
                     /gene="gss"
                     /locus_tag="OO335_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6000919.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27691..28557
                     /gene="yghU"
                     /locus_tag="OO335_000409"
     CDS             27691..28557
                     /gene="yghU"
                     /locus_tag="OO335_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6000920.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28680..28928)
                     /gene="hybG"
                     /locus_tag="OO335_000410"
     CDS             complement(28680..28928)
                     /gene="hybG"
                     /locus_tag="OO335_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6000921.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29062..29403)
                     /gene="hypA"
                     /locus_tag="OO335_000411"
     CDS             complement(29062..29403)
                     /gene="hypA"
                     /locus_tag="OO335_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6000922.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29396..29884)
                     /gene="hybE"
                     /locus_tag="OO335_000412"
     CDS             complement(29396..29884)
                     /gene="hybE"
                     /locus_tag="OO335_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6000923.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29877..30371)
                     /locus_tag="OO335_000413"
     CDS             complement(29877..30371)
                     /locus_tag="OO335_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6000924.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30371..32074)
                     /gene="hybC"
                     /locus_tag="OO335_000414"
     CDS             complement(30371..32074)
                     /gene="hybC"
                     /locus_tag="OO335_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6000925.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32071..33249)
                     /gene="hybB"
                     /locus_tag="OO335_000415"
     CDS             complement(32071..33249)
                     /gene="hybB"
                     /locus_tag="OO335_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6000926.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33239..34225)
                     /gene="hybA"
                     /locus_tag="OO335_000416"
     CDS             complement(33239..34225)
                     /gene="hybA"
                     /locus_tag="OO335_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6000927.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34228..35346)
                     /gene="hybO"
                     /locus_tag="OO335_000417"
     CDS             complement(34228..35346)
                     /gene="hybO"
                     /locus_tag="OO335_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6000928.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35535..35822)
                     /locus_tag="OO335_000418"
     CDS             complement(35535..35822)
                     /locus_tag="OO335_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6000929.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35941..36828)
                     /locus_tag="OO335_000419"
     CDS             complement(35941..36828)
                     /locus_tag="OO335_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6000930.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36985..38025
                     /gene="gpr"
                     /locus_tag="OO335_000420"
     CDS             36985..38025
                     /gene="gpr"
                     /locus_tag="OO335_000420"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6000931.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38065..38559)
                     /locus_tag="OO335_000421"
     CDS             complement(38065..38559)
                     /locus_tag="OO335_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6000932.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38750..39634
                     /gene="yghA"
                     /locus_tag="OO335_000422"
     CDS             38750..39634
                     /gene="yghA"
                     /locus_tag="OO335_000422"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6000933.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39906..40331)
                     /gene="exbD"
                     /locus_tag="OO335_000423"
     CDS             complement(39906..40331)
                     /gene="exbD"
                     /locus_tag="OO335_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6000934.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40338..41072)
                     /gene="exbB"
                     /locus_tag="OO335_000424"
     CDS             complement(40338..41072)
                     /gene="exbB"
                     /locus_tag="OO335_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6000935.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41324..42511
                     /gene="metC"
                     /locus_tag="OO335_000425"
     CDS             41324..42511
                     /gene="metC"
                     /locus_tag="OO335_000425"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6000936.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42651..43310
                     /locus_tag="OO335_000426"
     CDS             42651..43310
                     /locus_tag="OO335_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6000937.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43350..44249)
                     /gene="yqhC"
                     /locus_tag="OO335_000427"
     CDS             complement(43350..44249)
                     /gene="yqhC"
                     /locus_tag="OO335_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6000938.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            44443..45606
                     /gene="yqhD"
                     /locus_tag="OO335_000428"
     CDS             44443..45606
                     /gene="yqhD"
                     /locus_tag="OO335_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6000939.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45711..46538
                     /gene="dkgA"
                     /locus_tag="OO335_000429"
     CDS             45711..46538
                     /gene="dkgA"
                     /locus_tag="OO335_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6000940.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46738..47664
                     /locus_tag="OO335_000430"
     CDS             46738..47664
                     /locus_tag="OO335_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6000941.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYLQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47715..47972
                     /gene="yqhH"
                     /locus_tag="OO335_000431"
     CDS             47715..47972
                     /gene="yqhH"
                     /locus_tag="OO335_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6000942.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(48015..50234)
                     /locus_tag="OO335_000432"
     CDS             complement(48015..50234)
                     /locus_tag="OO335_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR6000943.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(50345..51757)
                     /gene="ftsP"
                     /locus_tag="OO335_000433"
     CDS             complement(50345..51757)
                     /gene="ftsP"
                     /locus_tag="OO335_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6000944.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51832..52569)
                     /gene="plsC"
                     /locus_tag="OO335_000434"
                     /gene_synonym="parF"
     CDS             complement(51832..52569)
                     /gene="plsC"
                     /locus_tag="OO335_000434"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6000945.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52803..55061)
                     /gene="parC"
                     /locus_tag="OO335_000435"
     CDS             complement(52803..55061)
                     /gene="parC"
                     /locus_tag="OO335_000435"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6000946.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55607..56089)
                     /locus_tag="OO335_000436"
     CDS             complement(55607..56089)
                     /locus_tag="OO335_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR6000947.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(56142..56534)
                     /gene="ygiW"
                     /locus_tag="OO335_000437"
     CDS             complement(56142..56534)
                     /gene="ygiW"
                     /locus_tag="OO335_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6000948.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56686..57345
                     /gene="qseB"
                     /locus_tag="OO335_000438"
     CDS             56686..57345
                     /gene="qseB"
                     /locus_tag="OO335_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6000949.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELLHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            57342..58616
                     /gene="qseC"
                     /locus_tag="OO335_000439"
     CDS             57342..58616
                     /gene="qseC"
                     /locus_tag="OO335_000439"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6000950.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58662..58994)
                     /locus_tag="OO335_000440"
     CDS             complement(58662..58994)
                     /locus_tag="OO335_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR6000951.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            59313..59894
                     /gene="mdaB"
                     /locus_tag="OO335_000441"
     CDS             59313..59894
                     /gene="mdaB"
                     /locus_tag="OO335_000441"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6000952.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59925..60239
                     /locus_tag="OO335_000442"
     CDS             59925..60239
                     /locus_tag="OO335_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6000953.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(60287..62179)
                     /gene="parE"
                     /locus_tag="OO335_000443"
     CDS             complement(60287..62179)
                     /gene="parE"
                     /locus_tag="OO335_000443"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6000954.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(62208..62789)
                     /gene="yqiA"
                     /locus_tag="OO335_000444"
     CDS             complement(62208..62789)
                     /gene="yqiA"
                     /locus_tag="OO335_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6000955.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62789..63616)
                     /gene="cpdA"
                     /locus_tag="OO335_000445"
     CDS             complement(62789..63616)
                     /gene="cpdA"
                     /locus_tag="OO335_000445"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6000956.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63641..64063)
                     /locus_tag="OO335_000446"
     CDS             complement(63641..64063)
                     /locus_tag="OO335_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR6000957.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(64064..64693)
                     /gene="nudF"
                     /locus_tag="OO335_000447"
     CDS             complement(64064..64693)
                     /gene="nudF"
                     /locus_tag="OO335_000447"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6000958.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64898..66379
                     /gene="tolC"
                     /locus_tag="OO335_000448"
     CDS             64898..66379
                     /gene="tolC"
                     /locus_tag="OO335_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6000959.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            66527..67198
                     /locus_tag="OO335_000449"
     CDS             66527..67198
                     /locus_tag="OO335_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6000960.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            67204..68364
                     /locus_tag="OO335_000450"
     CDS             67204..68364
                     /locus_tag="OO335_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6000961.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(68402..69190)
                     /gene="ygiD"
                     /locus_tag="OO335_000451"
     CDS             complement(68402..69190)
                     /gene="ygiD"
                     /locus_tag="OO335_000451"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6000962.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            69333..70106
                     /gene="zupT"
                     /locus_tag="OO335_000452"
     CDS             69333..70106
                     /gene="zupT"
                     /locus_tag="OO335_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6000963.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(70164..70334)
                     /locus_tag="OO335_000453"
     CDS             complement(70164..70334)
                     /locus_tag="OO335_000453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000964.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70595..71248)
                     /gene="ribB"
                     /locus_tag="OO335_000454"
     CDS             complement(70595..71248)
                     /gene="ribB"
                     /locus_tag="OO335_000454"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6000965.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(71352..71500)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71622..71912
                     /gene="ubiK"
                     /locus_tag="OO335_000455"
     CDS             71622..71912
                     /gene="ubiK"
                     /locus_tag="OO335_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR6000966.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        18032 a        19151 c        17736 g        17155 t
ORIGIN      
        1 cgtaccgttt aaacaaagac aatacgtctc tttatttctc ataaaacccc aatcccttac
       61 agggcttatt gaaaaattta aaaatcacaa atttctcaat actcgaaatg agatcacata
      121 agaaatcaaa aagccttctc atctatttta agttttccca acggaaaata agagctttca
      181 ttaatactga accactttac tgttctattt gttcaggtat tgcattacgg tgtgctgaga
      241 tccatcggtg catccctgta tcccgcgatg agactaaaat agtcgtgatg taagctcgta
      301 tcagattctt agtgaatacc acatttgtta taattaagtg aaaagataca ttgctgattt
      361 acattatttg gtttacaaaa tgtcatcact tgcagagtta tcgctgacag atacctttaa
      421 tctcttacta ctttcgtgtt attgcaatac aaccatccgt aaagcagggc tatttttagc
      481 aggaaatact caggtgtcaa cattcgaact ccaactctga aatattcata taagttaatg
      541 attttaatga ttttaatgat tttaatgatt ttaatgattt taatgatttt aatgatttta
      601 atgattttaa tgattttttc atttattcac acctaataaa agtattcaaa ttcagatgat
      661 ttttctgctc aacagacaca aacaggcaat aatactctcc aaagcgaaca atatttggca
      721 ttacgattta atgcttcata atcaccaaat tatttaacca taaattttct tatttctaac
      781 aaaatgattg gtgtgttgtt ggagttaaaa tacatacata atattaacat ctgccaacat
      841 ttcactttcc gcaagacgga tgatttcgaa tcctcatcca cgttacccta tcccaaatat
      901 ctttattaat aggcagtaac gaacactgtt atagcaagat taacttatta acctaacggc
      961 atcttttatg taacaagtgg agataaattg cttaaaattg atcctcgaca aatcttaaaa
     1021 gttctcatta ttctgcaaaa tcagctttgc gaataatctt atgtttttgg gagttttggg
     1081 aattttgatc tactcattgt tctagtgaaa aatataaata agagaaaaaa cgccattcgt
     1141 catgaaagca acagatgaag caatgtaata acatactaaa attgatctca tgaaagtggg
     1201 tactttataa ccagcacaga agccaagttg tataaatatt aaacaattcg catcgtaaaa
     1261 cggagactac gtataaagaa gccaatcgta aaaacggttc cctggcgaga taaacgacag
     1321 acaaatggaa agccgggtga tatttacctt catccggctt tattgattta cgagactaac
     1381 atcccggtaa acacatacgc ctgcagcagg gtgataacgc tggcaaaaat cagactgtgc
     1441 ttcacggtgt agcggaacag ttcagattct cggcccacca tgcccgtcgc ggcgcaggcc
     1501 acggcgatag attgcgggga gatcatcttg ccagttacgc cgccgctggt atttgctgcc
     1561 accagcagag tgtcagagac gttgatttgc tgcgccgtgg tcgattgcag tgaaccaaac
     1621 agggcgtttg aagaagtgtc cgacccggta aggaatacgc ccagccagcc gagaaacggt
     1681 gagaaaaacg ggaacatcac gcctgtacct gccagtacca gcgccagcgt ggtggacatg
     1741 ccggaatagt tggtgacgaa ggcgaatgcc agcaccatgc caatcgacaa tatcggccac
     1801 ttcagactaa ttagcatttc ggcaaagacg ccaatccctt tcttgatgcc cacgccgagg
     1861 atgaagatag agataatcgc cgcaataaaa atagcggtgc cgccagccga gagagggtcg
     1921 aatttaaaca ccgcatccat tggcgttggt tgggcgacaa tgggtgccgc tttcaacact
     1981 tgttgatgca aatgagggat ctggaaatta atcaccagtg aataaaacgc gccgcccgga
     2041 gcaaataacg ctttaaacgg cttcatggtc cagatggtga ccagcaccgt taagattaaa
     2101 aacggtgacc acgcacgaat gatttgcccc agactatatt ctgaaggcac gggaccgcca
     2161 gaagatggct tatttaccac catcgcacct gcggattgcc ccatgctgat tgccgtttcg
     2221 gtatttttcg gccgccagac tttaaggaat aaagcgagtg agacgatact caccagcgcc
     2281 gaagtaatat ccggcagttc cggaccaata tagttagagg taaagaactg agtgacagcg
     2341 aagcttcccc cagcaaccag cgccgctggc cacgtctctt tcaccccttt ccagccgtcc
     2401 atcattgcta ccagccagaa cggcacaaga accgacagga acggtaactg acgtcccgcc
     2461 attgcgccaa tgtggaacgg atcgattccc gttacctgac cggcgaccag aatcggcacg
     2521 cccaacgcac caaacgccac cgtcgcacca aagccagcag ctccttccag caacgcacca
     2581 aaggagaaac caatcagtaa cacctgcaaa cgctgatcgt cggtgatgga gataaccgag
     2641 ctgcggataa tatcgaactg cccgctggca acggttaatt tatacaggaa caccgccgcg
     2701 acaataatcc acgcaattgg ccataatcca taaataaagc catagcccgc agcagcaaat
     2761 gccatatcaa tcggcatttt aaaggcgaat atcgcaatca ggatagataa tataagggtt
     2821 attgctccag caacatgtcc tttcagacgt aataccgcga gtgcaacgaa gaagaatatt
     2881 atcgggatca gggcgaccag agcggatagc cccagtcctc ccatcggcat atacatttgg
     2941 gtccaggtaa ccatattgtt cagtctctta ttatctttat atgcttgata tacttaaggt
     3001 tgtaataagc aaaagaggac tgaactgtaa aatataggcg ttatacttta cagcaacagt
     3061 acgccgctaa cgcaattgct acctctggca taacaagtat atcgggtaag ggtttctgtt
     3121 ccgcacacgc aggcgcagag tatcgttaag atgtccatat tgttgtttta ggcccgctag
     3181 taatgcgcta cgggtattta atattgttaa accctgataa tcgctccggt tatttccggg
     3241 ataaatgtac taccgcagtt actatcatag ccccgacaat aaaacttgcc ggggcttttt
     3301 tgacgctatt aatgactttc tttttcgcgt aaacgccagg cgtgtaataa cggttcggta
     3361 taaccgtttg gctgtttcac gccgaggaag attaaatcgc tggcagcttt aaaagcacac
     3421 gagttagcga aattccccgc catcggacga taagccggat cgccagcatt ttgctgatca
     3481 accactttcg ccatattctc cagcgacgcc tgcacctgtt ctttggtcag aataccgtga
     3541 cgtaaccagt tggcgatatg ctggctggag atacgcagcg ttgcgcggtc ttccatcagc
     3601 gccacgttgt gaatatccgg cacttttgaa caaccaatcc cctgctccac ccaacgcacc
     3661 acgtacccca gaatcccctg cacgttgtta tccagctctt gttggatctc ttgcgccgac
     3721 cagttagcgt tttcagccac cggaatagtc agcagatcgt ccagcagcgg ttcaaattca
     3781 gcactgaact cggtctgggc aatgttggct tgcacgctct gtacgttggt atggtggtag
     3841 tgcagcgcat ggagcgtagc agcggttggt gacggaaccc aggctgtgtt tgccccggca
     3901 cgcagttggt cgcccttctg gctgtacatg tctgccatca ggtccggcat tgcccacatg
     3961 cctttaccga tttgcgcttt accgcgcagc ccacagaaca gaccggaaag cacgttatta
     4021 cgttcgtagg ctttgatcca cggcgtcgat ttcatctggt ttttacgcag catcgggcca
     4081 gcttccatca ccgaatgcat ttcatcgccg gtacggtcga ggaaaccggt attgatgaac
     4141 gccacgcggt tgcgcgcctg agcgatacag ctacgcaagt tcagcgaggt ccggcgttct
     4201 tcatccataa tgcccatttt cagggtattc ggtgccatac cgagcattgt ctcaatgcgg
     4261 gtaaacagtt tgttggcgaa cgccacttcc tgcggaccgt gcattttcgg tttcacaata
     4321 tagacgctgc cagtgcgcga gtttttctgc acttttaaat catagagggc aatcgcgcca
     4381 gtcatgacgc catcaagaat accttccggg atttcattgc cttcgctgtc ccaaatcaca
     4441 ggaatggtca tcaaatgacc cacgttgcgg ataaacagca acgagcgtcc gtgcagagaa
     4501 atttcagagc catcggcggc ggtgtactga cgatcgtcat tcagtttacg tacgatttgc
     4561 cgaccgtttt tctccatttt ctcttgcaga gtcccctgca tcaggcccag cagattgcgg
     4621 tacagcagga ttttatcttc cgcatcaacc gccgcgaccg aatcttcgca gtcgagaatg
     4681 gtactgatag cagcttcgac gataacatcg ttgatgtgcg ccggatcgtc tttgccaatc
     4741 cgcccgttgg catcaatttg cagttcaata tgcaggccgt tatttttcag caaaatgcag
     4801 gtcggcgcag cggcatcgcc acggtaaccg acaaactgtg ctggagtacg taacgtggtt
     4861 tctttaccat ttttcaactg gatgcgtaat tgtttatcga ccaccttaaa cgccaccaca
     4921 tcctgatagc tgccgttttc cagcggtagt gattcatcga ggaaacgtcg aacccaggcg
     4981 ataacctgct caccgcgctg cggatcgtag ccactgacca tcgccccttc ctgcgggatg
     5041 atgtcgctgc cgtataacgc atcgtacagt gagccccagc gagcgttcgc cgcgttcagc
     5101 gcgtagcggg cgttcattgc cggaaccacc agctgtggcc ccgcctgact ggtgatttcg
     5161 ctgtcaatgc ccgtggtttc caccgtcacg cgctccggtt gcggcaccag gtagcccagt
     5221 tcacgaagga aagatttata ggccgcttta tcttttaccg gccccggatt gctgcgatgc
     5281 cactcatcaa gcgccgcctg aatgcgatcg cgttctgcca gcaactgacg attttctggt
     5341 gccagatcgt gaacgatctc atcaacattg tgccagaatg cagcagcgtc cagttccacg
     5401 cccggcaaaa cttctccatc cacaaaacgt ttaaaattgg cgtcaatgcg taaacggccc
     5461 tgggttatgg tttgactcat tgtttctctc ctcgttttcg cttatttcgc caacaccgct
     5521 gctgccactt tcgcgacctg cgcatcctgt gctccggtta aaccagaaac gcccacggca
     5581 ccaataattt gcccatcaac aacaaccggt acgccgcctt ccagcgacgt cagtaacggc
     5641 gcagtcacga acgcggtacg tccgttgttc accatctctt catagccctt agtttcacga
     5701 cgccccagcg cggcggtacg cgctttctcc tgggagatgt aagccgcaat cggcgcgcaa
     5761 tcgtccatgc gacttaacgc cagcagatga ccgccgtcat cggcaacagc aatggaaaca
     5821 gaccagttat ttttctgcgc ctcttcctga cctgcggcaa taattgcact cgccatttgc
     5881 tggctaagaa tgactttagt tttcattttt ttattccttt tcaagggctt gttctacaat
     5941 ttcaatccag tgacgcacag aggtacgacc ggcgctcgcc agatgcgtct ggcaaccaat
     6001 gttggcggtg acgatcattt ccggtttgcc gctttccagc gcattcattt tgttatcccg
     6061 cagctggcgt gccagatcgg gatgcgttaa cgcatatgtt cccgctgaac cgcagcacag
     6121 atggctgtcg ggaacgtccg ttaaggtaaa tccaagacga agtaacactt tttccacttc
     6181 gccgttcagc ttttgcgcat gttgtagggt acacgggcag tggaaggcca gttttttatc
     6241 gccgcgaatt gccagttttt ccagcggttc ctcgcgcaga agttcgacta aatcgaccgc
     6301 cagttcactg acctgacgcg ctttatcggc atataacgca tcgtttttca gcatctgccc
     6361 atactctttg acgaacgcgc cgcaaccgct ggcggtttgc aaaattgcct cggcacctgc
     6421 ttcaatcgcg ggccaccagg catcaatatt attgcgcgcc cgtgccaacc ctttctcctg
     6481 cgcattaaga tgatagtcca ccgcgccaca acagcctgct tcgttagctg acatgacgct
     6541 gatccccaga cgatccagca ctcgcgcagt tgccgcgttg gtgttgggcg aaagcgtagg
     6601 ctgggcgcag ccttccagca ttaaaacccg acgcttatgg cgcagcggcg gacgcggttt
     6661 agctttcacc gtttcagcag gcagttttgc tctgacctgt tccggtaaaa acggtcgcag
     6721 caccagccct acctgcgtca gcgcacggaa gaccgccgga cgcggcacta cctggcgcaa
     6781 tccttcgcgc agcattcgct ccggcagtgg gcgtttcact ttctgctcga caatatcacg
     6841 cccgatatcc agcaaattgt gatagcgcac accagaagga caggtggttt cacaattgcg
     6901 gcaagtgagg cagcgatcga gatgctcctg tgttttaagc gtgacttcgt tgccttccag
     6961 cacctgttta atcagataga tgcgcccgcg cggcccgtcc agttcatcgc ccagaagctg
     7021 ataggttggg caggttgcgg tacaaaatcc gcagtgaaca caggcgcaca ggatgctgtc
     7081 ggcttccagc gcgcgttctg ccgcatctct tcggttaatc gggtttgcat agcctgctcc
     7141 tcaaagttcc gcgtacatgc gaccggggtt aaacacgccg caagggtcga gctgctgttt
     7201 aagctgctgg tgatagcgga ataaaggagc cgatagcggg gcaaagccac catctccggc
     7261 actaaagcgg gtcgcatgac cgccagcgtt gcgggcgatg cgatggattt gattgtcatc
     7321 ggctgtcgat ttcagccagc gtaacgcccc gccccagtcg atcagttgct cgccgggtaa
     7381 atccatcatc ggcgcatcgc tgggtaatga aatgcgccat aaggtacctg gtaacgagaa
     7441 gaacggcagt tgttgttcac gcaattgctg ccagaactga cccacaacct cttcgccacc
     7501 cagcagttca cgcgctgctt ttaccgatcc ttcgccgccc tcaaggcgga tccacaacgc
     7561 attgtcgaag taacataagc cactaatggg taatggctgg agttgccact cggcgatttc
     7621 actcatggct tcttgcaggc tgatttcccg acgcaggctc agagaggcac gcggtcgcgg
     7681 taacactttc attgagattt cagtgagcac gccaagacaa ccgtagcttc cggccattaa
     7741 ccgtgagaga tcgtatccgg caacgttttt catcacttcg ccaccaaaac gcagatgttt
     7801 tccagtgccg gtaatgatgc gcgtgccgag gacaaaatcg cggaccgaac cgctccacgg
     7861 gcgacgcggc cccgccagcc cgcaggcgac catcccgccc caggtggctt cttcaccata
     7921 atgcggcggc tcgcagggaa gcatttgccc ggcgctctcc agcgccgctt caatcgccac
     7981 cagcggcgtt ccggcacgag cggttatcac cagctcggtc gggtcgtaat taacaatgcc
     8041 gcgatgacaa cgcacatcca gcgtttgccc ggtgactggg cgacctaaaa aggctttgct
     8101 attgctgccc tgaatcacca gcggcgtttt atcgctaatt gcctgattca cctgctccag
     8161 cagcgcctga ctgtaatcac actcgcgtag catcaaaagc gctccagttc aggaaaaggt
     8221 aaatgaccgt gatgcacatg catggcacca aattcagcac agcggtgtag cgtgggaatg
     8281 tttttcccag ggttcagcaa accatcgggg tcaaacgccg ccttgaccgc atggaaggtc
     8341 gtgatttcat cgctgttgaa ctgggcgcac atttgattga ttttttctcg cccgatgcca
     8401 tgttccccac tgatgctgcc gccaacttca acgcagagtt cgaggatctt cccgcccagc
     8461 tcttccgcgc gggcaaattc accaggttcg ttggcatcga aaaggattaa cgggtgcatg
     8521 ttgccatctc cggcatgaaa gacgttggca acacgtaaat catattgctg cgataaacgg
     8581 gcaatgcctt ccagtacgcc aggcagggcg cgacgcggga tggtgccatc catgcagtag
     8641 taatccgggg agatacgtcc taccgccggg aacgcatttt tgcgaccggc ccagaaacgt
     8701 acgcgctctg cttcgtcctg tgccaaacgg acgtcagtcg cgcccgcttt caacaagatg
     8761 tagttaaccc gctcgcagtc ttcctgtacg tcagactcca cgccgtccag ttcgcataac
     8821 aaaatcgctt cggcgtcgac gggataaccg gcatgaataa aatcttccgc cgcgcggatc
     8881 gacaggttat ccatcatctc cagcccgccg gggataatgc cattggcgat gatgtcacca
     8941 accgcaagtc cggctttttc taccgagtca aagctggcta acagaacccg cgccacgggc
     9001 ggcttcggca gcagttttac cgtcacttcg gtggtcacgc cgagcatacc ttccgatccg
     9061 gtgaacagcg ccagcaggtc aaaaccaggt gaatccagcg cgtccgatcc cagcgtcagc
     9121 gcctcgccgt ccagcgtttg cacttcaatt ttcagcaggt tatgtacggt cagaccatat
     9181 ttcaggcagt ggacgccacc ggcattttcc gccacattgc cgccaattga acaggcgatt
     9241 tgtgaggaag gatccggcgc gtaatagaga ttatgcggcg caacggccta ggagatcgcc
     9301 aggttacata cgcccggctg cacgcgcgcg cggcgaccaa cggggttaat gtcgaggctc
     9361 tctttaaagc gcgccatcac caacaacaca cctttttcca gcggcagcgc gccaccagaa
     9421 agcccggtgc ctgcaccacg ggtcaccacc ggtacacgca ggcgatggca gacagccaga
     9481 atcgctgtca cctgttccat ttgcttaggc agaacaacca gtaatggacg cgtgcgatac
     9541 gcgctcaacc cgtcacactc gtaaggaatg atctcctcat cggtatgcag gatctcaagt
     9601 ccagggacat gctcacgcag tgccatcagt accgatgtgc ggtcgacatc gggtaaagcg
     9661 ccatcaagac gctcttcgta caagatgctc atgagtaggc ttcgctttgt tgtgttgtgt
     9721 ggcagctgat ttttgcgcgc tgcttctgtg aacagttatt aagcgggctt ttcgttttcg
     9781 tctatctctt tagctaccgg tcagaccatt ttttttccag ctctgtgacc ttgtcttggt
     9841 taacttaatg ttaaattgat gtaacataat cacttgcgtg atgtgcgtgt tttgcgagtt
     9901 aagaacagaa aaattggtcc tacctgtgca cgaggtccgg gaatgaaaga tgaacgtcgc
     9961 cctatttgcg aagtggttgc agagagtatc gaacggttaa ttatcgacgg cgtactgaag
    10021 gtcggtcagc cgcttccctc ggaacgtcga ctgtgtgaaa agctcggctt ctcacgctcc
    10081 gcactgcgtg aagggctgac cgtgctgcgc gggcgcggga ttattgaaac ggcgcagggt
    10141 cgcgattctc atgtcgcacg gcttaatcgg gagcaggaca ccagcccgct gatccatctg
    10201 ttcagtacgc agccgcgaac gctgtacgat ctgctcgacg ttcgcgcatt actggagggc
    10261 gaatcggcaa ggctggcggc aacgctggga acgcaggctg attttgttgt gataacccgc
    10321 tgttatgaaa aaatgctcgc cgccagtgag aaccacaaag agatttcgct gatcgaacat
    10381 gcgcagttgg atcacgcttt ccatctcgcc atttgtcagg cttctcacaa tcaggtgctg
    10441 gtgtttacgc tgcaatcatt gaccgatctg atgtttaatt cagtgtttgc cagcgtaaat
    10501 aatctctacc atcgaccaca gcaaaaaaag cagatcgatc gccagcatgc gcggatctac
    10561 aacgcggtgt tgcagcggct gccgcacgtc gctcagcgcg cagcgcgcga tcatgtacgg
    10621 accgtgaaaa agaatctcca cgatatcgag ctggaaggcc accatttgat tcgctcggcg
    10681 gtgccgctgg agatgaacaa ggtgggaatg tgagcagagt tttaccctat ccccaaggcg
    10741 aggggaccgc ccctgcactt aacaggatct ggtgttaaat ctgttcatct ataccctctc
    10801 ccgaatgacc gcccctgcgc taaacaggat ctggtattaa atttgctttt catacaatcg
    10861 gtaacgcttg tacggctccg ccccaatgcg ttccagcatg ttattcatgc ctgtattggt
    10921 ttcgaggatc catgacatct ccagcgcatc gatcttccgg cgggcaaacg gatcgcgtaa
    10981 ggcttcaatc aataacagcg caatcaccgg gccgatgcgg ctgaactgat actcgtcgcg
    11041 cacgcccatc agcggtactc gcgcagttcg cacaccgctg actttcaaac gccacagcaa
    11101 ttttgcccag ccgaagggaa agagcgatcc gttcagatcg gcaatcgcct cgttgatgtt
    11161 aggcaagcca caataaacgc gcagggtgca gaatcaatct cagcgatgta gatcatatcg
    11221 tccggcacca gatatttaag ttggtcgccc atggtcgcga attcatgttc ggtaaacggc
    11281 acaaatcccc agttgtgctg ccagccagag ttgaaaatct cacgcaggat ctgcatctct
    11341 tcggcaaacc gctgacgatt gatgcagcga atggtcacct ttttgcgcac ctgatccatc
    11401 agttttttta gcgccggaga gaaagtgaga tcggttcgct gcatccacca cgccagtaaa
    11461 tcaatgcctt tgtgataacc cagttgttca atatgcgcgg cataccacgg tttgccgtgt
    11521 ggcatcatcg cacagggtgg tgtgtcaaaa ccttcaatca gtaatccgct ttcctgattg
    11581 atattcaggc tgaaaggccc gctgatctta cttgcacctt gtgacttcaa ccacgcttcc
    11641 gctgcgccaa acaacgcggc aaaatcctgc ggatcatcaa tggcgtcaat catgccgaaa
    11701 tgaccggtat cttcaccgta acgctcacgg tgcaaggtat ctatttgcgc ggtaatacgc
    11761 ccaactatct gccccggatt tttcgcagac aaatgctcgt tgcgttcaat gaataaaggg
    11821 ggtatccagt ttggatcgtc gggataaagt gatgacggaa aagcgataaa tgccttaagg
    11881 tcatttttat taattacttt ttcaatttta atcatcagca gatcgcattc catatctctg
    11941 tggataacaa ataattaatg gtgaaaaaca ggttgcaaca gcaacgagcg gcatcttaat
    12001 aatcaactca atgaatacaa acgacgaata aatagtatta attaatgatt atgataaggt
    12061 cagaggctac acagaaacgc ctcaatgcct tttgtcaatt taaggcgacc ggacaaggct
    12121 tcttcttaat atattcataa attaaatatc attcataata aaaacaaaaa acttaaactc
    12181 attatttaaa tcaatgttca ttttcacacc gatgaaaata ctatctcatt tatcttttat
    12241 gcctatataa tccaccgcat tattgtcatt ttatttttga tatccaattt tttcgtggtg
    12301 tatatgtcta ataaaatctt tacgcattcc ctacctatgc gctatgccga ttttccaacg
    12361 ctggttgatg ctttggacta cgccgctctg agtagagccg gaatgaattt ttatgacaga
    12421 cgttgccaac ttgaagatca actggaatat cagacattaa aaacgcgtgc cgaagctggt
    12481 gcgaagcggt tgttatcgct gaacctgaaa aaaggcgaac gcgtggcgct gattgccgaa
    12541 acgagtagcg ggtttgtaga ggcttttttt gcctgtcagt atgctggctt agtcgccgtc
    12601 ccgttggcga ttccaatggg cgtcggtcag cgggattcct ggagcgccaa actccagggt
    12661 ttactggcaa gttgccagcc cgcagccatt atcactggtg atgagtggtt gccactggtc
    12721 aatgccgcga cgcataacaa ccccgaatta catgttttaa gccacgcttg gtttaaggca
    12781 ttaccggaag ccgatgttgc gctccagcgt ccagttccga acgatatcgc ctacctccag
    12841 tacacctccg gcagcacccg ttttccccgt ggcgtcatta tcacccatcg cgaagtgatg
    12901 gctaatctac gtgctataag ccacgatggc attaaattac gccctagcga ccgctgcgtc
    12961 tcctggcagc ctttctacca tgatatggga ctggtcggct ttctcctgcc ccccgtcgcc
    13021 acgcaacttt cagtagatta tttgcgcact caggattttg ccatgcgtcc tttgcaatgg
    13081 cttaaattga tcagtaaaaa tcgcggcacc gtttccgttg cgccgccgtt tggctatgaa
    13141 ttgtgccaac gccgcgtgaa tgaaaaagat ctcgctgaac tggatctttc ctgctggcgc
    13201 gtcgctggta ttggtgctga gccgatctcc gcagaacaac tccatcaatt cgctgaatgt
    13261 ttccgtcagg ttaactttga caataaaact ttcatgccgt gctacggact ggcagaaaat
    13321 gcgctggctg tcagcttctc tgatgaagcc tccggggttg tggttaacga agtggatcgc
    13381 gacatcctcg aatatcaggg taaagccgtc gcgccgggtg cagagacacg cgccgtatcg
    13441 actttcgtca actgcggcaa agcgttgccg gaacatggta ttgaaatccg caatgaagca
    13501 ggtatgccgg tcgcggaacg tgtggtaggc catatttgca tctccggtcc cagtctgatg
    13561 agcggttact ttggcgacca ggtttcgcaa gacgagattg ccgcgacggg ctggttagac
    13621 accggcgacc tcggttatct gctggacggt tatctgtatg tcaccggacg cattaaagat
    13681 ctgattatta ttcgtggccg taatatctgg ccgcaggata ttgaatatat agcggaacag
    13741 gaaccggaaa ttcattctgg cgatgcgatt gcttttgtta ccgcccagga aaaaatcatt
    13801 ttgcagatcc agtgtcggat cagcgacgaa gaacgtcgcg ggcagcttat ccacgcgctg
    13861 gcggcacgga tccaaagcga atttggcgtg accgcggcta tcgatctgtt gccgccccac
    13921 agtattcccc gaacgtcctc cggcaagcct gcccgtgcgg aagcgaaaaa acgttatcag
    13981 aaggcttatg ctgccagtct taatgtgcag gaatccctgg cataaatcaa actgtcgcgg
    14041 tgacgggcgc taccgggttt atcggtaaat atattatcga taacctgctc gcccgcggct
    14101 ttcacgttcg cgcattgacg cgtactgccc gcgctcacgt caatgataat ctcacctggg
    14161 tgcgcggttc gctggaagat actcattcac ttagcgagct ggttgccgga gccagcgtag
    14221 tggtccattg cgccgggcaa gtgcgcgggc acaaagaaga gattttcacc cgctgtaacg
    14281 ttgacggcag cctgcgcctg atgcaagcag caaaagagag cggcttttgc caacgttttc
    14341 tgtttatctc ttcgctggcg gcgcgccatc ccgagctctc ctggtatgca aaatccaaat
    14401 acgtcgccga gcaacgactg gcagcaatgg cggacgaaat tacgctgggc gtttttcgcc
    14461 cgacagccgt gtatggtccc ggcgataaag agttaaaacc gctgtttgac tggatgctac
    14521 gcggcctgct gccacgactt ggtgcgccag atacacaact ctcttttctt cacgtcaccg
    14581 attttgcgca agcagtgggt cagtggttaa gcgccgaaac tgtacagacg caaacctatg
    14641 aattatgcga tggcgtcgcc ggtggctatg actggcaacg cgtacgacag cttgccgcca
    14701 aagcccgttg tggttccgtg cgaatggttg acattcctct gccggtactc acaggccttg
    14761 cggatatcag taccgcgttg agtcgcctgg caggtaaaga acctatgctg acccgctcga
    14821 aaattcgtga attaacccac gccgactggt cggcaagtaa taaccgtatt tctgaagata
    14881 tcaattggtt tcccgggatt agcctggaac acgcattacg caacgggcta ttttgattgt
    14941 cttgttttaa aggtgtgtta acgtttctat ggtaaatcgt gaaatagtaa tggattacat
    15001 cctttcctgt ttacaggatt tggtcgaaaa cggtgtggaa attaagcccg acagcgatct
    15061 cgtcaacgat cttggcctgg agtcgatcaa agtgatggat ctcttaatga tgctggaaga
    15121 cagattcgat atttccattc ctattaatat tctgctggat gtcaaaaccc ctgcgcaatt
    15181 aatggaaact ctacttccat ggctggagaa taaataatgg ggctatacga taaatatgcg
    15241 cgccttgctg gcgagcgtct gcaatttagt gacaacgggt taacaccgtt tggcacctgt
    15301 attgatgaag tgtattccgc caccgaaggc cggatcggca ataaaaaagt gattctggcc
    15361 gggaccaata attatctcgg tttaaccttt aatcatgatg ccatcagcga aggtcaggcc
    15421 gcgttagcgg cccaaggcac cggcaccacc ggttctcgta tggcaaacgg cagttatgcg
    15481 ccgcacctgg cgttagagaa agagattgcc gagtttttca atcgccctac cgctatcgtt
    15541 tttcaaccgg ttataccgct atcgtttttc aaccggttat accgctaatt taggcgtcat
    15601 cagcgcactt gccgaccata acgccgtggt gttactggat gccgacagcc acgccagtat
    15661 ttatgacgtc tgttctctcg gtggcgcgga aattattcgt ttccgccata acgatgctaa
    15721 agatctggaa cggcgcatgg ttcgcctcgg tgaacgggcg aaagaggcga ttatcatcgt
    15781 tgagggcatc tacagcatgc tcggtgacgt tgcgccgctg gcggaaattg ttgatatcaa
    15841 acgtcgtctc ggtggttatt tgattgtcga tgaagcgcat tcgtttggcg tgttaggcgc
    15901 aacggggcgc ggactggctg aagctgtcgg cgttgaagat gatgtggata ttatcgttgg
    15961 tactttcagt aaaagtctgg cctcgattgg cggttttgct gtcgtttcgg aagcgatgga
    16021 agtgctgcgc tacggcagcc gcccgtatat ttttaccgcc tctccttctc cgtcctgcat
    16081 tgcgaccgtg cgctcgtcgt taagaactat tgccacacaa ccagagctgc ggcaaaagct
    16141 gatggataac gccaaccatc tgtatgacgg tctgcaaaaa ctgggttatg agttaagttc
    16201 acatattagc ccggtagtgc cagtaataat cggttcgaaa gaggaaggtt tacgcatctg
    16261 gcgcgagctt atctcgccgg gcgtgtatgt gaatcttatc ctgccgcctg ccgcgcctgc
    16321 aggcatcacg ctgctgcgct gtagcgtaaa tgctgcccac agtcgtgagc aaatcgatgc
    16381 catcattcag gcatttgcta ctctgaagca gtaatgtgag cccgccgtgg tgtttgcgcc
    16441 acggcgggtt taatgttgtc tgtcatacca atacaccagg ctcaacgcca ccaacacaaa
    16501 cgctaccggt aaaccgagcg cggtaacagg tgcgcttaac gcaaatagca atcccagatt
    16561 aaccatgatt tgatagctga tccaggtgag taagcccacg atgacgccta cagcgagacg
    16621 gctacccatc ccaggcgaac gcggggcact aaaggtaaac ggcacagcca gcaaaatcat
    16681 cgccagggtc aggatgggtt gccccagttt ttcccacagt gcaaggtggt attcgctgct
    16741 gggttgcccg gtttcctgca aataatggat gtaatgatta agctgacgga cagaaaaagt
    16801 attccccggc attgataact cttcaagatt catactggtg aaggccgatt gccaggcaag
    16861 attatctaat gtttccagcg tttctttgcc attcagccat tttttatgat tcacaccatg
    16921 caggacccac gttttgtcgt cttcaatggt ggcactgcgt gcataaatgt aggattcaag
    16981 cgagagatcg tcgcgataat gaaatatctc cacgcccacc ggctggccct gctcgttcag
    17041 cgatttcacc gtcacaaatt cattaccacg cctggcccac agcatattgc cggtaatgtc
    17101 atcgtcctcc cccaacgcgg tggcggtcga tttgatttgc aacgcctgtt gctgcaatgg
    17161 cgacgccacc cactcatcta tcgcgcctaa cgaaacagtc cacaatatcc cggcaaccag
    17221 cgccaccagt gcaatacgga agatagaaaa ccccgtgctg cgaatggcgg taagctcact
    17281 gtttttcgat aactgcccca gtccaacaat accacccaat aacgcgataa acggcgaaag
    17341 ttcgaccagt gtgcgcggta aggtcattag caccaccagc accgcctgag tccagcgata
    17401 accgcccggg ctgacaccat ccagttcgtt aatcaggttg aaggtggtaa aaagcggcag
    17461 caatagccct gcagcggcgg caaaaccgag aaagagatga cggattaaat agcgactgaa
    17521 aacattcatc gggaaaattt ctgcagcaaa gagaagtcac gtgcgaccag tatcagcagg
    17581 cccattagca tgagtccggg cactaaccac aaaccaggaa tgaggggaat cgcgccgtta
    17641 gccaccagtg tacggcagac gtcgccgcca taaaaaatgg caataaacag caacgtcagt
    17701 ggcaataacg ttgaaaatcg cccttgccgc ggttttaccc gacttaatga aatggccagc
    17761 agcgccatca acaatgcggt caggccacgg ctttgtcgcc attgcagttc ggcatgatcg
    17821 gcaggaaagg cggagcgcgc cagctccgtg accgatttcg ctttgcgttt gacgttcggg
    17881 ctttgatcca gcggattcag atgtaattgc agattacggt agatctgttc gttgtcgtca
    17941 cgcccctgat gatcgagaag ataggcagtg ccgttgtgca acataacggt cggtttctcg
    18001 ggtgatgggt caaccacatc aaccgaaccg gcgcggaaaa tacgggttcg attggcagta
    18061 gaagtataaa tcagcgcgtc agtcaggcga ttattatcct gatcaaccgt ctgcgaaagg
    18121 atcattcgtc cgttatcgtt agtgttaaat ttctttgccc gcaactggcg aacatccagc
    18181 tccgactgcg actgctgctc cagttgataa atctgcgcat aagcccacgg acgaccatac
    18241 atcgacagaa gggtgacaaa aatacttagc ggtatcgcca gataaagaac ggctttgtac
    18301 aagcgtcccg gactgccccc cgccgcagag atagcggtaa tttccgagtc ggtatacatc
    18361 tgccctagcg tcacgccaac tgacacatac agaccaacag gtaacaacat ctccagtgca
    18421 atcagtactt tgtagaaaac gatatccagc acaacatcaa gagctaatgt cccatttgcc
    18481 gcttcggtca gataacgctg tgcggagtag ctggcgaaaa taaagatcag aaaaccgacg
    18541 ataatcagca ccagacggcg cgtaccacgc atgatgtagt gttcaaccag cttcatctca
    18601 ctaacgccct caatcctttg agttttttat tcattgatca gtttctgcgc caacgcaatg
    18661 acatgatcta cctcttccgg cgtcaggctg ccgtcctttg cccactgaat ttgcccggct
    18721 ttattcagca ccagaatggt tgaactctgc tccggcaagc gccaggcaac gcgtccctgt
    18781 ccattgccgt caataacgaa ttgcgcccac ggatagcggc gtttattttt ttcgattttg
    18841 ccaacaacaa aatagccagt accaaagatg gcatcatcgg tgttcacaat ggtggtgggc
    18901 tgaaaacgat cctgcggaaa gtttgccgct tcaaccgctt taattaacag tgagttcttt
    18961 ttcttcgccg acttacgccc ggcgatatac tgaataatgc ggacttttcc cgctaattgt
    19021 gtgctgttcc acttttggta gtgaatttcg tcgtcgctat tcagaagcag ctcgccgcct
    19081 tcctggatat aaacaggggt aaccgttttg ccagtgacga aattgtgtgc ctgcacaccc
    19141 ggggctaaga gcatgataat caacgcaata atcaaacgag atgacatcat tgctccggct
    19201 atagcggaaa acgcgaaatc agcagctcta agcgctgaca aatatcgcta tccagcgcca
    19261 acggtgcata atacgactgg cggttttcca tatcatccgc caccatgtcg tcgtgtttat
    19321 ctcccatcag gccattaatg gcggcgagga aacgggaaat ctgtaacgcc tctttatttt
    19381 caggatcaag cgatcttaag gtgtcgtaat aagaaacctg ctggctgatg atttttcgca
    19441 catcatgaaa ttccctgccg gttagtcgcg gtttggcaat aatctctttc agggtatgca
    19501 tttgcttttg ccgataggca ataaaactgc ttacgctttc taactggcta tggcgtaatg
    19561 aatagttcac caacgaccag accggagagc ttaaataaat ccgtaacagg ttgccgtaat
    19621 acgacacaat atttttctta ccgttacgaa agccgtcctg aaaacgcccc agaaaaacgg
    19681 tcgttttgcc gaataaaaat ccggcctgat gcttctttaa atacagccgt tgcgcaaaac
    19741 gcaggtgctt gtagcgcgct cgcatgtatt taaattgttt gcgctcatca gcagtcagct
    19801 catcgccttt ggcttgtttg cgaactaaac gcagtaattc ctgacgatca acgccattga
    19861 gccagaattg caggcataac gcgtaattat catgaatgat ttcggtggta caacttagtg
    19921 caatagtcgg cggcaaacta acctgtgcat caacgacatc atcttcttca atcgcaacaa
    19981 atagacgctc gctcgcctca agcgggaaat attttctcat catcaggatt tctctccggc
    20041 tgcgttataa agcaaggaat ggcgctgaaa aaatgccttt aaggttggaa ttaacgagag
    20101 cgccacgtcc agacattgcc caccgaagta agcaaacgct gctcccagca caccaaacag
    20161 atgacagaac atgaacagca atacgatgta gccgattgag gcgatggttt gcgccacgag
    20221 aaaagcgcgc tgttttcccg ccatcaatag caatgactcc tgcgggaagc ccagcatcga
    20281 aatcacaatt gcgcccagca tcacctgaat caggtcatac gcttcgagat acttaacgcc
    20341 aaacaccagc gaaatgagcg gcttgccgac gatcaacacc gcgagcgcca ccagaatacc
    20401 gattccgccc gccagtaaac cggacttcac acctaacaac cacggtctgg tggtgcgcgg
    20461 atctaaacgc atcacctctg ggtaaaaact tttacccagc aaacccgctg gcgttccggc
    20521 ggcgtcgaag aatgtcatgg cgattttaaa taacccggcg gcagcgggtc ctaacacgat
    20581 ccccaccaac actgtgctgc acgagttacg cgccgaccag atggagtggg caatgttggt
    20641 tgaccagaca aaactccacg cgcctttaat gtgtcgggca gactcaaaca gattcaattt
    20701 gaaggcgtta tggatatttc tacggcgtaa ttcgcgcgcg gcaaaccacc agtacatggt
    20761 gccgccaacc agattcgaca cgtaccaggc aataacaaaa cccacaaaac caaagtcaaa
    20821 ataccaggct gcgacgctcc ccgctgcgcg cagaaaaggt ttcgtcgcct gctgtacagc
    20881 aatcaaatca aagcgatcta ccgcacgcag aatgccggtc ggcgtggagg aagccattga
    20941 aggaatgagc gtgcaataca gcgccgccag ccaaaagctt tggtcatcca gacctaatga
    21001 atgggaaagg aatggcagta aggcaatgcc gccgacaatc gccaccgcgc cgctgacgat
    21061 atccagcgag aaggaaaatg agacgacatt gcgaaattgc tgcggattat tgttggtcaa
    21121 ggctggcgtt ccgtactgaa ccaccagctg ccatgtctga aacttaataa aatcgctgat
    21181 cgacttggcg tacgattgca caatcaccag tacaccaaac atggcgggcg tcatcccttt
    21241 accggcacac gagagcgcca acagccccag caaggcgctc tcgacattac tggagcctaa
    21301 ccaggcgcta ttgcgaataa tggtgcgaaa cgcgccatct gcaaaccaat gtttgatgtt
    21361 aaaacccgcc aattcagcct gaccttatcg ttgatagtaa aaaatatccc gccagcctta
    21421 agttaaactt cggcggtcag aaacgatggc aaccagagaa accgccttct gtacctgttc
    21481 cagcacttcg ctgtagggcg ctctggaatc aatctcaaga atttttgtgc cgttatagcc
    21541 aatcgtcgac atgacgccga ttttgtcctg cagctcggca tactcatggt caggcttgcg
    21601 ggagatggca gtatcaatat caatccccaa acgaatgatt aattcaggac ggtattgcgc
    21661 cattttttgg tataaactcc gttcgcgctg cgccagaaac atgctgattt tcccggtcgc
    21721 acgttcgacg ccgatccccg gtccgtcata ataaaagccc gaaatttcag cctgcgggaa
    21781 gcgatcgctg accaccagaa cgccactttg cgccagtcgc tgaaccttgc gtagattcgc
    21841 cattcttcgc agcgagaagc agtacataat caccgccgcc catagcgccg gagatttggt
    21901 tttcatgctt tgggttttcg aggatttggc cgccaatcgc cgttccagcc agacgccaac
    21961 caacggcaat cgtttgattt tgtcgccgtc ttcgccggag agcagcccca gatagcggcg
    22021 ctcggtttgc cagtgttgtt gcagcgattt caccaggtcg gtggtcagcg tggatttacc
    22081 ggtgccatca cacccgacca ccgcaatcag ccccggaatg tagttgggct gcggcgcggt
    22141 tgtactatta acagtttgtg tttgtagtgc atccattcgt taaatcatcc cctatccatt
    22201 ttggtgatca ggaaagcgcg gcgtgaattg cgcgtagtga cgcttgcata atttcatcgg
    22261 ctggttgccg accatccagt tcaaggatct tggcgccatt gaatgtcagt tgcggcgtaa
    22321 cggcgatttt ttcctgtaac gctgccagtt ggtggtcagg tttacgcgca aacgcggttt
    22381 gttcatcaat gccaagacga atcaacaata cgggtaaata agacgccatc cattggtaca
    22441 gcttcaactc gcgctgcctt aacattttta tccaaccgtt accgcccgtg gtttttgcca
    22501 attgcgggcc atcaaagcga aaacccggca cttcaacctg cgggtagcgg tcggtgatga
    22561 gcagatagcc ttgctggctt ttacacaaca ttttgcgaaa cttgtacgcc cgccagcagg
    22621 aaagcagata gataaccagc gcagtaatat tgccaggcgg agttaagggc ttttcgtgta
    22681 catgtgccgc tttacttcgc agataacacc cacaaggtgc gccaataaca gggagctgtg
    22741 aaatccattc gccaattcgc ccggacgatt gcccgagata aatgtgttct gttggcattc
    22801 ttgctgccag ttcatttacc aggcttgccg tgagggtcga tttacctgaa ccatcacatc
    22861 ctacaatggc aataacacgc actggcgtgg aattaattat tgacatatta cgttgattca
    22921 cgaaaaaccc ggcagtaaat aatgtattga atattacgtg gtccgtgctc agaatatccg
    22981 ttcaacctta ttgtgacaat gactaattgt gacattgacc tgaattacgc tttcataaaa
    23041 acatattaac caaataaata tttttaatgg atatttaaat taaaggatat attcatgcag
    23101 tcaataacac ctccattaat tgccgttatt ggtagcgatg cttcaggcaa gtcaacggtg
    23161 tgtgaacatc ttattaccgt tgtcgaaaaa tatggtgctg ccgaaagagt tcatttagga
    23221 aaacaggccg gaaatgtcgg tcgtgcagtg acaaaattac ctttgatggg aaaatcctta
    23281 cataaaacaa ttgaacgaaa tcaggtgaaa acagcaaaaa aattgcctgg accagttccg
    23341 gcgctggtaa ttacagcgtt tgtcgcccgt cgcttactgc gctttcgtca tatgcttgcc
    23401 tgtcgtcgtc gcgggttaat tgttctaacc gaccgttatc ctcaggacca aattcctggc
    23461 gcttacgatg gtacggtgtt cccacctaac gttgaaggtg gtcgttttgt ctcatggctg
    23521 gcaagccagg aacgtaaagc gtttcactgg atggcgagcc ataagcctga tctggtcatc
    23581 aaactcaatg ttgaccttga agttgcctgt gcacgtaaac ccgaccataa acgggaatcg
    23641 ctggcgagga agattgccat aacgccacag ttaacctttg gtggtgcaca actggttgat
    23701 atcgatgcca atcagccact ggaacaggtg ttggttgatg cagaaaaagc gattacggat
    23761 tttatgaccg cgcgtggtta tcactagtca aaaatggaaa tgcccgatcg ccaggaccgg
    23821 gcattttcag gaaggttaaa tcaactgcaa tgctatccag tacagcccac cagaaagaaa
    23881 aattgccgcc ggtaaagtaa acacccacgc catcaggatg ctggttaccg ttttacgctg
    23941 taacccaccg ccgtccacca ccatcgtccc tgctaccgca gacgagagga cgtgtgttgt
    24001 ggagacaggc atcccaatat aactggcaag gccgatagac actgccgccg tcatttgtgc
    24061 cgccatgcct tgcgcatacg tcatgccgcg cttaccaatc ttctcaccga tggtcatcgc
    24121 tacacgacgc cagccaatca tggtgccaat gccgagcgcc agtgctaccg ccatgatgat
    24181 ccacaccgga gcgtactcaa tggtgcttaa catatcgctg cgcagttttt tcagcaggtt
    24241 ctggtcttct ttactgacgc ctggcagttt cgccagcttc gcggaggtat cggagatgca
    24301 cagcataatg cggcgcagct ggctgcgctg actcacgctt aacggctcgt aactttccat
    24361 attgcctggc agcatcgttt taacgcgcgc aatcgcatca aaggtatttg ccggatgaca
    24421 gtgaaactct gttacttgcg tgccatcagt cgatgctgca ggcaatggag gttccatcgc
    24481 aatcaacttc tgcggcagtt caggatgctg ttgcaggtag tgttcgaagt tggtaacggc
    24541 atcgcgggta cgggtaattt catagccgga cgcattcata ttgacgacga agccagcagg
    24601 ggcaatcccc accagtacca gcattaccag gccgatccct ttctgcccgt cgttcgcgcc
    24661 gtgcgaaaac gccacgcccg cagcggaaac aatcagcgca atacgcgtcc agaatggcgg
    24721 tttacgtttg ccctttttct ttttgcgatc ttccggaatg cggtgaatac ggtcacgctt
    24781 tttcgtccca ctccagaaac ggcgcagcag gaaaatcaga ccacccgcaa tgaccaggcc
    24841 gacgataggg gaaacaatca gcgaggagaa aattttggtt acttcacgca ggtttaacgc
    24901 atccatcacc gatgagccgg ttaacagcgc gttggttaaa ccgatgccga taatcgcacc
    24961 aatcaaggtg tgcgaactgg aggccggtaa accaaagaac cacgttccca ggttccagat
    25021 aatcgccgcc agcagcatgg aaaagaccat cgccaaaccg tgagttgacc ccatattcag
    25081 caacaaatcg attggtaaca tatggacaat ggcataggca acgctaagtc cgcccaataa
    25141 cacgccaaaa aagttaaaaa atgccgccat caccacagca agttgtggct gcatggcacg
    25201 agtataaata acggttgcca ccgcattcgc cgtgtcatga aaaccattga ttgcttcgta
    25261 gaacaacaca aatgccagag caagtaataa caaaagcccc gtatatatat caaggccaac
    25321 aaataaattt agcatatatt agattacgcc attttgaatt tacgaacgga cgcattatca
    25381 gtgactttaa cggcatgggc aaagtgaaat atcatttttt aacgataaaa agagtgttat
    25441 ttcttgtgta tcagacaata agcattcatt caaaaccttt agaaataatc aaaacacagg
    25501 tattccactg gttaatatat tcacatatga aatgaataaa tattggaata tataaatatt
    25561 gaatattttg attaatcccc gtactaatta ttcttcataa tcagtacggg gttgctacaa
    25621 catgtattac tttttcacca caattaacgg ttcaatatca ctctcttttt tgatgaccag
    25681 tgattcatca ccgcgcaaac acgtcccacc gtagttgccg ccaacggtga aggtacatac
    25741 ctgaatgtat ttaccgtcca ctttcggcaa acaccacagt tgctgataga tgtttttctg
    25801 ctcggcaaat ttaccgctgg ttttgtccag cacctcttca tgatggctga ccaggtcgat
    25861 attgctacca cagcgaccgg cgatcggttt cactgcgtaa cctgttttca ccaattcatc
    25921 attaacagtg aaatcggtat ccagcaggta acgatggtgc gggaacagcg accagaggat
    25981 cggcagaatc gctttgttgc cggggatcac cgtccacagc ggctcaaaga ccagcacttc
    26041 cgggcgcagc aatacgtcga taagacgcac ttcgttttgc ggatgaccgg tacggattgg
    26101 caccgcagca aactcacggt cgctaacttc acgaatctga tcaaacgcgg tttcccacgc
    26161 ccaggttttc cacacgcagt taaccagtcg cccttcccca tcaatcagtt gcccggcagc
    26221 atcccagccc agttcatcca gcccgcgcaa gatacgcgtt tcaaagcccg cctggtgcag
    26281 cgcctgctcc ataaactgcg cgtgatagtt ttcctcgata tctttgtcct gcatgatatg
    26341 gacaaacgga cgtgcacgac tgtgtttcca ggcaccagcc agttcgttaa tcagcccttc
    26401 cgccggattg aagccgttgc ctttatagcc ctgctccgcc caacgttcga ggatcaagcc
    26461 cgcttcggta tgacaggagg cggagtcggc gttgtattcg taaaccttca ggccacgctc
    26521 atccatgcag aaatccatac gaccagtgat catatggtga cggcgacgct gccaggagag
    26581 acgcaaacgt ggccagagga ttttcggaat gtcgaacagc gccagcaggt tgtcatcttt
    26641 cagcaccttg tcggttgcgt gaagatacat cagatgcagc tcgttggtgg ctttaattag
    26701 ctcctgctcg gcactctcgg taatggtgta gtaatgatac ggatcctgat tgatcacctg
    26761 accgttggcc tgcacatagg cgttttgtag cggatctttt tcatccagcc atttaccgtc
    26821 aaactggcct ttgttttcca gacgcgctcc actgattttc agcagctcgc ctgcaatttc
    26881 cggctgcggt aagctgtatt cagtatcttc cgtctggatc atccagccca gaatggtggt
    26941 gtcatcaaaa gtgtctttaa gggtatagca gccgttttcg accaccatct ccagctcgcg
    27001 cgtccactgt tgcccttgcg gcaacgggga atgaatcacg ttctgttccg caatacggac
    27061 tttgttgcca tgcaattggg taatgatggc gacatggcca gtgtctttaa attcaccgcc
    27121 tttatcccag ataagaagcg cacccgcgac cggcgcacgc ggcgagccgt taggaaatgc
    27181 ctgcaatggc aggatgttgt cattaaccac ttctcgcagg aagcgcagcg agaaaatctc
    27241 ccacgccata cccacgtcag taaagaccac accgtaattc agaaagagaa aacggcgagc
    27301 aaattcaacg cattgccact tgtggcccat atattcgtcg tcgatatagc tacggaatac
    27361 ggcgtcatct tcgtattcct gcggatcgag agaactgtaa tctgaagagt agattgctac
    27421 cccacctggg gcgtagccca ataatgtccc gaacggggca tcctggctgg tcgttccttt
    27481 gctcatcact ttacctttaa caatacaacc taagcagttg gcgtaattgt tgtgctctga
    27541 ttacctgctc agcgacatta accggacaga ggtcagacta atcatacacc tcatcgcagc
    27601 ggctggcgca aagggttaaa aaattcacat tctgtacagc aagtgacctg ctacactgct
    27661 tcaacactac cactcagaag gcaactcact atgacagaca atacttatca gcccgcgaaa
    27721 gtctggacgt gggataaatc cgctggcggc gcgttcgcca atatcaatcg cccgttttct
    27781 ggtccgacgc atgaaaaaac gctgcccgtt ggcaaacatc cattgcaact ttattcgctg
    27841 ggaacgccga acggtcagaa agtaacgatt atgcttgagg agctactggc gctgggcgtt
    27901 actggtgcag agtacgacgc ctggctgatt cgtattggcg atggcgatca attctccagc
    27961 ggctttgtcg aagtgaaccc aaactcgaag atcccggcgc tgcgcgatca tacgcataat
    28021 ccgccgatcc gcgtgtttga atctggttcg atcctgcttt atctggcgga gaaatttggc
    28081 tacttcctgc cgcaggatct ggcaaaacgg actgaaacgt tgaactggct gttctggtta
    28141 cagggtgccg cgccgttcct cggcggtggt tttggtcact tttaccatta cgcgccggtg
    28201 aaaattgagt acgccatcaa ccgctttacc atggaagcca agcgcctgct cgacgtgctg
    28261 gataagcaac tggcgcagca taagtttgtt gcgggcgatg agtacaccat tgcggatatg
    28321 gcgatttggc cgtggtttgg caacgtggtg ctcggcggcg tgtatgatgc cgcagagttt
    28381 ctggatgcgg gaagttataa acatgtgcaa cgctgggcga aagaagtggg cgaacgtccg
    28441 gcagtgaaac gtgggcgtat tgttaaccgc accaacgggc cgctgaacga gcagttgcat
    28501 gagcgccatg acgccagtga ttttgagaca aatacagaag ataagcgtca ggggtaaggg
    28561 ttggtgttcg tcgcagcaag ccatccaggc cggataaggc gttcacgccg catccggcaa
    28621 tcgtgcacaa tgcctgatgc gacgctgccg cgtcttatca ggcctacaaa agctaatcat
    28681 cacgcgctgg taatgtcgta atccatttgg cgcagtgcgt ctaatgtggc tttggcttca
    28741 tcttcgtcga tgatgctcat ggcaaatccg acgtgtacca gcacccactg gcccagcaga
    28801 tcggcagggt taccttcgca aatcagggcg atattcacgt cgcgcttgat accacatact
    28861 tcaacctgcg caagctggtg aatatcttca ccgacagcca gcacctggcc tggaacgcca
    28921 atacacattg ttaactccgg ttatttcgat tgccggacaa acgcgaaaaa gaacctttag
    28981 cgggtgttcc ctctcaccct aaccctctcc ccggaggggc gaggggaccg tccgtgcgct
    29041 ttttaggcgt tgtcctcagt cttattcaac ttcaatactt ttgacgatca gcgaatcgcc
    29101 ggtatcgaca cgcaaccgct cgccgtgaca gagcggacac tgcgcatcgt gctgatgaat
    29161 ctccaccacc tggctgcaat cccagcacca ggcctgggcg ggtttataga cgatatgtaa
    29221 atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac ggacggcgct
    29281 ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc gcttaacatc
    29341 gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagcg acaactcatg
    29401 cattttcgcc cctcctgcgg ccaaacagca gggcgcgacg ccctgcgtgt ggtacatccg
    29461 gattggtgac tggcaacgaa aggatcattc gtgcgcagtc atccgtcagg cgttggcctt
    29521 cttcaatcga catgctgtgc gaaagcggcg acatcagcga acaggagagg tattgcgaaa
    29581 caccgtccag ttcgccaacg gtaaaggtca tggtgccata cggcagttgc agaccaattt
    29641 tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata actgcactca
    29701 gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta aaatcagaaa
    29761 catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc cgggcaattt
    29821 cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct atctcttcag
    29881 tcatgaatcg cctcccgtgg gatggcttcc acgcctgatt cgcgcagcgc agccagaacc
    29941 tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat gtgtggctcc
    30001 agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcacc ggtgaagcgc
    30061 agggccgaca atacgtcggc caggccaagc tgatgcggag agattttgtt ggtaaacaac
    30121 gccggaactt cttcatcccg caggatcatc atcgttcccg gagtgttctt tttcgacaca
    30181 atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag ctccattccc
    30241 gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg ctctaacgct
    30301 tcgacaatcc gcacgccgat ggcttcatcg gtcagcaaaa tattgccgac ccctaagact
    30361 aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca tccactacgt
    30421 gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact tccagcggtt
    30481 tattcggatc ggcaaccggt gtacccacca gcgactgctc gtaaggaccg acgtcatcat
    30541 tgaagttacg cggaccagag ttccaggttg atggaacaac cgcctggtag ttgctgatga
    30601 taccgtcttt aataaccatc cagtgagaga gcataccgcg cggcgcttcg aggaagccaa
    30661 cacctttgaa ctcacccgtt gccggaatgt tcggcttcac aaaggtggtg tgatcgcctt
    30721 tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc aattcacagc
    30781 agtgaacggt acgaccaata atacggccca gcgtggagtg cagctgtgcc acttccagcg
    30841 tgttgccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc tgggtagatt
    30901 cgcgacctgc cgccagtttc accagcatat tagccagcgg acccacttct accgttttgc
    30961 cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa ccatcataag
    31021 ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac caggagtgct
    31081 tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg atcggacgat
    31141 acgaggacag atccgcattc tcaatgtagc ctcccgggaa caggaagctg ccgtttttgc
    31201 tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg cgcgtcagcc
    31261 attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc tcaacaaagt
    31321 cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc aacacgccca
    31381 acccgtcgag gttgattggg ttcgcgacac cacccacagc caggttctga atgtgcggcg
    31441 ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc agcgcctgca
    31501 ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgcc gggtgacccc
    31561 agtagccatt ggcgaaaata cccaactgac cgctggcaac cagatctttg atcttgttct
    31621 gaactttagt gaactcttcc ggactgttca gatgccaggt cgaaacgcct ttcagcattt
    31681 cggaggcttt ggttgggtca gcttgcagtg cagaagtgat gtccacccag tccagcgccg
    31741 aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga atgatgttac
    31801 ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc gcacgaacgg
    31861 aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc atccatgcat
    31921 cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta ccggaagccc
    31981 atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc ccctcaatac
    32041 gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat gacgattatg
    32101 atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga tgtaagcgca
    32161 aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg ggaagtaggc
    32221 gtaaccgccg cccgggttga atgccaccaa cgaataggtc agacgccagg ttgcacaacc
    32281 taacagtgcg ctcagtgctg acaggaacag catacgggaa tcattacgca gcttcgccac
    32341 acgcagaacg accagcggga agagcatcag caggacttca atccagaaca tcacggagta
    32401 gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc caaagcgcag
    32461 cccgatgaaa atcgccagca acacactgat ggtgttggtc agcttgacga acaggctctt
    32521 ttcatccgga ccgttgccac gcagacccgc ctgcaccagt gaaccttcaa agatgacaat
    32581 cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt catagctctg
    32641 ccagagcgga tgcaccttgt agcccgccga gatcatcagc gaccccattg aagactggtg
    32701 catggtcggc agcagcgcac cgagcgcgat gatgaagaac atcaccttgt tcagacgttg
    32761 tagcgacacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca gagccatcac
    32821 gccgatgtag atggtcatac agaccgccgt ctcgaacagt accgagttca cgttgaagtg
    32881 acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag tgatcgacaa
    32941 gccacccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca gcggatggta
    33001 ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac aggcaaagcc
    33061 ggtgccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc cgttcaggtc
    33121 agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac agatgacgat
    33181 taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg gttgtggatc
    33241 atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga cgaaccagca
    33301 cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg gtgtgttgaa
    33361 tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg agattttcat
    33421 aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg ccgtacagat
    33481 gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc gtctgacgcg
    33541 gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc gccatcagct
    33601 cttcacgcgt accgaaaatc accgcgcccg ccgggcacac ttctacgcag ccaggcagac
    33661 cgcctttatc gagacgttcc acacctttct ggttgcacag ctcgcactta tgcagcgcac
    33721 caaacgggtt gttgtagtcg tacttcggca cgttgtacgg acaggcgacc atgcagtaac
    33781 ggcaaccggt gcacacgtct ttgtcgtaat ggacaatgcc ggttttcgga tcttttttca
    33841 gtgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata cactgtttct
    33901 taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg ctggtccaca
    33961 cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag gtctgttccc
    34021 cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa gcctggcagc
    34081 ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc gggcggtttt
    34141 cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg caggaggctg
    34201 ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt tatctttctt
    34261 ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca gcccaaccac
    34321 accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag cgttaacatc
    34381 cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga tgcctttatg
    34441 gaagccgata cctttttcgt tacagccata gcacgggtga ccaatcgcca ccggccacac
    34501 accgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg gccctttaca
    34561 gccgaggtgg tacaagcacc agccttcgcg gtggccttca tcaccgaact ctttggcaaa
    34621 acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac ggccataggc
    34681 gaaggtcgga cggtttttgt catccagttt cggcggtttg ccgtaagtga tgatgtgcgc
    34741 aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga taacggtttt
    34801 gcctggcaga acttcttgca agctgactgc gccagttggg ttaactccag ctgcggcaac
    34861 accgccccac gcggagcagg aaccaatagc gataatggct gctgagcctt ccgccgcttt
    34921 gcggatgtga tccacaatcg gctcaccggc aaccatgcaa taaataccgt tatcttttaa
    34981 tgggatggaa ccatccacca ctaacacata ctgccctttg tacttctcga gagcgttatg
    35041 tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat attccagaga
    35101 gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca gagattccgt
    35161 acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg ggttagtaac
    35221 cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg ctaatgctgc
    35281 acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg tgttatctcc
    35341 agtcatttat agttattccg ttgcgagaac ctggcatata ttttgcctca atcgcaaaat
    35401 caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga tatcatgata
    35461 ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta aagcaagata
    35521 atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg caacgtagaa
    35581 caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc atagcggcgc
    35641 gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc gtacatcctg
    35701 tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt cacattaacc
    35761 gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa atggttattc
    35821 atcgttcacc tcattcatta tatatcgcca tatataacga tattttaggc aaagaacgac
    35881 ttttgcgcac gctatttttt catacttgtt taagacgtaa tcccgtaaga gataaaccta
    35941 tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat cggcggcaga
    36001 tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg gatagatata
    36061 agcctcataa accttattat tgtctttcaa cgccgcctcg taaggaggcc agccctcgtt
    36121 gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct tcgccacatc
    36181 ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg gatacgcgac
    36241 agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca ctttacctgt
    36301 cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca tcagtttggt
    36361 tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat aacctccaac
    36421 ggaacttaat ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc gccgtgccac
    36481 atcttcgata tacggattca gtccacgatt ctcatgcacc acccccacgg ctggcgtttt
    36541 gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac catttggcga
    36601 aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct gagtcgccag
    36661 cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc ccaccgcata
    36721 cttcgccgca aggttgagga actcacgttt cgaaattttc ccgtgaacgt aatagtcgta
    36781 gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg ttgcattcct
    36841 caactggtgt ttttttaagc aaagcataag cgcgtgtttt tgcccagttt ttcgtcactc
    36901 tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca ttgtttcgaa
    36961 attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg ttacgggcag
    37021 atgcagtacc gctattgcgg aaaaagtggt ttacgcctgc ccgcattatc gctcggttta
    37081 tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcaatcct gcgtaaagcg
    37141 tttgatttag gcattacgca ctttgattta gccaacaatt acgggccgcc tccaggaagc
    37201 gcagaagaga actttggtcg cctgctgcgc gaggattttg ctgcttatcg cgatgaactg
    37261 attatctcta ccaaggctgg ctacgatatg tggcccggcc cgtacggctc tggcggttca
    37321 cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct tgagtatgtc
    37381 gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac cgcctctgcg
    37441 ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc ttactcgcca
    37501 gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct tttaattcat
    37561 caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct ggataccctg
    37621 caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt gctgaccgga
    37681 aaatatctca acggcattcc gcaagattca cggatgcatc gtgaagggaa taaagttcgt
    37741 ggtctgacgc cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt attgaatgaa
    37801 atggcacagc agcgtggaca atcaatggcg caaatggcgt taagctggtt gctgaaagat
    37861 gatcgcgtga cgtcggtatt gattggtgcc agccgcgcgg agcaactaga ggagaacgtg
    37921 caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga tcagcatatc
    37981 gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt taataacggg
    38041 cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt caagatagcc
    38101 catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca tcagtttgtt
    38161 atccgggaca tttttcgcat ccataaagac gcgcagtaag tgaatggaag aaattgccac
    38221 aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca gccacttcag
    38281 cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac cggaaaacat
    38341 caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca acacgaggat
    38401 caaatctgat tccgccatcg agaagatatt cggcagtacg tgaataatct cctggaagaa
    38461 cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg caagcagcca
    38521 gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc gtgtagttaa
    38581 aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc aacatatacg
    38641 ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct tatcaacgca
    38701 tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga tgtctcattt
    38761 aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac aaccgacgcc
    38821 aggcatccag gcgaagatga caccgatacc ggattgcggc gagaaaacct atgttggtag
    38881 cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa taggtcgcgc
    38941 tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc ttcccgtgga
    39001 agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca aagccgttct
    39061 gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg aagcgcacaa
    39121 ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg ccattccgga
    39181 tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg ttttcgcgct
    39241 gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagtg tcatcaccac
    39301 ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg ctacgaaggc
    39361 ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag gtattcgggt
    39421 gaatattgtc gcgccaggcc ctatctggac ggcactgcaa atttccggcg gacaaacgca
    39481 ggataagatc ccgcagtttg gtcagaaaac gccgatgaag cgtgcggggc aaccggcgga
    39541 actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca ccgcagaagt
    39601 gcacggcgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt gaaaagcaac
    39661 cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat ccgcctacaa
    39721 acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg tagcgcatca
    39781 ggcattgagc acctaatgcc ggatgcgtcg tgaacgcctt atccggccta caaacgcacg
    39841 aagattcaac atgttgcaat agattttgta ggcctgatga gcgtagcgca tcaggcaatt
    39901 ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag gtaacccgcc
    39961 tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt gtccgctcgg
    40021 aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt aatcattgtt
    40081 tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac cgacagataa
    40141 accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt caccttcaca
    40201 tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa catcacgtcg
    40261 ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt ttcgttaaga
    40321 tgcattgcca tcggacatta tcctgcgcgt aatttttgtg cgacacgaac cggatgcgca
    40381 gcggcgctgg cttccagatc taggtcacgg ctttgcagca acaatacctg cgctgcaaca
    40441 tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata gataacgacc
    40501 gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc gatacccggc
    40561 gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct gttcatgatg
    40621 ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt tgccagatag
    40681 ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag acggaagctg
    40741 gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag ctcattctgc
    40801 gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc gatatcgttg
    40861 gcctggttta aggaacgtgc ttcagccagc agttgctgct cgcgcttaag gcgacgcttc
    40921 tgattgaaga actctacgct cttactgaag aagattgccc aggtgactac agaggccaaa
    40981 ataagcccaa tcatcacgca cttaacgaca atatcggcgt actgatacat accccagacg
    41041 gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc aagtcacaaa
    41101 atctgcgcat aataatatca aaacgacgtc gaattgatag tcgttctcat tactatttgc
    41161 atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag tcaccagcac
    41221 gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg cgaatattca
    41281 tgctagttta gacatccaga cgtataaaaa caggagtccc gacatggcgg acaaaaagct
    41341 tgatactcaa ctggtgaatg caggacgcag caaaaaatac actcttggcg cggtaaatag
    41401 cgtgattcag cgcgcttctt cgctggtatt tgagagtatg gaagccaaaa agcacgcgac
    41461 gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa cccatttttc
    41521 cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgcgctat ttccctgcgg
    41581 ggcggcagcg gttgctaatt cgattcttgc ttttgtcgaa cagggcgatc atgtgttgat
    41641 gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca gcaaactggg
    41701 cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc atctgcagcc
    41761 aaacactaaa atcgtgtttc tggaatcgcc gggttccatc actatggaag tccacgacgt
    41821 tccggcgatt gttgccgccg tgcgcagtgt ggtgccggat gccatcatta tgatcgacaa
    41881 cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg tttctattca
    41941 ggccgccacc aaatatctgg ttgggcattc agatgcgatg attggcactg ccgtgtgcaa
    42001 tgcccgttgc tgggagcagc tacgggaaaa tgcctatctg atgggccaga tggtcgatgc
    42061 cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt tgcgtcaaca
    42121 tcatgaaagc agtctgaaag tggccgaatg gctggcagaa catccgcaag ttgcgcgagt
    42181 taaccaccct gccctgcctg gcagtaaagg acacgaattc tggaaacgag actttacagg
    42241 cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc aatgatgaag agctggcgaa
    42301 ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt atgaatcgtt
    42361 gatcctggca aatcaaccag aacatatcgc cgccattcgc ccacaaggcg agatcgattt
    42421 tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc tgattgccga
    42481 tctggacgcc ggttttgcac ggattgtata acattgccac ttttggacaa ttttgcagac
    42541 attttattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc ggacgattca
    42601 ggagtacaat aggcagataa aggcttaaac gctgttccac aggaaagtcc atggctgtta
    42661 ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg gatcctcata
    42721 ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac ggcctgctgc
    42781 ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg attgcccaag
    42841 gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt ctgggctgct
    42901 ggctaagtta tattcagggg agctggttag ggaataccaa aacggtgaaa ggctggctgg
    42961 cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc cacggtctgc
    43021 tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca accatggcgg
    43081 gaatttccgg tctgccaaac cgccgcttcc agtttttcaa ctggttaagt ggattgctgt
    43141 gggtcagcgt ggtaaccagt tttggctatg ccttaagtat gattccgttc gttaaacgcc
    43201 atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta accgctggct
    43261 tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga cgattttccc
    43321 cgttcccggc tgctgtaccg ggaacgtatt taattcccct gcatcgcccg cattcttgcc
    43381 gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa ttgcgatgcg
    43441 ctttcatagc cgacgcgcat cgctgctgcg ctggccttca tgccgtcatg gatgatcatc
    43501 atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt gctggtgaca
    43561 gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc cagttgctcg
    43621 acgctcaggt tttcggtgta tttattctca attcgtttca gcacgcggct aatcagactg
    43681 aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc ggtcagcacg
    43741 tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag tggtcgctcc
    43801 atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt ggcggagtta
    43861 atcccgctgg ctgccatcga cggctggaaa tgctcatctt cgccaatgtc catcaacagt
    43921 tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac ctctgacgtg
    43981 gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc attggcatca
    44041 taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag aattatgatg
    44101 ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa caaacgcaca
    44161 tcgggcaaca gttctgacaa actattttct ttatttttca gtttattaac tttatccgcc
    44221 agcaagcgac aaatctcttc acgtttcata tcgcgtaatt tcttaggaat aatgcggcaa
    44281 tttgattgtg cgcaattttg tagcatttct ccagcactct ggagaaatag gcaagacatt
    44341 ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac atcaggcaga
    44401 tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa ctttaatctg
    44461 cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg cgaacaaatt
    44521 cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa aaccggcgtt
    44581 ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg cggtattgag
    44641 ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga acagaaagtg
    44701 actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt tatcgccgca
    44761 gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg cggtaaagag
    44821 attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg ttcagaatcc
    44881 aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt ccattctgca
    44941 catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct gccgccgcgt
    45001 caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta tgttaccaaa
    45061 ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac gctaatcgaa
    45121 gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa cgtcatgtgg
    45181 gcggcgactc aggcgctgaa cggtttgatt ggcactggcg taccgcagga ctgggcaacg
    45241 catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca aacactggct
    45301 atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa gctgctgcaa
    45361 tatgctgaac gcgtctggaa catcactgaa ggttccgacg atgagcgtat tgacgccgcg
    45421 attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct ctccgactac
    45481 ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca cggcatgacc
    45541 caactgggcg aaaatcatga cattacgttg gatgtcagcc gccgtatata cgaagccgcc
    45601 cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga cttaagttca
    45661 caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt atggctaatc
    45721 caaccgttat taagctacag gatggcaatg tcatgcccca gctgggactg ggcgtctggc
    45781 aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg ggttatcgct
    45841 cgattgatac cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc ctgaaaaatg
    45901 cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac gaccacaagc
    45961 gcccccgcga agccctgctc gacagcctga aaaaactcca gcttgattat atcgacctct
    46021 acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg aaaggcatga
    46081 tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc cagatccatc
    46141 acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag atcgaacttc
    46201 atccgctgat gcaacaacgc cagctacacg cttggaacgc gacacacaaa atccagaccg
    46261 aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa gtcattcgcg
    46321 atctggcaga taaatacggc aaaaccccgg cgcagattgt tatccgctgg catctggata
    46381 gcggcctggt ggtgatcccg aaatcggtca caccttcacg tattgccgaa aactttgatg
    46441 tctgggattt ccgtctcgac aaagacgaac tcggcaaaat tgcaaaactc gatcagggca
    46501 agcgtctcgg tcccgatcct gaccagttcg gcggctaacg tgcaaattct cccggtggcg
    46561 gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc ccgcccgttg
    46621 ttactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga gccggaagct
    46681 cttaaaagaa caactcatct atatccggga taaacgcaac ggagaggtga aaaacagatg
    46741 aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc gcagcctcct
    46801 tcacagaccg tagaacaaac agtccggcag atttatcaga actataaatc agatgccagt
    46861 acgccttatt tcggtgaaac cggagagcgg gcgataactt cagcgcgtat tcaacaggcg
    46921 cttaccctga acgacaatct tacactaccg ggcaatattg gctggctgga ttatgatccg
    46981 gtttgtgatt gtcaggattt tggcgatctg gtgttagaaa gcgtagcgat aacccaaact
    47041 gacgtcgatc atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga taaagaaaag
    47101 accacacaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga tgatattgtc
    47161 agcaatcatg gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac gctggccgct
    47221 ttagcttcgt tgcagaaaga acagccagaa tcctttgttg ctgaactctt tgaacatatt
    47281 gctgattata gctggccgtg gacgtgggtg gtttccgact cttacctcca ggcggttaat
    47341 gccttctata aaaccacctt caagacggcc aataatcccg atgaagatat gcaaatagaa
    47401 cggcaattta tttacgacaa tccgatctgt tttggcgaag agtcgctatt ttcacgcgtt
    47461 gatgaaattc gagtcctgga gaaaaccgcc gattccgccc gcattcatgt tcgttttacg
    47521 ctgaccaatg gcaacaatga agagcaagag ctggttttac agcggcgcga aggtaagtgg
    47581 gaaatcgctg attttatccg cccgaacagc ggcagcctac ttaagcagat tgaggcaaaa
    47641 accgccgcca gattaaagca atgagctgaa ttaaataaca attagccgga acaataaata
    47701 aaagggaaca ctatatgaaa acgattttca ccgtgggagc tgttgttctg gcaacctgct
    47761 tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcaact ggcgagcaat gtacaaacat
    47821 taaatgccaa aatcgcccgg cttgagcagg atatgaaagc actacgccca caaatctatg
    47881 ctgccaaatc cgaagctaac agagccaata cgcgtcttga tgcacaggac tattttgatt
    47941 gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa tccccggcag catgtcagtt
    48001 gccggggatt ttttttaacg tccaaccgcc gctttagggc gtttcttcgc accagcattc
    48061 accggacgag attgcgtaga cgacgctttt tttgccgtag caggcgtctg acgctgagtc
    48121 gccatcggcg tatgtttagt caacgccgga cgggtattgc ggttctggcg acgagcttca
    48181 cgcatctctt caatggttgg cgcaggcact aagcaatcgc gacggctgcc aatcagatgc
    48241 tttttgccca tcgcttccag cgcctggcgg attaacggcc agtttgccgg atcgtggtaa
    48301 cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct tcggtacgaa gacgccttcg
    48361 ctcttataac caatcttcgc cagcggtttt ttcccggtgt aatacatggt ggttgagttc
    48421 gccagcggcg atgggtagaa gttctgtacc tggtcgagac ggaaacgatg ctttttcagc
    48481 cacagcgcca aattcaccat atcttcatca cgcgtaccgg ggtgcgcaga gatgaaatac
    48541 gggatcagat actgctcttt acctgcctgt ttcgagtagg tatcgaacag ctctttaaag
    48601 cggtcatagc tgcccatgcc cggcttcatc atcttcgaca acggcccttc ttcggtatgt
    48661 tccggagcaa tcttcagata accgccgacg tgatgggtcg ccaactcttt gatatagcgc
    48721 ggatcttcca cggcgatgtc ataacgtacg cctgaggcga taaggatttt tttaatgcct
    48781 ttcagatcac gcgcacggcg atagaggttg atcgtcggtt cgtggttagt gtccatgtgc
    48841 ggacaaatat ccggataaac gcacgacaaa cggcgacaag tttgttcagc gcgtggcgat
    48901 ttgcagcgca acatatacat gttggcagtt ggcccaccga gatcggaaat cacgcccgta
    48961 aaacctggaa cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc ttccgaacgg
    49021 ctctgaataa tgcgcccttc gtgctcggta atagaacaga aagaacagcc gccaaagcag
    49081 ccgcgcataa tgttgaccga aaaacggatc atttcgtaag ccggaatacg ggcattgcca
    49141 taggccggat gtggcacgcg cttgtacggc agcgcaaaaa cgctgtccat ctcttcggta
    49201 gaaagcggga tagcaggcgg gttgatccac acatagcggt caccgtgttt ttgcatcaat
    49261 gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc gcgaagcatg ggcgtacagc
    49321 actttatcgc ccttcacttt ctcgaaagaa ggcagcaata cgtaggtttt ttcccacggt
    49381 ttctggcgcg gtggctgcac gattacagct ttcgcttcct gctttttcgg cgcaacaggt
    49441 ttgttatccg cacacggcaa atcttcgcca tacggatgcg ggattgggtc gattttccct
    49501 agggtatcaa gacgggtgga atccacaccg ctccagccag gcagcgcctc ttttacgata
    49561 attgcggtat tacgcacatc gcggatttca ctaatcggct cgcccatcgc cagacgatgc
    49621 gctacttcca ccagcggacg ttcaccgtta ccaaacatca gcatgtcggc tttcgaatct
    49681 accagaacgg aacggcgcac ggtatcggac cagtaatcat aatgcgcggt acggcgcagg
    49741 ctagcctcaa taccgccgag gatcaccggc acatctttcc acgcctcttt acaacgctgg
    49801 gtataaacca gcgtggcgcg atccggtcgc ttacccgcga cattatctgg cgtgtaggca
    49861 tcatcatgac gtaaacggcg atcggcggta tagcggttga tcatcgaatc catgttgcca
    49921 gcagtaacac caaaaaacag attcggttta cccagacgca taaagtcgtc tttgctgctc
    49981 cagtccggct gggcgatgat cccgacgcga aagccctgcg cttccagcat acgaccgcaa
    50041 atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa aatgatgtcg
    50101 cagctatccc agccaagttg atccatctct tcacgagaca tcggcaaaaa cggtgccggt
    50161 ccaaaacagg cggcccagta ctgcggccag gagaacaggt cgcgatccgg ttggatcagg
    50221 gagatagagc tcattttgct tccagaaatg ataaaaaaat aatcaaaggc cggggattat
    50281 aagccggaac gaaagagaaa tcgaaaggta ttccatactc gccctcctcg ggcgagtatg
    50341 aagattacgg taccggattg accaacagtt gcccaatcga gccacggtcc gccatttcca
    50401 gcgtctgact gttgaagtag aacgggaagt gcgcccagga aggctgaccg aaataaacaa
    50461 gcagctccac ttgtccgtca acccaaacgg tatctttcca gcctctgtct tccgggaacg
    50521 gcatcgcgcc attcacgtta cggatctgga acattacgcc ttcaatatgg aacgcttgcg
    50581 gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg acatcaatac
    50641 ggttgacgtc ccacagctgt ccattaatac ccgggtcatc acccagactg atatcgcgac
    50701 tgcgaattgg cgaaccagcc atgatttcag ttggcagcaa gcgcatcgga agactgtctg
    50761 tgaccagcgg cagaaggccg gttgggcgca gcgttagcac caaggtagaa accagaatac
    50821 tggatggctc aaagaagccg cgaatacgat caacaatgct cgccgcttca ccacaggtga
    50881 tcgacacttc atcgccgttg ctcatatcca ccagaatctc gcggcgctcg cccggtgcca
    50941 gcgaaagttg cttcaccgat acaggagcag gcaggaatcc ctgatcgcca gaaatcacat
    51001 gtaacgggcg accatcgctc atctgtagtt gatagcgacg agagttcgac gcgttcagca
    51061 gtcgcaagcg cacccagcca cgcgagactt caacgtacgg gctttgtaca ccgttaacca
    51121 gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt gtattctggc gtaccaaagt
    51181 tatccagccg tttatcctgg ataatgaccg gaaaatcatc cacaccataa tggttgggga
    51241 taggcagcga cttgctgact tcatcttcca ccagccacat tccggcaagg ccgttataga
    51301 cctgctgagc cgtgcggttg ggagtattgg cgtgatacca cagagtagct gcgttctggc
    51361 gaataggcag tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc ggaccgccca
    51421 tcagcgggcc tggtacctgt agcccggcca ccgtcattga gacattttct gtcaggcggt
    51481 tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg gatagtcggc cccaggtaac
    51541 gaccattgat tccccagacc gacgcgcgtg tccctggcgt aaatgaccag tgcgcacgtt
    51601 gtacagtcat aaacagcggt tgcccacggc gagattcaag tagcggcgga acgggtagcg
    51661 gttgctgttg cccggctgcg ctggccttca ggggaacagc gcctgcacaa agtgcaatcc
    51721 ccgatgcctg aatgaactga cgccgactga gtgacatatt tgctccatgt aaaactggct
    51781 aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac agattgccga cttaaacttt
    51841 tccggcggct tcgcgttctg cgacttcttt atcgagctcg gcgatttttt gttccattat
    51901 cgaacgacaa tgggcagcca gctcacgaac ctgatctttg ccatactgac tgacgtcaat
    51961 tggcggcagc atttcgacaa tcaccagacc gttgtgcagt cgattaagat taatcttatt
    52021 cgaagttgta gagacgcaca cgggaataat cgggacgccc gccgcaattg ccgcgtgaaa
    52081 tgctccagtc ttgaacggta gcaggccgcg accacggctg cgggttcctt ccgggaacat
    52141 ccagatggaa atacggcgtt ttttgaaatg attcacgact tccgcaatgg tgccgtgagc
    52201 tttagtgcga ttgtttctgt cgatcaataa gttgccggtt aaccagtaca actgcccgaa
    52261 gaaggggatc cacagcaagc tctttttacc taccgtcacc gtcggcggtt gcacaatgtt
    52321 cgatgctgtc accatgtcat agttgttctg gtggttagcg atatagatag cattgccgta
    52381 gctttcagca tctgcaggtt tgcggcactc aactttcagg ccgaacagtg gcgcaagacg
    52441 gccaaacata tgcccaaagg tggccacatg tttcgggtta cgcgggctga aaaggcagta
    52501 aatggagccg aatacacaga ctaagatgct gtaaatcacg gtaataataa gacgaaagat
    52561 atatagcata acaccctctg aaggtctgag agactggcat tgtacgtcac ctgaaatcag
    52621 gttagggcat tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc aacgcatgaa
    52681 taaacaacgg tttcaaggga atttttatcg tttccggggg ttgaatacgc tgccggatga
    52741 cgacttaacg tttcatccgg cgttccttgc tagcgggagg aaacagcgcc ctccccggca
    52801 tattactctt cgctatcacc gctgctggca cggcgaggag agtcgatctc aacacgatcg
    52861 atacgctgca aaccgcgcat caacgtaccg cggcgtccac gttcgccagt gactttctgt
    52921 agctcttccg gacgcagttt aattttgcgt ttcccaacat gaatggtcag cgtgctttgc
    52981 ggcggcagaa cgtacagttg cgccagacca tcctcgccac gcgcggcttc tgccgatgga
    53041 atgttgataa tcttgttgcc tttgcccttc gacagctgcg gcagatcgct taccgggaac
    53101 atcaacatac ggcctgcctg agtgattgcc agcagcatat cggaagcatc ttcaatcacc
    53161 accggcggca taacatgggc attttccggt aaggtgatca aagccttacc cgcacggtta
    53221 cgcgctacca gatcgttaaa ggtgcagacg aaaccgtaac ccgcatcgga agccatcagc
    53281 agtttctgat cgtcgctttc catcagcata tggtcaacgg tcgccccagg cggcaacgtt
    53341 aatttgccgg tgagtggttc gccctgacca cgcgccgacg gcagcgtaat cgggtcgatg
    53401 gcatagctac gaccggtgga atcaacaaac actaccggtt ggttactctt acctttcacc
    53461 gccgctttga agctatcacc cgctttataa ttcaggcccg gcgcgtcgat atcatggcct
    53521 ttagcgctgc gtacccagcc catctgcgac agcacaatgg tgacaggttc agacggcagc
    53581 atgtcgtgct cgctcatcgc tttcgcttct tcgcgttcct gcaacggcga acgacgatcg
    53641 tcaccgtagg cttgcgcgtc tgcctgcagt tctttcttca gcaggttatt cattttacgc
    53701 tcggaagcca aaatgccctg caactggtcg cgctcttttt ccagctcact ctgctcaccg
    53761 cgaatcttca tctcttccag tttggcaaga tgacgcagtt tcagttcgag gatcgcttcc
    53821 gcctgggttt ccgtaaggcc aaaccgcgac atcagcgccg gtttcggttc atcttcgtta
    53881 cgaatgatct caatcacttc gtcgatgttg agaaacgcca ccagcaaacc ttccaggata
    53941 tgcaggcgct tgaggacttt ctccagacga tagttcagtc ggcggcgcac ggtatcgcga
    54001 cggaacacca gccattcgga gaggatttcc agcaggtttt tcaccgccgg acgaccatcc
    54061 agaccgatca tattgaggtt aatgcgatag ctcttttcca gatcggtggt agcgaagagg
    54121 tggttcatca cctgatccat atccacgcgg ttggaacgcg gcacaatcac cagacgggtc
    54181 gggttctcgt ggtcagattc atcgcgcaga tcgtcaacca tcggcagctt tttgttgcgc
    54241 atttgcgcag caatttgctc cagtacgcgc gcacctgaaa cctgatgcgg taatgcgctg
    54301 ataaccaccg cgccatcttc tttcttccac accgcgcgca tacgcactga accacgtccg
    54361 ttctcgtaga ttttacgaat ctcggcgcgc gaagtgataa tttccgcttc agtcggataa
    54421 tccggcccct gcacgatatc cagcagctga tcgagcgtag ttttcggctg gtcgattaat
    54481 gcgattgccg cctgagctac ttcacgcagg ttatgcggtg gaatatcggt cgccatgccg
    54541 acggcaatac cggtggtgcc gttaagcaaa atgtttggca gacgggcagg tagcattttc
    54601 ggctcctgca aagtgccgtc gaagtttggc acccagtcag ccgtcccctg ccccagctcg
    54661 ctcaatagca gctcggaata tttcgacaac cgggattcgg tgtaacgcat tgccgcgaac
    54721 gatttcggat cgtccggcgc gccccagttc ccctgaccat caaccagcgg ataacggtaa
    54781 gagaacggct gcgccatcag gaccatcgct tcataacagg cgctatcgcc gtgcggatgg
    54841 tatttaccca gtacgtcacc gacggtacgg gccgattttt taaatttggc gctggcattc
    54901 aggcccagtt cagacatcgc atacacaatg cggcgctgaa caggtttcag accatcacca
    54961 ataaacggca acgcacggtc catgatcacg tacatggagt agtttaagta ggcgttttcc
    55021 gtaaattcat gtagcgcaag gcgctctgcc atatcgctca ttaattctga ttcctcaact
    55081 tattcgccag ccttcaaagg ggcaatattg ccggagatac tacattatct gccgcgtcga
    55141 gtcacaggag atcagcgtga atagcgccgc cggacgtgct ccgacggcga taaaggcatc
    55201 aatgtgcctt gatatacaac tctttgctgt agacttgatc ggcatagagg gcagcacaag
    55261 gttgcgccca tgccacacca ctcggcatgg gggtccgctc cgggcgattc gagaagttga
    55321 ggttatgcga gtccagggaa gcctaaagca tcaaagtagt cgactgtaag tatacggttc
    55381 agcccagttt agctgtgtct ccaccagtgc cccgcgcctc ctgccataag tgcggcttgc
    55441 tcatggatat ccccgagacg gcttacggcg caccggataa taaatttcgc gtatacatgg
    55501 tattccttac cggagaaatt ataagcccaa caacaatacg cctgtgacat tgttcgctca
    55561 aacttatcgc gagtgatttg gctcacactc cggtaaagat tccccatcac gccaacggca
    55621 cataaacatc cgtttccagt cgctgctctg tcaccccttc ggcaagattg gtgtagtgaa
    55681 acagaatcgg tgctttacgc attttctcgc cgcttgcagg cagccagtgg cgaataatgc
    55741 cccatatcgt gtgtgaaata tcgtccagct cgccaacgtg gcgggccacg gcataacgtc
    55801 caccggtaag ttcaccattg ctcacaccat aacgattatc gggaatcggt tcgctaacgc
    55861 tgccgcagat atcaaagcga aacgcttccg gtgcggtggt ggcaggggcg tcccaggcga
    55921 cgccaaaagt ctggctttgg ttaacaggag aaagccccgt ctctttacgc cacatgataa
    55981 atttcgccac actgtagttg agcaactcag ggctacagcg gtgcggcaac atcgccacag
    56041 ggattgatgg gaaatcgata atgtttacat ccagtgtcag gtttgtcatt ttgcctccct
    56101 gcgaaattat tcccgggaac gcgtcccggg agcggtaaca attacggatt tactttgcgg
    56161 atctgtttga cgtcaatttc aacagaattc cagtctttat cgacttcacc ctgaatctca
    56221 accgtatctt tcggcgtcac cgtcacaccg ttccagcgtt tgtgatcgat atcaacattg
    56281 atagtaccgc tggcatcttt aaacacgtag agatcgtcag agatgcgttc aacgatattg
    56341 ccgcgcaggg ttacccaggt gtcgtcacgc agggattttg cgctttctac agtcgttacg
    56401 ctgccgttcg gcccctgaaa tcctccggcc tgactttgcg ttaccgatgg gccagaaaaa
    56461 ccgccctgct ctgctgccat caccggtgca ctgcacaggg ccattactgc gattactgct
    56521 gcgaattttt tcatgtttat tactcccttt aatgtctgtt tccgagcatt taacaagata
    56581 gtccttaaca acttcttaag ggaaaaaaat aaaatttaat gctgtacaga gcgcgttaca
    56641 acacggttta ctggcagcaa atacggttat cgcagggatg aaaaaatgcg aattttactg
    56701 atagaagatg acatgctgat tggtgacggc atcaaaactg gccttagtaa aatgggtttt
    56761 agtgtcgact ggtttacaca aggtcgtcag ggaaaagagg cgctttatag cgcaccttat
    56821 gatgcggtga tcctggattt aaccttacca ggcatggatg gtcgcgatat tttgcgcgaa
    56881 tggcgagaga aaggtcagcg tgagccggta ctgaacctga ccgcgcgtga tgcgttagcg
    56941 gaacgtgtag aagggctgcg tctgggagct gacgattatc tgtgtaaacc ttttgcgttg
    57001 atagaagtcg ccgccaggct ggaggctctg atgcgccgaa ccaacggcca ggccagcaac
    57061 gagctgctcc acggtaacgt catgctcgac cccggcaaac gtatcgccac gctggctggc
    57121 gaacccttaa cactgaaacc aaaagaattt gccctgctgg aattactgat gcgtaacgct
    57181 ggtcgggtac tgccgcgcaa actgattgaa gagaaactgt atacctggga cgaagaggtc
    57241 accagtaatg ccgttgaagt gcatgtgcat catctgcgac gcaaactcgg tagtgatttt
    57301 attcgtaccg tgcatggtat tggctacaca ttaggtgaga aatgaaattt acccaacgtc
    57361 ttagtctgcg cgtcaggctg acgctaatct ttttaattct ggcctcggtg acctggctgc
    57421 tttccagctt tgtcgcctgg aaacaaacaa cggataacgt cgatgaattg ttcgacaccc
    57481 aactgatgct gtttgccaaa cggttaagta cgctcgatct caacgaaatc aacgcggcgg
    57541 atcgcatggc acagacgcca aataaattaa aacacggtca tgttgatgac gatgcgctga
    57601 cctttgccat ctttacccac gacggcagaa tggtccttaa tgatggcgat aacggagaag
    57661 atattcccta tagctatcaa cgggaaggtt ttgctgacgg gcaactggtc ggtgaagacg
    57721 atccttggcg ttttgtctgg atgacctcac ctgatggcaa atatcgcatc gttgttggcc
    57781 aggaatggga ataccgtgaa gacatggcgc tggcgattgt tgccgggcaa ttgatcccgt
    57841 ggctggtcgc actgccgatt atgttaatca tcatgatggt actactgggt cgtgaactcg
    57901 cgccgctgaa caaactggcg ctggcactac gtatgcgtga ccctgactcg gaaaaaccac
    57961 taaacgcgac tggcgtaccc agcgaagtgc ggccactggt tgagtcgcta aatcaactgt
    58021 tcgcccgcac acatgcgatg atggttcgag aacgacgctt tacctccgat gcagctcacg
    58081 aactacgtag cccgttaaca gcgctgaaag tgcaaaccga agttgcgcag ctctctgacg
    58141 atgatccgca ggcgcggaaa aaagcactgc tccaattaca ttccgggatc gatcgcgcta
    58201 ctcgtctggt tgatcaactg ctcacgctat cgcggctgga ctcactggat aaccttcagg
    58261 acgtcgcgga gatcccgctt gaagatctcc tacaatcgtc ggtgatggat atttaccaca
    58321 cggcgcagca ggcgaaaatt gacgtgcgac tgacactcaa tgcccacggc atcaaacgca
    58381 ccggacaacc gctattgcta agtttgttgg tgcgaaattt gctggataac ggccccggtg
    58441 tgacaccaga ggcactggcg cgaattggcg aacgcttcta tcgcccaccc ggacaaaccg
    58501 ctaccggcag tggacttggg ctatcgattg tccagcgaat cgccaagctg catgacatga
    58561 atgttgagtt tgggaatgcg gaacaaggtg gatttgaggc gaaggtaagc tggtaacttc
    58621 tctgtctatg agagccgtta aaacgactct catagatttt actaatagca aaatataaac
    58681 cgtccccaaa aaagccacca accacaaacc agacaaataa cgagcgagaa tacatatagc
    58741 caaaactcga acatttcccg cataaagagt tttcttaaga taagaataat aagtggcgta
    58801 agaagaaaaa atgctgcaat cattgcttgc aatatacgat cttcgcctga aacggaaggt
    58861 atactgcaaa tattgcctcc cgactcaagc atccagtcat acttattgaa agcaaaaaaa
    58921 gaatctatta accaataagt aagcattata tatggcaatg cttcaaatat tgttttaatt
    58981 ttctgcttta acatatatca gtacgctcat aaaaatccat ttataattat caacatgaca
    59041 cgttgatatt ttcatctttt acttattcaa agccatattt tcaatcccca aaaaaaccga
    59101 accaaacgtg taaattattc cggtgcgagt aagaattatg aggaatggct atcagtattg
    59161 tcattttcag aaaatattta tcctgcatcg gtgagtcaga gctagatcag acttttgcta
    59221 aattcgcaaa agactttgca cattttgcta atttcacagt accgctctgt gacgtactat
    59281 agtcgacaaa cgtctcacct tgagattaaa aaatgagcaa catcctgatt atcaacggcg
    59341 cgaaaaaatt cgcccactcc aatggtcaac tgaacgacac cctgaccgaa gtcgcggatg
    59401 gcacactgcg cgacctcggg catgatgtcc gcatcgttcg cgccgacagc gactacaatg
    59461 tcaaagcgga agtacaaaac tttctctggg ctgatgtggt gatctggcag atgccaggct
    59521 ggtggatggg cgcgccgtgg acagtgaaaa aatatattga tgatgtattc accgaaggtc
    59581 acgggactct gtatgccagc gatggtcgta cccgcaaaga tccgtcgaaa aaatacggtt
    59641 ccggcagcct ggtacaggac aaaaaatata tgctttctct aacctggaac gcaccaatgg
    59701 aagccttcac cgaaaaagat cagttcttcc acggcgttgg cgttgacggt gtgtatctgc
    59761 cgttccataa agcaaaccaa ttcctcggta tggaaccgct gccgacattt atcgctaatg
    59821 acgtgataaa aatgcctgat gttccccgct atactgaaga atatcgcaag catcttgtgg
    59881 aaatttttgg ttaactagag ctcaggcttt agaaggagtt aaccatgctt accgtaatcg
    59941 cagaaatccg tactcgtcct ggtcaacatc accgtcaggc ggtattggat cagtttgcta
    60001 aaatcgttcc aaccgtactg aaagaagaag gttgccacgg ctatgcgcca atggtggatt
    60061 gcgctgctgg cgtgagtttc cagtctatgg caccggattc tatcgtgatg attgagcagt
    60121 gggaaagcat cgcgcacctt gaagcgcatc tgcaaacccc gcacatgaag gcgtacagcg
    60181 aagccgtaaa aggcgacgtg ctggagatga atatccgtat tctgcagcca gggatttaat
    60241 cctgccttgt ttgcccggcc atcctgaccg ggcaatgttc tttcctttaa acctcaatct
    60301 ccgccatgtc gcctttctct tgcaaccagt tgcggcgatc ttccgagcgt ttcttcgcca
    60361 gcagcatatc catcatcgcg tcagtacgct gatcgtcttc atcatcgata gtcaactgca
    60421 ccagacggcg agtgttcgga tcaagcgtgg tttcacgcaa ttgcatcggg ttcatttccc
    60481 ccagaccttt aaaacgctgg acgttcggct tgcctttctt gcgttttaat tgctcaagta
    60541 cgccctcttt ctcttcttcc gtcagcgcgt aataaacctc tttcccgaga tcaatacggt
    60601 agagcggtgg cagtgcgacg taaacgtgac cgtgtttcac caacgcgcgg aagtgtttta
    60661 cgaacaaagc gcagagcagc gtggcaatgt gcagaccatc agagtccgca tccgcgagga
    60721 tacagatttt gccataacga agctggctca gatcgtcgct gtcaggatcg ataccgatcg
    60781 ctaccgaaat atcatgcact tcctgcgagg ccagcacttc gtcggaagaa acttcccagg
    60841 tgttaaggat cttacctttc agcggcatga tcgcctgata ttcacgatcg cgcgcctgct
    60901 tggcagatcc gcctgcggag tcaccttcca cgaggaacag ttcggtacgg ttaaggccct
    60961 gcgcggtaca atcagccagt ttgccaggca gcgccgggcc gctggtcagc tttttacgca
    61021 ccactttttt agccgcacgc atacgacgct gggcgctgga aatcgccatc tctgccagca
    61081 actccgccgc ctgaacgttc tggttcagcc acaggatgaa ggcatctttc accacgcccg
    61141 aaacgaatgc cgcacactga cgcgaagaga gacgctcttt ggtctgcccg gcaaactgcg
    61201 gatcctgcat tttaaccgac agaacatagg cgcagcgatc ccagatatct tccgccgaca
    61261 gcttcacgcc gcgcggcaga atgttgcggt attcacagaa ctcacgcatc gcgtccaaca
    61321 ggccctgacg cagaccatta acatgggtac cgccctgcat cgttgggata aggttgacgt
    61381 agctttcggt cagcagttca ccgccttccg gcagccacag tagcgcccag tccacagctt
    61441 cagtatcgcc agcgaaatta ccgataaacg gtttttctgg cagcgtcggt aaaccgttta
    61501 ccgcttccgc caggtaatca ttcagaccgt cctgatagca ccagcgctgt tcggtgttgt
    61561 tgatctcatc tttaaaggta atctcaacgc cggggcacag tactgctttg gccttcagca
    61621 catgcgtcag gcgtgaaaca gaaaaacgcg ggctgtcaaa gaaggtttca tccggccaga
    61681 agtgcacgct ggtaccggta ttgcgtttac cgcaagtgcc gacaacctgt aaatcctgca
    61741 ccttttcgcc attttcaaag gcgatgttat aaacctgacc atcgcggcgc acgttaactt
    61801 ctacgcgctt cgacaaggcg ttaactaccg aaatccccac gccatgcagg ccgccagaga
    61861 actggtaatt tttgttagag aatttaccgc ctgcatgcag acggcaaaga atcagttcaa
    61921 ccgccggtac tccttcttcc gggtgaatat ccaccggcat cccgcgccca tcgtcaatca
    61981 cttctaacga ctggtcagca tgtaagatta catccacgcg ttttgcgtga cccgccagtg
    62041 cttcatcgac actgttatca ataacttctt gccccaaatg gttagggcga gtggtatcgg
    62101 tatacatccc cggacggcgg cgaaccggct caagcccggt gagtacctca atggcatcag
    62161 cgttataagt ttgcgtcatg gtttaagtta gcaattcgag ttgatcgtca gagatggtgc
    62221 agaccaagaa aatcgacgat cgggttgaaa taatcttcga agcccgtgaa tgcgtggttg
    62281 ccgccttcta tgacagtctg gcggcaggaa gcatagtacg ccaccgcctg gcggtaatcc
    62341 agcacttcat ctcccgtctg ttgcagcagc cagatcaaat ccggcgcttc cagcgggtca
    62401 atctgcatga ctttgagatc gtaaatatgg cgtgactcta gcacatattg ctgcccggtg
    62461 taggggttct cgttctgacc gagatagtcc gtcagcaatt caaacgggcg taccgccggg
    62521 tttaccacca ctgcgggcag cataaaacat tgcgacaacc aggtggcgta atatcccccc
    62581 agtgacgaac cgacaatacc cagcgaatcg ccgccatgtt ccaggacaat ggattccagc
    62641 agttctgccg cgtcggaagg atacggcggc aactgcggaa tgatcatctc aacgtcaggg
    62701 tgatgttccg ccagccagtt ttttaacaag ctcgcttttg cagagcgcgg cgagctgttg
    62761 aaaccgtgta aataaagaag cgtagacatc agtagccttc tgaagcggta tcaggttgga
    62821 aacgtgtgtc cgccaggcga tgcacctcgg tggtcagcgt gccatcagca tgtaactcga
    62881 gagtacgcca gccgggcgcg atagtatcca gcgtaaagtt ggaacagtgc ggcttaaact
    62941 gcacacaggt cgacggcgtt gccagcaggc ggcgaccgtt ccagtcgaga tccagctcct
    63001 gatgaatatg accgcacagc aagtatttga cgtgcggaaa cttcgccagc acggtatcca
    63061 gttcgcccgc gttacgcaga ctgtgttgat cgagccaact acaacccgca ggtagcggat
    63121 gatgatgcag cagcagcaac gtatggcgtt ctggcgcatc ggccagttta cgttccagcc
    63181 actcaagctg aaactcgctc agctcaccgt gcggcacgcc aaacacctgg ctatccagca
    63241 acaggatttg ccattgctca ccaataaaca cccgcttcgc cggggagata cccgcatcct
    63301 gtaacgcgct gtacatcgcg ggctggaaat cgtggttgcc cggcagccag acgcagggcg
    63361 cacgaaaact tgcgatgcct tcagcgaaat gctgataggc cgcagaggat tgatcctgcg
    63421 ctaaatcacc tgtcgcgaca atcaggtcga attcgtgctg gtgtggacga atcgcctcca
    63481 gcaccgcctg gtagctttcc caggtgttta cccctaacag ggcttcgtgc ttttgtgcaa
    63541 acaggtgagt atcggtaatt tgtaaaatcc ttcctctggc ctcaccagcc agaggaaggg
    63601 ttaacaggct ttccaaatgg tgtccttagg tttcacgacg ctagtaaacc ggaatcgcca
    63661 tcgctccatg tgctaaacag tatcgcaacc agtccgctaa aaactggtta atttgatgct
    63721 tttcgtcgcg ttgatgcaac tttttattag gataatcata ccgcgctttg aagcgaaaaa
    63781 tctgctggct tgaacacact tcagccacca tcgcgtcatg atacagacgc accgtcattg
    63841 acggaaggct ccagtaactg atcgcgggcg cagtctgttc tattgtcacc agggtagtgt
    63901 atcgggtcga ttccacaatc gtcagccgat attgtgcgtt tgccacctga tagcttacag
    63961 tttcgccggg tgcgtcattg cgcggtaaca aacggcgcaa ttgtgaaaag ttcatctcgc
    64021 acaggcgcat catttcagga aagtcaggtg tgtaacgctt catttatgcc cactcatttt
    64081 ttaacgcttg atgatgcagc tgcagccatt gcaaagcgat gaccgacgct gcgttgtcga
    64141 ttttcccctc ttctacccac tggtatgcct gttcccggct taccacatga acgcgaatat
    64201 cttcgttttc atcagccaga ccatgaatac cgcttgcggt cgtggcgtcc acttcgccca
    64261 ccataattga cgaacgctca ctggtgcccc ccgggcttgc caggaaactt aacaccggtt
    64321 tggtccgttt gactatcagt cccgcctctt caatcgcttc gcgacgggca acatcttcca
    64381 cactttcacc ctcttcaatc atcccggcaa ccatctccag tagccagggg gtttcgctgg
    64441 tgtcgtacgc ggcaatccga atctgctcaa tcagcacaac ttcatcacgc actgggtcaa
    64501 agggtagcaa gactgcggcg tgaccgcgct caaaaatttc ccgccgtacc tcatgactca
    64561 tttgcccgtt gaatagacga tgacgaaatc tataaagatc taatgaaaaa aagccgcgat
    64621 aaagtgtttc tcgtgcaata atttctacat cgtttttgcc aaatgtaacg ggcaggttgt
    64681 ctggcttaag cattgttaat gtcctagcac taatagtgaa ttaaatgtga atttcagcga
    64741 cgtttgactg ccgtttgagc agtcatatgt taaattgagg cacattaacg ccctatggca
    64801 cgtaacgcca accttttgcg gtagcggctt ctgctagaat ccgcaataat tttacagttt
    64861 gatcgcgcta aatactgctt caccacaagg aatgcaaatg aagaaattgc tccccattct
    64921 tatcggcctg agcctttctg ggttcagttc gttgagccag gccgagaacc tgatgcaagt
    64981 ttatcagcaa gcacgcctta gtaacccgga attgcgtaag tctgccgccg atcgtgatgc
    65041 tgcctttgaa aaaattaatg aagcgcgcag tccattactg ccacagctag gtttaggtgc
    65101 agattacacc tatagcaacg gctaccgcga cgcgaacggc atcaactcga acgcgaccag
    65161 tgcgtccctg cagttaactc aatccatttt tgatatgtcg aaatggcgtg cgttaacgct
    65221 gcaggaaaaa gcagcaggga ttcaggacgt cacatatcag accgatcagc aaaccttgat
    65281 cctcaacacc gcgaccgctt atttcaacgt gttgaatgct attgacgttc tttcctatac
    65341 acaggcacaa aaagaagcga tctaccgtca attagatcaa accacccaac gttttaacgt
    65401 gggcctggta gcgatcaccg acgtgcagaa cgcccgcgcg cagtacgata ccgtgctggc
    65461 gaacgaagtg accgcacgta ataaccttga taacgcggta gagcagctgc gccagatcac
    65521 cggtaactac tatccggaac tggcggcgct gaatgtcgaa aactttaaaa ccgacaaacc
    65581 acagccggtt aacgcgctgc tgaaagaagc cgaaaaacgc aacctgtcgc tgttacaggc
    65641 acgcttgagc caggacctgg agcgcgagca aattcgccag gcgcaggatg gtcacttacc
    65701 gactctggat ttaacggctt ctaccgggat ttctgacacc tcttatagcg gttcgaaaac
    65761 ccgtggtgcc gctggtaccc agtatgacga tagcaatatg ggccagaaca aagttggcct
    65821 gagcttctcg ctgccgattt atcagggcgg aatggttaac tcgcaggtga aacaggcaca
    65881 gtacaacttt gttggtgcca gtgagcaact ggaaagcgca catcgtagcg tcgtgcaaac
    65941 cgtacgttcc tccttcaaca acattaatgc ttctatcagt agtattaacg cctacaaaca
    66001 agccgtagtt tccgcgcaaa gctcattaga cgcgatggaa gcgggctact cggtcggtac
    66061 gcgtaccatt gttgatgtgt tggatgcgac caccacgctg tacaacgcca agcaagagct
    66121 ggcgaatgcg cgttataact acctgattaa tcagctgaat attaagtcag ccctgggtac
    66181 gttgaacgag caggatttgc tggcactgaa caatgcgctg agcaaaccgg tttccactaa
    66241 tccggaaaac gttgccccgc aaacgccgga acagaatgct attgctgatg gttatgcgcc
    66301 tgatagcccg gcacccgtcg ttcagcaaac atccgcacgc actaccacca gtaacggtca
    66361 taaccctttc cgtaactgat gacgacgacg gggcttcggc cccgtctgaa cgtaaggcaa
    66421 cgtaaagata cgggttatct gccgcattct tcccccttct cgcttcaatt tcgaccagcc
    66481 atcctctatt ctgatgggta tttaccactg gtcccggaag acaaaaatga aacggacaaa
    66541 atccatacgc cacgcatcgt tccgcaaaaa ctggagcgca cgccatctga caccagtcgc
    66601 tctcgcggtt gccactgttt ttatgctggc tggctgtgaa aagagtgatg aaacagtgtc
    66661 tctctatcaa aatgctgacg actgttcagc tgcaaaccca ggcaaaagcg ccgaatgtac
    66721 caccgcgtac aacaatgcgc tgaaagaagc cgaacgtact gcgccgaaat acgccacccg
    66781 tgaagactgt gttgctgaat ttggtgaagg tcagtgtcag caagcaccag cccaggctgg
    66841 catggcacca gaaaaccagg cgcaggctca gcaatccagc gggagtttct ggatgccgct
    66901 gatggccggt tacatgatgg ggcgtctgat gggcggcggc gcgggatttg cacagcagcc
    66961 gctgttctcc tcgaaaaacc cggccagtcc ggcttacggt aaatataccg acgcgacggg
    67021 taaaaactat ggcgcagccc agccaggccg caccatgacc gtaccgaaga cggcaatggc
    67081 accaaaaccg gcgaccacca ctaccgttac ccgtggcggt tttggtgaat ctgttgccaa
    67141 acaaagcact atgcagcgta gcgcaaccgg cacctcttct cgttcaatgg gtggctgata
    67201 ccgatggaaa gagtcagtat taccgagcgc ccggactggc gtgagaaagc ccacgaatac
    67261 ggtttcaatt ttcacaccat gtacggcgag ccgtactggt gtgaagatgc ttactacaag
    67321 ttgaccctcg cccaggttga aaagctggaa gaagtcaccg ccgaactgca ccagatgtgc
    67381 ctgaaagtgg tggaaaaagt gatcgccagc gatgagctga tgaccaaatt ccgcatacca
    67441 aaacacacct ggagttttgt gcgtcaatca tggctgactc accagccatc gctttattcg
    67501 cgtcttgatc tggcgtggga tggcactggc gaacctaaac ttctggaaaa taacgccgat
    67561 acgccaacgt cactatacga ggcggcgttc tttcagtgga tctggctgga agatcagctt
    67621 aacgccggta acttgccgga gggcagcgac cagtttaaca gtctgcaaga aaaactgatc
    67681 gatcgcttcg ttgagctgcg tgaacagtat ggcttccagt tgctgcatct cacctgctgt
    67741 cgcgacacgg tggaagatcg cggaaccatt cagtatttgc aggactgcgc aaccgaagct
    67801 gaaatcgcca ccgagttcct ctacatcgac gagataggtt taggcgaaaa aggccagttc
    67861 acggatttac aggatcaggt gatttctaac ctgttcaagc tttatccgtg ggaatttatg
    67921 ctgcgcgaaa tgttctccac caagctggaa gatgctggcg tacgctggct ggaaccggcg
    67981 tggaagagca ttatctccaa caaggcactt ctaccgctac tgtgggagat gttcccgaat
    68041 cacccgaacc tgctgcccgc ttattttgcg gaagatgatc atccgcaaat ggaaaaatat
    68101 gtggttaaac cgatcttctc ccgtgaaggc gcaaacgtgt cgatcattga gaacggcaaa
    68161 accattgaag cagcggaagg tccgtatggc gaagaaggga tgattgttca gcaattccac
    68221 ccgttaccga aattcggcga cagctatatg ctgattggta gctggctggt gaacgatcaa
    68281 cctgccggaa ttggcattcg tgaagaccgt gcgttgatca cccaggatat gtctcggttt
    68341 tatccacata tttttgttga ataagccacg ataccggatg gcactcgcca tccggtaatt
    68401 gttagcctat ctgcaccgac agcatactta aactgcccat ttctatacca tcgaccggaa
    68461 tggtaattgg ctcctgccca tcccacacac ctaacacata caacaacggc agatagtgct
    68521 ctggcgttgg gttcgataac gcgccacctt catggtcgag ataattcacc agaggatgct
    68581 gctctaccgg cccttgccac gtcagattcg ctttcacata ctcattaaac gacgtcgccc
    68641 acggatacgg tgaactatca ccgtgccact tcactgtgcg caggttatgc accacgttac
    68701 cgctggcgac caacattatt ccttcatcac gcagcgccgc gagtttgcgc cccatttcga
    68761 aatgccaggc ggcaggtttg ctactgtcga tactcaactg caccataggg atatcggcgt
    68821 caggatacat cttaatcagc acgccccacg agccgtggtc aaagccccag gcttctttat
    68881 ccagcgtcac cgggatcggc gctaacagct caaccagacg ctgtgccagc gcaggcgaac
    68941 ccggagcagg ataatgcgta tcgtacagcg cctgcgggaa gccaccaaag tcatgaatcg
    69001 tgggcggcgt ctccatcgcg gtcactcctg ttccacgggt aaaccagtga gccgaaacca
    69061 ccacaatcgc ttgcgggcgt ggcaatgtca tccccaactt ctgccagctg cgggtataca
    69121 aattatcttc cagcacgttc atcggactac cgtgacctaa aaacaatgct ggcatacgtg
    69181 ttgaagacat gatgatatcc ttaactaaag gtgtcatttt gatatcctca caatacgctt
    69241 gttcggcgga gtaagaaccc ggataacaat gatgatgatc atcagttatt ttgacgatct
    69301 gcctgaaggt gaagatttat aaggagttgt cgatgtcagt acctctcatt ctgaccatac
    69361 tggcgggggc agccacgttt attggcgcgt ttctcggcgt tctcggacaa aaaccctcga
    69421 accgcttact ggcgttttcg ctaggttttg cggcggggat catgttgctc atctcattaa
    69481 tggaaatgct tcctgccgca ctagcagctg aaggaatgtc gccggtgttg ggttatggaa
    69541 tgtttatctt cggtctgctt ggctactttg gcctggaccg catgttgccg catgcgcatc
    69601 cgcaggattt aatgcaaaaa tcggtgcagc tgttgccaaa atcgatcaag cgcacagcca
    69661 ttctgctcac tctcggcatc agtctgcata acttcccgga agggattgcc acctttgtca
    69721 cggcgagcag caacctggag ctgggatttg gcatcgcact ggccgtcgcg ttgcacaata
    69781 tccctgaagg tctggcagtg gcaggcccgg tttatgcggc aacggggtct aaacgcaccg
    69841 cgattctgtg ggcggggatt tctggactag cagaaattct tggtggtgtg ctggcgtggt
    69901 taatcctcgg tagcatgatt tccccggtgg tcatggcggc aatcatggcg gcggttgcag
    69961 gaattatggt ggcgctctcg gttgatgaat taatgccgct cgccaaagag attgacccta
    70021 ataataaccc cagctatggc gtactatgtg gaatgtcagt gatgggattc agtttagtgc
    70081 tgctacaaac ggcgggaatt ggttaaaaac aacatcagaa gttgtccagc acatggacaa
    70141 cttctgccag cttcacgaaa gccttattct accttttcgg ctgtctcttc tctcgtactg
    70201 tttaagtatt tgttccgcgc ttcaagcagt gcgtctctgt cctggcgaaa cgcccgacaa
    70261 taacgtttca aatgcaggaa ataacccacc acaaccagag caatcaatac aatccagtac
    70321 caggcgataa acatcctttt acccttaata tttaacaata ctaaacattt agcgcataaa
    70381 tttcacatat cctttttcgg atatattcat atggtcgcat aataacggaa caattatatg
    70441 attttgatca taatgaaaaa gaatgcctga tattaatgaa attttctcat agaaagagaa
    70501 gcggaattaa catcttgcat agcagggcaa aataaaaacc gaagccagct acaacttcgg
    70561 tttcttgatt aaggcagtaa ttaagcagcg gttttcagct ggctttacgc tcatgtgcct
    70621 gacggtatgc caccaggtct tcaatagtca cgagcgccat attgtgttta ttggcaaact
    70681 caatgcactc tggtgcacgc gccatcgtgc catcgtcatt agtcagctca cacagcacac
    70741 cagccggttt aaagcctgcc agcgtcatca aatcaatagt cgcttcagta tgaccgccac
    70801 gcgtcagtac accacctgcc tgcgcgcgaa gtgggaaaac gtggcccggg cgattcagat
    70861 cagaaggttt tgcgccatcg gcaatcgctg cgcgaacggt cgtaatacga tcagcggcag
    70921 aaacaccggt agtcacacct tcagccgctt caatggtcac ggtaaaaccg gtgccatagg
    70981 cgctggtgtt attttctacc atcattggca gatcaagttg tttacggcga tcttcagtga
    71041 tgcacaggca aacaataccg ctaccgtggc gaatggtcag cgccatctgc tcaacggtca
    71101 tggtttctgc cgggaagatc atatcgcctt cgttttcacg gtcttcatca tcaagcacca
    71161 ttacaccgcg tccttcacgc agcgcagcca gtgcattttc aacacgttcg aaaggcgtac
    71221 caaaagagga aagtagcgtc tgattcatgg taaaaaaacc tcactaaaat tatggttacc
    71281 agaatcaggg cagtcttagg agtggcggca tatagccaaa ataacgtgag cgggtccatg
    71341 cccgacagaa tcgttactct ctcccatccg gactctaacc gtcggccccg gaattacacc
    71401 ggatctgctg tcctttgagt tcgcacccaa agcgctcgcg ggctttcaac tgagttgatt
    71461 taccgccggt ggggaatttc gccccgccct gagaataagc ggattcacta taacgctaat
    71521 gattagcggc agcaacgcat agcttcacat aattctggtt tatgacttac ccttatcgca
    71581 ctacaatggt actcaacacc tatcagtaca gggaaactac aatgattgac ccgaaaaaaa
    71641 ttgagcaaat cgctcgccag gttcacgaat caatgcctaa aggaatcagg gagttcgggg
    71701 aagatgtgga gaaaaaaatc cgccaaaccc tacaagctca gctgacgcgc ctcgaccttg
    71761 taagccgtga agagttcgac gtccaaacgc aagtcctgtt acggactcgt gagaaactgg
    71821 cgctgcttga acaaagaatc agcgaactgg aaaatcgcag tactgagatc aaaaagcagc
    71881 cagatccaga gactctcccc ccaacgctgt aaacatttgt ctttaatgca ctgtaaatta
    71941 tgaattaatc ctctaattta cagtgttttc tattacatta tcgcttaatc ccattcagag
    72001 tatcatcaca aaaatgaatg attgcaattg aaaatataat tgttatgtat tctttaagtt
    72061 ttacttaatc gata
//
LOCUS       DAJTSF010000006        66985 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000006 DAJTSF010000000
VERSION     DAJTSF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.006"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(66..1070)
                     /gene="cra"
                     /locus_tag="OO335_000456"
     CDS             complement(66..1070)
                     /gene="cra"
                     /locus_tag="OO335_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6000967.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1152..1238)
                     /locus_tag="OO335_000457"
     CDS             complement(1152..1238)
                     /locus_tag="OO335_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6000968.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1250..1741)
                     /gene="ilvN"
                     /locus_tag="OO335_000458"
     CDS             complement(1250..1741)
                     /gene="ilvN"
                     /locus_tag="OO335_000458"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6000969.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1744..3468)
                     /gene="ilvI"
                     /locus_tag="OO335_000459"
     CDS             complement(1744..3468)
                     /gene="ilvI"
                     /locus_tag="OO335_000459"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6000970.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3587..3631
                     /locus_tag="OO335_000460"
     CDS             3587..3631
                     /locus_tag="OO335_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6000971.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3786..4730)
                     /gene="leuO"
                     /locus_tag="OO335_000461"
     CDS             complement(3786..4730)
                     /gene="leuO"
                     /locus_tag="OO335_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6000972.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5389..5475
                     /gene="leuL"
                     /locus_tag="OO335_000462"
     CDS             5389..5475
                     /gene="leuL"
                     /locus_tag="OO335_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6000973.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5568..7139
                     /gene="leuA"
                     /locus_tag="OO335_000463"
     CDS             5568..7139
                     /gene="leuA"
                     /locus_tag="OO335_000463"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6000974.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7139..8230
                     /gene="leuB"
                     /locus_tag="OO335_000464"
     CDS             7139..8230
                     /gene="leuB"
                     /locus_tag="OO335_000464"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6000975.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8233..9633
                     /gene="leuC"
                     /locus_tag="OO335_000465"
     CDS             8233..9633
                     /gene="leuC"
                     /locus_tag="OO335_000465"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6000976.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9644..10249
                     /gene="leuD"
                     /locus_tag="OO335_000466"
     CDS             9644..10249
                     /gene="leuD"
                     /locus_tag="OO335_000466"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6000977.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10298..11476)
                     /gene="setA"
                     /locus_tag="OO335_000467"
     CDS             complement(10298..11476)
                     /gene="setA"
                     /locus_tag="OO335_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR6000978.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11578..11709)
                     /gene="sgrT"
                     /locus_tag="OO335_000468"
     CDS             complement(11578..11709)
                     /gene="sgrT"
                     /locus_tag="OO335_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6000979.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11798..13453
                     /gene="sgrR"
                     /locus_tag="OO335_000469"
     CDS             11798..13453
                     /gene="sgrR"
                     /locus_tag="OO335_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6000980.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13487..13586
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13617..14600
                     /gene="thiB"
                     /locus_tag="OO335_000470"
     CDS             13617..14600
                     /gene="thiB"
                     /locus_tag="OO335_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6000981.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14576..16186
                     /gene="thiP"
                     /locus_tag="OO335_000471"
     CDS             14576..16186
                     /gene="thiP"
                     /locus_tag="OO335_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6000982.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16170..16868
                     /gene="thiQ"
                     /locus_tag="OO335_000472"
     CDS             16170..16868
                     /gene="thiQ"
                     /locus_tag="OO335_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6000983.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16982..17746)
                     /locus_tag="OO335_000473"
     CDS             complement(16982..17746)
                     /locus_tag="OO335_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6000984.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17832..18710)
                     /gene="araC"
                     /locus_tag="OO335_000474"
     CDS             complement(17832..18710)
                     /gene="araC"
                     /locus_tag="OO335_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6000985.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19049..20749
                     /gene="araB"
                     /locus_tag="OO335_000475"
     CDS             19049..20749
                     /gene="araB"
                     /locus_tag="OO335_000475"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR6000986.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20760..22262
                     /gene="araA"
                     /locus_tag="OO335_000476"
     CDS             20760..22262
                     /gene="araA"
                     /locus_tag="OO335_000476"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6000987.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22462..23157
                     /gene="araD"
                     /locus_tag="OO335_000477"
     CDS             22462..23157
                     /gene="araD"
                     /locus_tag="OO335_000477"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6000988.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGTKAYYGQ"
     gene            23232..25583
                     /gene="polB"
                     /locus_tag="OO335_000478"
     CDS             23232..25583
                     /gene="polB"
                     /locus_tag="OO335_000478"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6000989.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLIPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25748..28654
                     /gene="rapA"
                     /locus_tag="OO335_000479"
     CDS             25748..28654
                     /gene="rapA"
                     /locus_tag="OO335_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6000990.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28666..29325
                     /gene="rluA"
                     /locus_tag="OO335_000480"
     CDS             28666..29325
                     /gene="rluA"
                     /locus_tag="OO335_000480"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6000991.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29442..30257)
                     /gene="djlA"
                     /locus_tag="OO335_000481"
     CDS             complement(29442..30257)
                     /gene="djlA"
                     /locus_tag="OO335_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6000992.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30512..32866
                     /gene="lptD"
                     /locus_tag="OO335_000482"
     CDS             30512..32866
                     /gene="lptD"
                     /locus_tag="OO335_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6000993.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32919..34205
                     /gene="surA"
                     /locus_tag="OO335_000483"
     CDS             32919..34205
                     /gene="surA"
                     /locus_tag="OO335_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6000994.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34205..35194
                     /gene="pdxA"
                     /locus_tag="OO335_000484"
     CDS             34205..35194
                     /gene="pdxA"
                     /locus_tag="OO335_000484"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6000995.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35191..36012
                     /gene="rsmA"
                     /locus_tag="OO335_000485"
     CDS             35191..36012
                     /gene="rsmA"
                     /locus_tag="OO335_000485"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6000996.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36015..36392
                     /gene="apaG"
                     /locus_tag="OO335_000486"
     CDS             36015..36392
                     /gene="apaG"
                     /locus_tag="OO335_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6000997.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36399..37241
                     /gene="apaH"
                     /locus_tag="OO335_000487"
     CDS             36399..37241
                     /gene="apaH"
                     /locus_tag="OO335_000487"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6000998.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37319..37798)
                     /gene="folA"
                     /locus_tag="OO335_000488"
     CDS             complement(37319..37798)
                     /gene="folA"
                     /locus_tag="OO335_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6000999.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37989..39851)
                     /gene="kefC"
                     /locus_tag="OO335_000489"
     CDS             complement(37989..39851)
                     /gene="kefC"
                     /locus_tag="OO335_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6001000.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39844..40374)
                     /gene="kefF"
                     /locus_tag="OO335_000490"
     CDS             complement(39844..40374)
                     /gene="kefF"
                     /locus_tag="OO335_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6001001.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40422..41816)
                     /locus_tag="OO335_000491"
     CDS             complement(40422..41816)
                     /locus_tag="OO335_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6001002.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41875..42162)
                     /gene="fixX"
                     /locus_tag="OO335_000492"
     CDS             complement(41875..42162)
                     /gene="fixX"
                     /locus_tag="OO335_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR6001003.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42159..43445)
                     /locus_tag="OO335_000493"
     CDS             complement(42159..43445)
                     /locus_tag="OO335_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6001004.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43496..44437)
                     /locus_tag="OO335_000494"
                     /pseudo
     CDS             complement(43496..44437)
                     /locus_tag="OO335_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44452..45222)
                     /locus_tag="OO335_000495"
     CDS             complement(44452..45222)
                     /locus_tag="OO335_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR6001005.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45578..47092
                     /gene="caiT"
                     /locus_tag="OO335_000496"
     CDS             45578..47092
                     /gene="caiT"
                     /locus_tag="OO335_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR6001006.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFDSCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47123..48265
                     /gene="caiA"
                     /locus_tag="OO335_000497"
     CDS             47123..48265
                     /gene="caiA"
                     /locus_tag="OO335_000497"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR6001007.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48394..49611
                     /gene="caiB"
                     /locus_tag="OO335_000498"
     CDS             48394..49611
                     /gene="caiB"
                     /locus_tag="OO335_000498"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR6001008.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49685..51238
                     /gene="caiC"
                     /locus_tag="OO335_000499"
     CDS             49685..51238
                     /gene="caiC"
                     /locus_tag="OO335_000499"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR6001009.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51347..52132
                     /gene="caiD"
                     /locus_tag="OO335_000500"
     CDS             51347..52132
                     /gene="caiD"
                     /locus_tag="OO335_000500"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR6001010.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52138..52728
                     /gene="caiE"
                     /locus_tag="OO335_000501"
     CDS             52138..52728
                     /gene="caiE"
                     /locus_tag="OO335_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR6001011.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52814..53209)
                     /gene="caiF"
                     /locus_tag="OO335_000502"
     CDS             complement(52814..53209)
                     /gene="caiF"
                     /locus_tag="OO335_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR6001012.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53471..56692)
                     /gene="carB"
                     /locus_tag="OO335_000503"
     CDS             complement(53471..56692)
                     /gene="carB"
                     /locus_tag="OO335_000503"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6001013.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56710..57858)
                     /gene="carA"
                     /locus_tag="OO335_000504"
     CDS             complement(56710..57858)
                     /gene="carA"
                     /locus_tag="OO335_000504"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6001014.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58314..59135)
                     /gene="dapB"
                     /locus_tag="OO335_000505"
     CDS             complement(58314..59135)
                     /gene="dapB"
                     /locus_tag="OO335_000505"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6001015.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59302..60216)
                     /gene="rihC"
                     /locus_tag="OO335_000506"
     CDS             complement(59302..60216)
                     /gene="rihC"
                     /locus_tag="OO335_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6001016.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60282..61232)
                     /gene="ispH"
                     /locus_tag="OO335_000507"
                     /gene_synonym="lytB"
     CDS             complement(60282..61232)
                     /gene="ispH"
                     /locus_tag="OO335_000507"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6001017.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61234..61683)
                     /gene="fkpB"
                     /locus_tag="OO335_000508"
     CDS             complement(61234..61683)
                     /gene="fkpB"
                     /locus_tag="OO335_000508"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6001018.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61770..62264)
                     /gene="lspA"
                     /locus_tag="OO335_000509"
     CDS             complement(61770..62264)
                     /gene="lspA"
                     /locus_tag="OO335_000509"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6001019.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62264..65080)
                     /gene="ileS"
                     /locus_tag="OO335_000510"
     CDS             complement(62264..65080)
                     /gene="ileS"
                     /locus_tag="OO335_000510"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6001020.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65123..66064)
                     /gene="ribF"
                     /locus_tag="OO335_000511"
     CDS             complement(65123..66064)
                     /gene="ribF"
                     /locus_tag="OO335_000511"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6001021.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66072..66290)
                     /locus_tag="OO335_000512"
     CDS             complement(66072..66290)
                     /locus_tag="OO335_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6001022.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66393..66656
                     /gene="rpsT"
                     /locus_tag="OO335_000513"
     CDS             66393..66656
                     /gene="rpsT"
                     /locus_tag="OO335_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6001023.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16250 a        18238 c        17243 g        15254 t
ORIGIN      
        1 gatgtccagt cccgtactct acgcgccaga gggaaattca cctggcgcgt atttttgttc
       61 gcggattagc tacggctgag cacgccgcgg cgatagagat tacgtttaat gcgcgttaaa
      121 ccaggttttg gcttacgcgg ttcgtccagg cttgccagga caatctccag cacacgctct
      181 gcgacatcgc ggtgacgttg agccactgcc agcaccggac actgtaagaa gtcgagcagt
      241 tcgttatcgc caaaggtggc aattgccagg tcagaaggca gtttgccgtc gcgacgcagc
      301 gtgacatcca tcactccttg caacaacgca aacgacgttg tgaacagcgc ctgcggcatc
      361 ggatgcgttt ccagccattt ttcgaataac tgggcagccg cctcccgctc atagctgttg
      421 gcatacagga aatgcacttc gcgcggatca tctttccaag cagtacggaa accttgttca
      481 cgcaggaagc tgacagaaag ctccggcagc gcgccaagat aaagcaccgt ctcggcggga
      541 aacttacgta actcttccgc cagcatttcg gcatcatcct gatcggcacc aaccacgctg
      601 gtgaaatgtt cacgatcgag ggcgcggtcc agcgcgacaa tcgggaacgg gtcgttagcc
      661 cagcgttgat aaaaaggatg ctcaggtggc aacgacgtcg aaacaataat ggcatcaacc
      721 tgacgctgta aaaggtgctc aatgcagcgc atttcgttgt ctggctgatc ttctgagcag
      781 gcaatcagca gttgataacc ccgttgccgc gcctggcgtt caagatagtt agcgatgcgg
      841 gtatagctgg tgttctccag atcggggatc acaagaccaa tagaacgtgt gcgtccagca
      901 cgaagcccag ctgccacggc gttcgggtgg taattgtgct cacgcaccac agccatgact
      961 ttttcaacgg ttttgtcgct cacacggtat tgcttcgctt tgccgttaat aacatagctt
     1021 gcagtggtcc gcgacactcc cgccagccga gcgatttcat ccagtttcac aattgcccct
     1081 tgcgtaaaat gtaaaaacca tagccattgt actggtatgg gttaaattca ttaacatcta
     1141 agcgcagaaa attaactgcg gcaactgctt ttattccagg ttcccactga tttcgcaaaa
     1201 aaagcccaac gtgacatgtt gggctgcaaa ttgcgcactg agatcattct caacgcatta
     1261 ttttatcgcc gcgcgaaagt ccgaccacac cagagcgagc aacttccaca attttcgcca
     1321 catcgcgaat cgatgctaaa aatgcatcaa gcttatcgct ggtgcctgct aattgaacgg
     1381 tataaagcga gggggtgaca tcgataattt gcccacggaa tatttccgta ttacgtttca
     1441 cttcgtcacg cccgtaaccg ctggcctgaa ttttcaccag catgatttcc cgctcaacat
     1501 gcgcgccctg ccccaactca ctcacgcgca agacatcgac cagcttgtgt aattgctttt
     1561 cgatctgctc aagtactttt tcatcgccca cggtctggat ggtcatacgc gataatgtcg
     1621 gatcgtcggt tggcgcaacg gtcaggcttt caatgttgta gcctcgctgg gaaaaaaggc
     1681 caatcacgcg ggataacgcg cctgattcat tttcgagtaa gactgataat atccggcgca
     1741 taatcaggtt ctctccgttt tgcttaacca catttcatcc attccgcccc cgcgaatctg
     1801 catcgggtag acgtgctcac tgccatcgac ggtaacatca acaaacacca agcgattatt
     1861 gcgcacctgt tccagcgcct cgctaagttt gctttccagc tcatgcggat gagagatctg
     1921 gatcccgacg tgaccatagg cttccgccag acggacgaaa tcgggtagtg attgcatata
     1981 agattgtgaa tgacggccgg aatagatcat gtcctgccac tgtttcacca tccccagata
     2041 gcggttattg agattcacca ccagtacggg caactcgtat tgcaacgcgg tagacagttc
     2101 ctggatgttc atctgaatac tgccgtcgcc agtgacgcaa accacggttt cttctggcaa
     2161 cgccattttg acgcccagtg ccgcaggtaa accaaaaccc atcgtgccga gaccgccgga
     2221 attgatccag cgacgcggtt tgtcgaatgg ataataaagc gcagcaaaca tctggtgctg
     2281 cccgacatcg gacgtcacgt aagcgtcccc attagtcaac cgccaaagag tctcgatcac
     2341 cgcctgcggt ttaatctttt cactgtgagt gtcatatttc aggcactgaa gagcgcgcca
     2401 ctgttcaatc tgctgccacc agtcgcggat ctcatccagc ggttgatggg cggattcttg
     2461 cgacaggagt tcaagcattt gttcgaggac ctggcgagca tcccccacaa tcgggatatc
     2521 cgcagtcacg gttttagaaa tggaagtagg atcaatatcg atatgcagaa cagtggcatt
     2581 tgggcagtac tttgccagat tgttcgtcgt tcggtcatca aatcgtaccc cgacggcgaa
     2641 aatcacatcc gcgttatgca tcgtcatatt ggcttcgtag gtaccgtgca ttcccagcat
     2701 gcccagtgcc tgacgatgcg ttgccggaaa cgcccccatt cccatcaatg aggaaacaac
     2761 gggcagattc aacgcctcca ccgtttcttt caactgctga tggcagcccg ccatgattgc
     2821 cccaccgcct acgtagacaa ccggtttttt tgccgctacc agcgtttgca gagcacgctt
     2881 aatttgccct ttatgtccgg tagtagtggg attgtaagaa cgcatactga ccgactcagg
     2941 gcagacatag ggtaatttgt tcgccggatt aagaatatct ttcggtaaat caacgaccac
     3001 tggtcctggg cgaccacttg ccgccagcca gaaagccttt ttcagcacct gcggaatgtc
     3061 ttccgtttgc ttaaccagaa aactgtgttt aaccaccggt cgcgaaatcc ccaccatgtc
     3121 gcactcctga aaggcatcgt aacctatcaa cgaggtcgct acctgcccgg aaaggacaac
     3181 taatggaatg gaatccatat aagcggtggc gatgccggta atcgcattgg tcgcccttgg
     3241 acccgacgtt accagcacga cgccgacttc cccggtcgca cgcgccaggc catcggccat
     3301 atgcaccgcc gcctgctcat gacgaactaa tacatgatca ataccaccca cggtatgcaa
     3361 tgcatcgtaa atatcaagga ctgcgcctcc gggataaccg aatacttgtt taacgccctg
     3421 atcgataagc gatcggacga ccatctcggc tccagacaac atctccatgg cctgcctcac
     3481 tgtttgacgg aaaaaatgtg taaaacacat taaccgtccg atcttgctta agcaattggc
     3541 agccagagac ataaacgcag ggtaataaga ataaacagga ggaaaggtga aaagaaacga
     3601 taaaatcctc cattacgaag gattaaattg ataataaatc ggctgaatcc cacaacttac
     3661 agcaatttct ctattttttt aattaatcct aaaatagaga gtccacacat tcagaataaa
     3721 attctacatt caataaaaaa tgcagaataa accagacatt catgtctggc ctattctgca
     3781 ttcagttagc gtttgcaaat tgagactaat tgttcttcca tccactgatg gcctttatcg
     3841 cgcccggcag cttcatgcca agagagataa caggttctgc tgttttgttt taacggcagc
     3901 ggtaatacct gtaattctaa ggattcagcg aactcttcag ccagccaacg cggcgcaata
     3961 gcgaccaaat gcgtttgcga caccacgcta agtacgctca tcattgccat gccctgatac
     4021 gcgatactgg cttgcttatc taccgtgtca taccaaggtt gactaaatga cgcgaaacga
     4081 tcgagcgaaa ccgccgcatg ttgttcgtta taaacatcat gtttcagtaa cgggccttta
     4141 attgttggat gatttttgct ggctaccagc accatttcat ctttaaataa tggcacgctg
     4201 gtaaattcag gacgatggaa gtcttcataa ctaatcacaa actccgtttc ctgataacgc
     4261 agctgatgtt cagtgttctg atttaatgaa gacttgaaca taacatgtat atttggcgca
     4321 atctgctcaa tgtgattata aatctgcgag gtcagaatgc tgtctaacgg gctgcaaaca
     4381 caaagatgaa atacacgttc actgctcgcg ggttcaaaac ctgaaccagg caattcattt
     4441 tgtactagtt gcaatgcctg gcgaactgaa ccaaaaagtt gaaatgcgcg agcagtcggt
     4501 tgaataccac ggccataacg aacaaaaagc tcgtcattaa acatcacctt caggcgtgca
     4561 acagcgttac tgaccgcagg ttgcgacata cccagaacat gagcggcacg ggtaatgttt
     4621 tgctcctgca tcacggcatc gaaaacggtt aataagttga gatcgaccat gcgtagctgt
     4681 ggcttgctta actccgccgt ctctgaatga tctgtttgta cctctggcat acttaactcc
     4741 actgtcacac ttaactccct ttcccttatt ggaatgcaga gaaataatcc tgaagaatat
     4801 gatttatcat gcatataaaa taagaaaaag caaaatgagt aaaattcgga aaacataaag
     4861 atacgggttc tgataaaaac agaatccatt gccgtggaat gagtcattta ccacagcata
     4921 ataatccata atgttttgtt gcgaaaacaa tctaatcata actaccgcga atactcaatc
     4981 atctacaaaa tggattaaat gtgcttattt aataattaat ttatatattt aatgcattaa
     5041 ttcttaacat taattgttca ataatattca ccaaatcaat atcaaaaaaa atcgcaaaac
     5101 atataattca atacaaatca tcaggatagg ttttgcaacg cgtgcatttt gtcccctttt
     5161 tcctcgttga ttagatgcaa aaatttatgc tgaaatatgt caaccgatga aaagcgtcgg
     5221 tagttaagca gaaattaata tcgcttactt taaccaccgt agcacaatta gctaatttta
     5281 cggatgcaga actcacgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt
     5341 gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc
     5401 gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag acgagtgagc
     5461 ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt
     5521 ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag
     5581 tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg
     5641 tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat gggtgttgac gtgatggaag
     5701 tcggtttccc cgtctcttcg ccgggcgatt ttgaatcggt gcaaaccatc gcccgccagg
     5761 ttaaaaacag ccgcgtatgt gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg
     5821 ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgct acctccccaa
     5881 tgcacatcgc caccaagctg cgcagcaccc tagacgaggt gatcgaacgc gctatctata
     5941 tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc
     6001 gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca
     6061 ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca
     6121 gcggcctgta tgaacgcgta cctaacatcg acaaagccat tatttccgta catacccacg
     6181 acgatttagg cctggcggtc ggcaactcac tggcggcggt acatgccggt gcacgccagg
     6241 tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg gaagaagtca
     6301 tcatggcgat taaagttcgt aaggatattc tcaacgtcca caccgccatt aatcaccaag
     6361 agatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca
     6421 aagccattgt tggcagcggc gcattcgctc actcctccgg tatccaccag gatggcgtgc
     6481 tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc
     6541 agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg
     6601 ggtataaaga aagtgaatat aatttagaca atttgtacga tgctttcctg aagctggcgg
     6661 acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc
     6721 aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata
     6781 tcgccaccgc cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg
     6841 gtaacggtcc ggtcgatgcc gtctatcagg caattaaccg catcactgac tataacgtcg
     6901 aactggtgaa atacagcctg accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg
     6961 atattgtcac caactacaac ggtcgccgct tccacggcgt cggcctggcc accgatattg
     7021 tcgagtcctc cgccaaagcc atggtgcacg tactgaacaa tatctggcgt gccgcagaag
     7081 tcgaaaaaga gttgcaacgc aaaactcaac acaacgaaaa caacaaggaa accgtgtgat
     7141 gtcgaagaat taccatattg ccgtattgcc gggagacggt attggtccgg aagtgatgac
     7201 ccaggcgctg aaagtgctga atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag
     7261 ccattacgat gtaggcggcg cagccattga taaccacggg caaccactgc cgcctgcgac
     7321 ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg
     7381 ggaacattta ccaccagacc agcaaccaga acgcggcgcg ctgctgccgc tgcgtaagca
     7441 cttcaaatta ttcagcaacc tgcgcccggc aaaactgtat caggggctgg aagcattctg
     7501 tccgctgcgt gcagacattg ccgctaacgg cttcgacatc cagtgcgtgc gcgaactgac
     7561 cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc
     7621 gtttgatacc gaggtgtatc accgttttga gatcgagcgt attgcccgca tcgcgtttga
     7681 atctgctcgt aaacgtcgcc acaaagtcac ctcaatcgac aaagctaacg tgctgcaatc
     7741 ctctatttta tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg atgtcgaact
     7801 ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga
     7861 cgttctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac
     7921 tggctcgatg ggcatgttgc cttccgccag cctgaacgag caaggttttg gactgtatga
     7981 accggcgggc ggctcggcac cagatatcgc aggcaaaaac atcgccaacc cgattgcaca
     8041 aatcctgtcg ctggcactgc tgctgcgtta cagcctggat gccgatgatg cggcttccgc
     8101 cattgaacgc gccattaacc gtgcattaga agaaggcatt cgcaccgggg atttagcccg
     8161 tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga
     8221 aggggtgtaa tcatggctaa aacgttatac gaaaaattat tcgacgctca cgtagtgtac
     8281 gaagccgaaa acgaaacccc gctgttatat atcgaccgcc acctggtgca tgaagtgacc
     8341 tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa
     8401 accttcgcca ctatggatca caacgtctct acccagacca aagacattaa tgcctgtggt
     8461 gaaatggcgc gcatccagat gcaggagctg attaaaaact gcaaagaatt tggcgtcgag
     8521 ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg taatggggcc ggaacagggc
     8581 gtcaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac ccacggcgcg
     8641 tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacacaa
     8701 accctgaaac agggccgtgc gaagaccatg aaaattgaag tccagggcaa agccgcgccg
     8761 ggcattaccg caaaagatat cgtgctggca attatcggta aaactggcag cgcaggcggc
     8821 accgggcatg tggtggagtt ttgcggcgaa gcaattcgtg atttaagcat ggaaggtcgt
     8881 atgaccctgt gcaatatggc gatcgaaatg ggcgcaaaag ccgctctggt tgctcctgac
     8941 gaaaccacct ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac
     9001 gacgccgttg cctactggaa aaccctgcaa accgacgaag gcgcaacttt cgataccgtt
     9061 gtcactctgc aagcagaaga aatttcaccg caggtcacct ggggcaccaa tcccggccag
     9121 gtgatttctg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgt
     9181 gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac cgggtattcc gctgaccgaa
     9241 gtggctatcg acaaagtgtt tatcggttcc tgtaccaact ctcgcattga agatttacgc
     9301 gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt
     9361 cccggctctg gcccggtaaa agcccaggcg gaagcggaag gtctggataa aatatttatt
     9421 gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac
     9481 cgtctgaatc cgggcgaacg ttgtgcatcc accagcaacc gtaactttga aggccgccag
     9541 gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc
     9601 ggacatttcg ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat
     9661 caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat
     9721 cccgaaacag tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga
     9781 ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttccc
     9841 gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg
     9901 tgagcacgcg ccctgggcat tgaccgacta cggttttaaa gtggtgattg cgccgagttt
     9961 tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga
    10021 tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat ccggggatcc atttcgacgt
    10081 ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc
    10141 cttccgccgc cactgcatga tgaatggtct ggacagcatc ggcctgaccc tgcagcacga
    10201 cgacgctatt gcgtcttatg aagcaaaaca acctgcgttt atgaattaga ccccttgccc
    10261 ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca aacgtcttta acctttgcgg
    10321 ttaaaaataa tgcgacaaca gaaataatcg caattaccca gtagacagca aagtgccccc
    10381 aactttgtgc aattgctccc tgaataacac cagccagaat taccccggta gagatagtgt
    10441 tggtaaataa ggtggtagct gcccccgctc ttccaggcat taaatcctga aaccatagca
    10501 tcccaatacc cgcaacaatg ccgataaata cagcgttaaa aagttgcagc gtcatcaacg
    10561 ccatacggct atgaaagaaa atcaatccag tgtaaaacag cactcctgcc accactgcaa
    10621 tgaccatcat tcgccgctta ccataacgtt tgacatagta gccagccaga atcattgctg
    10681 gtatttccag tccagctgcc gtccccatca ggaaacccgc cagtttgtct ggcaatccta
    10741 aatcgctact gatccacaat ggcatatcaa taatgtacat agtgttgcag gtccacatta
    10801 acgtcgaggc gacaaataac atccgtacgt tactatcctg ccagtcacct tgcattgata
    10861 aagcattttc cgacggcagt tctacccgcg ccacagacag aagcataaat gcaatcaata
    10921 ccagactgag tgtgaatatc ccggcggcaa tcgaaaacat cactgtaaag ccgtaattca
    10981 acgccagcat aaaggccagc ggtggaccga ttacccatgc cagagaaagc tgcgcacgca
    11041 tcaccgagct aaacatcacc acttctcgcg ccgagttatc cgcgtattcc cgcgccagag
    11101 caaataactg tggcattgcc gtattggcca gagatgccag aagcacacca caggtgataa
    11161 gcgtcagata atgacgatta aatgcaaaca gtagcgcatt gccaatagcc atcaaacagc
    11221 aaaatataat cagttttcgc cgatcgccct gactgtcaga acgttttgcc aaccagaggc
    11281 ttacgccgat cccagcaata gcattcaccg tataaaagag tccgatccag aaaggttgcg
    11341 cgccaacctc acgactcaga aataagctca atgtaggagc ctgtagcgcc ccggccaccc
    11401 ccatcataaa agcgaccagc ataaatgccg cgtaaacacc gttcatacgg cgagccatcg
    11461 tcattatcca gatcatacgt tcccttttta gcgcggcgag aataaaaaaa accagcaggt
    11521 ataatctgct ggcgggtgat tttacaccaa tactcagtca cacatgatgc aggcaagtca
    11581 actttcagaa ttgcggtcat cccactgcat cagtccttca agcatggtta atcgttgtgg
    11641 gacgcttaac caacgcaacc agcacaactt cgctgtcgcg gtaaaatagt gctgataaaa
    11701 ctgacgcatg gggcacctcc ttgcttcatc gttaggaatt attgacttaa tatagggaaa
    11761 ataaaattgc tgtcttttgc gcaggagttc cccttttatg ccatctgctc gtctgcaaca
    11821 acagttcatc cgcctgtggc aatgctgcga gggtaaatcg caggacacaa cgctcaacga
    11881 actggcagcg ttattgagct gctcgcgtcg tcatatgcgt accctgctca acaccatgca
    11941 ggatcgcggc tggctgacgt gggaagcgga agtcgggcgc ggtaaacgct cgcgtctgac
    12001 attcctctat accgggctgg cgcttcagca acagcgggcg gaagacctgc tggagcagga
    12061 tcgtatcgac caactagtgc agttggttgg cgacaaagcg actgtgcggc aaatgctggt
    12121 ttctcatctg ggccgcagct tccgccaggg gcggcacatc ctgcgcgtgc tctactatcg
    12181 tccgttgcgt aatctgctac ctggcagcgc attgcgccgt tccgaaaccc atattgcccg
    12241 gcaaatcttc agttcgctaa cgcgcataaa tgaggaaaat ggggaactgg aagcagacat
    12301 cgcccaccac tggcagcaaa tatcaccgct tcactggcgt ttctttttgc gtccaggagt
    12361 ccattttcat catggtcgtg aactggaaat ggacgacgtg atcgcctctt taaaacgaat
    12421 caatacgctg ccgctctatt cgcatattac ggacattgtg tcgccgacgc cctggacgct
    12481 ggatatccat ctcacgcagc cggaccgctg gttgccgtta ctgctggggc aagttccggc
    12541 gatgatcctg ccgtgcgaat gggaaaccct cagtaacttt gccagccatc ctattggcac
    12601 cggtccgtat gcggtgattc gcaacagcac caatcaactg aaaattcagg cattcgatga
    12661 cttcttcggt taccgggcat taatcgacga agttaacgtc tgggttctgc cggaaattgc
    12721 cgacgagcca gccggtggtc tgatgctaaa aggtccacag ggcgagaaaa aagagattga
    12781 aagccgcctg gaggaaggtt gctactattt actgttcgac agccgcaccc atcgcggggc
    12841 gaatcagcaa gtcagggact gggtaagcta tgtgctttct ccaactaatc tggtctattt
    12901 cgctgaggaa cagtaccagc aactgtggtt cccggcttat ggactgctcc cccgttggca
    12961 ccatgcccgc accataaaga gcgaaaaacc ggctggcctg gaaagcctca ccctgacctt
    13021 ttatcaggat cacagtgagc atcgggtgat tgccgggatc atgcagcaga ttctggcaag
    13081 tcaccaggtc acgctggaaa tcaaagagat cagctacgat cagtggcatg aaggagagat
    13141 cgagagcgat atctggctta atagcgccaa ctttacgctg ccgctggatt tttcgctgtt
    13201 cgcgcacttg tgcgaggtgc cgctgctcca acactgtatt ccgatcgact ggcaagccga
    13261 cgccgcccgc tggcgcaatg gcgaaatgaa cctggcgaac tggtgccagc aactggtcgc
    13321 cagtaaagca atggtgccgc ttatccacca ctggctgatc attcagggac aacgcagtat
    13381 gcgcggcctg cgtatgaata ccctcggctg gttcgatttt aaatcagcgt ggtttgcgcc
    13441 accggatcca tgattgctgg tagataacaa aatcactaca ctaacgccgt tctcaacggg
    13501 gtgccacgca aacgcgtgcg ctgagaaaat acccgtcgaa cctgatccgg ataacgccgg
    13561 cgaagggatt tgaggctcct tctcaagtcc tttgccactc ttttttgagg tgcaaagtgt
    13621 taaaaaaatg tcttcccctg ctgttgctgt gcacagcgcc cgttttcgct aaacccgttc
    13681 tgactgttta tacctacgat tccttcgccg ccgactgggg gcctggtcca aagatcaaaa
    13741 aagcttttga agccaactgt aactgcgaac tgaaactggt ggcgctggaa gatggcgttt
    13801 cgcttctcaa ccgtctacgg atggaaggca aaaatagtaa agccgatgtg gtgctggggc
    13861 tggataacaa tctgttagac gccgccagta aaaccggact atttgccaaa agcggtgtgg
    13921 cagcggatgc cgttaacgtt cccggcggct ggaataatga cactttcgtc ccgtttgatt
    13981 acggctattt cgccttcgtc tatgacaaga acaaactgaa aaacccgccg caaagcctga
    14041 aagaattggt tgagagcgat cagaactggc gggtgattta tcaggatccg cgtaccagca
    14101 cgccggggct gggtctgctg ttgtggatgc aaaaagtcta tggcgatgac gccccacaag
    14161 cctggcaaaa actggcgaag aaaaccgtca cggtcaccaa aggctggagc gaagcctacg
    14221 gcctgttttt aaaaggtgaa agcgatctgg tactgagtta caccacctct ccggcttatc
    14281 acattctcga agagaaaaaa gataactacg ccgccgcgaa ctttagcgaa ggtcactatc
    14341 tgcaggtgga agtcgccgcc cgcaccgctg ccagcaagca gccggagctg gcgcaaaaat
    14401 tcctccagtt tatggtttct ccggcttttc agaatgcgat cccaaccggt aactggatgt
    14461 atccggtggc aaatgtcacg ctgcctgccg ggtttgaaca attgaccaaa ccggccacca
    14521 cgctggagtt cacaccagct gaagtggcgg cacaacgtca ggcatggatt agcgaatggc
    14581 aacgcgccgt cagccgttaa ttcccggctg gttaattcca ggtgtaagcg ccgccacgct
    14641 ggtggtagcg gttgcgctcg cggcgtttct cgccctgtgg tggaacgcgc cgcagggtaa
    14701 ctgggtggca gtctggcagg acagctacct gtggcatgtg gtgcgcttct ccttctggca
    14761 ggcgtttctc tcggcgctac tctctgtcgt acccgcgata ttcctcgccc gcgccctcta
    14821 tcgcaggcgc tttccgggtc ggctggcgct gttgcgtctg tgcgcaatga ccttgatcct
    14881 cccggtgcta gttgctgttt tcggcattct tagcgtctat ggtcgtcagg gctggctggc
    14941 atcgctctgc caatcgctcg gtctggagtg gaccttttcg ccctacggcc tgcaaggcat
    15001 tttgctggcg cacgtatttt ttaatctgcc gatggcgagc cgcttattac tccaggcact
    15061 ggaaaacatt cccggcgaac aacgtcagct tgccgcccag cttgggatgc gcggctggca
    15121 ttttttccgc ttcgtcgaat ggccgtggct acggcgacaa atcccgccgg ttgccgcgct
    15181 tatctttatg ctctgtttcg ccagcttcgc caccgtgcta tcgctgggcg gcggtccgca
    15241 ggcaaccact atcgagctgg ctatttatca ggcgctgagt tacgactacg atcctgcccg
    15301 cgcggcgatg ctggcgctgc tccagatggt gtgttgcctt gggctggtgc tgttgagtca
    15361 gcgactaagt aaggccattg cgcccggcac cacgctgctg caaggctggc gcgatccgga
    15421 cgatcgtctg catagccgca tttgcgacac ggtgttaata gtgctggcgc tgctgctgtt
    15481 gctgccaccg ttgctggcgg tgatcgtcga tggggtaaat cgccagttgc cggaagtgct
    15541 ggcacaaccg gtgctgtggc aggcgctgtg gacctcgttg cgtattgcgc tggcggcagg
    15601 tgtattgtgc gtagtgctga ccatgatgct gctatggagc agtcgcgaac tgcgggcgcg
    15661 gcagaaaatg ctggcgggtc aggcgctgga gatgagcggc atgttgatcc tcgccatgcc
    15721 ggggattgtg ctggctaccg gcttcttttt actgctcaac aacactatcg gcctgccaca
    15781 atctgctgac ggcattgtga ttttcaccaa tgcgttaatg gcgatccctt atgcgctgaa
    15841 agtactggaa aacccgatgc gcgatatcac cgcccgctac agtatgttgt gtcagtcgct
    15901 gggcattgaa ggctggtcgc gcttaaaagt ggtcgagctg cgcgccctga aacgtccact
    15961 ggcgcaggcg ctggcttttg cctgcgtgct gtcgattggt gattttggcg tagtggcgtt
    16021 gttcggtaac gatgatttcc gtaccctgcc gttttatctc taccagcaaa ttggctccta
    16081 tcgcagccag gacggcgcgg tcaccgcgtt aattctgcta ctactctgtt ttctgctgtt
    16141 taccgtgatt gaaaaactac cggggcgaaa tgttaaaact gactgatatc acctggcttt
    16201 atcaccattt gccgatgcgt tttagcttaa cggtagaacg cggcgagcag gtggcaatcc
    16261 tcgggccaag cggcgcgggt aaaagtaccc tgctgaattt gattgccggt tttctgacgc
    16321 cagccagcgg ttcgctgact atcgatggtg ttgatcacac cacgacgccg ccgtcacgcc
    16381 gtccggtgtc gatgctgttt caggagaaca acctgttcag ccatctgacg gtcgcgcaga
    16441 acatcgggct ggggctaaat ccggggttga aactgaacgc ggcacagcag gagaaaatgc
    16501 acgcgattgc ccgtcagatg gggattgata atttaatggc gcggttaccg ggcgagcttt
    16561 ccggcggtca gcgacagcgc gtggcgttag cgcgttgtct ggtgcgcgaa cagccgattt
    16621 tattgctcga tgaaccgttc tctgcgctcg atccggcgtt acgtcaggag atgctgacgc
    16681 tggtgagcac gagctgccag cagcaaaaaa tgacgctatt gatggtgtcg cacagtgtgg
    16741 aagatgcggc gcggatcgcc acacgctcgg tggtggtcgc cgacgggcgc atcgcctggc
    16801 agggtaagac tgaagaattg ttgagcggta aggcgagtgc ttcggcaata ttggggatta
    16861 cgggttagta tgcactgtca gatgcggcgt gaacacctta tccgacctac ggacagtgcg
    16921 ttttgtaggc ctgataagac gcggtcagcg tcgcatgagg caacccgcac aagacaatcc
    16981 cctaaacccc aaccacttta cgcaaaatat cgatatacac cggcatcaac gggtggcgaa
    17041 ttaacaccac cagcgccacc acgccgatgg cagaaatcaa cggcgtcagc cacaacaaac
    17101 ggccgcgcga caaataatga cttaaacgat cggttgcttt accgctgcgc cataaccgcc
    17161 agcacagcca gccaccaacc cacaaaaata ccgccgtcgc cagcagcaac catttaaact
    17221 cgccgctctg cattccggct ggaatatcga tcgccgcgcc cgccagaatc ccaggcagga
    17281 agtaaaacgg tggccacagc aggcagccga taatattcgg cgtaatgaat tttgccaccg
    17341 gcagatccag cattcccgcc accattggca ccagcggacg cgtcgggcca acaaagcgac
    17401 cgaccagaat ggtgaacatg ctgtgttgat gcaacgcatg ttcagtttta tcgagtaatg
    17461 ctttgttttt cttcagaaat gaccagcgat gcaacggctt tttaaaacgc cagcccagcc
    17521 agaaggagat ccagtcgccc agcagacaac cgacaatccc tgccagccaa gcgtgccaga
    17581 aacttaactc gccgctgcca atcagcgctc ccagccccgc catcagcacc gtaccgggta
    17641 gaatcaaacc gaccagcgcc agcgactcca gaaaggccac caacaccacc gccatcaatg
    17701 aatacacggt agattgggta ataaagtgtt ccagcaatgc ttgcataatg tgcctgtcaa
    17761 atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga
    17821 ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca
    17881 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga
    17941 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc
    18001 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc
    18061 tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg
    18121 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacacga
    18181 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc
    18241 tcaagcagat tcatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg
    18301 atttgcccaa acaggtcgct gaaatacggc tggtgcgctt catccgggcg aaagaaaccc
    18361 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag
    18421 taaacccact ggtgatacca ttcgcgagcc tccggatgac aaccgtagtg atgaatctct
    18481 cctggcggga acagcaaaat atcacccggt cggcagacaa attctcgtcc ctgatttttc
    18541 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg
    18601 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg
    18661 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata
    18721 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc
    18781 gtcttttact ggctcttctc gctaacccaa ccggtaaccc cgcttattaa aagcattctg
    18841 taacaaagca ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc
    18901 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta
    18961 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca
    19021 tacccgtttt tttggatgga gtgaaacgat ggcgattgca attggcctcg attttggcag
    19081 tgattctgtg cgagctttgg cggtggactg cgccagcggt gaagagatcg ccaccagcgt
    19141 agagtggtat ccccgttggc aaaaagggca attttgtgat gccccgaata accagttccg
    19201 tcatcatccg cgtgactaca ttgagtcaat ggaagcggca ctgaaaaccg tgcttgcaga
    19261 gcttagcgtc gaacagcgcg cagctgtggt cgggattggc gttgacagta ccggctcgac
    19321 gcccgcaccg attgatgccg acggtaacgt gctggcgctg cgcccggagt ttgccgaaaa
    19381 cccgaacgcg atgttcgtat tgtggaaaga ccacactgcg gttgaagaag cggaagagat
    19441 tacccgtttg tgccacgcgc cgggcaacgt tgactactcc cgctatattg gcggtattta
    19501 ttccagcgaa tggttctggg caaaaatcct gcatgtgact cgccaggaca gcgccgtggc
    19561 gcaatctgcc gcatcgtgga ttgagctgtg cgactgggtg ccagctctgc tttccggtac
    19621 cacccgcccg caggatattc gtcgcggacg ttgcagcgcc gggcataaat ctctgtggca
    19681 cgaaagctgg ggcggcttgc cgccagccag tttctttgat gagctggacc cgatcctcaa
    19741 tcgccatttg ccttccccgc tgttcactga cacctggact gccgatattc cggtgggcac
    19801 cttatgcccg gaatgggcgc agcgtctcgg cctgcctgaa agcgtggtga tttccggcgg
    19861 cgcgtttgac tgccatatgg gcgcagttgg cgcaggcgca cagcctaacg cactggtaaa
    19921 agttatcggt acttccacct gcgacattct gattgccgac aaacagagcg ttggcgagcg
    19981 ggcagttaaa ggtatttgcg gtcaggttga tggcagcgtg gtgcctggat ttatcggtct
    20041 ggaagcaggc caatcggcgt ttggtgatat ctacgcctgg tttggtcgcg tactcggctg
    20101 gccgctggaa cagcttgccg cccagcatcc ggaactgaaa gcgcaaatca acgccagcca
    20161 gaaacaactg cttccggcgc tgaccgaagc atgggccaaa aatccgtctc tggatcacct
    20221 gccggtggtg ctcgactggt ttaacggtcg tcgcacgcca aacgctaacc aacgcctgaa
    20281 aggggtgatt accgatctta acctcgctac cgacactccg ctgctgttcg gcggtttgat
    20341 tgctgccacc gcctttggcg cacgcgcaat catggagtgc tttaccgatc aggggatcgc
    20401 cgtcaataac gtgatggcgc tgggcggcat cgcgcggaaa aaccaggtca ttatgcaggc
    20461 ctgctgcgac gtgctgaatc gcccgctgca aattgttgcc tctgaccagt gctgtgcgct
    20521 cggtgcggcg atttttgctg ccgtcgccgc gaaagtgcac gcagacatcc catcagccca
    20581 gcaaaaaatg gccagtgcgg tagagaaaac cctgcaaccg cgcagcgaac aggcacaacg
    20641 ctttgaacag ctttatcgcc gctatcagca atgggcgatg agcgccgaac aacactatct
    20701 tccaacttcc gccccggcac aggctgccca ggccgttgcg actctataag gacacgataa
    20761 tggcgatttt tgataattat gaagtgtggt ttgtcattgg cagccagcat ctgtatggcc
    20821 cggaaaccct gcgtcaggtc acccaacatg ccgagcacgt tgttaatgcg ctgaatacgg
    20881 aagcgaaact gccctacaaa ctggtgttga aaccgctggg caccacgccg gatgaaatca
    20941 ccgctatttg ccgcgacgcg aattacgacg atcgttgcgc tggtctggtg gtgtggctgc
    21001 acaccttctc cccggccaaa atgtggatca acggcctgac catgctcaac aaaccgttgc
    21061 tgcaattcca cacccagttc aacgcggcgc tgccgtggga cagtatcgat atggacttta
    21121 tgaacctgaa ccagactgca catggcggtc gcgagttcgg cttcattggc gcgcgtatgc
    21181 gtcagcaaca tgccgtcgtt accggtcact ggcaggataa acaagcccat gagcgtatcg
    21241 gctcctggat gcgtcaggcg gtctctaaac aggatacccg tcatctgaaa gtctgccgat
    21301 ttggcgataa catgcgtgaa gtggcggtca ccgatggcga taaagttgcc gcacagatca
    21361 agttcggttt ctccgtcaat acctgggcgg ttggctatct ggtgcaggtg gtgaactcca
    21421 tcagcgacgg cgatgttaac gcgctggtcg atgagtacga aagctgctac accatgacgc
    21481 ctgccacaca aatccacggc gaaaaacgac agaacgtgct ggaagcggcg cgtattgagc
    21541 tggggatgaa gcgtttcctg gaacaaggtg gcttccacgc gttcaccacc acctttgaag
    21601 atttgcacgg tctgaaacag cttccaggtc tgcccgtaca gcgtctgatg cagcagggtt
    21661 acggctttgc gggcgaaggc gactggaaaa ccgccgccct gcttcgcatc atgaaggtga
    21721 tgtcaaccgg tctgcagggc ggcacctcct ttatggagga ctacacctat cacttcgaga
    21781 aaggtaatga cctggtgctc ggctcccata tgctggaagt ctgcccgtcg attgccgtag
    21841 aagagaaacc gatcctcgac gttcagcatc tcggtattgg tggtaaggac gatcctgccc
    21901 gactgatctt caatacccaa accggtccag cgattgtcgc cagcctgatt gatctcggcg
    21961 atcgttaccg tctgctggtt aactgtatcg acacggtgaa aacaccgcac tccctgccga
    22021 aactgccggt ggcgaatgca ctgtggaaag cgcaaccgga tctgccaact gcttccgaag
    22081 cgtggatcct cgctggtggc gcgcaccata ccgtcttcag ccatgcgctg aacctcaacg
    22141 atatgcgcca gttcgccgag atgcacgaca ttgaaatcac ggtgattgat aacgacaccc
    22201 gcctgccagc gtttaaagac gcactgcgct ggaacgaagt gtattacgga tttcgtcgct
    22261 aagtagccac atcaggtatg taacgcctga tgcgacgctg acgcgtctta tctggcctac
    22321 acgctgcgat tttgtaggcc ggataagcaa agcgcatccg gcattcaacg cctgatgcga
    22381 cgctggcgcg tcttatcagg cctacgcgct gcgattttgt agaccggata agcgaagcgc
    22441 atccggcacg aaggagtcaa catgttaaaa gatctcaaac gccaggtatt agaagccaac
    22501 ctggcgctgc caaaacacaa cctggtcacg ctcacatggg gcaacgtcag cgccgttgat
    22561 cgcgagcgcg gcgtctttgt gatcaaacct tccggcgtcg attacagcgt catgaccgct
    22621 gacgatatgg tcgtggttag catcgaaacc ggtgaagtgg ttgaaggtac gaaaaagccc
    22681 tcctccgaca cgccaactca ccggctgctc tatcaggcat tcccctccat tggcggcatt
    22741 gtgcatacgc actcgcgcca cgccaccatc tgggcgcagg cgggtcagtc gattccagca
    22801 accggcacca cccacgccga ctatttctac ggcaccattc cctgcacccg caaaatgacc
    22861 gacgcagaaa tcaacggcga atatgagtgg gaaaccggta acgtcatcgt agaaaccttt
    22921 gaaaaacagg gtatcgatgc agcgcaaatg cccggcgttc tggtccattc ccacggcccg
    22981 tttgcatggg gcaaaaatgc cgaagatgcg gtgcataacg ccatcgtgct ggaagaggtc
    23041 gcttatatgg ggatattctg ccgtcagtta gcgccgcagt taccggatat gcagcaaacg
    23101 ctgctggata aacactatct gcgtaagcat ggcacgaagg catattacgg gcagtaatga
    23161 ctgtataaaa ccacagccaa tcaaacgaaa ccaggctata ctcaagcctg gttttttgat
    23221 ggattttcag cgtggcgcag gcaggtttta tcttaacccg acactggcgg gacaccccgc
    23281 aagggacaga agtctccttc tggctggcga cggacaacgg gccgttgcag gttacgcttg
    23341 caccgcaaga gtccgtggcg tttattcacg ccgatcaggt tccccgcgct cagcatattt
    23401 tgcagggtga acaaggcttt cgtctgacac cgctggcgtt aaaggatttt caccgccagc
    23461 cggtgtatgg cctttactgt cgcgcccatc gccaattgat gaattacgaa aagcgtctgc
    23521 gtgaaggtgg cgttaccgtc tacgaggccg atgtgcgccc gccagaacgc tatctgatgg
    23581 agcggtttat cacctcaccg gtgtgggtcg agggtgatat gcacaatggc actatcgtta
    23641 atgcccgtct gaaaccgcat cccgactatc gtccgccgct caagtgggtt tctatagata
    23701 ttgaaaccac ccgccacggt gagctgtact gcatcggcct ggaaggctgc gggcagcgca
    23761 tcgtttatat gctggggccg gagaatggcg acgcctcctc gcttgatttc gaactggaat
    23821 acgtcgccag ccgcccgctg ctgctggaaa aactcaacgc ctggtttgca aaccacgatc
    23881 ccgatgtgat catcggctgg aacgtggtgc agttcgatct gcgaatgctg caaaaacatg
    23941 ccgagcgtta ccgtcttccg ctgcgtcttg ggcgcgataa tagcgagctg gagtggcgcg
    24001 agcacggctt taaaaacggc gtcttttttg cccaggctaa aggtcggcta attatcgacg
    24061 gtatcgaggc gctgaaatcc gcgttctgga atttctcttc attctcgctg gaaactgtcg
    24121 ctcaggagct attaggcgaa ggaaaatcta tcgataaccc gtgggatcga atggacgaaa
    24181 ttgaccgccg tttcgccgaa gataaacctg cgctggcaac ttataacctg aaagattgcg
    24241 agctggtgac gcagatcttc cacaaaactg aaatcatgcc atttttactc gaacgagcaa
    24301 cggtgaacgg cctgccggtg gatcggcacg gcggttcggt ggcagcgttt ggtcatctct
    24361 attttccgcg aatgcatcgc gctggttatg tcgcgcctaa tctcggcgaa gtgccgccgc
    24421 acgccagccc tggcggctac gtgatggatt cacggccagg gctttatgat tcagtgctgg
    24481 tgctggacta taaaagcctg tacccgtcga tcatccgcac ctttctgatt gatcccgtcg
    24541 ggctggttga aggcatggcg caacctgatc cagagcacag taccgaaggt tttctcgatg
    24601 cctggttctc acgggagaaa cattgcctgc cggagattgt gactaacatc tggcacgggc
    24661 gcgataaagc caaacgtcag ggtaataagc cgctttcgca ggcgctgaag atcattatga
    24721 atgcctttta tggcgtgctc ggcaccaccg cctgccgctt cttcgatccg cgcctggcat
    24781 cgtcgatcac catgcgtggt catcagatca tgcggcagac caaagcattg attgaagcgc
    24841 agggctacga cattatctac ggagataccg actcgacttt tgtctggcta aaaggtgcac
    24901 attcggaaga agaggcggcg aaaatcggtc gtgcactggt gcagcacgtt aacgcctggt
    24961 gggcggaaac gctgcaaaaa caacggctga ccagcgcatt agaactggag tatgaaactc
    25021 atttctgccg ttttcttata ccaaccattc gcggagctga taccggcagt aaaaagcgct
    25081 acgccgggct gattcaggag ggcgacaagc agcggatggt gtttaaaggg ctggaaaccg
    25141 tgcgcaccga ctggacgccg ctggcccagc agtttcagca ggagctgtac ctgcgtatct
    25201 tccgcaacga gccatatcag gagtacgtcc gccaaaccat cgacaaactg atggcgggtg
    25261 aactggatgc gcggctggtt taccgtaaac gccttcgccg tccgctgagc gaatatcagc
    25321 gtaatgttcc gcctcatgta cgcgccgctc gccttgccga tgaagaaaat caaaagcgcg
    25381 gtcgcccctt gcaatatcag aatcgcggca ccattaagta cgtatggacc accaacggcc
    25441 cggagccgct ggactaccaa cgttcaccac tggattacga acactatctg acccgccagc
    25501 tacaacccgt ggcggaggga atactccctt ttattgagga taattttgct acacttatga
    25561 ccgggcaact tgggctattt tgagcaaaaa aaagagttcg ccagatacca ttttgatgcg
    25621 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    25681 ccactacctc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    25741 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    25801 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    25861 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    25921 tgataccatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    25981 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    26041 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    26101 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    26161 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    26221 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    26281 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    26341 cgctgctgaa cgtgtgctga ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    26401 aatgctgcgc cgtttcaacc tgcgctttgc tctgtttgat gatgagcgtt atgccgaagc
    26461 tcagcacgat gcttacaacc cgtttgacac cgaacagctg gtgatttgct cgctggattt
    26521 tgcccgtcgt agcaaacagc gcttggaaca tctctgtgaa gccgaatggg acctgctggt
    26581 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    26641 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    26701 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    26761 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    26821 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    26881 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    26941 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    27001 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    27061 gccgctaccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    27121 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    27181 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    27241 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    27301 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    27361 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    27421 ggtactgctg tgctcagaaa tcggttctga aggacgtaac tttcagttcg ccagtcacat
    27481 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    27541 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    27601 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    27661 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccgga
    27721 tcaaaccgaa ggctttgacg atctgatcaa aagctgccgc gagcaacatg aagcgctgaa
    27781 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    27841 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    27901 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    27961 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    28021 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    28081 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg ataccggtag
    28141 cagcacgatt tcactgttaa aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    28201 gatttatgtg gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    28261 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    28321 tgaaaccttt aaccgccagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    28381 cgccgtgcag caggatgttc acgctatcct tcaactgggt gaagcgcaga tcgagaaatc
    28441 tgcccgtgca ttgattgatg cagcgcgtaa cgaagccgac gaaaaactgt ctgccgagct
    28501 gtctcgtctg gaagctctgc gtgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    28561 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggtt ggcgtctgga
    28621 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    28681 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca tattatggtg
    28741 gtcaacaagc cgagcggttt gttgtcagtg ccgggtcgtc tggaagagca caaagacagc
    28801 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    28861 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    28921 cagttccgcg agcgcgagcc gaaaaagcag tatgtggccc gcgtctgggg gcatccatcc
    28981 cccgcagaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    29041 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    29101 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcaattgcgt
    29161 gtgcatatgc tggcgctggg gcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    29221 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    29281 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    29341 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    29401 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    29461 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    29521 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    29581 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    29641 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    29701 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    29761 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    29821 agtgaaccat cagcaaacgc cgcctgaatc tggatctcca gaaacatacg aattaagtca
    29881 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    29941 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    30001 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    30061 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    30121 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    30181 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    30241 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    30301 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    30361 ttgcattatc atacctgtca ttcaatgccg tgcctaacac cactgacgct attcggacag
    30421 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    30481 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    30541 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    30601 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    30661 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    30721 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    30781 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    30841 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    30901 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    30961 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    31021 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    31081 ccgcgaagaa caagttgctg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    31141 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    31201 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    31261 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    31321 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    31381 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    31441 ttggttattc tactggaacc actccggggt catggatcag gtgtggcgtt tcaacgtcga
    31501 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    31561 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    31621 caccgtttca accaagcagt tccaggtttt cagcgaacag aacaccagta gctactcggc
    31681 agagccgcag ttagacgtta attactacca gaatgatgtt ggtccgtttg atacgcgtat
    31741 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    31801 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    31861 agcgaagttt ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    31921 cacgaccaag ctggacgaat ccgttaaccg cgtaatgccg caattcaaag ttgacggcaa
    31981 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    32041 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    32101 gtctctgctg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    32161 ccgtattgcc tccgctaacc aagtgacgac cggtgtcaca tctcgcatat atgatgatgc
    32221 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    32281 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    32341 cgacacctac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    32401 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    32461 tctggtacag ctgaattacc attacgccag cccggaatat attcaggcta cgctgcctaa
    32521 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    32581 caggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    32641 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    32701 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    32761 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    32821 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    32881 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    32941 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    33001 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    33061 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    33121 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    33181 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    33241 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacactt
    33301 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    33361 gtcgcatcac catcctgcca caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    33421 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    33481 cctctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    33541 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    33601 gcggccagat ggggtggggc cgtattcagg agttgccggg gatcttcgcc caggcattaa
    33661 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    33721 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    33781 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgc gtgaaactgg
    33841 agcaaattgc tgctgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    33901 tctcgcagga tccaggttct gctaaccagg gcggtgatct cggctgggct acaccagata
    33961 ttttcgatcc agccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    34021 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    34081 aaaccgacgc tgcgcagaaa gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    34141 aagaagcagc aagctggatg caggaacaac gtgccagcgc ctacgttaaa atcctgagca
    34201 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    34261 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    34321 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    34381 ttcccccaac tcccctgcac aaccgcaaac tacgggcaca ttaacgctac ttcctgtcgc
    34441 gctacgtgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    34501 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    34561 ccccgtgcat aaaggcgtca ttaacgacgc aagcattccg tttaccggtc ataccgagtt
    34621 tttcgaagag cgttcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    34681 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    34741 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    34801 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    34861 tacggaagag atagacacca ttattccggt gctcgacgag ctgcgggcgc aggggatgaa
    34921 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    34981 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    35041 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    35101 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    35161 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    35221 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    35281 tctgccatta acccgcaaaa gggccaggca atggtcgaaa tcggccccgg tctggcggca
    35341 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    35401 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    35461 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    35521 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    35581 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    35641 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    35701 atcccggtac tggaagttcc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    35761 gtcgtgcgcc tggtccctca tgcaacgatg cctcacccgg ttaaagatat tcgcgtgttg
    35821 agccgcatca ccaccgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    35881 aacctgttta gcgtcgaggt gttaaccgga atggggatcg acccggcgat gcgagcggaa
    35941 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    36001 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    36061 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    36121 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    36181 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    36241 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    36301 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    36361 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    36421 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccccg
    36481 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cggcccgggt tcgctggatg
    36541 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    36601 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    36661 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctctgc
    36721 tgcaaatcga cgaagagaaa aagctggtga tggcccacgc agggatcacg ccgcagtggg
    36781 atctgcagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctgtcg agtgactcct
    36841 atcccttctt tcttgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    36901 gggggctggg aagactgcgt tttatcacca acgcctttac ccgtatgcgt ttttgcttcc
    36961 cgaacggtca actggatatg tacagcaaag aatcgccgga agaagcccct gccccactga
    37021 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    37081 gggcatcgct ggaaggcaaa ggtacgccgg aaggtattta cgcgctggat accggctgct
    37141 gctggggtgg gtcattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    37201 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatacaggaa
    37261 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaactt
    37321 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatcg gcgtcgtgga
    37381 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    37441 ccacttctgc gtcgatatgc gtcagataca gtttttgcgc ttttggcagg aactgctcat
    37501 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcaatggctt
    37561 catccaccga tttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    37621 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    37681 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    37741 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    37801 agatttcccg ataaaaaaat tgtcgccact atacgtaaag cgtaaaccgt cgtcgactgg
    37861 tgcgaggatg atgttgagga aaattttata ttctgctggc gagtccacgc tctccccctg
    37921 gactcgccgc attacaatga aacaaaaaca aacagttagc tgtaaagtgt gatttacgtc
    37981 actctttatt aagatgaggg tttcgtttcc ggttcatccg ccatgttgcc ggtatgttta
    38041 ccttcttctg ttccctgcca gccatgtcgt tgaattaatg acagatgttc gcggtcctcg
    38101 gtgatgatct cacttaacat cgcgctggtg cgtttataga ccgccgcgcg ggctttggtg
    38161 tcattctcaa ccattgccat ctcttccacc atctgaatat taaagcggcg gaacacatcg
    38221 gcacgttctc gcgcttcata cggccccaga cctaaacttt ccagcgccag acgcccggtt
    38281 ttcagcgcac cttcaaaggt ttcacgctcc ggcttttcaa cgcctgcctg acgcaaacga
    38341 atgtagtgat cgacatcgcg ggcgcgggca ataatctgca aatgcgggaa atgttctttc
    38401 accatctccg tcagttgcag gttggtttgc ggatcgtcaa tagcgttaat cagcacttcc
    38461 gctttcgccg ctccggcaga ttccagtaaa tccatccgcg tggcatcgcc ataaaacact
    38521 ttcataccaa atttacgcag cgtttcgata tggtccggat cgtgatcgag caccaccatt
    38581 ttcaccccgc tggagagcag taaacgtccg gtaatctgtc caaaacgacc gaatccggcg
    38641 ataatcacgc gcggctgttc ttcatcgatc tcatcggctt cacgcgcctc ctccgtagaa
    38701 gattgctcaa ggcgattgag gatcaccagc agaatcggcg ttgctgccat cgacagcgcc
    38761 accgccaggg tcagcgattt cgcccactcc ggctccagca cattcgccat ctgcgccgca
    38821 ccaaatacca caaaggcaaa ctcactgccc tgccctaaca acaccgcaaa ccagcgacgc
    38881 tgtttatttg gcacctgcaa cggtcgggca atcagccaca gcatggcgat tttgatgatg
    38941 aggaaaccga gcagcaaaat gacaatgcgc aatgggtttt caatcagcgt gccaaagtct
    39001 atcgacatgc caacaccgat gaaaaacagc cccaacagca aacctttaaa cggttcgata
    39061 tcgctttcca acgcatgacg gtattccgag cttgccagca gtacgcccgc cagaaacgcg
    39121 cccatcgcca tcgacaagcc gacctcttcc agcagcaaac caaagccaaa cacaaggaat
    39181 aacgccacgg cactaaacac ttcccgcaag ccagagcggg caacaaaacg cagcgccgga
    39241 cgcgtaacat agcgccccag caataccacc agtaccagcg cacccgccac ttttaacgcc
    39301 gagagagcaa atgcgcccat cgtcgtcgag gcactgctcg ccgccagtag cgggatcatc
    39361 gccaccagcg ggattgccgc gatatcctgg aacagcagca ctgcaaaggc actgcggccc
    39421 atttgcgtca ccatcagatt gcgttcattc atcgcctgca tggcaatcgc cgttgatgag
    39481 agcgccagcg tcataccgat caattccgcg acctgccagc gcagcccaag taacatacag
    39541 aacagcccca gcaggccacc gcaaatcacc atctgcaatg cgccaccgcc gaacactgcc
    39601 gcacgcagct tccacagcct ttgtggatcg agttcgaggc cgataataaa cagcatcagc
    39661 accaccccaa tctcggcaaa gtgcagaata gattcggcat cggtcaccag tcgcagcccc
    39721 cacgggccaa taatgcagcc ggcgatcagg tagccaagta ccgatcccag accaagacgt
    39781 accgcaatgg gtacaatcag cgccgccgat ccgaggtaga tcagcgcctg aaccagcgta
    39841 tgactatcca tgatgggcct cctgccattc cagcagacgt tgcttatagt ggcgcgcctg
    39901 cccttcgagg gtttcgtcgt cacaaataaa ggtgcagtgc atggcaaacg gtggcagcca
    39961 gttcagcccg cagtagattg ccgtcgcctg tagcggctgc gacagcacat caaagcccgg
    40021 atgcgcacca atttcaaaat ggctttcccc gccgccggtc gtcaccgccc acagcaaatg
    40081 tttgccatgc agcgccgtgc cgccgtgacc gtaagcccag ccgtgcgaca aaactttatc
    40141 gatccaaagt ttaagaagcg gaggaatgct gtaccactgc atcgggtgct gccagacgat
    40201 cagatcggcg cgagagagcg cctcctgctc ggcggcaata tcgatattga agtcaggata
    40261 gagttgataa agagagcgaa tttcgacgcc ttccagcgtc cttgcctgtt caagcatccg
    40321 tttattcgca tgggaatgat gcggatacgg atgcgcataa atgatgagga tcatagttat
    40381 tagcccgcgt gtttacgcct ttgttttccc acaaagtgta gtcatatatt cacgcggctt
    40441 atagtcaata atattgattc agaaaagaga gaaatctgaa caaataatcc cattctctgc
    40501 ccgcggatcg tcatattgct ggtctgcgcc agtgacattc ctcgagtctc cggggcaaac
    40561 gctacggaaa tcaacaagcc aaacagcgag atacccgccc ccattagcat cgtgttactg
    40621 ataccgtaat tattgataaa tatcggcagt gcccaggtcg aaacaatggt gccaatacga
    40681 cttaaggaca taatcacgcc cacggcagag gcgcggatat ccgtcgggaa gagttcatta
    40741 ggatagagcc actgcaaatt acccggcccg ccagagaaaa aggcatacac cgcaaaggcc
    40801 atcactacca gccagatccc catatccggg attagcccca aaaccgccaa cgccagcgtc
    40861 atcatggcaa agctgccaat caacaatgga cgccgtccgg cagtgtttag ccacagcatc
    40921 ggcggaatac agccgagcat aaagaacagg ctaatcacca cattccctag tgccgcgttt
    40981 ttgccaaccc ccaatcccaa cgaaccaacg atttgcgggc caaaggtgta aatggcgaac
    41041 attgggatca cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg aaaatggcgg
    41101 cgattaaaca gatcgcgaaa acgggtttgc tgcggctgct cttcatcgaa agccaccggt
    41161 tcgccaaaca gttttatcat catctcttcg cactctttta ctcgcccttt gcgtaataac
    41221 cagcggggag attctggcag ttcgaatcga ccaatcaaaa tcaacaaaca agggatcgcc
    41281 gcgctaccca gcatccagcg ccaaccacct tccacatcat aaagccagta gccgaccaga
    41341 tcggcgcagg tcgcgccgac ataccacatc gcggcaataa agctgatgga aaacgcccgc
    41401 tgacgggtac tggagaactc ggtgatcatc gaagtggcga tgggataatc tgcaccgatg
    41461 acaatgccga tgagtacccg catcaccaac agttcgacgg gggatgaaac acacatcgtc
    41521 gccaccgata tcacgccgat ggcgatgata tcaatgagga acattttgcg ccgtccgact
    41581 ttatcggaga tatagccaaa cagcgatgtg ccaacgaaca gcccggcgag cgttcccgcg
    41641 cccagcaagc caatccagtc agcgtccagt ttcagcgccg gagtcagttg ctccagcgcc
    41701 acgccaatca ttaccagtac ataaccatcc agaaacggac caccgcttcc ccacagcaaa
    41761 atgcggcggt gaatagagga gaatttgaga tcgtcaaagt ttctggacgg ttgcatgggt
    41821 tattcctgtt tttttgcaat gagagtcagt acggcgttac gtatcaaacc aacatcagcc
    41881 gtaacggaac tccacaccaa aggtgccgcg cgggtattcc cactgttcca gcgccgaccc
    41941 cagccccaga atgcgacagg tgccgcactc cagacatccg gcgtaatcga agcgcacact
    42001 gccgtcatcc tgcttcttgt acagacctgc ggggcacgct ttcaccagca gctccagcac
    42061 ctgtttatca gcatcggcct tcacaacaat gtgcggatgc tcttcatcga cattgaattt
    42121 attgacgccc agtttgacgt ccacattgac gggagaagtc ataaaacggt cactcctttc
    42181 atgccatcct tgatcagatt gatgaagccc actttcttgc cgtggcggag gattttcttg
    42241 cgcatcagtt ccggcgcact gccgtcaatg gtgaacaggt cacgcgccac gcccaccgcc
    42301 agttccgggt tgccgctaaa catgcgtggg ttatcaagga acgccggtag tttctggtac
    42361 atgcgcatat cgcgcagcgg gccactctcc agatgctgac gatattccgc gagtttttgc
    42421 ttactgaaat cgtcgctttt catcgctgaa agcacggttt ttgctgcagc ttccccggcg
    42481 gcaatcgcca gatccatacc gcgaatggta aaaccgaggt tcatacacat tccggcggca
    42541 tcaccggcaa tcaatacgcc gtcaccgacc agctccggca gcatgttgat gcctgcttcc
    42601 ggcactacgt gagcggaata ttccaccagc ttgccgcccg cgatcagcgg tgcaacggcc
    42661 ggatgctgtt tgaaatcttc cagcatttgc ggcaccgatt ttttcgcgtc atgcagatga
    42721 tgcagaccac aaaccagccc cagcgacagg gtattttcat tggtataaag gaagccgccg
    42781 cccatcaggc catcggtggg tgatcccgca aacagacaag ccgcgccctg attaccctgc
    42841 aactgaaaac ggtcttcgat taccgacttc ggtaactcga tcagttcctt cacgccaacc
    42901 gccacatccg tcggtttaac gcgttttgcc atccccaact tttcggcaag gatggagttc
    42961 accccatcag caaggagcac cgttttcgct tcaatcacat caccatcggc ttctacaccg
    43021 acgactttgc catcgcgctg tacgaggtta tcgacgcgga tcccggtaat taactgcgcg
    43081 cccgcttctt cggcctgctc catcaaccag gcatcaaatt tactgcgcaa aacggagtaa
    43141 gaacgctggg atggcgaggt ttcgtcaccg ttgcagtagt ccatagtcat cgctgacttt
    43201 tccgtcataa acgcgagttt ttcatgggtg atcaggcgtt ctacgggggc ggaatcggcg
    43261 aaacctggaa taatgtgttc cagactgtgg gcatagagac gcccgccagt aacgttcttg
    43321 gcacctgcgg aattgccacg ctcgataact aacacttgcg ccccttcgcg ggcgagcacc
    43381 agtgcggcaa ccgaaccggc aagccctgca ccgacgatga tggcgtcaaa gatatcttcg
    43441 gacataagag ctccctgtca gcggcagagc cgtaatgcag ccctgccaga gtggatcaac
    43501 gcgctaaagc tgcggtcagt gccggaagga tcttcacggc atcgccaaca atgccgtaat
    43561 ccgcgtactg gaagatcggc gcatttttat ctttattgat ggcgaaaatg gtttgcgacg
    43621 cgttagcgcc aaccatgtgc tggatctacc cggagatccc caccgccagg tacagttcag
    43681 gtttcagcat caggttggag ataccgacat agcgttcgtg ctccatccat ttttcgtttt
    43741 ctgccaccgg acgagaacag gccaactccg cacctatcgc cttgcaaagc tgttctgcca
    43801 gcgcaatgtt ctctttgctg ccaatgccgc gaccgacgct gaccaccaga cgggctttgt
    43861 cgagatcgac gctgttgctc tggcgcgcct gggttgccgt gcgggtaatc gccacagccg
    43921 gagcctgcca ctccacggtg tgcgtttcgc cagtgcgtga cgcgtctggc tgagccgcat
    43981 cgaacgtgcc gctgctgatg gtcagtaccg catacggcgt ggcaatgcgt tcttcgccaa
    44041 tcgccagacc gccgtaaacc atgtgtttca ctgttgcttt accgtcctgt acgctgacgg
    44101 tgctggcatc gttagacacc gccgctttaa ggcgataacc cagttttgcc gccagtaatt
    44161 tgccgcgacg ggtgtttggc aacagcacca gaccgtcagc gccgtgctgg cgaatagtgt
    44221 cagccatgac accggcgtaa tcttcgatca tccgatcgtc cggtttgccg tttaatttcc
    44281 agacatgatt agcgccgagc tggattgcct gtgcgccgtc ggcatcattg aggacaaagg
    44341 cattgatttg attagctaaa gcctgcgcac cgttcatcag ttccggcaga cgagaggggg
    44401 tatcgctgaa tacccagact tgagaaaacg tgttcatagc atcccctgta attaaatgac
    44461 tttgcgaaga ttttcagcaa atgcggcgat ctgttcttcg ccgtcgcctt caatcacgat
    44521 gcgctgacgc tcgcgctgtt tcggcgcggc aacctgttgt tcagaccagg cgtccacggc
    44581 gttaaaacca atatccgccg ccgaccatac ctggactggc tttttcgccg cgccgagaat
    44641 ggctttcatc gaaggaattt gtggggagtt gatatcagtg gaaacagcaa caaccgcagg
    44701 cagcggaatg cttaaggttt cggtttcatc ttccagttcg cgctcaacgg tgagggtatc
    44761 tgccgtcagg gagataattt tgctgacgcc gttaactgcc ggaatattga ggatttcgcc
    44821 caccagcaga ccaacctgct gggcataaag gtcggaagaa ccatcgccac agaggatcag
    44881 atcaaagcct gctttctggg cggctgcagc cagtgcgctc gccgtttgtt gcggcagtgc
    44941 ctgctcgaac tggtcatcaa tcaccacaat cagttcatcc gggccgcgcg atagcacatc
    45001 tttacgccct ttggcgttgg tcagggcttt accgcccaca cttaaggctg tcacctgcgc
    45061 ctctgctgcc tgttgcttta gctggcaagc cgcttcaata gcgttgagat cgtattggct
    45121 tattttggca tcggctttgc tgaagtctaa tgaaccatca gcattattga ccgcaatatc
    45181 ctgttcatca ggcacgcact tatagcaagt aataatcttc attgcatctc cagaaatcat
    45241 gaaggaaacg tcgttcatat cggtggtgtt aattacagat atcacgccct ttaatttgga
    45301 aataaaactt taatcaccaa tattgaaagt cagatgctat ttttttaccc acaaaagaat
    45361 ataacaaact gattaataac caaaattaac gaaaaacgcc ctctgttcac tcgaaacaaa
    45421 aatgtgatac caatcacaga atacagctta ttgaataccc attatgagtt agccattaac
    45481 gcgtccacga tgttaataat aattatatta aatgttaaca aaaataaaac aaacgggaac
    45541 gcaaaataaa tattcgtttt cacagtggaa ttaactcatg aagaatgaaa agagaaaaac
    45601 gggaatagaa ccgaaggttt tctttccgcc attaataatc gtcggcatac tttgttggct
    45661 tacagtcaga gatctggatg cggcgaatgt cgttattaat gctgtattca gttacgtcac
    45721 caatgtatgg ggatgggcat ttgaatggta tatggtggtg atgcttttcg gttggttctg
    45781 gctggtgttt ggcccgtatg ccaaaaagcg tttaggtaac gaaccgccag aatttagcac
    45841 cgccagttgg atctttatga tgttcgactc ctgtacgtct gctgccgtac tgttctgggg
    45901 atcgattgag atctactact acatctccac cccgccgttt ggcttagaac cgaactcgac
    45961 aggagcgaaa gagttggggc tggcttacag cttgttccac tggggacctc tgccgtgggc
    46021 cacttacagc ttcctttcag tcgccttcgc ttacttcttc tttgtccgca aaatggaagt
    46081 gattcgcccc agctcgacac tggtgccgct ggcaggtgaa aaacacgcca aagggttgtt
    46141 cggtaccatc gtcgacaact tctatctcgt cgccttgatc ttcgcgatgg gtaccagtct
    46201 gggccttgcc acgccgctgg tgaccgagtg tatgcaatgg ttgtttggca ttccgcatac
    46261 cctgcaactg gacgctatca tcattacctg ctggattatc ctcaacgcca tttgcgtcgc
    46321 ttgcggtctg caaaaagggg tacgtatcgc cagtgacgtg cgtagttacc tgagcttcct
    46381 gatgctgggt tgggtgttca tcgtcagcgg tgccagcttc atcatgaact acttcaccga
    46441 ttcggtgggg atgttgctga tgtatctgcc gcgcatgttg ttctataccg atcccatcgc
    46501 taaaggcggc ttcccgcagg gctggaccgt gttctactgg gcatggtggg tgatttacgc
    46561 catccagatg agtatcttcc tcgcccgcat ctcccgtggt cgtactgtgc gtgaactgtg
    46621 cttcggcatg gtgatggggc tgacagcatc cacctggatc ctgtggaccg tgctcggtag
    46681 taacactctg ctgttgatgg ataaaaacat catcaacatt ccaaatctga tcgaacagta
    46741 cggtgtggcg cgcgccatca tcgaaacctg ggccgctctg ccactcagca ccgccaccat
    46801 gtggggcttc ttcatcctct gctttattgc caccgttacg ctggttaacg cctgctctta
    46861 taccctggcg atgtctactt gccgcgaagt acgcgatggt gaagaaccac cgctgctggt
    46921 gcgtatcggt tggtcaattc tggttggcat tatcggtatt gttctgctgg cgctcggcgg
    46981 cctgaaaccg attcaaaccg ccattatcgc cggaggatgc ccgctgttct tcgtcaacat
    47041 tatggtgacg ctctccttta ttaaagacgc gaaacagaac tggaaagatt aattaacccc
    47101 caaaatatca agaggttgaa agatggattt taatttaaat gatgagcagg aactgtttgt
    47161 cgccggtatc cgcgaactga tggccagcga aaactgggag gcctattttg ccgagtgcga
    47221 ccgtgacagc gtctacccgg aacgttttgt caaagcactg gcggatatgg gtatcgacag
    47281 tctgctgatc cctgaagagc acggtggtct ggacgcgggg tttgttactc tcgccgccgt
    47341 gtggatggag ctgggacgtc tgggggcacc aacctatgta ctgtaccagc tgccgggcgg
    47401 gttcaatacc ttcctgcgcg aaggcacaca agagcagatc gacaagatta tggctttccg
    47461 cggcaccggt aagcagatgt ggaactcagc gattaccgaa ccgggcgcgg gctccgacgt
    47521 gggtagcctg aaaacgactt atacccgtag aaatggtaag atttatctta atggtagtaa
    47581 gtgttttatt accagtagcg cctacacccc gtacatcgtg gtgatggcgc gcgacggggc
    47641 ttctccggac aaacctgtct acaccgaatg gtttgttgat atgagcaaac cgggcatcaa
    47701 agtgaccaaa cttgagaagc tcggtctgcg tatggatagc tgctgtgaaa tcacctttga
    47761 cgatgtggaa ctggacgaga aagacatgtt cggtcgggaa ggtaacggct ttaaccgcgt
    47821 caaagaagag ttcgaccatg aacgtttcct ggtagccctg accaactacg gtacggcgat
    47881 gtgcgccttt gaagatgctg cgcgctacgc caaccagcgc gtgcagtttg gcgaggctat
    47941 tggtcgtttc cagttgattc aggaaaaatt cgcccacatg gcgatcaaat taaactccat
    48001 gaaaaacatg ctgtatgaag cagcgtggaa agcagacaac ggcaccatca cctctggcga
    48061 tgcagcgatg tgcaaatact tctgcgccaa tgcggcattt gaagtggtgg atagcgcaat
    48121 gcaggtgctg ggcggtgtcg ggattgcggg caaccaccgc atcagccgct tctggcgtga
    48181 cctgcgtgta gaccgcgttt ccggaggatc tgacgaaatg cagatcctga cgctgggtcg
    48241 tgcggtgctg aagcaatacc gctaagtccg tgcgctgcct gatgcgacgc tgacgcgtct
    48301 tatcaggcct acgggagcac gaatgtaagc cggataaggc gtttacgccg catccggcag
    48361 tcatgcgcta cataaatttt tcaggagcta ataatggatc atctacccat gccgaaattc
    48421 ggaccgctgg ccggattgcg cgttgtcttc tccggtatcg aaatcgccgg accgtttgcc
    48481 ggacaaatgt tcgcagaatg gggcgcggaa gttatttgga tcgagaacgt cgcctgggcc
    48541 gacaccattc gcgttcaacc gaactacccg cagctctccc gccgcaattt gcacgcgctg
    48601 tcgttaaata ttttcaaaga tgaaggccgc gaagcgtttc tgaaattaat ggaaaccacc
    48661 gatatcttca tcgaagccag taaaggtccg gcctttgccc gtcgtggcat taccgatgaa
    48721 gtactgtggc agcacaaccc gaaactggtt atcgctcacc tgtccggttt tggtcagtac
    48781 ggcaccgagg agtacaccaa tcttccggcc tataacacca tcgcccaggc cttcagtggt
    48841 tacctgattc agaacggtga tgttgaccag ccaatgcctg ccttcccgta taccgccgat
    48901 tacttttctg gcctgaccgc caccacggcg gcgctggcag cactgcataa agtgcgtgaa
    48961 accggtaaag gcgaaagtat cgacatcgcc atgtatgaag tgatgctgcg tatgggccag
    49021 tacttcatga tggattactt caacggcggc gaaatgtgcc cgcgcatgag caaaggtaaa
    49081 gatccctact acgccggttg cggtctgtat aaatgtgccg acggctacat cgtgatggaa
    49141 ctggtgggca ttacccaaat tgaagagtgc tttaaagata ttggcctcgc acatctactc
    49201 ggtacgccgg aaatcccgga aggcactcag cttatccacc gtatcgaatg cccttacggc
    49261 ccactggtag aagaaaaact cgatgcctgg ctggcggcac ataccatcgc agaagttaaa
    49321 gaacgctttg ccgaactgaa tatcgcctgt gccaaagtac tgaccgtacc ggaactggaa
    49381 agcaatccac agtatgttgc ccgtgaatca atcactcagt ggcaaacgat ggatggtcgc
    49441 acctgcaaag ggccgaacat catgccgaaa ttcaaaaata accccggaca aatctggcgc
    49501 ggaatgccct cacatggcat ggacacggct gccattttga aaaatatcgg ctacagcgaa
    49561 aacgacattc aggagttggt cagcaaaggt ctggccaaag ttgaggacta aacgcgtggc
    49621 gtcagtcggc ttgcctgact agcgcccgcc caataacaaa cgaacaaaaa tggatagagg
    49681 tgcaatggat atcattggcg gacaacatct acgtcaaatg tgggacgatc tggcggacgt
    49741 ttacggtcat aaaacggcgc tgatttgtga atccagcggc ggagtcgtta accggtatag
    49801 ttatcttgag ttaaatcagg agattaaccg cacggcaaac ctgttttata cgctggggat
    49861 tcgcaaaggc aacaaggttg cactacatct cgacaactgc ccggaattta tcttttgctg
    49921 gttcgggctg gcaaaaattg gcgcgattat ggtgccgatt aacgcccgcc tgttacgcga
    49981 ggaaagcgag tggatcttgc aaaatagcca ggcgtgcctg ctggtgacca gtgcgcaatt
    50041 ttatcctatg tatcaacaga ttcagcagga agatgccact caattgcggc acatttgcct
    50101 gacagatgtg gcacttcccg ctgatgatgg agtgagttca tttactcaac tgaaaaatca
    50161 acaacctgcc accttgtgct atgcaccgcc gctatcgact gacgatacgg cggaaattct
    50221 cttcacctcc ggcaccacct cccgaccgaa aggtgtggtg attacccatt acaacctgcg
    50281 cttcgctgga tattactccg cctggcagtg tgcactgcgt gacgatgacg tctacatgac
    50341 ggtaatgcct gcgtttcata tcgattgcca gtgtactgcg gcgatggcgg cgttttctgc
    50401 cggggccacc tttgtgctgg tcgagaaata cagcgcccgc gccttctggg gacaggcgca
    50461 gaagtaccgc gccaccatta ccgaatgtat tccgatgatg atccgtacgt tgatggtgca
    50521 gccgccttca gcgaacgatc ggcaacaccg cctgcgggaa gtgatgtttt atctcaactt
    50581 gtcggagcag gaaaaagacg cattttgtga acgcttcggc gttcgcttgc tgacgtctta
    50641 tgggatgacg gaaaccattg tgggcattat cggcgatcgc cctagcgata aacgacgctg
    50701 gccgtcgatt ggtcgggcgg ggttttgcta cgaagcggag atccgcgacg atcacaatcg
    50761 cccgctcccg gcaggtgaga tcggtgaaat ctgtattaaa ggcgtaccag ggaaaaccat
    50821 cttcaaagag tattttctca acccgaaagc cactgcgaaa gtgctggaag ccgacggctg
    50881 gctacatacc ggcgataccg gataccgcga cgaagaaggc tttttttatt tcgtcgatcg
    50941 ccgctgcaat atgatcaaac gcggtggcga gaatgtctcc tgcgtggagc tggaaaatat
    51001 catcgccacc catccgaaaa ttcaggacat cgtggttgtg ggtattaaag attcgattcg
    51061 cgatgaagcc atcaaagcat ttgtggtgct gaatgaaggt gaaacattga gcgaagagga
    51121 attttttcgc ttctgcgaac aaaatatggc gaaatttaaa gtgccctctt atctggagat
    51181 cagaaaagat ctgccacgta attgctcggg gaaaataatt agaaagaatc tgaaataaat
    51241 gaaacgtcag ggaactaccc tgccagctaa taatcacgcc ctaaaacaat atgcattttt
    51301 tgcagggatg ttgtcgtccc taaaaaagca aaaatggaga aaaggaatga gtgaatcatt
    51361 acatctgacc cgcaatggat caattctgga aattaccctt gatcgcccaa aagcgaatgc
    51421 tattgatgca aaaaccagct ttgaaatggg cgaagtattt ctaaatttcc gtgacgatcc
    51481 gcaattacgt gtcgccatta ttaccggtgc cggagagaaa ttcttttccg cgggctggga
    51541 tttaaaagcg gcagcagaag gcgaagcacc ggatgctgac tttggcccgg gtggttttgc
    51601 gggattaacc gaaattttca atctcgacaa accagttatc gcagctgtga acggctacgc
    51661 ctttggcggc ggtttcgaac tggcgctggc ggcagatttt attgtttgtg ccgataacgc
    51721 cagcttcgcc ctgccggaag ccaaactggg catcgttcct gacagcggcg gtgtgctgcg
    51781 tctgccgaag atcctgccgc ctaccatcgt caatgaaatg gtgatgaccg gcagacgaat
    51841 gggcgcagaa gaggcgctgc gttgggggat agtcaaccgc gtggttagcc aggcggaact
    51901 gatggataac gcccgcgaac tggctcagca gctggttaac agcgccccgc tggcgattgc
    51961 ggcgctgaaa gagatctacc gcaccaccag cgaaatgccg gtagaagaag cgtatcgcta
    52021 tattcgcagc ggcgtgttga aacactatcc atcggttctg cattcggaag atgccattga
    52081 agggccgctg gcgtttgccg agaagcgcga tccggtgtgg aaaggacgtt aacaaccgtg
    52141 agctattacg cctttgaggg gttaattccg gtggttcacc cgacggcgtt tgtccatccc
    52201 agtgccgtct tgattggcga tgtgattgtg ggagccggtg tctacatcgg cccactcgcc
    52261 tcactgcgtg gtgactacgg gcggttgatc gtgcaagcgg gagccaatat tcaggatggc
    52321 tgcattatgc atggctactg cgacactgac accatcgttg gggaaaacgg ccatatcggg
    52381 catggagcga tcctgcatgg ttgtgtgatt ggtcgcgatg cattggtcgg gatgaacagc
    52441 gtaattatgg atggcgcggt cattggcgaa gagagcattg ttgccgcaat gagctttgtc
    52501 aaagcaggct ttagtggcga gaaacgccag ttgttgatgg gtacgccagc ccgcgctgta
    52561 cgcagtgtta gtgacgacga gttacactgg aaacggttga ataccaaaga gtatcaggat
    52621 cttgtcgggc gctgtcatgc atcgttacat gaaacgcagc cactgaggca aatggaggaa
    52681 aatcgccccc gtttgcaggg gacgacggat gtgacgccta aacggtgatc ttgccggatg
    52741 cgacctggtt gctggataag atgctacaac atcgtatctg gcaagcagga cgcccggtat
    52801 caacgagata aaattaacga cgcatactct ttgacaacat catctgccac ttctgctcgc
    52861 ggttaagttc cgtgagcgga acggcaggaa tacgcggtgt ctttcttacg ccgactaatt
    52921 tttcccgact gttattgcgc aggcgcgcgt aaatttgctc atcgatatcg accactttaa
    52981 ccagtcgctg acactggcat ccccgccctt ccagcttatt agggatcatc ttaacttcgc
    53041 agcttatttc tgtgacttcc gacagaatat aagtcagggt attaaccgcc ttgctgtgtt
    53101 caatatcgaa atgaatagag atctctcttg ctatcaccca ccgattttca gccatcatcc
    53161 attcggcgat taacaagtag agtggttttt caacatatcc ttcacacata cgaactccag
    53221 tttcacaaat taaaagtcta gctatgactc gttttgaagc aattaaacaa ggccatatta
    53281 aaattgtgga tatatcgata gtatgcaact tcaccgtcga taagtgtgag ctaaatcctg
    53341 cttatgtcat caaaaatatc gattccccaa aagatttact taatgggcaa aaaaaaacgg
    53401 tcctcatcag agaaccgtat tagttattcg atcaaattag cggatgaaaa atatccgcca
    53461 cgacacgcta ttatttgatc tgtgcgtgca tttcctgcac cgaaattact ttttcagtcg
    53521 catcggcatt cagcgccatc gcggtagcga aaccaccgtt cagggtggtg tcataatgca
    53581 ctttatattg cagcgcactg cgacggatca cgcgggagtc ttcaatcgca cgacggcctg
    53641 aggtggtgtt gatgatgtag gtatattcgc cattcttgat acggtcctga atgtgcggac
    53701 ggccttcatg caccttgttt accagacgcg gattgatacc cgcttcgccc agcacaatcg
    53761 ccgtgccgtg ggtcgcatcc agctcgaagc cctgtttcag cagttttgcc gccaggtcca
    53821 ccacgcgttc tttatcgcct tcgcgcacgg aaagcagcgc acgaccgtgt ttcttcatgg
    53881 tggagttgct gcccagctgc gctttggcga acgcttcagc gaaggtgcgg cccacgccca
    53941 tgacttcccc ggtagagcgc atttctggcc ctaacagcgg gtcaacgcct gggaatttat
    54001 tgaacggcag caccacttct ttcaccgagt agtacggcgg aataacttct ttggttacgc
    54061 cctgctcagc cagcgatttg ccagccatca cacgcgccgc cacttttgcc agcggtacgc
    54121 cggtggcttt ggagacgaac ggaacagtac gcgccgcacg cgggtttact tcaatcaggt
    54181 aaacttcgtt gtttttcacc gcaaactgca cgttcatcag accgcgcacc tgcaattcga
    54241 aggccagttt ctgcacctgc tggcgcatca catcctgaat ttcctgactt aaggtgtacg
    54301 ctggcagcga acatgcggag tcaccggagt gcacacccgc ctgctcgatg tgctccatga
    54361 tgccgccaat cagcaccatt tcgccgtcgc agatggcatc cacgtcaact tctactgcgt
    54421 catcaaggaa atgatccagc aacactggcg catcgttaga cacgctaacc gccgtctgga
    54481 agtagcgacg caggtcagct tcgtcataga cgatttccat cgcccgaccg ccgagaacgt
    54541 aagacggacg caccaccagc gggtagccaa tctcttttgc cttctcaact gccatttcaa
    54601 tagcagtaac ggtggcgttc gccggttgtt tcagtttcag acggtcaacc gcatgctgga
    54661 agcgttcacg gtcttccgca cggtcgatag catccgggct ggtgccgata accggtacgc
    54721 cagcggcttc cagtgcacgc gccagtttca gcggggtctg accgccgtac tgaacgataa
    54781 cgcctttcgg cttctcaata cgcacgattt ccagcacatc ttccagagtt accggctcga
    54841 agtagaggcg atcggaagtg tcgtagtcgg tagagacggt ttccgggtta cagttaacca
    54901 taatggtttc gtaaccgtct tcacgcagcg ccagcgaggc gtgtacgcag cagtagtcga
    54961 attcgatacc ctgaccgata cggtttggac cgccgccaag caccatgatt ttttcacggt
    55021 cggtagacgg attcgcttcg cactcttctt cataagtgga gtacatgtaa gcggtgtcgg
    55081 tggcaaactc tgccgcacag gtatccacgc gcttgtagac cgggtgcagg tcatattggt
    55141 cacgcagctt acggatttcc gcttcgcgta cgcccgccag ttttgccaag cgcgcatcgg
    55201 caaagccttt gcgtttcagc tggcgcagga attcagcgtt caggccagtg atgcccactt
    55261 ccgccacttt ctcttccaga cgcaccagtt cttcaatctg taccaggaac cagcggtcaa
    55321 tgttagtcag gttgaagacg ccgtccacag acaggcccgc gcggaaagca tcggcgatgt
    55381 accagatacg ctctgcgcct gcgtctttca gttcgcgacg gattttggtt aacgcttccg
    55441 ggtcatccag gctcactttc gggtcgaagc cagttgcgcc cacttccagg ccgcgcagcg
    55501 ctttttgcag ggattcctgc tgcgtgcgac caatcgccat cacttcgcca accgatttca
    55561 tctgagtggt cagacggtcg ttagcaccgg cgaatttttc gaagttgaag cgaggaattt
    55621 tagtaaccac atagtcgatg gacggctcga aggaggccgg agtacgtccg ccagtgatgt
    55681 cgttcatcag ttcgtctagg gtgtaaccca ccgccagttt cgccgccact ttagcaatcg
    55741 ggaaaccggt cgctttcgac gccagcgccg aagaacggga cacgcgtggg ttcatttcga
    55801 taacaatcag gcgaccgttt ttcgggttca ccgcaaactg gacattggaa ccaccggttt
    55861 caacgccgat ttcacgcagc accgccatcg aggcgttacg catgatttga tattctttgt
    55921 cggtcagcgt ttgggctggc gcgacagtga tggagtcacc ggtgtggatg cccatcgcat
    55981 caaagttttc gatagagcag acgatgatgc agttgtcgtt tttatcacgc accacttcca
    56041 tctcgtactc tttccagccg atcagcgact catcaatcag caactctttg gtcggagaga
    56101 gatccagacc gcgggcgcaa atttcttcaa actcttcacg gttataagcg ataccgccgc
    56161 cgctaccgcc catggtaaag gatgggcgaa taatgcacgg gaagcccacg tcagcggcaa
    56221 cagccagcgc ttcttccatc gtgtgtgcga taccggaacg cgcggtttcc agaccaattt
    56281 tcttcatcgc tacgtcgaaa cgacggcggt cttctgcttt atcaatcgca tcggcagtgg
    56341 caccaatcat ggtgacaccg aactcttcca acacgccctg acgttccagc tccagcgcgc
    56401 agttcagcgc cgtctgaccg cccatggttg gcagcaccgc gtccgggcgc tctttttcaa
    56461 taatcttgcg taccacttcc cagtgaatcg gctcgatgta ggtcgcatcg gccatttccg
    56521 ggtcggtcat gatggtcgcc gggttggagt tcaccagaat gacgcggtaa ccctcttcgc
    56581 gcagggcttt acatgcttgc gcgccagagt agtcaaactc acacgcctga ccgataacaa
    56641 tcgggcccgc acccagaatc aggatacttt ttatatctgt acgttttggc atggctcttt
    56701 tactcctgat tacttagcgg ttttacggta ctgctcaatt aacttgataa agtggtcgaa
    56761 caacggcgcg gcgtcgtgtg gaccagggct ggcttcaggg tgaccctgga agctgaacgc
    56821 cggcttatcg atgcgatgaa tgccctgtaa cgtaccgtcg aacagggatt tatgcgtgac
    56881 acgcaggttt gcaggtaatg ttgcttcatc caccgcaaaa ccgtggttct gggcggtgat
    56941 catcaccacg tttttctcca catctttaac cggatggttg ccgccgtggt gaccaaattt
    57001 cattttgaca gtcttcgcac cgctcgccag cgccagcagc tgatgaccga gacagatgcc
    57061 gaataccgga atatcggttt cgaggaactt ctggatggcg gtaatggcgt aatcgcacgg
    57121 ggccgggtcg ccaggaccgt tggagaggaa gatgccgtct ggattcattt tcagcacatc
    57181 ttccgcagaa gtttgcgccg gaacgatggt caggcgacag cctctatcca ccagcatccg
    57241 caggatgttg cgcttggcac caaaatcata agccacgacg tggaacggca gctcgtcttc
    57301 ttttttcgct tctggcaggc cgccggtcaa cgtccagctc ccttgtgtcc agctataggc
    57361 ttctgcggtg gtcacttctt ttgccagatc catgccgttc agacctggga acgcgcgggc
    57421 tttttctaac gccagcgccg catccgggtt atcgcctgcg ataatgcagc cattctgtgc
    57481 gcctttctcg cgcagtaaac gcgtcagctt acgggtatcg atatcggcaa tcgccacgat
    57541 gttatggcgc ttcaggtaag aagagaggtc ttcggtatta cggaagttgc tggcaatcag
    57601 cggcaggtcg cgaatcacca gaccttgtgc atgtacctga gaagattctt catcggcgtc
    57661 attagtgccg acattgccaa tatggggata agtaagagta acgatttgac gagaatagga
    57721 aggatcagtg aggatttctt gataaccggt cattgaagta ttgaaaacga cttccccaac
    57781 cgccgaacct gttgccccta tggcccgacc gtgaaactgg gttccgtctt ccagaaccaa
    57841 tagcgctgac ttaatcaaaa caccctccag agaatactca ctcactttat ttgcatatta
    57901 attcacagtg atgacgtaaa tcaatgcaaa tctgcttacc gacgaatttc tggcaaacgg
    57961 cggcattctg gagatatggg cgctaaaagt caacttaatg gcaggatttt ttatattatt
    58021 ttgtggctca tgcatgtttt acaggcatta agcgacaaaa acatcatcta acctgttctg
    58081 gaaaacgctt gcgcagcaag aagattgcat gaaataaaaa cgagcaggaa aaagtggacc
    58141 aaatggtcaa aatttacatt aaaacaacaa aaatagaaga cgaaacatgt aatttttggc
    58201 agttatagaa aatttaaaag cataacacca aaaataaaag ggctaaaaat taaagccctt
    58261 tataattaat aaattaaatg tgttactttt gcatcataat aaacattttg tggttacaaa
    58321 ttattgagat caagcacatc tcgcatatca aaaagaccac tttccttacc actcaaccac
    58381 aaagccgatc ttaccgcacc gttagcaaat gtcatgcggc tggacgcctt atgggtgatc
    58441 tccagacgct cgccaatatc ggcaaacatc gcggtatgtt cgccaacgat gtcacctgca
    58501 cgcacggtag caaaaccaat ggtacctggc acacgttcac cggtatggcc ttcacgactg
    58561 tagaccgcgc aatctttcag atctttatca agggcgtggg cgatcgcctc tcccattgcc
    58621 agtgcggtgc ctgacggcgc atcaacttta tgtctatgat gtgcttcaat aatttcgata
    58681 tcggtgtagt cacccatcac tttggctgct ttctccagca gcttaagcat gacgttaacg
    58741 ccaacgctaa aattggcggc aaagacaatc gcaatatcgg cggcggcgtc acgaattgcc
    58801 tgtttaccgg cttcgtcaaa ccccgtagtg ccgatcacca tccctttgcc atgctgccga
    58861 caaaaagcga gatggttcag cgtaccttcc ggacgggtaa aatcgataaa cacatcaaaa
    58921 tcatctttta tcgcatcaag actgctttgc acggtaacgc ctgttttccc tgcaccagcc
    58981 agctcaccgg cgtcgctgcc cagtaaagaa gatccttcgc gctccagcgc cgcgcccagc
    59041 tgcacgcctt ctaatgccag tgccgcctga atcaactggc ggcccatacg ccccccggct
    59101 cccgcgatgg caacgcggat gtttgcatca tgcatagcta ttctcttttg ttaatttgca
    59161 tagaccgttt tcagagtaac cagcgcatcg ctacgccgcc agccaaaaac gctgataatt
    59221 agaaattaat gataaacagg gagggatatc agtgaaaggc atgagtcgat gaatgactgc
    59281 atgccgataa catgtgacag gttacggcac cagagccagc acctcagcca cccactgttg
    59341 gaagcctttt acattcagat ccaatgccac ctgtacattg gctggtttgc ccaggcaacc
    59401 gtcgatatca accaccgtcg tgcctgaggt aaattcgcct tgagtttcta ccgcgacaaa
    59461 acacggcttg agggtgaata agtccgggcg caccagccag gcgatggcgc agagatcgtg
    59521 cattcgcaga ccgctttgca tactgccgct acggtagtgg ctaaacaggg cgtggagcat
    59581 ttttccggtg cgattcaact ccggtaaagt agcgagatag tcaggggtta atattgcctg
    59641 attggtgaca tccagaccgc acatgacaat ttcgatgccg ctacggaaga cacaggcagc
    59701 cgcttctgga tcggcagcga tattaaactc ggcatttggt gtacagttgc cgcgaccagc
    59761 agaaccgccc atgatcacca gacgacgaat atacggttta cattccgggc attgtaaaag
    59821 taacagcgca atattggtta acgggccgat ggccaccagg gtaacgggct ctggtgcacg
    59881 catcagggca tcccgaatcg ccagaaacgc cggtatcccg agtggctttc ggttgtgctc
    59941 aacaaagtcg tagccagcca ttcccgattc gccgtgcaca gatgctgcat cacgcggtgc
    60001 gcgtaccagc ggcacagcgg ctccctgggc gagcggaatc tccgcattcc agaaatgcag
    60061 cagttgcagg gcattgcggg tagttttctc aaccgagaca ttacccgcga cggtggtcat
    60121 cagttgcagg tcgagttcgg gggcaaaaat cgcggcggca atggcgacgg catcgtcaat
    60181 gccggggtca gtatcgagga agataggtaa acgcatgttt tcttcataaa aaatgccggt
    60241 aacaagaccg gcattttcgc ataacttagg ctgctaatga cttaatcgac ttcacgaata
    60301 tcgacacgca gctctttcgg cacttcgaaa acaatatttt cttcacggcc ttccagcgga
    60361 atggcttcac caccacccaa ctgctgcaaa cgtgccacca cattctgcac cagaatatcc
    60421 ggagccgatg cgcccgcagt cacgccgacg catttaacct ctttcaccca ctcttcctgg
    60481 atatctttcg catcgtcaat caaaaacgcg tgtttgccca tacgctgggc cagctccgcc
    60541 agacggttgg agttggagga gtttttcgaa ccgaccacca acacaacttc cgcctgttct
    60601 gccagggcgc gtaccgcttc ctgacggtta gtcgtggcgt agcagatgtc atctttgcgc
    60661 ggaccgacaa ttttcgggaa gcgtttacgc agcgcgtcga tcacatcaga cgtgtcatcc
    60721 accgacagcg tggtttgggt cataaaggag agcttctctt cgtttttgac cgtcagtttc
    60781 cacacatcgt ctggcgattc gactagatac attccccctt cggggttgct gtactgcccc
    60841 atcgtccctt ccacttccgg gtgcccggcg tggccgatga gaatagattc ttcgccacgg
    60901 cgactggcgc gggcaacttc catatgcact ttggtcacca gcggacaggt ggcatcaaac
    60961 accgtcaaat cgcggctttt cgcttcgtta cgaaccgcct gagaaacacc gtgtgcggag
    61021 aaaatcagga tcgcgccgtc cggcacttcg ctaatctgct caataaagat agccccacgc
    61081 tcgcgcaagc tatcgaccac gtagcggtta tgcaccactt cgtgacggac atatatcggt
    61141 gcgccgtaaa tggccagcgc gttttcaaca atgctgatag cgcggtctac cccggcacaa
    61201 aaaccacgcg ggttggccaa caggatctgc atgttacgcc tccagtgccg gatcgatttc
    61261 cagcacttca atatcaaaat gaacggtctg cccggccagc ggatggttga aatcaacggt
    61321 aatggagtcg ccgttaattt cgcggatcac accaggcatc tcactgccat ccattgcggt
    61381 aaaaagcatg attgcgccaa tttctggctc gcctgcatcc ataaattcac ggcgggagaa
    61441 gtactgaatc aggtccggtg acggcacgcc aaacgccgca tctggctcca gcgagaaggt
    61501 ggttttgtcg cccactttca gccccagcag gtgttgctcc agcccttcag aaagagaagc
    61561 atcgcccagg cggaacagcg ccggtttacc gttgttgcga gtagactcgg cggtggtgcc
    61621 atcgtcgagt tttagcgtga agtgcaccag gacggcgcta ttgctctgta cagattcaga
    61681 catgcaggtt tgctcttatt tttgaggatt tttgccggat gcggcctacg atttcgtagc
    61741 aatctgtagg ccgcatccgg caggatttat tattgttttt tcgctttgga aggtaaaaac
    61801 ccttccagca cgatcagtgc cgcaccgaca caaatggcag tatcggcaag gttgaaggtg
    61861 gcgaagtgcc agtcgccgac gtagaagtcg atcatatcga caacgaagcc gtgccacagg
    61921 cggtcgaaca ggttgcccag cgcgccgcca ataatcagcg cgtaagcgat attgtttagc
    61981 ttctgcgtgg ccttcgagcg atacatcatc actgccagga gcacgctaat accaatcgca
    62041 ataccggcaa agaaccaacg ctgccagccg ccgctatcgg caaggaaact aaacgccgcg
    62101 ccatagttac gcgcataatg cagattaagc gacgggaaca gcgggaccgt atcccccaga
    62161 gcaaagttct ggaggatcag gtatttgctg cccagatcga taatcagcac gactaccacc
    62221 agccacagcc agcgtagccc tgttgaacag atcgattgac tcatcaggca aacttacgtt
    62281 tttcaccgtc accggcgacg ttgctgacac agcggccgca gatttctgcg tgttccgcca
    62341 ccttgccgac atcctgggtg tagtgccagc agcgtgggca cttctcacct tcggctttac
    62401 tcaacgcgac tttcagccct ttgagtactt cgctctgctg agcatcagca ggtgcgtcgt
    62461 tatagtctgc aacggtagcg ccggaggtca acaggacaaa tcgtaattca tcgcccagcg
    62521 cggtcagttt cgccgccagt tccggttctg catacaaggt tactgccgct tccagcgagc
    62581 cacccacttt cttgtcggca cgcgcttgct caatgacttt gttcacttcg ccacgcactt
    62641 tcaacagctc gtcccagaac gcatcgttca ttgcttcact gtctgccaga ccaaacaggc
    62701 cttcgtacca ctcaccggtg aagacgtatt tttcacgttc gcccggcaga tagccccaca
    62761 cttcatcagc ggtgaaggag aggattggtg ccatccagcg caccagcgct tcagcgatgt
    62821 gatacagcgc agtctggcag ctacgacgcg ccacgctatc cgctttggcg gtgtactgac
    62881 ggtctttgat gatgtcgagg tagaaggaac ccatctcaac ggagcagaag cgcatcagac
    62941 gctgtaccac ttcgtggaaa tcgtatgctt cgtacgcctt gaggatgtct tcctgtgccg
    63001 ctttcgcaca acctacggcc cagcgatcca gtaccaccat ctcttccggt ttcaccatat
    63061 cttttgctgg atcaaaacca ttcaggtttg ccagcaggaa gcgcgcggtg ttacggatac
    63121 gacgatagct atcggcagca cgtttcagga tctcgtcaga aacggccatt tcaccggtgt
    63181 agtcggttga tgccacccac agacgcagaa tatccgcgcc cagtttgttc atcacatcct
    63241 gcggcgaaac ggtgttgccg atggatttag acatcttgcg accctgacca tccacggtaa
    63301 agccgtgggt cagtacctga cgatacggcg ctttgccttt catcgcggtg gagatcatta
    63361 gggaagacat gaaccaacca cggtgctggt cagaaccttc cagatacatg tccgctgcgt
    63421 gaccggcaaa ttccggacgc acgtcaacaa cagaagagtg ggtagatccg gagtcaaacc
    63481 atacgtcgag agtatccggc actttcacat actggtcagc ttcgtcgccg aggatctctt
    63541 tcgcatcgag atcccaccac gcctggatgc cgtcaacttc aacgcgttta gccacttctt
    63601 ccatcagttc gagggtacgc ggatgcagct cttccgtgtc tttgtgcacg aacagtgaca
    63661 tcggtacgcc ccaggtgcgc tgacgggaga tacaccagtc aggacggtta gcaaccatcg
    63721 actcgatacg cgcctggccc cagtccggga gccactgcac gcctttgatc tctttcagtg
    63781 actgcgcacg cagacctttc tgatccatgc tgacgaacca ctgcggtgtc gcgcggaaga
    63841 tgatcggcgt tttgtgacgc cagcagcacg gatagctgtg ctgcattttc tcaacgtgca
    63901 gcagcgcgcc tttttcctgc agcagcgcaa cgacgatgtc gttcgctttg aagacgttca
    63961 cgccatccag cgtcggataa gtgcccggca gataagtgcc gtccgggcca accgggttag
    64021 cggtttccag gccgtatttc tgaccgatca catagtcgtc cgggccgtgg ccaggcgcgg
    64081 tgtgaacggc accggtaccg gcatccaggg taacgtgatc gccgaggatt gccggaacgt
    64141 cgaagcccat aaacggatgg gtaaagcgca gcagctcaag ctctgcacct tttaccgtgc
    64201 cgagaatggt gtaatcggtc acgccgatac gctgcattac gctttcaacc agatctttcg
    64261 ccagaatcac ggcctgaccg tcgatctgca ccagcgcata gtcgaaatca ggtgcaatag
    64321 agattgcgcg gttcgcaggc agagtccacg gcgtagtggt ccagatcacc agcgagattg
    64381 ggccgttaac gttgcttacg ccaaatttcg ctttcagcgc atcctgatcg accgcctgga
    64441 aagcgacgtc aatggacgga gaagttttgt cgtaatactc aacttccgct tctgccagtg
    64501 cagaacggca gtctacgcac cagtgcaccg gcttcgcgcc tttgtgcagg tgaccattgc
    64561 cgatgatttt gcccagcgcg cggatgatgt tggcttcagt tttgaagtcc atagtcagat
    64621 acgggtgcga ccagtcgccc agcacgccca gacggataaa gtctttgcgt tgaccgtcaa
    64681 cctgggtcgc cgcgtattcg cggcacttgg cgcggaactc ggcggcggtg aatttctcac
    64741 ccggcttacc gtattcttgc tctactttca gctcgatcgg cagaccgtgg cagtcccagc
    64801 caggcacata cggcgagtca taaccggaaa gccctttgga cttcacgata atgtctttca
    64861 gaatcttgtt aaccgagtga ccaatatgaa tgctgccatt cgcataagga gggccatcat
    64921 gcagaatgaa ggtttttttg ccttttttag ccgcacggat gatgccgtac agatcatcat
    64981 cagtccaacg cgccagcatt cccggttcgc gcttggcgag atcgccacgc atcgggaacc
    65041 ctgtttccgg caaattcagg gttgatttat agtcactcat cagattctcg gttccgtgtt
    65101 tcggtttgat tacataacag gcttaagccg gttttgttag cccaaaaaat tcgcgggcgg
    65161 ttaattcatc acgcgcaatc tgcgctttca gttcgtccag cgacgcaaat cgctgctcat
    65221 tgcgtatttt tttacgcagc actacttgta tatggcgacc gtaaaggtcc attgcaacat
    65281 ctaacaaatg cacttccagc tgctggcgaa taccggcaac cgttgggcgt gttccgatgt
    65341 ttgccacgcc gggtaacggc ttttcaccga ggcccagcac ttctaccgca taaacccctt
    65401 tcaccgggga aacctgacgg cgtagcggta cattcgccgt cgggaaacct atagtgcgcc
    65461 ctaattcatc accgtggact acacgcccgg agatggcaaa cgggtgcccc agtaaacttt
    65521 ctgccagagc cagattgtca tcagccagcg cctgacgcac ggcggtgctg ctgatacgca
    65581 caccaccttc gcaaaaagtt tgcgtactgg tgatatcgaa gccgtattcc atgccagctt
    65641 tctgtaataa caagaaatcg ccttcacgac cagcgccaaa gcggaaatca tcacctacgg
    65701 caagaaattt tacgcgcaaa tgcttcacca aaagatcgct gatgaaattt tgcgcggtta
    65761 acgccgcaaa acgcctgtcg aaacgcacgc acagcacgta atcaacgcca cactctgcaa
    65821 ggtaacgcag tttttcccgc agccgggtca gtcttgccgg ggctttatcg gtagcaaaca
    65881 gttccagtgg ttgaggttca aaaagcatca ccatcaccgg taagttgcgc ttgcgccctt
    65941 cttcctgcaa gccctgtaac agcgcgcgat gaccgcgatg cacgccgtcg aaattaccaa
    66001 tagtcagcac acacccttct tgcggggcct ggctgagatt atgtatgccg cgtatcagct
    66061 tcatgtctgg ctcaaaacag tgaaaatcgt ccgagtatac cttgtacagc ggtcaaggtt
    66121 aaccggcgat tgagtaccga aatcagaaag aagtggcttt tcattgccat ggagcaaatc
    66181 acgggaagaa actgaccgcc tgctgcaatt tttatcgcgg aaaagctgta ttcacacccc
    66241 gcaagctggt agaatcctgc gccatcacta cgtaacgagt gccggcacat taacggcgct
    66301 tatttgcaca aatccattga caaaagaagg ctaaaagggc atattcctcg gcctttgaat
    66361 tgtccatata gaacacattt gggagttgga ccttggctaa tatcaaatca gctaagaagc
    66421 gcgccattca gtctgaaaag gctcgtaagc acaacgcaag ccgtcgctct atgatgcgta
    66481 ctttcatcaa gaaagtatac gcagctatcg aagctggcga caaagctgct gcacagaaag
    66541 catttaacga aatgcaaccg atcgtggacc gtcaggctgc taaaggtctg atccacaaaa
    66601 acaaagctgc acgtcataag gctaacctga ctgcacagat caacaaactg gcttaatcgc
    66661 caatttgctg aagctttgtg aaaaagcccg cgcaagcggg tttttttatg cctgctgctt
    66721 ttgtgatgcg tgagagaaca cactgccggt tgccagatag tcaaaagaga catgccgttg
    66781 tactatctga actcttcgtg gcttaagaat cgtagaacca tcggctgata cattctgaac
    66841 aagaatatct actcaatctc cagactcccc cacaaagaat atggatattg tgatacacat
    66901 tgaggtatta aacttaagac cactctattt ttgaattaaa ttcctatata ctttaaccat
    66961 tcataaatat ctcctcatta gacct
//
LOCUS       DAJTSF010000007        66730 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000007 DAJTSF010000000
VERSION     DAJTSF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66730
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.007"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO335_000514"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO335_000514"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6001024.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO335_000515"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO335_000515"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6001025.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO335_000516"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO335_000516"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6001026.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO335_000517"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO335_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6001027.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO335_000518"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO335_000518"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6001028.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO335_000519"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO335_000519"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6001029.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OO335_000520"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OO335_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6001030.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO335_000521"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO335_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6001031.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO335_000522"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO335_000522"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6001032.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO335_000523"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO335_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6001033.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO335_000524"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO335_000524"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6001034.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OO335_000525"
     CDS             complement(15665..16702)
                     /locus_tag="OO335_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6001035.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17522
                     /gene="yhhY"
                     /locus_tag="OO335_000526"
                     /pseudo
     CDS             17035..17522
                     /gene="yhhY"
                     /locus_tag="OO335_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(17581..19323)
                     /gene="ggt"
                     /locus_tag="OO335_000527"
     CDS             complement(17581..19323)
                     /gene="ggt"
                     /locus_tag="OO335_000527"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR6001036.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19443..19883
                     /locus_tag="OO335_000528"
     CDS             19443..19883
                     /locus_tag="OO335_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6001037.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19870..20613)
                     /gene="ugpQ"
                     /locus_tag="OO335_000529"
     CDS             complement(19870..20613)
                     /gene="ugpQ"
                     /locus_tag="OO335_000529"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6001038.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20610..21600)
                     /locus_tag="OO335_000530"
                     /pseudo
     CDS             complement(20610..21600)
                     /locus_tag="OO335_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21602..22447)
                     /gene="ugpE"
                     /locus_tag="OO335_000531"
     CDS             complement(21602..22447)
                     /gene="ugpE"
                     /locus_tag="OO335_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6001039.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22444..23331)
                     /gene="ugpA"
                     /locus_tag="OO335_000532"
     CDS             complement(22444..23331)
                     /gene="ugpA"
                     /locus_tag="OO335_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR6001040.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23429..24745)
                     /gene="ugpB"
                     /locus_tag="OO335_000533"
     CDS             complement(23429..24745)
                     /gene="ugpB"
                     /locus_tag="OO335_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR6001041.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25142..25855)
                     /gene="livF"
                     /locus_tag="OO335_000534"
     CDS             complement(25142..25855)
                     /gene="livF"
                     /locus_tag="OO335_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6001042.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25857..26624)
                     /gene="livG"
                     /locus_tag="OO335_000535"
     CDS             complement(25857..26624)
                     /gene="livG"
                     /locus_tag="OO335_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6001043.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26621..27898)
                     /gene="livM"
                     /locus_tag="OO335_000536"
     CDS             complement(26621..27898)
                     /gene="livM"
                     /locus_tag="OO335_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6001044.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27895..28821)
                     /gene="livH"
                     /locus_tag="OO335_000537"
     CDS             complement(27895..28821)
                     /gene="livH"
                     /locus_tag="OO335_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6001045.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28869..29978)
                     /gene="livK"
                     /locus_tag="OO335_000538"
     CDS             complement(28869..29978)
                     /gene="livK"
                     /locus_tag="OO335_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6001046.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30402..30785
                     /gene="panM"
                     /locus_tag="OO335_000539"
     CDS             30402..30785
                     /gene="panM"
                     /locus_tag="OO335_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6001047.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30984..32087)
                     /gene="livJ"
                     /locus_tag="OO335_000540"
     CDS             complement(30984..32087)
                     /gene="livJ"
                     /locus_tag="OO335_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6001048.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32358..33212)
                     /gene="rpoH"
                     /locus_tag="OO335_000541"
     CDS             complement(32358..33212)
                     /gene="rpoH"
                     /locus_tag="OO335_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6001049.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33457..34515)
                     /gene="ftsX"
                     /locus_tag="OO335_000542"
     CDS             complement(33457..34515)
                     /gene="ftsX"
                     /locus_tag="OO335_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6001050.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34508..35176)
                     /gene="ftsE"
                     /locus_tag="OO335_000543"
     CDS             complement(34508..35176)
                     /gene="ftsE"
                     /locus_tag="OO335_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6001051.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35179..36675)
                     /gene="ftsY"
                     /locus_tag="OO335_000544"
     CDS             complement(35179..36675)
                     /gene="ftsY"
                     /locus_tag="OO335_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6001052.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36825..37421
                     /gene="rsmD"
                     /locus_tag="OO335_000545"
     CDS             36825..37421
                     /gene="rsmD"
                     /locus_tag="OO335_000545"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6001053.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37411..37680
                     /locus_tag="OO335_000546"
     CDS             37411..37680
                     /locus_tag="OO335_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6001054.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37683..38042)
                     /locus_tag="OO335_000547"
     CDS             complement(37683..38042)
                     /locus_tag="OO335_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6001055.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38183..38809
                     /locus_tag="OO335_000548"
     CDS             38183..38809
                     /locus_tag="OO335_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6001056.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38883..41081
                     /gene="zntA"
                     /locus_tag="OO335_000549"
     CDS             38883..41081
                     /gene="zntA"
                     /locus_tag="OO335_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6001057.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41183..41428)
                     /gene="tusA"
                     /locus_tag="OO335_000550"
     CDS             complement(41183..41428)
                     /gene="tusA"
                     /locus_tag="OO335_000550"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6001058.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41649..42314
                     /locus_tag="OO335_000551"
     CDS             41649..42314
                     /locus_tag="OO335_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6001059.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42387..42944
                     /gene="dcrB"
                     /locus_tag="OO335_000552"
     CDS             42387..42944
                     /gene="dcrB"
                     /locus_tag="OO335_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6001060.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42948..44165)
                     /locus_tag="OO335_000553"
     CDS             complement(42948..44165)
                     /locus_tag="OO335_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6001061.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44299..45348
                     /locus_tag="OO335_000554"
     CDS             44299..45348
                     /locus_tag="OO335_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6001062.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45403..45990
                     /gene="acpT"
                     /locus_tag="OO335_000555"
     CDS             45403..45990
                     /gene="acpT"
                     /locus_tag="OO335_000555"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6001063.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46101..47675
                     /gene="nikA"
                     /locus_tag="OO335_000556"
     CDS             46101..47675
                     /gene="nikA"
                     /locus_tag="OO335_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6001064.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47675..48619
                     /gene="nikB"
                     /locus_tag="OO335_000557"
     CDS             47675..48619
                     /gene="nikB"
                     /locus_tag="OO335_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6001065.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48616..49449
                     /gene="nikC"
                     /locus_tag="OO335_000558"
     CDS             48616..49449
                     /gene="nikC"
                     /locus_tag="OO335_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6001066.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49449..50213
                     /gene="nikD"
                     /locus_tag="OO335_000559"
     CDS             49449..50213
                     /gene="nikD"
                     /locus_tag="OO335_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6001067.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50210..51016
                     /gene="nikE"
                     /locus_tag="OO335_000560"
     CDS             50210..51016
                     /gene="nikE"
                     /locus_tag="OO335_000560"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6001068.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51022..51423
                     /gene="nikR"
                     /locus_tag="OO335_000561"
     CDS             51022..51423
                     /gene="nikR"
                     /locus_tag="OO335_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6001069.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51543..51902)
                     /locus_tag="OO335_000562"
     CDS             complement(51543..51902)
                     /locus_tag="OO335_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6001070.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51899..52174)
                     /locus_tag="OO335_000563"
     CDS             complement(51899..52174)
                     /locus_tag="OO335_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR6001071.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52233..53357)
                     /locus_tag="OO335_000564"
     CDS             complement(52233..53357)
                     /locus_tag="OO335_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6001072.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53357..56092)
                     /gene="rbbA"
                     /locus_tag="OO335_000565"
     CDS             complement(53357..56092)
                     /gene="rbbA"
                     /locus_tag="OO335_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6001073.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56089..57156)
                     /locus_tag="OO335_000566"
     CDS             complement(56089..57156)
                     /locus_tag="OO335_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6001074.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57522..59144)
                     /locus_tag="OO335_000567"
     CDS             complement(57522..59144)
                     /locus_tag="OO335_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6001075.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59406..60995)
                     /locus_tag="OO335_000568"
     CDS             complement(59406..60995)
                     /locus_tag="OO335_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6001076.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61380..62411
                     /locus_tag="OO335_000569"
                     /pseudo
     CDS             61380..62411
                     /locus_tag="OO335_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62498..62974)
                     /locus_tag="OO335_000570"
     CDS             complement(62498..62974)
                     /locus_tag="OO335_000570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001077.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62967..63731)
                     /locus_tag="OO335_000571"
     CDS             complement(62967..63731)
                     /locus_tag="OO335_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6001078.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63977..64738
                     /locus_tag="OO335_000572"
                     /pseudo
     CDS             63977..64738
                     /locus_tag="OO335_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(64845..65345)
                     /locus_tag="OO335_000573"
     CDS             complement(64845..65345)
                     /locus_tag="OO335_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001079.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65496..66644)
                     /locus_tag="OO335_000574"
     CDS             complement(65496..66644)
                     /locus_tag="OO335_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6001080.1"
                     /translation="MSITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQP
                     LGNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDR
                     KLSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAH
                     QSQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDG
                     TLALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTS
                     QLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDG
                     IAYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16217 a        17907 c        16362 g        16244 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactgtcgc ctgtattgat ggagacgtcg
    17221 tgggccatct caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg
    17281 gtatctgtgt cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga
    17341 tgattgaaat gtgcgacaac tggttacggg tagatcgcat tgaactaacc gtgtttgtcg
    17401 ataacgcgcc agcaattaag gtctataaaa aattcggctt tgagattgaa gggaccggta
    17461 agaagtacgc attgcgtaat ggtgaatatg tcgatgcgta ttatatggcg cgggtgaaat
    17521 agagaatttg caatctaagt ttgtcattta agccctcacc taccgggagg gcttaagata
    17581 ttaatacccc gccgttaaat catccaccga gcgcgggtcg gatgcgccgt acaactcacc
    17641 gtccggccca accataatgc tttgtgtgct gcccatcgcc tctttcagcg ccactttctg
    17701 accttttgct tccagcagct tgagcgtatc cgggctaaac cctttttcga cacgcagctc
    17761 gtccggcaac cactgatggt ggaaacgcgg cgcattggtc gcttcggcga cgttcatgcc
    17821 ataatcgatg ctattcacca ccatttgcag caccgtggtg ataatccggc taccgcctgg
    17881 gctaccggta accagccagg ttttaccgtc tttcaccaca atggtcggcg acatcgacga
    17941 cagcgggcgt ttgttcggcc cgacggcgtt ggcatcaccg cccaccagcc cgtaaacgtt
    18001 cggtacgccc ggtttggcgg agaaatcatc catctggtta ttaagcagaa taccgctctc
    18061 acccgcgaca atacccgtac cgaaggtggt gttcagcgta taggtcaccg ccaccgcgtt
    18121 accgtcttta tccactacag agtaatgggt agtttgatta ctctcataag gcgcaagctt
    18181 gccagggcga atctcactgg acggcttcgc ttttttgata tcaatttgat cggcaatgga
    18241 tttggcatag gctttattgg tcagtgcctg ccacgggact ttgacaaaat ccgggtcgcc
    18301 aagatattcc gagcggtcgg cgtaggcgta tttctccgct tctgccatga tttgcatcgc
    18361 atcggcgctg ccaaagccgt atttcttcat atcgaagttt tccagaatat tgaggatttg
    18421 tacgatatgg atcccgccgg aggatggcgg tggcatggag taaacctgat acccgcgata
    18481 atcgccgctt atcggagtgc gttcgaccgc tttataggcc gctaaatctt ctttagtgat
    18541 caagccaccg tttttctgca tctcctgggc gatctgttcc gcaatcgtgc ctttatagaa
    18601 ttcgtccggg ccgttttcag caatcatctc caggctcttt gccaggttcg cctgcaccag
    18661 cgtgtcgccc tttttcagcg gctcgccctc tttccagaag atagctttac tgttttcgtg
    18721 attcggcaac acttcgctac cgtaggtttt gagatcgtca gccagcgcgt cgttaacgat
    18781 aaaaccatcg cgtgccagtt taaacgcggg ctgcacgact ttgttcagcg gcatggtgcc
    18841 gtatttatcc agcgccagcg agaaacctgc taccgtaccc ggtgtgccgg aagccagatg
    18901 cgaagtgagt gattttttgc tgtccgggtt gccctgatca tcgaggaaca tatcgcgggt
    18961 cgctttggcg ggtgccattt cgcggaaatc gatagccgtg gtattgccat ttttcgagcg
    19021 gattaacata aaaccaccac cgcccagatt ccctgcctgc ggatgcgtta ccgccagcgc
    19081 gtagcccacc gccacggcgg catcaacggc attcccgccc tccttgagga tatccacccc
    19141 cacctgagtg gcagtggcgt ccacagacgc taccattccc tgtttcgcgc gtaccggatg
    19201 gaagacatct tcctccacac catacgagac gggcggcgca ggaggcgcgg cggcggcgct
    19261 aaaacaactt cctgagagca gagcagcaat ggccacccgg cgtaaaaacg tcggttttat
    19321 catcgttatt ctccagagat taaggggcaa ccccagctaa gcctggtata taactctgaa
    19381 ttaatcatcg ttttgtcggg aagcgagtaa acttaaagga tatcttcaga ggaggatacg
    19441 cgatgaaacg acttctgatt cttacggcac tcctgccgtt tgtcggcttt gcacagccca
    19501 ttaatactct gaacaaccct aaccagccgg ggtatcagat acccagccag cagcggatgc
    19561 aaacccagat gcagactcag caaatccagc aaaaagggat gctgaatcag caactgaaaa
    19621 cgcaaactca gttgcaacag cagcatttag aaaaccagat aaacaataat tctcagcggg
    19681 tgttgcagtc acagccgggg gagcgaaatc ccgcccggca gcaaatgctg cccaacacca
    19741 acggcgggat gttaaacagc aaccgtaatc cggatagttc gttgaatcag cagcatatgc
    19801 tgccggagag gagaaacagc gacatgctga atcagcccag cacgccgcag cctgacattc
    19861 cgttgaaaac tattgggccg taaagttcgg accaatcacg tcaatcgcat cggtacagat
    19921 gcaatccacg ccccagcgca gcaactctgc cgcgtgctgg ggtttattga cggtataaac
    19981 cagaatccgt aatccggcgt ctttcaactg catcactcgc gctttatcga gtaacttatg
    20041 attgagatga atagagacgc agcccagccg cgcggtcagt tcgcgccagt cgtcgcgcca
    20101 ctcatcaagc aataaaccac gcggcagttc cggtgccgcc tgttgtgcag cttctaaagc
    20161 atcaatctca aacgatgaca gcagcggcgg cgtcataccg gcccacagtt ggcgtgccgc
    20221 cagcgccacc attttgcccg ttaatggtcc agtgccagtg gtgggtttga tttcgatatt
    20281 cgccatcatc ccgtgttcgc gacagcgttc cgccacctgc gaaagcaacg gcagcggctc
    20341 acctttaaac gctttgctgt accaactgcc cgcatccacg cgcagtaaat cctgccagtt
    20401 cagttcaccc gcgacgcccc agccgttgct ggtgcgttcg agattgtcgt catggagcag
    20461 gaagatctcg ccatctttcg ataacttcgc gtcaaattcg atcatcttat gaccgtattt
    20521 tgccccgacg tcgattgccg ccagggtgtt ttccggggcc agcttaccgc cgccacgatg
    20581 agcgacgatg cggggataag gccagttact catactcgtt gtcctgtttc accatcaaaa
    20641 agatgcagct gattttccgc cagatgcagc cacagcgtgc tgcctgccgt cgggcgctcc
    20701 tgatgcgcca gtcgcaccac cagcttctgc ttgcgagctt agcgcaatat gttccgggcg
    20761 aatgccgagg gtcattttac gcccggcata ctgacggtag ccaccgttta gcggtagcac
    20821 gataccgccg tccagttcga aatgcgtgcc ttcattattc atgcggcctg tcagcaggtt
    20881 catcgccggg ctgccgataa aactcgccac aaacaggctg gcgggctttt cgtagacttc
    20941 caccggcgtg ccaatctgtt cggcaacgcc gccgttcatc accattactc gctgggcgag
    21001 cgtcatcgcc tcaacctgat cgtgagtaac gtagagtgaa gtcgttttca ggcgacggtg
    21061 cagctgttgc agttcaagac gcatctgtac gcgcagcttg gcatcgaggt tagagagtgg
    21121 ctcatcaaac aagaacaccg ccggatcgcg cacaatcgcg cgtcccatcg ccacacgctg
    21181 gcgctgaccg ccggaaagct cgcgcgggcg acgtttgagc agaccgtcca gctccagaat
    21241 gcgcgccgct tctttaacgc gctcggctat ttgctgcttg cccatgccgc gaattttcag
    21301 cccccacgcc atgttttctt cgacgctcat atgcggataa agcgcgtaat tctggaacac
    21361 catcgcaatc ccgcgatctt tcggctccat ttcggtcacg cgctggtcgt tgatccagat
    21421 atcgccttcg gtcaccctct ccagcccggc aaccatacgc agcagcgtcg atttcccgca
    21481 gccagacggc ccgaccatca cgataaattc gccatccgcc acatcaaggg tcagcggttt
    21541 aatgacctag gttttgccat cccagctttt ggttactgcc tgtaatttca gtcctgccat
    21601 cttatttctc actatcgacc aggccgcgca cgaaggcacg ctgcatcact aaaacaatca
    21661 ctactggtgg gatcagggtt aacaacatcg ccgccatcac tgagttccat tccgtggtgc
    21721 cttcgccagt ggcgatcatc cctttgatcc ctgccacggt ggtgccgaga tccacgtcgg
    21781 taataatcaa caacggccac aaatactggt tccagccgta aataaaggtg atcacaaaca
    21841 gcgccgccag attagttttg gagagcggaa aaacgatgtc gcaaaagaag cgcattggtg
    21901 acgcaccgtc gatccgcgcc gcttccacca gttcatccgg cagcgtcata aagaactggc
    21961 gaaacagaaa cgtggcggtc gccgaggcca tcagcggcag cgttaaaccg gcgtagctgt
    22021 cgagcatctt caggttggcg atgacttcca ccgtcgggaa gatacgtact tcaaccggca
    22081 gcatcagggt gataaaaatc atccagaaga agaggttacg tagcggaaaa cgaaaccaga
    22141 caatggcaaa tgccgagagc atcgagacag taattttgcc gagcgtaatg ctgaacgcca
    22201 tcacaaagct gttaagcaac atccgccaga acggcgcgct atttgtgcct accccgttca
    22261 cccagatgtt gtggatgttt tccagcagat gcgtgccggg gatgagcgtc atcggcgcgg
    22321 cgtagacggc ctgtttatcc agcgtcgccg cgacaaacgc cacgtacagc gggaagagga
    22381 tcaccgcgat cccgaggatc agcatggtat ggctgaatat cgtcagccac ggacggttct
    22441 caatcattgg taacgcacct tgctttcaac atagcggaac tgcaccaccg tcagcacgat
    22501 gacgaggaac atcagcacca tcgactgtgc ggcagacgaa gccagatcca gtccggtaaa
    22561 accttcgcgg tagatcttat aaatcagcgt cgtggtggcc tgaaccggcc cgccggacgt
    22621 ggcggcgtcg atcaccggga aggtgtcgaa gaaggcatac accaggttca ctaccagcag
    22681 gaagaaactc accggggcga taagcggcag cgcaatctta aagaagcggc gaatcggccc
    22741 tgcaccgtcg atggctgcgg cttcgatcaa cgaacgggga atggattgca gcgcggcata
    22801 gaagaacaga aagttgtagc tgatttgctt ccacaccgag gcaaacacca ccagaaacat
    22861 cgcctgaccg ctattttgcg cgtggttcca gtcgtagccg aactcagcga gaaaatgggt
    22921 gatcagcccg cgaccggggt taaacaggaa gatccacaat acggcggcaa cggcgggagc
    22981 cacggcatac ggcagcaaca ttaaggtttg gtagaaacga ctgccgcgca cgatgtactc
    23041 caccagcgcc gcaaagaaca gcgataccag caaaccgctg acggtgacaa aggtgctgaa
    23101 ttttatcgtc gtccagaagg agtcgagata atagctgtca tgaaacagcg tgacgaagtt
    23161 atccagaccg acaaactggc tggagaaacc aaacggatcg acgctttgta gcgagtacca
    23221 caacgcttcg cccgcaggcc agataaaaaa gataacggtg atgatgagct gcggcgcgac
    23281 cagcagataa ggcagccagc gcgagcggaa caccggacgg gatgatgaca ttacggttaa
    23341 ttcctgaact gtgccggatg cgcttcgctt atccggccta cacgcgatgc caggtcagat
    23401 aagcgaaacg catccggcat tacactgatt aagacttcgt cgatttctca aagcggcgca
    23461 gtaactggtt cccacgctca actgcggtat ccagcgcctg ctgtggcgtc ttcttaccgg
    23521 tccacacgct ctcaagctct tcatccacaa tcacgcggat ctgcggcatg ttgcccagac
    23581 gaagcccttt ggtgaacggc aacggcggct tgttcagcat ctgacgcgtc gcggtatccg
    23641 cacctgggtt tttctcgtaa aagccctgct cacgggtcag gtcatacgcc gctttggtaa
    23701 ttggcagata accggttttc tgatgccact cagcagcgtt ttctggcttc gcgaggaagt
    23761 cgagaaactt cgccacgccg gtatacgttt ctttatcttt gccctgcatc acccacaggc
    23821 tggctccgcc gataatggcg ttttgtggcg catctttcgc atcggcgtcg taaggcatca
    23881 tacctacgcc gtagttaaat ttggcgtact cgcgaatgtt ggcaagagaa ccggaagagg
    23941 cggtggtcat cgcgcaatca ccgttataga acttctcggt ggattcatcc ttacgaccga
    24001 cgtagctgaa gtcgcccttc ttgttcatct cctcgagcat ggcgatgtgt ttcacctgct
    24061 ccggcttgtt gaactccagc accgcgtccg tgccgtcaaa gccgttgttt ttgctggcaa
    24121 acggcagacc gttccaggcg ctaaagtttt ccagttggat ccagccctgc cagccgctgg
    24181 cgtagccgca cttaataccg gaggctttca gttttgcgga atagtccgcc agatcctgcc
    24241 aggttttcgg cggctgttcc gggtctaacc ccgctttctt gaaggcgtct ttgttgtaat
    24301 agagaacgga agtcgagctg ttgaacggct gggagagtaa gtggccggtt ttgctgtcgg
    24361 agtagtaacc ggaaaccgtc ggcacaaact gcgactcatc gaactgaatc cctgcctctt
    24421 taaacacgtc atacaccggt ttaatggctt tcgacgccat catggtggcg gtgccaactt
    24481 cataaacctg caaaatagcc ggagcgttgc cggtacgaaa tacggcaatc cccgcgctta
    24541 aattctgttc gtagttgcct ttataggtcg gtacaatttt gtaatccgga ttttcggcgt
    24601 taaaacgttg ggccagagaa tccacctctt tacccagttc cccttccata gaatgccaga
    24661 acggaatggt cgtcactgcc tgtgcattcc ccattaacgc cagtccgagc gccagtgctg
    24721 aagctgtata atgtaacggt ttcatcgttt atctctcttg ttgtaccgaa tgcgcgaatt
    24781 cacgcgtttt atgctcgcgg ggtaacatga catgctcgaa ttacagaaaa ataacttttt
    24841 tgttacattt gtaagatagt aaggtgtcag aaagatgaca aggcggtgac ggcgtgggtg
    24901 agggaaaatg ggagatgggg cacggataag cggaaaaata gaaggtctga atcaaactct
    24961 acagattact catcgtttca tgccggatgc ggcgtaaacg ccttatccgg cctacaagat
    25021 cgtgcaaatt caatatattg caactcaccc agtaggcctg ataagcgcag cgcatcaggc
    25081 aattttgcat ttgtcacctg tctcaaagga gtcttttgac tccttatcaa tcaacgtgtt
    25141 attacccgcc taaatacgca cttctcactg cttcgttcgc cagcagcgcg tcgccggtat
    25201 cggagagcac cacatggccg ttttccagca cgtagccgcg atccgccagc tttagcgcct
    25261 ggttggcgtt ctgctcgacg aggaagatag tcatcccctg ctcgcgcagc tgctcgatgg
    25321 tgtcgaaaat ttgctggatg ataatcggcg caagaccgag cgatggctca tcaagtaaca
    25381 acaaacgcgg attgctcatc agcgcgcgac caatcgccag catctgctgt tcaccgccgg
    25441 acatggtgcc cgcccgctga acacggcgct catgcagacg tggaaacagt tcatacaccc
    25501 actttatgcg ctcctgaaac tggtcacgtt cggcaaagaa gccgcccatc gccaggttct
    25561 cttccaccgt catccgcgag aagacgcgac gcccttccgg gacaatcgct accgcttcgc
    25621 gcatgatttt cgccgtctgc cagtcggtaa tgtctttatc atcaaacaca attcgcccgc
    25681 tggtggcgcg cggatcgccg cataacgtgc cgagcaaggt ggttttcccc gccccgttcg
    25741 cgccaatgag agtgacaatc tcgccctgat tgatatgcag gctcacctca tgcagcgcct
    25801 ggattttgcc gtagtgggcg ctgactttgt caaaggacaa catgactttt tccatcttat
    25861 gcctcaccta aataggcacg gatcacgtcc gggttattac ggatttgctc cggcgtaccg
    25921 tttgccagtg gcgtcccctg attgaccaca taaattcggt ctgaaatccc catcaccagc
    25981 ttcatatcgt gttcaatcaa caaaatagtg gtgttgtgat gattgcgcag ttcggcaatc
    26041 agctcatcca gctctttggt ttcttttggg ttaagacctg ccgccggttc gtcgagcatt
    26101 aaaatctccg gctgcgtcac catgcagcgg gcaatctcca gacggcgttg gtcaccatag
    26161 gccaggttac tcgcctgacg gttggcgtgt tccagcaaac caatgcgctc aagccaggtg
    26221 gcggcgcggt cgagcgcttc gctctgggcg cgacggaaag aaggcgtttt caacaggcca
    26281 gagaacagcc cggttttcag ttgctgatgc tgcgccacca gcaggttttc aattaccgtc
    26341 atttcacgga acagacgcac atgctggaag gtgcgcacca cgcccatgcg agcaatttgc
    26401 tgccccggta aaccttcaag atgctgatcg cgcagtaaaa tggtgccgcc ggtgggtttg
    26461 tagaaaccgg tcaggcagtt aaaaaccgtg gtttttccgg caccgttagg gccgattaac
    26521 gagacgattt cctgcgggta cagttcaaga ttgacgttgt tcaccgccag caggccgccg
    26581 aagcgcatca tcagaccgtt aacagataat aatggctgac tcatgcctgc tctcctttcg
    26641 ctgcgccgtt tttcagcttc agttgcgggc gcgtcatggg cagcaaaccc tgcggacgcc
    26701 agatcatcat cagcaccatc aaaccaccga gcattaacat gctgtattcg ttgaaatcac
    26761 gcatcaattc gcgcgacacc accagcagaa cagccgccag aatcactgca aattgcgagc
    26821 ccataccgcc gagcaccact atcgccagca caaaggccga ttcagcaaag gtgaaggatt
    26881 ccgggctgac aaagccctga cgcgccgcaa acagcgttcc ggcaaaaccg gcaaatgcgg
    26941 cgctgatggt aaaagcagtc agcttgatac ggcgcgggct taagcccagc gaacgacagg
    27001 cgatctcgtc ttcacgcaac gcttcccacg cgcggcccag cggcatccgc agcaggcggt
    27061 taatgacaaa caggcttaac accaccagca gcaacgccac caggtagagg aagataacgc
    27121 gatcggacgg atcgtatttc aggccaaaga aattactgaa cgtgtcccag ccgccttcac
    27181 gagcggtacg gctgaactcg agtccgaaga gtgtcggttt cgggatctga ctgattccgt
    27241 tcgggccgcc ggtgatttca gtgttattga gcagcaatat gcgcacaatt tcgccgaaac
    27301 caagggtaac gatcgccaga tagtcaccgc gcagacgcag caccggaaaa ccgagcagga
    27361 aacccgccgc cgctgccatc agtccggcaa tcggcaaaca ggtccagaac cccagaccgt
    27421 aatagtgatt gagcagcgca aaagtgtaag cgccgatggc gtaaaaaccg ccgtacccca
    27481 gcaccagcag accagaaaga ccaacaacca cattcagccc gagaccgagg ataatgtaga
    27541 tcatggtcag agtggcgata tccaccgtcc cacgtgacac cataaacggc cacgccacca
    27601 caagcaccag cagcgccacg aggaacagct tttgcttcac cgtggaccca tcaatcgcgg
    27661 gcagaataaa cttcggcccg gaaacgcttt tcaacccttt ctggaaagcc ggtcgcaaaa
    27721 gctggaagaa aaagaccacc gccgtgccga taaacatcca ctgccaacgg atgtctgaag
    27781 ccgtgtcgac caccagtttg gtgccatcca gctccagttg tacgcccata aagacgcccg
    27841 ccaggacaaa gaacatcgcg gcagagagca gtgccattgc aatatgcatc ggtttcatac
    27901 tttctctacc tccgggcgac ccagaatacc ggtcggcatc accagcagca ccagaatcag
    27961 cagggcgaat gagaccacat ctttatattc cgtactcaga taggcagaag agagcgcctc
    28021 cgcaatcccc aggatcagcc cgccaatcat cgcccctgga atgctgccaa tcccaccgag
    28081 caccgcagcg gtaaaggctt tcatcccggc cataaagccg atataggggt taatgacgca
    28141 gtagaactga ccgagcagta cgccagccac tgccgccatc gccgcgccaa taacaaaggt
    28201 caaagcaatc acccggtcgg tgttgatgcc aagcaggctc gccattttca gatcttccgc
    28261 gcaggcacga caggcgcgcc ccatacggga atagcgaatg aaaatcgtca gcgccagcat
    28321 ggcgaggaag gtgacaatcc agatcaccgc ctgcatggtg gtaatagagg cagagaagtt
    28381 ttcgctatgg cccaccaccc actgaccgtt aaacaggctc ggcagcgcca cgtcgcgcga
    28441 accttcggtc aggctgacgt agttttgcag gaagatggac ataccgattg cggaaataag
    28501 cgcaatcagg cgcttagagt tacgcaccgg gcggtaagct acccgttcga tactccagcc
    28561 gtaggcgctg gcgatgacga ttgcgccgac gaatcccgcc gccaccagca gccagccggt
    28621 atcaatgccc atcatcatca gcgcggcgat gatcataaat gagacgtagc tgccaatcat
    28681 ataaacctcg ccgtgggcga agttgatcat gccgataatg ccgtaaacca tggtgtagcc
    28741 gatggctatc agcgcgtagg tactgcccag cgtgacgccg ttaaacatct gctgcaagaa
    28801 atacaaaaac tgctcagaca taaggtaacc tttctaaacc cgcccgcatt ttacgggcgg
    28861 tgggatgatc acttggctgc cgtggatgaa ccgtcggcgt gccactggaa gacaccaaaa
    28921 tcaaatccct taagatcgcc tttttcatcc cagttcagcg gcccaatcac ggtgtttgca
    28981 ccgttagctt ttaaatcttt caccagcgcc agcggctcat cgctgccggt acgctcaagg
    29041 gcagttgcca gagattgcac tgccgcgtaa gtgatccaga cataaggccc ggacggatct
    29101 ttcttgtctg ctttcagcgc atcaacgatg ccctggtttg ccggatcctg gtcatagcgt
    29161 tttggcatag tgaccaacat gccttcggcg gcatcaccgg caatgttcga cagcgatgcg
    29221 ttaccgacac cttccggccc cataaactgg gttttcaggc caacggaacg ggcttggcgc
    29281 agcatctgcc ccatttccgg gtagtaaccg ccgtagtaaa cgaagtcgat attttctttt
    29341 ttcaggcggg cgatcagcgc ggagaaatct ttctccccgg cggtaatacc gtcgaagaag
    29401 acgacgttgg cgttagccgc tttcagcccg tcctgcaccg aacgcgccag cccttcgcca
    29461 tactgttgtt tgtcgtgaat gatggcgatg cgctggggct tcaccgtctc aagaatgtat
    29521 tttgccgccg taggcccctg ggaagagtcc agcccggcag tacgcataat gtgttgataa
    29581 ccgcgttggg tcagctccgg gttggtcgct cccggcgaga tcatcagaat accttcgtct
    29641 tcgtagatat ccgacgctgg ctgggtagag gaagaacaca gatgaccaat aacgtattta
    29701 atgccgtcgt taacgatttt gttggcgacc gcaacggctt gtttcgggtc gcaggcgtcg
    29761 tcatattcca cgccaaccag cttgtcgccc ttaattcccc ctttgacatt aatgtcttta
    29821 atcgcctgac gcgcgccgtt aaattccata tcgccccact gggcaatcgg gccggacatc
    29881 gcgccgacaa cggcgacttt aatatcgtca gccatagcgg tgtgtgaaat tgccagtgca
    29941 atcatccctg cgatgatagt tttcgcattc cgtttcatag tcaaaaatcc ccattcgtga
    30001 tgttgtgttg ctttgttttt atgtgttaac aaatcagact gttctttttt tatactgcac
    30061 tgtttttgcc tgtctgatta aggggttagc gcagtatttt gtgatgatag cgattaaaat
    30121 ccctattttt cagtcgatta agaacagata atattctgaa tttattgata gataaacaga
    30181 aaaaagtgcc tttgtcagca taaaataacg gcacaaaggg cggaataatt cactatcatt
    30241 caggggatta tgctggacat ttttcattct ctaatgtttt aactttgtaa ttattgctgt
    30301 taaaaaatta atcacctgcc aaaagaaata aaaaagagaa agcctccgat taaattattt
    30361 cgctacactg gttccacttt tgtgatttac acgggttacc catgaagctg accatcattc
    30421 gattagaaaa ctttagcgac caggaccgga ttgacctgca aaagatctgg ccggagtatt
    30481 ccccttcctc tttacaggtt gacgataacc accgtatcta cgccgcgcgt tttaacgagc
    30541 gtctgcttgc ggccgtgcgg gtaaccttaa gcggcaccga gggagcactg gattctctac
    30601 gcgtgcggga agtcacccgc cgtcgcggtg tggggcaata tctgctggaa gaggttttgc
    30661 gtaacaatcc tggcgtttca tgctggtgga tggcggatgc aggcgtggaa gatcgcggtg
    30721 tgatgacggc gtttatgcag gcactgggat ttacggcaca gcagggcggc tgggagaagc
    30781 gttaatcgtc aagttgattt caaaagtgat gaccaacgag aaattgcctg atgcgctacg
    30841 cttatcaggc ctataatgtg tgctgcaatt tactgatttc tttggttctt gtaggccgga
    30901 taaggcgttt acgccgcatc cggcatgaag caacgtattc gatattagca atttggcggc
    30961 aacccaaagt tgccgattaa caattacttc gcatcggtcg ccgtgccgtt ggcgtgccag
    31021 tcaaatacgc cgaactcaaa gccctttaga tcgcctttct catcccaggt cagtggcccc
    31081 atcacggtat ccacggagtt cgcttttagg tatttggcga tttcagccgg atcgtcagac
    31141 tgattcaggc ccgcctgcaa agattgcagc gcggcgtagg tggtccagac gaatgcgccg
    31201 cttgggtcct gttttttcgc tttgatcgca tcaacaatgg gtttgttcgc cggaacctga
    31261 tcgtagttct tcggcttggt caccagcagc ccttccgctg attcgcccgc aatgttagac
    31321 agcgaaacgt tagccacacc ttccggcccc ataaactgag ttttcagccc tgccgcgcgt
    31381 gcctgacgca ggatttgccc catttccggg tgataaccgc cgtagtaaac gaagtcgata
    31441 ttctcttttt tcagacgcgc caccagcgtt gagaaatctt tttccccggc ggtgatacca
    31501 tcaaagaaca ccacgttagc attgcctttc ttcaggccgt cctgcaccgc tcgtgccaga
    31561 ccttcgccgt attgctgttt gtcgtgaacg atagcaatac gctgcggttt cactttctca
    31621 agaatatatt tcgccgccgt cggcccctgg tcggagtcca ggccggtggt gcgcaggatc
    31681 agctgatagc cacgggcggt cagctccggc gcggttgccg ctggggtgat cattaaaatg
    31741 ccttcgtctt cgtagatgtc agacgcaggc tgcgttgatg aagaacagag gtgaccaatc
    31801 acatatttaa tgccgtcgtt aacgactttg ttcgccaccg caaccgcctg tttcgggtca
    31861 caggcatcgt catattttac gatttgcagt ttgttgcctt taatgccgcc tttagcgttg
    31921 atatccgcaa ccgcctgctc tgcgccggta aactcctggt caccgtactg cgcaaccgga
    31981 ccggacattg cacccacgac cgcgacttta atatcttctg ccagagccat attgctgaat
    32041 gccagcgcga tacatcctgc cagtaacgct ttacccttta tgttcatcct gagaatcccc
    32101 attcttctgg ttattacgtg tgttgtgatg ttgttgttca gcactttatt tcgttttatg
    32161 catgactacc cgtgctttag cagcatactc tgctaaaaca tacccgattt ttatgatatt
    32221 ggaatagcta ttttgacagt ttattaacaa tctgcgtggg gattggcgtt ttgccggagg
    32281 gggaattgat tattgtagag gcccaaaaaa caaaaacccc ggactctcat ccagggttct
    32341 ctgcttaata gcggaaatta cgcttcaata gcggcgcgaa gttttttcat cgcgttcttt
    32401 tccagttggc gcacacgttc agcggaaaca ccgtaacggt cagccagttc ctgcaacgtg
    32461 gacttgttgt cttcgtccag ccagcgcgca cggatgatgt cctggctgcg ttcgtccaga
    32521 ccatgcatcg cgtcggtcag acggtttgcc gcctgctctt cccagttatc atcttcgatg
    32581 ccgtcggcaa agttagatga tttatcctgc agatagagca ccggtgccat cggctggctg
    32641 tcggaatcgt cgtcggaaga caggtcaaag gtcatgtcct gtgccgccat acgtgattcc
    32701 atctcacgta cgtctttgct ggttacgccc agttcacggg ccaccatttc gacttcatcc
    32761 tggttaaacc agcccagacg ctgcttggtt ttacgcaggt tgaagaacag tttgcgctgc
    32821 gctttggtgg tcgcaacttt gacgatacgc cagttacgca gaacgtattc gtggatctct
    32881 gctttgatcc agtggacggc gaaggagacc aggcgcacac ccacttccgg gttaaaacgg
    32941 cgcactgctt tcatcaggcc gatgttacct tcctgaatca aatccgcctg tggcaggcca
    33001 tagcccgcat aattacgagc aatatgaaca acaaaccgca ggtgagacag gatcagcgtt
    33061 ttagctgctt ccagatcgcc atggtaatgc agcttttcag ccagcgcccg ctcctcgtca
    33121 gccgacaaca tcggccacgc gttagctgcc cggatgtagg aatccaggtt gccaactggg
    33181 gctaaagcta aactttgcat tttgtcagtc attcaaatcc tctcaatcga tatcttctgg
    33241 cgcttcagtg gtagcaacaa ctgtgccaaa gcttaagagc aacgaggtta tcattcactg
    33301 ttttatcaga ccgtgatttt atccacaagt tcaatgcaag cttgtgaata aattacgcac
    33361 aaaatgtgac atagagatga aataccggga agagacaacg gggtctcttt ccctgctacg
    33421 gaacccattg cagggaaaga gtataacacg cttttattat tcaggcgtaa agtggcgtaa
    33481 atgttgtacc gtggcaagcc acgctgccac ccagccaatc atcgagcata ccagcagcaa
    33541 tagcaggcat tcatcgaacg ataaaccatt gatatcaaac ttcgttccga aaacctgtgc
    33601 cacttccgcc accgctgacg acaaacgcag taccagaatt tctgacaaaa ttaatgacaa
    33661 caatgcgcca gaaaatccca gcagcgcacc gccgtacagg aacgggcgaa ggatgaaacc
    33721 atccgtcgca ccaatcagtt tctgcacgtt gatggagtca cggcgagcaa agatgctcag
    33781 acgtacgctg ttgccgatga cgaggaacac tgccgccacc atcaacacgc cgatcatcgc
    33841 cgaaacgcgc ccaaccagcc cggtcaacgc cgccagacgg gcaaaccagc tgtcatccat
    33901 ccgcacttcg tcaataccgt taatctgcgt gatacgatca cgcagcgtat tgagtgattc
    33961 cgtcccctga aaatcgagtt tcgggatcac caccgccact gccggaagcg ggttttcttc
    34021 cagcatatcc agcgcaccac caaaaccaga ccagttacgg aactcgccca gtgcgtcttc
    34081 acgagaaaga tagttcactt tctccacgcc ttgctcggcc tgcaactgtg ccaccacgcc
    34141 cgcagcggcg tcatcgtcca gcgttttttg caagtaaaca gtgatttgcg gtgacggata
    34201 atactgcgtc gccgcctgat taacgttttt gtacaccata taacagacgc tgggcagcgt
    34261 cagggagatg gcgataacca tcaccgttaa aaacgtggcg aacggtttgc ttttcagatc
    34321 ctgcaatgcg ccgtggaagg catagcgcac ctgttcgttg aaaacgttgg ttttgcgatt
    34381 taccggtttt ggcgaggatt tcgcgcgttt cggtgcgtta cgaccgccgt cgcctgagcc
    34441 gccgaccgat ttacggaagc gatccagacg cccaccaaac tgccgaatat gattgattgc
    34501 atcgcgctta ttcatggccc acgcctccat gcaagtgacc atcgctcagg gtgagcatgc
    34561 gataggaacg ccgtgaaatc agattaatgt cgtgcgttgc catcaatacg gttaccccaa
    34621 cgcggttaaa ctcttcaaac agacgtagaa tcccttccga caacgcgtcg tccaggttac
    34681 cggtcggttc gtccgccagc agtaccgcgg gcttattcac caccgcgcgg gcaatgccaa
    34741 cacgctgttg ttcaccgccg gaaagctgaa tagggaagtt cttcgctttg tccagtagcc
    34801 cgactttatc cagtgccgcc gacacccggc gacgaatatc gtcaccgctg gcaccagcga
    34861 taatcagcgg gatcgccacg ttatcgtaga cagtacggtc catcagtaga tggtgatcct
    34921 ggaaaatcat gccaatctgg cggcgcagaa acggaacttc acggtttttc agacgcgtga
    34981 tgtcatggcc gctaaaccag attttcccgg cgctgggccg ctcaatccca cagatcagct
    35041 tcaggagggt acttttccct gcgccggaat gaccggtcag aaacgccatc tcacccggct
    35101 gcatatggaa cgtaacgccc tgcagcgcct gtctcccacc gagataagcc ttgctgacat
    35161 gttcaaagcg aatcattgtt aatcctctcg ggcaaaaagt gcctctataa agtcgtccgc
    35221 cttaaacgga cgcaaatcct caatacgttc gccgacacca atgtagcgga tagggatacc
    35281 aaactggtca gccaccgaga aaattacccc gcctttcgcc gtgccgtcca gtttcgttag
    35341 cgtgatgccg gttaagccaa cagcttcgtg gaacagtttg gcctggctta ccgcgttctg
    35401 cccggtgctg gcatcaatag tcagcataac ttcatgcggc gcttcaacgt cgagtttctt
    35461 catcacgcgg acgattttct tcaactcttc catcagatgc gatttgttct gcagacgtcc
    35521 ggcggtatcg gcaatcagga cgtcgatatt acgcgcttta gctgcctgaa tggcgtcgaa
    35581 gataacagag gcggaatccg caccggtatg ctgggcaatc accggaatat tgttgcgctg
    35641 accccagacc tgaagctgtt caaccgcagc tgcacggaaa gtatcgcccg ccgccagcat
    35701 caccgattta ccctgctgct caaactgacg cgccagctta ccaatcgtcg tggttttacc
    35761 cacaccgttg acgcccacca tcaggatcac gaacggcgtt ttatcttcaa cattcagcgg
    35821 ctcatcgact ttcgccagaa tctcgcccat ctcttctttc agcaggccat agagcgcctc
    35881 ggcatcacga agctgcttgc gggatgcgcc ttccgtcaga ttggtgataa ttttacgtgt
    35941 ggtttccacg cccacatcgg cgatcaacag ctgttcttcc agctcttcaa acagatcatc
    36001 gtcgattttt ttaccgcgga acaggctgat aaatccggaa ccgagatttt ctttggtttt
    36061 taacaggctg cgtttcaggc gcgcgaagaa gccttctttg gtcggttttt cttgctcctg
    36121 ggcaatttct gcaaccggct gctcttcttc tgccggagga actaccatca ccgcctcttc
    36181 tgccgcttcg gcagccagcg cctgtgcttc cagctcttcg tcggtgattt cttctttagc
    36241 cgcttcttct tctgccgctt cgacaatctc tacggtttcc gcttcagcct gccactcttc
    36301 tgacgaaacc tcttcggcgt tgacgtcttc cggcaacggc agctcttcac gttcgatagc
    36361 cactggctcc ggcgtttctt ctacgaccgg ttccggctgt gcaacaactt ccgcttcagg
    36421 ctgcgctttt tcactttcaa caacctgttc agtgacttcc acaacgtcgg cagcaaaagt
    36481 ttccgcctcg gcttcagtat gcgcctgcgg ctgctcttca acggcttgtt cagaagcctt
    36541 cgcaggctct tgcgcctgaa cgatttcttc tacaaccggt tgttcattct gaacttctgt
    36601 ctctttttcc ggggtctgct ctttttgacc aaagcccagc caggaaaaaa agccacgtct
    36661 tttttctttc gccatttgcg actacactcc tcgctgttgc ttcatggcac agcgttaacg
    36721 ctatgtacat agcagctaaa aaaatgatga aatagtctat cacttaactt aattcacatc
    36781 accgcctgca agtatgtgtt atctggcgga ttgagcaatt tatcatgaaa aaaccgaatc
    36841 attccggcag cggccaaatc cgcattattg gcgggcagtg gcgaggccgt aaactcccgg
    36901 ttcctgatag cccaggtctg cgccccacca ccgaccgcgt acgcgaaacg ttgtttaact
    36961 ggctggctcc ggtcattgtt gacgcccaat gtctggattg cttcgccggg agcggcgcgc
    37021 tggggctgga agcgttatcg cgctacgctg cgggggcaac gttgattgag atggatcgcg
    37081 tggtttctca gcagttaatt aagaatctgg cgacactaaa agcaggcaat gcacgcgtgg
    37141 tgaacagcaa cgcaatgtca ttcctggcgc aaaaaggcac accgcataat atcgtgtttg
    37201 tcgatccacc gttccgccgt ggcttgttag aagagacgat aaatttactg gaagataacg
    37261 gctggctggc tgacgaagcc ctgatttatg tcgaaagcga agtcgaaaac ggtctgccca
    37321 ctgttccggc aaactggtca ttacatcggg aaaaagtggc gggtcaggtg gcttatcggc
    37381 tgtatcaaca cgaagcacaa ggagaaagtg atgctgatta atattggtcg tttgttaatg
    37441 ctctgcgttt ggggattttt aatcctcaat ctggtgcatc ccttcccgcg cccgctaaat
    37501 attttcgtta acgtggcgct gatttttacc gtgctgatgc atggtatgca gctggcgcta
    37561 ttgaaatcca ctttaccgaa agatggcccg cagatgacca ccgccgaaaa ggtgcggatt
    37621 ttccttttcg gcgtgtttga actgctggtc tggcagaaga aatttaaagt taaaaaataa
    37681 ccttattgtt cgccaacaaa gctaacaaag cgcgttcctt tatagctcag cgtaccttta
    37741 tcgcccactg tcagggcgtg gtactgctgg gcgtcgaggc gaaacgtctg ctccattcct
    37801 ccgctttgcg gtttgaagct tgcctcatag cgcatactgg tgcctgccgg agtcacttcc
    37861 tgctggcgcg aacggcgatc gttaatcggt ttttcccgct tgttgctcac caccaccagc
    37921 ttttgctgga gcggagccat atcattatca gctttttccc gtcgctgttg cataaaacga
    37981 aacgatgcgg cgacgacaat taagccaatg ataacaataa agaaaagagg tggtttactc
    38041 atctttatcc ctcatcggaa aatgcggaat taagcatacc ctgccagtta tggtgttgtc
    38101 atccgtccac cctcgccact aaactggaag caagaccgta ggcattctgc ttacgaaaaa
    38161 ataacgaatt caaggaacta agatgctttg gtcgtttatc gctgtctgtc tttccgcatg
    38221 gctatctgtg gatgcatcgt atcgtgggcc aacctggcaa cgctgggtgt ttaaaccgtt
    38281 aacccttctt ctcctgctgt tactggcctg gcaagcaccg atgttcgacg ccattagcta
    38341 tctggtgctg gcgggactgt gcgcctcact gctgggcgat gcgctaaccc tgttgccacg
    38401 tcaacgtctg atgtacgcca tcggcgcgtt tttcctctcg cacctgctgt acaccatcta
    38461 tttcgccagt cagatgacgc tctctttctt ctggcctctg ccactggtgt tgctggttct
    38521 gggtgcgctg ctactggcga ttatctggac gcggctggaa gagtaccgtt ggcctatctg
    38581 cacgtttatc ggcatgacgc tggtgatggt gtggctggca ggtgaactgt ggttcttccg
    38641 tccgaccgct ccggcgctct ctgcgtttgt tggcgcttcg ttgctgttta tcagtaactt
    38701 tgtctggctg gggagccact atcgccgacg cttccgtgcg gataacgcga ttgctgcggc
    38761 ctgctacttt gccggtcact tcctcatcgt ccgctcactg tatctctgat aaaacttgac
    38821 tctggagtcg actccagagt gtatccttcg gttaatgaga aaaaacttaa ccggaggatg
    38881 ccatgtcgac acctgacaat cacggcaaga aagcccctca atttgctgcg ttcaaaccgc
    38941 taaccacggt acagaacgcc aacgactgtt gctgcgacgg cgcatgctcc agctcgccaa
    39001 cgctctctga aaacgtctcc ggcacccgct atagctggaa agtcagcggc atggactgcg
    39061 ccgcctgtgc gcgcaaggta gaaaatgccg tgcgccagct tgcaggcgtg aatcaggtgc
    39121 aggtgttgtt cgccaccgaa aaactggtgg ttgatgccga caatgacatc cgtgcccagg
    39181 ttgaatctgc ggtgcaaaaa gcgggctatt ccctgcgcga tgagcaggcc gccgacgagc
    39241 cacaagcatc gcgcctgaaa gagaatctgc cgctgattac gcttatcgtg atgatggcaa
    39301 tcagctgggg tctggagcag ttcaatcatc cgttcgggca actggcgttt atcgcgacca
    39361 cgctagttgg gctgtacccg attgctcgtc aggcattacg gctgatcaaa tccggcagct
    39421 acttcgccat tgaaacctta atgagcgtag ccgctattgg tgcactgttt attggcgcaa
    39481 cggctgaagc tgcgatggtg ttgctgctgt ttttgattgg tgaacgactg gaaggctggg
    39541 ccgccagccg cgcgcgtcag ggcgttagcg cgttaatggc gctgaaacca gaaaccgcca
    39601 cgcgcctgcg taacggtgag cgggaagagg tggcgattaa cagcctgcgg cctggcgatg
    39661 tgattgaagt cgccgcaggt gggcgtttgc ctgccgacgg taaactgctc tcaccgtttg
    39721 ccagttttga tgaaagcgcc ctaaccggag aatccattcc ggtggagcgc gcgacgggcg
    39781 ataaagttcc tgcaggagcc accagcgtag accgtctggt aacgctggaa gtgctgtcag
    39841 aaccgggtgc cagcgccatt gaccggattc tgaaactgat cgaagaagcc gaagagcgcc
    39901 gcgcccccat tgagcggttt atcgaccgtt tcagccgtat ttatacgcca gcgattatgg
    39961 ccgtcgccct gctggtaacg ctagtgccgc cgctgctgtt tgccgccagc tggcaggagt
    40021 ggatttataa aggtctgacg ctgctgctga ttggttgccc gtgtgcgtta gttatctcaa
    40081 cgcctgcggc gataacctcc gggctggcag cagcagcgcg tcgtggggcg ttaattaaag
    40141 gcggtgcggc gctggaacaa ctaggtcgtg ttacccaagt ggcgtttgat aaaaccggta
    40201 cgctgaccgt cggtaaaccg cgcgttaccg cgattcatcc ggcaacgggt attagtgaat
    40261 ctgaactgct gacactggcg gcggcggtcg agcaaggcgc gacgcatcca ctggcgcaag
    40321 ccatcgtacg cgaagcacag gttgctgaac tcgccattcc caccgccgaa tcacagcggg
    40381 cactggtcgg gtctggcatt gaagcgcagg ttaatggtga gcgcgtgttg atatgcgctg
    40441 ccgggaaaca tcccgctgat gcatttgctg gtttgattaa tgaactggaa agcgccgggc
    40501 aaacggttgt gctggtagta cgtaatgatg acgtgctggg tatcattgca ttgcaggata
    40561 ccctgcgcgc cgacgctaca actgccatca gtgaactgaa cgcgctgggg gttaaaggag
    40621 tgatcctcac cggcgataat ccacgcgcag cggcggcaat tgccggggag ctggggctgg
    40681 agtttaaagc gggcctattg ccggaagata aagtcaaagc ggtgaccaag ctgaatcaac
    40741 atgcgcccct ggcgatggtc ggtgatggta ttaacgacgc gccagcgatg aaagctgccg
    40801 ccatcgggat tgctatgggt agcggcacag acgtggcgct ggaaaccgcc gacgcagcat
    40861 taacccataa ccacctgcgc ggcctggtgc aaatgattga actggcacgc gccactcacg
    40921 ccaatatccg ccagaacatc accattgcgc tgggactgaa agggatcttc ctcgtcacca
    40981 cgcttttagg gatgaccggg ttgtggctgg cagtgctggc agatacgggt gcgacggtgc
    41041 tggtgacggc gaatgcgtta aggttgttgc gcaggagata aggcaaaccg atcgcaacat
    41101 tgagcgcgat cggtcccctc gcccctctgg ggagagggtt agggggtgag gaaaaggcgg
    41161 catcgaagcc aatcagcccc tatcaaccgc ctttacgaat caaataacga taaggcagtc
    41221 catccgtctc tttcgcaacc agttcgtgtt ccataaaggt acaaaaccca ggaatatcgc
    41281 gggtagtggc cggatcgtcg gcgataatca gcaacgtttc gccaggctgc atattgcgca
    41341 cggttttgcg caccatcatc accggttccg ggcagcgcag gccaagcgcg tcgagtgtgt
    41401 ggtcagggct ggaaaagaga tcggtcattt tcttctcatc acttaaaaaa acggcgctag
    41461 tttacgccct gtgagtccgt aagcaaacca ggttaacgat tgcgtgaaaa ttaaccattg
    41521 cattgtcaac gtaaagcagt atcatgcggc ggctcgaaaa aagggtaagc acgttattat
    41581 gttaaggtaa cagacgtgtc gtacgtattg ggttccctca ccccaatggt taatcaaaaa
    41641 ggtacaatat gaacgttttc tcgcaaactc aacgctataa ggcgttgttc tggttatcgt
    41701 tatttcatct tctggtgatc acctccagta actatctggt tcagcttccc gtctccattt
    41761 ttggtttcca taccacctgg ggcgcgttta gctttccgtt tatttttctt gctaccgacc
    41821 tgaccgtgcg tatttttggc gcaccgctgg cccgacgcat tatcttcgcg gtaatgatcc
    41881 ctgcgttatt aatctcctac gtcatctcgt cgctattcta tatgggttcc tggcagggat
    41941 tcggcgcact cgcccacttc aacctgtttg tcgcccgtat cgccaccgcc agtttcatgg
    42001 cctacgcgct ggggcaaatc ctcgacgtgc acgtttttaa ccgcctgcgt cagagtcgcc
    42061 gctggtggct ggcaccgaca gcgtccacac tgttcggtaa cgtcagcgac acgctggcct
    42121 ttttcttcat tgccttctgg cgtagcccgg atgcctttat ggctgaacac tggatggaaa
    42181 tcgcgctggt cgattactgt ttcaaagtgt taatcagtat cgttttcttc ctgccaatgt
    42241 atggcgtatt actcaatatg ctgttgaaaa gactggcaga taaatccgaa atcaacgctt
    42301 tgcaggcgag ttaaaggttc gttatcagag ttgtgataag atggatgaat gagccgttat
    42361 ggccgtttat cgaaaggaag aagtcaatgc gcaatctggt taaatatgtc ggaattggcc
    42421 tgctggttat ggggcttgcg gcctgtgatg ataaagacac taacgctacg gcgcagggtt
    42481 cggttgcgga aagtaacgct accgggaatc ccgtcaacct gcttgatggc aagttaagtt
    42541 tctcgctgcc agcggatatg accgaccaga gcggtaagct gggaacgcag gccaataaca
    42601 tgcatgtctg gtccgacgcc accgggcaga aagcagtcat cgtcatcatg ggcgatgatc
    42661 cgaaagaaga tctggcggtg ttggcgaagc gtctggaaga tcagcaacgt agccgcgatc
    42721 cgcagctgca agtggtaacc aataaagcca ttgagctgaa aggtcacaaa atgcagcagt
    42781 tagacagtat tatctccgcg aaaggccaga cggcgtactc ttccgttatt ctgggtaacg
    42841 tgggtaatca actgctgacc atgcaaatta cgctgccagc tgacgatcag caaaaagcgc
    42901 aaaccaccgc agaaaacatc attaatacgc tggttattca gtaagtttta agatgatgag
    42961 gcggcctcag ggacgtgttc cggaggccgt ttttttaatc gccacgtcag taataacgcg
    43021 attgcgacca gtcccgccgc cgccagataa atcaccggta cgcccgccca gctcatcacc
    43081 agcccagcca gtggtccagt cacgccaagc gataaatcca taaatacggt gtaagttgcc
    43141 agcgccgccc cctgattttg ctgcggaacc gcttttaccg ccactacacc caatgccggg
    43201 aacaccagcg aaaacccggc ccccgccagt aagacgccga ttttcgccat ccacggcata
    43261 gtcgccacgc caaccagtag caggccgatt atctcaacgc taaagcaaat catcgctacg
    43321 tttaagccac cgatacggtt aatgccgtta gggaataaca aacgcgtacc gacaaacgca
    43381 cagctaaaca gcgtcagcgc gaaagccgca ccgtcccaac ctttagcgtc ataaaacagc
    43441 gtgataaagg tggcgatgac gccaaatccg gcggaagcca gtgccagcgc cataccgtac
    43501 agccagacgc gcccaagcac cgcgcgaaac ggcagcggtt tgcctttact ggcttttacc
    43561 gtcggacgcg ggatcgccaa caaaatggcc accagcgcca cgcccataat gattaacgct
    43621 aacgcctgca agccgcccca gtgataaaac acgacgccta acggcgcacc catcgccatc
    43681 gccccgtaag tgacaatgcc gttccacgaa atcacccgcc cgatatgcag cgagccaacc
    43741 actccaacgc cccatagggt cgatcccgtt ccggcaaaac tttgcccaat cccaaggatg
    43801 acgcgcccca ggcaaagtaa taacaggctg atgacaggca gactggcggt taatcctgcc
    43861 gtcagatacc ccagaccgct caaaaagcag ccgcataaac cgaagacgac aatctttttg
    43921 ggtcccagca aatcggcgta acgtccggca tgagggcggc tcagcaaggt ggcgaaatat
    43981 tgcaggctga taaccaatcc tgcccagaag gcgctaaagc ccatcacatc atggacatag
    44041 cccggtaata cagcgagcgg caacccgatg gtgaggtagc tggcgaagtt aaacatgaca
    44101 atggagacaa tgcgcaaatt caggcgcaat ccgtttagcg cgggttcggc tacgggttcg
    44161 ggcatgagga tcaccacatt tttacaacag tgtttcattt ttaccatgtg ctgacgtgaa
    44221 aatcagcagt aagaatcaga atattgctgg cgtgactccc gcgctacact taacacaaaa
    44281 agtcacaagg aagccccaat ggaaacccct caacccgata aaacgggcat gcacattctg
    44341 ctcaagttgg cctcgctggt agtgatcctc gcgggcattc acgcagcggc agatatcatt
    44401 gtgcagctgt tactggcgct gttttttgcc atcgtcctca acccgctcgt tacctggttt
    44461 attcgtcggg gagtacaacg ccccgttgcc attacgattg tagtggtggt gatgctgatc
    44521 gcactaaccg cgctggtcgg cgtactggcg gcatcgttta acgaatttat ctctatgctg
    44581 ccgaagttta ataaggagct gacgcgcaaa ctttttaaat tgcaggagat gttgcctttt
    44641 cttaatttgc atatgtcgcc ggagcgaatg ctgcagcgga tggactcgga aaaagtggtt
    44701 accttcacca cagcgctaat gaccgggctt tccggggcaa tggcgagcgt gcttttgctg
    44761 gtgatgaccg tagtttttat gctgtttgaa gtgcgccacg tcccttacaa aatgcgtttt
    44821 gcgctgaata acccacagat tcacatcgca ggattacacc gcgcacttaa aggcgtttcg
    44881 cattatctgg cgttaaagac gctactcagt ttatggacag gtgtcatcgt ctggctgggg
    44941 ctggcgctaa tgggcgtaca gtttgcgctg atgtgggcag tactggcgtt tttgctcaac
    45001 tacgtgccca atatcggcgc ggtaatttcc gccgtaccgc caatgattca ggtgctgctg
    45061 tttaatggtg tttacgaatg tattctggtc ggcgcattgt ttttagtggt ccatatggtc
    45121 atcggcaata ttttagaacc acggatgatg ggccatcgcc tggggatgtc caccatggtg
    45181 gtatttcttt cattgttaat ctggggatgg ctgctcggcc cggtagggat gctactttcg
    45241 gtgccattaa ccagcgtgtg taaaatctgg atggaaacga cgaaaggtgg cagcaaactg
    45301 gcgattttac tggggccggg cagaccgaaa agtcggttac cgggatgagg cgacaagtga
    45361 tacgatacgc actttcattt tccattaaac gttggccctg atatgtatcg gatagttctg
    45421 gggaaagttt cgaccttaag cgcagctcca ctgccaccgg gtttacgcga gcaagcaccg
    45481 caaggtccac gacgcgaacg ctggctggcg gggcgtacat tgctttcgca cacgctttcc
    45541 ccgctaccgg agatcatcta tggcgaacaa ggcaaacctg cattcgcgcc ggaaacgccg
    45601 ctatggttca acttaagcca tagcggtgac gatatcgctc tgctgttgag cgatgaaggc
    45661 gaagtcggct gcgatatcga agtgattcgc ccgcgcgtca actggcgctg gctggcgaac
    45721 gccgtgttca gcctcgggga acacgctgaa atggacgccg tgcatcctga tcagcaactg
    45781 gaaatgttct ggcgcatctg gacgcgcaaa gaagccatcg ttaaacagcg tggcggcagc
    45841 gcctggcaaa tcgtcagcgt agatagcacc tatcactcct cgctgtcagt cagccattgt
    45901 cagctagaaa atttaagcct tgcgatctgc acccccaccc cctttacgct caccgccgac
    45961 agtgtgcaat ggatcgattc agttaactga tccgcccacc cgactgccca tctattgatc
    46021 cagaacaggt aatcagtatg acgaatactt aaaatcgtca tacttatttc cgccatctat
    46081 tttaatccat tggggttacc atgctctcca cactccgccg cactctattt gcgctgctgg
    46141 cttgtgcgtc ttttatcgtc catgccgctg caccagatga aatcaccacc gcctggccgg
    46201 tgaatgtcgg gccactaaac ccgcaccttt acacgcctaa ccagatgttc gcccagagca
    46261 tggtttatga accattggtg aaatatcagg cagacggttc ggtgatcccg tggctggcaa
    46321 aaagctggac tcattcagaa gatggtaaaa cctggacctt caccctgcgt gatgacgtga
    46381 aattctccaa cggtgaaccg ttcgatgccg aggcggcggc agaaaacttc cgcgcagtgc
    46441 tcgataaccg tcaacgtcac gcctggctgg agctggcaaa ccagattgtt gatgttaaag
    46501 cactcaataa aacagagctg caaattaccc tgaaaagcgc ctactatcct ttcctgcaag
    46561 aactggccct gccccgtcct ttccgtttta tcgctccctc gcagtttaaa aaccatgaaa
    46621 ccatgaacgg aattaaagcg ccgattggca ccggaccgtg gattttgcag gaatcgaaac
    46681 taaatcagta cgatgtcttc gtccgtaacg aaaactactg gggcgaaaag ccagcgatta
    46741 aaaagatcac ctttaacgtc atcccggacc cgactacccg cgcggtggcg tttgaaactg
    46801 gcgatatcga cctgctgtac ggaaacgaag ggttattacc gctcgatacc ttcgcccgct
    46861 ttagccagaa tccggcttac cacacccaac tgtcacagcc gatcgaaacc gtgatgctgg
    46921 cgctcaatac cgccaaagcc cccaccaacg agctggcagt acgtgaagct cttaattacg
    46981 cggtaaacaa aaaatcgctg attgataacg cgttatatgg cacccagcag gtcgccgaca
    47041 ccctgtttgc cccttctgtg ccttacgcta atctcggcct gaaaccgcgc cagtacgatc
    47101 cgcagaaagc gaaagagttg ctggaaaaag ccggttggac gctgcctgcg ggcaaagaca
    47161 tccgcgagaa aaatggtcag ccgctgcgca ttgaactgtc gttcatcggc accgatgcgt
    47221 taagcaaatc gatggcggaa atcattcagg ctgatatgcg ccagattggc gcagatgtct
    47281 cgctgattgg cgaagaagag agcagtatct atgctcgtca gcgcgacggt cgttttggca
    47341 tgattttcca ccgcacctgg ggcgcgccat acgatccaca cgccttcctg agttcaatgc
    47401 gcgtaccgtc gcacgctgac ttccaggcac agcaaggatt agccgacaag ccgctgattg
    47461 ataaagagat cggcgaagtg ctggcgaccc atgacgaaac gcaacgtcag gcgctgtatc
    47521 gcgacattct gacccgtctg catgacgagg cggtttatct gcctatcagc tacatctcga
    47581 tgatggtggt atcaaaaccg gagctgggta atatccccta cgcgccgatc gccaccgaaa
    47641 ttccgttcga acagattaaa ccggtgaaac cttaatgttg cgttacgtat tacgccgctt
    47701 tctgctgctg atcccgatgg tgctcgccgc ctcggtgatc atttttctga tgctgcgcct
    47761 cggtaccggc gacccggcgc tcgattatct gcgtttatct aacctgccgc cgacgccgga
    47821 gatgctggcc tctacccgca ccatgctcgg gctggatcag ccgctgtacg tccagtacgg
    47881 tacctggttg tggaaggcgc tgcatcttga ctttggtatc tcattcgcca gccaacgccc
    47941 ggtactggac gatatgctga acttcctgcc cgccacgctg gaacttgcag gtgcggcgct
    48001 ggtattaatt ctgctcactt ccgtaccgct cggtatctgg gcggcgcgcc accgcgatcg
    48061 tttgccggat ttcgccgtgc gctttatcgc gtttcttggc gtgtcgatgc ccaacttctg
    48121 gctggcattt ttgctagtga tggcgttttc ggtgtatctg caatggctac ccgcgatggg
    48181 ctacggcagc tggcagcaca tcattttacc tgcggtttcc atcgccttta tgtcgctggc
    48241 gattaacgcg cgactgctgc gcgccagtat gctggacgtc gccggtcagc gtcacgtcac
    48301 ctgggcgcgg ctacgcggcc tgaatgacaa acagaccgaa cgccgccata ttctgcgcaa
    48361 tgcctcgctg ccgatgatca ccgccgtggg gatgcacatt ggcgaattga ttggcgggac
    48421 gatgattatc gaaaacatct ttgcctggcc tggcgtcggg cgctatgcgg tgtcggcgat
    48481 ttttaaccgt gactatccgg tgattcagtg ctttacgctg atgatggtgg tggtttttgt
    48541 ggtctgtaat ttgattgtcg atttgctcaa cgccgcgctg gacccgcgca ttcgtcgtca
    48601 tgaaggagcg cacgcgtgaa ctttttcctc tcttcccgct ggtcggtacg cctggcgctg
    48661 atcattatcg ccctgctggc gctgattgcg ctcaccagcc agtggtggca gccgtacgat
    48721 ccacaggcaa ttgatttgcc gtcgtgcctg ctttcgccgg atgcgcagca ctggctgggc
    48781 accgatcact taggtcgcga tattttctcg cggctgatgg cagcgacccg cgtgtcgctc
    48841 ggttcggtaa tggcctgcct gctgctggtg ctgacattag ggctggttat tggcggcagc
    48901 gccgggttga ttggcgggca cgttgatcag gccaccatgc gcgtcgccga tatgtttatg
    48961 accttcccga cctcgattct gtcgttcttt atggttggcg tgctcggcac cgggctgacc
    49021 aacgtaatta tcgccatcgc cctgtcgcac tgggcgtggt atgcacgcat ggtgcgcagc
    49081 ttggtgattt cactacgcca acgcgagttt gtgctggcgt cacggctttc cggtgcgggc
    49141 catgtgcggg tgtttgtcga tcatctggca ggcgcggtga tcccttcgct gctggtgctg
    49201 gcaacgctgg atatcggcca tatgatgctg cacgtcgcgg ggatgtcttt ccttggcctc
    49261 ggtgtgaccg cgccgaccgc cgaatggggc gtgatgatta acgacgcgcg ccagtatatc
    49321 tggacccagc cgctgcaaat gttctggccg gggctggcgc tgtttatcag cgtgatggcc
    49381 tttaacctgg tgggtgacgc actgcgcgat catctggatc ctcatctggt gacggagcac
    49441 gcacactaat gccgcaacag attgaactac gtaatatcac gctacaggcc gcgcagccgc
    49501 tggtacacgg tgtatcgtta accctgcaac gcgggcgcgt gctggcgtta gtcggcggta
    49561 gcggcagcgg gaaatcatta acctgcgccg cgacgctggg cattttgccc gctggcgttc
    49621 gccagacggc gggggaaatt ttagccgatg gcaaaccggt ttcgccttac gccctgcgcg
    49681 gcatcaaaat tgccaccatc atgcagaacc cgcgcagcgc ctttaatcca ctgcacacca
    49741 tgcacaccca cgcgcgggaa acctgcctgg cgttagggaa acccgccgat gacgctacgc
    49801 ttaccgctgc catagaagcg gtggggctgg aaaacgccgc gcgcgtgctg aagctgtacc
    49861 cgttcgagat gagcggcggc atgttgcagc gcatgatgat tgcgatggcg gtgctgtgtg
    49921 aatcaccgtt tatcatcgcc gatgaaccga ccaccgacct cgacgtggta gcacaggcgc
    49981 gcatcctcga tctgctggaa agcattatgc aaaaacaagc gccgggaatg ctgctggtca
    50041 cccatgatat gggcgttgtg gcgcgtctgg cggatgacgt ggcggtgatg tcacaaggta
    50101 aaattgtcga acagggcgat gtagaaacgc tgtttaacgc ccccaaacat acggtgacgc
    50161 gcagcctggt ttccgctcat ctcgccctct acggtatgga gctggcatca tgaccttact
    50221 taacgtctgc ggcctttccc atcactatgc ccacggtgga tttagcggaa aacatcaaca
    50281 tcaggcggtg ctgaataacg tttccctgac cctgaaaagc ggcgaaaccg tcgccctgct
    50341 gggacgcagc ggctgcggaa aaagcaccct cgcgcggctg ctggtaggct tagagtctcc
    50401 ctcacaaggg aatattagct ggcacggcga atcgctggcg aaacttaatc gcgcccaacg
    50461 taaagccttc cgccgtgata ttcagatggt gtttcaggac tccatcagtg ccgtgaatcc
    50521 gcgcaaaacc gtgcgcgaga tcctgcgtga accgatgcgc cacctgctat cactgaaaaa
    50581 atccgaacaa ctggcgcgcg ccagcgaaat gctgaaggcg gtcgatctcg atgacagcgt
    50641 tctcgacaaa cgcccaccgc agttaagcgg cggccagctc cagctcgtct gcctggcacg
    50701 cgcgctggcg gtcgaaccga aattgctgat tctggatgaa gccgtttcta accttgatct
    50761 cgtgttacag gcgggcgtca ttcgcctgct gaaaaagcta caacaacagt ttggcactgc
    50821 ctgcctgttc atcactcatg acttacgcct ggtggaacgc ttttgccagc gagtgatggt
    50881 gatggacaac ggacaaatcg ccgaaaccca ggcggtggga gataaattaa ccttttcctc
    50941 tgacgccgga cgtgtgctac aaaacgcggt attacccgca ttccccgtgc gccgtcgcac
    51001 ctcagaaaag gtttaacgcc aatgcaacga gtcaccatca cgcttgatga cgatttactg
    51061 gagacgctgg acagcctgag ccagcgtcgt ggttataaca accgttccga agctatccgc
    51121 gatattctgc gtagtgccct ggcgcaggaa gctacccagc agcacggcac acaaggtttc
    51181 gcggtgctgt cgtatgtgta tgaacacgaa aaacgcgact tagccagccg tattgtttcc
    51241 acccagcatc atcaccacga cctctccgtc gccacgctac atgtgcacat caaccacgac
    51301 gactgtctgg aaatcgccgt gttgaaaggt gacatgggtg acgtgcagca ttttgccgat
    51361 gacgttatcg cccagcgcgg cgtgcggcac gggcatttgc agtgcctgcc gaaggaagat
    51421 tgagttattt ctaaaacaag gaaagaaaag atattaactg aacttaattt ataaaattaa
    51481 gttcagttgc atagatagat cctgtaaagg cagatattct gcgtaatgta cgtcgctaaa
    51541 atttataaat gattcaaacg ctcattcagt gtttcaataa tataggtatt aacagagact
    51601 tgttgctgcg cagccgcatt attaaggcgt tcactcagcg attctggata gcgtaacgta
    51661 aaggtcttaa tcttttctgt acgcgcatac ggctcaatac ccgcagcttt acaatcctca
    51721 agatactctc gcagggaaag ctctccttct ttttgcagcc cctgaatact gtcagagaca
    51781 aaatcacaat aacctgacaa gcccaggaat ttgccacgaa atgcattcag ttctggcacg
    51841 taggtaataa cagctggttg cccggcaatc tccatagagt ttggcgtctt cagtttgatc
    51901 atggttttac ccctatactt agtaaccagt cacacacgct ttctacagcg cctttatcgg
    51961 tatctggcga cggatgaggc cgatggaaac acgcaacgct catatttagt atgaacttgc
    52021 aacgcgaacc tcttccttcc ttaatttctc cgcctaatgc tttaaccagt gactctatat
    52081 cagaccactt gatcccttga ggaacaggag ttttaaatat ttgatccaat gtattttttt
    52141 gtttcttccg taaagcctgt accttggttc tcatggcatg tccttatgac ttcactttat
    52201 gacatcacag tatgaagtca agtttccctt ccttacgcca ttgtcccaat cgtcttcctg
    52261 aatcgcagca gtgcgatggt gaaaaacgcg ccgccaattg ccatcagcgt caggaactgc
    52321 ggccagacga tttcgaatcc ggcaccccgg tagaggatgg cctgcgcgag gctaacaaag
    52381 tgtgtcgtcg gcatggtcag cataatgtcc tgcaccatct gcggcatact ttcgcgcggc
    52441 gtggatccac cggaaagcat ttgcagcggc agcagcacca gaatcaccag cagccccagt
    52501 tgcggcattg aacgcgctat cgtccccata aaaatgccga ttgacgtggt ggcaaacaga
    52561 ctgagcgcca cgcccagcat aaacagcggg atcgagcctt caatcggtac gcccagtaca
    52621 cctttcacca tcagcaccag cgataatccc gataccacca gcaccaccag ccccatcgac
    52681 cagatcttcg ccatcatgat ctcaaacggc gttatcggca tcaccagtaa atgttccacc
    52741 gtgccgtgtt cacgctcgcg gatcagcgcc gatccggtca atacaatcgc cagcatggta
    52801 atgttgttga tgatcgccat caccccgcca aaccacgcgg gatcaaggtt cgggttaaag
    52861 cgcatccggg tttccagcga taccaacggt tcgctgttat cacggtagcg cgcgacaaag
    52921 ctgttcactt caccgttgat aatattctgg atatacccat tgccggtaaa tgcctggctc
    52981 atgcgcgtgg catcgacgtt cacctgaata tccggctggc gtccggcgag gacatcacgc
    53041 tgaaaattag gcggaatatt tatcgcgaag gtatagcgtc cggcgtccag tccggcatcc
    53101 atctcatcgg cggtgatcat ctccggtggc aaaaaccacg gacgatagaa gctgttaacg
    53161 atccggttcg ataactgcga ttgatccata tcggcaatgg cgatcggcgc gaggttcaac
    53221 gatcctggcg taacggtcgc tgacgaatac accgacaccg taaacgagaa gacaatcagc
    53281 gtcagcatcg ctttatcacc gagcagactg cgcaactctt tgatacccag attaaaaata
    53341 ttgcgtaaat ggcgcatcat ccctcctgtt ttttcagcag caggatgctt aagcccatca
    53401 ccagcgggat ggctatcagc aacgggataa aaagttgcca caaatcagtc agatccagcg
    53461 ctttcgagaa cgttccgcgg gcgatagtca gaaaatgact ggtcgggtaa acctcgccga
    53521 tccaacgtcc aggcccttcc agcgaagcta ccggatcgat catcccggaa aactgtgtcg
    53581 ccgggatcaa tgtgataatc gccgttccga aaatggcggc aatctggctt ttcataaagg
    53641 tggagatcag cagccccatt ccagtggcaa tgatgatata cagcagcgcc gccaaggtga
    53701 gcgtcaggaa actgccttta tgcggtacgc caaacacaaa caccgacagg ccgcagagca
    53761 ggaaaaagtt cagcatcccc agcgcgatgt acggcagctg tttgccaagc aaaaattcgc
    53821 tacgggtggt gggggtcacg taaaggttga taatcgaccc tagctctttt tcccgcacca
    53881 cgctaagggc gcttagcatt gacgggatca tcatcagcag aagcgggatc accgccggaa
    53941 caatcgctgg caggcttttt acgtccgggt tatagcgata acgcgtctca atgttcatca
    54001 gcccgctttg gctggcgggt gtcgattgtc ggctcgccac atcctgaagc caactctggt
    54061 gcattgcctg cacgtaacct tttaccgttt cggcacgact gggcatcgct ccgtcgatcc
    54121 agacgccgag ttccacaggc gtaccacgcg cgatatcgcg cccgaaattg ggcgggatct
    54181 caatggccac cgtgatatcg cccgcacgca tccgacgatc aagctcgtca taactggtga
    54241 gcggcggctg ttcgataaag taacgggaac cggagaggtt gagtgtccac gcctggctac
    54301 tgacggtctg gtcgcggtcg agcaccgcaa agcgcaggtt ttccacatcc atactgatgc
    54361 cgtaacccat tatcagcatc aggatcaccg ttcccatcag cgccagcgtc gaacgtactg
    54421 gatcgcgtcg cagttccagc gcttcgcggc ggctgtagct aaacagacgg cgcaggctaa
    54481 atccctgacg cggcgcgtgg gtggtgtcgt gtaccacggg cggcgcttcg gcttcgttgc
    54541 tctgccctgc cgcttcctgc aaataggcga taaatgcctc ttccagactg gcggctccgc
    54601 gtttctcaac cagttcctgc ggtgtaccgc tggcaagcac ttttccggcg tgcatcagtg
    54661 agatgcggtc gcaacgttcc gcttcgttca taaagtgggt ggagatgaag atagtcactt
    54721 tgtcctggcg cgagagatcg accatcaact gccagaacat atccctcgcc accggatcga
    54781 caccagaagt aggctcatcg aggattaaca tctccgggcg atgaatcacc gccaccgcca
    54841 gcgaaagccg ctggcgaatg ccgagcggca atgactccgg cagaacatct tcaacgtcgt
    54901 tgagcttaaa acgctcgctc atttcagcca ctcgcgcggg aatttccgct tccgggatgt
    54961 gaaacaaacg ggcatgtaac tccaggtttt gccgcacggt gagttcgtta tagagcgaaa
    55021 acgcctgcga catatagccc acacgacggc gggtatcgat atcttttgga tcaaccggtt
    55081 gcccaaatag ccacgcctca ccttcgctgg cgggcagcag tccggtaagc attttcatgg
    55141 tggtggattt accgcagccg ttcgaaccaa ggaatccaaa aatctccccg cgtggaatgc
    55201 ggaaattgac gtgatcaacg gcaacgaagg aaccaaaacg catggtcaga tcgcgcgctt
    55261 cgatggcaat ctctgcgttt tcaggttgat acggtgggat cactaccgcc tgatgcgcct
    55321 ggcgttgcgc ttgcggtaac agattaataa atgcttcttc cagcgtggcg ctttgcgttt
    55381 gctgccgcag ctcttcggcg ctgccggttg ccagcacttc tccggcattc atcgctacca
    55441 gccagtcgaa gcgttcggcc tcttccatat aggcggtggc gaccagcacg ctcatattgc
    55501 tctgccgctg gcgaatactg tcgatcagat cccagaactg ggcgcgggag agcgggtcaa
    55561 cccccgttgt tggctcatca aggatcaaca gttccgggtc gtggattaac gcgcagcaca
    55621 gcccaagttt ttgcttcatc ccgccggaga gtttacctgc cggacgatcg cgaaacggtg
    55681 ctaacccggt gctggtcagc agctcattaa ttcgcgcttc ccgctccgct ttatcgtgac
    55741 cgaacaggcg agcgaaaaaa tcgacatttt catacaccga caaggtgtgg tagaggtttt
    55801 tgcccagccc ctgcggcatc caggcgatgc gcgggcagac gtctcggcga tgcttcgggt
    55861 cgtgcatatc gccacccagc accaccacat tgccctgctc aatgacgcgg gcaccggaaa
    55921 tcagtgacaa taaactcgac ttcccgacgc catccggacc aatcagcccg accatgcaac
    55981 gggccggaat atcgagagtg atattgttca gcgcaacggt ttttccataa tgctggctca
    56041 cgcccgccag ttgcgcgaca ggcgggacgg gaaccagttc cagatgcgtc attgcggcaa
    56101 cctcaccacg aggtcgtcag gccacggaag ttcttcattc acccgcaccc acgctacgcc
    56161 cggtaaaccg gttttgacat attccagatg ctgctggagt aattccggtg ggatacgcgc
    56221 tttgacgcgg aacatcagtt tcagccgttc atcgctggtt tcgacggttt ttggcgtgaa
    56281 ctgggcgaca ctggcgacaa aactgatggt tgcaggaata cgcagatctg gcgcggcatc
    56341 gaggatcagc cgggcttcac cgcccagttt cagcgtgccc gcctgttcgg ttggcaggaa
    56401 gaaggtcata tagacgtcgc tgagatcgac catattcagc acccgaccgc ctgccgccag
    56461 cacttcccct ggctcggcaa cccgatactg cacgcgtccg tcacgcgggg ctttcagttc
    56521 actgtcatcg atatctgcgg caatgcgccg ttcagtggct tgcgctgctt cgacccgagt
    56581 ttgcgcctga atgatattgg tgcgtgctgc ttctatagcc gctttagaag ccgaaacctg
    56641 ggctttcgct gattccagcg cagctcgggc gctctcagcg gcggcgcggt cgtcatccag
    56701 ctgttgcgca gaaatagccc ctcgttgggc gagcgagcgg gaacgcgtat gacgtttggc
    56761 aacagagtcc agttcggcct ggcgttgatt gaccagcgac tgtgcggcac gagtttcact
    56821 ttgtcgttgc tccagcaaag cctgcgcggc ggcaacggcg ctttgtgcct ctttgatttg
    56881 cgcgatggct tccagtcgct gttcctgcaa cacgcgagta tccatcttcg ccagcacttc
    56941 gccttcgcga acaaactggc cttctttcac cagaatggtg tcgatacgcc cggcaatttt
    57001 gctggcgata tccacttccg tcgcttcaat gcgcccattg ctgacagcaa agccttccgg
    57061 cacacccgcc gggcgcaaca gccaccacgc cacgacggca gccaccgcca gtgccccgac
    57121 aacccaccac gccagatggc gcttactctt atccataatc gacccgccat aatccctgta
    57181 agcaaacgac gcgtgttaca actgccgccg aacgacgttc agcggattct tccatccggg
    57241 ataagaagga aacgacaaat acgaccagcg cgcagagcat tgagcgccaa cggggaaggc
    57301 ttagtgaaaa cgggtagtaa tgttgtcatc atcagcattc ctggccgtaa atgaaacacg
    57361 catactcata agtgtgatct ataaaactgg ctgacaaaac ggtatccatc ctgttggatc
    57421 gcatttcacg cctgttcagt tgcaggaaca gagcattcct ttttacgttt tatcaacagt
    57481 ctcaaccaga tagtaacatt aatattcatg ttgtctatgg ttcagagctg taacattgtg
    57541 cgccttctaa tcaaaggcat aagtccattt ctgttttttt catgctggta agacttccag
    57601 ggagtggtta tctctggccc tgcaccaggg ttaatttggc aattatgcat gctgaacatt
    57661 cttccagcat gtttactgtc acgggcgtta agacctaaaa ctgtccccgt ttcacctatg
    57721 cgttctctcc caccatttcc gttatgtgca acaacttgat ttatacctaa catttgtgaa
    57781 catgctaata gtgcttcaag tttaggtctg taatctgtcg aaacagaaaa ataacgatta
    57841 aaggatcgac aagagatagc ccggctagtt ttaaactttt tgccattaat tatagcatga
    57901 tgctttttat taataatttc cgctaattcg accggatcga gagcattggt catttcatca
    57961 atagagacag ttatcggtcc tataatgtaa cgttttcgct gatcatcaaa agttatccca
    58021 tgatgatttg ccattatttt gtatttgggg tcatagaaac atacaggata atcttttatc
    58081 ccgtcgtagg tatcctcatc aagtggtttc atgcgcgtga agttttgcat atagttacca
    58141 ttgcaattcg tttcatgatt tccagctaat acatgaacat tttcattgat actcattatg
    58201 cgttttaaca aatcgatgat aaattgatcg ccacagatat tactaaatcg atcgccggtt
    58261 tggtcgccaa gaaacgtaca tggcgtgact gcgtctttat taataaccaa ataaggctga
    58321 agactttcta aaacattatc tcttctgttc agtatactga ttattgtatt aacattagtg
    58381 tcaagttgct cattaaagtt ctcccttagc aatacgttga gaacttcatc ttctatatta
    58441 agcaactggc taaggaaatt ttctccttca gccatgattc ccaaatgtcc tgttgcaaaa
    58501 agcgcgtaca aaactgcacc gactgagcca tcggtatcgc caaaataggt cggcctggta
    58561 cttgtgggat caacaggtga acaattggag aaataggttc tgtcagcgaa ctggggtttt
    58621 ataattgttt cgaatttaat atcaaataca ttgtattgcg atatttccca gttccagatg
    58681 ggtttgtacg actcagtaac tgcaacatca ttagcattat catttactgt catattatta
    58741 agtaaataaa caatgctgca ccaattaata tcggtattat caagagggca accgtaattt
    58801 ttactattaa ttgtcacaat aaactgataa cagttattcc tacgtagaat atgagcgcgc
    58861 atgcccgata gcatactata tttttctgaa tgattcgttt gtatggtaaa tgaaattttg
    58921 tcatcttgaa tagattgttg gaaacgttct atttgccaat catcaatttt ttccacaact
    58981 tcaccgagca ttgtcctggc aatacttgat atatcatcac ctataccatt tgcaatatac
    59041 ccaaacagtt ctttagtgaa gttggtaaca tgctcttgca ccatatcatt tgttgcgatt
    59101 gtcgttgtac tgccattagt gcctgccaca gttccaattt tcatctttta cctcatatta
    59161 ttcaccgcga ggaatacata gttaacattt tatcttatga catacacctc atattcgatg
    59221 gggaaataat tatattttgc acaaatcatt cagccttaaa gcataatatg aaatttctta
    59281 tctcatagca gagaaagtcc ctaaatattg tagaggcgtc atcagtacat acctctacaa
    59341 tcgattaaga aaacctatga aatactggca aaattaaaaa gagataccac agcactcatg
    59401 aagttttatt gaggttgaaa acaactaagc cctgggacac cttttttatc accacgttcg
    59461 ttaggtgcta tcttccaaga atttagcccc atggtataat tatcaatttt ttgatgctgg
    59521 tctaccccta atccataatg gccaaatttc tgtttagttt ttttgagcgt ctgtccacat
    59581 ttgaagatat cttcaggttt tggtctgaaa gcagtagtgt tattaaaata attttcatat
    59641 acaggtgtgg gtgctatagc attgcttcta attagatgga gaagatgatc atccatgtgt
    59701 tgtttatgtt ttttattaca tatatttgct aattcctcag gatcaattgg gtctttcatc
    59761 tgattaaatg gaacctgcaa agcaccgaga caataacatt gattttcttc atttcttatt
    59821 attccgtgat gactggttaa aactttagtt tctgagttat aattacaaac atcgagcgtt
    59881 ttaattaagt tatacgtatc accggcagag agtttataat tttctagtcg tgcagtatag
    59941 ttaccattaa aattgatttc atgatttcca gccagaacga tgacattttt attaatacgg
    60001 ctatctttat taccctccat atttcgcata gaatttagca aagtaaggat atatttatca
    60061 ccaaaaatag tactaaatct atcgcctgta tgatccccaa ggaagataca aggggttatc
    60121 gcatcgtcct ttaaaaaaac atgttgatgc aactcattca atatcatgtc tctcgtattt
    60181 aataaattgt agagcaattt attattttta tgtatgtatg cagatgaagc catgtcttcg
    60241 tgttttaata attcacataa taaactccaa cccttttccc tgatgcctat atgccctgaa
    60301 acgagcaatg aataaagtac agcgcctact cttccatctg tatcgccaaa ataagccgtg
    60361 ttggcatttt gtgggttaat cacaccacat tgtttataaa agtctatttt agaaaacaca
    60421 gatttaacat agctatcaaa cttatcctta ggaaacgtct ctttagagac atcccaacgc
    60481 cagtcagctt taaaattacc gctaagaaga gtaaagaaat tttcaggtat gatagaaagc
    60541 ccattaactt tataaattat gtctgattta tctccggaga aattaataaa ataataacca
    60601 tccttacact caatcttaac tgtaatggga gacgataatt ttatcacatg gttattgtct
    60661 ggtataattg taaagataaa cgtgttagta gtattatcgg caaaactatt atattcttga
    60721 atatctatag atggctcact attgtaaaac catattttct ccatctgaag actatgttca
    60781 tttaccctcc agtgaccatg ttgcaggaaa tagatgatat gcatgccaaa atcgttatct
    60841 atttgcgaat cattatccag cttatcaatt tcaactatct ttgaacgatg tgttttatca
    60901 accttgctat ggtgttgatg attattaact ttatctgaat gcttatcttt atggtttgaa
    60961 gggatgtact taaatatatt gtcaattgct ttcataaata acccgcgata tttttgacct
    61021 gttagtaaaa aataaatgat acatgtctga aagacatatt tacgacgata taaaacaata
    61081 atacttatat gttcgctgat tttaagcacc gagactatcc ggtcttttac taaataatgg
    61141 aaaacaatcc atccctggaa gacaattgta aaaaaaaaca acagaataag aattaatctt
    61201 aggataaata tttatttatc atggcctttt gaccgctaaa aaatggcact cggcacagta
    61261 aaccccgcta tagttatgct atgcatttgt ctttaatgat atctcgtcgt tatattaaca
    61321 ggctgaaatt atcattaatg cacatttcaa tattagcagg gatacctgag gagtttaaag
    61381 tgaacatata tatcgggtag cttttcaaat taatcccttt gattataggc ttaatttgca
    61441 tcgcgttagg tggctttgtg ctggaaagtt cagggcaacg tcacgggtca tgtgctgatt
    61501 tctctggcgg ccatatgcct ggcattattc actaccgcat ttattatcat ttcgcaactc
    61561 acgcgcggcg ttaatacgtt ttacaataca ttgttcccca ttattggcta tgcggggtca
    61621 attatcacca tgatatgggg ttgggcactg ttagcaggta atgatgtgat ggcagacgag
    61681 tttgttgccg gccatgttat ttttggcgtt ggtatgattg ccgcatgtgt atcgacggtg
    61741 gcagcgtcgt ccggtcactt tctgctcatt cccaaaaatg cagcggggag caagagcgac
    61801 ggaacaccgg tacaggctta ttcttcatta atcggtaatt gcctcattgc cgttcccgtt
    61861 ttactcaccc tgctcggttt catttggtct attacgctgt tacatagtgc cgacataact
    61921 ccgcattatg tcgccttggg ttaaccgcaa tctgtgcctg tctaattggc ctagtggcca
    61981 caatcgtcca tcaaacacgt aatacgttct catccaaaga gcactggctg tggtgttatt
    62041 gggttatttt tctcggctca gtcacggtgc tgcaggggat atacgtctta gtcagttccg
    62101 atgcaagcgc ccgactggct cccggcatta ttctcatttg cctcggaatg atctgttaca
    62161 gcatattctc aaaagtctgg ctactggcac tggtctggag acgtacctgt tcgttagcca
    62221 acagaatacc gatgataccg gtatttacct gtctgttttg cttgtttttg tcatcattcc
    62281 tcgcggaaat cgcacaaact gagctggact actttattcc atctcgggtt ctggttggat
    62341 taggcgcagt atgttttaca ttgttctcta ttgtttcaat attagaggcc ggttctgcga
    62401 aaaaaaatta aaatttacat aatcccagcg ttattcatat cttaacgctg gttttttacg
    62461 aacttagtga aataatgtag aatataaaat tataatttta tattggagca catgtgttaa
    62521 tgatcaaccc cgggatacta aaaatataga cattattaaa cttgcttagc ctcgcattgc
    62581 ctttacctgt taaagatgca tcgagaaaat tgagtctgaa gttctcatcc tttatctgat
    62641 tttccagtcc cgaaactttg gccttagcat tacctgagat ccgtccgtta ctatctattt
    62701 tgtaggtaaa aaaatacgaa tgggaaaatt tcctcggtgt atcttcatca gttaagccaa
    62761 caatcgtaaa actaccgcta ccatcttttt ttaaaatgac tgaaaggtta gcagagtagt
    62821 ataaagaatg atctgtttta gcatttcttg ttaacgtcga cgtacattca agagacgttg
    62881 tgctaccatc acgagccttc cagagagcaa aaaaacacac ctgtagcaat gtgaaaacaa
    62941 gtattaatat aaaatacatt ttcttattag tcatataata gcacctctga acaaattgca
    63001 taagtttcgt cattagagga ttttatacat cttgataaat accgctgccc ttccccaagc
    63061 aaataatgca tactatttac atcatatata aaatttgcac ctggcacaca gggtaaatgc
    63121 tttttcgaaa tttcagcggc ctctatttga gatttagcga tgccaatctc gttaagattc
    63181 tctgaaagat ataaatcaca ggtgtcaatc ttacctgatt taaccaatgg ataatccgtt
    63241 atactgtagg tatctttaaa agccacggca aacatttcaa taataacaat tactaccaca
    63301 agcgaaatca aaatgcgaaa aagaggaata ctccttttgc gctctacttt ttcaacaaca
    63361 tcttcttgta aatgagaatc atcataagat tgtttactta ttgttatgtt ttcactgatg
    63421 taaaagccta ctttgggtat cgtaagaatc acattttctt caacaccaat atttttaaaa
    63481 cttttgcgta acagggatat atattggttc aatgtgttgt ttgacagatc aaatgaaaat
    63541 ctctccaaaa cgctatcaat gattgagttc ctgtcaaccg ctccaccatt actttccagt
    63601 aacactgcca aaatacaccc cgccgtagct ggaaaattta tctcatcctg ggtatttaca
    63661 ttcgagagcg agcatttgtt cggatcaaaa ataatcgcgt cattgataat gaaaaaacat
    63721 ctccgctcca tgcaggcaac aacacaaaat aagattttgt cattttagcc tattcctggt
    63781 ggaactgaca caagaaacta aaatacaaat ataaccctga aaaagtcata taaaacttat
    63841 atccacagaa taaaaaatca aacatgtcag ttttatcaat ataaagtatc gcaaggcgaa
    63901 aagtgatgca agaatactcc taagtaaatg aaagcgcacc acatgaaatt atcaacacat
    63961 gtaatttcat cattaggtgc tatcgaaaat aactcaggag ctacttaaga tgaataatgt
    64021 cttactcatg agacccgatg actcagaatt tatgatacct gatgactggt cagggctaaa
    64081 cgcaaaaggc attacatacg aacaagataa tagagtgaca gagtcttttc tgccttttaa
    64141 atctttttat tgttcaccgg tgtgcacgac agttgaactc atctcataaa ataattgttt
    64201 tgctatgggt atccgctacc ttctggaaaa gaaaagagac ttaaggctaa atcactttaa
    64261 agccttctct cataaatgcg agacaaaacc tgacgtgtta attatgaccg ggtgcaccgt
    64321 cgctgatact tatagtatat tctcacgcat tgttgaagaa aatataatga aaacgatgac
    64381 acctatcgtc atcattactg ggcatcactc agaagatctt tgttctctgg catcgaaact
    64441 gggaggagtt aaaataatat caccgacaga aaagattgca aaaatagaaa acgctatcaa
    64501 tgatgctttt aacagcaagc ctaaaaaaat aagattttta acagatatac tctctcctca
    64561 acaaattgag accatgaaaa tggttgcgca gggtagatcc gttaatgaga tttcagccgc
    64621 aatgaatatt agcatcaaga ccgtgtatgc acataaagtt cgtgctatca ataagctggg
    64681 aattaagaaa aaagtttatg aagcgctttt ttataaagcg ctaattcagt tcaactgatg
    64741 taaaaatcac cctcgcttaa taaataatat attctatgtc tttttttata agttaaaaaa
    64801 ggcgggatat caaatcccgc catgaaatta actaatcatc tgcattattc tgcgtagata
    64861 taagcctggg tacggatggt gtaagagtct ggctcaacaa cctgttcttt catatgggaa
    64921 acgacgcgat aggcaccacc tgttccagta tttttcagca tcatataacg cttaccgcca
    64981 atctcttcgg aggcagctga aggaggatcc tgctcaagcg ttgtcaggga aactttcagt
    65041 ttatggtccg ggttattgtc accagaaatt actgcatccg ctgaagtact gctctccgca
    65101 taaatatcat ccgggaaagt tacagcatag tcgtggctct gcccatcaga tgcgccaaca
    65161 atacctgttg ccaaaggcgt ccatgcagtt aacgtaccgc tatatacccc tttcactggt
    65221 tgaatggtat tattaacaac gataggcttg gtcaaagtaa gattagctga cgcttcagta
    65281 ccctggctta ctaaagttcc cgcctgagaa gcaaaagctg tccccgccag catgattgct
    65341 aacaatgttt tcttcattat attttctcca tgattttcat ttacactgca aaatgcagtt
    65401 tctttctttg catttacgtg ctatcgcgaa aatgcttcca ccaaataatg gaaacatctt
    65461 cacaaaaata aattatgagt ttcttaattt agaaactata aacatgtcgc cgctatctgg
    65521 tataaccctg atgcactttt cttcgcagga agttggtatt ttgcgataca ggattgatct
    65581 ttagaataac cccatttcac attaaactcg ccttccgcac tcaagccaga aacataagca
    65641 ataccatctt cacctacaat gccggccata ttttcagtgt ctttgctata actaatcaca
    65701 gcgccgaagg ggatgggttt accctctttc tgttttaagg ttactaatcc tttatccccg
    65761 ctcttcactg ccaatgtcgc aggcacaacg gctccagctg tcggtacaac caattgacta
    65821 gtttgtggta agtcgtaatt cttacctatc gttttttcat ccagaataac atcgtctctt
    65881 cggtatggct gcactggcag tattgcatac ccacgaaaat cagtactcag tcccggccag
    65941 ccattaacgt taacccccgt tgctccgggt gcctctataa gggctaatgt cccatccaca
    66001 ttttgaccaa atgtaagtcc atcccttgta ataagtatcc caccgcttaa atttgcacct
    66061 agctgtttca tgttttcgct ggagctgtaa tggctactaa actgaccata tgatccattt
    66121 ttgccaactt gcacaatgtt catcgtgctc ttcggttgtg actgatgagc gttagttata
    66181 tttgtcgcta catcccacca tacaccacca ctattctgac ctgataatga aacctgatag
    66241 ttattaccct gctctcccga acgactcatc tgtgaagacg cataaatttg attttcgtca
    66301 cccagcgata atggcactga aatacccacg cttaatttcc gatctacttt ccatttatgc
    66361 gatgtcagca agtcgttttt tgcccagtta atggagaatg ttatgcctct ccataccgta
    66421 ctataactca ggctggtaga ccgcgtgttt cctgtgcccc gggaagaatt atacctcgtg
    66481 gtattgaagt tcaaattccc gatattacca aggggctggg ataaggatat cgtagagcca
    66541 tcgtgactat tcttatctct ttttctgttc cgttcatcct gtgcacttac tgcttcagtc
    66601 agtgtggcat aacctgatgc ataacggttc gtggttatag acaatagact ggccccctga
    66661 atctccagac aaccagtatc acttatttaa gtgatagtct taatactagt ttttagacta
    66721 gtcattggag
//
LOCUS       DAJTSF010000008        66032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000008 DAJTSF010000000
VERSION     DAJTSF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.008"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="OO335_000575"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="OO335_000575"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6001081.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO335_000576"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO335_000576"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6001082.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO335_000577"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO335_000577"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6001083.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO335_000578"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO335_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6001084.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO335_000579"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO335_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6001085.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO335_000580"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO335_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6001086.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="OO335_000581"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="OO335_000581"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6001087.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10139
                     /gene="mrcB"
                     /locus_tag="OO335_000582"
     CDS             7605..10139
                     /gene="mrcB"
                     /locus_tag="OO335_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6001088.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10359..12602
                     /gene="fhuA"
                     /locus_tag="OO335_000583"
     CDS             10359..12602
                     /gene="fhuA"
                     /locus_tag="OO335_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6001089.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12653..13450
                     /gene="fhuC"
                     /locus_tag="OO335_000584"
     CDS             12653..13450
                     /gene="fhuC"
                     /locus_tag="OO335_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6001090.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13450..14340
                     /gene="fhuD"
                     /locus_tag="OO335_000585"
     CDS             13450..14340
                     /gene="fhuD"
                     /locus_tag="OO335_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6001091.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14337..16319
                     /gene="fhuB"
                     /locus_tag="OO335_000586"
     CDS             14337..16319
                     /gene="fhuB"
                     /locus_tag="OO335_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6001092.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="OO335_000587"
     CDS             complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="OO335_000587"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6001093.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17859..19280
                     /gene="clcA"
                     /locus_tag="OO335_000588"
     CDS             17859..19280
                     /gene="clcA"
                     /locus_tag="OO335_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6001094.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19304..19369
                     /locus_tag="OO335_000589"
     CDS             19304..19369
                     /locus_tag="OO335_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001095.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19362..19706
                     /gene="erpA"
                     /locus_tag="OO335_000590"
     CDS             19362..19706
                     /gene="erpA"
                     /locus_tag="OO335_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6001096.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19753..20376)
                     /locus_tag="OO335_000591"
     CDS             complement(19753..20376)
                     /locus_tag="OO335_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6001097.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="OO335_000592"
     CDS             complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="OO335_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR6001098.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="OO335_000593"
     CDS             complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="OO335_000593"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6001099.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21989..23506
                     /gene="dgt"
                     /locus_tag="OO335_000594"
     CDS             21989..23506
                     /gene="dgt"
                     /locus_tag="OO335_000594"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6001100.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23636..25060
                     /gene="degP"
                     /locus_tag="OO335_000595"
     CDS             23636..25060
                     /gene="degP"
                     /locus_tag="OO335_000595"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6001101.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25215..26371
                     /gene="cdaR"
                     /locus_tag="OO335_000596"
                     /pseudo
     CDS             25215..26371
                     /gene="cdaR"
                     /locus_tag="OO335_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26460..26846)
                     /locus_tag="OO335_000597"
     CDS             complement(26460..26846)
                     /locus_tag="OO335_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6001102.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="OO335_000598"
     CDS             complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="OO335_000598"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6001103.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="OO335_000599"
     CDS             complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="OO335_000599"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6001104.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30749..31543)
                     /gene="map"
                     /locus_tag="OO335_000600"
     CDS             complement(30749..31543)
                     /gene="map"
                     /locus_tag="OO335_000600"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6001105.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31911..32636
                     /gene="rpsB"
                     /locus_tag="OO335_000601"
     CDS             31911..32636
                     /gene="rpsB"
                     /locus_tag="OO335_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6001106.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32894..33745
                     /gene="tsf"
                     /locus_tag="OO335_000602"
     CDS             32894..33745
                     /gene="tsf"
                     /locus_tag="OO335_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6001107.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33892..34617
                     /gene="pyrH"
                     /locus_tag="OO335_000603"
     CDS             33892..34617
                     /gene="pyrH"
                     /locus_tag="OO335_000603"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6001108.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34909..35466
                     /gene="frr"
                     /locus_tag="OO335_000604"
     CDS             34909..35466
                     /gene="frr"
                     /locus_tag="OO335_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6001109.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35558..36754
                     /gene="ispC"
                     /locus_tag="OO335_000605"
     CDS             35558..36754
                     /gene="ispC"
                     /locus_tag="OO335_000605"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6001110.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36943..37701
                     /gene="ispU"
                     /locus_tag="OO335_000606"
                     /gene_synonym="uppS"
     CDS             36943..37701
                     /gene="ispU"
                     /locus_tag="OO335_000606"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6001111.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37714..38571
                     /gene="cdsA"
                     /locus_tag="OO335_000607"
     CDS             37714..38571
                     /gene="cdsA"
                     /locus_tag="OO335_000607"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6001112.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38583..39935
                     /gene="rseP"
                     /locus_tag="OO335_000608"
     CDS             38583..39935
                     /gene="rseP"
                     /locus_tag="OO335_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6001113.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39965..42397
                     /gene="bamA"
                     /locus_tag="OO335_000609"
     CDS             39965..42397
                     /gene="bamA"
                     /locus_tag="OO335_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6001114.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42519..43004
                     /gene="skp"
                     /locus_tag="OO335_000610"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42519..43004
                     /gene="skp"
                     /locus_tag="OO335_000610"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6001115.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43008..44033
                     /gene="lpxD"
                     /locus_tag="OO335_000611"
     CDS             43008..44033
                     /gene="lpxD"
                     /locus_tag="OO335_000611"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6001116.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44138..44593
                     /gene="fabZ"
                     /locus_tag="OO335_000612"
     CDS             44138..44593
                     /gene="fabZ"
                     /locus_tag="OO335_000612"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6001117.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44597..45385
                     /gene="lpxA"
                     /locus_tag="OO335_000613"
     CDS             44597..45385
                     /gene="lpxA"
                     /locus_tag="OO335_000613"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6001118.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45385..46533
                     /gene="lpxB"
                     /locus_tag="OO335_000614"
     CDS             45385..46533
                     /gene="lpxB"
                     /locus_tag="OO335_000614"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6001119.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46530..47126
                     /gene="rnhB"
                     /locus_tag="OO335_000615"
     CDS             46530..47126
                     /gene="rnhB"
                     /locus_tag="OO335_000615"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6001120.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47163..50645
                     /gene="dnaE"
                     /locus_tag="OO335_000616"
     CDS             47163..50645
                     /gene="dnaE"
                     /locus_tag="OO335_000616"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6001121.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50658..51617
                     /gene="accA"
                     /locus_tag="OO335_000617"
     CDS             50658..51617
                     /gene="accA"
                     /locus_tag="OO335_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6001122.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51716..53857
                     /gene="ldcC"
                     /locus_tag="OO335_000618"
     CDS             51716..53857
                     /gene="ldcC"
                     /locus_tag="OO335_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6001123.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53914..54303
                     /locus_tag="OO335_000619"
     CDS             53914..54303
                     /locus_tag="OO335_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6001124.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54368..55666
                     /gene="tilS"
                     /locus_tag="OO335_000620"
     CDS             54368..55666
                     /gene="tilS"
                     /locus_tag="OO335_000620"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6001125.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55715..55975)
                     /gene="rof"
                     /locus_tag="OO335_000621"
     CDS             complement(55715..55975)
                     /gene="rof"
                     /locus_tag="OO335_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6001126.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55962..56162)
                     /locus_tag="OO335_000622"
     CDS             complement(55962..56162)
                     /locus_tag="OO335_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6001127.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56328..56873
                     /locus_tag="OO335_000623"
     CDS             56328..56873
                     /locus_tag="OO335_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6001128.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56870..57292
                     /gene="arfB"
                     /locus_tag="OO335_000624"
     CDS             56870..57292
                     /gene="arfB"
                     /locus_tag="OO335_000624"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6001129.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57306..58016
                     /gene="nlpE"
                     /locus_tag="OO335_000625"
                     /gene_synonym="cutF"
     CDS             57306..58016
                     /gene="nlpE"
                     /locus_tag="OO335_000625"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6001130.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58171..58995)
                     /locus_tag="OO335_000626"
     CDS             complement(58171..58995)
                     /locus_tag="OO335_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR6001131.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59048..60766)
                     /gene="proS"
                     /locus_tag="OO335_000627"
     CDS             complement(59048..60766)
                     /gene="proS"
                     /locus_tag="OO335_000627"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6001132.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="OO335_000628"
     CDS             complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="OO335_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6001133.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="OO335_000629"
     CDS             complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="OO335_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6001134.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="OO335_000630"
     CDS             complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="OO335_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6001135.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62958..63611)
                     /gene="metI"
                     /locus_tag="OO335_000631"
     CDS             complement(62958..63611)
                     /gene="metI"
                     /locus_tag="OO335_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6001136.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63604..64635)
                     /gene="metN"
                     /locus_tag="OO335_000632"
     CDS             complement(63604..64635)
                     /gene="metN"
                     /locus_tag="OO335_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6001137.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64823..65398
                     /gene="gmhB"
                     /locus_tag="OO335_000633"
     CDS             64823..65398
                     /gene="gmhB"
                     /locus_tag="OO335_000633"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6001138.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65762..>66032
                     /locus_tag="OO335_000634"
     rRNA            65762..>66032
                     /locus_tag="OO335_000634"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15626 a        16347 c        18196 g        15863 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag
     7921 tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga
     7981 agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct
     8341 actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc
     8461 gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag ccgcgtggtg
     8821 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg
     9421 ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg gtaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
    10081 agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac
    10141 atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag
    10201 atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
    10261 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
    10321 gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
    10381 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
    10441 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
    10501 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta
    10561 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
    10621 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
    10681 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
    10741 ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact
    10801 tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc
    10861 cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc
    10921 gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc
    10981 agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga
    11041 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
    11101 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact
    11161 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
    11221 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
    11281 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
    11341 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
    11401 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
    11461 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
    11521 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
    11581 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
    11641 actctgtgcc actgttcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca
    11701 aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt
    11761 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact
    11821 gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt gatgacaaac
    11881 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
    11941 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
    12001 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
    12061 tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg
    12121 gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga
    12181 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
    12241 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
    12301 cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
    12361 gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt
    12421 atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca
    12481 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
    12541 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct
    12601 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12661 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12721 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12781 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc
    12841 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
    12901 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12961 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc
    13021 agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
    13081 gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
    13141 gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata tcgcccacca
    13201 ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc
    13261 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag
    13321 tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat
    13381 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
    13441 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
    13501 ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga ccccaatcgt
    13561 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
    13621 ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
    13681 gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg
    13741 tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg
    13801 ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc gcgtaaatcg
    13861 ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa
    13921 tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta
    13981 ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc
    14041 caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
    14101 ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt
    14161 tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc
    14221 atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt ttatggtgca
    14281 acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg taaagcgtga
    14341 gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt gtcgctacgg
    14401 cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg caggccgcct
    14461 ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc
    14521 tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag
    14581 tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg
    14641 ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag tttgctgcgc
    14701 tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt
    14761 cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc gcaatcaatc
    14821 agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa
    14881 cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg
    14941 tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg
    15001 cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg
    15061 tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc
    15121 cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt
    15181 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
    15241 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
    15301 tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat cgtgtcgcga
    15361 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg ttgatggctg
    15421 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt
    15481 tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg
    15541 tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc
    15601 cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg ctgtttctgg
    15661 taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg gcggtgacgc
    15721 tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg
    15781 cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg
    15841 acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg
    15901 ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact ccgctgtgcc
    15961 gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta gggatggcgc
    16021 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga
    16081 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
    16141 gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
    16201 tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc
    16261 tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag agccgttaat
    16321 agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca cccgacgtgc
    16381 agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa agcctgcttc
    16441 aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa
    16501 acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa
    16561 gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg cttcttctgc
    16621 cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaatgcc
    16681 cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga
    16741 aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta catcacgacg
    16801 accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc aggttaaatc
    16861 tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac
    16921 ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa
    16981 ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc
    17041 aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca
    17101 ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct ggcctaacgt
    17161 gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc
    17221 aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatcgt
    17281 cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac
    17341 cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc
    17401 ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca
    17461 ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc
    17521 tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac
    17581 accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg
    17641 ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa caagttaaat
    17701 actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt
    17761 tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg
    17821 ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt
    17881 tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc
    17941 gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc
    18001 tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc
    18061 tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc tgttcggcgg
    18121 tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg
    18181 ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat
    18241 tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg ctagggcgcg
    18301 aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat attttccgcc
    18361 tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg
    18421 cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt
    18481 ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta
    18541 tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc
    18601 cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta
    18661 tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata
    18721 ttaccaaatg ggtactgatg gggggggcga ttggcggtct gtgtggattg ctggggtttg
    18781 tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt
    18841 tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc ttactctgct
    18901 tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg
    18961 gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg gaggcgggga
    19021 cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg ccgttaacgg
    19081 ggatcattct ggttctggag atgaccgata actaccagct cattttgcca atgattatta
    19141 ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta tactcggcga
    19201 ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat
    19261 cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg
    19321 gttagaattt gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac
    19381 tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag
    19441 ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt
    19501 atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg
    19561 ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc gttgactaca
    19621 ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg
    19681 gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca
    19741 catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca
    19801 acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc
    19861 cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc
    19921 agttgcgtag atttctgtac gtaaaatcat ggggatttcg cggaccagta catcacgaat
    19981 gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc
    20041 cgcattaaag gctttattca cgctaatgcc gacaaacacc gccagaccaa cggcgtccag
    20101 caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat
    20161 gctggtgacc atggcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg
    20221 atccagcgcc atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc ccagtaccag
    20281 aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccgtaga tggcaaatac
    20341 cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac tgagcacctg
    20401 aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt gctgcgagga
    20461 taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga
    20521 gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa
    20581 tagcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc
    20641 tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttctga atcgactctt
    20701 ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat
    20761 cagcatattg cgctttcaat tgcacgtact gatccagcag ggattgcgcg gcttgttcgg
    20821 ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa
    20881 tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc
    20941 gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt
    21001 ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt
    21061 cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg
    21121 cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg agaaaagaca
    21181 gggcgaccag cgccctgaac agtgatttag ccatgtgcca gtttctgcac cagtgactca
    21241 accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac
    21301 tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg
    21361 cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg
    21421 cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca
    21481 cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg
    21541 tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg
    21601 acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac tttcagcgtt
    21661 ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac
    21721 agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc
    21781 tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg
    21841 ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt
    21901 ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg
    21961 gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa
    22021 ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat
    22081 cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa
    22141 gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat
    22201 ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagccgtc tgaaagagct
    22261 taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa gcattgttga
    22321 gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcagc
    22381 gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc agcctctgac
    22441 tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac cgcttaacga
    22501 gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg
    22561 tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg gtattttaaa
    22621 atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa
    22681 aaagccgggt tattatcttt ctgaagaagc ctatattgcc cgcttgcgta aagaacttaa
    22741 tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg acgacatctc
    22801 ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct
    22861 ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt
    22921 ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca
    22981 gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg cggcacaacg
    23041 atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga
    23101 tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa aacatgtgtt
    23161 tagccatccg gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt
    23221 agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac tggtagaaaa
    23281 agaacgggtg aaacgtttcc ctattgagtc gcgcttattc cacaaactct cgacgcgcca
    23341 tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct
    23401 atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct
    23461 ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag tcttttgtaa
    23521 agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc gggctataaa
    23581 acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa
    23641 aaaaaacaca ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct
    23701 ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc
    23761 accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac
    23821 cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt
    23881 ctgccaggaa ggttctccgt tccagagctc tccgttctgc caggggggcc agggcggtaa
    23941 tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga
    24001 taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca
    24061 gctgagcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat
    24121 cgcgctgatc caaatccaga acccgaaaaa actgaccgca attaagatgg cggattctga
    24181 tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac
    24241 ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga
    24301 aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa
    24361 cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat
    24421 cggtatcggt tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc agatggtgga
    24481 atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga attccgaact
    24541 ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa
    24601 ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa
    24661 gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa
    24721 actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca
    24781 gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat
    24841 gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg gcactccggc
    24901 tgcgcagatc ggcctgaaga aaggtgatgt gattattggt gcgaaccagc aggcagtgaa
    24961 aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat
    25021 tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg
    25081 aatacgggaa ggggttctcc ttacaatctg tgaacttcgc cacaactcca tacatcttca
    25141 tcatcctttg ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc
    25201 ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg
    25261 cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta
    25321 tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt
    25381 cacagggacg agtcgtcgat atcgatgacg cggtagcacg tcatctgcac ggtgtgcggc
    25441 aggggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag
    25501 gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc
    25561 tggaacagtc gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaagaactgg
    25621 tgatgaacct gattcaggca gaggagaata ctcccgcact tctgaatggg cgcaacggct
    25681 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    25741 tggcgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga
    25801 gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    25861 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    25921 ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg
    25981 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    26041 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    26101 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    26161 gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc acaatgtgca
    26221 accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa
    26281 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    26341 tgtggcgtta caactggatg aagagcggta gattatgcgt taaggttgag gcggcgctgg
    26401 ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat
    26461 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    26521 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    26581 cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    26641 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    26701 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    26761 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    26821 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    26881 tattttagca tttggcttgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    26941 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc
    27001 atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga
    27061 tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgagccaga
    27121 aagcatacgg gcagcatcat gctgcccgta tatgtactct taatcgatgg tacgcagcag
    27181 ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca
    27241 gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc
    27301 cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc
    27361 aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt caaccacttc
    27421 agagcgtgcg ccaatgaagc agttatcttc aataatggtc gggttagcct gcagcggctc
    27481 cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca
    27541 agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt
    27601 aacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc
    27661 tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt
    27721 catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat
    27781 acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat
    27841 tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc
    27901 gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa aagcggtttc
    27961 aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc
    28021 tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt
    28081 attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat
    28141 ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg
    28201 cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg
    28261 ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg
    28321 acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac caaaccgaat
    28381 aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc
    28441 catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg
    28501 gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat
    28561 ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc ttaaatcatg
    28621 ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt
    28681 agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag
    28741 tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg
    28801 cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt
    28861 ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag
    28921 atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc
    28981 ctgaatatcg cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc
    29041 aaccagctgt gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg
    29101 agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc
    29161 gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg
    29221 ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc
    29281 atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg
    29341 cggcatatac gcgccgagca cgctatggcg atgcattggc aatagtccgc gccgcaccgc
    29401 tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat tacacagcgg
    29461 ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt
    29521 gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat
    29581 aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgag
    29641 tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag
    29701 catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc
    29761 gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc
    29821 gaataacaga cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca
    29881 cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc
    29941 gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg
    30001 aatatcgcgc aagccgccag ggctactttt gatgtctggt tcaaggttgt agctggtgcc
    30061 atggtaacgc tgatggcgct ggttctgttc ttcaactttc gctgcgtaga acttgtcgga
    30121 aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc
    30181 aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccaa
    30241 catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag
    30301 cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat
    30361 cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcaac
    30421 caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg
    30481 gtctatgaac tcggtacgtg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc
    30541 atcacccagc caacgctgga aagtatcgat atgggctttt atcccacaga cggttaattc
    30601 atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtggaga gagcggtgtt
    30661 tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt
    30721 aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg
    30781 tgtcatcctt gcgtagcgtc aaaatttcgc agccgttatc agtcaccaca atagtatgct
    30841 catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc
    30901 ggatctcttt tttacccgcg ttgaccattg gctcaatggt gaacgtcatc cccggtttca
    30961 gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc
    31021 gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaatt
    31081 tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg cgtagcgcca
    31141 ggtacaggct ttcttgcgtg atgcggcaca ggcgttcgcc catgatggtc ggcttaccga
    31201 cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt
    31261 taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg cacaccactt
    31321 cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg
    31381 cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc
    31441 ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc
    31501 gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc
    31561 gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt
    31621 gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat agaaaattcg
    31681 acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat ttgtggtata
    31741 aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca
    31801 ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa
    31861 tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg
    31921 tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga
    31981 acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg
    32041 agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt gtttctcgca
    32101 aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg
    32161 ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact
    32221 ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg
    32281 gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac
    32341 tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa
    32401 tcgatgctga ccacgaacac attgctatca aagaagcaaa caacttgggt attccggtat
    32461 ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg
    32521 acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag
    32581 gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag
    32641 gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc
    32701 gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg
    32761 aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc
    32821 atatggcccc ctttttcact tttatatctg tgcggtttaa tgccgggcag atcacatctc
    32881 cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac
    32941 tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct
    33001 ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt
    33061 tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt
    33121 taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt
    33181 tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga
    33241 agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc
    33301 tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt
    33361 tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag
    33421 caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca
    33481 ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt
    33541 tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat
    33601 ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt
    33661 catccgtttc gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt
    33721 tgctgcgatg tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt
    33781 ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca
    33841 atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc
    33901 aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc
    33961 actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa
    34021 ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc
    34081 gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg
    34141 accgtaatga acggcttggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt
    34201 ctgatgtccg ctattccatt gaatggcgtg tgcgacagct acagctgggc agaagctatc
    34261 agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt
    34321 accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa
    34381 gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagatccaac cgcaaccatg
    34441 tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg
    34501 gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg
    34561 ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc
    34621 ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt
    34681 ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac
    34741 aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa
    34801 taatccagac gattacccgt aatatgttta atcagggcta tacttagcac acttccactg
    34861 tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat
    34921 atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc
    34981 agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtggaatat
    35041 tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact
    35101 ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcc
    35161 tccgatctcg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg
    35221 ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg
    35281 cgtgttgcag tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa
    35341 gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact
    35401 gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag
    35461 ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc
    35521 gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg
    35581 ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt
    35641 ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga
    35701 attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat
    35761 gctacagcaa caggggagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat
    35821 ggcagcgctt gaggaggttg atcaggtgat ggcagccatt gttggtgctg ctgggctgtt
    35881 acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact
    35941 ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc
    36001 ggtcgatagc gaacataacg ctatttttca gagtttaccg caacctatcc agcataatct
    36061 gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg
    36121 ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc aagcttgccg
    36181 tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa
    36241 aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc agatggaagt
    36301 gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct
    36361 ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa
    36421 tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc
    36481 cgcaccggat tatgagcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg
    36541 ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc
    36601 gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg aaaaaatgga
    36661 tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgagtgaagt
    36721 cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtcgcg
    36781 ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg
    36841 ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag gcttcagtat
    36901 tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa
    36961 ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat tatggacggc
    37021 aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa agccggggca
    37081 aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc gttaacgctg
    37141 tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa
    37201 ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa cgtgcgtctg
    37261 cgtattattg gcgataccag tcgttttaac tcgcgtttgc aagaacgtat tcgtaaatct
    37321 gaagcgctaa cagccggaaa taccggtctg acgctgaata ttgcggcgaa ctacggtgga
    37381 cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg
    37441 caaccagatc agatagatga agagatgtta aaccagcatg tctgtatgca tgaactggcc
    37501 cctgtagatt tagtaattag gactggggga gagcatcgca ttagtaactt tttgctttgg
    37561 caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt cgatgaacaa
    37621 gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag
    37681 cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct gatatctgct
    37741 tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc
    37801 attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct tagcggtttt
    37861 accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt ggcgctgatg
    37921 ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga aatctcactt
    37981 tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta cccaggttcc
    38041 gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct aaccattgtt
    38101 cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa tcattacagt
    38161 ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc cggcgcatat
    38221 atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc gggtaaaacc
    38281 tggcaaggct ttataggtgg actcgctact gcagcggtaa tctcatgggg ttatggcatg
    38341 tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt cgcagcgtta
    38401 gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg aattaaggac
    38461 agcggtcatt taattccagg acacggtggt attttagatc gtattgatag cctgacggct
    38521 gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta acggaaggta
    38581 atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt gtacttatca
    38641 ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt
    38701 tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc accgaatatg
    38761 ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg
    38821 tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa cgagcggcga
    38881 ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac tggctggttt
    38941 ttattattgg tgtgcctggc gtacgtccgg tggttggcga aatagcagcc aattcgatag
    39001 ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc
    39061 ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa agcaccacca
    39121 ttacagtagc gccatttgac agcgaccaac ggcgggatgt aaagctcgat ttacgtcact
    39181 gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt cctcgtgggc
    39241 cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt
    39301 tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag tgggtgacct
    39361 ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc gaaaggcagg
    39421 ggagtccctt gtctttgaca ttaatcccgg agagtaaacc gggtaatggt aaagcgattg
    39481 gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa gttgtacgcc
    39541 agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc
    39601 tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac aacctcagtg
    39661 ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta gtttattacc
    39721 tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt ccgttgcccg
    39781 tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat
    39841 ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg ctgttaatgg
    39901 ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag aacgcataat
    39961 aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg ccaccgtata
    40021 cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt
    40081 tggtgcggcc ctcctcagta tgccggtgcg tacaggcgac acggttaatg atgaagatat
    40141 cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc gcgtccttcg
    40201 tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca gcattacttt
    40261 ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg cttctggtgt
    40321 gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag gtctggaaga
    40381 cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga ccccgctgcc
    40441 gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg aaatccagca
    40501 aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc atttccaact
    40561 gcgtgacgaa gtgccgtggt ggaatgtggt aggcgatcgt aaataccaga aacagaaact
    40621 ggcgggcgat cttgaaaccc tgcgcagcta ctatctggac cgcggttatg cccgtttcaa
    40681 catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa
    40741 catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca accttgccgg
    40801 gcactccgct gaaattgagc aactgactaa gatcgagccg ggcgagctgt ataacggcac
    40861 taaagtgacc aaaatggaag atgacattaa aaagcttctc ggtcgctatg gttatgccta
    40921 tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt
    40981 gaacgttgat gcgggtaacc gtttctacgt gcgtaagatc cgttttgaag gtaacgatac
    41041 ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat ggttggggag
    41101 cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg aaactgtcga
    41161 taccgatacc caacgtgttc cgggtagccc ggaccaggtt gatgtagtct acaaggtgaa
    41221 agaacgtaac actggtagct tcaacttcgg gattggttac ggtactgaaa gtggcgtgag
    41281 cttccaggct ggcgtgcaac aggataactg gttaggtaca ggttatgctg ttggtatcaa
    41341 cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc cgtacttcac
    41401 cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg cagatgacgc
    41461 cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg gcttcccgat
    41521 taacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc tgtccaacat
    41581 gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc cgagcacctc
    41641 tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt ggacctataa
    41701 caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga ccggtaaagt
    41761 gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg cgacttatgt
    41821 gccgatcgat gacgatcaca aatgggttgt tctgggtcgt acccgctggg gttatggtga
    41881 tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg gttccagcac
    41941 cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc cgcatcaggc
    42001 cagtaattat gatccggact atgattacga atgtgcgact caggacggcg cgaaagacct
    42061 gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc tcgagttcat
    42121 caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt cattcttctg
    42181 ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg gatatccgga
    42241 ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc
    42301 attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg gagacaaggc
    42361 agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag
    42421 tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac gcaaagaact
    42481 gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg ttattagctg
    42541 caggtcttgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca
    42601 atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac acgctggaaa
    42661 atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg caggctaaaa
    42721 tgaaaaaact gcagtccatg aaagcgggca gcgatcgcac caaactggaa aaagacgtga
    42781 tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc
    42841 gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg aaatccgttg
    42901 ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac aacagcagcg
    42961 atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg ccttcaattc
    43021 gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc gatatcgtca
    43081 tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc atggttaacc
    43141 caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg
    43201 atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg acttacgcgc
    43261 gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca cccagtgcgg
    43321 tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac gcggtgattg
    43381 agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa
    43441 acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac catgagatcc
    43501 agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac ggctttggtt
    43561 atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta attattggcg
    43621 atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat gacactgtta
    43681 ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc gtgattggcg
    43741 acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact
    43801 gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg
    43861 ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat tcctcaggca
    43921 ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg aacattgatg
    43981 acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac taacgttcca
    44041 tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca ggccgtgtta
    44101 ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca ctcatactct
    44161 gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac tggtggatcg
    44221 cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga
    44281 gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc tgattctgga
    44341 agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac tggaaccggg
    44401 tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg
    44461 cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga cccgttttaa
    44521 aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag
    44581 ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg ccattgtgga
    44641 agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg ttggacccca
    44701 tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa
    44761 aattggccgc gataatgaga tttatcagtt tgcctccatc ggcgaagtta accaggattt
    44821 gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag
    44881 cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg gcagcgacaa
    44941 cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc gctgtattct
    45001 cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga tcatcggcgg
    45061 catgaccgca gtccatcagt tctgcatcat tggtgcgcat gtgatggttg gcggctgctc
    45121 cggtgtggcg caggacgtcc ctccttatgt tattgcgcag ggtaaccacg caacgccgtt
    45181 cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga ttaccgctat
    45241 ccgcaatgcg tataagctaa tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga
    45301 aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt tctttgcacg
    45361 ctcaacgcgc ggtctgattc gttaatgact gaccagcgtc cattaacgat tgccctggtc
    45421 gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct gaaagaacgt
    45481 gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa
    45541 gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct cggtcgtctg
    45601 cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact gaagccagat
    45661 gtttttgttg gtattgatgc gcccgacttc aatattactc ttgaaggtaa cctcaaaaag
    45721 cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc
    45781 gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg
    45841 ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc tgatgccatg
    45901 ccgttagatc cagataaaaa tggtgcccgt gatgtgctgg ggatccctta cgatgcccac
    45961 tgtctggcat tgttgccggg cagccgtggt gcagaagtcg aaatgcttag tgccgatttc
    46021 ttgaaaactg cccagctttt gcgccagaca tatccggatc tcgaaatcgt ggtgccgctg
    46081 gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctg
    46141 tcggttcatt tgctggatgg aatgggccgt gaggcgatgg tcgccagcga tgcggcacta
    46201 ctggcgtcgg ggacggcagc cctggagtgt atgctggcga aatgcccgat ggtggtggga
    46261 tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac tgattatgtc
    46321 tcgctgccaa atctgctggc gggcagagag ttagtcaaag agttattgca ggaagagtgt
    46381 gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac
    46441 gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc cgatgagcag
    46501 gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg cacacgcagc
    46561 tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg
    46621 cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa aagctgagcg
    46681 aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc tggagtctgg
    46741 gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc atgctggcga
    46801 tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt gatggtaacc
    46861 gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggggatagc cgcgtaccag
    46921 aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg
    46981 atattgtttt cccgcaatat ggttttgccc aacataaagg gtatccaacc gcttttcatc
    47041 tggaaaaact ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt gggcctgtca
    47101 aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac cggaatctga
    47161 agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg atgatcgatg
    47221 gcttggccaa aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg ccagcactgg
    47281 cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg ggacatggcg
    47341 cagggattaa gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg ctgggtgatg
    47401 agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat ctggcgttgc
    47461 tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact
    47521 ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg ggcgacgtcg
    47581 gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag
    47641 aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg gatgaagaaa
    47701 gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacta
    47761 acgatgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc
    47821 acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg cagcaatata
    47881 tgcgtagcga agaggagatg tgtgagctgt ttgccgacat cccagaagcc cttgccaaca
    47941 ccgttgagat cgccaaacgc tgtaacgtaa ctgtgcgtct tggtgaatac ttcctgccgc
    48001 agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca aaagaaggcc
    48061 tggaagagcg tctggcattt ttattccctg acgaggaaga acgtcttaaa cgccgcccgg
    48121 aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc ttcccgggct
    48181 acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta ccggtagggc
    48241 caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc accgacctcg
    48301 atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt gtctccatgc
    48361 ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgaa cacgtggcgg
    48421 acatgtacgg tcgtgatgcg gtatcgcaga ttatcacctt cggtacgatg gcggcgaaag
    48481 cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc gaccgtatct
    48541 cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa gccgagccgc
    48601 agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac atggcgcgca
    48661 aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt atcgcgccga
    48721 ccaaaattac cgattttgcg ccgctttatt gcgatgaaga gggcaaacat ccggtcaccc
    48781 agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc cttggtttgc
    48841 gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg
    48901 gcgagccacc gctggatatc gccgcgatcc cgctggatga caagaaaagc ttcgacatgc
    48961 tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg aaggacctga
    49021 tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca ctgttccgcc
    49081 ctggtccgtt gcagtcaggg atggtggata actttatcga ccgtaaacat ggtcgtgaag
    49141 agatctccta tccggacgtg cagtggcagc atgaaagcct gaaatcggta ctggagccaa
    49201 cctacggcat tatcttgtat caggaacagg tcatgcagat tgcccaggtg ctttctggtt
    49261 ataccctcgg tggcgcggat atgctgcgcc gtgcgatggg taagaaaaag ccggaagaga
    49321 tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac
    49381 tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt aacaaatcgc
    49441 actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg cactatccgg
    49501 cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag gtggtgggcc
    49561 tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata aactccggtc
    49621 tttaccattt ccacgtcaac gacgatggcg aaatcgtgta tggtattggc gccatcaaag
    49681 gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc ggctacttcc
    49741 gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg
    49801 aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga
    49861 actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa gctatcggtc
    49921 aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa tcctacgcca
    49981 gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg ttaggcctgt
    50041 acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat gtcggaggcg
    50101 taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct gcggggctcg
    50161 ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg
    50221 atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat aaataccagc
    50281 aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt gatgacttca
    50341 gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat
    50401 atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag cttttaaacc
    50461 gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat ctctactatc
    50521 agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg
    50581 atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa ctggagtttg
    50641 actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg attgcagagc
    50701 tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa ctggatatta
    50761 acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg cgtaaaatct
    50821 tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccgcag cgtccttata
    50881 ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc gaccgtgcgt
    50941 atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga
    51001 tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc
    51061 cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa cgctttaaga
    51121 tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc gcagaagagc
    51181 gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt ggcgtaccgg
    51241 tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg
    51301 ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa ggttgtgcgt
    51361 ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg
    51421 ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg ctgggcggcg
    51481 ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg gcggatctgg
    51541 ccgatctcga tgtgttaagc actgaagatt taaaaaatcg tcgttatcag cgcctgatga
    51601 gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt ggcccttttt
    51661 tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg aacacatgaa
    51721 catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca tcaaagaact
    51781 ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca gcgttgattt
    51841 gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact gggatgagta
    51901 cagtctcgat ttatgcagcg atatcaatca acttaatgaa tatctcccgc tttatgcctt
    51961 catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg cgctctggtt
    52021 ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc agtacaccaa
    52081 cgaatatctt gataacatta cgccgccgtt cacgaaagcc ttgtttacct acgtcaaaga
    52141 gcggaagtac accttttgta cgccggggca tatgggcggc accgcatatc aaaaaagccc
    52201 ggttggctgt ctgttttatg attttttcgg cgggaatacc cttaaggctg atgtctctat
    52261 ttcggtcacc gagcttggtt cgttgcttga ccacaccggg ccacacctgg aagcggaaga
    52321 gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg gaatatcgac
    52381 gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt tgatcgaccg
    52441 caactgccat aaatcactgg cgcatctgtt gatgatgaac gatgtagtgc cagtctggct
    52501 gaaaccgacg cgtaatgcgt tggggattct gggggggatc ccgcgccgtg aatttactcg
    52561 cgacagcatc gaagagaaag tcgctgccac tacgcaagca caatggccgg ttcatgcggt
    52621 gatcaccaac tccacctatg atggtttgct ctacaacacc gactggatca aacagacgct
    52681 ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tatactcatt ttcatccaat
    52741 ctaccagggt aaaagtggta tgagcggcga gcgtgttgcg ggaaaagttt tcttcgaaac
    52801 gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcactgatcc acattaaagg
    52861 cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca cctcgcccag
    52921 ttatcccatt gttgcttcgg ttaagacggc ggcggcgatg ctgcgtggta atccgggcaa
    52981 acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg tccagcggct
    53041 gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg tggatgaagc
    53101 cgaatgcagg cccgttgcgc ctggcgaaca atggcacggc tttaacgatg cggatgccga
    53161 tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg acgaacaggg
    53221 caatatgagt gaggagggga tcccggcggc gctggtagca aaattcctcg acgaacgtgg
    53281 gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta ttggcatcga
    53341 taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaagcgct cttacgatct
    53401 caacctgcgg atcaaaaata tgctgcccga tctctatgct gaagatcccg atttctaccg
    53461 caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta aacacgatct
    53521 ttccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga cgccacatca
    53581 ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgctggaac aactggtcgg
    53641 tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt tgatgccagg
    53701 agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc tttgttccgt
    53761 agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg acgaagacgg
    53821 cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg cttccgggcg
    53881 agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca ggttcaccat
    53941 attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga tattcttggt
    54001 ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg
    54061 cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga gcgccccagc
    54121 cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat cgatgcggca
    54181 gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga tccatacacg
    54241 caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact gtatgagcag
    54301 taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac tgtaaaatcg
    54361 caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga ttctggtggc
    54421 ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt ggcggacgga
    54481 aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg ccaatgccga
    54541 tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc tggtggtcga
    54601 acgcgtacaa cttgcgcaag aggggctggg gattgaggcc caggcgcggc aggcacgtta
    54661 tcaggcattt gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc aacatctcga
    54721 cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg ccgggctttc
    54781 ggctatggcg gaagtttcgg agtttgctgg aacacggctt atccgcccgt tgcttgcccg
    54841 cacgcggggg gaactggagc agtgggcatt ggcgcatggt ttacgctgga ttgaagacga
    54901 aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag tgccgttatt
    54961 gcagcagcgt tggccgcatt ttgcggaagc aacggcccgc agcgccacac tttgtgctga
    55021 acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc aaacgtcgca
    55081 ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgtcgcg cggcgattat
    55141 tcgccgctgg ctggcagggc agaatgcacc gatgccttcc cgcgacgcgc tggtgcgcat
    55201 ctggcaggaa gtggcgctgg cgcgggaaga tgcttccccc tgtttacgtt tgggcgcctt
    55261 tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg ggcaaagcga
    55321 aaccattgtg ctgtggcaga cgtggctaca accgctggaa ttaccggcgg ggctgggaac
    55381 cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag cggtcagcgt
    55441 gcgtttcaaa gcgccaggat tgctgcatat tgtcgggcgc aacggcggac gtaagctgaa
    55501 gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc cactgctgtt
    55561 ttatggagaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag gtgtggctga
    55621 aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga aagccggata
    55681 agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ttcgcttacc accaccgtac
    55741 cgatttcagg gtggctaaag ctggtaattt tatccagacg cagctcgcgg gtttcgccag
    55801 cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcactg gcttttgcct
    55861 gcaatttttc gccatctttc agctcaagtg tcagcattaa atgatgctgg caggcgagct
    55921 caagattatc gtaatcatca caattaattg gttgatacgt atcattcatt gacataatcg
    55981 ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc agaaagggcg
    56041 tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat
    56101 cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca atatttttcc
    56161 acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc ctacgaagat
    56221 aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag cgccgcaact
    56281 gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag
    56341 cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg
    56401 cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg ctgcgcttgc
    56461 tggcgtggct gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg tgtgccgatg
    56521 atgagccgga agcgtggctg cgtaacgatc acctgggcat tgatttgtgg attgagctgg
    56581 ggctgccgga tgagcgacga attaagaaag cctgcaccca ggccgcagaa gtggcgctgt
    56641 ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc aaatgtgtgc
    56701 agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa gtaagcgcct
    56761 ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggtgttatc tggttatcgg
    56821 atgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca tgattgtgat
    56881 ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc gtgcgcaggg
    56941 cgctggcggg cagcatgtta ataagacctc aacggctatt catctgcgtt ttgacattcg
    57001 ggcgtccagc ctgccagagt attacaaaga gcgtctgctc gccgccagcc atcatttgat
    57061 cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg aactgaaccg
    57121 cgaagccgct ctggcccggc tggtggcagt gattaaagat ttaacaaaag aacaaaaagc
    57181 ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat cgaaagcaca
    57241 aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat aaaaagaagg
    57301 aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc tttactctga
    57361 tgggatgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct gccgaactga
    57421 aaccgatgcc gcaaagttgg cgcggcgtgc taccgtgtgc cgattgcgaa ggaatcgaaa
    57481 tctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat ctcggtgctc
    57541 gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct gacaagctgg
    57601 tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat gcgctggaga
    57661 tgctcgatcg tgaaggcaat ccaattgaat cgcagttcaa ctatacgctg gaaccggcac
    57721 aatccagttt acctatgacg ccgatgaccc tgcgaggcat gtatttttat atggctgatg
    57781 cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc
    57841 tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa accggtgtta ctgtcagtag
    57901 aaggtcactt tacgcttgag gctaatccgg atactggtgc gccaaccaaa gtattagcgc
    57961 ccgatacggc gggtaaattt tacccgaata aggattgcag tagtttgggg cagtaaacct
    58021 gcttaaggca gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag gcctacagca
    58081 tataatgcaa cattttgaat tagcacgatt ttgtaggccg gataaggcgt ttacgccgca
    58141 tccggcattt tgtgcacgag gctggcttta tcactgagtc agcccaacaa agcgacccgc
    58201 cttgatataa atcccaggct tcaggtgccc aacatactgt aactgcgttt ctggcttaaa
    58261 cgcagagaca tcaccaatac gcatatgcat cagatcggca gggctaatcc agcccgattg
    58321 cgccagtgta agtggatgcc cggatttggc aaatgcatca gtgacaaatt ccgaacaaaa
    58381 ccacgacttt ttgtctcctt cgcacacact gcttaactgc gctttcgcca ggtcgctgac
    58441 gcactgttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat
    58501 aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc
    58561 aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag
    58621 cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc
    58681 ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa
    58741 gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt
    58801 gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa
    58861 tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct
    58921 gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaagcaggc ggcagtacgc
    58981 ctttggttta tccattttat acaatccatg taaaaaaggg ccctgaaatt caggaccctt
    59041 tctggcatca gcctttaatc tgtttcacca gatattcgac gatgtcacca gtcttaatta
    59101 actgtttctc gccgttgcga cgatatttat attcgatatc gtcgttgtcg aggttacggt
    59161 cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac atcacgcccg
    59221 gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcacgcagt tcgctgtaca
    59281 gtttctcagc aagctcttgt acgcggaagg atttgtgcat gttcatcggc agaatcgcca
    59341 cctggaacgg cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag ttctgctcaa
    59401 tcgccgcagc taccacacgc gttaccccga taccgtagca acccatcgtc aggatttggt
    59461 tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg gtacccagct
    59521 ggaagatgtg accaacttcg ataccacgtt tgatcagcag cgtaccctgg ccatccgggc
    59581 ttggatcgcc agccaccacg ttacggatat ctgcaacttc cggggtagcg acatcgcgat
    59641 cccagttgat accgaagtag tgtttaccat cgatgttagc accagcagcg aaatcactca
    59701 tcgccgcaac ggtacggtca atcaccaccg gaatcggcat gtttaccgga cccagtgaac
    59761 ccggaccggc tttaaccaca gcacgaattt cttcttcggt cgcgaaagtc agcgggcttg
    59821 caacctgcgg cagtttttct gctttaactt cgttcagctc gtgatcaccg cgcaccagca
    59881 gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac cagcagagtc ttaaccgttt
    59941 tctcaatcgg cagattgaac tgttcaacca gttccgcgat ggttttcgcg ttcggcgtat
    60001 caaccagcgt catttcctgg gtagcagcag cgcgcggttc tttcggcgcg atagcttctg
    60061 ccagttcaat gttcgctgca tagtcagagg tgtcggagaa gaccacatcg tcttcaccgc
    60121 tctgcgccag cacctggaat tcgtgagagg cgctgccgcc gatagaaccg gtgtcggcct
    60181 gtacggcgcg gaaatccagc cccatgcggc tgaagatttt gctgtaggcc gcatacattg
    60241 catcgtaggt ttcctgcagg gattcctgag aagtatggaa agagtaagca tctttcatca
    60301 ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg aacttggtct
    60361 ggatctgata gaagttcagc ggcagctgtt tgtaagagct aagctcgtta cgaatcaggt
    60421 cagtgataac ttcttcatga gttgggccga gtacgaacgg acgctcgcca cggtcaacaa
    60481 aacgcagcag ttccggaccg tactgttccc aacgaccact ctcttgccac aaatcggctg
    60541 gctgaaccac cggcatcgac acctcgatcg caccggcgtt gttcatctct tcacgcacga
    60601 tgttttcgac ttttttcaga acgcgcacgc cggtcggcag ccaggtatat aacccggagg
    60661 ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg gctgatcacc tcggcgtcgg
    60721 caggtgtctc cttgagagtg gagagcaggt attggctagt acgcatgttg ttacggttcc
    60781 agttggaagg tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat ttagtttacc
    60841 agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac
    60901 ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata
    60961 ggttttgccc gtttcctcac ctttacgata ggccgggcgc gggtcctgcg ccagtacttc
    61021 gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac
    61081 ctctgcggta aaactcaccg ccatctctgc agctggcgca ctttgcgcat agctggcact
    61141 ggcatcggga agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc
    61201 atcgaccaga tccagactgc cgagcttcag aatcacgctg cctttatggc aaacaacctc
    61261 tttcagctct accagcgaca tgccaattgg gttagggcgg aaagtagagc gtgttgcgaa
    61321 aacccccatt ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc
    61381 ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt ccaggccgcg
    61441 aacggcgtcg gcctggttgt aggaagctat gagatgcagt tcgccgttgg cgctttttac
    61501 cagacctggc tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat
    61561 ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca
    61621 gcctgacgat agcagcccgg cgtaccgctg gtgacttcgc agctatgcag taatacagca
    61681 ttggctttca ttttagaggc attgatttgc atctgcttac gtgcggttgg aatgctcggc
    61741 ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac
    61801 ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct
    61861 ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg
    61921 gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca
    61981 cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa
    62041 atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa
    62101 aattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca
    62161 tcagactgat aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc
    62221 gttacgatca ggtttacgta tggggagtct ttatcttcga caaagatacc gtctttcgcc
    62281 ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg
    62341 tcgtccagag aacgcggcag ttgcggtgct tccagttcaa caattttcag attttttggg
    62401 ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag
    62461 cccacttttt gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca
    62521 acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc
    62581 ggataaacaa aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga
    62641 tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg
    62701 tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc
    62761 gcaactttct gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta
    62821 atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg
    62881 gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat
    62941 ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcaaac
    63001 tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgttg
    63061 tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca
    63121 cccatcgcgg aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc
    63181 ggcaacgctt ccggtaacag cactttacag acgatctgca ttggcgtggc acccattgcg
    63241 cgggaagctt caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca
    63301 ataaacggtg ctgcaccaac ggttaacgga acaattgctg cctgcaaacc aatcgatgta
    63361 ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa
    63421 cggaaaatgt tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc
    63481 cccggacgcg tgacataaag cagaacgcca accggcagac caatcacaaa cccaaaaaat
    63541 ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcag ccacatcatc
    63601 ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc
    63661 ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga atttaacgcc
    63721 accggcgtag tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt
    63781 ttcagaaagc agtggggcat cgaccgattg accggtaaac tccagacgca gcatcggcac
    63841 gcagtcagta aatggctccg tttgcagacg ttcctggtaa tcttccggga tatccagatg
    63901 cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact
    63961 taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg
    64021 cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat
    64081 gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc
    64141 atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt
    64201 ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga
    64261 cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg
    64321 cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat
    64381 accaatctgg cggcgagctt tggtcaactc ggattctgac agcgtggtca attcctggcc
    64441 atcgaccagc acgctaccct cggttgggcg ctccagcaag tttacacaac gtataagcgt
    64501 actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag
    64561 gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt
    64621 cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtagc atctcgaatg
    64681 cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa
    64741 tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg
    64801 ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga
    64861 tggcaccatt aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga
    64921 cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac
    64981 caaccagtct ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga
    65041 gtggatggac tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc
    65101 gcatcatccg caggggagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca
    65161 tccggggatg cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat
    65221 ggtgggcgat aaattagaag atatgcaggc agctgttgcg gcgaacgtgg gaacaaaagt
    65281 gctggtgcgt acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt
    65341 aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cgcaatgatt
    65401 aaaagatgaa cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc
    65461 tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg
    65521 ttgaagagaa aaaatcctga aattcagggt tgactctgaa ataggaaagt gtgatatacg
    65581 ccacctcgcg acagtgagct gaaagctgcg tcgcaactgc tctttaacaa tttatcagac
    65641 aatctgtgtg ggcactcgaa gatacggatt cttaacgtcg caagacgata aatgaatacc
    65701 aagtctcaag agtgaacacg taattcatta cgaagtttaa ttctttgagc atcaaacttt
    65761 taaattgaag agtttgatca tggctcagat tgaacgctgg cggcaggcct aacacatgca
    65821 agtcgaacgg taacaggaag cgcttgctgc ttcgctgacg agtggcggac gggtgagtaa
    65881 tgtctgggaa actgcctgat ggagggggat aactactgga aacggtagct aataccgcat
    65941 aacgtcgcaa gaccaaagag ggggaccttc gggcctcttg ccatcggatg tgcccagatg
    66001 ggattagctt gttggtgggg taacggctca cc
//
LOCUS       DAJTSF010000009        64196 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000009 DAJTSF010000000
VERSION     DAJTSF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64196
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.009"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            131..574
                     /locus_tag="OO335_000635"
     CDS             131..574
                     /locus_tag="OO335_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6001139.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            810..1628
                     /gene="ybhA"
                     /locus_tag="OO335_000636"
     CDS             810..1628
                     /gene="ybhA"
                     /locus_tag="OO335_000636"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR6001140.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1629..2687)
                     /gene="modC"
                     /locus_tag="OO335_000637"
     CDS             complement(1629..2687)
                     /gene="modC"
                     /locus_tag="OO335_000637"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6001141.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2690..3379)
                     /gene="modB"
                     /locus_tag="OO335_000638"
     CDS             complement(2690..3379)
                     /gene="modB"
                     /locus_tag="OO335_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6001142.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3379..4152)
                     /gene="modA"
                     /locus_tag="OO335_000639"
     CDS             complement(3379..4152)
                     /gene="modA"
                     /locus_tag="OO335_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6001143.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="OO335_000640"
     CDS             complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="OO335_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6001144.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4597..5385
                     /gene="modE"
                     /locus_tag="OO335_000641"
     CDS             4597..5385
                     /gene="modE"
                     /locus_tag="OO335_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6001145.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5453..6925
                     /gene="modF"
                     /locus_tag="OO335_000642"
     CDS             5453..6925
                     /gene="modF"
                     /locus_tag="OO335_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6001146.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7186..8202
                     /gene="galE"
                     /locus_tag="OO335_000643"
     CDS             7186..8202
                     /gene="galE"
                     /locus_tag="OO335_000643"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6001147.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8212..9258
                     /gene="galT"
                     /locus_tag="OO335_000644"
     CDS             8212..9258
                     /gene="galT"
                     /locus_tag="OO335_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6001148.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9262..10410
                     /gene="galK"
                     /locus_tag="OO335_000645"
     CDS             9262..10410
                     /gene="galK"
                     /locus_tag="OO335_000645"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6001149.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10404..11444
                     /gene="galM"
                     /locus_tag="OO335_000646"
     CDS             10404..11444
                     /gene="galM"
                     /locus_tag="OO335_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6001150.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11647..12399
                     /gene="gpmA"
                     /locus_tag="OO335_000647"
     CDS             11647..12399
                     /gene="gpmA"
                     /locus_tag="OO335_000647"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6001151.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="OO335_000648"
     CDS             complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="OO335_000648"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6001152.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13934..14314
                     /locus_tag="OO335_000649"
     CDS             13934..14314
                     /locus_tag="OO335_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6001153.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14428..15369
                     /gene="zitB"
                     /locus_tag="OO335_000650"
     CDS             14428..15369
                     /gene="zitB"
                     /locus_tag="OO335_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6001154.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="OO335_000651"
     CDS             complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="OO335_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6001155.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="OO335_000652"
     CDS             complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="OO335_000652"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6001156.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17415..17490)
                     /locus_tag="OO335_000653"
     tRNA            complement(17415..17490)
                     /locus_tag="OO335_000653"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17455..17457),aa:Lys,seq:ttt)
     gene            complement(17494..17569)
                     /locus_tag="OO335_000654"
     tRNA            complement(17494..17569)
                     /locus_tag="OO335_000654"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17534..17536),aa:Val,seq:tac)
     gene            complement(17621..17696)
                     /locus_tag="OO335_000655"
     tRNA            complement(17621..17696)
                     /locus_tag="OO335_000655"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17661..17663),aa:Lys,seq:ttt)
     gene            complement(17699..17774)
                     /locus_tag="OO335_000656"
     tRNA            complement(17699..17774)
                     /locus_tag="OO335_000656"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17739..17741),aa:Val,seq:tac)
     gene            complement(17910..17985)
                     /locus_tag="OO335_000657"
     tRNA            complement(17910..17985)
                     /locus_tag="OO335_000657"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17950..17952),aa:Lys,seq:ttt)
     gene            complement(18150..18941)
                     /gene="cpoB"
                     /locus_tag="OO335_000658"
     CDS             complement(18150..18941)
                     /gene="cpoB"
                     /locus_tag="OO335_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6001157.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18951..19472)
                     /gene="pal"
                     /locus_tag="OO335_000659"
     CDS             complement(18951..19472)
                     /gene="pal"
                     /locus_tag="OO335_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6001158.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19507..20799)
                     /gene="tolB"
                     /locus_tag="OO335_000660"
     CDS             complement(19507..20799)
                     /gene="tolB"
                     /locus_tag="OO335_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6001159.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20932..22173)
                     /gene="tolA"
                     /locus_tag="OO335_000661"
     CDS             complement(20932..22173)
                     /gene="tolA"
                     /locus_tag="OO335_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6001160.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22238..22666)
                     /gene="tolR"
                     /locus_tag="OO335_000662"
     CDS             complement(22238..22666)
                     /gene="tolR"
                     /locus_tag="OO335_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6001161.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22670..23362)
                     /gene="tolQ"
                     /locus_tag="OO335_000663"
     CDS             complement(22670..23362)
                     /gene="tolQ"
                     /locus_tag="OO335_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6001162.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23359..23763)
                     /gene="ybgC"
                     /locus_tag="OO335_000664"
     CDS             complement(23359..23763)
                     /gene="ybgC"
                     /locus_tag="OO335_000664"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6001163.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23913..24206)
                     /gene="ybgE"
                     /locus_tag="OO335_000665"
     CDS             complement(23913..24206)
                     /gene="ybgE"
                     /locus_tag="OO335_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6001164.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24206..24319)
                     /gene="cydX"
                     /locus_tag="OO335_000666"
     CDS             complement(24206..24319)
                     /gene="cydX"
                     /locus_tag="OO335_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6001165.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24334..25473)
                     /gene="cydB"
                     /locus_tag="OO335_000667"
     CDS             complement(24334..25473)
                     /gene="cydB"
                     /locus_tag="OO335_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6001166.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25489..27057)
                     /gene="cydA"
                     /locus_tag="OO335_000668"
     CDS             complement(25489..27057)
                     /gene="cydA"
                     /locus_tag="OO335_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6001167.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27906..30542)
                     /gene="mngB"
                     /locus_tag="OO335_000669"
     CDS             complement(27906..30542)
                     /gene="mngB"
                     /locus_tag="OO335_000669"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR6001168.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVI
                     RYEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPT
                     SFNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAV
                     FSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLS
                     CRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMP
                     PVGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEEN
                     QGSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30560..32476)
                     /gene="mngA"
                     /locus_tag="OO335_000670"
     CDS             complement(30560..32476)
                     /gene="mngA"
                     /locus_tag="OO335_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6001169.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32646..33368
                     /gene="mngR"
                     /locus_tag="OO335_000671"
     CDS             32646..33368
                     /gene="mngR"
                     /locus_tag="OO335_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6001170.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33471..34340)
                     /gene="sucD"
                     /locus_tag="OO335_000672"
     CDS             complement(33471..34340)
                     /gene="sucD"
                     /locus_tag="OO335_000672"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6001171.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34340..35506)
                     /gene="sucC"
                     /locus_tag="OO335_000673"
     CDS             complement(34340..35506)
                     /gene="sucC"
                     /locus_tag="OO335_000673"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6001172.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35600..36817)
                     /gene="odhB"
                     /locus_tag="OO335_000674"
     CDS             complement(35600..36817)
                     /gene="odhB"
                     /locus_tag="OO335_000674"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6001173.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36832..39633)
                     /gene="sucA"
                     /locus_tag="OO335_000675"
     CDS             complement(36832..39633)
                     /gene="sucA"
                     /locus_tag="OO335_000675"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6001174.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39934..40650)
                     /gene="sdhB"
                     /locus_tag="OO335_000676"
     CDS             complement(39934..40650)
                     /gene="sdhB"
                     /locus_tag="OO335_000676"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6001175.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40666..42432)
                     /gene="sdhA"
                     /locus_tag="OO335_000677"
     CDS             complement(40666..42432)
                     /gene="sdhA"
                     /locus_tag="OO335_000677"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6001176.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42432..42779)
                     /gene="sdhD"
                     /locus_tag="OO335_000678"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42432..42779)
                     /gene="sdhD"
                     /locus_tag="OO335_000678"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6001177.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42773..43177)
                     /gene="sdhC"
                     /locus_tag="OO335_000679"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42773..43177)
                     /gene="sdhC"
                     /locus_tag="OO335_000679"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6001178.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43558..43665
                     /locus_tag="OO335_000680"
     CDS             43558..43665
                     /locus_tag="OO335_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001179.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43871..45154
                     /gene="gltA"
                     /locus_tag="OO335_000681"
     CDS             43871..45154
                     /gene="gltA"
                     /locus_tag="OO335_000681"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6001180.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45545..46111
                     /locus_tag="OO335_000682"
     CDS             45545..46111
                     /locus_tag="OO335_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6001181.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46162..48621
                     /locus_tag="OO335_000683"
     CDS             46162..48621
                     /locus_tag="OO335_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6001182.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48636..49367
                     /locus_tag="OO335_000684"
     CDS             48636..49367
                     /locus_tag="OO335_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6001183.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49364..50440
                     /locus_tag="OO335_000685"
                     /pseudo
     CDS             49364..50440
                     /locus_tag="OO335_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50592..51638
                     /locus_tag="OO335_000686"
     CDS             50592..51638
                     /locus_tag="OO335_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6001184.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51635..52426)
                     /gene="nei"
                     /locus_tag="OO335_000687"
     CDS             complement(51635..52426)
                     /gene="nei"
                     /locus_tag="OO335_000687"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6001185.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52462..53196)
                     /gene="pxpA"
                     /locus_tag="OO335_000688"
     CDS             complement(52462..53196)
                     /gene="pxpA"
                     /locus_tag="OO335_000688"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6001186.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53186..54118)
                     /gene="pxpC"
                     /locus_tag="OO335_000689"
                     /pseudo
     CDS             complement(53186..54118)
                     /gene="pxpC"
                     /locus_tag="OO335_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54112..54768)
                     /gene="pxpB"
                     /locus_tag="OO335_000690"
     CDS             complement(54112..54768)
                     /gene="pxpB"
                     /locus_tag="OO335_000690"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6001187.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54791..55534)
                     /gene="ybgI"
                     /locus_tag="OO335_000691"
     CDS             complement(54791..55534)
                     /gene="ybgI"
                     /locus_tag="OO335_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6001188.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55805..57286
                     /gene="dtpD"
                     /locus_tag="OO335_000692"
     CDS             55805..57286
                     /gene="dtpD"
                     /locus_tag="OO335_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6001189.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57436..58854)
                     /gene="phrB"
                     /locus_tag="OO335_000693"
     CDS             complement(57436..58854)
                     /gene="phrB"
                     /locus_tag="OO335_000693"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6001190.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58851..59360)
                     /locus_tag="OO335_000694"
     CDS             complement(58851..59360)
                     /locus_tag="OO335_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6001191.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59489..59776
                     /locus_tag="OO335_000695"
     CDS             59489..59776
                     /locus_tag="OO335_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR6001192.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59831..60211)
                     /locus_tag="OO335_000696"
     CDS             complement(59831..60211)
                     /locus_tag="OO335_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001193.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60214..63600)
                     /locus_tag="OO335_000697"
     CDS             complement(60214..63600)
                     /locus_tag="OO335_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6001194.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PPPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(63619..63822)
                     /locus_tag="OO335_000698"
                     /pseudo
     CDS             complement(63619..63822)
                     /locus_tag="OO335_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15694 a        17277 c        15622 g        15603 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg
      601 ccacttgtaa ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg
      661 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      721 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      781 aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag
      841 acggcacctt attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc
      901 gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc
      961 atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt
     1021 tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc
     1081 tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg
     1141 caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc
     1201 tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac
     1261 aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt
     1321 ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg
     1381 ttgatattgc acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc
     1441 aaggttggtc gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc
     1501 tggaagccgc tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg
     1561 ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc
     1621 tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt
     1681 ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg
     1741 acttccagct ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc
     1801 aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc
     1861 tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc
     1921 tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact
     1981 ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac
     2041 acttcctcca gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct
     2101 gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc
     2161 cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc
     2221 agcggttcat ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg
     2281 cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct
     2341 aaaagcgcca ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga
     2401 ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga
     2461 cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg
     2521 acaatccgcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt
     2581 ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg
     2641 gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg
     2701 cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg
     2761 caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg
     2821 tatacatggc agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg
     2881 ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg
     2941 gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc
     3001 ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca
     3061 tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg
     3121 taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta
     3181 ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga
     3241 gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg
     3301 gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt
     3361 ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc
     3421 ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac
     3481 aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt
     3541 tacccctttg ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg
     3601 ttcgaccagc gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt
     3661 atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc
     3721 cggatcgcca accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc
     3781 gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag
     3841 cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt
     3901 ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt
     3961 agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc
     4021 aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc
     4081 tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca
     4141 tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa
     4201 ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg
     4261 caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc
     4321 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     4381 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     4441 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     4501 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     4561 tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc
     4621 cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt
     4681 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     4741 tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca
     4801 ggcggtaaag gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc
     4861 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     4921 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     4981 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat
     5041 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5101 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5161 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     5221 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     5281 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     5341 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     5401 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     5461 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     5521 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc
     5581 ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     5641 gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg
     5701 gcaacggaat aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc
     5761 tgagattatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     5821 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     5881 aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     5941 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6001 gtccggtatt actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca
     6061 gtttgctggc gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct
     6121 ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc
     6181 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct
     6241 gaacaatggc gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt
     6301 gaatccaggc gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt
     6361 aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg
     6421 acgtcgcggc agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag
     6481 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     6541 ttttgattcg attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca
     6601 gtggctggat attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc
     6661 ctggggacag cagcgtttgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     6721 tattctcgat gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt
     6781 tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga
     6841 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     6901 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     6961 cgcactctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7021 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7081 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7141 gttatgctat ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt
     7201 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     7261 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     7321 cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
     7381 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     7441 gtcgccgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     7501 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     7561 accgtttatg gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg
     7621 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa
     7681 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     7741 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     7801 caagttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     7861 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     7921 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     7981 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8041 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8101 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8161 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     8221 tttaatcccg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     8281 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     8341 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     8401 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     8461 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc
     8521 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     8581 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     8641 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     8701 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gtgcgaagac
     8761 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     8821 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     8881 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     8941 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9001 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9061 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9121 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9181 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
     9241 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
     9301 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
     9361 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
     9421 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     9481 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
     9541 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
     9601 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     9661 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     9721 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
     9781 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
     9841 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
     9901 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     9961 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10021 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10081 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
    10141 atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10201 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    10261 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    10321 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    10381 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    10441 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    10501 ggggtgcgac tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    10561 tgctcggctg tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta
    10621 ttggtcgtta tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga
    10681 cgctttcgcc aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    10741 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    10801 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    10861 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    10921 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    10981 agattctggc ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga
    11041 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11101 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11161 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    11221 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    11281 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    11341 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    11401 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    11461 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    11521 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    11581 tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    11641 aagcatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    11701 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    11761 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    11821 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    11881 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    11941 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    12001 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12061 cgttacgcga aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt
    12121 gaccgcgtga tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc
    12181 gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg
    12241 agcgaagaag agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc
    12301 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg
    12361 aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg
    12421 agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat
    12481 tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc
    12541 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt
    12601 gacgtaacag agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt
    12661 aggccagcgg ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca
    12721 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    12781 ccatctgctt tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag
    12841 ctggcagacc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    12901 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa
    12961 tcgccgaatg cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    13021 tggcatcgat agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag
    13081 aaagaccaga cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc
    13141 cccagctcat caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg
    13201 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    13261 tgatctggaa gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg
    13321 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    13381 gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg
    13441 ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    13501 gggcatgggc aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga
    13561 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    13621 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    13681 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    13741 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    13801 tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca
    13861 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    13921 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    13981 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    14041 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    14101 taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg
    14161 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    14221 agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    14281 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    14341 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    14401 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    14461 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    14521 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    14581 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    14641 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    14701 tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    14761 cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg
    14821 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    14881 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    14941 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    15001 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    15061 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    15121 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    15181 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    15241 atccaacact acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat
    15301 caaccttgtc ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    15361 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    15421 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    15481 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    15541 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    15601 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    15661 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    15721 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    15781 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa
    15841 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    15901 aaaataatgc caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc
    15961 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    16021 agatcataac cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    16081 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    16141 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac
    16201 atgaacctcg tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt
    16261 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg
    16321 tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc
    16381 ggttgccaca ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt
    16441 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    16501 ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg
    16561 cacaatgcag gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg
    16621 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    16681 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga
    16741 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc
    16801 ttcaacaggg cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt
    16861 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    16921 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    16981 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    17041 cgcattacgt tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat
    17101 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    17161 gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc
    17221 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    17281 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    17341 tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg
    17401 gcgctttttt gcattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
    17461 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
    17521 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
    17581 ggatactaca aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc
    17641 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg
    17701 gtgggtgatg acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc
    17761 tgagctaatc acccgacact acgctggata ctacaactaa ctatgttagt gggggtcgtg
    17821 caggatgact cgacttcgtc tcgcccttcg gcgccgttgc tgatgcaacg ttatccttca
    17881 cgttttacta ccgcttccac cttagcgatt ggtgggtcgt gcaggattcg aacctgcgac
    17941 caattgatta aaagtcaact gctctaccaa ctgagctaac gacccacttt ttcgttgctt
    18001 tcggtttgtt tgatatcccg tggcaacggc ggcatatatt actgatttca gacttgagcg
    18061 caacaaaaat ttcgatgcag atcactcaac tgcttatgat tcgcacgaca cgaccagaaa
    18121 taatgcgact tctggtcgtg tgttatgcat tacatcgcgt tcagacgttt ttgtgcctgt
    18181 ttagcgccat cagtaccagg gtatttactg ataacctgct ggtatacggc tttcgctttt
    18241 gcggtgtcac ctttgtcctg catgatgacg ccgactttaa acatcgcatc agcagccttt
    18301 ggtgacttcg gatagttttt cactaccgaa gcaaaatagt acgccgcatc atctttttta
    18361 cccttgttgt agtttaactg acccagccaa taattggcgt ttggcaggta agttgaatca
    18421 gggtaatttt tgatgaaatt ctgaaatgcc accattgcgt catcctggcg ggatttatcc
    18481 tgcaccagcg caatagctgc attgtaatcc gtgtttgcat cgccgctttt caccggcgcg
    18541 ccagcattcg cagtaccagc atcagctgtc ggcgtcgttg atgccgtcgc accgctttga
    18601 tcgctgctgg ttgattgcgc cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg
    18661 atctgctttt gccgctccac gacctgattc agttgatact gattttcctg aatctgacca
    18721 cgcagggaat caatatcgga ttgattatca gaaagttgtt gctggagttg ggttaaaagc
    18781 tggctgtgag cattagaaat acgctcaagt tgagtgacgc ggtcttcgac cgagcctgag
    18841 ctgacactac tgattggtgc ctgagcaaaa gcggcccagg gggccgctat accaaccagt
    18901 aacgacagac tcaatagttg atgtctgaag ttactgctca tgcaattctc ttagtaaacc
    18961 agtaccgcac gacggttttt ggagtatgcc gcttcgtcat gacccagtac tgcaggtttt
    19021 tctttaccgt aagaaacgat ggagatctgg tctgcagaaa cgcctttacc ctgcaggtac
    19081 atcttaacgg cgttcgcacg acgttcaccc agggagatgt tgtattccgg agtaccacgt
    19141 tcgtccgcgt gaccttctac ggtgactttg taagacgggt tgctacgcag gaagtttgca
    19201 tgtgcatcca gcatttgagc gaagtcagaa cggatatcgt acttgtccag atcgaagtaa
    19261 acgatgttgt tctgctgcag ctgttgcatt tgcagacgag cctgctcttc ggaagacatg
    19321 ttgccgttgc cgccgttcgc atccatacca gtgccggcac ccagcatgcc ttcgctgccg
    19381 tcattgctgg cgttcttgtt ggaagaacat gccgcaattg ccataacagg cagagcaatc
    19441 atcagccctt tcagcacttt gttcagttgc atttcaatga ttcctttact attcaattaa
    19501 ttattatcac agatacggcg accaggcagg gaatttgacc tgtccatcag ttgccggaag
    19561 acgcgctttg aaacgcccat ctgtagaaac caaattcagc acggatccca tcccctgaga
    19621 agagctgtag attaccatag tgccgttagg tgccagactt ggcgtttcat ccaggaacgt
    19681 ggacgacaga acttgtacgc ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc
    19741 accattggag ctgaccatta ccataaattt accgtcgctg ctgacatccg catcctggtt
    19801 ctgcgaacct tcccaggtaa tacgttgtgg cgcaccgccg ttgatattca ctttataaac
    19861 ctgcggacga ccggcctggt cagaagtaaa tgccaggttc tggctatccg ggaaccaggt
    19921 cggttcggta ttgttactgc gaccatcagt cacctggcgg atctgaccag aagccaaatc
    19981 cattacgtac aggttcagac taccggtttt cgacaaggcg aatgccagtt tgctgccgtc
    20041 tggcgagaat gcaggtgcac cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc
    20101 atttgccagc gtctgaataa ccagcgcgga acgaccgctt tcgaaggtca cataagccag
    20161 tttagaaccg tctggtgacc acgccggaga catcagcggc tgcggtgaac ggtgaacgac
    20221 aaactggttg taaccgtcat agtcagatac gcgcagttca tacgggaact gaccgccgtt
    20281 ggtctgaaca acgtaggcaa tacgggtacg gaacgcacct ttaatgccgg tcagtttttc
    20341 aaacacttca tcactggcgg tatgaccagc ataacgcagc cactgcttgt tcactttgta
    20401 cgagttctga gcaagtacag tacccggtgc gccgccagtg tcaacaagtt gataagcaac
    20461 attgtaagaa ccatccggat tcggagtgac ttgaccgaca actacagcgt caatgcccag
    20521 tgcggaccat gcagctggtt gtacttcctg cgcactaccc ggctgctgtg gcagacgagc
    20581 gcgatctaac ggattaaatt taccgctgtt acgcaagtca gcagcaacga tgccgccaat
    20641 atcttcaggt gccgcaccag gccccgccca ctggaaagga acaacaccaa taggacgacc
    20701 ggaatctaca ccgctgtcga tcacaatgcg gacttcagca tgcagaactg atgcccacag
    20761 tatgagaaaa ccaaatgcta ctcgtaatgc ctgcttcatc atatctccct tatctggacc
    20821 gatggcccac gataatttag cagaatgtta acaaactaaa atacacaaaa ctaccagaac
    20881 cccgtggcaa ccggtgcctg atgttgaccg tccgaacagt caacatcgcg attacggttt
    20941 gaagtccaat ggcgcgtttt tgaacacttc atatactgcc tggcttggtg gtttcgggat
    21001 cttcgcaagt ttagctgctg ccaacgcagc ctgacaaagt gcgggatcgc caccttcagg
    21061 tttgatatcc agtaacatac catcgggtgc cagttttatg cgcagcgtac aggttttgcc
    21121 tgcataggac gatgcgtcat agaacttact ttcgatagca gatttaatct gcccggcata
    21181 gttattgata tcggcccctg atgcgccatt gtttttagta ttaccactcc cggcaggcga
    21241 agcattgttc cctttcgccc ctccccccgt tttcggtgca ttcttaccag agcttagctc
    21301 accgaaaata tcatctgcct ctgcggcagc ttttgctgca gcggcttttt cagctgctgc
    21361 ttttgccgct gccgcttttt tgtctgcggc ggctttttct gccgctgctt tcttatcagc
    21421 tgctgcctta tcagcagccg ctttttctgc tgcttttttg tcggctgcag ctttctctgc
    21481 tgccgctttc ttatcagctg cagccttttc agcagccgct ttcttctcag cttctgcctt
    21541 ggctttttca gcagcttcag ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc
    21601 cgcttccgct ttcttcttca gtgccgcagc ggctacctcg gcttttttct gcgcttcggc
    21661 tgcggctttg gcggcttctg cttctgcttt tttcttcgcg tctgcagccg ctttcttcgc
    21721 tgcttcttcc gcagctttag catctgccgc cgctttcgct gccgccactt cagcttgctt
    21781 ctgctttaac tcggcctgtt ttgcggcttc ttcagcctgc tttttctgct cctgagccgc
    21841 taaccgctct ttctcaagtt gcttcaggcg ttcctgttca gccgcttgtt tctcacgcag
    21901 ttcttcagca gcctgctgtt ccttcatctt gcgctgttca tcagaacgct tcgcgcttga
    21961 ttcctggctt tgcatgcgtt tgtactgctc aactaccgca cctgaatcaa ccatgacagc
    22021 gtcgatggac gaaccaccgc cgcctccggc tgaagcttct atattctcat cgaacgaact
    22081 ccagatcagc gccgcaaata agatgacatg cagcactgct gaaataatta tcgcccgctt
    22141 gagcttgtcg ttttgttcgg ttgcctttga cactctcggt ttccaaaaac tgttcgcctg
    22201 ttacccgctc tctttcaagc aagggaaacg cagatgttta gataggctgc gtcattaaac
    22261 caaccgattt cacacccgca ctatgtaaca agttcagtgc tttaattatt tcatcgtaag
    22321 gcacatcttt tgcgccaccg atcagaaaga ccgttttcgg gttggccttg aaacggctgg
    22381 acacttccgc caccacctgc tctggtggta aacgctccag gcgatctttc tcaaccacca
    22441 cggtgtactg accaatacca gacacttcaa caatcactgg cggattatcg ttactgctca
    22501 ccgcctgtga ttcagtagcg tctggcagat cgacctccac gctctgggtg atgatgggcg
    22561 ctgtcgccat aaagatcagc aacagcacca gcagtacgtc cagcaacgga acaatgttga
    22621 tttcggactt gagatcgcga cgacctcgtc cacgcgctct ggccatggct taccccttgt
    22681 tgctctcgct aacggtaaac gcctggcggt gcagaatcgc ggtaaactct tccataaagt
    22741 tgtcgtaatt cagttccagt ttgtttacgc gctggttgag acggttataa gccattaccg
    22801 ccggaattgc ggcgaacaga ccaattgccg tcgcaatcaa cgcttctgcg ataccgggcg
    22861 caaccatttg cagtgttgct tgttttaccg ccccgagggc gataaaggcg tgcatgatcc
    22921 cccagaccgt accaaacaga ccaatatacg ggctgatgga gccaaccgta ccgaggaacg
    22981 gaatgtgcgt ttccagattt tcaagttcac ggttcatgga gatacgcata gcacgcgacg
    23041 ccccttccac tacggcttcc ggcgcatggc tattggcacg atgcaggcgc acaaactctt
    23101 tgaacccgct gtaaaagatt tgttccgaac cagtcagatt atcccgtttc ccctgactct
    23161 cttgatagag gcgagagagt tcgattccag accagaattt atcttcaaac gcttcggctt
    23221 cacgcgccgc tgcgttaaga atacgggttc gctggataat aatggcccag gatgcgattg
    23281 aaaaaccaat caaaatcaac atgataagtt taaccagaag gctagccttc aggaacaaat
    23341 caaggatatt catgtcagtc actgcttaaa ctccgcgaca atagacttgg gaagcgcacg
    23401 aggcttcatt ttgagtgggt caacgcaaac aaccagaacc tctgcttcat tcagcaaagt
    23461 attctcggcg ttgacaatac gttgcgtgaa aaccaaagag gtgccacgca ttgatgttat
    23521 ttcagtctgt atttcgagca tatcgtcgag ccgcgcaggt gcgtaatatt ccaccgtcat
    23581 tttacgtacc acaaaggcaa cgcgttcagc catcagcgcc tgctgactga agtgatgatg
    23641 acgcagcatc tctgtgcgtg ctctttcata aaaagcgacg taactggcgt ggtacaccac
    23701 accaccggca tcggtatctt catagtagac gcgaaccggc catcgaaaca gcgttgtatt
    23761 cactttacat cccggtaatg caacaaaagt tagagctttt aaacgctgct actatacgcg
    23821 cgcgattgag gtttgggaat gagggcaagt taagggcgcg taaaaattta tgggcctaga
    23881 gcggcccata attttttaaa gagaattctg atttaaaaga agaagaaaat cagcccgaca
    23941 atgagaacaa tatcggcaag caatgggcaa aaaatccctt gccaaagaac cttctgcgga
    24001 cgaaagccca cgccgtgaat cacaccagca cagacggccc acatcagcaa taaaccatgc
    24061 cagatttcca gttcactggt cttcgcggca aaacgagatg ggtcccaaaa catacatcct
    24121 gctaacagaa gcgccatcac gaaggaaagc gcccgcaggg ggcgcttgtc cattaccgca
    24181 tacaaggtcg cgataatctt actcatcaga tgtcttcttg accggctttg cctgattcga
    24241 cgtgttcaag cgccagcgcg gtgattaccc caaacgaaca ggcaagaagc gttcccagaa
    24301 tccatgcgaa ataccacatt tttagctcct tacttagtac agagagtggg tgttacgttc
    24361 aatatcttct ttggtgatac gaccgaacat tttccagtaa caccaggcgg tgtagagcag
    24421 aatgatcggt accagaacca ccgcaaccca ggtcatgacg ttaagcgtca gctggctgga
    24481 agttgcatcc cacattgtca gacttgcgtt catcatggtg ctggacggca tcacaaacgg
    24541 gaacattgcg ataccggctg tcaggatgat gcaggccagc gtcagggagg agaacacaaa
    24601 cgcccacgcg gctttatcca tacgtgcagt caggatggtc agcagcagca gaaccacacc
    24661 cagtgccgga atagcccaca gaattggcgt gttgttgaag ttaaccagcc atgcgccagc
    24721 ttcacgaacc acttctttat tcagtgggtt agaggctgcg taatggtcca ttgtcgattt
    24781 cacgacataa ccatcgatac cgtacatcac ccatacgcca gccagtgcga aacagaccag
    24841 tgtcaccagc gcagccacct gagccgttgc acgggtacgc aggtgcagtt cgcccacggt
    24901 acgcatttgc agataggttg cgccctgagt aatgatcatc cctacgctca ccacgcctgc
    24961 cagcaggccg aacgggttaa gcaactggaa gaagttaccg gtgtagtaca gacgcagata
    25021 ttcatcaacg ttgaacggta cgccctgcaa caggttaccg aacgctacac caattaccag
    25081 cggcggaacg aagctaccaa tgaagatgcc ccagtcccac atgttacgcc agcgggtttc
    25141 ttcaatcttg gagcggtagt caaaaccgac cggacggaag aacaaagacg ccagcacgag
    25201 gatcatcgcc acatagaagc cggagaacgc agcggcatag accatcggcc aggcagcaaa
    25261 gagtgcgccg cccgcggtga tcagccaaac ctggttaccg tcccagtgtg gtgcaatgga
    25321 gttaatcata attcgacgct cggtgtcgtt acgaccgagg aaacgggtga gcatgcccac
    25381 ccccatgtcg aaaccgtcag tgactgcaaa accaatcagc agaacgccaa ccagcagcca
    25441 ccagataaaa cgcaatactt cataatcgat catttgacga ctcctgtctt agcgtgccgg
    25501 ctgagtagtc gtggaagact gctcaaagtg atagcgaccg gttttcaggc tgcttgggcc
    25561 gaggcgtgca aacttgaaca ttaagaacaa ttctgccacc aggaacaggg tatacaggcc
    25621 gcaaatcagc accattgaga agatgagatc gcctgcggtc agtgacgaat tcgccacagc
    25681 tgtcggcagc acttcaccga tagcccacgg ttggcggcca tattcagcca cgaaccagcc
    25741 cgcttctaca gcaatccacg gcagcggaat accgtacagc gcggcgcgca gaagccattt
    25801 tttctcgcca atgcggttgc ggatgacact ccagaaagag agcgcgatga ttgccagcag
    25861 caggaagcca cacgccacca tgatacggaa cgcaaagtac agcggcgcta cacgcgggat
    25921 ggagtctttg gttgcctgtt gaatctgcgc ttcagtcgca tcagccacgt ttggcgtata
    25981 gcgtttcagc agcagaccgt aaccgaggtc tttcttcatg ctattgaact ggtcacgaac
    26041 cgtctggtcg gtagaaccag aacgcagttg ttcgagcaga gagtacgcct tcatcccgtt
    26101 acgaatgcgt tcttcatgct gcaccatcag ctctttcagg ccgataaccg gggtatccac
    26161 ggaacgcgtt gcaatgatgc ccagtgcgta agggatctga atcgcaaatt tgttcgtctc
    26221 ctcttcctga tcaggaatgc cgaacaaagt aaaggcagca ggcgcaggtt gcgtttccca
    26281 ctcggcttca atagcagcca gtttggtttt ctgcacgtcg cccatttcgt agccggattc
    26341 atcacccaga acaataacag acagaacagc agccataccg aagctggcag cgatagcaaa
    26401 ggagcgttta gcgaaggcga agtcacgacc tttcagcata taccatgcgc tgataccgag
    26461 gatgaacatc gcgccagtca cataaccaga cgctacagtg tgaacgaatt tcacctgagc
    26521 aaccgggtta agcaccagct cggagaagct caccatctcc atacgcatag tttcaaagtt
    26581 gaaatcggac gcgattgggt tttgcatcca gccgttcgca accagaatcc acagtgcgga
    26641 caggtttgaa ccgagcgcca ccagccaggt gacacacata tgctgaactt tacccagacg
    26701 atcccaaccg aagaagaaca gacctacaaa ggtggattcg aggaagaagg ccatcagacc
    26761 ttcgattgcc agcggcgcac cgaagatatc ccctacatag tgggaatagt aagaccagtt
    26821 agtcccgaac tggaactcca tggtcagacc ggtagccaca cccagagcga agttgatacc
    26881 aaacaacttg ccccagaact tggtcatatc tttataaatc tgtttgccgg agaggacgta
    26941 gaccgtttcc ataatggcca ggaggaacgc cataccgagc gtcagtggca caaaaaggaa
    27001 gtggtacatc gcggtcaagg caaactgtaa gcgcgacagt tcgactatat ctaacatcat
    27061 gactccttgc tcatcgcatg aagactccga gagtgaaccc cgttagaaag ggtcacacgc
    27121 atgccccaat agaaatttgt ttgaacactt tcatcatacc atgtgaatgc acacaaaaac
    27181 ggcgtaaaga aaggttgcaa acatgttaat aaaaactcaa attgatccca cgtatatatt
    27241 acgccgcaaa atccttacaa taaacaggtt tttgttgaag caaatttgca tttttcgacg
    27301 gtgatcaatt tatagctaaa ttgccgcctt tcagccaatt tgatcgagaa caatttatct
    27361 ctttttgatg cccatttcca agacttatac attgataaat atcaatttct acaaattatg
    27421 ttaatcatgt ttcaacaagg taactttagt gatatttatg tttaaaatgt gtgaattata
    27481 agactgaaat tagtttcggt tggcaattaa agataaaaag agacgaagca aaaacagcac
    27541 ggttgacctt aattcttttt ttggtcactg gctaatacga cgcagagttt caggattatt
    27601 aataaaggca taaatctccc tcccgccgat attaacattt aattaactta taattatatt
    27661 ataattaact cacccgtggt tattattttg ttaaaaataa agccacatat acgtagcttg
    27721 agagtgaaga taaacacaaa attacctgat gcgctacgct tgccagtgtc tacatcatct
    27781 ctgcaatata ttgaatttgc ctgcttttgt agaccgaata aagcgttcat gccgtatccg
    27841 gcatgaacaa agcgcccttt gtcaacaatc tggccgcgca tatgtggctt cggtttgatt
    27901 gctattcagg caagccggta actgaacgtc cgtgactggc cgggtaaaaa aggccccgat
    27961 agatttgaac cttgattttc ctcggtggta atgtgttcat ccatcatcgt ttctgagcaa
    28021 gaaatcacct cgcgactgaa agcaacagtc gcatcacagg ttgctgattc agccggatta
    28081 aacagccgta aaatcacttc ttgtcggtct tcagctttct taagtgcgct tatcaggcat
    28141 cccactgggg gcattttcaa caaactataa ctttccggca cgttgaatcc ggctttgttg
    28201 agcttcatcg catcccatgg gattttgttg taacactgta ctggagtcag ccatgctcgc
    28261 gcctgctgag ctacaccagc ggcggttggc gtaccggtat aactcaataa acttaggcga
    28321 caagaaagca gaccacgtag ttgtgagtcc gggactggca ttttaattcc cgaaggccgc
    28381 ctaggcctta aaagcagatc ttctttcccc agtaagccca cgccgcgcag caacgtaatg
    28441 gcaaaggttt tcttctcttc accgatgact tcaaattcac gtagcccttc gctaaagaca
    28501 gccatgccgt tacgcccttc ctgtaaggct gcatagttga gcatattcca taccggaacc
    28561 ggcgcttctt tccagctttc ttgctgccag ttgttcattg cactgtcgtt cacggggcgc
    28621 gttagcgaac caaactgggt atctgccaga acactatcgg tgttaaaaga tgtagggatc
    28681 aggacgcgaa ggcgatgatc gtcagcctgg ttatcaaggt tgatatccac atcaatgcgc
    28741 ctgttgttat gactaagagt gacaaccaac tcgacatcta ctctgccagt ggattgtctt
    28801 gcgctgcgtt gttgcagatt acgcggcacc gccatttcat agcggataac agccctgctc
    28861 tgccaggcct catgaataat ctcgcattgc ggtttcgcat tcgccgaagt aattacccac
    28921 tcttcttttg cgggtgaata gtcatattca tcaccatcat cagagctttc ttcaataatt
    28981 tgcaacaccc ggtcatagcg cacaccgcta tctttatcta ccagttgtag cgttccatcc
    29041 tcattgagcg caatttgcca gaaagcattt tccagtatcc cttcagcgtc actttttgcc
    29101 gcaatcacgt tgccaggctg attcgcttcg atataaagcg tgcgatagcc catagaaggg
    29161 acaatctggt tgatctgtat atcaaactcc ataaagggat cgtaattacc gtaatgaact
    29221 atttgccgat cgattaggcc tggatcgatc tcacgggcat ggcgaataaa atacggtaca
    29281 ggctgaccgc gatcgtctcg tagattaaac tggctggcgc gcagccgcac agtggtgttg
    29341 atcacttctt cccgcggcca gggcatcagg ttaaacagga cgagtttgtc ggcgtcgctc
    29401 tgcggcatgt tgtcggcgat tttacgcata tagaaacgca gcagattatc cgccatgtct
    29461 tcagccagtt cgaagcgggc gacgatttcg cgatgaactt tgtcacgaca gcagcagccg
    29521 atgctgtcgt gggcatgatt ttttaagatc tctttccaca ttttctccag caagccgtgg
    29581 tggtatccaa aacccaacgt cctggccagc gttgccagcg gttccagcag attaacaatc
    29641 ttattttcaa tacgtgcgtg ggcaactttg atatccatgc gcgtggaacc gatggtgcga
    29701 tgcacgcgca tatatttgcc atcaataaat tcccctttca gggttgccag attatctcgc
    29761 tgcgcttcga tcttctcaaa tacctcttca aagcggctca tcacaaactt tcgttgagga
    29821 taaatttcgc gcagtttctc catcacttcg aagatatttt gctgcaatgg catctgatca
    29881 tgcccattcg gcagcaaaat ctctttggtt acagacgctt tttccagcac gtcaaaataa
    29941 ctgtcgaggc gtttgcgtaa tccgttttcg tccgcaggta agtacttacc gatggcataa
    30001 cccaacggca tcacctgcgc cgtcacttcg ctaccgtcac tgctttgcca caaaaactcg
    30061 gttttatccg taccgtggcg ctcagaacat ccacgccaga acatggtgca ggtaatgcca
    30121 aatccattgt agatatgcgg cagttgcccg gacatgccaa aggaatcggg taaataacct
    30181 attttcatcg gctcgccaaa cgcgaggcag tcacgcattc cgtacatcag attacggacg
    30241 atggattccg cagaaacaat cgtggtatcg gtctgggtat accaggggcc gataatcaac
    30301 ttgccggctt ctaccagttt cttcaagcgg tctttgtttt ccggtttcac cgcgaaataa
    30361 tcttcgagga tcgccgtttg cccgtcgagt acgtaatatt tgtattcgtt gtcctgttcc
    30421 agtcggcaca ggatctcttc catattattg accagcagaa tgcgtgactc ttcggtggtg
    30481 aaataccact cccgatccca gtgcatatgc ggggtgatgt gaacgcgaga taccgctttc
    30541 atcgtcgttt cctttttgtt tatggcatta cgccatcagt cagatagttg ccatgcttaa
    30601 ccgcgtgacg ccgccagatc aggagaattg cagtcgagat tgcagccccc accaatgccg
    30661 caccaaacca gccaattgcc gccataacac cgcccgcgcc attatcatga agtaaaaaga
    30721 gcgagaaaat gccagcaccg ggtgtcgaaa ggccgatatt catcgcaccg acaatagcgc
    30781 ccgttaccat agagcccagc acaaacgaac caataacccg cagcggatct tcaatcgcca
    30841 tcgggatcgc cccttcggta atacctgcca gccctaacag ccaggtggat ttcccggtct
    30901 caatttcaaa ctctttaaac aggcgcggtg cgagcatcgt ggaagcggtt acggtaaatg
    30961 ccgaaaccat tttgacggag gcgaaaatgg catacgggcc gtaaacgccg ttcgccattg
    31021 cgcccaggca gaatgcataa gcggctttat tcactggccc tccaaggtca aaggaacaca
    31081 taaaaccgag aatggcaccc agcaacagcg cgttacttcc tgacagaccg ttcagccagg
    31141 cggtaagcga gttattgatc caggcgacag gttcccccac cacaaacagc atcagactgc
    31201 ccgctcccaa tgtaccgagc accgggtaga gataaaaagt caggaagcca ttgaatttac
    31261 tgctaagacg caagtgattt ttcatccagc gtatcaagta accggctatc aatccgccaa
    31321 cgaccgcgcc gagaaacccg gagccgatca tgttggcggc aagtccagcc gcaaagcctg
    31381 gcgctaacgc cggtttatct gccagcgaat aggcggtata ggctgccagc accggtacca
    31441 tcaaaattcc gagcagcccg ccgcccagtt tgcggtacat ccacagccag gagttttctt
    31501 cattaaacag atcttgtagc ccgaagattt gcgacagtaa taccgcgacc gccagcaccg
    31561 tcccccccgc gacaatcaac ggtacggcaa aagagatccc gctcaacagt gcctgtttca
    31621 gctccgtttt gacgcttttc accggttgcg tatcttgctg taaggtacgc gtctcatcgc
    31681 cacgctcgag ggtaagcgat tgttggatca acgcttctgc atggcgaatc ggctcggcaa
    31741 caggcacaga aagcgcagga atgccgttaa aacgctcact ctccttgatg gcgacttcag
    31801 ccgcaaaaat acaggcagtt gcactattga gttgatccgc cgttaaacgc ccttcaatac
    31861 cgttagcacc ttgcttttca acgtagacat tcacgccgag tttgcgtccg gctttttcca
    31921 gatactccgc agccatgtag gtgtgagcaa tacccgccgg gcaggccgtt acgcaaacga
    31981 tagttggcgc gttggaaaaa gaggcagaag gttgcgtgcc tcccttgtca tccagcgccg
    32041 agagcaattc atcaggcgtc gtcgccgact gtatacgcgc ccgaatctca tcatccgcca
    32101 ggcgcgtggt cagcgctgtc agcagttgca tgtgcgtagt acccgcttca ttgaggggaa
    32161 tcgccagcaa caccactaaa tcaaccgctt ccgggccatc cacgccttcc cactgaagcg
    32221 gctcgctgag tgtggcgacc gcaaacgccg cttcttttac cgcagcagtt ttgccatgcg
    32281 gcacagccaa cccttcactt aaggcggtcg ggccaaggct ttcacggcga tacacttctt
    32341 tcagaaattg ctcagtactg gaaattttcc ccagagcagc aagacgttga gtcaacgcgt
    32401 gaatggcctc ttcacggctg gtaaagcgcg cattcagaca caacgcatcg cggtgggtca
    32461 gagtcgtcag gttcatcatg atcctcactt gatcgttttt atagtcgcgc cagtgtgccc
    32521 gacaaaacaa taccattgcg taatcaattt cacttgattg aaattatttg cattttattt
    32581 gtattgttta caggatgagt ttatcgggca taatggctaa attcgtatta atgagatgct
    32641 aaataatggg acacaaaccc ttataccgac agattgctga tcgcattcgt gagcaaattg
    32701 cgcgtggcga gcttaaacct ggcgatgcgt tacctaccga atcggcatta caaacagagt
    32761 ttggcgtcag ccgggttacg gtgcgtcagg ctttgcgcca gttagtcgag caacagatcc
    32821 tcgaaagcat tcagggcagc gggacttacg tcaaagaaga gcgggtcaat tacgatattt
    32881 ttcagttaac cagttttgat gaaaaactgt cggaccgtca cgtcgataca cacagtgaag
    32941 ttctgatatt tgaagtgatt ccggctgacg attttcttca gcaacagcta caaatcaccg
    33001 tgcaggatcg tgtttggcat gtgaagcgcg tgcgctatcg caagcaaaag ccaatggcgc
    33061 tggaagaaac ctggatgccg cttgcgttgt tcccggatct cacctggcag gtcatggaaa
    33121 attcgaaata tcactttatt gaagaagtga agaagatggt tatcgatcgt agcgaacagg
    33181 aaatcattcc tctgatgcca accgaagaga tgagccgtct gcttaatatc agccagacaa
    33241 agccgattct tgaaaaagta tcgcgtggat atttagttga tggtcgggtg tttgaatata
    33301 gccgcaacgc ttttaatacc gatgactata aatttaccct gatagcgcag cgaaaatcat
    33361 cgcgataagc acaaaaaggc catcatacga tggccaacca tgtcgaaacc gacagccctt
    33421 cgccattcct gtagagggaa gcggcgaggg ctatttctta ttacagatat ttatttcaga
    33481 acagttttca gtgcttcacc gatatccgcc aggctgcgaa cggttttcac gcctgcggct
    33541 tccagagcag cgaatttctc atccgcagtc cctttcccac cggcaatgat cgcacccgcg
    33601 tggcccatac gtttgccttt cggcgcagtc acaccagcga tgtaacccac aactggcttg
    33661 gtaacatgct ctttgatgta cgctgccgct tcttcttcag cgctaccgcc gatctcaccg
    33721 atcatcacga tcgcttcggt ctgcggatct ttttcgaaca tttcgagaat gtcgataaag
    33781 ttagagccag ggatcgggtc accgccgata ccgacacagg tcgactgacc gaaaccgtaa
    33841 tccgtggtct gtttaaccgc ttcataggtc agtgtaccgg aacgggaaac gatacccact
    33901 ttacccggtt tgtgaatgtg acccggctgg ataccaattt tgcattcacc cggggtgata
    33961 acgcctgggc agttcgggcc gatcatacga acgcctgctt cgtccagttt cactttcacg
    34021 gtcagcatat ccagcgtcgg gatgccttca gtgatggtga taatcagttt gatgcctgca
    34081 tcgatagctt ccagaatgga gtctttgcag aacggtgctg gtacgtaaat aacagacgcg
    34141 gaagcgccag tagcagcaac agcttcacgc acggtgttga acaccggcag gccgaggtgg
    34201 gtagtgccgc ctttacctgg agttacgcca ccaaccattt tagtgccgta tgcaatggcc
    34261 tgttctgagt ggaaagtccc ctggctaccg gtaaagccct ggcagataac cttggtgttt
    34321 ttatcaatta aaatggacat tatttcccct ccactgcggc aacaacctgc tgagctgcat
    34381 ccgtcagacc ttttgctgca ataatattca ggccgctgtc agccagtttc ttcgcgccga
    34441 gttcggcgtt gttaccttcc agacgtacca cgaccggtac gttaacaccc acttccgcta
    34501 ccgcgccgat gataccgtca gcgatcaggt cgcaacgaac gataccgccg aagatgttaa
    34561 ccagaacggc tttcactttg tcgtcagaga ggatgatttt gaacgcttcg gttacacgtt
    34621 ctttggttgc gccgccgcca acgtccagga agttagccgg ttcgccgccg tgcagtttaa
    34681 ctatgtccat cgtacccatc gccagacctg cgccgttaac catacaaccg atgttaccgt
    34741 ccagcgcaac gtagttcagt tcccactgtg cagcctgtgc ttcacgcgga tcttcctgcg
    34801 actggtcacg catttcgcgc agatcaggct ggcggaacag ggcgttgcca tcagcgccca
    34861 gtttgccgtc gaggcaaatc agatcgccct gtttggtgat gaccagcggg ttgatttcga
    34921 tcagcgccag gtcgcgctcc aggaaaatgg tcgccaggcc catgaagatt ttggtgaact
    34981 gctgaaccag tttaccttcc agacccagtt tgaacgccag ctcgcgtccc tgatacggca
    35041 tcgggccagt cagcggatca agcgcaactt tatggatcag gtgcggagtt tcttccgcca
    35101 ctttttcgat ttccacgccg ccttcggtgg aggccataaa gaccacgcga cgggaactac
    35161 ggtctacaac ggcaccgaga tacagctctt tagcgatatc ggtcgccgct tcaaccagaa
    35221 tctggttaac cggttggcca ttggcatctg tttgatacgt taccagacgc ttgcccagcc
    35281 agttttctgc aaaagcacgg atgtcttctt tgctgtttac aactttcaca ccgcccgctt
    35341 taccgcggcc accagcgtga acctgacatt tcactaccca cggaccggca ccgatttttg
    35401 aagcggcttc ttctgcttcg cgcggagtag tacaggcata acccaccggt gctggtaagc
    35461 catagcgggc aaacagttgt tttgcctgat attcatgtaa gttcatgtgt tctgtccatc
    35521 cttcagtaat cgttatcttt taaaccgtag accgacaccc tatccacgtc ggtctacagt
    35581 gcaggtgaag cttaaactac tacacgtcca gcagcagacg cgtcggctct tccagtaact
    35641 ctttgatcgt taccaggaag cccacggatt cgcgaccatc gatcagacgg tgatcgtagg
    35701 acagcgccag gtacatcatc ggcaggatct caacctgacc attcaccgcc atcggacgat
    35761 ctttgatagc gtgcataccc agaattgcgc tctgcggcgg gttgatgatc ggcgtagaca
    35821 tcagggaacc gaacacacca ccgttggtga tggtgaagtt accaccggtc agatcttcaa
    35881 ccgtcagctt gccatcacgg cctttgactg ccagctcttt gattttcttc tcgatgtctg
    35941 ccatgccgag ggtatcgaca tcacgcagaa ccggcgtcac caggccgcgc ggcgtagaaa
    36001 ccgccatgct gacgtcgaaa tagttgtggt aaaccacgtc atcgccgtcg atagacgcgt
    36061 tcacttccgg gtaacgtttc agggcttcaa ccaccgcttt cacgtagaag gacataaagc
    36121 ccagacggat gccgtggcgt ttttcaaacg cttcaccgta ctgcttacgc agatccataa
    36181 tcggcttcat gttgacttcg ttgaacgtgg tcagcatggc ggtggagttt ttcgcttcca
    36241 gcagacgctc tgccacacgc ttacgcaggc gagtcatcgg gacacgtttt tcactacgtg
    36301 cagccagagc cggttgcgcc gccggagcag ccgctgccgg agcagactct ttcgccgggg
    36361 ctttcgccag atgtttttcc acatcttcac gagtcagacg accacccaca ccggtgcctt
    36421 taatggcgct ggcgtcgaga ttgtgttcag ccagcaggcg acggatcgcc gggcttaacg
    36481 catcgttgtt ttgctcttcc agagacgcct gctggcgttg cgccggagtg gacgctttct
    36541 cttcagattt ggcgctggtt tctttaccgg cgctgttgcc ttcacgcagg cgaccaagga
    36601 tctgacgaga cgttaccgtt gtaccttcat cttccagaac cgcatccaga atgccgtctg
    36661 ctgatgccgg tacttccagt accactttgt cagtttcgat ttctaccagc acttcatcac
    36721 gtacgactgc gtcgccgggt tttttatgcc aggttgcgac ggtggcatcg gctacggatt
    36781 caggcaggtc agggaccaga atatctacgc tactcattgt gtatccttta tttattcgac
    36841 gttcagcgcg tcattaacca gatcttgttg ctgtttctgg tgaacggaca tataccctac
    36901 cgccggagag gcggaggccg ggcggcctgc ataacgcaga gaagccccaa acggaatcac
    36961 ttcacggaaa tgatgctggc tgcagtacca tgcgccctgg ttgagcggct cttcctggca
    37021 ccagacaaaa tccttgacgt gagcaaactg ctgcaacact tcctgcatcg ctttatgcgg
    37081 gaacgggtag agttgctcga tacgcacaat ggcgacatcg tgttgattgt tcttacgacg
    37141 ctgttccagc aggtcgtaat aaaccttacc agaacacatc actacgcgct tcacgccctt
    37201 cggatcaagc tcgtcgattt caccgatggc tggcaggaag gtgccgttcg ccagttcttc
    37261 gaggctggaa accgccagcg gatgacgcag cagggatttc ggcgacatca cgaccagcgg
    37321 acgacgcatc ccgcgcagcg cctgacgacg cagcatgtgg taaacctgtg ccggggtaga
    37381 cggtacgcaa acctgcatgt tttgctcagc acaaagttgc agataacgtt ccagacgcgc
    37441 ggaggagtgc tccggcccct gcccttcgta accgtgcggc agcaacatca ccagaccaca
    37501 catccggccc catttctgct cgccagagga gatgaactgg tcgataacca cctgcgcacc
    37561 gttggcgaag tcaccgaact gcgcttccca gatggtcagg gtgcgtggtt ctgcggtggc
    37621 ataaccatat tcaaacgcca gcacggcttc ttctgacagt acggagtccc agacacggaa
    37681 cgcgccctga ccgttatgga tgtgttgcag cggcgtgtaa gtggaaccgt tagactggtt
    37741 gtggatcacc gcgtggcggt ggaagaaggt accgcgaccg gagtcttcac ccgacaggcg
    37801 aaccggaatg ccttcatcaa ccagcgtggc gtaagcgagg ttttccgcac cgccccagtc
    37861 gaacagtttc tcaccggctg ccatcgcctg gcgatcgcca taaatcttgg caacgcgaga
    37921 ctgcatttca actgcttctg gtaccgtact gatacgttta gccagttcct gcaggcgctt
    37981 catctcaact ttgttcgggt actcttcgtc ccattcgtgg ttgaggtacg gcgaccaggt
    38041 gaaagagtgc atgttcatcg gacgccactc tgccactacg caatcgccag catccagcgc
    38101 atcgcggtac aggttaacca tctcggtggc atcttccagc gtcgccactt tttcctgctc
    38161 cagcttgtca gcgtagattt tgcgcggtgt cggatgtttt ttgattttct gatacatcag
    38221 tggctgggtt gcgctcggct cgtcggcttc gttgtggccg tgacggcggt agcataccag
    38281 gtcgatgaag acatcacgtt taaaggtgtt acggaaatcg agcgccagac gggtcacaaa
    38341 ggcaacggct tccggatcat ccgcgttaac gtggaaaatc ggtgcctgaa ccatcttacc
    38401 gatatcagta cagtacggtg tagaacgggc atccagcggg ttagaggtgg tgaaaccaac
    38461 ctggttgttg ataacgatac gtaccgtacc gccaacttca taaccacgcg ctttcgacat
    38521 gttcagagtt tcctgaacca cgccctgccc ggtcactgcg gcgtcaccgt ggatggtgat
    38581 tggcagcact ttgttgctgt tcggctcatc aagtctgtcc agacgggcac gaacagagcc
    38641 gataacgacc gggcttacaa tctcaaggtg agacgggtta aacgccagcg ccaggtgcac
    38701 caggccgcca tcggtctgga agtcagacga gaagcccatg tggtatttca cgtcgcctgt
    38761 gccgaggtgt tctttatgtt taccggcgaa ctcgtcgaac aagtcttgcg gttttttacc
    38821 cagcacgttc accagcacgt tcagacgacc acggtgcgcc atcccgagaa ccacttcgcg
    38881 ggtgccgctg ttgccagcgt ggcggatcat ctctttaagc atcgggatta acgcgtcacc
    38941 gccttccagc gagaagcgtt ttgcgccagg gaattttgcg ccgaggtaac gctcaaggcc
    39001 ttcagcggcg gtcagttcgc ttaagaagcg ttttttctct tcgctattga aagtcgcgcg
    39061 accagactcg atacgctgtt ggatccagcg tttttcttcg gtgctggtga tgtgcatata
    39121 ctcggcacca atcgggccgc agtaggtttg cttgagggcg gaaataagct cgccgagttt
    39181 catggtttct ttgccgctgg caaatgaacc gacgttgaag gtttcctgga agtctgcttc
    39241 ggtcagatcg tggaaagacg gatccagatc ggccacttta tcttgctgcc acagtcccag
    39301 cggatctaga ttcgcatgct ggtgaccacg gaagcggtat gcgttaatga gctgcaggac
    39361 tttaacctgc ttcacattgg tgtcagggtc ggagatcgtt gaagagtaac gtgaagcgtc
    39421 tttcgccagg cggcggaaat attcacgcgt ttgagagtgg aattgatccg gtttgactcc
    39481 cgtaccaggt aactgctgga acgtcgaacg ccagttagcg tcaaccgagt caggatcggt
    39541 taagaagtct tcatagagct gttctatcca gctctggttt gcgccagaga ggtaagaaga
    39601 gtccaaccag gctttcaaag cgctgttctg catcgtgatc ccttaagcat cttttttatg
    39661 cttacttcgc cgtggatact accacgcaca gtgatgtgcg tgccggggtt cacttgcgag
    39721 ctcttgtcta cgtagtggct cgcgaaggaa cctttaaaaa ctgtcttagt gtcaggggca
    39781 gtttttaaag atttcctgca tttctttgtt acgcctgatg cgctgcgctt atcaggcctg
    39841 cgggtaatga cttgtaggcc ggataaggcg ttcacgccgc atccggcact ggttgcctga
    39901 tgcaacgctt gcgcgtctta tcaggcctac agtttacgca ttacgttgca acaacatcga
    39961 cttgatatgg ccgatggcgc gcgtcgggtt cagccccttc ggacatacac tgacgcagtt
    40021 catgatgctg tgacagcgga atacgctgaa tgcatcgctc aaaccgtcga ggcggctgtc
    40081 agtctcggta tcacggctat cgatcaggaa acgatatgcc gctaacaagc ctgccgggcc
    40141 gataaactta tcgggattcc accagaaaga cggacaagag gttgaacaac atgcgcagag
    40201 aatacattca tacagcccgt cgagtttttc gcgctgctct ggcatctgta aatgctcgcg
    40261 agctggcgga ttttgtccat tattcaacag gtaaggctta attttctcat attgcgcata
    40321 gaattgtccc atgtctacca ccaaatcgcg gatcaccggt aaacctggca gcgggcgaat
    40381 cacaatcttc ttgcccggct ggttgagtgc cgaaatcggg gtaatacagg ccagaccatt
    40441 cttgccgttc atgttcagac cgtcggaacc gcacacacct tcacggcagg agcggcggaa
    40501 cgacaggctg ggatctttct ctttcagctg gataagcgca tccagcagca tcatgtcgcg
    40561 accttcttcc gcttccaggg tgtaatcctg catacgcgga gcatcatcaa catccgggtt
    40621 atagcgataa attgaaaact cgagtctcat tttcctgtct ccgcattagt aagtacgaat
    40681 cttcggcggg aatgccgggc gcagtttcgg ttccatgttg acgcttcggc gcgtcatgga
    40741 ttccgactca ggcagataca gggagtggca cagccagttt tcatcatcac gatccgggaa
    40801 gtcgaagcgg ctatgtgcgc cacggctttc ggtacggaag ttggcagaaa cagccgttgc
    40861 atacgccgtt tccatcaggt tatccagttc caggcactca acgcgctggg tgttgaactc
    40921 gctggaagtg tcatccagac gggcattttt cagacgctcg cggatcactt tcaactgctc
    40981 aagccctttc gccatcgcat caccttcacg gaagaccgag aagttatgct gcatacattc
    41041 ttgcagcgct ttacggatcg ccaccggatc ttcaccgtta cgattattgt tccaccggtt
    41101 caggcgatcc agagacgctt caacatcaga ctcgctggca tcgtgcagtg cgccctgctc
    41161 ggcgatagac tcttgcagat gcagacctgc cgcgcgacca aagaccacca ggtccagcag
    41221 cgagttgccg cccagacggt tagcgccgtg taccgataca caagcgattt caccaacggc
    41281 aaacagtccc ggaacaacca catcttcgcc tttctcattc acagtcagcg cctgaccggt
    41341 aactttggtc ggaataccgc ccatcatgta gtgacaggtt gggataaccg gaatcggctc
    41401 tttcaccgga tcgacgtgag cgaaggtacg ggaaagctcc aggatacccg gcagacggga
    41461 ttcgagaact tctttaccca ggtgatcgag tttcagtttc gcgtgtggcc cccacggacc
    41521 atcacagccg cgaccttcac ggatttcaat catgatggaa cgcgccacca catcacggcc
    41581 cgccaggtct ttggcgttcg gcgcataacg ctccataaag cgttcgccat gtttgttcag
    41641 cagataaccg ccttcaccac ggcaaccttc ggtgaccagt acgcccgcac cggcaatgcc
    41701 ggtcgggtgg aattgccaca tttccatatc ctgcaccggt acgcctgcgc ggatagccat
    41761 gccgacaccg tcgccagtgt taatgtgggc gttagtggtg gactgataaa tacgccctgc
    41821 tccgccagtc gccagcacgg tagcgcgggc tttgaaataa accacttcac cggtttcgat
    41881 gcacagtgcg gtacaaccca ccaccgcgcc atcctggttt ttcaccagat ccagcgcata
    41941 ccactcggag aaaatggtgg tgtggttttt caggttctgc tgataaagcg tgtgcaacag
    42001 tgcgtgaccg gtacggtcag cagccgccgc agtgcgtgcc gcctgctcgc cgccgaagtt
    42061 tttcgactga ccgccaaacg gacgttgata gatacgacca tcatcgagac gcgagaacgg
    42121 caggcccatg tgttcgagtt ccagaatcgc ttccggcccg gttttacaca tatattcaat
    42181 cgcgtcctgg tcaccgatat agtccgaccc ttttacggtg tcgtacatat gccattccca
    42241 gttatcttca tgggtattac ccagcgcaac ggtaatgccg ccttgcgcag aaacggtatg
    42301 ggaacgggtc gggaagactt tagagagcag ggcacaggtc tggccgctct gggaaatttg
    42361 cagcgccgcg cgcatacctg cgccaccggc accaatcaca actgcatcaa attctctgac
    42421 tggcaatttc atcacacacc ccacaccaca acgaatccat aaatcacgta aaccaccagt
    42481 gcaacgacaa tcaccagttg cagcatcagg cgcaaggcca gcggtttaac gtagtcggtc
    42541 aacacctgcc acatgccgat ccaggcatga atcaagatag aaaacagcgc cagcagggtg
    42601 aacactttgg tgaacgcaga ggcgaagaaa ccgatccaga cttcatatgt cagttcgcca
    42661 ctggtggcga aaaaaccgac catataaatg atgtagagcg tcaggacgat agcggtagcg
    42721 cgaacgagga tgaaatcatg tacgccattg cgtcctaatg cggaggcgtt gcttaccata
    42781 cgaggactcc tgcgagaagt gaaagcacga cagtaataac aaaggagatt ttggcggagc
    42841 gtttacccgc ttcgaatgtt tcttccagat agccaaaatc catcatcatg tggcgaatac
    42901 ctacgacgac gtgatacgcc agagcggtaa ggatgcccca catgataaat ttgacgaaga
    42961 agctgcccat aatcgcggaa gcttgctcga aaccttcagg ggaagagagg ctggtaccca
    43021 gaagccactg caggatgccc actgcaacaa aggtgatcac accggaaacg cgatggagaa
    43081 tggacgctat cgccgtgatg gggaaccgga tggtctgtag gtccagatta acaggtcttt
    43141 gttttttcac atttcttatc atgaataacg cccacatgct gttcttatta ttccctgggg
    43201 actacgggca cagaggttaa ctttctgtta cctggagacg tcgggatttc cttcctccgg
    43261 tctgcttgcg ggtcagacag cgtcctttcc ataactgcgc gtcatgcaaa acactgcttc
    43321 cagatgcgaa aacgacacgt tacaacgctg ggtggctcgg gattgcaggg tgttccggag
    43381 acctggcggc agtataggct gttcacaaaa tcattacaat taacctacat atagtttgtc
    43441 gggttttatc ctgaacagtg atccaggtca cgataacaac atttatttaa tttttaatca
    43501 tctaatttga caatcattca acaaagttgt tacaaacatt accaggaaaa gcatataatg
    43561 cgtaaaagtt atgaagtcgg tatttcacct aagattaact tatgtaacag tgtggaagta
    43621 ttgaccaatt cattcgggac agttattagt ggtagacaag tttaataatt cggattgcta
    43681 agtacttgat tcgccattta ttcgtcatca atggattctt tacctgcaag cgcccagagc
    43741 tctgtaccca ggttttcccc tctttcacag agcggcgagc caaataacaa acgggtaaag
    43801 ccaggttgat gtgcgaaggc aaatttaagt tccggcagtc ttacgcaata aggcgctaag
    43861 gagaccttaa atggctgata caaaagcaaa actcaacctc aacggggaca cagctgttga
    43921 actggatgtg ctgaaaggca cgctgggtca agatgttatt gatatccgta ctctcggttc
    43981 aaaaggtgtg ttcacctttg acccaggctt cacttcaacc gcatcctgcg aatctaaaat
    44041 tacttttatt gatggtgatg aaggtatttt gctgcaccgc ggtttcccga tcgatcagct
    44101 ggcgaccgat tctaactacc tagaagtttg ttacatcctg ctgaatggtg aaaaaccgac
    44161 tcaggaacag tatgacgaat ttaaaactac ggtgacccgt cataccatga tccacgagca
    44221 gattacccgt ctgttccacg ctttccgtcg cgactcacat ccaatggcag tcatgtgtgg
    44281 tattaccggc gcgctggcgg cgttctatca cgactcgctg gatgttaaca atcctcgtca
    44341 tcgtgaaatt gccgcgttcc gcctgctgtc gaaaatgccg accatggccg cgatgtgtta
    44401 caagtattcc attggtcagc catttgttta tccgcgcaac gatctctcct atgccggtaa
    44461 cttcctgaat atgatgttct ccacgccgtg cgaaccgtat gaagttaatc cgattctgga
    44521 acgtgctatg gaccgtattc tgatcctgca cgctgaccat gaacagaacg cctctacctc
    44581 caccgtgcgt accgctggct cttcgggggc gaacccgttt gcctgtatcg cagcaggtat
    44641 tgcttcactg tggggacctg cgcacggtgg tgctaacgaa gcggcgctga aaatgctgga
    44701 agaaattagc tccgttaaac acattccgga atttgttcgt cgtgcgaaag ataaaaatga
    44761 ttctttccgc ctgatgggct tcggtcaccg cgtgtacaaa aattacgacc cgcgcgccac
    44821 cgtaatgcgt gaaacctgcc atgaagttct gaaagagctg ggcaccaaag atgacctgct
    44881 ggaagtggct atggagctgg aaaacatcgc gctgaacgac ccgtacttta tcgagaagaa
    44941 actgtacccg aacgtcgatt tctactctgg tatcatcctg aaagcgatgg gtattccgtc
    45001 ttccatgttc accgtcattt tcgcaatggc acgtaccgtt ggctggatcg cccactggag
    45061 cgaaatgcac agttacggta tgaagattgc ccgtccgcgt cagctgtata caggatatga
    45121 aaaacgcgac tttaaaagcg atatcaagcg ttaatggttg attgctaagt tgtaaatatt
    45181 ttaacccgcc gttcaaatgg cgggttaatt tttatatgcc taaacacaaa aaattgtaaa
    45241 aataaaatcc actatcggac attcatatat tctaaaaaaa aatacatcgg tataaattat
    45301 aaacaaatag atgattttta atcatctatc ttcctctcag ttacatgaat aaaccaccaa
    45361 atatagcgca cccctataat tcccaatact ttattcatca aacatttaat tcataccatc
    45421 aattaattac ttttacattt aataaaatta ataatatttt taagaaaaac taaaaacatt
    45481 cgacaatcaa ataagatatt cttgcaccga tatacttttt tcttttctgt taaaggactc
    45541 agttatgttt aaaggacaaa aaacattggc cgcactggcc gtatctctgc tgttcacagc
    45601 acctgtttat gcggctgatg aaggatccgg tgaaattcac tttaaaggtg aagttattga
    45661 agcaccgtgt gaaatacatc aggatgatat tgataaagag gttgaactcg gtcaggtgac
    45721 caccagccac attaatcagt cacatcacag cgatgccgtt gctgtcgacc tgctcttagt
    45781 caactgtgat ctggaaaact ccagcaacgg ttccggtggc aagatttcta aagttgcagt
    45841 cacctttgat agctcagcga aaaccaccgg cgcagatccg attctcaaca acaccagcac
    45901 gggtgaagcc accggcgtcg gcgtacgttt aatgaataaa gatcaaagca atatcgtttt
    45961 aggcaccgct actccagata tcgacctggc tccgacctcc agcgaacaaa cgctgaattt
    46021 ctttgcctgg atggaacaaa ttgatcaggc tacacctgta acgccaggcg cagttacggc
    46081 gaatgcaacc tacgtgctgg attataaata agtattactt tggcggcgct atcgccgcct
    46141 ttcataaaat atttatcagg tatggacacc gtgaatattt atcgactctc ttttgtatcc
    46201 tgcctggtcg tggcgatgcc ttgcgcattg gcggtcgaat tcaatctgaa tgttctcgat
    46261 aaatcaatgc gcgaccgcat tgatatttca ttattaaagg aaaaaggagt cattgctccc
    46321 ggtgagtatt ttgttagcgt tgcggtaaat aataaccaaa tcagtaacga gcaaaagatt
    46381 aactggcaca aaaatgacga taaaaccatt ccgtgcatca atgatttact ggtcgataaa
    46441 tttggcttaa aacctgaagt ccgtcagtcg ttaccattga taaatcagtg cgtcgatttt
    46501 agctcccgac ctgaaatgct cttcaatttc gatcaagcca atcagcaact gaatattagt
    46561 attccgcaag cctggctggc gtggcactca gaaaactgga ccccaccctc cacatggaaa
    46621 gaaggtgtcg ccggtgtcct gatggattac aacttgtttg ccagcagcta ccgcccacag
    46681 gatggcagca gcagcactaa tcttaacgcc tatggaacag ccggaatcaa cacgggggca
    46741 tggcgcttac gcagtgatta tcagttgaat catactgata gcgatgataa ccatgaacag
    46801 tcaggcgaaa tatcacgtac ctatcttttt cgcccgttac cgcaattagg ttcaaaatta
    46861 accctcggcg aaacggattt tagtcccaat attttcgacg gtttttctta taccggcgcg
    46921 gcactggcaa gtgacgatcg aatgttgcca tgggaactac gcggctacgc cccacaaatt
    46981 agcggtattg cacagaccaa tgccacggtg acgatcagtc aatcaggccg cgtcatttac
    47041 cagaaaaaag tcccaccagg cccatttatc attgacgacc ttaatcagtc tgttcagggc
    47101 acactggatg tcaaagtgac ggaagaagat ggtcgggtga acaatttcca ggtttcagca
    47161 gcatcgacgc ccttcctgac tcgtcaggga caggttcgct ataaactggc cgcgggtcag
    47221 ccacggccct ccatgtcaca tcaaactgaa aatgaaacct tttttagcaa tgaagtttcc
    47281 tgggggatgc tgtcaaacac ctcgctgtac agcggcctgc tgctttctgg tgatgactac
    47341 cattctgccg caatgggtat tgggcaaaat atgctgtggc ttggtgcgct gtcgtttgat
    47401 gtcacgtggg ccagtagcca ttttgatact cagcaggacg agcggggctt aagctaccgt
    47461 tttaattaca gcaaacaagt ggatgccact aacagcacga tttccctcgc cgcttatcgt
    47521 ttctccgatc gtcattttca cagctacgcc aactatctgg atcacaaata caacgacagc
    47581 gatgcgcagg acgaaaaaca gacgatcagc ttatctgtgg gccaaccgat taccccacta
    47641 aacctcaatc tttacgccaa cctgctacat caaacctggt ggaatgcaga cgcctccacg
    47701 accgccaaca tcacggcagg ttttaatgtt gatattggtg actggagaga tatctctatt
    47761 tctacgtcat tcaatacaac ccattacgaa gataaagatc gcgacaacca gatttacctg
    47821 tcgatttcgc tccccttcgg taacggtggt cgggttggct atgacatgca aaacagtagc
    47881 cacagcacca cacaccgcat gtcgtggaac gatacgctgg atgaacgtaa tagctggggc
    47941 atgtctgccg gactgcaatc cgaccgtcct gacaatggag cccaggtgag cggtaactat
    48001 cagcacctga gttcagcggg tgagtgggat atttctggta cctatgccgc caatgattac
    48061 agttccgtca gcagcagctg gagcggttct ttcaccgcaa cccaatatgg tgcagcgttt
    48121 catcgccgca gctccaccaa tgaaccacgc ctgatggtca gcaccgatgg cgtggcagat
    48181 attccggttc agggcaatct cgactacacc aaccattttg gcattgcggt ggtgccgttg
    48241 atttccagtt accagccttc caccgtggcg gtgaacatga atgacttacc cgacggcgta
    48301 acagttgcag aaaacgttat caaagaaacg tggattgaag gcgcgatagg ttacaaatca
    48361 ctggcttccc gttccggtaa agacgttaac gtcatcattc gcaacgccag cggtcagttt
    48421 cctcccctcg gagcggatat ccgccaggat gacagcggca ttagcgtggg gatggttggc
    48481 gaggaaggac atgcctggtt aagcggagtc gctgaaaatc aaaagtttac cgtggtctgg
    48541 ggtgatagcc agcattgctc gctccatctt cctgaacata tggaagacac cgcaaatcgc
    48601 ctgattttac cttgtcatta attaaggaaa tgaaaatgac atttatgaaa ggactacccc
    48661 ttttgttgtt ggttgccagt ctgtgcagcc acgctgcact acaacccgat cgcactcgta
    48721 ttgtgtttaa cgctaatgat aaagctacca gtctgcgggt ggataatcgt agcgataaac
    48781 tcccctacct cgcttattcc tggctcgaaa atgaaaaagg ggagaaaagc gatgatcttc
    48841 tggtcgccct gccgccgata caacgtctgg aaccgaaagc aacaacacag gtacggatag
    48901 taaagcaagc ctcgaccaca aaattaccgg gcgatcgcga aacgctattt ttctacaata
    48961 tgcgtgaaat tcctcccgcc ccggaaaaaa acagcgacca tgccgttctg caagtcgcca
    49021 ttcagagtcg tatcaaggtg ttctggcgac cggctgcatt acgcaagaaa gcaggagaaa
    49081 aggtcgaact gcagttacag gtcagccagc agggcaacca actgacactg aaaaatccta
    49141 ccgcgtatta tctgaccatt gcctacctgg gacgaaatga aaaaggcgtt ctccccggct
    49201 tcaaaaccgt gatggttgcg ccttttagta cggtcaacac aaacaccgga aattatagcg
    49261 gcagccagtt ttatctcggt tatatggatg attacggcgc gttacgcatg acgacgctca
    49321 actgcagcgg acaatgccat ttacaagccg tggaggcgaa gaaatgagtg ctggcaaggg
    49381 agtgttgctc gtcatctgtc tgttatttct gtcgttgaag tctgcgcttg ccctgaactg
    49441 ctactttggt tcatccggtg gctcggtaga aaaatcagaa gcgattcaac cttttgctgt
    49501 accaggcaat gccaaactcg gcgataagat ctgggaatct gacgatatta aaataagatc
    49561 tgggaatctg acgatattaa aattcccgtc tattgtgaca acaataccaa cggcaacttt
    49621 gaaagcgaac atgtctacgt atgggtaaac ccctacccgg gggtgcagga tcgttattat
    49681 caactcggag taacttacaa tggcgttgat tatgacgcca accagggaaa aagccgcatc
    49741 gacactaacc agtgtatcga cagtaagaac attgacattt atacccctga tgggatggca
    49801 gaacaaaatt tgctcgggtg atcccgctaa tattcacatg tcgcgaacct ttctcgctcg
    49861 catgcggtta tacgtcaaaa tacgagaaat gccgccgcat gattatcaaa gtacgcttag
    49921 cgactatatc gtcgtgcaat ttgacggtgc cggtagcgtt aacgaagacc ctactgccca
    49981 aaacctgaaa tatcatatta ctggtctgga aaacattcgc gtgctggatt gcagcgtcaa
    50041 tttttccatt tcaccggaaa cacagatgat tgattttggt aaatttaatt tgctggatat
    50101 tcgtcgccac acaatgtcga aaacattcag tattaagacg acaaaatcac aaaatgacca
    50161 atgcaccgac ggatttaaag tcagttcctc cttttatacc gaagaaacgt tggttgaaga
    50221 agataaagca ttgttgattg gtaatggtct gaagctacgt ttgctggatg aaaacgcctc
    50281 gccttatacc ttcaataaat atgctgaata cgccgatttc accagcgaca tgttggtcta
    50341 cgaaaaaacc tatacagctg aactttcgtc cattgcaggt acccccatcg aggctgggcc
    50401 cttcgataca gtggtgcttt ttaaaattaa ctataactga tttgccatga tgccctttgc
    50461 agccggaaag ggcatctctt tcagaaaatt gctgattttc cccttttcct tgattgctgt
    50521 tattttttcc tagcccggct ttctttatgc tgtatcgata caccaaagag aaaataatga
    50581 gggagcgaag gatgccagtt ttgcagtggg gaatgttatg tgtgctatca cttctccttt
    50641 ctattggttt cctcgcggta catctcccgg cggcgctatt acttgggccg atgatcgccg
    50701 ggatcatctt cagtatgcgc ggaattactc tgcaacttcc ccgctccgct tttctcgccg
    50761 cgcaggccat tcttggctgc atgattgcgc aaaacctcac tggttcgatt ctcaccacgc
    50821 tggccgccaa ctggccgatc gtgcttgcta ttttactggt gacgctgctt tccagcgcca
    50881 tcgtgggttg gttattggtg cgctatagct cactgcctgg aaataccggt gcctggggtt
    50941 cttctccagg cggcgcagcg gcaatggtcg ctatggcgca agattacggc gcagatattc
    51001 gcctggtggc gtttatgcaa tatctgcgag tgctgttcgt cgcgggggcc gctgttctgg
    51061 tcacccgcat gatgctgggt gataacgctg aagcggttaa ccagcaaatc gtctggttcc
    51121 cgccagtgag cattaatctc ctgcttacga ttttgctcgc agtcgttgcc ggtacggcgg
    51181 gatgcatgct gcgtcttcct tccggcacga tgctcatccc gatgctggcg ggcgcagtgc
    51241 tccagtctgg tcagctcatc accatcgaac taccggaatg gctgctggcg atggcgtata
    51301 tggcaattgg ctggcggatt ggtcttggtt tcgataaaca aatcttactg cgggcactac
    51361 gcccgctacc gcaaatcctg ctgtcgattt ttgctctgct ggctatttgt gcaggtatgg
    51421 cgtggggact gacccggttt atgcatattg attttatgac tgcctacctc gccaccagtc
    51481 ccggcgggct tgataccgtg gcggtcatcg ccgcagggag caatgccgat atggcgctta
    51541 ttatggcgat gcaaaccctg cgcctgttca gtattttgct gacggggcca gccattgcgc
    51601 ggtttatttc aacctatgcg ccgaagcggt cggcctagtg ctggcagcct ggacaccagt
    51661 aaaacgggcg cgaggacaac gtggttttct caatgatgcc gccacagcgt tcgcacagtt
    51721 cgccatcacg atgaaaaacc ttaaagcgaa acagcgcccc atgatgctta ttctcatcca
    51781 cctgcccccg cgtagcgtaa gaaaaccgag gaatctccag taacgcgtgt gcgagtgcat
    51841 ccagttgcgc cgcattgaga tctttcgctt tatgatttcc agtcaaccca acctgccaga
    51901 ggatctccac ccgcaaataa ttgccaagcc cagccagaaa cgcctgatcg agcagtaatc
    51961 ctgcaaactg acggttacga aagcgcggcg acaataaccg ttcctttacc acctccggcg
    52021 tcagattcgg atccagcaca tcgggaccaa cgcgttgtaa aaacggatgc gtggtcagtt
    52081 gttccggcct caacatctca atatccgagg cgctataaag cagaatggtt ttgtcagccg
    52141 tttgcagttt tacccgcaat actcgcgtgg tctgcggctc ttcgccggta tcaaccacgc
    52201 gccagacgcc gtaaagctga ttatggctgt agagcgttaa accgttggga aaatgagtta
    52261 acaacgcctt accacgcgtt tccacatggg taacgtgttg accgataagt tgtgattgat
    52321 aagtttttaa ctgcgggaag gcaaaccaga catcagttag tggtttgcct ttgatcgccg
    52381 cctccaggtt atccgctgca cggcggatct ccgggccttc aggcatggtg atatcctttt
    52441 ttcattcttt attaagcggg gttatgctgc gacaacaatc ccctttttgg caaatgtagc
    52501 tcgtaatcgg cgggcgaacg ccagagcgtg ctcgccgtcg ccatgcaggc agaccgtttg
    52561 cgccgcgacc gttgcccatt cgccggtgat acttttgact ctgccgtgtt gcaccatctc
    52621 cagcgtttgc gccaacgcct gttcttcgtt ttcaatcaac gcgcctggct gacttcgcgg
    52681 caccagcgag ccgtcagcct gataaccgcg atcggcaaac acttcctcgc gcgttgtcag
    52741 gccatattgc ttgcctgcac gaatcagctc gcttcctgcc agcccaacga gaaccaatgc
    52801 cggatcgcaa gcgtataccg ctctggcgat ggcgtctgcc agttgtgctt ctttcgccgc
    52861 ctggttgtac aacatgccgt gcggtttgac atgacgcatt acgccgcctt gcgcacgggc
    52921 aatcgttgcc agcgcgccaa tttgatacag cgtctgggcg taaacggttt ccggcggcag
    52981 ctgcatggcg ctgcgaccaa aattttccct gtcgggaaaa ctcgggtgag cgccaatcgc
    53041 gacaccattt tttattgctt cacgcacgca agcctgcatg gtttgcgcat cgcctgcatg
    53101 aaatccacaa gcaatattgg cagaggaaac cagcgttaat agctctgcgt cgctggcgca
    53161 gccttcgccc agatcggcgt tcaggtcaat tttcattgtg cagccgccac gctaattgtt
    53221 caaaataacg ttgctgatct tgccgcgctt tcagtgcctc ttccagtgaa cactggacaa
    53281 aatgaatcgg ctgaccgagc ggaatttgcg ccagatggta catatcagcc tcaatgatac
    53341 aggcaatacg tggataacca ccggtggtct gtgcgtcgtt catcaacaca atcggctgcc
    53401 cgttatgcgg cacctgcacc acgcccggta acaaaccgtg agataacagt tcgcgatcgg
    53461 tgatgcgttt taaaatttgc ccctgtaaac gatagcccat tcggttactt tgcgagctaa
    53521 gctgccaggg cgaacgccag aatgcatcct gcgaggcgcg atcgaactca tgatattccg
    53581 gccccggcaa ggcgcgaatg cggttgcccc acagcagctg tttaacgccc tgcgcttcca
    53641 taaaatcacg cttcgatttg ccaatcggga gtcagtcacc atccttcagt aaacggcctt
    53701 ccagcccgcc aatccccact ttgagatcgg tgctgcatga ccccattacc ggcggaacat
    53761 caataccacc cgcgaccgcc agataactgc gcatcccgtg ctgcgggcgt ttaagcgtta
    53821 aacgctggcc tgctttcatc ggcaatcgcc agccggtcca gacggcgtta tcatccaacc
    53881 gcgcttcgca acctgcaccc gtcagggcaa accacccatc agtttcgaac tcaactgtta
    53941 actgaccgag cgtgatctcc agcgcggggg cattggcgtc attacccacc agcaggttag
    54001 caatgcgcag cgcaggcata tccagtgcgc cacagtggct gatacccgac tggcaaaaac
    54061 cgtgacgacc gccatcctgc acagtggtgt acatgcccgc acgaataatc ttcagcatat
    54121 tccctccttc tgcggtacaa atcgcacgct gtctcccgga cgtaataaga tgggttcgtc
    54181 acgcgccgga tcaaacaggc tgagtgaggt atgaccaatc aactgccagc cacccggcgt
    54241 tgccagcgga taaacaccag tctgcggccc gccgatcccg acagaacctg ccggaacgag
    54301 caagcgcggt tcagcgcgcc gtggcgtgtg tagttgttcc ggcaaactcc cgagatacgg
    54361 gaagcccggt tgaaaaccta aaaaccagac cacgtattcc acggaggagt gcaattcaac
    54421 aacctgtttt tcgctcaacc cgcaatacgc cgcgaccacc gccaaatccg gtccgcctgc
    54481 accaccgtaa accaccggaa tttcaataaa gcgagactca ggttccaggg cctcgctctc
    54541 ctcccaccag cgttgcaaac gctctatggc atccagcgcc agcgactcag gattacgcaa
    54601 aatcaccgtg atattgttca tgccgggaat ggcttcaacc acattcggca tatccaccag
    54661 acgctgcgcc agtcgccaga tccgtttctg gctagccagc gtcaccggcg gttccagttc
    54721 cagcactacc gccgtttcac ctatcagata acaacgcgct cgttgcactt tcgatccctc
    54781 tgattattcg ttatgcagga ttaggaatat caataaaggt cacatcaaga tcggtatttt
    54841 cattcagcca ctcgctcaat gcgcgaatac caccgcgttc agtggcatgg tgacctgcag
    54901 cataaaaatg caatccctgc tcgcgggctg aatgaatggt ctgttcagaa acttcgccag
    54961 taataaaagc atccacgcca aaacgcgcgg cgctatcgat aaaactttgc ccgccgcccg
    55021 tgcaccaggc gacgcgctgg accacctcag gtccggtatc gccacaccat aacggcttac
    55081 gtcccagacg cgcttcaatc caggaggcta gttccattcc cggcacaggc atggtcagct
    55141 ctccccacgg caccaacggc tcaatttcgc ccatgaccgt gatccccagt aacgccgcca
    55201 gctgtgcgtt attgcccagc tcaggatgtg cgtcaagcgg caaatgccag ccatacaggt
    55261 tgatatcatt cgccagcaac gtttttaaac ggttacgctt cattccgcga atgaccggag
    55321 actctccttt ccagaagtag ccatgatgca caatgaccgc atccgcgccc agacgcactg
    55381 cctcatcgag caaagcctgg ctggcggtga caccggtaac aattttttgc accgtctctt
    55441 tgccttccac ctgcaaacca ttcggcgcgt aatcactaat cgccgcgctg ttcagttttt
    55501 cgttaatcag ttgttccagt tcggtgtttt tcatcttcgc gccttccgta ttctgatagc
    55561 tacaccgtta acatagcgtt acatcgcctg ttcgtcgata gccaatcttt tgacatatta
    55621 agaaacaaac ccgacgccaa aagacgcaaa ttgtttatat catcacgctt cagctaatgt
    55681 atatttatgc aatcagacag catattgcat aaataatcag atctgtatca aagttttaac
    55741 tttaaggaaa cacaagcacg ccgcgcaact attacgtgac gttgccgtca attgtggaat
    55801 tattatgaat aaacacgcat cacaaccgcg cgctatttac tatgtcgttg cgctgcaaat
    55861 ctgggaatat tttagctttt acggcatgcg tgccctgctg attctctatc tcaccaatca
    55921 actaaaatac aacgatactc acgcctacga gttatttagc gcctactgtt cgctggtgta
    55981 tgtcacgcca atcctcggtg gctttttggc ggataaagtt ctcggcaatc gcatggcggt
    56041 gatgctgggg gcgttgttga tggcgatcgg tcatgtggtg ctgggtgcca gtgagatcca
    56101 tccgtcattc ctctatctgt ccctggcgat tatcgtctgc ggctatggcc tgtttaaatc
    56161 taacgtaagc tgtctgctcg gcgagctgta tgagccaacc gatccgcgtc gtgatggcgg
    56221 tttctcgctg atgtatgcgg cgggtaacgt ggggtctatt atcgcaccta tcgcctgtgg
    56281 ttacgcccag gaagagtaca gctgggcgat gggctttggc ctggcggcgg ttggaatgat
    56341 cgcgggtctg gtcattttct tatgtggcaa tcgtcatttc actcataccc gcggcgttaa
    56401 caaaaaagta ctgcgtgcga caaactttct cctgccgaac tggggatggc tgctggttct
    56461 gctggtggca acgcctgcac tgattaccgt gctgttctgg aaagagtggt cggtatacgc
    56521 cttaattgtc gcgactatta ttggcctggg tgtactggca aaaatttatc gcaaagcaga
    56581 aaaccagaaa cagcggaagg agctggggct gattgtgacg ctcaccttct tcagtatgtt
    56641 gttctgggcc ttcgcacaac agggcggtag ctcgattagc ctttatatcg accgcttcgt
    56701 taaccgcgat atgtttggtt ataccgttcc gaccgcgatg ttccagtcga ttaatgcctt
    56761 cgcagttatg ctgtgcggtg tgttcctggc gtgggtggtt aaagaaagcg tcgcaggtaa
    56821 tcgtaccgtg cgcatctggg gaaaatttgc tcttggcctt ggcctgatga gcgccggatt
    56881 ctgcattctg accttaagcg cccgctggtc cgcaatgtat ggtcactctt ctctgccact
    56941 gatggtgtta ggcctcgcgg tgatgggctt tgcggaactg tttatcgacc cggttgccat
    57001 gtcgcaaatt acgcgcattg aaattccggg cgtgaccggc gtattaaccg gcatctacat
    57061 gctgctttct ggcgcgatcg cgaactatct ggcgggcgtg attgccgatc agacatcgca
    57121 ggcttcgttt gatgcttccg gggcgatcaa ctactccatc aatgcatata ttgaagtatt
    57181 tgatcaaatt acctggggcg cactggcgtg tgtaggactg gtactgatga tttggctgta
    57241 tcaggcgctg aaattcagaa accgcgcgct ggcgctggag tcataatagg ccagcaccga
    57301 tgcctgatgc gacgcttgac gcgtcttatc aggcctacgt atttcctgca atttattgaa
    57361 tttgcacaaa tttgtaggct ggattaggcg ttcacgccgc atccggcatc tgggctcgat
    57421 tgcctgacgc gtctgttatt tccccttccg cgccgcctca tacgctgcca acgtttgtac
    57481 tctcgcctct ttgtgctcga ctatcggttg cggataatcc agcttcacac ctgctttctg
    57541 cgcccacttc cacggctcat gcaccgcttt ccctggtaca tcgcgcagct ccggtaacca
    57601 tcgacgaata aactcgccct cacggtcaaa tttctcgccc tgagttatcg gattgaaaat
    57661 acgaaaatac ggcgcagcat cagtacccgt agaagccgcc cactgccagc caccgttatt
    57721 agctgccaaa tcaccatcaa tcagctgcga catgaaatat cgctcgcctt cgcgccagtc
    57781 gatcaacaaa tctttcacca gaaaactggc tgtaatcatc cgtagccggt tatgcatcca
    57841 gccagtgctg ttaagctgac gcatagcggc atcaataatc gggtatcccg ttttgccttt
    57901 ctgccaggcc tgtaaatgtg cgggattgct ttgccactgc acacgatccg tccaggcaat
    57961 aaatggacga tgtttacaca acgaggggta atacgtcatc agatgacggt agaactcgcg
    58021 ccagatcagc tcactaagcc agacactacc ggccccaccg tccagcgcct gcggctgttc
    58081 cgccaacaag cgatgcaaac actggcgagg cgataacccg cccgttgcca agctggcgga
    58141 caaacggctg gtgccttcca ctgccggaaa atctcgttgt tgctcatact ctccagcacc
    58201 gttctcgcaa aactggcgta attgcgcaat cgccgctttt tcttccaccg ggaaatgcgc
    58261 agtatcgaaa gactgacgag gataattcag cgtaatggat ggcgcgggct ttatcgatcc
    58321 gctactacga acttttggtg cagcgacgca ctccggcatc ccttcccgca gccgtttcag
    58381 ccaggcattc ttaaaaggcg taaagacttt gtacatctcg tgattgccgg tcatcaccgc
    58441 ccccggcggc aggatcacgc tgtcatcaaa tccttcacac accacgttac gcagcgctct
    58501 ttcaacgtcc acatctcgcg ctcgctcatt cacctcatac tgatagttat aaaaaagatg
    58561 agtaacgctg ttttccgcgc acacctgttt aactatttcg acactggcga caaagtcatc
    58621 cacttcacgg aacaataaag gaataccttt ttccgccagc gcgatttgta gcccattcag
    58681 ttgagcattg ataagttcag cctgacgcgg cgacatgtta tgcgtctccc acttgcgtgg
    58741 tgtagcgata tacagcgcca gcacgcgtgc agacgaattg cggcaggcgg cagccagtgc
    58801 gagattatcg tgcagacgta aatcctggcg aaaccaggcc agatgggtag tcataaaact
    58861 cctgaatggc gcaagttaat cacacgcggc caaagttcga aataacgctg ccctgaaagc
    58921 caggcgtcag gataaagcgc catatagtgt ttgatacgca tcagcggcgc aagaagtggt
    58981 tgctcgccac ggcgataact gtcgataagc gcagccagct gctggcgttc tgtggaatca
    59041 atatgcgggc gaaaataacc ctgaacgtgc atcaaaacgt tggtgtgatc gcgcgggttg
    59101 gcgggatgag aaagcaaaac aattacgcgt tggcggtact ggttgtaaaa atcatccaga
    59161 ttttgccact gatgaatatc agccacaaac gggccaagtt tgcggtactc gggctgagaa
    59221 tgggccagaa agacgagttt atagcggctg tgataatcga gaagcgcccc gcgcgttaag
    59281 ccatgttctt tcagtcgatg taactcatga agggcgaaga tacggattag ggtgctgtca
    59341 tcaaatcgtt gtagattcat tactgctcct gaacggtata caggagtaag tgtaggcgtt
    59401 ggtaaatgat gtttgtgaag ttaatccgct agcagggcaa gattacgaaa gcgcgctccc
    59461 cgcaagcaac taatcaaggt agttaatcat ggctgaacac gatattacgc cagaagtcac
    59521 tatttccaaa acacagcgcc gctataaagt cggctatgtc agagcccgcc accaggaccg
    59581 caaaaccggt atagcccggt attattcaca acatcccagt ctgcacctta agggcgactg
    59641 gctgaaagag gccggatttg ataccggacg gggtgtcacg gtgaagattt cggagggggg
    59701 cctgaccatc atcgctgaca gtgacgaggt gcaggaactg cgggatggga tgtttagtgt
    59761 gctgaacgaa agttaaatat taaaaaagct gggaagattt aaaaccttcc tagcttttta
    59821 ttctgtctta ttataatgga tactcatcat cttccgatga atttataaat tgctccaagt
    59881 ttacatgaag agtacttaac ctagttttta actcattaag tgaagcgata atttcatatt
    59941 ttccaacata atcatatgat ggatcgtatt cattatctat gtcagaagct aaaaatgatg
    60001 atattcgtag tttaaattta gcttcatctt tattacattc attggcataa ggtgaatgcc
    60061 ctatcatagc atattcatct aatctaaaag gataagatgg ttttaattca tcgatcaatg
    60121 aaatttcaat ccatgttcta tatccaggca ttaatggact aatttctttg agcaaaacct
    60181 ttccacttaa taatgtattg agattgttca ttattatgtc tcccacggga aaaatatatg
    60241 tctaacactt ccaccatgtt gatgaacttg taaatgtggt aaatcaccat caggatttcc
    60301 tggcccatga cctgacactc tatatgaagg ttttgttaca gaaggatctt ttatatcatc
    60361 ccaatgatat gaacctgctt ttgttttatt ttcagtaaga tattccatta agttcttagt
    60421 gctggggcct gactcacctg tcatattttt ataattatga cctaaatatc tatccataaa
    60481 caattgttca gcgtcacttc tagacctaac agtaactttg gtttcactac tatctaataa
    60541 acgattcgct ttttggttac taacagcaca tggagttaat ccaagtggat cagcatattt
    60601 tagaggatta ggtgcataac tgtagagatt taaaccgcct ctcaacccta tcgggtcagt
    60661 taccatgaac cgcccgacat ccggatcata gtacctgtgc agattgtagt gcagccccgt
    60721 ttccctgtcg aggtactgtc cctggtagcg caggttctgc tccaccccgc cgtggtcctt
    60781 ctcctgtatc gtgttacccc agacctgata ccccgtctcc cacacgattt tcccgtcgct
    60841 gtccgtcatc tcttccggcg cgccgttcac atccgtgtga aaatacagta tccggttcgg
    60901 gccctctttg cccgccttat ccacccgcgc cagcggttca taactcccct ggtccctgta
    60961 gatgtacagg ctgcctgttc cctccggcct gctctcctgc gccagccgca ttccgtccca
    61021 taaaaatttt gtccggttgt acggcttacc gtcagggctg atgtgctgtt tttccgtccg
    61081 cctccccagt gcatcgtaca cataccggta tgtcccccgc gctgtcgtca cttccaccat
    61141 ccggtgttcc gcatcataac ggtaactctg cgttccgctc cggccctcct tttccaccgc
    61201 acggccaaat tcgtcataac ggtagtgcac accacggtaa gacatcagcc ggttgaacgg
    61261 caccacgctg ttttctgccg tggccctttc ccccgcgcgc ctccccagca ggtttcccgc
    61321 cgcgtccacc tccgtttccg gctggtgcca caccgggtgc cactgcgcct gctccacccg
    61381 ccccagcggg tcgtgcgtct ccgtgatatg cccctgcagg tcatacacat agcgccattt
    61441 gccgccctga gggtcggtca tccccagcaa cagtcgttct tcgtcgctcc agcggaatgt
    61501 cgtcacctgt ccggcttcgt cctggtacgc cgtcacctga ttctgcgcat cccactggtg
    61561 ataacgtgtg cgccccagtc cgtccgtcac gcacagcctg cgctgcgcca ggtcccacgc
    61621 cagccggtac tgttcaccgc tgtccgtgtg gtgccccgtc acccgccatg ccgcgtcatc
    61681 cggaccttca taccagcccc actggtaata atacccttcg ccgcccggct ggcggtgcag
    61741 cgtcatgcag tggttttcgt cgtaagcaaa ctcacgcagc aggtgccccg tgctgtcgta
    61801 cacgccgttc aggtggccgg cctcgtcata gctgtaacga cgcagcagta aaggtgcagt
    61861 ctcagggaaa tgctgataaa tctgtgtcac ccgccgggga tgcgccgcca gttcgtaata
    61921 cagacggatg cacggacgct gctgttcgcc gctgatttcc acaatgcggc cccggtcgtc
    61981 gtagtacaga ttcagacagt tgctgttacg gtcgcccagc attttcagcc gctggcgctg
    62041 cgggtggtgc gggtcagcct caaacagaaa acactgcccg tcatcgctgc tgataagcca
    62101 gtgcccctgc tcaccacgcc ttaccgccag cccttcaccg gggctgtaaa acccgccccc
    62161 cggcagtacg gcttccatat ccaagcgtcg ccccatgccc gacacaaagg tcatgcagtt
    62221 ttcgtctgcg ctacccggtg tgcacacgag gctgacttca tacattacac tccagccctg
    62281 accaaacatt ccttccgtac gttcgtcacg actgctgtac acgcgctgcc agtccagcgg
    62341 aaagtggccc ggcagactga aatcgaagtc atcgtcatcg gccaggtatt tggccccggt
    62401 ggcggcattg accggatgcg gaagggcact ggtggccttc tgcgtcagca tgctcatccc
    62461 gacactggca gcaaaacagg cgattttaga aagcattttg cccgggcgaa gaaacatcag
    62521 cgccacggtt gccaccaggg ttatctgcga cctgccgctt ctgatatccc tgaccaccag
    62581 taacggaccg ccaatgcgca catccggtga cacaaacgcc ccgttccccg gctcattcac
    62641 caccctggcc tcgcaggtac agcgtgcccc gctgcgtgtc gccggctggc tgttgatacg
    62701 cacatcccgc gcgccttccg cgatatattc cgctgcgggc ggctcgtcat cgcctttgaa
    62761 cagattactg atgctgccaa taaccgcatt cactccctga aaggcggttt ccagcatacc
    62821 gcccggattc agcaggttaa gggccgttcc cagggggtca tccagaaagg cggctttttt
    62881 ttgtgcgaga aactgcgtca gtgaggaagg atgtttatgg cagtcgattt tatcgtcagg
    62941 gggcggggtg gatcccgggg ccgcactggc cacagtgggc tgccatagtt ccgacacaaa
    63001 atgtgtcacg ccatccacca tttcagaacg cccttcttcc gtactgagct ggtcgtgcag
    63061 agcccgggac atcagcacag gccctgtcac cgggttaacc gccagggcta tggcccccgc
    63121 catttccaga tattccccgg ccacatcccg gaacgttctg gcatcctcct gacgctgcgc
    63181 ttcttcctgt tctgccttcg cctgcgcttc ctgcgcctct atgtcctgcc gcgatgccgc
    63241 agtcgccgct gcccgcgcgg caggtttgct gttgatccat gtgtcaccag agccggtgag
    63301 gatgtaacct tcaatctgag gcgggaaaag catatttgcg agggctgaac aggcttcgct
    63361 gaccttttct gtcacgccgg tgtacaccat cgccacaccc gccacaaaac cggtcagcac
    63421 gacggccgtg caccccatcg tgatccccac tgacgtcgcc agtaacgagg ttgtgccgac
    63481 aatgagggca tcaataacga catttgcggc cacctccagc accgccccca gcacatctgc
    63541 cataaacgac gtatgcagca gggcatcacc ctgtcttgcc gcccagaatg cctcttccat
    63601 gtcccgttcc ctgtatgatt aattcgtcct gacttcctgc cgcatcctga agcgcagatt
    63661 ctttttaatt tcagcccagc gcgtcatatc tgccggggtc agcggtgtgg ttctggcgta
    63721 ggtgaaggcc atcatgaccc gctcacccgg caatacaaat gccacctgac actggtacac
    63781 ctgctcgtta ccccggagaa actggctctg tatttccaaa ccgcctcgtt gagataatat
    63841 tccgtcataa tgtaaatagt ccgttttaat aaaggaatta tttattgaca ggttctgttt
    63901 ttttaatcat gaatgtaata cccatcaccg ctttacagca cactttatct ttccagtcat
    63961 aatcactaat aatattaagc aattagcaat atttacaacg accagagcgg atacatagtt
    64021 atgtatttaa caatcccaat atattcacta tgcaaataaa accactagta acagacacgc
    64081 taccgcctcc atataaaaat ggacacctta taaaattgta tcttgcatag aaaaagctga
    64141 aatatcggat gcgttttaat tatcaactat aaatctataa gctttttatt aacaac
//
LOCUS       DAJTSF010000010        63060 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000010 DAJTSF010000000
VERSION     DAJTSF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.010"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..228
                     /locus_tag="OO335_000699"
     CDS             <1..228
                     /locus_tag="OO335_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR6001195.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            623..1262
                     /locus_tag="OO335_000700"
                     /pseudo
     CDS             623..1262
                     /locus_tag="OO335_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1365..1688
                     /locus_tag="OO335_000701"
     CDS             1365..1688
                     /locus_tag="OO335_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR6001196.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OO335_000702"
     CDS             complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OO335_000702"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6001197.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OO335_000703"
     CDS             complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OO335_000703"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6001198.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OO335_000704"
     CDS             complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OO335_000704"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6001199.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OO335_000705"
     CDS             complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OO335_000705"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6001200.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OO335_000706"
     CDS             complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OO335_000706"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6001201.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OO335_000707"
     CDS             complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OO335_000707"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6001202.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OO335_000708"
     CDS             complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OO335_000708"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6001203.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OO335_000709"
     CDS             complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OO335_000709"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6001204.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OO335_000710"
     CDS             complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OO335_000710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6001205.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OO335_000711"
     CDS             complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OO335_000711"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6001206.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OO335_000712"
     CDS             complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OO335_000712"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6001207.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OO335_000713"
     CDS             complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OO335_000713"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6001208.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OO335_000714"
     CDS             complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OO335_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6001209.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OO335_000715"
     CDS             complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OO335_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6001210.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19267..20484
                     /gene="alaA"
                     /locus_tag="OO335_000716"
     CDS             19267..20484
                     /gene="alaA"
                     /locus_tag="OO335_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6001211.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20568..21167
                     /gene="yfbR"
                     /locus_tag="OO335_000717"
     CDS             20568..21167
                     /gene="yfbR"
                     /locus_tag="OO335_000717"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6001212.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21226..23058)
                     /locus_tag="OO335_000718"
     CDS             complement(21226..23058)
                     /locus_tag="OO335_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6001213.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OO335_000719"
     CDS             complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OO335_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR6001214.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23806..24300)
                     /locus_tag="OO335_000720"
     CDS             complement(23806..24300)
                     /locus_tag="OO335_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6001215.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24383..24838)
                     /locus_tag="OO335_000721"
     CDS             complement(24383..24838)
                     /locus_tag="OO335_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6001216.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25176..26378
                     /gene="ackA"
                     /locus_tag="OO335_000722"
     CDS             25176..26378
                     /gene="ackA"
                     /locus_tag="OO335_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6001217.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26453..28597
                     /gene="pta"
                     /locus_tag="OO335_000723"
     CDS             26453..28597
                     /gene="pta"
                     /locus_tag="OO335_000723"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6001218.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28787..30307
                     /gene="yfcC"
                     /locus_tag="OO335_000724"
     CDS             28787..30307
                     /gene="yfcC"
                     /locus_tag="OO335_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6001219.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OO335_000725"
     CDS             complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OO335_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6001220.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OO335_000726"
     CDS             complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OO335_000726"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6001221.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OO335_000727"
     CDS             complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OO335_000727"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6001222.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32327..32974
                     /gene="yfcG"
                     /locus_tag="OO335_000728"
     CDS             32327..32974
                     /gene="yfcG"
                     /locus_tag="OO335_000728"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6001223.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33031..33393
                     /gene="folX"
                     /locus_tag="OO335_000729"
     CDS             33031..33393
                     /gene="folX"
                     /locus_tag="OO335_000729"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6001224.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33414..34307
                     /locus_tag="OO335_000730"
     CDS             33414..34307
                     /locus_tag="OO335_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6001225.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OO335_000731"
     CDS             complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OO335_000731"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6001226.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OO335_000732"
     CDS             complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OO335_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6001227.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36223..36939)
                     /locus_tag="OO335_000733"
     CDS             complement(36223..36939)
                     /locus_tag="OO335_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6001228.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OO335_000734"
     CDS             complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OO335_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6001229.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OO335_000735"
     CDS             complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OO335_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6001230.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OO335_000736"
     CDS             complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OO335_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6001231.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OO335_000737"
     CDS             complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OO335_000737"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6001232.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OO335_000738"
     CDS             complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OO335_000738"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6001233.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OO335_000739"
     CDS             complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OO335_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6001234.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OO335_000740"
     CDS             complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OO335_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6001235.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OO335_000741"
     CDS             complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OO335_000741"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6001236.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OO335_000742"
     CDS             complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OO335_000742"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6001237.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45788..46447)
                     /locus_tag="OO335_000743"
     CDS             complement(45788..46447)
                     /locus_tag="OO335_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6001238.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OO335_000744"
     CDS             complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OO335_000744"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6001239.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47342..48355)
                     /locus_tag="OO335_000745"
     CDS             complement(47342..48355)
                     /locus_tag="OO335_000745"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6001240.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OO335_000746"
     CDS             complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OO335_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6001241.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49656..50651
                     /gene="flk"
                     /locus_tag="OO335_000747"
     CDS             49656..50651
                     /gene="flk"
                     /locus_tag="OO335_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6001242.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLTAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50648..51826)
                     /locus_tag="OO335_000748"
     CDS             complement(50648..51826)
                     /locus_tag="OO335_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6001243.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OO335_000749"
     CDS             complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OO335_000749"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6001244.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53481..55487
                     /gene="mnmC"
                     /locus_tag="OO335_000750"
     CDS             53481..55487
                     /gene="mnmC"
                     /locus_tag="OO335_000750"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6001245.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55608..55886)
                     /locus_tag="OO335_000751"
     CDS             complement(55608..55886)
                     /locus_tag="OO335_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6001246.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OO335_000752"
     CDS             complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OO335_000752"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6001247.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56468..57277)
                     /locus_tag="OO335_000753"
     CDS             complement(56468..57277)
                     /locus_tag="OO335_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6001248.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OO335_000754"
     CDS             complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OO335_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6001249.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OO335_000755"
     CDS             complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OO335_000755"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6001250.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OO335_000756"
     CDS             complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OO335_000756"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6001251.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60323..60874
                     /gene="smrB"
                     /locus_tag="OO335_000757"
     CDS             60323..60874
                     /gene="smrB"
                     /locus_tag="OO335_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6001252.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60978..61868)
                     /locus_tag="OO335_000758"
     CDS             complement(60978..61868)
                     /locus_tag="OO335_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6001253.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61855..62379)
                     /locus_tag="OO335_000759"
     CDS             complement(61855..62379)
                     /locus_tag="OO335_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6001254.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62376..62846)
                     /locus_tag="OO335_000760"
     CDS             complement(62376..62846)
                     /locus_tag="OO335_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6001255.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62843..>63060)
                     /locus_tag="OO335_000761"
     CDS             complement(62843..>63060)
                     /locus_tag="OO335_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6001256.1"
                     /translation="ETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLE
                     AVPVKQSGTTLAAAEFNASATIVVDYQ"
BASE COUNT        15776 a        17115 c        15716 g        14453 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaatgac
      601 gccttttatt gataatttac atatgaaaaa attttccttt tgttatatta tttctgtttt
      661 cactccctct gattattttt accaaccctg ggtcactgca ttatcgccaa cgccccgaca
      721 cgtaagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg
      781 taatgccggc aatattgata atctgaatag gtccgcagag tatataaaag agttctttgt
      841 cagtagcggt gccagagtta ccgcgcaggg cgtccccgat tggctattac gactctgcgc
      901 ctggaagcca gaattaccct tacccggcaa tgtcctggct ttatcccgat cggggagact
      961 ttattgctgt ggtcggcaga atgcaggata tcaacgccgt tcgtcaggta aaagcggcat
     1021 tgttgtcatc ccgggattta tctgtttatt ctatgaatgc cccagggttt attcccggta
     1081 ttgatttttc tgatcacctc aattattggc aacacgatat tcccgccgta atgattactg
     1141 acaccgcttt ttatcgtaat aaacaatacc acttgcccgg tgataccgca gacagattga
     1201 attatcagaa aatggctcag gtggtagatg gtgttataac cttgttatac aacagtaaat
     1261 aataaaaatt tctcgaggcg ggtccagaaa cagcgacatg tacattttat tttccctcgt
     1321 ctgttttaag atgcgaaaca taaaattcaa tggaggaaaa ataaatggga atgattggct
     1381 attttgcaga aattgactca gaaaagatta accaactgct tgagtctact gaaaaacctt
     1441 tgatggataa tatacatgac acgctttcag gattacgccg actggaatca ctggatcgaa
     1501 gtgagctgcg taaacagttt tccattaagc gactcaatga aatggagatt tatccgggag
     1561 tgaccttttc agaagagtta gaggggcaac tctttgcctc gatcatgctt gatatggaaa
     1621 agctgattag cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg
     1681 ttgggtgatg gtgtcagagc gccaaaaaaa accgccgaac atgtcggcgg ctttctgact
     1741 tacaaactaa cagtttacat cagcggcatt gctaaccgca caatactaat cagcggttgt
     1801 ggccatacac ccagcaccag taccaacagt gcagaaatca gcaccacgat accgcccgcg
     1861 ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt ccggtgcgtg caggtacagg
     1921 ctcaccgcca cgcgcaggta gtagtagagg ccgattgccg aaccgacaac cacggcaccc
     1981 accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt accgataaag
     2041 cccagcgtca tcgggatacc ggccagagac agcatcatca ccgtcatcac tgccgcgagg
     2101 atcggacgat gccagaacag accgcggtaa gagaacaggg aatcagcatc cgggccacga
     2161 tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctgaa cagataaccg
     2221 gccaggtaaa cccctaccgc ttccatcgac atctcgccgg tttgcagcgc aatcagcgcc
     2281 accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt gatgttggtc
     2341 tggctcaacg ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagtact
     2401 acgcgaatcg cttcgctgtc acccaccggt gcgtagagga acagacgcat caccacaccg
     2461 aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg
     2521 tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca
     2581 atcatcatgc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccgaggttt
     2641 ttacccagcg cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccgaac
     2701 agcaggaaag aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagcgaa
     2761 cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcaata
     2821 ccgaggaaca gagacgccag atggtttgca ttcgccagca ggatcccgcc cagcgcggca
     2881 attaacacca acaggtagaa ctcatccttg ttgtcgttat agccttcaag ccacgggtag
     2941 gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa
     3001 ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg cctggccaac aaaccagagc
     3061 gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt
     3121 cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc
     3181 agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg
     3241 gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc
     3301 tgcggataga agcccagcag taccagcagc accaccagca acaggatcat aaacagctca
     3361 cgcagcgaca tccctggcag ttcctggctg gcaatctggc ttttcgcttt accgaagtaa
     3421 gcgcgatgca acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata
     3481 acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag
     3541 ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc
     3601 catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca
     3661 taaagctgac cgcagagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg
     3721 attaccgcgc cctggtaggc caactggctg ccggtgtaga ttgcaatcag cacgaagccc
     3781 atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgagc gaaggtcatc
     3841 cacgcaccgt agaagatgcc gataacaccc agccacatag cgattggcgc gaactctgcc
     3901 gacgcgttcg ggaacagcgg cagggagaaa cgcaacaaac cgtaagcggc ggttttcagc
     3961 aagatccccg cgaggtccac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc
     4021 cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatc
     4081 aacagatatt ccacaccact ggacattggc gtattcagca gctcttcata gttgaaggtc
     4141 cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcgat caacatcacc
     4201 agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg
     4261 tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc
     4321 cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg
     4381 atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat
     4441 agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga cagaccgtca
     4501 atggcgagat gaatagagat accaaaacgc gggatccacg gcatgtcgaa ttcagactgc
     4561 cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc
     4621 gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca
     4681 aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt
     4741 agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat
     4801 tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcgccg cgaaggcagc
     4861 ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag
     4921 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     4981 cagataaccg ttctcgctta acagcagacc tttacctgca aagcgggaaa gaaccgccgg
     5041 gatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     5101 cttgacgaac actttgtcgt acagccagtc aaagccccaa gcgttgtacc accaggtact
     5161 cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc agagaacgtt tacccagcca
     5221 cagccaggct gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa tttccagggt
     5281 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     5341 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     5401 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     5461 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     5521 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
     5581 cagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat
     5641 agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac
     5701 ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat
     5761 cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt
     5821 agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag
     5881 cagcgtaacc gccccgacaa tacccaccag atgcagaact tccggcgtca tcaggaacag
     5941 gccgtgggta cgggcgatca ggtagacacc cgcggttacc atcgttgcgg cgtggatcag
     6001 cgcggagaca ggcgtcgggc ccgccatcgc gtcagcaagc catgtctgca acggcaactg
     6061 cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt
     6121 gccgtcagca aagtgcgctg gtgccagttc caccatatcg cggaagttca gggtgcccag
     6181 ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac
     6241 gaacgctttc attgctgctg cgccattctt cggatcggtg taatagaaac cgatcagcag
     6301 ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt tatcggcgag
     6361 aaccagaact accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc
     6421 ctcttcaccg cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac
     6481 caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc
     6541 gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa
     6601 atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat
     6661 cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa
     6721 tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg
     6781 ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct
     6841 gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag
     6901 cttccggcga ccacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc
     6961 ataaacagca gattgcgacg gataaccaga ccggttaagc caagaacgaa taagattgcc
     7021 gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc
     7081 tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg
     7141 ccacaaccag acccgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc
     7201 cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt
     7261 taacaccgag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccgggc
     7321 caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt
     7381 tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt
     7441 ccagcgcacc ggcgaagtaa gcgcctagtg agaagaacac cccggaaatc gccagcagcg
     7501 aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg
     7561 caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg
     7621 taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc
     7681 gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga
     7741 gatcagcaga tcctctttct cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa
     7801 atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat
     7861 gcagcgtgag aagttaatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc
     7921 tttttgcagc gagatacagc cgaccgggca ggctaccgca cagaggttac aggctacgca
     7981 acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag
     8041 atagaccggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat
     8101 catccagata ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt
     8161 cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc
     8221 agcgtcagcg gcaggcagat tttccagccg aaggacatta cccggtcata acgcggacgc
     8281 ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac
     8341 cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat
     8401 gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg
     8461 gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg
     8521 tgacggtgac acaccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg
     8581 atgacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc
     8641 tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca
     8701 gaagcacgca tcgcacccag cagcgagtat ttgttgttac ttgaccagcc cgcaaacagc
     8761 accgcgtaaa ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg
     8821 gcaaccaccc aacccggact gactggcaca attgcaaagg ccagcagcag cgaggtaaag
     8881 gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct
     8941 ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg
     9001 ttaggtccat aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat
     9061 gccccacagg tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc
     9121 tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg
     9181 cgccagccag caccggagca atgccggaca tacccatcgg caagcccacc tgccctgccg
     9241 tcagtccttc ggcgatttca accggcagcg tgaccgtgtt gccatcgtaa ctaaaggaga
     9301 cgcgtgtacc tgcgttcaca cccaacttcg cggcatccgc tgggttgagt ttgatgtacg
     9361 gctgcggcat acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca
     9421 ggtggtagta cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc
     9481 tggtgaagta atccagaccg ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa
     9541 agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc
     9601 ccggcgccca ggcaaacggc acttgcgaac ggtgcgcggt cggctggttg ttaccttcca
     9661 tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat
     9721 tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac
     9781 gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc cgccagttct gggattttcg
     9841 ccacaacagc gtcaattaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaagg
     9901 tgctgtgcag cgagtgcagc cagcgccagc tttccagcat gacagttttg ctgtcgtaat
     9961 aagccggatc gtaaacctgg aagaaacgtt gggcgcgacc ttcattatta attaccgtac
    10021 cgtcgctttc agcaaagctg gcagaggaaa gcaccagatg ggcgttttcc atgatcgctg
    10081 tgcgttgatg atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg
    10141 tagcagaagc gtaacgatgc agatcgtttt ccaacaccac caccgcgtcg gcgcgtccgg
    10201 tttccagttc ggttaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc
    10261 tgttgacgga acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt
    10321 tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa
    10381 tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg ttttgcagct
    10441 caggttcgat accgtcaacc gctggtgcgg agttatccag cgcatgggcg atggcaaaac
    10501 ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac
    10561 gagtgtcatc aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga
    10621 ttgccgcaat ctgccagtca gccactttct gtgctgcagc catttcgcgc gctttacctt
    10681 tcaccgcctg acgcactgcc agtgcgacgc gcgcgccggt ctgggtaacg tcttcgccca
    10741 gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt
    10801 cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt
    10861 agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg
    10921 gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg
    10981 cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac
    11041 gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt
    11101 ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc
    11161 tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt
    11221 tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga
    11281 ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt
    11341 tgtcgtgcgc gccataaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca
    11401 cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct
    11461 gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacgg
    11521 tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt
    11581 tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg
    11641 aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt
    11701 cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg
    11761 ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt
    11821 tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc
    11881 ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat tcggttacca gcgctctttc
    11941 agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc
    12001 gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt
    12061 gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg
    12121 atatcgcccg gctggccttc gccacgctcc agcgcacgca gaattttcac gctccacggc
    12181 agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg
    12241 ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc
    12301 agacggctgc ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg
    12361 gtcaggaagt cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc
    12421 ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac
    12481 agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt
    12541 ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg
    12601 gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg
    12661 ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc
    12721 ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc
    12781 ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg gcgcagattt
    12841 actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca
    12901 aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg
    12961 tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg
    13021 gattcgtctt tcggcatcag gctccacttc aggccagtcg aaaagcccgc gccgccgcgc
    13081 cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc
    13141 gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt
    13201 ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc
    13261 atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct
    13321 catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg
    13381 taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg
    13441 cctgaatacc ctgatacccg ttgatatgac agaccacgct gtcgcaataa cggatcacat
    13501 ggcgaccaac cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc
    13561 ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac
    13621 gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt
    13681 gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg
    13741 gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc
    13801 cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa
    13861 atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt
    13921 caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg
    13981 catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag
    14041 cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg
    14101 catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag
    14161 cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt
    14221 ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag
    14281 gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg
    14341 ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt
    14401 cggcatccag tcgaggaact cacgcagcag acgatcccag ccgcgcggca ggtcgtgcgc
    14461 tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag
    14521 atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc
    14581 ctgaataaag gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat
    14641 aacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag
    14701 cacgtaaggc atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat
    14761 gtagctgtgc caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat
    14821 gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc
    14881 gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcat tttcggtgcc
    14941 gcgcttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt
    15001 ggctttggtc agctcaaacg gcgagaattc ggtagcgcgt gccgggtaat ctttacgcag
    15061 cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa
    15121 agtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt
    15181 ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg
    15241 gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc
    15301 gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt
    15361 cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac
    15421 gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag
    15481 ttcgccaatt accggatcat caagatgatc gcgggtctgc caggcgggtt cttgcgcggt
    15541 taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc
    15601 ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta
    15661 aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg
    15721 ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg
    15781 acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg
    15841 cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac
    15901 ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt
    15961 tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt
    16021 tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa
    16081 acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag
    16141 accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt
    16201 gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt
    16261 ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat
    16321 cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg
    16381 ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag
    16441 acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc
    16501 gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa
    16561 catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat
    16621 accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc
    16681 gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca
    16741 gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc
    16801 gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa
    16861 aaaaacctta caataactgt agaaattctt ttatacagct aattgatgtg gtcttttact
    16921 cctttctata accttttgtc aactttaaca aaagcttctt cacattagtt tacataatat
    16981 caacaccatt agcatttaat gccctttcac cccagatcct tgacgactcc aggataatta
    17041 gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag
    17101 gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt
    17161 gttattccat aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc attgtagcag
    17221 gtgatttttt tcaggcgatt atttgtgcat tcgggacgtg aatctctggt gattgaaaaa
    17281 taaacagttt tgtacgttct gcactatgcg aggaaggatt tttactaaaa aaaagccgct
    17341 ggggtttaaa acacccccag cggctcgttt tttacactat tgtctcagga attatctatc
    17401 gtccgtcgat tactcgatat ccctttcaat caacaatgaa tcatcccctt ccggagcaga
    17461 cattgggctg tattgccacg gattatggta gctctccatc gcctgataga tcacctgggc
    17521 cagctcatta ttactggacg ggtcatagca cagcaaatat tcggtatcag gtagcggcgg
    17581 taagccatct actccactca gcacacgcag gtccgggctc atcatctcaa ccggccttgc
    17641 cgtcacgcca agaccggctt tcactgccgc acgaacggcc ggaagcgtcg aggcgacata
    17701 agccagtcgc catggaatat ctactttatt aagcgtcgcc agcaccatat cgcgaaacgg
    17761 acttggatca tccagcaata caagagggat cggctcgcct ttttgcaata cgtattccgc
    17821 tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat gccttaaacg ctgaaggacg
    17881 atgggtggtt accatcaaat ccacttcctg ggattccagc atttctgcca tataggcatt
    17941 acgtttaacg cggacatcca gcgctaattt cggataaacc gaactcacgc gatttaacag
    18001 gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg atagttaaca cgccctgaag
    18061 attactgaac attaatgatg agcaggcctc atcattaaaa cgcaggattt tcctggcgta
    18121 accaagaagt tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag
    18181 ttctttccca acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg
    18241 acacacagcg gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct
    18301 cagcagatcg aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt
    18361 actggcggct catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg
    18421 taatgtgcct ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt
    18481 tgcactaaaa aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat
    18541 atcttcaggg atcgcatatc tattaagtta ctcactcttt tctatttatg acatgcgcgt
    18601 gtttgtataa atgtaaatgt gagtccttgt tccactctcg tgcagcatcg ctggtcatac
    18661 gcgaacacgt acaaacagca atggtgaagc ggcatcaaga gcagggatcc gtcatttatg
    18721 cgagcatttt accccaaaaa ccttttattt ataagggtca ttgcgaatta tctgatgcaa
    18781 agttatgtta tgttaggcaa agtaatcttc tattattaat aagcacatca aaaccttttt
    18841 gaatattaaa taataattaa ttagcatcat cctcattcat taattccgct taacaatagt
    18901 ttcacacctt acccccattg cgacacctcg gaaatcatca cgctgttaaa ctctccgtaa
    18961 tgcggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat tttcacacaa
    19021 ttatcttaca gataaaaaaa ccagacttgc aattaagaat tagaacaatt cactatataa
    19081 cattgcatgt aaagcatata agcctcatta tttgtcatta ttaagttatt aacagcacaa
    19141 tcgagccttc ccctctggca aaatcttatt ctgcagacct tcaaaacacc gtcctggggg
    19201 agtacattgt tctaagctga cttccacggc agggagtggc gataacagca aaaaaggtca
    19261 agattcatgt cccccattga aaaatccagc aaattagaga atgtctgtta tgacatccgt
    19321 ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag gtaacaaggt actgaaactg
    19381 aacatcggca acccagcccc attcggtttt gacgcgccag atgaaatcct cgttgacgtg
    19441 atacgcaacc tgcctaccgc tcaagggtat tgcgattcca aagggcttta ctccgcgcgt
    19501 aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg atgttaccgt ggaagatatt
    19561 tacatcggca atggtgtatc ggagcttatc gttcaggcaa tgcaggcatt gctgaacagc
    19621 ggggacgaaa tgttggttcc tgcaccagat tacccactct ggaccgcggc ggtttcgctt
    19681 tccagcggta aagcggtgca ttatctttgc gatgaatcct ctgactggtt cccggacctc
    19741 gatgatattc gcgctaaaat tacgcctcgt acgcgtggga ttgttattat caacccaaat
    19801 aacccaaccg gcgcggtata ttccaaagag cttttaatgg agattgtgga gattgcacgt
    19861 cagcataatc tcattatctt cgccgatgaa atttatgaca aaattctcta cgacgatgct
    19921 gagcatcact caattgcgcc gctggcacct gacctgctga ccattacctt taacggactg
    19981 tcgaaaacgt accgcgttgc aggcttccgt caggggtgga tggtgttgaa cgggccgaaa
    20041 aaacacgcca aaggctacat cgaaggtctg gaaatgctgg cttcaatgcg cctgtgtgct
    20101 aacgttcctg cgcaacacgc cattcagacc gcgctgggtg gttatcagag catcagtgaa
    20161 tttattaccc ctggcggtcg tctttatgag cagcgtaacc gcgcgtggga actgatcaac
    20221 gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc tgtatatgtt cccgaaaatc
    20281 gacgccaaac gctttaacat tcatgacgat cagaaaatgg tgctggattt cctgttgcag
    20341 gaaaaagttc tgttggtgca agggacggca ttcaactggc cgtggccgga tcacttccgc
    20401 attgtcacgc taccgcgtgt cgatgatatc gagctgtctt tgagcaagtt cgcgcgtttc
    20461 ctttctggtt atcatcagct gtaatcttaa tttcactgcc ggagattgca tccggcagtg
    20521 ttatcccgcc acaatgacct gatgatgtca tcatacgtaa ggtcactatg aaacagagcc
    20581 atttttttgc ccatctctcc cgcctgaaac tcattaaccg ctggccgcta atgcgcaacg
    20641 tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc gatggtcgcc catgcgctgg
    20701 cagctatcaa aaatcggaaa tttggcggta atgtcaacgc cgaacgtatc gctttactgg
    20761 cgatgtacca cgatgcctca gaagtgctca ccggcgatct ccctactccg gtgaaatact
    20821 tcaatgcgca aatcgctcag gagtacaagg ctattgaaaa aatcgctcag cagaaactgg
    20881 tcgatatggt tccggaagaa ctgcgggata tctttgcgcc gttaattgac gagcatgcat
    20941 atagcgatga agaaaaatcg ctggtgaaac aggcagatgc actgtgtgca tatctgaaat
    21001 gtctggaaga actcgcggcc ggaaataatg aattcttgct ggcaaaaacg cgactggaag
    21061 cgacgcttga agcgcgtcgc agccaggaga tggactactt catggaagta tttgttccca
    21121 gcttccatct ttcgctcgat gagattagcc aggattcacc gctgtaagca gccggagtct
    21181 gcgtcgcatc agtcaataag cgccggatgc gacatcaggc tcttgtcaaa acggaaacag
    21241 catcgggatc atcaccacac aaaccgccat cacgataatg gtgaacggta cccccaactt
    21301 cacaaagtca ctgaagctgt aatttcccgg acctaaaacc agggtgttaa caggtgaaga
    21361 caccggtgtc ataaaggcgg cggatgctgc catcgccacg accatcgcaa atggataagg
    21421 cgacactccc atcgttttgg cagcagccag cgcaatcgga gccatcaaca ccgccgtcgc
    21481 ggtattagag ataaacagcc caataaccgc cgacaagaca aacaaacacc ccagcatcat
    21541 atgtggccca taaccgccgc caatatccat cagccctttc accgccagcg cgacacctcc
    21601 cgttttctgt aatgccacag caaacggcat catcccaacg atcaaaataa tgctcggcca
    21661 gtgaatggat ttataggcgc tttcagcatc tatacagcgg aatttcccca tcagcaggca
    21721 ggcgatgata gcggcaacag gattaggaat ttcatttgtc agcattaacg ccaccattag
    21781 caccagacag aaaatggcat ggggtgcctg gctgtgcgcg ggcgatgctt cactcacctc
    21841 ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg gccagcatac cgatcagttt
    21901 ccagttaccc acaaccagga tgatatcgcc cagcagcaga ggctcatccg ccagcgaacc
    21961 ttccagcgcc acgccattgc gcttgagccc caccacattc agtccgtagc gggtacgaaa
    22021 accaatttcg cgcaccgatt taccaatcag ttctgactca ggaattaatg aaatctctgc
    22081 catgcccaca tcaagggcct ggtcagaaaa atactcgccg cgcagtacca tcggctccag
    22141 caattgctca ctacaaaatt gccggagatc gacatcagcc gcagacatat caataagcaa
    22201 aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc acgataacgc gacgaaaacg
    22261 ccgccagcgt tcaacaccga tgacgttagc gccataacgc tcacgtaatt tgagatcatc
    22321 cagccgttga cctatcattg gcgatccggg gcgaatagcc agacgtcgcg cacgcccggt
    22381 cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc gtccagcctt cgcgctgcgg
    22441 ggtctgggta tcccctttca gcatgaaacg catcactaac atatacaaaa tacccagcac
    22501 cagcacgacc aggccaatag gtgttacgct aaagaaactg aagccgtgat agccttcacg
    22561 cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc accagcgtca tcatgccgct
    22621 aatcagcccg gcaaaactta acggcatcat cagacgtgac ggcgaagttt gcatacgcat
    22681 ggcaacgctt aacaccacgg gaataaagat agcgacaacg ctggttgaac tcataaacgc
    22741 gccaagcccc gcgacggtca gcatcaacaa aaccaacatt ttgatttcac tgttgcccgc
    22801 cactttgacc agccatgttc ccattacggt ggcaacaccg gtacggacca aaccatcgcc
    22861 aataataaac aaggcggcaa tcaggacaac gttaggatca gaaaagccgg aaaatacttc
    22921 ggggaccgtc agcgttccgc ttaatgcaaa cgcgacaata acaaacaaag cgaccgcatc
    22981 catacgcact ctgcccgtcg caaacaagac gatggcaacg gccagtaatg aaagaaccca
    23041 aatcaattca ccgttcacaa cttatccttg ttaattgagg gggatgactt gattctgcca
    23101 taaaaaagcc ccgacgagac ggggctaaat catgatcagg tgtttcactg aataataaca
    23161 tcgccatttg gctgtttggt cacagtaatt tgctccagac tgtggaggac caaatcgacc
    23221 tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa tgacatggca acccgccgcc
    23281 aggccagaaa gcacgccagc gggagcatct tccaccacca cacactcctg cggcgcaagc
    23341 cccagcagct gcgcacctaa cagatacgca tcaggttctg gttttccgcg cttcactcgc
    23401 tcagcggtta caaacacctc tggtgcggga agccccgcta ttttatggcg cgctcgcgct
    23461 accggcatgg agccagaagt cacaatggcc cacggaatac ctgctttatt caaatgattg
    23521 agtaaggcga ttgccccagg aagcgcggta ataccttcgg tttccgtggc ctcgatttgc
    23581 tccagacgcg taaactcggc ggcaatatca gcctcggatt tgcccgccat aaaatggcgc
    23641 aaagaggtga tcgcctgttt accgtgaatg aaagccagca cctcttccgg cgctaaccca
    23701 tgacgtctgg cccagttgct ccacgcccgt tctaccgcag gcagggaatc caccagcgtt
    23761 ccatcaagat caaacagaaa acctttgcac cgcacgcggg cctcctcagg cgttgataat
    23821 ttgattaatt tcgttggcgc tcaaatggta ctgacgcggg caggcatgcc acacattaag
    23881 catgcgctga tatttttccc acattggcgt ctgggcgtta aaaccatgag tcccagcgtc
    23941 aaaatgggta tagcgccctt ccacattaac cataaagcgg acataaccga ggtaacgtgc
    24001 ttcagtggcg gcgtcaaagc cgaggaaggt gacacgacgt tcatcgatgg attgctggtc
    24061 ctgcaaatta gaccaagaaa catacaacgc atgatacata tccataatgt cgatgatagt
    24121 gcggcaggtt tcttctttca gctcgccaaa ctcgcgatcc aattcacgca tctgtaatcc
    24181 gtaaccacgc tcaataattg tttgcaggcg acggtaacgt tcagcatttg ccggatcgag
    24241 catagtcatc atcttgtact gattagacaa aataagacgt tgcgcgttgg tcatttccat
    24301 tgttgactcc tgtatcactc tactacggtg aaaaaaaaga aggctgagta tgccttcttt
    24361 tatatgcgta atcaggggtc aattacaaat catcaaggaa agttttatcc agttgtttga
    24421 aggcgcgctt aagcgtgtca gctaatgcct ggtaatcagg cttgccttca acgggtgcca
    24481 acacctgtcc agactcctgc aatttaccgc gaacttcata aaaccagtta aggattgcag
    24541 ggggtaatgg cgttacagaa cgcttgccca gccaccacaa tccctgcatg ggtaaactta
    24601 aggcgaacag tgcagtggca actgccggcc caagcagacc gcccagggca atctgccagc
    24661 agagagtaaa tacggcgatc ggcggcataa aacggatcgc ataacgcgtc atcttgataa
    24721 cgcgattttc gacaaagacc ggagcaaggc gtttttccag tggccacgtc tttgagtaat
    24781 gctgtccgcg gcgaaacaag ctaaaaaaat taacagaacg attatccggc gttgacatgc
    24841 ttcacctcaa cttcacatat aaagattcaa aaatttgtgc aaattcacaa ctcagcggga
    24901 caacgttcaa aacattttgt cttccatacc cactatcagg tatcctttag cagcctgaag
    24961 gcctaagtag tacatattca ttgagtcgtc aaattcatat acattatgcc attggctgaa
    25021 aattacgcaa aatggcatag actcaagata tttcttccat catgcaaaaa aaatttgcag
    25081 tgcatgatgt taatcataaa tgtcggtgtc atcatgcgct acgctctatg gctccctgac
    25141 gtttttttag ccacgtatca attataggta cttccatgtc gagtaagtta gtactggttc
    25201 tgaactgcgg tagttcttca ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt
    25261 acctttctgg tttagccgaa tgtttccacc tgcccgaagc acgtatcaaa tggaaaatgg
    25321 acggcaataa acaggaagcg gctttaggtg caggcgccgc tcacagcgaa gcgctcaact
    25381 ttatcgttaa tactattctg gcacaaaaac cagaactgtc tgcgcagctg actgctatcg
    25441 gtcaccgtat cgtacacggc ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg
    25501 ttattcaggg tatcaaagat gcagcttctt ttgcaccgct gcacaacccg gctcacctga
    25561 tcggtatcga agaagctctg aaatctttcc cacagctgaa agacaaaaac gttgctgtat
    25621 ttgacaccgc gttccaccag actatgccgg aagagtctta cctctacgcc ctgccgtaca
    25681 acctgtacaa agagcacggc atccgtcgtt acggcgcgca cggcaccagc cacttctatg
    25741 taacccagga agcggcaaaa atgctgaaca aaccggtaga agaactgaac atcatcacct
    25801 gccacctggg caacggtggt tccgtttctg ctatccgcaa cggtaaatgc gttgacacct
    25861 ctatgggtct gaccccgctg gaaggtctgg tcatgggtac ccgttctggt gatatcgatc
    25921 cggcgatcat cttccacctg cacgacaccc tgggcatgag cgttgacgca atcaacaaac
    25981 tgctgaccaa agagtctggc ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg
    26041 ttgaagacaa ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt tactgccacc
    26101 gcctggcgaa atacatcggt gcctacactg cgctgatgga tggtcgtctg gacgctgttg
    26161 cattcactgg tggtatcggt gaaaatgccg cgatggttcg tgaactgtct ctgggcaaac
    26221 tgggcgtgct gggctttgaa gttgatcatg aacgcaacct ggctgcacgt ttcggcaaat
    26281 ctggtttcat caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac
    26341 tggttatcgc gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc agctcagctg
    26401 gcggtgctgt tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg
    26461 tattattatg ctgatcccta ccggaaccag cgtcggtctg accagcgtca gccttggcgt
    26521 gatccgtgca atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc
    26581 gcgtaccggt ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga actcttccac
    26641 cacgacggcc gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca
    26701 gaaagatgtg ctgatggaag agatcgtcgc aaactaccac gctaacacca aagacgctga
    26761 agtcgttctg gttgaaggtc tggtcccgac acgtaagcac cagtttgccc agtctctgaa
    26821 ctacgaaatc gctaaaacgc tgaatgcgga aatcgtcttc gttatgtctc agggcactga
    26881 caccccggaa cagctgaaag agcgtatcga actgacccgc aacagcttcg gtggtgccaa
    26941 aaacaccaac atcaccggcg ttatcgttaa caaactgaac gcaccggttg atgaacaggg
    27001 tcgtactcgc ccggatctgt ccgagatttt cgacgactct tccaaagcta aagtaaacaa
    27061 tgttgatccg gcgaagctgc aagaatctag cccgctgccg gttctcggcg ctgtgccgtg
    27121 gagctttgac ctgatcgcga ctcgtgcgat cgatatggct cgccacctga atgcgaccat
    27181 catcaacgaa ggcgacatca atactcgccg cgttaaatcc gtcactttct gcgcacgcag
    27241 cattccgcac atgctggagc acttccgtgc cggttctctg ctggtgactt ccgcagaccg
    27301 tcctgacgtg ctggtggccg cttgcctggc agccatgaac ggcgtagaaa tcggtgccct
    27361 gctgctgact ggcggctacg aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt
    27421 cgctaccggc ctgccggtat ttatggtgaa caccaacacc tggcagacct ctctgagcct
    27481 gcagagcttc aacctggaag ttccggttga cgatcacgaa cgtatcgaga aagttcagga
    27541 atacgttgct aactacatca acgctgactg gatcgaatct ctgactgcca cttctgagcg
    27601 cagccgtcgt ctgtctccgc cagcgttccg ttatcagctg actgaactgg cgcgcaaagc
    27661 gggcaaacgt atcgttctgc cggaaggtga cgaaccgcgt accgttaaag cagccgctat
    27721 ctgtgctgaa cgtggtatcg caacttgcgt actgctgggt aatccggcag agatcaaccg
    27781 tgttgcagcg tctcagggtg tagaactagg tgcaggcatt gaaatcgttg atccagaagt
    27841 ggttcgcgaa agctatgttg gtcgtctggt cgagctgcgt aagaacaaag gcatgaccga
    27901 aaccgttgcc cgcgaacagc tggaagacaa cgtggtgctc ggtacgctga tgctggaaca
    27961 ggatgaagtt gatggtctgg tttccggtgc tgttcacact accgcaaaca ccatccgtcc
    28021 gccgctgcag ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg tgttcttcat
    28081 gctgctgccg gaacaggttt acgtttacgg tgactgtgcg atcaacccgg atccgaccgc
    28141 tgaacagctg gcagaaatcg cgattcagtc cgctgattcc gctgcggcct tcggtatcga
    28201 accgcgcgtt gctatgctct cctactccac cggtacttct ggtgcaggta gcgacgtaga
    28261 aaaagttcgc gaagcaactc gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg
    28321 tccgctgcag tacgacgctg cggtaatggc tgacgttgcg aaatccaaag cgccgaactc
    28381 tccggttgca ggtcgcgcta ccgtgttcat cttcccggat ctgaacaccg gtaacaccac
    28441 ctacaaagcg gtacagcgtt ctgccgacct gatctccatc gggccgatgc tgcagggtat
    28501 gcgcaaaccg gttaacgacc tgtcccgtgg cgcactggtt gacgatatcg tctacaccat
    28561 cgcgctgact gcgattcagt ctgcacagca gcagtaatct cgtcatcatc cacagctttg
    28621 cgctgcggat atctgaaccg gaaataatca ctatttccgg ttttttattc tcttaatttg
    28681 cattaatcct ttctgattat cttgcttaac tgcgctgcat caatgaattg cgccatctca
    28741 ctttgcatac ttaccacttt gttttgtgca agggaatatt tgcgctatgt ccgcaatcac
    28801 tgaatccaaa ccaacaagaa gatgggcaat gcccgatacg ttggtgatta tcttttttgt
    28861 tgctatttta accagtcttg ccacctgggt agttccggtg gggatgtttg acagtcagga
    28921 agtgcagtat caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt
    28981 tcgcattctg actaacgaag caggcgaacc tgagtatcac cgcgtacagc tgttcacgac
    29041 gggcgatgaa cgcccgggcc tgatgaactt cccgtttgaa gggttaacct caggatcgaa
    29101 atacgggaca gccgttggca tcatcatgtt tatgctggtg attggcggcg cgtttggcat
    29161 tgtgatgcgt acaggaacca ttgataacgg tatcctggcg cttattcgcc atacccgcgg
    29221 gaatgaaatt ctctttattc ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt
    29281 tggtatggga gaagaggccg tcgcctttgc cattatcatc gcaccgctaa tggtccggct
    29341 gggctatgac agtattacca ccgtcctggt gacttatatt gccacgcaaa tcggttttgc
    29401 cagttcgtgg atgaacccgt tttgtgtggt tgttgctcag gggattgccg gcgttccggt
    29461 gctttctggc tccgggttgc gcatcgtggt gtgggttatc gccactctga ttggcctgat
    29521 ctttaccatg gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga
    29581 gtccgaccgc ttctttcgtg aaaagcaggc ggatgttgaa caacgtccgt ttacctttgg
    29641 tgactggctg gtattgattg tcctgacagc cgtaatggtc tgggtgattt ggggcgtgat
    29701 cgttaatgcc tggtttattc cagaaattgc cagccagttc ttcaccatgg gtctggtgat
    29761 tggcatcatc ggcgttgttt tccgccttaa cggcatgacg gttaatacca tggcttcatc
    29821 ctttaccgaa ggggcgcgaa tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg
    29881 gattttgctg ctggttggta atggtgaagc aggtaatgcc agcgtgttaa atactatcct
    29941 caacagcatt gccaatgcca ttagcggtct ggacaacgcg gtcgcggcct ggtttatgtt
    30001 gctcttccag gctgtattta atttcttcgt gacgtccggt tctggtcagg cggcattaac
    30061 tatgccgtta ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct
    30121 ggcgttccag tttggtgatg gcttcagcca catcatttac ccaacctcag cttcgttaat
    30181 ggcaacgctc ggtgtgtgcc gggtggactt ccgtaactgg ctgaaggtgg gcgcgaccct
    30241 gcttgggctg ctgtttatta tgtccagcgt cgtagtgatc ggcgctcagt tgatgggcta
    30301 ccactaaaaa tgttaagagc cgcaattgcg gctctttttt cattctgccg tttcagtctc
    30361 tacggcttca tttttggcat tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg
    30421 agtgaactca tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa ctccactgac
    30481 ttcatcttcc tgtagggcga agggaccgtg agagacgcaa ctgaacaacg cgccccagac
    30541 acggcaattt ttatcttcga aatagaactg cccgtgctcg gcaaagggga caccggcaat
    30601 gcccaactct tcttccgctt cgcgacgcgc ggattccagc agttgctcat cggcctgaac
    30661 tacaccgcct gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg
    30721 ctggaccaga attttgccca tgccatcatg cacgacgatg taagttgcac gatgacgcag
    30781 acactgtgcc cgcatttgtt cccggctggc ttgtgcaatg acttcgttct cttcattgac
    30841 aatatccacc cattcagtac ttgccaaacg acgctgttcc attatcagga aaccttcttt
    30901 ttctggcgct cttacggcgc attttgagtt gtgggtaaat tacggattaa tcgcgacctg
    30961 cgcaatgata ctttgatcat tgagtgcgat tacactaagt acgtcattat ccagcatgcc
    31021 ataacccgcc ggattaccgc ctttcggaat actcaccgag ccggggttga agtggaaaat
    31081 ctccccccgc tgttctgcca ctggcagatg ggtatgaccg tacaccagca catcgttctg
    31141 gtttaaagca ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg
    31201 tttttccagt aatacctgtt gccacggcgc ggttatcggg aaatgcagca tcatttgatc
    31261 cacttcgctg tcgcagttgc cacgcacagc gataacctta tgtgccactt cattaagccg
    31321 ttcagcgact ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa
    31381 cacgtcgcca aggatcacca gccactgggc accgctttgg acaaacaact ccacaacacg
    31441 ttccgtcgcc ggtaacgacc catgaatgtc cgatgcaaac atcagtttca tcactcactc
    31501 ctcgtcgaaa aaacgtaagc tatgatactg gattctgtcg ttgctatcag ccagattgct
    31561 tcgccgaaag tgcaataaaa cgctggacag acgctcgctg ccactggaat gtcgcataat
    31621 ccaccaggcg ttctggcacc tcatcgccat gtagtaccag gcggttaatc attagcgcca
    31681 gatcagtatc agcaatgcac cattcaccaa ataaattcgg ctgacccagt gctaacaaat
    31741 gttctgccat cgcgaacagt ttctctgcac tggcttttcc ctcggccgtt agtggcgctt
    31801 ttttcgcccc cgcaaagaca acatccgtcg gacgctcttc gcggatgggc atcagatcgc
    31861 tgcgcagcca ggcctgaatc tgtcgtgcac gcgcacgatt ttctaaatca agcggataaa
    31921 tacgttccca ggtcggtggc gcaaatcgat cttccagata ctccgcaatg gcagaagatt
    31981 cactcaactc aaaatcatcg atttgtaata acggcacacg gcgtgtctga ccgtaacctt
    32041 gccacgtcgg ctgcaaatgt tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca
    32101 ggcctttttc ctgcaacgcc acccaggcgg ataacacata aggggagaaa aagtgggcat
    32161 ctgaccaaag cgtgatagcg ggtttactca ttatgtcctc ggcaataaag ggggctggct
    32221 atcaaactat agagcctttt tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg
    32281 ctttctatac ttgtggtgtt cagatgatta aacaaccgga gctgcaatga tcgatctcta
    32341 tttcgccccg acacccaatg gtcacaaaat tacgctgttt ctcgaagaag cagagcttga
    32401 ttatcgcttg attaaggtag acttggggaa aggcggtcag tttcgtccgg aatttttgcg
    32461 catttcgccc aacaacaaaa ttccggcaat tgttgatcat tctcctgccg atggcggcga
    32521 accgttaagc ctctttgagg ctgctgccat tttgttgtat ctggctgaga aaacaggact
    32581 ctttttgagt catgaaacgc gtgagcgcgc cgccacatta cagtggttat tctggcaggt
    32641 aggcggactg gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac
    32701 cattccttac gctattgaac gttatcaggt tgaaactcag cgtctttacc atgtactaaa
    32761 caagcggctg gaaaactcgc cctggctggg aggcgagaac tacagcattg cggatattgc
    32821 ctgctggccg tgggttaatg cctggactcg cctgcgaatt gacctcgcaa tgtatccggc
    32881 agtcaagaac tggcatgagc ggatccgttc gcgccctgcc accgggcagg cactgctaaa
    32941 agcacaactc ggtgatgagc gttcggatag ttaacagaaa caggttctcg tgtattattt
    33001 catcctaagt aaaacaacgg agaacctgca atggcacaac ctgccgctat tattcgtata
    33061 aagaaccttc gtttgcgtac gtttatcgga attaaggaag aagaaattaa caaccgtcag
    33121 gatattgtta tcaatgtgac gatccactac cccgccgata aagcgcgcac tagcgaagat
    33181 atcaacgatg cgctgaatta tcgcaccgta acgaaaaaca ttattcagca tgtagagaat
    33241 aaccgtttct ctttgctgga aaaattaact caggatgtgc tcgatatcgc acgtgaacat
    33301 cactgggtga cgtatgctga agtggagatc gataaactgc acgcgctgcg ctacgccgat
    33361 tcggtatcca tgaccttaag ctggcagcgt taatcgccat atcgggaggc tgcatgaaca
    33421 tagtgatcac cggagggacg ggattaattg gtcgccattt gattccgcgt ttgctggagc
    33481 tgggccatca aattacggta gtgacgcgta acccgcagaa agccagttcc gttctcggcc
    33541 ctcgggtgac actatggcaa gggcttgccg atcaaagcaa cctcaacggc gttgatgcgg
    33601 taatcaacct ggccggagaa ccgattgctg ataaacgctg gactcacaag caaaaagagc
    33661 gtctctgcca aagccgctgg aatatcacgc aaaaactggt cgatttgatt aatgctagcg
    33721 acacgccgcc gtcggtactc atttccggct cggcaacggg ctattatggc gacttaggtg
    33781 aagtggtggt taccgaagag gaaccgccgc ataacgaatt tactcataaa ctctgcgccc
    33841 gctgggaaga gattgcctgc cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgaa
    33901 ccggcgtggt actggcaccg gacggcggta ttctcggtaa aatgctgcca ccgtttcgtc
    33961 ttggcctggg cgggccgatt ggttccggtc ggcagtatct ggcctggatt catattgatg
    34021 atatggtcaa cggcattctc tggctgctgg ataacgagct gcgcgggcca tttaatatgg
    34081 tttcgcccta cccggtacgc aatgaacaat ttgcccatgc gctcggtcat gcgctgcatc
    34141 gcccggctat tttgcgcgtc cccacaaccg ccattcggct gttaatgggc gaatcttcag
    34201 tactggtatt aggtggacaa cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt
    34261 ttcgctggta cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt ggtttacagc
    34321 aaacatccgc cagttaactc cctgtgttac aggattagtg gctttgcgcg ataagatcgt
    34381 ctggtgaaag tcgggtcacc atcataacta actctctgtc taaacctcta tccagcatct
    34441 cctgagcaat acgcagggct tcttcgtgtt tgccctgcat tgcgccttct tcacgtaatc
    34501 tgtcagcaat ggtcatcagt ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa
    34561 taaatgcacg gaaacgctgg gcatcccctg tttgtaatac gtaattaaac agggctttta
    34621 gctgtctgtc attagtgttc cctgtaacta gcagcgaaac aatttggtcg actaatccca
    34681 acagatcgcg ctgacgaata tgtttctgaa ttaactccaa cagcgccatt ttgcggtgtt
    34741 gcataatctc gtcatccggc accacggtaa tatccaccaa cggaaacgct gatgaatata
    34801 ttttgcgggc tatagcaggc tcggcaaatt catcaagcca gcagagtgaa taaggataag
    34861 gacttctgca accatgataa aacagcatcg ggatcaccaa tggaagctct ttatagcccg
    34921 catcaagatg gttttgcatt gccgcaatgg aataacgcat catgcgaaaa gccattaatt
    34981 cttccggctt actttggtgc tctatcacta cataaatata acccactccc tcctgcgttt
    35041 tcacagacca caagaggtcg gaataatatt gccgcaggtc ttcatcaata aaactgtttg
    35101 gttctagttt aagcgtcgtt aaatcacaca gtttgcgcag cggcgcggga agatgaatat
    35161 taataaaatt ccgcgcggtg tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg
    35221 gcgtggaagt tgtcgatatt gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg
    35281 acaataacca cgattaacac ccaatgcagc aggcagtttt tatctttgga cagcgaattc
    35341 agaagtattt ttagcggact gcaactttta catttcagcg gaagctgact cgcaaattcc
    35401 ccttgaaaat actccggcag cgttagccac cggagaggaa attatttcag cgatccctta
    35461 aggaactgtt gcagacgagg gctttgcggg ttgccaaata actgctccgg cgcgccctct
    35521 tcttctattt tcccctgatg gaggaaaatg acgtgagtag aaacatggcg agcaaagccc
    35581 atttcgtgag tcactaccac catggttttc ccctcttctg ccagttgctg cataatacgt
    35641 aacacttcgc ctaccagttc aggatcgagc gccgaggtag gttcatcaaa cagtaaaact
    35701 tctggttcca tcgccagcgc ccgcgcgata gaaacacgct gttgctgacc gccggaaaga
    35761 tgcaccggat atttcccctg cgcacgttcg tctatcccga ctttcgccag atacttcacc
    35821 gcccgctcgc gcgcttcctg cttgctcagg cccaacacct gaatcggcgc ttccatgacg
    35881 ttttccaaca ccgtcatatg gctccagaga ttgaagtgct ggaataccat cgtcaggcgt
    35941 gtgcgcagta agcgcagttg atttttatcg gcgactttga gttgaccgtc tttgtcgcgc
    36001 accagattga tcgtctggcc attgaccacg atcgatcctt cactcggttt ttcgaggaag
    36061 ttaatgcagc gcagaaaggt acttttcccc gatcccgacg atccgatgat gcttattaca
    36121 tctccggcat tcgcttgcag tgagacccct ttcagcactt cgtgttcgcc atagcgtttg
    36181 tgcaaatcga taacgtttaa tttattctcg gacatcgtat tctcagtgcg ttgaagaagg
    36241 tttcacatgc tgcaaccagc gtttttccgc tctgcgaaag aggctgatca ggacataaga
    36301 gatgattaaa tagagcaccg cggcaatgcc gaaggcggta aaaggttgat acgtggcggc
    36361 gttaatatcg cgggctattt tcagcagatc cggcacagtg gccgtaaacg ccagcgcagt
    36421 agagtgcagc atcaggatca cttcgttgct gtatgccggt aacgcaatac gcagcgcaga
    36481 aggcaaaata atgcagcgat acattttaaa ggtcgagaag ccataggctc tggcggcttc
    36541 aatttcccca tgcggaaccg aacggattgc cccagcaaaa atctcggtag tgtaagcgca
    36601 ggtgttaagc gtcagcgcca gcacggtaca gttcaggcca ctgcggaaga aagcgttaag
    36661 gaattcggtt cccttaacaa tctcaagcgt gtacatgccg gaatagaaca ccagcaactg
    36721 aacatacagc ggcgtaccgc gaaaaatata ggtaaataac cagattggaa actggatgta
    36781 tttattactg gagacacgac caatcgccag aaacagcgcc aggactccgc ctatcactac
    36841 cgacaaaata agcagccaca gagtgatcgc cacaccagta aagcgataac cgtcggtcca
    36901 cagcagcggt ttccagtatt catgtaagat ttcgatcaca ggtcagccct cttcacaccc
    36961 acggagtagc ggcgctcaag gaacagcagc acaccattgg aaacggtggt gaaaaccagg
    37021 taaatcacgc cacagacgat ggcgaaatag aacggttccc aggtactttt gcctgccagc
    37081 tgcgtggctt tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt agatttgagg
    37141 atcacctgcc agttgttgcc aatgcctggc agggcgtaac gcatcatcgc cggaaacatg
    37201 atccgccgaa acacttgccc gcgagtaaaa ccgaacgccg tcgccgcctc tatatgccct
    37261 ttcggcactg ccataaacgc gccacggaac gtttcggtaa aataagcacc gtagataaaa
    37321 ccgagagtga taataccagc gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc
    37381 tccgtcacca tgtttagcgc aatctgcaaa ccatagaaaa tcagcagcat caacaccaag
    37441 tccggcacgc ctcgaatcag cgtggtgtac ccttcgaaaa tcagccccga aagccgattt
    37501 tgcgagagct taccgccagc gccaattaaa ccgatgatta cagcgagcac tacagagctg
    37561 atagccagct ccagcgtgac gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac
    37621 atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc cggttacccg
    37681 aagggagcgg gctttctttc accgatggcg attaaccacc ataaacatca aaatcgaagt
    37741 actttttcgc taatttctcg taagtaccgt cagcgcgcat ttcggcaaag gctttgttca
    37801 gtgcctcgcg cagttcgtta tcttctttac gcaggcccat gccggtccct acgccaaaca
    37861 gtttttcatc tttaacagac ggaccaccga atttgtaatc tttaccgacg ggttgtttga
    37921 ggaaaccttc gctggcagcg acctcatcct ggaacgcggc atcaatacgt ccggcagtca
    37981 ggtcagaata aatgttgtcc tgcccctgat acgagacgat ttcaatgcct tttggtgccc
    38041 agtgttcatt accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc cgtttgcctt
    38101 tcagcgactc gactgtcggc tgaatgtcag aatttttcgc caccaccaga cgagaatcgg
    38161 cagcgtacag tttgtcggtg aaggcaattt cttgctgacg tttttccgta atggaaagcg
    38221 atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca tccagcgggt
    38281 tttcgacaaa cgtacattgc gtattgatgc gcttgcacaa ctccttcgcc agctcgatat
    38341 cgaaaccaac cagttcgcct tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg
    38401 taccgatgcg gatgttttgc ggaattgcag caagtgccgc agttgcgctg gagaaggcca
    38461 gaaccagaga gagcgatagc accagttttt tcataatgtc ctcaactgac agtcttttta
    38521 tagggaatat ttacaggttt gttacactta tcgtgccata aaattgacca tcaaggcaaa
    38581 atatcccgcc ccgttagacg tattattttt cattaaaaat gcttaagtat gcatttcaat
    38641 gcaccacgat agtgcaacaa acctggcgtg caccatgatg atcattccat caggtacagc
    38701 ttcccagcga cgtatcagtc accgtagaca ttaaagtcga aatacttttt cgccatcttg
    38761 tcgtaggtgc cgtcctgacg cagctcgcca agcgccttat tgaaggcagc cgtcagttca
    38821 gcataatctt tacgtagccc tacaccggtg ccatcaccga agtatttttt gtcttttact
    38881 gatgagccag caaaagcgaa atctttacca gcaggttgct tgaggaatcc ttcgctggca
    38941 gcaacttcat cttgtaacgc agcatccaga cgtcctgcag ccagatcgga atagaccaaa
    39001 tcctggttgg cataggccac cacatccacg cctttactac gccaggtctc gttagcgtaa
    39061 gcttcctggg ttgatccctg cagcacacca acatgtttac ctttcagtga atccagcgtt
    39121 ggctgaatcg gtgaaccttt ggcagcaatc aaacgagaat ctgcggcgta cagcttgtcg
    39181 gagaaggcaa tctcctgctg acgtttatcg gtaatggaaa gcgacgaaat aatagcgtcg
    39241 atttttttcg ctttcagtga ggggatcagc gcgtcaaagt cactggcaac ccaggtacat
    39301 ttcacctgca tccgtttgca catctcgtta ccgagatcga tatcaaagcc aacaaaatca
    39361 cctttagcat ctttcgacga gaacagcgca taggtggtat cggttccgat acgtaccgtc
    39421 tccggtagcg ccgcatagct ggaagccgct gcggagagac cgactaataa agacagagcg
    39481 agaatcgact tcttcataca taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg
    39541 tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc atcttgacga caacagtaac
    39601 attcaacgtt aaatatgtta ataagacatt gcattattgt cctgaagttg aagatagcag
    39661 gtatagcggt tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg
    39721 atcgcggttg caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc cctgccagcg
    39781 ggcaaagaga tcttcaggaa gggttattgc aaactggtca agaacacgat taaccgtctg
    39841 atttatcaca tcatcaaggg attgcgggcg atgataaaac gccggaacgg gaggcataat
    39901 caccgcaccg atttctgccg cctgagtcat taaacgcaga tggcctaagt gcaatggtgt
    39961 ttcacgcacg cagagcacca acgggcgacg ctctttcagc accacatctg ccgcacgggt
    40021 cagtaagcca tcagtatagc tatggacaat gccggacagg gttttgattg aacagggtaa
    40081 aatcaccatc cccagcgtct ggaaagaacc ggaagagatg ctggcggaaa tatcgcgcgc
    40141 atcgagcgtg acatcagcta atgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga
    40201 taaggtctgg cgcgccgcct ggctcatcac cagatgcgtt tcgatatctg tgacatcgcg
    40261 cagaacctgt aataagcgca cgccataaat cgcgccgctg gcaccgctga tgcctacaat
    40321 gagtcgtttc ataaatagtt gccctgtcag acttgcaggc agactttgca gtatttcggc
    40381 gggagttgca agtcagggtg ccagaccggc accctcagcg aaggcatcat ccttcgttat
    40441 gcatttcgag attttccact tcgttctgac gttgcactgc tttggcgtca tcattacgta
    40501 acgtatcgag gaaatcgagg tagccctgat caacatcttt ggtgacgtag acgccgttga
    40561 acaccgagca ttcaaactgc tggatatccg gattttcagc gcgaacggcg tcgatcagat
    40621 cgttcagatc ctggaaaatc aacccgtcag caccgatgat ctggcgaatt tcatcaactt
    40681 cgcgaccgtg agcgatcagt tccgtggcgc tcggcatatc aataccataa acgttcggga
    40741 agcgaatttc cggtgccgca gaagcgaggt acactttctt cgctccggct tcgcgtgcca
    40801 tctcgataat ctgctcagaa gtggtgccac ggacgatgga gtcgtcgacc agcaggacgt
    40861 ttttatcgcg gaactcggcg cggttggcat tcagtttacg gcgcacggac ttacgacgca
    40921 gctgctggcc cggcatgata aaggtgcggc caacatagcg gtttttaacg aagccctggc
    40981 ggtacggttt gcccagaata cgagcaattt ccagcgcgat atcacacgag gtttctggga
    41041 tcgggatcac cacgtcgata tccagatctt cccattcgcg ggcaattttc tcgcccagtt
    41101 tcgtgcccat attcacacgc gcgctgtaaa cggaaatttt gtcgataaac gagtccgggc
    41161 gggcaaagta tacatactca aacaggcacg gattgctgac cggattgtca gcacattgac
    41221 gggtaaacaa ctgcccttct tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca
    41281 ggaaatcaaa gcccagcgta tcgagcgcta cgctttcgga agcgaccata tattctgtac
    41341 ggttctcgtc aatatcacgt tttcccagta ccagcggacg aatcccgttt ggatcgcgga
    41401 aagcaaccat accgtggccg ataatcatcg ccacacaggc atacgcgccg cggattaagc
    41461 ggtttgtggc agcaatggca gcgaaaatat tgtcggcttc cagcgggtag tggcggaagt
    41521 tgtccagctc gctggcgaag atattaagca gaatttccga gtcggaagtg gtgttgatgt
    41581 ggcggcgttt ttcttcaaac agttttttac gcaactcgtg agcgttggtc agattgccgt
    41641 tgtgggcaag cgtaatgcca tacggggagt taacgtaaaa cggctgcgct tcagaggcgc
    41701 tggagctgcc agccgtaggg taacgcacat gaccaatgcc catattgccc tgcaaacgct
    41761 gcatatggcg agcttcaaat acatcgctca ccagcccgtt cgctttacgc aaacggaagc
    41821 agttattggc atctatggtg atgatgccgg cggcatcctg accgcgatgc tgaagcaccg
    41881 ttaaggcatc ataaatcgac tggttaaccg gcataacacc ggcgataccg acaataccgc
    41941 acatacgtct ttttcctcgt taagccacat ctcagagcac ttacgctctg ggcaagaaac
    42001 ttgacgagct ttgcagataa tcaaaaaacc atctgatgat aaaactgaat tgcgggatca
    42061 gctgtgattt gctccagtct tcacttttcg acaccccggt aaaggagtcg agaaagaaga
    42121 gaatggcggc aacaatcaac acaccgcgca acgcaccgaa acagacgccc agcacccgat
    42181 cggtgcctga caaccccgtt ttctccacca actggcctat cacgaagttc acgatagcac
    42241 caacgatcag ggtagcgata aacagtaccg cgatggcaat cccatttcga accagttcgt
    42301 cttcaaagcc cgtaaaccag actgacaggt aagtgtagta atgactggca acaaagaaag
    42361 cacaacccca tgtcaccagc gataacgctt cacgaacaaa gccgtggatc aggctaacca
    42421 gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat ccagaccata tgtgtcccac
    42481 gattttacgc cctgtcatcc tgttcggggc gcattctaac agaaaaagaa aacgtttgcg
    42541 tagggatttc cttcccgcgc atcaataaaa atggcgctga aaaaatattc aacgccatcg
    42601 actttttatg cctttgcggc atcgtgcaat gcgtgtcgga tgcggcgtaa acgccttatc
    42661 cgacctacgg ttctaacact gcccaagcct gataagacgc gccagcgtcg catcaggcaa
    42721 gactgtatta attcggcgta tagcccatta ccacgccact taagccagaa agttgcttca
    42781 actcacccag cgaacctttc agcttatctt tcgaggcatc cggcccaacc agaatacggg
    42841 taattttacc ctgcactggc gtggatggcg acgtataaac ccgataaccg gcaccgcgca
    42901 gcttaccgac aatctcattc actttatcgg catttttcag cgcacccagt tgcacaacat
    42961 aggctttacc cgttggcgct gctttttctt ccacgaccgg cttcggttct ggcttcggcg
    43021 caggtggtac ttcaaccttt ggtttaggcg gctccaccgg tttcggcttc ggtggggcga
    43081 ccggtgcagg ttccggttca aactcggtgt tattggctgc aatagtggcc ggatcgagcg
    43141 acggtgctgc ggcatcacct gcccgcacct cttccgctgc gccttccggc ggctgcgtcg
    43201 gcaacgcctg ggtggcggct ggcatcatat caggctcatc acgatcgccc gctttcggca
    43261 ccagcgggat agccgcgaac tcatcctgat aatgtttttt ctgcccgtcc agcagccctg
    43321 gaagtacaat cacccccagc gccaccagca cgatcgtgcc cactaaccga ttctgaaact
    43381 tacttgccac cgcttctcct cgcgtcaatc acttccatga catgtgcgac cgtgtggaaa
    43441 gaaccacaca ccagcacggt gtcttccgct ttagcgtccg ccattgcggc atcccatgcc
    43501 tgcgcaacgc tatcaaatga tttgccgtta cccaaatgct caagcagttg ttctgccgtg
    43561 gcaccgcgcg gcccttccag tggcgcacaa taccagtcat caaccacgct tttcaaccag
    43621 gccagagttc cggcaatatc tttatcatgt agcataccga taaccgccag cacgcgcccg
    43681 tttttcggta gcgctttcat acgcccggtg agatattccg ccgcatgtgg attatgcgcg
    43741 acatcaaaaa taacgcgtgg cgactcgctc acaatctgga aacgtcccgg caaaattgcg
    43801 ctggcaatcc cgtcgcgaat ggcattttca ctgacttcca gcccgctggc acgcagtgcc
    43861 gccagcgccg ttgcggcatt cggttgcggg acaagcggca acggcagatt ttccagcgtg
    43921 ccgtgagcat cgctaaacgc ccagtcatga tcggtgacgg aatagttcca ctcaacgccc
    43981 cgacgttgta acagtgcacc tttttcctgc gccacatcag caatggtaga aggcatttcc
    44041 ggctcaccga caattgccgg tttttcgctg cggaagatgc ctgctttctc gagaccaata
    44101 ctttcgcgat ctggacccag ccagtcggta tgatccagcg caatactggt tactaccgcg
    44161 acatcggcat cgacaatatt ggttgcgtcc agacgaccgc ccagccctac ttccagaatc
    44221 accacgtcaa gttgtgcctg cttgaacagc cacaacgccg acagcgtacc gtactcgaaa
    44281 taggtcaggg aaatatcacc gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc
    44341 gattccggca attcctggcc ctgcacacgt acgcgctcgg tataacgcac cagatgaggc
    44401 gaactgtaga cgcccacttt gtaccctgcc gccatcagaa tcgactccag cgtacggcag
    44461 gtggtgcctt tgccattcgt acccgcaacg gtaaacacaa atggcgctgg tttcaggacg
    44521 ccaagacgcg ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt tttactgtgc
    44581 aggttttcca gataagaaag ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg
    44641 ataatcatgg tatccgctga ttcgttacgg tgagaatagc aaaagggcag agccagtggc
    44701 cctgccctta tcagttatca ggcctcaggt tcctgatccg gtaccggggg taccactacg
    44761 ccttcacgcg gcgcttcagg attcggcgct ggcagattca tcaacttcgc cagaatgctc
    44821 gccagtttca ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc
    44881 aggaattcac tgcgctggaa tctaggcggc agtttttcgc gaacggtctg ttcgataaca
    44941 cgcggaccgg caaagccgat taacgcttta ggttcagcga tgttgagatc gcccagcatg
    45001 gcgaaacttg cagaaacacc gcccatcgtc gggtcggtca gcacggagat gtacggcaag
    45061 ccgcgctcct gcatttttgc cagtgccgca gaggttttcg ccatctgcat cagcgacatc
    45121 agtgcttcct gcatacgtgc gccaccagag gcggagaagc agatcagcgg gcagttatct
    45181 tcctgcgcct gctcaacggc acgcacgaaa cgtgcaccca caacagaccc cattgaaccg
    45241 cccataaagg cgaactcgaa tgccgcagcg acaaccggca ttccatacag agtgcctttc
    45301 atcaccacca gcgcatcttt ttcgccggtt tctttctgcg cagatgccag acggtcttta
    45361 tacttcttgg agtcacgaaa cttcagctca tctttcggct caagctcgct acccagctcc
    45421 acaaggcttc cttcatctaa caggctatgc aggcgattac gcgctgtcat acgcatgtga
    45481 tggtcacact tcggacagac ctcaagatta cgttccagct cagcgcggta taaaacctga
    45541 ccgcagctat cacacttagt ccacacccct tcaggaatgc tcgccttgcg ggtgggagta
    45601 atgttgcttt taattcgttc aatccagctc attagggacc tttctgtctg aacctggttc
    45661 gatgccagtt ttatctttgg ggacgcataa tgccattttt gcccccaaca gaccatgaat
    45721 gttgcacatt aaaacataac agcccgaaac tttggataaa aaagtggtcg aaccgcggag
    45781 ttacttttta ttttgcggcg cgtgccgcag cgcgtttgtg acggattatt tcgatgacgc
    45841 ccggcaaaat agaaaccaca ataatcccga cgatcagcag cttaaggtta tcctgaacca
    45901 tcgggattgt accgaagaaa tagcctgcgt aggtaaaaag cagtacccac aacagtgcgc
    45961 cgatcacgtt ataagcagcg aaatgacggt acgacatgtg gcccattccc gcaacaaacg
    46021 gggcgaacgt tctgacgatc ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt
    46081 tctcataaaa ctgatgggtt ttgtcgagat aactgcgacg gaaaattttc gaattgggat
    46141 tactgaataa cttttcaccg aacaaccgcc cgatagtgta attgaccgcg tcacccacaa
    46201 tcgcggcgat cagcatcagc actaccatca tatggacgtt gagatcgttg gtttccagtg
    46261 atgccaacgc tccggcaaca aacagcaatg aatcacccgg taaaaacggc attaccacca
    46321 gaccggtttc acagaacaaa attaagaaca aaatggcata aacccagacg ccgtactccg
    46381 cgactagctc tgccagatgc acgtcaatgt gcaggataaa atcgatgagg aaataaatca
    46441 ggtccatatt tgcctatgcc ttgtactcgt catctttcag gctgtaacta cgttggctac
    46501 agcctgaaat gttccgagca ttattctcgt tagtccgcca gaaatagcgg gcccattggc
    46561 ggttttggaa gatcataccg gtcagggtaa tccaccgcga ccagatacag cccttccgct
    46621 tttgccgttg ctgccgccag cgttctgtcc tttgccgcca gcaactctgc tatccagctc
    46681 tccggctggt tgtgggcacc gacttccatc aggctgccga caatattcct gaccatatga
    46741 tgtacaaagg cattcgcttt gatatcaacc accacataag gaccgtgacg cgtgacatta
    46801 atatgcataa cgttgcgcca cggggttcgg gactggcact gcaccgcacg gaacgaggtg
    46861 aaatcattct cgcccagcaa gcattgcgca gcccgatgca tccgttcagc gtccagcggt
    46921 tcgtaaaaat gggttacccc tttactcagt accgccgggc gcagccgatg attgtagatg
    46981 atgtagcgat aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc aggtacagct
    47041 ttaacccaac gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa ggtccacgcc
    47101 gcgtctttgc gctgcgcggt tgtttcgaaa tgcaccactt gcccggtacc gtgtacccct
    47161 gcgtcagtac gcccggcgca gaagacggtg atgggttcgt tcgccacctg ggagagcgcc
    47221 ttttccagct tctcctgcac actgcggact tcattctgcc gttgccagcc gtaatactta
    47281 ctgccgtcgt actcaatgcc cagcgcaatt ttataaactg gcagttgttg ctggtcggac
    47341 attagtacag atactcctgc accagtttct cggcgatttt tactgccatc agcgcgccgc
    47401 caaagcgaac gttatcggcc accgaccaga actggacttg ctccggcata ccgtagtcat
    47461 tacgcacgca gccaacagaa agatgcggcg tacccgaagc atcccctacc tgagtcggga
    47521 attcgttctc ttcagagagc acaatatctt cgccctgagc aaacgcatca cgcgcttctt
    47581 ctgccgccag cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg tagaataccg
    47641 gtgcctggac gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt ttgcgtactt
    47701 cgtcaacgat acgacgttct tcacgcacgc taccttcgct atccggcaac aacggcagca
    47761 tattgaacgc cagttgacga ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca
    47821 atttcgcact ctgccccgct aacgcatcga ccgctttttt gccctgggcg gaggctgaaa
    47881 tcaggctggt aacactgata cgcgacaaac cgccctgatc gattaacggt ttcagtgccg
    47941 ccagcagctg gctggtcaga ctgtctggta cggcgatgac attccggttg cggtaatcgg
    48001 tcagtacaaa cgggtttact tccggcacca ccagcggtac gtcgggttcg agagcaaaca
    48061 gtccgctgct gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc caggcagcgg
    48121 tagcttcttt gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg aattcagcgg
    48181 catcctgcac ggtgattgtt ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac
    48241 gtgccagtgc ataaatttcc ccaaccggga actgacgttc agccagcgtt tcaagcaggg
    48301 cttcgcccat agcgccagtt gcgcccagga cggcaatgtt ccagccttca gacatggtgg
    48361 tttactccag aaatagcaaa gctccctgcc aatgttacag cagagagcat gaagaagaga
    48421 ttaacgtgcc ggatgatgaa cggcgttaaa acccagttta cacagcaatg atgccgcact
    48481 ggcgtcatca caaattacat acagagatga ccattcacgg cgctcaagat agtttttgcg
    48541 cagtttatcg aactcacccg gtatcccggc gactttacgc agcggtgcgt catcgcggcg
    48601 cacatcatac accaaatgca ccagcctttt cagcgtcggt tgatcgagcg gaccatgcag
    48661 cgtaatgcgg ccaaactcag gcgcgggcag caacgtatcc agcgcaacgt gttgttcatg
    48721 cccaataaac ttgctatagg cttcaaacac ttgcgtcgtg ccacgcgctt taccctccag
    48781 ggtatagcct gcgatatgcg gcgtgccgat atccactttt gtcagcaatt ccacattaag
    48841 ttccggctcg ccttcccaga catccagcac cacgcttaac ttctggcctt cattcaggca
    48901 ggtcagcagc gcggtattat cgacgactgc gccacggcag gcgttaatca gaatcgctcc
    48961 gggcttcagg ctacggatca gtttttcatc cgccagatgt agcgttttgt acggaccatc
    49021 tttaaagagt ggcgtatgga aagtcagaat atccgcgcgc tggactaact catccagcga
    49081 gcggaaatca ccctcatccc cacggtcggc gcgaggcgga tcgcaaagta aggttttaat
    49141 ccctaacgct tccagtcgcg cctgcaatcg acgtccaacg ttaccaacgc ccacgatccc
    49201 caccgtacgg tcgtgcagtg aaaatccatc gcgttcggca agcatcagca gggaggagaa
    49261 aacatattcc accaccgcaa tcgcattaca gccaggtgca gcggaaaaac caattcccgc
    49321 ctgcttcaac catgcttcat cgacatggtc ggtccccgca gtggcagttc caacaaattt
    49381 aatgggtttt cctgccagca aagattcatt cactttcgtg accgaacgca ccatcagcgc
    49441 atccgcgtct gccagttgag cgacggggat tggacgcccg ggaaccgcga tcacctcacc
    49501 caaacggcta aataagtcgc gggcataagg catattttca tcaacaagga ttttcacgtt
    49561 tgtgttacct gtatgagacg agagttaacc ggacaagtat gccataatct cgcggccagg
    49621 catacttgcg aagatttcag gtataaggat acgtaatgat acaacctatt tccggccctc
    49681 ctcctgggca gccaccaggt cagggagata acctgccgtc tggcgcgggc aatcagcctt
    49741 tatccagtca gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc
    49801 aacaacaaag agcagaactg tgggcgggta tcaggcacga tattggtctg tcgggagatt
    49861 caccgctgct ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg
    49921 ccgcgcaaaa aagccattct gcccgccagc ttttagcgca attaggggag tatttacgtc
    49981 tggggaatca tcgtcaggcg gtcacggatt atatccgtca taactttggt cagacgccgc
    50041 tgaatcagct ctcaccggag caattaaaaa ccattctcac cctgttgcag gaagggaaga
    50101 tggttattcc gcaaccacag cagcgcgagg cgaccgaccg tcctttatta ccggcggagc
    50161 acaatgcgct aaaacagctg gtgaccaaac ttacggcggc aacgggggaa cccagcaaac
    50221 agatctggca atcgatgctg gaactttccg gggtgaaaga tggcgagtta attccagcga
    50281 aactgtttaa ccatctggtg acctggctac aggcgcgtca gacgctaagc cagcaaaata
    50341 cgccgacgct ggaatcacta cagatggcgc taaaacaacc tttagatgcc agtgaactgg
    50401 cggcgttatc ggcatatatc cagcaaaaat atggcctttc tgcgcaatca tcgctttctt
    50461 ctgcccaggc cgaggatatt cttaatcagc tttatcaacg gcgggttaaa gggattgatc
    50521 cgcgtgatat gcaaccgctg cttaatcctt ttccaccgat gatggacacg ttgcaaaata
    50581 tggcaacgcg tcccgcgctg tggatactgt tagtcgcgat tatcctgatg ctggtctggc
    50641 tggttcgtta accccgacga aatgacagta tcgtgacaat aatacccagc accgcagaga
    50701 tcgccccggc aagaaatacc gaagagtaac caaacgtggt cgccagcatt cccgccagcg
    50761 gcccggagac gccgagggcg atatcctgaa acgcggcgta accgcccagt gcggtgccgc
    50821 gaacttgtga ggggacgcgt ttaaccacct ccacgcccag cgcaggaaag ataagcgaac
    50881 atccggctcc ggttaacgcc gcgcccgcta atgcgaccca tgcacctggg gcttgccaga
    50941 gcagcaacaa gcccaccgtt tctacaagca gagagacaat cgccactttc acgccgccaa
    51001 aacggtccgg catccagcca aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg
    51061 taagagtaaa gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc
    51121 cgataaccgc aaaaccaacg ccttgtagtg ccagccctaa ccctggtttc cagataagcc
    51181 cgacaacgct ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc
    51241 cgttacaggc ccacgccagt aagggtaata ccattgtggt gatcgccagt gcggcaaaac
    51301 cgtaatggct atgaatcaac aggccaagcg gcgcaccaac agcgagggca ccgtaaatcg
    51361 ccattccgtt ccatgacatc actttgccag agtgttttgg ccctacgatg cctaaccccc
    51421 aggtcagagc gcctgtcagt aactggcttt caccaaaccc aagaatcaaa cgcccgatga
    51481 ccaacagggc aaatttgaac ggtgcggaga caggcaaaat cgccgccagc agcaacgcgc
    51541 cgccagccag accacaagct aacatcccct gaagcgccga acgttttgcg ccatattgat
    51601 cggccagtcg cccggcgtaa ccacgcgtca gcaccgtagc cagaaactga atcccgacgg
    51661 caatgccgac catggtattg ccatagccca gatcatgatg aacaaacagc gggataaccg
    51721 gcaacggcaa ccctacggtc atgtaggtga gaaaaaccgc aaaagagatg cggaagagcg
    51781 aaaaactggc agaagatcgt gtttcggttt ggcttacagc agtcatgcat tactccagaa
    51841 tgcagcgcaa ggcgaggagt atccccgtct catctctctg gtttcagggt tacggtgcgt
    51901 tggcaggatt taacgcgtac gtcttttcag aaggaaatcg acaaagcggg aagtttgcct
    51961 agaactggtg gcgattgtca atgatgtgaa aaagggaacc atcaggttcc cttttgcgtt
    52021 agtgccggag gccgcattgg cgcgtaacgt cggatgcgac gctggcgcgt cttatccgac
    52081 ctactccggc ctactgcgaa ttaatctttt cagcttgcgc attaccagcg tggcgttggt
    52141 gccgccgaag ccgaagctgt tagacataac agtggtcagt tcgcgatcgg tcgtttcggt
    52201 cacgatgttc agacccgcag cctgctcgtc cagctcttca atgttgatgc tcggggcgat
    52261 aaagccgtgt tccagcatca gcagagagta gatagcttcc tgcacacccg cagcgcccag
    52321 tgagtgaccg gtcatggctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc
    52381 acggatagct gccagctctt tcacgtcgcc aaccggagtc gaagtaccgt gagagttcag
    52441 gtaatcgatt ggggtatcaa cgccatgcat cgccatctgc atacaacgta ctgcgccttc
    52501 gccagacgga gcaaccatgt ctgcaccatc agaggttgcg ccgtagccaa cgatttcagc
    52561 atagatgtaa gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta ccataccgcc
    52621 gccgccagcg ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg
    52681 ggtgtcgttg tatttagtag atagcgcacc catcgcgtcg aattcgcagg ccatttccca
    52741 gcacagctct tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc
    52801 tactgcgtta ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac
    52861 gccatgaatt ttaaacggcg tggcgagaca ggcagaaacg ccggatgcca tcgctttagt
    52921 gaccacatac gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa
    52981 cacctggaaa cgcggggaac cgccgccgga acctgcaatc aggccaacgc gcgggttatt
    53041 ctggtaagct tccggagaga ggcccgcatc agcgattgcc tgctccatag aaaggaatgc
    53101 ataaatggat gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc
    53161 cagttttatg ttgccccaga cgtggctacg catgccggaa tccttcagct cctgagagaa
    53221 agtgatccct gaacgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat
    53281 gctggaaaca atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc
    53341 gcacatagag taagtttcga atgcacaata gcgtacactt gtacgccgaa caagtccgat
    53401 cagccattta atagagatat ttgcgcagcc ttacacacat cgctaagatc gagccaccgc
    53461 ctgtaagacg agtaacttac gtgaaacact actccataca acctgccaac ctcgaattta
    53521 atgctgaggg tacacctgtt tcccgagatt ttgacgatgt ctatttttcc aacgataacg
    53581 ggctggaaga gacgcgttat gtttttctgg gaggtaacca attagaggta cgctttcctg
    53641 agcatccaca tcctctgttt gtgatagcag agagcggctt cggcaccgga ttaaacttcc
    53701 tgacgctatg gaaggcattt gatcagtttc gcgaagcgca tccgcaagcg caattacaac
    53761 gcttacattt cattagtttt gagaaatttc ccctcacccg tgcggattta gccttagcgc
    53821 atcaacactg gccggaactg gctccgtggg cagaacaact tcaggcgcag tggccaatgc
    53881 ccttgcccgg ttgccatcgt ttattgctcg atgaaagccg cgtgacgctg gatttatggt
    53941 ttggcgatat taacgaactg accagccaac tggacgattc gctaaatcaa aaagtagatg
    54001 cctggtttct ggacggcttt gcgccagcta aaaacccgga tatgtggacg caaaatctgt
    54061 ttaacgccat ggcaaggttg gcgcgtccag gcggcacgct ggcgacattt acgtctgccg
    54121 gttttgtccg ccgcggtttg caggaagccg gattcacgat gcaaaaacgt aagggctttg
    54181 ggcgcaaacg ggaaatgctt tgtggggtga tggaacagac attaccactc ccctgctcca
    54241 cgccgtggtt taatcgtacg ggcagcagca aacgggaagt agcgattatc ggcggtggta
    54301 ttgccagcgc gttgttgtcg ctggcgttat tacggcgcgg ctggcaggta gcgctttatt
    54361 gcgcggatga agcccccgcg ctgggtgctt ccggcaatcg ccagggggcg ctgtatccgt
    54421 tattaagcaa acacgatgag gcgctaaacc gctttttctc taatgcgttt acttttgctc
    54481 gtcggtttta cgaccaatta cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt
    54541 taggctggga tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg gatttacccg
    54601 cagaactggc tgtagccgtt gaggcaaatg cggttgaaca aattacgggc gttgcgacaa
    54661 attgcagcgg cattacttat ccgcaaggtg gttggctgtg cccagcagaa ctgacccgta
    54721 atgtgctgga actggcgcaa cagcagggtt tgcagattta ttatcaatat cagttacaga
    54781 atttatcccg taaggatgac tgttggttgt tgaattttgc aggagatcag caagcaacac
    54841 acagcgtagt ggtactggcg aacgggcatc aaatcagccg tttcagccaa acgtcgactc
    54901 tcccggtgta ttcggttgcc gggcaggtca gccatattcc gacaacgccg gaactggcag
    54961 agctgaagca ggtgctgtgc tatgacggtt atctcacgcc acaaaatccg gcgaatcaac
    55021 atcattgtat tggtgccagt tatcatcgcg gcagcgaaga tacggcgtac agtgaggacg
    55081 atcagcagca gaatcgccag cggttgattg attgtttccc gcaggcacag tgggcaaaag
    55141 aggttgatgt cagtgataaa gaggcgcgct gcggtgtgcg ttgtgccacc cgcgatcatc
    55201 tgccaatggt aggcaatgtt cccgattatg aggcaacact cgtggaatat gcgtcgttgg
    55261 cggagcaaaa agatgaggcg gtaagcgcgc cggtttttga cgatctcttt atgtttgcgg
    55321 ctttaggttc tcgcggtttg tgttctgccc cgctgtgtgc cgagattctg gcggcgcaga
    55381 tgagcgacga accgattccg atggatgcca gtacgctggc ggcgttaaac ccgaatcggt
    55441 tatgggtgcg gaaattgttg aagggtaaag cggttaaggc ggggtaatct gctctcgcag
    55501 tgtttgccgg atgcggcgtg aacgccttat ccaacctacg tgtgacgtgt aggcctgata
    55561 agacgcgata agcgtcgcat caggcatggt gctccagacg ccgcaactta cagctgtgac
    55621 gcctgctgaa acaggttttc ccacatatcg gtcaccagcg cctggtcacg cggcgacaat
    55681 tcaccggcac caatggcttt ttccagactc tggctaacgg tcgtatgcac cgcctgagcg
    55741 gagtggtcat caccactttc cagttctgcg atggctaatg tcaggtggcc acgcaaatac
    55801 ccactggcaa acaactcatc atcactggca tggtcaacca tgccgtcgat taatgccaga
    55861 atgcgtgatt caaactccgc gatcatcttc tttcctcatt gtaaaacgtg gcgctggctt
    55921 cagttgagcg cttccggcca cgggaactgt tccgccgtaa gttccggcgt gtgataataa
    55981 ttttgtagtg ctttaatgaa gcgtgccggg cgttccggaa tgccatttgt cagataatcc
    56041 atcacctgcg catgtacccg ccgttgaaac accacacgat ccggttcgaa atccccttcc
    56101 agattgtcgc aactgacgtt aaacggatat ccagccgcca cgcagaataa ccagtcgaac
    56161 gcctgcggct tcacttcaac atcttcaaac tggctttgcg tctgggcatc gcgtccgtcc
    56221 gggcaatacc agtagccgaa atcaaccagc tcacggcgcg cttttccggc aatacaccag
    56281 tgcgaaatct catgaattgc gctggcataa aagccatgag caaagacgat tcggttatac
    56341 ggtacttccg catcagcagg aagatagatc ggttcgtcgt cgcctttaat cagacgggta
    56401 ttaaattcat cggcaaagca gctattaaaa atttcaatca actgctcgta gtggtgtgta
    56461 ctgttcatta gttcatcccc aaccagtgga ggatctcctg tccgtggcta tcgtaaagta
    56521 atttggcact catcaccgcc gagacgataa caatcatcgg gcggatcagc ttttgtcctt
    56581 tgctcaacac caaccgtgaa cccatacgcg cgccgaggaa ctgccccacc agcatgacaa
    56641 agcccgtcgc ccaaatcact ttgccgccga gaataaacag cagcagaccg ccaatgtttg
    56701 acgttgcgtt gagtaatttg gcgtgagccg tggctttggc gaggttgaat ccgcacagca
    56761 taacgaaggc cagcgcgtaa aacgatccgg cagccgggcc aaagaatccg tcataaaaac
    56821 cgacgcagcc accggcaatt aacgcgaagg gtaaaccgta catccggcgc tggcgatctt
    56881 cttcacccag ctttggcatc agcaagaaat agaggccgat acaaatcacc agaatgggca
    56941 aaatctgccg taagacatca gcctgaacgt actgtaccaa cagtgcgccg ctcattgagc
    57001 cgacaaaggt catggcaata ttgagtttct gatcgcttaa actaaccact ttgcggcgaa
    57061 taaagtagat agtggcggaa atagagccgc cgcaggcttg cagtttattg gttgccagcg
    57121 cgttagcggg agacattccc gcagccatca atgccggaat ggtgagtaac ccaccgccac
    57181 cggcaatcga gtcgataaat cccgccagca tggcgacaaa aaagagaact cccagcaaca
    57241 gcggagaaac cataaacagg ctattaaacg tttccattag atcacgtgct catccagtag
    57301 cgcctggcag gaaggcggca acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg
    57361 ttttggaggt tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg atggcggtaa
    57421 aggttgatct tcacactcca gactatcggc agggcaacgt aatcgtacat gcatatgcgc
    57481 gcgatgctgg aaccagggtc gcactttgcg caaccagtcg cgatcggtgc ccgcatcaag
    57541 gcaaagttgt tgtttaatcg ccggattaac aaaaatgcgc gtgacgtctt tgtcctgggc
    57601 ggcgagtttg atcaagctga aaatttctgg cttccacagc gtggagacaa cgtgtttacc
    57661 gtcgcgggaa actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg tccagcgagt
    57721 tttcggcagt tgcagaaaga tatcgacatc cagtccggtc tggtggctgg catgaccgcc
    57781 gttgaaacgc ccaccagcgg gcatccccat atcgccaatc agcaccgtac ccatgcccag
    57841 attgctcacc tggctactca gacgctggat aaacatcacc agatccgggt gaccgaaata
    57901 gcgacgctga tcggtacgca tgacctgata atgttcggac tgtatcggca acgtgtcagc
    57961 gccgacaata cagccattag aaaaactgcc tatcgattgt gcgctacccg gcacaggttg
    58021 ggttattttt tgccacggcg tcgctgccag gctagcacta ctggcaagca gagccagcag
    58081 cgcaatcgcg gttttgttca ttttttacca gcgtggaata tcagtcttca catcggcatt
    58141 ttgcgcccgc tgccgtaaca ggtgatccat taaaacgatc gccagcatcg cttcagcgat
    58201 cggcactgca cggatcccga cacagggatc gtgacggcct ttggtgatca tctcaacttc
    58261 ttcgccaaag cggttaatag tgcgccccgg cacggtaatg ctggaggtcg gtttcagcgc
    58321 catatgagca atgatttgct gcccgctgct gataccgccg aaaatgccgc ccgcatggtt
    58381 gctctggaaa ccgtctttgg tgatttcgtc gcggttctgg ctgccgcgca gcgccactac
    58441 gtcaaaaccg tcgccaattt ccacgccttt caccgcgttg atgctcatca gcgcatgggc
    58501 gatgtcggca tccaggcggt caaagaccgg ctcgccaagt ccggcgggga caccactggc
    58561 aacaacggtg actttcgcgc cgatggagtc gccctctttt ttcagcgcgc gcatcagttc
    58621 atctaacgcg tcgattttgt cggggtccgg gcagaagaac ggattttgtt caaccagcga
    58681 ccagtctttg atttccagcg gaatgtcgcc catctgggtc aggcagccgc ggatttcaat
    58741 accaaatttc tcggcgagat attttttggc aatcgcccct gccgccacgc gcatggcggt
    58801 ttcgcgggcg gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt tttgttcgta
    58861 ggtgtaatca gcatggcctg gacggaagac gtctttaatt gcgctgtaat cctgagaacg
    58921 ctggtcggta ttttcgatca acaagccaat gctggtgcca gtagtaacgc cttcaaaaac
    58981 accggagagt attttgacct gatccggctc gcggcgctgg gtggtatagc gcgatgtccc
    59041 agggcgacga cgatcaaggt catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg
    59101 aacaccatcg acgatgcagc cgagcgccag cccgtgcgat tcgccgaagg tggttacgcg
    59161 aaagagttgt ccaattgtgt ttccagccat cacggctccg ttattgttgt gtttgcgtgt
    59221 ttacttaatc tttataaatc gcgaaatgtt ctcgtgcggc aataagctgc tctttagtga
    59281 gcataaacac accatcgccg ccgttatcaa actccagcca ggtgaacgga acatcgggat
    59341 attgttccat aagatgtacc atgctgttgc ccacttcaca aatcaacacg ccatcatcag
    59401 caaggtaatc tgccgcgtta ccgagaatgc gacgcgtcag tttcaggccg tcggtgccag
    59461 atgccaggcc cagttccggc tcgtgacggt attcgtttgg caggtcggac atatcttccg
    59521 cgtcgacata cggcgggtta gtgacaatca gatcgtattg cactttcggc aaatcacgga
    59581 acagatcgga acgaatcgga atgacgttgt ggatcagacc gtgttcttcg atgttctgtt
    59641 cggcaaccgc cagcgcgtcg ggagagatat ctaccgcgtc gacttccgct tccgggaagg
    59701 cataagcaca ggcaatggcg atgcagccgc taccggtaca catatctaaa atatgctgcg
    59761 gttgcttgct gatgagtccg gcaaatttat tgttgatcag ttcaccaatc ggcgagcgcg
    59821 gcaccagcac gcgttcatcg acgtaaaatt catgaccgca gaaccaggct ttgttggtca
    59881 ggtaagccac cggaatgcgc tcgttgacgc ggcggatcac gcgttcaaca atacggtgtt
    59941 tttcgctgga ggtcagacgc gcggtgcgca tatcttccgg gatatccagc ggcaggtaga
    60001 gcgatggtaa caccagctgt acggcttcat cccacgggtt atcggtgccg tgaccgtacc
    60061 agatatttgc cgcgctgaag cggcttaccg accagcgcaa tatgtcctga atggtttgca
    60121 gctcatttac tgcttcatca acgaaaattt tatccacgta ttcctccagg gcatgatcgc
    60181 aataatttcg gcggctagtt tgccatgaag atgacgataa atcagcattc acgcgcggtg
    60241 agtgaggaaa aatacgttta aaacgatcga ttgtgctacg agtcgggtaa actgtaggaa
    60301 aattagaaat agagacagat aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc
    60361 tctgtttcgc cagttgatgg cggggactcg caagattaag caggacacga ttgtccaccg
    60421 accgcagcgt aaaaaaatca gcgaagtgcc ggtgaaacgc ttgatccagg agcaggctga
    60481 tgccagccat tatttctccg atgagtttca gccgttatta aataccgaag gtccggtgaa
    60541 atatgttcgc ccggatgtca gccattttga ggcgaagaaa ctgcgccgcg gcgattattc
    60601 accggaattg tttttggatt tacacggtct gacgcagctg caggccaagc aggaactggg
    60661 cgcgttgatt gccgcctgtc gccgcgaaca tgtgttttgt gcctgcgtga tgcatgggca
    60721 cgggaagcat attttgaagc aacaaacacc gctgtggctg gcacaacatc cgcatgtgat
    60781 ggcatttcac caggcaccga aagagtacgg cggtgatgcg gctttgttgg tgttgattga
    60841 agtggaagag tggttgccgc cggagttgcc ctgaatgctg gcagcaaatg ccggagcagc
    60901 gtaaacgcct tatccggcct acaaatatgt gataattcaa taaattgcaa ttcacttcgt
    60961 aggcctgata agacgcatca gagcaatact acgaacttca gttcattcaa tacatactga
    61021 aaacgacacg tcgaatcaat cggttgccca ctactggtat tcacattcac cacaatatcg
    61081 tgtagtactt tatccgttac cggatgacga gttggcgtaa ccgttaccgt tgatttatca
    61141 caaccgtaag aggtaaaggt taacaattct gataccgcgc ggcagtatgc tcctctgcca
    61201 tcattatcag aaaaattact gccatcacag agtctgccgc cttccatcag gtaggtcgaa
    61261 agcgtggtgg tcactgttga ccccgtggac tgaattcgta acgccttatt atggctgaca
    61321 taattttcac tccaggagat atcgtatgaa gcgaccggta tcgttgggca tattgtgtta
    61381 tgcactgtag gggccgtata gcttccaaat ccgggtaaat ctgtatagga ataaaaggat
    61441 gcgatccaag ggcaaccata atcatcagat gcaacggtaa ttttaaacgt catatatgca
    61501 caacgaaaaa ttttcgtatc tatgccaggg caatttgagt tattaattgt atcaaagtca
    61561 gtcagtacca aagttggagc tgaacccgac ccagaccagg aaataacccg caacgtatcg
    61621 cctttatacg caaaagaatc atatttccca tatgcaacct cctttggcgt tatcaccgta
    61681 acattgaaaa cactgctcgc tacagcaccg gttaccttgt ccatggcata ataaactctc
    61741 acactgtcaa aactcgcact cataagttta tctccagcat aactcttaga tgcgccgaca
    61801 acactcagaa cgagcagcat taggctcgcg aaaattatgg tccatttttt catttcagaa
    61861 atactcaaag gttgccgtca ctgtggcact aaagtctccg gacgttacat ctcgcccgct
    61921 ctttgcctgt aaccaggtat taaaatttag gctgttattt ccctgattca gcgggagcgt
    61981 gacgcctgtt gtattgttga ttggcaccga tgtgcctgcc gcagtttcaa taccaatccc
    62041 agcgccggat gctgatgacg aggagtcaaa ggccagaaag ccgggtagag cactatcttc
    62101 tgttcctgtc aacgttactt tgacattaaa caatgtagaa ctatgacaat ctttcaattg
    62161 aaacaccaat ggcactcgct ctgactgccc aaggttcatt aaatcactgg cggcaatggc
    62221 aggaaaatgt acttctgcca actggctacc attaatcact ggcgtacacg atttactgat
    62281 taaattgccg gtaaagtgca aattatctcc tgctattgtg gttccactgc ccagcaacat
    62341 caggctacta ataaaaaaca acgtacgctt tttcttcatt ggtaatctac ctgtaacgtg
    62401 gcccaggcct caaagtccgc ctctggcaga atgacaccac tcttttttac tggcacagcc
    62461 gttaacaccg gtaaatcggt ttcattcacc acaagtggcg tattgatcgt aaaactctgc
    62521 cctgcctgtt tgagctgaat gcctaaacca gtgatattgc tgcttactgc agcattatca
    62581 aaatcgcttg gtgtgccact ccagctcaag gtcatttcca ttaatgggtc gcgaaccgcc
    62641 gtatcgcagg taatggaata cggcacggtt tgctcataat tgctgccatt aatgtcatca
    62701 ataagcacat cctgaaaagt tatttcaatg gtttgagcgt tgctaatcgt gcagttaggc
    62761 ggctgcacta aataaccgtg aaaagagacg tttgagagtg ccatcgagca aaagccccac
    62821 agcaaaataa gcgagatccg tttcattggt aatccaccac aattgtggca ctggcgttaa
    62881 attcagctgc cgctaacgtt gtaccactct gtttcaccgg cacagcttcc agaacgggaa
    62941 ctcccgagga aaaattaaat ggcaaccatg agccagaggt taagtcgagt attgtgtgat
    63001 cactactttt ctgaacccga ataccaaacc caggaatacc cgtgctgata actgtttcac
//
LOCUS       DAJTSF010000011        60419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000011 DAJTSF010000000
VERSION     DAJTSF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.011"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..322
                     /locus_tag="OO335_000762"
     CDS             <1..322
                     /locus_tag="OO335_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6001257.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="OO335_000763"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="OO335_000763"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6001258.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="OO335_000764"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="OO335_000764"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6001259.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="OO335_000765"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="OO335_000765"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6001260.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="OO335_000766"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="OO335_000766"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6001261.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="OO335_000767"
     CDS             complement(3726..4955)
                     /locus_tag="OO335_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6001262.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="OO335_000768"
     CDS             complement(5019..5459)
                     /locus_tag="OO335_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6001263.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="OO335_000769"
     CDS             complement(5564..6334)
                     /locus_tag="OO335_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6001264.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="OO335_000770"
     CDS             complement(6331..7257)
                     /locus_tag="OO335_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6001265.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="OO335_000771"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="OO335_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6001266.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="OO335_000772"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="OO335_000772"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6001267.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="OO335_000773"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="OO335_000773"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6001268.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="OO335_000774"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="OO335_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR6001269.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="OO335_000775"
     CDS             12964..13311
                     /locus_tag="OO335_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6001270.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="OO335_000776"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="OO335_000776"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6001271.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="OO335_000777"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="OO335_000777"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6001272.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /locus_tag="OO335_000778"
     CDS             complement(15131..15493)
                     /locus_tag="OO335_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6001273.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="OO335_000779"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="OO335_000779"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6001274.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="OO335_000780"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="OO335_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="OO335_000781"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="OO335_000781"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6001275.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="OO335_000782"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="OO335_000782"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6001276.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="OO335_000783"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="OO335_000783"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6001277.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="OO335_000784"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="OO335_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6001278.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="OO335_000785"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="OO335_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6001279.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="OO335_000786"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="OO335_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6001280.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="OO335_000787"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="OO335_000787"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6001281.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="OO335_000788"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="OO335_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6001282.1"
                     /translation="MPPLRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="OO335_000789"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="OO335_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6001283.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /gene="tadA"
                     /locus_tag="OO335_000790"
                     /pseudo
     CDS             32769..33916
                     /gene="tadA"
                     /locus_tag="OO335_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="OO335_000791"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="OO335_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR6001284.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="OO335_000792"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="OO335_000792"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6001285.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="OO335_000793"
     CDS             complement(36348..36386)
                     /locus_tag="OO335_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001286.1"
                     /translation="MIIKTLKMSARK"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="OO335_000794"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="OO335_000794"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6001287.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="OO335_000795"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="OO335_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6001288.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="OO335_000796"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="OO335_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6001289.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="OO335_000797"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="OO335_000797"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6001290.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="OO335_000798"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="OO335_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6001291.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="OO335_000799"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="OO335_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6001292.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="OO335_000800"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="OO335_000800"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6001293.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="OO335_000801"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="OO335_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6001294.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="OO335_000802"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="OO335_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6001295.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="OO335_000803"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="OO335_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6001296.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="OO335_000804"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="OO335_000804"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6001297.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="OO335_000805"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="OO335_000805"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6001298.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="OO335_000806"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="OO335_000806"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6001299.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="OO335_000807"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="OO335_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6001300.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="OO335_000808"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="OO335_000808"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6001301.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="OO335_000809"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="OO335_000809"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6001302.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="OO335_000810"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="OO335_000810"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6001303.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="OO335_000811"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="OO335_000811"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6001304.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="OO335_000812"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="OO335_000812"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6001305.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="OO335_000813"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="OO335_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6001306.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="OO335_000814"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="OO335_000814"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6001307.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="OO335_000815"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="OO335_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6001308.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14472 a        16730 c        14848 g        14369 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accgaccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ctccttgatg taaacaaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tctccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgtctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcaac aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 tgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gccccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggtaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcga attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga gtccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcacatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc attggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgagcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgat
//
LOCUS       DAJTSF010000012        58030 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000012 DAJTSF010000000
VERSION     DAJTSF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.012"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(24..278)
                     /locus_tag="OO335_000816"
     CDS             complement(24..278)
                     /locus_tag="OO335_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR6001309.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="OO335_000817"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="OO335_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6001310.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="OO335_000818"
     CDS             1228..2694
                     /locus_tag="OO335_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6001311.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="OO335_000819"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="OO335_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6001312.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="OO335_000820"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="OO335_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6001313.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="OO335_000821"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="OO335_000821"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6001314.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="OO335_000822"
     CDS             5709..7265
                     /locus_tag="OO335_000822"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6001315.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="OO335_000823"
     CDS             7347..9161
                     /locus_tag="OO335_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6001316.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="OO335_000824"
     CDS             9162..10256
                     /locus_tag="OO335_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6001317.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="OO335_000825"
     CDS             10256..11281
                     /locus_tag="OO335_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6001318.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="OO335_000826"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="OO335_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6001319.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="OO335_000827"
     CDS             complement(12876..13220)
                     /locus_tag="OO335_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001320.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO335_000828"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO335_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR6001321.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO335_000829"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO335_000829"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6001322.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="OO335_000830"
     CDS             15616..17376
                     /locus_tag="OO335_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6001323.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="OO335_000831"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="OO335_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6001324.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO335_000832"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO335_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6001325.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="OO335_000833"
     CDS             19113..19340
                     /locus_tag="OO335_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6001326.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="OO335_000834"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="OO335_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6001327.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="OO335_000835"
     tRNA            21195..21271
                     /locus_tag="OO335_000835"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO335_000836"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO335_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR6001328.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="OO335_000837"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="OO335_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6001329.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO335_000838"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO335_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6001330.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO335_000839"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO335_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6001331.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO335_000840"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO335_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6001332.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO335_000841"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO335_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6001333.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO335_000842"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO335_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6001334.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO335_000843"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO335_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6001335.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO335_000844"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO335_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6001336.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO335_000845"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO335_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6001337.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO335_000846"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO335_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6001338.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO335_000847"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO335_000847"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6001339.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO335_000848"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO335_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6001340.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO335_000849"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO335_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6001341.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO335_000850"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO335_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6001342.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO335_000851"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO335_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6001343.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO335_000852"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO335_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6001344.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="OO335_000853"
     CDS             37391..37555
                     /locus_tag="OO335_000853"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001345.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="OO335_000854"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="OO335_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6001346.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38300..39331)
                     /locus_tag="OO335_000855"
     CDS             complement(38300..39331)
                     /locus_tag="OO335_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR6001347.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39565..39924)
                     /locus_tag="OO335_000856"
                     /pseudo
     CDS             complement(39565..39924)
                     /locus_tag="OO335_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="OO335_000857"
     CDS             complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="OO335_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6001348.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="OO335_000858"
     CDS             complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="OO335_000858"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR6001349.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42511..43575)
                     /gene="ada"
                     /locus_tag="OO335_000859"
     CDS             complement(42511..43575)
                     /gene="ada"
                     /locus_tag="OO335_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6001350.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="OO335_000860"
     CDS             complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="OO335_000860"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6001351.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="OO335_000861"
     CDS             complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="OO335_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6001352.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46675..49347
                     /gene="rcsD"
                     /locus_tag="OO335_000862"
     CDS             46675..49347
                     /gene="rcsD"
                     /locus_tag="OO335_000862"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6001353.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49364..50014
                     /gene="rcsB"
                     /locus_tag="OO335_000863"
     CDS             49364..50014
                     /gene="rcsB"
                     /locus_tag="OO335_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6001354.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="OO335_000864"
     CDS             complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="OO335_000864"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6001355.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53338..54114)
                     /locus_tag="OO335_000865"
     CDS             complement(53338..54114)
                     /locus_tag="OO335_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR6001356.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54119..55768)
                     /locus_tag="OO335_000866"
     CDS             complement(54119..55768)
                     /locus_tag="OO335_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6001357.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55769..56328)
                     /locus_tag="OO335_000867"
                     /pseudo
     CDS             complement(55769..56328)
                     /locus_tag="OO335_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56487..>58030)
                     /locus_tag="OO335_000868"
     CDS             complement(56487..>58030)
                     /locus_tag="OO335_000868"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6001358.1"
                     /translation="TSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNN
                     VRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQ
                     GKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKP
                     AAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQN
                     GQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRN
                     NLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGP
                     GARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLL
                     HRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPL
                     ADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTA
                     AILMV"
BASE COUNT        14195 a        15654 c        14259 g        13922 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgcg ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccatgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgagg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ttttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggcc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gttaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc atgatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatcat tacaacgcaa tatccgccac ttctttttga acgggttgcg
    38341 gtttcaactg cggtttcggc gtactatctg ctgcttgcgg tttgtcgtat ttcagcccca
    38401 gcacttcgct ggtgtactgc aactcgcgtt ctgttgccgc tacatcaccg ttcagcttgc
    38461 gtccataaga cggaacgatc gctttcaacg tagcctgcca ttgcgggctg gaaacgcgat
    38521 cgccaaatac tttttccagt agattcaaca taatcggcgc ggcggttgac gcccctggcg
    38581 atgcccccag cagcgccgca atggtccctt gctggtcact gaccacttcg gtacccagac
    38641 gcagtacgcc acctttctcg gcatcacgct tgataatctg cacacgctgc cccgcttgcc
    38701 acaaacgcca gtcctctttt ttcgcttgcg gatagtactc tttcaacgct tcaaaacgat
    38761 cctcttcact caacatcacc tgactcacca gatatttcac cagatcgaaa ttatccagcc
    38821 cgacgtgcat catcggcatc acgttagagg tggtggtgga actcatcaga tcccacaatg
    38881 aaccgttttt gaggaatttg gttgagaagg tggcaaatgg cccaaacagc actacgcgtt
    38941 taccgtccag aacacgggta tcgatatgcg gaaccgacat cggtggtgcg ccaacggatg
    39001 ctttaccgta aactttcgcc agatggcgat taaccacgtc tggattttcc gaaacaagga
    39061 actgtccgcc caccgggaaa ccggcgtagt ctttcgcttc cggaatcccc gattcctgta
    39121 acagcttcag cgccgcaccg cccgcgccga taaagacaaa tttggcgcgg atgttctgtg
    39181 cagtgccgtt tttcagatcg gcaacggtaa cggtccaggt gttatcgtca ttacgcttta
    39241 gggcgcggac ttcgctgctg agttgcagcg agaagttaga tttcttctgt aaggaagcaa
    39301 ttaactggcg ggtgatctca ccatagttca catcagtgcc aatttctgtt gcggatcgcg
    39361 cccttccatc actaacggtg cccactcttt gatctgcgcg tgatcttcag agtaacgcat
    39421 accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg gcgcgcagga aattaacgtt
    39481 atcctcgccc cagacaaagc tcatatgcgg aacggtattg ataaatgaac gcggagtacg
    39541 cagcacgccg cgctcgacct ggtgcgccca gaactggcgg gaaatctgaa atgcttcgtt
    39601 aatggcgact gctttttcaa tactgatgct gccatcggcg ttttgcgggg tgtagttcag
    39661 ttccatcagt gcagaatgcc cggttccggc gttgttctag ccgttcgaac tctcctgcgc
    39721 gcccccctcc aggcgctcca ccatggtcat cgaccattca ggctccagct cgcgtcaata
    39781 ggtccccaac gtggcgctca taatgccgcc gccaatcaac agtacatcag tttcctgctc
    39841 ctcggacgct ttcgcttttg ccgccatcga aacggcatta agccccacgg ccatcgagaa
    39901 gagcatggca gtcacttttt tcatcttgtt aatgccttac ttttagtcgc tttattgcag
    39961 gtgagatttg cgcggcatca acggtaacac ttaagtaaca acatttaaat aatgtttata
    40021 aattatattc taattttaga aacattataa tttcgttgat taattatagg gttattagaa
    40081 atgaagggat ttttagcata ttgacctgac ggcagcagtc cgccaggtca gcgtcgtaat
    40141 attatgccgt ccgggcaacg gcatcacgcg aagcggcatc gcgctcttcg cccgtcagct
    40201 cgctaagttg cccattgcgc atttccagca ggcggtcggc gtggataaag taatgatcat
    40261 catgactgat agcgaaaata gttttaccca tctcctgcat cagcggcagc aacacctgat
    40321 aaaactcacg acggaagtgt ggatcctgat ccaccgccca ttcatccagc aggataatat
    40381 cgcgttcttc tgccagcgcc agcaacagcg ccacgcgttt tttctgccct tttgataact
    40441 tcaggttaac aatacgcccg ttgcttaact caagcttatg agccattttc agctgcgcca
    40501 gccacttctc aaccagttgc gggttagcgg gttgcccctc cggccccagc agttgatcaa
    40561 acagccagac atcggtaaac actgccgaaa acagtttgcg ataatcttcc ggttgttcgc
    40621 cgctgacagg tttgccatcc agcaagattt cgccgctttg tggctgatac aagcccgtca
    40681 acaacatcgc cagcgtcgat tttccgctac cgttaccgcc aatcagaaac agcagctcgc
    40741 cacgtttgat ggtgagatta atcggaccaa cggaaaacgc gttatcctga taagcaaacg
    40801 tcacgttacg cagctccagc gtttgccagt tgggaaacgc ctgcgggcgc ggaaactcag
    40861 ctttgaaagg cgcgagcgcg aatttgttca gcttgttaaa cgccacctgc gccgtcagca
    40921 gcgtcggcaa tgcgccaacc gccgaaagca gcggcgtacg caggaataac agcgtcaacg
    40981 aataggtcgc agcaacgttg gtatcagccc aaccgagact gttcgccatc cagaacacca
    41041 ggccgatagc gcccagcatc atgatgtttg accagttcac ggcactaaga tggaaggtgt
    41101 ctgcgcgaat aatatgatgg cgatactctt gcgcatcggg gatgtagagg ttgttaaaca
    41161 catactcggc gcgttcccgg ttcagagtca gctctttgcg cccttcaagt acagtttgaa
    41221 aatccgtgta cagcttgtct tcggtttcac gtagggtcgc catatgtttg tacacccgcg
    41281 ccaccagcac aaaaccgccc cagatggtga tcgccatcca gatagccgtt accagcaaca
    41341 ttttgcccga cagcatccac agatacgccg atgaaccgat agtgagaatg atcccctgcc
    41401 ccagttccgg cagacgcaca aaagcaatgg tgatattgcg cacatcgctg gttaatcccg
    41461 ccagcaacga ggcgctaccg agttgttcaa tgcgctcgac gtgggtatcc agaatccgct
    41521 tgataaattc actacgcagt cggtaaacga agtgatgccc caaagtggtg agcgccagtt
    41581 gcgatccgag agtgactgcc atcaacagca gcaataatcc cagaaactcc ggtaacacca
    41641 gcagactggt atccgccgtt tcgataaggc gctgattgat aaaagcaatt aagccaatgc
    41701 ctaatgccgc actggcgagg cttagcgcca tcacactgat aaatggccag cgatactgcc
    41761 gccagacaag tacaagaagt tccatgcaga aaacccggac aatgaattac agcccgcagt
    41821 ttaaacatct tcgcgcgcac agcaataata attcttattt ttattctttt ttacctgcct
    41881 gacggaatgt caggttgtag cggcagtcgg tggtgagtgg atgaaacccc gctttcaacg
    41941 gttgaatacc gtgataaaac agccgcgatt caccgcccca taccaccaca tcgccatgtt
    42001 ccaacaacaa acgtttgagc ggatcattac gtttcaggcc accaaattga aaaatcgcgg
    42061 gtaagcccag agaaacagaa acaattggcg cgcgcagatc cggttcgtct ttatcctgat
    42121 gcagcgacag tttcgcgcca ggagcgtagc ggttgataag acaagcatct ggctggaaat
    42181 ctggatagcc cgccgccgta gccgcacgtt gacataaatc atgaaagctt tgtggcatag
    42241 cgggccacgg tttatttgtt tgcggatcaa tgggcgaata gagataacct tgccgatggg
    42301 tcgtccagcc cagatgccca cagttggtca tcgccaccga catggtatat ccgcctggag
    42361 cgaccatctg gcgaaacggc gactggctgg caacgtcatt aatatcgcgg attagttgct
    42421 ccgcagcatt aaaagcaaaa cgccgtaaaa ttaccgcacc agccgccagt ggctcttgcc
    42481 acggttcagc atcggcaaac agatccaaca ttacctctcc tcattttcag cttcgcggcg
    42541 cagcagttgc gctttacgcg acacgcccca gcggtaaccg gaaagtgtgc catcaccacg
    42601 gaccacccga tgacagggta ttacgatagc cagcttgttg gcggcacagg cgctggcaac
    42661 ggcccgtacc gctttcggtt tgccgatggc gttagccagt tgctgataac tgacggtttc
    42721 accacaaggt atcgtgcgca gtgcctgcca gacttgttgc tgaaaagcag tgccgcgaat
    42781 atccagcggt aacgtcagcg gcgtatcgcg ttgattgagg ctggcgatca cttcacgcac
    42841 atgttgctga aacgtcagat cggcaggcgc gttgtcggca gcgggaaaca tctgctgcaa
    42901 ctcgctgatt aatgtcgcgt catcatcgcc cagcaatatc gcgcaaatcc cccgctcgct
    42961 ttctgccacc aggcaacgac ccagctcaca atcagccagc gcgtaacgca ccgccagatt
    43021 ttcgccaccg tgacggaatt gtttagccgt catgcccagc gtttcatcgg ctttgcggta
    43081 gtaactgctg ctgtcgggga atccggcgtt aagaatagac gtcgtcacgc tctccccttt
    43141 cgccagcgat tcgcgcaaac ggcgagcgcg ccaggcctgt tgccaggctt taggcgtcat
    43201 tccggtagta gctttaaaca accgatgtag atgaaatgga ctcatcgcca cctggtcggc
    43261 taaggcttcc agcgttacag gcgtttcctg ttccagcagt cgacacgcgt gggtgatttt
    43321 atccaaccga tgttgccggg gattggcttt gtctggctga caacgtttgc aggggcgaaa
    43381 tccggcggcg agtgcctcgc tggcatttgc gtagaaggag acattttccc gcaaggcatg
    43441 tctggcgcgg caagaaggac ggcaaaagat acccgtggtg cgcacggcga aaacgaattc
    43501 gccgtcggca ttcgggtcgc gggctaagac agattgccag cgttgatcgt cagttaagca
    43561 tgtggcgttt ttcataatca gctccctggt taaggatagc ctttagtctg cccggtcacc
    43621 atcacgcaaa aaccaacaat cttgcgcttt aatttttttc gctgacaagg aagcttttaa
    43681 actgtggtga catccaggtt ttaaagctat cgccttcttt ggtgatcata taaaccgcca
    43741 gcccttcccg gcgaacaacc tctttggctt tctccggccc gagtaccatc aagccagtat
    43801 cccaggcatc ggcttccagc gccgtcggag caatcaccgt cacggatacc agattgtgtt
    43861 cgatgggacg cccggtttgc ggatcgataa catgggaaag acgtttgccg tccagttcgt
    43921 aatagttacg gtagctgcca gaggtgctaa tcccatgacc gttgatatcc accacagcct
    43981 gaaccgcgtt ttctttatcg gttggttttt gaatcgctac ccgccacggc tggccttcac
    44041 cgttcatacc acggctgttc agcgcgccgc ccaccgacac cagatagcgg gaaatccctt
    44101 cctgctccat caagcgtgcc aggtgatccg ccgcataacc ttcaccgacg gtggagagat
    44161 cgacatataa atccggcagg tctttttgca gatattgctg atgcgactga ttgatgaccg
    44221 tcaggtgctg taagccggtt ttggctttca tcgcatcgat ctgttcctgg ctcggaattt
    44281 gaaccggctg ttgttccggg ccaaagcccc acagattcac cagcggcccg acggtaatat
    44341 ccatcgcacc atcggtcctt gccccaatgc gcagcgaggt ggtgacgata tcggccatcg
    44401 cttcacttac cggccacggc gacaaacttt gcgagtcgtt aaagcgcatc agcgcggaat
    44461 cttttttata ggtcgaaagc agctgatcgt cggcgtccag ctgggtctga atcttttctt
    44521 taagttcggc actgcgtttg gcatcaatgc ccgggatgct ggcacgccag aaggtaccca
    44581 tggttttgcc ttcaagaacg gtaacttcag tagcgtgggt tttggcgggt tgtggttttt
    44641 gatcgcaacc aacaaagaag agcgcggcaa ccagcagtgc cacgcgggta aagcttattt
    44701 ccatacgtga ttatcctcat gcgaacggtc gcaagagtac accaaaaaac tgtgtttgta
    44761 cgctgaaaac aatgaaaaaa gggcccgcag gccctttgtt cgatatccat cgagattaga
    44821 actggtaaac cagacccaga gctacgatgt tatcagtgtt gatgccagcg tcacgagtga
    44881 actggttgtc gtccagcagg ttgattttgt agtcaacgta ggtggacatg tttttgttga
    44941 agtagtaggt cgcaccaaca tcaacatatt tcaggatatc ttcgtcgtcg tagttacgac
    45001 cattgatgac acccaggttt ttacctttag actgcaggta agccagggac ggacgcagac
    45061 cgaagtcgaa ctggtactga gcaacagctt cgaagttctg tgctttgttc gcccaaccca
    45121 agttgcctac gcgagttgcg ttgtaggtct gggtgtactg agcagctagg tagatgttgt
    45181 tcgcgtcgta tttcagacca ccggtgtagg tttcagcgcg gtcgccatta ccgatgtaac
    45241 gctcatcata acggttcaga ccgaagttct ggtcatcagt acgtttggag ctggaaactg
    45301 cagcaccgat accgaagcct tcgtaatcat aagtaataga accgccaacg ccgtcaccgt
    45361 tctgacgcag agcttcacga ccgttgttgg tcatgccttc accttccggg ctaccgtttt
    45421 tgccctgata ctgaacagcg aagttcagac cgtcaaccag accaaagaag tcggtgctac
    45481 ggtaggttgc gaagccgtta ccacgctgct gcatgaagtt gtcagaaccg taagtgtcac
    45541 caccgaattc tggcagtacg tcggtccagg aagttacgtc gtaaactacg ccgtagttac
    45601 gaccgtagtc gaaagaacct acatcctgga atttcagacc tgcgaatgcc acacgggtcc
    45661 aggagttgtt ttcgttttca gcgctgttgc cctggatctg atattcccac tggccgtaac
    45721 cggtcagctg gtcagtaacc tgagtttcac ctttgaagcc aagacgcatg taggtctggt
    45781 cgccatctac agacttgtcg tcagagaaat agtgcaggcc gtctacttta ccgtacagat
    45841 ctaatttgtt gccgtctttg ttgtaaactt cagcagcgtt tgctgcgcct gctaccagca
    45901 gagctgggac caggagggac agtactttaa ctttcatgtt attaaccctc tgttatatgc
    45961 ctttatttgc ttttttatgc cactgcatac tgattaaccc tcattaatca gtcggcaagt
    46021 ccattctccc caaaaatgca gaataatcca acacgaatat gatactaaaa cttttaagat
    46081 gtttcattta tcgctataga tgtttcaaaa tgtaaataca agggaacttt ttaagattat
    46141 tgcggaatgg cgaaataagc acctaacatc aagcaataat aattcaaggt taaaatcaat
    46201 aacttattct taagtatttg acagcactga atgtcaaaac aaaaccttca ctcgcaacta
    46261 gaataactcc cgctatcatc attaacttta tttattaccg tcattcattt ctgaatgtct
    46321 gtttacccct atttcaaccg gatgcctcgc attcggtttt tttacccttc tttacacact
    46381 tttcattatt ctgtgctacc acagaaaaac tataacgctt gttaactatt tcacaaataa
    46441 ttaacatcag cataatttcc agcaatcttt gtttatttgc aattaatttc gttgggcttt
    46501 ttgtaggtta tttgtacagc aaaatggcgc ttgtacatct atttcccccc atacaggatg
    46561 ataaatatca cgggagaata gagaatcatc aatcaggtaa gagtctggaa tttcacactg
    46621 taccctttat actgccctat cacttcgcga agttttaaca ggtcataaac acgaatgcgt
    46681 cagaaagaga caacggccac gacccgcttt tcactcctac cggggagcat tacccgcttc
    46741 tttttactgt tgatcattgt gttactggtg acgatggggg taatggtaca aagtgccgtt
    46801 aacgcttggc tgaaagataa aagttaccag attgtcgaca ttacccacgc aatccaaaag
    46861 cgcgtcgata cctggcgtta cgtgaccagg cagatctacg acaacattgc cgcgacgacc
    46921 tccccctcct ccggcgaagg tttacaagag acgcgcctga aacaggatgt ctactatctg
    46981 gagaaaccac gccgcaaaac ggaagcgtta atctttggct ctcacgacaa ctcaacgctt
    47041 gagatgactc agcggatgtc cacttatctg gatacattgt ggggtgcaga aaatgtaccg
    47101 tggtcgatgt attacctgaa tggtcaggat aacagtctgg tgctgatctc aaccctaccc
    47161 cttaaagatc tcacctccgg atttaaagaa tcgaccgtca gtgacattgt tgattcacgt
    47221 cgtgcagaaa tgttacaaca ggccaacgcc ctcgatgaac gcgaaagttt ttctaacatg
    47281 cgccgcctag cctggcagaa cggtcattac tttaccttgc gtaccacgtt caaccagccg
    47341 ggacatctgg caacggtcgt ggcttttgat ctgccgatta atgatttgat cccaccgggt
    47401 atgccgctgg acagtttccg ccttgagcca gacgcgacgg caacgggaaa caatgataat
    47461 gagaaagaag ggacggatag cgtcagtatc cactttaaca gtacgaagat tgaaatctcc
    47521 tcggcactca actctaccga tatgcgcctg gtctggcagg ttccttatgg caccttattg
    47581 ctggatacgt tgcaaaacat tctgctgcca ctgctgctga acatcggttt gctggcgctg
    47641 gcgttatttg gctataccac attccgccat ttctccagcc gcagtacaga aagcgtcccc
    47701 agcacggcgg tcaataacga attgcgcatt ttacgggcaa tcaatgaaga gatagtctca
    47761 ctgttgccgc tcggcctgct ggttcacgat caggaatcga accgcactgt cataagtaac
    47821 aaaattgccg atcatttgct gccgcatctg aatctgcaaa acatcaccac catggcggaa
    47881 cagcatcagg ggattattca ggcgacgatc aataacgagc tgtatgagat ccgcatgttc
    47941 cgcagccagg tcgcgccgcg cacacaaatt ttcattattc gcgatcagga tcgcgaagtg
    48001 ctggtaaaca agaaactcaa gcaggcgcag cgtctgtatg agaaaaacca gcaggggcgg
    48061 atgaccttta tgaaaaacat tggcgatgcg ctgaaagaac ccgcacagtc cctggcggag
    48121 agtgcggcta aactcaacgc cccggaaagc aaacaactgg cgaatcaggc agatgtgctg
    48181 gtgcggctgg tcgaagaaat acagttagcg aacatgcttg cggatgatag ctggaaaagt
    48241 gagacggtgc tgttctccgt gcaggattta attgatgaag ttgtgccttc agtgttgcct
    48301 gccatcaagc gtaaaggtct gcaactgctg attaacaatc atctgaaagc acacgatatg
    48361 cgccgcggcg atcgcgatgc cttacgacgt attttgctgc tactgatgca atatgccgtg
    48421 acctcaacgc aattgggaaa aatcaccctt gaggttgatc aggatgagtc ctctgaagac
    48481 cgcctgacat tccgcattct ggacacggga gaaggcgtaa gtattcatga aatggataat
    48541 ttgcacttcc cgtttatcaa ccagacccaa aacgatcgtt atggaaaggc ggacccgctg
    48601 gcattctggc tgagcgatca actggcacgt aaactgggcg gtcatttaaa catcaaaacg
    48661 cgggatgggc ttggtacacg ctactctgtg catatcaaaa tgctcgccgc tgacccggaa
    48721 gttgaagagg aagaagagcg tttactggat gatgtctgcg taatggtgga tgttacttcg
    48781 gcagaaattc ggaatattgt cactcgcctg ttagaaaatt ggggtgcaac ctgtatcaca
    48841 cccgatgaaa gattaattag tcaagattat gatatctttt taacggataa tccgtctaat
    48901 cttactgcct ctggcttgct tttaagcgat gatgagtctg gcgtacgaga aattgggcct
    48961 ggtcaattgt gcgtcaactt caatatgagc aacgctatgc aggaagcggt cttacaatta
    49021 attgaagtgc aactggcaca ggaagaggtg acagaatcgc ctctgggcgg agatgaaaat
    49081 gcgcaactcc atgccagcgg ctattatgcg ctctttgtag acacagtacc ggatgatgtt
    49141 aagaggctgt atactgaagc agcaaccagt gactttgctg cgttagccca aacggctcat
    49201 cgtcttaaag gcgtatttgc catgctaaat ctggtacccg gcaagcagtt atgtgaaacg
    49261 ctggaacatc tgattcgtga gaaggatgtt ccaggaatag aaaaatacat cagcgacatt
    49321 gacagttatg tcaagagctt gctgtagcaa ggtagcctat tacatgaaca atatgaacgt
    49381 aattattgcc gatgaccatc cgatagtctt gttcggtatt cgcaaatcac ttgagcaaat
    49441 tgagtgggtg aatgttgtcg gcgaatttga agactctaca gcactgatca acaacctgcc
    49501 gaaactggat gcgcatgtgt tgattaccga tctctccatg cctggcgata agtacggcga
    49561 tggcattacc ttaatcaagt acatcaagcg ccatttccca agcctgtcga tcattgttct
    49621 gactatgaac aacaacccgg cgattcttag tgcggtattg gatctggata tcgaagggat
    49681 cgtgctgaaa caaggtgcac cgaccgatct gccgaaagct ctcgccgcgc tgcagaaagg
    49741 gaagaaattt accccggaaa gcgtttctcg cctgttggaa aaaatcagtg ctggtggtta
    49801 cggtgacaag cgtctctcgc caaaagagag tgaagttctg cgcctgtttg cggaaggctt
    49861 cctagtgacc gagatcgcta aaaagctgaa ccgcagtatt aaaaccatca gtagccagaa
    49921 gaaatctgcg atgatgaagc tgggcgtcga gaacgatatc gccctgctga attatctctc
    49981 ttctgtgacc ttaagtccgg cagataaaga ctaatcatct gtaggccaga taagacgcgt
    50041 tagtgtctta tctggcattt gcaccgattg ccggatgcgg cgtaaacgcc ttatccggcc
    50101 tacgattccc attatttcaa cagattacat taaagtaggc cagataagac gcgtcagtgt
    50161 cttatctggc atttgcactg aatgccggat gcggcgtaaa cgccttatcc gtcctacgaa
    50221 tcccgcgatt tcctgaccct ctcggcatat accgtcagcg tctgttttat cacatccagc
    50281 gttaccggct tcgagaggca gctgtccata ccggactcca gacaccgctg cttctcttca
    50341 gccaacgcat tagcagttac tccgattacc ggcaacgtca gtcccaactg acgaatgcgt
    50401 tgcgtcaagc ggtaaccatc catatttggc atgttgacgt cgctaagcac gatatcaata
    50461 tgattcttac taagtacatt aagcgcatcg acgccatcat tcgcggtttt acattgatag
    50521 cccaacgatc ccaactgatc tgccagcaga cgtcggttaa tcggatgatc atccacgacc
    50581 agaatcatca tatcgtcgtt atcgctgacc gctttgtccg tcgacggcag agcgttagca
    50641 ggatcgtcgc tctccatctc gatcaaataa atacgcgcca acaatgccgg tagctcatgc
    50701 ggagcagcca cactgtgtac ccactcccct ggcgctttct ccagcggaat accaatatgg
    50761 cgacgacaga aggtcactac cgctctgccc tgccattttt tactcactac ctcgtcagtg
    50821 attaacacat cttcgggagt cggttcctgc ccttcgtatg ttgtaacgac gatgccgctg
    50881 cgctgcaaac tggtttccag gaactgacag agcgacgcat tgcggaccgc cagccagcag
    50941 cgtttaccac tcaacccttc cacgcctttt ttctgcgggt actgagcgcc gtacaacgga
    51001 atacgcacgg taaactggct gcccattccc ggttctgaat ctaccgagat atcgccgtcc
    51061 atcatgctga tcagtttttc acaaatcgcc agacccagac cggtcccctg gaagtttcgc
    51121 tgtacgcccg ttccaacctg gaagaaggga tcaaacaagc gcaccacttc tttcgccgga
    51181 atccccacgc cggtatcgcg aacacggata gagagataat cgccatcagc gcgaacatgc
    51241 aaaactatac agccggtatc ggtgaatttt atggcgttac tcaacaggtt ggagatgacc
    51301 tgctgtaaac gcatcgggtc gccatttaag gccactggca catccggttc aataaagcag
    51361 tacaagccta actgcttgcg taccaccagc ggtaaatagt tggcggtgat gtggttcatc
    51421 acttcacgcg gtgaaaactc acgcggttcg atcttcaact gttccgattc aatcttcgag
    51481 aaatcgagaa tatcgctgat aattttcaac aacaggctgg aagagttgtt cattgccgtc
    51541 accagacgat cgacgccttt cggtaactct ttggtttgca acagatccag gttaccgata
    51601 atgccataca gcggcgttcg cagctcatga ctgacggtgg caaggaacat cgattttgac
    51661 tggctcgcct gttccgctgc ttgtgccatc tcctgcaacg actcttccat cttcacgcgc
    51721 gaagaaacat ccaccagcac acaaatggcc acgttttcat tacgatagcg cgaatggacg
    51781 aagctgattt gcagattggt attgttgctg gtcaggacat caacaaaatt gacctgctgc
    51841 ccacagataa tttgcgtcag tcgttggcgg tcctcatgcg taagcatatt gagataggta
    51901 tgcgccagtt cgttacttaa aatattgacg ccatcagcgg tacgcaaaat gcagataccc
    51961 actggcgcgg aggcgacaat cttgcgattg aactgctcat gttcttccag tcgcagggcg
    52021 tcgctttccg ccggaatgaa aatacgtcgc tcgtacatcc gcgcgagagt aaacaatgca
    52081 gctccggcaa gcacattcag caaaattgcg ttaaggatca acatgcgaat gcgttccagc
    52141 accttatcaa ccggcaccga atacacgatg cttagcgatg agggtggcag atttttcttc
    52201 agcaccagct cccggaaccc ttccgtatag ccaaaccagg agcgttcctg catccagcga
    52261 ggatcgccct taattttact ttctggtccg gtaagcgaaa tcagggtatg accattttca
    52321 tcaagaatgg taacccccat cggcaacgta cccggtaaga aaaagttctc catccggatg
    52381 gtctgctcga cacccaaaag cgcctgcaac cggttcgcca gataaactgg cgtcaacgcg
    52441 taaaaatacc cgacgcaagg gcgcggacct tcgctgatcc agtagaggtt actgccgcta
    52501 tcatcttgtg gtgcatttcg atatttattg atgcgttcat gcaacgcttt caacgcggta
    52561 tcgcgttcca ctggcatatc acgcagaccg aaattggcca tgcagaggtt atcactgccg
    52621 attaaaaata cccggttgag atcgtaagcc gcagaaaaat tatcgcgcca gtagcgcata
    52681 aaccacgcca atgactccag agaacctcgc caggtgttac tcattgcgga acaatcggag
    52741 tcggcaaaca gcggttcaaa cgcaggcaca tccgcctgcg tttctcgtcc acgcggggaa
    52801 agcacaccgt tttctgccga taagcgattt tcggcgatgt acttaagctc tttcatcaca
    52861 tcagaggtgc gttgaataaa gcgctgagcc tgatcggaac tcagattaaa ttcctgacga
    52921 atttccgatt ctcgctgatg taacgcatta acgatgtaaa aaacggatga aaaagcaatc
    52981 aacagccaga gcactaacgc caatgctctg aacatgtagc gcgaggcttt cagggttgta
    53041 cgaaaagaag caaggtattt caaaggggag aagctccgcc tcaggtgacc gatggagtgt
    53101 ggttaaggta gcggtaaaag cgtgttaccg caatgttctg tcaccattgc aataatgcgt
    53161 atcgcttaga aaccattgcc ttaaccagga aagccggagt agatggacag ttctcagtac
    53221 gaaaaataag aaatgaccaa tctgccgacg cttgttaatg gttatatcct ggcgtcggca
    53281 gatcgcccag cagtcttatc tgaaaaaaga gagagccagg ttgtgtgaag ggatggatca
    53341 ccagtcaaaa cttttcacca gcgtcagctc gccagcatta cgcatcggta caataaatgt
    53401 ttcctgtttc tcattgaccg atccttcatc ggtgatcagc gtcagttggg cggtcgtcaa
    53461 ttccgtttcg ctgcgcccac catagtagtt gatatacacc tgatagcgcc cgtgaactgg
    53521 tgctggcatg gcgaaaatct cgggtccgta ccccgtcgtg acatccatat ccagtgcacc
    53581 actgtttttc agcacggtgt taccgtacca ggcgtgttcg ccatcgggcg tgacaacatg
    53641 gagatcgaga tcggtattgt ccgtatccca cgagagaacc agccgtaaac gtgcacgaat
    53701 agttcctgta cccggcgttg agtaaaactg cattttttgc cggctttgcc catccgggct
    53761 gatgacctgc acgctgttgc tgccttcagt gaaaatataa gggcgtgcaa atgaaccatc
    53821 ggattcaatt ctctgcggca tactggcacc gttgaccacc agccggccct gctgaacttt
    53881 acccgccgca gcataattct ttattttgcc gcggatctgc gctgagatat tttgatcatc
    53941 tgccatgttg acggacgacg ccgggtaatt gatcgattgt gaaaaactgg catcttcgcc
    54001 ctctgccgga tgccagccag agagcggtgc gtcaatctcg acttcctgca caccttcact
    54061 gtgagcgaca ggcgaaagtg ccaccagtaa caacggaaga aaaatctttc gcatagcatt
    54121 agtccattaa taattgtcgg gtcagcgttt cgatatagtt ctcatcaagc ccggtgggat
    54181 aaccatcaaa ggccaagtgc agatactcat gggttaaatc cagccgatcc tgaagcgtaa
    54241 agaagttacg aataaacagc cgtttctgct gccgatcggt ataggggaaa cctgagacta
    54301 aacgacagac cgcaaacacg tctggttcgt tgtaccccgt ctcgccttgt aatatacgcc
    54361 gccactgcgg catttttttc gccagccagg ctttcgcttt gggtaataac tggcaggttg
    54421 agcgtggagc tccccagcgg ctaaggctgt tgtcgggata agcaaacgcg agtatctggt
    54481 cgtaacgctc cccctgcccc gcctgggccg tggcttgccg ccaggaaagt gtcccttcgg
    54541 tagcgcgact gccatgataa tgaaccggat cgcctgcata gatgagctcc tgcgtccagg
    54601 cggtcatcgt tcgcgccccc gtggtcgctg gcgaagcaga aacacgctgc gtggcgctgc
    54661 tgtcggggat ggtcaggcaa tcaccttccc gattggcgtt ttgctgtaaa aaagtacgta
    54721 tcgccacagt catggctttc gctgcctcag gtggcgtact tttcgcctcg cgatccagaa
    54781 ctctggcaac gtactcttca cgatcgagat gcgactgcaa tttcagtttg cccttttcgc
    54841 tcagtaaggt cgtctcaccg tggctgacaa acgtaatgtg gttaccatta gtaaacgtca
    54901 cacgatagcg cccattaagt acgctgggtt ttactgccgt cgtactcttt tccgctgtga
    54961 ttttcttcag cggatagcgg gcaaacagct ccacctctac acattgccca ctggcaacct
    55021 gcgtcggcac tggcaaaacc cgattaagca ccgtcgcata acgggttagt accgttttgc
    55081 tggtgccact gccagtaacc cacagcggtg taccatcggt taaccagcca gcaaaaccac
    55141 cctgacggat ttcctgttta tcatcggcaa accagctcca ggttttcacc cgtacccgac
    55201 taccgagcat ggatgcgaca ccgattttcg cctcgtccag caccacatcc agtaagactt
    55261 cctgagcttt gttttgcgca ggtaacgtcg ctaatgaatc aagcagcgat ttaaccgtta
    55321 ccgaggtttc tggcttcagc gttgtaagag acgctaacca cgcaggtgcc tgacgctgtt
    55381 gccagtattg cccccacaca tctgcgccaa tatgcaaccg ctgcggcgca aaatagagcc
    55441 cacaggaacg taccagcgcg gtatcgcgcg ttatgctctc cccagcctga caacaataaa
    55501 cctcttccgg ggagtttccg cgacactggt agggctgctc cggttgatgg gtatcctcca
    55561 gccaggcgta aacatacagt ttccacaaac tccctaacgg cgtggtgagc gagtcaggta
    55621 atgctgagac tttggtcacg cctgatgacg acagctgata cagttgatcg tgctgcgcac
    55681 cgcgcagcac cagttgtaac ggcgtctcct ccgcaagcgt tggcagagta accaacgcca
    55741 atagccagac gattctgcgc cagttcactt atttcacctg catcccggtc cattcactcc
    55801 ctgccgcgac actttgctgc gcaggtgcat aggaacgcac ataacgagca ggaggcagaa
    55861 cgaattgccc tttttgcgag aagcgcagca aatggcggaa agtgaccgtt cccgtcagtt
    55921 ctttcaccgg caccatatag gccagttcgc ccatttcatt acgcgctttt tccagcaatt
    55981 gcccctgctg tttcgcggcg ttgggtttat tgactgaaat gccccatgtt gtgcgctcaa
    56041 cgtcagctcc cggcgggagc ggtacttcca cctgaccata gcgcagaact gcatcctgct
    56101 cgctggtaag cgtaatttca tcgagataca gcgcatcgct gtcaatctca ttgctggtca
    56161 ccggctgcag agtaaagctc atctcttctt caccggggat aagccgatac aactggcgtt
    56221 caacggtcac cggaatgtta ctttgttgag ccgtttttgc cggttcacgc cagcggacct
    56281 gcacatttgc ggcgataatt cgtcaccaaa agagagaatg tccggcacac cctaaccaac
    56341 ccaacgccag tcttcgcccc ctccagttaa cttatgttta gcccatgcgc ccgcaggcgc
    56401 aggcaaaaca actggaggca ttgtcgccat atatttcgcc agccagttca tggccagcgc
    56461 gcgctcaata gtggattgct cagcggtcaa accattaaaa tagcggccgt ctgggtagcg
    56521 tcaccaccgc tattgagcag cacaaccgtg tgcgccagcg gctgattgct gtttgccgct
    56581 ttatcccacg catattgctg aacctgtggc atcaccgtgg acttcaactg cgcttttttc
    56641 agcaacgtca tggttaagac gtttgccacc gcatctgcca gtggagattc cggtgtatcg
    56701 aggatcaggc tgtcattgat atcgcggatg tcttcctcag agaaatcttc atctttagtt
    56761 ccgcgccggg cgatagcttc atccccccct ttcaacaacg ttttgcacgg cagattcatc
    56821 tcctgtgccc acgccagcac cagcgcccga tgcaataacg gcatattatc cgcctgctcg
    56881 gcataactgt cgagcatatg ctgccagtat tccggttgtt gcgttacgcc gagcgcctgg
    56941 ctggcctgcc agtcggcgta ccatgcccat gccgtaagga aggcgtcacc attgccatct
    57001 tcaccccacc aggtaaagcg cgctccgggc cccgccagtt gcatcagccg cagacggtta
    57061 tcctgaatca tctgacgaat gtcgttagcg gcggcacttt gatgatcggc aagcgaacgc
    57121 caggcgagac tgagcgggat cagacggcta ccggtgttga tcaccccacc ccacggttca
    57181 tcgactaacg catcaagatt gttgcggaaa atctcctgcg gcgtttcact actttgtagc
    57241 cggatattac tcgcctgctc gggcaacatc agcgcgttct cgccaccgcc gagcatgaca
    57301 tttttctgct gttcaacggg ccagctgttg tccacaaaag acagttttgt gctaatgctg
    57361 tcctgcactt gcccattttg ttgcagttct gcacttaaca agccagattg ctgaatattc
    57421 tgcgtcagcg aaatataatt cgcccctttg tgcagcgtca gcgtctggcg catctcagcg
    57481 cctgcaaatt tagtcaccag cgccaccggt tcgttatcct gctgactgaa gataaacagt
    57541 cctgccgccg gtttgtcgcc cacgcgatac accgttggca tactccactt catgtagaga
    57601 tttttttccg aacgcagata agcacgcccc tgcccgacca gcccgtcgcc gttcatccca
    57661 cgcgcggtga tacgccagcg ggttaacgaa tcaggcatca ggaacgtgaa atacgctttg
    57721 ccttgtttat cggttgtgag tgacggcatc catgccgcgg tatccacctc ttcacgccgt
    57781 ggacgttcaa gcatttttac tcgccgctcg ctgcggttag tcgcgccagg cgcaaccggc
    57841 tcgctggaga gcgcctggtc atagctgata aacgacagac tggagctggt acgcacattg
    57901 ttacgcccca gcggatagaa aaatttgccg atattcggcg cgatttctgg ttgcagcgcg
    57961 tagatcattt catcgaccac gcctaccgtt agctgcgcag aaacaggttt acctttcagc
    58021 gacgaggtta
//
LOCUS       DAJTSF010000013        55418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000013 DAJTSF010000000
VERSION     DAJTSF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.013"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO335_000869"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO335_000869"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6001359.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO335_000870"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO335_000870"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6001360.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO335_000871"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO335_000871"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6001361.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="OO335_000872"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="OO335_000872"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6001362.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="OO335_000873"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="OO335_000873"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6001363.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="OO335_000874"
     CDS             7826..9559
                     /locus_tag="OO335_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6001364.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="OO335_000875"
     CDS             9708..10559
                     /locus_tag="OO335_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6001365.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="OO335_000876"
     CDS             complement(10546..10887)
                     /locus_tag="OO335_000876"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6001366.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="OO335_000877"
     CDS             complement(10889..11767)
                     /locus_tag="OO335_000877"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6001367.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="OO335_000878"
     CDS             complement(11733..14030)
                     /locus_tag="OO335_000878"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6001368.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="OO335_000879"
     CDS             complement(14081..14401)
                     /locus_tag="OO335_000879"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6001369.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="OO335_000880"
     CDS             complement(14416..15495)
                     /locus_tag="OO335_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6001370.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="OO335_000881"
     CDS             15804..16907
                     /locus_tag="OO335_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR6001371.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="OO335_000882"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="OO335_000882"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6001372.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="OO335_000883"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="OO335_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="OO335_000884"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="OO335_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="OO335_000885"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="OO335_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO335_000886"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO335_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6001373.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO335_000887"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO335_000887"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6001374.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO335_000888"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO335_000888"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6001375.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO335_000889"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO335_000889"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6001376.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO335_000890"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO335_000890"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6001377.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="OO335_000891"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="OO335_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6001378.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="OO335_000892"
     CDS             30120..30728
                     /locus_tag="OO335_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6001379.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO335_000893"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO335_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6001380.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="OO335_000894"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="OO335_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6001381.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="OO335_000895"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="OO335_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6001382.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="OO335_000896"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="OO335_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6001383.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHNKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="OO335_000897"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="OO335_000897"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6001384.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="OO335_000898"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="OO335_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6001385.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="OO335_000899"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="OO335_000899"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6001386.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="OO335_000900"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="OO335_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6001387.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO335_000901"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO335_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6001388.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="OO335_000902"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="OO335_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="OO335_000903"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="OO335_000903"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6001389.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="OO335_000904"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="OO335_000904"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6001390.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="OO335_000905"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="OO335_000905"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6001391.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO335_000906"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO335_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6001392.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="OO335_000907"
     CDS             complement(44843..45142)
                     /locus_tag="OO335_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6001393.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="OO335_000908"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="OO335_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="OO335_000909"
     CDS             45895..46494
                     /locus_tag="OO335_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6001394.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="OO335_000910"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="OO335_000910"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6001395.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO335_000911"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO335_000911"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6001396.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO335_000912"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO335_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6001397.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO335_000913"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO335_000913"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6001398.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO335_000914"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO335_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6001399.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO335_000915"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO335_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6001400.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="OO335_000916"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="OO335_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6001401.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="OO335_000917"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="OO335_000917"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6001402.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO335_000918"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO335_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6001403.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13494 a        15016 c        14164 g        12744 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatatggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc ataacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttat tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtggg
//
LOCUS       DAJTSF010000014        53633 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000014 DAJTSF010000000
VERSION     DAJTSF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.014"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(2..298)
                     /locus_tag="OO335_000919"
     CDS             complement(2..298)
                     /locus_tag="OO335_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR6001404.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            513..1241
                     /gene="tonB"
                     /locus_tag="OO335_000920"
     CDS             513..1241
                     /gene="tonB"
                     /locus_tag="OO335_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6001405.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="OO335_000921"
     CDS             complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="OO335_000921"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6001406.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEVAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1784..2323)
                     /locus_tag="OO335_000922"
     CDS             complement(1784..2323)
                     /locus_tag="OO335_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6001407.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2353..3096)
                     /locus_tag="OO335_000923"
     CDS             complement(2353..3096)
                     /locus_tag="OO335_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6001408.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3124..3213)
                     /locus_tag="OO335_000924"
                     /pseudo
     CDS             complement(3124..3213)
                     /locus_tag="OO335_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3453..4091
                     /gene="ompW"
                     /locus_tag="OO335_000925"
     CDS             3453..4091
                     /gene="ompW"
                     /locus_tag="OO335_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6001409.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4151..4657)
                     /locus_tag="OO335_000926"
     CDS             complement(4151..4657)
                     /locus_tag="OO335_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6001410.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4703..5203)
                     /locus_tag="OO335_000927"
     CDS             complement(4703..5203)
                     /locus_tag="OO335_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6001411.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5289..5468)
                     /locus_tag="OO335_000928"
     CDS             complement(5289..5468)
                     /locus_tag="OO335_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR6001412.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="OO335_000929"
     CDS             complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="OO335_000929"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6001413.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="OO335_000930"
     CDS             complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="OO335_000930"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6001414.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(7859..9220)
                     /gene="trpCF"
                     /locus_tag="OO335_000931"
     CDS             complement(7859..9220)
                     /gene="trpCF"
                     /locus_tag="OO335_000931"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6001415.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(9221..10816)
                     /gene="trpD"
                     /locus_tag="OO335_000932"
     CDS             complement(9221..10816)
                     /gene="trpD"
                     /locus_tag="OO335_000932"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6001416.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(10816..12378)
                     /gene="trpE"
                     /locus_tag="OO335_000933"
     CDS             complement(10816..12378)
                     /gene="trpE"
                     /locus_tag="OO335_000933"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6001417.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(12400..12493)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(12470..12514)
                     /gene="trpL"
                     /locus_tag="OO335_000934"
     CDS             complement(12470..12514)
                     /gene="trpL"
                     /locus_tag="OO335_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR6001418.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            12652..13533
                     /gene="yciV"
                     /locus_tag="OO335_000935"
     CDS             12652..13533
                     /gene="yciV"
                     /locus_tag="OO335_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6001419.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            13530..14150
                     /locus_tag="OO335_000936"
     CDS             13530..14150
                     /locus_tag="OO335_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6001420.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            14251..15123
                     /gene="rluB"
                     /locus_tag="OO335_000937"
     CDS             14251..15123
                     /gene="rluB"
                     /locus_tag="OO335_000937"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6001421.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(15163..15753)
                     /gene="cobO"
                     /locus_tag="OO335_000938"
     CDS             complement(15163..15753)
                     /gene="cobO"
                     /locus_tag="OO335_000938"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR6001422.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(15750..16508)
                     /locus_tag="OO335_000939"
     CDS             complement(15750..16508)
                     /locus_tag="OO335_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6001423.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            16728..17777
                     /gene="sohB"
                     /locus_tag="OO335_000940"
     CDS             16728..17777
                     /gene="sohB"
                     /locus_tag="OO335_000940"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6001424.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(17813..18064)
                     /locus_tag="OO335_000941"
     CDS             complement(17813..18064)
                     /locus_tag="OO335_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6001425.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            18444..21041
                     /gene="topA"
                     /locus_tag="OO335_000942"
     CDS             18444..21041
                     /gene="topA"
                     /locus_tag="OO335_000942"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6001426.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            21251..22225
                     /gene="cysB"
                     /locus_tag="OO335_000943"
     CDS             21251..22225
                     /gene="cysB"
                     /locus_tag="OO335_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6001427.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            22520..22684
                     /locus_tag="OO335_000944"
     CDS             22520..22684
                     /locus_tag="OO335_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6001428.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            22687..22854
                     /locus_tag="OO335_000945"
     CDS             22687..22854
                     /locus_tag="OO335_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001429.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            22968..23063
                     /locus_tag="OO335_000946"
     CDS             22968..23063
                     /locus_tag="OO335_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001430.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            23227..25902
                     /gene="acnA"
                     /locus_tag="OO335_000947"
     CDS             23227..25902
                     /gene="acnA"
                     /locus_tag="OO335_000947"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR6001431.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(25966..26556)
                     /gene="ribA"
                     /locus_tag="OO335_000948"
     CDS             complement(25966..26556)
                     /gene="ribA"
                     /locus_tag="OO335_000948"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6001432.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            26726..27490
                     /gene="pgpB"
                     /locus_tag="OO335_000949"
     CDS             26726..27490
                     /gene="pgpB"
                     /locus_tag="OO335_000949"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6001433.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            27639..27947
                     /gene="lapA"
                     /locus_tag="OO335_000950"
     CDS             27639..27947
                     /gene="lapA"
                     /locus_tag="OO335_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6001434.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            27954..29123
                     /gene="lapB"
                     /locus_tag="OO335_000951"
     CDS             27954..29123
                     /gene="lapB"
                     /locus_tag="OO335_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6001435.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            29316..30053
                     /gene="pyrF"
                     /locus_tag="OO335_000952"
     CDS             29316..30053
                     /gene="pyrF"
                     /locus_tag="OO335_000952"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6001436.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            30053..30379
                     /gene="yciH"
                     /locus_tag="OO335_000953"
     CDS             30053..30379
                     /gene="yciH"
                     /locus_tag="OO335_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6001437.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(30505..30723)
                     /gene="osmB"
                     /locus_tag="OO335_000954"
     CDS             complement(30505..30723)
                     /gene="osmB"
                     /locus_tag="OO335_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6001438.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(30992..31741)
                     /locus_tag="OO335_000955"
     CDS             complement(30992..31741)
                     /locus_tag="OO335_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6001439.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(31831..32004)
                     /locus_tag="OO335_000956"
     CDS             complement(31831..32004)
                     /locus_tag="OO335_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001440.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            complement(32152..34126)
                     /gene="pdeR"
                     /locus_tag="OO335_000957"
                     /pseudo
     CDS             complement(32152..34126)
                     /gene="pdeR"
                     /locus_tag="OO335_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            34180..34283
                     /locus_tag="OO335_000958"
                     /pseudo
     CDS             34180..34283
                     /locus_tag="OO335_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(34361..36295)
                     /gene="rnb"
                     /locus_tag="OO335_000959"
     CDS             complement(34361..36295)
                     /gene="rnb"
                     /locus_tag="OO335_000959"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6001441.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(36363..37490)
                     /locus_tag="OO335_000960"
     CDS             complement(36363..37490)
                     /locus_tag="OO335_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR6001442.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(37634..38422)
                     /gene="fabI"
                     /locus_tag="OO335_000961"
     CDS             complement(37634..38422)
                     /gene="fabI"
                     /locus_tag="OO335_000961"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6001443.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            complement(38790..39143)
                     /locus_tag="OO335_000962"
     CDS             complement(38790..39143)
                     /locus_tag="OO335_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6001444.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            complement(39211..40017)
                     /gene="sapF"
                     /locus_tag="OO335_000963"
     CDS             complement(39211..40017)
                     /gene="sapF"
                     /locus_tag="OO335_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6001445.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(40019..41011)
                     /gene="sapD"
                     /locus_tag="OO335_000964"
     CDS             complement(40019..41011)
                     /gene="sapD"
                     /locus_tag="OO335_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6001446.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(41011..41901)
                     /gene="sapC"
                     /locus_tag="OO335_000965"
     CDS             complement(41011..41901)
                     /gene="sapC"
                     /locus_tag="OO335_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6001447.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(41888..42853)
                     /gene="sapB"
                     /locus_tag="OO335_000966"
     CDS             complement(41888..42853)
                     /gene="sapB"
                     /locus_tag="OO335_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6001448.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(42850..44493)
                     /gene="sapA"
                     /locus_tag="OO335_000967"
     CDS             complement(42850..44493)
                     /gene="sapA"
                     /locus_tag="OO335_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6001449.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(44806..45051)
                     /locus_tag="OO335_000968"
     CDS             complement(44806..45051)
                     /locus_tag="OO335_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6001450.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(45185..46570)
                     /gene="puuP"
                     /locus_tag="OO335_000969"
     CDS             complement(45185..46570)
                     /gene="puuP"
                     /locus_tag="OO335_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6001451.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(46560..46724)
                     /locus_tag="OO335_000970"
     CDS             complement(46560..46724)
                     /locus_tag="OO335_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001452.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(46873..48291)
                     /gene="puuA"
                     /locus_tag="OO335_000971"
     CDS             complement(46873..48291)
                     /gene="puuA"
                     /locus_tag="OO335_000971"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6001453.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            48503..49267
                     /gene="puuD"
                     /locus_tag="OO335_000972"
     CDS             48503..49267
                     /gene="puuD"
                     /locus_tag="OO335_000972"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR6001454.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            49294..49851
                     /gene="puuR"
                     /locus_tag="OO335_000973"
     CDS             49294..49851
                     /gene="puuR"
                     /locus_tag="OO335_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6001455.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            50001..51488
                     /gene="puuC"
                     /locus_tag="OO335_000974"
     CDS             50001..51488
                     /gene="puuC"
                     /locus_tag="OO335_000974"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR6001456.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            51490..52770
                     /gene="puuB"
                     /locus_tag="OO335_000975"
     CDS             51490..52770
                     /gene="puuB"
                     /locus_tag="OO335_000975"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR6001457.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            52808..>53633
                     /locus_tag="OO335_000976"
     CDS             52808..>53633
                     /locus_tag="OO335_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6001458.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPL"
BASE COUNT        13442 a        13464 c        13883 g        12844 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tagtttttct actgaaatga ttatgacttc aataaccctt gatttacctc
      541 gccgcttccc ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcggggc
      601 tgctctatac ctcggtacat caggttattg aactacctgc gcctgcgcaa ccaatttctg
      661 tcacgatggt tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc
      721 cggtggtaga gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg
      781 tggtcattga aaagccgaag ccgaaaccta agccaaaacc gaagccggtg aaaaaggtac
      841 aggagcagcc aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa
      901 atacggcacc cgcgcgcccc acatcaagta cggcaacggc tgcaaccagc aagccggtta
      961 ccagtgtggc ttcaggacca cgcgcattaa gccgtaatca gccgcagtat ccggcacgag
     1021 cacaggcatt gcgcattgaa gggcaggtta aagttaaatt tgacgtcacg ccggatggtc
     1081 gcgtggataa cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga
     1141 aaaatgcgat gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga
     1201 atatcctgtt taaaattaac ggcaccaccg aaattcagta agcagaaagt caaaagcctc
     1261 cgaccggagg cttttgacta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca
     1321 accgctacat acttaaataa tgcttctgtc gctttatagc gttgcccgat tggttcagac
     1381 gctacttttt tcacccacac ttcaatatta atgctgaccg atgtcgtccc tttctggaca
     1441 cagcgtgcat agcagcacac cacatcgccg accgcaaccg gccgtaagaa agtcattcct
     1501 tcaacccgca cagtcactac gcgaccgtgg gcaacttctt ttgccagaat agcgccgcca
     1561 atatccattt gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg
     1621 ggcatggcta aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg
     1681 gtaaaaccga cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga
     1741 acagaggaga tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct
     1801 gcggcatgtg gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg
     1861 tcaggccaaa gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga
     1921 tgtttgccag accgcaaagg ataaagaaaa cagcccaggc cagatttagc ttcgaccata
     1981 ccggttgcgg cagcgtgagt tctttactca gcatccgctg aattagcggc tttttcatca
     2041 cccattggct gactaacagg gctcccgcaa acagagcata aatgactgta accttccatt
     2101 taataaactc atcattgtgg aagaacagcg tcaagccacc gaagacgacc accagaacaa
     2161 aagtgatcag ggccatcttc tcaaccttac gaaagcgaac ccagctatag ataagcacaa
     2221 tcgccgtggc gacgatcagc gctgcagtag ccgcatagat gtcataaatc ttgtaaaacg
     2281 cgaaaaagac aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc
     2341 aaatgcttaa ggttattggc gaatcaacat atacaggcgg aacagataga tgagcaatat
     2401 ggcagaaatc aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc
     2461 ggcaaaagaa gaggcaaaaa gcagcaacag tgtttttgcc agcaaccagc tcagtactgc
     2521 gggtgccacc agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa
     2581 aatgcccatt ttgtcctgta ccagcatcac cggtgccagg gccagtaaaa tggccatgat
     2641 aattcccgga acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat
     2701 cagaataaat aacttgggca atatcggcgc actggcacca atagctcgca gcgcactgac
     2761 tctctgaccc gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt
     2821 accgattaat tctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgctgttc
     2881 cggtgacata ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc
     2941 attgagctgc gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat
     3001 aaacgcacat agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc
     3061 ggtgtcacgg tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag
     3121 gtgttaatta gcgggcaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt
     3181 atggaaaagc atatctttgt aaagcggagg taattgctgg ccttcaggcg tgggcaaagc
     3241 gcggcaagtg gcgatcgagt taatatttgc gtagagaaaa tatttaaaaa ttgatttaaa
     3301 tcacattaac caggattctc aatgcaactt ctaaattaat ccagatcaat aaatggtgaa
     3361 ttatcatatg taatgtgatc tatgtaggat catttgttac tccaatgtag gtatattcgt
     3421 cacgttttta taaccataac gacggagcgg atatgaaaaa gttaacagtg gcggctttgg
     3481 cagtaacaac tcttctttct ggcagtgcct ttgcgcatga agcaggcgaa ttttttatgc
     3541 gtgcaggttc tgcaaccgta cgtccaacag aaggtgctgg tggtacgtta ggaagtctgg
     3601 gtggattcag cgtgaccaat aacactcaac tgggcttgac gtttacttat atggcgaccg
     3661 acaacattgg tgtggaatta ctggcagcga cgccgttccg ccataaaatc ggcacccggg
     3721 cgaccggcga tattgcaacc gttcaccatc tgccgccaac actgatggcg cagtggtatt
     3781 ttggtgatgc cagcagcaaa ttccgtcctt acgttggggc cggtattaac tacaccacct
     3841 tctttgataa tggatttaac gatcatggca aagaggcggg gctttccgat ctcagtctga
     3901 aagattcctg gggagctgcc gggcaggtgg gggttgatta tctgattaac cgtgactggt
     3961 tggttaacat gtcagtgtgg tacatggata tcgataccac cgccaattat aagctaggcg
     4021 gtgcacagca acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat
     4081 atcgttttta attccgcaca aaaacgaccc cgtaatatac ggggtcaata aggacatggt
     4141 ataaagggta ttatttcttc gcttctacgc catcagtttc agaacgaatt aaaaatttct
     4201 cagttgtttg cggaatatgc tgaatcagcc agtcggccat ttgcttttcc tcatttaaaa
     4261 tcgcttcgat gatcggaatt gagactgtat caccggcatt ttttgctgct gctaatagtg
     4321 aggtgtaaca ggtgatttca aattgctcga agacatatcc gccaatagag cctttaacta
     4381 tttcatcaga agggaatata ccaccgattg actgcccaag cgcagccatt ttactcatgg
     4441 aatctttaat gactgaacgt gaaatgtcat tacgatcaag aatagtttcc agttgaacaa
     4501 tctggttttt ggtttcacta agatgttgtt caatacgagc gcgtagttca ggataattat
     4561 ctatacggct ggccatggat tcaagcatag attcggcttg cttttccatt gcgtgggcgt
     4621 cacgtaacca gtcatgataa tgttcaatac gattcatgaa aatgtcctct ttataaaatg
     4681 actaaaagtt aaaattcata tttcaggctt tattttcggc tttcttattt acgttattaa
     4741 gggccagatc agtcagtttg atgtcggtgg ccttttcttc ttccagggtt tctttcagaa
     4801 gcttcgctgc tttacggtaa cctaattgtt cagccagcgt cgctaatgtc ccgtaactgg
     4861 caatctcata atgctcgact ttctgtgctg cggcaatcag tgcggcatca cgcacttcgt
     4921 ttttctcggt actttcgatg acctcattag cttcttcaat aagaccttcc attgccacac
     4981 atttcatgcg cttaattttc agatttgatt ctgattccac aacttgatca attcgttcaa
     5041 tctgtccatg agtttcctcg aggtgcgcat gaaaagcctg acttaatttt tcatttgatg
     5101 ttgctcttgc gagttttgcc agtgcccggg ttaattgttt ttctgcgctg taggtatctg
     5161 aaagcagttg aataaataca tcttcaatgg tcttcatatt catatttttc tccagtgaaa
     5221 tcactgcgag ccatacggcc cgcagatgca attatcttta tcagctgata ttaataatta
     5281 acggtgaatc aggatttgcc tgatttatta ccaccgcttt gttgaccgcc ttttttaccc
     5341 gcttcagatg cgcgttgcgg atcattttta aaattaccgc cgctatgctg accgccttta
     5401 cggcctgcgt cggatgcctt ctcacggtct tcggcgaaat ttcctgaact accacgatgt
     5461 tcggccatgt tatttctccc gttgcgttgc attgtttcat taatatgagt gttgtgtgtc
     5521 gacactcatt aaaattagtc actaatgaga attagtcaaa ttaagcgcaa cgagaagata
     5581 gagggaaata tattttgagg aacattctgg atatattaac aattacctga ggaatatgtg
     5641 acttagagaa aattattgat tttactggtg ttatgttgcg ggattaattt gtttgattat
     5701 attactgttg ggcggaaaaa tgacgtaagt tgacgttcga ccggggtaag cgaaacggta
     5761 aaaagataaa tattaaatga atttaggatt tttccggctt cattaaagaa agttaaaatg
     5821 ccgccagcgg aactggcggc tgtgggatta actgcgcgtc gccgctttca tcggttgtac
     5881 aaaaactttc agtgccgcca gcattttctc tggctcatta atatgttgct cgatgatttt
     5941 aacaatggcc gaaccagaaa tcgcgcccgc agctcctgca tcaatcgctg cttttacctg
     6001 atccggggcg gaaataccaa acccctgcaa tggaggtgca gcgttgtact ctttcagctt
     6061 cgcaaccaga tgattgaggg gtaacgcggc gcggttttct gcgccggtca cgcccgctcg
     6121 tgacagcaaa tatgtgtaac cacgaccgta agaggctatc tggcgcagca ggtcgtcatc
     6181 ggcatttggc gggcagatga agataggtgc gacattatgg cgcaacgcgg cctggcggaa
     6241 gggcgcggac tcttcaactg gcacatcggc aaccagcacc gaatcgacgc cgactttctc
     6301 gcactgggca taaaactcat caatgccttt gttaaacacc agattggcat acatcaacag
     6361 gccaatggga atggtcgggt gtttctggcg aatcagtgcc agcatttcaa aacattgtgc
     6421 cggagtcaca cctgccgcaa aggcgcgcag agtggcgttt tgaatggtcg ggccatccgc
     6481 cagtgggtcg gagaagggga tacctaactc cagcgcgtca gcaccggctt caattagcgt
     6541 atcgataatt ttcaatgact gctcaatgcc cggatcaccg agcgtgacga aaggaacgaa
     6601 tgcgccttct ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt ccatcagatt
     6661 tcccctcgtg ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc gcgaccggaa
     6721 aggttaacca ccagtagctg ctctttatcc gggttttcgc gcatcatttt cagcgcatgg
     6781 gccagggcgt gggaggattc cagcgccggg atgatccctt cgtgcaggca cagcgttttg
     6841 aaggcttcaa gggcttcatc atcggtaata gacacgtaat cagcgcgtcc agtgctgtta
     6901 agatacgcgt gttgtggacc gacagacggg aaatccagtc cggcggagat ggagtaagat
     6961 tcttcaatct gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa ataaatgccc
     7021 acgcgaccat gttttagcgg tgcgccgtgc tcgccagttt cgataccgtg accaccaggc
     7081 tccacaccaa tcaggccgac gttggtttca ttgatgaaat cagcaaacat gccgatggca
     7141 ttcgaaccgc cgccaacaca ggcgataacg gcatccggca ggcgaccttc tctttccaga
     7201 atctgcgctt tggtttcttc gccaatcatc cgctgaaact cacggacaat ggtcggataa
     7261 ggatgcgggc cagccgcggt gcccagcata tagtgcgcgg tttcataact gccggaccag
     7321 tcgcgtagcg cctcattaca ggcatctttc agcgtggcgg aaccgctatg taccgggatc
     7381 acttccgcac ccattaagcg catacgaaaa acgttaggcg actggcgttc aacgtctttg
     7441 gcacccatat aaatacggca tttcaggccg agcagggcgc tggcaagggc cgacgccacg
     7501 ccatgctgac cggccccggt ttcggcgatg atttcggttt tacccatccg cttcgccagc
     7561 aacgcctgcc ccagcacctg gttggtttta tgtgcgccgc cgtgcagcaa atcttcacgc
     7621 ttgagataca gcgtggtgtt cgtcccggct gtaatgttct ggcatttggt cagtgcggtt
     7681 ggacgcccgg catagttttt cagcaggtcg ttgaactggg cttgaaattc aggatctttt
     7741 tgcgcgctga caaaagcttc ttccagctgg cgcagagcag gcatcaggat ttgtggcacg
     7801 tacatgccgc caaactcacc aaaatagggg ttaagtaatg ttgtcattgt tcctttcctt
     7861 aatatgcgcg cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg atgcccggtt
     7921 gcgactctac agcagaatta aaatcaagtc cggcgcagcc ggtttgtgcc gcttccacgc
     7981 agttatctgc gcctaagccc cccgccagca gaacgttgcc aagcgattga ccatttaata
     8041 gtgaccagtc aaaacgttgc ccgcttccac cctggccgtt gtctaaaaca tatttatcaa
     8101 cgtgctgaaa ctcgcgggcg ggcagggttt caccgacgct taatgctttc cagatggcaa
     8161 catgtgctgg cagagcttca cgcagcgtat cgatatacag ctgatcttca ttgccatgca
     8221 gttgcactgc cgccagcgat aacaccttag ctttgtccac cacatcggca atatcgtgat
     8281 tgcggaacac gccaacatac tgcaacggtg ccgcagccat cacttcctgc gcctgttcaa
     8341 cgttgacgca acgcggtgat gtcgcaacaa aaatcaaccc accgtaaatc gcgcccgcgt
     8401 cataagctgc tttagcatct tgcccacgcg tcaggccaca tactttattc tcacccagca
     8461 acacccggcg cacggcggcg tgcaaatcgt catgggccat caacgccgaa ccaatcagaa
     8521 aaccgttagc gaagtggctt aactcgcgca cctgagcgta agtattgatg ccggattcgc
     8581 tgattaccgt tacgttgtgc cccagtttcg gcgcaagctc gcgggtacgg ttgagatcaa
     8641 tcgacaaatc acgcagatcg cggttgttga tgccaacgac ctttgcccct aatgcaatgg
     8701 cgcgctccag ttcctcttca ttactgactt cggtcagcac ccccatctcc agactgtgag
     8761 cgacggctgc aagctggcga tattggtcgt catccagtac tgaaagcatt aataagcagg
     8821 catcggcctg gtaatagcgc gccagataga tctggtaagg gtcgataatg aagtctttac
     8881 ataaaatcgg ctgcggggcg atttggctga cgatgaggag gaaatcaaag ctcccctgaa
     8941 aatatttctc atcagtcagc accgaaattg ccgaagcgta atgtttataa atggcggcaa
     9001 tgcgtgccgg atcgaaatca tcacggatca cgccttttga cggcgacgct tttttacact
     9061 ccagaataaa cgccgtgcgt gcaccctgta gcgcatcata aaaatgtcgt gtgctcggct
     9121 gaacctcatt ctgaaaactg gccagcggtt gctgctgttt gcgggtttct acccaaatcg
     9181 ccttgtctgc gacgattttc gctaaaacgg tttgcatcat ttaccctcgt gccgccagcg
     9241 cggtaactct gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt tgcgcattgg
     9301 cttgcagatc ttcatggcca tgcaggcgca ttaacatggc gacgttcgca gcgacggctg
     9361 cttcatgggc ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca cggttttctt
     9421 caggtgttcc gcctgccagt tgctcctggt ggtagggagt caggccaaaa tcttcagcgg
     9481 tcaattgata gctcttaatt ttgccgtcat gcagctcggc aacgattgtc ggcgcgtgta
     9541 atgaaacttc atccatcccg ccgctgtgca ccaccgccgc gcgttgatac cccagcacgc
     9601 gcaaggtttc ggcaatcggc agcaccagtt ccggactata aacaccaatt aacgccagcg
     9661 gcggatgcgc cgggttaatc aatggcccca gcacattgaa cagggtgcgg gttttcagtt
     9721 gctggcgaac cggcatcgcg tggcggaatc cggtgtgata tttcggcgca aagaggaaac
     9781 atacgcctaa ctcgtccagc gcctggcgcg atttattggc gttcatatca agattaatac
     9841 cgaatgccgc cagcagatcc gacgagccgg atttactgga gacgctgcgg ttaccgtgtt
     9901 tcgccacttt cagcccacag gccgcggcga caaacgcact ggcggtagaa atattgatac
     9961 tgttgctgcc gtcaccgcca gtaccgacga tatcagcaaa cagataatcc gggcgcggga
    10021 acggcgctgc gttttccagt agcgcggttg ctgccccggc gatctcgttc gggtgctcac
    10081 cgcgaatttt catgctcacc agcgccgccg ccagttgttc cggcttcagc tcgccacgca
    10141 ccaccgctga aaacagctgg tggctttctt gttggctaag cgtctgcgcc tgatacagtt
    10201 tttccagaat cggttgcagc gtgttggctg gctctagttt ctgctgcgcc caggccagcg
    10261 tttgttccag caggcgagcg ccctgggtgg tgagaatgga ttccggatgg aactggaatc
    10321 cacaaacgcg atccgcatcg tgacgtactg ccatcaccat gccattaaaa tgggcgttga
    10381 tggttaaacc ggccggaatg ttactgccaa ccagcgagtg ataacgcgcc accggcagcg
    10441 ggtttgttaa tccggcaaac atcgcctgac cgtcatgttc aatgctcgac gctttaccgt
    10501 gaagaatttc gcccgcctga ccgacatagc ccccgtaagc ttcgacaatt gcctgatgtc
    10561 cgaggcaaat gccaataatg ggcagcttgc cacgcaagcg ggtgaggagt tccggcatac
    10621 aaccggcttc gctcggcaca ccggggccag gagaaagcat cagtaccgga ttgctcatcg
    10681 tcgccaggcg ttcaattaag gtctgcgccg gaatatggtt gcggtaaatc accacgttat
    10741 gaccattgct gcgcaactga tctgccaggt tgtacgtaaa agagtcgata ttatcgagca
    10801 gcagaatgtc agccatcaga aagtctcctg tgcatgatgc gcggtggcaa tagcgcgcag
    10861 tacagcgcgg gctttgttac gggtttcgtc ggcttccgac tgcggaacag aatcaaggac
    10921 tacaccagca cccgcttgca cggtggcgat accgttttcc accagcgccg agcggatcac
    10981 aatgcaggtg tcgagatcgc catgcgcggt gaaataacct accgcgccgc cgtagctgcc
    11041 gcggcgacga ccttccgcct cggcaattaa ctgcatagcg cgtactttcg gcgcaccgct
    11101 taacgtcccc atattcatac aggcgcgata agcgtgcagg gcgtcaagat cgtgacgcag
    11161 ttcgccgact acgcgagaga cgaggtgcat cacataggaa taacggtcaa ctttggtgag
    11221 atcggcgatg tagcggctgc caggggtgca aatgcgtgcc agatcattac gggcgagatc
    11281 aaccagcatc agatgttcag acagctcttt atggtcagta cgcatttcca gttcaatacg
    11341 gctgtcgaga tctctgtcca gtgaaccatc ggcgcgacga ccgcgtgggc gtgttccggc
    11401 aatcgggtag atctcaatct ggcggctggt ggcatcatac ttgagcgagc tttccggcga
    11461 cgcgccaaat agggtgaaat cattatcctg cataaaaaac atgtacgggc tgggattact
    11521 ctttttcagc acgtaatagg ccgccagcgg tgacgggcag ggcagagaga aacggcgaga
    11581 tggcaccacc tggaaaattt ctccagcgcg aatcgctttt tgcaacaaac gcactacgcc
    11641 accgaactct tcatcgctct gattacattc acaacgcata tgcggcacgg aaaccactgg
    11701 cagcggcggc gcggcttcgg tcagttgctg acgtagttcg ttcaggcgag cagtgagacg
    11761 ttgtttttct tcttcattcg gagcaaacag gctggcctga atacgggtgc tttttttctg
    11821 atggtcaatc accatcagcg tttcagcgag ataaaaacag aaatcagggc agttattttc
    11881 cgctgacagt tgcggtaaat cttcaaatcc cgccacaagg tcataagaga acaggccgcc
    11941 gaagaacatt gcttctcgtt cttccttcgg tacattcaac agattctgca ataaacggaa
    12001 agcgtcaaaa accgaaaggg agcataagcg ggcgtcttca tccagcagtg gactgacagg
    12061 ggggaagcgc agcacacggc agtttggtga ttgttcactt tccacacccg caggcagggc
    12121 gttatccagt agtgccagca gggcttcgcc attgccggaa agtgcctgaa ttgtgacggt
    12181 gtcacctaat gctgtaatgc gcagcgcact gtctatcagc agcaggcttt ttaaatcatc
    12241 tttgctgtcg atatctgcgg attccagcag cagcgttgcc ggacgattcc cacacaactg
    12301 gtgaaaaagc gcagtcgggt tgtcgcgata agcgccttcg caggttagca gttcgagagt
    12361 cggtttttgt gtttgcattg ttattctcta attttgttca aaaaaaagcc cgctcattag
    12421 gcgggctggg tatctgattg ctttacgcat ggtgaataca ctgcccgttt caggaagtgc
    12481 gccaccaacc tttcagtacg aaaattgctt tcattgtcga tacccttttt acgtgaactt
    12541 gcgtactagt taactggttc gatgattaat tgtcaacagc tcatttcaga atatttgcca
    12601 gacccgttat gatgtcggcg caaaaaacat tatccagaac gggagtgcgc cttgagcgac
    12661 acgaattatg cagtgattta cgacctgcac agccatacca cagcttccga tggctgcctg
    12721 acgccagaag cattggtgca ccgtgcagtc gagatgcgcg tcggcaccct ggcgatcacc
    12781 gaccatgaca ccacagctgc aatcgcgcca gcaagagagg aaatttcacg ttcgggactg
    12841 gcgctgaatc ttattcccgg cgtggaaatt tccacggtct gggaaaatca tgaaattcat
    12901 attgtcgggc tgaatattga tattactcat ccgctaatgt gtgagttcct cgcgcagcag
    12961 acagaacggc gcaatcagcg ggcgcagcaa attgccgaaa gacttgaaaa agcgcaaata
    13021 cctggggcgc tggaaggcgc acaacgactg gcgcagggcg gcgcagtgac gcgcggtcat
    13081 tttgcgcgtt tccttgtgga gtgcggcaaa gccagttcaa tggcagatgt ctttaaaaag
    13141 tatctggcgc gcgggaaaac cggatacgtt ccgccacagt ggtgtacaat agaacaagct
    13201 attgatgtca ttcatcattc tggcggtaag gcggtgctag ctcatcctgg gcggtacaat
    13261 cttagtgcta aatggctgaa aagattggtt gcgcattttg ccgaacacca cggcgacgcg
    13321 atggaagtcg cgcagtgcca gcaatcgccc aatgaacgca cccagctggc cacacttgcg
    13381 cgtcagcatc atttatgggc atcacaagga tctgattttc atcagccatg cccgtggatc
    13441 gaactgggtc gtaaactctg gctgcccgca ggcgttgaag gcgtctggca gctatgggaa
    13501 cagccgcaga acaccacaga gagggaatta tgagccagtt tttttatatt catcctgata
    13561 acccgcagca acgtctgatc aaccaggcgg tggagatcgt gcgtaaaggc ggggtgattg
    13621 tctatccaac tgattccggc tatgcgctcg gctgtaaaat tgaagataaa aacgccatgg
    13681 agcgtatttg tcgtattcgc cagctgccgg acggtcacaa ctttaccctg atgtgtcgcg
    13741 atctttctga actctcgacc tattcattcg tggataacgt tgcgtttcgt ttgatgaaaa
    13801 acaacacgcc gggcaactat accttcatcc tgaaggggac gaaggaagtg ccacgccgtc
    13861 tgttgcagga gaaacgcaaa accatcggta tgcgtgtgcc ttctaacccg attgcgcagg
    13921 cgttgcttga agcactgggc gaaccgatgc tttccacttc gctaatgctg ccaggcagcg
    13981 aatttaccga atccgatccc gaagaaatta aagaccgtct ggaaaaacag gtagatttga
    14041 ttattcacgg cggctatctc gggcagaaac caacaacggt tattgatctt accgatgata
    14101 cgccggtcgt ggtgcgtgaa ggcgtaggtg atgtgaagcc tttcttataa gtgcgtaact
    14161 ctgtatacta cgcggcctta aaatggcgct atacggcgcc attgattctc ctgttcgacg
    14221 cctgtgaagg cgacaccaaa aggaagctct atgagcgaaa aattacagaa agtgctggcc
    14281 cgcgctggcc acggctcccg ccgtgaaatc gaatctatta ttgaagccgg tcgtgtgagt
    14341 gttgatggca aaattgctaa actcggcgat cgcgttgaag ttacccctgg gctgaaaatt
    14401 cgtatcgatg gtcatctgat ttcggtacgt gagtccgctg aacaaatttg tcgcgttctg
    14461 gcctattaca aaccggaagg tgagttgtgt acccgtaacg atccggaagg gcgtccgacg
    14521 gtgtttgacc gtctgccaaa gctgcgtggc gcacgctgga ttgccgtagg tcgtctggac
    14581 gttaacacct gtggtctgct gctgttcacc accgatggtg aactggcaaa ccgtttaatg
    14641 catccaagcc gtgaagttga acgtgaatac gccgtgcgtg tatttggtca ggttgacgac
    14701 gcgaaactgc gtgatttgag tcgtggcgtg caactggaag atggtccggc agcttttaaa
    14761 accatcaagt tcagcggcgg cgaagggatc aaccagtggt acaacgtgac tctgaccgaa
    14821 ggccgtaacc gtgaagttcg tcgtctgtgg gaagcggttg gtgtgcaggt gagccgcctg
    14881 atccgtgttc gttacggtga tatcccactg ccaaaaggtc tgccacgcgg tggctggacg
    14941 gagctggacc tcgcccagac taactatctg cgcgaactgg tggagcttcc gccagaaacc
    15001 agctctaaag tcgctgtaga aaaagaccgt cgtcgcatga aggctaatca gattcgtcgt
    15061 gcggtgaaac gtcacagtca ggtgagcggc agtcgtcgtt ctggcgggcg taataacggt
    15121 taatcaaagt atgccggacg tcatatccgg catttttaca gattaataat caatccctat
    15181 ttgcgcctta acaccggcat caaacgcatg tctgatgggg cgcagctcac ttaccgtatc
    15241 cgccagttca agaatatccc gatgacagcc tcgacccgtg ataatcaccg tctgttgatg
    15301 tggacgttca tttaacgcct gcaccacttc ttccagtggc aaatagtcat acgccaccat
    15361 atacgtcagt tcatcaagca aaaccatatc cagtgaggaa tcagcaagca tccgctttgc
    15421 atgttgccag acttcgcggc aggcggcggt atcagactcg cggttttgtg tatcccaggt
    15481 aaagcccgtt gccatcacct gaaactcaac gccatgtggc tccagcagat tgcgttcgcc
    15541 attaggccag gtgcctttaa taaactgcac gacgcccact ttttttccgt gaccaactgc
    15601 gcgtgttgcc gtaccaaatg ccgcggtggt tttgcctttt ccattgccgg taaagacgat
    15661 gataataccg cgttcatcct gggcctgggc cacacgagca tctacttttt ctttcactcg
    15721 ctgctgacgc tgttggtagc gttcatcact cattgggaaa ttcctggttt acggcccggt
    15781 tgggcgtcaa aggtcatgcc ggttttacgg cggctgtcat cgcccatcag ccagaggtag
    15841 agcggcatga tatcagcggg tgttttaagt ttctgtggat cttcggtcgg gaaggcgctt
    15901 gcgcgcattg cggtgcgcgt accgcctggg ttaatgcagt tgacgcgcag gcgctgctga
    15961 tattcatcgg ccagtacctg catcatccct tcggtggcaa atttcgacgc tgcatatgca
    16021 ccccagttgg ctcgtccctg acgtccaacg cttgatgaag taaagaccag tgaaccggcg
    16081 tccgatttga gtaataaagg aagcagtgcc tgggtgagca taaaggtggc attaacgttg
    16141 atctgcatga cgtcctgcca gacctgcgga ttttgttcgc tcattgggca aacatcgccg
    16201 agcaatccgg cattatgcaa aacaccatcc agacgcggat aattaacgac aatgcgctgt
    16261 gccagttgtt ggcaattttc ggacgtgcag gtcagcaaat cgagaataaa ccactgtggc
    16321 tgacgcccag tttcttcgtt tatgtggctg gctacctgac gtaatttttc ttcattacgg
    16381 cccaacagaa tcactgtcgc accatagcgt gcatacgtca tcgcggcttc acgaccaatc
    16441 ccatcgctgg ctcccgtcac caggataatg cgatcattga gtaaatcttg ttttggctgg
    16501 taatgcatgg ctactcctca acgacgttgt ctgtcgcgct gccagtgtac tttatgactt
    16561 tggggcttta tgcctgaaac agaagggcat ttcaatcagc ctggggcgat aacaccgcaa
    16621 aatggatact ttgtcatact ttcgctgcaa taacatctct gcgagacggc ttaacatgcc
    16681 tgttgtaaac tgtgagccaa agcgttgttt aaccaaggtg gggactcgtg gaattgttgt
    16741 ctgaatatgg tttgtttttg gctaaaatcg ttaccgttgt gctagcgatt gcggcgattg
    16801 ccgccattat tgtcaatgtt gctcaacgta ataaacgcca gcgtggcgag ttacgggtca
    16861 acaatctcag cgaacagtat aaggagatga aagaagaact ggccgcggcg ctgatggact
    16921 cacatcagca aaaacagtgg cacaaagcgc agaagaaaaa gcacaagcaa gaagcgaaag
    16981 cagcaaaagc gaaagccaaa ctgggcgagg tggcaactga cagtaaacct cgcgtctggg
    17041 tgctggattt taaaggcagc atggacgccc atgaagtgaa ctcgctacgt gaagagataa
    17101 cggcggtact cgcagcattc aaaccgcagg atcaggttgt gctccgtctg gaaagccctg
    17161 gtggcatggt gcatggttac gggttggcgg cttcgcagct gcagcgtctg cgtgataaaa
    17221 acattccttt aactgttacg gtagacaaag tcgctgccag cggcggttac atgatggcct
    17281 gtgtggcgga caaaattgtt tccgcaccgt ttgctattgt gggttccatt ggggtggtgg
    17341 cgcaaatgcc caactttaac cgcttcctga aaagcaaaga tattgatatc gaactgcaca
    17401 ccgccgggca gtataagcgc acgctgacct tgctgggtga aaataccgaa gaagggcggg
    17461 agaaattccg cgaagagctg aacgaaacgc atcagttatt taaagatttt gtgaagcgta
    17521 tgcgtccgtc tctggatatt gaacaggtgg caacgggtga acactggtac ggacaacagg
    17581 cggtagagaa aggcctggtt gatgaaatca acaccagtga tgaagttatt cttagcctga
    17641 tggaaggccg tgaagtggtt aatgtacgct atatgcagcg taaacgactc attgaccgat
    17701 tcaccggcag cgcggcagag agcgccgatc gattgttgct acgctggtgg cagcggggtc
    17761 aaaagccatt gatgtaaaag acaaacgcga ggctaagacc tcgcgttttg ctttaatcaa
    17821 ccagatgata tttttctgaa agcacatggg ccaggtgttt gaacatatta aacaccgcgg
    17881 tgcttttggc tgttggcaat ccttgttcat ctaaaaagta gtcgccggta aataccagca
    17941 cgccattacg ctgcgtgacg ccggtggctt caatccctgc gagcgtatcc tcatgctcac
    18001 gaatgatttt gttggcctct ttcaacagcg tttcgcggtc gatgggttgt gtctctttgt
    18061 tcattattta ctccttaaac aaggacatta gtctacgcca ggcatggctt gcagacaaat
    18121 ataccacgct ggtggcaaga gcgccttact ggcaactttg gattttgcat gctaataaag
    18181 ttgcgtatcg gattttatca ggtacagtgt gacgctttcg tcaatctggc aatagatttg
    18241 cttgacattc gaccaaaaat ccgtcgtgct atagcgcctg taggccaaga cctgttaact
    18301 cagtcacctg aattttcgtg aacagagtca cgacaagggg ttgatatccg cagagagcga
    18361 gtccatatcg gtaactcgtt gccagtggaa ggtttatcaa cgtgcgacgc attcctggaa
    18421 gaatcaaatt aggtaaggtg aatatgggta aagctcttgt catcgttgag tccccggcaa
    18481 aagccaaaac gatcaacaag tatctgggtt gtgactacgt ggtgaaatcc agcgtcggtc
    18541 acatccgcga tttgccgacc agtggctcag ctgccaaaaa gagtgccgac tctacctcca
    18601 ccaagacggc taaaaagcct aaaaaggatg aacgtggcgc tctcgtcaac cgtatggggg
    18661 ttgacccgtg gcacaattgg gaggcgcact atgaagtgtt gcctggtaaa gagaaggtcg
    18721 tctctgaact gaaacaactg gctgaaaaag ccgaccacat ctatctcgca accgaccttg
    18781 accgcgaagg ggaagccatt gcatggcacc tgcgggaagt gattggtggt gatgatgcgc
    18841 gctatagccg agtggtgttt aacgaaatta ctaaaaacgc gatccgccag gcatttaaca
    18901 aaccgggtga gctgaatatt gatcgtgtta atgcccagca ggcgcgtcgc tttatggacc
    18961 gcgtggtggg gtatatggtt tcgccgctgc tgtggaaaaa gatcgctcgt ggtctgtctg
    19021 ccggtcgtgt gcagtcggtg gcggtccgcc tggtggtaga gcgtgagcgt gaaattaaag
    19081 cgttcgtacc ggaagagttc tgggaagtcg atgccagcac gactacgcca tctggtgaag
    19141 cgttggagtt gcaggtgact catcagaatg acaaaccgtt ccgtccggtc aacaaagaac
    19201 aaactcaggc tgcggtaagt ctgctggaaa aagcgcgcta cagcgtgctg gaacgtgaag
    19261 acaaaccgac aaccagtaaa cctggcgctc cttttattac ctctacgctg caacaagctg
    19321 ccagcacccg tcttggtttt ggcgtgaaaa aaaccatgat gatggcgcag cgtttgtatg
    19381 aagcaggcta tatcacttac atgcgtaccg actccactaa cctgagtcag gacgcggtaa
    19441 atatggttcg cggttatatc agcgataatt ttggtaagaa atatctgccg gaaagtccga
    19501 atcagtacgc cagcaaagaa aactcacagg aagcgcacga agcgattcgt ccttctgacg
    19561 tcaatgtgat ggcggaatcg ctgaaggata tggaagcaga tgcgcagaaa ctgtaccagt
    19621 taatctggcg tcagttcgtt gcctgccaga tgaccccagc gaaatatgac tccacgacgc
    19681 tgaccgttgg tgcgggcgat ttccgcctga aagcacgcgg tcgtattttg cgctttgatg
    19741 gctggacaaa agtgatgcct gcactgcgta aaggcgatga agatcgtatc ttaccggcag
    19801 tcgataaagg cgatgctctg acgctcgttg aactaacacc agcccagcac tttaccaagc
    19861 cgccagcccg tttcagtgaa gcatcgctgg ttaaagaact ggaaaaacgt ggtatcggtc
    19921 gtccgtctac ctatgcgtcg atcatttcga ccattcagga tcgtggctat gtgcgagtag
    19981 aaaatcgtcg tttctatgcg gaaaaaatgg gcgaaatcgt caccgatcgc cttgaagaaa
    20041 atttccgcga gttaatgaac tacgacttta ccgcgcagat ggaaaacagc ctcgaccagg
    20101 tggcaaatca cgaagcagag tggaaagctg tactggatca cttcttctcg gatttcaccc
    20161 agcagttaga taaagctgaa aaagatccgg aagagggtgg tatgcgcccg aaccagatgg
    20221 ttctgaccag cattgactgc ccgacttgtg gtcgcaaaat ggggattcgc acagcgagta
    20281 ccggggtatt ccttggctgt tctggctatg cgctgccgcc gaaagagcgt tgcaaaacca
    20341 ccattaacct ggtgccggaa aacgaagtgc tgaacgtgct ggaaggcgaa gatgctgaaa
    20401 ccaacgcgct gcgcgcaaaa cgtcgttgcc cgaaatgcgg cacggcgatg gacagctatc
    20461 tcatcgatcc gaaacgtaag ttgcatgtct gtggtaataa cccaacctgc gacggttacg
    20521 agatcgaaga gggcgaattc cgcattaaag gttatgacgg cccgatcgtt gagtgtgaaa
    20581 aatgtggctc tgaaatgcac ctgaaaatgg ggcgtttcgg taaatacatg gcctgcacca
    20641 acgaagagtg taaaaacaca cgtaagattt tacgtaacgg tgaagtggca ccaccgaaag
    20701 aagatccggt gccattacct gagctgccgt gcgaaaaatc agatgcttat ttcgtgctgc
    20761 gtgacggtgc tgccggtgtg ttcctggctg ccaacacttt cccgaaatcg cgtgaaacgc
    20821 gtgcgccact ggtggaagag ctttatcgct tccgcgaccg tctgccggaa aaactgcgtt
    20881 atctggccga tgcgccacag caggatccgg aaggtaataa gaccatggtt cgctttagcc
    20941 gtaaaaccaa acagcaatat gtctcttcgg aaaaagacgg aaaggcgact ggctggtcag
    21001 cattttatgt tgatggcaaa tgggttgaag gaaaaaaata acctttaatt ctgtcaggtt
    21061 tttataaaca aagggtcgcg aaagcggccc ttttttattg catattattt tttcttcaca
    21121 cctatacact aaggctataa atgatatagt ggttatagtt agcacctttt ttattattaa
    21181 atcgtattag tcacccgcca ggtgtgacga aaaaacgatg ttctgatggc gtctaagtgg
    21241 atggtttaac atgaaattac aacaacttcg ctatattgtt gaggtggtca atcataacct
    21301 gaatgtctca tcaacagcgg aaggacttta cacatcacaa cccgggatca gtaaacaagt
    21361 cagaatgctg gaagacgagc taggcattca aattttttcc cgaagcggca agcacctgac
    21421 gcaggtaacg ccagcaggac aagaaataat tcgtatcgct cgcgaagtcc tgtcgaaagt
    21481 cgatgccata aaatcggttg ccggtgagca cacctggccg gataaaggct cgctgtatat
    21541 cgccaccacg catacccagg cacgctacgc gttaccaaac gttatcaaag gctttattga
    21601 gcgttatccc cgcgtttctt tgcatatgca ccagggcccg ccgacacaaa ttgctgatgc
    21661 cgtctctaaa ggcaatgccg atttcgctat cgccacagaa gcgctgcatc tgtatgaaga
    21721 tttagtgatg ttaccgtgct accactggaa tcgggctatt gtagtcactc cggatcaccc
    21781 gctggcaggc aaaaaagcca ttaccattga agaactggcg caatatccgt tggtgacata
    21841 taccttcggc tttaccggac gttcagaact ggatactgcc tttaaccgcg cagggttaac
    21901 gccgcgtatc gttttcacgg caacggatgc tgacgtcatt aaaacttacg tccggttagg
    21961 gctgggggta ggggtcattg ccagcatggc ggtggatccg gtcgccgatc ccgaccttgt
    22021 gcgcgttgat gctcacgata tcttcagcca cagtacaacc aaaattggtt ttcgccgtag
    22081 tacttttttg cgcagttata tgtatgattt cattcagcgt tttgcaccgc atttaacgcg
    22141 tgatgtcgtt gatgcggctg tcgcattgcg ctccaatgaa gaaattgagg tcatgtttaa
    22201 agatataaaa ctgccggaaa aataatttct tgcgttattt tcggcacctt ttatgtagcg
    22261 aaggtgccgg aatatattct cttttgttac ttatttctat accgtgcaca atatttttat
    22321 ccctcatatt tatagggtaa attacctgta aagtagtgtc taattacccg cctcgcaaat
    22381 tccagttttc aaatatctat ttcaggtcaa aagattgaat tattcactgg agacgattcg
    22441 taaattcgct ggatttttga ctaaagtttc tttaggattt gtctcatcgt atgattaatt
    22501 acaccaatca ttcaacgtct tgctgataag tgatggcgat tatatgaggt tagcaatgcc
    22561 ttctggaaat caggaacccc gccgtgatcc tgaattaaaa cgtaaagcct ggctggcggt
    22621 ttttcttggt tctgcacttt tctgggtggt tgtcgcactg ctgatttgga aagtgtgggg
    22681 ataactatgg tcggtcagga gcaactggag tcgtcaccat tatgccagca tagtgacaat
    22741 gaaccagaaa cgaaacggga atgttccgtc gttattccag acgactggca actaacatcg
    22801 cagcagcaag cctttataga actgtttgct gaagatgatc agccgaaaca ataattatca
    22861 tcattcttat tacccatttt taatgaatta aagggctttt aatacaccgc agcaataaca
    22921 gcttgagtta tctcaacaca aaataataac cgttaagggt gtagcctatg atcaacacaa
    22981 atatgaaata ttggtcctgg atgggcgcgt tttctctgtc gatgctcttc tgggccgaac
    23041 tcctctggat cattactcac tgatccttga ccccgctgcg gcggggttgt catttgcttt
    23101 gccacaaggt ttctcctctt ttatcaattt gggttgttat caaatcgtta cgcgatgttt
    23161 gtgttatctt taatattcac cctgaagaga atcagggctt cgcaaccctg tcattaagga
    23221 ggagctatgt cgtcaaccct acgagaagcc agtaaggaca cgttgcaggc caaagataaa
    23281 acttaccact actacagcct gccgcttgct gctaaatcac tgggcgatat cacccgtcta
    23341 cccaagtcac tcaaagtttt gctcgaaaac ctgctgcgct ggcaggatgg taactcagtt
    23401 accgaagagg atatccacgc actggcggga tggctgaaaa atgcccatgc tgaccgtgaa
    23461 attgcctacc gcccggcaag ggtgctgatg caggacttta ccggcgtacc tgccgttgtt
    23521 gatctggcgg caatgcgcga agcggttaaa cgcctcggtg gcgatactgc aaaggttaac
    23581 ccgctctcac cggtcgacct ggtcattgac cactcggtga ccgtcgatcg ttttggtgat
    23641 gatgaggcat ttgaagaaaa cgtacgcctg gaaatggagc gcaaccacga acgttatgtg
    23701 ttcctgaaat ggggaaagca agcgttcagt cggtttagcg tcgtgccgcc aggcacaggc
    23761 atttgccatc aggttaacct cgaatatctc ggcaaagcag tgtggagcga attgcaggac
    23821 ggtgaatgga ttgcttatcc ggatacactc gttggtactg actcgcacac cactatgatc
    23881 aacggccttg gcgtgctggg gtggggcgtg ggtgggatcg aagcagaagc cgccatgtta
    23941 ggccagccgg tttccatgct tatcccggac gtagtgggct ttaaacttac cggaaaatta
    24001 cgtgaaggta ttaccgccac agacctggtt ctcactgtta cccaaatgct gcgcaaacat
    24061 ggcgtggtgg ggaaattcgt cgaattttat ggtgatggtc tggattcact accgttggcg
    24121 gatcgcgcca ccattgccaa tatgtcgcca gaatatggtg ccacctgtgg cttcttccca
    24181 atcgatgcag taaccctcga ttacatgcgt ttaagcgggc gcagcgaaga tcaggtcgag
    24241 ttggtcgaaa aatatgccaa agcgcagggc atgtggcgta acccgggcga tgaaccgatt
    24301 tttaccagta tgttagaact ggatatgaat gacgttgaag cgagcctggc agggccaaaa
    24361 cgcccacagg atcgcgttgc actgcccgat gtaccaaaag catttgccgc cagtaacgaa
    24421 ctgaaagtga atgccacgca taaagatcgc ctgccagtcg attatgttat gaacggacat
    24481 cagtatcagt tacctgatgg cgctgtggtc attgctgcga taacctcgtg caccaacacc
    24541 tctaacccaa gtgtgctgat ggccgcaggc ttgctggcga aaaaagccgt aactcttggc
    24601 ctcaagcggc aaccgtgggt caaagcgtcg ttggcaccgg gttcgaaagt cgtttctgat
    24661 tatctggcaa aagcgaaact gacaccgtat ctcgacgaac tggggtttaa ccttgtggga
    24721 tacggttgta ccacctgtat tggtaactct gggccgctgc ccgatcctat cgaaacggca
    24781 atcaaaaaag gcgatttaac cgtcggtgcg gtgctgtccg gcaaccgtaa ctttgaaggc
    24841 cgtatccatc cgctggttaa aactaactgg ctggcctcgc caccgctggt ggttgcctat
    24901 gcgctggcgg gaaatatgaa tatcaacctg gcttctgagc ctatcggcca tgatcgcaaa
    24961 ggcgagccgg tttatctgaa agatatctgg ccatcggcac aagaaattgc ccgtgcggta
    25021 gaacaagtct ccacagaaat gttccgcaaa gagtacgcag aagtttttga aggcacagca
    25081 gagtggaagg aaattaacgt cacacgatcc gatacctacg gttggcagga ggactcaacc
    25141 tatattcgct tatcgccttt ctttgatgaa atgcaggcaa caccagcacc agtggaagat
    25201 attcacggtg cgcggatcct cgcaatgctg ggggattcag tcaccactga ccatatctct
    25261 ccggcgggca gtattaagcc cgacagccca gcgggtcgat atctacaagg tcggggtgtt
    25321 gagcgaaaag actttaactc ctacggttcg cggcgtggta accatgaagt gatgatgcgc
    25381 ggcaccttcg ccaatattcg catccgtaat gaaatggtgc ctggcgttga aggggggatg
    25441 acgcggcatt tacctgacag cgacgtagtc tctatttatg atgctgcgat gcgctataag
    25501 caggagcaaa cgccgctggc ggtgattgcc gggaaagagt atggatcagg ctcaagccgt
    25561 gactgggcgg caaaaggacc tcgtctgctt ggaattcgtg tggtgattgc cgaatcgttt
    25621 gaacgaattc accgttcgaa tttaattggc atgggcatcc tgccgctgga atttccgcaa
    25681 ggcgtgacgc gtaaaacgtt agggctaacc ggggaagaga agattgatat tggcgatctg
    25741 caaaatctac aacctggcgc gacggttccg gtgacgctta cgcgcgcgga tggtagccag
    25801 gaagtcgtac cctgccgttg tcgtatcgac accgcgacgg agttgaccta ctaccagaac
    25861 gacggcattt tgcattatgt cattcgtaat atgttgaagt aacaagtatt tgcttgccgg
    25921 ttattttgct tccggcaagc aaatgaatta cacgatgcaa gagggttatt tgttcagcaa
    25981 atggcccatt ttctcggctt tggtatcgag ataatgttcg ttattggggt tacgacctac
    26041 aatcaatggt acgcgttcaa caatattaat ccctgcttcg gtcagaattt cgactttttt
    26101 cgggttattg gttaacaagc ggacttcatt gacgccaagg agtttgaaca tatcagcgca
    26161 aagagtgaag tcgcgctcat cagcggcgaa gcctaactgg tggttagcct ctacggtatc
    26221 gtaaccttga tcctgcagtg cgtaagcgcg gattttattc agcagaccaa tgttacgacc
    26281 ttcctgacgg tgatacagca aaataccacg gccttcctcg gcaatttgcg tcaatgccgc
    26341 ttcgagctgg aagccacaat cgcagcgcaa gctgaacagg gcgtcaccgg tcagacattc
    26401 ggaatggacg cgcgcaagta ccggggtatg cccggaaata tcgccataga ctagcgcaac
    26461 atgatcgtgt ccggttgcca gttcttcaaa tcccaccatc aggaaatcgc cccatggggt
    26521 tggcagtttg gcttctgcca cacgtttaag ctgcatgaaa ttctccagat aatgctggtt
    26581 ctgtattggc ttattttgcc ataacgagaa ggggttcacc taatcacgac gcgtcgatcg
    26641 ttcacggaat ggcacaaatc tgtcaatttt tcttggaact ggcattttca gttatgattg
    26701 tgggacttat caaaaaggag aggccatgcg ttcgattgcc cgacgtaccg cagtgggagc
    26761 tgcactattg cttgtcatgc cagtagccgt atggatttct ggctggcgtt ggcaacctgg
    26821 agaacaaagt tggctactaa aagcggcttt ttgggttact gaaactgtca cccagccctg
    26881 gggcgtcatt acacatttga ttttgttcgg ctggtttctc tggtgtctgc gttttcgcat
    26941 taaggctgcc tttgtattat ttgccattct ggcggcggca atccttgttg gacaaggcgt
    27001 taaatcctgg atcaaagaca aagtacagga accacgacct tttgttatct ggttggaaaa
    27061 aacacatcat attccggttg atgagttcta cactttaaag cgagcagaac gcggaaatct
    27121 agtaaaagaa cagttggctg aagagaaaaa tattccacaa tatttgcgtt cacactggca
    27181 gaaagagacg gggtttgcct ttccttccgg tcacacgatg tttgctgcca gttgggcact
    27241 gctggccgtt ggtttgctgt ggccgcgtcg gcgaacgtta accattgcta tcttgttggt
    27301 ctgggcaacg ggagtcatgg gaagccgcct gctgctcggg atgcattggc cacgcgatct
    27361 ggtggtagct acgttgattt cgtgggcgct ggtggcggtg gcaacgtggc ttgcgcaacg
    27421 aatttgtggg ccattaacac cacctgcgga agaaaatcgc gaaattgcac aacgagaaca
    27481 agaaagttaa cgctggttga ttttccgaat ttaggcctta aatcatcaac aatgcgcgtg
    27541 gatgccattt cgcagacggc gcgaaaatgg tactttaaag ggctattgcg gtaagttgac
    27601 cataatttat tcgctctaac cacataacgg gaagtaatgt gaaatattta ctcattttct
    27661 tactggtgtt agcgatcttc gtgatttcgg tcacgttggg tgcgcagaac gatcaacagg
    27721 tgacgtttaa ttatctgtta gcgcaagggg agtaccgtat ttccacattg ctggcggtat
    27781 tgtttgctgc ggggtttgct atcggttggt tgatttgtgg cctgttctgg ctgcgagttc
    27841 gtgtttccct ggcgcgcgct gaacgtaaaa taaagcgact ggaaaaccag ctttcacccg
    27901 cgactgacgt ggctgtagtg ccgcactcgt cagcggcgaa ggaataactt tctatgctgg
    27961 agttgttgtt tctgcttttg cctgtagccg ctgcctatgg ctggtatatg ggccgcagaa
    28021 gtgcgcaaca aaacaagcaa gatgaagcca accgcttgtc gcgtgattac gtagcggggg
    28081 ttaacttcct gcttagtaat caacaggata aagcggtaga cctgtttctc gatatgctta
    28141 aagaggatac aggcaccgtt gaagcccacc ttacgctcgg aaacctgttc cgttcgcgtg
    28201 gcgaagttga tcgcgctatt cgcatccatc agaccctaat ggaaagcgcc tcgctgacct
    28261 atgaacagcg tctgttggcg attcaacaac tggggcgtga ttacatggcc gccgggttat
    28321 acgaccgcgc ggaagacatg ttcaatcagc tgaccgatga aactgacttc cgcattggcg
    28381 cgctgcaaca gttgctacaa atctaccagg ctaccagtga gtggcagaaa gcaattgatg
    28441 ttgccgaacg cctggtgaag ctgggtaaag ataaacagcg cgtcgaaatt gcccatttct
    28501 actgtgagtt agccctgcag catatggcca gcgacgatct cgatcgtgcc atgaccttgc
    28561 taaaaaaagg ggcggcggca gataaaaaca gcgcccgcgt atccatcatg atgggacgcg
    28621 tgtttatggc gaaaggagaa tacgccaaag ccgtcgaaag tttgcaacgc gtcatatccc
    28681 aggacagaga actggtcagc gaaacgctgg aaatgttgca aacctgctat cagcagttgg
    28741 gtaaaactgc cgaatgggca gaattcctgc aacgcgcggt ggaagagaac accggtgccg
    28801 atgctgaatt gatgcttgct gatatcatcg aagcgcgcga cggtagtgag gctgcacagg
    28861 tctatattac acgccagctt cagcgtcatc cgaccatgcg tgtattccat aagctaatgg
    28921 attaccactt aaatgaagcg gaagaagggc gcgccaaaga gagtctgatg gtgctgcgtg
    28981 acatggttgg cgagaaggtg cgtagtaagc ctcgttatag ctgccagaaa tgtggtttta
    29041 ccgcatacac cctctactgg cattgtccgt cttgtcgtgc ctggtcaact attaaaccga
    29101 ttcgcggtct tgatggcctg taatttttaa aaaaatccga ctttagttac aacatactag
    29161 ttattgatgt cccattgtgc tccggcaacg acggagcaga aaaagcctgc taggggaaaa
    29221 atcgcaactg ttaatttttt attttcacgg gtagaatgct cgccgtttac ctgtttcgcg
    29281 ccactcccgg tgcccatcat caagaaggtc tggtcatgac gttaactgct tcatcttctt
    29341 cccgcgccgt tacgaattct cctgtggttg ttgcccttga ttatcataat tgtgatgacg
    29401 cgctgtcctt tgtcgacaag atcgacccac gcgattgtcg tctgaaggtc ggtaaagaga
    29461 tgtttacatt gtttgggcca cagtttgtgc gcgaacttca acagcgtggt tttgatatct
    29521 ttcttgacct gaaattccac gatatcccca acactgcagc gcacgctgtc gctgctgcag
    29581 ctgacttagg cgtgtggatg gtgaatgttc atgcctccgg tggggcgcgt atgatgaccg
    29641 cagcgcgtga ggcactggtt ccgtttggca aagatgcacc gcttttgatt gctgtgacag
    29701 tgttgaccag catggaagcc agcgacctgg ccgatcttgg cgtgacactg tcacctgcag
    29761 attatgcaga acgtctggca gcactgacgc aaaaatgtga ccttgatggt gtggtgtgtt
    29821 ctgctcagga agctgtgcgc tttaaacagg tattcggtca ggagttcaaa ctggttacac
    29881 cgggcattcg tccgcagggg agtgaagctg gtgaccagcg ccgcattatg acgccagaac
    29941 aggcgttggc ggctggtgtt gattatatgg tgattggtcg cccggtaacg caatcggtag
    30001 atccagcgca gacactgaaa gcgatcaacg cctctttaca gcggagtgca tgatgagtga
    30061 ttccaacagc cgtctggtct actcaacgga aaccgggcgt attgatgagc ccaaagcggc
    30121 cccagtacgc cctaaaggcg atggagcggt gcgtattcag cgtcagacca gtggacgtaa
    30181 aggtaagggc gtttgcctga ttaccggtat cgatctcgat gatgccgaac tgacaaaact
    30241 tgcagcggaa ctgaagaaaa aatgcggctg cggcggagca gttaaagatg gagttattga
    30301 aatccagggc gataagcgtg atttattaaa atcactactt gaagcgaaag ggatgaaggt
    30361 aaaactcgca ggcggttaac ataaaaagcc acggatatat ccgtggcttt cgaatatttt
    30421 actgtgtgta ttattcagtt atttcgtact gaacagaatc aagcgtatta taattgcgag
    30481 tcgataatcg acatttattt acgattattt accgacctgg tgaccaataa caccaccgac
    30541 agctgcacca cctaatgtac ccaacgtact gccatcggtc agtactgcac cgcctaatgc
    30601 ccctgcaccc gcgccgattg cggtgttgcg gtcccgttta gaccagttag aacaggcact
    30661 cagagacatt gccaaagtaa ttgccagaac agccgcggtc atttttttgc tcgttacaaa
    30721 cataatactc tctcctgaat ttatgattca cggaagtaag ctctctataa ctataatagc
    30781 taagaataag cctggtgaaa ttattccgtg aaatctgctc gcgcgcaggg ttatatcacg
    30841 caggtgataa ttacttcctg ttatatcgct gataataatt ttatatcttg agagtgttaa
    30901 taacaggtaa atagtcttaa ttatcaacca ggaatcatct tagagcggat gatttgccaa
    30961 actgcaaatc atccgtagag aagggaaatg gttaaatgtc aacgacgtga acggtgagtt
    31021 tactgtgctc aagatgcatc agatcgctgg cgcgtatttt cgaatcggta atcacgcgat
    31081 tgaatcgctc aacgggaccg atggagtatg aatgtacagc accaaatttc gagctgtcag
    31141 tcaggactat cgcttcgcac tctttttcca gcacggcatt gaccacatcg gtgcgcatca
    31201 tatcgcgacc ggtgaatcca gtttcaggtt gccagccatc aataccaata aatgctttgc
    31261 tgaaatgtac ctgctggatg cactggcgtg tcaaagggcc aaccatactt tcgctttttt
    31321 tctggtacac gccaccgagc aaaatgactt cacaaggcgc gtctttcagc aaatgcgcga
    31381 tgtagctgct gaccgtgatg atagtgacat ttttcttctg ctcccccaga gtccgggcga
    31441 gcagggcatt gctgctgcca ttttcgataa agatggtttc gcccggttga accagtgacg
    31501 cggcaaactc ggcgagttca cgcttcagcg tatagttgct catcatacgg gtttcgacgt
    31561 catcactatc aagcgaaact gcaaagccat gtgcacggcg gaggtaactc agtttttcga
    31621 gggtgttgag atcctggcga atggtaactt cagaaactcc agtggctttt gccagatcgg
    31681 ttacgctaac ctgaccctgg tcaatgacca tctgtagaat agtttgttgt cgggaattca
    31741 tcgctgtaat tcttataacg ttataatact aaataaaaaa atactgacgc gccgggcatc
    31801 ctcaaatagg aaaacctgaa ggcacgattg ttaaatctcc cacggtgtct tcgggatgtc
    31861 tggcgtagat tcagcaacct ggcgcaagag ttctgcttta agtatctcga gattatggat
    31921 cgcagagtgg taatcgcctg ccacaaggat atctaacaca atatcaatac gttcggcaac
    31981 gcgctgggca tcaaattcag acatcaagga catccttata gcaaaagggg gaaaatgaat
    32041 aatgcaaaaa ttgccgctaa aagagaagtg tttaacagca acggctaatt atcatccagg
    32101 aatacgatat ataatgacgg gatatagcgc taagtatata tattcatcta cttatgcgcg
    32161 cttcagatag cgtttatacc agcgttcgaa ggcgacggcg ggcatcggtt tggcaaacaa
    32221 aaatccttgc cgctcattga tcccgttctt ggttaaaaaa gcatcttcct ttgcactctc
    32281 tacaccttcg gcgatcacct gaagattcaa tgcctgggcc acagcgacga tcgcccggac
    32341 cagtgactgc gagacaggtt gtttgtgaat atctcgaaca aaaacctggt caagtttgat
    32401 ggcatcgatc ggaaagcgcg ccagttgcga aagtgaagag tagccggtac caaaatcatc
    32461 cagatgcact tgcgcaccta gttggctaaa ttgttgaata acagacagtg ccagttcatc
    32521 attctcaatc agacaactct ctgtcagttc aacatctata gggcagtatt caaaattgag
    32581 ttcctggaga acctgtttca gggcggtgaa aatggtttga tcggcgagct gacgtgcaga
    32641 aatatttacc gccactcgca ggtttatgcc tttatcccgc cactttgcca cctggcgtac
    32701 gacatcgaga atcacccaac ggcctaaagg cacaattagc cctgactctt cggcgtagga
    32761 aatgaagtcc aacggtggaa tcaacccacg ttcaggtgac tgccaacgta ctagtgcttc
    32821 cagactgcgc acttcgacac gctaggtgat tttcgataac caactgatcg ttttccagtg
    32881 ctttacgcaa gttggtatcc agccagagat attcaaatac ccgctgattc atttctggag
    32941 taaaaacgca aaattgtcct cgtccgcctt ccttcgctgt gtacattgct gtgtcggcgt
    33001 gacgaataat agtcgcgctg tccgaaccat gttcgggaga gagcgaaata cctactgaac
    33061 agctggtata aacttcaatt aaaccaatac gaaaggggag gcgtaagcgg gtcaaaattc
    33121 gtgatgccat tgcttccagc gcgctttgtg aggtattgga tgccagtacc agaaactcat
    33181 cgccacctgg acgcgccaac acctggtcat gttcgagaca gcttaaaata gccaatgaca
    33241 cgtcgcgtaa taactggtca ccaaacaaat gcccataggc gtcgttgacc tttttaaaat
    33301 tatccaaatc aagatacaca accccaactt tattgttatc tgcctgatta atagcgtgat
    33361 cgattaactc ctgcattgcg ttacgattcg gcagtccggt gatactgtcg gtatttgcca
    33421 gaatacgcag tcgctcctga gtgcggcgct cttcggtaat gtcggtaccg gaacagatta
    33481 aaaaaatctc gtttttgcca ctgccgctgt ggacaaattt attgcgaaac agaaacagcc
    33541 gctggccttt acgtgttggt atccacagtt cgacttcata tgcattgccg cttcgaaaaa
    33601 atacacggtt attgcgcctg gatgccgcag cttcacgacg gctcataaac agtttaaaca
    33661 cgctttaccc aatgacgtcg tgttctttca accctgtgta atcttcacat aaccgattga
    33721 agcgttggat attcccccgg ctgtcgagaa tcactattgc ggaatgtgct tcagaaacta
    33781 cctgctcagc aaaggaaagc ccgtgtgata aatcacgagc aaccgccggt gtgtcatgcc
    33841 atgcagacgc gttgccagcc cattcccgtt tattgatttt tcgtcctacc agatgcacag
    33901 aaagatcgct ttcatcgacg gtcagactca tcatcaggct ggaggtgatg accgtcattt
    33961 ccctgatgcg agcagcctgt tcggcagata actgcaatgt acgatcaggt tctgtggttt
    34021 tggtggtaga aaagcgcaaa gcatcgctgc tttccgtcaa ccgccagtat ggattgtgcg
    34081 atccgagaaa gttgtacaac gttgtggact ccctaacggt tttcatgatg catatcctcc
    34141 ggttaacagc ggagtccgcg gttaaataaa aggaacaaca tgacttacat gaaattaacg
    34201 gcggctaaac gcattgtttt tttatatatt tatttgtaat ccagttttgg aaaaacgcca
    34261 gttttcaaac gaaagtcagt taaaaaatct gcctggatat aacgaaggta gagcggggaa
    34321 ataaacggcc catccatgag gaatgggccg tgaaaggaga ttacgcgacc gggcgcgcaa
    34381 taatgctgcg ggtttccatg cggacttcgg caatggtgac gtcaataacg tcagttactt
    34441 tgtaaaccgt ttcaccttta atttgtacag tgccgttttc ctggctgcaa accagttcat
    34501 cgcgcacagc gtgtaagaaa ggtgccggaa taaaggcgat agcgccgtta tcaaccaaac
    34561 gcacacgcat gccgccacgg ctgatatcga caatttccgc cgcgaaacgg gtgccggtcc
    34621 cggctttatc tttcaggaag cgtgcgtata accagtcgcc aacatcacgt tctgccatcc
    34681 ggttgagacg gcgacgttcg gccatttgga cagtgatttc atcctgcgga cgcgtcgcag
    34741 tttcgccttt gataaccgct ttcagcagac ggtggttgat catgtcgcca tatttacgga
    34801 tcggcgaagt ccaggtggcg tatgcttcca gaccgaggcc aaagtgagga ccgggttcag
    34861 agctaatttc agcaaatgac tggaagcgac gaatgcggct gtcgaggaaa ccagttggtt
    34921 gcgcatccag ttcacgacgc agtttgcaga aaccgtccag cgtgagcact tcttcggcat
    34981 cgacatgcag accgtgcgtt ttcagcaacg ctgccagcgc gtcggcattc gccggatcaa
    35041 agcccatatg cacgttatag atgccaaaac cgagcttatc gcgcagtacg cgggccgcac
    35101 aaatgttagc ggcaatcatc gcttcttcga cgatacggtt ggcaatgcga cgaggctcgg
    35161 cgacgatatc cagcacttca cctttttcac cgagaataaa gcggtaatcc gggcgatctt
    35221 taaacaccag tgcgtggtta tgacgccact cgccgcggcg ttggcaaatt tgcgctagca
    35281 aacggacttg ttcggcaatt gcttcacttt caggcttcca gtcaccggta ttttccagcc
    35341 agtcagaaac ctggtcatac accagcttcg ctttggattc gatggtggcg gcaaagaatt
    35401 cgatattatc ttcaatggtg ccatcagcgg agagcgtcat gcggcatgcc agtaccgggc
    35461 ggacttcatt ggcgcgcagt gagcagagat cgtcagaaag ctcgcgaggc agcataggga
    35521 tgttgaagcc aggcagatag ttggtgaatg cgcgaatttt cgcggctttg tccagcttgc
    35581 tgccttcagc aatccacgcg gttggatcgg caatcgccac aatcagctga agtttgtcat
    35641 ccggcagcgc cttagcgaaa agggcgtcat ccatatcttc agtgctggca ctgtcgatgg
    35701 tgacaaaatc cagcgaggtc agatcttcac gaaccagacc ttcatcgagc atttcggtag
    35761 cgacgccgtc tggtgcttct ttttccagat tatggcgcgc aagggtaacc caccacggta
    35821 caaagtggtc gtcaccaaaa gtgatgtatt gtgtcagttc agcatagaaa gaacgatcgc
    35881 ctttcagcgg atgacggcgc atttcggcaa ccgcccagtc gccttcttta aactcgtggt
    35941 tcaggccacg ggctgcgcgg caaggaatgg cgtcttttaa aagtggatga tcaggaacga
    36001 tggtcagacg gtcatttttg ccctgaactt tacccacgaa acgagtcagg aacggttcaa
    36061 ccagttcttc tggctctgcg gattcacgtt ctttttcact gtggatcacc gcgataattc
    36121 ggtcgccatg catgactttt ttcatctgcg gcggcggaat gaaataactt ttttgtgcgt
    36181 cgacttccag gaagccaaag cctttttctg tggcttttac caccccttca gcgcgtggcg
    36241 tctgggaatg cagttgctgt ttaagctgcg ctagcagcgg gttgtcctga aacataattg
    36301 tctattttgg tggccattag agcggctgac agttttacgc gaatctgtct gacgcggcaa
    36361 ggttaatatg tctcacccaa cgcgattttt aagcgattta tccagccaca cagcccgctc
    36421 cataccagca gattaataat ctgcgttgat gatttttcgt gttcgagtaa agggctgaac
    36481 tgggcggcgc taaagcgatc gggtgctcgg gtcagccatt gtatcgcctg aacggtagcg
    36541 cgctcaacag aatgacggtc agcccatcgg ttaatttcat tttcgccctt gcgcagaagg
    36601 gtgctgattt ttttatattc atttgatgaa tccatacagg tggaaaaaca agcaggactg
    36661 ccatttatac gcgcgcataa caacgccgca agctgattat ccggctgtgc attgacccgt
    36721 gtgctgttta acaacttttc gagaagggaa agtgaaatag ggtggagaga gagtgtttcg
    36781 gccagttggc aaagttctgc agttgaactt tttgtgtgct cttcaggtag tttttccacc
    36841 tcatagctgc ttcgccagcg tattgattca tcagcaaaca gtgacgcgtc ggcgtagttt
    36901 tgtatcgcca gcccgggtaa ccagcgtacc ggatgcccga gataagcctg aaatgtcgca
    36961 attgtccgcg cctgaaagcc aataaaacca atgatttggt taatgatcac acagtcccag
    37021 acagtaagac cgacgtcatt cagatgctgg cgcgatcggt tgtcgataat agaaggcgag
    37081 ctagccagtt ggcgggcata ttgcgtgatt tgtgccaacc gatgattact ttccctggag
    37141 gaatcaggtc cggggagcgg cgtaagtcgc gccgaatagt agttacataa ccgctggacg
    37201 ccgcaaacct gagcaaccgt cagggccgtc gacaagcgtt cataagcggt gaaggtttgt
    37261 gaacgggtca cggtaagctg gtcagggaac aactccactg caagaacgcg cgcagggacc
    37321 agccatgaaa ggaagggggc cagcgtctct tcatcaagga tcacgtcgag cagaaaggga
    37381 tcgctgacct ttgcggcttc cgggaccaga ggcagaacgt catactccgt agtactggat
    37441 tgcgtttcat gataccagtg gcttttgccg gtgatgtggc gttgttccat gggcgctcct
    37501 tggtcgtaaa ggaaatcgtt atcctgacgc aaggcgggaa ggggagaaag acggatcggc
    37561 gataacaaat atcagaaagg tataacagag ataacgggcg gcagaacgcc gcccatcttt
    37621 accaacagaa cgattatttc agttcgagtt cgttcattgc agcgatgctg aaaccgccgt
    37681 caacgtggac tacttcaccg gagataccgg cagagagatc ggagcacagg aatgccgcag
    37741 agttacccac atcttcaata gtaactgtac ggcgaatcgg ggtaacggct tcgcaatgag
    37801 ccagcatttt gcggaagtct ttgataccgg aggccgccag agtacggatc ggaccagcag
    37861 agatggcgtt aacacgcaca ccttccggac ccatcgcgtt cgccatatag cgcacgttcg
    37921 cttccagaga cgcttttgcc agacccataa cgttgtagtt cgggatagcg cgctcagcgc
    37981 caaggtagga aagggtcagc agggcagaac ccggattcag catggagcgg caagcttttg
    38041 ccattgcaac gaagctgtag gagctgatgt cgtgggcaat tttgaagcct tcacgggtaa
    38101 cggcgttaac atagtcacca tccagctgat cgccaggtgc aaaaccaata gagtgtacga
    38161 aaccgtcaaa tttcggccaa actttcccca gttcagcgaa catggtgtcg atgctggcat
    38221 cttctgcaac atcgcactgc agaacgatgt cagaacccaa ttgagcggca aattcttcta
    38281 cgcggccttt cagtttgtcg ttctggtagg tgaatgccag ttcagctcct tcgcggtgca
    38341 tcgcctgagc gataccgtag gcgatggaca gtttgctggc aacaccggtt accagaatgc
    38401 gcttaccgga aagaaaaccc atagctttaa tccttattgt tgatgcttgt tgtgcctgaa
    38461 aatcaggcga ttcgtcgttt tagtaaacag tacgaacaga taaacggtta ttataatcaa
    38521 cctggctgtg agtagctata gttaccaggt ccgaccggag caggctgcgg cagggggggc
    38581 gcttttcccc ttaccctaac cctctcccca gaggggcgag gggaccatat tgtgcaaata
    38641 ttgttacccc agcaacaaac aggctcatac agcccctaac ccttttatgg cgatggctgg
    38701 gatggttcag accttgccga atattctcca gcactgtctc catgttttca ccacaacagc
    38761 tattcggctc ggtcggcccc ctcgcccttt cagggtgagg gtgaccggcg gttcagttcc
    38821 tgcagaataa tctccagcac cgcctcctca ttacaatcaa tctcgttatt ccagaaacgc
    38881 agcacggtcc agccctgcga ctcaagccag ctagtgcgcc tggtatcata ggcaactgcc
    38941 aaatcatgct gcccaccatc cagctcaacg actacgcgcg ccgagcagca agcaaaatcg
    39001 agaatgtagc tccccaccgg atgttgacgg cgaaatttga aatcaccaaa acgtcggctg
    39061 cgaagatatc gccagagctt tcgttcctgc aaagtgagat tgcgtcttaa atcacgcgca
    39121 tttgatttta ttttatccat cacctcattc tgacaagatt taatctcttg tcagaatgag
    39181 gtgaataatc tggaaggagg attcagaaaa ttagcgatct ttacgccacg catccgctgt
    39241 caatgcttcg ccaaagtgcc cggcaatcag ccgtttggtg agttcatgca gcggcgatgc
    39301 cagcacatcc gcggtgctgc ctcgctcgac aacctcgccc tgatgcatca ccagcacctg
    39361 gtcgctaatg tgcttcatca ttccgatatg ctgggtaaca taaatatacg aaatgccctg
    39421 tttttcctgt aactccagca tcagattaat caactgcgaa cgcattgaca tatccagtga
    39481 agcgagggct tcatcggcaa taatgacttt tgggcgcaat atcagcgcgc gcgccagacc
    39541 cagacgctgt ttttgtccgg gtgccaacat atgcggatag taactgacgt gatccggtag
    39601 cagcccaacc atacgcatcg tttcaataat ctgtttgcga cgctgttccg gttccaggtc
    39661 ggtgttcagg cgcagtggaa aatccagaat ttgtgagata cgttgacggg gattcaacga
    39721 ggtcgaagga tcctgaaaaa tcatgcgaat acgctgacta cggaaggaat aatcgccaaa
    39781 atgcagcgga tgatcgtcaa tcaataactc gccgctggta ggttctatca ttcccgccag
    39841 catttttgcc agtgtggatt tacccgaacc attctcgcca ataatcgcca gtgtctggcc
    39901 ttcacgtagc gtaaagctca agggttttac cgcttctacg gtctgacgac gaaaccaacc
    39961 ggtccggtag cggaacgttt tacttaaatt acgcacttca agcagcgttt cgatcatctc
    40021 actctttctc catgttcagc gggaaatgac aggcatagag atgatttttc gcccccgtca
    40081 aacgtggcgt cacaatgcat tctcgttgtg catacgggca acgtggcccc agacgacaac
    40141 caatcggtaa ctgttccagc aatgggatag cgccgggtag agtattgagg cgacttttat
    40201 gcggcatcgc gctgccgaag tctggtatcg cgcggatcag cgcctgggta taaggatgat
    40261 gtggcatcgt caccaactcc ttactcggcg cggtttccac tgtttgaccg cagtaaagca
    40321 cgttaatttt atccgcccat tggctaagca tttgtaagtc atggctgata agcaaaatag
    40381 tggtattgct gttttggttg agacgcgtca gcaggcgaaa gatttgcgcc tgggttgttg
    40441 gctccattga gtttgtcggt tcgtcagcaa tcagcagacg cggttgattc gccagtgcaa
    40501 tggctatcat cactttctga cattcacctt cggtcaactc atagggaaaa ctgcgcatcg
    40561 catctttgtg atctttaatc cccacgcggt gcagcagttc aatggcacgg cgtttgcgcc
    40621 agccaaaacg ctgccaccaa cggcctttat aggtccaggc tgggatgttt tgcatcaact
    40681 ggcgacccac acgttctgaa gggtcaagac acgactgcgg ttcctggaaa atcatcgaca
    40741 cgttatggcc aaccagtttg cgccgttcgc gtgcggagag acgcagcaaa tcgatatcat
    40801 caaaacgcat acggtcagca gtaacacgcc agttatcttt attcacccca caaatcgctt
    40861 tcgcaatcaa actcttgccg gaaccggatt caccaacaag accgcggatt tcaccttcgg
    40921 ttaaggtcat acttacccgg tcgacggctt taacccactc atcaccggtt ttaaattcaa
    40981 tggtcaggtt acgaatatcg agtaatggca ttattccacc cccgcaataa tcgcacgacg
    41041 aacgccatcg ccaaggaggt taaccaacaa cacgctaatc ataattgccg cacctggcag
    41101 catgacagtc cacggggcga cataaatcag ttccagcgca tcaccgagca tcgctcccca
    41161 ttcaggcgag gggagttgtg cgccgagatc gagaaagccc agcgcggcga tatcgagaat
    41221 tgccatcgac agtgcgcggg tgatctcggt gaccagcccg gcggtgatgt ttggcatcac
    41281 agcaaaccag agaatattca gcgttgatgc gccatccaga cgggcggcga taacgtactc
    41341 tttttccagt tcgtcatgca ccatgctgta aatcgaacgt accatacgcg gcagcaatgc
    41401 cagccagaca gcaaacatag cgtgggacaa actcggtccg gcaaacgcta caacgataat
    41461 tgccaacagc agcgaaggga tcgccagtaa ggtatccaga atatggttaa gcaccgctga
    41521 gcggaggccg tgtgtcgccc cggcgaaggt gcccagtacc aggccacaga tcgtcgcggc
    41581 aagcgtcacc acaaatgcgc cacccacggt tggcgcagct ccgctcagta aacggcttaa
    41641 aacatcacgt ccgaggtcgt cagtccccag gaagaaagaa acttcgccat agcgcgacca
    41701 tgatggcggc aataattgat aaccgagaaa ttgctggtcg atgccgtaag gcgcaaacca
    41761 gccgccaaaa atacacagta cagccagtcc cgcgcagccg tacaggccga ccatcgcaga
    41821 ggcgtcacta taaaatttgc gccaggcggt acgcagcgtg cccggcgggc gcttttcgct
    41881 gtatacgcta tcgtaaggca taccattcct tatgtttcag agggttagcc atggcaccca
    41941 aaatatcaga aatcacgtta acaataatga ccagtgagcc acacaccatc actccggcgg
    42001 aaatggctgc gtaatcctgc tggcggatgg cgttaattaa ccagcgccct aaacccggcc
    42061 agctaaaaac catttcggtg atcatcgcca gcgtcagcat ggtggaaaac tgtaagccca
    42121 ggcgaggaat aaccggcggt aacgcgttat gcagaacgtg gcgacgcaaa atagtaaagc
    42181 gcgacaaacc gcgggtcgcc gccgctttta catagttctg gtcatacact tcaatagtgc
    42241 tgatacgcat caggcggatc acttcagttg ttggcgcaac cgacagggtg atcacgggca
    42301 atatcatatg gcggattgcg ctcatgatca tttcatcccg ccatggtgag tccgaaagcc
    42361 aggcatcaat caacgcaaaa ccggtaatcg gtttcacttc gtagagcaga tcgaaacgcc
    42421 ctgaaacggg caaccaacct agcgtgagtg aacaaaacag tgtcaacaga agcgccagcc
    42481 agaacaccgg gattgaaaag cccagtaagg cgatggcgtt gatcaagtta tcctgccact
    42541 tatggcgcgt aatcccggca atcatcccca cggggatgcc aacaatcagc gcaaaaccga
    42601 aggcgaggat gcacagttct atcgtcgccg ggaagagctc ttttaactgt tcggcaattg
    42661 gttgaccgtt aatgctggaa acaccaaaat cccagtggat caggccatta aaccagaaca
    42721 cccaggcatt ccacagtgac gcgccttgta acggcgcgtg tggagtgaaa tagctcaagc
    42781 tgaagccaac gaaggtcagc aggaacaagg tgacaatcaa taacaaaatg cggcgtaagg
    42841 tgaagataat catggttttt tcacctcatc ctgtttctcg cgatacaccc cagcaaagga
    42901 ggcgttacca aacgggctaa gtaccagacc tttgatatcg taccgatagg cctgcaaacg
    42961 caatgacgac gccagcggca gaataggtaa ttcctgcgcc agaatactct gcgcttcgtc
    43021 ataggcttca atacgcgctg ccagctgctg cgaggagagc gccttacgca acacgctgtc
    43081 gaatttcgga ttgcaccagt gggcgaggtt ggtctgtgaa tgaattgccg cgcagctcag
    43141 taacggacgg aagaaactgt ccgggtcgtt actgtccgtc gcccaaccgg ataacgtcag
    43201 atcatggctc atatccatca accgcgcctc ctgaaagcga ccttctaccg gcacaatcac
    43261 cacttttacg ccaacctgcg ccatatccgc ctgaatcagt tcggcagttt tcagtggact
    43321 ggggttccac gcctgcgaac gtgtgggcac ccacagtttc agcgttaagt tttccagccc
    43381 caacgccttc aactgttcgc gcgatttcgc cggattgtat tcagtaattt tagcctcgtt
    43441 gtcataggcc cacgaggcgc gcggtaaaat agaggccgcc gtttcagccg taccataata
    43501 gatggattgc atcaggcgct ggttattaat cgccagtgcc agtgcatggc ggacagcggg
    43561 attatttagc ggcggtttgg cggtgttaaa tgccagatag gcgacgttca tcccaggacg
    43621 cagcgttaaa cgcaagcgcg ggtcgtcacg caaaatggat agctggctgg cagcaggcca
    43681 ggccagaacg tcgcattccc cggtcaggag tttcgacaga cgtccggtgc cgccggagcc
    43741 taaatccacc actacctgcg gcattaacgg tttaccgcgc cagaagtcat catgacgttg
    43801 taggcgaata aattgcccgg cgcggtattc cgacaactga tacggaccgg tgccgaccgg
    43861 ttgacggtcg agttgctcct ggcgatcttc tttctctaac ttccgggcat attctgccga
    43921 catgaccgaa gcataatggg ttgcgaggtg ccacaaaaaa gaagcatccg gctgggcgag
    43981 tcggaactca acggtatgat tatccagttt gcggacgctt ttcacgttat cggcaaattg
    44041 caggctgtcg aagtagggga agttgctgcc gttgacgtta tgccacgggt tgttgcggtc
    44101 aaaaattcgc tggaaggtaa acaccacatc gtcggcattc attttacgag tgggagtaaa
    44161 ccagtcggtt ttttgaaacg gaacatcgcg acgcaggtgg aagcgatagg tcgcgccgtt
    44221 gtcgagtact tcccagcttt cggcaagttc cggcatcagg cgataggtat agggatcgac
    44281 atccagcagt cgatcataaa actgggcggc aagggtatcg acaattaacc cactgctcgc
    44341 tttggatggg ttaaaggtgt tgacttgccc gctgacgcaa tagacaaaac cgctgtcgcg
    44401 gatatcagca tgcggggggg attcaggcgc ggcgattgcc tgaccactca caagtccagc
    44461 aatcaccaaa agagacgata atacctggcg cataatatta agggatttta tgtaaagagg
    44521 ctatcttact aatatttaat gacatttgcc attaccgttt gtgttcaggg gtcgtaatga
    44581 gggccgtgtg gttggtcgtt gaacaggtgg cgtcgccatc tgttcaacat tcgtacccgt
    44641 gatttctcta taactataac tcacagaaca acttagcgag gagcagagcg gtacgatcgt
    44701 gagaaaccca ttaaagcacg ctgcagtgcg tgtcattgtt agccggatgc ggcgtgaacg
    44761 ctttatccgg acaacgatac tgaccgatcg tctgcaatta aataattact cattacccca
    44821 ttgattcaaa aactctgcga tctcatcaat gcgtacggga ttaatccccg cttcagcagc
    44881 catttcatgt tgggcttctt cgctgatctc ttcattgttc atcaaacggg tgagcagtaa
    44941 ctggaagtag tgggccagcg gtgtgcgttc agcctctgcg acaggctctg cgcattcggt
    45001 cgcgtactca tcggcaatat caaaatattt tagcgggata tcgtggttca ttatttgccc
    45061 ctggggttaa tgcgctaagc ataagggcgg atgatagcac tcgtgatccg ggtcatacat
    45121 ctttttatag ttaatggcgc ggcgcgttac cctcaggcag gataatgcgc cgcgcatccg
    45181 gctattacgt ttcgctcacc ggcgttctgt caccttcgta cagcggcact ttgcgatagc
    45241 ggcggatcag ataccacaaa tatgcgccgc ctaacgaagc ccataccaga ccgagtgtca
    45301 gtgacgttga ctctaggtta acccacagca cacccaccgt cagcgcgcca accagcggca
    45361 tcagcaaata gtggaagtga tccttccagc ttttattcat tcctttacgc cgccagaaat
    45421 gattaaacac cgacagattc acgaaggtaa acgccaccag agcaccgaag ttaatcagtg
    45481 ctgtcgcggt gactaaatca aagaacagcg ccgacagcgc gacaatcccg accataatga
    45541 cgttgagtgc cggagtccgc cattttgggt gcacatagcc aaacacgcgc tccggaaaca
    45601 cattgtcacg ccccatcaca tacagcagac gcgacacgct ggcatgtgaa gccaggccag
    45661 acgctaatgt gttcacaaac gtggtgcaga ggaaaatcga ctgaaagagc ttgccgccga
    45721 cgtacagcgc aatttcaggc agtgcggcgt ccgggtcttt aaagcggctg atatcgggaa
    45781 agaacagctg cataaagaac gacgccgcga taaagataac gccgccgtat accgccgtca
    45841 ggaagatggc tttcgggatc acgcgtgcgg cgtccggcgt ctcttccgaa agcgtggtca
    45901 ccgcatcgaa accgaggaac gagaaacaga caatagtcgc accggtaata atcgggatca
    45961 ggtgcgcgtt ctcgctgata aacggctgaa gtgaccagac ggtgccaacg ccttctcctt
    46021 tatgcagtcc ctgaaccacc aggaagataa acaccaccat gatggagatt tgcaccagta
    46081 caaacagggt attgaagtta gcgaccaggt tgacgctctt cagattcgcg gcggttaaaa
    46141 tggcgacgaa ggttaccacc cacacccacg gcggcacttc cgggaagagg gcggagagat
    46201 agattttcgc caacaaaacg ttgatcattg gcaaaaagag ataatccagc agtgatgacc
    46261 agccgaccat aaatccgacg tgcgggttaa tcgacttttg cgcgtaggta taggccgaac
    46321 cggcctccgg aaactggcga accagtttgc cgtagctgat agcggtaaac agcacgcccg
    46381 ccagcgccag caaataggac gccggaacgt gaccgtcgct aatgccggag acaataccaa
    46441 aagtatcaaa tacggtcatc ggcgtgagat aggccagacc catcattacc acctgccaca
    46501 atttcagtga ttttcgcaga cgggttttgc cgggttgcgc agcaatattc agtggtgaat
    46561 taatagccat aattgctgcc tcccctgcgt aataaggttt gcggtcgtgt cgacctgaaa
    46621 tcaaacctgt ttagcaaggg aagcaaaggg ggcggagtcc agtcagtagc aggaggggcg
    46681 catacgcagc ctccctcgcc atgcggggat ttaatcatcg gcatcatctc atttcctcgt
    46741 cacagtctta atcgttaacg gaagcccgtt gccgcctgcg ctccataacg cgacatccgg
    46801 gaaatgtctg gattaccagc aaatgtcagg cctggctccg ctcaggccga tgaaacaacc
    46861 ccgcaagggg aattacgcgt ttttcaacat ccactcaatt tctgtttctg tgatgaggcg
    46921 ctcaaactgc aacagctcat catttttaca ggcgtgatac acatggcaaa accgttcgcc
    46981 taaatagcgg cgcaggtgat cattctcgat aaactcaccc agggcatcgc tctggcgaat
    47041 cgggaagggt aagccttcct gttccagccc gttgccttcg acttcttcct gcaacggcag
    47101 ctcgttatca aggccatgca aaataccggc aaaaatcgct gccatcacca gatatgggtt
    47161 ggcatcggca cccgccacgt gatattccac gcggtgatta tgacggtcac cgcacggaat
    47221 acgcagggcg acggtgcggt tgttatggcc ccacgacgcc tgcgtcggca catacattcc
    47281 cggctggaag cggcgatacg agttcacgtt tggtgccagc aacgccatcg acgacggcat
    47341 caggtcaatc atcccggcga gcatcttttt cagcagcggc gaatcttcgc cttctgtgtc
    47401 agaaagcaca ttctcgccac gattattttg catactgata tggatatgca tcccgctgcc
    47461 cgcgtgctct tcatacggct tcgccataaa agtggcgtgc atcttatgct tttctgccat
    47521 cagacgcact aggcgtttta gtgccagcgc atcatcgcag gcttccagca cgttatcagt
    47581 atggtaaagg ttgatttcaa actgacccgg cgaggcctca gcgaccgcgc catctgccgg
    47641 aatcagctgc aactgcgcca gttcatcaat atcattgagc acgtcggcga agtggttgag
    47701 gttatcaacg gagtaaacct ggctttgtgt attgcggtca tcggtgccag gcgcgcaggg
    47761 cggttgcaga tccccttcag cgtcgcgctg gcgatccagt aaatagaact ccagctctac
    47821 cgctacaacc gggaacaatc cgcgctggcg cagctgctgc cacaggcggt taagaacgtt
    47881 ccgcggctca acgtcaaagg gagcgccatc ttcatcgacc atggtcagga gcatctgacc
    47941 gataaactct ggatcggcgg cagaaggagt taaggaaccg agaacaggaa cacaggtacg
    48001 atccggctcg cccatttcct gacccagacc tgcttcttct actacgttgc ccagaatatc
    48061 cattgcaaat accgaggccg ggaaataaca ccctttctcg agcttcttca ggcttgaaac
    48121 agggatacgt tttccacgga agcaaccatt caaatcagta agtaaaacat caacatattg
    48181 cgtattcggg tagcgctcca ggtagcgttt cacttcctgc gtaaaggcgc taccccgcct
    48241 ctcttctgac tgctgaacaa agttctctac ttcaacgata ttggtttcca tgattcttcg
    48301 cctttggttt gttttccgct cgttatcaaa gcgtaaaata taatgaccat cattcgaatc
    48361 tgtatgcaaa ccaaatgttt gtcaaatgtt aaattgagtt tgcaaaaatg aaaatccact
    48421 gctagattga aaaaatattg aacataaagg tcatttaaag cgcagtaatg gcgataattt
    48481 agtccatttt gtgagattga gcatggaaaa tataatgaac aatccggtta tcggtgtcgt
    48541 aatgtgcagg aacaggctta agggtcatgc gacccagact ctgcaagaaa agtacctgaa
    48601 tgccatcatc cacgcaggcg gcttgccaat tgcgctacca catgcgctgg cggaaccgtc
    48661 gttacttgaa caacttttgc cgaaactcga tggcatttat cttcctagta gtcccagcaa
    48721 tgtgcagccg cacctatatg gtgaaaacgg cgatgagcct gacgccgatc ccgggcgtga
    48781 tcttctgagc atggcgataa ttaatgccgc actcgaaagg cgcatcccca ttttcgccat
    48841 ctgccggggt ttacaagaac tggtggtggc aaccggtggt tcgttgcatc gcaagctgtg
    48901 cgaacagcct gaattgctgg aacatcggga agatcccgaa ctgccggtgg aacagcaata
    48961 tgcaccgtcg catgaagttc aggttgaaga ggggggatta ctgtctgcgt tgttacctga
    49021 atgtagcaac ttttgggtaa actctctaca tggacaaggg gcgaaggtcg ttagcccacg
    49081 gttgcgtgtt gaagctcgct cgccggatgg cttagttgag gcggttagcg tcatcaatca
    49141 tccttttgcg ctgggcgtac agtggcaccc ggaatggaac agtagcgagt acgcgctttc
    49201 gcgtatattg ttcgagggct ttatcaccgc ttggcagcac catatcgctg aaaaacagcg
    49261 actctgacca ctacagttta aggaaatgca aatatgagtg atgagggact ggcgccagga
    49321 aaacgcttgt cggaaatccg ccagcagcag gggctttcac aacgtcgtgc cgccgaactc
    49381 tccgggctga ctcacagtgc tatcagtacg atagaacaag ataaagtcag ccctgccatc
    49441 agtacgctgc aaaagctgct gaaggtgtat ggtctgtcac tctcggaatt cttttccgag
    49501 ccggaaaaac ctgatgagcc gcaggtcgtc attaatcagg acgacttaat tgagatgggc
    49561 agtcagggtg tgtcaatgaa gctggttcat aacggtaacc cgaatcgcac gctggcgatg
    49621 atctttgaaa cgtaccagcc gggcacaacc actggggaaa gaattaagca tcagggtgag
    49681 gaaataggca cagtactgga aggtgaaatt gttctgacga ttaatggtca ggattaccac
    49741 ctcgtcgcgg ggcaaagtta tgccattaat accggcatcc cgcacagttt cagtaatacg
    49801 tcggcaggta tttgccgaat tatcagcgcc catacgccca ccacgtttta atctttttgt
    49861 tctgtaagcc gggtaagcgc aatgcgcccg gcaatccata aagaatgtcg ccagaaataa
    49921 acgggcatgc agcccgggga tctctgcgcc ctgacgttca caaactgcat atatctgata
    49981 gacgtgaaac aggagtcata atgaattttc atcatctggc ttactggcag gataaagcgt
    50041 taagtctcgc cattgaaaac cgcttattta ttaacggtga atatactgct gcggcggaaa
    50101 atgaaacctt tgaaaccgtt gatccggtca cccaggcacc gctggcgaaa attgcccgcg
    50161 gcaagagcgt cgatatcgac cgtgcggtga gcgcagcacg cggcgtattt gaacgcggcg
    50221 actggtcact ctcttctccg gctaaacgta aagcggtact gaataaactc gccgatttaa
    50281 tggaagccca cgccgaagag ctggcactgc tggaaactct cgacaccggc aaaccgattc
    50341 gtcacagtct gcgtgatgat attcccggcg cggcgcgcgc cattcgctgg tacgccgaag
    50401 cgatcgacaa agtgtatggc gaagtggcga ccaccagtag ccatgagctg gcgatgatcg
    50461 tgcgtgaacc ggtcggcgtg attgccgcca tcgtgccgtg gaacttcccg ctgttgctga
    50521 cttgctggaa actcggcccg gcgctggcgg cgggaaacag cgtgattcta aaaccgtctg
    50581 aaaaatcacc gctcagtgcg attcgtctcg cggggctggc gaaagaagca ggcttgccgg
    50641 atggtgtgtt gaacgtggtg acgggttttg gtcatgaagc ggggcaggcg ctttcgcgtc
    50701 ataacgatat cgatgccatc gcctttaccg gctccacgcg tacgggtaaa caactgctga
    50761 aagacgcggg tgatagcaac atgaaacgtg tctggctgga agcgggcggc aaaagcgcca
    50821 acatcgtttt cgccgattgt ccggatctgc aaaaagcggc aagcgccacc gccgcaggca
    50881 tcttctacaa ccagggccag gtgcgcatcg ccggaacgcg tttgttactg gaagagagca
    50941 tcgccgatga attcttagcc ctgttaaaac agcaggcgca aaactggcag ccgggccatc
    51001 cacttgatcc cgcaaccacc atgggcacct taatcgacag cgctcacgct gactcggtac
    51061 acacctttat tcgtgaaggc gaaggcaagg ggcaactgct gctggatggt cgcaacgccg
    51121 ggctggcagc ggccatcggc ccgaccatct tcgtggatgt ggacccgaat gcgtccttaa
    51181 gccgcgaaga gattttcggt ccggtgctgg tggtcacccg tttcacatca gaagatcagg
    51241 cgctgcagct tgccaacgac agccagtacg gccttggcgc ggcggtatgg acgcgcgacc
    51301 tctcccgcgc gcaccgcatg agccgacgcc tgaaagccgg ttccgtcttc gtcaataact
    51361 acaacgacgg cgatatgacc gtgccgtttg gcggctataa gcagagcggc aacggtcgcg
    51421 acaaatccct gcatgccctt gaaaaattca ctgaactgaa aaccatctgg ataagcctgg
    51481 aggcctgaaa tgaccgaaca taccagcagt tactacgccg ccagtgcgaa taaatatgca
    51541 ccattcgaca cactgaatga gtcaatcacc tgcgacgttt gcgtggttgg cggcggctat
    51601 accgggctct cctccgcgct gcatctggcg gaagcgggct ttgacgtagt ggttctcgaa
    51661 gcctcacgca tcggctttgg cgcaagcggg cgcaatggcg ggcagcttgt gaactcctac
    51721 agccgcgaca tcgacgtgat cgaaaaaagc tacggcatgg acaccgcccg tatgctcggc
    51781 agcatgatgt tcgagggtgg cgagatcatc cgcgaacgta tcaaacgtta tcagattgac
    51841 tgcgactacc gccccggcgg tctgtttgtg gcgatgaatg ataaacaact cgccacactc
    51901 gaagagcaga aagagaactg ggtacgctac ggcaataaac agctggagct gctggacgtc
    51961 aacgctattc gccgcgaggt cgccagcgat cgctacactg gtgcgctgct ggatcacagc
    52021 ggtgggcata ttcacccgct aaacctcgcc attggtgaag ccgatgccat ccgcctgaac
    52081 ggcgggcgcg tgtatgagct ttccgccgtg acgcagatcc agcacaccac gccagccgtg
    52141 gttcggactg ccaatggtca ggtgacggcg aagtatgtga ttgtcgccgg gaatgcgtat
    52201 ctgggcgata aagtagagcc ggaactagcg aaacgcagca tgccgtgcgg cacgcaggtg
    52261 atcaccaccg aacggctgtc ggaagattta gcccgttcgc tgatcccgaa aaactactgt
    52321 gtggaagact gtaactatct gcttgattac taccgtctta ccgccgacaa ccgcctgctg
    52381 tacggcgggg gtgtagtgta tggtgcacgc aacccggatg acgttgagcg tcttgtggta
    52441 ccgaaactac taaaaacctt ccctcagttg aagggcgtga aaattgatta ccgctggacg
    52501 ggcaacttcc tgctgacctt gtcgcacatg ccgcagttcg gccgccttga taccaacatc
    52561 tattacatgc agggttacag cggccacggc gtcacctgta cccatcttgc cgggcgtttg
    52621 attgctgaac tgctgcgcgg tgacgccgag cgtttcgatg ccttcgccaa tctgccgcat
    52681 tacccgttcc ccggcgggcg cacgctgcgc gtgccattta ccgcgatggg cgcggcttat
    52741 tacagcctgc gcgatcgtct gggcgtttaa tttccgatta acagtgaaga gtcaaaaggt
    52801 gtgaaacatg agcaacaatg aattccatca gcgtcgtctt tctgctaccc cacgcggggt
    52861 tggcgtgatg tgtaacttct tcgcccagtc ggcagaaaac gctacgctga aggacgtaga
    52921 gggcaacgaa tacatcgatt tcgccgcagg cattgcggtg ctgaataccg ggcatcgcca
    52981 ccctgatctg gtcgcggcgg tggagcagca attgcaacag tttacccaca cggcgtatca
    53041 gattgtgccg tacgaaagct acgtcacgct ggcggagaaa attaacaccc ttgccccagt
    53101 gagcgggcag gctaaaaccg cattcttcac taccggcgcg gaagcggtgg aaaacgcggt
    53161 gaaaattgct cgcgcccata ccggacgacc tggcgtgatt gcgtttagcg gtggcttcca
    53221 cggtcgtaca tatatgacca tggcgctgac cggaaaagtt gcgccgtaca aaatcggctt
    53281 cggcccgttc cctggttcgg tgtatcacgt accttatccg tcagatttac acggcatttc
    53341 aacacaggac tccctcgacg ccatcgaacg cttgtttaaa tcagacatcg aagcgaagca
    53401 ggtcgcggcc attattttcg aaccggtgca gggcgagtgc ggtttcaacg ttgcgccaaa
    53461 agagctggtt gccgccattc gccgcctgtg cgacgagcac ggtattgtga tgattgccga
    53521 tgaagtgcaa agcggctttg cgcgtaccgg taaactgttt gccatggatc attacgccga
    53581 taagccggac ttaatgacga tggcgaaaag cctcgcgggc gggatgccgc ttt
//
LOCUS       DAJTSF010000015        52597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000015 DAJTSF010000000
VERSION     DAJTSF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.015"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..354
                     /locus_tag="OO335_000977"
     CDS             <1..354
                     /locus_tag="OO335_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR6001459.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO335_000978"
     CDS             complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO335_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6001460.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO335_000979"
     CDS             complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO335_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6001461.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3109..3261
                     /gene="mokC"
                     /locus_tag="OO335_000980"
     CDS             3109..3261
                     /gene="mokC"
                     /locus_tag="OO335_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6001462.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO335_000981"
     CDS             complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO335_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6001463.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO335_000982"
     CDS             complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO335_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6001464.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6877..7281
                     /locus_tag="OO335_000983"
     CDS             6877..7281
                     /locus_tag="OO335_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6001465.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7307..8020
                     /gene="msyB"
                     /locus_tag="OO335_000984"
     CDS             7307..8020
                     /gene="msyB"
                     /locus_tag="OO335_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6001466.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8169..8735
                     /gene="satP"
                     /locus_tag="OO335_000985"
     CDS             8169..8735
                     /gene="satP"
                     /locus_tag="OO335_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6001467.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO335_000986"
     CDS             complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO335_000986"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6001468.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO335_000987"
     CDS             complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO335_000987"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6001469.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10704..12134
                     /locus_tag="OO335_000988"
                     /pseudo
     CDS             10704..12134
                     /locus_tag="OO335_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12204..12980
                     /gene="yaaA"
                     /locus_tag="OO335_000989"
     CDS             12204..12980
                     /gene="yaaA"
                     /locus_tag="OO335_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6001470.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13133..13429)
                     /locus_tag="OO335_000990"
     CDS             complement(13133..13429)
                     /locus_tag="OO335_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6001471.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="OO335_000991"
     CDS             complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="OO335_000991"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6001472.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="OO335_000992"
     CDS             complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="OO335_000992"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6001473.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="OO335_000993"
     CDS             complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="OO335_000993"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6001474.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18352..18471)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="OO335_000994"
     CDS             complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="OO335_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6001475.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18686..19372)
                     /locus_tag="OO335_000995"
     CDS             complement(18686..19372)
                     /locus_tag="OO335_000995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6001476.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19772..19912)
                     /locus_tag="OO335_000996"
     CDS             complement(19772..19912)
                     /locus_tag="OO335_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001477.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20008..20724
                     /gene="arcA"
                     /locus_tag="OO335_000997"
     CDS             20008..20724
                     /gene="arcA"
                     /locus_tag="OO335_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6001478.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20783..22135)
                     /gene="creD"
                     /locus_tag="OO335_000998"
     CDS             complement(20783..22135)
                     /gene="creD"
                     /locus_tag="OO335_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6001479.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22193..23617)
                     /gene="creC"
                     /locus_tag="OO335_000999"
     CDS             complement(22193..23617)
                     /gene="creC"
                     /locus_tag="OO335_000999"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6001480.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23617..24306)
                     /gene="creB"
                     /locus_tag="OO335_001000"
     CDS             complement(23617..24306)
                     /gene="creB"
                     /locus_tag="OO335_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6001481.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24319..24792)
                     /gene="creA"
                     /locus_tag="OO335_001001"
     CDS             complement(24319..24792)
                     /gene="creA"
                     /locus_tag="OO335_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6001482.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25003..25872
                     /gene="robA"
                     /locus_tag="OO335_001002"
     CDS             25003..25872
                     /gene="robA"
                     /locus_tag="OO335_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6001483.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="OO335_001003"
     CDS             complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="OO335_001003"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6001484.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26568..27088
                     /gene="yjjX"
                     /locus_tag="OO335_001004"
                     /pseudo
     CDS             26568..27088
                     /gene="yjjX"
                     /locus_tag="OO335_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="OO335_001005"
     CDS             complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="OO335_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6001485.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="OO335_001006"
     CDS             complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="OO335_001006"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6001486.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29737..31404
                     /gene="ettA"
                     /locus_tag="OO335_001007"
     CDS             29737..31404
                     /gene="ettA"
                     /locus_tag="OO335_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6001487.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31511..31678)
                     /locus_tag="OO335_001008"
     CDS             complement(31511..31678)
                     /locus_tag="OO335_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001488.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="OO335_001009"
     CDS             complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="OO335_001009"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6001489.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32965..34347)
                     /gene="radA"
                     /locus_tag="OO335_001010"
     CDS             complement(32965..34347)
                     /gene="radA"
                     /locus_tag="OO335_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6001490.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34396..35364)
                     /gene="serB"
                     /locus_tag="OO335_001011"
     CDS             complement(34396..35364)
                     /gene="serB"
                     /locus_tag="OO335_001011"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6001491.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35470..36114
                     /locus_tag="OO335_001012"
     CDS             35470..36114
                     /locus_tag="OO335_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6001492.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36142..37157
                     /gene="lplA"
                     /locus_tag="OO335_001013"
                     /pseudo
     CDS             36142..37157
                     /gene="lplA"
                     /locus_tag="OO335_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37189..37452
                     /locus_tag="OO335_001014"
     CDS             37189..37452
                     /locus_tag="OO335_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6001493.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37613..38332)
                     /gene="deoD"
                     /locus_tag="OO335_001015"
     CDS             complement(37613..38332)
                     /gene="deoD"
                     /locus_tag="OO335_001015"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6001494.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38389..39612)
                     /gene="deoB"
                     /locus_tag="OO335_001016"
     CDS             complement(38389..39612)
                     /gene="deoB"
                     /locus_tag="OO335_001016"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6001495.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39664..40986)
                     /gene="deoA"
                     /locus_tag="OO335_001017"
     CDS             complement(39664..40986)
                     /gene="deoA"
                     /locus_tag="OO335_001017"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6001496.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41113..41892)
                     /gene="deoC"
                     /locus_tag="OO335_001018"
     CDS             complement(41113..41892)
                     /gene="deoC"
                     /locus_tag="OO335_001018"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6001497.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42150..43691
                     /locus_tag="OO335_001019"
     CDS             42150..43691
                     /locus_tag="OO335_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6001498.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43663..44526
                     /locus_tag="OO335_001020"
     CDS             43663..44526
                     /locus_tag="OO335_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6001499.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44740..45522)
                     /locus_tag="OO335_001021"
     CDS             complement(44740..45522)
                     /locus_tag="OO335_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6001500.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45519..46592)
                     /locus_tag="OO335_001022"
     CDS             complement(45519..46592)
                     /locus_tag="OO335_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6001501.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46714..46875)
                     /locus_tag="OO335_001023"
     CDS             complement(46714..46875)
                     /locus_tag="OO335_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6001502.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46992..47597)
                     /gene="osmY"
                     /locus_tag="OO335_001024"
     CDS             complement(46992..47597)
                     /gene="osmY"
                     /locus_tag="OO335_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR6001503.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47990..49579)
                     /gene="prfC"
                     /locus_tag="OO335_001025"
     CDS             complement(47990..49579)
                     /gene="prfC"
                     /locus_tag="OO335_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6001504.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49670..50347)
                     /gene="yjjG"
                     /locus_tag="OO335_001026"
     CDS             complement(49670..50347)
                     /gene="yjjG"
                     /locus_tag="OO335_001026"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6001505.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50362..50808)
                     /gene="rimI"
                     /locus_tag="OO335_001027"
     CDS             complement(50362..50808)
                     /gene="rimI"
                     /locus_tag="OO335_001027"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6001506.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50777..51190)
                     /gene="holD"
                     /locus_tag="OO335_001028"
     CDS             complement(50777..51190)
                     /gene="holD"
                     /locus_tag="OO335_001028"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6001507.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51293..52324
                     /gene="rsmC"
                     /locus_tag="OO335_001029"
     CDS             51293..52324
                     /gene="rsmC"
                     /locus_tag="OO335_001029"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6001508.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12526 a        14234 c        13181 g        12656 t
ORIGIN      
        1 tatcagtttc atcatataag atttgactgc atccagggcg agcaaggtga attagttttt
       61 gaacccagtg acgttgaata ttactttgaa ccagtgacga tacaggagtc aggaaccagc
      121 atattaaaaa atgagttaga aaacagtgag gacggtgcgg gtcaaatagg ctttcaactc
      181 tccaatgatg gtacgaatga aatccagtat ggtaaaagca attactactc ttttcagcat
      241 ccccatgaag gaagtaatca gattcctcta tttattcgcc cgcgtacata tggaaacaat
      301 gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg taatgtacaa ctaatctatc
      361 tcccccacct taactttggg ggagctgccg attaacgcac cgctggacta aaaagcgcag
      421 aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa
      481 tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg
      541 cataaaagtc atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa
      601 aagctttcat caaagcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg
      661 agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc
      721 gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac
      781 tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg
      841 gacagtcaga aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt
      901 gggtggattc gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta
      961 cgctactgac caggcgtttg gaaagtgcat cagccacgcc aacgtcaaac aataaattgg
     1021 attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg
     1081 catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa
     1141 ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag
     1201 ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt
     1261 aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa
     1321 agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac
     1381 gcgtaaccag ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt
     1441 cgcccagtta atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga
     1501 catagtaaaa ccgataccgc acaggatccc caccaccata atttgctgat aagtcgttcc
     1561 ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag
     1621 cggtttgcca atcagcaagc cagcgatgat ccccaatggc agaatggagg tcaagccatc
     1681 cagcgtgacg ccttgcagtg aaacgccagc attagcaaat gcaaacagcg gcaaaatcag
     1741 atacgccacc cacgggtgca acacatgctc cagtcgcttc gctggagaac gcccatgctt
     1801 ctctttcaaa ggaataaaga agccgacaat tacccccgcc agagttgcgt gaacccccga
     1861 tttcaacacc gcagtccaca acaccacgcc aacaagaata tagacgcccg tgcggcgtac
     1921 accacacaga ttcaataccg cgagtaccgc aattgctaca gccgcgacgc caagggaggc
     1981 catcgataag tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat
     2041 aatagccaga gccatcaaaa agatcttcag cactaacgga acacgacttc ccaacagcgc
     2101 cagtacacca agtgcaaaag caatgtcagt agccgccggg atcgcccacc cttcgcgggt
     2161 aatcggatcg gcatagttaa aagccagata gagtaatgcc ggcacaatca tcccaccaat
     2221 agcggcgata actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc
     2281 acgtttaact tccagaccga ctaacaggaa aaataccgcc atcagcgcat catttatcca
     2341 taacagcatg tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag
     2401 aaagtcgtga taccatccac tggttgcgcc gctgttggcc ataatcatcg ccaggatagc
     2461 ggcaatgata agaataatgc ctcccgaggc atcactgcta aagaatcgat gcagatgttt
     2521 cactttttat ttctctttca ggtgaataga tcgtttttac tattttaccc cgcacgaatc
     2581 atcgttaaaa tcagattata gtgtaggaat aattcgtttt ttacgagcca ttgaagaatt
     2641 tgcgccatca gcggtgagat gcgtttttga cggctgtaag cgctcacgag tgaccctaag
     2701 cccagataat catagcttaa ggtgaggatt cctgagggat taattaggaa aagtaataat
     2761 taatttgttg tcagtcggcg atgcttgtta ggccaatatt tcaaaaataa tccagcttgt
     2821 cgtcaataat tgcgatgaag agataaatta aagttaaata attaaatcat taaaaaatta
     2881 cgccacaaaa atgcgaggcg tcttcaggat tagaatcgat aaaacaaaat gggttaaaga
     2941 gagcgatata atagcggcgg gtgcttgagg ctgtctgtct caggcattag ctgaacggca
     3001 gatagagaaa agccccgagt gatattttac catcaacccg aggcctccta tatgctgaac
     3061 acatgtagag tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat
     3121 aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggta
     3181 acgagaaaag acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc
     3241 acggcttacg aatccgagta agagcaacgg cggggagtga tccccgccac tttacaggtg
     3301 ctcgcatatc ttcaacgcac cctattttta cccaggcctg cccacgggca ggcttttggg
     3361 gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc
     3421 gcgggctgtt gtgctcgccg gttgggccac cgaagctttc ttgcagctct tgcagcagct
     3481 gtttctgctt ttcgttcagg cctaccggtg tttcgacgac aacgcggcac agcaaatcac
     3541 cctgtgcgcc accgcggaca gacttgacgc ctttaccgcg catacggaat agcttaccgg
     3601 tctgggtttc gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt
     3661 cgccacccag cgccgccata gcgaagttga tcgggacttc gcaatacagg ttgttgcctt
     3721 cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg
     3781 cgccatgctc gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc
     3841 ctgccgggat tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt
     3901 tgttgcacgg atctttgatc agcgtaccgc ggccctgaca gtgtggacag gtctgctgta
     3961 cagcgaagaa tccctggcgc atctgcacct gaccagaacc atgacaggtc ggacaggtct
     4021 gcggctgtgt acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg
     4081 gaatgcggat ctctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat
     4141 agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc
     4201 cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc gccaaaacca ccgccgccca
     4261 tgcctccttg ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt
     4321 cggtcagaac ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac
     4381 cctggttacg gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ctgatttcac
     4441 gctcttccgc tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt
     4501 ttaaattgcc cctagatgaa tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt
     4561 ataattaccc gtttataggg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg
     4621 acaacatcgt catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg
     4681 gcatgttgct gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt
     4741 ttcgcttcga tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagcgca
     4801 gactcgatag cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta
     4861 cgggtgctgt gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta
     4921 cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc
     4981 agaccagaag aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg
     5041 gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg
     5101 cgcggtgccg ggttgatacc atctaggttg aactgaccca gagatttgtt atcagccgca
     5161 cgtttacgtt caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta
     5221 gagaacacct ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc
     5281 acaccgccca tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct
     5341 tttacgtcac cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc
     5401 gggttaacgt ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt
     5461 ggcatacgag tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca
     5521 gcatcctgca gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg
     5581 ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca
     5641 gtgatgtatg gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt
     5701 tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga
     5761 tctttcttga attcttcaac cagatagttg atcagacggc tgtcgaagtc ttcaccaccc
     5821 aggtgggtat caccgttggt tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg
     5881 atttcgataa tagaaatatc gaaagtacca ccccccaggt cataaaccgc gatagtacgg
     5941 ttgccagtgc ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata
     6001 cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg
     6061 ttaaagtatg ccggtacggt gataacagct tcagttaccg gttcacccag gtaatcttca
     6121 gcggttttct tcattttttt cagcacttca gcagaaatct gcggcggtgc cattttctgg
     6181 cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg
     6241 gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca
     6301 aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt
     6361 tcaccatcct gggtataggc aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc
     6421 agcacgcgag gaatggtgcc atccataatc gctacacaag agttggtagt acccaggtcg
     6481 ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt
     6541 gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac
     6601 tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc
     6661 aaaaattttt tgtgcttttg cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt
     6721 gacagcaatt ttattcatat aaagaatgaa tcgcagccaa ggcgttcatc aaaaaattgt
     6781 aataaaaaga aaagattacg tgcctgaatc ttctctttat cagcagtaaa ctagtgggta
     6841 ttcatccccc tacctcttcc cactaagaga atccttatga aatccgtttt tacgatttcc
     6901 gccagcctgg cgattagcct gatgctgtgc tgcacggcgc aggcaaacga ccataaactc
     6961 ctcggcgtca ttgcaatgcc gcgtaacgaa accaacgatc tggcgctgaa acttcctgtt
     7021 tgtcgcattg tgaaacgcat acaactctct gccgaccatg gcgatttaca gttaagtggt
     7081 gcatcggttt atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa
     7141 ataaaagaag ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc
     7201 tgcgtctcaa aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg
     7261 aaaattatcg gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta
     7321 cctgaatgat tcagatctgg attttcttca gcattgtagt gaggaacagt tggcaaattt
     7381 cgcccgattg ctcacccata atgaaaaagg caaaactcgc ctctccagca tactgatgcg
     7441 caacgaactg tttaaatcga tggaagggca tcccgagcaa catcgccgca actggcagct
     7501 gattgccgga gaattacagc attttggtgg cgatagtatc gccaacaaac tgcgcggaca
     7561 cggtaaattg tatcgggcca ttttgctcga tgtttcaaag cgattgaagc tgaaagccga
     7621 caaagagatg tctacgtttg aaattgagca acagttactg gaacaatttc tgcgtaatac
     7681 ctggaagaaa atggacgagg aacataagca ggagtttctg cacgcggtcg atgccagggt
     7741 gaatgagctg gaagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggtgt
     7801 gtcgcatttg ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt
     7861 acttgggcat ggtttgctgc gcggcgcggg gctgggaggc cctgtgggcg cagcattaaa
     7921 tggggttaaa gcggtcagcg gcagcaccta tcgcgtgacg attccagccg tactgcaaat
     7981 cgcctgcctg cgccggatgg ttagcgccac tcaggtctga taccggaaac cggggaaaat
     8041 tttcattttc ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc
     8101 cctgaaaaca ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt
     8161 gaggaattat gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg
     8221 gcatgaccac cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta
     8281 ttcttgccat gggcattttc tacggcggca tcgcgcaaat ttttgctggt ctgctggagt
     8341 acaaaaaagg caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga
     8401 cgctggttgc gattctgctg atgccgaaac tgggtctgac cgatgcgcca aatgcacagt
     8461 tccttggtgt ctacctgggt ctgtggggcg tatttacgct gtttatgttc ttcggcactc
     8521 tgaaaggcgc gcgcgtcctg caattcgttt tctttagcct gaccgtgctg tttgccctgc
     8581 tggcgatcgg taacattgcc ggtaatgccg cgatcatcca ctttgccggc tggattggtc
     8641 tgatctgcgg tgccagcgca atctatctgg cgatgggtga agtgctgaac gagcagtttg
     8701 gtcgcaccgt tctgccgatt ggtgaatccc actaattgtc ttgagatccc cccgctcggg
     8761 gggatttttt tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac
     8821 ttctggtgcc gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc
     8881 aaaaatacca tgcaccacaa cgttaccctc agcgtccttc acaccttcca gcgtctcttt
     8941 aatagacttc ggctgaccgg gtaagttaag gatcagcgcc tgtttgcgaa tcacgcccac
     9001 ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctg atctggcgca tctgttcacc
     9061 aaagccaggc atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg
     9121 cgccgggcca gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccaccagctc
     9181 acacaacgtt tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa
     9241 cggcgtggtt agcgccgatg tcagccattc ttccagcgct ggaatgcctt tatcctgata
     9301 aacgccgctg gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa
     9361 gatttttccg tttaaacaat ctcgttaaac agaatgatac actgcgaagg gagtgacaga
     9421 caggcttcag aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag
     9481 atcgccgatc attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc
     9541 cagtttatct actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat
     9601 acgcgccgga cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc
     9661 gctctccgcc agctctttca gcagtgccgg tgcgatggtc agacggtcac agcctgccag
     9721 ttccagaatt tcgccgatgt tacggaagct tgcgcccata accacggttt cataaccgtg
     9781 ctctttgtag tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc
     9841 gtactctttc ttatcggtat tcgctttgta ccagtcaaga atacggccaa caaacggcga
     9901 gatcaggaac acgcccgcct ccgcacaagc acgagcctga gcgaaggaga acagcagggt
     9961 caggttacag ttgatacctt ctttttccag ctgttctgca gcacggatac cctgccaggt
    10021 agaagccagt ttgatcagaa tacgatcgtt gctaatacct gcatcgttgt agagtttgat
    10081 caggcgtttt gctttcgcaa ttgacgcttc agtgtcatag gaaagacgcg catcaacttc
    10141 agttgagata cggcccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt
    10201 gtcggtcgcg tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac
    10261 agcatcatca atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg
    10321 gttggttgtg gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc
    10381 cactacggtg gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt
    10441 taaacagctt gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca
    10501 gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc
    10561 gttgatatcg cccaccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag
    10621 tacctcgtag cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc
    10681 gaccggcaat aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt
    10741 ggggatcggt aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg
    10801 gatttgtgca gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc
    10861 cacagccagg cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg
    10921 gtagcggcaa cctggccggc gttgcgctgg caattaccgc cggtggacca ggtgccgtct
    10981 tttggatgtg ggttgctgcg tttatcggca tggcgacctc gtttgccgaa tgttcccttg
    11041 cacaacttta taaagaacgt gacgtcaatg ggcagtttcg tggcggaccg gcatggtata
    11101 tggcgcgcgg gctggggatg cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg
    11161 cctacggcat aattttcagc ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt
    11221 cttttgattt tcctccgctg gtgacgggca ttattctcgc tgtctttgct ctgctggcaa
    11281 tcactcgtgg tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga
    11341 taatctgggt actgaccagc ctggtgattt gcgtaataaa tatcgggcaa cttccccacg
    11401 tcatttggtc tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg
    11461 gatatacctt aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg
    11521 tgggaatggg atcgacgcca aacgcggcag cggcagctgc atcctggcct ccgcatccgg
    11581 cagcacaagg gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg
    11641 caagcgccat gctgatatta ctggcgggta acggcacaac ctacatgccg ctggaaggta
    11701 ttcagcttat ccagaaggcg atgcgggtgc taatgggttc ctagggtgct gaatttgtta
    11761 ccctcgtggt tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa
    11821 acaatctctt ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca
    11881 ccttcgcaac ggtcatcggc ggcaccttgc taagccttcc gctgatgtgg caactggcag
    11941 atatcataat ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg
    12001 tggttcatac cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt
    12061 ttgatccgtt gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg
    12121 tttcgcagga gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca
    12181 taaatttcct gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt
    12241 accaaagccc gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc
    12301 agttgatcca tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca
    12361 gcgacaaact ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc
    12421 cggcgaatgc ccgccaggcg attctggcgt ttaaaggtga tgtctacacc ggcttgcagg
    12481 ccgaaacctt cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg
    12541 gcttgtatgg cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga
    12601 tccgtcttga gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca
    12661 acaagctgaa cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag
    12721 atgagtattt taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt
    12781 tcctcgatga gaagaacggc aaatttaaga tcatcagctt ctacgctaag aaagcacgcg
    12841 gtctgatgag tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt
    12901 ttaatagcga aggttacttc tttgatgaag attcctccag caatggcgaa ctggtgttta
    12961 aacgctacga gcagcgttaa gtttgtagtc aatgccggat gcggcgtgaa cgccttatcc
    13021 ggcctacaaa agcatgcaga ttcaatatat tgcagaatta acgtaggcct gataagacgc
    13081 gccagcgtcg catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt
    13141 gatgtttgcc tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg
    13201 gcggtggcgg cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc
    13261 accagccgtg gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc
    13321 ctatctgtaa ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag
    13381 cgaccagaac cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct
    13441 tttatcattg cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt
    13501 attctccgca atcctaatct ctaagttacc gcatttattg ataatttctc ctttttccct
    13561 gtcgtttttc tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc
    13621 tgattgagat aatgaataga ttttactgat gattcatcat caatttacgc aacgcagaaa
    13681 aatcggcggg cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg
    13741 gcagatccaa cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg
    13801 cggtgccgag gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg
    13861 ctacggcagc gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt
    13921 gcgtggtttc atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc
    13981 ggaacaactc ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata
    14041 acgtcgcctg agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat
    14101 cgttcacgtt ggtcgcagca ataaaacgtt tcaccggcag accgaatgac ttcgccagca
    14161 gacccgccgt caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg
    14221 cctcctgcgg cagctgcgca acagcttcaa agtagtagca aatctgcgcc agcaaacggc
    14281 tgatgttaat cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa
    14341 acgcctgctt caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga
    14401 tattgccgcc caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat
    14461 agaggataac cactttcaca ttcggcaaac cgtagaaagc atgagccact gcagctccgg
    14521 tatcaccgga ggtcgcggtc agaatggtca ctggcttatc atccgcaata tgggtcagca
    14581 tttgtgccat aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt
    14641 ccagacaacc gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc
    14701 gcacgcgctc ttccaggatt tcctgtggga tttcatcacc aataaacgcc gagaggatct
    14761 tcgcactgcg ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt
    14821 ccggcaggtc gtgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt
    14881 gcgcaaagct gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca
    14941 gtactcgtgc gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca
    15001 ggtagttctt acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag
    15061 cgaacaaggt cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg
    15121 cctgccgcgc ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat
    15181 ctttcatcag cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag
    15241 ccagatgtcg cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc
    15301 gggcttctgc cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa
    15361 accctggcac ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa
    15421 gaaaacacgg tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc
    15481 ccatcaaagc cagcaaacga gtgtcattaa gcggcttgcc gcagtgttca ttcatcgcca
    15541 tcagcgccgc gaccaccgaa caggcgctgg agcctaagcc cgaaccgatc ggcatattct
    15601 tttccagagt catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc
    15661 actgataaac gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgagtt
    15721 tgttgagact gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat
    15781 caacgggtgc caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag
    15841 ccggggcata aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga
    15901 tcggcaaaga caccggcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac
    15961 ggcagcggct gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg
    16021 aacagcggat cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca
    16081 atattgccaa catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca
    16141 aagagatcgt cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta
    16201 aactctgcgg gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt
    16261 tcacgagcga gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg
    16321 tccggttcgg tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct
    16381 tcgtctaact tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc
    16441 aattcatcac ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca
    16501 acgttggtgt catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga
    16561 tagtaatcca tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg
    16621 cgcaggaagt cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc
    16681 gggttcagca gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct
    16741 ttggcttgtg caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc
    16801 agagccttcg agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag
    16861 ctttgctgac gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca
    16921 aacacttcga taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg
    16981 gtcgcatcat cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg
    17041 gcgacaatgt tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc
    17101 aaggtgcgca taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc
    17161 ggctccagta agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct
    17221 cgcacacagt cgctttgtgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta
    17281 atcagcacca cggaaatacg ggcgcgtgac attgcagcaa agacgcgcgc cgccatgcca
    17341 accatccctt tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa
    17401 atgcccttga ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct
    17461 tgaggatttc cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg
    17521 gtgcgggggt gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac
    17581 atcgacttca acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca
    17641 acgtccgtcc aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag
    17701 tagtcggaac cgttacgtcc aagcaccacc agttcgcctt tttcattacc ggcggtgaaa
    17761 cctgccatca acaccatgtg atcagccgga atgcggcttg ccgcaatacg gcgggtggac
    17821 tcagcaatat cgacggtaga ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga
    17881 tcgataacgg taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac
    17941 attttctcgc cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa
    18001 ctaatgccat gcaggacatg ttttatttgg gcaaattcct gatcgacgaa agttttcaat
    18061 tgcgccagcg ggaaccccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata
    18121 cgttcggcat cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc
    18181 gccaccaggt ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc
    18241 ctggcattgc tttccagaat atcggcaaca cgcagaaaac gttctgcatt tgccactgat
    18301 gtaccgccga acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa
    18361 gcccgcactg tcaggtgcgg gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt
    18421 tacctgtggt aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc atggatgttg
    18481 tgtactctgt aatttttatc tgtttgtgcg ctatgcctat attggttaaa gtatttagtg
    18541 acctaagtca ataaaatttt aatttactca cggcaggtaa ccagttcaga agctgctatc
    18601 agacactctt tttttaatcc acacagagac atattgcccg ttgcagtcag aatgaaaagc
    18661 tgaaaaatac ttactaaggc gttttttatt tggtgatatt tttttcaata tcatgcagca
    18721 aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc caggcgttgc tgtaaccagt
    18781 cgaccagttt tatgtcatct gccactgcca gagtcgtcag caatgccatg actcgttcgc
    18841 gtaaagcttg cagttgatgt tggtctgccg ttgtgtcact tttcgccggt tgttgtatta
    18901 atgttgctaa ttgatagcaa tagaccatca ccgcctgccc cagattgagc gaaggataat
    18961 ccgccaccat cggcacacca gtaagcacgt cagccaacgc taactcttcg ttagtcagcc
    19021 cggaatcttc gcgaccaaac accagcgcgg catggctcat ccatgaagat ttttcctcta
    19081 acagcggcac cagttctact ggcgtggcgt agtaatgata tttcgcccga ctacgcgcag
    19141 tggtggcgac agtgaaatcg acatcgtgta acgattcagc cagtgtcggg aaaactttaa
    19201 tattatcaat aatatcacca gatccatgtg cgacccagcg ggtggctggc tccaggtgtg
    19261 cctgactatc gacaatccgc agttcgctaa aacccatcgt tttcattgcc cgcgccgctg
    19321 ccccaatatt ttctgctctg gcgggtgcga ccaggataat cgttatacgc atattgccac
    19381 tcttcttgac caaataaccg cgaacagggt gatcactgtc aacttattac gcggtgcgaa
    19441 tttacaaatt cttaacgtaa gccgcagaaa aagcccttta cttagcttaa aaaaggctaa
    19501 actattttct gactgcacta acggtttaat tgttaaaaaa tgctacatat ccttctgttt
    19561 acttaggata attttataaa aaataaatct cgacaattgg attcaccacg tttattagtt
    19621 gtatgatgca actagttgga ttattaaaat aatgtgacga aagctagcat ttagatacga
    19681 tgatttcatc aaactgttaa cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag
    19741 ttttattggg tgtccggccc ctcttagcct gttatgttgc tgttaaaatg gttaggatga
    19801 cagccgtttt tgacactgtc gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt
    19861 tgttgacgtt gatggaaagt gcatcaagaa cgcaattacg tactttagtc atgttacgcc
    19921 gatcatgtta atttgcagca tgcatcaggc aggtcaggga cttttgtact tcctgtttcg
    19981 atttagttgg caatttaggt agcaaacatg cagaccccgc acattcttat cgttgaagac
    20041 gagttggtaa cacgcaacac gttgaaaagt attttcgaag cggaaggcta tgatgttttc
    20101 gaagcgacag atggcgcgga aatgcatcag atcctctctg aatatgacat caacctggtg
    20161 atcatggata tcaatctgcc gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag
    20221 caggcgaatg ttgcgttgat gttcctgact ggccgtgaca acgaagtcga taaaattctc
    20281 ggcctcgaaa tcggtgcaga tgactacatc accaaaccgt tcaacccgcg tgaactgacg
    20341 attcgtgcac gcaacctgct gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt
    20401 cgtagcgttg aaagctacaa gttcaatggt tgggaactgg acatcaacag ccgttcgttg
    20461 atcggccctg atggcgagca gtacaagctg ccgcgcagcg agttccgcgc catgcttcac
    20521 ttctgtgaaa acccaggcaa aattcagtct cgtgctgaac tgctgaagaa aatgaccggc
    20581 cgtgagctga aaccgcacga ccgtactgta gacgtgacga tccgccgtat tcgtaaacat
    20641 ttcgaatcta cgccggatac gccggaaatc atcgccacca ttcacggtga aggttatcgc
    20701 ttctgcggtg atctggaaga ttaatcggct ttaccaccgt caaaaaaacg gcgcttttta
    20761 gcgccgtttt tatttttcaa ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt
    20821 ctttactggc tttcattttc ggcagtgaaa acgcatacca gtctatatta cgagtcacaa
    20881 acatcatgcc ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg
    20941 cagagttgag cagtccccac atcactccat ccagcaacaa cagcgcgagg gtaaacaaca
    21001 tgctattgcg ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa
    21061 tcagactggc gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga
    21121 gcaaataaaa catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt
    21181 gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg
    21241 cgtacttagt cgcccggtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc
    21301 taaacgcggg gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat
    21361 tattagcaaa ccagctgctt tgccattgcg cctgaaaacc tgactcgcta gcttcccgtt
    21421 tggctggtag aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aaggtcattt
    21481 cgctattacg cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagtgcca
    21541 tattcagctg caggttctgc ttccgccagt ccccttcggg taaagggata tgcacgccct
    21601 gcccgccttg ctctaacccg gtaccgggtt caatggtcag tgccgttccg ttaacttcag
    21661 gcgctttcac cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc
    21721 ctaagatgat atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta
    21781 gcgttaaatc actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt
    21841 tctgattgcc atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc
    21901 gctccacggt tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa
    21961 gcggcccaac gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc
    22021 tacggtaatc ggcacgttcg acaatcactt gccgaatcag cattatcgga atcaacaaca
    22081 gcaatactgc accaaacaag gtggtcattt tccagaacag gggggatttc aacatagtct
    22141 tctcctttgc aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga
    22201 agtgacggtg aagtcgtagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga
    22261 cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt
    22321 tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt
    22381 caggaatacc actaccggta tccagcacct taagcgtgac gtgttcctga tccacttcgg
    22441 cgcttagcgt tatgcaaccg ctctcgggag taaaatcgat ggcgttatcc agtaaattcc
    22501 ccagcgcctg atccagtaac gccggttcag cagcaacatt aacctcggtg ggcataacat
    22561 gcaaagtgat gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata
    22621 atgccgccac atcaacagca gtcagaacga cttcctgacg attctccagt cttgcctggc
    22681 gtagtaacgt ttccaccagc gcctgcattc gcgcattttg tgtcagaatg ttgtcagtaa
    22741 aacgagccac tacttcaggc ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg
    22801 ccgccagtgg gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt
    22861 tcccttccag cttcacgcgc atactttcca gcgcctgcgc gagtttacgc agctcgctac
    22921 taccgagatc gggaagagga acgggcttat tgtcagtgac ggaatcagca tagcgagtga
    22981 gccgggcaat ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc
    23041 ccaacaaaat ggcgctggcc cataaaattc gccgctcgct gcgtttaatg accggagcca
    23101 tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag ccgcgagccg cccataatcg
    23161 gcgcggcaac atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg
    23221 caccatactg accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgtccaa
    23281 cggctttatt tgccgaatcg aacaatactt tgccatgcgc atcggtcata tagacatgat
    23341 attcattgcg cactttgtta atgccaccaa tattggcgcg aaacgggcga tgttgtagct
    23401 gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg
    23461 ccagctccgc aagtaacgtt gccgtgtcga tcaacgtccc ctccgttgct cttcgcacgc
    23521 ccggtttaac ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta
    23581 aaaaatagcc cagcagcaac cgcatgccga tacgcattac aagcccctca ggctatatcc
    23641 catgccgcga tgagtattaa tcggtgaaag atcggggttg atggcgcgca gcttggcacg
    23701 cagcgtttta atgtggatat cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac
    23761 gctatccatt agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag
    23821 caataaaaac tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc
    23881 gggttcattc aattcaaaat gaccaatacg gatgacggga gacggcgtcg agaacttctt
    23941 cacccgacgc agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc
    24001 cacatagtcg tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc
    24061 cgtcaggaac agtacaggta acgccggata gagcgccagt aactggcggc acaattcaaa
    24121 gccactgata tcaggcaaac caacgtcgag aatcatgacg tcgggaacct gctggcgagc
    24181 tttatccagc accggcaaac ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa
    24241 catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg
    24301 ttgcataaat cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt
    24361 tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt
    24421 ggcgtcataa aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa
    24481 tactacctcg ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac
    24541 ttgctgacaa gaaatggccg catcggaggt atcttctgcc aggcccaatc cccctttaat
    24601 accaccggtt ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa
    24661 ggcttccaca acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc
    24721 aatctcttct gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg
    24781 cttgtatttc atattgttac catttttatc tattacacga gcaattagtt cgtcacggta
    24841 aataacatat tgattaagaa caaaatctca attcttttat ttccgtcaag ccctaaaaca
    24901 tactctacta agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta
    24961 cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt
    25021 attcgcgacc ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat
    25081 gtagcggcga aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact
    25141 ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta
    25201 cgcctgactg cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag
    25261 acatttaccc gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct
    25321 cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca
    25381 gagcacaaat ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc
    25441 tgttcgctgg agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat
    25501 tttctcggca acgcgccgac cattccgccg gtgctctacg gcctgaatga aacgcgtccg
    25561 agtcaggata aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag
    25621 gcagatggct atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg
    25681 tttacctatg aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg
    25741 tgcatgccaa tgctcaatct gacgcgccgt aaaggtcagg atattgagcg atactacccg
    25801 gcagaagacg ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct
    25861 atccgccgtt aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct
    25921 gcggtttcaa cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa
    25981 atagaacagt tacgcagacg taagcggcgc tctgcccatg ctggtaatcc gagaatcgta
    26041 ctcaccagac atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt
    26101 aagtcccggc aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg
    26161 ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct
    26221 tcttcggtca gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg
    26281 cgagaatcaa agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg
    26341 gtgcgtccta aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc
    26401 acctgcatcg cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata
    26461 cgtcgctcgg cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt
    26521 tttttccgta tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg
    26581 tctgtgcgac caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct
    26641 tcggcgaagg atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacaac
    26701 cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt
    26761 tacttccaga ggctgatttt tgggtggcga ttgaagctgg cattgatggc gacagcactt
    26821 tcagttgggt ggtgattgaa aacgccagcc agcgcggcga ggcgcgttcg gcaactttgc
    26881 cgttacctgc ggtgattctg gaaaaagttc gtgaaggcga ggcgcttggt cccgtgagtc
    26941 gcgttatacc ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc
    27001 cgggaaactc actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt
    27061 tcacaatgcc gtgtatcagg cattgtagga cggataaggc gttcacgccg catccgacat
    27121 tcggtgcacg atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc
    27181 ttttcagcaa cacctcttcc agccactgac gcagctccac gggcgcggct ttcaggctgt
    27241 tagatccacg cgtaatcgtc gcgatgcccg cgccgagttc attttttaac tcacgctgac
    27301 tcatttcgcc acgcaacagc tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct
    27361 catctggcgt cagcatcagg tttaacaacg gtaaatggag atcgttttgg taggcattct
    27421 taagcaggtc gacaaaacgt aaccactcct ggtgacgctg ttctgccatc gctgctgaat
    27481 agggtgattg ttgggccata atatgtcgcc attgttagcg ggggaagcaa aatgactccc
    27541 ccggttgtac tcgctaaaga gtacgatagc atatcataaa cgtgcggatc agtaacgacg
    27601 tccccattcc gtggcgctca tcaacgtcgg tttatccccc atgaaatagc ggtagtaagc
    27661 atcataagcc agcacgttct tcacataacc gcgcgtctcg gagaacggaa tactctcgac
    27721 aaatgccaca gcgtcgatac gcccggcgct gttgccaagc caggttcgca cccgtccagg
    27781 tccggcgtta taagctgctg aggagaaaat acggttattg ccaaactgct gataaacata
    27841 ttgcaggtaa ctggtgccaa tgttgatgtt cgtttccgga tccagcaatt gcccagggct
    27901 gctataacca ggaatagaga acatcttcac cgtatgggtc gctgtaccag gcataatctg
    27961 catcaggccg ctggccccta ccggtgattt cactttcgga ttccaggcgc tctcctgacg
    28021 ggcaatcgcc atcgcatagc tttgcgggat ctctttccca ctggtgtaac gtttgaaaag
    28081 atcgttgtaa gccagcggga atcgctcttc cagatgatcc caaagcttcc cggcgatcgt
    28141 tgcctgaacg ctaagatccc accattggtt attgaaagca taccgagcca gttgagcctg
    28201 ctctgttttt gacttgctct tcaccagatt ggcccactcg ctacgcgcgg tattatcgag
    28261 attccagtac atcaactcgc gcacgcgcgc catctccggc ccctgagtca gggtgctgtc
    28321 aacattctgc ggcgctttat caatcttcag ctcatactct tcaccgatgc gttgtgctgc
    28381 aaccatcggg tagaaaccac gctgttgtat gagttgatgc aaaatctctt tcgcttcagc
    28441 ttcacgtccg cgttccagca ataaatccgc ctgccagtaa cgccattcat ctttctcttt
    28501 tgcttccatc ggcagacgcg ccagccaggt attcaggccg cgacgatcgc cggtgccaag
    28561 cgccattcgc acacggcgtt caatcaacga cgtggattgc gagcgcataa tggcgtcatc
    28621 gcgccatttc gcctgctcat cggtgacatc attgcccatc aaacgccagg cgacgatatc
    28681 gcgcagctcc tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg acgggatcat
    28741 cagccgtgca ttttcggcat cctgccgcgc cacactggca aacgccaccg ccgccatttg
    28801 acgggtaaaa tcggtcgcgc cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt
    28861 cgccagtgaa atgattgccg aggcgatagt ctggtaatcg gcaggcatct gccctgccag
    28921 cactgttacc aggcctgtgt tacccgcttt catcgccaga cggatacgct ctaaatacgc
    28981 cagcggatct tgtttacctg acgcacgcca gacgctaaat aacttgtcac aggcgttagg
    29041 ctggctcttg ccggttagcc acagctcttt cgccccttgc caggcttctt cactctgccc
    29101 ggtgttccat ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg gcttttccgg
    29161 gctaaaggct aacaagccac gccagtcttc acgccgcgcc agttcattga cgaaacgaga
    29221 ttgcagcgtg cgagcgggag gaagcgtggg gttagcgcga acaaagttag tgaccgtcac
    29281 cgccggttga ttcatcaggt catcggtgat ctggcggtat tccaggtagg gataaagcgg
    29341 ataatccttc agtccaggca tcatttgttc gaccacatcc atctgtcgat tatcccaggc
    29401 ctgcttgatt tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc gcgccacgct
    29461 gctgaccgtc agcagacaga caccggcagc caacagccgc caggtaactt gtttggcttt
    29521 ttccacaagc gcatcctcta agtgtaaata catcaatgca gcgtcatgcc gcgtaataga
    29581 tttatgctaa ccagtcattg ccgtttacgc cacgttacgg acactttttt acttttactg
    29641 cgaaggtgat cgaccaactg ggattaggcg gggaaacagg ctacacttcg cctttgaaaa
    29701 ctcatcatca cgataaacat agaggcgaag tccaacgtgg ctcaattcgt ttataccatg
    29761 catcgtgtcg gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt
    29821 ttcttccctg gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa gtccaccctg
    29881 ctgcgcatta tggcgggcat tgataaagac atcgaaggtg aagcgcgtcc gcagccagac
    29941 atcaagattg gttatctgcc gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag
    30001 tccattgaag aagcggtttc agaagtggtt aacgccctga aacgcctgga tgaagtgtat
    30061 gcgctgtacg ccgatccgga tgccgatttt gacaagctgg ccgctgaaca aggccgtctg
    30121 gaagagatca ttcaggctca cgacggtcat aacctgaacg tacagctgga gcgtgcggcg
    30181 gatgcgctgc gtctgccaga ttgggacgcg aaaatcgcta acctctccgg tggtgagcgt
    30241 cgtcgcgtgg cgttgtgccg cctgctgctg gaaaaaccag acatgctgct gctcgacgaa
    30301 ccgaccaacc acctggatgc cgaatccgtg gcgtggctgg aacgcttcct gcacgacttc
    30361 gaaggcaccg tggtggcgat tactcacgac cgttacttcc tcgataacgt tgcaggctgg
    30421 atcctcgaac tggaccgcgg tgaaggaatt ccgtgggaag gtaactactc ctcctggctg
    30481 gagcagaaag atcagcgtct ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg
    30541 atcgagaaag agctggagtg ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa
    30601 gcacgtctgg cacgctttga agagctgaac agcaccgaat accagaaacg taacgaaacc
    30661 aacgaactgt ttattccacc tggaccgcgt ctgggcgata aagtgctgga agtcagcaac
    30721 ctgcgtaaat cctacggcga tcgcctgctg attgatgacc tgagcttctc gatcccgaaa
    30781 ggggcgatcg tcgggatcat cggtccgaac ggcgcgggta aatcgaccct gttccgtatg
    30841 atctccggtc aggaacagcc ggacagcggc accatcactt tgggtgaaac ggtgaaactg
    30901 gcatcggttg atcagttccg tgactcaatg gataacagca aaaccgtttg ggaagaagtt
    30961 tccggcgggc tggatattat gaagatcggc aacaccgaga tgccaagccg cgcctacgtt
    31021 ggccgcttta actttaaagg ggttgatcag ggtaaacgtg ttggcgaact ttccggtggt
    31081 gagcgcggtc gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc
    31141 gacgaaccaa ccaacgacct ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg
    31201 gagttcccgg gctgtgcgat ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc
    31261 acgcacatcc tggactacca ggatgaaggt aaagttgagt tcttcgaagg taactttact
    31321 gagtacgaag agtacaaaaa acgcacgctg ggcgcagacg cgctggagcc gaagcgtatc
    31381 aagtacaagc gtattgcgaa gtaatgcgta aaatgccgga tgcggcgcga acgccttatc
    31441 cggcctacaa aaccagcaat ttcaatacgt tgtaaaactg taggcctgat aagacgcggt
    31501 aagcgtcgca tcaggcattg agcaccgaat gccggatgcg gcgtgaacac cttttccggt
    31561 ctacaacagc cttagcatcc tcaatttcgt ggcgggtcac ccgtgcaggt tttttcaaac
    31621 cttgtttaat ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa aaactcattt
    31681 tatacctcgc ctttgagcag tttcgtcacg gttatccttg ctcccccatc atctcccgca
    31741 ccagctccac gcagcgcagg aaacggctgt cgtaatcgtc ctcttcaacc cgcacgaatt
    31801 cgatattgtt ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg
    31861 aactgccgag gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca
    31921 ccagatcgaa acggtattca tcaatcagcg cctgcacgaa cggatgctca cgcccttcgt
    31981 actttttgca gaacgcctgg gtagtgacaa aatcggtgtc aataaacgcc actttattgg
    32041 cgtatttcac tgcaaaatca atgtattgtg cgtgacccaa cgcgatttta tcgtagtcgg
    32101 aatactgcaa tgcgatttca tcaccgccga ggtgtgaaaa gacataatcg cggccatatt
    32161 cccacgcact ggtggtgttg aagatattgg caagtttgtt taccagggtg gatttaccgc
    32221 tcgactcgcc gccaaggatc gccacggtac gcacaaaaaa cggcttcact tcggtaggaa
    32281 tatattccca gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg
    32341 tacgtttcgg atcgaccagc accgtctcga tccccagatg ttccatatac tgtggcgcat
    32401 cggcttcttc cgaggtgtag atcaggtccg gctggatccc cttttcagcc ataaactttt
    32461 tgatgccgtt gctccacaca tcccagccat gcggatacgg ctccatgccc tcttcgttga
    32521 aagcatgaat acggatattt ttctgatatt taaaggtttg caatagccag cgcagacgat
    32581 ccggcaccgt gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat
    32641 cgtcaaaacc cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt
    32701 agatatgtcc ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct
    32761 gccggggaaa ttcaagcccc aaaaaacggt gcaatgcctc cagcttttgc gcgctggggc
    32821 ttttaatttt ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag
    32881 ctacctgctg tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg
    32941 acatatctcc ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag
    33001 ttttttaacg ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg
    33061 aacaatcgcc cggcgaaaac cgtgtttcgc cgcttcagaa atccgctcct ggccactggg
    33121 caccgggcgg atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag
    33181 cggtctgtca cgcaggctgg aaaccatcgc cagcagtaac gctaagtcgg cgctggtttc
    33241 ggttaccttc acgccgccga ccacgttcac aaacacatcc tgatcggcca tttgcagacc
    33301 accgtgacgg tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc
    33361 cacgcggcgc ggattcgcca ttatcgagtg atcgaccagc gcctgaatct ccaccagcaa
    33421 cggacgcgtt ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg
    33481 acttaagaaa attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa
    33541 gacgcccagc tcattcaccg cgccgaagcg gtttttatgg ctgcgtaagg tgcgaaaacg
    33601 ggagtcggca tcgccatcca gaagcaccga acagtcgata cagtgttcca gcactttcgg
    33661 gccagccagc gaaccatctt tggttacgtg tccaaccatg acaatcgcca caccgcgcgt
    33721 tttggcgaaa cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga
    33781 tgattgtaca tccgccatat gcatcacctg gatcgagtca attaccatca gcttcggttg
    33841 ctcttcttcg gcaatcaggc agatctgctc gatgctggtt tccgacaaca tattgagatt
    33901 gtcagtcggc aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc
    33961 ggtgacatac agcgttttca tctgctgggc cagtttgcac agcgtttgca gcaacagcgt
    34021 ggatttcccc gcaccagggt taccgccaat cagaatggca cttcctggca ccacgccgcc
    34081 tcctagtacg cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct
    34141 gatatcggag agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg
    34201 ctcgttacgc gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc
    34261 atgacaggca ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac
    34321 aaaggcgcgt tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg
    34381 atgacgggca ggcaattact tctgattcag gctgccagag aggatgcaga atacccccat
    34441 caggtcagcg tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta
    34501 ggcaatcccc agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc
    34561 cacggtctgc gccagcggga tttcatactc ctgcgcgagg cgagtcagag ttttcgcttt
    34621 gtactgcgcg tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc
    34681 cagttcattg gctaccacgg ctgtcaggcg cagcttgtcg cgcaggtatt cagcaaagaa
    34741 agtaaagccg ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag
    34801 ctgcgttaag cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc
    34861 agcgcctttc agcgtcgcca cgcggctgcg caggctggcg gtaaaatcga gttcgccgcg
    34921 catcgcccgt tcggttacct ccgccaccat ctcgcccgtt ccggccagct tggcaatttc
    34981 atcaatacat tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt
    35041 gcgcaggtgc gggattttcc ccagcggggc gacatccagc tgcgcttcgt gggccaggcg
    35101 tgtagcccgt gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca
    35161 ggcggcaaca atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt
    35221 atccagccca cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat
    35281 cacttcatca ccacttaatg aaagaggcag acccggccat aaagagacat cttcaggcag
    35341 gtcgcaccag gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat
    35401 aacgcatgag gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa
    35461 gggtggaaga tggctcgcac aaaactgaaa ttccggctac atcgggcagt gattgtcctg
    35521 ttctgtcttg ccttgttagt ggcactgatg cagggggcgt catggtttag tcaaaaccac
    35581 cagcgacagc gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg
    35641 ctgaacgttg caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc
    35701 ctcgatcagt taacagatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc
    35761 gatcttatcg cacgttctgg cgaaagcgtc gaagtgcgcg atcggctggc gctcgacggt
    35821 aaaaaagcag gcggctattt taaccagcag attgtcgagc caattgcggg taaaaacgga
    35881 ccgctcggct atctgcgcct gacactcgac acccatacgc tcgccaccga agcccaacag
    35941 gtggataaca ccactaacat tttacgcctg atgttgctgc tctcactggc aatcggcgta
    36001 gtgctgaccc gcacgctgct acagggtaaa cgcacccgct ggcagcaatc gcccttcctg
    36061 ttaaccgcca gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt
    36121 actacaagaa aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac
    36181 ccgtggttta acctggcggt ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc
    36241 gttctgtttc tctggcgcaa tgccgacacg gtagtaattg gtcgcgcgca gaacccgtgg
    36301 aaagagtgta atacccggcg gatggaagaa gataacgtcc gcctggcgcg gcgcagtggc
    36361 ggtggcggcg cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag
    36421 ccggagtacg ataaaactat ctccacgtcg attgtgctca atgcgctgaa cgcgctcggc
    36481 gtcagcgccg aagcgtccgg acgtaacgat ctggtggtga aaaccgtcga aggcgaccgc
    36541 aaagtctcag gctcggccta tcgcgaaacc aaagatcgcg gcttccacca cggcaccttg
    36601 ctactcaatg ccgacctcag ccgcctggca aactatctca atccggataa aaagaaactg
    36661 gcggcgaaag gcattacttc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg
    36721 gggctcacca tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc cattatggcg
    36781 agcacgtgga agcggaaatc atctccccgg acaaaacgcc agacttgcca aacttcgccg
    36841 aaacctttgc ccgtcagagt agctgagagt ggaacttcgg tcaggctccg gcattctcgc
    36901 atctgctgga tgaacgcttt agctggggcg gcgtggaact gcatttcgac gttgaaaaag
    36961 gccatatcac ccgcgcccag gtatttaccg acagcctcaa ccccgcgccg ctggaagccc
    37021 tcgccgggcg actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag
    37081 cgctgttggt tgacttcccg gaccaggaaa aagagctacg ggagttgtcg acgtggatag
    37141 cgggggcggt aaggtaactc gctgattaaa atatccaagg aaggaaatat gatcgatctg
    37201 gaaaaccagg aacgggagat tatcaattta atgttcagcc agcgtatttc ctggctagcc
    37261 gccgtgcgta tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg
    37321 attaattctc taaaacagat cgaaaagacg gaacggctta gctcaaacat caaaagcaaa
    37381 atggcagaaa tttatggttg tccaccagag ttgctgatat gcccttcctg gatgacggct
    37441 gaacataaat gaatagattc gtgaatagat acttgaatgt tttttatttt ctatccatat
    37501 gaaaaataat agtaaatcta taaatcagtt gctattgatg aatagatact aaaaagccgg
    37561 agcagtcccc cactccggct taacacgaca atcgcctttc agcgaaacac aattactctt
    37621 tatcgcccag cagaacggat tccagtgcga ttttgatcat gtcgttgaag gtggtctgac
    37681 gctcagcggc agtggtctgc tcgtgagtgc ggatgtggtc agacacggtg cagatggtca
    37741 gggctttcgc gccaaactct gcagccacgc cgtagatacc agccgcttcc atttccacgc
    37801 cgaggatgcc gtatttttcc atcacgtcga acatttcgcc gtccggagag tagaacaggt
    37861 cagcggagaa caggttaccc acgcgagcat caacgcccag tgctttagct gcatctactg
    37921 cgttacgcac catgtcaaag tcagcgatag cggcaaagtc atggtcttta aaacggatgc
    37981 ggttaacttt ggaatcggtg caggccccca taccgataac gacgtcgcgc agttttacgt
    38041 gcggcagaac tgcgccacag gaaccaacgc ggataatttt cttcacgccg aaatcggtga
    38101 tcagttcttt ggtgtagatg gagcaggacg ggatacccat accgtgaccc attacggaaa
    38161 ttttgcggcc tttgtaagta ccagtgaagc ccagcatacc gcgaacgttg ttcacttcac
    38221 gggcatcttc aaggaaagtt tcagcaatat acttcgcacg cagcgggtcg cctggcatca
    38281 aaactacgtc agcgaaatcg cccatttctg cattaatgtg tggggtagcc attgttttat
    38341 cctttttgtg acataacaaa ggcggagtca acgctccgcc caaatccatc agaacatggc
    38401 tttgccatat tccatatcag aagtaccaaa atattttgcc agagtctggc cgatatccgc
    38461 gaaggtttca cggtgaccca gtgagcccgg ttttactttc gggccatata ccagtaccgg
    38521 aatgtgttca cgcgtgtggt cagtaccggt ccaggtcgga tcgcaaccgt ggtcagcggt
    38581 gaggatcagg atgtcgtcat cgcgcagcag agacatcagc tccggcagac ggcggtcgaa
    38641 cagttccaga cccgcagcat aaccggcgac gtcgcggcgg tggccccagg aagagtcgaa
    38701 gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct tctttcatct ctttgatggt
    38761 ggcgtcaaac agcgcgtcca agccagtcgc tttcactttt ttggtgatac cgcagttggc
    38821 gtagatgtct gcaattttac cgacagaaac cacctggccg tgtttttcat caaccagttt
    38881 ctgcagcacg gtcggtgctg gcggctcaac agccaagtcg tgacggttac cggtacgctg
    38941 gaagttacca gctttgtcac cgataaacgg acgagcgata acacgaccga tattgtagcc
    39001 gccgttggtc agctcttcac gggcgatttc gcacagttcg tagagtttat ccagaccgaa
    39061 agtttcttca tggcaggcaa tctggaacac ggagtcagcg gaggtataga aaatcggctt
    39121 gccggttttc atgtgctctt cgcccagttg atccagaatg accgtaccgg aagagtggca
    39181 gttaccgagg taacccggca gattagcgcg ttcgaccagt ttatccagca gctcttgcgg
    39241 gaagctgttt tcgtgatcgg agaaatatcc ccactcaaac agaaccggga caccggcgat
    39301 ttcccagtga ccagacgggg tatctttacc ggatgacatt tcgtgcgccc atgcgtacgc
    39361 gccgataact tcagcgttgc cgtccattcc cgccggaatg aaaccggtag aaccttcgtg
    39421 tgctttcgcc agccccagac gggtcagatt tggcagattg agcggccctt tacgaccatt
    39481 atcagcttcg cctttggcac aagcttctgc gatatgaccc agggtgtcag ccccgacgtc
    39541 accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat gagtccagca ccataataaa
    39601 tgcacgtttc atatgttctc cgtactcagt gcttcaaatt taaatgatca gatcagtata
    39661 ccgttattcg ctgatacggc gatagacagt tggtgtgctt tccggtgcgt tatcggcaag
    39721 tttaattgcc gctttcaccg ctttcgccgc ttcctgccag ctgttttcgt ctttcgcgag
    39781 gataaccgcc agcggacgct gaccgtctac ctggtcgccc agacgcgcca tatcagtaaa
    39841 gccgacgctg taatcgatgg tgtcagatgc ctgacgacga ccgccgccca ttgcaactac
    39901 tgccatcccc agcgcgcggg tatccatttc actgacaaaa ccttcggtat cagcatagac
    39961 tgctttcgtc agcatcgctg tcggcagata cttcgcgtag ttctcaacga agtcggtcgg
    40021 gcctttttgt gccgctacca tacgaccaaa gacttctgcc gctttaccgt tgtccagcac
    40081 cgcctgcaat ttcgcgcgcg cttcggcgtc atctttcgcc agtttgccgg agatcaacat
    40141 ctccacgcac agcgccatcg tgacatcaaa cagacgcggg ttacgatact cacccgtcag
    40201 gaactgcacc gcttcacgaa cttcaaccgc gttacctgca ctggaggcca gtacctgatt
    40261 catgtcggtg agcagcgcgg tggtgcgcac gccagcgccg ttagccacgc caacaatcgc
    40321 ttcggcaagg gcttcagaga gttcgtaggt cggcataaac gcgccgctac ccactttcac
    40381 gtccatcacc agcgcgtcca gaccttccgc cagtttcttc gccaggatcg aggcagtgat
    40441 cagcgggatg gagtccacgg ttgcggtaat atcacgggtc gcgtagaaac gtttatcagc
    40501 cggagccagt gaactggtct gaccgataat cgccacgccg acgtctttaa taatttcgcg
    40561 gaaacggttg tcatccggga aaatgtcgaa gccagggatg gattccagtt tgtcgagcgt
    40621 accgccagta tgaccgaggc cgcgaccgga gatcatcgga atatagccgc cgcaggctgc
    40681 gaccatcggc cccaacatca gcgaagtgac atcgccgacg ccgccggtcg agtgtttatc
    40741 aacaatcggg ccattcagat gcaggctttt ccagtcgaga acggttcctg aatctcgcat
    40801 cgccatggtc agcgagacac gctcaggcat tgtcatatcg tggaagaaaa tggtcatcgc
    40861 gagggcggca atctgccctt cggaaatagt gttgtcgcga ataccgttga taaagaaacg
    40921 aatttcttca tcgctcagcg catgaccatc acgtttttta cgaataattt cttgtgcgag
    40981 aaacaaggta acctcccgag gaaaagagta tgaggcggct tttgccggat gcgtctacgc
    41041 cttatccggc ctacgggtag atacgtctcg taggcctgat aagcgcagcg catcaggcgt
    41101 aaagcatctt acttagtagc tgctggcgct cttaccgtcg ccgtggccca gcgctttcag
    41161 caggcttgcc agcaggctgg aagcaccaaa gcggtagtga cgcgcatcag cccagtcagc
    41221 accgaacagt tcatctgcga tggcgagata tttctgcgca tcttccgcag tacgcacgcc
    41281 gcccgccggt ttgaaaccaa cggttttttc tacgcccata tcacggatca cttccatcat
    41341 gatgcgagcg ctttccggcg tcgcgttcac agccacttta ccggtagagg ttttgatgaa
    41401 gtccgcaccc gctttgatgg agatttcaga cgctttacgg atcagcgctt cgtctttcag
    41461 ttcgccggtt tcgatgatca ctttcagcag tacattcgct gccgcgcaag cctctttaca
    41521 ggctttcacc aggtcaaaac caacctgctc gtcacccgcc atcagcgcgc ggtacgggaa
    41581 caccacgtca acttcatcag caccgtaggc gattgccgca cgggtttctg ccagcgcgat
    41641 ttcgatgtcg tcgttaccgt gtgggaagtt ggttaccgta gcgatacgga tttccggggt
    41701 gccctgctct ttcagagttt tgcgagcaat cgggataaaa cgaggataga tacagatagc
    41761 ggcggtattg ccgaccggag ttttggcctg atgacacaga gcaattactt tctcgtcggt
    41821 gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt gcacgcaggc tgcttgcttt
    41881 cagatcagtc atttcattct cctggcttgt cgccaataaa attcaccttg cgagtttgtt
    41941 agtattctaa catctactcc gcaacacact tcgatacaca tcacaattaa ggaaatctac
    42001 ttacaagttt gcatcactgt aatgcgatct ggttcaaata attcactttc aaatgaatgc
    42061 gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca cgaaacttct tacaatggcg
    42121 catcaccttc ggcataaaag gaaaagaaga tgcccacttc tcatgaaaat gcactgcaac
    42181 aacgttgcca gcaaattgtc accagcccag tgcttagccc ggagcagaag cgccattttc
    42241 tggcactgga agcagaaaac aatctgcctt acccacagct tcctgccgaa gcccgccgcg
    42301 cgctggatga aggtgtaatc tgcgatatgt ttgaaggtca tgcgccgtac aaaccgcgct
    42361 atgtcttacc cgattacgct cgttttctgg cgaacggttc cgaatggctg gagctggaag
    42421 gcgcgaaaga tcttgatgac gcactctctc tgctgaccat tctttaccac cacgtaccgt
    42481 cggtcacatc gatgccggtc tacctggggc aactggatgc gttgttgcaa ccgtatgtta
    42541 gaattctaac acaaaacgag atcgatgttc gaataaaacg tttctggcgt tacctcgaca
    42601 gaaccctgcc agatgccttt atgcacgcca atatcggccc gtctgattcg cccattaccc
    42661 gcgcgatctt acgtgcagac gcgcaggttt caccgaacct gacctttatc tacgatcctg
    42721 aaatcacccc tgatgacctg ctgctggaag tggcgaagaa catctgtgaa tgtagcaaac
    42781 cgcacatcgc caacggtccg gtgcatgata aaattttcac aaaagggggc tacgggattg
    42841 tgagctgtta caactcactg ccgctggcgg gtggtggcag cacgctggta cgccttaacc
    42901 tgaaagccat tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc actctaccgc
    42961 actactgcca gcagcagatc gccatcatcg atgcgcggtg tgaattcctc tatcaacaat
    43021 cacacttctt tgagaatagc ttcctggtga aagaagggct gattaaccct gaacgttttg
    43081 tgccaatgtt tggcatgtat gggctggcgg aagcggttaa cttgctgtgt gagaaagaag
    43141 ggattgccgc gcgctacggt aaagaagccg ccgcaaatga agtgggttat cgcatcagcg
    43201 cgcaactggc ggagtttgtc gccaataccc ccgtgaaata tggctggcaa aaacgcgcca
    43261 tgttacacgc acagtcgggg atcagttccg atatcggcac cacgccgggc gcgcgtttgc
    43321 cgtatggcga tgagccagat ccgatcaccc atctgcaaac tgtcgccccg catcatgctt
    43381 attattattc cggcatcagc gacattctga cgctcgacga aaccatcaaa cgtaacccgc
    43441 aggcactggt acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa tttaccgcca
    43501 atgtcagcgg taacgatctg gttcgcgtta ccggttatat ggtgcgtttg tcggatttag
    43561 aaaaatatcg cgccgaaggt tcacgcacca acaccacctg gctgggcgaa gaagccgcac
    43621 gcaacactcg tattctggaa cgccagccgc gcgtgataag ccatgaacag cagatgcgct
    43681 ttagtcagta agattatccc cttctcctgc gttgacgggc caggcagtcg tctggctctg
    43741 tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca atccgtggac gatgggacgt
    43801 tgcaatgact gtggggagtg cgtgccacag tgtccgcatc aggcgttgca gattgttgac
    43861 ggcaaagtgg tgtggaacgc tgtggtctgc gagcaatgtg atacctgcct gaagatgtgt
    43921 ccgcaacatg ccacaccaat ggcgcaatcc atgagcgtgg acgaagtgct tagccatgtg
    43981 cgcaaagcgg tgctgtttat tgaagggata acggtaagcg gcggcgaagc cacgacccaa
    44041 ctgccgtttg tagtggcgct gtttactgct atcaaaaacg atccgcaact gcgccatctc
    44101 acctgtctgg tggacagtaa cggtatgttg agcgaaaccg gctgggaaaa attactcccg
    44161 gtgtgtgacg gcgcaatgct cgatctcaaa gcatggggta gcgaatgtca tcaacaatta
    44221 accggacgcg ataatcaaca gattaagcgc agcatctatt tgctggcaga gcgcggcaag
    44281 ctggcggaac tgcgtttgct gatgatccca ggccaggtgg attatttgca acacatcgaa
    44341 gaactggcgg cgtttatcaa gggacttggt gatgttccgg ttcgcctgaa cgcgtttcat
    44401 gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga agacgttgag
    44461 ccgttggctg atgcattaaa ggtgcgcggg gtgagccggt tgatatttcc ggcgctctat
    44521 ttgtgacatg ccggatacgg cctacggttc ggcacagact tgtaggcctg ataagacgcg
    44581 tcaagcgtcg catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg
    44641 gcctacggtt cggcacaaac ctgtaggcat gataagacgc gttaagcgtc gcatcaggca
    44701 ttatgcgccg actgccggat gctgcctacg aagggcaaac tacccacgaa cgttaaacac
    44761 cgcatacgtg ttattaagca acacttcggc aatctcatcc gcgggttccg ggcgtaactc
    44821 gcaaagcacg gcaaacacac gggcagcctg ttccgggcgg ttaggctgcc cctgaaaacc
    44881 gttgagcggc atatccggcg catcggtttc cagcaacaac gatgccagcg gtaattttgc
    44941 gatgacatcg cgggttttac tggcgcgtgg ataggtgata gtaccgccta cgccgatttt
    45001 gtagcccagc tgtacaaacc gttcagcctg ttgctggctg ccagaaaatc cgtggacaac
    45061 accagcacgc ggcaaatcgt ggcgtttaag atgcatcgcc agtttgtcgt gcgtgcgccg
    45121 tgaatgcagg atcaccggca gatcgtagcg tttcgccagt ttcagttgtt cgtcgagtaa
    45181 ccactgctgc ctctcaaatt gcggatcgtc gccgaagaga tccagaccga tctcccccac
    45241 cgccaccacc ttcgccggac gcctttccag cgcctgctgt agctgctcaa gagacacatc
    45301 gctatgtttt tccaacatac caggatgcaa gcccaatacg gcatacagcg gttgataatt
    45361 ttccgctaac gcctgcacac gggcaaaatt ttccgcctct gtcgccggaa caatgatctt
    45421 gcctacgccc gcttgtgccg cgcgttgcag gctggcctct tcatcgccaa taaaaggcgg
    45481 gaaatcaaaa tggcagtgag tgtcgataaa acggcaaatc aagccagatc ctcattgtta
    45541 aatgtcgggt cgttcgcctg cggagcgtcg ctcacttcgg caaccagcgt atcgttggcg
    45601 actggcgcag gcggaatgac aatcgattcc ggcgtcacca ctggcaccag atggcgggta
    45661 agcggcgctt tttcagttaa tagcttgcca accgtggcga ggaaataacg accgcataaa
    45721 cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac tgccaagcgc gatactatgt
    45781 aatggcttcg gcggataaat ttcggatatc cgcagcttgc ccggtggttt ctcaataaat
    45841 tgctgaatgt cacgatagct ggtttcatga tgctgcacca gattgaccag cggctgcagg
    45901 ctactgtcac ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta gtacatttgt
    45961 gacggcacag tgcggatgac gaccaacgtt ttagcgccct gccttgccgc ctctttaacc
    46021 ggaatcgcat cactgatccc gccatccagg tagttaatgc cttccagcga cactccgcta
    46081 cgataaaagc caggtatcgc actggaggcg cgaatcacat ccagccagtt ttgtttggtt
    46141 ggtaaaaagt aattcggcgc gtagtcatcc tgacgacagg cgcacatata aaacgatttg
    46201 ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg gcatctggct tgcggtggcc
    46261 tccaccagcc agtcgagatc gataagattt cctccacgga caaagcgcaa tggatcgaaa
    46321 aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg cgtaaccggg ctgattgcag
    46381 ataaacgccg agaggttctg cgccccggca gatgtgccga gataaagatc gaaaggatta
    46441 aactgcgcgc gcataaactc atccagcacg ccagccgtga aaattccacg ctgtccgccg
    46501 ccttcgcaca ccagagctat tcgtccaggc tggaacggcc ttagcgacaa cggcgcaata
    46561 ttaccaagcg ttacaggtat tcgctgcccc acttctgcct tcctgttttt gttgtgaaag
    46621 aaagacaaag taacgcaatt tattattcgc taaaaccgca aaagccagtc cgctggactg
    46681 gctttggata aaattttctt aatggtctga aatctagggt cgttttcggc ccatgaacaa
    46741 actcactagg aacagaataa tcccgacgac aaagacaatt ttagctgcgc ctgcagcggt
    46801 accggccaga ccaccaaacc caagtgcggc ggcgattaac gcgataacca gaaatatgat
    46861 gccccaacga aacataagcg tctcctttac catagttaat gtcaccgctt gatatgagcg
    46921 tgaaaatcac tcaatgcgat atttttagtg tggtgcacat cgcgcctccc gacaaagttc
    46981 gggaggacga attacttagt tttcagatca tttttaacgc ttttcacacc atctaccgct
    47041 ttggcgatac tttcagcacg gtcactttgt gcctgagaat ctacggtacc ggagagctga
    47101 accacgccat cggtggtttc aactttcact ttacgtgaag ggacgatatc gtccgccagc
    47161 agtttggctt tgatttcact ggtggtggcg gtgtcacccg cgtagccctt caccgagcct
    47221 tctttagcgt cgcgaacgtg cagtttgtcg ctgacagagg tcacgccttc aacgcctttc
    47281 gccactttca ctgcctcttc ggcctgggcc tgactttcaa cgaaaccgct cagggtcacg
    47341 actttttgat cggttttgac agagatatcg gtgctcttga tgttgtcatg atccaccagc
    47401 gccgccttca ctttcgcggt gatggcgctg tcatccatga aattaccgac tttattcata
    47461 gagctatcga ctttttgccc tgcgctttca ttggtagtct gcgcgttgtt ttccgcgtag
    47521 gcagagccgg tcgcgacggc agaggtcaac attacagcca gcagagtttt cgaaatcttc
    47581 agtcttgtca tagtcatcga tttattcctg tatgtttgct cgtaatttga gcctagcaac
    47641 acgaggttgc attgctgaat agggagagac ttcaccctct acagaagtca atggtcgcca
    47701 tcacaaaagc gatgggtgat gaataacgac cattacagcc tctgaatcag ttattaatat
    47761 cggtagaatg acaatcgacg gctttagata ctaatatcta cgcattgaac ggtatttaac
    47821 gccatcagaa atgtcatcac tttgttaaat atagatcaca attttgaaac cgctcgggat
    47881 atcagcgaaa acataagcaa aagtgaatgt tttaagaaca ttccgtaagc ggctaataag
    47941 gaagggaaat tgacagggcg cagcggctac cgcgccctgg aggcaagaat taatgctcgc
    48001 gggtctggtg gaactgaacg tccggataac gttcctgtgc caggcgcagg ttgaccatgc
    48061 tggtagcgat gtaagcgagg ttatcgccgc catcaagcgc cagttggctt tcgttcttac
    48121 gcttgaactc ttcgaatttc ttcgcgtctg cacattctac ccagcgggca gtggcaacgt
    48181 tgactgactc atacactgct tcaacgttgt attcgctctt caggcgcgct accaccacat
    48241 caaactgcag cacaccaacc gcaccaacga tcagatcgtt gttggagatt ggacggaaca
    48301 cctgcaccgc gccctcttcg gaaagctgta ccagcccttt gagcagctgt ttttgcttca
    48361 gcggatcttt caggcggata cgacggaaca gttctggtgc gaagttcgga ataccggtga
    48421 acttcatcat ctcaccctgg gtaaaggtgt cgccgatctg aatggtgccg tggttgtgca
    48481 ggccgaggat atcgcccgga tacgcttctt caacgtgcga acggtcaccc gccataaagg
    48541 tcagcgcgtc ggagatcacc acatctttcg cagtgcgcac ctggcgcagt ttcatgcctt
    48601 tttcatattt accggacacc acacgcataa acgccacgcg gtcgcggtgt ttcggatcca
    48661 tgttggcctg aattttaaat acgaagccgg taaatttatc ttcgctcgct tctacggtac
    48721 gggtatcggt ctgacgcggc atcggcgcag gcgcccactc caccaggcca ttcagcatat
    48781 gatcaacgcc gaagttaccc agcgcagtac cgaagaatac cggagtgatt tcgcccgcaa
    48841 ggaacagctc tttgtcgaac tcgttagacg cgcctttaac cagttccagt tcgtcacgca
    48901 gctgctgtgc cagatcttca ccaaccgcag catcgagatc cgggttattc agccctttaa
    48961 caatgcggac ttcctgaatg gtgtgccctt taccgctctg atagagatag gtttcatctt
    49021 tataaaggtg gtaaacgcct ttaaacagct tgccgcagcc aattggccaa gtgatcggtg
    49081 cgcagccaat tttcagctcg ttctcaactt catcgagcag ttccatcggg tcgcggatat
    49141 cacggtcaag tttgttcata aaggtgagga tcggcgtgtc gcgcagacgg gtaacttcca
    49201 tcagcttacg ggtacgatct tcaacacctt ttgcggcgtc gataaccatc aggcagcagt
    49261 ccaccgccgt cagggtacga taggtatctt ccgagaagtc ttcgtgcccc ggggtgtcga
    49321 gcaggttaac caggcaatcg tgatacggaa actgcatcac agacgtagta atggagatcc
    49381 cacgctgctt ttccatctcc atccagtccg acttagcgtg ctggttggaa ccacggcctt
    49441 ttactgtacc ggcggtctga atggcctgtc cgaacagcag caccttctcg gtgatggtag
    49501 ttttaccggc gtccgggtga gaaataatgg caaaagtgcg gcgcttcgcc acctctttca
    49561 aataaggaga caacgtcata attctttctt cttgagtaag cggcatcgcg ccgcgcttgt
    49621 tgaaaacgaa aaattgcggc tattttaccc atcaacgggg gggaggcaat cagtgtttac
    49681 acaggagctg ctccagttcg tgcaacgaag aaacggtcca ggtgggcgcg atgccttctg
    49741 gttgctcgcg attgtgcgca ttcagccagc aggtcgcaag cccggcgttg atgccaccga
    49801 gaatatcgga ctcggcagtg tcgccaacca tcagcacgcg tgaacggtca ggattgcccg
    49861 cctgttccag cgcataatcg aaaattttct tattcggttt ggcaacgcca acttcttcgg
    49921 aaatcaccag caaatcgaag taatcacgca ggcccgtccg ttccagacgc acttgctgca
    49981 aggcgctaaa accgttggtg atgatgccga ttttagcgtt gctacgaatg gcgttaagca
    50041 gagaaaccgc gcccggcaac ggcgtacaga tttccgccat cgcattaata aaggcatcgt
    50101 taagcaagcc tgcttcaact ttcagccgtt cggcccagct ctcgaaacgc ccgtgctgaa
    50161 gctgtaatga agtgatcgcg ccgttttgat aatccaccca cagtggcttg ttaacggcct
    50221 gatagtcctg aaaatcttca gcggtaaagg tgacgctgta atcaagaaac atccgctgca
    50281 ggccggtgaa tgagtcaaag gtaaacagcg tttcatcggc atcaaagaaa atccagtccc
    50341 acttcattat tccaccttgt cttacatact gattggcaac gccatgatga tggcgtcttc
    50401 gcgaccgtcc gtggtggggt agtaattgcg gcgaatcgtc gcctcgttaa agcctaaact
    50461 ttcgtacagg gcaatggcgg cagcatttga ggcacggact tccagccata gtgtcgccac
    50521 gccgcgtttt tccagttcgt cgatcagatg ttccagcagc gccctaccca gcccctgacg
    50581 ctgatagtcg ggatcgaccg caatattgaa caatgtagct tcatccagca ccacttgcgt
    50641 aatcgcaaac gccgccattt tgccgttttg cgttaactga aagttgagat aacgctcgcc
    50701 ctggttgctg gcaaaggttt tttcactcca cggaaaggcg tgggcgcgtt gttcaatgtg
    50761 gtaagccgct ggtaaatcag tcgtttcgag ggaagaaatc gtgttcatat gtgcaaattt
    50821 gttgccataa cgcggcgcgt gccgttgggt ttgcccgtaa ttcggtgagc gccggtgatg
    50881 ccacctgagc gccttccaat gatagcggtt cgtcagtacc caaccgccag ctgttgcagc
    50941 gactgccttg cggcagcatc gcgatttttt ctggcgtcag ttgcagcact tgatcggggc
    51001 tgacggttaa tgcgcgcaga acatcgctca ctaaaggatc agtcagggcg ggaagatcgt
    51061 ttgccaccat caccagacgg acgtgtgccg ggctcgcaat ggcaatctcc ccctgcaacg
    51121 cgccagggcg acgcagcgac cactgggtaa tgcccagttg ctgtaactgc cagtctcgtc
    51181 gggatgtcat agcgaatcgc tcctgttgtc aggggcgcaa atatagcaaa ttcgtcgata
    51241 ccgcgccaac atatggctat aatcgccgcc agtatcaatt gaggagcatt ccatgtctgc
    51301 atttaccccg gcaagtgaag tcttgctgcg tcacagtgat gatttcgaac aaagccgtat
    51361 tctgtttgcc ggagacttac aggatgacct gcccgcgcgt ttagataccg cggccagccg
    51421 tgctcatacc cagcaattcc accattggca ggtgttaagc tgccagatgg gggataacgc
    51481 ccgttttagt ctggtcgcca cggcgaatga tgtcgcagat tgcgatacgc tgatttacta
    51541 ctggccgaag aacaaaccgg aagcccagtt ccagttgatg aatttacttt ctctgctgcc
    51601 agtggggaca gatatttttg tcgttggcga gaaccgcagc ggcgtgcgca gcgctgagca
    51661 gatgctggca gattatgcgc cgttgaataa agtcgacagc gctcgtcgct gtggcctcta
    51721 ttttggtcgt ctggaaaaac agccagtatt tgatgcggat aaattctggg gcgaatacag
    51781 cgtcgatggc ctgacggtca aaacgctgcc tggcgtgttt agccgcgacg gtctggatgt
    51841 cggtagccag ttgctgctct cgacgttaac tccgcacacg aaaggtaaag tgctggatgt
    51901 cggctgtggc gcgggtgtac tttcagttgc ctttgcgcgc cattcgccga aaattcgtct
    51961 caccttgtgc gatgtctctg ccccggcggt agaagccagc cgcgcaacac ttgcagccaa
    52021 cggtgttgaa ggtgaagtct ttgccagcaa cgtcttttcc gaggtgaaag gttgttttga
    52081 tatgatcatc tccaacccgc cgttccacga tgggatgcaa accagcctgg atgcggcgca
    52141 aacgctgatt cgtggcgcgg tgcgtcatct taatagcggc ggcgagctgc gaattgtagc
    52201 gaacgccttc ctgccttacc cggacgtgct ggatgagaca tttggcttcc atgaagttat
    52261 cgcgcaaacc gggcgcttca aggtatatcg cgccattatg acccgccagg cgaagaaagg
    52321 ttgagtatct gatatctgtc gccgcatacc gtgtcggatg cggcgtgaac gccttatccg
    52381 acctacaaaa cctgcacgtt agcccttcgt aggccagata agacgcgcca gcgtcgcatc
    52441 tggcatctgc ccccgcatgc ctaccaaacc tgcacgctag tttcctgatg gacatttttc
    52501 cagcaattac acatcaggcg ataattaact attgacgaaa agctgaaaac cactagaatg
    52561 cgcctccgtg gtagcaattc tttttaagaa ttgatgg
//
LOCUS       DAJTSF010000016        49830 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000016 DAJTSF010000000
VERSION     DAJTSF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.016"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1224
                     /locus_tag="OO335_001030"
     CDS             <1..1224
                     /locus_tag="OO335_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6001509.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="OO335_001031"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="OO335_001031"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6001510.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="OO335_001032"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="OO335_001032"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR6001511.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="OO335_001033"
     CDS             2714..3427
                     /locus_tag="OO335_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6001512.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="OO335_001034"
     CDS             complement(3399..4076)
                     /locus_tag="OO335_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6001513.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="OO335_001035"
     CDS             complement(4070..4747)
                     /locus_tag="OO335_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6001514.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5118)
                     /locus_tag="OO335_001036"
     CDS             complement(4735..5118)
                     /locus_tag="OO335_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6001515.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5134..5442)
                     /locus_tag="OO335_001037"
     CDS             complement(5134..5442)
                     /locus_tag="OO335_001037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001516.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5443..6021)
                     /locus_tag="OO335_001038"
     CDS             complement(5443..6021)
                     /locus_tag="OO335_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6001517.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="OO335_001039"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="OO335_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="OO335_001040"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="OO335_001040"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6001518.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAQIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="OO335_001041"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="OO335_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6001519.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="OO335_001042"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="OO335_001042"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6001520.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="OO335_001043"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="OO335_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6001521.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="OO335_001044"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="OO335_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6001522.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="OO335_001045"
     CDS             12291..13031
                     /locus_tag="OO335_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6001523.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="OO335_001046"
     CDS             13198..14148
                     /locus_tag="OO335_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6001524.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="OO335_001047"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="OO335_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="OO335_001048"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="OO335_001048"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6001525.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="OO335_001049"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="OO335_001049"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6001526.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="OO335_001050"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="OO335_001050"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR6001527.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="OO335_001051"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="OO335_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6001528.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="OO335_001052"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="OO335_001052"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6001529.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="OO335_001053"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="OO335_001053"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6001530.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="OO335_001054"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="OO335_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6001531.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="OO335_001055"
     CDS             23768..23827
                     /locus_tag="OO335_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6001532.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="OO335_001056"
     CDS             complement(23928..24530)
                     /locus_tag="OO335_001056"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6001533.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="OO335_001057"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO335_001057"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="OO335_001058"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="OO335_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6001534.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="OO335_001059"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="OO335_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6001535.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="OO335_001060"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="OO335_001060"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6001536.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="OO335_001061"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="OO335_001061"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6001537.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="OO335_001062"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="OO335_001062"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6001538.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29676..29759)
                     /locus_tag="OO335_001063"
     CDS             complement(29676..29759)
                     /locus_tag="OO335_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001539.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30050..31144
                     /gene="gcvT"
                     /locus_tag="OO335_001064"
     CDS             30050..31144
                     /gene="gcvT"
                     /locus_tag="OO335_001064"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6001540.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31168..31557
                     /gene="gcvH"
                     /locus_tag="OO335_001065"
     CDS             31168..31557
                     /gene="gcvH"
                     /locus_tag="OO335_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6001541.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31676..34549
                     /gene="gcvP"
                     /locus_tag="OO335_001066"
     CDS             31676..34549
                     /gene="gcvP"
                     /locus_tag="OO335_001066"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6001542.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34816..35559
                     /locus_tag="OO335_001067"
     CDS             34816..35559
                     /locus_tag="OO335_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6001543.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="OO335_001068"
     CDS             complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="OO335_001068"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR6001544.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37094..37404
                     /locus_tag="OO335_001069"
                     /pseudo
     CDS             37094..37404
                     /locus_tag="OO335_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37568..38227
                     /locus_tag="OO335_001070"
     CDS             37568..38227
                     /locus_tag="OO335_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6001545.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="OO335_001071"
     CDS             complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="OO335_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6001546.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39646..39912
                     /gene="sdhE"
                     /locus_tag="OO335_001072"
     CDS             39646..39912
                     /gene="sdhE"
                     /locus_tag="OO335_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6001547.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39893..40300
                     /locus_tag="OO335_001073"
     CDS             39893..40300
                     /locus_tag="OO335_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR6001548.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="OO335_001074"
     CDS             complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="OO335_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6001549.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40973..41869
                     /gene="xerD"
                     /locus_tag="OO335_001075"
     CDS             40973..41869
                     /gene="xerD"
                     /locus_tag="OO335_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6001550.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41894..42604
                     /gene="dsbC"
                     /locus_tag="OO335_001076"
     CDS             41894..42604
                     /gene="dsbC"
                     /locus_tag="OO335_001076"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6001551.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42610..44343
                     /gene="recJ"
                     /locus_tag="OO335_001077"
     CDS             42610..44343
                     /gene="recJ"
                     /locus_tag="OO335_001077"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6001552.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44434..45532
                     /gene="prfB"
                     /locus_tag="OO335_001078"
     CDS             join(44434..44508,44510..45532)
                     /gene="prfB"
                     /locus_tag="OO335_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6001553.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45542..47059
                     /gene="lysS"
                     /locus_tag="OO335_001079"
     CDS             45542..47059
                     /gene="lysS"
                     /locus_tag="OO335_001079"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6001554.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47102..47650)
                     /gene="idi"
                     /locus_tag="OO335_001080"
     CDS             complement(47102..47650)
                     /gene="idi"
                     /locus_tag="OO335_001080"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6001555.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYCATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47705..47776
                     /locus_tag="OO335_001081"
     CDS             47705..47776
                     /locus_tag="OO335_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001556.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47773..47898)
                     /locus_tag="OO335_001082"
     CDS             complement(47773..47898)
                     /locus_tag="OO335_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001557.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="OO335_001083"
     CDS             complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="OO335_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6001558.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12052 a        12451 c        13406 g        11921 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc agattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaatacca tgattttttg atttagtgtt ttttgacatt
    29761 tttttagctc ttaatattgt cttattcaaa ttgactttct catcacatca tctttgtata
    29821 gaaattggtg tattttttgg ttttttattc tgtcgcgatt tttgcatttt ttaaccataa
    29881 gctaatgtga tgatcaattt taccttatgg ttaacagtct gtttcggtgg taagttcagg
    29941 caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt ttgtggtgat
    30001 aatttcacca tgaaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    30061 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    30121 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    30181 cggaatgttt gatgtgtcgc atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    30241 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    30301 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gatctcatcg tctactactt
    30361 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    30421 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    30481 gattgccgtg caagggccga atgctcaggc aaaagctgcc acactgttta atgatgctca
    30541 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggtgggcg atctgtttat
    30601 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    30661 ggccgatttc tggcgtgcgc tggtggaagc gggcgttaag ccctgcggtc tgggggcgcg
    30721 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    30781 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccgacag atcgtgactt
    30841 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    30901 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    30961 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    31021 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    31081 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    31141 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    31201 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    31261 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    31321 gttagcgcgg gcgatgactg cgcggttgcc gaatcggtaa aagcggcgtc agacatttat
    31381 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc cccggaactg
    31441 gtgaacagcg aaccgtatgc aggcggctgg atctttaaaa tcaaagccag cgatgaaagc
    31501 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga tgagtaacgg
    31561 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    31621 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    31681 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    31741 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggtgca caatcgttaa acgcgctgac
    31801 cggccagatt gtgccgaaag atattcagct tgcgacacca ccgcaggttg gcgcaccggc
    31861 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    31921 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    31981 gctggaaaat ccgggctggt ataccgcgta cactccgtat cagcctgaag tctcccaggg
    32041 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    32101 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    32161 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccaatg tgcatccgca
    32221 aacgctggat gtggtccgta ctcgtgctga aacctttggt tttgaagtga ttgtcgatga
    32281 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    32341 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    32401 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    32461 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    32521 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    32581 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    32641 gcaacatatt cgtcgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    32701 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    32761 ccgcattcac cgtctgaccg atattctggc ggcgggcctg caacaaaaag gtctgaaact
    32821 gcgccatgcg cactatttcg acaccttgtg tgtggaagtg gccgacaaag cgggcgtact
    32881 gacgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg ccgtggggat
    32941 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagtg tgctgctggg
    33001 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    33061 tatccagcct gcgatgctgc gcgacgacga aatcctcacc catccggtgt ttaatcgcta
    33121 tcacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    33181 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    33241 gatcccaatc acttggacgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    33301 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    33361 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    33421 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    33481 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    33541 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    33601 gggcgataac ctctcctgta tcatggtgac ttatccttct acccacggcg tgtatgaaga
    33661 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    33721 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    33781 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    33841 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    33901 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    33961 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    34021 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    34081 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    34141 gaaagaagaa accggcatca gcgagctgga cattgccaag cgcctgatcg actacggttt
    34201 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    34261 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    34321 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    34381 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    34441 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    34501 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa taccagtaat tcactgattc
    34561 gagtattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    34621 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    34681 gcgcgaatat ttaccctcac cccgaccctc tccctgaaag ggcgaggggg aaaagcgtgc
    34741 caacattgaa gattgagcca gtttgttagc aatctcaaag atacgtcaac gaattaattt
    34801 ttctcggaaa aacaaatggc tatagcactt gtgaccggtg gcagtcgcgg catcgggcgg
    34861 gcaactgcat tactgttggc gcaagaaggg tatacggtgg cggttaatta tcagcaaaac
    34921 ctccacgcgg cgcaggaagt gatgaactta ataatgcaag ccggtggcaa agcattcgtg
    34981 ctccaggcgg atatcagcga cgaaaaccag gtcgttgcga tgtttacagc aatcgatcag
    35041 cacgatgaac cgctagcagc gctggtcaat aacgccggga tcttgtttac ccagtgcacc
    35101 gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt ccaccaacgt gacgggatat
    35161 tttctctgct gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg cagtggcggc
    35221 gctatcgtca atgtctcttc ggtggcctca cggttgggtt cgccagggga atatgttgat
    35281 tacgcggcat cgaaaggggc gattgattcg ttaaccaccg gactatcgct ggaagtcgcc
    35341 gcgcagggga tccgcgttaa ctgcgtgcgg ccagggttta tttataccga aatgcacgcc
    35401 agcggcggcg agcctggacg cgtcgatcgc gttaagtcga acatccccat gcagcgtggt
    35461 ggacaggcag aagaggtcgc gcaggccatt gtctggctac taagtgataa agcctcttac
    35521 gtcacgggaa gttttatcga tttggcgggc gggaaataaa acagggaagt tgtctgaccg
    35581 gatgcaacaa gtattgcatc cggtacttca tcgacttaaa gcttctcgcc gttgctggca
    35641 atcacctctt tgtaccagtt aaagctcttc ttacgtgaac gcgacatatc gccagtaccg
    35701 tcgtcatgtt tattcacata gataaagccg tagcgtttgc tgtactgccc ggtagtgaat
    35761 gacacgcagt cgatgcagcc ccacggtgtg tagcccatca gatccacacc atcgtaagtc
    35821 actgcttttt tcatctcttc aatatgggcg cgcaggtagt caatgcggta gtcgtcgttg
    35881 atgctgccat cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac aataaacagc
    35941 ggcctctgat aacgctcata cagttcgcaa agggcatagc gcagacctac tggatcaatc
    36001 tgccagcccc agtcagacgc tttaacatac gggttcggta cgctgccttc aaaaccagag
    36061 atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg tcatgtaata gctgaaacca
    36121 agataatcgc aggtgccttc gcgcagtaca tcgagatcgc cgtcttccat tttgatatta
    36181 aatccgcgac gttcccactc gttcaataca taggacgggt aatagccgca tagctgcaca
    36241 tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga acatcacatc gtccgggtta
    36301 catgagtagg gatagagcgg caccatcgcc agcatacagc cgactttcat ctccgggtta
    36361 atgcgacgcg cggctttcac tgccagggcg ctggcgacaa actggtgatg cagcacctgg
    36421 tacatcgtct cttccgggtt gtcatgctcg gtatacacca cgccggagca gcagtaacca
    36481 aacagcggcg cacgccagtt acgctggttg tttatttcgt tgaaggtcat ccagtatttg
    36541 actttatgct tgtagcgttc gaataccact tccgcgaaac ggacaaagaa atcaacgaat
    36601 ttacggttgg tccagctacc gtattgctgc accagatgca gcggcatttc aaagtgggag
    36661 agggttatca ccggttcgat gttgtatttc agcagttcat cgaacagaga gtcgtaaaac
    36721 ttcagccctt cttcgtttgg ctgagcttca tcaccttttg gaaaaatacg cgtccaggcg
    36781 atggaagtcc gaaaacattt gaagcccatt tcggcaaata gcttgatgtc ttccttgtag
    36841 tgaccataaa aatcaacggc ttcatggttt ggatagtatt ttcctggcaa gacttctttg
    36901 gtgatttcgc gcggcacgcc gtgtgcgcca caggtcagaa cgtcacaaat gctcggtcct
    36961 ttgccgcctt tgttccagcc gccttcgacc tgatgagcgg caacggcgcc accccataag
    37021 aaatctttcg gtaaggtgag ttttttcact atcataggct ccagatcagt caattcttac
    37081 aggaaatagt accatgcagc caaacgacat cacttttttc aacgtttcca ggatgacatt
    37141 ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt
    37201 gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc
    37261 gcaacctcaa ccgtaacgct ggatacactg acggaaaaac atgcagagca ggaaaatatg
    37321 accctgcctg aactgaaaaa ggtcattgct gacatctatc ccggtcagac acaattttat
    37381 atgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttcgcattt
    37441 aatttcatga tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt
    37501 cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctctgga
    37561 gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc
    37621 caacagcgtc agtcacggca ttgggctggt gtttggtatc gttgggctgg tgttgctact
    37681 ggttcaggcg gtggatctca atgccagcgc cacagcgata accagttaca gcctctatgg
    37741 cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg
    37801 ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac
    37861 ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt
    37921 tatctggagc ctagcattgc tggggattct gttcaaactg accatcgcgc accgattcaa
    37981 aattttatct ctggtgactt atctggcgat gggctggctg tcgctggtgg taatttatga
    38041 aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta
    38101 ttcgctcggg gtgattttct acgtctgcaa acgcattcca tacaaccatg ccatctggca
    38161 cggcttcgtg ctcggcggta gtgtgtgcca ctttctggcg atctatttgt atattgggca
    38221 ggcgtaatgg gtatgccttt gtaggcctga taagacgcgt cagcgtcgca tcaggcatcg
    38281 gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt
    38341 gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa
    38401 atgcctgatg cgatacagag atttactctt cgagcgaata cggcagcggc tcgatatgca
    38461 gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg ttattcatga
    38521 cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac
    38581 gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg
    38641 cactacctgc cagcaaccag agcgcgcgtt tgttggcacc acggaatttt gctcgcgcca
    38701 ccatctcttg tccggtataa cagcctttct taaagctgat accgcccagc gcctggagat
    38761 tggtcgcctg tgggataaac tgcccgctgt tggcggcatc aatcaccggg aaaccagctt
    38821 caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat
    38881 cggtcagcat attagcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt
    38941 caaaccatag caaagtggtc gcgccttctt tgactacctg tttttctttc gaaggcagtt
    39001 cgctaaagag atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac
    39061 gctcgtcgtc tggcgcgatg gtcactttag agaataccgc atattttttc agttcagtca
    39121 gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg tcgcggaaca
    39181 gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat
    39241 cttctgccat ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg
    39301 cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg
    39361 cagaagccgt aggctgacgt ggaggaaaag gtgtaaaagc cataatagag tcctgagttg
    39421 cttaacggcg tattaatgac taatggtaaa agagctatga ggcaatgcaa gtgctttcac
    39481 tacgcgatct gtgccttctg cgaggcgact gccaaaaaaa tgaagccctt gaattgttgt
    39541 gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa gtcgaaaaac
    39601 acgttacaat agccgggtac tggattttcg tgagacagga agaagatgga cattaacaac
    39661 aaagcccgaa ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg
    39721 tcgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt
    39781 ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc
    39841 gatgcagaac tggaaataat ggtccgactc atccagacac ggaaccggga acgtggtcct
    39901 gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg
    39961 gctggttgcc gctgttattt tactcatgcc ctggccgctc agttacaccc cgttatggat
    40021 ggtgttactt tcgctggtgg tatttgattg cgttcgcagc cagcggtgta ttaatgctcg
    40081 ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag
    40141 catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg
    40201 cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagccg aatggcggga
    40261 tttacggcgg attttgttgc aacaagagac gcaaagataa agatgacggc aaagcatatg
    40321 cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc
    40381 accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac
    40441 ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg
    40501 ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga
    40561 gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaataa
    40621 ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga
    40681 tttcaccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt
    40741 tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga
    40801 ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca
    40861 taacgtaagc ttctcgattc tgctgtggct tttgcagcta gtgtatcaga atcgctttag
    40921 tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca
    40981 ggagctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga
    41041 aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg
    41101 cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct
    41161 ggagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt
    41221 ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc
    41281 gaaattgccc cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca
    41341 ggcgccatta attgatcagc cactggagct acgcgataaa gccatgcttg aagtgttgta
    41401 tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata tcagcctgcg
    41461 tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc cgttagggga
    41521 agaggcggtt tactggctgg aaacctatct ggaacatggg cgtccgtggc tgctgaatgg
    41581 tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt
    41641 ctggcaccgc attaaacatt atgccgtgct ggcgggtatc gacagcgaaa agctgtcacc
    41701 gcatgtgttg cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt
    41761 ggtgcagatg ctactgggcc acagcgatct ttccaccacg caaatttata cgcatgtcgc
    41821 taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag
    41881 aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg
    41941 ctttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcatcaaaag
    42001 cagcgatatt cagcccgcgc ccgtagctgg catgaagaca gttctgacta acagcggtgt
    42061 gttgtacatc accgatgatg gtaaacatat cattcagggg ccaatgtatg acgttagtgg
    42121 cacggctccg gtcaatgtca ccaataagat gctgttaaag cagttgaatg cgcttgaaaa
    42181 agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat
    42241 tacctgtggt tactgccaca aactgcatga gcaaatggca gactataacg cgctggggat
    42301 caccgtgcgt tatcttgctt tcccgcgcca ggggctggac agcgatgcag agaaagaaat
    42361 gaaagctatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggcaggtaa
    42421 aagcgtcgca ccagccagct gcgacgtgga tattgccgac cattacgcac ttggcgtcca
    42481 gcttggcgtt agcggtactc cggcagttgt gctgagcaat ggcacacttg ttccgggtta
    42541 ccagccgccg aaagacatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa
    42601 ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac
    42661 ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg agtacgcagt
    42721 gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact aagcggtgtc
    42781 gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc
    42841 ggtgatttcg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg
    42901 cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta
    42961 agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat
    43021 aacggtattt cctcccatgc gggagttgag cacgctcgct cgttgggcat cccggttatt
    43081 gttaccgatc accatttgcc gggcgacaca ttacccgcag cggaagcgat cattaaccct
    43141 aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat
    43201 ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgcggcatc
    43261 gcaattccta acctggcaga actgctggat ctggtagcac tggggacagt agcggacgtc
    43321 gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc
    43381 ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg caaatcgcga tgcacaaaaa
    43441 ctagccgcca gcgatttagg ttttgcgctg ggaccgcgtc tcaatgctgc cgggcgactg
    43501 gatgatatgt ccgttggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg
    43561 ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag agatcgaaca aggaatgcag
    43621 gttgaagccc tgaccctgtg cgagaaactg gagcgcagcc gtgacacgct acccggcggg
    43681 ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgtatc
    43741 aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgacgg tacgctgaaa
    43801 ggttccggtc gctccattca ggggctgcat atgcgtgatg cgctggagcg attagacaca
    43861 ctctaccccg gcatgatgct caagtttggc ggtcatgcga tggcggcggg tttgtcgctg
    43921 gaagaggata aattcgaact ctttcaacaa cggtttggcg aactggttac tgagtggctg
    43981 gccccttcgc tattgcaagg cgaagtggtg tcagacggtc cgttaagccc ggccgaaatg
    44041 accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat gttcccggag
    44101 ccgctgtttg acggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg
    44161 aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg atggtattgc ttttaatgtc
    44221 gataccgctc tctggccgga taacggcgtg cgcgaagtgc aactggctta taagctcgat
    44281 atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt
    44341 tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg
    44401 cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat
    44461 cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc
    44521 caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga
    44581 acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac
    44641 cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga
    44701 agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa
    44761 actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgtta
    44821 cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga
    44881 gcgtatgtat ctgcgctggg cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc
    44941 ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta
    45001 cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaaaagcc cgtttgactc
    45061 cggcggtcgt cgccacacgt cgttcagctc tgcgtttgtt tatccggaag ttgatgatga
    45121 tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc
    45181 gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccgaccgg
    45241 gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aacaaagatc aggccatgaa
    45301 gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgccg agaaacaggc
    45361 gatggaagat aacaaatctg acatcggctg gggcagccag attcgttctt atgtccttga
    45421 tgactcccgc attaaagatc tgcgcaccgg ggtagaaacc cgcaacacgc aggccgtgct
    45481 ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa
    45541 catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa
    45601 aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga attgccttcc cgaacgattt
    45661 ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga
    45721 actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat
    45781 gggtaaagcg tctttcgtta ccctgcagga tgtgggtggc cgcattcagc tgtacgttgc
    45841 ccgtgacgat ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg acctcggcga
    45901 catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg
    45961 taccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggataaat tccacggctt
    46021 gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta acgatgaatc
    46081 ccgtaacacc tttaaagtac gctcgcagat cctctctggt attcgccagt tcatggtgaa
    46141 ccgcggcttt atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc
    46201 gcgtccgttt atcacccacc ataacgcgct ggatctcgac atgtacctgc gtatcgcgcc
    46261 ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg
    46321 taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact
    46381 ctacatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac
    46441 tctggcacag gatattctcg gtaagacgga agtgacctac ggcgacgtga cgctggactt
    46501 cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac
    46561 cgacatggcg gatctggata acttcgactc tgcgaaagcg attgctgaat ctatcggtat
    46621 ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc
    46681 agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct
    46741 ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg
    46801 tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcaacgctt
    46861 cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgatgaaga
    46921 ttacgttacc gcactggaac atggcttacc gccgacagca ggtctgggga ttggcatcga
    46981 ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc
    47041 gatgcgtccg gtgaaataag cattacgtta tgctcacaac cccggcaaat gtcggggttt
    47101 tttatttaag ctgggtaaat gcagataatc gtattctggc ttcgcgattt gtcgcctgca
    47161 tcaccatcca cggactgaac gcccacggcg tggcatcaat accgcgtaat acatcagcta
    47221 aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca ctggtggtgc
    47281 gtgcggcaaa taccggacac acttcatttt ccacaatgcc tctcggatcg gtggcgcagt
    47341 agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc
    47401 aacggcggat caccgcgtct tcgctgcttt ctcccagttg tgggtgccca caaaccgagt
    47461 tagtccacac gccaggccac gcttttttgc tcagtgcgcg gcgggtaact aataattgcc
    47521 ccttggcatt aaacagccaa ctggagaacg cgagatgtaa gagggtgtct gccgtgtgtg
    47581 cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt
    47641 ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat aattgaagag
    47701 tgaaatgatt aaccaagaga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca
    47761 ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc
    47821 aacacagtca gaaatgacag gaatagagag agtaaaaagc aacaacagac tgaatatagc
    47881 gcgcattaaa aaattcatat tattctccat gctcattttt cttcagcttg cgcatcagaa
    47941 gccacatacg tactgtcctg acttctaaat ctttatcaga gacggattcc ttcaccaggt
    48001 cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta agggttgcga
    48061 gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa aagaaatcgt
    48121 gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac
    48181 ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga
    48241 acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca
    48301 tttttggcac catgccaaac aggattaata taattcccga agaaatacac acccagcggc
    48361 tatgaacgcg catcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc
    48421 tgttaaacgt gccgcctatc attgtcccta cgccatcgac acgcagcccg caaataatat
    48481 cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact
    48541 cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga tcgaaaatcg
    48601 gcataccaaa cgacatcggg gtgacaatcg caaaccacga ggcatcatgt agcccggata
    48661 aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg
    48721 cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg
    48781 caaaggagat acctaaataa acgggattac catattgcgg attcccttta cctccagcgg
    48841 cccagtcaat acccacctga atgatgctaa gcccaataga agtaataacc acaccggtaa
    48901 ccagtggcgg gaataaaggc atcaagcgac caataagtgg cgctaataat gtggtgataa
    48961 aacccgcggc gatagtggca ccaaatatcc ccagcaggcc gatatccggg ttcatcccaa
    49021 tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc
    49081 ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat
    49141 ccgagctaat gagcatcgca atagcatctt ttgagaggcc cagtcggtca ccaatcataa
    49201 gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca
    49261 gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg
    49321 agggaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagaataaaa
    49381 agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc
    49441 gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg cttaaaccac
    49501 gaaaatgaca agagaaactg acaatggaat tatctgtaat tttctcatat tgatatttca
    49561 ttaatcattc tggcatgtcg cactctcgca tttaatcgtt tttatctgga tagcgctctt
    49621 ttgatcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata atttgaactc
    49681 agtcgcaaaa ttataattaa aacggtcatg ctacctaata tatgcccgtt tattgcatcg
    49741 cgaaatttga gaagacataa ctcattaaat gaaagggatg caaatgaata cgtttatcgc
    49801 tgctgaagct gcggaatgta taggctgtca
//
LOCUS       DAJTSF010000017        49531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000017 DAJTSF010000000
VERSION     DAJTSF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.017"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="OO335_001084"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="OO335_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR6001559.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="OO335_001085"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="OO335_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000487296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6001560.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGV"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="OO335_001086"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="OO335_001086"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6001561.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="OO335_001087"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="OO335_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6001562.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="OO335_001088"
     CDS             5718..6659
                     /locus_tag="OO335_001088"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6001563.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="OO335_001089"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="OO335_001089"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6001564.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="OO335_001090"
     CDS             7679..8929
                     /locus_tag="OO335_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6001565.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="OO335_001091"
                     /pseudo
     CDS             complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="OO335_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="OO335_001092"
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="OO335_001092"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6001566.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10866..12116
                     /locus_tag="OO335_001093"
     CDS             10866..12116
                     /locus_tag="OO335_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6001567.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="OO335_001094"
     CDS             complement(12172..13260)
                     /locus_tag="OO335_001094"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6001568.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="OO335_001095"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="OO335_001095"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR6001569.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="OO335_001096"
     CDS             complement(14194..15243)
                     /locus_tag="OO335_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6001570.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /locus_tag="OO335_001097"
     CDS             15342..16223
                     /locus_tag="OO335_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6001571.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="OO335_001098"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="OO335_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6001572.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="OO335_001099"
                     /pseudo
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="OO335_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20213..21049)
                     /gene="yeiG"
                     /locus_tag="OO335_001100"
                     /pseudo
     CDS             complement(20213..21049)
                     /gene="yeiG"
                     /locus_tag="OO335_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21307..21975
                     /gene="folE"
                     /locus_tag="OO335_001101"
     CDS             21307..21975
                     /gene="folE"
                     /locus_tag="OO335_001101"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6001573.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21992..23068
                     /locus_tag="OO335_001102"
     CDS             21992..23068
                     /locus_tag="OO335_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6001574.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23210..24250
                     /gene="galS"
                     /locus_tag="OO335_001103"
     CDS             23210..24250
                     /gene="galS"
                     /locus_tag="OO335_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR6001575.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24530..25528
                     /gene="mglB"
                     /locus_tag="OO335_001104"
     CDS             24530..25528
                     /gene="mglB"
                     /locus_tag="OO335_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6001576.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25589..27109
                     /gene="mglA"
                     /locus_tag="OO335_001105"
     CDS             25589..27109
                     /gene="mglA"
                     /locus_tag="OO335_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6001577.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27125..28135
                     /gene="mglC"
                     /locus_tag="OO335_001106"
     CDS             27125..28135
                     /gene="mglC"
                     /locus_tag="OO335_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6001578.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28171..29384)
                     /gene="preA"
                     /locus_tag="OO335_001107"
                     /pseudo
     CDS             complement(28171..29384)
                     /gene="preA"
                     /locus_tag="OO335_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29378..30616)
                     /gene="preT"
                     /locus_tag="OO335_001108"
     CDS             complement(29378..30616)
                     /gene="preT"
                     /locus_tag="OO335_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR6001579.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30810..31049)
                     /locus_tag="OO335_001109"
     CDS             complement(30810..31049)
                     /locus_tag="OO335_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6001580.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31052..31771)
                     /gene="sanA"
                     /locus_tag="OO335_001110"
     CDS             complement(31052..31771)
                     /gene="sanA"
                     /locus_tag="OO335_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6001581.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31921..32805)
                     /gene="cdd"
                     /locus_tag="OO335_001111"
     CDS             complement(31921..32805)
                     /gene="cdd"
                     /locus_tag="OO335_001111"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6001582.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32935..33630)
                     /locus_tag="OO335_001112"
     CDS             complement(32935..33630)
                     /locus_tag="OO335_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6001583.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33627..34025)
                     /locus_tag="OO335_001113"
     CDS             complement(33627..34025)
                     /locus_tag="OO335_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6001584.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34264..35211
                     /gene="dusC"
                     /locus_tag="OO335_001114"
     CDS             34264..35211
                     /gene="dusC"
                     /locus_tag="OO335_001114"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6001585.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35582..35665)
                     /gene="yohP"
                     /locus_tag="OO335_001115"
     CDS             complement(35582..35665)
                     /gene="yohP"
                     /locus_tag="OO335_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR6001586.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35889..37325
                     /gene="mdtQ"
                     /locus_tag="OO335_001116"
                     /pseudo
     CDS             35889..37325
                     /gene="mdtQ"
                     /locus_tag="OO335_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37378..38139
                     /locus_tag="OO335_001117"
                     /pseudo
     CDS             37378..38139
                     /locus_tag="OO335_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38256..38834)
                     /locus_tag="OO335_001118"
     CDS             complement(38256..38834)
                     /locus_tag="OO335_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6001587.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39004..39591
                     /locus_tag="OO335_001119"
     CDS             39004..39591
                     /locus_tag="OO335_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6001588.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39765..40697
                     /gene="pbpG"
                     /locus_tag="OO335_001120"
     CDS             39765..40697
                     /gene="pbpG"
                     /locus_tag="OO335_001120"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6001589.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40735..42450)
                     /gene="dld"
                     /locus_tag="OO335_001121"
     CDS             complement(40735..42450)
                     /gene="dld"
                     /locus_tag="OO335_001121"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6001590.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42646..44943
                     /gene="bglX"
                     /locus_tag="OO335_001122"
     CDS             42646..44943
                     /gene="bglX"
                     /locus_tag="OO335_001122"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6001591.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45195..46112
                     /gene="osmF"
                     /locus_tag="OO335_001123"
     CDS             45195..46112
                     /gene="osmF"
                     /locus_tag="OO335_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR6001592.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46119..47276
                     /gene="yehY"
                     /locus_tag="OO335_001124"
     CDS             46119..47276
                     /gene="yehY"
                     /locus_tag="OO335_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR6001593.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47269..48195
                     /gene="yehX"
                     /locus_tag="OO335_001125"
     CDS             47269..48195
                     /gene="yehX"
                     /locus_tag="OO335_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR6001594.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48200..48931
                     /gene="yehW"
                     /locus_tag="OO335_001126"
     CDS             48200..48931
                     /gene="yehW"
                     /locus_tag="OO335_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6001595.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48912..49019)
                     /locus_tag="OO335_001127"
     CDS             complement(48912..49019)
                     /locus_tag="OO335_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6001596.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49079..49531)
                     /locus_tag="OO335_001128"
                     /pseudo
     CDS             complement(49079..49531)
                     /locus_tag="OO335_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11955 a        12203 c        13312 g        12061 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgtataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggcga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gtggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagaac cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa
     6661 aatttcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgacca gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgttct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttatttat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcaaata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggt gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaacatt tggattgata
    18001 attgttatcg tttgcattat cgttacgacg caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctagac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 ggggggggag aaagatactg aacgtcatcg ggcagtttaa ggtactggag tcgtgtcgcc
    21001 agcgttgctg ccagccttca aaacagcggt gctcttcgag catttccatg cgtggctcct
    21061 tgttgtgttg tttgcaattt cttcatcata ccgtttattc aatgtgctgt gagtaacttt
    21121 cacttccgta tttgcataac gatgttttaa catctgctga tgaaaggcag cggcaattac
    21181 aataattatc gctgtgaata ctggattatg tgcgccgcct cacgcacaat aatcaagctg
    21241 taaatcagct taataacttt gcccccacgc agggtggagg cgtcacacct gcaggagaaa
    21301 tcataaatgc catcactcag taaagaagcg gccctggttc atgaagcgtt agttgcgaga
    21361 ggactggaaa caccgctgcg cccgcccgtg catgaaatgg ataacgaaac gcgcaaaagc
    21421 cttattgctg gtcatatgac cgaaatcatg cagctgctga atctcgacct ggctgatgac
    21481 agtttgatgg aaacgccgca tcgcatcgct aaaatgtatg tcgatgaaat tttctccggt
    21541 ctggattacg ccaatttccc gaaaatcacc ctcattgaaa acaaaatgaa ggtcgatgaa
    21601 atggtcaccg tgcgcgatat cactctgacc agtacctgtg aacaccattt tgttaccatc
    21661 gatggcaaag cgacggtggc ttatatcccg aaagattcgg tgatcggtct gtcaaaaatt
    21721 aaccgtatcg tgcagttctt tgcccagcgt ccgcaggtgc aggaacgtct gacgcagcaa
    21781 attcttattg cgctacaaac gctgctgggc accaataacg tggctgtctc gatcgacgcg
    21841 gtgcattact gcgtgaaggc gcgtggcatc cgcgatgcaa ccagtgccac gacaacgacc
    21901 tctcttggtg gattgttcaa atccagtcag aatacgcgcc acgagtttct gcgcgctgtg
    21961 cgccatcaca actgattaaa aggcaggaac catggagcgc aacgtcacgc tcgattttgt
    22021 tcgcggcgtc gccattctgg ggatcctgct attaaacatc agcgcctttg ggctaccaaa
    22081 ggcggcttat ctcaatcccg cctggtacga tgctattacg ccgcaggatg catggacctg
    22141 ggcatttctc gatctcatcg gccaggtgaa attcctcacg ctttttgcgc tgctgtttgg
    22201 tgctggcctg caaatgttgc tgccccgtgg cagacgctgg atccagtcgc ggttaacgct
    22261 gttagtcttg ttgggcttta ttcacggttt attgttctgg gacggcgata ttctgctggc
    22321 ttacgggctg gtggggcttg gcgttttgct gttattgggg ctgatttccg acagccagac
    22381 cagccgcgcc tggacgccgg atgcatcggc tattttgtat gaaaaatact ggaagcttca
    22441 cggcggcgtt gaagcgatca gtaatcgtgc cgatggtgtt ggcaacagtt tactggcact
    22501 gggcgcacag tatggctggc aactggcagg gatgatgctc attggtgcgg cgctgatgcg
    22561 cagcggctgg ctgaaagggc agttcagctt acgtcactat cgtcgtactg gttttgtgct
    22621 ggtggcgatt ggggtgacca ttaaccttcc tgccatcgcc ctgcaatggc agctggactg
    22681 ggcgtatcgc tggtgtgcct tcttacttca aatgccgcgg gaactgagtg cgccgtttca
    22741 ggcgattggc tatgcgtcgc tgttttatgg cttctggccg caattgagcc gctttaagct
    22801 ggtgcttgcg atcgcctgcg tcggacggat ggcgctgacc aactatctat tgcaaacgct
    22861 gatttgtacc acgctttttt accacctcgg tttgtttatg cattttgacc gcctggagct
    22921 gctggcgttt gttattccgg tatggctggc gaatatcctc ttctctgtta tctggctgcg
    22981 ttacttccgc caggggccgg tggaatggct ctggcgtcag ttaactttgc gtgctgccgg
    23041 accggcaata tctaaaacat caagataacg atctggatta cattcgttaa caaaacggct
    23101 gtaaccgttt ccattgctgt gactcgattc acgaagtcct gtattcagtg ctgacaaaat
    23161 agccgccagc aagcagtcat ttactgcaat ctcataacag gtagtgaata tgatcaccat
    23221 tcgtgatgta gcgcgtcagg ctggcgtctc tgtggcaacg gtttcccggg tgctcaataa
    23281 cagcacgctg gtcagtgccg acacgcgtga agcagtaatg aaagccgtga gtgagctgga
    23341 ttatcggcca aacgccaatg cccaggcgct ggcaactcag gttagcgaca ccattggcgt
    23401 ggtggtgatg gacgtttctg atgcgttttt cggcgcgctg gtaaaagcgg tggatctggt
    23461 cgcccagcag catcagaaat acgtgctaat cggcaatagc tatcatgaag cggaaaaaga
    23521 gcgtcacgcc attgaggtgt taattcgcca gcgttgtaat gcgttgattg ttcactcaaa
    23581 agcattgagt gacgatgaac tggcgcaatt tatggataac attcccggta tggtgttaat
    23641 caaccgcgtt gtgccggggt acgcccatcg ttgcgtttgc ctggataatc tcagcggtgc
    23701 ccgaatggcg acgcgcatgt tgctgaataa cggtcatcaa cgtattggtt atctttcttc
    23761 cagccacggc attgaagatg acgccatgcg taaagcaggc tggatgagtg cgttgaaaga
    23821 gcaggatatt attccgccgg aaagctggat tggcactggt acgccggaca tgccgggcgg
    23881 tgaggcggcg atggttgaac tgctggggcg caatctacaa cttaccgctg tatttgctta
    23941 taacgacaat atggccgctg gcgcactgac agcattaaaa gataatggca ttgcgattcc
    24001 gttacatctc tcaatcatcg gtttcgatga tattcccatc gcccgttaca ccgacccgca
    24061 attaacgacc gtgcgttatc ccattgcttc aatggctaaa ttagccaccg aactggcctt
    24121 gcagggggca gcaggcaata ttgatcctcg tgccagccac tgttttatgc cgacgttagt
    24181 gcgtcgccat tctgtcgcaa cgcgccagaa tgcggcggcg atcactaact caacaaatca
    24241 ggcgatgtaa ccgctttcaa tctgtgagtg atttcacagt atcttaacaa tgtgatagct
    24301 atgattgcac cgttttaatg ttgtaacccg tatgtaacag tgaataatca ctttcaccga
    24361 ggtaatagcg tcataacaac aattaaagcc gttttctgga gcgttaccgg gcatggaaga
    24421 acgaatttta aaaagtgagc ttcggcgttc agtaacactt cattaactct actgccccgc
    24481 cgagcattta tctcaagcac taccctgcat aagaaaaacc ggagatacca tgaataagaa
    24541 ggtgttaacc ctgtctgctg tgatggccag catgttattc ggtgccgctg cacacgctgc
    24601 tgatactcgc attggtgtaa caatctataa gtacgacgat aactttatgt ctgtagtgcg
    24661 caaggctatt gagcaagatg cgaaagccgc gccagatgtt cagctgctga tgaatgattc
    24721 tcagaatgac cagtccaagc agaacgatca gatcgacgta ttgctggcga aaggggtgaa
    24781 ggcactggca atcaacctgg ttgacccggc agctgcgggt acggtgattg agaaagcgcg
    24841 tgggcaaaac gtgccggtgg ttttcttcaa caaagaaccg tctcgtaagg cgctggatag
    24901 ctacgacaaa gcctactacg ttggcactga ctccaaagag tccggcatta ttcagggcga
    24961 tttgattgct aaacactggg cggcgaatca gggttgggat ctgaacaaag acggtcagat
    25021 tcagttcgta ctgctgaaag gtgaaccggg ccatccggat gcagaagcac gtaccactta
    25081 cgtgattaaa gaattgaacg ataaaggcat caaaactgaa cagttacagt tagataccgc
    25141 aatgtgggac accgctcagg cgaaagataa gatggacgcc tggctgtctg gcccgaacgc
    25201 caacaaaatc gaagtggtta tcgccaacaa cgatgcgatg gcaatgggcg cggtagaagc
    25261 actgaaagca cacaacaagt ccagcattcc ggtgtttggc gtcgatgcgc tgccagaagc
    25321 gctggcgctg gtgaaatccg gtgcactggc gggcacagta ctgaacgatg ctaacaacca
    25381 ggcgaaagcg acctttgatc tggcgaaaaa cctggccgat ggcaaaggtg cggctgatgg
    25441 caccaactgg aaaatcgaca acaaagtggt ccgcgtacct tatgttggcg tagataaaga
    25501 caacttggct gaattcagca agaaataaga ctgattgggc gcagtcgttt actgcgccct
    25561 tattgtatgg ccaacaaggt aaaattatat ggtcagctca acgactccgt cttccgggga
    25621 atacttgttg gaaatgagcg gtatcaacaa gtcctttcct ggtgttaagg cacttgataa
    25681 cgttaattta aaagtccggc cacattctat ccatgcatta atgggggaaa acggtgcagg
    25741 aaaatcgaca ttattaaaat gcctgtttgg tatttatcaa aaagactccg gcaccatttt
    25801 attccagggt aaagagatcg atttccattc cgccaaagag gcactggaaa atggtatttc
    25861 gatggtacac caggagttaa acctggtatt acaacgttcg gtgatggata acatgtggct
    25921 ggggcgatat cccaccaaag gcatgtttgt cgatcaggac aaaatgtacc gcgaaaccaa
    25981 agcgattttt gatgaactgg atattgatat cgatccgcgt gcgcgcgtcg gcacattatc
    26041 cgtttcgcaa atgcagatga tcgaaatcgc caaagcgttt tcctataacg cgaaaattgt
    26101 gattatggat gaaccgactt cttcgttaac cgaaaaagag gtcaatcatc tgttcactat
    26161 tattcgtaaa ttaaaagagc gcggctgcgg tattgtttat atctcgcata aaatggaaga
    26221 aatcttccag ttatgtgatg aagttaccgt attgcgcgac ggtcagtgga tcgccaccga
    26281 accgctggca ggactgacga tggacaagat catcgccatg atggttgggc gttctcttaa
    26341 tcagcgtttt cctgacaaag aaaacaagcc gggcgaagtc atcctcgagg tgcgtaacct
    26401 aacgtcactg cgccagccgt cgattcgcga tgtctcgttt gatctgcata aaggggagat
    26461 cctcggtatt gccgggctgg tgggggcgaa acgtaccgat attgttgaga cgttatttgg
    26521 tattcgcgag aaatcggctg gcaccattac gttgcacggc aaacagatca ataaccataa
    26581 tgccaacgaa gccataaacc acggatttgc actggtaact gaggagcgcc gctcaacggg
    26641 aatttatgcc tatctggata ttggttttaa ctcgttaatt tccaatattc gcaactacaa
    26701 aaataaagtt ggtttactgg ataactcgcg gatgaaaagc gatacccagt gggtgattga
    26761 ttcgatgcgg gtaaaaacgc cgggtcatcg gacgcaaatt ggttcgctct ccggtggtaa
    26821 tcagcaaaag gtgattattg gtcgctggct actaacgcaa ccagaaatat taatgctcga
    26881 tgaaccgacg cgcggtattg atgtcggggc gaagtttgaa atttatcagt taattgccga
    26941 actggcgaag aaaggcaagg ggattattat tatctcctct gaaatgcctg agttgttagg
    27001 gataacagac cgtattctgg tcatgagcaa tggtctcgtt tccggaattg tcgatacaaa
    27061 aacaacaacg caaaacgaaa ttctgcgtct tgcgtctttg cacctttaag attaggggct
    27121 tcccatgagt gcgttaaata agaaaagttt tcttacttac ctgaaagagg gcggtattta
    27181 cgtcgttctt ttagttttgc tggcgattat tattttccag gacccaacat ttttaagtct
    27241 gttgaactta agtaatattc tcacccagtc atcggtgcgt attattatcg cgctcggtgt
    27301 ggcagggtta attgtcaccc aggggaccga tctttctgct ggtcgtcagg tagggctggc
    27361 ggcagtggtg gctgcgacat tattgcagtc catggataac gccaacaaag tgttcccgga
    27421 aatggcgacg atgccgattg cgctggttat tctgattgtc tgtgccattg gtgcggtgat
    27481 cggtttgatc aacggtctga ttatcgctta tctcaacgtg acgccgttca ttaccacgct
    27541 cggcacgatg atcatcgtct atggcatcaa ctcgctctat tacgactttg tcggggcgtc
    27601 gccaatttct ggttttgaca gtggcttctc tacctttgct cagggctttg tcgcgctggg
    27661 gagtttccgt ctctcttaca tcaccttcta cgcgttgatt gcggtggcgt tcgtctgggt
    27721 gttgtggaac aaaacccgct tcggtaagaa catttttgcc attggcggta acccggaagc
    27781 ggcaaaaata tctggtgtca acgtcggcct gaacctgctg atgatctacg cgttgtctgg
    27841 cgtgttctat gcctttggcg ggatgttaga agccggacgt atcggctctg ccaccaacaa
    27901 cctcggcttt atgtatgagc tggatgctat cgcggcgtgc gtggtaggcg gtgtatcgtt
    27961 cagcggcggt gtggggacgg tgattggcgt ggtgaccggg gtaattattt ttaccgtcat
    28021 caactatggc ctgacgtata tcggcgtaaa cccatactgg cagtacatca tcaaaggggc
    28081 gattattatc ttcgccgtag cgctggattc actgaaatac gcgcgtaaga agtgatgtga
    28141 ttgcggatat tgccggatgc gtctctggtt ttacaacgtt accgggtgtt ctttctcgcc
    28201 tttcttaaac ttcacttccc cgagctcaat acaacccacc gggcagacgt gaccacaaag
    28261 cagacaaccc acacatttct cggtattaca atgcggtgtg cgggttttct cgctccattc
    28321 catcgcctgg tgaccgccgt cgtaacagga aatataacag cgtccacagc caacacattt
    28381 gtcaagattg atacggggat agacaatata actgcggtct aaatcttccg ccgggacaat
    28441 attgttattc gccagaccta ccatctcctg caacgaatca aatccctgat cggcgagata
    28501 atgcgataac ccgctcgcca tatcttctac gatgcgatag ccgtactgca taatgccggt
    28561 ggtgacctgt aacgttgctg cgccgagcaa taaaaactcc gccgcatctt cccaggtttc
    28621 aatgccgccg ataccgctga ttgggaaatc gcgcagttct ggatgggggc gcatttgctg
    28681 aatgaagcgc agggcgatcg gttttaccgc tttaccggaa tatccggaaa tactcgattt
    28741 accgttaacg atcggcatac cgattttctg attaagatcg atattggtga tggatttaac
    28801 ggtgttaatt gccgcaatgc catctgcgcc gccgcgcttc gccgccagcg ccacttcgca
    28861 catatcaccg atattcggcg tcatcttcgc cagcattggc agcgtggaac cccgtttcac
    28921 tgcccgacaa tatttttcta ccagctccgg gctttgcccg acatcgctac ccatcgcatg
    28981 agaagtcatt tgcggacagg agaagttaca ctcgatcata tccgcgccag ctgctcccat
    29041 tgctgctcat tttcccccat gatcgaagcg atcaatactt tgtccgggta atcttccttc
    29101 agccgacgca gggcggtcag attctcttcc aacggatgtt cagcaatctg ctccatattt
    29161 ttgaagccga taaaaccggt atcttctttc accagatgat caaaacgcgg cgagacttcg
    29221 ttggcgataa aaaagccgat cgttttaaac accacaccgc cccaacctgt gtcgtaggct
    29281 ttggcacaca tctcatagca gttgcctacc ggcgaagaag agaggcagaa cgggttggga
    29341 aagttcacgc cgcaaaaagt aatcgaaaga tctttcgtta acatgagcaa gctccctcta
    29401 aatagtgatg aatcgcctcg gcggcttctt tcccggtttt cacggcatag accacggttt
    29461 tgtcaccctc aacaatatcg ccagcagcaa agacttgcgg atcgcggatc tgataatttt
    29521 gtgttttgat ggtgttacgc tgctgctcta actccgcaaa ggcatccagt ctggcatgct
    29581 gaccgacggc gagaataatt ttatctgccg ccatcgtcag ttcgcctgat aaccgtacat
    29641 gcttaaacgt gactttattg ccttccacgg ctactggcgt gaatccatcg atgatcgaaa
    29701 cgcccagctc ccgggcgctg gcaaactctt tttcgcttgc cggaaactca tctaactctt
    29761 cacgtgctac gcaagttacc gcctgacagc cgagaacttt cagcgtgctg gctacgtcca
    29821 tcgcgacatc accgccgccg ataattaatg cgctttgtgg aatgctgata tcgccttgcg
    29881 cctgtcgtac acgttgcagg aagtcgacgg caatctcaac gtcgctatgc tcaaacagcg
    29941 gtagcccgga accgcttgat aaccccacgg tgaccagtac cgcgcggttt tccgctttca
    30001 gttgctccag ggtgagtgtt ttaccgattt cgttattgca cttaatggtc acgcccattt
    30061 tttcaatacg ggcgatctct gcatccagca ctgattgcgg taaccggaat tgcggaatac
    30121 cgttacgcaa ccagccaccg gggtgcgctt ctttctcata aatcgtgacg tcataaccct
    30181 ggtttgtcag tgtcacactg gcctgcaatc cggcaggacc tgcgccaata atcgcgactt
    30241 tgccgagcgt tttagtaccg ggctgataaa tttccatccc ggtttgttgt tcaaaatcag
    30301 taacaaaacg ctgtaagcgg ccgatatcaa tgggagcatc aacaccggca cgggtacaac
    30361 cgctttggca taatttctcc gtcgggcaca ctctggcaca aacggcaccg agggcattat
    30421 tttcgcgaat tgtctcggca gcgcctttaa aattacgaaa gtagattgag cgaataaatt
    30481 tccccggatc ggtctgtgcc gggcaagcct gactacaggg agcgtcgtga cataataaac
    30541 agcgagaggc ttctttaatc gccagtaacg gcgtaaaagc cggagtgagt tcatccagat
    30601 aattttgttg cggcatgtga tcttccttaa tgctatgagt aattatggcg aacgctaaat
    30661 aaatattgcg tgctgatatt aaggctatgc gcgtggggaa atttaagctg tgaaaggatt
    30721 cacaaatagc tgcggttcag aatgttatat ccgcagctta ttgagtgaag taataattat
    30781 tctcaataat taacagaaaa gagaaaatat taacgataga aaataagata aacaatcatg
    30841 ccgatagata aaattccgaa gccaatatat gccaccatat aagcgaagaa gagtccggga
    30901 ttttttgctc gccagacata caccggttcc ggtgcatttt taacccgttt atcaatcgcg
    30961 cctgcgcgcc agcctttcca tgcttcgcgg aaacaaaata tcgggataag gaaaaaaacg
    31021 gcaacgacaa aaaactgctg tacatccata actactttcc ttgtttcttt tgtaattcaa
    31081 gtaactgttc gggtgtaacg gcgggatacc cctgcgcatc ttccggcacc tggtgcatag
    31141 ccggaatagg gaccagcggc cctaaaaaac gcggttcacg tttaaaaata taaaggtcag
    31201 ccagcgcacc gaaacgggcg gcaaattcgc gaatacgtac tgacagcata tctttcggtg
    31261 acggtacggc ataacactga gcctgaatcc ccatatgcag cgcaataaat aatgctcgct
    31321 cacagtggaa acgttgggtg ataataatga aatcattagt atcgaaaact ttgcgtgtac
    31381 gcacgatgga atccagcgta cgaaagcctg cgtaatcgag aacaatatct gatgggtcga
    31441 caccagcagc gattaaatct ttgcgcatgg tcatcggctc gttataactt tgcaatgcgt
    31501 tatcgccgct cagtaataga taatttacct taccgctgtt ataggcatta atcgctcctt
    31561 gaatgcggta gcgataatac tgattaataa cgccggtacg ataatatttt gctgtgccga
    31621 gcaccacgcc gacctggcgg taggggagat cctgcaattc gtcgtagata taaggcgcgg
    31681 ttttccagct catccagcga tcgaggccga gcacagtcaa cagcagcaag ccgatcagga
    31741 ctaacaggct gaggaacacg cgctttaaca tgcaggagac tcgagctaag gaaaatttcc
    31801 atcaggctac tttacccgct ggttttgcgc aagaaacggc aaactgtttg ccgggaattt
    31861 cgacaccagg agagggctgg caagccggac aaggcgttca cgccgcatcc ggcaccaggc
    31921 ttaagcgaga agcacgcggt cgatactgtg acagccgaga gctttcagcg ttgcggaggt
    31981 ggcatcccac tgaatcaacg gcgcatcggc tttttctgcc agaaccgcgc gctggatatc
    32041 cgggtaatca taacccttga gattcaacag aattaacgcc ccctgcaacg gcggcagagt
    32101 cgggttgaat gcggcgtttt cagcgtagct gccgctgaaa atacgaccgt ctttacattc
    32161 cagcgcgaca ccgcttggtg acttactgta aggcatgtgc gaacggtttg ccgctgcaat
    32221 cgccgcctga gaaagcgcat caccagtcag cgcatagccg tgatcctgct cgtccatcag
    32281 cagcgtttta atctccagat ctttcggccc aaaggcatcc ggcagatagt cacgcagcgc
    32341 gtgtgcctcg cggcccggca aatgaatacg cagatccaga ccgctgttca gttcattcat
    32401 aaactgacgg cagtgaccac aaggcgtata gttaacggtg atggctgcaa gcgctttttc
    32461 accactcaac caagcgtggc tgatcgcgct ttgttcggca tgaacggttt gctgcattgt
    32521 tgcaccaata aattccatat tggcaccaaa ataccaggtt ccgctcacac cgcgcgcaat
    32581 tgcgccaaca ttaaaattcg acaatggcgt acgcgcacag gccgccgcca gcggaagtag
    32641 tgcgaatgcc agcgcgtctt cgtccagtcc cgttgcgctc ttcagcgatg agacttgctc
    32701 cccggtcaac aaagcgggga agtacttgtc tgccagaata ggttccagtg cagattgcaa
    32761 gttatccgca agttgggcaa aagcggtttg aaaacgtgga tgcatggcgt tgcctcataa
    32821 tcatgtaatg ggataacagt ttacgaaccc gttatggctt tatatgtgat ctgaatctca
    32881 ttaattatgc aacacgtgaa acttacatca aaaatgcgcg acgtatcgca aattttagcc
    32941 cattactgcc agaataatcg ggaaaaggaa cggtgcgatc agcgaagtaa ttatcccgca
    33001 taacaccagc gctagcgaac taaatgcacc ttcctgataa tccagctcgg cgcagcgcgc
    33061 cgtaccgagg gcgtgcgagg cagtccccat tgccagaccg cgcgcagctt tggtacgaat
    33121 acgcatcgca ttaagcaatg tatggccaaa taccgcgccg aggatgccga cgaaaatcac
    33181 gcaaactgcg ctgattgccg gaataccgcc aatactgccg ccaaccgcca ttgcaatagg
    33241 cgtggtgact gatttcggca ggattgacgc ggcgatttcc ggtgaagcgc ccatcaacaa
    33301 tgccacggaa gtccccgtca ccatcgcaac cacgctgccg ataaaacaga tggtgatgat
    33361 cgatttccag cgcgcgcgga tctggtgtag ctgctcatat aaaggatagg ccagcgcgac
    33421 gaccgccggt tgcagcagat cgtttagcac ttcgctacct ttaaagtaac tgtcgtaaga
    33481 gatgccggtc agcattaaaa aaggaatgat caccaccatt gctaccagca acgggttaag
    33541 caggggaaac ttataccgtg ccgccagttt gcgggcggca aaaaagacaa tcaacgtcag
    33601 cggtaatgac caccagatat tcgccatcat tcttctgatc ctttctgacc tacgacttta
    33661 cgttcaccgt gtaccagttg tgaactccag ctcaccacca gaaaaaccac cagcgtactg
    33721 acagcacagg aaaccactac cgggccaaac tgcgcgcgga gcaaatcaaa atattgcatg
    33781 acgcctacgc caatcggcac aaacaatagc gccatatagc gaatcagtac gtagcacccc
    33841 ggattgaccc atttcgccgg aagaatttgc aaggccagca ggacaaacag gatcagcatc
    33901 ccgatgatgc tgcccggaat ggttaccggt agcagggaag caatgaaaat gcctgcatac
    33961 aggcaggcat aaatcaggac gaaagcgcgt aaatattgcc agataatgtt cagtgtctta
    34021 ctcataatca acaccccaga tgagatggat tcatcataca attaattcgt aaaacgtgct
    34081 accgatcaca ttaagaaatc agcgcttatt ttttcgctac ccggtattac gcaatgagca
    34141 gcgaactcag gcatacatca agcatagaga gggattgcag tttgcagcaa tccctctttg
    34201 cagaccttta atccgctgac cataggcgct aacatagcgc ctcatttttt tgcgggtgat
    34261 gatatgcgtg tgttactggc accgatggag ggagtgcttg actctctggt gcgtgaattg
    34321 ctgaccgaag ttaacgacta cgatctgtgc atcaccgagt ttgtccgcgt ggtggatcaa
    34381 ctgctgccgg taaaagtctt tcatcgcatt tgccctgagc tacaaaacgc cagccggaca
    34441 ccatctggta cgctggtgcg cgtgcagttg ttaggtcagt tcccacaatg gctggcagag
    34501 aacgccgccc gtgcggtgga gttaggtttc tggggcgtgg atctcaattg cggctgcccg
    34561 tcgaaaacgg ttaacggtag cggcggcggg gcgacgttac tcaaagatcc tgaactcatc
    34621 taccaggggg caaaagcgat gcgtgaagct gtaccggcgc atttgcctgt cagcgtgaaa
    34681 gtgcgtctgg gctgggacag cggtgagaag aaatttgaaa tcgccgatgc ggttcaacag
    34741 gctggcgcta cggagctggt ggtgcatggg cggacgaaag agcagggtta ccgcgcggag
    34801 catattgact ggcaggcgat tggcgagatt cgccagcggc tgaatattcc ggtgattgcc
    34861 aacggtgaaa tctgggactg gcagagcgcg caacaatgca tggcgatcag cggctgcgac
    34921 gcagtgatga ttggtcgcgg ggcgctcaat attcccaacc tgagccgggt ggtaaaatat
    34981 aacgaaccgc gaatgccgtg gccggaggtg gttgctttgc tgcaaaaata tacccgtctg
    35041 gaaaagcagg gcgataccgg gttatatcac gttgcgcgga ttaaacagtg gttgagttat
    35101 ttgcgtaaag aatacgatga agcaacggaa ttatttcagc atgttcgggt gttgaataac
    35161 tcccctgata ttgcaagggc tattcaggca attgatatcg agaaactcta aaaaccatag
    35221 tgatgttttt gacaatgtgt tagctcttta tcaataggcc gcacggttgt ttggtggcgt
    35281 caaaatcgcc gaggcgttcc ctgaaggccg gggcagccca catggatgtg ggctgagggc
    35341 gcgttttaca gggatgttac ctcgcgcccg acccggtagc cgtaagggat aagccgaggg
    35401 caccgcgcag cggcgatttt gttcgccaga gcccgggggg gcagggggcg gcggcgattg
    35461 gtcgccccct gcgcgctcct tgcgccagtg attatatgaa gcaatgcaca tgacaggagc
    35521 gcaacaggat gagagactga aatattctcg ttattttcgg atctgatgac attaattaat
    35581 tttaaaatat catcttaaat acgccagtca ccaccagtag cccaatcagg aaaataatca
    35641 atacagccca gagtataatt ttcattggcg ttcctttatt tgtgagtgct gaataagtat
    35701 aggaaaaaac gaagcccttt gccgttatcc ataaacgatt aagataaatc ggaagatatc
    35761 atttacttag tctatatttt tctgggtatg tcgttttatt gctactgatg tattgccacg
    35821 cgaaactaaa aaatatatcg caacacacgg taatctccgc gcaccgtttc ttacctgatt
    35881 attcccccat gaatcgtgat tccttttatc cagccatcgc ctgttttccg ctgttactga
    35941 tgctggccgg gtgtgcgcct atgcatgaaa cccgccaggc gttaagccag caaacgcccg
    36001 ctgcacaagt tgacaccgca ttacccacgg cgctgaaaaa tggttggcca gacagccaat
    36061 ggtggctgga gtatcacgat aatcagctca cttcattaat taacaatgca ctacagaacg
    36121 caccggatat gcaggtggct gaacagcgca tttagctagc tgaagcccag gcgaaggcag
    36181 ttgccactca ggatggtccg cagatcgact tttcggcgga tatggagcgg caaaaaatgt
    36241 cggcagaagg gctaatgggg ccgtttgctc tgaacgatcc ggccgcaggt acgaccggtc
    36301 cgtggtacac caacggaact tttggcttaa cggcgggctg gcatctcgat atctggggaa
    36361 agaatcgggc ggaggttact gcccgcctgg gtacggttaa agcacgggcg gcggaacgcg
    36421 agcaaacccg ccaattgctg gctggcagcg tagcccgcct gtactgggag tggcaaaccc
    36481 aggcggcgtt aaacacggtc ttgcagcaaa tagaaaaaga gcagaacacc attatcgcga
    36541 ccgatcgcca gctatatcag aacgggatta cttcttcagt tgacggtgtg gaaaccgata
    36601 ttaatgccag caaaacccgg cagcagctca acgatgtcgc ggggaaaatg aaaattattg
    36661 aggcacggtt aaatgcactc acaaatcacc agactaagtc attgaagctt aaaccggtcg
    36721 cgttgccgaa agtggcaagc cagcttcctg atgaactggg gtactcctta ctggcccggc
    36781 gggcagattt gcaggcggcg cactggtacg ttgagtcatc gttaagcact attgatgcgg
    36841 caaaagcggc attttatcct gacatcaacc tgatggcctt cctgcaacag gatgcgttgc
    36901 acttaagcga tctgttccgt cattccgcgc agcaaatggg cgttacggca ggcctgacgc
    36961 tacctatttt cgatagtggt cgtcttaacg ccaatctcga tatcgcaaaa gccgaaagca
    37021 acttgtctat cgccagctac aacaaagcgg tggttgaagc agtgaatgac gtggcgcggg
    37081 cagccagtca ggttcagaca ctggcggaga aaaaccagca tcaggcgcaa attgagcgcg
    37141 atgccttgcg tgtggtaggt cttgcgcagg cccgctttaa cgcgggcatc attgctggtt
    37201 cccgcgtcag cgaagccaga atcccggcgc tgcgtgagcg ggccaatggc ctgttattgc
    37261 aagggcagtg gctggatgcc tccattcgac tcaccggtgc gctgggcggg ggttacaaac
    37321 gctgatgata tgcgccttct atacttaacg tttattcagc gttaagtgga gaactcgatg
    37381 gcataggtcg cgataattac cgcttccgat tcggggatcg gcaaagagtg cgcgttatta
    37441 ctggcgcagc aggggtttga tattggcatt acctggcact cagatgaaga aggggcaaaa
    37501 gataccgcgc gtgaggtggt tacccacggc gtacgtgcgg agatcgtgca actggatctc
    37561 ggcaatctgc cagaaggtgc gcaggcgctg gagacactca tccagcgatt tgggcgtatt
    37621 gatgtgctgg tgaataatgc gggtgcaatg accaaagcgc cgtttcttga tatggctttt
    37681 gatgagtggc gcaagatttt taccgttgat gttgatggtg catttttatg ctcgcaaatt
    37741 gcggctcgtc agatggtgaa ataagggcag ggcggtcgca tcatcaacat tacgtcggta
    37801 catgaacata cgccgctgcc ggatgccagc gcctacacag ccgctaaaca tgcgctcggc
    37861 gggttaacca aaacgatggc gctggagttg gtcaggcata agattttggt gaacgcagtc
    37921 gcgcctgggg cgatagccac gccaatgaac agcatggatg atagcgacgt gaagcccgac
    37981 gcggagcctt cgattcctct acggcgtttt ggcgcaacgc atgagattgc cagcctggtg
    38041 gtgtggcttt gttcggaggg cgcaaattac acgacagggc agtcgttgat agtggatggc
    38101 ggctttatgt tggcgaatcc gcagttcaac ccagaatagc gcagtgaggt gcgtggatat
    38161 ttcaatgggt taggccagat aagacaccag cgtcgcatca ggcatggaac acccggtgcc
    38221 ggatgtggag tgaatgctta tctgtggcaa ctgctttacg cctgattatc cggctttttc
    38281 ttcccgcgcc gtttcagcca ccagcgcacg cccaccacca gaaccacgac cagaatcaac
    38341 cagacccagt gcttcaaatg ctggtcgaga ttgtgcaacc acggcgcaat cacctgacca
    38401 ccagcgtaac caatagtggt aaaaatcaac gcccaggcaa atgcgccgag aatattcagc
    38461 ggcagaaaga ttttcggcgg cagctggctg gcaccaatca gcgtcgggcc aatcacccga
    38521 aagccataca taaagcgcgt accaatgaca aacagatacg gatggcgttg gataagtttc
    38581 tgtgcccgct caattttatc ctgatgtttc gagaaacggc gtaacagctt gccgccaaac
    38641 cgccgcccgc acagatagag cacctggtcg ccaatcatgc caccaagcgc cacagaaagt
    38701 accaccagtg ggaactttaa tagcccctga tgtgccgcaa cgcctcccag caaagtcacg
    38761 gtttcacctt ccgccaggct accgataacc agcgcggcat aaccatattg tgagatgagt
    38821 gtattgagat ccataggcca gtttgtctcc ctttggttgc ctgtaagcat acaccctgtg
    38881 aatcattcac gctgaaaaag gacatacatc agacggtgca aattacatgg atgaacaaaa
    38941 aataatcaca agctggctta tacttgaact gtcaggttca tgaactcaca acaagggggc
    39001 ggtatgagcc atgtctgggg actgttttcc catcccgatc gtgaaatgca ggtgattaat
    39061 cgcgaaaacg aaacgatttc tcaccattac acccaccacg ttttgctgat ggcggcgatt
    39121 ccggtgattt gcgccttcat tggcactaca cagattggct ggaattttgg cgatggcact
    39181 atcctgaagt tatcctggtt taccggactg gcgctggctg tcttgtttta cggcgttatg
    39241 ctggctgggg ttgcggtgat ggggcgggtc atctggtgga tggcgcgcaa ttacccgcag
    39301 cgtccgtctc ttgcgcactg catggtcttc gcgggctacg ttgcaactcc gctgttttta
    39361 agcggtctgg tggcgcttta tccactggtc tggttgtgtg cgctggtcgg cacggtggcg
    39421 ctgttttaca ccggctacct gctttacctt ggtattccga gcttcctgaa tatcaacaaa
    39481 gaggaaggcc ttagcttctc cagttcgacg ctggcgattg gcgtgctggt actggaagtg
    39541 ctgctcaccc taaccgttat actatggggc tacggttatc gtttgttcta gaaagttctt
    39601 tgacgttgca ttgctggcgt aaatgtcagc aatgcagcat ttactcacga ttctcctttg
    39661 acgatcgcac tttttgctcg ttatgatggc gtcgctagcc tcagtaaatc cttactccgg
    39721 cggtgcgcaa cccgtgcgcg tgaaccacta tctgaatgct catcatgccg aaatttcgag
    39781 tttctttatt tagcttggcc ctgatgctgg ctgtgccttt tgcaccgcag gccgttgcga
    39841 aaacggcagc cgctaccacc gcttcacaac cggaaattgc ctccggtagc gcgatgattg
    39901 ttgatctgaa taccaacaaa gtgatctatt cgaaccaccc ggatctggtg cgtccgattg
    39961 cgtctatcag caaattaatg accgcgatgg ttgtgctgga tgcacgactg ccgctggatg
    40021 aaaaactaaa agtggatatc agccagacgc cggagatgaa aggggtctat tcgcgcgtac
    40081 gactgaatag cgaaatcagc cgtaaagata tgctgttgct ggcgctgatg tcttcagaaa
    40141 accgcgcggc ggcaagcctt gcgcaccatt atcccggtgg ttacaaagcc tttattaagg
    40201 caatgaatgc gaaagcgaaa tcgctcggaa tgaacaacac gcgctttgtt gaacctaccg
    40261 gattgtcggt gcataacgtt tcaactgccc gtgacttaac caaactgctc attgccagca
    40321 aacaatatcc gttgatcggg cagttaagta ctacccggga agatatggca accttctcta
    40381 acccgacgta tacgctgccg ttccgcaata ctaatcatct ggtgtatcgc gataactgga
    40441 atattcagtt aaccaaaacc ggctttacca atgcggcggg ccattgtctg gtgatgcgta
    40501 cggttatcaa taataaaccg gtggcgctgg tagtgatgga cgcgtttggc aaatataccc
    40561 attttgccga tgccagccgc ctgcgtacct ggattgaaac cggtaaagtg atgcctgtgc
    40621 cggcagcagc gttgagctat aaaaaacaaa aagccgccca aatggcggcg gcggggcaga
    40681 cggcacagaa cgattaagtg aattcggatg gcgatactct gccatccgta atttttactc
    40741 cacttcctgc cagtttttcc gtttactggt tttaccgatc cccggattca tgctattggt
    40801 cggatcattc tcgcgataga acttctgcaa cgtctccggt gctttataca aatgaccgac
    40861 gttatgctcg gcagggtact gcgcgccgcg ctgctgtagc agttccagca tctgttcttt
    40921 taacgcatgc acatccacgc cttttttcac tatgtaatcc tgatggaaga catagcacat
    40981 aaaatggccg taatagagct tgtgcaccag ctggctgtcg atctccggcg gtaaatgctc
    41041 ataccactcg gtgtcgttac gccgcagagc gatatccagt gccagaatgt cttcgacttc
    41101 atcggaatgc accgcctgat aacgaattgc tgcgcccgca gcggcgaaac ggtgtaaaaa
    41161 cgctttgcta ccttcctccg gcgtacagac aaagaaatcg ccctcggcct gtttgaaata
    41221 atccaccagc cacgatttgg cttcaccgac gccatcgcct gccattttta acagcagatg
    41281 atgctcgtat ttatcgcgcc agtttttcat gcgcggcggt aaatggctgg ggaacaggtg
    41341 accgaatttt tgcattgcac ggtcagtaaa atgcggacgg aagaatttca ctttctccag
    41401 catcgcatcg gtgcgtccct tgagattaaa gaagaacggc atcttgtcgg tgcctaactt
    41461 atcaatcatc aggaaggtgt ctttgccgta tttttccgca atatcgtaga tatcccggtg
    41521 catatattcc ccggcaaccg gcagattttc gaagttagcc agaatatgac ggcggatttc
    41581 ggtcagcact tccggctggt tggtgccgat ataaaacacc tgctgatttt tttccgcttc
    41641 gaaggtatca agacgtaccg caaagacggc cagcttcccg gcgcaaccag aagattcaaa
    41701 taaccgatcc ggatcggcgt tataacgtgc gggcgtgtcg gcttcaatat cacgaacgcg
    41761 gtggacatag tcataatcgt gggcgtgacg accatcgtga cgcacatcgt catctttgat
    41821 gcgatcgtca tccagcttgc taaggatctg ctccggcgtt tcgcccagat caatccccag
    41881 atggttcacc agcgtcagtt tgccatcttc atctatacgc gcgaataacg acatttcggt
    41941 atacgccggg ccacgttgca ccagcgagcc gccggagttg ttacaaatac cgccgatgac
    42001 cgatgcgcct atacacgatg atccaatcac cgaatgcggt tcgcgtccca gcggtttgag
    42061 ggctttttcc agcgaataga gcgtggtgcc cggataggcc agcacctgtt cgcccttgcc
    42121 gagaacgtgc agcttgtcga gacgcagggt gctgatgatc acgatatcgc gatcatagtc
    42181 gttaccgttt ggcgtcgatc cttcggtcag gcctgtattg gcagcctgca tcagaataat
    42241 tttgtcggcg gtgacgcagg ctttcagcac ccgccacaat tccagtagtg agccagggaa
    42301 aacgacagcc agcgcgtcgc cctgaccaga acggaagccc ttgcgatagc gggccgtttt
    42361 tgcgggatcg gtgagcaggt gtgaatgacc gaccagacgg gcaagttcat tcaaaaaggc
    42421 tttattatca gttgttgtca tggaagacat tttccactcc ttgtggtggc gaaaaaatat
    42481 cacaaactag aatagcgcct tgtatgacaa ttcagcgagt gaatattttg aaaattcaga
    42541 caataaactg agcattcttt ttctctctcc atcatgctta tggcaaacta cggtttttgc
    42601 accattttcg ctgccacgtc gggcaacaaa ggaagaaaaa tccatatgaa atggctatgt
    42661 tcagtaggaa tcgcggtgag tctggtcctg cagccagcac tggcggatga tttattcggt
    42721 aaccatccat taacgcctga agcgcgggat gcgttcgtca ccgatctgct caagaaaatg
    42781 acggttgatg agaaaattgg tcagctgcgc ttaatcagcg taggcccgga taatccgaaa
    42841 gaggcgatcc gcgagatgat caaagacggt caggttgggg cgattttcaa caccgtaacc
    42901 cgtcaggata tccgcgccat gcaggatcag gtgatggaat taagccgcct gaaaattcct
    42961 cttttctttg cttacgacgt gctgcacggt cagcgcacgg tgttcccgat tagcctcggt
    43021 ctggcctcgt cttttaacct cgatgcggtg aaaacggtcg ggcgtgtctc tgcttatgaa
    43081 gcggcagatg atggcctgaa tatgacctgg gcaccgatgg tcgatgtctc gcgcgatccg
    43141 cgctggggac gtgcttccga aggttttggc gaagatacgt atctcacctc aataatgggt
    43201 aaaaccatgg tggaagcgat gcagggtaaa agcccggcag atcgttactc ggtgatgacc
    43261 agcgtcaaac actttgccgc atacggcgcg gtagaaggcg gtaaagagta caacaccgtc
    43321 gatatgagtc cgcagcgcct gtttaatgat tatatgccgc cgtacaaagc ggggctggac
    43381 gcaggcagcg gcgcggtgat ggtggcgctg aactcgctga acggcacgcc agccacctcc
    43441 gattcctggc tgctgaaaga tgttctgcgc gaccagtggg gctttaaagg catcaccgtt
    43501 tccgatcacg gtgcaatcaa agagctgatt aaacatggca cggcggcaga cccggaagat
    43561 gcggtgcgcg tggcgctgaa atccggaatc aacatgagta tgagcgacga gtactactcg
    43621 aagtatctgc ctgggttgat taaatccggc aaagtgacca tggcagagct ggacgatgct
    43681 gcccgccatg tactgaacgt taaatatgat atggggttgt ttaacgaccc atacagccat
    43741 ttggggccga aagagtctga cccggtggat accaatgccg aaagccgcct gcaccgtaaa
    43801 gaagcgcgtg aagtggcgcg cgaaagcctg gtgttgctga aaaaccgtct cgaaacgtta
    43861 ccgctgaaaa aatcggccac cattgcggtg gttgggccac tggcggacag taaacgtgac
    43921 gtgatgggca gctggtccgc agccggtgtt gccgatcaat ccgtgaccgt actgaccggg
    43981 attaaaaatt ccgtcggtga aaacggtaaa gtgctgtatg ccaaaggggc gaacgttacc
    44041 agtgacaaag gcattatcga tttcctgaat cagtatgaag aagcggtcaa agtcgatccg
    44101 cgttcgccgc aagagatgat tgatgaagcg gtgcagacgg cgaaacaatc tgatgtggtg
    44161 gtggctgtag tcggtgaagc acaggggatg gcgcacgaag cctccagccg gaccgatatc
    44221 actattccgc aaggccaacg tgacttgatt gcggcgctga aagccaccgg taaaccgctg
    44281 gtgctggtgc tgatgaacgg gcgtccgctg gcgctggtga aagaagatca gcaggctgat
    44341 gcgattctgg aaacctggtt tgcggggact gaaggcggta atgcaattgc cgatgtattg
    44401 tttggcgatt acaacccgtc cggcaagctg ccaatttcct tcccgcgttc tgtcgggcag
    44461 atcccggtgt actacagcca tctgaacacc ggtcgtccgt ataatgccga caagccgaac
    44521 aaatatactt cgcgttattt tgatgaagct aacggagcgc tttatccgtt cggctatggt
    44581 ctgagctata ccactttcac cgtctctgat gtgaaacttt ctgcgccgac catgaagcgt
    44641 gacggcaaag tgacggccag cgtgcaggtg acgaacaccg gtaagcgcga gggggccacg
    44701 gtagtgcaga tgtacttgca ggatgtgacg gcttccatga gtcgccctgt gaaacagctg
    44761 aaaggctttg agaaaatcac cctgaaaccg ggcgaaactc agactgtcag cttcccgatc
    44821 gatattgagg cgctgaagtt ctggaatcaa cagatgaaat atgacgccga gcctggcaag
    44881 ttcaatgtat ttatcggcac tgattccgca cgcgttaaga aaggcgagtt tgagttgctg
    44941 taatgcttta gtaagggcgc gacgtttgcg ccctttgcct gatgttcata tcttaacttg
    45001 ctgaattttc gccctttgta ggccggataa ggcgctcgcg ccgcatccgg caggaaacgc
    45061 cggaccgaat atgcctctta agtatttatc tgattctgca actgaatcct tccgctcagg
    45121 ctaactctgt cattatcaac tatgcttttc tcttaattcg ctgaaaacaa cagtaaaaga
    45181 atgaggaaag catcatgcca ctcttaaagc tctgggcagg ttcactggtt atgttggcag
    45241 ccgtgagcct gccgctacag gcggcttccc ccgttaaagt cggttcaaaa atcgataccg
    45301 aaggcgcgct gctcggcaac atcatcttgc aggtactcga aagccacggc gtaccgacgg
    45361 tgaataaagt gcaactcgga acgactcctg tggggcgcgg ggcgattact tccgatgaac
    45421 tggatatcta tccggaatat accggcaatg gcgctttctt ctttaaagat gaaaacgatg
    45481 cagcgtggaa aaatgccggg cagggctacg agaaagtcaa aaagctcgat gcggagcaca
    45541 acaagttaat ctggctgacg cccgcgcctg caaataacac ctggactatc gccgtgcgtc
    45601 aggatgtggc agagaaaaac aaactcactt cgcttgccga cctgagtcgt tatctgcaag
    45661 agggcggcac cttcaaactg gcagcctcgg cagagtttat cgaacgcgcc gatgcgttac
    45721 ccacgtttga aaaagcctat ggctttaagc tcggtcagga tcagttgctg tcactggctg
    45781 gtggcgacac ggcggtgacg attaaagccg ctgcccagca aacctctggc gttaatgccg
    45841 caatggctta cggcactgac ggtccggtcg cggcgctggg gctgcaaacc ttaagcgatc
    45901 cgcaaggcgt gcaacctatc tacgcgcctg caccagtggt gcgtgaatcg gtgttgaagg
    45961 agtatccgca aatggcacag tggctacagc cagtcttcgc cagcctcgat gcaaaaacat
    46021 tgcagcaact gaatgccagc attgcagtgg aaggactgga tgccaaaaaa gtggctgccg
    46081 actacctgaa acaaaaaggg tgggcgaagt aatttcccgt gacttatcta cgtattaatc
    46141 ctgttctggc gctgctgttg ttgctgacgg caatcgcagc ggcgctgccg tttatcagtt
    46201 acgcgcctaa tcgtttagtc tcgggtgaag ggcttcatct ctggcaactg tggccgcaaa
    46261 cgatctggat gctggtgggc gttggttgcg cctggctgac agcctgtttt gtccccgcta
    46321 aaaaaggcag catttttgca ctcattctgg cgcaattcgt cttcgtattg ttggtgtggg
    46381 gagctggaaa ggcggcgacc caactggcgc aaaatggcag tgcgctggcg cgtaccagcc
    46441 tcggcagagg tttctggctg gctgcggcgc tggcattgct ggcctgtagc gatgccatcc
    46501 gccgaatctc cacgcatccg ctgtggcgct ggttgttgca tatgcagatt gccattattc
    46561 cgctgtggtt gctgtactcc ggcacgctta acgatctctc actaatgaaa gaatacgcca
    46621 accgtcagga tgtgtttgac gacgcgctgg cacaacatct gacgttgctg tttggtgcgg
    46681 tgctgcctgc gttagtgatt ggtgtgccgt tgggcatctg gtgctacttt tccactgcgc
    46741 ggcagggggc aattttttct ctgctcaatg tcattcagac cgtgccttcg gtggcgctct
    46801 ttggcctgtt gattgcgccg cttgccgcgc tggttacggc ctttccgtgg ctggggaagc
    46861 tcggcatagc aggaaccgga atgacacccg cactgattgc gctggtgctc tatgccttgc
    46921 tgccgctggt gcgaggcgtg gtagtcggtt tgaaccagat cccgcgcgat gtgctggaga
    46981 gcgccagagc gatggggatg agcggggcgc agcgtttcct gcatgttcag ttaccgctgg
    47041 cgttaccggt atttctgcgc agcctgcggg tggtgatggt gcaaactgta ggtatggcgg
    47101 tgattgcggc gttaatcggc gcaggcggtt ttggtgcgct ggttttccag gggctgctaa
    47161 gcagcgccat tgatttagtg ttgctggggg tgatcccggt aattgttctg gcggtgctta
    47221 ccgacgcgct gttcgatttg cttatcgcac tgctgaaggt gaaacgtaat gattgaattt
    47281 agccatgtca gcaaactgtt cggcgcacaa aaagccgtta acgatctcaa tctcaatttt
    47341 caggaaggga gtttttcggt gctgattggc acatctggct ccggcaaatc caccaccctg
    47401 aaaatgatta accgcctggt ggagcatgac agcggagtga tccgctttgc cggagaagaa
    47461 attcgctcgc tgccagtact ggagttgcgc cgccggatgg gctatgccat tcaatctatt
    47521 ggcctgttcc cccactggag cgtggcgcaa aacatcgcta ccgtgccgca attacaaaaa
    47581 tggtcgcggg cgcggattga cgatcgtatc gacgaattaa tggcgctact ggggctggag
    47641 tcaaatttgc gtgagcgtta tccgcatcag ctttccggtg gtcagcagca acgtgtggga
    47701 gtggcgcgtg cactggctgc cgatccgcaa gtcttactga tggatgaacc ttttggcgca
    47761 ctggacccgg taacgcgcgg cgcgttgcaa caagagatga cgcgcattca ccgtttgctg
    47821 gggcgcacta ttgtgctggt cactcatgat attgatgagg cgctacggct ggcagaacat
    47881 ctggtattga tggatcacgg tgaagtggtg cagcagggga atccgctgac gatgctgact
    47941 cgtccggcga atgattttgt ccgccagttt tttggacgta gtgaactggg ggtgcacctg
    48001 ctttcgttac gtagcgtggc ggattacgtg cgtcgcgaag aacgggcaga aggtgaggca
    48061 ctggcagaag agatgacgct acgcgatgcg ctctccctgt ttgtcgcgcg gggatgcgag
    48121 gtgctgccgg tggtgaacac gcagggccag ccttgcggca cgctgcattt tcaggatctg
    48181 ctggtggagg cgtaagcgta tgaagatgtt gcgcgatccg ctgttctggc tcattgctct
    48241 ttttgtggcg ctgattttct ggctgcctta cagccagccg ctgtttgctg ccttgttccc
    48301 acaactgcca cgacccgttt atcagcaaga aagttttgca gctctggcac tggctcattt
    48361 ctggctggtg ggaatttcga gtttgtttgc ggtgatcatt ggcacaggtg ccggaattgc
    48421 tgttactcgc ccgtggggcg cggaatttcg cccactggtg gaaactattg ccgccgttgg
    48481 acagactttt ccgcctgtcg cagtgctggc gattgccgtt ccggtgatcg gctttggtct
    48541 gcaaccagcg attatcgcct tgatccttta cggtgtgctg cccatcctgc aggtgacact
    48601 tgccgggctg ggagcgattg atcccagcgt gacagaagtt gcgaaaggta tgggaatgag
    48661 tcgaggtcag cgactgcgta aggtcgagct accgctggcg gctccggtga ttctggcggg
    48721 cgtgcgaact tcggtgatta tcaacattgg tacggcgacg atcgcctcaa cggtaggggc
    48781 cagtacgctg ggtacgccca tcatcatcgg gcttagcgga tttaataccg cgtatgtgat
    48841 ccagggggcg ttactggtgg cactggcggc gatcatcgca gaccgcctgt ttgaaaggct
    48901 ggtgcaggcg cttagccagc acgcaaaata aaggtataac ctgcgagcat gacgccacca
    48961 attccgccta acgccataaa caggaacagg gcgatgaccc caattttagc tatgcgcata
    49021 atgcactcct tatgttaacg aaaggattgt acagtaaagc gcatttgtta acgaatcatt
    49081 aaataccgag tgggaaaata tcatggcctt gttcttgcca actggtgagt tgctgctgtt
    49141 gggcggaggt tcgattttca ccgcaccaca ccagcaatgt acggccttcg aatagttcag
    49201 ggcgtagttg attgagcgag tgggcgagcg aggacatcaa tgcgccatcc ttgttgactg
    49261 gcaatccagc cctccagcca cagacgggtg gtatcctgaa tattccagcc aaccaccagc
    49321 gcatctttac cctgtttttt acgtgccgaa gccagacaaa tggcgatgta gttgatcagt
    49381 acgccgtcga ggatcgccag cagcgcctgg agagtcggtt gttggcactg aagccgtcgg
    49441 cgcagaggaa taaacagatg tgtggtgagt gtctgggcgg ggtaatcctg accgcgctct
    49501 ttgatccacg ttcgcaggct atgtagattg c
//
LOCUS       DAJTSF010000018        48321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000018 DAJTSF010000000
VERSION     DAJTSF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.018"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            187..480
                     /locus_tag="OO335_001129"
     CDS             187..480
                     /locus_tag="OO335_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6001597.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OO335_001130"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OO335_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR6001598.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="OO335_001131"
     CDS             complement(1922..2374)
                     /locus_tag="OO335_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6001599.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OO335_001132"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OO335_001132"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6001600.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="OO335_001133"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="OO335_001133"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR6001601.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OO335_001134"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OO335_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6001602.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OO335_001135"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OO335_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6001603.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OO335_001136"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OO335_001136"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6001604.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OO335_001137"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OO335_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6001605.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OO335_001138"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OO335_001138"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6001606.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OO335_001139"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OO335_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6001607.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OO335_001140"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OO335_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6001608.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="OO335_001141"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="OO335_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6001609.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="OO335_001142"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="OO335_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6001610.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="OO335_001143"
     CDS             complement(15085..15582)
                     /locus_tag="OO335_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6001611.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="OO335_001144"
     CDS             complement(15579..15941)
                     /locus_tag="OO335_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6001612.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="OO335_001145"
     CDS             complement(15931..16278)
                     /locus_tag="OO335_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6001613.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="OO335_001146"
     CDS             16387..16800
                     /locus_tag="OO335_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001614.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="OO335_001147"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="OO335_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="OO335_001148"
     CDS             complement(18324..20039)
                     /locus_tag="OO335_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR6001615.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="OO335_001149"
     CDS             20206..21099
                     /locus_tag="OO335_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6001616.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="OO335_001150"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="OO335_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23526)
                     /locus_tag="OO335_001151"
     CDS             complement(21910..23526)
                     /locus_tag="OO335_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6001617.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23821..24495
                     /locus_tag="OO335_001152"
     CDS             23821..24495
                     /locus_tag="OO335_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6001618.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24534..26195)
                     /locus_tag="OO335_001153"
     CDS             complement(24534..26195)
                     /locus_tag="OO335_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6001619.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="OO335_001154"
     CDS             complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="OO335_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6001620.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="OO335_001155"
     CDS             complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="OO335_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6001621.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27338..27410)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27649..27981
                     /locus_tag="OO335_001156"
     CDS             27649..27981
                     /locus_tag="OO335_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6001622.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27983..29215)
                     /locus_tag="OO335_001157"
     CDS             complement(27983..29215)
                     /locus_tag="OO335_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR6001623.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            29316..30416
                     /gene="cbrA"
                     /locus_tag="OO335_001158"
     CDS             29316..30416
                     /gene="cbrA"
                     /locus_tag="OO335_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR6001624.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30377..31714)
                     /locus_tag="OO335_001159"
     CDS             complement(30377..31714)
                     /locus_tag="OO335_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6001625.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="OO335_001160"
                     /pseudo
     CDS             complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="OO335_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="OO335_001161"
     CDS             complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="OO335_001161"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR6001626.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="OO335_001162"
     CDS             complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="OO335_001162"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR6001627.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="OO335_001163"
     CDS             complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="OO335_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR6001628.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35377..36033
                     /locus_tag="OO335_001164"
     CDS             35377..36033
                     /locus_tag="OO335_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001629.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="OO335_001165"
     CDS             complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="OO335_001165"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR6001630.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37005..37403)
                     /locus_tag="OO335_001166"
     CDS             complement(37005..37403)
                     /locus_tag="OO335_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR6001631.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="OO335_001167"
     CDS             complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="OO335_001167"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6001632.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40086..41159)
                     /gene="recF"
                     /locus_tag="OO335_001168"
     CDS             complement(40086..41159)
                     /gene="recF"
                     /locus_tag="OO335_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6001633.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRICADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="OO335_001169"
     CDS             complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="OO335_001169"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6001634.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="OO335_001170"
     CDS             complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="OO335_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6001635.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43964..44044
                     /locus_tag="OO335_001171"
     CDS             43964..44044
                     /locus_tag="OO335_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001636.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44274..44414
                     /gene="rpmH"
                     /locus_tag="OO335_001172"
     CDS             44274..44414
                     /gene="rpmH"
                     /locus_tag="OO335_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6001637.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44431..44790
                     /gene="rnpA"
                     /locus_tag="OO335_001173"
     CDS             44431..44790
                     /gene="rnpA"
                     /locus_tag="OO335_001173"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6001638.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44754..45011
                     /gene="yidD"
                     /locus_tag="OO335_001174"
     CDS             44754..45011
                     /gene="yidD"
                     /locus_tag="OO335_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6001639.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45014..46660
                     /gene="yidC"
                     /locus_tag="OO335_001175"
     CDS             45014..46660
                     /gene="yidC"
                     /locus_tag="OO335_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6001640.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46766..48130
                     /gene="mnmE"
                     /locus_tag="OO335_001176"
     CDS             46766..48130
                     /gene="mnmE"
                     /locus_tag="OO335_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6001641.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11980 a        13065 c        12219 g        11057 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaactt ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagcaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 acggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa gcttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccgcc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttcg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcagaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat ttccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc
    23461 ggcgaaggga aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact
    23521 gagcatttcc acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg
    23581 cagacctgga gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc
    23641 gtgatcgctt tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga
    23701 catctgtgag cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat
    23761 cgattaagca gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag
    23821 atgatctaca aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg
    23881 ctaaacagcc ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg
    23941 actatccgta aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc
    24001 agcggcactt acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg
    24061 gtggaggtgt taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc
    24121 atgcctgcgc ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac
    24181 ttctcccgtc gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg
    24241 ccggtaaaac tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat
    24301 attgaacaag agtgcgggat tttgattggc ggtaattatg aaagcctgat gccggtgctc
    24361 gccgatagac tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc
    24421 acctcgttgt catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat
    24481 gccagcgaat actaggtgga gtaccattta cggcgactcc acccggaaaa aagttaaccg
    24541 atactccaga agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac
    24601 ggatagacag tggcgtaaga gagcgccgcc gcaccactgg ttggatgaag attattagca
    24661 aacgccagcg ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa
    24721 ttcattttgg ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc
    24781 agggcaccat aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc
    24841 ccaccagatt tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcgcg
    24901 aggttggcac ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg
    24961 atcaacgcca taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct
    25021 ggcacaaaga cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc
    25081 agcatctgaa cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca
    25141 attgcggacg gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca
    25201 ctggcgacca gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct
    25261 ttaaagtgca ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc
    25321 tcgacgcgca aatcagtgcc tttcgtagac agcgaggtat cgacctcctg accaatcacc
    25381 agttgcgcat tatgtaaatc cgctggctgc cccaccagat gcagcaaatc gcccagttgg
    25441 ataatggtat ctggcgaagg tacttttagg gtttcttctc gtttcagacg cgagcagata
    25501 attttgtcgc cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc
    25561 tcaacgcgaa tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc
    25621 tgctgagctt ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac
    25681 aaaatcccgc aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc
    25741 atttccattg gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta
    25801 ttggttaccg caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta
    25861 tgcaggatgg cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg
    25921 cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta
    25981 taaacgaaca ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc
    26041 atccccgcct gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata
    26101 cctaatccta tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa
    26161 gccagaatac tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt
    26221 ttaattgcta cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat
    26281 cacgggaaag aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa
    26341 gaaagacaaa aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt
    26401 aacgcgggac gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga
    26461 attaaatcaa tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca
    26521 gccagcgtaa aactcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc
    26581 tcttcttttg gctgctccgg cgtgcctttt acgctcaggc gcgtgccttc cagttgaatc
    26641 tctaaatctt cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg
    26701 cttttctcaa tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg
    26761 gccagtttgt caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata
    26821 gtcatttctc cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc
    26881 cctgacggcg agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt
    26941 tcgatacggc gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa
    27001 ccatttacca ggttagcccc acgaacatga atgttctcag ctaactggaa tttgcgttca
    27061 aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga
    27121 gcacctttca ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa
    27181 ccagccacag cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga
    27241 gggtagccgc cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat
    27301 ccaatagcag aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga
    27361 aatcagcgag aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca
    27421 ggcgcgtcct catcgactac gatgtaaaat gggtctggaa atgaactttt caagccttat
    27481 cagcggactt ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac
    27541 gttaaagtgc gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat
    27601 cgctataact catcaggcca acacccacga tcaacagatg aatttattat gataagaaat
    27661 gtgttgttag cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg
    27721 atgtcccata ccggggggaa agaaggaacg tatcccggca cccgcgccag cgccaccatg
    27781 attggcgacg atgaaaccaa ctggggcact aagtcgctgg cgattctcga tatgccgttt
    27841 accgctgtaa tggacacgct tttactgccg tgggatgtgt tccgcaaaga cagctcggtg
    27901 cgctcgcgcg ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt tattccaccg
    27961 gccagaatgc ctgataactg atttagcgat cagtttcggt gatccacagc tggccacctt
    28021 ccatccacgc cagccattga ccatccggcg agaagaccac ggcgtccgca gaaggtggat
    28081 tggcgtggtt ttcggttaaa tactcaacct cgccgctttg cgcatgggca caaacaattc
    28141 gattatccag cacaaagccc aaccattctc ctgacggatg ccagttaaat gcagactgaa
    28201 tatccgtttt gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca
    28261 gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac
    28321 agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg
    28381 ttaaacgtcg ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta
    28441 acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata
    28501 gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat
    28561 ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg
    28621 gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct
    28681 tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg
    28741 gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc
    28801 taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat
    28861 ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga
    28921 agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg
    28981 acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct
    29041 cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag
    29101 aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtatttgtat
    29161 tggtattggt attggtgagc aggtgattac ggggagcaaa ggttatctgt ttcatggcgc
    29221 ggtcctgata caaaacggtt cccgccatca tacttcacaa ggacttcact ttcagacaat
    29281 agtcttttgc catcgtgatg aatgtgagtg aaaagatgga acatttcgac gtggcgatta
    29341 ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt agccggcaaa atgcaggtga
    29401 tcgcgctgga taaaaagcac cagtgcggta ctgaaggttt cagcaaacct tgcggcggtc
    29461 tgctggcacc ggacgcgcag cgatctttta ttcgcgatgg actgacgctt cctgtcgatg
    29521 tgatcgccaa tccacagatt ttcagcgtca aaactgtcga cgtcgccgca tcgctcacgc
    29581 gtaactacca gcgaagctat atcaatatta atcgccatgc tttcgacttg tggatgaaat
    29641 cgctgatccc cgccagcgtt gaggtttatc acgatagcct gtgccgaaaa atctggcgtg
    29701 aggatgataa atggcatgtc atttttcgtg cagacggctg ggagcaacat attaccgccc
    29761 gctatctggt cggtgccgat ggcgcaaact cgatggtgcg gcgacatctc tacccggatc
    29821 atcaaatccg taaatatgtc gctatccagc agtggttcgc agagaaacat ccggtgccgt
    29881 tctactcctg catctttgat aatgcgataa ctgactgtta ctcatggagt atcagcaaag
    29941 atggttattt tatctttggc ggtgcctatc caatgaaaga cggtcagacg cgtttcacga
    30001 cgctgaaaga gaaaatgagc gcctttcagt tccagtttgg caaagcggtg aaaagtgaga
    30061 aatgcacggt gttgtttccc tcacgctggc aggattttgt ctgtggtaag gacaacgcct
    30121 ttctgattgg tgaagcggcg ggatttatca gcgccagctc gctggagggg attagctatg
    30181 cgctggatag cgcagagatt ctgcgtgcgg tgttcctgaa acagccggag aagagcaacg
    30241 ccgcttactg gcgcgccaca cacaaactgc gtttaaaact cttcggcaag atagtaaaaa
    30301 gccgatgcct gaccgcaccg gctttaagaa agtggattat gcgcagtggt gtggcgcata
    30361 ttccacagtt gaaagattat ccaacgcgct tcacatcacc caccagcagg atgtaagaga
    30421 gcgcgccaat caacgcgacg gcggagatat aaaccagtgc aggcgcgaaa ccgtaaccct
    30481 gcgccaggta ccccaccacc agcggaacgg tgatgccgcc cagaccaccg gcgaagttaa
    30541 acacgccgcc ggttaaacca atcaggcgca tcggtgccag agaagagacc agcgaccagg
    30601 taatcgaagc aaaaccgttg ccgaagaatg ccagcgccat caggcacata atcatcatcg
    30661 gatcgttggt gtagttagcg cccataatgc aggtggagat cagcaagccg cagataatcg
    30721 gcgttttacg cgcaaagccc agtgaaaagc ccttacgtac cagcagatcc gcgacccagc
    30781 cagagagcag gacgccgaca aacgccgcga ggaatggcac cgtggtcata aagcctgctt
    30841 tcagcgccgt gattcctttt tcctgggtta aatagttcgg gaaccaggtt aagaaaaacc
    30901 acagtgcaga agccaccgca aattgcccaa gataaacgcc gatcagttta cgatggaaca
    30961 ccagtttcca gtctttggct gttaacggct gacgcgcctc tttcttcacc ggcgcatcac
    31021 catccaccag accgccgcca tcacgaatgt aatccagttc agctttgctg atacctttgg
    31081 tcaggcgcgg cggctgataa accttaaacc aaatcagcga ccagataatg ccgataccac
    31141 cagtgacaat gaacacccag tgccagctca acatctcctg aatccagatc agcagcggag
    31201 tcaaaaacgc cagaccgaca aactgaccag acgtataaaa accaacggcg gaagcgcgtt
    31261 catgttccgg gaaccagctg gtcaccatcc ggttattggt cgggaaagcg ggcgcttcga
    31321 aaataccggt tatcgcacgc agaccaatta atgacattaa tcccgtggca aagccttgga
    31381 ataaagtcgc cactgaccaa ccaaatatcg caataaaata agtcacgcga gaacctacgc
    31441 gatctaaaaa ccaaccgccg gggatctggc atagcgtata aagccaggcg aaggccgaaa
    31501 atacatagcc catttccgct ttcgtaatgc cgaactcttc ctgaatatgg gcggaagcca
    31561 cggccaggtt agcgcggtca acataacaaa tgaccaccgt aataaagatc atcaccagcg
    31621 tcagataacg ccgacgcccc ggctttgctg cattaacggg aatatccata gcgagctttc
    31681 tccagatttt gggcatagcg aagccgctca ccatgccctg taatttacag agggttatat
    31741 ttttgtattg ctgttttagt gcccgatgag gggcttacgt ggcaggaatt accactctgc
    31801 tacgctgtta tcttcatgac gccagagcgg attacgccag tccggggcat ttttactgaa
    31861 ctcaataact ttagcttcgt cgatttccac acctaagccc ggtttcgtta acggtttaaa
    31921 gaagccgcca accatgctga agtcttcttt gtttttcaca aagtcgagta actccgcgcc
    31981 tttgttgtaa tgaatgccca tactttgtta ctgaagtacc gcgttatagg aaacgaagtc
    32041 gatatgcagg caagccgcca gtgcaatcgg tccgaacgga cagtgcggcg caagggtcac
    32101 atcataggct tctgccattc cagcaatttt gtagcattcg gtaataccgc ctgcatggga
    32161 gagatccggt tgcagaatcg aaataccgcc tgcctccagc acgcgtttaa aatcgaaacg
    32221 tgagaacatg cgctcacccg ccgccagtgg aatatgcgtt tgtgccgcca atttcgggta
    32281 gtattcggct tgttccgcca gcaccggctc ttcaataaac agcgggcgat acggctccag
    32341 ttctttaatc agcactttcg ccattggcgc gctgacgcga ccgtggaaat caagaccaaa
    32401 ctcaatctga ttgccaaaag cttcacgaat ttgtgccacg gtgttgactg ccgcatctac
    32461 cgcgcgggag ttatcaatta gccccagttc ttcacaaccg ttcagtttga aggtatcgaa
    32521 gccgatttcg cgcagggttt taatgccgtc gataacatcc gccggacgat cgccgccgac
    32581 ccaactgtag gctttaattt tgtcgcgaac caggccgccc atcagttgcc agactggcgc
    32641 attcagcact ttgcctttga tatcccataa cgcctggtcg atcccggcga tggcgctcat
    32701 caggattgga ccgccacgat aaaatccggc gcgatacatc acttgccata agtcgttgat
    32761 gcgcgaagga tcctgaccaa tcaaatagtc acccagctcg tgaactgccg cttccaccgt
    32821 acgggcgcgg ccttcaatca cgggctcgcc ccaaccgacc acgccttcat cggtttcaat
    32881 tttcaggaac atccagcggg gaggtaaacg atacgtggta attttggtga ttttcattgc
    32941 actgcctctc gatacgcctt aacaaatgct gctgcctgct gcgcggtgcg ttctacggac
    33001 tgcccggcgc gataaagatc gctgcctaag cccgccccaa cacaacctgc gtctatccac
    33061 tgcgccaggt tttctggcgt cacgccgcca acggcaaaga ctgcgatgtc cggtggtaat
    33121 accgctttta acgctttgat gtattgcgga ccaaaagccg atgacggaaa tattttcagc
    33181 gcctgcgcgc ccgcttcgag cgcggtaaag gcttcggtcg ccgtcgcgca gccggggcaa
    33241 acggtcatgc cgtagcccac cgcgcggcgg atcacttcac tatggatatt gggcgtaacg
    33301 atgagctgac agcccatcct ggcgagcgca tcgacctgtt caggtttcag taccgtacct
    33361 gcgccaatca acgccttgtc gccatacgca tcaacgatgg cgggaatgct ctgctcccat
    33421 tgtggggaat tcagcgggat ttcaaccgcg tcgaacccgg cgtcaatcac cgcgccaaca
    33481 tgcgccagcg cctcgtcggg cgtaatgccg cgcaaaatgg cgatcagcgg gagtttagtt
    33541 tgccactgca tgagcgatgc tccttatacc agcctgaaat gccgtgtcgc ccgccaccgc
    33601 cgccacgtcg caacccatcg cctgaaaggc ttgctggtag cgcgcggtca gcgatgttcc
    33661 ggcgacaagg gtgatggcgt gttgatgggt cacatagtcg cgcatactgg caacctctgc
    33721 gccaatcaac aaaccagaga gaaattcgct gacctgttcg cggggaagtg ttcccagcac
    33781 atgcgaggcg cgaacttcaa aaagctgcgg caatatggcg ggcgcattaa ggccgcgctc
    33841 aaggccagcc gcgaaggcat cggcagagtt ttcctgcggc ggcaaacctg cgccaatcaa
    33901 tgagtgattt aacagtaaat gatgtaattc accggtcatc acggtgcgaa aatcgttgat
    33961 ttgctggcta tcggcctgca cccatttgca atgcgtaccg ggcataacat aaagagagga
    34021 aggagccaga gctcgcgcgc cgatcaattg tgtttcttcg ccgcgcatca cattgtggtt
    34081 atcgtcatga gagacgcata atccgggaat aatccagata ttgtcgccaa cagacgttaa
    34141 ttgttcgcca atagacgaaa aacgggcagg aacagataaa tacggagcaa ctttccagcc
    34201 gacattgctg ccgaccattc ctgccatcac cactggcgta ttttcttcac gccagtcggt
    34261 cgtgacttct gctaacactg cagccggaga ttttccgttc aggcgcgtga cgcctgcttc
    34321 tgattgcctg ctctccaggc agtggtcgcc ctgataaagc caggcgcgca gattggtcga
    34381 tccccagtca attgcgatgt agcgagctgt catgtgattt cctttaacct tcgtgtcgag
    34441 ctggcaatca tggtaagcgc cgcctgctct gccgcatcgc cgtcctgatg ccgtatcgca
    34501 tcgaacagcg ccttatgttc ctggagcgtt tgcggcatgt tggcctcatc gcccatccag
    34561 gttcgttcaa aaaccgcccg ctgcagcgaa ctgatcgcaa tgctaagttg ctgtaacacc
    34621 gggttatgca ccgactgcag caccgcctcg tggtagcgaa tatccgcttc gttaaacgct
    34681 tcgcggtcct gattgttggc aatcatctcg ttcagcgccg attcaatctg cgccagatcg
    34741 ctggaggtcg cgcgctctgc tgcccaacgg gcaatcgccg gttccaccag atttcgcact
    34801 tcgctcatgg cactgataag ccgtgggtcg tagtcgtttt ccagcaccca ttgcagtacg
    34861 tcagtgtcga ggtaattcca ctggttacgc ggtgccacaa acgccccgcg ataacgtttc
    34921 atttcaatca gccgcttcgc catcagcgaa cggaacacct cacggatgat gttgcgcgag
    34981 gttgcaaact cttcacagag ctccgcctca gccgcaagtg gcgagccagg tacgtatttg
    35041 ccgtgaacga tctgtttacc cagcgtaatg acaatgcgat cggttttatt gagagtcatg
    35101 gagagtcctt gtgctctgta tgttcttctc tactttaccc cgatcgatgc ataacgcggc
    35161 aactttgtag taccagcgtg atgacgttcg cgtttgccgc gcgcgtaatg tagtacaaac
    35221 ttatattgtt gcgctacaat ttagatcaaa aaaagaacaa cacataaaaa aatgacatgc
    35281 gtcgggcaga aatctgaaaa gggatatcag gcgctaaaca ggagggaaag aagagtatgc
    35341 tttcaacggc ttagctactc gtttaaagga ttaatcatga agttgaattt taagggattt
    35401 tttaaggctg ccggtttatt cccactggcg ctgatgcttt caggctgtat ctcgtatgct
    35461 ctggtttccc ataccgcaaa gggtagttca ggaaagtatc aatcgcagtc agacaccatc
    35521 actgggctat cgcaggcaaa agatagtaat ggaacaaaag gctatgtttt tgtaggggaa
    35581 tcgctggatt accttatcac tgatggtgcc gatgacatcg ttaagatgct caatgatcca
    35641 gcacttaacc ggcacaatat tcaggttgcc gatgacgcaa gatttgtttt aaatgcgggg
    35701 aaaaagaaat ttaccggcac aatatcgctt tactactact ggaataacga agaagaaaag
    35761 gcactggcaa cgcattatgg ttttgcctgt ggtgttcaac actgtaccag gtcactggaa
    35821 aacctaaaag gcacaatcca tgagaaaaat aaaaacatgg attactcaaa ggtgatggcg
    35881 ttctaccatc catttaaagt gcgattttat gaatactatt cacccagagg cattccggat
    35941 ggtgtttccg cagcattact gccagtgact gttacactgg acatcattac tgcaccgctg
    36001 caatttctgg ttgtatatgc agtaaaccaa taatcagtaa gcgggcaaac gcgtttatgc
    36061 tgtttgcccg cccacagatt aattcagcac atacttctca atagcaaacg ccacgccatc
    36121 ttcaaggttg gattttgtga caaagttcgc cacttctttc accgaaggaa tagcgttatc
    36181 catcgccaca ccgacgcctg catattcaat cattgcgatg tcgttttcct gatcgccaat
    36241 cgccatgatt tcttccggtt taatacctaa cacgtcggcc agtgatttca cacccgtacc
    36301 tttgttaacg cgtttatcga ggatttcgag gaagtacggc gcacttttca gcacggtata
    36361 tttctctttc acttcctgcg gaatacgcgc gatagcctgg tcgaggatgg cgggttcatc
    36421 aatcatcatc actttcagga actgggtatt ggggtccatt ttctccgctt cgcagaacac
    36481 cagcggaatt gtggcaacga aggattcatg caccgtgtag tagctgatat cgcggttagc
    36541 ggtgtacagc gtggtgcggt ccagggcgtg gaaatgagaa ccgacttcgc gagagagttt
    36601 ttccaggaaa cgatagtcgt catagctgag agcggtttgc gccacggtgc taccatcagc
    36661 ggccttctgt accagcgcgc cgttataagt aatgcagtag tcgcccggct gttccatatg
    36721 cagctctttc aggtagttgt gcacacctgc atacggacga cccgtcgtta gcacgacatt
    36781 cacgccacgg gcgcgagctg cggcaatcgc atttttaacg gcgggtgaaa tggtgtgatc
    36841 gggcagcaga agggtgccat ccatatcgat agcaatgagt ttgatagcca tgagttccca
    36901 aggtagatta gttcctgacc catgctaacg cgattccgct caaaaatcag tacaacaccc
    36961 gagggaaaag gggatgcaac gcgggttgca tgctccccat ttgcttagcg gaagagtttc
    37021 cctttcagca gttccatgcc tgcggaaagc agatcgttat tggcttgtgc tgacacttca
    37081 ccttgcggtg agagcgcatc aataatcttc ggcaattgct ctgccagtaa actggaagcc
    37141 gtactggtat ccacgccaag tttttgcccg agatcggaca ccgcatttgt gccgagcgcc
    37201 gactccagtt gctcgccgct aactgattga tttctctgtt gattactcaa ccaggttgag
    37261 agaatggccc ctaagccgcc actttgcagt ttttccagca gcacctgaat gccaccctgc
    37321 tcctcaaccc aacttaaaat cgcctgatat ttccccgcat cgcctttcag aaaggcaccg
    37381 acaacttcat caaaaagccc catgataatc acctgtaaag cgttacgtgt tgacccaaaa
    37441 agtatagatc tgcgggatga taattgcgga ttgcagaaat aaaaagggcg gagatgatct
    37501 ccgccctttt cttatagctt cttgccggat gcggcgtgaa cgccttaccc ggcctacaaa
    37561 atcatgcaaa atcaatatat tgcaggagtc tcgtaggact aataagcgta gcgcatcagg
    37621 cacgctcgca tggttaacgc cattaaatat caatattcgc cgctttcagg gcgttctctt
    37681 caataaacgc acgacgcggt tctacggcgt cgcccatcag cgtggtgaac aactggtcag
    37741 cggcaatcgc atccttaacg gtaacgcgca gcatacgacg gctttccggg tccatggtgg
    37801 tttcccacag ctgttccggg ttcatctcgc ccagaccttt ataacgctgg atggagaggc
    37861 cacgacggga ctctttcacc agccagtcca gcgcctgctc gaagctggct accggctgac
    37921 gacgctcgcc acgttcgata aacgcatctt cttccagcaa gccacgcagt ttctcaccca
    37981 gcgtgcagat acgacgatat tcgccaccgg tgataaactc gtgatccagc ggatagtcag
    38041 tatccacacc gtgggtacgc acgcgaacaa tcggctcgaa caggttttgt tcggcattgg
    38101 tgtggacatc aaacttccac tggctgccgt gctgttcttt gtcgttcagt tcgctgacca
    38161 gcgcgttcac ccagcgggta acggtctgct catcagaaag gtcagcttcc gtcaacgtcg
    38221 gctgatagat aagctctttc agcattgctt tcggataacg acgctccatg cggttgatca
    38281 ttttctgcgt cgcgttgtac tcagatacca gtttctctaa cgcttcgcca gccaatgccg
    38341 gtgcactggc gttggtgtgc agcgttgcgc cgtccagcgc gatagagatc tggtactgat
    38401 ccatcgcttc gtcgtcttta atgtactgtt cctgcttgcc tttcttcact ttgtacagcg
    38461 gcggctgagc gatgtagacg tgaccgcgtt caacgatttc cggcatctga cgatagaaga
    38521 aggtcaacag cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga
    38581 tgctgtgata acgcagtttg tccgggttgt actcgtcacg accgatacca cagccaagcg
    38641 cggtgataag cgtcgccact tcctgagaag agagcatctt atcgaagcgc gctttctcga
    38701 cgttaaggat tttacccttc agcggcagaa tcgcctggtt cttgcggtta cgcccctgct
    38761 tcgcagagcc gcccgcggag tccccttcca ccaggtacag ttcggaaagc gccggatcgc
    38821 gttcctggca gtctgccagt ttgcccggca ggccagctaa gtcgagcgca cctttacggc
    38881 gggtcatttc acgcgcgcga cgcgccgctt cacgggcacg ggcagcatcg ataattttgc
    38941 caaccacgat tttcgcatca gttgggtttt ccagcaggta ttccgccagc agttcgttca
    39001 tctgctgttc aaccgccgat ttcacctcag aagaaaccag tttgtctttg gtctgggagg
    39061 agaatttcgg gtccggcact tttacggaaa cgaccgcaat caggccttca cgcgcatcgt
    39121 caccggtggc gctgactttg gcttttttgc tgtagccttc tttgtccatg taggcattca
    39181 gggtacgggt catcgccgca cggaagcctg ccaggtgagt accgccgtca cgctgcggaa
    39241 tgttgttggt aaagcagtag atgttttcct ggaagccatc gttccactgc agcgccactt
    39301 caacgccaat accgtctttt tcggtggaga agtagaagat atttgggtgg atcggcgttt
    39361 tgttcttgtt cagatattca acgaacgctt tgatgccgcc ttcatagtgg aagtggtctt
    39421 ctttgccgtc acgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg aacgacaact
    39481 cacgcagacg tttcgccaga atttcatatt cgaactcggt cacattggtg aaggtttcga
    39541 ggctgggcca gaaacgcacc atggtgccgg ttttttcagt ctcgccggta accgccagcg
    39601 gagcctgcgg tacaccgtgt tcgtagatct gacggtgaat tttaccctcg cgctgaataa
    39661 ccagctccag tttttgcgac agggcgttta ctaccgaaac accaacgccg tgcagaccgc
    39721 cggacacttt ataggagtta tcgtcaaatt taccgcctgc gtgcagaacg gtcatgatca
    39781 cttccgccgc cgatacgccc tcttccgggt gaataccggt cggaatgccg cgcccgtcat
    39841 cctgtacaga gacagagtta tcggcgtgaa tggtgacgat aatttcttta cagtgacccg
    39901 cgagcgcttc gtcgatagcg ttatctacca cctcgaatac catgtggtgc agaccggtgc
    39961 cgtcatccgt gtcgccgata tacatacccg ggcgcttacg caccgcatcc agccctttca
    40021 ggactttgat actggaggag tcataagaat tcgacatcaa cgtttctcgc tcatttatac
    40081 ttgggttaat ccgttatttt acccttttcc acggtaaaca tcttcgaatt ttcgtccgac
    40141 atgtctataa cgtgttcagc actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt
    40201 aagcggctgg caagcaaccc gcgacgctca tcatcaagct cagaggcaaa atcatctatc
    40261 aggtagagac accgccgccc gctttcacga gtgaggaact ctccttgcgc cagacgtaag
    40321 gcgcacatca acagcttaag ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg
    40381 gcgcaaatgc gtaagtccgc tttatgcggg ccatgcgcgg tgtaggttag ctggcgatcg
    40441 cgttcaaaat tacgttccag cacctcagcg tactctgtct ctttctccca gccgcgctgg
    40501 aaagagaaag tcagagaaaa ctcagggaga aattgcttac aggtatcatc catatcggcc
    40561 gcgataccgg cgctatactc cgcgcgccag gtgctgattt gctccgccag cgggatcagt
    40621 tctttatccc acgggcgtag ctgttcgtaa cgagtcacct ggcgcagcgc cgcattgcgc
    40681 tgcttgagca atcgcttgag attgctccag gcggtgaaaa atccaggttc gttgtgaaag
    40741 catccccagt cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa agtaaaccct
    40801 tctggcgtta tcaactgcat cggcatcagg tgcgccagtt ccgcgacttt atgcccgtcc
    40861 gtaccgtcaa tacggacttt gctgtcgccc tgtttgtctt tggttaagcc aatcgccgtc
    40921 tcgcgctctt cgccctgtaa tcgcccgtgg agaacaaatg cctcctgctc atggcgaatg
    40981 acgcgaccaa tctgcaaact gcgaaacgcc cgaccatggc cgagcgtata gatggcttcc
    41041 agcacgctgg ttttgccact gccgttggca cctaccagaa agttaaagcc gggagataaa
    41101 gcgagatccg cggtttcaat attgcggaaa tcgcggatca acaagcgggt gagggacatt
    41161 acagtctcat tggcatgaca acataggcag ccgactgtga tgcggcatct tcaatctgca
    41221 cgctggaaac cgaatcggtc agcatcatgc ggacgttttc gcatttcagc gcgttcagaa
    41281 catccagcac atagctgacg ttgaagccga tttccatctc cgcaccgcta taggtaacgt
    41341 cgaggatctc ttccgcttct tcctgttccg ggttgttggc agtaattttc agctggtttt
    41401 cgctgacata caggcgcaca ccgcggaatt tctcgttaga gagaatcgcc gcgcgagcaa
    41461 acgcctgctt gagcagatcg cagcctgctt ccagatgttt gtccgggttc ttcggcagaa
    41521 cgcgacggta atccgggaag cgaccatcca ccagtttcga ggtgaaaata aagtcgccaa
    41581 cgtgggcacg aatgttattg ctgccaatct gcacacgcag cagattgtcg ccgccgtcga
    41641 gcatacgcat cagttcaatc acgcctttac gcggtacgat caccgaatgg cttggcaaag
    41701 attgaccaat tggcattgaa cagaccgcca gacggtggcc gtcggttgcc acggtgcgca
    41761 gttcttcacc ttcggtttca aacagcatac cgtttaagta atagcgaaca tcctgatgcg
    41821 ccatcgaaaa ctgggttgct tcaatcagac gcttcatcgt cgcctgcggc agggtaaatt
    41881 cgacttcgct ctgccagtca tcgaggttcg ggaaatccgc cgctggcagg gtagacagcg
    41941 aaaaacggct acgcccggag cgcaccagca tccgttcacc ttccagctgc acggcaattt
    42001 ccgcgccttc aggcagacca cggcagatat caaagaattt gcgcgccgga acggtcgtcg
    42061 ctcctggctc gtgtggctga accagcgcaa cgcgtgccac catttccatc tcgagatcag
    42121 taccggtcag cgacaacgta ccgtcagcaa cctgtaacag cagattaccg agaatcggta
    42181 gcgtaggacg accacctaac ggaccgctca cctgttgtag cggttttaat aaatgctcac
    42241 gttctacggt aaatttcata ggtttacgat gacaatgttc tgattaaatt tgaaaaatct
    42301 tctttgatat cgtggctctc ttcacgcaac tgctcgatct tacggcaggc atgaagcacc
    42361 gtcgtgtggt cacgcccacc aaacgcatcg ccaatctctg gcagactgtg gttagtcagc
    42421 tctttcgcta gcgccatcgc catctggcgc ggacgcgcca ccgagcggga tcgacgcttg
    42481 gaaaggagat ccgcgacttt gatcttgtag tactccgcca ccgtcttctg aatattgtcg
    42541 atggtgacca gtttttcctg caatgccagc aagtcgcgca gcgcctcacg cacgaagtcg
    42601 atggtgatcg cccgtccggt aaagttggcg ttagcaataa cgcggttcag cgccccttcc
    42661 agctcacgta cgttagatcg tagacgcttg gcgataaaga acgccacttc acccggcaaa
    42721 cgaatgtcat tttcgtcggc ctttttcatc aggatcgcca cacgggtttc cagctctggc
    42781 ggttcgatcg ccacagtcag tccccaaccg aagcgggatt tcaaacgatc ctcaacgccg
    42841 ttgatctctt tcggatagcg atccgaggtg agaatgatct gttgattacc ttccagcagg
    42901 gcgttgaagg tgtggaaaaa ctcttcctga gatcgttctt tattagcaaa aaactgaata
    42961 tcgtcgatca gcagtgcatc tacggaacgg tagtagcgtt taaactcttc gatcgcgttg
    43021 ttttgcaggg ctttaaccat gtcctgaaca aagcgctcgg agtgcatata aaccactttg
    43081 gcattcggct tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg agttttaccc
    43141 agacccgtgc cgccataaag gaacaacggg ttataggcac cgccagggtt atccgccacc
    43201 tggcaagccg ccgcgcgcgc cagttggtta gatttacctt caacgaagtt atcaaacgtg
    43261 tgtttgacgt ttacgttaga acgataggtc ggttctgccg gggccgggac gttatcccaa
    43321 cctgagcgcg tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc
    43381 gcgacgttgc tcgtcactgc cgcttgtggc gtttgcgtca ccggtttggt gccgacttca
    43441 aaacgcagct gtggggcatc cgctccgcag aaactggtta gcagtccatt gatattatta
    43501 aggtacttgt cccgtaccca atcgaggaca aaacggtttg gcgcgtacag ggccagcgtg
    43561 ttatcgctca gttccgcctg caatgggcgt atccacatac tgaattctgt ggctggtaac
    43621 tcatcctgca atcgggcaag acactgctgc caaagcgaaa gtgacacggc ggactccact
    43681 cgaacaaaag tcgataatga ctaaggctga aacattcatg attgttgacg tacgtcgaaa
    43741 agaccctgct tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc
    43801 ctgcaaaacg atcgggaccg cggatcatag cctaaactgc gcaagagatc ttctgtttct
    43861 cacagatttt ccctatttat ccacaggact ttccagaact cgctaagtgt aaacgatcct
    43921 gccgcgaggc gggcacgatt tacgccgcat attggaaaat ttaatgacca tagacaaaaa
    43981 ttggcttaat cgatctaata aagatccagg acgatccttg cgctttaccc atcagcccgt
    44041 ataatcctcc acccggcgcg ccatgctggt ttccactggt gtgaggtcgt acattttccc
    44101 tgcgaaaagg tgcggaaaag cgcggtaaat aaggaaagag aattgactcc ggtgtgtaca
    44161 attattacaa tccggcctct ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc
    44221 ggcggatatc caataaagcc attgaattta ttcaagttta ggtagaaatc gccatgaaac
    44281 gcacttttca accgtctgta ctgaagcgca accgttctca cggcttccgt gctcgtatgg
    44341 ctactaaaaa tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga
    44401 ccgtttctaa gtaataaagc taacccctga gtggttaagc tcgcatttcc cagggagtta
    44461 cgcttgttaa ctcccagtca attcacattc gtcttccagc agccgcaacg ggctggcacg
    44521 ccgcaaatta ccattctcgg ccgcctgaat tcgctggggc atccccgtat cggtcttaca
    44581 gtcgccaaga aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg cctgacgcgt
    44641 gaaagcttcc gtctgcgcca acatgaactc ccggctatgg atttcgtggt ggtggcgaaa
    44701 aaaggggttg ccgacctcga taaccgtgct ctctcggaag cgttggaaaa attatggcgc
    44761 cgccactgtc gcctggctcg cgggtcctga tagccctcat tcgggtctat caacgcctga
    44821 ttagtccgct actcgggccg cattgtcgtt tcactccaac ctgttcaagc tacggaattg
    44881 aggcattgcg caggtttgga gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa
    44941 aatgccaccc tttacaccct ggtggtgacg atcccgtccc gcccggacca tttgatacca
    45001 gagaacacta acgatggatt cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc
    45061 tttcatgatc tggcaagcct gggagcagga taaaaacccg caacctcagg cccaacagac
    45121 cacgcagaca acgaccaccg cagcgggtag cgccgccgac cagggcgtac cggccagtgg
    45181 ccaggggaaa ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca acacccgtgg
    45241 tggtgatgtt gagcaagctc tgctgcctgc ttacccgaaa gagctgaact ctacccagcc
    45301 gttccagctg ttggaaactt caccgcagtt tatttatcag gcacagagcg gtctgaccgg
    45361 tcgtgatggc ccggataacc cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga
    45421 cgcttatgtg ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc
    45481 ggcaggcaac acgtttacca aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt
    45541 caactacaac gtgcagaacg ctggcgagaa accgctggaa atctcctcgt ttggtcagtt
    45601 gaagcaatcc atcactctgc caccgcatct cgataccgga agcagcaact tcgcactgca
    45661 caccttccgt ggcgcggcgt actccacgcc tgacgagaag tatgagaaat acaagttcga
    45721 taccattgcc gataacgaaa acctgaacat ctcttcgaaa ggtggttggg tggcgatgct
    45781 gcaacagtat ttcgcgacgg cgtggatccc gcataacgac ggtaccaaca acttctatac
    45841 cgctaatctg ggtaacggca tcgtcgctat cggctataaa tctcagccgg tactggttca
    45901 gcctggtcag actggcgcga tgaacagcac cctgtgggtt ggcccggaaa tccaggacaa
    45961 aatggcagct gttgctccgc acctggatct gaccgttgat tacggttggt tgtggttcat
    46021 ctctcagccg ctgttcaaac tgctgaaatg gatccatagc tttgtgggta actggggctt
    46081 ctccattatc atcatcacct ttatcgttcg tggcatcatg tacccgctga ccaaagcgca
    46141 gtacacctcc atggcgaaga tgcgtatgct gcagccgaag attcaggcaa tgcgtgagcg
    46201 tctgggcgat gacaaacagc gtatcagtca ggaaatgatg gcgctgtaca aagctgagaa
    46261 ggttaacccg ctgggcggct gcttcccgct gctgatccag atgccaatct tcctggcgtt
    46321 gtactacatg ctgatgggtt ccgttgaact gcgtcaggca ccgtttgcac tgtggatcca
    46381 cgacctgtcg gcacaggacc cgtactacat cctgccgatc ctgatgggcg taacgatgtt
    46441 cttcattcag aagatgtcgc cgaccactgt gaccgacccg atgcagcaga agatcatgac
    46501 ctttatgccg gtcatcttca ccgtgttctt cctgtggttc ccgtcaggtc tggtactgta
    46561 ctatatcgtc agcaacctgg taaccattat tcagcagcag ctgatttacc gtggtctgga
    46621 aaaacgtggc ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa
    46681 ataagggcgg tcagttgacc gccttttttc ttttcgtagg gcggataagc accgcgcatc
    46741 cgccacacaa agcaacagga acatcatgag cgataatgac actatcgtag cccaggccac
    46801 gcctccggga cgtggcggcg ttggcatcct gcgcatctcc ggcttcaaag cccgtgaagt
    46861 tgccgaaacc gtgctgggta aactgcctaa gccgcgctac gccgattatc ttccgtttaa
    46921 agacgccgac ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg gcccgaactc
    46981 gttcaccggc gaagatgtgc tggaactgca aggtcatggc ggtccggtga tcctcgacct
    47041 gctgttaaaa cgcattctga ccattcccgg cctgcggatt gctcgccctg gtgagttttc
    47101 cgaacgcgcg tttcttaacg ataaacttga cttagcccag gccgaggcga ttgccgatct
    47161 tatcgacgcc agttcggaac aggcggcccg ttcggcactt aactcgctgc aaggcgcatt
    47221 ctccgcacgg gttaatcatc tggtagaagc cctcacccac ttgcgcattt acgtcgaagc
    47281 ggcaattgat ttccctgatg aagagatcga tttcctctcc gacggcaaaa ttgaagccca
    47341 gcttaatgac gtgatggccg atctcgatgc agtgcgtgct gaagcacgtc agggtagttt
    47401 gttgcgcgaa gggatgaaag tagtgattgc cggacgtcct aatgccggta aatcgagcct
    47461 gttaaacgcg ctggcgggcc gtgaagcggc aatcgtaacc gatatcgccg gaaccacgcg
    47521 tgacgtgctg cgtgagcata tccacattga cggaatgccg ctgcatatca tcgacactgc
    47581 cgggctacgt gaggccagtg acgaagtgga acgtattggt atcgagcgcg cgtggcagga
    47641 aattgaacag gccgaccgcg tgctgtttat ggtcgatggc accacgacag acgccgttga
    47701 tccggcagag atctggccgg aatttatcgc ccgtctgccc gcgaaactgc cgatcaccgt
    47761 ggtacgcaat aaagccgata tcaccggcga aacgctggga atgagtgaag tgaacggtca
    47821 cgcgttaatt cgtctctcgg caaggactgg tgaaggcgtg gacgtgctgc gtaaccatct
    47881 caaacagagc atgggctttg acaccaatat ggaaggcggc ttcctggcgc gccgtcgcca
    47941 cttacaggcg ctggaacagg cagcggagca tttgcagcaa ggtaaagcgc aactgttggg
    48001 agcctgggca ggtgaactgc tggcggaaga gttgcgcctg gcacagcaga acttaagcga
    48061 aatcaccggg gaatttactt cagacgacct gctggggcgg attttctcca gcttctgtat
    48121 tggtaagtaa ccgcgcttac gaagccgtat tctgactgtc agatgcggct tcgcttcatt
    48181 gttagcactc ctgttatgcc tcaacccttt tttaaacatt aaaattctta cgtaatttat
    48241 aatctttaaa aaaagcattt aatactgctc cccgaacgat tgtgattcga ttcacattta
    48301 aacaatttca gaatagacaa a
//
LOCUS       DAJTSF010000019        47956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000019 DAJTSF010000000
VERSION     DAJTSF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.019"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO335_001177"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO335_001177"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6001642.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="OO335_001178"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="OO335_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6001643.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO335_001179"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO335_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6001644.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO335_001180"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO335_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6001645.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO335_001181"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO335_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6001646.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="OO335_001182"
     CDS             5377..5835
                     /locus_tag="OO335_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6001647.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="OO335_001183"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="OO335_001183"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6001648.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="OO335_001184"
     tRNA            6887..6971
                     /locus_tag="OO335_001184"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="OO335_001185"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="OO335_001185"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO335_001186"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO335_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6001649.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO335_001187"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO335_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6001650.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO335_001188"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO335_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6001651.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="OO335_001189"
     CDS             complement(10252..13995)
                     /locus_tag="OO335_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6001652.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO335_001190"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO335_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6001653.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="OO335_001191"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="OO335_001191"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6001654.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="OO335_001192"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="OO335_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6001655.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO335_001193"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO335_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR6001656.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="OO335_001194"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="OO335_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6001657.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO335_001195"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO335_001195"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6001658.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO335_001196"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO335_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6001659.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="OO335_001197"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="OO335_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6001660.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="OO335_001198"
     CDS             23392..23499
                     /locus_tag="OO335_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6001661.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO335_001199"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO335_001199"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6001662.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="OO335_001200"
     CDS             complement(24538..25347)
                     /locus_tag="OO335_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR6001663.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="OO335_001201"
     CDS             complement(25351..25743)
                     /locus_tag="OO335_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6001664.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO335_001202"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO335_001202"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6001665.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO335_001203"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO335_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6001666.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO335_001204"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO335_001204"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6001667.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="OO335_001205"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="OO335_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6001668.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="OO335_001206"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="OO335_001206"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6001669.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="OO335_001207"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="OO335_001207"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6001670.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="OO335_001208"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="OO335_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6001671.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO335_001209"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO335_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6001672.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="OO335_001210"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="OO335_001210"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6001673.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="OO335_001211"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="OO335_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6001674.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="OO335_001212"
     CDS             36790..39657
                     /locus_tag="OO335_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6001675.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO335_001213"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO335_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR6001676.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="OO335_001214"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="OO335_001214"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR6001677.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="OO335_001215"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="OO335_001215"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR6001678.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="OO335_001216"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="OO335_001216"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR6001679.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="OO335_001217"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="OO335_001217"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6001680.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /locus_tag="OO335_001218"
                     /pseudo
     CDS             complement(47142..47581)
                     /locus_tag="OO335_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12042 a        12343 c        11743 g        11828 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcattcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat gcgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgtttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggaag
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgccagttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtagg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgtcaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccta acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacaatta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaaatcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tagtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc atactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taattaaaaa aggattctgt tgttgttacc gggaaccgac
    47641 ttatttcgct cagaaagtcg ttcaaccagc tccgcagtga taagattcgc tgatgataca
    47701 gtgtatcctc actgcgtgct ggaggtgcca gtataatatg ttgatcaata cagtatttat
    47761 tgtgttcagt cgtttcctga tgcgtttggt tctctgcgga ttgtcgctgg cgaaataaat
    47821 ggcttgcggt aaatgccgtt acagagcagc gcaacgcgtc aattccttcg gagtgcccga
    47881 cttttggcga gagttccggt tttatgcgtc caggtagcgg aaatttcacc cgaatgagaa
    47941 atgcattaat aaaatg
//
LOCUS       DAJTSF010000020        47512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000020 DAJTSF010000000
VERSION     DAJTSF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.020"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            172..654
                     /gene="sseA"
                     /locus_tag="OO335_001219"
                     /pseudo
     CDS             172..654
                     /gene="sseA"
                     /locus_tag="OO335_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="OO335_001220"
     CDS             complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="OO335_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6001681.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="OO335_001221"
     CDS             complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="OO335_001221"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6001682.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="OO335_001222"
     CDS             complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="OO335_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6001683.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="OO335_001223"
     CDS             complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="OO335_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6001684.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="OO335_001224"
     CDS             complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="OO335_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6001685.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="OO335_001225"
     CDS             complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="OO335_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6001686.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="OO335_001226"
     CDS             complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="OO335_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6001687.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="OO335_001227"
     CDS             complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="OO335_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6001688.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="OO335_001228"
     CDS             complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="OO335_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6001689.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKQPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="OO335_001229"
     CDS             complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="OO335_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6001690.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="OO335_001230"
     CDS             complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="OO335_001230"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6001691.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10457..11260
                     /gene="suhB"
                     /locus_tag="OO335_001231"
     CDS             10457..11260
                     /gene="suhB"
                     /locus_tag="OO335_001231"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6001692.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11405..12259
                     /locus_tag="OO335_001232"
     CDS             11405..12259
                     /locus_tag="OO335_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6001693.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12450..13730
                     /gene="csiE"
                     /locus_tag="OO335_001233"
     CDS             12450..13730
                     /gene="csiE"
                     /locus_tag="OO335_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6001694.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13722..14861)
                     /locus_tag="OO335_001234"
     CDS             complement(13722..14861)
                     /locus_tag="OO335_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6001695.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="OO335_001235"
     CDS             complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="OO335_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR6001696.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            16047..17408
                     /locus_tag="OO335_001236"
     CDS             16047..17408
                     /locus_tag="OO335_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6001697.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17405..17923
                     /locus_tag="OO335_001237"
     CDS             17405..17923
                     /locus_tag="OO335_001237"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6001698.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17923..18243
                     /locus_tag="OO335_001238"
     CDS             17923..18243
                     /locus_tag="OO335_001238"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6001699.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18240..19052
                     /gene="hcaB"
                     /locus_tag="OO335_001239"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18240..19052
                     /gene="hcaB"
                     /locus_tag="OO335_001239"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6001700.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            19062..20279
                     /locus_tag="OO335_001240"
     CDS             19062..20279
                     /locus_tag="OO335_001240"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR6001701.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            20376..20798
                     /locus_tag="OO335_001241"
     CDS             20376..20798
                     /locus_tag="OO335_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6001702.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20846..21718)
                     /locus_tag="OO335_001242"
     CDS             complement(20846..21718)
                     /locus_tag="OO335_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6001703.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21730..22824)
                     /locus_tag="OO335_001243"
     CDS             complement(21730..22824)
                     /locus_tag="OO335_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6001704.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22857..23855)
                     /locus_tag="OO335_001244"
     CDS             complement(22857..23855)
                     /locus_tag="OO335_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6001705.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23880..25391)
                     /locus_tag="OO335_001245"
     CDS             complement(23880..25391)
                     /locus_tag="OO335_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6001706.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25414..26396)
                     /locus_tag="OO335_001246"
                     /pseudo
     CDS             complement(25414..26396)
                     /locus_tag="OO335_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26493..29594)
                     /locus_tag="OO335_001247"
     CDS             complement(26493..29594)
                     /locus_tag="OO335_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR6001707.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29601..29774)
                     /locus_tag="OO335_001248"
     CDS             complement(29601..29774)
                     /locus_tag="OO335_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001708.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29892..31085
                     /locus_tag="OO335_001249"
     CDS             29892..31085
                     /locus_tag="OO335_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR6001709.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31283..32536)
                     /gene="glyA"
                     /locus_tag="OO335_001250"
     CDS             complement(31283..32536)
                     /gene="glyA"
                     /locus_tag="OO335_001250"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6001710.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32863..34053
                     /gene="hmpA"
                     /locus_tag="OO335_001251"
     CDS             32863..34053
                     /gene="hmpA"
                     /locus_tag="OO335_001251"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6001711.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(34098..34436)
                     /gene="glnB"
                     /locus_tag="OO335_001252"
     CDS             complement(34098..34436)
                     /gene="glnB"
                     /locus_tag="OO335_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6001712.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34497..35831)
                     /gene="glrR"
                     /locus_tag="OO335_001253"
     CDS             complement(34497..35831)
                     /gene="glrR"
                     /locus_tag="OO335_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6001713.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35821..36534)
                     /gene="qseG"
                     /locus_tag="OO335_001254"
     CDS             complement(35821..36534)
                     /gene="qseG"
                     /locus_tag="OO335_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6001714.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36699..38126)
                     /gene="qseE"
                     /locus_tag="OO335_001255"
     CDS             complement(36699..38126)
                     /gene="qseE"
                     /locus_tag="OO335_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6001715.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38684..42571)
                     /gene="purL"
                     /locus_tag="OO335_001256"
                     /gene_synonym="purI"
     CDS             complement(38684..42571)
                     /gene="purL"
                     /locus_tag="OO335_001256"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6001716.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42830..44386
                     /gene="mltF"
                     /locus_tag="OO335_001257"
     CDS             42830..44386
                     /gene="mltF"
                     /locus_tag="OO335_001257"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6001717.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44383..44919)
                     /gene="tadA"
                     /locus_tag="OO335_001258"
     CDS             complement(44383..44919)
                     /gene="tadA"
                     /locus_tag="OO335_001258"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6001718.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44944..45579)
                     /gene="yfhb"
                     /locus_tag="OO335_001259"
     CDS             complement(44944..45579)
                     /gene="yfhb"
                     /locus_tag="OO335_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6001719.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45788..46636
                     /locus_tag="OO335_001260"
     CDS             45788..46636
                     /locus_tag="OO335_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6001720.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46975..47512)
                     /locus_tag="OO335_001261"
                     /pseudo
     CDS             complement(46975..47512)
                     /locus_tag="OO335_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11328 a        13021 c        11954 g        11209 t
ORIGIN      
        1 acgttacaga cggtcccggc aaaagtgcct gacctgaccc ctattagcgg ttaccagcgc
       61 cccaccagac ccggtgacat ccccccccgg atcatggacg actgggggca gtaatcatgc
      121 cgggtctggc tggctaacac cccattataa ggggtacgaa taaagtaacg gggacttgca
      181 ggctggcagc gcgatgacct gctattagaa gaaggtgcag tagagctgcc ggaaggagag
      241 tttaacgccg cgtttaatcc tgaagcagtg gtgaaagtaa ccgatgtgtt attggcaagc
      301 catgaaaata cggcgcaaat tattgatgcc cgcccggctg cacgttttaa cgcagaagtt
      361 gatgaacctc gcccaggttt acgtcgcgga catattcccg gtgcattgaa cgttccgtgg
      421 acggaactgg tgcgcgaagg cgaactaaaa acgaccgatg aactggatgc gatatttttt
      481 ggtcgcggcg tcagctatga caaaccgatt atcgtcagct gcggctctgg tgtaacggca
      541 gccgtggttt tgttagcact cgcgacgctg gatgtgccaa acgtgaaact gtacgacggc
      601 gcatggagtg aatggggcgc gcgggcagat ttaccggttg agccagtgaa ataagtatat
      661 tacaggcaat aaaaaaccgc cgaatttggc gtttttttat tgctggtctg gttcgcggcc
      721 tttccagcag gttgacttgt gttacatgag caacgcaggt gcttcacagc aaaacaatac
      781 tcaccagtaa ctctcttttt gtcaagcaaa agagagtaat tattgtttat ttagcgtatt
      841 atcgacaccg gccctttccg ccgtgttcgg taataaaata acctggctta ttagtccgaa
      901 ttcagacaaa tataaacaaa tcctgctcaa aattaaaaat tctaaccggt aaaagatatt
      961 acttaaacat gtaaatttac tttcctttaa aaaacaaaaa accgccaaaa tcaggcggtt
     1021 atttgttgct ggtccggttc gcggcctttc cagcaggttg tattaccgta gtaatgcaag
     1081 cgcgtctcag cggagacaat actcgccagt aactctcttt ttgtcaagca aaagagagtt
     1141 attattgttc tgttagtgta ttatccactg cggccctttc cgccgtctcg caagcgggcg
     1201 ctggcttaag gaaaggatgt tccgcgaccg ttagagcaag cgcgtctcag cgcttgctat
     1261 cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt cgcatggacc gcacaatgaa
     1321 gatacggtgc ttctgtatcg tacttattgt ttctggtgcg ctgttttccc aagtaaataa
     1381 taaccggagt ctctccggcg acaatttact ggtggttaac aaccttcaga gcagcaagta
     1441 agcccgaatg ccgcccttta gggcggcata ttttagatta tccggttttg tttaaagtca
     1501 cgcaaaaaac caccccagcg acgttcatag aatggcgcaa tatgttcggt aataaagtgg
     1561 ctaattcctt tttccccttt tttcacctga caaatatcga ttggttcatc gccaggtaat
     1621 gtatcggtcg ctacacttcc cgtcgcctga ataatttctt cgatatcacc atcggcttca
     1681 atgccaataa gtaaattagg ctgtgcctct tcgttctctt taattgaaca aataaaagca
     1741 cgcttcaccg gcttaatggt tttaaataag gtggtgagtg aatcaatcat ttgtgctggc
     1801 ggctctgcga cttccgataa tatcagcgat tcaccgcctt ccaggatttc ctggctgctc
     1861 agcggatttc cctcttcacc aatcaacaaa ctgatttcac gcggcataaa ttctttaccg
     1921 gtcggcagtt tggcattaag gaagagcgtt tcgccaaggg tcatctcaaa caatgtgcga
     1981 acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct gttgaagcgc ttctaacgag
     2041 gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc agtgctgtaa atcaagcgca
     2101 ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag gcacccagac ggtggattcc
     2161 agtagagtac ggaaaaaggc cgggcggtgc gccggttcgg ttgctgcttt ttccagcagg
     2221 tcttcaagtt cgttttttgt ttcggacata agaaccacaa ttcattcaac gttcggggcg
     2281 caaatgcctg atgcgctacg cttatcaggc ctacaaggga ttcgcaattt gttgaatttg
     2341 cagaatttgt aggccggata aggcgtttac gccgcatccg gcatattagt ttacgccgtt
     2401 aacagattag ctatcgtgcg cacaccaagt cccgtagcgc ccgcagacca ctgttcaacc
     2461 ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc agccttgctg atagttctca
     2521 acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg ccggatacgc cgcgcttccg
     2581 gtattgttca gttcggcaaa gttagacggc agctggctgc ggtggaattc cgccagcggc
     2641 agacgccaga atggttcgtt ctcttgtgag gcactcgcca gtaaacgacc ggcaagcgca
     2701 tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg cagttttcgc cgccccggtg
     2761 agggtcgccg catcaatgat catttccggt ttctgcgcac tggcatcaat cagaccatcg
     2821 gcaagcacca gacgcccttc ggcatcagtg ttcatcactt caactttttt accgttgcga
     2881 taggtgatga tatcgcccag cttgaacgca ttgccgctaa tcaggttatc cgcacagcag
     2941 aggaacagct tcacgcgctt gttcagtccg cgcataatgg caaatgccag cgccccggta
     3001 accgttgccg cgccgcccat gtccgacttc atcgagtcca taaacgcagt ctgtttgatg
     3061 ctgtagccgc cggagtcaaa agtgatacct ttacctacca ggcacgcgta caccggtgct
     3121 tctttatcgc cagttgggtt gtaatccagc gccagcaata ccggagaacg ttctgaaccg
     3181 cgcccgacag tgtgcagccc catataacct tgctcacgca gatcttcgcc tttggtgatc
     3241 cgataagtca cacgatcgcc cgcgacgttg ctgatcagat caacagcacg ctgtgccagt
     3301 tgcgatggtc ccaattcttc tgccggtgcg ttgatggtgt cacgcaccca gtcgatgatc
     3361 atcaggcggt tatccagttc ctggcgctgg gcatcgtcca gatccggcca ctccacttta
     3421 cgcgtgcctt tcgggccttt ataaccttgc cagaatgccc agcagcgatc cgcatcccag
     3481 ccttcaccgc ttaactgaac atgcttgatg cccagaccgt caatcttgcg ggccgcacgc
     3541 tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg taatgccgtc attattaatg
     3601 ctgtaagttg ctttttctcc ccagcgcgcg tcggcaggtt gggtagagag ggtaatcttc
     3661 atcgcttctg tcattttagt tatccttatt cgtttaacag caaaacgggc tacccccagg
     3721 cagcccgttc attcactcgg cttcgtccag ccagactaac aaaatcgctt cgaggatttt
     3781 ctcgttggat gcctgcgggt cgtcgtcgaa atcttccaga tcgcaaatcc actgatgcat
     3841 atcggtgaat cgaaccgttt tcggatcaag atcgggatac gcatcgtaca gtgcttcgcc
     3901 aatttcgcgg ctatcggtcc acttaagtcc catactaacc tctgttaatg ctcacgcgca
     3961 tggttgatag tgtaacgcgg gatttcgacc actaaatctt cgtcggtgac gcgcgcctgg
     4021 cagcttaaac ggctttccgg ctccagtccc caggctttgt ccagcatgtc gtcttcctgc
     4081 tctgagcttt ccggcagtga gtcaaaacct tcacgaacga tgcagtggca ggtggtgcaa
     4141 gcacaggatt tttcacaggc gtgttcaatc tcaataccgt tacgcagcgc tgcgtcgaga
     4201 atggtttcac cgctattagc ttccagaaca gcgccatcag ggcagaggtc ctgatgaggc
     4261 aaaataacaa tctttggcat attaaacctc gtccacggaa tggcctttca gcgcacgacg
     4321 aaccgactgg tccatgcggc gagcggcgaa atcctgggtt tgtttgtcta cgtttttaat
     4381 cgcttgttcg atggcgtcaa catcatcgcc ctgcgccact tcactcaggt gagcggcagc
     4441 attgtcaatg acctgacgct ctgcggcgct taacagcgcg gcatcagcag ccagcgcgcc
     4501 gtgcagactt tccagcacac gcgccgcttc tactttttgt tctgccagca tacgggcttt
     4561 tacgtcctgc tcggcatagc tcattgagtc tttgatcatc gaagcgattt cgctatcggt
     4621 cagaccgtaa gacggtttga cctgaataga cgcctcaacg ccggtggatt tctccatcgc
     4681 cgtcacgctc aaaagaccgt cggcatcgac ctggaacgtc acgcgaatat gcgcaccgcc
     4741 agccggtagc gccggaatac cacgcagcgc aaaacgcgcc agtgagcggc agtcctgcac
     4801 cagttcgcgc tcaccctgca ttacatggat agacatcgcc gtctgaccat ctttaaaggt
     4861 ggtgaaatcc tgagcgcggg ccaccggaat agtggtatta cgcggaatca ctttctccac
     4921 caggccgccc atcgtttcga ggcccagcga cagtgggatc acatcaagca gcagcatttc
     4981 gctgtctggc ttgttaccca ccagaatatc cgcctgaatc gccgcgccaa tagcgacgac
     5041 tttatccggg tcgatggaag tcagcggtgg acgaccgaaa aattcgccta cccgttcacg
     5101 caccagcggc acgcgagtag aaccgcccac catcaccact tccagcactt cgtcagcttc
     5161 tacacccgcg tctttcagcg cgcgacgaca agccagtaag gttcgtttta ccagtggcgc
     5221 gatcagttca ttgaattgtt cacggctgat ttcgccctgc cagcccgcaa cgttaacggt
     5281 cacggagtcc gcatcgctca gcgcgatttt ggctgcaatg gtggcatcca gcagttcacg
     5341 ctgaacgcgg ttatcgctac gatcaggaat gtccgcctgc tcgcgaatgt aatccgccag
     5401 cagatggtcg aaatcatcgc cgccgagcgc ggaatcaccg ccggttgcca gcacttcaaa
     5461 cacgccgcga cttaagcgca gaatggaaat atcaaacgtc ccgccaccga ggtcataaac
     5521 agcgatcacg ccttcctgac cggaatccag cccgtaggcg atagccgcag cagtcggttc
     5581 gttaagtaag cgcagaacat gcaaccccgc cagacgcgcc gcgtctttgg tgccctgacg
     5641 ctgggcatcg tcaaagtacg ccggaacggt gataactaca ccatccagct cgcctgccag
     5701 ggcttcagtt gcccgcgccg ccagtgcttt gaggatgtcc gcagaaacgc gcaccgggtt
     5761 cagtagcccc gccgccgttt caatcatcgg caggccgttt tcgctggcct ggaattgata
     5821 aggcagatgc ggatagcgtt gctggatatc agccagcgag cgccccatca ggcgtttaac
     5881 agaactaatg gtgttggcgg tgtcaagcgc tgcgttagta cgcgcgtcat aacccaccga
     5941 atgcccttgc tgttgatagt gaacaacaga tggcagcagg tgacggcctt catggtcggc
     6001 taacgtttcg gcctgaccgc tgcgcactgt cgccaccagc gagttggttg tgcccaggtc
     6061 aataccagcc gccagacgac gctgatgcgg cgcagcactc aaaccaggtt cactaatttg
     6121 taataaggcc atgtttagct tccagaaatt aaaaatcgag cagtttttct tcgagttgtt
     6181 cggcactgct ccgcagttta tcgagaaaac gcagcttacg caccgtatcc gccgccgcgt
     6241 cccacgcctc gttgtctaac tgttcaacca tcaactgatg gcgggtatca aacatctttt
     6301 tcacacgttt gataaagctt tccagccgcg cttcatcttt cgcctgttcg atctcgtcca
     6361 gctcttcgcg cagctccaac tgttccatca ggaacgcggt gtcgcgcaca gtatgctgct
     6421 cgctggcgag atcaaaaccg tgtaaagaaa gcaaatattc cgcgcgcatt aacggatgac
     6481 gcagcgtttg ccaggcctgg ttaatggttg cagattgctg tacggcggcg agttgttccg
     6541 ccaggcttcc gctggcgaat ttatcaggat gatactgacg ttgtagatcc tgaaaacgca
     6601 ggctcagtgc ctgggtatcg agttgataac gggcaggcaa gccaaagagg gtgaagtaat
     6661 ccataacatt ctcagggctg cgatccgccg cgataacggc gaatcaggta aaacaaaccc
     6721 cacgcgcagg cgaccacggt ggggttatcg gtatgcgcat caaacgtgga agctttcgcc
     6781 gcaaccacac tcatctttaa cgttcgggtt ggtgaattta aacccttcat tcaggccttc
     6841 ttttacgaag tccagctgcg taccgtccag aaattgcagg cttttgccat cgaccacgac
     6901 tttcacgcct ttgtcttcaa acacgatgtc ttccggcgtc ggttcgtcaa caaattccag
     6961 tacataagcc atacctgaac atccggaggt tctcacgccc agacgcaggc caaacccttt
     7021 gccgcggtta gccagaaagg tatttactcg cgctgctgca ctgtcgctca gtgtaatcga
     7081 cataaccaaa cctcaactct tattttgctt cacgtttgct tttatagtcc gcaatggcgg
     7141 ctttgatcgc gtcttctgcc agaatagaac agtgaatttt caccggcggc agttcaagtt
     7201 cttcagcaat atcggtgttt ttgatcgcct gcgcttcgtc gagagacttc cctttcaccc
     7261 attcggtgac cagggagctg gaagcgatag cggaaccgca gccgtaagtt ttaaaacgcg
     7321 cgtcttcaat gataccttca tcgttgactt taatctgcaa cttcattacg tcgccacagg
     7381 ccggtgcacc caccatgccg ctgccgacgt tctcgtcgtt gttatcaaag gaacccacgt
     7441 tacgcggatt ctcgtaatgg tcgataactt tttcgctgta agccattata aattctcctg
     7501 attccgatac cgattaatga tgagcccatt cgatgctgtt cagatccacg ccctgcttgt
     7561 acatttccca cagcggagaa aggtcacgca gacgaccgat ggatttacga actaactcga
     7621 tggtgtagtc gatctcttct tcagtagtaa aacgacctaa agagaaacgg atagagctat
     7681 gtgccagctc gtcgttcagc cccagcgcgc gcagcacgta ggacggttcg aggcttgctg
     7741 acgtacaggc ggaacctgaa gaaactgcga ggtctttcag cgccataatc agcgactcac
     7801 cttcaacgta gttgaagctg acgttgagaa tgttcggcgc accgtgttcc aggtcaccgt
     7861 tcaggtaaac ttcttcgata tctttgatgc cgttccacag acggttacgc aggccgcgca
     7921 gacgttccat ctcggtcgcc atctcttctt ttgcgatgcg ataggcttcg cccattccga
     7981 cgatctggtg aacaggcaga gtgccggaac gcataccgcg ctcgtgaccg ccgccgtgca
     8041 tttgcgcttc gatgcgtaca cgcggtttac gacgcacata cagcgcaccg atacctttcg
     8101 ggccatagat tttgtgaccg gagaaagaca tcaggtcaac tttcaactgg ctcaggtcga
     8161 ttggctgttt gcccacgctc tgggttgcat caacgtgata gataatgcca cgagcacggc
     8221 acatttcgcc gatagccgcg atatcctgca ccacgccgat ttcgttattt acgtgcatga
     8281 tggaaacaag gatggtgtcg tcacgcatcg ccgcttcgag ttctttcagg tcgataatac
     8341 cgttacgctg cggtgccagg taggtgactt cgaaaccttc gcgctccagc tgacggcaag
     8401 tatccagtac cgctttgtgt tcggttttgc tggtgatgat gtgcttgcct tttttctgat
     8461 aaaagttggc tgcacctttg atcgccaggt tgtcagattc ggttgcacca gaggtaaaga
     8521 cgatttcacg cggatcagcg ccgaccagat cggcaatctg attacgggcg atatctaccg
     8581 cttcttcagc ctgccagccg aaacggtgag aacgagaggc cgggttacca aaggttccgt
     8641 ccatcgtcat gaactgcatc attttctcgg caacacgcgg gtccaccggc gtggttgcgg
     8701 agtagtcgag ataaatcggt aatttcattg ctctataaac tccgtacatc actcaatgca
     8761 aggaatcagg ctaccggctg gatgtacgac cgtgtttacg gagtatttag cactccggcc
     8821 tgattctgaa ttctttttat taagcgcgta acttaacgtc gatcgcgtct tgtgtgcggg
     8881 tgcgtggcgc gtcgtgagta tgctgacgac cagacacatc cagcacttcc tggttattaa
     8941 ccagttcgcc taaagtaatg ttgttgagaa aaccggtgag acggtcgctc aaatcacgcc
     9001 acagcgcgtg ggtcaggcat ttatcgccgc cctggcagcc gcctttaccc tgacaacggg
     9061 tggcatctac agattcgtca acggcgctaa tcacttcgcc aacggcgatg ctgctggcat
     9121 ctttgcctaa cagataacca ccgcctggtc cacgtacgct ggaaaccaga ccatttttac
     9181 gcagacggga aaacagttgt tccagataag aaagggaaat tccctgacgt tcggaaatat
     9241 cagccaacgg taccgggccc gcttcagagt tgagcgcaac gtcaagcatt gcggtcacgg
     9301 catagcgccc tttagatgtc agtctcatgt cttacttcac ctcaaactcg cccctgcccg
     9361 gggtttttta ttgtaaaagt gggggtattg catagcaggg tcaagtctga cattcccgag
     9421 taaattggtc aactatttac ttgactgatt tagtcgggta tttaaccttc agtgccattt
     9481 ttttatcgtg gcgtttgtac ctgttgtcgg atgcggtgtg aacaccttat ccgacctacg
     9541 attctgtaca ttgtaggcct gataagacgc gttaagcgtc gcatcaggca accactgtta
     9601 ttcggccttg ttacctttat tctgctgctc aatagaagcc agaatcccgc gcaggatatt
     9661 caattcctgg ctttccgggc gcgcacgggt aaacagacgg cgcaatttat tcatcacctg
     9721 ccccggatgg ttttcacgga taaaaccggt tgccagcagc gtttgttcca gatgaccata
     9781 aaaacgctcc agatcatcga ccagcggata cggcgtctct tcatgctcga cctgctcgcc
     9841 attttcctga gttgccagcc aggccatacg cacttcatag gcaataacct gaaccgccat
     9901 tgccaggttc agcgaactgt attccgggtt tgccgcaatc gcgacatgat aatggcattt
     9961 ctgcaactct tcattggtca agccgacgcg ctcacgacca aacaccagcg ccaccggggt
    10021 atttgccgct tcagcgacgc ttttcagacc gcattcgcgc gggtcgagca tcggccacgg
    10081 cagcgtgcgg gagcgtgcgc tggtgcccac caccaggcta caaccggcta acgcttcgtc
    10141 gagcgtatcg acgatgtgag cattaccaat cacatcgctg gcccctgcag ccagggcaat
    10201 cgcctgggag tcgggtttca ccagtggatt aaccagccac agattggtta atcccattgt
    10261 tttcatggca cgggcaacag aacccatatt gccggtgtgt gacgtctcca ccagcacaat
    10321 tcgaatattt tgcagcattg tctttcttca tctaaagatt attcacgcat cttatcataa
    10381 aacgaagaca gatgccgatc tcgctgctat actctgcgcc gttttcccgt tctttaacat
    10441 ccagtgagag agaccgatgc atccgatgct gaacatcgcc gtgcgcgcag cgcgcaaggc
    10501 gggtaattta attgccaaaa actatgaaac cccggacgct gtagaagcga gccagaaagg
    10561 cagtaacgat ttcgtgacca acgtagataa agctgccgaa gcggtgatta tcgacacgat
    10621 tcgtaaatct tacccacagc acaccatcat caccgaagaa agcggtgaac ttgaaggtac
    10681 tgatcaggat gttcaatggg ttatcgatcc actggatggt actaccaact ttatcaaacg
    10741 tctgccgcac ttcgcggtat ctatcgccgt tcgtatcaaa ggccgcaccg aagttgctgt
    10801 ggtatacgat cctatgcgta acgaactgtt caccgccact cgcggtcaag gcgcacagct
    10861 gaacggctac cgtctgcgcg gtagcaccgc tcgcgatctc gacggtacca ttctggcgac
    10921 cggcttcccg ttcaaagcaa aacagtacgc cactacctac atcaacatcg tcggcaagct
    10981 gttcaacgaa tgtgcagact tccgtcgtac cggttctgcg gcgctggatc tggcttacgt
    11041 cgctgcgggt cgtgttgacg gtttctttga aatcggtctg cgtccgtggg atttcgcggc
    11101 aggcgagctg ctggttcgtg aagcgggcgg catcgtcagc gacttcaccg gtggtcataa
    11161 ctatatgctg accggtaaca tcgttgctgg taacccgcgt gttgtgaaag ccatactggc
    11221 gaacatgcgt gacgagttaa gcgacgctct gaagcattaa tgactcaggc gggtgatatc
    11281 actcacccgc cctcgccttt caggcgctat tccgaaatac ttcctcaccg ctttactttc
    11341 tttcgtcact ctcccaccat ctttccccga ttaatggatg ataaagaagt cgcaaggaaa
    11401 ggttatggca ctgccagtga acaaacgcgt tcccaaaatt ctgtttattc tctttgttgt
    11461 tgccttctgc gtttatttag tgccgcgcgt tgccatcaac ttcttctatt atcctgacga
    11521 taaaatttac ggtcccgacc cctggtcggc ggaatccgtc gaatttacgg ctaaggacgg
    11581 tactcgtctg caaggctggt ttattccttc ttcgacgggc cctgctgaca acgccatcgc
    11641 aaccatcatt catgctcacg gcaatgccgg aaatatgtcc gcccactggc cgctggtcag
    11701 ttggttacct gagcgtaatt tcaacgtttt tatgtttgat tatcgcgggt ttggtaaatc
    11761 aaaaggcact ccgtcccagg ccggattgct ggacgatacg caaagcgcca tcaatgtggt
    11821 gcgccatcgc agtgatgtaa acccacaaca gctggtgctg ttcgggcaga gtattggcgg
    11881 ggcgaatatt cttgctgtta ttggtcaggg tgatcgtgaa ggcatacgtg cggtgatcct
    11941 cgactccaca tttgcctctt atgcaaccat cgccaaccag atgatccccg gcagtggcta
    12001 cttacttgat gagagttaca gcggcgaaaa ttacatcgcc agcgtcagcc cgatcccgct
    12061 tttactcatt cacggtaaag ccgatcacgt tatcccatgg cagcacagcg aaaagttgta
    12121 tagcctggca aaagagccaa aacggttgat cctaatcccg gatggcgaac acattgatgc
    12181 tttttccgat cgtcacggca atgtttatcg cgaacagatg gtggacttta tccttagcgc
    12241 gttgaatccg cagaactaac ccatgatcgc tagcacgata atcattcaca aaaccacctt
    12301 aagacatgct aatccactgg tcagaacagt ttaaaatgag aaaaattctg tgacgcttgc
    12361 caacatttct gatgattagc attcccttcg ccatttccct gagcaaactt tagctattct
    12421 tatcaattat gcttatggga gatctacaga tgatgcctac gcttgctcca ccatctgtcc
    12481 tttcggctcc ccagcgccgc tgccagatct tgctgacgct ctttcagccg gggttgaccg
    12541 ccaccatggc aaccttcagc gagcttaatg gtgtggatga tgatattgcc agtcttgata
    12601 tcagcgaaac aggacgggag atcctgcgct atcatcaact cacactgacg actggttatg
    12661 acggtagcta ccgggttgaa ggtacagtgc ttaaccaacg tttgtgttta tttcactggc
    12721 tacgacgtgg tttccgtctg tgtccgtcat ttattaccag ccaattcacc cccgccctga
    12781 agagtgaact gaagcggcgc ggaattgcgc gtaactttta cgacgatacc aatctacaag
    12841 cgttagtgaa tctctgctcc cgacggctgc aaaaacgctt tgaatcgcgc gatattcatt
    12901 tcctgtgtct gtatctgcaa tattgtttgc tgcagcacca cgctggaatt acgcctcagt
    12961 ttaatccgct ccaacgtcgc tgggcggaat cctgccttga atttcaggta gcgcaggaaa
    13021 ttggacgcca ctggcagcgt cgggcgctcc agcatgtacc gcctgatgag ccactgttta
    13081 tggcattact tttttccatg ttgcgggttc ccgatccact gcgggatgcg catcagcggg
    13141 acagacaatt gcgtcagtct atcaaacgtc tggtaaacca ttttcgtgag ctgggaaatg
    13201 ttcgttttta tgatgaacag gggttatgtg atcagcttta tatccacctc gcccaggcgt
    13261 taaatcgcag tttgtttgcc atcggtattg ataataccct gccggaagag ttcgccagac
    13321 tgtatccacg cctggtacgc accacccgcg cggcgctggc cggatttgaa agtgaatacg
    13381 gcgtccatct ttctgatgag gaaagtggtc tggtcgcggt gattttcggt gcctggctaa
    13441 tgcaggaaaa cgatctgcat gaaaaacaga ttattctact gaccgggaat gatagcgagc
    13501 gagaagcgca gattgagcag cagttacgcg aactaacgtt actgccgctc aacattaagc
    13561 atatgtcggt aaaggcattt ttgcagacag gcgctccgcg tggcgcggca ctgattattg
    13621 cgccttatac catgccgtta ccgctctttt caccaccgct gatctatacg gacctgacgc
    13681 tgacaacaca tcaacaggag cagatccgca aaatgctcga atcagcatga gggaacaact
    13741 ttcgggcgca aaaacattgc cggaagcgcc accagcgcca ttacccagaa cacgccgtgg
    13801 cccagatatt gatacaggaa accggcgaaa acggtcatga tagcgatact gccgcccatc
    13861 gcgacggcag agtaaaccgc ctgtaaacgg atgacttcgc taacctggcg agcagcaata
    13921 taacgcatgg cggccaggtg gcagaccgtg aaggtgccgc aatgcagaat ttgcaccact
    13981 atcaaccacg gcaacgccgt agttgctccc ataatgcccc agcgcactac gccgcaaatc
    14041 gccgagatca acagcatatc gcgagcacta caacggcgga aaagtttatt actcagcgca
    14101 aagataatga cttccgccac cacgcccagc gaccacaaat accccaccgc cgaggccgag
    14161 tagccagctg cctgccagta aatggcgcta aaaccgtaat aggccgcatg tgccccctgc
    14221 aataaacaaa cgcaggccag aaagcgccag ttctggcgaa ccagcgccaa ccacgcagac
    14281 caaccggtgc tctcctgctg gcggcttgcc ccttgtggct gaatcgtcgg acggatgaga
    14341 aagccgagca gcatggatgc cacgcccaac gtcaacagca cgaggatcac ccgataatca
    14401 aacatagtga ccagtttgcc cgtcagcgcc gagccaatga caaacgccac cgagccccac
    14461 agtcgcactt tgccgtaatc aagcgggaac tgcttttgcc acgtattcgc cagtgcatcg
    14521 gtcaacggta ccagcggtga gaaaaagagg ttaaagccaa tcatcaccag catcagccac
    14581 gctacgtgcg cccccgccca gaaggcgaca gcaaagagaa gtggcagcag tgccagcacg
    14641 cgcaaggcgg aaatcaggcg ggaaggatcg ctgacgcggg gcgcgatgag caaactcccg
    14701 agaaaacggg caaccagacc tgcccccaat aacaggccga tggtttctgg cgttaaacca
    14761 atccctttaa gccagacgct ccagaaaggt aaaaaaatgc cgtaactaaa aaagtatgtg
    14821 aaatagccga gcgccaacca gcgcgtggat tgcaaaacca tgagtccctc ccgtcaggag
    14881 gcgttagtct ggggttaatc gaatgttggt gcaagttgaa aaataagaat attcgagcct
    14941 gcttcccgat gtctgtaacg ccagttcaca agcactaaaa aactgcgatc cgcgacgcat
    15001 gttgcaaaaa taatgcgtaa ttatgccgtt acgcttgcca aacgttcctg cacaatggcg
    15061 atgaaatcgc gtaatgccag cttcatctcc cctttcttcc acgccatcag taaagcaatg
    15121 gaaggcacat ttccggcaat gggacgaaaa acaacctgcc cggtattaaa attattcata
    15181 taaccgggta tcaaagtgac gcccagcccc atgcccacca gattcatggt caccagaata
    15241 ttcgttgcca cctggacgat atttggctgg ctgttttctt gcgcaaacca cgctttaacg
    15301 atcggcgcaa gcgaaccgga atacgccgga tcggtactga cgaaattcac gccatccagt
    15361 tgcgcggcgg tgatctcttt ttcatgtgct aaagggtgat caaccggtaa caccaccact
    15421 aatggttcgt caaaaagctc cagataatca atctccgggc tataaacagg atggcgcatt
    15481 aagccgacgt cgagttcacc acggcgaatt ttttcctcct gttgcgtggt gattaaactc
    15541 accagctcaa tcaaggtgtc tggctgtctg agacgaaaca tcggtaatac ttttggcaat
    15601 aaattcactt ccgccgatgg cacaaagcca atggttaatt gtctgtcttc ctgaacaatt
    15661 ttccgcgccc gtaatttggc attttccgct tgttcgagga ttgccagcgc gtcctggaga
    15721 aaacattctc ccgccgccgt caacgcgact ttgcgcttat ccctcaccag taacggaaca
    15781 ccgacacagt tttcaagatc gcggatctgg ctgcttaacg aaggctgtga ggtatgcagt
    15841 ttttccgccg cacgggtaaa gttcagtgcc tgcgccactg cgacgaaata gcgtaaatgc
    15901 cgtagttcca tcaccttccc cttgttatcg aaaaaacgtc tcagctggta gaaattaact
    15961 atttcacata ttagcaacca accagcaaca tccttatggc acaaaaatag aaggtcaata
    16021 catcttatct ttcaggatta aaaaatatga ccacaccctc agatttgaac atttaccaac
    16081 tgattgacac ccaaaacggt cgggtcactc cgcgtattta taccgacccg gacatttacc
    16141 aactggagct tgagcgtatt ttcggtcgtt gctggctatt tctcgcccac gaaagccaga
    16201 tcccaaaacc cggtgatttc tttaacacct acatgggaga agatgcagtt gtcgtggtgc
    16261 gccagaaaga cggcagcatc aaggcgtttc tcaaccaatg ccgccaccgg gccatgcgtg
    16321 tgagttatgc agattgtggc aacagtcgcg cctttacctg cccgtatcac ggctggtctt
    16381 atggcattaa cggcgagttg atcgatgtac cgctggaacc tcgcgcctac ccacaagggt
    16441 tgtgtaaatc ccactggggg ctaaacgaag ttccttgtgt ggagagttat aaaggactga
    16501 tttttggcaa ctgggatacc agcgcaccgg gcctgcatga ttacctgggt gacattgcct
    16561 ggtatctgga tggcatgctg gatcgtcgcg aaggcggcac cgaaattgtc ggcggcgtac
    16621 aaaagtgggt gatcaactgt aactggaaat tcccggcaga gcagttcgcc agtgaccagt
    16681 atcatgctct gttcagccat gcttctgccg ttcaggtatt aggggcgaaa gatgatggca
    16741 gcgataagcg cctcggtgat ggacaaaccg cccgcccggt gtgggaaacc gccaaagatg
    16801 cgctgcaatt tggtcaggac ggtcacggta gcggtttctt ctttactgaa aaaccggatg
    16861 ctaatgtctg ggtcgatggc gcagtttcaa gctattaccg cgaaacctat gccgaagcag
    16921 aacaacgttt aggtgaagtt cgcgccctgc gcctggcggg tcataacaat atattcccca
    16981 cgctttcatg gctaaacggc actgccacac tccgcgtctg gcatccgcgc ggccctgatc
    17041 aagttgaagt gtgggcgttc tgtattactg acaaagccgc ctccgatgaa gttaaagccg
    17101 cttttgaaaa cagcgccact cgtgcttttg gtcctgctgg ttttctcgag caggatgact
    17161 cggagaactg gtgtgaaatc cagaaattgc ttaaaggcca ccgcgcccgc aacagcaaac
    17221 tgtgtctgga aatggggctt ggtcaggaaa agcgccgcga cgacggcatt cctggcatta
    17281 ctaactatat cttttcagaa actgccgctc gtggaatgta ccaacgctgg gccgatcttc
    17341 tgagtagcga aagctggcag gaagtgctcg ataaaaccgc cgcttaccag caggaggtga
    17401 tgaaatgagt gcgcaagttt cactggagtt acatcaccgc attagccagt ttctctttca
    17461 cgaagccagt ctgctggatg actggaaatt tcgtgactgg ttggcacagc tcgacgaaga
    17521 gattcgttac accatgcgca ccacggttaa cgcgcaaaca cgcgaccgtc gcaaaggcgt
    17581 ccagccgccg acgacctgga ttttcaatga caccaaagac cagctggagc ggcgaatcgc
    17641 ccgtctggaa acgggcatgg cctgggcaga agagccgccg tcacgcactc gtcacttaat
    17701 cagcaactgc caggtaagcg aaaccgacat cccaaacgta tttgccgtga gggtgaatta
    17761 tctactttat cgggcacaaa aagagcgcga tgaaacattc tatgttggaa cgcgtttcga
    17821 caaagttcgc cgtctggaag atgacaactg gcgcttgctg gaacgggata tcgtcctgga
    17881 tcaagcggtg atcacttccc ataacctgag tgtactgttc tgatgaatcg aatttatgcg
    17941 tgtcccgttg cggatgtgcc ggagggtgag gctctccgga tagatacctc gcccgtcatc
    18001 gccttgttca acgtgggcgg cgagttttat gccattaacg atcgttgcag ccatggtaat
    18061 gcgtcgatgt cagaagggta tctggaagat gacgccacgg tggagtgccc gctacacgcc
    18121 gccagttttt gcctgaaaac ggggaaagcg ttatgcctgc ccgccaccga tccgctcacc
    18181 acttatccag tacacgttga aggtggtgac attttcatcg acttaccgga ggcgcagcca
    18241 tgagcgatct gcataacgag tccattttta ttaccggcgg cggatcggga ttagggctgg
    18301 cgctggtcga gcgatttatc gaagaaggcg cgcaagttgc cacgctggaa ctgtcggcgg
    18361 caaaagtcgc cagtctgcgt cagcgatttg gcgaacatat tctggcggtg gaaggtaacg
    18421 tgacctgtta tgccgattat caacgcgcgg tcgatcagat cctgactcgt tccggcaagc
    18481 tggattgttt tatcggcaat gcaggcatct gggatcacaa tgcctcactg gttaatactc
    18541 ccgcagagac gctcgaaacc ggcttccacg agctgtttaa cgtcaacgta ctcggttacc
    18601 tgctgggcgc aaaagcctgc gctccggcgt taatcgccag tgaaggcagc atgattttca
    18661 cactgtcaaa tgccgcctgg tatcctggcg gcggtggccc gctgtacacc accagtaaac
    18721 atgccgcaac cggacttatt cgccaactgg cttatgaact ggcaccgaaa gtgcgggtga
    18781 atggcgtcgg cccgtgtggt atggccagcg acctgcgcgg cccacaggcg ctcgggcaaa
    18841 gtgaaacctc gataatgcag tctctgacgc cggagaaaat tgccgccatt ttaccgctgc
    18901 aatttttccc gcaaccggcg gattttacgg ggccgtatgt gatgttggca tcgcggcgca
    18961 ataatcgcgc attaagcggt gtgatgatca acgctgatgc gggtttagcg attcgcggca
    19021 ttcgccacgt agcggctggg ctggatcttt aaggaagcac gatgaaagaa aaaacgatca
    19081 ttattgtcgg tggcgggcaa gcggcggcaa tggctgcggc ctcgctacgc cagcaagggt
    19141 tcaccggtga gctgcatctg ttttccgatg agcgacatct tccttatgaa cgacctccac
    19201 tctcgaaatc catgttgctg gaagattccc cgcagttaca gcaggtgtta cccgctaact
    19261 ggtggcagga aaacaatgtt catctgcatt ccggtgtaac catcaaaacg ctgggtcgcg
    19321 acacacgaga gttagtgtta accaacggcg aaagctggca ctgggatcag ctttttatag
    19381 caaccggcgc ggcagctcga ccgctgccgt tgcttgatgc actgggagaa cgctgcttta
    19441 ccctacacca tgccggtgat gccgccagac tgcgagaagt tctgcagccc gaacggtcag
    19501 tcgtgattgt cggtgccgga actattggtc tggaactggc tgccagcgcc acgcagcgca
    19561 gcgcagcgca gcgcagatgt aaggtgacag tgattgaact ggcggcaacc gtcatgggcc
    19621 gtaatgcacc accgcccgtg caacgctatc ttttacagcg ccatcagcag gctggtgtgc
    19681 gcattctgct caataatgcc attgaacatg tggtcgatgg tgaaaaagta gaactgacgc
    19741 tgcaaagtgg ggaaacgctt caggctgatg tggtgattta cggtattggt atcagcgcca
    19801 acgagcaact ggctcgcgag gccaaccttg atactgccaa tggcattgtc attgatgagg
    19861 cttgccgcac ctgcgatccc gcgatctttg ccggtggcga tgtggcaatc actcgtcttg
    19921 ataatggtgc actacaccgc tgcgaaagct gggaaaacgc caataaccag gcgcaaattg
    19981 ccgctgccgc aatgttaggg ctaccgctac cgctactgcc gccgccgtgg ttctggagcg
    20041 atcagtacag tgataactta cagtttattg gcgatatgcg tggcgatgac tggctttgtc
    20101 gtggcaaccc ggaaactcag aaggcgattt ggtttaatct gcaaaacggc gtgcttatcg
    20161 gtgcggtcac gctgaatcag ggccgtgaga ttcgcccaat tcgcaaatgg atccagagcg
    20221 gcaaaacgtt tgatgcgaaa ctgctgatag atgagaacat cgcgcttaaa tcactgtaat
    20281 caggataatt agcgaatatc tcaatgcctg gggcgtggcg aggtgcaaga gtgtgtatta
    20341 cgtttaaatc acattatctt gcaaagggat tggttatgaa cacactacgt tattttgatt
    20401 ttggagctgc ccgccccgtt ttgttattaa ttgcccgtat cgccgtggtc ttaattttca
    20461 ttatttttgg ttttcccaaa atgatgggct ttgacggtac ggtccaatat atggcctcgt
    20521 tgggcgcgcc aatgccgatg ctggcagcga ttattgcggt agttatggaa gtgcccgccg
    20581 cgatattaat cgtgcttggc tttttcaccc gtccgctggc ggtgctgttt attttctaca
    20641 cgctgggtac ggcggtgatt ggtcaccatt actgggatat gaccggcgat gcggttgggc
    20701 caaatatgat taatttctgg aagaatgtca gtatcgctgg cgcgttcttg ctattggcaa
    20761 ttaccggacc gggggcaatt tctctcgatc ggcgttagga aagatgccgg atgcggcgtg
    20821 aacgccttat ccggcatttt ataaattaca gccactctac ccgcaacgac atctctgagg
    20881 ttgttgattc ccctggtgct agcgcaatga gatcaccccc ttccgggcga tgatgctcat
    20941 ccggcgcatg gctcatcggt tccagacaga agaaatcaaa cgcatatcct ttatcaaacg
    21001 cagggtcgga aacaaagatg aaataacacg gtgcaggtgg cgtcgtttcc atgatgatgg
    21061 cataaccttc ctgcggctgc tcgatgcggg cctgaccatt ccatccggca aaaccattgt
    21121 tcacccactg acgcggcaac ggcgcgagct ggttaaaatc cagttcctgc ggtagctgct
    21181 cgcaaaactc acccgccagc cactgctccc gttccagcca gtaaccgctc gcctgcgcct
    21241 gaatccgcgt ttgtggtgac aacggaaaat aaggatgcca gccggtacca aatggcagcg
    21301 tctctgcccc ttgattggtg acagagagcg tcaccgtcag cgtatccgcc gttaaatgaa
    21361 acgcctgact tacccgatag tgatagacac cgctgcgatg ttcatacacc agacacaaac
    21421 tatcatcgct gtgcgagaca cattgccact cgcccagcca gccatcgccg tgtagatagt
    21481 gcgcatccca ctcaacattc ggttgcagtt gatactcacg cccctgccag acaaaacggt
    21541 tgccgctcac ccggtttgca aatggcacca gcggaaaaca tgaggcatcg gttgccacac
    21601 cgcttttttt accaggacgt aagagcggcg tcgtatcgcg ccagaagcct tcgattacgc
    21661 cgccctggtc agaaacgtcc agctttagcg acccgtggga taaggtatag atggtcatcc
    21721 gctaactcct taatccggga agttaatcac aactttcccg catttgccgc tcgccatcag
    21781 cacataggca tcacctgcct gttctagcga gaagcgatgg gtaatggcgt tacgcggcca
    21841 cagcttccag tcagtcaaat cgtgggcgca tttttccata tggaacagac tggtcaccca
    21901 ggagccgata atccgccgtt gatggtgcat cagatcagcg ctgacctcga attccacttt
    21961 tccggtttca ccaatgtaaa ccacccgccc ccagtcagca gtggattgca gtgccagcaa
    22021 gcgacctgcg gcattaccgg aacaatcgag cgcaacatcc gcgccaccgt gggtaagttc
    22081 agcaattatc tgcggcagac cttcggtggt tgctaaatag ccgtgatcca tcccccctaa
    22141 ctgttttgcc atcgccagac gttccggcag catatcaacg ccgatgatcc gttttgcacc
    22201 gcgacctttc gccagcatca tcgccatcat gccgaccggc cccagaccga ccaccagcac
    22261 gttatcgctg ccggaaactt cgccgcgcaa aatcccttca taggccgtgc caacgccgca
    22321 actgataaac gcaccatctt cgtagctcag cgcatccggc aggaggatca gatctttttc
    22381 ttccgccagc agatattcgg catgaccgcc gtcacgctgc cagccgtaag ccgcttttcc
    22441 tttgccagta caagagatag gaaagccgcg gcggcagttc gggcaaaaac cacagccaga
    22501 aatgtgatac accagcacgc ggtcgccctc tttaaaatgg cggcagcctt gccccatcgc
    22561 cacaatctgc ccgcacggtt catgaccgtt gataaatccc tggtataacg gtttatcggg
    22621 tgccgccgcc gtggcacggt gttgatgata gatatagtgg acatcgcttc cgcaaatccc
    22681 ggaggatttc attttgatca gtacctggtt aatccccggc gtcggcaccg caacttcccg
    22741 cagatcgacg gttgaatttc ctagtaaata agcagccagc atcgttttca taaatccctc
    22801 attaacaata cgattaattt tcatccctgc agcacccgcg ccaggggcgt taaggtttag
    22861 cgtttcgctt tactgcttcg ctgggtcagc aagatattcg ccagcaccgc caccacgatg
    22921 atgacgccgc gtaccacctg ctggaaaaag gagttaatac cgagcagcac cagaccgtta
    22981 ccgattagcg taatcaccag cacaccaagc aatgtaccga acagggaacc gcgaccgccg
    23041 gaaagtgccg taccgccgac cacgaccgcg gcgatgacgt caaactccag accgtttgcg
    23101 gcacctgcgt taccggaacc gaggcgtgcc gccaacaaaa tgccggtcac cgccgctaat
    23161 aatcccgaaa gggtaaagat aagaatgcgc actcgacgaa cgttaatacc gcacaactgc
    23221 gccgccgtgg cattaccgcc aacggcaaaa accgagcgcc cgaaggcggt ttttcggcta
    23281 atgaacacaa acagcgcaaa caacaccatc atgatcagcg cggatactgg cacaccgaga
    23341 aattgtccgc ccagccagtc cagcacctcg ttttcgtcaa tcggcactgg cagtgcgttg
    23401 gtcataaaca gccccattcc gcgcagagcg ctccacagcc ccagtgtggc aacgaaactt
    23461 ggtacgttaa acacgccgcg cagcaccccg gcgagcgttc ccatcagcgc gcctaacagc
    23521 aacaccagca gacaagcaat cgccaacgga acttcaaatt gcagcaaaaa tgccaggcac
    23581 accgagacaa aagccaccat cggcccaacg ctgacatcaa tttcaccgga gataataatc
    23641 agcgtcatcg cccaggcggc aatcccaatg gtggcggcat cgcgcagcac gttcatctgg
    23701 ttattcaatg agataaagcc aggcgcgttc agggagaaga ccagataaag aatggcaatc
    23761 accaccagca aaccgatctc attaatatgg cgactgacaa attgtttgag cgagacgctc
    23821 ttgccctgcg gcaatggtaa tgacgaagca tacatcttca ggttcctcgg taaaattcat
    23881 cagtgcacag acagaatggc ggacatcagc tcatccacat tgaccggcga gtgaaactcc
    23941 tgcgagaacg tgccgtgctg taataacagg atgcggtcac acaccagcgg caattcctcc
    24001 acttcactgg agataaacac cacgcttttt ccttcggcag ccagctcacg gacaatacgg
    24061 taaatctgct gtttggcttc gatatcgacg ccgcgcgttg gctcgtcgag caacaaaatc
    24121 tggctggcgg catagaccca acgaccgatc accacttttt gctgattgcc accagaaagc
    24181 gtgccgatgg gtgtttcgct actggcagcc ttgaccgtca tccgctgcat cacctcttcg
    24241 gtcaggcggc ggatggtgga ccattgcagc acaccgttgg tgctgatttt ttgccgattg
    24301 gtcagcactg tattttcgtc aacgcctagc cagggaatga tccccgcttc tttgcggttt
    24361 tctggcgtat agccaatgcc gcgtttcagc atgtcgccgt aatcggggcg catgattttc
    24421 tcgccgttga taacaatttc gccctgttca tacgtctcca gcccaacaat cgccttcagc
    24481 aattcactgc gccctgcccc cagcaggcca gcaatgccaa gcacttcgcc acgacgtagc
    24541 gtaaagctga tatcctccag cttgggctta tggcgtaacg cacggacttc cagtacggcc
    24601 tgatccacaa tttcctgagg ggcaaccggc gcaatatcaa cgtgatcacg ccctagcatt
    24661 agcgacacaa tatgatgcgt ggaggtgttt tcgagcatca catcgcccgc cacctgacca
    24721 tcgcgcataa cggtggcata ggaggcaatg cggcggattt cttccatccg gtggctgaca
    24781 taaatcaccg ccacgcccag tgccgacatc tttttcaccg cactgatcac cagttcaact
    24841 tctgcactgg caagcgagct ggtgggttca tcaagaatga ccacgcgcgg ctcgcccttc
    24901 atcacccgtg caatttccac cagttgcttt tgcgccgggc ttagcgttga aacaagttgt
    24961 tcaggactaa cgtcaacgcc cagcgcctgt aagcaacgtt gggcatcctg cgccatttgc
    25021 aggtaatcaa tcatgccgtt gcggcgaggc cattgaccaa ggcagaggtt ttctgccacc
    25081 gtcagccctt ccaccagact taattcctga taaaccgcac gaacccccag ttcagcggca
    25141 cggcgagtca acgtagcttc gtcaccttcc agtcgcgtct cgccaatcca gatatcaccg
    25201 ctatccgggc gttcgctgcc ggtaagcatt cgaatgagag tcgatttgcc cgcgccgttt
    25261 ttacctaaca gcgcacgaac ttcgccttta ttgagcgtga agttaacgtt atccagcgca
    25321 acgacgccgg ggtaacgctt atttcctgcc accacttttg ctaccgggac tgcctctgtt
    25381 gccgtgaaca tagggacctc tgcgaatcag cgattagggc agaccatcaa cgtgcgttgc
    25441 cagccactgt ttaccatctt ccgttttggt atagagatcg attggcacct gaatcacttt
    25501 ttcaccgtcg gcttgtttat tgataacctt caatgtttgc gcgaaaacag cattgcccat
    25561 tttcttaccg gaaatatcca ctaccgcttt cagcacctga ttgttttcca gctcctgagc
    25621 aatttcggtt gtcatatccg aaccgaaaac agcaattttt ccggcctgat tttgattacg
    25681 taccgctttt accgcgccga gtgtcgcacc gcccgactcc cccataatgg cgttgagatc
    25741 cggcgtggag ataatcagtt tttcaccaac ggaaatcgct ttatctaaaa cagtcccttc
    25801 ctggttagcg acaatttgcg cgccgggaac gcgggatttt aatacttctt caaatccttt
    25861 acgtcgctgc acacaaactt caaaggcttc gcaattgatg acggcaattt tcggctggtc
    25921 aattttattg gcaataaaat aatcggcggc agcgttaccc agtttttacc aaattccagt
    25981 ggatcgccga ccagatacgc cgagacatat ttatcgacac ccttttgatt aatacaggtg
    26041 ttgtagcaaa tcaccggaat acccgcttca ctggcgcgac gaacggtacg gctactgcca
    26101 ttttcagaca ctgccgataa aataatggca tcgacattac gcgccacgag ggtatcaaca
    26161 aaggtacttt ctttcgaaat atccccctgg gcgttagttt caattaactg tacctgtact
    26221 gaagaatctt ttgccgcatc ctgaacgccc tgacgcactc cagcgtaata tccctgggta
    26281 tcaaggtata ttgcaccaat ggtcatttct ttatccgcag ccctggcaaa tagtgcgcta
    26341 cctaatagcg tagccattaa taatagatta cgggttgttc tcattttttt aggcatagtg
    26401 cctcctgtag ggtttttatt aacaacggct tattctaatt attttgtgat gagcggcagc
    26461 gcgtgccgct caaatattac agttaatagg gtttagtgaa cgtagttaaa aataaaaggc
    26521 atcacagtgg tgaataatgc cgctttcggc caggccggat taatcgccgt caattcagcg
    26581 cgagcagact catattgcgc aacctccccg agtcccgcgg acgccagcat cgccaccatc
    26641 aggcattttt ctttatgctg ctgttgtaaa tcgccataca gcgataacag gtcaggttgt
    26701 gaaacagcaa agaaatccgc ctcaatactg gttttcgcca tctcttgcgc ccactgtttc
    26761 atttcactaa acagttgctg tgcggtttgc tgttcaccca gcagtcgcag cgccattcct
    26821 tgccagaaga gataatcaac cggctgatcg ttgtaataac tgtggatgtt aatggtgcga
    26881 tcgccggtcg ccgccagacg taaacaacgc atcgcttcag tttcatcgcc ctgcgcgttg
    26941 gcgcatatcg cctgccagaa ccagatgtcg ttatcagttt gccccggtaa acggccttcg
    27001 cttaaattct ccggataata cagcgcggca tgaagcagtt cgctggcctg ctgcggctct
    27061 ctggcatcaa gatgctgcca ggcgcgtaat aactggttga ggataaactg actggtgacc
    27121 ttcccttccc cgccttccca cgggtggaat ttacgcgtgg cgagaatgtc cgccgctttg
    27181 tctgcctgac ccgtgagatg ccacaaattg agcagttctg cggtcatgtc gtcgcgtttc
    27241 agcgcaattt ccagattatt ttccagacgc gccagtcgtt tctccggtgt ggctccactt
    27301 aacttatcca gcaaatcccg ttcgaaaagc agacgtgcat cctgcggcgc aagctgataa
    27361 gcattatcaa gataacgcgc ggccagctca taatcgtgtt tcttattcca cgcatggatc
    27421 gctaacccgc gccagccgtc ggcaaactcc ggcgacatct ctacgcaacg ttgccataag
    27481 gcaatggctt tgttgtagct acgtttgttg tagtagaagc aggccagtaa atggcgagca
    27541 aaccaacact cttcaatact ctccagcgcc gccacttctt ccagcgtatt cgggaaacgg
    27601 acaaactgcg ggaagacatc aatggctttt gcgaccagtt cgccacgttc ggctttcggc
    27661 agcaagctgg cttgcaggta aagcggcagc gtgcgctggc agtccagtgc gttcagcatc
    27721 tctgccgcca gggtgggcat tccccagtta atcagttgcc cggcggtcat cagagcgtta
    27781 acgtcgcgtc cctgacacag accgcgccac tgcgcgaggg ctgattcgct acgaccatcg
    27841 aaccagttca gccaccacag agtggcgttc agcggataat cacgcagcag tttctcgcgc
    27901 tgcaaacgcg cgttgtcctg acgaccactt aacaccagca gcagattatg caagcaaagc
    27961 acttcctgat tggttgggca ggcacgaaga ctttgttggc aaaaatccag acccgcgtcg
    28021 aagttatcat tacgcgccgc cagtcgtgcc agaccataat agccgcctgc tttactattg
    28081 ccgctccaga ccgcacgcca gaaatcctct tcggcttgtt gatattgtcc ctgacgttcg
    28141 taagcactgg cgcgaatcaa acttgcttgt ccacactgcg gttttttgtt cagcgcatgt
    28201 gcacgtttca gcgcctgact ggcatacgcc accgcttgcg ggaaatctgc gcggttatat
    28261 tccagcatcg ccagcgccag gttacagcga taatccagcg ggtccagcgc cacgccgcgc
    28321 aggtagtaat cgaacggtga acggctggcg tgatgatatt gctccagatg ctgaccgata
    28381 aaccaggctt catctgtact ggtaatgtct tgtgctgcca gtggcgcttt ggcgacgtcc
    28441 ggcaacggca actcctgcgg ctgatgttcc tgataactca gtacaatatt gccgtcggca
    28501 tcggagagtt caatggtcag tctgtcagga ttgataccgt gcaacacgcc ctggatggcg
    28561 gtcgctggtg tcagggccac ggcatcatcg agcaacgcgt tgcatttgcc gatttcgcgg
    28621 atcgccagac ggtatccgtt caacggagag atggcataca gcccccactc aatcccccgc
    28681 tcactacgct ggagttttat caccgcatcg cgggaggcat tttgcaccat gcccagagaa
    28741 tgataaggca ggaaatactg ctcgaaacgc ttctcttcgt aagcatcaag ccaggtaaaa
    28801 tcaggctggt tatcggcaaa aataccggtc atcagttcga tatacgggcc gttattgtcg
    28861 gtcagactct tatcccacgc ctggccaaat tcactgtgtc cccaactcca ctgtttttta
    28921 cctggcgcaa tatggtggtt ggcaacgtgc agcaaaccgc catcttcatc gtgacaccac
    28981 gcgccgacaa aatcgtactg cgatttttcg gccatatatg aggttggaac aggcacattt
    29041 ttatagcgag aaatgtccac tccggcggag tagtccactt tgtagtaagt gccggtggcg
    29101 atggggaaag cggagacggc ccgtttgccg tgatcaaaca ccgcagtcac atccggtggg
    29161 aaaacgctct gatgcccttc accccctttc actgccgggt tgacccacca caagaaatga
    29221 cgcggcgtgg cgttgccgtt atagacgcgg ctggcgattt ccagtgccgc ccggtcaggg
    29281 cgcagggtga aacctgtcat cacctgtaaa ccatgcatcg gctccgtttc gccgacccac
    29341 accgtctgtg caccgtcttc atgggcttcg agggtgaaat caacgggcat aaaggtggtc
    29401 gggcgatggt gttgcggcca gttaaactca atcccgccgg agatccacgg tcccagcatc
    29461 cccaccagcg caggtttaat gacttcattg tgatagacaa aatcgcgctg tttcacttta
    29521 tcccatgcgc gatgcacccg accgcccagt tccggcagga tcatcacttt gatgtagtcg
    29581 ttttccagcc acaccgcctg ctaggatttc agggttttct gctcgctcag cgtatcggtc
    29641 acgccgtagg gataaaccgc gccggacgat ccctgataaa cgcgattttc caggaacatg
    29701 ggatgtatat cctgcggccc ggtttcatag gtcgggatct caacgcgctc ttgccacact
    29761 tttactggag tcatggtgcc ctcaatatta acaagacata ctgaattaaa agatttgtgg
    29821 cagtgtattg aacaatctgg caatgttttc gcggaataat cacgcaatta actaaacaag
    29881 gtttagtgaa gatgagagcc tgcattaaca atcaacagat tcgccaccat aacaaatgcg
    29941 tgattctgga actgctgtac cggcaaaagc gcgccaataa atcaacgctg gcccggctgg
    30001 cgcaaatttc gattccggca gtcagtaata ttttgcagga actggaaagc gaaaaacggg
    30061 tggtgaatat tgacgatgaa agccagacgc gcgggcatag tagcggtaca tggctgattg
    30121 cgccggaagg tgactggacg ctgtgcctga acgtgacgcc caccagtatt gagtgtcagg
    30181 ttgctaatgc ttgtttaagt ccgaaaggcg aatttgagta tttacagatt gatgtaccga
    30241 cgccgcaggc gctgctgtcc gaaatcgaaa aatgctggca tcgccaccgt aaattgtggc
    30301 cggaccgcac catcaatctg gcgctggcaa tccacgggca ggttgatccg gtgaccggcg
    30361 tatcgcaaac catgccgcaa gcgccgtgga caacgccagt tgaggtgaag tatctgctgg
    30421 aagagaagct gggcattcgg gtgctggtcg ataatgactg cgtgatgctg gcgctggcgg
    30481 agaaatggca aaataattcg caggaacggg atttctgcgt gatcaacgtt gattacggca
    30541 ttggctcgtc gttcgtgatt aacgagcaaa tttatcgcgg cagtttgtat ggtagcggac
    30601 agattggtca caccatcgtt aatccggatg gagtcgtctg cgactgtgga cgttacggca
    30661 gcctggaaac cgtcgcctcg ttaagtgcat taaaaaaaca ggcgcgggta tggctaaaat
    30721 cacaaccggt taatactcag cttgagcctg aaaaactgac aaccgcgcag ttaatcgctg
    30781 cctggcagag tggagaaccg tggatcacca gctgggttga tcgctctgcc aatgccattg
    30841 gtttgagtct gtataacttc ctcaacatcc tcaatattaa tcagatttgg ttgtacggtc
    30901 gcagttgtgc ctttggtgag aactggctta atactattat tcgccagaca ggatttaacc
    30961 cgttcgaccg cgacgaagga ccgagcgtga aagcgacgca aattggcttc gggcaattaa
    31021 gccgcgcaca acaggtgctg ggaattggct atttgtatgt tgaggcgcag ttacgacaga
    31081 tttgatggcg cgataacgta gaaaggcttc ccgaaggaag ccttgatgat cataaacgaa
    31141 aaattgcctg atgcgctacg cttatcaggc ctacacggag attgcaatat attgaatttg
    31201 caagattttg taggcctgag aaggtgttca tgccgcatcc aacatgaaca acgagcacat
    31261 tgtcagcaaa tcaccgtttc gcttatgcgt aaaccgggta acgtgcgcag atgtcgagaa
    31321 ctttaccttt gatgcgctcg ataacggctt catcattgat gctgtccagc acgtcacaca
    31381 tccagccagc cagttctttc gcttcggctt ctttaaagcc gcgacgggta atcgccggag
    31441 tacctacacg aataccggag gtcacaaacg ggctcttcgg atcgttcggt acgctgtttt
    31501 tgttgacggt gatgttagca cggcccagag cggcgtctgc ttctttaccg gtcaggtttt
    31561 tatcaaccag atcaaccagg aacaggtggt tatcagtgcc gccggaaacc actttgtagc
    31621 cgcgctcgag gaacacttct accatcgctt tagcgttttt cgcgacctgc tgctggtaag
    31681 ttttgaactc aggctccatc gcttctttca gagcaaccgc tttaccggcg attacgtgca
    31741 tcaacggacc gccctgacca ccagggaaaa cggcagagtt cagttttttg tacagctctt
    31801 cgctaccacc tttcgccaag atcaggccgc cgcgcggacc cgccagggtt ttgtgagtgg
    31861 tggtagtaac aacgtgagca tgaggaaccg ggttcgggta gacgccagca gcaaccaggc
    31921 ccgcaacgtg cgccatatca acgaacagat aagcaccgat gctgtcagcg atttcacgca
    31981 ttttcgccca gtcaaccacg ccggaatatg cagagaaacc accgataatc attttcggct
    32041 tgtgttcttt ggcttgtttt tccagatcgg cgtaatcgat atgaccggta gcatcgatac
    32101 cgtaaggaac gatgttgtac agtttaccgg agaagttaac cggagaaccg tgagtcaggt
    32161 gaccgccatg cgccaggttc atacccagaa cggtatcacc tggttccagc agcgcggtgt
    32221 agaccgcaaa gttagcctgg gagccggagt gcggctggac gttagcgtag tcagcgccga
    32281 acagttcttt cgcacgatcg atcgccagtt gttcaacgat atcaacatac tcgcaaccgc
    32341 cgtagtagcg tttgcccgga taaccttcag catatttgtt ggtcagctga gaaccctgcg
    32401 cctgcattac gcgcgggctg gtgtagtttt cggaggcgat cagttcgatg tgctcttcct
    32461 gacgtacttt ttcctgctcc atagcctgcc acagttcggc atcataatcg gcaatgttca
    32521 tttcacgctt taacatccgc atctcctgac tcagctaaca ataaaatttt tggcctttat
    32581 aggcggtcct gttggacaac ggcgaacagt ataaccgaat cattgtgcga taacaggtct
    32641 tgacaaagga atttacgcaa acgattacct tcaggctacg caaggctttg gagaataaag
    32701 agcttgcaac cggaaacgga tttcttttca ggtttgtgat gcaaattttt cacttcatca
    32761 cattctttct gaaaaacacc aaagaaccat ttacattgca gggctatttt ttataagatg
    32821 catttgagat acatcaatta agatgcaaaa aaggaagacc atatgcttga cgctcaaacc
    32881 atcgctacag taaaagccac catcccttta ctggtggaaa cggggccaaa gttaaccgcc
    32941 catttctacg accgtatgtt tactcataac ccagaactca aagaaatttt taacatgagt
    33001 aaccagcgta atggcgatca acgtgaagcc ctgtttaacg ctattgccgc ctacgccagt
    33061 aatattgaaa acctgcctgc gctgctgcca gcggtagaaa aaatcgcgca gaagcacacc
    33121 agcttccaga tcaaaccgga acagtacaac atcgtcggtg aacacctgtt ggcaacgctg
    33181 gacgaaatgt tcagcccggg gcaggaagtg ctggacgcgt ggggtaaagc ctatggtgta
    33241 ctggctaatg tatttatcaa tcgcgaggcg gaaatctata acgaaaacgc cagcaaagcc
    33301 ggtggttggg aaggtactcg cgatttccgc attgtggcta aaacaccgcg cagcgcgctt
    33361 atcaccagct tcgaactgga gccggtcgac ggtggcgcag tggcagaata ccgtccgggg
    33421 caatatctcg gcgtctggct gaagccggaa ggtttcccgc atcaggaaat tcgtcagtac
    33481 tctttgactc gcaaaccgga tggcaaaggc tatcgtatcg cggtgaaacg cgaagagggt
    33541 gggcaggtat ccaactggtt gcacaatcac gccaatgttg gcgatgtcgt gaaactggtc
    33601 gctccggcag gtgatttctt tatggctgtc gcagatgaca caccagtgac gttaatctct
    33661 accggtgttg gtcaaacgcc aatgctggca atgctcgaca cgctggcaaa agcaggccac
    33721 acagcacaag tgaactggtt ccatgcggca gaaaatggcg aagttcacgc ctttgctgat
    33781 gaagttaagg aactggggca gtcactgccg cgctttaccg cgcacacctg gtatcgtcag
    33841 ccgagcgaag ccgatcgcgc taaaggtcag tttgatagcg aaggtctgat ggatttgagc
    33901 aaactggaag gtgcgttcag cgatccgaca atgcagttct atctctgcgg cccggttggc
    33961 ttcatgcagt ttaccgcgaa acagttagtg gatctgggcg tgaagcagga aaacattcat
    34021 tacgaatgct ttggcccgca taaggtgctg taatttgatg ttgccggatg gaaacatccg
    34081 gcaacccttg acgcggttta aattgccgcg tcgtcctctt caccggtacg gatgcgaatg
    34141 acccgagcca cgtcaaagac gaagatttta ccgtcaccga ttttgccggt ttgcgccgtg
    34201 cgaataatgg tatcgacaca ggtatcgaca atgtcgtccg gtacgacaat ctcaattttc
    34261 actttcggca gaaaatccac catatactcc gcgccgcggt acagctcggt atggcctttc
    34321 tggcgaccaa agcctttcac ttcggtcacc gtcatgccgg taataccgac ttcggccagt
    34381 gcttcgcgga catcgtccag cttgaagggt tttataatcg catcaatctt tttcatgcta
    34441 ttccttgaaa aggttgcctg tcttttgatc tgctaaacgt aacacataac gccaattcat
    34501 tccttgaaat cgtttgcatc cagctcgtgt cgggaaagca gtttataaaa ttctgtccgg
    34561 ttgcgccccg ccattctcgc cgcgtgggtg acgttgcctt tggtgatttg cagcagctta
    34621 cgcaagtagt tgagttcaaa atgattacgt gcctcaacaa aggttggcag cgccgtattt
    34681 tcaccctcca gcgcctgctc caccagcgca tcactaatca ccggagatga ggtcagcgcc
    34741 acgcactgtt caatcacgtt gaccaactgg cgcacattac ccggccagct cgcggtcatc
    34801 aggcgtttca tcgcatcggt agagaacgcg cggacaaacg gtttatgtcg ctctgccgcc
    34861 tggcgcaaca ggtgatttgc cagtagcgga atgtcttctg tgcgctccgc cagtgccgga
    34921 attttcaggc tgacaacgtt gaggcggtaa tagagatctt cgcggaattc cccgcgcgcc
    34981 atcgcttttg acagatcacg gtgagtggca gaaataatcc gcacattgat atcaatatcg
    35041 cggttactgc ccagcgggcg cactttacgc tcctgcaaca cgcgcagcag tttgacctgt
    35101 aacggcgcag gcatatcgcc aatttcatcg agaaatagcg ttccgccttc cgccgcctgg
    35161 aataaacctt cgcgattgct gacagcgcca gtaaacgcgc cacgcgcatg accaaacagc
    35221 tccgactcca gcaattgctc gggcaatgcg ccacagttaa tagcaataaa tggtttgctg
    35281 ttgcgcgggc tggcgttgtg gatagcctgg gcgaaaatct ctttcccggt gccgctctga
    35341 ccgttaatca aaacgctgac gtctgattgc gccaccagcc gcgcctgttc cagcaaacgc
    35401 agcatcagcg ggctgcgggt gacaattgcc tcgcgccagc gttcatcggt ggctggcgcg
    35461 gattgctcca gcgcatcgtc aattgcctga tatagcgcat ctttatcgac aggcttggtg
    35521 aggaaactaa aaacgccctg ctgtgttgca gcaacggcat cggggataga accatgcgca
    35581 gtaagaataa ttactggcat tcccggctgc actttctgga tttcagcaaa tagctgcata
    35641 ccgtccattt catccatccg caggtcgctg ataactaaat ctactttttc gcgattcagt
    35701 acccgtaatc cttcagcgcc actttccgcc gtgaccacac tgtagccttc gctggtcagg
    35761 cgcaggccaa gcagtttcag caatcccgga tcgtcatcga ccaataataa atgcgcaggt
    35821 ttatggctca tcaggagtga cctcatgggt ggatggcgcg ggcttttcac tctcgtgcgg
    35881 cgtatccggc gagaaatttc cagccggttt gcgggtcgag agctggcgtt caatatcggt
    35941 caggttttcc agcttgcggg tggtgagttc cagttgctgc tgtagaacgt gatgttgctg
    36001 gcgcaatgta tccagctcgc tgtcgctgga ctgctggagt ttactgtaac gttggcgctc
    36061 ttccgccagt tgcagttgca gcgcctgacc gtcgcgccag agttgataca gtgggcgaac
    36121 ctgtgccggg atctcggtac ttaacgcttc aatacgcgcg accagctggc ggcgctcata
    36181 cggcgtaatt ttggcgtcgg cgagcaaaat cccttgttta aaggtatttt gccagctgcc
    36241 gtcgtcatat tggcgagctt gctgacgcga ctgcgcaggc attaaacgat cagcacaatc
    36301 catcgcccgc agccagtaaa gcggattggt ttcggttgat ttaccttgca gcgcccagat
    36361 gtcgctacat tcagtagaaa gatagtcagc cagttgataa accggaattt tttcttctgc
    36421 tggcgtatca atggctggct tattgtgatt ctgcacgcaa cccagcaatg ccagacatgg
    36481 cagccctgcc agccacagcc gtcggggcaa taatcgttga aaaatgtgtc gcatattcac
    36541 cagacttaaa gcctatccca gtgggcgtaa ttgttgcaga cagtctggac atggacagcg
    36601 cggagaaacc ggggcgtaca tatcgtacgt gaagatttcg agcactaccc ggggccgaac
    36661 tgacaaataa aatagcctga taggataggc tgttagcatt atttcgtgtt tttcgacgac
    36721 ggtaattcaa tgcggaaaca aacgtcttgc ccgctctcgt cgaccagata cagttcccct
    36781 tgcatacggc gaatacaatc cctggcaatg cttaatccca gaccgctgcc cttcaccgcc
    36841 ccttttcgct ggtggcttcc ctgaaaaaag ggttcgaaga tcatggcgcg ttcctcttgc
    36901 ggaatgggcg tgcctgtatt gatgacatca atataaaccc gcgcaccatg taaactgctg
    36961 cgaaggcaaa tgttaccgga ttcagccccg tagtgcaccg cattggagta aagattatcc
    37021 agtacgctca tcagcagcat tggctccgcc aggcaagctg ttgctttgag atcgacgtcg
    37081 gtatgcatca ttttagcccg tgcgggcagg ctatgagcag aaaccactgt ctccaccagc
    37141 ggtgctaatt caacattctc cagttccacc gcactgtccg cctgtttacg gttgtaatca
    37201 agcagttgtt cgatcagttt ttgcaaattg cagctgctgc tatcaagaat gctcaccacc
    37261 tctttttgct ctggcgtaag cggcccgaca acctgatcag ccagtaattc agtgccctcg
    37321 cgcatactcg ccagtggcgt ttttaattca tgagataaat gtcttaaaaa ttgatggcgt
    37381 tgggattcca gccatgacag gcgctcactt aaccaaagaa tacgttgccc aaccgagcgt
    37441 aactcgctcg gtccacagaa cgaaacgcta ttgcccagag aacgcccttc ccccagacgg
    37501 ttgatcatgc gctcgatatt tttcaccggc ccgataatca tccgcgtgaa aagtagcacc
    37561 atcaccagac tcaccagaaa tagcaccagc gattgccaac caaaatattg cccacgttcg
    37621 gcgatttcac gctgaagttg ctgcccacga gagaacacca ctgtgcgcgt ggcctgcacc
    37681 atttcggtat tggcactggc aaaggcttca agacgcgcgg cggcggcagc atcgggacca
    37741 ctgttgttac actgaagttg ggccagattg ttcaaatcct gacgcaatgc ctggtaaagt
    37801 ttatcgtctg gcagtacgcc tgcgtgggca tcgagcattt cgctgtaacg cttgcgctgg
    37861 ctttgataaa ccttcgcaag cgtagggtcg tccagcacgc aatactgacg gtagctacgc
    37921 tccatctcca gcgccgcgtt ggtcatcgct tcactacgcc gggcatcaat aagcgtagtg
    37981 cggttaacca gcgccgcctg atcgctaagc gcattcaggc tttgccaggc ttgccatgcc
    38041 agcaccaaca ggggtagtag aattagcaaa aatgccagca ttaccagttg tcgtaatgag
    38101 cggggaaaaa cgggccagcg tttcaaggtg ttactctcgt cagacgcgaa tagcctgatg
    38161 ctaaccgagg ggaagttcag atacaacaaa gccgggaatt acccggcttt gttatggaat
    38221 aaggcggtgc ctaactcgac gtttcgcccg atggttgata tagctacgct gatatcagaa
    38281 gttggacggc aggcaccttg ttgtgcgtca ttcgaatttt atgtagcacg tcccgaaggg
    38341 gctgacataa gtcggtgaat gagccactgc ttactattat gcagtaactg tgccaataaa
    38401 gaaaatagtt tggtaacgta ctgattatac gttgctttga gggatttatg gcttcttcgc
    38461 tatttgaata taaatcagcc actgtgtcgc taaaaagaga caacttaaga taaacttatt
    38521 agattgcaat aaaatcaatc atttaagtgt cgccagaaag cgacacggta aaccacccat
    38581 tgtcgtgatt tacagacaca aaaaagctcc cggagttggg agcttatgat agtggttggt
    38641 gcttaatgcc gcatccgggc tgcaaaacca atgaactgat gacttacccc aactgcttac
    38701 gcgcattgcg gaaaatgcgc atccatgggc catcctcgcc ccagttttcc ggatgccagg
    38761 agttgctgac agtacggaaa acacgttccg ggtgcggcat cataatggtg actcgaccac
    38821 tttcagtcgt aaccgccgta ataccgttcg gtgaaccgtt cgggttagcc gggtaggttt
    38881 cagtgacttt gccgaagtta tcgacatagc gcagtgccac cagccctttg ctttccagtg
    38941 ccgccagatg cgccgcatca cgcacttcca cgcgcccttc accatgagag acagcaatcg
    39001 gcatttgcga gcccaccatc ccctgcaaca gcagagacgg gctttgggtt acttcaacca
    39061 ggctgaaacg cgcttcaaag cgatcggagg tgttacgcac aaaacgtggc cacaactcac
    39121 tccccgggat cagttcacgc aggttagaca tcatctggca accgttacat acccccaggg
    39181 ccagcgtttg cggacgatgg aagaaggttg caaactcatc gcgtacacgg tcattgaaca
    39241 ggatagactt cgcccaacct tcaccggcac ccagcacatc accgtaggag aaacctccgc
    39301 acgcgaccag ggcgtgaaaa tcctccagtc ccgtacgtcc agtcagcagg tcgctcatat
    39361 gcacgtcgat agcatcaaag cccccacgtt ggaaagctgc cgccatttca acatgcgagt
    39421 tcacgccctg ctcacgcagt acggcaactt tcggacgtgc gccagtggca atatacggtg
    39481 ctgccacatc ttcgttgata tcgaacgaca gttttacatt caggcccgga tcggcgtcgt
    39541 tagatttcgc ctgatgctcc tgatcggcac actccgggtt gtcacgcagg cgctgcatct
    39601 gccaggtagt ttctgcccac cagacacgca acgtagtgcg gctttcgctg aatacagtct
    39661 gcccgttggc ggtaatcaca aaacggtcac cggaaaccgc ctgcccaaca taatggacgc
    39721 aatccgcaag cccatgctgt gccagtacgg actcgaccgc ttcacggtca gcggcacgaa
    39781 cctgaatcac cgcacccagt tcttcgttaa acaacgccgc caggcgatcg tcacccagag
    39841 aggcgatatc cgcgtcaatg ccacaatgac ctgcaaaggc catttccgcc agcgttacca
    39901 gcaggcctcc atcagagcgg tcgtgatacg ccagcagctt acgctgtgca accagcgcct
    39961 gaatcgcgtc atagaagcct ttcagttgga caacatcgcg tacatctgcc ggtttgtcgc
    40021 caagctgacg ataaacctgc gccagcgccg ttgcgcccag cgcgttattg cctttgccca
    40081 aatcaatcag cagcagtgcg ttatcttcgg tacaaagctg cggcgtgatg gtgtgacgta
    40141 catcttccac gcgggcaaat gcagaaatca ccagcgacag cggcgacgtc atttcgcgct
    40201 cttcgttacc ttcctgccag cgggttttca tcgacataga gtctttaccc accgggatcg
    40261 tcaggcccag cgccggacaa agttcttcac ccacggcttt aacggcttca tacaggcccg
    40321 catcttcacc agggtggcct gccgccgcca tccagttggc ggaaagttta atgcgtttga
    40381 tatcgccaat ctgtgttgcg gcaatattgg ttaacgcttc accgaccgcc agacgggcag
    40441 aggcggcgaa atccagcagc gcaaccggcg cacgctcgcc aatcgccatc gcttcaccgt
    40501 agtagctgtc gaggctggcg gtagtgaccg cgcagttagc gaccggcacc tgccacggcc
    40561 ccaccatctg atcgcgcgcc accatgccgg ttacgctgcg gtcaccgatg gtcaccagga
    40621 aagttttctc cgccacggtc ggcagatgca gtacgcgttt caccgcgtct gcaatggtga
    40681 tcccttcacg gaccagcgca tcgcccttcg ctttcagcgt ttgtacatcg cgggtcatct
    40741 tcggcgtttt accaagcagg acgtccagcg gcagatcgat cggctgatta tcaaaatgac
    40801 gatcgtgcag agaaagatgc agttcttcgg tcgcttcacc aatcaccgcg tagggtgcgc
    40861 gctcacgctt acacagttcg tcaaacagcg gtaattgatc ggcagcaacc gccagcacgt
    40921 agcgttcctg ggattcgtta caccagattt ccagcgggct catgcccggt tcgtcgctta
    40981 gaatatcgcg cagttcaaat ttaccgccgc gtccgccgtc gctcaccagc tccggcatcg
    41041 cgttagaaag accgcccgca ccgacgtcgt ggataaacag aattgggtta gcatcaccaa
    41101 gctgccagca acggtcgatc acttcctggc aacgacgctc catctccggg ttgtcgcgct
    41161 gtacggaagc aaagtcgagg tcggcatcag actgaccaga cgccatggaa gacgccgcgc
    41221 cgccgccaag accgatgttc attgccgggc cgccgagaac gaccagcttc gcaccgacgt
    41281 tgatctcgcc tttttgtacg tgatcggcgc gaatgttgcc gatcccgccc gccagcatga
    41341 tcggtttgtg ataaccgcgc agctcttcgc cattgtggct gttcactttt tcttcataag
    41401 tacggaagta gccgttcagt gccggacgac caaattcgtt gttaaacgcc gcaccgccca
    41461 gcgggccttc ggtcatgatg tccagcgcgg taacaatgcg ctcaggctta ccgaaatctt
    41521 cttcccacgg ctgttcgaag ccaggaattc gcaggttgga tacggagaaa ccaaccaaac
    41581 cagctttcgg cttcgcgccg cgcccggtgg caccttcatc gcggatttca ccgccggaac
    41641 cggtcgccgc gcccggccac ggagaaatcg ccgtcgggtg gttgtgagtt tcgactttca
    41701 tcaggatgtg cgccggttcc tgatggaaat tgtagcggcc cgtttcgtgg tcagcaaagt
    41761 agcggcccac ttcagaacct tccattacgg cggcgttatc tttataagca gagagaacgt
    41821 gatctggcgt ggtttcgaaa gtatttttga tcatcctgaa cagcgatttc ggctgttgtt
    41881 caccgtcgat aatccagtcg gcgttaaaaa ttttgtggcg gcagtgctca gagttcgcct
    41941 gagcgaacat atacagctcg atgtcgttcg ggttacgacc aagctttgtg aaagcatcct
    42001 gcagatagtc aatttcatct tccgccagag ccaagccaag acgcaggtta gcgtcgatca
    42061 gcgcctgacg gccctgcccc agcaaatcaa cgcttgtgac cggagtcggt tgatggtggg
    42121 caaacaactg ctctgcatca tctaaatcaa aaaagaccgt ttccatcatg cggtcgtgca
    42181 gttcagcggt aacctgctgc cattgttcat tggtcagcgt accggcttct atatagtaag
    42241 caacgccgcg ctcaaggcgg tttacctgtt gtagcccgca gttatgggca atatcggtcg
    42301 ctttcgaaga ccagggagag atggtgccag gacgcggggt caccagcagg agtttgcctt
    42361 gcggggcgtg gctggcgagt gccggaccat atttcagcag gcgttcaagt tgtgcgtgct
    42421 catcatcgtt taacggcgca ttgaggtcag caaaatggac atactcggcg taaatattgt
    42481 gaaccgggag cctggcagcc tgaaaacgtg ccagcagttt gttgattcgg aatgccgaca
    42541 gtgcaggcga accacgcaga atttccatca taagtctctc gtcttctaag ctttcggtgt
    42601 acccaagggg ggggaaacgg gcgtcattat aaagaatctg atgcgctgac gaaaccgttt
    42661 gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg ccgccaatgg ctgtaataag
    42721 ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt atgagtaaac ctttcactat
    42781 tattacgttt tttcaagctg ggacgcgcac gacacagaga attaactaat tgaaaaaatt
    42841 aaagattaat tatctgttca tcggcattct ggcactgctg ctcgcggtcg ctctctggcc
    42901 atccattccc tggtttggta aagccgacaa ccgtatcgcc gccattcaag cgcggggaga
    42961 gttgcgtgtg agcaccattc atactcccct gacttataac gaaatcaacg ggaaaccttt
    43021 tggcctggat tacgaactgg cgaaacagtt tgccgattac ctcggcgtaa aactgaaagt
    43081 gaccgtgcgg cagaatatca gccagctgtt tgacgacctt gataatggta acgccgacct
    43141 gctggcggca ggactggtct ataacagtga gcgggtaaaa aattatcagc ctggccctac
    43201 ctattattcc gtgtcacaac aactggttta taaagtgggt cagtatcgcc cacgtacgct
    43261 gggcaacctg acggcggagc aactcaccgt tgcaccgggt catgtggtag ttaacgatct
    43321 ccagaccctg aaagaaacaa aattcccgga attaagctgg aaggtagacg acaaaaaagg
    43381 ctctgcggaa ttaatggaag atgtcatcga aggaaaactc gattacacca ttgctgattc
    43441 tgtcgccatc agcctgtttc agcgcgttca cccggagctc gccgtagcgc tcgatatcac
    43501 cgatgaacaa ccggtgactt ggtttagccc gttagatggc gataataccc tttccgccgc
    43561 cctgctcgac ttcttcaacg aaatgaatga agacggtacg ctggcacgca ttgaagagaa
    43621 atacctgggg catggcgatg attttgatta cgtcgatacg cgcacatttt tacgcgccgt
    43681 cgatgcggta ctgccgcagt taaagcccct gtttgagaaa tacgccgaag aaattgactg
    43741 gcgtttgctg gccgctattg cttatcagga atcgcactgg gatgcacagg ccacttcacc
    43801 gacgggtgtg cgcggcatga tgatgttaac caaaaatacc gcgcaaagcc tcggcattac
    43861 ggatcgtacc gatgccgaac agagcatcag cggtggcgtg cgttatttgc aggatatgat
    43921 gagtaaagtg ccggaaagtg tgccggagaa cgaacggatc tggtttgccc tcgctgcgta
    43981 caatatgggc tatgcgcata tgctggatgc ccgcgccctg acggcaaaaa ccaaagggaa
    44041 tcctgacagt tgggctgacg taaaacagcg tctgccttta cttagccaga aaccctatta
    44101 cagcaagctg acttacggct acgctcgtgg gcatgaagcc tacgcttatg tcgaaaatat
    44161 tcgtaagtat cagattagcc tggtgggtta tctgcaagag aaagagaagc aggctacaga
    44221 agcggcgatg caactggcgc aggattatcc ggcggtatcg cctacggagt tgggcaaaga
    44281 gaaatttcct tttctctcgt ttctttccca gtcgtcatca aactatttga cccattctcc
    44341 ctctctgctg ttttccagga aagggagtga agagaaacaa aattaatccg tcgaggattg
    44401 cgcttttttc tgcgctttaa tttcctggcg gcgcatgcga aagaagtcac tgagcaacgc
    44461 cgcgcactca tccgccagta ttccttccgt aatttccact cggtgattca tacccggatg
    44521 atgcagcaca tccattaaag atcccgcagc gccagttttc gcgtcacgcg caccaaagac
    44581 cacgcgacca atgcgactgt ggatcatcgc tccggcacac attacacatg gttcaagcgt
    44641 gacatacaac gtggcgtcga tcagacgata attttgcatc accagaccac cctgccgcag
    44701 ggccatgatt tctgcatgtg cggtgggatc atggcgacca atcgggcggt tccagccttc
    44761 gccgattacc cgattgttat gcactaatac cgcgccgacc ggcacttccc gctcatccca
    44821 ggcacgtttc gccagcgtca gcgcgtgacg catccaatat tcgtggctaa attcgacttc
    44881 agacaaaaag aaaactccgg ttataaaagc gcggcgcatt atacacggac gctatgcttt
    44941 actctattcc agttgctgga gttcaccgcg cggggttact cgccaacgat gctgacagaa
    45001 ataaagcagc ggattgtcct gattactgtc gctatagcca ctgtacagcc gcagcggcgt
    45061 gccgattttg cgctccagtt gtgcgacctt ttcatgtccc agacaacgca tcgtcaatac
    45121 ccaaccacca tagccacgct gaatttggct ggcgataaga ttaacccgcg gcagccaggg
    45181 cgtatcgaaa taaaccgctt caaccagcgg ctgcggagag ccggtaatca accagatatc
    45241 agcatcggaa cttaacaggt aggtggttaa tcgctcctga accagcggaa aggcggtaac
    45301 attgtcgcga aaccagcgca cgaaatcggc ctgcaacgtc tgtaaacgtg cttcgctgtg
    45361 accaaaagtg cacccccaca gaagcagact catcggccag cgtgccgcac gaccttttat
    45421 caataacgca atggctataa tcggtaacaa cggcaggaca agtaacgcat tcagcggttg
    45481 gcgacgcagt aaatagcgca gaaaactgcc gaacatatcc tgctgatgca atgttccatc
    45541 taagtcaaaa aacaccacac gacgctcgtg agttgccaaa ccatactcct ctggactgaa
    45601 acatcctgat taattttcgc tactacatag cctaacagat agatcatcac ttttccggca
    45661 acaatcgacc gcatcatggc taactggaat ttttaattca tggcaattag cggcaatgga
    45721 atataaaatt cactcgcgtg tgtctcatat tgcctgttgt cgccactatt tcatcgcgag
    45781 gaaagatatg aacggcttac ttcgtatccg tcagcgttac caagggcttg cccagagtga
    45841 taaaaaactg gcggattatc tgctgctaca acctgatacg gcgcgccatt taagctctca
    45901 gcaactggcc aacgaagccg gagtcagtca gtccagcgtc gtgaagttcg cgcaaaaact
    45961 cggctataaa ggttttccgg cgcttaagtt ggcgttgagt gaagcgctgg caagccagcc
    46021 ggaatcgccc tccgtgccca ttcataacca aatccgcggt gatgatccgt tacggctggt
    46081 cggcgaaaaa ctgattaaag aaaataccgc cgctatgtac gcaacgctaa acgttaatag
    46141 tgaagagaaa ctgcatgaat gcgtaacaat gttgcgctct gcgcggcgga taattctgac
    46201 cggtattggc gcttcgggtc tggtggcgca aaactttgcc tggaagctga tgaagattgg
    46261 cttcaatgct gccgcactgc gcgatatgca tgcgctactc gcaacagtac aggcgtcgtc
    46321 ccctgacgat ctgttattag ccatttccta caccggtgta cgacgcgagt taaacctggc
    46381 ggcagatgag atgctgcgag tgggcggaaa agtgctggcg attaccggct ttactccgaa
    46441 tgccctgcaa cagcgtgctt ctcattgcct gtataccatt gccgaagaac aagcgacaaa
    46501 cagtgcttca atctctgctt gtcacgctca gggaatgtta acggatttgc tgttcattgc
    46561 gctgattcag caggatctgg aactggcacc agaacgtatt cgtcatagtg aagcgctggt
    46621 gaaaaaactg gtctgagtaa agaatgcgcg tataatgccc gcccggtttg tgttgttttg
    46681 agagtttcct tatggcgctg ttaatcacta aaaaatgcat caattgtgat atgtgtgaaa
    46741 tcaaataaaa acacatttat tttcatagtg atacagcaat gtgttattga atgtgttgtt
    46801 ggatttatgg tgcaaaaaaa ttttgcataa gttcagcaac acaaagaaca ccactttctt
    46861 taacgtcctc cgcgccctaa aggacggggc tttacggcgc aaaaggcctg tgacatacaa
    46921 cattaacttt tcagtcaacc gtagcacgtt cttgtatacg ccgtgtctgc ggttttatat
    46981 aaccctggct ggaaacctct tatacaaagt cgattcgcca acatcataga tgatcgccac
    47041 ctggcgagaa actcctgatg caattagtcg cccggcctgc gcccattatt ctggtgaaag
    47101 tttgggacgg cgaccgccaa ttcgtccctg tgcgcgagca gcttccagtc ctgcttttgt
    47161 tcgttcaaca atcagttcac gttccatttc agccagggca cccatcacat ggaagaaaaa
    47221 gcgtcccatt ggtgtgctgg tatcaattga atcagtcaga ctacgaaagt tgatgcctcg
    47281 ttcgcgcaac gcctccacca gcacgacaag atgccgcata ctgcgcccca gccgatccag
    47341 cttccagaca accagtgtgt cgcctgccga taatgtcttg agcagttttt tcagccccgg
    47401 tctttcggac tttgtaccac ttatcttgtc ttcaaaaatc agctcgcatc ctgcacagtt
    47461 caacgcatta cgttgtagat cggtattctg gtcatttgtt gatacgcgta ca
//
LOCUS       DAJTSF010000021        45785 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000021 DAJTSF010000000
VERSION     DAJTSF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.021"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            95..2248
                     /locus_tag="OO335_001262"
     CDS             95..2248
                     /locus_tag="OO335_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6001721.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..4031
                     /locus_tag="OO335_001263"
     CDS             2301..4031
                     /locus_tag="OO335_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001722.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="OO335_001264"
     CDS             complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="OO335_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6001723.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4750..5661
                     /gene="panE"
                     /locus_tag="OO335_001265"
     CDS             4750..5661
                     /gene="panE"
                     /locus_tag="OO335_001265"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6001724.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5624..6214
                     /gene="yajL"
                     /locus_tag="OO335_001266"
     CDS             5624..6214
                     /gene="yajL"
                     /locus_tag="OO335_001266"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6001725.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="OO335_001267"
     CDS             complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="OO335_001267"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6001726.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKVEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7922..8164
                     /gene="xseB"
                     /locus_tag="OO335_001268"
     CDS             7922..8164
                     /gene="xseB"
                     /locus_tag="OO335_001268"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6001727.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8164..9063
                     /gene="ispA"
                     /locus_tag="OO335_001269"
     CDS             8164..9063
                     /gene="ispA"
                     /locus_tag="OO335_001269"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6001728.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9088..10950
                     /gene="dxs"
                     /locus_tag="OO335_001270"
     CDS             9088..10950
                     /gene="dxs"
                     /locus_tag="OO335_001270"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6001729.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11005..11922
                     /gene="yajO"
                     /locus_tag="OO335_001271"
     CDS             11005..11922
                     /gene="yajO"
                     /locus_tag="OO335_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6001730.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="OO335_001272"
     CDS             complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="OO335_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6001731.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="OO335_001273"
     CDS             complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="OO335_001273"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6001732.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="OO335_001274"
     CDS             complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="OO335_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6001733.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="OO335_001275"
     CDS             complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="OO335_001275"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6001734.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="OO335_001276"
     CDS             complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="OO335_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6001735.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="OO335_001277"
     CDS             complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="OO335_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6001736.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16289..16828
                     /locus_tag="OO335_001278"
     CDS             16289..16828
                     /locus_tag="OO335_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6001737.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17126..18010
                     /gene="tsx"
                     /locus_tag="OO335_001279"
     CDS             17126..18010
                     /gene="tsx"
                     /locus_tag="OO335_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6001738.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18187..18534)
                     /locus_tag="OO335_001280"
     CDS             complement(18187..18534)
                     /locus_tag="OO335_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6001739.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18663..19634)
                     /gene="secF"
                     /locus_tag="OO335_001281"
     CDS             complement(18663..19634)
                     /gene="secF"
                     /locus_tag="OO335_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6001740.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19645..21492)
                     /gene="secD"
                     /locus_tag="OO335_001282"
     CDS             complement(19645..21492)
                     /gene="secD"
                     /locus_tag="OO335_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6001741.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="OO335_001283"
     CDS             complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="OO335_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6001742.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="OO335_001284"
     CDS             complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="OO335_001284"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6001743.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23057..24127)
                     /gene="queA"
                     /locus_tag="OO335_001285"
     CDS             complement(23057..24127)
                     /gene="queA"
                     /locus_tag="OO335_001285"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6001744.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24220..24793
                     /gene="acpH"
                     /locus_tag="OO335_001286"
                     /pseudo
     CDS             24220..24793
                     /gene="acpH"
                     /locus_tag="OO335_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="OO335_001287"
     CDS             complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="OO335_001287"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR6001745.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26771..28144)
                     /gene="proY"
                     /locus_tag="OO335_001288"
     CDS             complement(26771..28144)
                     /gene="proY"
                     /locus_tag="OO335_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6001746.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="OO335_001289"
     CDS             complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="OO335_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6001747.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="OO335_001290"
     CDS             complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="OO335_001290"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6001748.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="OO335_001291"
     CDS             complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="OO335_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6001749.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32178..33380
                     /gene="sbcD"
                     /locus_tag="OO335_001292"
     CDS             32178..33380
                     /gene="sbcD"
                     /locus_tag="OO335_001292"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6001750.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33377..36520
                     /gene="sbcC"
                     /locus_tag="OO335_001293"
     CDS             33377..36520
                     /gene="sbcC"
                     /locus_tag="OO335_001293"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6001751.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36646..37827
                     /gene="araJ"
                     /locus_tag="OO335_001294"
     CDS             36646..37827
                     /gene="araJ"
                     /locus_tag="OO335_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6001752.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37970..38878)
                     /gene="mak"
                     /locus_tag="OO335_001295"
     CDS             complement(37970..38878)
                     /gene="mak"
                     /locus_tag="OO335_001295"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6001753.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39003..39914
                     /gene="rdgC"
                     /locus_tag="OO335_001296"
     CDS             39003..39914
                     /gene="rdgC"
                     /locus_tag="OO335_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6001754.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39992..40447)
                     /locus_tag="OO335_001297"
     CDS             complement(39992..40447)
                     /locus_tag="OO335_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001755.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40444..40779)
                     /locus_tag="OO335_001298"
                     /pseudo
     CDS             complement(40444..40779)
                     /locus_tag="OO335_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="OO335_001299"
     CDS             complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="OO335_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR6001756.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41290..41967)
                     /locus_tag="OO335_001300"
     CDS             complement(41290..41967)
                     /locus_tag="OO335_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6001757.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="OO335_001301"
     CDS             complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="OO335_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6001758.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="OO335_001302"
     CDS             complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="OO335_001302"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6001759.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43173..43631)
                     /locus_tag="OO335_001303"
     CDS             complement(43173..43631)
                     /locus_tag="OO335_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6001760.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43751..44560
                     /gene="proC"
                     /locus_tag="OO335_001304"
     CDS             43751..44560
                     /gene="proC"
                     /locus_tag="OO335_001304"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6001761.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="OO335_001305"
                     /gene_synonym="dgcC"
     CDS             complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="OO335_001305"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR6001762.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11899 a        11945 c        11203 g        10738 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     3181 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     3241 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     3301 tggcgtttct gatgacagca ccagaatacg cttgttataa tagtcagtat tgttatgaag
     3361 acaaagtcag cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat
     3421 atttcacgac tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa
     3481 taagtaatac tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg
     3541 catctttcgc tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga
     3601 gaggactatt ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa
     3661 accgagtgtt ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta
     3721 taaatgccaa agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta
     3781 tgcataatct gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac
     3841 acattacctt tacgcttaat aaattaacaa cacaaccagc aataacaaaa atagatagcg
     3901 tgggtaatga gcaactttat cagattggca aaatctacca taagttggaa attaaacctg
     3961 agccagagca acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg
     4021 caactcaata aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat
     4081 atccagaaag ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc
     4141 gcaccatcgc catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat
     4201 cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt
     4261 tctgagtggc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc
     4321 tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct
     4381 tggcacgcag aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga
     4441 tggttttgct ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg
     4501 actccacttc gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag
     4561 agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata
     4621 cctgcaaagt taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt
     4681 cgcaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga
     4741 ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca
     4801 gcactttgta aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt
     4861 agtgtgaatc tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat
     4921 cccgattttc tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc
     4981 gatgccgtca aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac
     5041 aacggcatgg gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc
     5101 accacccatg ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg
     5161 catatcggtc cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa
     5221 accgtgttgc ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg
     5281 gccgtcaact gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta
     5341 cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc
     5401 gaagggcatc atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc
     5461 acagcagaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc
     5521 gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa
     5581 aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact
     5641 ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt
     5701 tcgcggcggt atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac
     5761 ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga
     5821 atatgacgtg atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac
     5881 tctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg
     5941 cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt ccgattggca atatgaccgg
     6001 cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg
     6061 ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct
     6121 gaaaattatc gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt
     6181 gatggcggca gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct
     6241 gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc
     6301 ttgctcgcgc agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag
     6361 ccaggttttg ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga
     6421 aaccacatca atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat
     6481 atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg
     6541 ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc
     6601 gagaatactg aagtcgaact tctcttcttc cgcttcaacc ttcgatttaa ccgctttcac
     6661 cgtcgggctt ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt
     6721 gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag
     6781 gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac
     6841 ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga
     6901 tgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc
     6961 gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct
     7021 gccaaagcgg ttccacagat aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc
     7081 gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga
     7141 aacaccggag tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg
     7201 gaaaccacca ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc
     7261 cagatgaaca gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt
     7321 taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg
     7381 cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt
     7441 ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat
     7501 acccggaata cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg
     7561 aacttcgatg ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac
     7621 gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat
     7681 ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc
     7741 cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc
     7801 ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg
     7861 ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat
     7921 tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca
     7981 gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga
     8041 acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt
     8101 acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga
     8161 gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc
     8221 cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc
     8281 gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt
     8341 ggcgttagca caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac
     8401 tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg
     8461 acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg
     8521 ctggcgttct cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt
     8581 tcgatgattt ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca
     8641 ttagatttag acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt
     8701 cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat
     8761 aaagggcgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc
     8821 caggttcagg atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag
     8881 ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc
     8941 cggaagaaag cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa
     9001 cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa
     9061 taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg
     9121 gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc
     9181 tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc
     9241 tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt
     9301 gaccaattga tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc
     9361 cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc
     9421 gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt
     9481 ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg tgtgattggc
     9541 gacggcgcga ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgt
     9601 cctgatatgc tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg
     9661 cttaacaacc atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc
     9721 gggaaaaaag ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa
     9781 catattaaag gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc
     9841 ggcccggtgg acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac
     9901 ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca
     9961 gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg
    10021 ccgaaaagta gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa
    10081 acggcagcga aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc
    10141 atggtcgagt tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa
    10201 cacgcagtga cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc
    10261 tactccacct tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag
    10321 ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac
    10381 cagggcgctt ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg
    10441 agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg
    10501 tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa
    10561 ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt
    10621 ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat
    10681 atgcgttttg tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa
    10741 gcgctggtca ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa
    10801 gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt
    10861 attccgcaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg
    10921 gaagccaaaa tcaaggcctg gctggcataa tccctactcc actcctgcta tgcttaagaa
    10981 attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc
    11041 gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca
    11101 tggacactgc cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata
    11161 aatttctttg ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc
    11221 gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc
    11281 gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc
    11341 ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac
    11401 acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt
    11461 tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa
    11521 cagcactgct gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa
    11581 gaagagcgcg agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc
    11641 ccgctggcgc gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct
    11701 gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag
    11761 cggttaacgg gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg
    11821 ttgttaagta aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt
    11881 gatgagctgc tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa
    11941 acgccgtata aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg
    12001 tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga
    12061 tgaccgataa agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg
    12121 atccccatgc cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata
    12181 tccagaatac ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc
    12241 gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc
    12301 acgcccatat ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc
    12361 accaccagcg agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc
    12421 gccagcgatc ccatcgagcc aggaacgatt gggcttaatc cacttccgaa tccgacagca
    12481 agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac
    12541 aaaatggtca tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag
    12601 cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag
    12661 agcaacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc
    12721 accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc
    12781 agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg
    12841 cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag
    12901 tgcctgcccc tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc
    12961 atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc
    13021 gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc
    13081 tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt
    13141 atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa
    13201 ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg
    13261 atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc
    13321 agcaggatcg atatcaggga ggaaatggtt gcccgccacc agcgtatcag tgctgatcgc
    13381 cagggtctgt ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac
    13441 atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca
    13501 tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt
    13561 ccgtgaatct accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta
    13621 tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg
    13681 atcgcttcgt taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc
    13741 gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag
    13801 ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg
    13861 aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca
    13921 gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg
    13981 cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt
    14041 caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt
    14101 ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac
    14161 cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata
    14221 ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt
    14281 accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac
    14341 aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc
    14401 cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc
    14461 cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc
    14521 ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag
    14581 atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt
    14641 taaattgggg ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt
    14701 cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta
    14761 gcaatacgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac
    14821 ccagttgcat catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg
    14881 tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc
    14941 cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac
    15001 gtactggctg gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag
    15061 accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg
    15121 catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact
    15181 ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa
    15241 tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac
    15301 tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc
    15361 cagcgacctg cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga
    15421 tgagtgcgtc acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat
    15481 acgccgttgc gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg
    15541 gttcacccgc tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc
    15601 acccgacatt cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc
    15661 gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa
    15721 attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat
    15781 cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct
    15841 caccggtggc gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac
    15901 tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg
    15961 gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg
    16021 tggtgaaacg ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc
    16081 ccacgagacg agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac
    16141 ggcgtcctga ccaggtggtt aacggaaatc tattttaccc tgaactgacg atacaacaaa
    16201 ggcgcaacgc ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag
    16261 gctaattgcg acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct
    16321 gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca
    16381 cagcgtcagc acgctgaacc aggagatgac gcaactcaat caagaaacag tcaaaatcac
    16441 tcagcaaaac aggctgaatg caaaatccag cagtggggtt taccttctgc ctggagcgaa
    16501 aacaccggca agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac
    16561 gcctgataca gatggcacca cccttacact acgtattcag ggtgagtcga atgatccctt
    16621 gcccgcattc agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaactttca
    16681 ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga
    16741 tgttgatatt ccgttacaat taaaggggat ttctgtggat cagttaggct ttgtacgtat
    16801 ccacgacatt caacctgtta tgcagtaaac gtatttcggg acgattttgt gcgtcccgca
    16861 acatcttccc cgtcattttg ttactctgct tacatcacct ggattgatag taaaagtttg
    16921 caacaagggc gagagtcagt acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg
    16981 attacgtaaa tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa
    17041 taggctcctc tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa
    17101 gggattttca aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact
    17161 ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc
    17221 cgactggtgg caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca
    17281 gatccgcaac gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt
    17341 ctatggttat gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg
    17401 gaaccacggt tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac
    17461 caatactgac cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta
    17521 cgacatgggt cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga
    17581 tatcgacact ggcctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca
    17641 gaactatggc gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt
    17701 tgtgccgatt accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga
    17761 ctggggttcc gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac
    17821 taataactct atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc
    17881 tgtcgtagct cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt
    17941 cggcaacggc aacttcaacg ttcgctctac cggctggggt ggttacctgg tagtaggtta
    18001 caacttctga ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt
    18061 gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa
    18121 ttcaatatat tgctgaaata acgtaggcct gataagcgta gcgcatcagg caattttacg
    18181 ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc
    18241 ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc
    18301 ttcggtatat ttcgaatgct catgatcgtg gcaatagaga cacaacaatt cccagttact
    18361 gccatcttcc gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag
    18421 gttagaataa acaaactcgc gggaacagcg accgcagacc cacggataga tttttaatgc
    18481 tttttcgcga tagccgcttt ctaaccgtgc atagtttttt gggatgatag ccatgaaaag
    18541 ttacctggat gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc
    18601 tccatacatc aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt
    18661 gattaaggca gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg
    18721 tgttcgcgct tcatacccag tttcagagcc aacgcagatg ctacgtagat ggaagatgca
    18781 gtgccgatgg aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg
    18841 aagagataca gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc
    18901 agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg
    18961 aagttttcac gaattcggtc cgataccacg atactgtcgt taagcgagta accgataacc
    19021 gacatcaacg atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc
    19081 agcgtaatga taacgtcgtg cgtcagcgca ataaccaccc ctgccgccag tcgccactca
    19141 aagcggaaac ctacgtacac gaggatagac agcagcgctg ccatcaacgc catcgcaccg
    19201 gtttgcgcaa ggtcagcccc cacgctcgga ccgacgaact caatacgctt cactgctgca
    19261 ttctgattgg tggattcgtt aatcaccttc agaacttggc tgcccagcac ctgaccgccg
    19321 gtttcgcctt cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc
    19381 agcattggct cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc
    19441 ggtttttcga gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag
    19501 ccgcgcacgc ccataataac gatagcagcg attaacagca gaccagagat gccgaaagcc
    19561 cagtagtccc agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta
    19621 tattcctgtg ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata
    19681 tagcaggttt acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc
    19741 gataccggta gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat
    19801 gactttaatc agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc
    19861 ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt
    19921 gatcagtacg ttcgcatcga ccgctaccgc aagggttaag acgatacccg cgatacctgg
    19981 catactcagt gttgcgcctg gcaacagcga cataataccg acgatcaaga tcaagttggc
    20041 aatcagagca ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat
    20101 agaaaccagc agaccggcaa ggcaagcctc cagcccctgc tcaatgttct gcatacccag
    20161 ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg
    20221 cagcagcagt gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct
    20281 gttacccaga cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac
    20341 cgcacgacca tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat
    20401 cggtttgccg atgttgtcct tggtgaagtt agacataatg ttgccaccag cgctatcgag
    20461 cgagatgtta acctgcggct ggttgtattc gtcctggctg gaagtggagt cggtgatatg
    20521 gtcaccagtc agaattacgc gtttgtacag cacaaccggc tgaccttcgc gggtctgttt
    20581 cacttcagag tcgcccggta cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt
    20641 taccagacgg aattccagcg ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc
    20701 ctgaataccg ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc
    20761 cgccacgcca agttggttta cacggttacg caggatatta atgttctgct gcaccgcata
    20821 ttcacgcgct tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg
    20881 gctgctaatc accaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc
    20941 tttagcatcg cggaaagtga tgctcaggcc gtagttgttt tctttacgaa cagtggtata
    21001 cgggatgccc ttttcgcgca ggtcactgcg taggctatcg atattttgtt cctggagttt
    21061 gccaagcgcg gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag
    21121 gccgagcttc atcggctcag catgaatagc tgccagccag cgcggcgttg ccggggcaag
    21181 gttaagcgcc acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa
    21241 ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt
    21301 agcagttatt ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc
    21361 ggcgactccg cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag
    21421 cgcatacagc agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata
    21481 acggtttaac acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc
    21541 tttcggcagg acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag
    21601 cgcgatagca atgtagccgt tttccgctac tttggttacg cgaccaacca ggccaccgtt
    21661 cgtcagaact tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg
    21721 cttctgctgt ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa
    21781 aatcaaagac atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat
    21841 aaaaaagctc attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt
    21901 ctcgcccctg acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatag
    21961 ccttgcgtaa acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc
    22021 gcgcgcctaa tatttcgttg caacggtcga gatgatgcaa gtaagcgcgt gaataattgc
    22081 gacaggtgta gcaatcacac tcaggatcga gtgggccagt atcgctctta tatttcgcat
    22141 tgcggatttt caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg
    22201 gcattacgca gtcaaacata tcgataccgc gacgtacgcc ttcaaccagg tcttccggtt
    22261 taccaacgcc catcaggtaa cgcggtttgt ctgccggaat ttgcggacac acatgctcca
    22321 gaatacggtg catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat
    22381 caaagccgat atctaccaga cctttaacag atatatcacg taaatcttcg taaatactgc
    22441 cctgaattat accaaataac gcatttttgt taccaagact gtcaaaacgc tcacggctac
    22501 gcttcgccca acgcagagac atctccatag agcgttttgc gtaatcccag tcagcaggat
    22561 acggcgtaca ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca
    22621 tcgacttttc cgggtcgagg aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc
    22681 cctgttcggt gattttacga atatcaccaa ggctgaagac ctggaagccg ccggagtcgg
    22741 taagaatcgg tcctttccac tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt
    22801 cctggcccgg gcgcagccac aggtggaagg tgttgccgag gataatttgc gcgccagtgg
    22861 cttcaacttc ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac
    22921 aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg
    22981 tggtgtccag ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgttta
    23041 aaccagcgcc gcggaattac tccccgacgc gctcattaat tgcctgcgga ttgtacgtga
    23101 taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc ttctaccgcc gctttatagg
    23161 cattcatggt gtgttgataa ccggcaaagg ccgaaaccag cataatcagc gtcgactctg
    23221 gcaagtggaa gttcgtcacc agcgcatcga ccactttgta ctggaagccc ggatagataa
    23281 agatttgggt atcgtcaaag aacggttcga taagatcgtt ttttgccgcc tgtgccgcgc
    23341 tttccagcga acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg
    23401 ccgccagtac cgcgtctacc acatcctgcg gtacttcagc gtattccgag tgcatgatgt
    23461 gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca
    23521 caaacgccat ctccacgcct ttggcgcgta atttttccag caaaggctcg tcaaaatgca
    23581 gaccagcggt cggggctgca accgcgcccg gtttttcgct ataaacggtt tgataaagtt
    23641 cgcggtcagc gtcttcgtcc ggacgatcga tatacggcgg cagcggcata tggccgatgc
    23701 tgttgaggat atccagtacc gagcgttcat cattaaattc gacttcaaac agtgcgccgt
    23761 ggcgcgcggt cattgttgcg ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag
    23821 gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct
    23881 caaccagcac ttcaatcttg ccgccgctgg ctttacgccc aaacagacgc gccgggatca
    23941 cgcgggtatt attaaaaacc agaagatcgc cggggttgag cttatcgagt aaatcggtga
    24001 aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga cagctactgc
    24061 gttcaggcat gggatagtgg gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa
    24121 cgcgcatgac actgactcag actaaaataa gagtcgggta gtctagtgcc ggggcgctct
    24181 ccctgcaacc tttacccccc tttggataga atataatcga tgaatttttt agctcacctg
    24241 catttagccc atctcgcgga aagctcgctt tctggcaatt tactggctga tttcgtacgc
    24301 ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg gcattcatat gcatcgacgt
    24361 attgactgac aatctgccgg aagtccgcga agcacaggag tggtttcgta gtgaaacgcg
    24421 ccgcgttgcg cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc
    24481 gcagctgtcg ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat
    24541 gacgattttg ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga
    24601 gcagtggctg gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc
    24661 aagccgccgc ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta
    24721 tgacgccctc gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca
    24781 caaggcgtta taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga
    24841 atcccgtcag tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagaaac
    24901 ggtgacgcgg gtagcaccac ttcactggcc tcgccacggt tgattgccac cagtacacgt
    24961 tgctgattca gcacgcggac aaataccact acgttatctt ccgcatacag cacctgacag
    25021 ccgccacgac gcagcgcctg acttttctta cgcagcacaa tcattcgccg gtacagcgcg
    25081 aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg
    25141 tttttaccat ccagtcccac ttcatcaccg taataaatgc acggtacacc aggccaggtg
    25201 aacagccaga ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat
    25261 cgcgcagtat cgtggctatc gagctgatta aacatacgta attgttgttg atgagaaagc
    25321 cctgcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg ctgcggatca
    25381 taagagatgt cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc
    25441 atggcagcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg
    25501 acaatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccggc aacgtgctgc
    25561 atattattgc gcgccccacc cgcctccccc agcatatgca ccacatccag ccgccagccg
    25621 tccatattcc acggcgcttt cagccagtgg cggacaatac tgtcttcccc gcgataaatt
    25681 tcattcacca gactttccga ctgataatcc agcttcgtca agctggcata gccaagccag
    25741 tcgagcgccg tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagagttg
    25801 tgacaagctc cccccgtgcc aagattgtgc ctgtcaaacc aggcatggga atcgccactg
    25861 tggttaaaca cgccgtccag caccagccgc attcccagct gctgcgtatt gtgtcgcaaa
    25921 cgcagcaacg ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg
    25981 tatttatgta cgctgggagc tttaaacacc ggattgagat acagcgctgt cacgccaagc
    26041 tttttcagat acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat
    26101 ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct
    26161 gcatgatggt aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga
    26221 tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat atcaggtaca
    26281 tcgacggcaa actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac
    26341 caacgctggc gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg
    26401 gagagatcaa tcgccgcacg ccatgcggtg acgcctggct gcggctgact gcgctgctta
    26461 tgcattggta ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt
    26521 gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat
    26581 gggggcaccg gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat
    26641 caccttgcca tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg
    26701 acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag
    26761 aacgcaagta ttattggttt tcagccagct gacgatcgtg gcggcgttta aacatccagc
    26821 caatcaacag cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt
    26881 gataaccaat caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta
    26941 ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga
    27001 aggcgatttg cgacagcagg atcataatcc acacccatac cgtggcgaag gttgccagcg
    27061 aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca
    27121 gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga
    27181 aaattttcgg tgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata
    27241 cgtcactgtt aattgccgac agcgaagcgg tcagcacaac aaagttaaga atgctggcgg
    27301 caaaggtaat gcccatatgc tggaacgtca gtacgaacgg gctaccggca gtgccaacct
    27361 gattccacgg gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac
    27421 gcatcggcac ggagttaatc gcacgcggaa tcgatttctc aggatctttc gcttcaccgg
    27481 cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatta
    27541 ccatgccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg
    27601 tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga
    27661 tgatgatggt ggcgacttta aagaacgaga accagaattc cagctcaccg aagaccttca
    27721 cgctcatcag gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg
    27781 gcaccgtcgg gaacaagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga
    27841 caataaggat ttcaaaacag taggtccatc cggtaatgta acctgccagc gggccaaggt
    27901 tttcctgcgc ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca
    27961 gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg
    28021 gaccggccat tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac
    28081 ccagtgccat aaagcgtatg tggcgggtac ttagcccacg ctttagctta ttcttacttt
    28141 ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt
    28201 aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat
    28261 cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt
    28321 cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga
    28381 tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg
    28441 aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa
    28501 tagcggtcag caccggtacg gagatctgga tcagctggct taagccgagg ttagacacca
    28561 ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg
    28621 caaagaattc tgcacaagca caggtcaggc caaccgccgt gaccagacag gcgatgaaga
    28681 ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca
    28741 gaatagcagc gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacaga
    28801 acagcgccag gtacagcaaa gtcagaccaa cacccgccat caggccagcc cagacggtat
    28861 aacgggtcag cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga
    28921 taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag
    28981 aaaatgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa
    29041 tcgcgacaac agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca
    29101 cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca
    29161 ggctgtagat aaacagcggc agcgcggaat cacccgtcag cggcgcaatc cccacttcaa
    29221 aggaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg
    29281 ttgccagcag tacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc
    29341 caacttttgc cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc
    29401 cgaatgccgc agtccagacg tgttcgcctg cctgtaagcc gaccattgga gggaaaataa
    29461 tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg
    29521 atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt
    29581 tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac
    29641 tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg
    29701 acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa
    29761 accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa
    29821 agacatggct aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat
    29881 tttgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa gatgacaaag
    29941 gcggattaat cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt
    30001 ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc
    30061 acgatcgcta accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga
    30121 taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca
    30181 acgctaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttcc
    30241 ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg
    30301 ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc
    30361 agagtctgag cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg
    30421 agcaattgcg tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct
    30481 tccatccgct gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc
    30541 agcggctgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac
    30601 tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg
    30661 acatcacgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga
    30721 tgccgcccgg tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat
    30781 tgcgtaaact ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag
    30841 cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga aaataccgcc ctcttccgtg
    30901 gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg
    30961 cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc
    31021 ggttcccagc taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat
    31081 gaaaggcgca ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa
    31141 aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc
    31201 agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag
    31261 aaatcataag ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga
    31321 tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta
    31381 cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag
    31441 acgtggttta acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc
    31501 agcagtttaa attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga
    31561 gatgtcgggt tgagacttaa tccctgcatc ttaatcacct cttccaccgc cattggcgaa
    31621 atacggcgca ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa tggcttggtg
    31681 atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg
    31741 gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttgatgaac
    31801 tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca
    31861 ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt
    31921 tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt
    31981 ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat
    32041 gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt
    32101 tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg tataatcgcg accatgcttt
    32161 ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact
    32221 tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag
    32281 cacaaaccca tcaggtggat gcgattattg ttgctggtga tgttttcgat accggctcgc
    32341 cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct
    32401 gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg
    32461 atatcatggc gttcctcaat acaaccgtgg tcgccagcgc cggacatgcg ccgcaaatct
    32521 tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgcccgc
    32581 gtgacattat ttccagccag gcggggctta acggtattga aaaacagcag catttactgg
    32641 cagcgattac cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc
    32701 agcctctgcc catcatcgcc acgggacatt taacgaccgt gggttccagt aaaagtgacg
    32761 ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag
    32821 ccgactacat cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg
    32881 ttcgctattg cggttccccc attccactga gttttgatga atgcggtaag agtaaatatg
    32941 tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg
    33001 taactcaacc catggcggtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac
    33061 agtggcgcga tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg
    33121 agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag
    33181 tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg
    33241 aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagacc
    33301 tggatgaatc gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cgtaccctcg
    33361 ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa
    33421 aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgccat
    33481 taccggccca accggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta
    33541 tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga
    33601 taccgctgaa tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt
    33661 ctggagccag aatcgggcgc gtaaccagcc ggacggtaat ttgcaggtgc ctcgcgtaga
    33721 gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact
    33781 gacagcgacg ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc tgctttcgca
    33841 ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga
    33901 gttaaccggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc
    33961 ggcccgcact gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc
    34021 ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt
    34081 aattaccgcg cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt
    34141 gcagcaagaa gccagccgcc gtcaacaggc cttgcaacag gcgttagccg aagaagaaaa
    34201 agcgcaacct caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca
    34261 ctgggaacgc atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga
    34321 agtaaatact cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc
    34381 gaagcagtca gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca
    34441 cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca
    34501 aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa
    34561 acttaatgcg cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctaccgccct
    34621 ggcgcaacat gccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat
    34681 tgttccccaa caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga
    34741 acagacgcaa cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca
    34801 gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc
    34861 ccagcgtgca cagttacagg cgggtcagcc ttgtccactt tgtggttcca ccagccaccc
    34921 ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct
    34981 ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc
    35041 attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca
    35101 agcacttact caacaatggc aagccgtcac ggccagcctc aatatcacct tgcagccaca
    35161 ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact
    35221 cagccaacga catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta
    35281 tcaacagcaa attgaacaac gtcagcagca gctcttaacg gcattggcgg gttatgcact
    35341 gacattgcca caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca
    35401 gagctggcag caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac
    35461 accgattctg gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc
    35521 gctggataac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac
    35581 gttacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccaag cgcagtttga
    35641 caccgcgcta caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga
    35701 tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca
    35761 ggcgcaaacg ctggtcactc agacagcaga aacgctggca cagcatcaac aacaccgacc
    35821 tgacgggttg gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca
    35881 ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga
    35941 tgcagataac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa tgacgcagca
    36001 ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg
    36061 caagtttgcc caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac
    36121 ccggctgcac gtgcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt
    36181 tgttgatacc tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag
    36241 tttcctcgtt agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg
    36301 tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac
    36361 cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca
    36421 cgtagaagcg atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct
    36481 gggctacagc aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa
    36541 tacagagggg tgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta
    36601 ttggctacgg tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt
    36661 ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat tatgagcgtg
    36721 ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg
    36781 tattatgcac tggtggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac
    36841 tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc
    36901 acgctctctt cgtcttacct gatgctcgcc attggtcggc tggtatccgg ctttccgcat
    36961 ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc
    37021 accgccgccg tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg
    37081 ctgggaacgt atttaagtca ggaatgctgg cgttacacct ttttattgat cgctgttttt
    37141 aatattgcgg tgatggcatc ggtctatttt tgggtgccag atattcgcga cgaggcgaaa
    37201 ggaaatctgc gcgaacaatt tcactttttg cgcagcccgg ccccgtggtt aattttcgcc
    37261 gccacgatgt ttggcaacgc aggtgtgttt gcctggttca gctacgtaaa gccatacatg
    37321 atgtttattt ccggtttttc ggaaacggcg atgaccttta ttatgatgtt agttgggcta
    37381 gggatggtgc tgggaaatat gctaagtggc aggatttcag gacgttattc accactgcgc
    37441 attgcagcag tgactgactt tatcattgta ctggcactgc tgatgctctt tttctgcggc
    37501 ggcatgaaaa caacgtcgct tatttttgct tttatttgtt gcgcgggatt atttgccctt
    37561 tcagcacctc tgcaaatatt gttactgcaa aatgccaaag gaggagagtt attaggtgcc
    37621 gcaggtgggc aaatagcgtt taacctcggt agcgccgtcg gcgcatattg cggaggtatg
    37681 atgctgacgc tggggctggc atataattac gtggcgctgc ctgccgccct gctttcgttt
    37741 gctgcgatgt catcgttgct gctgtatggt cgctataagc gccagcaagc ggcggatagt
    37801 ccggtgctgg cgaaaccact ggggtagatt actgtcacgg tgcgcgttgc ttattgccgg
    37861 atgcggcgtg aacgccttat ccgccctacg cggttctggc acattttgta ggcctgataa
    37921 gacgcggcaa gcgtcgcatc aggcatgatg cgccaattgc ctacgttttt tactcttgtg
    37981 gccataacca cgcagcgccg cgtacgccgc tggaatcacc gtgcttcgcc ttactcaccg
    38041 gcgtttcaca ttcgccgccg aagacaaatt gtttaatcaa ctgcccaacc gtttgatata
    38101 aacggtctac attgctcatc ccgcccccca ggacaatcac atccggatcg agaatattca
    38161 cgacatgtgc cagcgatttt gccagccgca gctcgtagcg acgcaatgcc agttccgcta
    38221 ccggatcgct ttcttcaacc aggcggataa tttcactgcc tttcagcgca tgtccgctca
    38281 aacgacgata atccgtcgcg aatcccgtgc ccgaaataaa ggtttcaata caaccttgtt
    38341 taccgcaata acaagggact tcctcgcgat aacgcagttc gtcttcgtcc atccacggta
    38401 gcggattgtg tccccactca cctgccgtgc cattgccgcc gatatgcgtc cgcccattga
    38461 atgccacgcc cgcgccgcat cccgtgccga taatcacggc aaataccgtc tgcgctcccg
    38521 ctgccgcgcc atctactgct tctgaaaccg ccagacagtt ggcatcattt gccagccgca
    38581 cttcccgctg caacctcgcg cttaagtctt tatcgaatgg ctgaccgttg agccaggttg
    38641 aattgacatt cttcaccaca ccggtgtagg gcgaaatcga acctggaatg cccataccta
    38701 ccgttccgcg ctgccctgtc gcctgctccg ccatatcaac caacgtggcg atcgtttcaa
    38761 tagtctgccg gtaatcatca cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg
    38821 catcgcccag tgcaatcact tcagttttgg tgccgcctaa atcgatacct atacgcactg
    38881 tactctcctt atttttttca atatcaatag cgtagagacg gacaaccgga ttggcaatgc
    38941 aaggccgccg acaattcgtt atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta
    39001 tcatgctgtg gttcaaaaat ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg
    39061 cagaagagat ggaaaaacag ctagcctcga tggcatttac cccatgcggc agccaggaca
    39121 tggcgaagat gggctgggtt cctccgatgg gatcgcacag cgatgcgtta acgcacgttg
    39181 ccaatggtca aattgttatc tgcgcgcgca aagaagaaaa aatcctcccg tctccggtga
    39241 ttaaacaggc gctggaagcg aaaatcgcca aactggaagc ggaacaggcg cgtaagctga
    39301 agaaaaccga aaaagattcg ctgaaagacg aagtgctgca ctctctgctg ccgcgtgctt
    39361 tcagccgttt tagccagaca atgatgtggc tcgacacggt taacggtttg attatggtgg
    39421 actgcgccag tgccaaaaaa gcggaagata cgctggcatt actgcgtaaa agcctggggt
    39481 cgttaccggt tgtaccattg agcatggaaa acccgattga actgacgctg accgaatggg
    39541 ttcgctccgg tagtgcggca cagggcttcc agctgcttga tgaagccgag ctgaaatcgt
    39601 tgctggaaga tggcggcgtg atccgcgcga agaaacaaga tctgaccagc gaagagatca
    39661 ccaatcacat tgaagccgga aaagtggtga ctaaactggc gctcgactgg cagcagcgca
    39721 ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct taagttctgc gacgagctgc
    39781 gcgatcaaaa cgaagatatc gaccgtgaag atttcgccca gcgttttgat gccgatttca
    39841 tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg
    39901 aagcacaacg ttaattgctg attttccttt aatgccggat gcgacgcctg ccgcgtctta
    39961 tccggcgtac gaagccacac caggcatata attattcgct acggcgagca ataattttta
    40021 gcgcagcaat attatgcgtt ttacgccgta actgatctaa tcgcctcatc cctattcgcc
    40081 tgtcagcagc acgcagaata ttcactaacc cccatttatc gctggtaaat tgttttgtta
    40141 acagctccgc ttttggggta tcaatatatt cacgcaataa atcagaaatt gaacaaatat
    40201 ccgtcaggta aggataccag caagtttcac cgtgaaaatt acgcactcca gcgttacctg
    40261 caataaagtc tttcgggtaa tcccagatca cgtttttatc caacgtgatc caataacgcg
    40321 gtaaatcggt acttccacca ttttggctgc gcatgggata acgggtacag tgaatttgca
    40381 ggttgatcgt tggcgtcagt aagtcataga tctcccgctg taacttgctc catggacgtt
    40441 gtgtcattgt ttttcctcaa gccggtgacg cgcttcactg cgaattttcc gcgttgcccc
    40501 ggttttcact aaatcacgta actgcgaaag atcgggttgt tcgacaaata agagtaatga
    40561 tggatcctct tttaaccacg ccgttttcac atcggcagcc agctgtggat tattaatagc
    40621 cagcgaccga tcctgaggct cagtcaatgt cgttaacaac gacgccgggg tatgtgcgtt
    40681 gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc agcaatgcac gccaccattc
    40741 atcatctgat ttatctttta tcgtagccag ttccgcaaaa aatacctgga gatcttcact
    40801 gatatttata tcaaccttga tgaattatga atatatcgga aattgaaaaa atcgcagcaa
    40861 aggaaatatt attatgggtt aaagagagga tatttcagaa aaatgccctc ccgttcaagg
    40921 ggaaggcgag gaattacaga tagcggcaca gataagaggt aggttcggca acttgaagat
    40981 gaaactcact gtgaccggga acattaaaca ccgaaccggc ttcatacact tgccagtcgg
    41041 tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag
    41101 cggtgctgaa ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc
    41161 tgctggaaaa gccgatcgat ttcactttgc cggaaaagta ctcattactt tgaagcataa
    41221 actggccctg tctggtagtt gaacatctac ttcactatag gggccagagg cgctggctgt
    41281 cacgcaaaat tacaccagta attccgcagc cagccgtgca atcaatacgt tagagagcaa
    41341 gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca
    41401 atccagcatg atgacatcag ccccttttgc cagtaattct ttcccagcat caatgatttt
    41461 ttgttctgaa tcatgaatgg ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg
    41521 ccatttttgc gcctgaacgg gcagcatctc ctcaaccgga acgataaccc ccacctgatg
    41581 atcttcaaca atagaggaaa ccagtggagg caatattcgc gacggctcaa gaaagatcgt
    41641 attacgcgca gtcatactac taatgtttgc tgtactcatt aataaaatga cgtcataacc
    41701 ctgattatcg agcacttcaa ccacaccttg cagatctcgc tccactttac gacgcgaaac
    41761 atgggccagc tggttgtcat ttaataatgt gagaatggtg tcttcgcctg cttctggcgc
    41821 gtactccgcc atcacttctt cacgacttaa cttccccagc aggctatgat gggaaatatt
    41881 atcttcgtca atgtattccg tcaggagcgg caaaacctcc tgcataggta caatgccgat
    41941 ggtcaggatc gccaacgacg cactcattgt tcatccacct tacttttctt tcgtcgttaa
    42001 ttaccgggca agtgtgaagc accactgctg acacttactt gtgctacaaa tgacaaaaag
    42061 cgtagcagca gacgtgcacg gcataatgta aagattccaa atgattccag taatggaatg
    42121 ttattgttta atattctaat tattagaaaa acataaatta tgaaaaatgt gacgcagatc
    42181 ccttgtagtg gcactcagaa tcccttccga gcagtctgat gcggtcactc tttgtcaggg
    42241 tcctcatagc gttttttcgc atccatcgct tcctgagccg tcggatgttc actgatcaac
    42301 gagtctggtt taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt
    42361 cctttttcaa tcgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt
    42421 cttccttatt tcacgggatg aacgttaagt ataggcgctc taaaatcaac aattgatcgt
    42481 ctgtgccagg gcgctacgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat
    42541 gataatatgc gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc
    42601 ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag
    42661 tcggttaacc aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat
    42721 gaagtgacga ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgc
    42781 cgcagttacc gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct
    42841 ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac
    42901 aaaccgacgg ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg
    42961 aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg
    43021 gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt
    43081 aaagaaaaaa ttccacttaa agtaaaaatc tcaatacatc ccttactata ccaataaata
    43141 ttcaagaatg aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc
    43201 caccacttct ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt
    43261 ccgccggtct ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc
    43321 tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcgaat taagcgccgc tgcgcctttc
    43381 tcgatggctt cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac
    43441 tgccggacaa tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata
    43501 aatcgcgatg gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata
    43561 cgttccgccg cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc
    43621 caaattgtca taaagtcatc ctttgttagg taatcctcta ttgtgtcgcg cttttgcctt
    43681 ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag
    43741 gagtgaggca atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat
    43801 tctcggcggt ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatacacccc
    43861 ctccccggat aaagtcgccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc
    43921 gcaagaagtg gcgcaaatcg ccgacatcat ttttgctgcc gttaaacctg gcatcatgat
    43981 taaagtgctt agcgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc
    44041 tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg
    44101 cgccatgccg aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc
    44161 gctggtaacc ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc
    44221 ggaagtaatt gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc
    44281 ctacgtattt atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg
    44341 cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga
    44401 aacgggagaa catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat
    44461 tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac
    44521 gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag
    44581 gccgccactt cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc
    44641 gatttcaacc actcacgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta
    44701 atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc
    44761 cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg acatgattac tgcaaactca
    44821 tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta
    44881 agcgccacaa tcgcttcatc gcccacatca tgaccccagg tatcgttgat gctcttgaaa
    44941 tggtcgatat cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat
    45001 tcattgcgta acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcga
    45061 gtactcatga cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg
    45121 acccagccaa acatcagagg ataaacgaca ataatgggaa gggagagcca ccattccagc
    45181 ggagcactat tgaacgacac ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc
    45241 accatcaaca ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga
    45301 cacagaatca tcaacatagc ggtggaaggc agcacgttta cgcccattac gcctacccac
    45361 attcccgcta atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg
    45421 gccctgctcg ctatctgcca ggctaaatgc ggccagacga acgcccagcc gaccaacacc
    45481 agccaccacc agcccggcgg cgggtgcgaa accagcgttg aagcaatcgg taagaacatg
    45541 ccagccaggc caaccgcacg gggtagtctg acgcgacggg cgaagcgcag cccggaccgc
    45601 tgatgttcgt tttgaggagt taaaggaggt tcctccccat gcgccgccgc ttttttgaaa
    45661 aagttttcat cattcattat ttttgggaac attcagaaac aattttccca aattatagag
    45721 acggggctaa aggcagaagt atgaaatttc gggtgagcca ctttcgtagc tcacccgtta
    45781 gcact
//
LOCUS       DAJTSF010000022        45781 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000022 DAJTSF010000000
VERSION     DAJTSF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.022"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(8..487)
                     /locus_tag="OO335_001306"
     CDS             complement(8..487)
                     /locus_tag="OO335_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR6001763.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            complement(671..2089)
                     /gene="gltD"
                     /locus_tag="OO335_001307"
     CDS             complement(671..2089)
                     /gene="gltD"
                     /locus_tag="OO335_001307"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6001764.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(2102..6562)
                     /gene="gltB"
                     /locus_tag="OO335_001308"
     CDS             complement(2102..6562)
                     /gene="gltB"
                     /locus_tag="OO335_001308"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6001765.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            7237..8166
                     /locus_tag="OO335_001309"
     CDS             7237..8166
                     /locus_tag="OO335_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6001766.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            8262..10598
                     /gene="arcB"
                     /locus_tag="OO335_001310"
     CDS             8262..10598
                     /gene="arcB"
                     /locus_tag="OO335_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6001767.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            10828..11481
                     /gene="elbB"
                     /locus_tag="OO335_001311"
     CDS             10828..11481
                     /gene="elbB"
                     /locus_tag="OO335_001311"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6001768.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            11478..12205
                     /gene="mtgA"
                     /locus_tag="OO335_001312"
                     /pseudo
     CDS             11478..12205
                     /gene="mtgA"
                     /locus_tag="OO335_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(12202..12834)
                     /gene="yrbL"
                     /locus_tag="OO335_001313"
     CDS             complement(12202..12834)
                     /gene="yrbL"
                     /locus_tag="OO335_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6001769.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(13047..13319)
                     /gene="npr"
                     /locus_tag="OO335_001314"
     CDS             complement(13047..13319)
                     /gene="npr"
                     /locus_tag="OO335_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6001770.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            complement(13316..14170)
                     /gene="rapZ"
                     /locus_tag="OO335_001315"
     CDS             complement(13316..14170)
                     /gene="rapZ"
                     /locus_tag="OO335_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6001771.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            complement(14216..14707)
                     /gene="ptsN"
                     /locus_tag="OO335_001316"
     CDS             complement(14216..14707)
                     /gene="ptsN"
                     /locus_tag="OO335_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6001772.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            complement(14825..15112)
                     /gene="hpf"
                     /locus_tag="OO335_001317"
     CDS             complement(14825..15112)
                     /gene="hpf"
                     /locus_tag="OO335_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6001773.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            complement(15135..16568)
                     /gene="rpoN"
                     /locus_tag="OO335_001318"
     CDS             complement(15135..16568)
                     /gene="rpoN"
                     /locus_tag="OO335_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6001774.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            complement(16616..17341)
                     /gene="lptB"
                     /locus_tag="OO335_001319"
     CDS             complement(16616..17341)
                     /gene="lptB"
                     /locus_tag="OO335_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6001775.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            complement(17348..17905)
                     /gene="lptA"
                     /locus_tag="OO335_001320"
     CDS             complement(17348..17905)
                     /gene="lptA"
                     /locus_tag="OO335_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6001776.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            complement(17874..18449)
                     /gene="lptC"
                     /locus_tag="OO335_001321"
     CDS             complement(17874..18449)
                     /gene="lptC"
                     /locus_tag="OO335_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6001777.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            complement(18446..19012)
                     /gene="kdsC"
                     /locus_tag="OO335_001322"
     CDS             complement(18446..19012)
                     /gene="kdsC"
                     /locus_tag="OO335_001322"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6001778.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            complement(19033..20019)
                     /gene="kdsD"
                     /locus_tag="OO335_001323"
     CDS             complement(19033..20019)
                     /gene="kdsD"
                     /locus_tag="OO335_001323"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6001779.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            complement(20033..20998)
                     /locus_tag="OO335_001324"
     CDS             complement(20033..20998)
                     /locus_tag="OO335_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6001780.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            21208..22017
                     /gene="mlaF"
                     /locus_tag="OO335_001325"
     CDS             21208..22017
                     /gene="mlaF"
                     /locus_tag="OO335_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6001781.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            22025..22807
                     /gene="mlaE"
                     /locus_tag="OO335_001326"
     CDS             22025..22807
                     /gene="mlaE"
                     /locus_tag="OO335_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6001782.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            22812..23363
                     /gene="mlaD"
                     /locus_tag="OO335_001327"
     CDS             22812..23363
                     /gene="mlaD"
                     /locus_tag="OO335_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6001783.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            23382..24017
                     /gene="mlaC"
                     /locus_tag="OO335_001328"
     CDS             23382..24017
                     /gene="mlaC"
                     /locus_tag="OO335_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6001784.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            24017..24310
                     /gene="mlaB"
                     /locus_tag="OO335_001329"
     CDS             24017..24310
                     /gene="mlaB"
                     /locus_tag="OO335_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6001785.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            24470..24724
                     /gene="ibaG"
                     /locus_tag="OO335_001330"
     CDS             24470..24724
                     /gene="ibaG"
                     /locus_tag="OO335_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6001786.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            24779..26038
                     /gene="murA"
                     /locus_tag="OO335_001331"
     CDS             24779..26038
                     /gene="murA"
                     /locus_tag="OO335_001331"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6001787.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(26086..26364)
                     /gene="sfsB"
                     /locus_tag="OO335_001332"
     CDS             complement(26086..26364)
                     /gene="sfsB"
                     /locus_tag="OO335_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6001788.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(26591..27562)
                     /gene="ispB"
                     /locus_tag="OO335_001333"
     CDS             complement(26591..27562)
                     /gene="ispB"
                     /locus_tag="OO335_001333"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6001789.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            27820..28131
                     /gene="rplU"
                     /locus_tag="OO335_001334"
     CDS             27820..28131
                     /gene="rplU"
                     /locus_tag="OO335_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6001790.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            28152..28409
                     /gene="rpmA"
                     /locus_tag="OO335_001335"
     CDS             28152..28409
                     /gene="rpmA"
                     /locus_tag="OO335_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6001791.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            28536..29501
                     /locus_tag="OO335_001336"
     CDS             28536..29501
                     /locus_tag="OO335_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6001792.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            29517..30695
                     /gene="cgtA"
                     /locus_tag="OO335_001337"
     CDS             29517..30695
                     /gene="cgtA"
                     /locus_tag="OO335_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6001793.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFI
                     IENPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR
                     "
     gene            complement(30881..32314)
                     /gene="dacB"
                     /locus_tag="OO335_001338"
     CDS             complement(30881..32314)
                     /gene="dacB"
                     /locus_tag="OO335_001338"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6001794.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            32562..33038
                     /gene="greA"
                     /locus_tag="OO335_001339"
     CDS             32562..33038
                     /gene="greA"
                     /locus_tag="OO335_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6001795.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            complement(33194..33487)
                     /gene="yhbY"
                     /locus_tag="OO335_001340"
     CDS             complement(33194..33487)
                     /gene="yhbY"
                     /locus_tag="OO335_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6001796.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            33613..34242
                     /gene="rlmE"
                     /locus_tag="OO335_001341"
     CDS             33613..34242
                     /gene="rlmE"
                     /locus_tag="OO335_001341"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6001797.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            34342..36276
                     /gene="ftsH"
                     /locus_tag="OO335_001342"
     CDS             34342..36276
                     /gene="ftsH"
                     /locus_tag="OO335_001342"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6001798.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            36366..37214
                     /gene="folP"
                     /locus_tag="OO335_001343"
     CDS             36366..37214
                     /gene="folP"
                     /locus_tag="OO335_001343"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6001799.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            37207..38544
                     /gene="glmM"
                     /locus_tag="OO335_001344"
     CDS             37207..38544
                     /gene="glmM"
                     /locus_tag="OO335_001344"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6001800.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            38772..39104
                     /gene="secG"
                     /locus_tag="OO335_001345"
     CDS             38772..39104
                     /gene="secG"
                     /locus_tag="OO335_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6001801.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            39119..39205
                     /locus_tag="OO335_001346"
     tRNA            39119..39205
                     /locus_tag="OO335_001346"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39153..39155,aa:Leu,seq:gag)
     gene            39683..41290
                     /locus_tag="OO335_001347"
                     /pseudo
     CDS             39683..41290
                     /locus_tag="OO335_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(41298..42641)
                     /gene="argG"
                     /locus_tag="OO335_001348"
     CDS             complement(41298..42641)
                     /gene="argG"
                     /locus_tag="OO335_001348"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6001802.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            42989..43065
                     /locus_tag="OO335_001349"
     tRNA            42989..43065
                     /locus_tag="OO335_001349"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43023..43025,aa:Met,seq:cat)
     gene            43272..43724
                     /gene="rimP"
                     /locus_tag="OO335_001350"
     CDS             43272..43724
                     /gene="rimP"
                     /locus_tag="OO335_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6001803.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            43752..45239
                     /gene="nusA"
                     /locus_tag="OO335_001351"
     CDS             43752..45239
                     /gene="nusA"
                     /locus_tag="OO335_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6001804.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            45264..>45781
                     /locus_tag="OO335_001352"
     CDS             45264..>45781
                     /locus_tag="OO335_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 associated
                     domain-containing protein"
                     /protein_id="HCR6001805.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAA"
BASE COUNT        10933 a        11242 c        12297 g        11309 t
ORIGIN      
        1 ccttcgttta tttgcatgaa tatacaaacc gaaaatagga gatgcaatac aaaagctgtg
       61 gaaatatcaa acagcctctc aatttggcag acagcgtctg ccaaatcttt gccgatggga
      121 attctgcaga tggtgtctgc ggaattccca atggtgttaa ttcgccagac actgtctggc
      181 gaatttcaac atgttgaaaa aagaggtaaa aaagcctcat ttggcgtaag ttttttgcag
      241 agaccccacg tttataccgt cgacttaaat ccttcgatag ccgatcaatc aactgtgcac
      301 cataagctgc tctggcctca ccaccttgtt caaattcgac aatccgtcgg ccaatttccc
      361 agtacgtcgc ggtcatcagc gcgttaacgc tacgtaccgt ttccgttcgg gcgctatcga
      421 ccagatgaat aatgccgttg tggatttgct ggtagcctgt tgaggttctc tctgagagcg
      481 attccattat ctgtcccttt atatggcgct gcgttgaata atggaatcag ctatacgccg
      541 ggcatctacc ggtaacaatc cagatgagcg tattacattg gttttcttca gattatgcga
      601 ggcgatttcc agaaagaaag gagaatttca gcgccgactc ttttctgagg aggcggcgct
      661 gggacagagc ttaaacttcc agccagttca taatgccgtc tgccgctttg cgaccttcgg
      721 cgatggcggt aacgaccaga tcggaaccac ggacgatatc gccgccagca aagattttcg
      781 ggttgctggt ctggaaagcg ttgtcgttgc cttccggagc gatgatgcgg ccttgtgaat
      841 ccagctcgac gctgtgtttt gccagccatt ccatgttgtg tggacggaaa ccaaacgcca
      901 tgatcaccgc atctgccgga acgatatgtt cggaacctgc aacgatctcc gcgcggcgac
      961 ggcctttggc atccggttcg cccatttcgg tacgtaccat ttttacgccg ctgactttgc
     1021 cgttaccgtt cacttcaatc cccagtggct ggacgttgaa tttgaactct acgccttctt
     1081 cccgcgcgtt tttcacttcg cggcgggaac ccggcatgtt ctcttcatca cgacgatagg
     1141 cacaggtaac gtgctttgcc ccctggcgta cagacgtgcg cacgcagtcc atcgcagtgt
     1201 cgccaccgcc aagtactacc acgcgtttgc cttccatgct gacgaacggt tcgtcgcggg
     1261 tttcaccaaa gcccattaac tgtttggtgt tggcgataag gaacggcagc gctgcgtaca
     1321 cgccatcggc gtcttcgttt tccagcccgc cgcgcattga ctgataagtc ccaacgccaa
     1381 ggaacacggc atcgtaatca ctcagcagat cgtccagctg tacgtcgcgg cccacttcgg
     1441 tattgagttt gaattcaata cccatgccgg tgaagatttc acggcgacgc gtcattacct
     1501 ctttttccag cttgaaggcc ggaataccga aggtcagcag cccgccaatt tctggatgac
     1561 ggtcgaagac aacggctttt acgccgttac gcgtcaggac atccgcacac gccagacctg
     1621 ccgggcctgc accgataatc gccacttttt taccggtttg cttcacgccg gacatatccg
     1681 gacgccagcc catttcgaac gctttatcat tgatataacg ctcaatgttg ccgatggtca
     1741 ccgcaccgaa ctcatcgttc agggtgcagg aaccttcgca cagacggtct tgcgggcaga
     1801 cacgtccgca tacttccggc agtgtgttgg tctgatgcga cagttctgcc gcttcaaaaa
     1861 tacgcccctc gttggcgagc ttcagccagt ttgggatgta gttgtgtacc gggcatttcc
     1921 actcgcagta tgggttgccg cacgacagac agcggtcagc ctgcgctttg gcctggcctt
     1981 cggaaaacgg ctcgtaaatt tcaacaaact caattttacg gatcttcagc ggtttctttg
     2041 gcggatcaac gcgctgcagg tcgataaatt gataaacatt ctgactcatt gttgctaccc
     2101 cttactgcgc ctgcacgcgc aactctgctg cgctacgact acggtgaccc agcagtgctt
     2161 ttacatcact ggacttcggt ttaaccagcg cgaatttcgt ggcgaaggtt gaccagttcg
     2221 ccagaatctc ttcaccgcgc tgagaaccgg tatgctgaac atgctcggtg ataagaccgc
     2281 gcagatgctc ttcatggatc gccagatcgt cgacgcttaa gacttcgacc agttccgggt
     2341 taacgcgttt gcggaaatca ccgctttcat cgagaacgta agcgaagccg ccggtcatgc
     2401 ccgcgccgaa gttaacgccg gttttaccca gaatacagac aataccgccc gtcatatatt
     2461 cacagccgtt gtcgccaatg ccttctacca cggtgatagc accggagttc cgcacgccga
     2521 aacgttcacc cgcgcggcct gcggcataca gacgaccacc ggtcgcgcca tacaggcagg
     2581 tgttgccgat aatgcttgct tcatggctgc ggaaggcgga accaaccgga ggacgaatgg
     2641 cgattaagcc gcccgccatg cctttaccga catagtcgtt ggcatccccg gtcaggtaca
     2701 gttccacgcc gcccgcgttc cacacaccga aactctggcc tgcggtgccg ttgaagtacg
     2761 ctttgatagg atcggctgcc agcccctgat cgccgtgcgt ctgggcgata tagcctgaaa
     2821 gcgacgcgcc gacagaacgg tcggtgttgc ggatatcgaa ccagaaggtt ttgctctggc
     2881 gttcatcgac aaacggtttc gcctgttgca gcaactgcgc gttcagtagg ccgttatcaa
     2941 acggcgggtt gttttcagtg cagtagagtg acttacctgc atgtggctcg gcagtctcca
     3001 gcagcttcga cagcgccagt ttctgctgtt tggcggtgaa accgtccagc tctttcagca
     3061 ggtcggtgcg accaatcaga tccaccagac gcgttactcc cagctgtgcc atcagctcgc
     3121 gggtttcacg ggcgataaac tcaaagtaat tagtcacctt gaacggcagg ccgtgatagt
     3181 gattcttacg cagtttgtca tcctgagttg ctacacccgt ggcgcagttg ttcaggtggc
     3241 aaatacgcag atatttacag ccgagcgcca ccattgggcc agtgccgaag ccgaagcttt
     3301 ctgcgccgag aatcgccgct ttgatgatat cgacacccgt tttcaggccg ccatcaacct
     3361 gcaaacgaat tttatgacgc aagccgttgg caaccagcgc ctgttgggtt tccaccagac
     3421 cgagttccca cgggcagcct gcatatttca ctgacgacag cgggcttgcg ccggtgccgc
     3481 cgtcatagcc cgcgatggtg atcaagtccg cataagcttt cgccacgcca gtcgcgatgg
     3541 tgccaacgcc tggttcggac accagcttaa cggagatcat cgctttcggg ttaacctgct
     3601 tgaggtcgaa aataagctgc gctaagtctt cgatagagta gatatcgtgg tgcggcggcg
     3661 gggagatcag cgtcactccg ggcaccgaat agcgcagttt ggcgatgtaa ggagtgactt
     3721 tgtcgcccgg caactgacca ccttcgcccg gcttcgcgcc ctgggcgact ttaatctgaa
     3781 tgacgtcggc attgaccaga tacgccggag taacgccgaa gcgaccagaa gccacctgct
     3841 tgatgcgcga cactttgtta gtgccatagc gcgccgggtc ttcgccgcct tcaccggagt
     3901 tcgagttacc gccgatgcta ttcatcgctt ccgccagcgc ctcgtgggct tccgggctta
     3961 acgcgccgat agacatcgcg gcggtatcaa agcgtttaaa cagttcgctt gccggttcaa
     4021 catcagcaat gttgaccgcg ttttcacccg gcgtaattgc cagcagatcg cgcagcgtgg
     4081 ttgccggacg ctcattaacc agcttcgcgt attcctgata gtcgctgtac tcgccgcttt
     4141 gtaccgcctg ttgcagcgtg cgcaccacgt ccgggttgta ggcgtggtat tcgccgccgt
     4201 ggacgtattt cagcaggccg ccctggctaa tcggcttgcg cgccagccag gcacgtttcg
     4261 acagattcag cagatcctgc tggaagtctt caaagcttgc cccaccaatg cggctgaccg
     4321 cgccctggaa gcacaggccc actacatcat cgtgtagacc gaccgcttca aacagtttcg
     4381 agcagcggta agaggcgatg gtggagatgc ccattttgga catgattttg tacaagcctt
     4441 tgttgatgcc gttacggtag ttgagcatca cggtacgata atctttggca atcgcgtggg
     4501 tgtctaccag gcggcccagc gtttcatagg caaggtacgg ataaatagcc gtcgcgccga
     4561 aacccagcaa cacggcgaag tggtgcggat cgcgggcgct ggcggtttcg acgatgatgt
     4621 tggcatcgca acgcaggctt tgatcgacca gacgggtctg gatcgcgcca accgccatcg
     4681 gagccggaac cggcaggcga tctttagcga tattccggtc ggagagcacc agcaacacgg
     4741 tgccgctacg taccattttt tcggctttgt cgcatagctc tttgactgtc gcttcgagcg
     4801 tggttttagt gacgtcaaag gtgatatcca gcgtatctgc gcggtagtgc tcctctttca
     4861 tcgtcgtgag ctgtttgaaa tcggagtaga gcagaatcgg cgatttaaag cttaaacggt
     4921 gcgcctggcc ctctgcttcg caaaagacgt tcatttcacg accgatactg gtggcgagcg
     4981 acataacatg cgcttcacgc agcgggtcga ttggcgggtt agtcacctgg gcaaactgct
     5041 ggcggaagta gtcgtaaata atgcgcggct gactggagag cacggcgaat ggggtatcat
     5101 cgcccatcga accgaccgct tcctgaccgt tttcgcccag tacgcgaatt acggagtcca
     5161 gctcttccgc gctgtagtta aactgtttct ggtagctggc aagcgtgtcg tcgtccagtt
     5221 cgcggctacc cacttcttca tcgggcagat cttcaaacgg taccagtcgg cggacgtttt
     5281 tctccatcca ctctttataa gggtggcggc ttttcagatc gtcatcggtt tctgccgagt
     5341 gcagaatacg cccactgcga gtgtcgatca ccatcagttc gcctggcccg acgcggcctt
     5401 tttcgaccac ttcgtcaggc tggtaatccc agataccgac ttcagaggcg caggtgatga
     5461 gcttgtcttt ggtaatgacg tagcgtgccg gacgcagacc gttacggtcg aggttacagg
     5521 cagcgaagcg accatcggac atcacgatgc ccgccggacc atcccacggc tccatatgca
     5581 tggagttaaa gtcaaagaag gcacgcagtt ccgggtccat atccgggttg ttctgccagg
     5641 cgggtggcac taacaggcgc atggcgcgga tgatatccat cccgcctgcc agcagcagtt
     5701 ccagcatgtt atccatggaa ctggagtcag aaccggtttc gttgacgaat ggcgcggcgt
     5761 cgtgcaggtc agggatcagc ggcgtctgga atttataggt acgcgcacgc gcccattggc
     5821 ggttaccggt gatggtgttg atttcaccgt tatgcgccag atagcggaac ggttgcgcca
     5881 gcggccagcg cggtacggtg ttagtggaga agcgctggtg gaacaggcaa atggccgatt
     5941 ccagacgcag gtccgcaaga tccagataaa agcgcggcag atccgccggc atacacagac
     6001 ctttatagat gttcaccaga ttcgacaggc tacagacgta gaagtctttg tcggcttcga
     6061 gacgcttttc aatgcggcgg cgggcgataa acagacggcg ctccatatcg cgtggacgcc
     6121 agcctgccgg ggcgttcaca aaaatttgct caatgcgtgg cagagaggag agggcgattt
     6181 cacccagcac gccttcgtta gtggggacat cacgccagcc cacaatcgac aaggtttcgc
     6241 gttgcagttc ttcttcaacg atgcggcgtg cggcagcggc gagttcagga tctttattca
     6301 ggaagagcat cccgacagcg tagttttttg ctaaacgcca gccgcgctcc tgcgcaacga
     6361 tgcgaaaaaa gcgatccggt ttttgtaaca gcaagccgca accgtcgccg gttttaccat
     6421 cggcgagaat cgcgccacgg tgctgcatgc gggccagtgc gtgtattgca gtacgcacta
     6481 ccttgtggct aggttcgcct tctatgtggg cgatcaggcc gaaaccacag ttatccctct
     6541 caagggattt atcgtacaac atatcgtgaa cctcccccag gctctgcggg aaccccactt
     6601 ccgaaccgct gcgcacgggc acagaaagag cgtggcgacg gggtttgcgt gtcatacgcg
     6661 cgcctcgcat tcgccccctt gttgtccttt tcgcatcggt taatacggtg aagaggggat
     6721 ctcaattact gcataaatat gatgagcagg ctgctcatcc agaaagcttc cagcggattt
     6781 ccaacttatc gggaaagctg atacaggtca aatggcaagc ttattggtac agaaatgtgc
     6841 tttagcgcaa aattctttat taaatagctg aaattaaaat agattttatc tttaaggcta
     6901 aaaggagagt cgttagagaa acagtcaatt cctgactgtc ataaaggcgt gttaaccctt
     6961 atcctgcctt gaactgacga gaaaaccagc attttatact gccttaattg gtaatgacgt
     7021 ttcatgctac tgtttttgcc taaaatccat aaggagataa aaaaatctga cacaagataa
     7081 gcaaaagtga tttatgtgat tgtgcatgaa atttcggtaa ttttgattga ttatgcagtt
     7141 gataaaagca ggccgggacg attgatccag gtcatcgccg aattgagcta aaaatggcag
     7201 gctttggcct cttttcaagg cgcggtctat cagattatgc agttacagaa attagtcaat
     7261 atgtttggtg gtgatctcac ccgtcgttat gggcaaaagg tgcataagct gacgctgcac
     7321 ggcggtttta gctgccctaa ccgtgacggt accatcgggc gtggtggctg cacattctgt
     7381 aatgttgcct cgtttgccga tgaagcgcag cagcatcgtt ccattgccga gcaactggcg
     7441 caccaggcga atttagttaa ccgcgctaaa cgctatctgg cctactttca ggcgtatacc
     7501 agcacctttg cggaagttca ggtgctgcgt tctatgtatc agcaggcggt gagccaggcc
     7561 aatattgtcg gtttgtgtgt tggtacccgc ccggactgcg tgccggatgc ggtgctggat
     7621 ctgctttgcg aatataagga ccagggttac gaagtgtggc tggagctggg gctacaaacc
     7681 gcccacgaca aaacactgca tcgcatcaac cgcggccatg attttgcctg ttatcagcgt
     7741 acaacccagc tggcacgtca gcgcgggctg aaggtgtgtt cccacttaat tgtcggcctg
     7801 cctggcgaag ggcaggctga atgtttgcaa acgctggagc gagtggttga aaccagcgtg
     7861 gacggcataa agctgcatcc gctgcatatt gtgaaaggca gcattatggc gaaagcctgg
     7921 gaagcggggc gtttgaacgg tattgaactg gaggattata cgctcactgc cggagaaatg
     7981 attcgccata cgccaccgga agtgatctac caccgcattt ccgccagcgc ccgtcgcccg
     8041 acgctgcttg ctccgctgtg gtgcgaaaat cgatggacgg gaatggtcga gctggatcgc
     8101 tatctgaatg aacatggcgt acagggatcg gcgctgggac gtccgtggct acctccaacg
     8161 gagtaggtcg ttgaggggaa ttccgcattt ctcacacaat ttataacgta actgtcagaa
     8221 ttgggtatta ttggggcagg ttgtcgtgaa ggaattccct aatgaagcaa attcgtctgc
     8281 tggcgcagta ttatgttgac ctgatgatga agttaggtct ggtgcgcttc tcaatgttgc
     8341 tggcgctggc cctcgtcgtt cttgccattg tggtacaaat ggcggtaacc atggtgctgc
     8401 atggtcaggt cgaaagcatt gatgttattc gttctatctt ctttggtttg ctgattacgc
     8461 cgtgggcggt ctactttcta tcggtggtcg tcgagcaact ggaggagtca cgacaacgtc
     8521 tgtcacggct ggtgcaaaaa ctggaggaga tgcgcgagcg cgatttgagc ctcaacgttc
     8581 agttaaaaga taatattgcc cagctaaatc aggaaattgc cgttcgtgaa aaagcggaag
     8641 cagaactgca ggaaaccttc ggccaactga aaattgaaat caaagagcgc gaagagacac
     8701 aaattcagct cgagcagcaa tcctcattct tacgttcctt ccttgatgct tcacccgact
     8761 tggtttttta tcgtaacgaa gataaagagt tttccggctg taaccgcgcg atggagctgc
     8821 tgaccggaaa aagcgaaaaa caactggttc acctgaaacc tgctgatgtt tactcaccgg
     8881 aagccgccgc aaaagtcatt gaaaccgatg aaaaagtgtt ccgtcataat gtgtcactga
     8941 cctatgaaca gtggctggat tacccggacg ggcgcaaagc ctgctttgaa atccgtaaag
     9001 tgccgtacta cgaccgcgtg ggtaaacgtc acggtttgat gggctttggt cgcgacatta
     9061 ccgagcgtaa gcggtatcag gatgcgcttg aacgcgccag ccgcgacaaa acgacgttta
     9121 tttccaccat cagtcacgaa ttgcgtacac cgctgaacgg tatcgtcggc ctgagccgca
     9181 ttctgctgga taccgaactc accgccgagc aggaaaaata tctcaaaact atccatgttt
     9241 cggccgtcac gctggggaat atctttaacg atattatcga catggataag atggaacggc
     9301 gcaaggtcca gcttgataat cagccggttg atttcaccag cttccttgcc gatctggaaa
     9361 atctctccgc attgcaggcg caacaaaaag gattgcgctt taacctggag cctacgctgc
     9421 cattaccgca tcaggtcatt accgacggga cgcgtttacg gcagatcctg tggaacctca
     9481 tcagtaacgc cgtcaaattc acccagcaag gccaggttac cgtgcgcgtg cgctacgatg
     9541 aaggcgatat gctgcatttt gaagtggaag attctggtat cggcattccg caggatgagc
     9601 tggataaaat cttcgccatg tattaccagg tgaaagacag tcatggcggt aaacctgcca
     9661 ccggcaccgg tattggtctg gccgtttctc gtcgtctggc gaaaaatatg ggcggcgata
     9721 ttacggttac cagtgaacag ggcaaaggtt caacctttac gttgacgatc cacgcaccgt
     9781 cggtggcaga agaggtcgat gatgcgtttg atgaagacga tatgccttta ccggcgctga
     9841 atgtgctgct ggtggaagac attgaactga acgtgattgt cgcgcgttct gtgctggaaa
     9901 aattaggtaa cagcgttgat gtcgccatga ccggcaaggc ggcgctggag atgtttaaac
     9961 cgggcgaata cgacctggtg ttgctggata ttcagttgcc agatatgacc gggctggata
    10021 tctctcgtga actgacgaag cgttatccgc gcgaggattt accgccgctg gtggccttaa
    10081 ccgctaacgt gctgaaagac aaacaagagt acctcaatgc tggaatggat gatgtgctga
    10141 gtaagccgct ttcggttccg gcgctaaccg cgatgatcaa gaaattctgg gatacccagg
    10201 atgatgagga gagtacggtg acgacagaag agaacagtaa atcagaagca ttgctcgata
    10261 ttcccatgct ggaacagtat ctcgaacttg taggaccgaa gctgatcacc gacgggttag
    10321 cggtgtttga gaagatgatg ccgggctatg tcagcgtgct ggagtcgaat ctgacggcgc
    10381 aggataaaaa aggcattgtt gaggaaggac ataaaattaa aggtgcggcg gggtcagtgg
    10441 ggttacgcca tctgcaacag ctgggtcagc aaattcagtc tcctgacctt cccgcctggg
    10501 aagataacgt cggtgaatgg attgaagaga tgaaagaaga gtggcgtcac gacgtagaag
    10561 tgctgaaagc gtgggtggca aaagccacta aaaaatgacc ccggctagac cggggtgcgc
    10621 gaatactgcg ccaacaccag ggaaatcttg gctgcgccgt aaattattat gatgagttac
    10681 aagggcacag cactgttttt caggccgcac gcagttaaga tagcaaaact taaatgattt
    10741 gttacatgaa tcagttaaat gtgtgatgcg taccattttt attaaaaaaa ttaatgtgtt
    10801 tcagtcaatt gcgaaaggat gatcacaatg aagaaaattg gcgtaattct gagcggatgc
    10861 ggcgtctatg acggttctga aattcatgaa gcggtgttga cgttgttagc tatttcacgc
    10921 agcggtgcgc aggcggtctg ctttgcaccg gataagcagc aggttgatgt tatcaaccat
    10981 ttaactggcg aagcgatgac ggaaacgcgc aatgtgctga ttgaagcggc acgaataacg
    11041 cgcggtgaaa tccgtcctct ggcccaggcc gatgccgctg aactggatgc gttgattgtg
    11101 ccgggggggt ttggcgcggc gaagaattta agcaattttg ccagtcttgg tagcgaatgc
    11161 accgttgacc gtgaattaaa ggcgctggca caagcgatgc atcaggccgg aaaaccgctt
    11221 ggttttatgt gtattgcccc ggcgatgctg ccgaaaattt tcgatttccc gctgcgtttg
    11281 accatcggta ctgatatcga taccgcagaa gtgctggaag agatgggcgc ggagcatgtg
    11341 ccgtgtcctg tcgatgatat cgtggttgat gaagacaata agattgtcac caccccagca
    11401 tatatgctgg cgcagaacat tgcagaagcg gcgagcggca ttgataagct ggtttcccgc
    11461 gtgctggttc tggctgaatg agtaaaagcc gcttaacggt gtttagtttc gttcgccgtt
    11521 ttcttttgcg gttaatggtt gtcctcgccg ttttctgggg cgggggcatc gcgttgttta
    11581 gcgttgcgcc tgttcccttc tcagcggtaa tggtcgagcg acaggtcagc gcctggctgc
    11641 atggcaattt tcgttacgtg gcacattctg actgggtcag tatggatcaa atctcgccgt
    11701 ggatgggact ggcggtgatt gccgcagaag atcagaaatt tcctgagcac tggggctttg
    11761 atgtcgcttc cattgagaaa gccctggcgc acaacgagcg caatgaaaac cgtattcgcg
    11821 gtgcttcaac gatttctcag cagacggcca aaatctcttt ttatgggatg ggcgtagctg
    11881 ggtacgaaaa gggctggaag ctggattaac gctggggata gaaacagtct ggagcaaaaa
    11941 gcgtatcctg acggtttacc tgaatatcgc cgaatttggc gacggtgtgt ttggcgtcga
    12001 agctgcggca caacgttatt tccacaaacc cgcgagcaaa cttacccggt cggaagctgc
    12061 attactggca gctgtattac ctaatccact tcgtttcaaa gtctcctcgc catcgggcta
    12121 cgtgcgtagc cgtcaggcgt ggattttacg gcagatgtat cagttaggcg gtgagccgtt
    12181 tatgcaacaa caccagctgg attaagcttc gcgcgaagtg agggccagcg ttttgctctc
    12241 acgaggttgc agatctttct gcagggcaat agccagcgcc ggttgggcga taaatcgttt
    12301 ccataaccgc tcttgcttac gcagacaaca ccacttcgac caggtcgcca gtggaatcaa
    12361 tgtgctttca ccaatgttgt cgcagacgac cgggataacc tctgactcac tgatgcggtg
    12421 acagaggata ttctgcggct tcagcgacat cgtcacgata cggttatcct gcaaataacg
    12481 cttcagctgt ttcagtagct ggcgcagttg tgcaatgtct tcttcgtaac ggcattgttc
    12541 agcaaattcg gtcagcgtaa tggaaggctt gccgtcaaaa tcggcaatca catcgtagac
    12601 ataaccggtt ccgcaatccg tctcaacggt accgtgatag cgcggtattc cactccagtc
    12661 ttttaaccgg cgaccaagat gcgcgtagta ctttaactca cggcggatct ctttatcgcc
    12721 accatcgcca cgatggtaga caatcttgat acagcgttgg gcatcttccg gatgcgcata
    12781 acatttccga tgtcttccgg tgcccagggg actttgttca gataagcgaa tcatgccatc
    12841 tcctggaaat attttctgac gatctccagt atggtcgatt aacttaaaca aaacctaaac
    12901 aatgcctctt aaggttttct taaggttctt ctgaaagtga aaagcgcaca aaatgtacaa
    12961 atgacgttga ttgagaacga tttcagtacg tgattttctt attgtcgggg gagcttgaag
    13021 ggagttgtat gtcaaagtga tgaagattaa tcttcatcaa aaccagaatt aaagagggcg
    13081 ataacggcgg ccagtgcttc ctcttcctgt ggaccggtcg cttcaacttc aatctgccgt
    13141 cctttggcag aatccaacat cagcagcgca ataacgctgt tggcttcagc ctcggtgcct
    13201 tcgtcattac gtaacagcac ttcagcgtca aaaccctgca ttaattcaaa cagcttcatt
    13261 gcaggccggg catgcatgcc cagcttgttt gtgatttcaa cagtttgctt gacggtcatg
    13321 gtttacgttt ttccagcgta cgatggcgtg actggacgtt tttaccgcgc gagcggaagt
    13381 agtctgccag ttgctctgca atatacaccg aacggtgctt cccgccggta caaccaatgg
    13441 cgaccgtcag gtagctacgg ttgttggttt ccagcatagg taaccatagc tcaagatagc
    13501 ttcgcgtctg gtagataaaa ttgtgtactt ctgtgtggcg gtcgaggaac gcggcgacag
    13561 gtttatcaag acctgtcatt ggacgcagtt tcggatccca gtgcgggttc ggcaagaagc
    13621 gcacgtcaaa gacgtaatct gcatcgatag ggataccgtg tttgaagccg aaagactcaa
    13681 agaccatggt cagttcgcgt tcacgtttac ccagcagacg ggtacgcagc atttcagcca
    13741 gctcgtgaac ggacatttct gaggtatcga caatcagatc cgctcgcgaa cgcagaggct
    13801 ccagcaaatc gctttctttg tcgatagcac tttccagcga caggtttttg ctggaaagcg
    13861 gatgcagtcg gcgcgtgtca ctgtaacgac gaattaaggt attacggtcg gcatccagga
    13921 acagtagttg cggtgagaaa gcgtcaggca ggttactcat cgcctgttcg aatatttctg
    13981 gtgactccgg catattacga acatcaatgc tgacggcggc agaaatctcg cgatcggcca
    14041 gagttcgagc cagatcgggt aacaacacta cgggaaggtt atccacgcag taaaaaccca
    14101 tatcttccag cgcacgcagg gcgacagatt tacctgaacc tgaacgtccg ctgacgatca
    14161 tcagtaccat gtactgtttc tcctcacaac gtctaaaaga gacattaccg aataactacg
    14221 cttcatccgg agtaccttcg gtatccgtaa tgatttgata cagctcttca tcgctctggg
    14281 ctgcgcgtaa acggcggcag atggttttgt ccgccagacg tttcgccacc agcgacagag
    14341 tatgcaggtg cgttttagtt tggtctgccg gcaccagcag ggcaaaaagt aagtccaccg
    14401 gttggttgtc gatggcatcg aaagctatag gcgtttcgag ctgaacgaaa acgccaacgg
    14461 cacgcagagt atcttcttca agtttgccat gcggaatggc aataccattg ccgataccgg
    14521 tactgcccat tttttcccgc gtcaggatag cttcaaaaac cacctgaggc ggcaggctaa
    14581 gttgtttcgc cgccagctcg ctgatgattt ccagggcgcg ttttttgctc tgacagtgga
    14641 cgcggcttcg cgtacattcc ctgttaagaa cactgctaag ctgtagagtt gtatcattat
    14701 ttgtcataat ttcacctaag aacctgccca ctcaaacagt ggaacaggct cggagcatta
    14761 accgtacaaa tggcccgttg tgccgcacaa caggccgcca tgcacatgct aattgcccgg
    14821 acaattagtg ttgtttcagt ttatctttat gtttggtcaa ctggcgtgcc agcttatcaa
    14881 ttaagccatc aatggcagcg tacatgtcct gaccttccgc gctggcatga atttcaccac
    14941 cgtttacatg cagtgttgca tctgaggtgt gggtgacttt ctccactttc agaacaacat
    15001 agacctggtt gattcggtca aaatattgct caagtttggc aaatttagct gtaacaaatt
    15061 cgcgcagtgc ctcggtgatc tcgacgttat ttccggtaat gttgagctgc atagtgtctt
    15121 ccttatcggt tgggtcaaac gagctgttta cgctggtttg acggcggaat ggataaagac
    15181 tctcggtact tcgcaacagt gcggcgtgcc accatgatac cttgttccga cagcaaagag
    15241 gttaacttgc tgtcgctcaa cggtttcgct gggttttccg ccgcgattaa tttcttcacc
    15301 agtgcacgaa tcgccgtgga ggaagcttcg ccgccgccct cggtattgac gtgactggaa
    15361 aagaaatact tcagttcaaa aatgcctcgt ggactatgca ggtatttttg cgtggtcacg
    15421 cgagatatcg tcgattcatg catttcgaca gcctgggcga tatcggccag taccatcggt
    15481 ttcatatatt cttcaccttg ctcaaagaag gcttgctgct gttcaacgat acagcgactc
    15541 acgcgcagta gcgtatcgtt acggctttcc agactcttga tcaaccattt ggcatcctgc
    15601 agattgctgc ggataaactg gctgtcacca tcgttgcgcg cgttattgca catcgaggcg
    15661 tagtgctggt tgatttgcag acgcggaatg ctgtcactgt tgagttctac cgtccagtga
    15721 ccgttatgct tacgcaccag cacatctgga atgacatact caggttcgcc agtctggatc
    15781 gactgcccgg ggcgcggatc gagcgactgg atcagattga cggcttcttt cagcacatct
    15841 tctttcagac gcgtgacgcg cattaaagtg cggaagtcgt gattggctaa cagatcgaga
    15901 tgatcgctaa tgatcaatct ggcctcttcc agccacggcg tggtcttatc gaattgggag
    15961 agttggatta gcaggcagtc acgcagatct tttgccgcta caccgaccgg gtcaaaccgt
    16021 tggatccgct taaggacggc ttcaacctcg tcgatgtcga tctcttcatc gcccatactt
    16081 tcgagaatat cttccagcgg gacagtcaga taaccggttt catcaacggc atcgacgata
    16141 gaggtagcaa tagcgcggtc agtgtcggaa aacggtgtca gctcgacctg ccacatcagg
    16201 taatcctgca aggtctgcgt cgtttcgccc tggtagaccg gcagctcgtc gtcaatgtag
    16261 tcaccgctgg tgccggatgg tgtaccagcg gtgtaaatgg tgtcccaact ggcatcgagc
    16321 ggcagctctt ccggcatctc tttttgttcg agcgcgtcgg cggtgtccag cgtttcactg
    16381 tcttgcgttt cgcgggtgtc gatttcttca tgagtgtcga tttgctcaag cagcggatta
    16441 ctctccagcg cctgctgtag ctcctgctga agttccagcg tcgacaactg caacagacga
    16501 attgcctgtt ggagctgtgg cgtcatcgcc agttgttggc taagcctgag ttgcaaacct
    16561 tgcttcatgt tcagaatcgt actctcctgc taaaacgtcg caaacttcta ccctatcaga
    16621 gtctgaagtc ttccccaagg tatacacgct taacgtgttc gtcttgtaag atttctgtag
    16681 gcgtgccgtg ggcgatcaaa tgcccctgac tgacgatata agcgcgttca caaaccgcca
    16741 gtgtttcacg cacgttgtgg tcagtgatca gcacgcccag gccgctgtcg cgcaggtgtt
    16801 caatgatgcg tttaatgtcg ataaccgaga tcgggtcaac cccggcaaac ggttcgtcga
    16861 gcagaataaa tttcggattc gcagccagtg cgcgggcaat ttctacacgg cgacgttcac
    16921 ccccggagag tgactgcccc atgctgtcac gcaggtgctc aatgtgaaac tcttccatca
    16981 gctcgttcgc gcggtcttca cgttgttcag cagacaagtc gtcacgaatt tgcagtaccg
    17041 ccatcaggtt atcgtaaacg ctgaggcgac ggaaaatgga ggcttcctgt ggcagatagc
    17101 cgataccgcg gcgcgcgcgt gcatgcagag gcagcagact gatatcgtca tcatcaataa
    17161 tgatgttgcc cgcatcgcgc ggcacaatgc ctacaaccat gtagaaagtg gtggtcttac
    17221 cggcaccgtt tggccccagc agaccgacaa tttccccgga gttgacggtc aggctgacgt
    17281 cttctaccac gcgacggcct ttataggctt ttgcaaggtt ctttgcagtt aatgttgcca
    17341 taacgaatta attacccttc ttctgtgccg gggtctggcc tttgttgttt ttgtcctgca
    17401 gctgcgacgg caccagaacg gtggttacgc gcttgccttt gtcgctgaaa gcctgcattt
    17461 tctgctcttt caccaggtaa gtgatcttat cgcccttaat gttgctatcg acctgctgca
    17521 gataagcatt acccgtcaga acgacaaaat cttttgccag ttcgtagtgc atctgggaag
    17581 cgtgaccttc aacgggttta ccgttgtcct gcatctggta gaacgttgcc ggtttaccgt
    17641 atccgtcaat cacttcttta ccttgttcgc cgcccggacg ggtaacgacc actttgtcgg
    17701 cattaatttt gatggtgccc tgggtgacga tgacattacc ggtaaaggta accacgttgc
    17761 cttgcatatc aagagattgc tggtccgatt caatgtggat cggctgatca gtgtctccgg
    17821 ttacggcaaa tgccggaatg ctggcggcca gaagtgagct ggcaagcaca agattaaggc
    17881 tgagtttgtt tgttttgaat ttcataggat gttctaacct tttcaatcag ctcggcgttc
    17941 ttgctgcgta agttgccgcg cattttcaga ccgctggagt taaatgttgt tccgtataac
    18001 gtgacgaggt cttcagaggt aacatcctgc gtcaccagat tgatctgcgc gttatccgtc
    18061 gtgattctgc gaagttgaga gtctggcaca agtgcgttga cttcaacgtg tccatataaa
    18121 tagagcatcc ggtcattggt cagcttggct ttatctgctt ttacggacca tgtcgggatt
    18181 ttatccttat caaacgtggt aagtaccggc tgcgtaaacc acgaaacggc ctgatcggaa
    18241 taatattcaa cgtgttgagc aatcaatcga tagcttagtg ccccttctgg gttatagacg
    18301 agcgtgtccg tatgctcgct tttataggtg ggatcattgt tgttgacgac cacctgggcg
    18361 gtatcgtctt tttcggccat attaatgccg atcatcacca gaaccgccag tgatagcaca
    18421 atgataaccc aacgtctggc tttactcata tcgattgccc tttggcttca tccagtttgc
    18481 cctgcgccag gagtaataag tcgcaaactt cgcgcactgc gccacgaccg ccagcaatgc
    18541 gcgtcacgta atcggcgcgc gggatcaaca gtggatgcgc atcggccacg gcgacgctta
    18601 aacccacttt ttccattacc ggccagtcga tgagatcatc gccaacataa gccacatttt
    18661 ccggggcaat cgccaatttt tccagcaaat cgctaaaggc gatcagtttg tttgactgcc
    18721 cctgatacaa gtgagtgatc cccaatgtgg cacaacgatc ttctaccagt ttagcctttc
    18781 gcccggtaat gatagcgact tcaatatcag aggtgagcgc acaacgaatg ccataaccgt
    18841 cacgaacatt gaacgctttc agctcttcgc cgttattgcc catataaatc aggccatctg
    18901 acagtacgcc atcaacgtcg aggatcagca gacgaatgtt ctctgctttt gccataacgt
    18961 cggcgctgac agggccgtaa caggtcgcaa gcgacgcacc tgctttgctc attgttgttt
    19021 atccttgaat ctttacacta cgcctgcacg cagtaaatca tgcatatgta acacaccgag
    19081 taaatggtcg ccatcggcaa ccatcacgga ggtgatatgg cgggactgca ttaagttcag
    19141 tgcctcaacg gccagaatgc cagggcgcac acgtattccc cccggcgtca tcacatcggc
    19201 aatacttaac tgacgaacat ccacgcccat atcgaagaca cggcgtaaat caccgtcggt
    19261 aaagatgcct tcaatcatca tattgtcatc gcaaatgaca gtcataccaa gatttttgcg
    19321 ggtaacttcc agcaatgcgt cacgcagact ggccgttttc ttaacatgcg ggatctcatc
    19381 gcccgtatgc ataatatcgt ttacgcgcag cagaagttta cgacccagtg cgccgcctgg
    19441 gtgtgagagc gcaaaatctt cagcagtaaa gccgcgtgct tttaacagcg cgacagcgag
    19501 ggcatcgccc ataaccagcg tggcggtggt gctgctggtc ggtgccagcc ctaacggaca
    19561 ggcttctttc gctactttaa cacacagatg cacatctgcg gcgcgcgcca tgctgctctc
    19621 cgggcgaccg gtgatgcaga ttaacggtac gtgaagacgc ttaagcactg gaattaaggc
    19681 cgtgatttcg ctggattcac ctgagttaga gatagcaatc accacatcct gtggggtgac
    19741 catgcctaaa tcaccatgcg cggcttcact aggatggacg aaaaatgaag gtgtaccggt
    19801 gctggcaaac gttgcggcca ttttgcgccc gatgtgcccc gatttcccca tccccatgac
    19861 gacaactttc cctttacacc agaacatctt ttcacaggca agcgtgaaat tctgattgat
    19921 gtattgatca agctccgcca ggcattcacg ttcaatcgcc aggacttctt tacctgcttg
    19981 ctgaaagtca aaacccggtt gtaactctac gtgcgacata atgcgtttcc agttattcaa
    20041 cgagtattgg cgataaccag tacaacatcg ccagccatac gataaatccg ccagttaata
    20101 atacccctac accacggccc ggttgcgggg agcgccgcca gcacagcaac gcaaaaataa
    20161 tgctcaccag caacatcacg ctgtagtcac gactgtacgc cagtggatca atctctcctg
    20221 gcgttatcag cgcgggtaaa cccaacacga tgacaatatt aaaaatgttt gcgccaatga
    20281 tatttccgac agcaatgtcg ttttcacctt tgcgaacccc cgctattgcg gttgccagtt
    20341 ccggcagact ggttccgata gcaattgccg tcagagccat cgtcaactcg ctgatggcaa
    20401 agtaattcgc cagcaccgtg gcgttatcaa ccaccatccg cgtggccact ggcatgatga
    20461 taagcgcaat gccgagccat aaaaacgcga cgggcaatcc gccgtcacgc ggcagctctg
    20521 caagctgctc tctggtcagg ctgtcagtcc cctgacgtgc aagtttaaca atgaacagca
    20581 gccatagcac agccagaaag aggagaaaga taccatcgct tcgactaagt tgtccgtcat
    20641 agagtacgga accggccacc acgctgacca acaacattaa gggtaattca cggcgcagaa
    20701 catcggaatg gacggtaaaa ggacgaacca gcgcagccag accgaggatc agcaatatat
    20761 tgataatgtt tgagccgagg gctgtaccaa cggctaaatc gcgttgttcg tgcagagacg
    20821 cggcaagcga gacgatgatt tctggtaacg atgtaccaat actgaccacc gtcatgccga
    20881 tgatcagcgg cgggatgcca aaggttcggc aaagaataga cgcggcaaaa accaggcggt
    20941 cggcactgta aacgaccaaa agtaaaccaa caattaacag tgccgtagct aaaagcatct
    21001 aacgtccttt cttcaggtat actcgccggt ccgctgaaga ttttcagaaa gccgtaacgg
    21061 attcttaatt ttgactttat gcggctaaaa agtaaaacaa atgccagctt tcgctaacca
    21121 cggcgggtaa tattctgtaa atatgttggg ttcaaggtta aattgagcgc catgcttaga
    21181 aaatcaacgc aagacgaagg gtgaattatg gagcagtctg tggcgaattt agtcgatatg
    21241 cgcgatgtca gttttacgcg tggcaatcgc tgcatcttcg ataatatttc cctgaccgtg
    21301 ccgcgaggga agatcacggc gatcatgggg ccatcgggca tcggtaaaac gacgctactc
    21361 cgtctgattg gcgggcaaat cgcaccagat catggtgaga tccttttcga tggtgagaat
    21421 attccggcga tgtctcgttc gcgcctgtat acagtgcgta aacggatgag catgttattt
    21481 cagtccgggg cgttgttcac tgatatgaac gtatttgaca acgtcgccta tccactgcgc
    21541 gaacataccc aacttcccgc gccattgttg catagtacgg tgatgatgaa gctggaggcc
    21601 gtggggctgc gtggagcggc taaactaatg ccttctgaac tttccggtgg gatggcgcgg
    21661 cgtgcagcgc tggcgcgtgc gattgcgctg gagccggatc tcatcatgtt tgatgaacct
    21721 tttgttgggc aagatcccat caccatgggc gtactggtga agctgatttc tgagctgaac
    21781 agcgcgctgg gcgtgacttg tgtggtggtt tctcacgatg tgccggaagt gttaagtatt
    21841 gcggatcacg cctggatcct ggcggacaaa aaaattgtcg ctcatggcag tgcccaggcg
    21901 ttgcagacga atcctgatcc gcgcgtacgt cagtttctgg acgggatagc tgacgggcct
    21961 gttccgttcc gctatcctgc cggcgattat cacgctgatc ttttaccagg gagttaagcc
    22021 actcatgctg ttaaatgcgc tggcgtcgct cggacataaa gggattaaaa ccctgagaac
    22081 gttcgggcgg gccgggttaa tgttattcaa tgcgctggtc ggcaaaccgg aatttcgcaa
    22141 acatgcgccg ctgctggtgc gccagctcta taatgtcggc gtcctgtcga tgctgattat
    22201 tgtagtttct ggcgtgttca tcggaatggt gttggggctg caaggttatc tggttctgac
    22261 cacttatagc gcggaaacca gtctgggtat gctggtggcg ttatcgctac tgcgtgaact
    22321 ggggccggtg gttgccgcgc tgttgtttgc cgggcgtgct ggttcggcgt taaccgcaga
    22381 aatcggcctg atgcgcgcta cagagcaact ctccagtatg gagatgatgg cggtggatcc
    22441 gctgcgtcgg gttatttctc cccgtttctg ggctggggtt atttcattac cactgttgac
    22501 ggttatcttc gtcgccgtgg ggatctgggg cggatcgctg gtcggcgtca gttggaaagg
    22561 cattgatagc gggttcttct ggtcggcaat gcaaaatgcc gtcgactggc gtatggatct
    22621 ggtcaactgt ctgattaaga gcgtggtgtt cgccatcacg gtgacgtgga tttcgttgtt
    22681 taacggctac gacgccatcc cgacgtctgc cgggattagc cgggcaacca ctcgcaccgt
    22741 tgtccactcg tctctggctg ttctggggct ggattttgtg ctgaccgcat tgatgtttgg
    22801 gaattgagtt catgcaaacg aaaaaaaatg aaatttgggt gggtatcttt ttattagcag
    22861 cactgctggc ggcgctgttt gtttgcctga aggcggcgaa cgtgacgtcc atacgtactg
    22921 aatcgaccta cacgctttat gcgacgttcg ataacattgg cggcctgaaa gcgcgctctc
    22981 cggtcagcat tggtggtgtt gttgtgggcc gggtggcgga tattacgctg gacccgaaaa
    23041 cctatctgcc gcgcgtaacg ctggaaattg aacaacgtta taaccacatt cctgatacca
    23101 gttcgctgag cattcgtact tccggcctgc tgggagaaca atatctggca ttaaacgtcg
    23161 gttttgaaga cccggaactg gggactgcta tcctgaagga tggcgataca attcaggaca
    23221 ccaagtctgc gatggtgctg gaagatctca ttggtcagtt cctttacggt agtaaaggcg
    23281 atgacaataa gaatagtggc gatgcgccag ctgctgcgcc aggtaataat gaaaccactg
    23341 aacctgtggg tacaacgaaa taatttcagg agaaccgacg catgtttaaa cgtttaatga
    23401 tggtcgcttt gctggtgatt gcacctctga gtgcggcaac cgcggcagac cagaccaatc
    23461 cttataagct gatggacgag gcggcgcaga aaacgttcga tcgcctgaag aatgagcaac
    23521 cgcaaattcg ggccaacccg gattatctgc gtaccattgt tgatcaggaa ctgctgccat
    23581 acgtacaggt gaaatacgcc ggtgcgctgg tgctgggcca gtattacaag agtgcgaccc
    23641 ctgctcaacg tgaagcctac tttgccgctt tccgtgagta cctgaagcag gcttacggtc
    23701 aggcgctggc gatgtatcac ggtcaaacct atcagattgc gccagaacag ccgctgggcg
    23761 ataaaaccat tgtgcctatt cgcgttacca ttattgaccc gaatggccgt ccgccggtgc
    23821 gtctggactt ccagtggcgt aaaaactccc agacgggaaa ctggcaagct tacgacatga
    23881 ttgctgaagg cgtcagtatg atcaccacca aacaaaacga gtggggaacg ctgctgcgta
    23941 ccaaaggtat cgacggcctg actgcgcaac tgaaatcgat ttctcaacag aaaatcactc
    24001 tggaagagaa aaaataatga gcgagtcact gagctggatg cagacgggtg acacgctggc
    24061 gttatccgga gagctggatc aggacgttct gctaccgctt tgggaaatgc gtgaggaggc
    24121 ggtgaagggg attacctgca tcgatcttag ccgtgtgtcc cgcgtggata cggggggact
    24181 ggcactgctg ctccatctta tcgatctggc gaaaaagcag ggcaacaacg tgacgcttca
    24241 gggggtaaac gacaaagtgt ataccctcgc gaaattgtat aatttgcctg ctgatgttct
    24301 gcctcgttaa attttttcag gccggatatg gcgttaaccg cctgtccggc aaacctgcct
    24361 gcaagtcgag ccttgtctga tttatcagcg aggctttttg cttatttatg ccagcgcaac
    24421 tttgctctaa gatgtttcgc tggtttatta actgatgatt gaagatccca tggaaaataa
    24481 tgaaattcag agcgtgttga tgaacgctct ctccctccag gaagtccacg tttccggcga
    24541 tggcagccac tttcaggtta ttgccgtggg tgagttgttt gacggtatga gtcgggttaa
    24601 aaaacagcag acggtctatg gcccgctgat ggaatatatt gcggataacc gcattcatgc
    24661 tgtgtcgatc aaagcgtata cccctgcgga gtgggcgcgc gatcgcaaac tgaacggctt
    24721 ttgagctatg ggcgattcgc cggtagcgga tatgaattgt taactgagaa caaactaaat
    24781 ggataaattt cgtgttcagg ggccaacgaa gctccagggc gaagtcacaa tttccggcgc
    24841 taaaaatgct gctctgccta ttctttttgc cgcactactg gcggaagaac cggtagagat
    24901 ccagaacgtc ccgaaactga aagacgtcga tacatcaatg aaactgctaa gccagctggg
    24961 tgcgaaagta gaacgtaatg gttctgtgca tattgatgcc cgcgacgtta atgtattctg
    25021 cgcaccttac gatctggtta aaaccatgcg tgcttctatc tgggcgctgg ggccgctggt
    25081 agcgcgcttt ggtcaggggc aagtttcact acctggcggt tgtacgatcg gtgcgcgtcc
    25141 ggttgatcta cacatttctg gcctcgaaca attaggcgcg accatcaaac tggaagaagg
    25201 ttacgttaaa gcttccgtcg atggtcgtct gaaaggcgca catatcgtga tggataaagt
    25261 cagcgttggc gcaacggtga ccatcatgtg tgctgcaacc ctggcggaag gcaccacgat
    25321 tattgaaaac gcagcgcgtg aaccggaaat cgtcgatacc gcgaacttcc tgattacgct
    25381 gggtgcgaaa attagcggtc agggcaccga tcgtatcgtc atcgaaggtg tggaacgttt
    25441 aggcggcggt gtctatcgcg tgctgccgga tcgtatcgaa accggtactt tcctggtggc
    25501 ggcggcgatc tctcgtggca aaattatctg ccgtaacgcg cagccagata ctctggacgc
    25561 cgtgctggcg aaactgcgtg atgctggagc ggacatcgaa gtcggcgaag actggattag
    25621 cctggatatg catggcaaac gtccgaaggc tgttaacgta cgtaccgcgc cgcatccggc
    25681 attcccgacc gatatgcagg cccagttcac gctgttgaac ctggtggctg aagggaccgg
    25741 gttcatcacc gaaacggtct ttgaaaaccg ctttatgcat gtgccagagc tgagccgtat
    25801 gggcgcgcac gccgaaatcg aaagcaatac cgttatttgt catggtgttg aaaaactttc
    25861 tggtgcacag gttatggcaa ccgatctgcg tgcatcagca agcctggtgc tggctggctg
    25921 tattgcggaa gggacgacgg tggttgatcg tatttatcac atcgatcgtg gctacgaacg
    25981 cattgaagac aaactgcgcg ctttaggtgc aaatattgag cgtgtgaaag gcgagtaatc
    26041 gtctgagagc tggcagcccc gttcgcgggg ctaccgccag accactcatt ttttcggttt
    26101 agtgtagcta cgcatcaact gcgttctgtc gataaactca tgggtctgcg gatcatggta
    26161 gcgtgatggc cagataaccc aggggtcagt tcccagggct ttcgcaataa tcatctctcc
    26221 tttcggccat gggcgcgata atgcgtttgc cagcgttgag gaactcaaac catttctgcg
    26281 agattccgcc gccattgacg ttcctttctt gcgcaaacct gcaatgatgt cagcgggatg
    26341 ccagtcaatg aaattacttt ccatctttcc tcactcctta agttagtcgc tttcattcag
    26401 aacacttctt ctgtcgggca tcactatact cctgatggtc tattgcgcta aaaagttcct
    26461 gtaaagagtg tgaatattca gaatatataa agattcatcg cccttttttc aggtgttata
    26521 gcgctgaatt agtgttttta tggaatttac tggaatcatc ccgcgcactg cgcgggatga
    26581 agggagggga ttaacgatcg cgttgaacag cgatgtgcgc gaggccgatg agtgcttctc
    26641 gccaaggggt gtccgggagc acctgtaacg ctgcgatggc tttgtctgct tcttcctcgg
    26701 cgcgctgacg cgtccattca agagatccac aagcgttcat tgcttccaga accggttcca
    26761 gaagatggcg accgttacct tgttcgatgg cggtacggat catctgtgcc tgttctggtg
    26821 tgccatgatg catcgcatgc agcagcggca gcgtcggttt accttcgttc agatcgtcgc
    26881 cgacgttttt acctaactgt tcgccatcgg cattgtaatc gagtaaatcg tcgatcaact
    26941 ggaaagcagt gccgagatag cgcccataat cctgcaggcc tttctcctcc tccggcgtac
    27001 agccagccag aatcccggaa cactgcgcgg cagcctcaaa cagacgcgcg gttttgctat
    27061 agataacgcg catgtagttt tcttcagtga tgtccggatc gttaacgttc atcagttgca
    27121 gaacttcacc ttctgcgatg acgtttacag cttctgacat gacttccagc actttgagtg
    27181 aaccgaggct ggtcatcatc tggaacgcgc gggtataaat aaaatcgcct accagcacgc
    27241 tggcggcatt gccaaatgcg gcgttggcgg tagctttacc cctgcgcata tctgattcat
    27301 ccacaacgtc gtcgtgtagc agagtcgccg tgtggataaa ctcgatcagg gcagcaatgg
    27361 tgacatgcgc atttccctca tagccaacag ctcgtgcagc cagtacagca atcatcggac
    27421 gaatacgttt accgccgccg ctgacgatgt aatagcctaa ctgattgatc agttggacgt
    27481 cggaattaag ctgctcaagg attgccgcat taacacccgc catatcttgc gcggttaact
    27541 cattgatttt ttctaaattc atcgcaaaag ccgggctttt cgccccgatt accttactgt
    27601 gaacaaaggc aacttagggt ttatggttag gaatataggg taattgtact gaaaaaatgg
    27661 cacagataaa cgttaccgta caagttgtgt ttttttcttc gtgtattgac tgtagcactt
    27721 gtcaaaggcg tgcgttttgc gtaatattcg cgccctattg tgaatattta tagcgcactc
    27781 tgaatcattg aaaaggtgtg cgcggaagcg gagttttata tgtacgcggt tttccaaagt
    27841 ggtggtaaac aacaccgagt aagcgaaggt cagaccgttc gcctggaaaa gctggacatc
    27901 gcaactggcg aaactgttga gttcgctgaa gtgctgatga tcgcaaacgg tgaagaagtc
    27961 aaaatcggcg ttcctttcgt tgatggcggc gtaatcaaag ctgaagttgt tgctcacggt
    28021 cgtggcgaga aagttaaaat cgttaagttt cgtcgtcgta aacactatcg taagcagcag
    28081 ggccatcgtc agtggttcac tgatgtgaaa attactggca tcagcgccta agacctgagg
    28141 agagatttca aatggcacat aaaaaggctg gcggctccac acgtaacggt cgcgattcag
    28201 aagctaaacg cttgggcgtt aagcgtttcg gtggcgaatc cgttctggcg ggtagcatca
    28261 tcgttcgtca acgtggtacc aaattccacg ctggcgctaa cgtaggttgc ggtcgtgacc
    28321 acactctgtt tgctaaagca gacggtaaag tgaaattcga agttaaaggc ccgaaaaacc
    28381 gtaaatttat cagcatcgaa gctgaataag tttttcgcgt cccggtaacg gatgaaagcc
    28441 ccgcaacacg ttgcggggct ttttacattc gataaccggt aaaaatgcgc atggtgcgac
    28501 gttaatccgc aaatgcgctt tgcagggaat acggtatgaa gcagcaggca ggcattggca
    28561 ttcttttggc gcttaccaca gcaatttgct ggggggcgtt gccaatcgca atgaagcagg
    28621 tgctggaggt gatggaacct ccgacaatcg tgttttaccg tttcttgatg gcgagtattg
    28681 gccttggtgc cattcttgcg gtgaagaaga ggttgccgcc attacgcgtg tttcgtaagc
    28741 cacgctggtt gattttgttg gcagtggcga ccgccgggct gtttgggaac ttcatcctgt
    28801 tcagctcatc cttgcaatac ctgagtccga ccgcttcgca ggtgattggg caactctcgc
    28861 cagttggcat gatggttgcc agcgtattta tcctgaaaga gaaaatgcgc agcactcagg
    28921 ttgtaggggc attgatgctc ctgagcggtc tggtgatgtt ttttaacacc agtctggtcg
    28981 agatatttac aaagctcacc gattacacct ggggagttat ctttggggtc ggtgcggcga
    29041 cggtttgggt gagttatggc gtggcgcaaa aggttttatt gcgtcggctg gcctcaccgc
    29101 agatcctgtt tttactgtac actttatgta caatcgcgct cttccctctg gcaaagcctg
    29161 gagtgatagc gcagcttagc cactggcagc tcgcatgttt aattttttgc ggactgaata
    29221 ccttggtagg atatggcgcc ttggcggaag cgatggctcg ctggcaggca gcgcaggtga
    29281 gcgcgatcat cacgctcacc ccactgttta cgctgttttt ttcagatctt ttatcactgg
    29341 cctggcccga tttcttcgcc agaccgatgt taaacctttt aggttatctc ggtgcgtttg
    29401 tcgtggttgc gggcgcgatg tattccgcca ttggtcatcg tatttggggc ggattacgta
    29461 agcatacaac ggtggtatcg caaccccgcg caggcgaatg atttacggag aataaaatga
    29521 agtttgttga tgaagcatcg attctggtcg ttgcaggtga tggcggtaat ggttgcgtga
    29581 gcttccgccg cgaaaagtat attccgaaag gcggcccgga tggcggcgac ggcggtgatg
    29641 gtggtgacgt atggatggaa gccgacgaga acctgaacac gcttatcgat tatcgttttg
    29701 aaaaatcttt ccgtgcagag cgcggtcaga acggcgcaag ccgcgattgt accggtaagc
    29761 gcggtaaaga cgtgacgatt aaagtgccgg taggtacgcg tgtaatcgac cagggtactg
    29821 gtgaaaccat gggtgatatg actaaacacg gtcagcgtct gctggttgct aagggcggct
    29881 ggcacggtct gggcaatacc cgtttcaaat cgtcagttaa ccgtacacca cggcagaaaa
    29941 ctaacggtac gccgggcgat aagcgtgagc tgctgctgga gctgatgctg ctggctgacg
    30001 tcggtatgtt ggggatgcca aacgcgggta aatcgacctt tattcgtgcg gtatcggcgg
    30061 ctaaaccgaa agtggcggat tatccgttta ccactctggt gccaagtctg ggtgtggtac
    30121 gaatggacaa cgaaaagagc ttcgttgttg ccgatattcc aggactgatt gaaggcgctg
    30181 cggaaggcgc aggtctgggc attcgcttcc tgaagcacct ggaacgttgc cgcgtcctgt
    30241 tgcacctcat cgatatcgat ccgattgacg gcaccgatcc ggttgaaaac gcgcgtatta
    30301 ttatcagcga gctggaaaaa tacagccagg atctggcggc gaaaccgcgt tggttagtct
    30361 tcaacaagat cgatctgctg gataaggcag aagccgaaga gaaagcgaaa gcgaaagcga
    30421 tcgctgaagc gctgggctgg gaagataaat attatctgat ctctgcggcg agcggactgg
    30481 gcgtgaaaga tctctgctgg gatgtgatga cctttatcat tgaaaacccg gtcgtgcagg
    30541 ctgaagaagc gaaacagcca gagaaagtcg aattcatgtg ggatgattat caccgtcagc
    30601 agcttgaaga gattgctgaa gaggatgatg aagactggga tgacgactgg gacgaagacg
    30661 acgaagaagg cgttgagttc atttacaagc gttaatcact aatgaccaat gccaaataag
    30721 cctgatgcgc tacacttatc aggcctacaa gatgattgca atatattgaa tttgcacgat
    30781 tttgtaggcc ggataaggcg ttcacgccgc atccggcata aacaaagcgc actttgtcaa
    30841 taatctgaaa ccccggccat gcgccggggt ttctttttga ctaattgttc tgataaatat
    30901 ctttgtacaa acggctttca aaacgcacta acggaatacg gcgattacgc tgatccgcgg
    30961 gttttactgc atagccagaa agatattgta caaatgccat tcgttgcccg ctcgctgtgg
    31021 taatgaatcc cgccaggtta tataccccct gcaacgaacc ggttttcgct gagactttgc
    31081 catccacgcc cgcctgatgc agacctgcac ggtactgcaa agagccgtca tagcccgcaa
    31141 gcggcagcat ggagataaag ttaagttcat tgtcgtgttg ggcaatgtat tgcagcacct
    31201 gcatcatggt ggcgggggca atcaggttat gccgcgaaag ccctgaacca tcggcaataa
    31261 tggtgttacc aatatcgaca ccggcttgct ggcgcaggat ctgacgcacg gcgtccgacc
    31321 cagcccgcca tgttccaggc acattgaagc gcgcatggcc gatcatgcgg aaaaccgtat
    31381 cggcgatcat gttgtccgac tttttcagca taatcttaag cagatcgtgc agcggggccg
    31441 actgtttact ggcaactacc gttccaggtt cgttaacctg agtctggcgc agcagtgttc
    31501 cgctccaggt gatacccgcc tgttttaact catctttcag aattgcaccg gcatagctgg
    31561 ctccatcctg cacggcaaaa gccaacggga gcggctcaga acgttgtggc aggcatcccg
    31621 tcagcgtaaa gcggttcagg tcgcttggca ccacatccag ttcgcagtat tgcgcttcgg
    31681 cagaaccacg ggggagggtg cgtacctggc tgaacatcgt aacggggtaa taagatgcca
    31741 cgcgtataaa agccatatca ccaggctttg gggcactgta gagcgagacg gagaaacagt
    31801 tgcggtcaac tatggcggca gcaggcggag cgctaaagca ttgtgtcatg tcattccatg
    31861 gccagccggg ggctttatcg tggctggcga aaatggaggt atctatcaac acattgccat
    31921 cgatttggtt gacgccagat tttttcaaag tcgcgaccat attgcgaata tcctgacgtt
    31981 ttaacgtcgg atcggcacca aatcgcgcca ctaagtcacc cttaagtacg ccgttttcca
    32041 cattgccttt ggtttcaagc gtcgtggtga aacgaaaatc ggggccgagt tgaatcaacg
    32101 ccgccagcgc agtaatcact ttctgggtac tggcgggcag cgccatctgc tgactgtggt
    32161 aatcaatagc gggggccgac gcaccgactt tttgcaccat cagggcaagg ttggcacctg
    32221 cggggagttg agtaacgtac tcatcaacat tcgcggcctg aacactgaac gctatacagc
    32281 tggtcaatcc gatgataaat ctggaaaatc gcataatctc gcgctaacaa cctggaatcg
    32341 agccgtcata ctacggcgca acgccctata aagtaaacga tgacccttcg ggaacttcag
    32401 ggtaaaatga ccatcaaaat gtgaattgta gctgacctgg gacttgtacc cgggtcggta
    32461 tttttttgct tctggtcccg gtgaggagtt atgccgggca ggccgaacag ccggggtggg
    32521 tgaagacttg ccccatcagg aatattcaag aggtataaca aatgcaagct attccgatga
    32581 ccttacgcgg cgctgaaaaa ttacgcgaag agctggattt tctgaaatct gtgcgccgtc
    32641 ctgaaatcat tgctgctatc gcggaagcgc gtgagcatgg cgacctgaaa gaaaacgccg
    32701 aataccacgc agctcgtgaa cagcagggct tctgcgaagg ccgtatcaaa gacatcgaag
    32761 ccaagctgtc aaacgcgcag gtgattgatg tcaccaaaat gcccaacaat gggcgcgtta
    32821 tttttggtgc taccgtaacg gtgctgaatc tggattctga cgaagaacag acttatcgca
    32881 tcgttggcga tgacgaagct gactttaaac aaaacctgat ttctgtaaac tcgcctattg
    32941 ctcgtggcct gatcggcaaa gaagaagatg atgttgtggt catcaaaacg ccgggcggcg
    33001 aagtagaatt tgaagtaatt aaggtggaat acctgtaaga attacccaat actcaagatg
    33061 ttgatgtatt gtaaagaaag gaaaaaggcc gctctgcggc cttttatcaa cgaacagagc
    33121 gtggcatttt gctctcctgc ctgcggaaaa cccctcgttt tacacagcaa atgtgtgtaa
    33181 ctttaggata atcttagcgt ggcagcgaga ttttacgttc tttagttggg cgataaagca
    33241 ccagcgtttt accgatgacc tgtacattac aggcgccggt ttcgcgcacg atagcttcca
    33301 cgatcaaggt ttttgtttcg cgatcttcgg tggcgatttt caccttgatg agttcatggt
    33361 gctctaacgc ttgttcaatc tcggccagca ccccttcggt caaaccatta ctgccaagca
    33421 gaacaactgg cttgagcgga tgtgccagac ctttcaggtg ctgtttttgt ttagtactca
    33481 gattcatcgt attttttgct tacgttggga ttgaaaacgg gtcattctac cgccatctcc
    33541 catatatcac caaataggcg cgtaaaaatt tacgcaattg gttacgatga gttatcccca
    33601 tgggaaagtt aaatgacagg taagaagcgt tctgccagct ccagccgctg gcttcaggaa
    33661 cactttagcg ataaatatgt tcaacaggca cagaaaaagg gattacgttc ccgtgcctgg
    33721 tttaaacttg atgaaataca gcaaagtgac aaactcttta aaccgggaat gacggttgtc
    33781 gaccttggtg ctgctccggg tggttggtca caatatgtgg tcacccaaat tggcggcaaa
    33841 ggccgcatca tcgcttgcga tcttttacct atggatccta tcgttggtgt ggactttctt
    33901 cagggcgatt ttcgtgatga actggtgatg aaagcactgc tggagcgcgt tggcgacagc
    33961 aaagtccagg ttgtcatgtc cgatatggca ccaaacatga gcggaacacc ggcggtggat
    34021 atcccccgag ccatgtatct ggtggaactg gcgctagaaa tgtgtcgtga tgtattagcg
    34081 ccaggtggca gttttgtagt gaaggtgttc cagggcgaag gtttcgatga gtatctaagg
    34141 gaaattcgct ccctgtttac gaaggtcaaa gttcgtaagc cggactcttc tcgtgcacgt
    34201 tcgcgagaag tgtatattgt agcgaccggg cgtaaaccct aaccgggaga tttcagacga
    34261 aagtttgaaa gatgctggat atagagtatc ctgacgctgt ttttaacaca gttgtaataa
    34321 gaggttaatc ccttgagtga catggcgaaa aacctaatac tctggctggt cattgccgtt
    34381 gtgctgatgt cagtattcca gagctttggg cccagcgagt ctaatggccg taaggtggat
    34441 tactctacct tcctacaaga ggtcaataac gaccaggttc gtgaagcgcg tatcaacgga
    34501 cgtgaaatca acgttaccaa gaaagatagt aaccgttata ccacttacat tccggttcag
    34561 gatccgaaat tactggataa cctgttgacc aagaacgtca aggttgtcgg tgaaccgcct
    34621 gaagaaccaa gcctgctggc ttctatcttc atctcctggt tcccgatgct gttgctgatt
    34681 ggtgtctgga tcttcttcat gcgtcaaatg cagggcggcg gtggcaaagg tgccatgtcg
    34741 tttggtaaga gcaaagcgcg catgctgacg gaagatcaga tcaaaacgac ctttgctgac
    34801 gttgcgggct gcgacgaagc aaaagaagaa gttgctgaac tggtagagta tctgcgcgag
    34861 ccgagccgct tccagaaact cggcggtaag atcccgaaag gcgtcctgat ggtcggtcct
    34921 ccgggtaccg gtaaaacgtt gctggcgaaa gcgattgcag gcgaagcgaa agttccgttc
    34981 tttactatct ccggttctga cttcgtagaa atgttcgtcg gtgtgggtgc atcccgtgtt
    35041 cgtgacatgt tcgaacaggc gaagaaagcg gcaccgtgca tcatctttat cgatgaaatc
    35101 gacgccgtag gccgccagcg tggcgctggt ctgggcggtg gtcacgatga acgtgaacag
    35161 actctgaacc agatgctggt tgagatggat ggcttcgaag gtaacgaagg tatcatcgtt
    35221 atcgccgcga ctaaccgtcc ggacgttctc gacccggccc tgctgcgtcc tggccgtttc
    35281 gaccgccagg ttgtggtcgg cttgccagat gttcgcggtc gtgagcagat cctgaaagtt
    35341 cacatgcgtc gcgtaccatt ggcacccgat atcgacgcgg caatcattgc tcgtggtact
    35401 cctggtttct ccggtgctga cctggcgaac ctggtgaacg aagcggcact gttcgctgct
    35461 cgtggcaaca aacgcgttgt gtcgatggtt gagttcgaga aagcgaaaga caaaatcatg
    35521 atgggtgcgg aacgtcgctc catggtgatg acggaagcgc agaaagaatc aacggcttac
    35581 cacgaagcgg gtcatgcgat tatcggtcgc ctggtgccgg aacacgatcc ggtgcataaa
    35641 gtgacgatta tcccacgcgg tcgtgcgctg ggtgtgactt tcttcttgcc tgagggcgac
    35701 gcaatcagcg ccagccgtca gaaactggaa agccagattt ctacgctgta cggtggtcgt
    35761 ctggcagaag agatcatcta cgggccggaa catgtttcta ccggtgcgtc caacgatatt
    35821 aaagttgcga ccaacctggc acgtaacatg gtgactcagt ggggcttctc tgagaaattg
    35881 ggtccactgc tgtacgcgga agaagaaggt gaagtattcc tcggccgtag cgtagcgaaa
    35941 gcgaaacata tgtccgatga aactgcacgt atcatcgacc aggaagtgaa agcactgatt
    36001 gagcgtaact ataatcgtgc gcgtcagctt ctgaccgaca atatggatat tctgcatgcg
    36061 atgaaagatg ctctcatgaa atatgagact atcgacgcac cgcagattga tgacctgatg
    36121 gcacgtcgcg atgtacgtcc gccagcgggc tgggaagaac caggcgcttc taacaattct
    36181 ggcgacaatg gtagtccaaa ggctcctcgt ccggttgatg aaccgcgtac gccgaacccg
    36241 ggtaacacca tgtcagagca gttaggcgac aagtaagttc ccgcatcaga tgactgtatt
    36301 tgtaccgaaa accccggggc gtgctccggg gttttttctt atcaattcat accagggata
    36361 acataatgaa actctttgcc cagggtactt cactggacct tagccatcct cacgtaatgg
    36421 ggatcctcaa cgtcacgcct gattcctttt cggatggtgg cacgcataac tcgctgatag
    36481 atgcggtgaa acatgcgaat ctgatgatca atgctggcgc gacgatcatt gacgttggtg
    36541 gcgagtccac gcgcccaggg gcggcggaag ttagcgttga agaagagttg caacgtgtta
    36601 ttcctgtggt tgaggcaatt gctcaacgct tcgaagtctg gatctcggtc gatacatcca
    36661 aaccagaagt catccgtgag tcagcgaaag ttggcgctca cattattaat gatatccgct
    36721 ccctttccga acctggcgct ctggaggcgg ctgcagaaac cggtttaccg gtttgtctga
    36781 tgcatatgca gggaaatcca aaaaccatgc aggaagctcc gaagtatgac gatgtctttg
    36841 cagaagtgaa tcgctacttt attgagcaaa tagcacgttg cgagcaggcg ggtatcgcaa
    36901 aagagaaatt gttgctcgac cccggattcg gtttcggtaa aaatctctcc cataactatt
    36961 cattactggc gcgcctggct gaatttcacc atttcaacct gccgctgttg gtgggtatgt
    37021 cacgaaaatc gatgattggg cagctgctga acgtggggcc gtccgagcgc ctgagcggta
    37081 gtctggcctg tgcggtcatt gccgcaatgc aaggcgcgca catcattcgt gttcatgacg
    37141 tcaaagaaac cgtagaagcg atgcgggtgg tggaagccac tctgtctgca aaggaaaaca
    37201 aacgctatga gtaatcgtaa atatttcggt accgatggga ttcgtggtcg tgtaggggat
    37261 gcgccgatca cacctgattt tgtgcttaag ctgggttggg ccgcgggtaa agtgctggcg
    37321 cgccacggct cccgtaagat tattattggt aaagacacgc gtatttctgg ctatatgctg
    37381 gagtcagcac tggaagcggg tctggcggca gcgggccttt ccgcactctt cactggcccg
    37441 atgccaacac cggccgtggc ttatctgacg cgtaccttcc gcgcagaggc cggaattgtg
    37501 atatctgcat cgcataaccc gttctacgat aatgggatta aattcttctc tatcgacggc
    37561 actaaactgc cggatgcggt agaagaggcc atcgaagcgg aaatggaaaa ggagatcagc
    37621 tgcgttgatt cggctgaact gggtaaagcc agccgtatcg ttgatgccgc gggtcgctat
    37681 atcgagtttt gcaaagccac gttcccgaac gaacttagcc tcagtgaact gaagattgtg
    37741 gtggattgtg caaacggtgc gacttatcac atcgcgccga acgtgctgcg cgaactgggg
    37801 gcgaacgtta tcgctatcgg ttgtgagcca aacggtgtaa acatcaatgc cgaagtgggg
    37861 gctaccgacg ttcgcgcgct ccaggctcgt gtgctggctg aaaaagcgga tctcggtatt
    37921 gccttcgacg gcgacggcga tcgcgtgatt atggttgacc atgaaggcaa taaagtcgat
    37981 ggcgatcaga tcatgtatat catcgcgcgt gaaggtcttc gtcagggcca gctgcgtggt
    38041 ggtgctgtgg gtacattgat gagcaacatg gggcttgaac tggcgctgaa acagttagga
    38101 attccatttg cgcgcgcgaa agtgggtgac cgctacgtac tggaaaaaat gcaggagaaa
    38161 ggctggcgta tcggtgcaga gaattccggt catgtgatcc tgctggataa aaccactacc
    38221 ggtgacggca tcgttgctgg cttgcaggtg ctggcggcga tggcacgtaa ccatatgagc
    38281 ctgcacgacc tttgcagcgg catgaaaatg ttcccgcaga ttctggttaa cgtacgttac
    38341 accgcaggta gcggcgatcc gcttgagcat gagtcagtta aagccgtgac cgcagaggtt
    38401 gaagctgcgc tgggcaaccg tggacgcgtg ttgctgcgta aatccggcac cgaaccgtta
    38461 attcgcgtga tggtggaagg cgaagacgaa gcgcaggtga ctgaatttgc acaccgcatc
    38521 gccgatgcag taaaagccgt ttaaagcgtt agataactgg ctaaaaaggc ggcgattgtc
    38581 gccttttttc tcggcgaaca catcggtttt gctgtttttt tccgcagttg atacaatgcg
    38641 ataaaattgc ccttgcgaag gtcattcgtt ttggttagta ttcacacccg cttcagtggg
    38701 aaagattaaa aactcccgct ttattggttg aagcattggt acgcggcaac tccgcaagga
    38761 acaggttgat tatgtacgaa gctcttttag tagttttcct tattgtggca attggccttg
    38821 ttggtctgat catgctgcag caaggtaaag gcgctgatat gggagcctcc ttcggagcag
    38881 gcgcttccgc tacgctgttt ggttcaagtg gttctggtaa cttcatgacc cgcatgacgg
    38941 cgctgctggc aacgttattc ttcatcatca gtctggtgct gggtaacatc aatagcaaca
    39001 aaaccaatag aggtagcgaa tgggaaaatc tgagtgcacc ggcgaaaacc gaacaaactc
    39061 agccagctgc tccggctaag ccgaccagcg atatcccgaa ctaaaaagta gtatccgtgc
    39121 cgaggtggtg gaattggtag acacgctacc ttgaggtggt agtgcccaat agggcttacg
    39181 ggttcaagtc ccgtcctcgg taccaaattc cagaaaagag acgctgaaaa gcgtctcttt
    39241 tcgttttggt cctggtctga tgaatggttg tcaggtatct gtatctcgtc cagttgcttt
    39301 actcgtagct ggtaccttga aacgatggtg ccggtacgcc ttagttataa acttttccct
    39361 actgctgaca taattggcac tttattgtgt caactccagt tccggtacga atacggatat
    39421 gtttacttct cgataaacca tcctgctcct gtcagtataa ctactgatgg ctacgttctg
    39481 cgtaatgttt agagacgcat tgataatgtt atttaattac tgagaacgca atggtcgcat
    39541 catttaaaaa gaaaaaacta acagtgtttc ggtctgatat ggatcagagt tgaaaaacat
    39601 gtctgatata aaattgaaca ttgttgataa ttgtttatat tggttgtgaa cttggatttc
    39661 tggatgacag tattcaacaa atttgctaga agattttaaa tctcattggt tgttgtatct
    39721 ttgtgttatt gtttttggta ttacgaactt agtcgcctct tccggagcgc atatggttca
    39781 gcgattgctg ttcttcgtcc tgaccatcct ggttgttaaa cgtatatcat cccttccgct
    39841 tcgcctgctt gttgccgcac catttgtgtt actgactgcg gcagacatga gtattagcct
    39901 ctattcatgg tgtacctttg gtacaacttt caatgatgga tttgcgatta gtgtgctgca
    39961 gagtgatccg gatgaagttg tgaaaatgct ggggatgtat atcccttatc tatgtgcttt
    40021 tgctttttta tcccttcttt ttttggcagt aataataaaa tatgatgttt ccttgccgac
    40081 aaaaaaagtg acaggaatat tattgctgat tgtcatttcg ggcagtttat tttccgcttg
    40141 tcaatttgct tataaagatg caaaaaataa aaaagcgttc agtccatata tactagcgtc
    40201 gtgatttgct acctatacgc cgtttttcaa tctcaactat tttgctttag cagcgaaagc
    40261 gcatcaaaga ttactctcaa ttgcaaacac ggtgccgtat tttcaattat cagtcaggga
    40321 tacaagtatt gatacctacg tgttgattgt gggggagtct gtacgtgtcg acaatatgtc
    40381 tttgtatgga tatacacgct ctacgacacc gcaagttgaa gcacaaagaa aacagatcaa
    40441 actgtttaat caagcaataa gcggcgcacc ttacactgcg ctgtcggttc ccctttcttt
    40501 aactgctgat tctgttttga gtcatgacat tcataattac cccgacaaca ttattaatat
    40561 ggctaatcaa gcaggatttc agactttctg gctaagctcg caatccgctt ttcggcagaa
    40621 tggtacagca gttaccagta tcgccatgcg cgccatggaa acagtttatg tcagaggatt
    40681 tgatgaattg ttgttgccgc atttatcgca agcattacag caaaatacgc agcaaaagaa
    40741 actgattgtt cttcatttaa atggaagcca tgaaccggct tgtagcgcct atccgcaatc
    40801 cagcgccgtg tttcaaccgc aggacgatca ggatgcctgc tatgacaact ccattcatta
    40861 cacagatagt ttgctaggtc aggtttttga attattaaaa gatcgccgcg cctcggtcat
    40921 gtattttgcc gaccacggcc tggaacgtga ccctacgaag aagaacgtct attttcatgg
    40981 aggcagggag gctagccagc aggcatatca tgtcccgatg tttatctggt atagccccgt
    41041 tcttggggat ggcgtggatc gcacaacgga aaacaatatc ttttcgacag cttacaataa
    41101 ttaccttatt aatgcgtgga tgggggtaac aaagccggaa cagccgcaaa cgcttgagga
    41161 agtgattgca cactataaag gagactcacg ggttgtggat gcaaaccatg atgttttcga
    41221 ttatgtgatg ctcagaaagg agtttacaga ggataagcaa ggtaacccca cccctgaagg
    41281 gcagggttga tgtcgaatta ctggcctttg ttttccatat tctccacctg cggcaggcct
    41341 gaagtggcag aggaggaaag caggccagtt ttggcataac caaaaagttt ctctcgggta
    41401 tcagtgatat ccagattacg catggtcaat tgaccaatac gatcatctgg cgagaacacc
    41461 gagtcgcctt tttccatcgt cagacgctct ggcttgtagg tcaggttctc tgagacggta
    41521 ttcaggattg aataatcgtt cccacggcgc agctccaggg taacttcacc agtgatctgg
    41581 ctggcaaccc agcgttgcag agagtcacgc agcatcagcg cctgggagtc aaaccaacgc
    41641 ccctggtaca gcagacggcc caactgacga ccatgcgcgt gatactgctc aatggtgtct
    41701 tcgttgtgaa taccggtcaa cagacgttca tacgcaatgt gcagcagtgc catccccgga
    41761 gcttcgtaaa taccacggct tttcgcttcg atgatacggt tctcaatctg gtcgctcatg
    41821 cccaggccgt gacgaccgcc gatgcggtta gcttccagca tcatttctac gtcgtcgcta
    41881 aaggttttac cgttcagcgc caccggatga ccttgctcaa agcgtactgt gacttcttct
    41941 gccgggatct tcacgctctc atcccagaat ttcacgccca taatcgggtt gacgattttg
    42001 acgctggagt tgaggtattc cagatccttc gcttcatgcg ttgcaccaag catgttggag
    42061 tctgtggagt aggctttttc gacagacatt ttgtagtcga aaccgcaggc aatcataaat
    42121 tcagacatct catgacggcc gcccagttca tcaataaagt cagtatcaag ccacggtttg
    42181 taaatctgca gttcagcatt ggtcagcaga ccataacgat agaaacgttc gatatcgttt
    42241 cctttatagg tgctaccgtc accccagata ttcacgccat cttctttcat cgcagcaacc
    42301 agcatggtac cagtcacggc gcggcccagc ggcgtcgtgt tgaaataggt caggccgccg
    42361 gtggtgttat gaaatgcgcc acactgaata gcggcaatac cttcggccac cagttgtttg
    42421 cggcagtcga tcagacgtgc gttctccgcg ccgtattcca tggcacgacg agggatcgca
    42481 tcataatcct cttcgtctgg ctggcccagg tttgcagtat atgcataagg aaccgctccc
    42541 ttttgtcgca tccacagcag tgcggcactg gtgtccagac cgccggaaaa agcgatacca
    42601 atacgttgac ctaccgggag atgcttgaga atcgtcgtca taaaataaca ccctgcttaa
    42661 ttaactgatg atgagcctgg atttccgctc tcactgaatt tttatgcaaa ataaatgagt
    42721 tttcatttaa tcatctttta tcggagacag gaagagttta gtgtgttttt tgtaaaataa
    42781 tgcgcttaag ggagagcagg ggaaggcaaa agtattcaac aaatgaaagt gaactggata
    42841 ttcattcacg tgattagcaa taaacgttga caaaatgtgg catggatcac tataatgcct
    42901 gcagatttta cgtcccgtct cggtacacca aatcccagca gtatttgcat tttttaccca
    42961 aaacgagtag aatttgccac gtttcaggcg cggggtggag cagcctggta gctcgtcggg
    43021 ctcataaccc gaaggtcgtc ggttcaaatc cggcccccgc aaccactttc ccttagagtc
    43081 ctttttcaaa tatactgtga agacttcggc cttcgtagtg ggatttgaaa aaatccttct
    43141 ggaaagtgct ccagaccgca gttgcggtta tagggttcag ttatataaag ccccgattta
    43201 tcggggtttt ttgttatctg actacagaat aactgggctt taggcccttt ttttatgtct
    43261 tgggggtggg cttgtccaca ttagagcaaa aattaacaga gatgattact gcgccagttg
    43321 aggccctggg ttttgaactg gtaggcatcg aatttattcg cggtcgcaca tccacactgc
    43381 gcatctatat tgatagtgaa gatggcatca atgttgatga ttgtgctgat gtgagccacc
    43441 aggtaagtgc tgtgctggat gttgaagatc ccatcaccgt tgcttataac ctggaagtct
    43501 cctcaccggg tctcgatcgc ccactgttca cggctgaaca ctacgcccgt tttgtcggag
    43561 aagaggtgac tctggttctc cgtatggcgg tacaaaaccg tcgtaaatgg cagggcgtta
    43621 tcaaagcggt agacggtgaa atgatcacag ttaccgtcga aggtaaagat gaagtgttcg
    43681 cgctgagtaa tatccagaag gcgaacctgg ttccccactt ttaatagtct ggatgaggtg
    43741 aaaagcccgc gatgaacaaa gaaattttgg ctgtagttga agccgtatcc aatgaaaagg
    43801 cgctacctcg cgagaagatt ttcgaagcat tggaaagcgc gctggcgaca gcaacaaaga
    43861 aaaaatatga gcaagagatc gacgtccgtg tacagatcga tcgcaaaagc ggtgattttg
    43921 acaccttccg tcgctggtta gttgttgatg aagtcaccca gccgaccaag gaaatcaccc
    43981 ttgaagccgc acgttatgaa gatgaaagcc tgaacttggg cgattacgtt gaagatcaga
    44041 ttgagtctgt tacctttgac cgtatcacca cccagacggc aaaacaggtt atcgtgcaga
    44101 aagtgcgtga agccgaacgt gcgatggtgg ttgatcagtt ccgtgaacac gaaggtgaaa
    44161 tcatcaccgg cgtggtgaaa aaagtaaacc gcgacaacat ctctctggat ctgggcaaca
    44221 acgctgaagc cgtgatcctg cgcgaagata tgctgccgcg tgaaaacttc cgccctggcg
    44281 accgcgttcg tggcgtgctt tattccgttc gcccggaagc gcgtggcgcg caactgttcg
    44341 tcactcgttc caagccggaa atgctgatcg aactgttccg tattgaagtg ccggaaatcg
    44401 gcgaagaagt gattgaaatt aaagcagcgg ctcgcgaccc gggttctcgt gcgaaaatcg
    44461 cggtgaaaac caacgataaa cgtatcgatc cggtaggtgc ttgcgtaggt atgcgtggcg
    44521 cgcgtgttca ggcggtttct actgaactgg gcggcgagcg tatcgatatc gtcctgtggg
    44581 atgataaccc ggcgcagttt gtgattaacg caatggcacc ggcagacgtt gcttctatcg
    44641 tggtggatga agataaacac accatggata tcgccgttga agctggtaac ctggcgcagg
    44701 cgattggccg taacggtcag aacgtgcgtc tggcttcgca gctgagcggt tgggaactca
    44761 acgtaatgac cgttgacgac ctgcaggcta agcatcaggc ggaagcgcac gcagcgatcg
    44821 acaccttcac caaatatctc gacatcgacg aagacttcgc gactgttctg gtagaagaag
    44881 gtttctcgac gctggaagaa ctggcctatg tgccgatgaa agagctgttg gaaatcgaag
    44941 gccttgatga gccgaccgtt gaagcactgc gcgagcgtgc taaaaatgca ctggccacca
    45001 ttgcacaggc ccaggaagaa agcctcggtg ataacaaacc ggctgacgat ctgctgaacc
    45061 ttgaaggggt agatcgtgat ttggcattca aactggccgc ccgtggcgtt tgtacgctgg
    45121 aagatctcgc cgaacagggc attgatgatc tggctgatat cgaagggttg accgacgaaa
    45181 aagccggagc actgattatg gctgcccgta atatttgctg gttcggtgac gaagcgtaat
    45241 aaactgtagc aggaaggaac agcatgacag atgtaacgat taaaacgctg gccgcagagc
    45301 gacagacctc cgtggaacgc ctggtacagc aatttgctga tgcaggtatc cggaagtctg
    45361 ctgacgactc tgtgtctgca caagagaaac agactttgat tgaccacctg aatcagaaaa
    45421 attcaggccc ggacaaattg acgctgcaac gtaaaacacg cagcaccctt aacattcctg
    45481 gtaccggtgg aaaaagcaaa tcggtacaaa tcgaagtccg caagaaacgc acctttgtga
    45541 aacgcgatcc gcaagaggct gaacgccttg cagcggaaga gcaagcccgt cgtgaggcag
    45601 aagaatcggc taaacgcgag gcgcaacaaa aagctgaacg tgaggccgca gaacaagcta
    45661 agcgtgaagc tgctgaacaa gcgaaacgtg aagctgcgga aaaagacaaa gtgagcaatc
    45721 aacaagacga tatgactaaa aacgcccagg ctgaaaaagc ccgccgtgag caggaagctg
    45781 c
//
LOCUS       DAJTSF010000023        42816 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000023 DAJTSF010000000
VERSION     DAJTSF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.023"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO335_001353"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO335_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6001806.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="OO335_001354"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="OO335_001354"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6001807.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="OO335_001355"
     CDS             complement(3736..4092)
                     /locus_tag="OO335_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6001808.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="OO335_001356"
     CDS             complement(4096..4512)
                     /locus_tag="OO335_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6001809.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO335_001357"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO335_001357"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6001810.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="OO335_001358"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="OO335_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO335_001359"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO335_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6001811.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO335_001360"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO335_001360"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6001812.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO335_001361"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO335_001361"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6001813.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="OO335_001362"
     CDS             10521..11504
                     /locus_tag="OO335_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6001814.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO335_001363"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO335_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6001815.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO335_001364"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO335_001364"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6001816.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="OO335_001365"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="OO335_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="OO335_001366"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="OO335_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6001817.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="OO335_001367"
     CDS             complement(15306..15515)
                     /locus_tag="OO335_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6001818.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO335_001368"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO335_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6001819.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO335_001369"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO335_001369"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6001820.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO335_001370"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO335_001370"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6001821.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="OO335_001371"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="OO335_001371"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6001822.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO335_001372"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO335_001372"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6001823.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO335_001373"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO335_001373"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6001824.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="OO335_001374"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="OO335_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO335_001375"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO335_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR6001825.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO335_001376"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO335_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR6001826.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO335_001377"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO335_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR6001827.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29458
                     /gene="malE"
                     /locus_tag="OO335_001378"
                     /pseudo
     CDS             28242..29458
                     /gene="malE"
                     /locus_tag="OO335_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
     gene            29612..31156
                     /gene="malF"
                     /locus_tag="OO335_001379"
     CDS             29612..31156
                     /gene="malF"
                     /locus_tag="OO335_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR6001828.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31171..32061
                     /gene="malG"
                     /locus_tag="OO335_001380"
     CDS             31171..32061
                     /gene="malG"
                     /locus_tag="OO335_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR6001829.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32432..33907
                     /gene="xylE"
                     /locus_tag="OO335_001381"
     CDS             32432..33907
                     /gene="xylE"
                     /locus_tag="OO335_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR6001830.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33951..34361)
                     /gene="psiE"
                     /locus_tag="OO335_001382"
     CDS             complement(33951..34361)
                     /gene="psiE"
                     /locus_tag="OO335_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR6001831.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34487..34630)
                     /locus_tag="OO335_001383"
     CDS             complement(34487..34630)
                     /locus_tag="OO335_001383"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001832.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34631..34909
                     /locus_tag="OO335_001384"
     CDS             34631..34909
                     /locus_tag="OO335_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001833.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34956..37052)
                     /locus_tag="OO335_001385"
     CDS             complement(34956..37052)
                     /locus_tag="OO335_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6001834.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37052..37789)
                     /locus_tag="OO335_001386"
     CDS             complement(37052..37789)
                     /locus_tag="OO335_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6001835.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37786..38424)
                     /locus_tag="OO335_001387"
     CDS             complement(37786..38424)
                     /locus_tag="OO335_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6001836.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38538..38780)
                     /locus_tag="OO335_001388"
     CDS             complement(38538..38780)
                     /locus_tag="OO335_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001837.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39243..40892)
                     /gene="pgi"
                     /locus_tag="OO335_001389"
     CDS             complement(39243..40892)
                     /gene="pgi"
                     /locus_tag="OO335_001389"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6001838.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41417..42766
                     /gene="lysC"
                     /locus_tag="OO335_001390"
     CDS             41417..42766
                     /gene="lysC"
                     /locus_tag="OO335_001390"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6001839.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT        10650 a        11019 c        10509 g        10638 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcactg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa ttatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gataaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaatagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca gggactggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 caataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagcggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgat
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg gtcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctccctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaact
    28621 tacatgggat gccgtacgtt acaacggcaa gctgattgct tacccaatcg ctgttgaagc
    28681 gttatcgctg atttataaca aagacctgct gccgaacccg ccaaaaacct gggaagagat
    28741 cccggcgctg gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca
    28801 agaaccgtac ttcacctggc cgctgattgc tgctgacggg ggttatgcgt tcaagtatga
    28861 aaacggcaag tacgacatta aagacgtggg cgtggataac gctggcgcga aagcgggtct
    28921 gaccttcctg gttgacctga ttaaaaacaa acacatgaat gcagacaccg attactccat
    28981 cgcagaagct gcctttaata aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg
    29041 gtccaacatc gacaccagca aagtgaatta tggtgtaacg gtactgccga ccttcaaggg
    29101 tcaaccatct aaaccgttcg ttggcgtgct gagcaccggt attaacgccg ccagtccgaa
    29161 caaagagctg gcgaaagagt tcctcgaaaa ctatctgctg actgatgaag gtctggaagc
    29221 ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg aagagttggc
    29281 gaaagatcca cgtattgccg ccaccatgga aaacgcccag aaaggtgaaa tcatgccgaa
    29341 catcccgcag atgtccgctt tctggtatgc cgtacgtact gcggtgatca acgccgccag
    29401 cggtcgtcag actgtcgatg aagccctgaa agacgcgcag actcgtatca ccaagtaatg
    29461 ctgtgaaatg ccggatgcgg cgtgaacgcc ttgtccggcc tacaaaactg aaacgcatgt
    29521 aggcctgata agacgcatca gtgtcgcatc aggcagttgt tgtcggataa ggcgtgaaag
    29581 ccttatccgt cctggaatga ggaagaaccc catggatgtc attaaaaaga aacattggtg
    29641 gcaaagcgac gcgctgaaat ggtcagtgct aggtctgctc ggcctgctgg tgggttacct
    29701 tgttgtttta atgtacgcac aaggggaata cctgttcgcc attaccacgc tgatattgag
    29761 ttcagcgggg ctgtatattt tcgccaatcg taaagcctac gcctggcgct atgtttaccc
    29821 gggaatggct ggaatgggat tattcgtcct cttccctctg gtctgcacca tcgccattgc
    29881 cttcaccaac tacagcagca ctaaccagct gacttttgaa cgtgcgcagg aagtgttgtt
    29941 agatcgctcc tggcaagcag gcaaaaccta taactttggt ctttacccgg cgggcgatga
    30001 gtggcaactg gcgctcagcg acggcgaaac cggcaaaaat tacctctccg acgcgtttaa
    30061 atttggcggc gagcaaaaac tgcaactgaa agaaacgacc gcccagcccg aaggcgaacg
    30121 cgcgaatctg cgcgtgatta cccagaatcg tcaggcgctg agtgacatta ccgccattct
    30181 gccggatggc aacaaagtga tgatgagctc cctgcgccag ttttctggca cgcagccgct
    30241 ctacacactc gacggtgacg gcacgttgac gaataatcag agtggcgtga aatatcgtcc
    30301 gaataaccaa attggctttt accagtccat taccgccgac ggcaactggg gggatgaaaa
    30361 gctaagcccc ggttacaccg tgaccaccgg ctggaaaaac tttacccgcg tctttaccga
    30421 cgaaggcatt cagaaaccgt tcctcgccat tttcgtctgg accgtggtgt tctcgctgat
    30481 cactgtcttt ttaacggtgg cggtcggcat ggttctggca tgtctggtgc agtgggaagc
    30541 gttgcgcggc aaagcggtct atcgcgtcct gctgattctg ccctacgcgg tgccatcgtt
    30601 catttcaatc ttgattttca aaggattgtt taaccagagc ttcggtgaaa tcaacatgat
    30661 gttgagcgcg ctgtttggcg tgaagcccgc ctggttcagc gatccgacca ccgctcgcac
    30721 gatgctgatt atcgtcaata cctggctggg ttatccgtac atgatgatcc tctgcatggg
    30781 cttgctgaaa gcgattccgg acgatttgta tgaagcctca gcaatggatg gcgcaggtcc
    30841 gttccagaac ttctttaaga ttacgctgcc gctgctgatt aaaccgctga cgccgctgat
    30901 gatcgccagc ttcgccttta actttaacaa cttcgtgctg attcagctgt taaccaacgg
    30961 cggcccggat cgtcttggca cgaccacgcc agccggttat accgacctgc ttgttaacta
    31021 cacctaccgt atcgcttttg aaggcggcgg gggtcaggac ttcggtctgg cggcagcaat
    31081 tgccacgctg atcttcctgc tggtgggtgc gctggcgata gtgaacctga aagccacgcg
    31141 aatgaagttt gattaaggga gataacaaaa atggcaatgg tccaaccgaa atcgcaaaaa
    31201 gcacgtttgt ttattactca cctgctactg ctacttttta tcgcggcgat tatgttcccg
    31261 ctgctgatgg tcgtcgctat ctcgctgcgt cagggaaact tcgcgaccgg tagcctgatc
    31321 ccggagcaaa tctcctggga tcactggaaa ctggcgttag gttttagcgt tgaacaggct
    31381 gatggtcgca ttacgccacc gccattcccg gtactgctgt ggctgtggaa ctcggtaaag
    31441 gtcgctggga tttccgcgat tggcattgtg gcgctctcca ccacctgcgc ctacgctttc
    31501 gcccgtatgc gctttccagg caaagcgacg ctgctgaaag gaatgctgat tttccagatg
    31561 ttcccggcag tactttcact ggtcgcgttg tatgcgttgt ttgatcgtct gggtgagtac
    31621 attccattca ttggcctgaa tactcacggc ggcgtaattt tcgcgtatct gggtgggatt
    31681 gcgctgcatg tctggaccat caaaggctat ttcgaaacca tcgacagttc gctggaagaa
    31741 gcggctgcgc tggatggtgc gacaccgtgg caggccttcc gccttgtcct gttgccgctg
    31801 tcagtaccga ttctggcggt ggtattcatc ctgtcgttta tcgctgccat tactgaagtt
    31861 ccggtcgcgt cgctgttact gcgtgacgta aacagctaca ccctggccgt ggggatgcag
    31921 caatacctca acccgcaaaa ctacctgtgg ggtgactttg ccgccgctgc cgtgatgtct
    31981 gcattaccga tcaccatcgt cttcttgctg gctcaacgct ggctggtcaa cggcctgacg
    32041 gcaggtggtg tgaaaggtta aagatgttgt tctgccaatg ttatgccgct gcaccctcaa
    32101 cttacgttat cccaacttgt gactattatt cggcgctcca cggagcgcct ttttttcttt
    32161 cgtctgcaat ctgaatcgtt cgccggttaa tattcccatc atagaactta ttatttttac
    32221 gttatttgtt ttcccactta cgataattct ctttcgtgct ctgagtcacg gcaatagtat
    32281 tgtttcatca atttttgata attatcacaa ttaagatcac agaaaagaca ttacgtaaac
    32341 gcattgtaaa aaatgataat tgccttaact gcctgacaat tccaacatca atgcactgat
    32401 aaaagatcag aatggtctaa ggcaggtctg aatgaatacc cagtataatt ccagttatat
    32461 attttcgatt accttagtcg ctacattagg tggtttatta tttggctacg acaccgccgt
    32521 tatttccggt actgttgagt cactcaatac cgtctttgtt gctccacaaa acttaagtga
    32581 atccgctgcc aactccctgt taggattttg cgtagccagc gctctgattg gttgcatcat
    32641 cggcggtgcc ctcggtggtt attgcagtaa ccgcttcggc cgtcgtgatt cacttaagat
    32701 ttctgctgtc ctgtttttta tttctggtgt aggttctgcc tggccagaac ttggttttac
    32761 ctctataaac ccggacaaca cagtacctat ttatctggca ggttatgtcc cggaatttgt
    32821 tatttatcgt attattggcg gtattggcgt tggtttagcc tcaatgcttt caccaatgta
    32881 tattgcggaa ctggctccag ctcatattcg cggaaaactg gtttcattta accagtttgc
    32941 gattattttc gggcaacttt tagtttactg cgtaaactat tttattgccc gttccggtga
    33001 tgccagctgg ctgaatactg acggcaggcg ttatatgttt gcctcggaat gtatccctgc
    33061 actgctgttc ttaatgctgc tgtataccgt gccagaaagt cctcgctggc tgatgtcacg
    33121 cggcaagcaa gaacagacgg aaagtatcct gcgcaaaatt atgggcaaca cgcttgcaac
    33181 tcaggcagta caggaaatta aacactccct ggatcatggc cgcaaaaccg gtggtcgtct
    33241 gctgatgttt ggcgtgggcg tgattgtaat cggcgttatg ctctccatct tccagcaatt
    33301 tgtcggcatc aatgtggtgc tgtactacgc gccggaagtg ttcaaaacgc tgggggccag
    33361 cacggatatc gcgctgttgc agaccattat tgtcggagtt atcaacctca cctttaccgt
    33421 actggcaatt atgactgtgg ataaatttgg tcgtaagcca ctgcaaatta tcggcgcact
    33481 cggaatggca atcggtatgt ttagcctcgg taccgcgttt tacactcagg catcgggtat
    33541 tgtggcgcta ctgtcgatgc tgttctatgt tgccgccttt gccatgtcct ggggtccggt
    33601 atgctgggta ctgctgtcgg aaatcttccc gaatgctatt cgtggtaaag cgctggcaat
    33661 cgcggtggcg gcccagtggc tggcgaacta cttcgtctcc tggaccttcc cgatgatgga
    33721 caaaaactcc tggctggtgg cccatttcca caacggtttc tcctactgga tttacggttg
    33781 tatgggcgtt ctggcagcac tgtttatgtg gaaatttgtc ccggaaacca aaggtaaaac
    33841 ccttgaggag ctggaagcgc tctgggaacc ggaaacgaag aaaacacaac aaactgctac
    33901 gctgtaatct tcctgtccag cacgccgcgc catttcggcg tgctgtcttt ttactcccgc
    33961 ttcagccgtt tcgaattaca cagccacagg gtgatcacca gtaacaggat cgcagccgag
    34021 tagatcaaca catccagtgg cgatttatga tcgacgatga tcaagcgcac aatggcggtg
    34081 atcccaatat agacaaagta acgtagcggg aagtgaaaac cggactgaaa gtacttcaca
    34141 atcagcgcga taaattcgaa atagagaaag taaaccacca gtccttctac cagctcatat
    34201 ttgctggttt gttctggcgc gaacagcaca tcagccagat gcaccgtttc tttgccgagg
    34261 aagacaacca aaatcaggcc gaggcacagc aggccaagat tgagtacggt ctgcaaaatg
    34321 gtggagataa actccacgcg cggacgagag agtgacgtca tagcagtacc tccttcacag
    34381 tggcgagctt cctgtataac gcaaaaatgt gacggagatc tatatttttt gtgtatgttt
    34441 tgttcaacct ggatttggag cgcgcctcca ccaaagccgg atatacctac ccgtaggccg
    34501 gataagccgc gcctggcggc gcatccggca agacaaaccg caccaaagcc ggtaattcaa
    34561 aaatcaccca ttcaggtgat tcccagccac cagcaatgcg cgaaaaatcg acaatgttcc
    34621 tctggaacaa atgaaacgta acctgataaa ggtggtaaaa atgaagcctt attttgctgc
    34681 tttgatgtta tcagtctctg ttcttcctgc ttatgctggt ccgctcggta ctgctgataa
    34741 agccgaccta ccgcaaagca atgtctccag cccaatgatg gcccagtcgc tcaggcaacc
    34801 agacctgcaa ccgatatcga cggacaggaa gacagaatgt tttcgccttt acacaccaga
    34861 tcgtaaaccg ggggtgaact gcgttcctga tggctcaaca ggtcattaag aatgagggtg
    34921 ccagtgagtg cgaaaactgg cacctttccc atgacttagc ggaaatttac gctacggtcg
    34981 ctggtcatgt catacaactg gaacttacgc ccaagttgct gaccaccgtc acgcgtcagc
    35041 ggcgtccagc caatcgccgc acggctgcgt gtcgggccag acgagaagag atccaacggt
    35101 accgagacat acacgccttt ggtgaagtcc ccttcgccgt actcctcttt cgaaacgttg
    35161 gtgatcgtgg catagccacc caccaccacg ccgctgtcaa agcgtttagc gatctccagc
    35221 gtgccgcctt tatcccctgc cagatactgc ccgacgctgg ctttcaccaa cacgtcctgg
    35281 gcgaaagatg gcgtccagta ggcggtcaga tgtccggttt tcacgctgta gtcggtgaat
    35341 ttcatcatat cttttgcact acgccagtcg cgctgtttaa cgtagttggc atccagacca
    35401 aacgcccagt tactgtccag cggacgatac aacacttcgg cccccgcacc gccaaacatg
    35461 gtttcgagat aacctccata gacctgaccg tagaagccgt tgcccagatg ctggaagtag
    35521 ttggcttgca ggttattcac atagacatcg ttctgcacat actcgcgcac atgggtacga
    35581 acgcgcggta agtgcgagtc ctgcggtgga ttggtgtagt taaacttgtc gtagttgttg
    35641 gcaagatttg caaacaggct gccggtggtc agtagatggt ccgtcagcca caaatctgcc
    35701 gttcccatca cgcccagctg atacatgtaa aagttttccg ggccaccgac cgactggttc
    35761 agcaccggat cgatatggaa atcaaagcgt gatttgtcga tataccagcc ctgctcggtg
    35821 gacttcggaa ccactggctc gacgcgtttg tgcgccagcg tcgtttcgtg gcccaacggc
    35881 tctccggcga gatgattttt caggctggcg acatcggttt ccgtcgtcac ttgcggcatg
    35941 ttaaggcgat tttccgtaat gcggatcgtt ttgatcccat ccggcagatc gttcatcacg
    36001 atccgattag cacggatgat cccttcgcgc gaatcacgat atttcacctg ctcgccggta
    36061 acatacagcg tatcgccttt cgcctggatc tgcggatcgg caagtccggc gttgtatttc
    36121 agcaacgtta actgattcgc caccaccgaa tgctgcaaaa tggcatcctg cggctgcggt
    36181 tgatattgcg ggcgggcgtt atcgatgtag gacgggcgca gatcgttaaa gttggtgcgc
    36241 agcgtgacgc caaacataaa ggtgttgcca cgttcatagc taaggttaac gtcggcccaa
    36301 tcggtaacgc gataaatcgc cccgacgtta aacttacttt tttgctccag cttcccggca
    36361 aaatcctgct gataattatt accttcatac tccagtttca gacgcagcgg ttgccagggc
    36421 gtctggtatt ccacgccgcc aaacagtgag gcaggaccgt ggaacatctg gctaccgtcg
    36481 atagatcccg cctgtttgta gctgttatcg cgatagcaat atttatcact ggctgaacag
    36541 agcggatttt ttacattacc gctggtgccc agatatcccc agcccaggcc gagcgtaaaa
    36601 tcgaacggcc cccaggcttt gctggcaaca agatattccg catcaaacag ccccgtaccg
    36661 ccgatatccc gcgcaccaac cgccacttgc ggcagccagt aactctcttc ccacaaacgc
    36721 agtttgagat cgaaggcttt atctttatac gtttgatcgc cagagaacgc ttcgacgctg
    36781 ctgtactgcc gggtgcgcac gtcggtgtag cgcagcgttg tttccagcca cgggaagagt
    36841 tgcactgaag ctgagtaata acggtactga tcgttatcgc gatagttcag actcaactcc
    36901 ccttcccgtg ccatacgcgc ggtgggagtt tgtaataatc ctacgccacc gaaatccgac
    36961 tgcgacggac caatgggcgc aggatatgtt tcgccgtagc aagctgtgct aatgcccagc
    37021 gccagtaagc taagcagatg tctttttttc attattgggg tatccgctgc gtcagagtct
    37081 gaagaatgtc ggcgttaagg gcatcaggcg tctcactcca gacggaatct gcgaggccaa
    37141 cataaataat gctgccgggc atcggctcta cgtgacgctt gttccagtaa gccaccggcg
    37201 ctttttgcgt gcgtccgtcc gggtaaacga cccacgcgta gctgcgatcc gcaccgctga
    37261 gcaggctctg gtcagagaga tagctcgcca cgtcgcgacc tggagtgaat ggctgattgc
    37321 caggatggct gataagcccg aacaacgtga ccgtggacgg cggtggtccg acccacagcg
    37381 tgtagttgcc ctgcaacggc gggttaccgc gttcggcaac gcggacgata tcgggatcaa
    37441 gattgatttt ttgcctgcct gtcactttca atgcctggat ttgctggcgt aaggcgttaa
    37501 tggcggcggc atcgtcggtg ctggaatctg cgccctgttc tgccagccgt gtcagcaacg
    37561 cctgctgctg acgcaatgct gccgccgttg ccagctcttc gctaatcacc gcaccaggcc
    37621 accagctgtt tgccagtcga ggttgcccta ccagatcgat taaatgctct gcgcccgtta
    37681 gcgttttggc ctcactgctg ccattgctga acaccttgac ggttcctgcc gcgaagaccg
    37741 atgacgctcc cacactcaaa agcaacgcga caatagtttg tttaatcatt gttttgccgc
    37801 cttgatgaga gtggttttca ccgggaaata atccgcgccg agatactgtt ccgactggcg
    37861 aatttgccct tcgctgtcga tccagtagcg gttatgccag cgcgcctggt cggtggagac
    37921 ttcttcgtcc agcacgcgaa ccagggtttc atcgctgccg accttgacgg tatcggtgcc
    37981 atcccatttg aagactgagc gtgcggtggc gtagcgtacc tgctggtact cggtccagcc
    38041 catcgtgcgc gtccatgttg caccatcaac aatttgtgcg ggtttaatca gcgggtcggc
    38101 ggcaaggtta ttcacttcaa tcaggttgtc gccgccaagc aaagtcttca ccagacgacc
    38161 atgttgtgtg acaagagtgg cttgatcctg agtgacccat ttttgttgtc cgtcttcagc
    38221 gaaggccagc accacaaaca attgcggccc gccattaagc tgcatgtact ggctggcgta
    38281 gggcatattt tgaatatcat cgtccgtcag ctgtacgcct ggcgtgccga acagactgtc
    38341 ccacagtgaa ttgcccagct ctttagtcgt ggctgaacag gcctgtaata gcaggcagat
    38401 aagaatgagt gcaggtcgct tcacgactct tctccagagg ggcagaataa ccacaccgga
    38461 gtgtggttat ggtaaatccc ttcatacttc aagccacaga tgcaatgact caaattattt
    38521 tggggataca tactttatta ctgggtactg gtggtcgtgg ttgtggtggt cccggtatta
    38581 gaaccatcac cgccaccggt tgccgcgagc gcgacaccca ccgccgagct tacggtgctg
    38641 acgctggtcg cggaggaact ccccgccgaa accgacgttg ctgccgactc tgccgcttcg
    38701 cccacctgta caggtgcagc ccacgcagaa gtcgccgcaa gcgcagatat ggcaaaaatg
    38761 ccatacagaa cttttttcat aacaatttcc cttcattgaa tgaatggaga tttacccaaa
    38821 aacatttcgg gcgggatcga gtatacacaa ctaaagcatg cgaattacgg cgcggggaat
    38881 tagcgatgga gttttaggat aattggaatg aatatcaaat ataagtttca acttatattt
    38941 aaataacaat aaaaatcata agtttaaaat aaaaaacaaa agtaattaat ctgcgtattc
    39001 tccgaaataa acaacgatta aaaatctatt ttaggattaa cctcacggta tgatttccgt
    39061 taaatcacag acaaggggat ttatctgata aaacaatgcc gacttattgt cggcattgtt
    39121 tttaaggtta tttaactcct catactatgc attaatgtcg atattggcat caggctccgg
    39181 ttgccggatg cggcgtaaac gccttatccg gcctacatat cggcatcagg cacgcatgat
    39241 gattaaccgc gccacgcttt atagcggtta atcagaccat tggtcgagct atcgtggctg
    39301 ctgatttctt tatcatcttt cagctctggc agaatacggt ttgccagttg tttacccagc
    39361 tccacgcccc actggtcgaa ggtgaagatg ttcaggatca cgccctgagt aaagattttg
    39421 tgctcataca gcgcaatcaa cgcacccagg ctgaacggag tgatttcgcg cagcaggatg
    39481 gagttggtcg ggcggttacc ttcgaacact ttgaacggca ccacgtagtc aagcgttgct
    39541 ggatctttac cctgatcgcg atattcctgc tcaaccactt cgcgggattt accaaacgcc
    39601 agcgcttcgg tctgggcgaa gaagttagac agcagtttct ggtggtgatc agagagcggg
    39661 ttatgggtga tagccggggc gatgaaatcg cacggtacca ttttggttcc ctggtggatc
    39721 agctggtaga acgcgtgctg accgttagtg cctggttcac cccagataat cgggccagtc
    39781 tggtaatcca caacgttacc gttacggtca acatacttac cgttggactc catattgccc
    39841 tgctggaagt acgccgcgaa acggtgcata tactggtcat acggcagaat cgcttcagtt
    39901 tccgcaccaa agaaattgtt gtaccagatg ccaatcagcg ccagcaggac aggcaggttt
    39961 ttctcggcag gcgtggtgga gaaatgcttg tccattgcgt gtgcgccaga aagcagttca
    40021 acgaagttat caaagccgat ggagagaaca atcgacaggc caatcgctga ccacaaagag
    40081 taacggccgc cgacccagtc ccagaactcg aacatgttgg cagtatcaat accaaactcg
    40141 ccaacggctt tggcattggt tgagagcgcc gcaaaatgtt tcgcaacgtg tttttcatca
    40201 cctgccgctt tcaggaacca gtcacgcgcg ctatgggcgt tggtcatggt ttcctgagtg
    40261 gtgaaggttt tagatgctac caggaacagc gtggtttccg ggtttacttt tttcagcact
    40321 tccgagatgt gagtcccatc gacgttagaa acaaagtgca tgttcaggtg gtttttgtac
    40381 ggacgcagtg cttcggtcac catgtacggg ccgaggtcag aaccgccgat cccgatgttc
    40441 actacgtcag tgattgcttt gccggtataa cccttccact caccggagat aatcgcttcc
    40501 gagaaggttt tcatcttctc cagcaccgcg ttgacttctg gcataacatc tttgccatca
    40561 accagaatcg gggtattgct acgattacgc agcgctacgt gcagcacggc gcggttttca
    40621 gtgcggttga tcttctcgcc agagaacatc gacttaatcg cgcccgccag atcgcactct
    40681 ttcgccagat cctgtaattt cgccagcgtc tcttcagtga tgcggttttt ggagtaatcc
    40741 accagcatct gatcgtcgaa ggttgcggag aacttagaaa aacgatcgcc gtctttagca
    40801 aaaagatcgg cgatcgtaac gtctttcatt tcatcgaagt gtttctgtag tgcctgccag
    40861 gcagcggtct gcgttggatt gatgtttttc attagcaata ctcttctgat tttgagaatt
    40921 gtgactttgg aagattgtag cgccagtcac agaaaaatgt gatggtttta gtgccgttag
    40981 tgtaatgttg agtgtaaacc cttagcgcag tgaagcattt attagctgaa ctactgaccg
    41041 ccaggagtgg atgaaaaatc cgcatgaccc cgtcgttgac aaccgccccg ctcacccttt
    41101 atttataaat gtactacctg cgctagcgca ggccagaaga ggcgcgttgc ccaagtaacg
    41161 gtgttggagg agccagtcct gtgataacac ctgagggggt gcatcgccga ggtgattgaa
    41221 cggctggcca cgttcatcat cggctacagg ggctgaatcc cctgggttgt caccagaagc
    41281 gttcgcagtc gggcgtttcg caagtggtgg agcacttctg ggtgaaaata gtagcgaagt
    41341 atcgctctgc gcccacccgt cttccgctct tcccttgtgc caaggctgaa aatggatccc
    41401 ctgacacgag gtagttatgt ctgaaattgt tgtctccaaa tttggcggta ccagcgtagc
    41461 tgattttgac gccatgaacc gcagcgctga tattgtgctt tctgatgcca acgtgcgttt
    41521 agtagtcctc tcggcttctg ctggtatcac taatctgctg gtcgctttag cggaagggct
    41581 ggaacctggc gagcgattcg aaaaactcga cgctattcgc aacatccagt ttgccattct
    41641 ggaacgtctg cgttacccga acgttatccg tgaagagatt gaacgtctgc tggagaacat
    41701 tactgttctg gcagaagcgg cggcgctggc aacgtctccg gcgctgacag atgagctggt
    41761 cagccacggc gagctgatgt cgaccctgct gtttgtcgaa atcctgcgcg aacgcgatgt
    41821 tcaggcacag tggtttgatg tacgtaaagt gatgcgtacc aacgaccgat ttggtcgtgc
    41881 agagccagat gtagccgcgc tggcggaact ggccgcgctg cagctgctcc cacgcctcaa
    41941 tgaaggctta gtgatcaccc agggatttat cggtagcgaa aataaaggtc gtacaacgac
    42001 gcttggccgt ggaggcagcg attatacggc agccttgctg gcggaggctt tacacgcatc
    42061 tcgtgttgat atctggaccg acgtcccggg catctacacc accgatccac gcgtagtttc
    42121 cgcagcaaaa cgcattgatg aaatcgcgtt tgccgaagcg gcagagatgg caacttttgg
    42181 tgcaaaagta ctgcatccgg caacgttgct acccgcagta cgcagcgata tcccggtctt
    42241 tgtcggctcc agcaaagacc cacgcgcagg tggtacgctg gtgtgcaata aaactgaaaa
    42301 tccgccgctg ttccgcgcgc tggcgcttcg tcgcaatcag actctgctca ctttgcacag
    42361 cctgaatatg ctgcattctc gcggcttcct cgcggaagtt ttcggcatcc tcgcgcggca
    42421 taatatttcg gtagacttaa tcaccacgtc agaagtgagc gtggcattaa cccttgatac
    42481 caccggttca acctccactg gcgatacgtt gctgacgcaa tctctgctga tggagctttc
    42541 cgcactgtgc cgggtggagg tggaagaagg tctggcgctg gtcgcgttga ttggcaatga
    42601 cctgtcaaaa gcctgcggcg ttggcaaaga ggtattcggc gtactggaac cgttcaacat
    42661 tcgcatgatt tgttacggcg catccagcca taacctgtgc ttcctggtgc ccggcgaaga
    42721 tgccgagcag gtggtgcaaa aactgcatag taatttgttt gagtaaatac tgtatggcct
    42781 ggaagctata tttcgggccg tattgatttt cttgtc
//
LOCUS       DAJTSF010000024        40544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000024 DAJTSF010000000
VERSION     DAJTSF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.024"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..956)
                     /locus_tag="OO335_001391"
     CDS             complement(<1..956)
                     /locus_tag="OO335_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR6001840.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="OO335_001392"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="OO335_001392"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR6001841.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OO335_001393"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OO335_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OO335_001394"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OO335_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6001842.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="OO335_001395"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="OO335_001395"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6001843.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="OO335_001396"
     CDS             complement(5018..6346)
                     /locus_tag="OO335_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6001844.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8696)
                     /locus_tag="OO335_001397"
                     /pseudo
     CDS             complement(6354..8696)
                     /locus_tag="OO335_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="OO335_001398"
     CDS             complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="OO335_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6001845.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="OO335_001399"
     CDS             complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="OO335_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6001846.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="OO335_001400"
     CDS             complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="OO335_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6001847.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="OO335_001401"
     CDS             complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="OO335_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6001848.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="OO335_001402"
     CDS             complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="OO335_001402"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6001849.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15311..16546
                     /gene="moeA"
                     /locus_tag="OO335_001403"
     CDS             15311..16546
                     /gene="moeA"
                     /locus_tag="OO335_001403"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6001850.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16546..17295
                     /gene="moeB"
                     /locus_tag="OO335_001404"
     CDS             16546..17295
                     /gene="moeB"
                     /locus_tag="OO335_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6001851.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="OO335_001405"
     CDS             complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="OO335_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6001852.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18267..19166
                     /locus_tag="OO335_001406"
     CDS             18267..19166
                     /locus_tag="OO335_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6001853.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19172..21604
                     /locus_tag="OO335_001407"
     CDS             19172..21604
                     /locus_tag="OO335_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6001854.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21750..22565
                     /gene="ybiV"
                     /locus_tag="OO335_001408"
     CDS             21750..22565
                     /gene="ybiV"
                     /locus_tag="OO335_001408"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6001855.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22717..23982
                     /locus_tag="OO335_001409"
     CDS             22717..23982
                     /locus_tag="OO335_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6001856.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24223..25815)
                     /locus_tag="OO335_001410"
     CDS             complement(24223..25815)
                     /locus_tag="OO335_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6001857.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26034..26954
                     /gene="ldtB"
                     /locus_tag="OO335_001411"
     CDS             26034..26954
                     /gene="ldtB"
                     /locus_tag="OO335_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6001858.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27012..28130)
                     /locus_tag="OO335_001412"
     CDS             complement(27012..28130)
                     /locus_tag="OO335_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6001859.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="OO335_001413"
     CDS             complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="OO335_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6001860.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28780..28908
                     /gene="mntS"
                     /locus_tag="OO335_001414"
     CDS             28780..28908
                     /gene="mntS"
                     /locus_tag="OO335_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6001861.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29180..30763
                     /gene="opgE"
                     /locus_tag="OO335_001415"
     CDS             29180..30763
                     /gene="opgE"
                     /locus_tag="OO335_001415"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6001862.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="OO335_001416"
     CDS             complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="OO335_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6001863.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31379..31444)
                     /locus_tag="OO335_001417"
     CDS             complement(31379..31444)
                     /locus_tag="OO335_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001864.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31679..32566
                     /gene="rhtA"
                     /locus_tag="OO335_001418"
     CDS             31679..32566
                     /gene="rhtA"
                     /locus_tag="OO335_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6001865.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32865..33368
                     /gene="dps"
                     /locus_tag="OO335_001419"
                     /gene_synonym="pexB"
     CDS             32865..33368
                     /gene="dps"
                     /locus_tag="OO335_001419"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6001866.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33772..34518
                     /gene="glnH"
                     /locus_tag="OO335_001420"
     CDS             33772..34518
                     /gene="glnH"
                     /locus_tag="OO335_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6001867.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34657..35316
                     /gene="glnP"
                     /locus_tag="OO335_001421"
     CDS             34657..35316
                     /gene="glnP"
                     /locus_tag="OO335_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6001868.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35313..36035
                     /gene="glnQ"
                     /locus_tag="OO335_001422"
     CDS             35313..36035
                     /gene="glnQ"
                     /locus_tag="OO335_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6001869.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36296..38520
                     /gene="ybiO"
                     /locus_tag="OO335_001423"
                     /pseudo
     CDS             36296..38520
                     /gene="ybiO"
                     /locus_tag="OO335_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="OO335_001424"
     CDS             complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="OO335_001424"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6001870.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39719..39979
                     /locus_tag="OO335_001425"
     CDS             39719..39979
                     /locus_tag="OO335_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6001871.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40244..>40544
                     /locus_tag="OO335_001426"
     CDS             40244..>40544
                     /locus_tag="OO335_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6001872.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT        10098 a        10718 c         9865 g         9863 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatctttt cgtataaact cagcatgtta
     8701 atacatttaa caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc
     8761 ggctttttaa ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta
     8821 gggcaataat ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct
     8881 ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg
     8941 gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct
     9001 cgtgcctcat tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga
     9061 aatgacaggc tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg
     9121 atagaagaga cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca
     9181 atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc
     9241 aggcgggcaa acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg
     9301 cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca
     9361 tcgcaaatgc gcatgatcag ccgatcccac cagccttcat aatatccagc caacaagccc
     9421 agcaacgtcc ctatcgccac accgataaac acggcaaaca cgcccgccgc cagcgagatt
     9481 tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag
     9541 tgctgcaaag aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag
     9601 ggagcgatcc agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg
     9661 gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc
     9721 tggtcaggtt tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt
     9781 cgcatcctta cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta
     9841 agttgataag aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat
     9901 cgcgcatttc tacagagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct
     9961 ccaccacgat agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg
    10021 ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg
    10081 ccctcgcggt acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc
    10141 gcgccatcac ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc
    10201 aactgtctgc tcccacagta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa
    10261 acatccccag cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc
    10321 gatccggcca acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca
    10381 ccgcccagac catactggtt atggtcagcc atagcgttgg cataaagcgg ctggcaatct
    10441 catcggcaac cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc
    10501 tgatatagtg ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca
    10561 gttctataac ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca
    10621 gcatgtggac aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca
    10681 gtaatcgttt gataacgtaa taaagcattc cactccctgc gttgcttatt gcaaatccgc
    10741 gtcttcaaag ctgaagccgg tatctggcat gatccaaaaa ccggtcaggt ttttactgtg
    10801 tgccgacacc agtttttcta ccaccagcgg gatccacggc gattcttgcc agatgatatc
    10861 ctgcgccgcc ttatataagc gggtcttttc cgccggatca ttcgttttca gtgcctgagc
    10921 caggaagtca tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca
    10981 gttttgcgag gcaaacagcg gcgatagtgc ccagtcggct tcgccggttg aagccgacca
    11041 gccagtgtag aacatccgca cgccactctc tttttgccct ttaccttcaa cttctgccgc
    11101 ccgctgtccg gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg
    11161 ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt
    11221 actgaaaccg ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta
    11281 aggccacggt ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg
    11341 agttgcatag cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag
    11401 cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg
    11461 ctgcataatt gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc
    11521 ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg
    11581 ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg
    11641 ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc
    11701 atacggtccg gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgt
    11761 cggtgaaatc atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg
    11821 tttgagggta atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata
    11881 caggttatag cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac
    11941 cgccactgcg ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt
    12001 gtaagtaatg ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat
    12061 ctctttatcc agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt
    12121 gtcattcgcg tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc
    12181 tttggcagcg aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc
    12241 actacggtgt acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg
    12301 cgtattctga ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg
    12361 ctgccacctc ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac
    12421 gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat
    12481 aaggatgctg cgggttttcg aagaccgcgc accgtgaacc aatttcaaca atttgcccga
    12541 gatacatcac cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa
    12601 acagatacgc aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc
    12661 gaatagaaac atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg
    12721 ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac
    12781 gccaggcatg ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg
    12841 cgtctttacc tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg
    12901 tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca
    12961 atgcctgaag tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc
    13021 cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag
    13081 actcgcccac cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg
    13141 catgcacttc ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa
    13201 gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt
    13261 cctgtttcgc tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt
    13321 tcatcgcacc aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg
    13381 catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta
    13441 cccgatcggc aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg
    13501 acatctcttt ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca
    13561 gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa
    13621 tcatcactcg ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt
    13681 gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac
    13741 gactggcgtt ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg
    13801 ggttcagcga tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc
    13861 gcatttgtgc agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca
    13921 gcattttatc gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg
    13981 cagtcactga cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca
    14041 gactaaaaga gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat
    14101 taagattttc aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg
    14161 tccccttttt cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga
    14221 tacattcctt cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag
    14281 ccaccgctac cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg
    14341 agacttaatc cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg
    14401 aagacttcgc ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg
    14461 gcacccacta agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg
    14521 gacgtggctg ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt
    14581 ttctgttttt catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc
    14641 gctgccagta gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc
    14701 atgccatgag caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt
    14761 tgctccatca ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc
    14821 gcaccggctt tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca
    14881 cgcgtaaaga cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc
    14941 gcttccgtca ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca
    15001 acaatggcag acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg
    15061 ctaattgcac ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat
    15121 taagggtggc tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat
    15181 ttttgaatat agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg
    15241 tggcatacag tctatggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag
    15301 gagtgttttc atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat
    15361 gctttctcgc gttaccccac tgaccaccca ggaaacgctg ccactggttc agtgttttgg
    15421 tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc
    15481 tatggacggc tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc
    15541 cggtaaatcc tttgccggtc agccatacca tggtgaatgg cctgcgggta cctgcattcg
    15601 tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac
    15661 tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg
    15721 ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac
    15781 taccgcagag ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa
    15841 agtgcgtgta gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg
    15901 cgacggccaa atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg
    15961 ttgcgaggta atcaacttag ggattatccg cgacgatcca catgccctgc gcgccgcatt
    16021 tattgaagcc gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga
    16081 agcggattac accaaaatga ttcttgaaga gctgggggag atcgccttct ggaagctggc
    16141 gattaaacca ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct
    16201 gccgggcaac ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc
    16261 aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc
    16321 ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc
    16381 cgacggtgaa cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt
    16441 tagtctcggc aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgcgcga
    16501 atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc
    16561 gatcaggaga tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt
    16621 caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca
    16681 gcttcgcagt atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg
    16741 gtttcgctct cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg
    16801 aaggtggaat ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca
    16861 gtcaatgcac tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg
    16921 ctcgactgta cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg
    16981 aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact
    17041 tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta
    17101 acctgcgtag aagcaggcgt gatggcaccg ttgataggcg taattggttc gttgcaggca
    17161 atggaagcga tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg
    17221 tacgatgcga tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag
    17281 gtgtgcgggc agtaattgcc acctccaagc ctgataagac gcgacaagcg tcgcatcagg
    17341 catttgctcc agacgccaga tgcggcgtga acgccttatc tggcctacgg ttcgggcaca
    17401 gatttccaag cgcggcaggc atcggtgcac tccgccacgt catcagcaat ttatgaggat
    17461 gcagaacgtc cggttaaatc gacgttctgc caaacgctcc ctgccagtcc tgctcaaact
    17521 tcgtcacagc ggcttccacc gccggatagc taatcatctg ttgtgccaca tccagtggca
    17581 gagtaattga ttcacatccg gccagtaagc agtccagcgc ctgacgcggg gttttgaaac
    17641 tcgctgccag cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca
    17701 cagtctgaat gccgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt
    17761 ccgcacctgc cagcgccgac agcagccctt gtgccgcgcc atataccgcg gttcccagcg
    17821 tcggaatccc ttccgctttt aacatcttaa tcgctgccag cccctcggct gtcaccggaa
    17881 ctttcaccac gatatccgca ataatagaac gcagcttaag cgcgtcattc accatccctt
    17941 cagcagtggt agccattacc tgggcaaaca gacgcccctg accgcccatc gcttcatgaa
    18001 gttgcggaag cacaacatcc agcggttttt tacccgcggc gataatgctt gggttagtgg
    18061 tcacacccgc cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat
    18121 ccagatacag ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac
    18181 gggaaaactc tctgaacagt tgacgcacat caatctaact ttcattcgaa agtattttaa
    18241 tgtttgaacg aaacagagag gcaactatga ttttcaatat tcagcgctac tcgacccatg
    18301 atggccccgg tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt
    18361 gtcagaaccc ggaaagccgc gcccgtacgc aggatctgct gtatgacgca cgactgtgtc
    18421 tggaaggctg cgagctgtgc gctaaggccg cgccggaagt gattgagcgc gcgctgaatg
    18481 gtttgcttat tcatcgggaa aagttaaccc cggagcatct gacggcgtta accgactgct
    18541 gtccgacaca ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcga
    18601 ccgttctgcg cgataaaccg ttttacgatc gcagcggcgg cggcttaacg ctttcgggtg
    18661 gtgaaccctt catgcagccg gaaatggcga cggcgctgct gcaagcctgc cacgaggcag
    18721 gcattcatac tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc
    18781 tgccctatat cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac
    18841 agtggaccga cggtaacgcc gccagggtgc tggataacct gaaaaaactc gccgcagcgg
    18901 gcaaaaaaat cattatccgc gtgccgctga ttcagggctt taatgccgac gaaacctctg
    18961 taaaagccat taccgatttt gccgccgacg agctgcacgt tggcgaaatt cattttctgc
    19021 cctaccacac gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg
    19081 aaaaaccgct tgatgcgcca gagctgctcg attttgccca gcagtatgcc tgccagaaag
    19141 ggttaaccgc gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag
    19201 cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt gtgaagccgc cagtctgtac
    19261 cgagcgcgcg cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt
    19321 acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacacga
    19381 tgagttgatc attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata
    19441 tactgtctcg tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggctt
    19501 tgcggtgagc gaagagaaca aacgtgttct gcatgaagtg tgcccgtggt ggcgcggtca
    19561 gacggtacag gatcgctgct atggcatgtt taccgatgag caaaaaggtc tgctggcgac
    19621 cggcatcatt aaagcggaag gcaatatgac ctccggcgat gcgcacctgg cggtcaattt
    19681 cccgctgctg ctggaaaaag ggcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc
    19741 gcgcatcaat ctgacggtgc tggaagattt gcacggcgaa cagttcctga aagcgattga
    19801 tatcatgctg gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgcaat
    19861 ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg ctggcgatgg cagaaaactg
    19921 cgatcttatc gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt
    19981 catccagttg attttgcaga ttgaatctaa cggtcactcg gtgtcgtttg gtcgtatgga
    20041 tcagtatctc tacccgtact atcgccgcga cgttgaactg aaccagacgc tggatcgcga
    20101 acacgccatc gaattgctgc atagctgctg gttgaaattg ctggaagtga acaaaatccg
    20161 ctccggctcc cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg
    20221 cggacaaaat ctggttgatg gtcaaccaat ggacgcggtg aatccactct cttacgcgat
    20281 cctcgaatcc tgcggtcgcc tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc
    20341 aggaatgagt aacgatttcc tcgacgcctg cgtacatgtg atccgctgcg gcttcgggat
    20401 gccggcattc aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc
    20461 gcaggacgct tatgactacg cagcgattgg ttgtatagaa accgccgtcg gtggcaaatg
    20521 gggctatcgc tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct
    20581 ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc
    20641 ggcaggtaac ttcaacaact tcgatgaagt gatggacgcg tgggatacgc aaatccgtta
    20701 ctacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt
    20761 gcacgatatt ctctgctcgg cgctggtgga tgactgtatt gagcgagcga aaagtatcaa
    20821 gcaaggcggc gcgaaatatg actgggtttc tggcctgcag gtcggtattg ccaacctcgg
    20881 caacagcctg gcggcagtga agaaactggt gtttgaacag ggcgcgattg gtcagcaaca
    20941 gctggctgcc gcactggcgg atgacttcga tggcctgact catgagcaac tgcgtcagcg
    21001 gctgattaac ggtgcgccga agtacggcaa cgacgatgat accgtcgata cgctgctggc
    21061 tcgcgcttat cagacctata tcgacgaact gaaacagtac cataacccac gctacggtcg
    21121 tggtccggtt ggcggcaact attacgcggg tacgtcgtct atctccgcta acgtaccgtt
    21181 tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg
    21241 cgctagtccg gcctctggta ctgaccatct cggccctact gcggtcattg gctcagtggg
    21301 taaactgcct acggcagcga ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc
    21361 aacgctggag aacgaatctg acaagcagaa actgatgatc ctgctgcgta ccttctttga
    21421 agtgcataaa ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc
    21481 gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt
    21541 cttcaccgcg ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct
    21601 gtaatctctt cggcccggct gcggcgtcgc cgggctaaat cattcacatc atcaatttca
    21661 tccttacttt cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac
    21721 ggtttcattt tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagatatgg
    21781 acggtacttt tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc
    21841 aggaactgaa aaagcgcggc attaagttcg ttgtcgccag cggtaatcag tattaccagc
    21901 ttatttcgtt ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac
    21961 tggtttacga acatggcaag caactgttcc acggcgaact gacccgacat gaatcgcgga
    22021 ttgttattgg cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa
    22081 gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc actgatggca aaacactacc
    22141 atcgcctgaa acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc
    22201 tcaacctgcc ggatgaacaa atcccgttag tgatcgacaa actacacata gcgctggatg
    22261 gcattatgaa acccgtcacc agtggttttg gctttatcga cctgattatt cccggtctac
    22321 ataaagcaaa cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg
    22381 tagcgattgg cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg
    22441 cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata
    22501 atcatgaagg cgcgctgaat gtgattcagg ctgtgctgga taacacatcc ccttttaaca
    22561 gctgacctct ctaccggagc catgcgctcc ggttttccct tctttttaca tcctgtacat
    22621 cacatcaaat cattaaactt gcattaactt atttataact tgtattgtca catgcaaatt
    22681 agcatagttt cgaatgaaag ttaatgcaag gtggttatgg cttctacttt taccagcgac
    22741 acattgcctg ccgatcacaa agcagctatc cgtcagatga agcacgcgct gcgggcgcag
    22801 cttggcgatg tccagcagat ctttaatcag ctaagcgatg acattgccac gcgagtggct
    22861 gaaatcaacg cactcaaagc acagggcgat gccgtctggc cggtgctgtc ttatgccgat
    22921 atcaaagcag gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg
    22981 gtgataaaag gccatttccc ccgcgaacaa gcgctaggct gggatcagtc gatgctggac
    23041 tatctgaacc gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg
    23101 acgctcagcg cttcacgtcc cgagatttac cccatctact ggtcgcaggc gcaaatgcag
    23161 gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt
    23221 gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc
    23281 cgccgccttc cgcccggaac gacctccaaa ggtcttggag cgcataccga ctccggggcg
    23341 ctggaacgct ggctgcttcc agcgtatcag cacgttttcg ccaacgtctt taatggcaat
    23401 ctggcgaaat acgatccctg gcatgcggca catcgtacgg aagttgaaga gtacacggtg
    23461 gacaacacca ccaaatgttc cgtgtttcgg acattccagg gctggactgc gctctcggat
    23521 atgctgcctg gtcaggggtt gctgcacgtc gtgcccattc ctgaagccat ggcgtacgta
    23581 ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga
    23641 agagtgttgc cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt
    23701 ccaaaactcg aggccggaga ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc
    23761 cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc
    23821 gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg
    23881 ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag gacgctttac gcttgccgac
    23941 ctcaacattc acggtaagcg agcgttgggc atggatgttt gagagttaat ggaagtgaat
    24001 tgatgtggga ttaagaaaat cgattcgacg ttactcagat tgctgacaag tgcgctttct
    24061 taatgcctga tgcggcgtta acgccttatc cggcctacaa agtcctgcag attcaatata
    24121 ttacaggtac tgcgtaggcc tgataagcgt agcgcatcag gctattttgc atttgtcatc
    24181 agccgtcgtc accccgacag tatcggggtg acggataatc acttactcga tccctttact
    24241 acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg cgttccgggg tgatttccag
    24301 aatgcgggtc gccagggagc ttacgaactc acggtcgtgt gaaacaaaga tcagcgtgcc
    24361 ctgatacagt tccagcgcca tgttcagtga ctcgatggat tccatatcca ggtggttggt
    24421 cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt aacttaccaa acagcatccg
    24481 ccctttttcc ccaccggaaa gcactttagc tggctttttg atgtcgtcct ggctgaacag
    24541 caaacgaccg agaatgctgc gtaccgcctg ctcgtcatcg ccttcctgct tccactgact
    24601 catccattcg aacacggtca gatcattttc aaactcatat tcgtggtcct gggcgtagta
    24661 accaatgcgc gcgttttcag accattttac ggtgccgctg tccggttgca gatcgcccac
    24721 cagcgttttc agcagcgttg atttaccgac gccgttggta cccagtaccg caagtttttc
    24781 accgacctcc agcagcaggt tgagattttt aaacagtgga ccgttatcaa accctttggt
    24841 cagaccttcc acttccagcg cgttacggaa cagtttctta tcctgttcaa aacggataaa
    24901 cgggttctga cggctggagg ctttcacctc ttccagtttg attttatcaa tctggcgcgc
    24961 gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta aagcggctaa cgaaagattg
    25021 caactcagca atctgcgctt tcttcttggc gttatcggcc agcagacgtt cacgcgcttg
    25081 ggtcgccgcc gtcatgtact catcgtagtt acccggataa acgcgcagct cgccgtaatc
    25141 cagatccgcc atgtgggtac agaccatgtt aaggaagtga cggtcgtgcg agatgatgat
    25201 catggtgctg tcacgctcgt tcagcacctg ttccagccag cgaatggtgt cgatgtcgag
    25261 gttgttggtc ggttcgtcga gcaggagaat atccggctca gcaaacagcg cctgcgccag
    25321 aagcacacgc agcttccagc caggagcaac ttcactcatc gggccgtagt gctgttccac
    25381 tggaattccc acgccaagca acagttcacc ggcgcgagct tccgcagagt aaccgtccat
    25441 ttcgccgtat ttcacttcca gatcggccac tttatagccg tcttcttcac tcatttccgg
    25501 caaagcatag atgcggtcgc gctcctgctt cacttcccac aactctttat gccccatgat
    25561 caccgtatcc agcacagtga actcttcaaa ggcaaactga tcctgacgca gcttaccaat
    25621 gcgctcgttg ggatcgaggg aaacgttacc cagcgtcggc tcaaggtcgc cgccgagaat
    25681 cttcataaag gtggatttac cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc
    25741 gccgaatttg acggaaatgt tttcaaacaa cggcttactg ccgaactgca tggtgacgtt
    25801 actggaaact aacacaggcg tatcctgaaa agagatatga caaaccgcgc attatgccat
    25861 aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa
    25921 tgtgatttcg tacacatctg atttcactgt gagctggaat gaacttataa tgcgcttcca
    25981 atactctaat attctcaacc caatggcctg ccaggcacaa aatctcgctt aacatgaata
    26041 tgaaattgaa aacattattc gcagcggcct tcgctgttgt cggcttttgc agtaccgcct
    26101 ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga
    26161 tcaccattcc tgaaggtaac actcagccgc tggagtattt tgccgcggag taccagatgg
    26221 ggctttccaa tatgatggaa gcgaacccgg gtgtggatac cttcctgccg aaaggcggta
    26281 ctgtactgaa cattccgcag cagctgatcc tgccggatac cgttcatgaa ggcatcgtca
    26341 ttaacagtgc agagatgcgt ctgtattact atccgaaagg gaccaacacc gttatcgtgc
    26401 tgccgatcgg cattggtcag ttaggcaaag atacgcctat caactggacc accaaagttg
    26461 agcataagaa agcaggcccg acctggacgc cgaccgccaa aatgcacgca gagtaccgcg
    26521 ctgcgggcga accgctaccg gctgtcgttc cggcaggtcc ggataacccg atgggtctgt
    26581 atgcactcta catcggtcgc ctgtatgcta tccatggcac caacgccaac ttcggtatcg
    26641 gcctgcgtgt aagtcatggt tgtgtgcgtc tgcgtaacga agacatcaaa ttcctgttcg
    26701 agaaagtacc ggtcggtacc cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg
    26761 agccagacgg cagccgttat attgaagtcc ataacccgct gtctaccacc gaagcccagt
    26821 ttgaaggtca ggaaatcgtg ccaattaccc tgacgaagag cgtgcagaca gtgaccggtc
    26881 agccagatgt tgaccaggtt gttcttgatg aagcgatcaa aaatcgttcc gggatgccgg
    26941 ttcgcctgaa ttaatcgtca aaacgtaaaa caaaggcgga ctcataatcc gcctttttta
    27001 tttgccagac cttaccaggc cgggatcata actaacaaaa cgtatcccac cagcgccgcc
    27061 cacaacagca tcggtattga atagagatgg aaacgccacc agatgcggcg atcgttcgcc
    27121 atacgtagcg caatcaaatt tgccagcgat cctggtaata aaccaaagcc ccccacgttt
    27181 accgcccatg ccagtaataa agacggaggt acataattga gcagcaaaat ggtactcggc
    27241 acgttactga tcacctgcga taaaccgact gccgttaacc ataacccggg ttcagataaa
    27301 tgactcacgt tacccaacac gccttgcaac gctggaagct gggtcagtaa atggacgtcg
    27361 ataaacatcg ccataaacac cagcagcaac gtccagtcca cactcaacac cacgcgacgc
    27421 gccagcagca caaagcccgc cgccacaatc accagtcccc acagctcttg tttgaactcc
    27481 agcgccgtca gaaagacgat atacagcccc agacaattcc acaccagccg cggtttccac
    27541 tccggtgttt gcacccccgt atggtattgc agcgcctttc cagggaaaca acaccagcac
    27601 aacagcagga gtgtcagcat cattgcgcca gccatcggtg ccatttgggc aataaatccg
    27661 gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc aattggcgtc
    27721 agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt aaccgggatc
    27781 tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc gacatcgttg
    27841 gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag ccgacgctcc
    27901 gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct taactccaca
    27961 cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc gatagcagca
    28021 ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac aagaattaat
    28081 aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat ttggcaccgt
    28141 gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc actaacatgg tgctccatgc
    28201 cttccgcgtc gcgacgggcg atttccggac tgacgcccaa caccaacaag aaattttcga
    28261 ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc gttaaaaaca
    28321 cgccacgcca ggggatcatt tcaatcagcc ccatggttgc cagccgctta agcattttag
    28381 ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc cacctgacga gcttccccca
    28441 cttccctgat caagtcagaa atcagctcaa cgtaatcatc aataagctcg cgccgatgcg
    28501 cctctctgac ctggcggaac ccttcaacgt gctcttccac gttcactaac tgcgtcactt
    28561 tttttgctat tggcgtacct gcgcgacgac tcattgtgct tcctcattat ggtgacgcat
    28621 acatagcgtt ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt acaaaaaatt
    28681 aacgttttag caatagctat ataatatagc ctgtgctata tctgtatgta atgcaatcat
    28741 ccctcaagga tcgacgggat tagcaagtca ggaggtctta tgaatgagtt caagaggtgt
    28801 atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa tgctgctctc tatgttgtgc
    28861 gatatggtca acaacaaacc gcagcaagat aaaccttcag ataaatagcg gcgtcgcggt
    28921 acgccgcttc actcctgctt ttatgcaggc ataacgcgtt ttggtctgaa aaaaaccact
    28981 ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt gacctgaacg
    29041 gcggctcgct ctatcttctt gcagccctgc gtatattgcg gctcgcggat gcggacccct
    29101 ttcccctctt cacgcactct tgcaggtatt gacccttgac gccagggtaa gcacatggcg
    29161 tttgttacga tagtggcata tgaatttaac cctcaaagaa tcgcttgtta cccgtagccg
    29221 ggtatttagc ccgtggactg cgttctactt tttacagtcg ctattaatta acctcggctt
    29281 aggttacccc ttcagtttgc tctacaccgc tgcgtttacg gctattttgc ttttgctatg
    29341 gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc agttcgctgg tggcggcttg
    29401 ttatttccct tttgctcagg cctacggcgc gcctaatttc aatacattgc tggcattgca
    29461 ctccaccaat atggaagagt cgaccgaaat cctgacgatt tttccgtggt acagctacct
    29521 ggtcggctta tttatttttg cgctcggcgt aatagcaatc aggcgaaaaa aagagaatga
    29581 aaaagcgcgc tggaatacct tcgacagcct gtgtctggta ttcagtgtgg cgacattttt
    29641 tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag atactggcta
    29701 tccggtattt cgttttgcta aggatgtcat cgtcaataat aacgaggtga ttgaagagca
    29761 agaacggatg gcaaaacttt ccggaatgaa agatacctgg acggtcactg ccgttaagcc
    29821 gaagtatcag acctatgtgg tagtgatcgg tgaaagcgcg cgtcgcgatg ccctcggtgc
    29881 ctttggcggt cactgggaca ataccccgtt tgccagcagc gttaacggtt tgatatttgc
    29941 tgactacatt gctgccagtg gctccacgca gaaatcgctt ggcttaacgc tcaatcgcgt
    30001 agtcgatggc aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc gcgcgggctt
    30061 ccagacctgg tggttttcca accagggcca aatcggcgaa tacgatactg ctatcgccag
    30121 catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa ggtaattttg aagcagataa
    30181 aaacacgaaa gacgaagcgt tactggatat gaccgctcaa gtgctggcgc aagagcactc
    30241 gcaaccgcag ctgattgttc tgcatctgat gggctcgcat ccgcaggcct gcgacaggac
    30301 acaaggcaaa tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc tctataccat
    30361 gacgcaaacg gacgatttac tgcgcaagct gtacgatcag ttacgcaaca gcggcagcag
    30421 cttctctctg gtttactttt ctgaccacgg tctggccttt aaagagcgcg gcaaagacgt
    30481 gcaatacctt gcccatgatg ataaatatca gcaaaatttc caggtgcctt ttatggtcat
    30541 ttccagcggc gataaagcgc atcgggtgat taaagcccgc cgctcagcca atgacttctt
    30601 aggctttttc tcgcagtgga cgggaattaa agcgaaggaa ataaatatca aatacccgtt
    30661 tatatctgag aagaaagccg ggccgatata catcaccaac ttccagttac agaaggtgga
    30721 ttacaaccat ctcggaaccg atattttcga cccgaaacct taaaaacaaa aatccgcccc
    30781 gagaggcgga tttttatatc accaaagtga ttagaagcgg taaccaacac cggcaatcca
    30841 ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa gagaagtcca gagcaacgtt
    30901 ttccatcggg ttgaactgca gaccagcacc gtaggagaaa ccgtagtcgc tggtgtcgtg
    30961 tttgtaggtc gggtattcag tggtctggaa tttaccataa cccacaccca ctacaccgta
    31021 gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca gtgatgccgt agtactggtt
    31081 tttgttgtag tcaccagagc ttgcagtacg gcttttctcg gtgtaagtga aagaaccgat
    31141 cacacccagc gggctgttgt cttcttcata gcggtatttc aggttgaaac cgcccatttt
    31201 gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca gttacggtag aagtcgcagc
    31261 tacggaagta cctgcggtga aagccagaac tgcggccagt gctgaaagac atgcaatttt
    31321 tttcataacc acctcaaatg tgattcaaat aagtcctaag ttttaaatat atcaaaaatt
    31381 aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca tttcatgtaa cagaacgttt
    31441 ccatacaccg ctatccatct aaatttaaat cactttttca gagaactgcg taagtattac
    31501 gcatgttttc cctgtcattc atccagatta ttcctaatca ccagactaat gattccatca
    31561 atcctggcgc attttagtca aaacggggga aaattttttc aacaaatgct caaccagcat
    31621 tgggtatatc cagtacactc cacgctttac ttaagtctag atatttgtgg gagaaaggat
    31681 gcctggttca ttacgtaaaa tgccggtctg gttaccaata gtcatattgc tcgttgccat
    31741 ggcgtctatt cagggtggag cctcgttagc taagtcactt tttcctctgg tgagcgcacc
    31801 gggtgtcact gcgctgcgtc tggcattagg aacgctgatc ctcatcgcgt tctttaagcc
    31861 atggcgactg cgctttgcca aagagcaacg gttaccgctg ttgttttacg gcgtttcgct
    31921 gggtgggatg aattatcttt tttatctttc tattcagaca gtaccgctgg gtattgcggt
    31981 ggcgctggag ttcaccggac cactggcggt ggcgctgttc tcttctcgtc gcccggtaga
    32041 tttcgtctgg gttgtgctgg cggttcttgg tctgtggttc ctgctaccgc tggggcaaga
    32101 cgtttcccat gtcgatttaa ccggctgtgc gctggcactg ggggccgggg cttgttgggc
    32161 tatttacatt ttaagtgggc aacgcgcagg agcggaacat ggccctgcga cggtagcaat
    32221 tggttcgttg attgcagcgt taattttcgt gccaattgga gcgcttcagg ctggtgaagc
    32281 actctggcac tggtcggtta ttccattggg tctggctgtc gttattctct cgaccgctct
    32341 gccttattcg ctggaaatga ttgccctcac ccgtttgcca acacggacat ttggtacgct
    32401 gatgagcatg gaaccggcgc tggctgccgt ttccgggatg attttcctcg gagaaacact
    32461 gacacccata cagctgctgg cgctcggcgc tatcatcgcc gcttcaatgg ggtctacgct
    32521 gacagtacgc aaagagagca aaataaaaga attagacatt aattaaattt acatttctgt
    32581 atggttatgc ataaccatgc agaatttctc gctacttttc ctctacaccg tatttatata
    32641 tcgaattatg caaaagcata tttattccga aaattcctgg cgagcagata aataagaatt
    32701 gttcttatca atatatctaa ctcattgaat ctttattagt tttgtttttc atgcttgtta
    32761 ccactattag tgtgatagga acagccagaa tagcggaaca catagccggt gctatactta
    32821 gtctcgttaa ttactgggac ataacatcaa gaggatatga aattatgagt accgctaaat
    32881 tagttaaatc aaaagcgacc aatctgcttt atacccgcaa cgatgtctcc gacagcgaga
    32941 aaaaagcaac agtagagttg ctgaatcgcc aggttatcca gtttattgat ctttctttga
    33001 ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt cattgccgta catgaaatgc
    33061 tggatggctt ccgcaccgca ctgatcgatc atctggatac catggcagaa cgtgccgtgc
    33121 agctgggcgg tgtagctctg gggaccactc aagttatcaa cagcaaaacc ccgctgaaaa
    33181 gttacccgct ggacatccac aacgttcagg atcacctgaa agaactggct gaccgttacg
    33241 caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc gaaagatgac gacaccgcag
    33301 atatcctgac cgccgcgtct cgcgacctgg ataaattcct gtggtttatc gagtctaaca
    33361 tcgaataaat ccatcgctga tggtgcagaa ctttagtacc cgataaaagc ggcttcctga
    33421 caggaggccg ttttgttttg cagcccacct caacgcactt atttagtgca tccatctgct
    33481 atccccagct gattaagtaa attttttgta tccacatcat cacacaatcg ttacataaag
    33541 attgtttttt catcaggttt tacgctaaat aatcactgtg ttgagtgcac aattttagcg
    33601 caccagattg gtgccccaga atggtgcatc ttcagggtat tgccctataa atcgtgcatc
    33661 acgtttttgc cgcatctcga aaaatcaatg agttgcaaaa ttggcacgat tttttcatat
    33721 atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa aaggaaatgc tatgaagtct
    33781 gtattaaaag tttcactggc tgcactgacc ctggcttttg cggtttcttc tcatgccgcg
    33841 gataaaaaat tagttgtcgc aacggatacc gccttcgttc cgtttgaatt taaacagggc
    33901 gataaatatg tgggctttga cgttgatctg tgggctgcca tcgctaaaga gctgaagctg
    33961 gattacgaac tgaagccgat ggatttcagt gggatcattc cggcactgca aaccaaaaac
    34021 gtcgatctgg cgctggcggg cattaccatc accgacgagc gtaaaaaagc gatcgatttc
    34081 tctgacggct actacaaaag cggcctgtta gtgatggtga aagctaacaa taacgatgtg
    34141 aaaagcgtga aagatctcga cgggaaagtg gttgctgtga agagcggtac tggctccgtt
    34201 gattacgcga aagcaaacat caaaactaaa gatctgcgtc agttcccgaa catcgataac
    34261 gcctatatgg aactgggcac caaccgcgca gacgccgttc tgcacgatac gccaaacatt
    34321 ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag cggtaggtga ctctctggaa
    34381 gcgcagcaat acggtattgc gttcccgaaa ggtagcgacg agctgcgtga caaagtcaac
    34441 ggcgcgttga aaaccctgcg cgagaacgga acttacaacg aaatctacaa aaaatggttc
    34501 agtactgaac cgaaataata acgctacacc tgtaaagcgc actggcagtt ccctctcccc
    34561 cctggggaga ggattagggt gaggggcgca aacccgctcc ggggccatta attaccctga
    34621 atttgattat ttacaccacg gtaacaggaa caacatatgc agtttgactg gagtgccatc
    34681 tggcctgcca ttccgcttct gattgaaggt gccaaaatga ccctgtggat ttcggtcctc
    34741 ggtctggctg gcggtctggt aatcggattg ctggcaggtt ttgcacgcac cttcggtggt
    34801 tggatagcca accacgtcgc gctggtcttt attgaagtga tccgcggcac acctatcgtc
    34861 gtccaggtga tgtttatcta tttcgccctg ccgatggcgt ttaacgactt acgcatcgac
    34921 ccatttactg cggcggtggt caccatcatg atcaactccg gcgcgtacat tgcggaaatc
    34981 acgcgtggtg cggtgctgtc tatccacaaa ggttttcgtg aagcaggact ggcgctcggt
    35041 ctttcacgtt gggaaaccat tcgctacgtc attttaccgc tggcactgcg tcgtatgctg
    35101 ccgccgctgg gtaaccagtg gatcatcagc attaaagaca cctcgctgtt tattgttatc
    35161 ggcgtggcgg aactgacccg tcaggggcaa gaaattattg ccggtaactt ccgcgccctt
    35221 gagatctgga gcgccgtggc ggtgttctat ctgattatta ccctggtgct gagctttatt
    35281 ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga atttaaaaac gtctccaagc
    35341 actttggccc aacccaggtg ctgcacaata tcgatttgaa cattgcccag ggcgaagtcg
    35401 tggtgattat cgggccgtcc ggttccggta aatcgaccct gctgcgctgc atcaacaaac
    35461 tggaagaaat cacctccggc gatctgattg tcgatggcct gaaggtcaac gatccgaaag
    35521 ttgacgagcg cctgattcgc caggaagcag gtatggtgtt ccagcagttt tacctcttcc
    35581 cgcatctgac ggcgctggga aacgtcatgt ttggtccgct acgcgtgcgt ggcgcgaaca
    35641 aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa agtcggtctg gcagaacgtg
    35701 cacatcacta cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg attgcccgcg
    35761 cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc gacttccgct cttgacccgg
    35821 aactgcgcca tgaagtgctg aaggttatgc aggatctggc tgaagaaggg atgacgatgg
    35881 tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc ttcgcggctg atctttatcg
    35941 acaaaggccg gattgcggaa gatggcaatc cgcaggtgtt gatcaagaac ccgccgagcc
    36001 agcgcttgca ggaatttttg cagcacgtct cttaataaga cacattgcct gatgcgctac
    36061 gcttatcagg cctacaggat atctggcaac ttattaaaat tgcatgaact tgtaggacgg
    36121 ataaggcgtt cacgccgcat ccggcaaaaa agcccgcacg ttgtcagcaa cctgcttaat
    36181 atcccttcct ccctttcacc cgaaagggag gcacaccaga ttcctctcat ttaaaatcgc
    36241 ccctccccca gcatctatac ttatcttttt gctctgtttt ctcactggag gagtcatgcg
    36301 gtggatcctg ttcatcctct tctgcctgct gggcgcacct gcccacgcgg tatccatacc
    36361 cggcgttaca accacaacga caacggactc aacgactgaa ccggccccgg aaccggatat
    36421 cgaacaaaaa aagcggccta tggcgcactg gcggatgtgc tggataacga cacctcgcgt
    36481 aaagagttga tcgaccagtt gcgtactgtc gccgcgacgc cccctgcgga accggtcccg
    36541 aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc tgcaaaaggt caccgaagtc
    36601 agccgccatt atggtgaagc cctttccgcc cgcttcgggc aactttatcg caatatcacc
    36661 ggctccccgc ataagccatt taatccacaa accttcagca atgcgctgag ccatttttca
    36721 atgttagcgg tattagtgtt tggtttttac tggctgattc gcctgtgcgc actgccgcta
    36781 tatcgcaaaa tgggtcagtg ggcgaggcaa aaaaatcgtg agcgcagcaa ctggttgcag
    36841 cttccggcga tgattattgg ggcgtttatt atcgacctgc tgttactggc cctgacatta
    36901 tttgtcggcc aggtattaag cgacaacctg aatgcaggca gtcgcaccat cgctttccaa
    36961 caaagtttgt ttctcaacgc ctttgccctg attgaatttt tcaaagccgt actacgcctg
    37021 attttttgcc caaacgtggc ggagctgcgc ccgttcacga ttcaggacga gagcgcccgt
    37081 tactggggtc gtcgcctgag ctggttaagc agcctgattg gctatggcct gattgtggcc
    37141 gtgccgatta tctctaatca ggtgaatgta cagataggtg cgctggcgaa cgtcatcatt
    37201 atgctgtgca tgaccgtctg ggcgttgtac ctgatcttcc gaaataaaaa agagattacc
    37261 cagcatatgc tcaactttgc ggagcattcg ctggcttttt tcagcctgtt tatccgcgcc
    37321 tttgcgctgg tgtggcactg gctggcaagc gcctatttta tcatgctgtt tttcttttcg
    37381 ttgttcgatc cgggcaacag tctgaaattt atgatgggtg caacggtgcg cagcctggcg
    37441 attattggta tcgcagcgtt tgtttccggt atgttttccc gctggctggc gaaaaccatc
    37501 accctctcgc cacatactca gcgtaactat ccggagctac aaaaacggct gaatggctgg
    37561 ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct gcgtggcagt aatgttgctg
    37621 ttgagcgcat ggggattgtt cgatttctgg aactggctgc aaaacggcgc ggggcagaaa
    37681 accgtagata tcctgatccg catcgcactc attcttttct tctcggcggt tggctggacg
    37741 gtgctcgcca gtttgatcga aaaccggctg gcttcggata ttcatggccg cccgctaccc
    37801 agcgcccgta cgcgtaccct gctgacgctg ttccgtaacg cgctggcggt gattatcagt
    37861 accatcacca tcatgattgt gttgtcggaa atcggcgtca atatcgcgcc attgctggca
    37921 ggtgccgggg cattaggtct ggctatctcg tttggttcgc aaacgctggt aaaagatatt
    37981 atcaccgggg tatttattca gtttgaaaac ggcatgaaca ctggagattt ggtgactatc
    38041 gggccgttga ccggcacagt ggaacggatg tcgattcgct ccgtgggcgt gcgccaggat
    38101 accggggcgt atcacatcat tccgtggtct tcgataacca cctttgctaa cttcgtccgc
    38161 ggtattggtt cggtagtggc aaactacgat gttgatcgcc atgaagatgc tgataaagcc
    38221 aatcaggcac tgaaagatgc ggtaacggaa ttgatggaaa acgaagaaat tcgcgggctg
    38281 attattggtg aaccgaactt tgccgggatt gtcggcttaa gcaataccgc gtttacactg
    38341 cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg tacgctttgc cctcgacagc
    38401 caggtgaaaa aacatttcga cctggcgggc gttcgcgcgc cagtgcagac ttatcaggtg
    38461 ctgcctgctc cgggcgcgac cccggctgaa ccgctgccgc cgggggagcc aacgctttaa
    38521 cgttggcgat taacaaaacg gcggcgctgc tcgtcgttca taaaggtcca ggcaataaag
    38581 cgactctgct tttgcccctg ggccatctct ttttttacca ccttcaccgc gcccacgtcg
    38641 gtcagagcac gatacaacgg cggtaagttt tcaccacgag ataccagcga ggtaaaccac
    38701 atcacctgct tcgcgaagcc tttgctctct tcaatcatct ttttgataaa gttgacttca
    38761 ccgccttcac accacaactc ctgttgctga ccaccaaagt tcagtgcatc gtctttgttc
    38821 agccccaggt tacggcgttt acgctcacta cctgcccgcg ccgcagcggc ggaatcgtgg
    38881 aatggcgggt tacacaaggt cgcgtcgtat tgctcgtttt tgtggatgat gccgttaaaa
    38941 atcgccccac tctctttttg ccgacgcaga cgaatggcgc ggttaagccc cggattagca
    39001 ctgataatcg cctgcgcact gcttaacgcc tggctgctgg tttcactacc ggtaaaacgc
    39061 cagccatatt catgtacgcc aatcagcgga taaatacagt tcgcaccaac gccgatatcc
    39121 agaatgctgg cattcgctgg aattgttccg cttgcttcag ccagtaaatc ggcaaggtga
    39181 tgaatataat ccgcccggcc cggtactggt gggcagagaa aaccgtcggg gatatcccag
    39241 ttcgctacgg cgtaaaaatg ggccagcaac gccttattga gcgccttcac cgccagcgga
    39301 ttggcaaagt ctacgctttg ctcccctgcg ggtgtaagcg tgaggaattg cctgagttca
    39361 ggattgacct gacaaagcgt ggcgagatcg tagcggctgt gatgacggtt gcgcggatgc
    39421 aaccccggtt tctgggcgga catagcattc tcctttaagc agcggcgtaa gatacccgca
    39481 taacgcgaca gggtaaatat ctggctgcgt ttatcattcc ccaaaacctt aacatggcta
    39541 tggtttatgg caattcatca taaaaatcag cattatgcat gtcattgatt tgtcttattt
    39601 tgtaaaaata tatcgttcaa aaaataagca tttctctgtc aattttttgc gtcatttgca
    39661 tcgcgcaggc gacctacact tatcaggcac tacccgttgt atttatgagg atgtcattat
    39721 gaaaaagtgc ctcacactac tgattgccac cgtcctgagc ggaatctctc tcacggctta
    39781 tgccgcgcaa ccgatgagta acctggacag cggtcagcta cggcccgccg gtaccgtttc
    39841 ggcaactggc gcatcaaacc taagcgatct ggaggataaa ctggcagaaa aagcgcgcga
    39901 acaaggcgcg aaaggttatg tcattaattc cgcaggcgga aatgaccaga tgttaggtac
    39961 tgcaaccatc tacaaataac cccaccaacg taactgcatt cgcctctgga tgcagttttc
    40021 gcctgttttg taatcaaaaa acatcaaata tgaacgcaat gtaaataaat gtatttcttt
    40081 ttcgcgcaat gggtgataga aaatcgcatc aagtgataat gcttatcaaa attattatca
    40141 cttgcacgaa cactatcacg ggattaacag tggcatcgca tccgcagaga tgctttctcg
    40201 tggcagtgaa aatttcaaca tataagaaaa agtcacctgc aaaatggaaa acaatcgcaa
    40261 tttccctgcc agacaatttc attcgctcac gttctttgcc ggtctttgta ttggtattac
    40321 gcctgtggca caggcactcg ccgccgaagg gcaagctaac gcggatgaca cgctggttgt
    40381 cgaagcatca acgccttcgc tttatgcgcc acaacaatct gccgatccga aattctcgcg
    40441 tccggtagcg gatactaccc gcacgatgac ggtgatttct gaacaagtga ttaaagatca
    40501 gggcgcaacc aaccttaccg acgcgctcaa aaacgtcccc ggcg
//
LOCUS       DAJTSF010000025        39987 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000025 DAJTSF010000000
VERSION     DAJTSF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.025"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..155
                     /locus_tag="OO335_001427"
     CDS             <1..155
                     /locus_tag="OO335_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6001873.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="OO335_001428"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="OO335_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6001874.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="OO335_001429"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="OO335_001429"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6001875.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="OO335_001430"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="OO335_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="OO335_001431"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="OO335_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6001876.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="OO335_001432"
     CDS             complement(5626..7134)
                     /locus_tag="OO335_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6001877.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO335_001433"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO335_001433"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6001878.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="OO335_001434"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="OO335_001434"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6001879.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="OO335_001435"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="OO335_001435"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6001880.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO335_001436"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO335_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6001881.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO335_001437"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO335_001437"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6001882.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="OO335_001438"
     CDS             complement(14283..15602)
                     /locus_tag="OO335_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR6001883.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO335_001439"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO335_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6001884.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="OO335_001440"
     CDS             16699..17034
                     /locus_tag="OO335_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR6001885.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO335_001441"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO335_001441"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6001886.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="OO335_001442"
     CDS             complement(17790..19172)
                     /locus_tag="OO335_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6001887.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /locus_tag="OO335_001443"
     CDS             complement(19358..20302)
                     /locus_tag="OO335_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6001888.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="OO335_001444"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="OO335_001444"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6001889.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="OO335_001445"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="OO335_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6001890.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO335_001446"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO335_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6001891.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO335_001447"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO335_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6001892.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="OO335_001448"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="OO335_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6001893.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="OO335_001449"
     CDS             complement(25947..26768)
                     /locus_tag="OO335_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6001894.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="OO335_001450"
     CDS             complement(26868..26951)
                     /locus_tag="OO335_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001895.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27379)
                     /locus_tag="OO335_001451"
     CDS             complement(27044..27379)
                     /locus_tag="OO335_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6001896.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27776..29029)
                     /locus_tag="OO335_001452"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="OO335_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30047
                     /locus_tag="OO335_001453"
     CDS             29136..30047
                     /locus_tag="OO335_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6001897.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGL
                     MRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSE
                     MWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30182..31402
                     /gene="mlc"
                     /locus_tag="OO335_001454"
                     /gene_synonym="dgsA"
     CDS             30182..31402
                     /gene="mlc"
                     /locus_tag="OO335_001454"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6001898.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31527..32222
                     /gene="bioD"
                     /locus_tag="OO335_001455"
     CDS             31527..32222
                     /gene="bioD"
                     /locus_tag="OO335_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6001899.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32175..33467)
                     /gene="clcB"
                     /locus_tag="OO335_001456"
     CDS             complement(32175..33467)
                     /gene="clcB"
                     /locus_tag="OO335_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6001900.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33626..34240)
                     /gene="dmsD"
                     /locus_tag="OO335_001457"
     CDS             complement(33626..34240)
                     /gene="dmsD"
                     /locus_tag="OO335_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6001901.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34283..35137)
                     /locus_tag="OO335_001458"
     CDS             complement(34283..35137)
                     /locus_tag="OO335_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6001902.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35139..35756)
                     /gene="dmsB"
                     /locus_tag="OO335_001459"
     CDS             complement(35139..35756)
                     /gene="dmsB"
                     /locus_tag="OO335_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6001903.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35767..37805)
                     /locus_tag="OO335_001460"
                     /pseudo
     CDS             complement(35767..37805)
                     /locus_tag="OO335_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37866..38975)
                     /locus_tag="OO335_001461"
     CDS             complement(37866..38975)
                     /locus_tag="OO335_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6001904.1"
                     /translation="MINVSHIRTLSSIESTTSSPSIRLAMESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38958..>39987
                     /locus_tag="OO335_001462"
     CDS             38958..>39987
                     /locus_tag="OO335_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6001905.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVE"
BASE COUNT        10164 a         9699 c        10241 g         9883 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga
    29761 ctgggctgta tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca
    29821 ctcaggaggt gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt
    29881 caattttgcc tgcgtcattt aaacgtgttc agctcaacga aatgcgctgg gtgccgattg
    29941 ctgaagagga tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc
    30001 cagctgcgcg taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc
    30061 gaaaaagccc gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta
    30121 ttgaagtgct tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc
    30181 ggtggttgct gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc
    30241 ggtttatcgc ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc
    30301 gcagctggct cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt
    30361 gcgagagctg gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt
    30421 tgaaactgaa gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc
    30481 tctgcgcgat ctgagcagca aactggtggt ggaagagtcg caggaactgg cgttaaaaga
    30541 tgacttgcca ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca
    30601 gaaaaaactt gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga
    30661 aaatggtatt gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga
    30721 ggcgctggag cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac
    30781 gatggcagag gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat
    30841 cgatcacaac gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag
    30901 tagtctcgtg gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg
    30961 gaaccacggc tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct
    31021 gcgtctcaat caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt
    31081 gtgtcaggcg gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc
    31141 gcatgtaggg cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat
    31201 tggctcaccg ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg
    31261 tcagcaggcc cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa
    31321 ccagggcacg atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt
    31381 gattcgtctg ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca
    31441 atttgggcca ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt
    31501 tgtgaagcat aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca
    31561 cttctgtagg gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa
    31621 aaagggttgc gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc
    31681 gtaataaaga tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag
    31741 ttaatcctat cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt
    31801 acaccctcat ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag
    31861 aagggactgg cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag
    31921 tgcaggaaca actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg
    31981 cactgctaac agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta
    32041 accgaatcaa cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac
    32101 ttccggcacc gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg
    32161 ggcaatacat ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt
    32221 aacgtccgcg aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga
    32281 tactccccgg tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc
    32341 gccaacagtg tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg
    32401 aaccataatc ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc
    32461 ggtgtaaaga caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta
    32521 caaaggaaga tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg
    32581 gtgctatagc cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac
    32641 ccgcctagtg ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag
    32701 gcgttcatta acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata
    32761 atcagcgcat agtcacgagc ctgaaccgtc actgagagtt gtacgttgta gagtaacgcg
    32821 tcgctatgat taatcagatt gctaaccagc catgccacga tggcggaaat aatcaccggg
    32881 ccgagagagg ccaacatcat agtgccaaac agcacttcgg ctataaataa actgccagca
    32941 agcggggcac gataggccgc agccattccc gccgcggccc cacaggcgat ccataatttc
    33001 cattcctggc gtggcgtaaa acgttgggca aaacaggagg cggcaagggc agctaaaaga
    33061 atcatcgcac cttcgcgacc aattgcactg ccgctggtta ctaccagcag cgaggcaagc
    33121 gatttaacca ggcttgctgc gtaatcgaac tgtccatcgg tttgcaacgc ttccatgtaa
    33181 tcggtcggcg catgagggcg ttgttgggta aatttctgcc agcccatcag caacaaaccc
    33241 gccgccagtc cgccgagcgc cggagttagc agccgtcgcc aggaggaaag gtttgtcgct
    33301 gcattgacca gactgccgga gtcattattg aggaacaacc actccagtag cagcatcgca
    33361 tgacgaaacc cggcaacggc aaagacagcg agaataccga cgactgttgc gataagcaga
    33421 cggcggaaca tagtgcgcag atccggatag atgtgtagat gatgcatgag cagaaatggg
    33481 caatgaaaca cgggcgacta ttttgcggca aaagaggcca accgcaaaag cagtgcgtgc
    33541 tttaagagat aaaaaaacgt gacattgtaa cgtattattg ccgaatcgtg cgtaatcgtc
    33601 accatccggc aatattacgg tgatcctatc gaaagagtgg tttaaccgcg acaggaatca
    33661 acagttgtga ttgccattgc gccagcgtta agcgagccag ttcacccagt gcacggtaga
    33721 aagggtgttc tgctttttcg ataaaaacat caagaaaacg tgttgaccac ggaaaaaggt
    33781 gccatgccag caattcttcg cattctgtct ggcgaccatt ctccgccaac cacgcagcca
    33841 tcaacagcag tgaaccaaaa tgatcttccg gttcgttttg cttcatttca aactgaatgc
    33901 ctttctcgcg catccactga cgaagtgcca atgttgaatc gccaaacagc acagattcgc
    33961 gatccagcca gaccgacccc cacggcggag acggcagtgc ccacgggccg acaaacaaac
    34021 gctgccaggc ctgggcgtga gtctcttcac actgtgtctg aaaagccgtc accagcggcg
    34081 ctaatgacgc ttctggtaga ggccactgag tttcccagcc gtcactggtg agtaccgcaa
    34141 ccagaggtgc ggcttccgca ctctctggag cgtaataaaa cagcgcgccc aacacgcgcg
    34201 ccgcgacaga aaaattatct tgctgtgaaa aatgggtcat tcctaacatc ctgaaatgtg
    34261 cgggttgccc cgcaccaatt tgttaacctg caatagccat tcctactgtc atgtgcaggc
    34321 cataaaagag cacacgcccg atcatctcac caacaagtat gaggattagc cccagaacaa
    34381 gtccggcaac gtgaggttca cggcgacgaa tgagcggaca aagccagcat ccaagacctg
    34441 cgcataacaa taccacacgc cagacctgta aggaggcgta atctggtacc agtgcgctgg
    34501 cttgttgcac ggatgagtga atagacgcca gagacaaacc ttgcagcaca atcacctcgg
    34561 cacaagcgat cagcgccaga acgctaatga tggcaaatgg tgtggtatta aatgtaacgc
    34621 gggctgcccg cagaattgcg gcagcaagta ttggaccgct caacaatacc gtcaggaaga
    34681 aagccagcgt agtgtaaccg ttatgccagg ttggcacggt gtcgatttga tacacgcagg
    34741 tcatcatcca gacgaaaatg acgccgagcg ccatgctgac aagtaaccag agtttcccca
    34801 acgcttgcgg cattttaccg atgaccgcca ccagccacca tagtccgcca acggcaaaaa
    34861 atatggaacc ggcagcaatc tcgttactca gaccagaagc tccgattcga ttcagcgagt
    34921 taaacgctcg cagtggcgat cccaggtgca tgacagaggc aataaacccg atgcccatca
    34981 acagccagag gaaaaacatg ccacgaacaa tgtgctgacg gtctgcgtca tttttggcgg
    35041 cgaaccagcc aattccgcta acaatcagcg cgcctactac gcattgaccc aatacggtaa
    35101 agataaccag tggccattca tgccatccat ttcccatttt acacttcctc cggattagcc
    35161 agataaccag tggtatcgcc ggtcgggcgg ctgttggcgt taggtttgat aacgatattg
    35221 ggttttgtga aatgcgcgcg cggcagtggc gcgacggcag ccagagtgcc gtgcttctgg
    35281 cgcagctctt caattggacc gaactcaagt gcacgcagcg ggcaggattc gacgcatatg
    35341 ggttgtttcc cctcggcgac gcgcgaataa caaccatcgc acttcgtcat gtgccctttt
    35401 tcagcattgt actgtggtgc gccgtacgga caagccatgt ggcagtagcg gcagccgata
    35461 caaacatctt catcaaccac cacaaagcca tcttcacgct tatgcattgc gccgctgggg
    35521 cagacttttg tacatgcggg gtcatcgcaa tggttacagg agatggagag ataataagcg
    35581 aacacgttct ggtgccagat gccattatct tcctgccagt cgccgccagc atattcataa
    35641 atacggcgga aactgacttc cgggccaaga tctttaaaat ctttgcacgc cagttcgcag
    35701 gttttacagc cagtacagcg gctggaatcg ataaaaaatc catattgtgt ggtcatgggc
    35761 tactccttaa accttttcga tctggacaag attgctgtgc gacgggtttc cctttgccag
    35821 cggtgaaggg cggtgagagg tcagaatatt gatactgccg ccgtgatcga cccggtcacc
    35881 aaacatatcc gctttaagcc acgcaccttg cccgatggcg gtaacgccag gcagaatacg
    35941 cggagtcact tttgcggcaa tcagcatttc tccattattg ttaaataccc gcacggtatc
    36001 gccatgacgg ataccgcgtg cctgagcatc aatggggttg atccacacct cttgtgggca
    36061 ggcctgctgt aacacatcaa tattgccgta gctggagtgg gtacgcgctt tggtttggcg
    36121 acttcactca gcatccagta gatgggtttg cgttcaaatt ttgctgaggt tgcgggttgg
    36181 gcgaggatca catagcccat attccctgca gattcatgag agataaggtc ttcttgctct
    36241 gttggcatca ggtcaggcaa caggatatcg caatacttag ccgaggccgt catgaagtgg
    36301 tcaatgccaa caatcatctc gcacttgctg tcatcctgaa gcacctcatg ggtgtgattg
    36361 atgtcgccat gttgattgat caatgtgtta ctggcgtagc accataaaaa cttgatgggg
    36421 acatccagtt tttcttttcc acgaacacca tcacgggtcg cggtcatttt cgtaccatgg
    36481 tcgatggcat ctgtccatgt aaaaacggaa atctgcgttt taacaggatt ctcgagcatc
    36541 gggaaccatt ctacccccag atcccagcta ccttcgcgta cacctgagtt gccgccgttt
    36601 atgccgacgt taccggtaag aacggaaagc atggcaatag cgcgggacgt ttgttcgccg
    36661 ttggagtgtc gttgtggccc ccaaccctga caaatataag caggttttgc tgaaccgatc
    36721 tctcgtgcca actggataat tttttctgcc gggatgctgg tgatttttgc tgcccattcc
    36781 ggcgttttag ctatgccgtc aggcccttcg cccagaatat aggctttata atgcgcgtta
    36841 cgtggtgcgt tggcgggcag cgttttttca tcgtaaccaa cacaatattt gtcgagaaat
    36901 ggctgatcga ccatgttttc agtaatcagt acccaggcaa tcgcacaggc cagtgcgcca
    36961 tcggtgccag ggcgaatggg cagccattca tcttcacgcc cggcagctgt gtcgttatag
    37021 cgtggatcga tgacgatcat gcgtgcgttt gaacgttcgc gggcttgctc gacgtagtaa
    37081 gtgacaccac cgccgctcat ccgcgtttct gccgggttat ttccgaacat aacgaccagt
    37141 ttcgtattgg cgatatcatc cgggctgttg ccatcattgg caccgaacat ataactcatt
    37201 gcggcactga tctgtgcggt actgtagctg ccatagcgac tgagaaaacc cccgcaagag
    37261 ttcatcagac ggtacgggac gttcgagttg gtgatatttc cgccatctac gcctgttccg
    37321 tacaggacat gtacagcctc attgccgtaa tctttcagga tccgccgaag attatcactg
    37381 atggtatcca gggcttcgtc ccaacttatc cgttcaaatt taccttcacc gcgctcgccg
    37441 acgcgcttca tgggatattt caacctgtca ggatgattca tccgtcggcg gatagagcgc
    37501 ccgcgtaaac atgcccgaac ctgatgatta ccgtaaacgt cgtcacctgt cgtatcagac
    37561 tccacccagt acacggtgtc atctttcaca tgcaaacgta gcagacagcg gctcccgcag
    37621 ttaacggtgc aggaactcca gaccgttttc tcttctaccg gagcctctgc cgcccggacc
    37681 atttgggaaa atggcagagt gaaagcactg cttgccagcg cgagactgcc aagtgcggag
    37741 gttttcatca gacttctatg gctgatttca gccttcatga gcgcctctgt ggtatggatt
    37801 ttcatcatta ctcacttatt gcttttcaaa caaaatgtca tgccagaatt tatggttgta
    37861 gtgggttata ttttttcgat ctcgaccaga ttagtgtgct gcgggtttcc cttcgccagt
    37921 ggtgaagggc gcagagtggt tagcgtattc acacatccgc catggtcgat tttatcgcca
    37981 gacatattgg gctcgtgcca ggctccctgg cccatagcgc taactccagg gagaatgcgt
    38041 ggtgttactt tggctggtag ccgaacttcg ccacgatggt taaacacccg caccatatcg
    38101 ccgttggcaa tcccacgttt ctgtgcatct atagggttga tccacacctc ctgacggcag
    38161 gctgccttca ggacatcaat gttgccgtag gtcgagtgag tacgggattt gtaatggaaa
    38221 ccaaacagtt gcagtgggaa ggttctacgt tcaggggagt cccagccttc aaaggttgag
    38281 gcataaactg gcagtgggct tatcacttca tctttttcca gttcccaggt acgggcaatt
    38341 tccgccagct tgctggaata aatttcaatc ttaccggaag gcgttttaag tggatttgcc
    38401 tcggggtcgt cacgaaatgc tttgtaggcg acaaaatggc cattgggatc tttacgctta
    38461 tagataccca tttttttcag ttcgtcgtaa gacggtaacg ccggatcttt ggcaagcatt
    38521 ttggcgtaca gatgttgtaa ccattgttcc tgcgtgcgac cttctgtgaa cttttgatag
    38581 acgtcaggtc caagacgttt cgcgacttca ctcaggatcc agtaaatcgg tttacgttcg
    38641 aatttttcgc tggtgactgg ctggaggaaa atgagatatc ccatgttacc ggcgtagtcg
    38701 ttaggaataa tatcttcctg ctcaacggtc atcaggtctt ccagcagaat gtcggcatat
    38761 tttgccgatg aggtcataaa gttttcgatg accacaatca tttcgcattt cgattcgtcc
    38821 tgcagaattt catgcgtttt gttgatgtca gaatgctgat taacgagggt atttcccgcg
    38881 tagttccaga tggactccat tgccagacgg atggatggac ttgaagtagt tgactcaatg
    38941 ctacttaagg tcctgatatg actgacatta atcatgataa cactgtatat ccatccagtt
    39001 ctttctctgg gtctccctat cggtatcagc aagtctacac ctcaagccct gacacaaggc
    39061 tgacacaaat caatctactc agcgtagcta agcctcagtt agtccgcaga gccaatggtc
    39121 gatatactat ccgcttcaga ctcaagggac agaccacacc gtttctttca gtatcgaccc
    39181 gcagcacaga caggagagta gccacaatgc gtcagagaga gcttgcagcc accgctaagg
    39241 ccttcatgtt ggataggcct gaagtctcac tacaggagct tacagagcat ctccggtcta
    39301 tggctgagca gttcctgaca gacgctagtg acgattactg gaatggactt gaggtcgcta
    39361 ctctggtcga cgagaagtcc aacctgaagg agctagccgc tacgcaagcc ttgagtctgg
    39421 accagcagaa gggcataagg ttagcgctgg aagtcctgac cgcagcacag cagagagtcg
    39481 acactggtga tacctctggt ctgattaagc tgattgatga taataacctt actgatgact
    39541 caactatagg cgactctacg tcaattctga ataatgagca gggggataga ccggcagttt
    39601 tcacacagga gcgccagtca tctgtagttt tttcaagcct agtgtcctcg ctgctggctg
    39661 agaaggtcca gaccctgaag acatccagct acaaggacct ctcgtcctca ctcaatactg
    39721 tctcccggtt cctgcctgaa gacatggacc tgatgtcccg ctctgggtgg ctggctgtca
    39781 gggactctat gctggcttct gaggtgagac catcgacaat caacaagctg ctgaccaaag
    39841 cgaagatgtg tctcgattat ggcctgatga atgggcaact tgagggccgc aaccctattg
    39901 aacggatgaa gctgaccaag gatatcgact ccaagcgcag agcgttcaca gacgaggagc
    39961 tggagagact tctggtccgt gttgagt
//
LOCUS       DAJTSF010000026        39985 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000026 DAJTSF010000000
VERSION     DAJTSF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.026"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(72..1325)
                     /gene="amiC"
                     /locus_tag="OO335_001463"
     CDS             complement(72..1325)
                     /gene="amiC"
                     /locus_tag="OO335_001463"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6001906.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1557..2888
                     /gene="argA"
                     /locus_tag="OO335_001464"
     CDS             1557..2888
                     /gene="argA"
                     /locus_tag="OO335_001464"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6001907.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2950..4776)
                     /gene="recD"
                     /locus_tag="OO335_001465"
     CDS             complement(2950..4776)
                     /gene="recD"
                     /locus_tag="OO335_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6001908.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4776..8318)
                     /gene="recB"
                     /locus_tag="OO335_001466"
     CDS             complement(4776..8318)
                     /gene="recB"
                     /locus_tag="OO335_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6001909.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="OO335_001467"
     CDS             complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="OO335_001467"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6001910.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11375..14743)
                     /gene="recC"
                     /locus_tag="OO335_001468"
     CDS             complement(11375..14743)
                     /gene="recC"
                     /locus_tag="OO335_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6001911.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14756..15106)
                     /locus_tag="OO335_001469"
     CDS             complement(14756..15106)
                     /locus_tag="OO335_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6001912.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(15091..15498)
                     /locus_tag="OO335_001470"
     CDS             complement(15091..15498)
                     /locus_tag="OO335_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6001913.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15495..16058)
                     /locus_tag="OO335_001471"
     CDS             complement(15495..16058)
                     /locus_tag="OO335_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6001914.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16049..16519)
                     /locus_tag="OO335_001472"
                     /pseudo
     CDS             complement(16049..16519)
                     /locus_tag="OO335_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(16703..17497)
                     /gene="thyA"
                     /locus_tag="OO335_001473"
     CDS             complement(16703..17497)
                     /gene="thyA"
                     /locus_tag="OO335_001473"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6001915.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(17504..18379)
                     /gene="lgt"
                     /locus_tag="OO335_001474"
     CDS             complement(17504..18379)
                     /gene="lgt"
                     /locus_tag="OO335_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6001916.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18530..20776)
                     /gene="ptsP"
                     /locus_tag="OO335_001475"
     CDS             complement(18530..20776)
                     /gene="ptsP"
                     /locus_tag="OO335_001475"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6001917.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20789..21319)
                     /gene="rppH"
                     /locus_tag="OO335_001476"
     CDS             complement(20789..21319)
                     /gene="rppH"
                     /locus_tag="OO335_001476"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6001918.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            22004..22693
                     /gene="mutH"
                     /locus_tag="OO335_001477"
     CDS             22004..22693
                     /gene="mutH"
                     /locus_tag="OO335_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6001919.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22762..23475
                     /locus_tag="OO335_001478"
     CDS             22762..23475
                     /locus_tag="OO335_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6001920.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23613..23831
                     /gene="ygdR"
                     /locus_tag="OO335_001479"
     CDS             23613..23831
                     /gene="ygdR"
                     /locus_tag="OO335_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6001921.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23939..24979
                     /locus_tag="OO335_001480"
     CDS             23939..24979
                     /locus_tag="OO335_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6001922.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25012..26202)
                     /gene="lplT"
                     /locus_tag="OO335_001481"
     CDS             complement(25012..26202)
                     /gene="lplT"
                     /locus_tag="OO335_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6001923.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(26195..28354)
                     /gene="aas"
                     /locus_tag="OO335_001482"
     CDS             complement(26195..28354)
                     /gene="aas"
                     /locus_tag="OO335_001482"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6001924.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28940..29971
                     /gene="galR"
                     /locus_tag="OO335_001483"
     CDS             28940..29971
                     /gene="galR"
                     /locus_tag="OO335_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6001925.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29978..31240)
                     /gene="lysA"
                     /locus_tag="OO335_001484"
     CDS             complement(29978..31240)
                     /gene="lysA"
                     /locus_tag="OO335_001484"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6001926.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31362..32297
                     /gene="lysR"
                     /locus_tag="OO335_001485"
     CDS             31362..32297
                     /gene="lysR"
                     /locus_tag="OO335_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6001927.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32284..32976)
                     /gene="ygeA"
                     /locus_tag="OO335_001486"
     CDS             complement(32284..32976)
                     /gene="ygeA"
                     /locus_tag="OO335_001486"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR6001928.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33105..34523)
                     /gene="araE"
                     /locus_tag="OO335_001487"
     CDS             complement(33105..34523)
                     /gene="araE"
                     /locus_tag="OO335_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR6001929.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(34838..35599)
                     /gene="kduD"
                     /locus_tag="OO335_001488"
     CDS             complement(34838..35599)
                     /gene="kduD"
                     /locus_tag="OO335_001488"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6001930.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(35629..36465)
                     /gene="kduI"
                     /locus_tag="OO335_001489"
     CDS             complement(35629..36465)
                     /gene="kduI"
                     /locus_tag="OO335_001489"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6001931.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(36752..37933)
                     /locus_tag="OO335_001490"
     CDS             complement(36752..37933)
                     /locus_tag="OO335_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6001932.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            38188..39417
                     /locus_tag="OO335_001491"
                     /pseudo
     CDS             38188..39417
                     /locus_tag="OO335_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT         9922 a        10812 c         9702 g         9549 t
ORIGIN      
        1 attgaacacc ctaacgggtg tttttttgtt tctgatctcc cataaaaaaa gcgccattca
       61 gcgccttttt atcatcccct tctcgccagc gttgccccat cggcaaaata cgctttaatc
      121 cccgcaagaa tagactccgc aacttcctgc tggaaagtcg ccgttttcag tttacgctct
      181 tcctcaacgt tactgataaa cgccgtttcg accagaatgg agggaatatc tggtgccttt
      241 agtacggcaa acccggcctg ttcaacttga tttttatgca gcttgttgat ttttccgagc
      301 ttattcagta ccgctttacc aaacttaagg ctgtcagcaa tagtcagcga ctgcaccata
      361 tcgaacatgg tgtggtcgac atagcggtca ccgcttttgc tcacgccacc aatcaagtcc
      421 gaggcgttct gggtttgtgc cagatatttt gccgcagtac tggttgcgcc tttggttgag
      481 agcgcaaaca ccgaggaacc gctcggctga cgactggtaa aggcgtcggc atggatagag
      541 acaaacaggt cagcacgctg tttctgggct tttgctacgc gcacttgcaa cggaatgaag
      601 atgtcttcat tgcgcgtcat gtacaccttc atattgccct cttcctcgat cagagagcgc
      661 aaacggcgag ctatttgcaa taccacatct ttttcgcgcg ttttgtattt ccccaccgca
      721 ccggagtctt cgccaccgtg gccagggtca agcataatga caatcggacg atcgcgccct
      781 gctttacccg gttgtggacc actttgcgct ggcggcacct gcttttcgag gtcgcctttg
      841 ttgtaatcct ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt ggccggatag
      901 aggtccatca ccagacgctc tttaaacccg gcgaccggcg caagggcaaa cagctgcggt
      961 tttacgtttt gctttaattc aaaaaccatg cgtacggttt gcgggtcaaa ttgcccgacg
     1021 cgcgccgact tgatgaacgg gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc
     1081 gagttcaggt ttacatcttc gatatccacc accacgcgtt cagggttact caacgcgaac
     1141 tgcttatatt tcagctgacg atttgattct accgtcacgc gggtgtagct ggacgcaggc
     1201 cagacgcgca ccgccacgac ctggctaacc gcagccaggc tgacctgact tacgctcaat
     1261 agccacatag cgcccgcgcc ttgcagtaaa cgacgacggc tgattgcagt gttggatcct
     1321 gacatgcctc tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg actaatttga
     1381 ccggaaactt tagcgaaaga cgcataatct gtcatctaat aaacggtaaa cattcttttt
     1441 atattcacgg cattactgat aaaaaagtcg ctctcgcata aaatttacac ttgcaccctg
     1501 cgaaaaaaca gaataaaaat acaataattt caaataatca tgcaaagggg tgtgccgtgg
     1561 taaaggaacg taaaaccgag ctggtcgagg gattccgcca ttcggttccc tatatcaata
     1621 cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt
     1681 tctccagtat cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg
     1741 tctatggcgc acgtccgcag atcgacgcaa atctggctgc gcatcaccac gaaccgctgt
     1801 atcacaagaa tatacgtgtg accgacgcca aaacactgga actggtgaag caggctgcgg
     1861 gaacattgca actggatatt actgctcgcc tgtcaatgag tctcaataac acgccgctgc
     1921 agggcgcgca tatcaacgtc gttagtggca attttattat tgcccagccg ctgggcgtcg
     1981 atgacggcgt ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgctcc
     2041 atcgtcaact ggaaagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg
     2101 gcgagagctt taatctgacc tcggaagaga ttgccactca actagccatc aaactgaaag
     2161 ctgaaaagat gattggtttt tgctcttccc agggcgttac taatgacgac ggtgatattg
     2221 tctccgaact tttccctaac gaagcgcaag cacgggtaga agcccaggaa gagaaaggcg
     2281 attacaactc cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg
     2341 tgcgccgctg tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct
     2401 cacgcgacgg tatcggtacg cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa
     2461 caatcaacga tattggcggt attctggagt tgattcgccc gctggagcag caaggtattc
     2521 tggtgcgccg ttctcgcgag cagctggaga tggaaatcga caaattcacc attattcagc
     2581 gcgataacac gactattgcc tgcgccgcgc tctatccgtt cccggaagag aagattgggg
     2641 aaatggcctg tgtggcagtt cacccggatt accgcagttc atcacggggc gaggttctgc
     2701 tggaacgcat tgccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca
     2761 cgcgcagtat tcactggttc caggaacgtg gatttacccc ggtggatatt gatttactgc
     2821 ccgagagcaa aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt
     2881 tagggtaagc gatgaaaatc accggatgcg gcaggcgtaa cactcgtgcg tcgcatccgg
     2941 caattacgtt tattcccgcg aactaaacaa cgccgccaga ccactgcgcc gctcagtacg
     3001 agtggcgatt gccgcactta atatacgctc atcggcatac agcgacagac gacgacgcgc
     3061 gcgggtcacc gcggtgtata ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa
     3121 aatcaacgcc gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt
     3181 agtttcgtgc tctggcaagc gactcggttg cacagactta atattgccgt ccggcatcgc
     3241 aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa
     3301 cccaagcgcg ctgtcattac gggcaatcat caccggtcgg ccttcgtacc aacgagagtg
     3361 cggatgacga tgaattttgc gcttctgttg cataaactgt tcaattcgct cattcagtcc
     3421 agccacgcca aacggccctt cccgcagggc gcacaaaagc tggtactcat tgaacgcctg
     3481 aatgattaaa tccggctcgg cacgcgcttg cagcagatcc agataacgtc cgtaacccgc
     3541 aagagcttcc tcaagcatcg caatataatc ttcgccgctc tgtaaaagtc gtttttcgat
     3601 atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc
     3661 cgcagctaac tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca
     3721 gagactgtcg cgcaaagatg ccgcttctgt gccggttcct gccggaacgt gagtccccgt
     3781 caggcggctt agctgcctgg cacgctcggc ggtaaagccc gcgttggcat aagcgcagat
     3841 atcgcccagc acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat
     3901 cactcgcgca tgatctggca aggcgtcgat cagtctcgac atcataggca gatcgatcat
     3961 tgacgcttca tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa
     4021 acgctggcta cccggctgcg cgcccagcaa tcggtgcaaa gtgctggcat cttccggaat
     4081 gcgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt
     4141 taagcgcgcg gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc
     4201 ggccatttga attaacgctg ccagcaactt cgccacggtg gtcgttttac cggtgcctgg
     4261 gccgccggaa atcaccgaga tccgccgcgt cagcgccact gccgctgcaa ctttttgcca
     4321 gttaatttca tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc
     4381 aacctcaatg gtatgattca cctcattgaa gaagcgcgct accgtccgct cgttacacca
     4441 catgcgattc aaataaagac gatcgccaca gaggatcatc ggcgtgggtt catctcccct
     4501 gctgaccgct tgagaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact
     4561 gacacaggtc gccaacagcg gattcgacgc ctcgttattt tccagtcgtg aaagcggcaa
     4621 acagacgtgt ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacggcagg
     4681 atgttcatct cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg
     4741 ctccacagct tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg
     4801 gcaaacatct catccatcag ggcaatcaac ccggcgttgg gtcgggttgt gtaaatccct
     4861 tgttgcggat gttctttatc aacgccacgc aggaacagat agataacgcc gccaaagtga
     4921 agatcatagt cgtaatcagc aatgcgatgg cgcagataac gatgcagcgc cagggtataa
     4981 agctgatatt gcagatcata gcggtgtgcc tgcatcgctg ctgccatagc ctgttgggtg
     5041 taagccgaac tgtcttcacc cagccagttg gatttatagt cgagcaggta ataacgccct
     5101 tcgtggcgga ataccaggtc gataaagcct tttaacatgc cgcgtacctg catgaactcc
     5161 agcggcgggc agccagcgga tagcgggtca aactggcgaa ttagtgcatc aagctggctg
     5221 gcgatgagcg gttcgctaat cggcaggtaa aactccatct ccacctgttt atcgcggtcg
     5281 gaaagctgac tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc
     5341 cactcggtca acaccggttc ccactgcgat tcaaagccgc cgagttccag tttttcctgt
     5401 acccagttcg ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat
     5461 gtccccggtg acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg
     5521 ctgaccacgc ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga
     5581 ccacgctgct gcaagccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt
     5641 agtgtcctcg cattcagttc tgcagttaaa gcatcattaa cctgccaggg ctggttatca
     5701 ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga
     5761 agccctgccg catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg
     5821 acgtcggtgt cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg
     5881 caatgccaaa ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt
     5941 tcgacctccg cgaggtcgac gctttctggc gcagcattaa gatccaaaac tgcctcaaac
     6001 gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga aattggtaat aaatggcaac
     6061 cagaccagcg gatattccag ccctttcgat ttatggatcg tgacaatctg caccagatgt
     6121 ttatcacttt cgaggcgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt
     6181 tgcgataacc agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgaagc
     6241 agttcgctga tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag
     6301 ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg
     6361 cgccagattt gccgataacc atcgaactct tcgactaccg catcccacgc atgttcgtca
     6421 ttattcagcg tttcaatatc cagcgcattc agccccatca ttgacgttgc cagcgcactg
     6481 cgcagggtgt tctcacgttc gggcgtcatc accgcctgca acaaccaaag catttcctgc
     6541 gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc
     6601 aacgttaagg catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacgctgatg
     6661 tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt
     6721 ccggcttgta gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgatag
     6781 tcgccaacgc cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca
     6841 cctttaaata caaaacgtaa cgcctgattt ttcccggctg atttcactgg aataaacggt
     6901 atttcgcgaa acatgaacgt gtcatcagtc tggctgaaaa gcttgttcac actgttcacc
     6961 attccaggtg cggagcgcca gttggtgtct aaagtgtagt gggcatgaac ttcactacgc
     7021 gccttcatat aagtgaagat atccgcaccc cggaatgcat atatggcctg cttcgggtcg
     7081 ccaatcagca acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac
     7141 tgctgggggt cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc
     7201 gccgctgcca acacctcgcc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg
     7261 tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca
     7321 gccaatgcgc gggtgatcaa cagatcgcgg atcgacaatg gttctgcaag cagttgatcg
     7381 atcgcctcaa acagtggatg tcgcggggtt tcccccccgg ccttcgtgcg atcttctaag
     7441 aaacgctggg agaatttttc cagcgactcc ggcaactgat aactgtttgt ctcttcttct
     7501 gcccaggcgc tgatcttttc gatccattta gcctgattgc tacggttaaa cttgcgtcga
     7561 tcaataccag aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt
     7621 tttaccgtat caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg
     7681 ggcggcggtg ctttgataac cggcggttcg ccttgcagat aacgattaat atcgcgcagc
     7741 aacgcctgcg gccctttcca ggtttcaaag acgacctggg ctatttcacg cggcagcggg
     7801 tagcagtggc gacgccagaa atcggcgcag gcctggtagc gtagcagaga ctcatcttca
     7861 atcagctgct gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg
     7921 caaaagccgt gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac
     7981 cactgcgcgg cttgcgcttt atcgtcgatc tcttccagca ggcgttcgta cagtggattg
     8041 tcggtggttt cacgcagaca ggcgatgcgc aactcgtgga tattgctacg gatacgaccg
     8101 cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacgatcagc
     8161 gggcggggaa aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca
     8221 atcgtaaagg ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc
     8281 aagcgcaaag gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat
     8341 tgtttgctgc aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc
     8401 ggctttcccg ttctggctgc cggaaatctg cgacagaata gccatgcctt gcggctcgac
     8461 caccgcctga tggaagaaat cagcaagttt ttgcggcgtc agcagtttta tctgggccac
     8521 gattttatca cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga
     8581 tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat
     8641 ttgcgcaaac tcatctggct tcatcgctcg caattttgcc tctgcggttg ggaaaaacgc
     8701 cttgtaacgc tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat
     8761 gccccactga cgccccacgc tcattggaaa cgcaaacacg gcatagccca attgttcttc
     8821 ggtacgcaac tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc
     8881 tgagctggtg tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt
     8941 gctgttaccg gctttttcaa agatgacgga ttgtttttta tcgaccacta catctttgtt
     9001 tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt
     9061 tgcctgggcc tcggtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa
     9121 ggcgtcgcga taggccagca cctctttcaa cgtaatggag ggcaaaattt tacgccgttc
     9181 atctcgcgag aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcaaa
     9241 cgctttaccc ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag
     9301 ctgctcttcc gtagcggtat agctaaagta cccctcgagc aatgcctgga acagctgcgg
     9361 cagacgctgg gtgtaaccat tagcattaat cataaggccg ttgttagcgt tggtggaaaa
     9421 acttatgcca ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata
     9481 atcattgagc gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa
     9541 aatcaggctg acatcagctt tgggctcgct ggcaaaataa cggcttggcg catacaccac
     9601 gcgcagattc gactcatcaa caatcagctc tggatggtcg tatttcttct ctgacttaat
     9661 cagcgagaaa tcatcaggaa tataagggtt aagctctggc aaagagagcg caatgtcggc
     9721 ggcttttttc tgccagtcgg cgaaagtttg tgcgctgatt ttatcgacct gatacggcgc
     9781 atcgacaaag taagccgttt tgttgtgcgg ctctttcggg ctgatatacc agatacgcgc
     9841 attctgcggc gtcatcatcg ccagacgttc ctttactgct ttagcatcgt accgatcggc
     9901 aatattgact gcatccagcg tatgctcaac aggaacgcga atcatggtat ctgccagcca
     9961 ttcgacgtaa tccatatcac gggtgatcga cggataacgg aagtcgatat ccagcacatt
    10021 cgccagttca tcgaagtatt gtttatcaat gcctttttca cgtaacagat tgagatagct
    10081 aaaaattgcc gccacaacct gatcgcgatt agccaggcct ttatcagtta aagacgcaga
    10141 gatcgctaat acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc
    10201 aactaatccc tgcttttgca gccagtcaga aagtgtacct gggctgcgat tgccaatcag
    10261 ataggtaatc aattcatcgg ttttactacg gaacttcgct gagttgttat cgatgcgaaa
    10321 ctcaacgcgc aacactttac gcggcagcgc agggacgtaa tgaatgataa tgcccttttg
    10381 cgcgtcggtg actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg
    10441 accaaaggtg tccgccgcca tttttgccaa ctccggcagc ggtttattac tgtaaataac
    10501 cgccttcatc aaattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac
    10561 cggattacca ggtttgtcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg
    10621 gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg
    10681 cgccatggtt aattcagcgt tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag
    10741 caagggttct gcaatagcat cggccaggcg gtctaccgca ccgggcaagg cgtcgttctc
    10801 aacttccaga tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg
    10861 cattttgaga tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact
    10921 catatgttca aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac
    10981 gggcactacc agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac
    11041 cataccgtta tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat
    11101 ggtttcctga atcggctgcc atcccgtttc tgcctgactt aatggtgccc agagggcaac
    11161 aaacaacaat aatgctttga accaggtgct gcggggcatt cacggacctc ataatcaact
    11221 ttattttctg gacagattca acaggttaac gcaaatcatc tgccagaatt taatcttgtg
    11281 ctgcacgagt cagcctatgt ttatataacc atcagtccgt gactggtgcg catcataaag
    11341 taagcagata gattgcgcaa tttttataca acactcatga ctgattaaag cgaaacagcg
    11401 gtaacaggaa acgttgcgac tgttcaacga tggcctccat tgtctctggt gttaattgcc
    11461 gccagagccg ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt
    11521 aagcctgaag gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat
    11581 cgttttgcgc gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca
    11641 atggcgcgga cattccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct
    11701 gttcggctgc aagcggcgga aaacgccact caccgtcttt gcgtagaaaa aggcgacttt
    11761 cgccattacc accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg
    11821 ccacacttaa taaagaggga cgccagcgca acaggccatc cggctgcacc tgcggcaacc
    11881 agccagttat ctgcacaccg ttgcaggcga gatcaatttc catactctgc cccggctggc
    11941 gacaggcaat gactctgtcg gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa
    12001 tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt
    12061 cggcatcatc ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa
    12121 gtccttccag aataaatggc tcggtgtctg ggatttcgct gtcttcagta tggaagttca
    12181 cctgcaaacg catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt
    12241 ccagcggcac ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt
    12301 taccagcctg gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct
    12361 ggtagttttg cggatcaaac ggcatccggg tatggaggca agtaagatgc gcttttaccc
    12421 ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga
    12481 tgtagtcgat cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg
    12541 aacgcccgat atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca
    12601 ggtagcggtc gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt
    12661 caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa
    12721 ccactttgaa cggaattgaa cgcattggca tcagagtaca aatgttaacc ggtccggcga
    12781 gaaaacgctg gctgatacgt tcttgatcca gacgctgtgc cagttcatca cgcaatagtg
    12841 acagcggcac cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt
    12901 gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgttga
    12961 gcatatcgcg acaaaccggc aaccactctt ctagcggacg ctcctgtgcc agcccgcgac
    13021 gccagatgtt tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc
    13081 cgctcgattc atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt
    13141 agcccagcaa catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt
    13201 ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccact
    13261 ggcgtaaata acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat
    13321 ccagcagcgc cagcacatcc tctgacacaa agcgactgtc aggcagtgat aacaggctga
    13381 taaacgcttc cagtaccgga tgtgactgcc gcgcacgacg gtcggaaatg gcgtaaggca
    13441 ggtaacgatc cgcaggtgcg ctaccaaaca ctgcctgaat aaacggactg tagctgtcga
    13501 tatcagccac catcacgatg atgtcgcgcg gagtaagtgt cggggcttcc tccagcatcg
    13561 ccagcaagcg atcgtgtaaa acttcaactt cacgctgcgg gctatggcaa acgtggaatg
    13621 tgatactgct atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga
    13681 tgttcacacc cgcaacggcg cggttttcca gttccagaat gtcagactga atgttatgca
    13741 gcaggttatc tggcgtcaca tcgacaaaag cgtccagctc ctggctgctc tccaggtcag
    13801 aaaggagata aatgtagtcg cgcccaagct tcccccatga ggccagcagc gagttgccga
    13861 catcctgttc accatcgctg ttaaagagct gtccggcatt ttcgctatcg cgaaatagcg
    13921 gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgagt cagcagtttc gccagataag
    13981 caggatcttt aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt
    14041 caatatgttt acccagcgcc tggagcgcct ggagataaac aggcggtaac gcggaaatac
    14101 cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt
    14161 cgataaagcg ctgatagaga ttcgcgcggt gccagcgcgg ttgtccgagt tcatgggtat
    14221 attccaccag cgccttccac aacggggctt gccaggcctg tgcttctccc agcccttcaa
    14281 ccaaatgtcc tgtttcccac tgtgccagcc agtccggacg atagaccaga tactggtcaa
    14341 acaggtccgc cgcttttgag gaaagctgga acagttttcg cttgtcgcta tcgtcagtca
    14401 gataatgccg caacagggta aagtcttcgc gctccagcaa ttgcggcagc agagtcatca
    14461 gtttccagct catgctctgt ttgttaaagg cgctctcttt ggggatttcc ggtaacaccc
    14521 ggacgaacat atcccagata aagctcgctg gcagcggaaa atcaatgttt gccgcaatac
    14581 caaacttttg cgacagggtc atttgcagcc actgcgccat accggtactt tgcaccagaa
    14641 tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg
    14701 cttccagcac gtccagacga ttggaatggt agacccttaa catagcggct cctgactact
    14761 gacaattcgg gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg
    14821 taacgctgat gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttgg
    14881 caggtggcga aatcgcgcgc agttgcgttt gctgccaacc atgccgccag agcttttggt
    14941 actggtttct gctggcgaga ctgttcattg atgttcgctg gatacccgat aacgcagtga
    15001 caatcatcac catcagcacc atcagcacca tcagcaccat cagcaccatc gcaaccatta
    15061 cctccggcag gctaaagcct tgttgattct tcagggaagc tgacataatg ccccctcttt
    15121 cagcggacaa aaatcgctcc agccgcgtgg cgaaaaaaca atttttccat caatgacttc
    15181 gcctgttcgc cacaacgaaa cgccttcata accggcaatc aataaggctt cattatctgc
    15241 cagtaaacgc aaacaaacct gggcatcggt tccagcgtac tgcgagcact gaactgctgg
    15301 ctgcgtctgc caggagtgca tttttcccca cgccagcgcc gactgaacga tggcctggcg
    15361 gcgcaatgac tgactttcca tgctcacgcg agaggcaaaa ctgcgatcct gctgactcat
    15421 tccttgcaat agcaagctac ccaaaaccag cagcatcagg accagagcca gtgacgaaac
    15481 acctttttcg cggttcacag gttgaatcct gtcacgctat agctggcatc caccacggtt
    15541 tgcggttcag acttactggc agcacgcata ttaaccgtca acaccggcga gaagccgctg
    15601 acatcctgac gtacgacctg aaaagtgtcg ataatgatgg catccggatt agtgacttta
    15661 tcccagccct taccttcaca ggatgtcgca ccgcgtagcg tttccagcac atgctccttc
    15721 agacgaaatc caatctggtc ggactctttt accggttcgc gatcccagat accgttactg
    15781 ttcgcatccc actgcacaat gatacagtca ccctgtccga caatttccag cccttcgccg
    15841 gtacagctgc catgacaata acccgccctc tggagatgct tcgcgacggt aaatacccgc
    15901 agccagattt catcttccag tgccagctta cgagtgctcg ttaaactttc acgctgtaac
    15961 gcaggcagaa agcgtgcagc ccccagcaac aatacgctac tgatcgccat agcaatcaac
    16021 acttccagca gagaaaaacc ttgctctttt acaggcatcc ttctgtttct ccttgctgat
    16081 aaagccggag tcttccccac ggcgaaacca ccagccacca ctcgcccgtt gagtttttga
    16141 agcgaatatg cacggcccat gcggtattgc gcaggccaaa gaaagcaagc gaaggtgtca
    16201 ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac caatggtgaa ctgccatgac
    16261 aggtattggc cccagcagcg gaactcacaa ggcaccataa cgtcccctcc ctgataacgc
    16321 tgatactgtg gtcgcggtta tgccagttgg catcttcacg taaatagagc aaatagtccc
    16381 gcgcctagct ggcggtttgc caaagccgtt gcgactgctg ccagtattgc cagccataga
    16441 gtccacttgc gcttagcatg accaaaatca gcatcgcgac cagcgtttca atcagcgtat
    16501 aaccacgttg tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga cgagggccag
    16561 tttctatttc ttcggcgcat cttccggact atttacgccg ttgcaggacg ttgcaaaatt
    16621 tcgggaaggc gtctcgaaga atttaacgga gggtaaaaaa accgacgcac actggcgtcg
    16681 gctctggcag gatgtttcgt aattagatag ccaccggcgc tttaacgccc ggatgtggat
    16741 cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt
    16801 tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca
    16861 tatggttgct gtacaggtgc gtatcgccac cggtccagac aaaatcaccc acttccagat
    16921 cgcactgctg cgccatcata tgcaccagta acgcgtagct ggcaatgttg aacggcaggc
    16981 cgaggaagac gtcacaggag cgctgataaa gctggcaaga gagtttgccg tctgccacat
    17041 agaactggaa gaatgcatgg cacggtgcca gcgccatttt atccagttcg cctacgttcc
    17101 acgctgaaac aataatgcgg cgcgaatccg ggtcgttttt cagctggttc agtaccgtag
    17161 taatctggtc aatatgacga ccatctggcg ttggccaggc gcgccactgt ttaccataca
    17221 ctggcccgag gtcgccattt tcatcggccc attcgtccca gatggtgaca ttgttttcgt
    17281 gtagataagc aatgttagta tcgccctgca ggaaccacag cagttcgtgg atgatggaac
    17341 gcaggtggca acgtttggtt gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct
    17401 gatgaccaaa aatggaaagc gttccggttc cggtacggtc gtttttctgt gtgccttcgt
    17461 cgagcacttt ttgcatcagt tctaaatact gtttcatggt tcctcaggaa acgtgttgct
    17521 gtgggctgcg acgatatgcc cagaccatca tgatcacacc cgcgacaatc atcgggatgg
    17581 aaagaatttg ccccatgctg atgtactgca cccaggcacc ggtaaactgc gcgtcgggct
    17641 ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc aatcaggaac aagcctgaga
    17701 cagctcccat tgggcgcggt ttacgaatat acaggttgag gataataaac agcaccacac
    17761 cttccagcag cagttcgtaa agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga
    17821 aaatggattg ccactgcggg ttggtttgca gcagcaaaat atcttctgta cgggagcctg
    17881 ggaacagcat ggcaaacggg aagttcgggt caacgcggcc ccacaattca ccgttaataa
    17941 agttgcccag acgcccggca ccaagaccaa acggaatgag tggtgcgata aaatcagaga
    18001 cctggaagaa ggaacgttta gtacggcggg cgaagataat catcaccacg ataacgccaa
    18061 tcaggccgcc gtggaaagac atgccgccgt cccagacacg gaacagatac agcggatcgg
    18121 ccataaactg cgggaaattg tagaacagaa cataaccaat acgtcccccg aggaagacgc
    18181 cgaggaagcc cgcatagagt aagttttcaa cttcattttt ggtccagcca ctgcccggac
    18241 gattcgcccg acgtgttgcc agccacattg caaaaatgaa ccccaccaga tacatcaggc
    18301 cgtaccagtg aagcgccacg ggtcctattg agaaaatgac cggatcaaac tccggaaaat
    18361 gcagatagct actggtcatc tgtcaccaca agttcttgtt atttcgctga aagagaacag
    18421 cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg tgcgaataat agcacaaggg
    18481 gacctggctg gttgccggat accgttaaaa gatatgtata tgatccgcgc tataaccctc
    18541 cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc tgcaacctga tgacgaactt
    18601 cggtcgccag ttgcgcttcc agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa
    18661 tgcgccgcag caggtatttt acccgcgcta cagaacgtcc gttcatagac agatggcgat
    18721 acccaagccc aatgaggatt gccacgcaca tgggatcgcc cgccatttca ccgcacaaac
    18781 gaagatcgat accatgtatt tccgcttccc gggcgatcat cgccagagct cgtaacattg
    18841 caggatgaag actgtcgtaa atgttcgcca cccgggtatt gttgcgatca acggccagaa
    18901 tgtattgagt cagatcgttg gtgccaacag agatgaaatc gaccctcttt gccagatgcg
    18961 gcagcataaa taccattgac ggcacttcca gcatgatgcc gatacgtggt ttgggaattt
    19021 cgtaaccgat catctcctcg acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt
    19081 caacttcatc gaggctggtg accatcggca acagaatatt caggttgccc gtagcggcat
    19141 tagcacgcag catcgcccgc acctggatca agaagatctc cggctgatcg agcgtaatgc
    19201 gaatcccacg ccaacccagg catggattct cttcgctgat cggcatgtaa ggcagctgct
    19261 tatctgctcc gacatccagc gtacgcaagg tgacgggttt atcattgaac atttgcagca
    19321 tcccctgata ctgcgccacc tgttcttctt ccgacgggaa accactttgc agcatgaatg
    19381 ggatttcagt gcgataaaga ccgatgccat caatacagct gcccagtttt tcttcatgtt
    19441 ccgggcttaa accagcattg agcatgactt ttatacgctc accgcttttt aactgcgcgg
    19501 gtaaattgac gtcatcttcc gccagacggc taagctcaat ctcttcacta attagccgct
    19561 gatattcttg cagcagtacc ggctccggat cgaccagcaa ttcaccgcga tagccatcaa
    19621 cgatcagcgt cctacgatgc agcaccgaag gctgaatatc cgcgcccatc acggtaggga
    19681 tccccagcgc acgtaccatg atcgcagcat gggagttggc tgcgccatct cgcacgacaa
    19741 caccgactaa gcgatcctgg ggcagctcag caagagttgt cgctgacagt tcatctgcca
    19801 ccagaatgaa acgttccggc caggcgttcg gcccttgatt agcgtcatca agatgaaaca
    19861 gcaatcgctg acccagcgca cgtaaatcac cagcccgctc tttgagatag ttatcgctta
    19921 gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt taccgcccac tctgccaccg
    19981 agcctttatc aacctcggca aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg
    20041 agtaaagatc gaaaatagcc gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt
    20101 agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt cagtcgttcg cgttccagag
    20161 ccggatccag cgttgatgcc tgatacacct gttccattaa aggtaacgtg gcatcctgcc
    20221 agccttcggc aatcgccaca ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat
    20281 attgcccaaa cagggcagtc aactgcgact gagaaagaat agctgccatc tgggtggcaa
    20341 gcgtcaccag gaaggattct tcactttcgt catactggcg caactctcgt tgctgtacca
    20401 ccagtacacc aagcaactgg cgacgttgaa taattggtac gcctaaaaac gcgcggaaac
    20461 gttcttcttt tacggagggg atgtatttga agctggggtg cttttgcgca tctgcaaggt
    20521 ttatcggttc cgccagcctg ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta
    20581 cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat caggtagtaa caacgtcgat
    20641 catgatcggc caggtagacc gaacagacct cggtatccat cgcaagacag atgtcggtaa
    20701 ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc taccttttcg actatttcgc
    20761 gcaggcgagt gagcataatt ggcgtgactt aacctctttt acgtcgataa gcagatgcgt
    20821 tttgtggttt tggcgtattt tcctgcagtg acatcaccac actcgcgaac tctttcatta
    20881 ccctacggta gacatcacgt ttaaatgaca ccacctgtct gaccggatac cagtaactta
    20941 cccatcgcca gccgtcaaac tctggtgtac tgctggtttg catattgatt tctgcatcgc
    21001 cgcttaccag ctgcaagaga aaccattttt gtttttggcc gatacaaacc ggcttcgtgt
    21061 cccaacgcac caaacgtttc ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga
    21121 ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa ttcacggtac atcgcctgct
    21181 ctgcggattc tccggggttg attccgcctt gcggaaattg ccaggagtgc tgaccaaatc
    21241 gccgggccca cattacctgc ccctggcgat tacaaatcac gatacctacg tttgggcggt
    21301 agccatcgtc atcaatcacc ggactacctc aaaataaagc tttatatacg aatgattgtt
    21361 tcatactcca ggaagacggt aaaccactct ctgcagggca ttacacacta ataacaattg
    21421 aataactcac agttatgtgc agagttataa acagaggaag gggtggatag ccgtttttat
    21481 tcaccttttc tgtggataga gttgtgaaga actacggaat tactacggga aaacccggag
    21541 cattctgaat aagcccgata tagacatttt aaaatattca ttattagtca atgcattaca
    21601 tgtaatttga gtaatgaaaa agagtaattc gtgacccagg tcacacctct catttacggg
    21661 ttggcgaaag atcaaccaat gccgtgttta tccacagaat gtgccactaa gttaagcact
    21721 gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg taaatgaaaa cagtagtgga
    21781 ggtttttcac agttatccca gctttctgtg gataacatgg tgtaagatcc tgtttatttt
    21841 cagtgaccag atttggaaaa cctattgcag tgttgcgcaa ctcgattacc ggcagcctaa
    21901 aaagcaatat aaatcagaga attgaacaac acatgtggaa aaagttacac tgcgaatatt
    21961 cggcacataa ttgctgtttg ttttttaacc aaggtatcat gacatgtccc aacctcgccc
    22021 actgctctct cctcccgaaa ctgaagaaca gttgttagcg caagcacagc aactttctgg
    22081 ttatacattg ggagaactgg cggcacttga cgggctggtt acgccagaga atttaaaacg
    22141 cgataagggc tggattggcg tgttactgga gatctggcta ggtgccagcg cagggagtaa
    22201 acctgagcaa gattttgctg ctctgggcgt ggaacttaaa actatccctg tggatagtct
    22261 tggtcgtccg ctggaaacaa cattcgtttg tgttgcccgg ttaacgggta atagcggggt
    22321 gacctgggaa accagccatg tgcgccacaa gctcaaacgc gtactgtgga taccggttga
    22381 aggcgagcgc agcatcccgc tggcgcagcg tcgcgtagga tcaccgttgc tgtggagccc
    22441 gaatgaagag gaagaccgac agctacgcga agactgggaa gaattaatgg atatgattgt
    22501 tctcggtcag gttgagcgga tcaccgctcg tcacggggaa tatttacaga taagaccgaa
    22561 agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc cggggcgaac ggattctgac
    22621 gctgccacgc ggcttttatt tgaagaagaa tttcaccagt gcactactgg cccgtcattt
    22681 tctgatccag tagccatcgc tttgacctgc cgctttccgg gcatataatt accgcttcat
    22741 ttttttggca gggcttttta gatgttattt gcatggataa ccgatcctaa cgcttggctt
    22801 gcgctcggta cgctgacgct gctggagatc gttcttggga tcgacaatat tattttcctt
    22861 tctctggtgg tggcaaagct tcccacagca caacgtgctc atgcgcgccg tctggggttg
    22921 gcgggagcca tggttatgcg tctggcgctg ctggcatcaa tctcttgggt tacgcgcctg
    22981 acaaatccgc tttttacaat attcagtcag gaaatttctg cccgtgattt gattctgctt
    23041 ctgggtggct tgttccttat ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt
    23101 gaagaagaag ggctgaaaac acgcgtttca tcattcctcg gcgctatcgt gcagattatg
    23161 ctgctggata ttatcttcag cctcgactcg gtgattaccg ctgtgggtct gtcagatcac
    23221 ctgtttatta tgatggcagc cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc
    23281 tcgattggtg attttgtcga acgccatcct tcggtaaaaa tgctggcgct ctctttcctg
    23341 attctggtgg gctttaccct gattctggaa agtttcgaca tccacgtacc gaaaggttac
    23401 atctacttcg cgatgttctt ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa
    23461 aagaatccgc tctgatactc cgtacgctct cctgcgggag ggcgtactca ctccttttac
    23521 cctcacagtt taagattttc ctgctttcaa gatatataac gtcggtttat aaacagacta
    23581 ttatcatagg taggccaaat gaggataacc ggatgaaaaa atgggcagta ataatttctg
    23641 cagtcggact ggcgtttgct gtttccgggt gttccagtga ttacgtcatg gcgaccaaag
    23701 atggccgtat gattttgacc gatggaaaac ctgaaattga tgatgatacc gggctggtga
    23761 gttatcacga tcagcaaggt aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta
    23821 ttgaacgtta acaaataagg tcagcatccg gctggcctta agatttttct ctttcccttt
    23881 tcccttccct ctgccatttt tatattcctt atgtcgtgat tataaaaagg aaacggctat
    23941 gcaatatcac cgtatacccc acagttcgct ggaagtcagc acgctggggc ttggcacgat
    24001 gacgtttggt gaacagaaca gcgaagccga cgcccacgca caactcgact atgccgtcgc
    24061 tcagggcatt aaccttatcg acgttgccga aatgtaccca gtacctccgc gccccgaaac
    24121 gcaagggtta accgaaacct acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa
    24181 gttaattatc gcctccaaag tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc
    24241 ggatcaggcg ctggatcgga agaatatccg cgaagcgctg catgacagcc tcaagcgcct
    24301 acagactgat tacctcgatc tttatcaggt gcactggccg cagcgcccga ccaactgctt
    24361 cggcaaactc ggttatagct ggacggattc tgcgcctgcg gtttcgctgc tggatacgct
    24421 ggacgcactg gcagagtacc aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga
    24481 aactgcattt ggcgtaatgc gctacctgca tctggcggac aaacacgatc tgccgcgtat
    24541 tgtcaccatt cagaaccctt acagtctgtt aaaccgcagt tttgaagtag gtctggcaga
    24601 agtcagccag tatgaagggg tcgaactgct ggcctattcg tgcctgggtt tcggcacgct
    24661 gaccgggaaa tatctcaatg gtgcaaaacc cgctggcgca cgtaatacgc tctttagtcg
    24721 gttcacccgc tatagcggtg agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc
    24781 cagacgtcat ggccttgatc ctgctcagat ggcgctcgcg tttgtacgcc gtcaaccgtt
    24841 tgttgccagc actctgctgg gcgcaaccac gatggatcag ctgaaaacta acatcgaaag
    24901 tttgcatctg gagttaagcg aagacgtatt agctgaaatt gaagcggtac atcaggttta
    24961 tacttatcct gcaccataaa gattaacgcc ggttaaaacc ggcgttaaat attaatgacg
    25021 gcgctgccag atccacagcg ccgttattgc cagcgcaaat agcgcaccga agccaatgcc
    25081 aatgggcacg accgggatgc ctaccattac cgccagcgag taaatgccca gcatcaacaa
    25141 catggcgctg ttttcgccaa ggttttgtac tgcaatcgca ttccccgccc cgacgctttt
    25201 tttaccccgc tcctgtagca acgcattgag cggaacgaca aaaaagcccc ccagcacgcc
    25261 aatcagcatc aacaaggcat aggctggcag cagctcgtgt tgcagggaga aaatcggtac
    25321 caccacgcca atcaaaatcc cggctggcat acagcgtgac acggtttcca gcgtaaccag
    25381 cttcgctgcc gcacctgcgc caaccacgat accaatcgct accatcgcgt tgagataggt
    25441 cggcgtagcg ttatcggtaa tgcccagcgc caccggtacc cacagcacca acaggaaacg
    25501 cagcgtgaca cccgctcccc agaataaact ggtgcccacc agcgaaaaac gcgtttcacc
    25561 attgcaccat agcgaggtgc aggcattcag gaaactgcgg gtcatgttga tgagattcca
    25621 ggactgcccc ggacgcgccg ccagtttggg aatgtagata ttggcaacga ccgcaccacc
    25681 gtaggccagt gcgcatgcgg ccagagcgac caggacatgc cagtcagcca gcacaccacc
    25741 ggctacggaa ccgagtaaaa tcgccgctat ggcagaagct tccattaaac cgttagcttt
    25801 tactaactta ctacccgtgg ttaattcgcc gagaataccg tatttcgccg gtgaataggc
    25861 tgcagcacca acacccacca gcgtatagcc gagaaacgga ttgataccaa agcagatact
    25921 ggctgcgccc agcagcttca ggccgttggc aaacatcatc acccggcctt tggcgaagct
    25981 atccgccacc tgcccgacaa acggcgccaa aagaatgtaa gcacctacaa acaccatttg
    26041 caggatgggc tggctccact ccggatagaa ctgcgctttc agtaacgcca gagtggcaaa
    26101 cagtagcgcg ttatcgccaa acgcagagag aaactgcgcc acgataaccg ctttcatccc
    26161 cttcgaccac aacgaagtgt tagtgtgcac tgactcactc atcgtgttgt tccgcttcgt
    26221 ctacccagct tttcaacgtg acaaagtcag gtttgccgct gccaagtaat ggcatctgtt
    26281 tcagatagcg aatatcgcgc ggtacagcaa gctccggcac gccgtgctcg cgggcatact
    26341 gttgcaactt atcgcgcgtc agttcgttat ctgtggtgaa aagcaccagt gcctcgcctt
    26401 tgctggcatc gctcttaatc gcagtggcat ggactttatc tggcgaaaca ccaagtgcca
    26461 gttgttccac catttccagc gacaccattt cgcctgcaat tttggcaaag cgttttgcgc
    26521 ggccctgaat ctgcacaaag ccctgctcgt caaaacgcac aatatcgcca gtgtcatacc
    26581 agccgcgctc catttcgccg cgaacattct cggcggtggg cacttccagt acacctggct
    26641 tctccacccg cagatagccg ttcattatgt tcggcccttt cagttgcagg cgtccgccct
    26701 cttcgatacc agggaccgac aacaggcgtg catccattcc tggtagaata cgccctaccg
    26761 taccgggttt cgccgccatc ggtacgttga tagaaacgac aggcgcgcat tcggtcacac
    26821 cgtagccttc aaggatgcgc aggccaaatt tatcctgcca aagctgtttg gtactttctt
    26881 gtagtttttc tgcgcctgcc accacatagc gtagacgata gaagtcatac gggttggcga
    26941 aacgcgcgta gtgaccgagg aaagtcgagg taccgaacaa cacggtgcaa ctgcggtcat
    27001 acaccaagtc cggcacaatg cggtaatgca gcgggcttgg ataaaggaac acttctgcac
    27061 ctgtaagcag tggcgtaaac aggcctaccg tcagcccaaa ggagtgaaac agcggtaatg
    27121 ccgacataaa gcgatcgttg gtggtgaagt cggcaatcgt tttaatctgc tcgacgttcg
    27181 ccagaatgct tttatggcta tggacgacgc cttttggatg gccttcagaa ccggaggtaa
    27241 agaggatcag cgcctcttct tccggctgct gttttacctg cgccaggcgc ggcatcagca
    27301 aatgagcgaa gatccatact ttgtcggcag tggtgacatc tgcttttaaa tcttccagat
    27361 agacccagcg cacctgagta agttgctccg gcagatgcca gagtttgcct ttatcgagaa
    27421 actggcggga agtgaagatg gttttgattt cagccgccgt aatagcactg gtcagccctt
    27481 ttaccccggc agtgtagttc atcattgcgg gaatgcggcg acgcgcgatg gccccaaaaa
    27541 tcactgccgc actgatgcct gcattgggca gcattaagcc gatgcgttcg ccttcaacac
    27601 tgtatttttc aaggatgcgt ccaacaaaca gcgttttcgt aagcaatttg cgataggagt
    27661 ctggggtaaa gttgacgtct tcgacacatt tcttcccggc tccgaagcgg tacattgcac
    27721 tcagtaaaga ttcgtacaga gtttcacgcg ggcgcaccgc catacgcgct tccatcatta
    27781 tttgatgcag catttcgcca gcgattttgc gacggtcacg ggcacgcggc gcatccggca
    27841 tcgccacctg cgttggaggc aaaatatgca gagtaatttg cgggaacaag cgacgtttaa
    27901 ccagaccttt caggcggctg aagtgcgtaa gttccgcccc ttcaatacgc acaggaataa
    27961 ccgttgcacc agacttcgcc gcgacaaaac ccgcgccatc gtagattttc atcagcgagc
    28021 ctgtagtggt gatgcgtcct tcagggaaaa tcaccactgg tcggccctgt tccaccagac
    28081 gtaccagatg tttaatagcc ataggttgcg tcgggtcgag aggaacaaag tcgataaatg
    28141 atttcagcca acgcatatac cactgttggc ttattgaggt gtaaacggca aacactggac
    28201 gcacaggtaa aaacagtcca agcaaaatgc catcaataaa agagacgtga ttaggcgtaa
    28261 ttagtacgcg ctcgcccttc agtgcctggg tgtcacccgt aacgcgaacg cgatacaaaa
    28321 cacggcacaa atttcgaaaa aagctaaaaa gcattccaac tccctttgct ctgattcagt
    28381 aaaagcgaat ggagggagat tacacgagat aaagaacgcg agcgacagta aattaggtgc
    28441 gaaaaaaaac ctgcgcatcc gcgcaggttg gtgcaagaga cagggtacga agagcgtacc
    28501 gaataatctc accaatcaat acctctggga tcttgattgt ggtctgcacg acgactcttc
    28561 gccagcgaga aaacgcaaag gaatgaaggg aaatgcaacg aggtgtgtaa attgtcggtt
    28621 actgttacag attgatgacc ggcaaaaaaa aacctgcgca tctgcgcagg ctggtgtaat
    28681 tcatgtgctc aacacgaagt tgacttcacc tatcaatacc tctgggatca ccactttagc
    28741 aacctgaagc caaacgccac cagcggtcaa tcgcaacagc gtttcgcaaa gtgtaaccaa
    28801 aggtttgcaa tctctttttt tgtcttactt atttgcgttt ggctcacatt cccacgatga
    28861 aaacacgcca ccccttgaac caacgggcgt tttccgtaac actgaaacaa tgtaagcgtt
    28921 tacccactaa ggtattttca tggcgaccat aaaggatgta gcccgactgg caggcgtttc
    28981 agtcgccacc gtttcccgcg tcattaataa ttcacccaaa gccagcgaag cttcccggct
    29041 ggctgtgcat agtgcaatgg agtctcttag ctatcacccg aacgccaacg cccgtgcgct
    29101 ggcgcagcag accactgaaa cggtcggtct gatcgttggt gatgtttccg atccgttttt
    29161 cggtgcaatg gtgaaagcgg tcgaacaggt ggcttatcac accggtaatt tcttattgat
    29221 tggcaacggt taccacaacg aacaaaaaga gcgtcaggct attgagcaac tgatccgcca
    29281 tcgctgtgct gcgttggtcg tccatgccaa aatgatccca gatgccgatt tagcctcatt
    29341 aatgaaacaa atgcccggta tggtgctgat caaccgtatc ctgcctggct ttgaaaaccg
    29401 ttgtattgct cttgacgatc gttacggtgc ctggctggca acgcgtcatt taattcagca
    29461 aggtcatacc cgcattggtt atctgtgctc taaccactct atttctgacg ccgaagatcg
    29521 tctgcaaggg tattacgatg cccttgctga aagtggtatt ccggccaatg atcggctggt
    29581 gacatttggc gaaccagacg aaagcggcgg cgaacaggca atgaccgagc ttttgggacg
    29641 aggaagaaat ttcactgcgg tagcctgtta taacgattca atggcggcgg gcgcgatggg
    29701 cgttctcaat gataatggta ttgatgtacc gggtgagatt tcgttaattg gctttgatga
    29761 tgtgctggtg tcacgctatg tgcgtccgcg cctgaccacc gtgcgttacc caatcgtaac
    29821 gatggcgacc caggctgccg aactggcttt ggcactggcg gataatcgcc ctctcccgga
    29881 aattactaat gtatttagtc cgacgctggt acgtcgtcat tcagtgtcaa ctccgtcgct
    29941 ggaggcaagt catcatgcaa ccagcgacta accgcagtta aagcaattcc agcgccagta
    30001 attcttcgat ggtctggcga cggcgaatca accgcgcctg accattatca aacagaactt
    30061 ctggtaacag cggacggcta ttgtagttgg atgacattga tgcgccatat gcccctgtat
    30121 catgcagtac cagataatca cctgccttca cttccggcaa ggcgcgggtt tcaacatttc
    30181 ccccttcctg ctgggtaaag acatcgcccg attcacataa cggcccggcg acgacggttt
    30241 ccaccgttgg cgcgtgttcc agagaacgac catcagctgc cagggcactg atatggtggt
    30301 aactaccgta cattgccggg cgcatcagat cgttgaaccc ggcatcaacc agcacaaagt
    30361 ggcggctacc catttgtttg acgctccgca cctgagtaat taatacgcca gactgcgcta
    30421 ccaggaagcg acccggttca atttccagtt tcacagggtg gcccaaatgg cgagcgattt
    30481 gctcacgcgc ggcattccac agaccataat aatgttcggt atcaaccgcc tcttcaccct
    30541 gttgataagg aatagaaagc ccaccgcccg cagaaatagc ctgtaaatcc tgaccgaatt
    30601 cgataacctg acgcaccata gcaccacaca cctgctccag atgggcataa tcaacgccag
    30661 aaccaatgtg catgtgaatg ccgactagct gcagatgatg acgttgtatc acgtccagtg
    30721 cggcgggcag atcggtgtac cagataccgt gcttgctgtt ttcgccaccg gtattggttt
    30781 tttggctatg tccgtgacca aaccccggat taacgcgcag ccatacccga tgccctggcg
    30841 aaacctggcc cagttggtcg agcgtatcaa cagaacccgc attcaccgga atttgcaatt
    30901 cactgacgcg ttcaagcgtc gcctgatcga taacatctgc cgtaaaaaca atatcatcgg
    30961 ggtgcgtttg cggattgtaa cccgccgcca acgcacgctc tatttcgcct aacgagacgg
    31021 aatccacttt cacgccctgc tcacgcatta agcgcaaaat atgaatattg gaacaggctt
    31081 tctgtgcaaa gcgcaccact tcaaactgtt tcagcgctgc aatctgccga cgaataattt
    31141 gcgcatcgta gacccacacc gggcagccaa attcagcggg caaacgcagc agattttcgg
    31201 cggtgagatc ggtatcggtg ctgaacagtg aatgtggcat aacaaactcc agatgagtgc
    31261 ttttttatga ttacgccaca tcataaaaag aataaaaaat atcgatttat gtcgagtcta
    31321 tgcaaaaatg atatggatta ccggattgcg agagagcgct aatggccgcc gttaacttac
    31381 gtcatattga aatttttcat gcggtaatga ccgccggaag cctgactgag gcagcacacc
    31441 tgctacacac ctcacagcca accgtcagcc gcgaactggc gcgctttgag aaggtgatcg
    31501 ggctgaaatt gtttgagcgc gtacgtggac gattacatcc taccgtgcaa ggactgcgtc
    31561 tgtttgaaga agtgcaacga tcctggtacg gactggatcg cattgtcagc gccgcagaaa
    31621 gtctgcgcga gtttcgccag ggagaactgt ctattgcctg cctgccggtc ttttcgcaat
    31681 cttttttacc gcagctcctg caaccctttc tggcacgtta tcccgatgtc agcttaaata
    31741 tcgtgcccca ggaatcaccg ctacttgaag agtggctctc ggcccagcgt catgatttag
    31801 gactcactga gacactccat acgcctacgg gaacagaacg taccgaatta ctctctttag
    31861 atgaagtgtg tgtgttacct ccgggtcatc cgctggcggt aaaaaaggta ttaacgccgg
    31921 atgattttca gggtgagaat tacatcagcc tttcccgtac tgacagctat cgccagttgc
    31981 tggatcagct atttactgaa catcaggtta aacgacgcat gatcgtagaa acccacagcg
    32041 ccgcgtcagt ctgcgcaatg gtacgggcgg gggtaggtat ttcggtggtt aacccgctca
    32101 ccgcactaga ttatgcggca agcggtttag tggtgcggcg gttcagtatt gcggttccgt
    32161 tcaccgtcag cctgatccgc cccctgcacc gcccgtcatc agcgctggtt caggcgttta
    32221 gtgggcattt acaagcgggg ttaccgaaac tggtcacttc tcttgacgct attttgtcgt
    32281 cagctacgac agcataaaag cgacagcatc ctcggcatgg atcgccgcgg tatcaaacac
    32341 aggcaaaaca ctgcgctctt ctggcaccag taaaccaatt tctgtgcagc caaaaatgac
    32401 gccctgtgcg ccctgttctg caaggcgagc aatcacttgc gcataataag cgcgtgacgc
    32461 ttcggtaaat tgccccagac acagttcttc aaaaataatc tgattaattt tcgcccgttc
    32521 atccgcttca ggaataagac aattgatgga aaattgttcc gtcagccgcc cgcgataaaa
    32581 atcctgttcc atggtgtaac gcgtacccag cagcgccaca cgagtcattc cggccccggt
    32641 aattgcacgt ccggtggcat ccgcaatgtg taagaaaggc agagtgcaac gtgactcaat
    32701 ggcatccgcc actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg
    32761 ctgtaagcca agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg
    32821 ctggcactct tctatttcat gaaaatcgac gctatgtagc agcatttgcg cagagtgaag
    32881 cccaccaagc cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc
    32941 ccagctcatt cctcccagca aaccaattgt tttcattcct ctctcctgat gtgtgttacc
    33001 gcaacaaggc tgaataagga acgagacaaa cgcctcaacg gccaggtgcc caatctctat
    33061 taacgaaaaa agggccggat gtacagcaca tccggcccgt gaaatcagac gccgatattt
    33121 ctcaacttct cgcctgccat cagtttgcgt tcgatatgtt ccagcgtgac atttttggtt
    33181 tccggaatga gccagaaagt aatacccaca aacgcaatgt tcagcgcagt gtagagccag
    33241 aacgtaccgg cagcgccaat gctatcaagc agtgtcagga aggccgcgcc gataatcata
    33301 ttcgacaccc agtttgtcgt cgtcgaacag gtaataccga aatcgcggca tttcagcggc
    33361 tgaatttcag agcacaggat ccacaccact ggcgcggcgc tcatcgcata accggcaata
    33421 cacatcatcg tcatgccaac agagagccag gacaagccac tggaagccgt accgttatca
    33481 aactgcatca ggcaatagcc cagcaccaga gtgcctaacg ccatcacgct gaaaccaatt
    33541 ttcagagccg gtttacgacc tgctttatct accgtaaaga ccgcaatgaa ggtcgcgaac
    33601 ataaaggtca gtcccacgac cagagtcgca atcatctgtt gttctgtggt cgtaaagccc
    33661 gccattttga agatacgcgg cgcgtagtac atgatgatgt tcataccggt aaactgctgc
    33721 atcgcctgca acaacatacc gagaaacaca gcacgacgga cgttacggtt gatcttaaac
    33781 agtgcccaac cgccctgttt taacttcagg ctttcacgaa tttcgttgag ttcttctcgc
    33841 gctttttccg acgtatcgcg cagcatacgc aacacttctt ccgcctcaat atgacgcccc
    33901 ttttccgcca gccagcgcgg gctatttggc aggaagacca ccagaataat cagcagaact
    33961 gctggtaaag caagaacccc caacattgcg cgccagttac cgctataact gaacgctgta
    34021 tcggataaaa acgccagcac gatgccgagt gtgaccatca actggtacat actgatcatc
    34081 ttaccgcgaa cgttttcact tgccatttca gaaagataca gaggagcggt gtaagacgcg
    34141 atcccgacag caatgcccag caccacacga gcggcgatta acatctctac gctggtcgca
    34201 aaagcggacc ctatagaacc gagtacaaac aggatggccc ccgccatcag gctgtattta
    34261 cgccccaggc ggaacgacag ccaaccatta aacagcgcac caattgctgc gccgagcatc
    34321 atgctactaa ccacccattc ctgcaaacga ctggtcagca caaagtgatc ggtaatgaac
    34381 ggcaacgctc cggcgattac gccgatatca agaccaaata acaatcctgc gaccgcagca
    34441 gctaccgaaa caaacatatt catacgccgc gtatcacgca aagaacgtgg cgttaaagca
    34501 gattccgtat tgatagtaac cattttttcc tgccagcaga gagtaagaca ttgtgaaaaa
    34561 atacgtgaac aattcacaca ggtgtcaggt cggaaacagc ataaatatgg attaaattgc
    34621 tgcgacatgc cgttatgtga tggatattcc aattttaaaa ttaagttgaa ttattgagat
    34681 tattattaac ctcctaattt taaagcagat aaaattcata aagttcatta attgataatt
    34741 gatatggatt atttcataac catgatatgg attatgatga tctagagata taaaaaaccc
    34801 tgccatgcgg cagggtcata aaagtaagag gaatgaatta acgcgccagc caaccgccat
    34861 ccacagcaat ggtataacca tttacataat ctgaagcgct ggaggcaaga aacactaccg
    34921 gccccatcag gtcactcggc agtccccaac ggccagctgg aatgcgatcg agaatttccg
    34981 cgctacgttg ttcatctgcc cgcagttgtt gggtattgtt ggtcgccatg taacccggag
    35041 ctatcgcatt aacattaatg ttgtgtttag cccattcgtt tgccatcaat cgcgtcacac
    35101 ccatcacgcc gctttttgat gcggtataag aaggcacacg gatcccgccc tggaaggaga
    35161 gcattgacgc gatattgata atcttgccgc cattgccttg cgcgataaag tgtttcgccg
    35221 ctgcctgaga catgaagaat acgctcttga tattcaggtt catgacatcg tcccagtctt
    35281 tttcgctgaa ctcgagagcg tcttcgcggc gaatcaatcc ggcgttattc accaggatat
    35341 caatatgacc aaattcagct accgcgcgat ccagcagtcc aggaatgcca tcaatctttc
    35401 gcagatcggc ggtcaggctt aaaaaacgac gccccagcgc cgtgacctgc tcgatggttt
    35461 cagtcggttc aacgatgtta atgccaacaa tgtcacagcc cgcttgcgcc agccccaacg
    35521 ccatcccctg gcccagccca gtatcacaac cagtgacgac cgcaacttta ccttggagag
    35581 aaaatgcact taaaatcata acaacacctt attcgtttat gcccacaact agcgcaaatc
    35641 tttaacgccc acatggtcca tatcattaaa gacctggttt tcaccgacca tgccccagat
    35701 aaaggtataa gctttggttc cgacgccgga atggatcgac cagctcgggg agatcaccgc
    35761 ctgctcgtta tgcatcacaa tgtgacgcgt ttcttgcggc tgccccatca tgtggaaaac
    35821 gcaggcgtca tcatccatat tgaaatagaa ataaacttcc atccggcgct cgtgggtgtg
    35881 acacggcatg gtgttccaca agttacctgg agccagctcc gtcagcccca tactcaattg
    35941 acaggtttcc agtacatccg ggacaaaata tttgttaatc gtgcgacggt tactggtgag
    36001 gttatcgcct aacgtgactg gagatacttc gtccggtgtg acttttttgg tgggatacgt
    36061 cgtatgcgcg ggtgcgcaat tgtaataaaa cttcgccgga gtggcggtat caatactggc
    36121 aaagacaact tcttttgcac ctttaccaac atacagggcg tcgcggtgac cgatttcata
    36181 gcattgacca tcgacagtaa tcgtaccggc accgccaata ttgataacac ctaactcgcg
    36241 acgttcaagg aaatagctta cgccgagttg tttaccaact tccccgccaa cggaaaccgt
    36301 tttagttatc ggcataatgc cgccaacaat aattcggtca atgtggctgt aaaccatggt
    36361 gtactcatcg gcgacaaata ccttttcaac caaaaattca ttgcgcagcc cttgggtatc
    36421 cagcgttttt gcgtgcgcac cgtggatgct ctgtcttacg tccacatcaa cctccgataa
    36481 tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg tttcgttttc tctataaagg
    36541 gatgatatgc ccatgggcta gggttttcaa ttaaaaataa aacaatgttt tatttttaat
    36601 ttaaggatta aaaaaagtgc agtgtcgatc acgaatatgt catgagaatg gaaaaatgag
    36661 ggagcccaga aaatgtatgt cgacttgcca gccatcatta ttcaacagat taatgacggc
    36721 agatatttat cggtaaggag gaaatgcaga gctactcgtc acgttcaatg gtcaatgcca
    36781 cgccctggcc cccgccgata caaagcgttg ccagcccttt gcgggcatta cgtttcacca
    36841 tttcatgaac cagagaaacc aggattcggc aaccggaagc gcctatcggg tgaccgagtg
    36901 cgatcgcgcc accattgaca ttgacccgac gctcatccca ctcaagcatc ttgccaaccg
    36961 aaagcgcctg tgcagcaaac gcttcattag cctcgataag atcgacatca gccaactgcc
    37021 agcctacacg ctccaggcaa cggcgggtcg catacaccgg cgcaattccc atcaatgccg
    37081 gatctacacc aacgctggca aatgcgcgga tgcgggccag cacgggtaaa ttcaacgctc
    37141 gtgctttggc ttcgctcatc atcattaccg cagctgcgcc atcgtttatg gatgatgcat
    37201 tccccgcggt cacagaaccg agactatcaa atgaaggatt taaacgggct aaaccttctg
    37261 cactggcgtc agtgcgtggc tgttcatcgg tatcaacaac caacgtctga ccgttacttt
    37321 ggatcattac cgggacgatc tcatctttaa atcgtccggc gtcaatcgcc gctcgcgctt
    37381 tttgttgcga gctaagtgcg taagcatcct gcaactgacg gctgatgcca tattcgcgag
    37441 ccagattttc ggcggtgaca ccaatatgat aatcattgaa ggcatcccac aacccatcat
    37501 gcaccagact gtcaaccaac tggctattgc caagctgttc accggtgcgg ctatcagtca
    37561 gaacatgtgg tgcgcggctc atgttttcct ggccacctgc gatgacaata tcagcctcgc
    37621 cacactgtat cgcctgagta gccagatgca gtgctttaag accggaaccg caaacgtcat
    37681 taatcgtgat tgcagaaacg ctgttaggaa gaccaccttt aatagccgat tgccttgccg
    37741 gattctgccc tgcccctgca gtcaacacct gaccaagaat tacttcatcc accgcatatg
    37801 caggaacgcc ggtacgttct attaacgctt tcacgaccag actaccaagt tccacggcgg
    37861 aatgacccgc taacgcacca cgaaagcagc cgataggtgt ccgtaacgcc ccgacaatca
    37921 caacgtcttt catcacaacc tcgcaccaat taacagtgca atagtaaatg attgttaatt
    37981 acagttatct taattgttta aaaaaagtga tttttatcac aaaggaaata tgcctgagca
    38041 gtagtcagag tcataactgg cacgtaaggt ttgcaaccac taacccacca atagatgggt
    38101 agatagggcg ttaatctccc atacttaact tggtttatgg taaattgccc tccattttgt
    38161 ttaatttgta gatgatacgt tcagataatg tctaatattt ggtctaaaga agaaactctg
    38221 tgaagtttcg cgctctatgg cacagccgtt gatgcaggca cgctattcct tcctattcag
    38281 ttaggttcgg caggggctgt ggtcctgttt attactgctc tggtcgcctg gcctttaaca
    38341 tattggccac ataaagcctt atgccagttc atcctctcat caaaaacatc agcaggtgaa
    38401 gggataacgg gcgcggtaac acactactat ggcaagaaga ttggtaatct gattaccacg
    38461 ctgtacttca tcgccttttt tgtcgtcgtg ttgatatatg cagtggcaat taccaactca
    38521 cttacggaac agctggcaaa gcatatggtt attgatcttc gcatccgtat gttggtgagt
    38581 ctgggtgttg tattaattct gaatctcatt tttctgatgg gacgtcatgc cactattcgg
    38641 gtaatgggat ttttggtatt cccattgatt gcctatttct tatttctttc catttacctt
    38701 gtcggtagtt ggcaacctga tctattaaca acccaggtag agttcaatca gaataccctt
    38761 caccagatat ggatatcgat tcccgtgatg gttttcgcct ttagccatac gcccattatt
    38821 tctacgtttg ccatagacag acgtgaaaaa tatggcgaac acgctatgga taaatgcaaa
    38881 aaaattatga aagtcgctta tctcatcatc tgcataagtg tactgttctt tgtctttagc
    38941 tgcctgcttt ctattccatc ttcgtatatt gaagcggcta aagaagaagg ggtcaccatt
    39001 ttatcggcgc tttctatgct gccgaacgcc ccagcatggt tgtcaatttc cgggattatt
    39061 gtcgcagtag ttgcgatgtc gaaatcattc ctgggtacgt actttggcgt tattgaaggt
    39121 gccacagagg tcgtcaaaac aacactacag caggttggtg taaagaaaag tcgtgcattt
    39181 aaccacgcac tatcaattat gttggtatcg ctgattacct tcattgtttg ttgcattaac
    39241 ccgaacgcga tttcgatgat ttacgcgatc agcggcccgc tcattgccat gatacttttc
    39301 atcatgccta cgctgtcaac gtatctcatc ccggcgctta aaccctggcg ttccatcgga
    39361 aatctgatta cgctgatcgt gggtatcctg tgcgtatcgg taatgttctt tagctaatat
    39421 ccgttgtgga tggttcaaaa ttgctacaat tttgaaccat tccatttttc ataatattca
    39481 tttgttatta ttccgcccta tcacttctct cgtcatatcc tagaattatc agttagttaa
    39541 acacacaaag agatctgaac atcttaaata agactattta agatgcataa cttagattcg
    39601 caagatatct tctggtcatt caaaaacaat ttgcgatata gatattctca taccatgaga
    39661 tagttatctg gaccacgttc gaccatgcaa gattaaatca gtgaaacata atcatattta
    39721 ataacgagaa atgcattttt aaatatctta atcagaatta tagcaaatac agattaatcg
    39781 caatttttcc cagcaaaatt acaaaatcaa ggtgtattga catcatcaat caccaatgaa
    39841 attatgcaat tatatacgga tagggaggtt cttaacatgg ggctttgtag tcgttataaa
    39901 agtcttacat gcaatagttg ctcaatgcat tgccaaataa tgccagaaga gtcaccgcgt
    39961 ttgcagtact gcgcaaactc gtgtt
//
LOCUS       DAJTSF010000027        38786 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000027 DAJTSF010000000
VERSION     DAJTSF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.027"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="OO335_001492"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="OO335_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR6001933.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..571
                     /gene="hisL"
                     /locus_tag="OO335_001493"
     CDS             530..571
                     /gene="hisL"
                     /locus_tag="OO335_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6001934.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    547..670
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="OO335_001494"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="OO335_001494"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6001935.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="OO335_001495"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="OO335_001495"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6001936.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="OO335_001496"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="OO335_001496"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6001937.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="OO335_001497"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="OO335_001497"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6001938.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="OO335_001498"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="OO335_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6001939.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="OO335_001499"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="OO335_001499"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6001940.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="OO335_001500"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="OO335_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6001941.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /locus_tag="OO335_001501"
     CDS             7139..7750
                     /locus_tag="OO335_001501"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6001942.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="OO335_001502"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="OO335_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6001943.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="OO335_001503"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="OO335_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="OO335_001504"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="OO335_001504"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6001944.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..12303)
                     /locus_tag="OO335_001505"
     CDS             complement(11944..12303)
                     /locus_tag="OO335_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001945.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12300..12806)
                     /locus_tag="OO335_001506"
     CDS             complement(12300..12806)
                     /locus_tag="OO335_001506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001946.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGERCILN
                     SNIFILNT"
     gene            complement(12952..13515)
                     /locus_tag="OO335_001507"
     CDS             complement(12952..13515)
                     /locus_tag="OO335_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001947.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13722..14660)
                     /locus_tag="OO335_001508"
                     /pseudo
     CDS             complement(13722..14660)
                     /locus_tag="OO335_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14723..15097)
                     /locus_tag="OO335_001509"
     CDS             complement(14723..15097)
                     /locus_tag="OO335_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR6001948.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15101..16249)
                     /gene="wzy"
                     /locus_tag="OO335_001510"
     CDS             complement(15101..16249)
                     /gene="wzy"
                     /locus_tag="OO335_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR6001949.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16269..17174)
                     /gene="rfbG"
                     /locus_tag="OO335_001511"
     CDS             complement(16269..17174)
                     /gene="rfbG"
                     /locus_tag="OO335_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR6001950.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEKQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17167..18057)
                     /locus_tag="OO335_001512"
     CDS             complement(17167..18057)
                     /locus_tag="OO335_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR6001951.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18087..19343)
                     /gene="wzx"
                     /locus_tag="OO335_001513"
     CDS             complement(18087..19343)
                     /gene="wzx"
                     /locus_tag="OO335_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR6001952.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19359..19904)
                     /gene="rfbC"
                     /locus_tag="OO335_001514"
     CDS             complement(19359..19904)
                     /gene="rfbC"
                     /locus_tag="OO335_001514"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR6001953.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19909..20787)
                     /gene="rfbA"
                     /locus_tag="OO335_001515"
     CDS             complement(19909..20787)
                     /gene="rfbA"
                     /locus_tag="OO335_001515"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6001954.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20846..21745)
                     /gene="rfbD"
                     /locus_tag="OO335_001516"
     CDS             complement(20846..21745)
                     /gene="rfbD"
                     /locus_tag="OO335_001516"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR6001955.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21745..22830)
                     /gene="rfbB"
                     /locus_tag="OO335_001517"
     CDS             complement(21745..22830)
                     /gene="rfbB"
                     /locus_tag="OO335_001517"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6001956.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(23203..24096)
                     /gene="galF"
                     /locus_tag="OO335_001518"
     CDS             complement(23203..24096)
                     /gene="galF"
                     /locus_tag="OO335_001518"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR6001957.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24271..25665)
                     /gene="wcaM"
                     /locus_tag="OO335_001519"
     CDS             complement(24271..25665)
                     /gene="wcaM"
                     /locus_tag="OO335_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR6001958.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25676..26896)
                     /gene="wcaL"
                     /locus_tag="OO335_001520"
     CDS             complement(25676..26896)
                     /gene="wcaL"
                     /locus_tag="OO335_001520"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR6001959.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26893..28173)
                     /gene="wcaK"
                     /locus_tag="OO335_001521"
     CDS             complement(26893..28173)
                     /gene="wcaK"
                     /locus_tag="OO335_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR6001960.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28353..29831)
                     /gene="wzxC"
                     /locus_tag="OO335_001522"
     CDS             complement(28353..29831)
                     /gene="wzxC"
                     /locus_tag="OO335_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR6001961.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29833..31227)
                     /gene="wcaJ"
                     /locus_tag="OO335_001523"
     CDS             complement(29833..31227)
                     /gene="wcaJ"
                     /locus_tag="OO335_001523"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR6001962.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31282..32652)
                     /gene="cpsG"
                     /locus_tag="OO335_001524"
                     /gene_synonym="manB"
     CDS             complement(31282..32652)
                     /gene="cpsG"
                     /locus_tag="OO335_001524"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6001963.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32845..34281)
                     /gene="cpsB"
                     /locus_tag="OO335_001525"
     CDS             complement(32845..34281)
                     /gene="cpsB"
                     /locus_tag="OO335_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6001964.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34284..35507)
                     /gene="wcaI"
                     /locus_tag="OO335_001526"
     CDS             complement(34284..35507)
                     /gene="wcaI"
                     /locus_tag="OO335_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR6001965.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35504..35983)
                     /gene="gmm"
                     /locus_tag="OO335_001527"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35504..35983)
                     /gene="gmm"
                     /locus_tag="OO335_001527"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6001966.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35983..36951)
                     /gene="fcl"
                     /locus_tag="OO335_001528"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35983..36951)
                     /gene="fcl"
                     /locus_tag="OO335_001528"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6001967.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36954..38075)
                     /gene="gmd"
                     /locus_tag="OO335_001529"
     CDS             complement(36954..38075)
                     /gene="gmd"
                     /locus_tag="OO335_001529"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6001968.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38101..38649)
                     /gene="wcaF"
                     /locus_tag="OO335_001530"
     CDS             complement(38101..38649)
                     /gene="wcaF"
                     /locus_tag="OO335_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6001969.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38665..>38786)
                     /locus_tag="OO335_001531"
     CDS             complement(38665..>38786)
                     /locus_tag="OO335_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR6001970.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10353 a         9713 c         8729 g         9991 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggatcaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgagatatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcacttgct ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt
    12301 caagtattca gaataaaaat atttgaattc aaaatacaac gttcgccccg aaaaaaaata
    12361 atacacacaa ggataaaata tcgtcccaac ttgtaatttg taatgttgat atcttttatt
    12421 aaacttgcaa ttgagatcgc aacctgcaat actgacaatg ggtaaatggg tgataaatac
    12481 ctgtcacctt gcgtaacaga aaaccaaaat aagacatatg ttaaaacaat cgagaaaaat
    12541 acccaaagta ttttattatt cctaagtgta aagacagaca gtatagcata aaaaacaaca
    12601 tcactgccaa ctttattaat tgcttgttgc aaatctaatg tggcaggact tataccatgt
    12661 cgagcttgtt ctgctgtttg aacttgtaga tcaatttcat tccataggta gtgaccaaat
    12721 atatttcctc cagcaggact aaaaggatca ccagagatcc aaaaactacg gacataccaa
    12781 taaatgccac caagaattgt tgccaagcta aaaataagaa atgtctgtat gtttctcttt
    12841 ttcgctatca atataacaaa tatgatggct ccatatatgg caccaaatag ttttacgcca
    12901 caagcaattc caataaaaaa accacataaa aaaattaatt cattttgtgt tttattgttt
    12961 tctgagcgaa tatatataag aatactggct gcagcaaaac aaagatagcg ttaaatcaat
    13021 atatgcataa ccaagatgat ctttaagtgg attgatagta aatagtagga ttgtagcaat
    13081 aatgcctgga taataagttt tcgtaatttc ccatgaacag ccaacaagtc cacaggctat
    13141 tacaaaaaaa cagatatttg ctaaaaactg agctaacctt acatctccta actgtaaacc
    13201 taaagccatt aaaagatctg cattttgcgg gaacagagga aaacgcaaat attgttcaac
    13261 aacaattgca tggtgctcaa ctaatgaacg agccaaaggt aagtgataca tagtatcatc
    13321 ccaatcaccg ggagctcgca gggacgataa caatgtaacc acaaaaatca aaacaagaaa
    13381 taaaataaca aacagcgtac tgaactcaat attatttatt gccagccatc taaattgctg
    13441 acgtttaata atgcttaaaa cccaacacac cgtaaaaatt aaaaagatag caatcttatt
    13501 aaattgcccg accatgccca gaaagtaaat tatcaaaata tttaaagaca tccctatgca
    13561 taggcaataa aaaataaaga aaatttgtta agtcctgcca ccccctcctt aaaaaaagat
    13621 gacaatacaa tattaccaat gacaaaaaat gtcattccaa aagcaaaaca atacacaaca
    13681 tacaaataca acggcaattc aatcatagta aaatactcta tttacattat cagtttatag
    13741 tttttttcag aaagataaaa taatgccttc atttcacgtc tagttgttgt atttgaatgc
    13801 aaaataatgc cacatacaaa tgccattatt gacgataaca tgatggaggc agttaaaaag
    13861 gcagtcggaa atcttctcac caaacctgta tcaatataat caacaatgat gggcaatcct
    13921 aaaattatag acaaagcagc aagtaaaacg ccgataatac taaaaaagta tagtggccgt
    13981 tcaagcgagt aaagtttaat tattgttttc agaatcttga acccatcact ccaagttgat
    14041 agtttgctca cactcccttc agatcgctcg ccatattttg tattaacctc accatacttc
    14101 atacgtaatt ctaaagcatg aattgtaagc tcagtttcga tttcaaatcc ccttgataga
    14161 catggaaaag atttaacata gcgtctggaa aatacacgat accccgacaa catatcagta
    14221 aaattccctt taaagatagt ctgaacagta ccagtcaaca atttatttcc tcagcgatgc
    14281 ccttttctgt atgtattttg atctccattc tcttgacgac atcctacaac catatccaat
    14341 tgatttttta tgagatggtt aatcatttct ggagcagaac ttgcatcata agtgtcatct
    14401 ccatcaacca ttagataaat atccgcatct acatctgaga acatacgtct tacgacattc
    14461 cccttgcctt tcagaggaac agagtgaact gttgctccag catcctctgc cacttgtgct
    14521 gtactgtcag tcgaattatt atcaaataca tatatagaag cagttggaat attcgttttg
    14581 aaatcattta tgacttttgc tattgccttc tgctcattat aacaaggaat aattacagcg
    14641 atattaaaag agtgatacat aagcccctca tataaactat ttagctaatg tgcaagtaat
    14701 tgtgttttta acaaatcgtt tactaacgaa aaatgaaata ttttgatgcg caatatccta
    14761 aaaccaaact aaaaagagaa aaggtgaaca atgtaaatat tggagataaa gctaagagat
    14821 caaataaaac gccgaataaa taactcatcg cccccataaa aaatataaat ataaaatatc
    14881 tagccttggt tgcagatact ttaaatgagc atcttgcatt tgcaaaaaaa gaaaaggttg
    14941 ctgcacatat aaaaccaaca atgttacttc ggccttgacc aaaagctaaa ttattataac
    15001 acgcataaaa taccccccaa tgtaaagcag tattgacaat tccaattaaa aaataactaa
    15061 aaaaagaact ggtcaataac ttccctattt ttaacatcct ttattttgct ccagaagtga
    15121 ggttattact aatttggata ttttctatag aaaataccca taaaactaca acgcctataa
    15181 taaaaattcc catgggtgta aaaagaaatg gattagttat agaaacggta atactgaaaa
    15241 aaagtattaa cgacatcatt aatgatgttt ttgtactatt taaaaaatag cctttcaaaa
    15301 agataagcaa cataggaact aatgatataa atacaccaat aacgcctgtt ttctgaagta
    15361 tctcaagagg tactatttca atccttaatc gatctaatat aaatgaacca aatcctcttc
    15421 caaacaagaa cgtcgctaaa tcaacatttt tataataaaa atataaatca ttaaatctaa
    15481 cagagtcaga atcttcactg cgtgatccga ccatgtaaag cataattata aatagaacgc
    15541 tgcccaatat tattataata gtatttaaat atagcttatg taatttaaaa ctggctaata
    15601 acagtgataa tgttgtaaaa agtaatacac ctcttgattc tgtcaataat atcgcaagca
    15661 ctattatgag acattttaaa tattttttgt cgacaaaact gataatcgca ccgacaccaa
    15721 aaaagtagaa ggatttggaa aaaaaagccc catcgggacg aaaaaagaac tcgctagtca
    15781 atgataagtg ttcataaatt aaattaaaat ttaacaaacc gaaattcaac aaaattatgt
    15841 atgaaatata taacagtgat ccatataatg caaatgcatt aactatttta ataaaaatct
    15901 cacatgaaaa tacttttaaa gtatatctta gtgtaaatag atatataagt gacagcataa
    15961 aaaggtatgg ttgcacatca aacagtatat catttatttt attaccagct aataagccga
    16021 caaagaagcc ataaaaagga aataaaagca accatataaa aagactgaca catttttggg
    16081 tgattgaaaa attgaatgta actaaattaa aaattaaaag tataaaacta aatacaaata
    16141 ataaatatcg caaagggaat attcccaatg gctccagtaa tcttccacca ccaccaataa
    16201 tcagttcaat acataaaaat gttataaaaa ttttatttat attattcata ttcgtaaggt
    16261 gatgtttttt aaaatatgtt taaaatcaac attaataaaa tataaatttt atcagccttt
    16321 ttccgcctta acttccaaaa acttaaagcc aagatcaaat tgcctatata acctttaccc
    16381 aattgattat tcacaaagct atcatcagct aaggcattag ctattttagt tacttctttt
    16441 cgataccact tgtttctcac caaaccaagc cgtttgtatt ttgctttaaa actaatgttt
    16501 gctcccactt ggttgttttc atgctgtctg tacagcattg ttggttttct atcaatatac
    16561 cacgaataat ttctcgtccg cgcaaatgaa tataaaaacc aatcatgcag acagatatct
    16621 tgtgcagcat ttttattatt tatgatgaac ttcttaaatt caataagcgt ttcttttttt
    16681 ataacatatg tacaccccgg acctgctgct tcaaaaaaat aatcgaattg agtttgcgac
    16741 tgagccttat caactaaatt ttttcgccct gatggataat atgcaattac attgctggaa
    16801 taacaattcg cattattaaa taccaagcag tcaattgcat tttttatttt atctttatac
    16861 caaatatcgt cctgatcact aaaacagata taatcatagt tttcaagatc gacatcttta
    16921 attaatctat aaaaattttt acctgccccc ccaaacttat tgacgcttgg aagtatattt
    16981 ataattgagg gatatgataa ttgaaattct tcacaaatat ttaatgtatt atcagttgat
    17041 aaatcatctg atataaaaat tgagatgtct acatcttttt gttcaataat actttttaat
    17101 tgtttctcaa tccagcattc gccattatac gtagccataa gtacagctac tcttttttga
    17161 tcagaactat gcattataac gaccgccccc agtaattcct cttattccat ccagcgtagc
    17221 caaaatatgt tttttcatat attcaatttt atttttttga gttgcgataa ttaaagtcgt
    17281 atgaattaaa gaaaaaacaa cttccctaat agagagtaaa agaggcacat gattttttct
    17341 aagcaaaaga aatgcatttc ttactcgata ataacgacgt actggcgagt gaattggtat
    17401 gttaatccca cagattttta ctgacttatc cccaatagca tgaaccattt ctatatcagg
    17461 aatgatatga actaatatgc catagtttaa gcatctaaga caccattctg tatcgacata
    17521 atcaataaaa agttcctcat ccatcattcc aacaatctca aaaacttctt ttgaaaccat
    17581 ggttccggat gagattgtta ctgaactttt aaacggtgtc tgtccttcag tgacatgaat
    17641 tttctctcga agaccatttt ttttgatatt acaaatagga taataatgtg acttatctct
    17701 atcaataaaa actgggccag ctatttttgc attttcatta tccatatagc aggaaagttt
    17761 tttgataaac tcattgcgaa tacggctatc ttgatcaaaa aaaattatct tctctgcccc
    17821 tttttcaaga gaatatcgaa tcccaatatt ttgtgctttt gctatacctt cattcctacc
    17881 aagacaaatt aaatgaactt tttcaatgtc tgctaattta tctttcaagg tgaaattagt
    17941 cctattatcg actactacaa cgtaacaatt ttgctccttt agttcagtga tcagtgcatt
    18001 cagattttta agttcgggat tatatgtcac aatgacagcg taaatattac tattcattat
    18061 ccaagtgact cagtaattgg ttaattttag ttttcatata cagaacacat tctagtataa
    18121 agccattgta ataacagagc atctaaaatt acacgtatgc tccaggatat tgcggcaccc
    18181 agtaacccat atttttcgac cataaaatac aaaagggcta aataaggaac caattcagca
    18241 caatgaatca aagcagtaat tttagatttt cctaatgcct ggattgcaga gaatggtatc
    18301 attgccagcg cattgaagaa aaaaccgatc aataacacat ttaatatatt aaaggaatta
    18361 atttgataat tctcaccaaa ccatattttc aatacaagcc ctgaatataa caagcctatg
    18421 attatcaccg gcaaacagat tagaaacatt aaaagcaatg atttatttac attacgttta
    18481 aattctttaa agtctttgat gttacttaac cttggaaaca ctgccctccc gattgctgca
    18541 ggtattattc ctaattttag aataacctcc gctggtgcag aataaaatgc aactttatct
    18601 gcccccataa tatttgacac tataaatcga tcaaaataca ccatgaccgg acttatgata
    18661 ttacttactg tcatccaacc gccaaagaaa aagagtcgtt taaaggtttt ataacaaaaa
    18721 tgaacccccg aaattttaat atcattcctg acataatagg cagagactaa aatcgaaata
    18781 actcttgcaa atattaaacc agcaaccgct gccgacaatg tagcactata aaaaacaaat
    18841 atggccggaa ttcccgcgat gcaagagctt gatatagatt tttgaatatt tacaatgcca
    18901 aatttttcat ccccctcaag aatggctgac cataattgat ttagaataaa taatggaatg
    18961 caaatagcca acagtttaaa tgctagttgt atatcactat gttcaacacc agaaattttt
    19021 agataattaa caattccatc agagaaaatc aataataaaa aagcaccaaa gcatgaaaat
    19081 agcaccaaaa aagatgtact tgttgaaatt accttggttc tttcatgatg attatcacga
    19141 tgaattgcaa tttctctaat aacagagcga gtcaggccta catcaaaaat tccagcatac
    19201 ccaacaagtg caattgcaat tgtataaaca ccgaaacctt caggccccaa acctcgagcg
    19261 agaaatccta gcgaggggat agcaattaaa gttggtattg catagccaaa aaggttccag
    19321 acactatttt ttattatgct cattattaca tttctttatc atataaaata atcagcatct
    19381 acaaattttt gaccattaat atctttatca gaaagcaatg gattttgaac cggccattct
    19441 atatttatac ttttgtcgct ccataaaatc ccacgctctt gcatttttga atagaaatta
    19501 tttgttttgt agacaaattc tgcttcatca ctaagaacgc aaaacccatg agcgaatcct
    19561 tccggtatcc ataattgacg cttattctcc gccgacaaat ttacaccaac ccacttgcca
    19621 aatgttggtg atgatttccg aatatcaaca gctacgtcaa aaacttcacc gacaacacag
    19681 cgtaccagtt ttccttgagc ataaggttcc agctgatagt gcagtccgcg taacacaccc
    19741 ttagttgatt tagaatggtt atcctgaaca aactcaacct ttcgtcctac agcctcttcg
    19801 aaaactttct gattgaagct ttccataaaa aagccccgct catcaccaaa tacttttggt
    19861 tcaaaaatta gcacatcagg aatttcagtt ttaattacgt tcattttatt aataaccttt
    19921 aatcattttc agcagatact gaccataagc atttttcttc agcggctcgg ctaatacctt
    19981 cacttgctca gcatcaataa agcctttacg gtgagcaatt tcttccgggc aggaaacttt
    20041 cagcccctga cgctcttcaa ttgttgcaat gaagttgctt gcttcaataa gactttgatg
    20101 tgtccccgtg tccagccatg cataaccacg ccccatcata gcgacagaca aacgtccttg
    20161 gtccatataa atacggttaa tatcggtaat ttccagttcg ccacgtgcag atggtttaag
    20221 gtttttcgcc atttctacaa cgtcattgtc atagaaataa agcccagtaa ccgcatagtt
    20281 actttttggt tcctgcggtt tttcttccag actaattgcc gtaccgttat catcaaattc
    20341 caccacaccg tagcgctcag gatcatttac gtgataggca aaaaccgttg cgccactctc
    20401 tctgttaaca gcggtatcca ttaatttcgg caggtcgtga ccatagaaga tattatcacc
    20461 aagtaccagc gcacaatcat caccacctat aaattcttca ccaatgataa atgcttgcgc
    20521 cagaccatcc ggactcggtt gtactttata ttgcagatta agcccccact ggcttccatc
    20581 accaagcaat tgttgaaaac gtggtgtatc ctgcggcgta ctgataatca gaatatctcg
    20641 aatacccgcc aacataagcg ttgaaagcgg ataataaatc atcggtttat cataaatcgg
    20701 tagcagttgt ttgctcacag ccattgtcac aggataaaga cgagtgccgg aaccaccagc
    20761 cagaataata cctttacgcg ttttcatttc accattcctt ttaattcatc tcgctctagc
    20821 atcatgagcg agatgcaata atttgttaaa ttgccgtggt cgtaaataat tcgttgagca
    20881 tacgtttcac gcccacctgc cagtcaggca agacaagcgc aaagttctgc tgaaattttt
    20941 cggtattgag gcgagaatta tggggacgac gtgctggtgt gggataggcc gttgtcggca
    21001 cggcattaag tttgttaaga gcaaggttga tccccgctct gcgcgcttct tcaaatacca
    21061 gagcggcata atcgtgccag gttgttgtac caccagcaac cagatggtac aaaccagcaa
    21121 cttctggttt atttgcggcc acgcgaatgg catgagcggt gcaatcagcc agtaattcag
    21181 cccccgttgg tgcgccgaat tgatcgttta ttacagccaa ctcttcgcgt tcttttgcca
    21241 ggcgcaacat cgttttggca aagttatttc ctttaccagc gtatacccag ctggtacgga
    21301 aaataagatg ctttgcgcaa tgttcttgta acgctttttc cccagctagc ttggtttcac
    21361 cgtagacatt tagcggtgct gttgcatccg tttccagcca tggcgtgtca ccgttgcctg
    21421 ggaaaacata atcagtggag tagtgtataa cccaggctcc gacttcatta gctgcttttg
    21481 caatcgcttc gacgcatgtc gcgttaagta attgcgcgaa attcggttct gattccgctt
    21541 tgtctactgc agtgtgagca gcagcattaa caataacatc ggggcgaatt tttttgacgg
    21601 tttcagccac accttcgggg ttgctgaaat ctccacaata atcagtggag tgaacatcga
    21661 gcgcaatcaa gttacccaga ggagccagag cacgctgcag ttcccaaccc acctgccctg
    21721 ttttgccgaa aagcaggata ttcattactg acggccctca tagttttgtt caatccacga
    21781 ctgataagta ccgcttttca cattctcaac ccaatttgta ttagccagat accattccac
    21841 cgttttacga atcccgctct caaacgtttc ttgcggtttc cagcccaatt cgcggctaat
    21901 tttatcggca tcaatggcgt aacggcggtc atgccccggg cgatcggcaa cataagtaat
    21961 ttgctcacga taagatttct ctttcggtac aatctcatcc aacaaatcac aaatagtgag
    22021 cactacgtcg atgtttttct tttcgttatg tccaccaatg ttataagttt cacccgcttt
    22081 accttcggtt acgacggtat ataacgcacg agcatgatcc tctacataca accagtcgcg
    22141 aatttgatcc cctttaccat aaataggtaa tgccttacct tccagagcat tcagaataac
    22201 caatggaata agtttttccg ggaagtgata aggaccataa ttgttcgagc aattagtcac
    22261 aatggttggt aaaccataag tccgcttcca ggcacgtacc aaatgatcgc tggaagcttt
    22321 agacgcagaa tacgggctac ttggcgcgta agctgtcgtt tccgtaaata gcggtaacgc
    22381 ttcgttatta tttgcttcat ccggatgggg taagtcacca taaacttcat cagtagaaat
    22441 atgatgaaag cggaaacttt ttttcttttc atcattcaga gcagaccagt aattgcgcgc
    22501 cgcttctaaa agaacataag tccccacaat attggtttca ataaatgccg cagggcccgt
    22561 tattgaacgg tcaacgtggc tctctgccgc caggtgcatc accgcgtctg gctggtgctg
    22621 tgcgaaaata cgcgacatgg ctacagcatc gcagatatct gcatgctcaa aagcataacg
    22681 ttcagaatca gaaacatcag cgagtgattc caggttcccg gcgtatgtta atttatcgac
    22741 attaacaaca ctatcttgcg tattatttat tatgtgacga acaacagcag aaccaataaa
    22801 ccctgcgcca ccagtaacaa gtatcttcac ttttttattc catatagcta tagagcatgc
    22861 tgtgaaatag actgctccag atttgattaa tagaggtatt aatacacgct accgcccctg
    22921 gcttaacagc taccagtgca ctgcatgcat ctcaacgatg tggcgagcgt tagccactcg
    22981 cgccaaatcc gaaaaattca aacgttaatt gtcttaccaa acagccgtag aaacaaggaa
    23041 aatcctggaa aaatttgaat aatatgagct atgtaacttc ttgtttttat aggctttata
    23101 aaaacggcaa cggcaggaaa attcaatgag caaaacttat gccacgaatc ttcactgccg
    23161 ttataatttt cttatcaacc gttacatccg gtcagatttt cattattcgc ttaacagctt
    23221 ctcaatacct ttacggaact tcgccccttc tttcaggttg cgtaggccat acttcacaaa
    23281 cgcctgcata tagcccattt ttttgccgca gtcgtaactg tcgccggtca tcagcattgc
    23341 atcaacggat tgttttttcg ccagctcggc aatagcatca gtcagctgaa tacgtcccca
    23401 tgcaccaggc tgagtacgtt ccagttccgg ccaaatatcg gcagaaagca catagcgacc
    23461 tacggccatg atgtctgagt ccagcgtctg cggctgatcc ggtttttcga taaattcaac
    23521 aatgcggctg actttaccct cacggtccag cggctcttta gtctggatga cggagtattc
    23581 agagaggtca cccggcatac gttttgccag cacctggctg cggcccgttt cgttgaaacg
    23641 tgcaatcatg gcagcaaggt tgtaacgtag cgggtcggcg ctggcatcgt cgatcacaac
    23701 gtctggcagt accacgacaa atgggttgtc accaatggca ggtcgcgcac acaaaatgga
    23761 gtggcctaaa cctaaaggtt cgccctgacg cacgttcata atgatcacgc ccggcggaca
    23821 gatggactgt acttccgcca gcagttgacg cttcacgcgc tgctcaagga gtgattctaa
    23881 ctcataagag gtgtcgaagt ggttttcgac cgcgttcttg gacgcgtgag ttaccaggag
    23941 gatttctttg atccctgcag ccacaatctc gtcaacaatg tactgaatca ttggcttgtc
    24001 gacgattggt agcatctctt tgggtatcgc cttagtggca ggcaacatat gcatcccgag
    24061 acccgctaca ggaataactg cttttaaatt cgtcattatt tcatccacct gtaaaatggt
    24121 tgctgaatta tagcttgttc gatttttttc gccagcatca attaccctga attgattact
    24181 gaattacttg tgatgttacg ccgcttcgtt gtggattgca gtagcattgt tcctaagtat
    24241 ggctccattt ttccaggaat ggtcgcaaat ctactccctc cgttccggca atctaaagtt
    24301 aatcttttcc acattaacaa tatggtgatt aatcctgtcg atatcgacgg agctttgccc
    24361 tctttcattc accgcatgaa catttgcaag agacagcagt gtttcttttt tcgccataaa
    24421 gacgccgcga acgtctttgc gcatgtcgaa gttcatgctc aatgctggtc caactgagga
    24481 ttcctgcatc acattgatat ttcgcataaa aagatgttgc ggcttgttgt gtaactccag
    24541 cgaggcacgc ttcatctcaa tgttagttag cgacacaaag gagacggcat tcccggcgga
    24601 gatttggatg ccgcgcaatt tataagcaag atgggtatta tccagttgaa tattattcac
    24661 tcggaaattt tgcggtatcg agagatattt gcctttaatt accccatagc cgattaacat
    24721 cccggcgcta ttaatcattt caatattatc aatcacgaaa ttgtcacaac cgtaaatagc
    24781 gactgtcgcg ttatcaatgc ccgctttctt actgaaatcc ggcgtgatat tgcgggcatt
    24841 gatattacga ataacaaaat gtttaccatt ttcaacatgg atcaactgcc gacaatccga
    24901 tcccgtgata ttcgccacga caaagttttt cactgcctgg tcttccgggt agttgttatc
    24961 ataagtgctt cccgcaaggc ctatgccgat gccccagttg attttgccgt tggtacagtt
    25021 gatacgctcg atgacatggt cagatatcaa aatatcactg tcgttaattg ccacgttcca
    25081 ttcaatggcg tcgccctgta agtcgctgaa cttacagttg gtaatgttgg caccgataat
    25141 ctggttatga aatccctggc gtaagatggc gtaattagcg tggctgacag tgaggttatc
    25201 gatggtcagg ttgcgcatga cccgtttgtt tttgccgcca atataaatct gcattaccgg
    25261 gccaaagccg ctcatcgcca gccctttgat ggtgcagtca gaaccacgca catccagggt
    25321 aatgttatgc ataccgccgc cctctccccc tgtcacctgg ctgccgtcct gtaagacaaa
    25381 tcgccctctg ccgttgccgc gcaggcttcc aaggatgtgt aacgttttgc cgggagggat
    25441 gaagatgccg gtgttgatat tgtcacaaac gaatccggca ggcacgacga ccgtttgccc
    25501 ttcgctgaag gcttgtttaa atgaggcgat ccagtcatgt gggttgtagt cgttaatgtt
    25561 aacgctttgt cgggcgggaa gtgcgcgagc gaaaggggta tggaggaagg caagcgccga
    25621 gcttgccgtc aggaaggtgc gtcgggagag tgttttaaat ggcatacgtt ctcctctata
    25681 aagcctgcag caagctggcg agttctcgat taatcacctg ctggttaaaa tcgtgttcga
    25741 ctttttcgca cgcacgtttg acgaccggag ccagttcgtc ggtatccagt tggctaaacg
    25801 ccgccagacg ttgtgccagt gcgcgagcat cgttctcagg cagcagccag ccggatttat
    25861 cggcctccac cagttcagga attccgctat gcagagtaga aaccaccgga ataccgaccg
    25921 ccatcgcttc cattagcgcc accggaatgc cttccatatc gccatccgcg cccgttaccg
    25981 atggcaacag gaagacatcc gcgtcgtcga gcatctcctt cacttcatgg ctcggtttaa
    26041 agcccggcat ctctaccaca tcttccagtt gatattgttc gatgatggtg cgcaggcgtc
    26101 gttcccacag gccaatgccg aggatgcgat agcgaaatgc cacgccctgc tctttcaact
    26161 gacggcaggc ttcaatcgcc acatgcaggc cttttttctc ggttaagcgt gcgacagata
    26221 tgatttccag cggcgttgcg ggcgctttca ccggacgcgg gctaaagcgc gtcatgtcca
    26281 cgcccatacg cgatacggcg attttttctc tggggcagcc cattttttgc agccttccgg
    26341 cccacagatc gcttatcggt aacatcaggt cgccacggcg aaacagttgt tgatattcgg
    26401 gagtgtagtg gttgagcact tcccgactgg agatatcaat accgtggaag atggtggcaa
    26461 ttttgccgcg aatgataccc agttcgcgta gttttgctgc ggttaccccc gccgggccaa
    26521 aatgagcgat gaacacatcg gcgcgaaacg gtgttgcgac ctgaccacaa atggcagaca
    26581 aaatcaggtt ccgcgactcg gcaccatagc gtttgaggtt gagcgcatgc caggtatttt
    26641 tacgatgaat gccacgtaag gtctggctgg cgcggtggcg cagtttcgct actttgcccg
    26701 caggttcgtc ctgtaaccag cgggttctgg cgacaaggtt gtatttcgtc catgccgcgt
    26761 gggtgttctc tgtatcgcct ttttgcaaag cgacaatctc cacttcaaag cccatatcaa
    26821 taaacgcggt aatttgattg aggacgaagg tttccgacga cagcgggaat ttcaataaga
    26881 agaagccgac cttcatttca cctccccgat gcgctcaagc acagactgca ccatctgcat
    26941 tcccgtctga cgttcgcgac tgacggcttc gttaaatcga gcgttcagca ccggaagctg
    27001 gcctaaggta tccgcaacca tcgcttgcag gctgccgtcc agtaaatgac gaatatcaat
    27061 cgccatctct ggcagtccca gctgctgcat aatcccggcg gatttatgtt catagttgat
    27121 ggcaatcgcc ggagtagcaa agttcattga gataatggcg gagtgcaggc gcgtaccgac
    27181 ggtgagttca caggctccga gaattttgcc catttccaga tcgttgagtt catccatcac
    27241 tacgtggtaa cgggcaggat cgctgatgtg ctggcgcaga ttgagcgcca ccatgcggtc
    27301 gtctttgtta tagctgtcga tacccgtaca ggtggagagc gcaatcacct gatagccttc
    27361 atcgagaatg cgattgacca ccccggcaaa ggctttttca tacgcttgtt gagtggtgcc
    27421 gagacgtttg tcgaacggtg ccagttcgcg cagggtaatt gccactgttt tctgttgtgc
    27481 ggcaacgtcc agccagtgtt gaacggcata gctggcggtg aagtcttcgg tgtggtgatc
    27541 gaccagccac gcggtatcga cgccatgttc cacttttgcg gtggtgatat ttctgcgttt
    27601 catcaaatca aggctgaccg attcgcgcag gatcagcgcg tcgcagtgac caaagacata
    27661 gttcgccagt tggttaaatt gctcatcctg gaaggggccg acgctgtgac caatcataaa
    27721 cagcggcttt ttcgccataa atgtgcaaag cgcatgttca aactgcggca cgccgtagag
    27781 atcgacaaaa aacgatccgc cgacctgaat aatggcgtcg taacctgaca gcaggcgcac
    27841 gaagtcggtg aatccctggg cgatggcgat attgcgcagc ttgccagtgt cggtgacgcg
    27901 tgagagcaat acctgatgct ggtagcggcg acggaggact tttttaatgc gcccgacaac
    27961 gcccgccgcg ctattgtgtt gtgtcatctg caggaacagc ggatcgccca ttaccgggcg
    28021 gttgagcagc caggaagaac tgaccggata gcggctcatc acgtccactt cggtatgtgg
    28081 attgaggatg ttgatggcat caagtaagcc gcgcaagatg gcgctgtcgc cacgattgcc
    28141 gcaagtgtgg ttgcccagaa taagtaattt cattttttcc tcataaattt gatgccaggt
    28201 gaggctgtgt ttgttgtgtt cgttgccgtt ttttgcctga tgcgacgctg gcgcgtctta
    28261 tcaggcctac aggtcccgag cacagaaccg taggacggat aaggcgtttt acgccgcatc
    28321 cggcaaccgt tgtcggaacc gaaaacagca attcaccccg cccgtaaaag cattttcatt
    28381 ttttcgctgc gacaaaactg acgcttcact tccaccacca gcggatggcg tgacagcaca
    28441 atcatcacca caaacgccag cacgcccgcg gctatttgca ccgccagcag catccccagc
    28501 gccagttgcc ctttcagcac aatgcccagc gcataactga ccaccagcgt cggcaacgag
    28561 agataaaacg gcagccacaa actcaggatg tactggcgat aactggaacc aagaaccggt
    28621 ttaatcatca cgaagtaact cagaatggtg ttgataatct gcaccagcag gaaaccgagc
    28681 gtgacgccga ttgcgcccgc catctgccca ccaataacaa tcgccggaat aaacagaaat
    28741 gttttgaata cgttgaattt aaagctgata tcgacccgcg ctttcgccat cagcagcgaa
    28801 ccaatcgggt tacccacgga gcgcagcaga cccacaatac acagcaattg cagcaccgga
    28861 ataatgctgt tccacttttc gccgaatacc agcggtacga agttattcga caccaccatc
    28921 agccccagca gcgccggaaa gttgataatc cccactatcg acagcagctt gtagaagtta
    28981 acacgcagct tttcggtgtc gtcctgaatt ttggcaaacg ccggaaacaa cacgcgggtg
    29041 atgattgggt tcagcttcat cggcggtacg acggccacgt tgtacgccag gttgtatccc
    29101 cctgccacgc ccgcgccaag aatacgcgcc agcaccagcg ttgaaaggtt ggtgttgaga
    29161 tagttgatga tgctgtccgc cgtcagccag gcaccaaaac gtaagttcgg tgccacagac
    29221 gccagcgaga agtgcagacc ggggcaataa attttgcgtc caaagtagcc aaacagcagt
    29281 gttctcaccg cactattgac cagataaccg aggatcgcgg tcatcgccag cggccagaaa
    29341 tgggcgctaa ctaccgtaaa agtgaagccc gccagcaccg cgctggtttc gatcatgccg
    29401 attttgttga actccagctc cttctgcatc aacgcgcgga actgttgccc gtgggggatc
    29461 accacaaacg ccagcgacaa tgttttaatc aacggtgcca ggtccgggtt attcagcacg
    29521 tcgccgatga catcactcaa caaaaacacc gccacacata ccacaagccc cagcccgacg
    29581 ttcagccagt acagcgtggt gagttcaagg tgactgattt cttttcgctg aataatcgag
    29641 ttagcgatac cgaagtcaga aagcgtatct gccagcgcga taatcaccag cgacacagtc
    29701 agcaggccga actggtggtt gtcgataatc cgcgccagca cggtcatctg caccagcccg
    29761 aggccgatga tgatcaccgt ggcaatagcc gaccacttcg cgccgctgat ggttttttca
    29821 cgtaagctca tatcaatatg ccgctttgtt aacgaagccc ttgaataccg tcaggaaaac
    29881 gattttgata tcgaaccaga cgctccattc gcggatgtac tcaaggtcga actcgacgcg
    29941 tttttccatt ttctccagcg tgtcggtttc gccgcgccag ccattaatct gcgcccagcc
    30001 ggtaatgccc ggtttcactt tatggcgcag catgtagcct tcaatgagct ggcgatactg
    30061 ttcgttatgc gctactgcgt gcggacgtgg accgacaatc gacatccccc cggtcagcac
    30121 attgataaac tgcggcaatt cgtccagcga ggtgcggcgc agaaagttcc ccactttagt
    30181 gacgcgcgga tcgttctgcg tcgcctgggt caccactttg tcgttctcca tcactttcat
    30241 ggaacggaac ttccacactt tgatcggctt gccatccata ccatagcgag tctggcggaa
    30301 aataaccggc cctggtgaac tgagtttcac cgccagcgca atacagcaca gcaccgggga
    30361 gatcagcagc agaataagcg tcgccagcac aatgtcttcc gcacgtttta gcaggcggtt
    30421 aacccccgaa agcggcgtgt catacagcgg caccaccgga acgccgttca tctcttcgag
    30481 acgtgaatgg agaatgttga aggtaaagac gtcggggatc agcagcaccg aacaggtggt
    30541 atccgccagt tgatggacca gttttttcac tcgtgcgccg tcgcacattt gcatcgcgat
    30601 atagacgtta tgaatcttgc ccgcttttgc atcctcgacc agctgttgca ggttacccgc
    30661 ccagtcgtta gaaacgccgc ccggtttcgg gtcgtggtaa acgcccacca cttcaaaccc
    30721 taaccacggc tgattacgga aactctccat cagcatttgt ccggcggcta aatcccccgc
    30781 tacggcgacc atccgcttgt tgtagccatg attacgcagc cagcccgccc caatgcgaat
    30841 acatgaacgg caaaccacca gtccgatgct ggtcagccca taccacgcca gccagatttt
    30901 cagttgcgtg tcgaaatcat tgttgaacgc caccagtccg gcgctgaaaa tcacgcttaa
    30961 ggtccagttt tgtagcaaca gggcaaattc tgtcgctgcc cgaacaccgc gccatgagcg
    31021 ataaaaatcg gtgatgccgc ccagcatctg gaacaccacc agcgtaatca gcgccaccaa
    31081 caggtgcatg tagaggaatg acagtccgct gacttcgcaa accagccata gcccggcaaa
    31141 catgatggtg atatctgaaa agcgttgcac catagagatt aacgatgcat tggttttcgc
    31201 tcgctcgcgc ttttttagat ttgtcatcgt tgttcctgtt attagcccct tacccgcagt
    31261 ggggtaaggg aagatccgac attactcgtt cagcaacgtc agcagagttc gcgttcgcgc
    31321 ttccatcagc ggcacatcac cgcgcgattc cacattcagg cgcaccaccg gttcggtatt
    31381 ggaggtgcgc aggttaaagc gccagtcggc aaaggtcatg ctgatgccat cgctgcgatc
    31441 caccgccagc gcctcacggc taaaatgctg ttccacgcgg ttaatcgcct caacgggttg
    31501 cgccagtttg ctgttgatct caccgcttgc cggaaacgcc gccatccggt cgcgcaccag
    31561 ttcgcccagc gtttttcctt tcaggcacac cagttcggcg accagcagcc acgggatcat
    31621 gccgctgtcg cagtaagcga aatcacggaa gtaatggtgg gcgctcattt cgccaccgta
    31681 gatggcgtct tccttgcgca tccgttcttt aataaaggcg tgtccggttt tcgacattac
    31741 cggcgtgccg cctgcagcag tcaccacatc aatagtgttc caagagagac gcggatcatg
    31801 gatgatcttc gcgccgggat ttttttcgag gaacgcttct gccagcaggc cgacaatgta
    31861 gtagccctcg ataaactgcc ctttttcgtc aaacagaaaa cagcggtcaa aatcgccatc
    31921 aaaggcaatg cccatatccg cgccgtgttt gatgaccgca ttgcgggtgt cgtcgcgaca
    31981 ttccggcagc agcgggttag gaataccgtt ggggaaattg ccgtccggtg tgttgtgtac
    32041 tttgattaat tccacgggcg cgccgagggc tttaaagcgg gcttcaatgg cgtccaccac
    32101 cggacccgct gcgccattcc cggagttgat caccagcttg agcggcgtga gatttttgac
    32161 attgatataa ccgaacaggt gatcaacgta agcgtcacgc aggttgattt gctgatagcg
    32221 accgcgtttg gtttcatcga cgggaggaaa gtcgttggct tcagccagac gctggacgtc
    32281 gcgcagtccg gtatcaccgc tgatcgtgcg agccccctcg cgcaccagct tcatgccgtt
    32341 gtaatccatc ggattatggc tggcagtaac ttcaatgccg ccatccacgc cgagatggaa
    32401 cgtggcgaaa tagatctctt cggtgccgga cataccgata tccagcacat cgacgcccgc
    32461 atcctgtaaa ccttttgcca gcgccagttt taaggtttcg ctggtgagac ggacatcacc
    32521 gcctaacaca atggttttcg gtttgagaaa ttcgccatag gcgcgaccaa tgcgccaggc
    32581 gatatcttca ttcagttctt cgcctaattt cccgcgaata tcataggctt taaagcaggt
    32641 taattttttc atgtcgttac cctttttcag gcaatagttg gccctgaccg aaacggccaa
    32701 ttttattttt gtcgttattt acaccgcggt ttcgcattca ttgcctgatg cgacgctgcc
    32761 gcgtcttatc aggcctacaa aaccgaaccg taggtcggat aaggcgttca cgccgcatcc
    32821 gacaaacagc gcctgatgcg acgtttacac ccgtccgtag cgatccgcga agcgcaccac
    32881 atcatcctct tcgagataag agccggagcg cacttcaatt aaatcgagcg gaattttccc
    32941 cgggttttcc aggcagtgcg tcgcccccag cggaatataa atggactcgt tttcaccaag
    33001 cagtttgata tcaccatcaa tggtgacttt tgccgttccc gcgaccacta cccagtgttc
    33061 cgcgcggtga tggtgcatct gtaccgacaa accctcgccc ggtttcacgg tgatgcgttt
    33121 cacctggtag cggtcgcccg cgtcgataga gtcatatttg ccccacggac gatacacttc
    33181 gcgatgcacc cgatgctcat ggcgaccatc ggctttgatc tgctcgacca cttttttcac
    33241 atcctgtacc gcgttacggt cggcaatcag caccgcatct ttggtctgca ccactaccag
    33301 atctttcacc ccgacggtgg tgaccaggcc tgattcggca tacacatagc tgttttcagt
    33361 tttgtgatta atcacatcgc cgtggcaaac gttgccctcg gcggtgtggg cgctgatctc
    33421 ccataatgaa gaccaggaac caacatcgct ccagcccgca tccatcggca ccacaacagc
    33481 atctgccgta ggttccatga ccgcgtaatc caccgactct tccgggcagg cgagaaacgc
    33541 ttcttcatcc acacgaataa aatcgagatc cggatcgacg gcgctcatcg ctttttcgca
    33601 ggcatcgagg atatccgggc gatatttttt cagttcttcg agatagcgtc cggcgcggaa
    33661 caggaacatg ccgctgttcc agtaatattc gccgcttgct acataggcct gggcggtttc
    33721 cagattcggt ttttcgacaa actgcgccac ttcaaaggcc accgtatcct gctcacccac
    33781 agacacttca ccgcgacgaa tatagccata accggtttct ggtagatccg gcacaatgcc
    33841 aaaggtcacc agcttgcccg cttcggcata aggcatggca ttacgcacgg cggcacggaa
    33901 cgcgtcttca tcggcaatca catgatccgc cgccaatacc agcattaacg ggtcgctctc
    33961 cgggctatga cgttttgccg ccagcgccgc cagtgcaatg gcaggtgcag tgttacgccc
    34021 tgccggttcg agaataatgt tctcggtgag tttgttcagt tgacgcagct gttccgcgac
    34081 aataaagcgg tgctgctcat tgcaaatcac caccgggctt tcgcactcca cgccgttcag
    34141 gcggcagatg gtggtttgca gcatggtgag atcgcctttc aggcataaaa actgcttggg
    34201 gtaaagtacg cgggaaagcg gccataaacg gctaccggag ccacctgcca tcacaactgg
    34261 atagagtttc gactgcgcca taattatccc cgaatatcat ttataaattg acgtaacacg
    34321 ttctctttat cgagcgtgcg ttcggcatat tcacgtgcca ccgtgttatg tttgggcagc
    34381 aggagcgcct gacgaatccc cgccaccagc gcctcgaccg attccggttc tacgcaaacc
    34441 gcaatacccg gaaaggtttc gcaaagctgc cccagttctg tgtgggcctc tgcggtaatc
    34501 accgcgttac cgcctaccgc cagaatattg gtcagtttcg acggcaatac ggcatctgcc
    34561 gcgccgcgtt tttgcaccac cagatggcaa tcgcccatct tcagcagtgc aggtaaagcg
    34621 tcatacgatt gcagcgggaa aaattgcatg ttgcgcagtc cacgctgctg cgccattttt
    34681 tccagccgcg ctttgccgcc gccctgcccg acaatggcaa aaatcagcgg ttcatcgcgc
    34741 aggagatcgg cagcttcaat aacgttttcc agcccctgct tttcaccaat attgccggag
    34801 taaagaatga tttttttgtt atccggcagg ccaagctggt tacgaagggc atcaacatcg
    34861 gcgtctgcaa catgctgaaa acgggcaatt tccgaccagt tggggaagaa gatgacgtta
    34921 tccgccgcca cgcctttttc gatggcttta ttcatcatcg aacgcgaaat cgtggaaacg
    34981 ttatcgacgt tatgcagtcc gctacgttcg aacgccgttg ccagctgtgc cactttgccg
    35041 cctttgcctt ttccggcaag gcccagcccc agcatggcgt ccacttcgta atcctgaata
    35101 tgcagcacgg tacgcgcacc agagagcttc gccagcaggc gcattcccgg cgtgcaaaac
    35161 agcgttggca ctacgccaat aatgcgatcc ggcttccagc gacgttgcgc catcagtgga
    35221 aagaaactgc tgacggcaaa actgccgaga tgcaacaagc gtttcagggt gctcggctgt
    35281 tttggcacgt acagcgggca gcgccacacc gtggcggccc cttcttctcg tttgtagcgc
    35341 caggcggaat agttctcacc cacctgccat tgcgggtagt aaggcggtgc ggtaatgacc
    35401 cgcacctcat gaccttgtgc cgccaaccat tccaccatct cgccggtgta tttgccgatg
    35461 ccggttaact ccggcgagta gttaatgccg tagaccagta ttttcataat ccgggtactc
    35521 cggcacgctt ctcagcgaga aaataggctc ggctgttagc gtgaacatcg ttactggcga
    35581 gcagcgcgtc cggcgtcagc cagcggtaat cgtcatgctg ctcatccggc agtaacagct
    35641 cttcttccgc tactctgaag cgaaaaccga gcaccacata gtgagtggtg aaatccgtgc
    35701 cggagaagtt atcgtcataa aaatgctgcc agacaccgta aaactggcct gctgttatcg
    35761 gcagacgcag ccccagttcc gccatcgtca gccgctcaaa tgcggcttcc agcgtttcgt
    35821 ctttctgcac gcgccctccc ggcacaaacc agtaaccctg cgccgggcgg ttggttcttt
    35881 tgccaagcag aaactcgccg cgactgttct cgacaataaa gtcgagagag acaagcggtg
    35941 tggagcgcac tacagtggca aagtcttcct gacgtaaaaa catcattccc cccgaaagcg
    36001 gtcttgattc tcaaggaacc actggtaagt gctggcaagc cccgcttcca gtgagatttc
    36061 gtgataccag ccaagctgat gcaggcgcgt cacatccagc agtttgcgcg gcgtaccatc
    36121 cggtttgctg gcatcaaaaa ccacccgacc tttgtaaccc accactttgg cgatggtttg
    36181 cgccagctcg cggatagtgc agtcaacgcc cgtgccgacg ttaatgtgcg acagcatcgg
    36241 ttgggtattc tccagccaga cttcatgcgc cagctccatg acatgaatgc tcgccgccgc
    36301 catatcatcg acgtgcagga attcacgcat cggtgtaccg ctgccccata ccaccacgtc
    36361 cggcgcattc tgtgccgtcg cctcgtggaa gcgacgcagc aatgctggta tcacatggga
    36421 gttactcggg tggaagttgt catgcggccc gtacaggttg gtcggcatga cagtgcggta
    36481 atcgcgtcca tactggcggt tgtaagattc gcacagtttg atcccggcga ttttggcaat
    36541 ggcgtaaggc tcgttggtcg gctccagcgt gccctgcaat aactcgcttt ctgccatcgg
    36601 ctgttttgcc agtttcgggt agatgcagga cgatccgaga aacatcagtt tgttcacgtc
    36661 gttctgatgc gcggcgtgaa tgatgttgct ctcaatcatc atgttctggt agatgaaatc
    36721 cgccggatag gtgttgttgg caacaatgcc gcccactttc gccgccgcca gatagacctg
    36781 gtcaatgcgt tcgctggcaa agaaatcatg cacggcgcgg ctgtccagca ggttcagttc
    36841 atcgcgggta cgtaatacca gctccacatc accgcgctgt tcgagctgcc gcttgatggc
    36901 agaaccgacc atcccacaat ggccagcgat aaaaatgcgt tgtttactca tgcttatgac
    36961 tccagcgcga tcgccacgtc gtagccgtga gatttcagca gagagtgttt tttcgccgct
    37021 tcgaggtcat tagccaccat ttcagacacc atctctctga gggtgatttc cggtttccag
    37081 cccagttttt cgtgcgcttt ggtcgggtcg ccgagcagcg tttcaacttc tgccggacgg
    37141 aagtaacgcg gatcaacggc gataatcaca tcacccggtt taacgcccgg cgcgtcatgc
    37201 ccggtgacgg aaaccacaat gcccttctct tcaacacccg tgccttcaaa gcgcagtttg
    37261 atgcccagct gtgctgccgc catttccaca aactgacgta cggagtactg aacgccggtc
    37321 gcgataacga aatcttccgg ctgttcctgt tgcagcatca tccactgcat ttttacgtag
    37381 tctttggcgt ggccccagtc acgcagggaa tccatattgc cgaggtacag gcacgactcc
    37441 agcccctggg cgatgttggc gattgcgcgg gtgattttgc gggtaacgaa ggtttcgccg
    37501 cggcgcgggg attcatggtt gaagagaatt ccgttacaag cgtacatgcc gtaggattcg
    37561 cggtagttaa cggtgatcca gtaggcgtac agtttggcga ccgcatacgg agatcgcggg
    37621 tagaacggcg tggtttcttt ctgcggaatt tcctgcacca gaccatacag ttcagaggtg
    37681 gaagcctgat agaaacgagt tttcttttcc agaccgagga agcggatcgc ctccagcagg
    37741 cgcagcgtac ccatcgcgtc gacgtcagcg gtatattctg gtgactcaaa agagaccgca
    37801 acgtggctca ttgcgcctag gttgtacact tcatccggct gtacttcacg caaaatgcgc
    37861 gtcaggttag aggtatcact caggtcgcca taatgcagat ggaatttcgg gttgcaggtg
    37921 tgcggatcct gataaatgtg atccacgcgc tcggtgttga atgacgatgc gcgacgctta
    37981 ataccatgca cctcgtaacc tttttccagc agaaactctg ccaggtaaga accgtcttgt
    38041 ccggttacac cggtgatgag agcgactttt gacatgtctt attcctctgt atttttgaat
    38101 ttattcagtt tcaacgcgtt cgcgtatcac cactgcggga ttcccccggc aaaccacatt
    38161 tgccggaagc gatttaaaaa cactgcttcg tgcacccacg acggtgccgt cgccgattgt
    38221 gacgcctggg gcaacaaaga catcggttgc cagccagcat ttctcgccaa tcacaatagg
    38281 cgtggcgtta atggtgaaat gttgacttgc atggtcgtgg ctaccggtgc ataaataact
    38341 tttttgcgat atcaccgaat gtgcgccaat ggttatttca ccgagggtat ataaattgac
    38401 gtcatcgcca acccacgcgt aatcacctaa ggttaatttc cacggatagg taatttttac
    38461 tgacggacga ataactacgt tttttcctat ttttgctccg aataaacgta ataaaaaagc
    38521 ccgccagcga tacaatactt gtggcgacca ggcaaatatt gttgcctgta ctgcccacca
    38581 taattgcact ttaatagcgt tgccgccccg gaaccctttc ggcaccgaga aaccgcttaa
    38641 atcttgcatt ggttttcctt atattcagac tttgttatat aaggctttag tcttacctgt
    38701 cgtacgttgg cgtaaatgcc aggataattc tgcccagaag ccaggtacat gtaatatctg
    38761 ccgttggact tttttggcgt cagcac
//
LOCUS       DAJTSF010000028        38249 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000028 DAJTSF010000000
VERSION     DAJTSF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38249
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.028"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..73)
                     /locus_tag="OO335_001532"
     CDS             complement(<1..73)
                     /locus_tag="OO335_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCR6001971.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO335_001533"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO335_001533"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6001972.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="OO335_001534"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="OO335_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6001973.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO335_001535"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO335_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6001974.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="OO335_001536"
     CDS             2703..2939
                     /locus_tag="OO335_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6001975.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="OO335_001537"
     CDS             2942..3799
                     /locus_tag="OO335_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6001976.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5811
                     /locus_tag="OO335_001538"
                     /pseudo
     CDS             3799..5811
                     /locus_tag="OO335_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="OO335_001539"
     CDS             complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="OO335_001539"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR6001977.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6414..7310)
                     /locus_tag="OO335_001540"
     CDS             complement(6414..7310)
                     /locus_tag="OO335_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6001978.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7359..7598)
                     /locus_tag="OO335_001541"
     CDS             complement(7359..7598)
                     /locus_tag="OO335_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6001979.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7701..8300
                     /gene="nemR"
                     /locus_tag="OO335_001542"
     CDS             7701..8300
                     /gene="nemR"
                     /locus_tag="OO335_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6001980.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8337..9434
                     /gene="nemA"
                     /locus_tag="OO335_001543"
     CDS             8337..9434
                     /gene="nemA"
                     /locus_tag="OO335_001543"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR6001981.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9515..9922
                     /gene="gloA"
                     /locus_tag="OO335_001544"
     CDS             9515..9922
                     /gene="gloA"
                     /locus_tag="OO335_001544"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6001982.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10025..10672
                     /gene="rnt"
                     /locus_tag="OO335_001545"
     CDS             10025..10672
                     /gene="rnt"
                     /locus_tag="OO335_001545"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6001983.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10765..12879
                     /locus_tag="OO335_001546"
     CDS             10765..12879
                     /locus_tag="OO335_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6001984.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12889..14181
                     /locus_tag="OO335_001547"
     CDS             12889..14181
                     /locus_tag="OO335_001547"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6001985.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            15131..15223
                     /locus_tag="OO335_001548"
     CDS             15131..15223
                     /locus_tag="OO335_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR6001986.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(15267..16475)
                     /locus_tag="OO335_001549"
     CDS             complement(15267..16475)
                     /locus_tag="OO335_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6001987.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16483..16983
                     /gene="tnpA"
                     /locus_tag="OO335_001550"
     CDS             16483..16983
                     /gene="tnpA"
                     /locus_tag="OO335_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR6001988.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(17022..17369)
                     /gene="grxD"
                     /locus_tag="OO335_001551"
     CDS             complement(17022..17369)
                     /gene="grxD"
                     /locus_tag="OO335_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6001989.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17704..18531
                     /gene="mepH"
                     /locus_tag="OO335_001552"
     CDS             17704..18531
                     /gene="mepH"
                     /locus_tag="OO335_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6001990.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18659..19240
                     /gene="sodB"
                     /locus_tag="OO335_001553"
     CDS             18659..19240
                     /gene="sodB"
                     /locus_tag="OO335_001553"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6001991.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19386..20555)
                     /locus_tag="OO335_001554"
     CDS             complement(19386..20555)
                     /locus_tag="OO335_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6001992.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20721..20810)
                     /gene="ynhF"
                     /locus_tag="OO335_001555"
     CDS             complement(20721..20810)
                     /gene="ynhF"
                     /locus_tag="OO335_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6001993.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            21109..22134
                     /gene="purR"
                     /locus_tag="OO335_001556"
     CDS             21109..22134
                     /gene="purR"
                     /locus_tag="OO335_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6001994.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(22131..23063)
                     /locus_tag="OO335_001557"
     CDS             complement(22131..23063)
                     /locus_tag="OO335_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6001995.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            23176..24387
                     /locus_tag="OO335_001558"
     CDS             23176..24387
                     /locus_tag="OO335_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6001996.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24678..25826
                     /gene="cfa"
                     /locus_tag="OO335_001559"
     CDS             24678..25826
                     /gene="cfa"
                     /locus_tag="OO335_001559"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6001997.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25866..26507)
                     /gene="ribE"
                     /locus_tag="OO335_001560"
     CDS             complement(25866..26507)
                     /gene="ribE"
                     /locus_tag="OO335_001560"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6001998.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26722..28095
                     /gene="mdtK"
                     /locus_tag="OO335_001561"
     CDS             26722..28095
                     /gene="mdtK"
                     /locus_tag="OO335_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6001999.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(28136..29392)
                     /locus_tag="OO335_001562"
     CDS             complement(28136..29392)
                     /locus_tag="OO335_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6002000.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29700..29776
                     /locus_tag="OO335_001563"
     tRNA            29700..29776
                     /locus_tag="OO335_001563"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29734..29736,aa:Val,seq:gac)
     gene            29781..29857
                     /locus_tag="OO335_001564"
     tRNA            29781..29857
                     /locus_tag="OO335_001564"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29815..29817,aa:Val,seq:gac)
     gene            29965..30270
                     /locus_tag="OO335_001565"
     CDS             29965..30270
                     /locus_tag="OO335_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6002001.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30396..31818
                     /locus_tag="OO335_001566"
                     /pseudo
     CDS             30396..31818
                     /locus_tag="OO335_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(31830..32642)
                     /locus_tag="OO335_001567"
     CDS             complement(31830..32642)
                     /locus_tag="OO335_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002002.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32646..33431)
                     /locus_tag="OO335_001568"
     CDS             complement(32646..33431)
                     /locus_tag="OO335_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR6002003.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33428..34096)
                     /locus_tag="OO335_001569"
     CDS             complement(33428..34096)
                     /locus_tag="OO335_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6002004.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(34160..34798)
                     /locus_tag="OO335_001570"
     CDS             complement(34160..34798)
                     /locus_tag="OO335_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR6002005.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34811..36912)
                     /locus_tag="OO335_001571"
                     /pseudo
     CDS             complement(34811..36912)
                     /locus_tag="OO335_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(36933..37559)
                     /locus_tag="OO335_001572"
     CDS             complement(36933..37559)
                     /locus_tag="OO335_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR6002006.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(38015..38224)
                     /gene="fumD"
                     /locus_tag="OO335_001573"
     CDS             complement(38015..38224)
                     /gene="fumD"
                     /locus_tag="OO335_001573"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR6002007.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         9208 a         9992 c         9366 g         9683 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccagctg cccaggaatg agcagtgaca gattggcgat gccgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gtaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga aggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tactttcgct attcagcttt gtggtcaaat ttggcctgat ggtccagatt
     5101 agcgatctgt ggcaattttt actgtttctc tttccactgc tggcgacaat gcagcttctt
     5161 aaattgcaga tgcccaaatt tgccgcactg tgggggcaac tgattgtttt tatgggttcg
     5221 tttatcgctg tcactaatcc cccggtgtat gattttgctg attttcttaa ctataatctg
     5281 gcaaaaatcg ttggcgttgc gctggcttgg ctagcgttcg ccattctgcg tccaggatcg
     5341 gatgctcgta aaagccgccg ccatattcgc gcgctgcgcc gggattttgt cgatcagcta
     5401 agccgccatc caacactgag tgaaagcgaa tttgaatcgc tcacttatca tcacgtcagt
     5461 cagttgagta acagccagga tgcgctggct cgccgttggt tattacgctg gggtgtagtg
     5521 ctgctgaact gttctcatgt tgtctggcaa ttgcgcgact gggaatcgcg ttccgatccg
     5581 ttatcgcgag tacgggataa ctgtatttca ctgttgcggg gagtgatgag tgagcgtggc
     5641 gttcagcaaa aatcactggc ggccacactt gaagaattac agcggatttg cgacagcctt
     5701 gcccgtcatc atcaacctgc cgcccgtgag ctggcggcaa ttgtctggcg gctgtactgc
     5761 tcgctttcgc aacttgagca agcaccaccg caaggtacgc tggcctcgta attacttaat
     5821 tacaccacag gcatagcgtt caccgccacc gcccagcggt ttaggttgat cggacatatt
     5881 atcgccgcca acgtggacca tcagcgcctt gtctttgatt tcatccagtg atttcagacg
     5941 aggcgcgatg acggcatcgg tagctttgcc gtcattattg acgaccagtg caggcagatc
     6001 gcctaaatgc ccggtacctt ctggcccttc atgtttaccg gtattttgtg gatcaagatg
     6061 cccgcctgcg gattccgcgg cgctggcttt gccatctttg gtggctggct ggcagcttcc
     6121 tttggcatga atatggaagc catgttcacc ggggggtaat gctttcagat cgggtgaaaa
     6181 ctccagacct ttatcggttt cagtaatggt gacgctacca attgactgcc caaccccttg
     6241 cgacgtgacg aggttcatct cgactttttc actggcagct tgtgcgccgg tcgcaacaac
     6301 cagcgccaga atggccagac taaaacgttt cataggacct ccgttcattg tgcattcctg
     6361 aataagtgta aaccagtgac acatttttga acggcggcta ttcctaaaag tgcttacggt
     6421 acgtcgtacc ccagtgcagc tttacggata cgaaaccatt gttgacgggt cattttcagt
     6481 gtttctgctt cgacagctgc ccgtacgcgc tcaattttac ctgagccgat aattggcagc
     6541 ggctgcgacg gtaaacgtaa tacccaggcg taaaccacct gttcaatcga gcccgcgttt
     6601 aactcctccg ccaccacagc cagttcatca cgcagcggct ggaaataatc atcattaaac
     6661 agacgaccac caccaaggca ggaccacgcc atcggacgaa cacgcagttg ttgtagttgg
     6721 tcgagcgtgc catccagcag taacggctga tgcaccgggg atatttccac ctgattagtg
     6781 gcaagggtaa acggcagacg tgattgcaac agggcaaatt gcgcaggcgt aaagttcgat
     6841 acgccaaaat gacgcacttt gccgctctga tgcagatgtt tgaacgcgtc cgccacttca
     6901 tcggcatcca ttaacgggtc tggtcggtgg attaacagca gatccagatg atcggtcgcg
     6961 agattaatta gcgactgttc ggcgctctta atgatgtgat tgcggtcagt gatgtaatga
     7021 ccaatgacgt tttcttcacg cgcggtcgtc gcgataccgc atttactgac gatttccatc
     7081 cgttcacgca ggtgaggtgc cagtttcagt gcctcgccaa acgccgcttc gcactgatag
     7141 ccaccataaa tatcagcatg gtccacggtg gtcacgccga gatccagatg ctcttcaata
     7201 aaactgacca gctggcgggc ggacatattc cagtccatca atcgccagta gcccatcaca
     7261 aaacgggaaa actccgggcc ttccggcgca atagtaatac gctgaaccat aatcgcttcc
     7321 tcttatcaga tataagagga gtatacgcaa gattaggttc aaaagagtga tggttgctcc
     7381 ggttcgtctg atgacgctgg cttatttgcg cgtaatttgc gcattaatcg ctgccgacaa
     7441 aggcgcagca cctcttgttt ttcgccatcg ctcattttat tccagttaaa acgctcatcc
     7501 cgactacgaa aacagccgcg acaaaacccg cgttcgtcag actggcaaat tccccggcac
     7561 gggctctgga cgggaaagaa ctctaattgc tccgccacct cgccctcctc agataagatt
     7621 attaccatta ttgaagctgt taatgtccaa agtagcaact ttgcttgcac tagaccaact
     7681 ggtctactac actccaacgc atgaacaaac acaccgaaca tgatactcgc gaacatctct
     7741 tggcgacggg cgagcaactt tgcctgcaac gtggattcac cgggatgggg ctaagcgaat
     7801 tactaaaaac cgctgaagtg ccgaaagggt ccttctatca ctactttcgc tctaaagaag
     7861 cgtttggcgt tgccatgctt gagcgtcatt acgccgcata tcaccagcga ctggctgagt
     7921 tgctgcaatc cggcgaaggt aactaccgcg accgcatact ggcttattac cagcaaacac
     7981 tgaaccagtt ttgccaacat ggaaccatca gtggttgcct gacagtaaaa ctctctgccg
     8041 aagtgtgcga tctgtcagaa gatatgcgca gcgcgatgga taaaggtgct cgcggcgtga
     8101 tcgccctgct ctcgcaggct ctggaaaatg gccgtgagaa ccattgttta accttttgtg
     8161 gcgaaccgct acaacaggca caagtgcttt acgcactatg gcttggcgcg aatctgcagg
     8221 ccaaaatttc gcgcagtttc gagccactgg aaaacgcgct ggcccatgta aaaaacatta
     8281 ttgcgacgcc tgccgtttag caggcatttt ttatcaccag acgaccggga gcctttatgt
     8341 catctgaaaa actgtattcc ccactgaaag tgggcgcgat cacggcggca aaccgtattt
     8401 ttatggcacc gctgacgcgt ctgcgcagta ttgaaccggg tgacattcct accccgttga
     8461 tggcggaata ctatcgccaa cgtgccagtg ccggtttgat tattagtgaa gccacgcaaa
     8521 tttctgccca ggcaaaagga tatgcaggtg cgcctggcat ccatagtccg gagcaaattg
     8581 ccgcatggaa aaaaatcacc gctggcgttc atgctgaaaa tggtcatatg gccttgcagc
     8641 tgtggcacac cggacgcatt tctcacgcca gcctgcaacc tggcggtcag gcaccggtag
     8701 cgccttcagc acttagcgcg ggaacacgta cttctctgcg cgatgaaaat ggtcaggcga
     8761 tccgcgttga aacatccatg ccgcgtgcgc ttgaactgga agagattccg ggtatcgtca
     8821 atgatttccg tcaggccatt gctaacgcgc gtgaagccgc ttttgatctg gtagagctcc
     8881 actctgctca cggttatttg ctgcatcagt tcctttcccc ttcttcaaac catcgtaccg
     8941 atcagtacgg cggcagcgtg gaaaatcgcg cacgtttggt actggaagtg gtcgatgccg
     9001 ggattgaaga atggggtgcc gatcgcattg gcattcgcgt ttcgccaatc ggtactttcc
     9061 agaacacaga taacggcccg aatgaagaag ccgatgcact gtatctgatt gaacaactgg
     9121 gtaaacgcgg cattgcttat ctgcatatgt cagaaccaga ttgggcgggg ggtgaaccgt
     9181 atactgatgc gttccgcgaa aaagtacgcg cccgtttcca cggtccgatt atcggcgcag
     9241 gtgcatacac agtagaaaaa gctgaaacgc tgatcggcaa agggttaatt gatgcggtgg
     9301 catttggtcg tgactggatt gcgaacccgg atctggtcgc ccgcttgcag ctcaaagctg
     9361 agcttaaccc acagcgtgcc gaaagtttct acggtggcgg cgcggaaggc tataccgatt
     9421 acccgacgtt gtaatccaac attgcgagcg gcgtaaagcc gccgctatac taaaacaaca
     9481 ttttgaatct gttagccatt ttgaggataa aaagatgcgt cttcttcata ccatgctgcg
     9541 cgttggcgat ttgcaacgct ccatcgattt ttataccaaa gtgctgggca tgaaactgct
     9601 gcgtaccagc gaaaacccgg aatacaaata ctcactggcg tttgttggct acggcccgga
     9661 aaccgaagaa gcggtgattg aactgaccta caactggggc gtggataaat acgaactcgg
     9721 cactgcttat ggtcacatcg cgcttagcgt agataacgcc gctgaagcgt gcgaaaaaat
     9781 ccgtcaaaac gggggtaacg tgacccgtga agcgggtccg gtaaaaggcg gtactacggt
     9841 tatcgcgttt gtggaagatc cggacggtta caaaattgag ttaatcgaag agaaagacgc
     9901 cggtcgcggt ctaggcaact aatctcctgc cgggcgtgaa ctcatcgcgc ccgcatcttt
     9961 actgcatcga caagtaatat ttgtcataat gcgcgctgca atttatccgt attaagagaa
    10021 tcagatgtcc gataacgctc aacttaccgg tctgtgcgac cgttttcgtg gtttttatcc
    10081 tgttgtgatc gatgttgaaa cagccggatt taacgccaaa accgatgcgc tgcttgagat
    10141 tgccgccatc accctgaaaa tggatgaaca aggctggctg atgccggaca ccacattaca
    10201 tttccacgtc gaaccatttg tcggcgcaaa tttgcaacca gaagccctcg ccttcaacgg
    10261 cattgacccg aacgatcccg atcgcggcgc ggtcagcgaa tacgaggcgc tgcacgaaat
    10321 ttttaaagtt gtacgtaaag gtattaaagc gagcggctgt aaccgcgcca ttatggtggc
    10381 gcacaacgcc aattttgatc acagctttat gatggccgcc gcagaacgcg cctcgctgaa
    10441 acgtaacccg ttccaccctt tcgccacttt tgacactgct gcactggccg ggctggcact
    10501 cggacaaacc gtattgtcaa aggcttgcca gaccgctggc atggacttcg acagcaccca
    10561 ggcgcactcc gcgctgtacg acaccgaacg cactgctgtg ctgttttgtg aaatcgtcaa
    10621 ccgctggaaa cgtctgggag gctggccgct acctgccgcc gaagaggtgt aatcgagtcg
    10681 atgcctgatg acatgcaatg attcaggcat ctatagtgag gctattccac gcatcaagca
    10741 tgatattcac ggggaatagc gttaatggca gataatccag acccttcatc gctcctgccg
    10801 gacgtgtttt caccggcgac ccgcgactgg tttcttcgcg cctttaaaca gccgaccgct
    10861 gtccagtcgc aaacctggca tgtggcggcg cgaggcgaac atgcgctggt gattgcaccg
    10921 actggctccg ggaaaacgct ggcagcattt ctctacgccc tcgatcggct cttccgcgaa
    10981 ggcggcgaag atacccgcga ggcgcataag cgtaaaacct cacgcatcct ctatatttca
    11041 ccgataaaag ccctgggcac cgacgttcag cgcaacttgc agatcccgtt gaagggtatt
    11101 gccgatgaac ggcggcggcg cggcgaaacg gtaattattg atgaagtcca cgcggtagcg
    11161 ggcagtaaac gtggtgcgca tctggcgtta agtctggagc ggctcgatgc gctgctccac
    11221 acctcagcac agcgaattgg cctttctgcc actgtgcgct cagccagcga tgtggcagca
    11281 tttcttggtg gcgatcgccc ggttacggta gtcaacccgc ccacaatgcg ccatccgcag
    11341 atacgaattg tcgtaccggt cgccaatatg gatgatgtct catcggtcgc cagcggcacc
    11401 ggcgaagaca gccatgccgg ccgggaaggc tccatctggc catatattga aacgggtatc
    11461 cttgatgaag tgttgcgcca tcgctcgacc attgtcttta ctaattcacg cgggctggcg
    11521 gaaaaactga cggcacgatt aaatgagctt tacgccgcac gcttacagcg ttccccgtct
    11581 atcgccgttg atgcggccca tttcgagtcg acctccggcg caacctctaa ccgtgtacaa
    11641 agtagcgacg tttttattgc ccgctcacac cacggctccg tctctaaaga acaacgagca
    11701 atcaccgaac aggcgctgaa atcgggtgaa ttacgctgcg tggtcgcaac ctccagtctt
    11761 gaactgggga ttgatatggg cgcggtggat ctggtgattc aggtggcaac gccgctttct
    11821 gttgccagtg ggttacaacg cattggtcgc gccggacatc aggttggcgg tgtatctaaa
    11881 gggctgtttt tcccccgtac ccggcgtgat ttagtcgatt ccgcagtcat tgtagagtgt
    11941 atgttcgcag gcaggctgga aaacctgaca ccaccgcata atcctctcga cgtccttgcg
    12001 cagcaaaccg ttgccgccgc ggcgatggat gcattacagg tagacgaatg gtactcccgc
    12061 gtacgccgta ccgcaccgtg gaaagatctg ccaagacgtg tttttgacgc cacgctggat
    12121 atgctttccg ggcgctatcc ctctggcgat ttttctgctt ttcgccccaa actggtctgg
    12181 aacagggaga ccgggatatt gaccgcccga cctggcgctc aattgttggc ggttaccagc
    12241 ggcggcacca ttccggatcg tggcatgtat agcgtgttat tacccgaagg tgaagaaaag
    12301 gccggttcgc ggcgggtggg tgaactggat gaggagatgg tatatgagtc gcgggtgaac
    12361 gacattatca ctctcggcgc tacctcatgg cggatccagc aaatcacccg cgatcaggtg
    12421 attgtgactc ctgctccggg tcgttctgcc cagctcccct tctggcgtgg tgaaggtaac
    12481 ggacgtccgg ctgaattagg cgagatgatc ggcgattttc ttcatttact ggcggatggc
    12541 gcgttctttt ccgggactat tcccccgtgg ctggcagaag aaaatacgaa cgccaatatt
    12601 caggggttga ttgacgagca acgcaacgcg acgggcatcg ttccggggag tcgccatctg
    12661 gtcctcgaac ggtgccgtga tgaaattggc gactggcgta ttattttgca ctctccctat
    12721 ggaagacggg tgcatgaacc ctgggcgctg gcgattgccg ggcgaataca tgccctatgg
    12781 ggcgctgacg cgtctgtggt cgccagtgat gacagcattg ttgcgcgcat tcctgacact
    12841 gatggtaaat tgcccgacgc cgcgattttt ttgttttaac cagaaaagtt gctgcaaatt
    12901 gtccgcgagg cggtaggcag ctcggcactt ttcgccgccc gttttcgcga atgcgccgcg
    12961 cgggcattat taatgccggg ccgcacgccg ggccatcgca ccccgctttg gcaacaacgg
    13021 ctgcgcgcca gtcagttgct ggaaatcgct cagggatatc cggattttcc ggtcattctc
    13081 gaaaccctac gcgaatgtct gcaagatgtt tatgatcttc ccgcactgga acgtttgatg
    13141 cgtcgcctga acggtggcga aattcaaata tccgatgtaa cgaccactac gccctcgcct
    13201 ttcgccacaa gtttattgtt cggctatgtc gcggaattta tgtaccagag cgacgccccg
    13261 ctggcagagc gccgggcatc cgtactgtcg ctggacagcg agttactgcg caatctactc
    13321 ggacaggtcg atccggggga attactcgac ccgcaggtca ttcgccaggt ggaagaagag
    13381 ttgcaacgac tggctcctgg tagaagagcg aaaggtgaag aaggattgtt cgacctgctg
    13441 cgcgaactgg ggccaatgac cgttgaagac ctggcgcaac ggcatacagg cagcagtgaa
    13501 gaggttgcgt cgtatctgga aaatcttctt gcagtaaaac gaatcttccc agcgatgatt
    13561 agcggacagg agcgtcttgc ctgtatggat gatgccgcca ggctgcgtga tgccctcggc
    13621 gtacggctac cagagtcatt gccagagatt tatttacata gagtcagtta cccgcttcgc
    13681 gacctctttc tgcgctatct ccgggctcat gctctggtca cggctgaaca actggctcat
    13741 gagtttagtc tcggtattgc cattgtcgaa gagcagcttc agcaactgcg tgaacagggt
    13801 ctggtgatga atctgcaaca agacatctgg gtgagcgatg aagtatttcg tcgtctgcgt
    13861 ttgcgctcgc tgcaagccgc cagagaagcg acgcgtcccg ttgcagccac gacctatgcg
    13921 cgattgctgc tggaacgtca gggcgtatta cccgccaccg atggtagccc ggcgctcttt
    13981 gcctcaacat ctccaggcgt ttatgagggc gtagatggag tgatgcgggt gatcgaacag
    14041 cttgccggag tcggtttacc cgcctcactc tgggaaagcc agatcctgcc tgcccgcgta
    14101 cgcgactatt cgccagaaat gctcgatgaa ttactggcaa ccggagcggt tatctggtcg
    14161 ggacaaaaaa aagctgggtg aagatgacgg cctggtggca ctgcatctac agaaatatgc
    14221 cgcagaatcg ttcactcccg ccgaagcgga tcaggcgaat cgttcggcgc tgcagcaagc
    14281 gataatcgct gttctggctg acggaggagc ctggtttgca caacaaatca gccaacggat
    14341 acgcgataaa atcgtcgaat cggttgattc ctcggcactg caagaggcgt tatgggcgct
    14401 ggtctggcaa ggcgtcatta ccagcgatat ttgggcaccg ttacgcgccc tcacccgcag
    14461 cagttccaac gcacgcacct caactcgccg cagtcaccgg gctcgtcgtg ggcgtcctgt
    14521 ctatgcacaa cccgtctcgc cgcgggtatc ttacaacaca ccaaatctgg ctggacgctg
    14581 gtcgttattg caggtggagc cactaaacga taccgaaagg atgctggcgc tggcggaaaa
    14641 tatgctcgac cgctacggca tcatcagtcg tcaggcggtg atagccgaaa atatccctgg
    14701 cgggtttcca tcgatgcaaa cactttgtcg aagtatggaa gactccgggc gaattatgcg
    14761 aggtcgtttt gtagaaggtc tgggtggcgc gcaattcgct gaacgtctga ctattgaccg
    14821 attgcgcgat ctggcgacac aagccacgca aacacgccac tatacaccag tggcgctctc
    14881 cgccaacgat ccggctaatg tgtggggaaa tcttctgccc tgggctgcac atccggcaac
    14941 gctggtacca acgcgtcggg cgggtgcgct ggtggtcgtt tctggcggca aattgttact
    15001 ctatctggcg caaggcggca aaaaaatgct ggtctggcag gaaaaggagg aattactcgc
    15061 cccagagatt ttccaggcgc tgaccaccgc actgcgtcgc gaaccaaggc tgcgctttac
    15121 gctaacagaa gtgaatgatc ttccggtccg gcaaacgccg atgtttacgc tgctgcgtga
    15181 ggcgggattt tcaagttcgc cacaagggct ggattgggga tagttgacat cctccacgcc
    15241 ctgaaagacg tggttccctg ctacgttcaa gctgtcgcct gaatcatttc ggtgggttcc
    15301 tgcttcaacg agcggcctga ctgcaccatc tctccacagg caagaacggc gtgccccgcc
    15361 gctaaaatgt tacgagcgcc atttacatcg gcgttcgctg tatatccaca tacctggcat
    15421 ctgaatttac tttgtgacag gcggttttct ttcgctgtat gaccacagca cgcgcaacgc
    15481 tggcttgtgt acgctggcgg aacagcaagc acctgaccgc cactccagag ctgcttgtac
    15541 tcaagctggc ggcgcatttc ataccagccc tgatccagta tcgaacggtt taaacctgat
    15601 tttgcccgga cattgcgccc cggctggctg accgtacccg ctgctgactt tgacatgttg
    15661 ctgaccttca aatcctcaat gactatcatt gcgtggtttt tgctgacggt cgttgtgact
    15721 ttgtgaaggt agtccctgcg gatatttgcg atacaggaat gcagtcgctg tattttgcgt
    15781 ttctgcttct gccagttgtt gctgaatttg accttccggc ttaactgtcg ctgaagtctc
    15841 gccagcgtct tctggttttt ttgaaaactg tttacaggct caaagactgt gccatctgac
    15901 agcgtggcga gtttagccac gccagcatcc agcccgatca ttgatgctga agggtgaacc
    15961 ggagttgata cttcactttc tgtctgaata ctgatgtacc acgtaccgca ggactggctg
    16021 acagtgacat ttttcacaac tcccgtgacc tgacggctat tccggtagcg catccagcca
    16081 agtttcggca gaaaaatacg gctgttttcc tgatcgagct taacgtcctg cgggtagcgg
    16141 aatgcagcat tctgtccccg ctttttgaat cggggaaaag cagcccgctt ctggaagaag
    16201 tttttgtaag cccgctcaag gtctttcagt gactgttgca atggctgtga gggcgaatct
    16261 ttaagccatt gcgtttcagt ggcgtttttc cactcaacca gccaggaagc cattttcccg
    16321 taagggatgt atttattacc ggcctcatga ttctcattct gaagcgccag agcacgattg
    16381 aaaacgaaac gacatgcgcc tgcgaagcgc ctcatttcac gctcctgttg accaccgggt
    16441 cttaactgga atttaaatgc ctgtaatcgt ttcacaggtc aaatgatact ttggtctgtg
    16501 aaaaaagaaa ccgatatccg gcgtggaagg cattgtgttt tcctgatgca tgttcacctg
    16561 gtctttgtca ccaaataccg acgccagatt tttgaccatg acacgacaga aaaactacgc
    16621 acttactttt caaatgtatg tgctgatttt gcagctgaac tggttgaaat ggatggcgaa
    16681 ccagatcacg tccatttgtt aattaactat cctcccaaac tggcgatatc cagtctggta
    16741 aacagcctca aaggcatatc gaataggtta ctgcgacgag atcgaccaga tattgcagtc
    16801 aggtattact acaaaggcgt tttgtggagt cctggctatt ttgccagtag ctgcgaaggt
    16861 gcgccaatat ccgtcatccg ccagtacatt gaacaacagc aaacacctgg tcaggtggaa
    16921 aaccgcgcct tatatccccg ccctgaagga cgaggtttta cggcgcaccg gataaaggac
    16981 tgacggatgc ccgttcgcat ccgtcagtat tgcaggacgg attattccgc gtccggctct
    17041 tcagacttgt atttagcggc agtttctttg atcagctgct gcagttcgcc acgctgatac
    17101 atttcgatca cgatatcaca accgccgacc agctcgccgt caacccacag ttgcgggaag
    17161 gtcggccagt tagcatattt cggcagttcc gcacgaatgt ccggattctg cagaatatca
    17221 acataggcaa aacgttcgcc acatgcggca agcgcctgga ctgcctgggc agagaaaccg
    17281 cagctcggca gtttcggtga acctttcatg tacagcagga tcgggttttc agcaatctgg
    17341 cgttggattt tttcgatagt ggtgctcatt gtcttgcttc ctcaaacttc tttacggcag
    17401 taatctgaca ttgtagcggg tcagtgcggc atcggaaaat aacattttca tcacgctttt
    17461 gctattttat ccctttgctc tatttgttgc atttcaaata ttcgtttctt ctctgatgcg
    17521 cggcaaactg gcgtatttat aaccattttt agctttcact gctgcatttt tttgcgctcg
    17581 ccaacgaaac gtatttttta acaataaaag ctattaactt tctcttcttc tatgcattag
    17641 aatcatcaag ttttgtaaat cagacgcagg catgatagac ctgccttaac agagggacgc
    17701 tcagtggcgc ggataaaccg tatttcgatc acgctctgtg ctttactttt taccaccctg
    17761 cctttaacgc ctatggccca tgcttcaaag caagccaggg agagttctgc taccactcat
    17821 atcaccaaaa aagcagataa aaagaaaagc acggcaacca ccaaaaagac ccagaaagcc
    17881 agccagaaaa cagcgaaaaa agccgccagt aaaagtatga ccaaaagcaa aaccgcttct
    17941 tctgttaaaa aatcttccat taccgcttct aaaaacgcca aaactcgcag caaacacgcc
    18001 gtcaataaaa cggcctcagc cagctttacc gaaaagtgta ccaaacgtaa gggctataaa
    18061 tcgcattgtg tgaaagtcaa aaatgccgcg tcaggaaccc ttgccgacgc gcacaaagcg
    18121 aaggtgcaaa aagccacgaa agtggcaatg aataaactga tgcagcaaat tggtaagcca
    18181 tatcgttggg gcggcagctc accgcgtacc ggttttgatt gcagcggcct ggtttattac
    18241 gcttataaag atttggtgaa aattcgtatt ccgcgtacgg cgaatgaaat gtatcacctg
    18301 cgtgatgcag gacctatcga acgtagtgag ctgaaaaacg gcgacctggt ctttttccgt
    18361 actcagggac gcggtacagc cgatcatgtc ggcgtgtatg tcggcaacgg caaatttatt
    18421 cagtcaccgc gcacaggtca ggaaattcaa atcacttctc tcagtgaaga ctactggcag
    18481 cgccactatg ttggcgctcg tcgggtaatg accccaaaaa cacttcgcta aaactttacc
    18541 ctgttgttac ggcaacaggg taagttcatc ttttgtctca ccttttaatt tgctacccta
    18601 tccatacgca caataaggct attgtacgta tgcaaattaa taataaagga gagtagcaat
    18661 gtcattcgaa ttacctgcac taccatatgc taaagatgct ctggcaccgc acatttctgc
    18721 ggaaactatc gagtatcact acggcaagca tcatcagact tatgtcacta acctgaacaa
    18781 cctgattaaa ggtaccgcgt ttgaaggtaa atcactggaa gagattattc gcagctctga
    18841 aggtggcgta ttcaacaacg cagctcaggt ctggaaccat actttctact ggaactgcct
    18901 ggcaccgaac gccggtggcg aaccgactgg aaaagtcgct gaagctatcg ccgcatcttt
    18961 tggcagcttt gccgatttca aagcgcagtt tactgatgca gcgatcaaaa actttggttc
    19021 tggctggacc tggctggtga aaaacagcga tggcaaactg gctatcgttt caacctctaa
    19081 cgcgggtact ccgctgacca ccgatgcgac tccgctgctg accgttgatg tctgggaaca
    19141 cgcttattac atcgactatc gcaatgcacg tcctggctat ctggagcact tctgggcgct
    19201 ggtgaactgg gaattcgtag cgaaaaatct cgctgcataa taactgatgg caaatgcagc
    19261 attgcctgat gcgcttcgct taccatgcca acgcggatga ggcgttaaca gcgcattcgg
    19321 caagataaat cgcacgttgt cagcaactgt aacgcagaag gttatccttc tgcgtttttg
    19381 tttaattagc tgttagcaac gcaaactgtt tcaggttgtt ttctggctga cataaacacc
    19441 agcaataatg ccagtcccgc gacaatcgcc cccgtcaccg gcacaaagct gtatcccagc
    19501 ccagcggaaa ttaccgcacc accagcagct gctcccagcg catttccaag attaaaggca
    19561 ccaatattga ctgatgaaga cagacctggc gcttcactgg cgacatgcat cacgcgcatc
    19621 tgtaacggag gtacaaccgc aaaggttgct gcgccccaca ccaccatgct aatagctgcg
    19681 ccgaactcat tgcgggccag gaacgggatt gccagcataa tcaccatcag cagcaacaaa
    19741 aagcctttca acgtgccgtt aactgaacga tctgccagtt tgccgccgag atagttaccg
    19801 atagagaatc cgacaccaat cagcaccagc attgccgtga cgaacaccgg tgttgcgtgg
    19861 gtaatacttt gcagtaccgg agagatatag gtgtagaggg taaacattgc accagctccc
    19921 agtaccgtcg tcagcaatgc agacagcacc tgcggacgca ttaataccgc cagctctttt
    19981 ttcacttcag gtcgtgcccc tgtaccacct ttaggtaatg agaagaacag acttaccatt
    20041 gaaatcactc ccagccccgc cgttgccaga aatgacatcc gccagccgat ggtttcaccc
    20101 aaccaggtcg ccgccggcac gccaccgata tttgccaggg ttaaccccat aaacatagtg
    20161 gcaactgcgc tggcctgttt atgttttggc accacgcttg cggccacgac tgaacccaaa
    20221 ccaaaaaatg ctccgtgatt caggctggtc aaaatacgtg aaagcatcag ggtcatataa
    20281 tccggcgcga tggcggaaag aacgttgcct agcgtgaaaa ttgccatcag gaaaatcaac
    20341 gcactgcggc gggcacgatg agaaagtaga agcgtcatca gcggcgcgcc caccattacg
    20401 ccaactgcat aggcactgat taacattccg gcagcgggaa tcgagacatc cacaccgcgc
    20461 gcaatgacgg gcaacaagcc cattggcgag aactccgttg tcccgatacc aaacgcgcca
    20521 atcgccagcg ccagcaacgg atagttaatt ttcatgcctt atctccacct cttcgcgtca
    20581 ttacgcgata ttcattaaag tggcgaaagc atgacagcaa tcacaaaaaa atgaaaataa
    20641 caaaaagaga aaacactttt gccattttgc taacaaacag gaaggagatg cgagggagaa
    20701 cgcgctccct cgagaggaaa tcagtgcagc gcggcagtca aacccacggc tacgatcaaa
    20761 ccgaggacga taaccgttgt taccagtgaa aatttaaggt cggtgctcat caagttttct
    20821 ccttttttat taccacacaa aaagtgatat tacgcatttt tacacactgt gatgaaaaaa
    20881 tctcccgtca tttataatga taagtgtttt taccacttcc ccttttcgtc aagatcggcc
    20941 aaaattccac gcttacacta tttgcgtact ggccattgac cccttcctga cgctccgtgt
    21001 cgtttttccg gcgtaccgca acacttttgt tgtgcgtaag gtgtgtaaag gcaaacgttt
    21061 accttgcgat tttgcaggag ctgaagttag ggtctggagt gaaatggaat ggcaacaata
    21121 aaagatgtag cgaaacgagc aaacgtttcc actacaactg tgtcacacgt gatcaacaaa
    21181 acacgtttcg tcgctgaaga aacgcgcaac gccgtgtggg cagcgattaa agaattacac
    21241 tactccccta gcgcggtggc gcgtagcctg aaggttaacc acaccaagtc tatcggtttg
    21301 ctggcgacca gcagcgaagc ggcctatttt gccgagatca ttgaagcagt tgaaaaaaat
    21361 tgcttccaga aaggttacac cctgattctg ggtaatgcgt ggaacaatct tgagaaacag
    21421 cgggcttatc tgtcgatgat ggcgcaaaaa cgcgtcgatg gtctgctggt gatgtgttct
    21481 gagtacccag agccgttgct ggcgatgctg gaagagtatc gccatatccc aatggtggtg
    21541 atggactggg gtgaagcaaa agctgacttc accgatgcgg tcattgataa cgcgttcgaa
    21601 ggcggctaca tggccgggcg ttatctgatt gaacgcggtc accgcgaaat cggcgtcatc
    21661 cccggcccgc tggaacgtaa caccggcgca ggccgccttg ccggttttat gaaggcgatg
    21721 gaagaagcga tgatcaaggt gccggaaagc tggattgtgc agggtgactt tgaacctgaa
    21781 tccggttatc gcgccatgca gcaaatcctg tcgcagccgc atcgccctac tgccgtcttc
    21841 tgtggtggcg atatcatggc aatgggcgca ctttgtgctg ctgatgaaat gggcctgcgc
    21901 gtcccgcagg atgtttcgct gatcggttat gataacgtgc gcaacgcgcg ctattttacg
    21961 ccggcgctga ccacgatcca tcagccaaaa gattcgctgg gtgaaacagc gttcaacatg
    22021 ctgttggatc gtatcgtcaa caaacgtgaa gaaccgcagt ccattgaagt gcatccgcgt
    22081 ttgattgaac gccgctccgt ggctgacggc ccgttccgcg actatcgtcg ttaatcaccc
    22141 gttgcgggag tctcttccgg ctcccgcagc cactccttat tcagcgtctc actatcgctg
    22201 agatactcaa gcagccaggt taacgcaggc gacatatcat tttgctgcca tgtcagacaa
    22261 catgccgaat ccgggaaggg gttttccagt tcaagtgcta cccacttccc ctcattaagc
    22321 cacggtttgg cgaaatgtgt tggcaccatc cctatgcata atcctgccga gatacaggtt
    22381 gccgatgatt cccagtcagg tacgacaagt cttttttggt tatccagcaa ccaggtaata
    22441 cgtttaggta gcgttcgcga ggtgtcttcg cgcaccaacg acggccagtt gcgcaacgta
    22501 tcatcgctga acgggccatc catcaacgcc agcgggtggt gactggcaac aacgcaactc
    22561 cagcttagca tccccatatc ccggaaggca taacgaccgc ctaccggaat cgcgcgtgtt
    22621 gcgccaattg ccagttccac gcgcccgtcg gaaagcgcat cccagacacc gttgaacact
    22681 tcctgaaaga caagaagttc gacatcatca aaatggcgat aaaaatcaac gatcatctgc
    22741 cgtgtacgtt ctggcctgac aatattatcc actgcgatag ctaactgacc gcgccagccg
    22801 ttcgctatct gctgacattg ctggcgggtg atctgcattt ttttgacaac agagcgccct
    22861 tctttgagaa accacgctcc agcagcggtc agctccacat cacggtgccg tcgttcaaag
    22921 agcggtaccg ccagccactc ttccagctga cgcacggtat agctgaccgc agaaggaaca
    22981 cgatgcagct cctgtgccgc agcgctaaaa ctaccattac gcgctaccgc atcaacaact
    23041 tcgagtgaat attctgacca catagtctgc ctgcaaaatt tttgaaacca gtcatcaaat
    23101 attaccgttt cacaacacta atttcactcc ctacactttg cggcggtgtt taattgagag
    23161 atttagagaa tatacatgca acctgggaaa agatttttag tctggctggc gggtttgagc
    23221 gtactcggtt ttctggcaac cgatatgtat ctgcctgctt tcgccgccat acaggccgac
    23281 ctgcaaacgc ctgcgtctgc tgtcagtgcc agccttagtc tgttccttgc cggttttgcc
    23341 gcagcccagc ttctgtgggg gccactctcc gaccgttatg gtcgtaaacc ggtgttatta
    23401 atcggcctga caatttttgc gttaggtagt ctggggatgc tgtgggtaga aaacgccgcc
    23461 acgctgctgg tattgcgttt tgtacaggct gtgggggtct gcgccgcggc ggttatctgg
    23521 caagcattag tgacagatta ttatccttca cagaaagtca accgtatttt tgcgaccatc
    23581 atgccgctgg tgggtctgtc tccggcactg gctcccctgt taggaagctg gctgctggtc
    23641 catttttcct ggcaggcgat tttcgccacc ctgtttgcca ttaccgtggt gctgattctg
    23701 cctattttct ggctcaaacc cacgacgaag gcccgtaaca atagtcagga tggtctgacc
    23761 tttaccgacc tgctacgttc taaaacctat cgcggcaacg tgctgatata cgcagcctgt
    23821 tcagccagtt tttttgcatg gctgaccggt tcaccgttca tccttagtga aatgggctac
    23881 agcccggcag ttattggttt aagttatgtc ccgcaaacta tcgcgtttct gattggtggt
    23941 tatggctgtc gcgccgcact gcagaaatgg caaggcaagc agttattacc gtggttgctg
    24001 gtgctgtttg ctgtcagcgt cattgcgacc tgggctgcgg gcttcattag ccatgtgtcg
    24061 ctggtcgaaa tcctgatccc attctgtgtg atggcgattg ccaatggcgc gatctaccct
    24121 attgttgtcg cccaggcgct gcgtcccttc ccacatgcaa ccggtcgcgc cgcagcgttg
    24181 cagaacactc tgcaactggg tctgtgcttc ctcgcaagtc tggtagtttc ctggcttatt
    24241 agtatcagca cgccattgct caccaccacc agcgtgatgt tatcaacagt agtgctggtc
    24301 gcgctgggtt acatgatgca acgttgtgaa gaagttgact gcccgaatca tggcaatgcc
    24361 gaagtcgctc atagcgaatc acactgatct atatcgatat acttatactt aggctgctaa
    24421 caaaattttg ttgtatgatt gaaattagcg gcctatacta atttcgagtt gttaaagcta
    24481 cgataaatat tatgttttta cggggacagg atcgttcccg actcactatg gatggtcatt
    24541 tcggcaaggg ttcctccttt ccctctgttc tacgtcggat tatagactcg cggttttttc
    24601 tgcgagattt ctcacaaagc ccaaaaagcg tctacgctgt tttaaggttc tgatcaccga
    24661 ccagtgatgg agaaactatg agttcatcgt gtatagaaga agtcagtgta ccggatgaca
    24721 actggtaccg tatcgccaac gaattactta gccgtgccgg tatagccatt aacggttctg
    24781 ccccggcgga tattcgtgtg aaaaaccccg atttttttaa acgcgttctg caggaaggct
    24841 ctttggggtt aggcgaaagt tatatggatg gctggtggga atgtgaccga ctggatatgt
    24901 tttttagcaa agtcttacgc gcaggtctcg agaaccaact cccccatcat ttcaaagaca
    24961 cgctgcgtat tgccggcgct cgtctcttca atctgcagag taaaaaacgt gcctggatag
    25021 tcggcaagga gcattacgat ttgggtaatg acttgttcag ccgcatgctt gatcccttca
    25081 tgcaatattc ctgcgcttac tggaaagatg ccgataatct ggaatctgcc cagcaggcga
    25141 agctcaaaat gatttgtgaa aaattgcagt taaaaccagg gatgcgcgta ctggatattg
    25201 gctgcggctg gggcggacta gcgcactaca tggcatctaa ctatgacgta agcgtggtcg
    25261 gcgtcaccat ttctgccgaa cagcaaaaaa tggctcagga acgctgtaaa ggcctggatg
    25321 tcaccatttt gctgcaagat tatcgtgacc tgaacgacca gtttgatcgt attgtttctg
    25381 tggggatgtt cgagcacgtc ggaccgaaaa attacgatac ctattttgcg gtggtggatc
    25441 gtaatttgaa accggaaggt atattcctgc tccatactat cggttcgaaa aaaaccgatc
    25501 tgaatgttga tccctggatt aataaatata tttttccgaa cggttgcctg ccctctgtac
    25561 gccagattgc tcaatccagc gaaccccact ttgtgatgga agactggcat aacttcggtg
    25621 ctgattacga tactacgttg atggcgtggt atgaacgatt cctcgccgca tggccagaaa
    25681 ttgcggataa ctacagtgaa cgctttaaac ggatgtttac ctattatctg aatgcctgcg
    25741 caggtgcttt ccgcgcccgt gatattcagc tctggcaggt ggtgttctca cgcggtgttg
    25801 aaaacggcct tcgagtcgct cgctaaaggc tattctatcg ccccctcttc gggggcgagt
    25861 tcagatcagg cttctgtacc tggttgattc atggcatttt ctcgtgccgc cagcacacgt
    25921 tctaccgtat ctaccactgc ctgagtttgt ggatcgattt caatgttgac gcgtgcgcca
    25981 agttttttct tcccaagagt cgtgcgttcc agtgtttccg gaattaaatg gacgcaaaaa
    26041 cgcgttggcg tgacttcgcc gacggtcagg ctaataccgt cgatgccaat aaatcctttg
    26101 tacaggatat atttcatcaa ctgactatcc tggactttaa accagatctg gcgattattt
    26161 tccgaggtta atattttcgc cacttcagcg gtggtcataa tatgacctga cattaagtgt
    26221 ccgccaattt catcactgaa tttcgccgca cgctcaacgt ttacccaatc ccccactttt
    26281 aaatcgccaa gattggtaat gcgtaacgtt tctttcatca ggtcaaaact gacatggttg
    26341 ccgttaattt ccgtcacggt caggcagcaa ccgttatgcg ccacggaagc accggtttcc
    26401 aggccgtcca gcatgtggtc gggtaactcc accacatgcg tacgaaaatt tggtttctcg
    26461 tcaatcgaca ccagttttgc ggtgccctgt acaatccccg taaacatact tacaactcct
    26521 gaaatcagtt aagacattct gttcagcaca atagcaggtg gaaaacgccc ttaccagtga
    26581 aggggtaaga atggctattt tttcactgga gaattaataa atcctcgcta caatagactg
    26641 aatttcccct gcttcttctt tttgctgccc attcaggcgg ctttttagtc tctcatataa
    26701 ctacaaataa aaggtgttca cgtgcagaag tatatcagtg aagcgcgtct gttattagca
    26761 ttagcaatcc cggtgattct cgcgcaaatc gcccaaactg cgatgggttt tgtcgatacc
    26821 gtgatggcgg gcggctatag tgccaccgac atggcggcgg tcgctatcgg tacttctatc
    26881 tggcttccgg cgatcctctt tggtcacgga ctgctgctgg cattaacgcc ggttatcgcg
    26941 caattaaatg gttccggtcg acgtgagcgc attgcgcatc aggtgcgaca aggtttctgg
    27001 ctggcaggtt ttgtttccgt tctcattatg ctggtgctgt ggaatgcagg ttacattatc
    27061 cgctacatgg aaaacatcga tccggctctg gcggacaaag ccgtgggtta tctgcgtgcg
    27121 ttgttgtggg gcgcgccggg atatctgttc ttccaggttg cccgtaacca atgtgaaggt
    27181 ctggcaaaaa ccaagccggg tatggtaatg ggctttatcg gcctgctggt gaacatcccg
    27241 gtgaactata tctttattta tggtcatttc ggtatgcctg agctcggtgg cgttggttgt
    27301 ggcgtggcta ctgcggcggt gtattgggtc atgttccttg ccatggtttc ttacattaaa
    27361 cgcgcccgct ccatgcgcga tattcgtaac gaaaaaggca ccgctaagcc cgagcctgcg
    27421 gttatgaaac gactgattca actcggtttg ccgattgcgc tggcactgtt ctttgaagtg
    27481 acactgtttg ccgtcgtggc tctgttagtg tctccgctcg gtattgttga tgtcgcagga
    27541 caccagattg ccctgaactt tagttcactg atgtttgtgc ttccaatgtc gctggcggca
    27601 gcggtaacta tccgcgtagg ttatcgtctg ggtcagggct caacgctgga tgcgcaaacc
    27661 gctgcgcgga ccgggcttat ggtgggtgtc tgtatggcaa ccctgacggc cattttcacg
    27721 gtttcactgc gggagcaaat cgccctgttg tacaacgaca atcccgaggt tgtaacgctg
    27781 gctgcgcatt tgatgttgct ggcggcggta tatcagattt ctgactcaat ccaggtgatt
    27841 ggcagtggga ttttgcgtgg ttataaagat acgcgttcca ttttctatat cacctttaca
    27901 gcttactggg tgctgggctt gccaagcggc tatattctgg cactgaccga tctggtcgtt
    27961 gaacctatgg ggccagcagg cttctggata ggctttatta tcggcctgac atcggcagcc
    28021 attatgatga tgctgcgtat gcggttcctg caacgtatgc cgtcagccat cattctgcaa
    28081 cgagcgtccc gctaataaag acaaggcgca accttcacgg gttgcgcctg tgtttttacg
    28141 caggctggag cgttgcgcca atcccgtctt cgtctggctg taatttcaga gcgttacaca
    28201 gagtttgccg actggcatct gttatctcaa cgcgtatatc cccgttaact tcccctttca
    28261 cattgatgaa gtcaggtgaa atttctttcg cactggtttg caggcaaatc gctccctctc
    28321 cgcgcaggcg tttcactttc agttgcacat gcgttgccgc accagtgatc agccacagtt
    28381 ttccgccatt cagattgatg ttaccaacaa tgaaagtgac gctgtcacct tttaccgtac
    28441 tgccgtcgag aatcaacaca ccgccctgct gtacagtaat cgttgattgt tgcagctctg
    28501 cgccataacc aataatcatt gaagcgccgt ccagtaatgc aacactcccg gtcagttcaa
    28561 ccggtgcgcg atcttcctct acgacaaaat tttcgtccgc gctggtgggt gacaacagga
    28621 tcactgttcc accttgtatt accgcatttt ccagcgtacc ggcatgcact tcagcccgcc
    28681 cccccacatt caccgacagg ttttgcgtct taccggaact gtagagctga aggccatccg
    28741 tccccagacg ataaatggat ccattctcaa caatagtgtc agagtcttca ccaccgagat
    28801 tattcatggt gccaccagca agcaacgtat cactggcttt cccggccagc ataaaaacgc
    28861 cgccctgatt aatctcacaa ttgctggctt ctccacttgg atacaccaac aattcaccac
    28921 cttcatctac cacgaccgct gaggttgtcc cattaaccaa caggccgccc tcttgatcga
    28981 gaatgatttt ttccgcgcga tgtccttcca gtacacgcag gttaccgcca ttttccagca
    29041 acaaaccgca ggcataaccc tgatcaacgc tgaattcacc ttcgggatgg cggccattaa
    29101 ccgtagcgag cgtagagaga ctaattgcgc ctccctcgga ctgaacaata tctgtggcaa
    29161 tggcaccgtc gctgaccgat agcgtgccat tgttaccgta ctgtgtgcat ttggcgatcc
    29221 caccggttgc cactttcaac cagccagccc ccgtgaccac ggtattttcg gctcttccgc
    29281 cagcttcaac aatcaactcg ccttcagtaa gttgcgtcgc gccaaggacc tcgccggtct
    29341 taacaatttg cacattcccg tcgctcatca agtttttcgc atcagatccc atgatttatt
    29401 cctttgctgc atctgtgtgc ctttattgct acctaagtgt aaaggctacg gaggatttat
    29461 ccacgacaga tttgagatgg tggcaaacaa ctctgtttaa actctgatac acgaattatt
    29521 gggttgtatc agatgtaaat gcgatcctga ataaaaatcg cccttgcaaa tcaacaaaat
    29581 atagccaatt tgcttaaaag ctctgcaaac ggtcgaaata gcgcagaaaa ttacgttttg
    29641 cctcttgcca cctttccact ctgccgctaa tattcgtccc cgttgtcacc tacaacgttg
    29701 cgttcatagc tcagttggtt agagcaccac cttgacatgg tgggggtcgt tggttcgagt
    29761 ccaattgaac gcaccatcct gcgtccgtag ctcagttggt tagagcacca ccttgacatg
    29821 gtgggggtcg gtggttcgag tccactcgga cgcaccagat tttcttaatc tggtcttttc
    29881 ctttttccct ctgtttcttc tctgtatcca atacgttaaa agatttacac tgtcttcgta
    29941 tgcgttatca gaaggagaat cgctatggca actttgttac aacttcattt tgcttttaat
    30001 ggcccatttg gcgacgcaat ggctgagcag cttaaaccac ttgctgagtc gattaatcag
    30061 gaacctggtt ttctgtggaa ggtatggaca gaaagtgaaa agaaccacga agccggtggg
    30121 atctaccttt tcactgatga aaaaagcgcc cttgcctatc tggaaaaaca tactgcccga
    30181 ctgaaaaatc tcggcgttga ggaagttgtc gccaaagttt tcgatgtcaa tgagccactt
    30241 agtcaaatca atcaggcaaa actcgcctga cagaatttaa tcaagggcgg ttagcgccct
    30301 tttcatccct gtctgaaatt tctcaaattc taaaaatctc aaccaaactt atctgataac
    30361 actaaattcg aaagaatgcg tacaggtaag taacaatgaa aaaaattgct attgtgggtg
    30421 ccgggcctac ggggatctac accttattct cgcttctaca gcaacaaact ccactttcta
    30481 tttctatctt cgagcaggct gacgaggccg gtgtcgggat gccatacagt gatgaggaaa
    30541 actcaaaaat gatgctggca aatattgcca gtattgaaat accgccgatt aattgtacgt
    30601 atctcgaatg gctacaaaag caagaagcca gccatctcca gcgttatggc gttaaaaaag
    30661 aaaccttgca cgatcgtcag tttttaccgc gaattctgct gggcgaatat ttccgcgatc
    30721 aatttttacg attagtagac caggcacgac agcaaaaatt tgcagtggct gtttatgaat
    30781 catgccaggt taccgatctg caaattacaa atgctggcgt catgctcgct acaaatcagg
    30841 atttacccag cgagacgttt gatttagcgg tgattgccac gggtcacgtc tggcctgatg
    30901 aagaagaagc aaccagaacg tattttccca gcccgtggtc aggcctgatg gaagcaaagg
    30961 tcgatgcgtg taacgtgggt attatgggaa catccttgag cggactggat gcggcaatgg
    31021 cagtggctat tcagcatggt tcgttcattg aagatgataa acaacacgtc gtttttcacc
    31081 gcgataacgc aagtgaaaag ctaaatatta cgttgatgtc gcgcacgggt attttacccg
    31141 aagccgattt ctattgccct attccctacg agcccttaca catcgtcacc gatcaggcat
    31201 taaatgctga gattcaaaaa ggtgaagagg gccttttgga tcgggtattt agattgatag
    31261 tagaggaaat caagtttgct gatccagact ggagccaacg catagcctta gagagcctga
    31321 atgtcgattc ctttgctcaa gcctggtttg ccgagcgcaa acaacgcgac ccatttgact
    31381 gggcagaaaa aaatctccag gaagtcgaac gcaataaacg agaaaaacat actgttccct
    31441 ggcgttatgt cattctgcgc ctgcatgaag ccgtacagga aattgttcct catctgaatg
    31501 aacacgacca taaacggttc agtaaaggcc ttgccagggt tttcatcgat aattatgcgg
    31561 caatcccttc agagtctatt cgtcgactac tggccttacg tgaagcgggg atctgaaatc
    31621 cctgatgttg gcgaagatta tacgttacag caacccgaag atattcgtgg acgcgtagcg
    31681 ttcggcgcgt tgccctggtt gatgcacgac cagcctttcg ttcagggact tacggcatgt
    31741 gcagaaattg gtgaggcgat ggctcgggcg gtcgtaaagc ctgcatcccg tgctcgtcgg
    31801 cgtctttcgt ttgattaaag gtaaagctat cagcactcaa caaactgatg ctgttcatta
    31861 agacgatagc ggactccggt tcgaatattg ttttcacctt caatggcgac ggcgaagcga
    31921 actctttcgc cgtcatgata tgccagtgcg gcgcttcccc ctagcccgag aataattgag
    31981 tcgaccacgc ccgtgctcac taccgttgaa ttttcaccgc tggcaatgat gtgtacattg
    32041 tcgccgctgt tggcaattct ggcgtttgcg ccaaagctgg cgatttgcac caggtcacca
    32101 ttactggcga catgacaccg ttcacccaat gtacaaacgc gtactcgcat tccggtattc
    32161 gcaatccgac tactatctcc agcgctgctt atccgcgaag aattaccggt tacagcaacc
    32221 cgtgcgcgtt caccggagct gccaatgtga ctgttaaagc cgacacttcc aatccgcacg
    32281 ctataaccca tactggcaat ttgcgcggca tacccagcgg ttgcgattcg ggcgttatcc
    32341 tcacaacagc cgatgcgagc atgatcgtcg ctgcgggcaa actgactgca aaatggtatc
    32401 tctccggggt ctgtggccag tttttgcata gaggaaactt cctgatgcgc gaagttttca
    32461 tccgccagcc attttttcca cgcatactca accagactct ccgcccagtc cgtatatcct
    32521 tcctcaatca gtgcatgacg aatatcggca taactaccgc cgcgtgggaa atggcgcagg
    32581 aaccagcgat acatcaccgc tccgatacgc cattctcgca ggtctgctcg tgtaatgatc
    32641 attcgttagt gccggtgata gccatcgacc atgcttttaa aggtttcgtg tggcgtacgc
    32701 cccgtggtgc aaagataaag atgaccgaag ataaaaaaga ggcttataaa tgccagagca
    32761 aaatgcgcct gcaataacca gtatcttacg ccaggaaaca catctcccac ggcttgcgga
    32821 tagagacaca acagccccgt caatagtaac aacggcagca atccatacat gacaccaaca
    32881 taggcgacct gctgtaaggg attaaattta gactgtgttg ttgccgggaa aggatgttct
    32941 tccccctgca taatgccaaa caaataaaat cgcgtttgtt ttgccgctcg ttccagccac
    33001 ccctgacgac gaatgcgata gtggtgacca ttatccccaa cggcattgat cagcacaaag
    33061 ccgagccagc atgccagtaa caaaaatccg caaacttcat gcaccgcaac cagactttta
    33121 accgcagttg cgcccaccat cgcaaagtga tttatcagcc cacttgccag caacaataca
    33181 aagagtaacg cattcgacca gtgccataat ctgaccgctt tgctgtaaag ataaaccttc
    33241 tcaccatgat cggtggctga tttcttcacg ccacgagcac gaagcaaggc gtgcagcccc
    33301 agcaccaacc acatcccaac cagcaatact ccggcaatga tcaaccacac cggccaaaat
    33361 tcgggagtga actgtggcac atagttcgct aactggctct gaaactgttc agcatgttgc
    33421 gacgggttca tacattttcc tttttaatca aatgttgacc gaaccggcga tacagatgcg
    33481 gttttccggc accaggtagc tgatattgat agtatttatt atcctgcaac cacgcctgaa
    33541 tttctgggct atcttcgcga ccaaagatca atgcgtgttc cggacaggcg ctgacgcaaa
    33601 tgggcggaaa gccttttgcc agtcgggact cagcgcagaa atcacattta tctgccactt
    33661 tagtgacagg gttaaggtag cgaaccaggt acggacaagc gccaatgcaa taactgcagc
    33721 cgatgcactg cgatttttct acccgcacga tcccctgctc atcacgccac gatgcccccg
    33781 tcgggcatac gtcgatgcac ggagcatctt cgcagtgctg acatgactgg cgaaaaaagt
    33841 gatactgcgt ttcgttgtcg ttatcggtga ctggaatgtg tgctatcgat aaacggcttc
    33901 cctgagcggg gacgtggtta gttttacggc atgcgcgggc gcaaatatta cagccattgc
    33961 accgtgactc gtcatgaatc atggcatacc gaacttcctt ttcttgcctc gtgttcgcta
    34021 acagagaaga cgcagaacca gtaaaaaaga ttactgttcc catccccaga acaaatttgc
    34081 gtcgagtgaa ggacatctga ttatccccta atgcagtggc tgcaactgtc caccctatcc
    34141 atgacatctc cctgaaattt caggggttct gaaactgctc tacctcgaac cattcgcata
    34201 agccataagc cttgccatag gtcataaatc gctcgctaaa caagtacaac accgccgtcg
    34261 atgactccac ctgccgtata tcggcatatt caggtgcaac atccatcgcg gcgatcgcgg
    34321 cttcaatctg cgcttcctgg aagtaatatg gcgcttgcat cagcatggcg actttgtaag
    34381 gacgaggata cgtctggcac tcaaaacgaa cggcgtgtgc aatggcgcga caaatgtcct
    34441 gctccacaac ttgcaaagac atcgccgcgt agttttcact catggcctgc gttgagtaat
    34501 agtaatcatc ctgacttccc gtcatacaag cgatatccgc gtagtctccg ttctgcttaa
    34561 tgctttccag caaaactgac agttcctctt ctgcaaccga atagggtggc gagagaaaca
    34621 cggtgcgcgc caccagctgc ccagccgcag aatgctggcg aatgaactca gccaggattt
    34681 cagcaggttc tggttcggtt accgtatcaa cggggtgacg catcccctgc aaccattgcc
    34741 gcttcgcctc gtcaacttcg ctgactttcg ctaacggcaa ttcatcctga tggttcatct
    34801 tacccattcc ttacgcaggc agtagattgt gtgctgccag atcggcggca atatcttcca
    34861 gccccagacg ctgcaatgtt tcgcgggttg gacaaccaag ctgtgggtcc cagcccattt
    34921 ctttgtagaa catcgtgagc gaagcatgca tatcgtcacg atccattttg tcagtacctt
    34981 cagtaaagac cgggatctgc ggatccttgt cgaaaaccca ggaacagata agatcgtgtt
    35041 cattacgcat atctttggtt tgcatcggtt ttaccgtgta ggcacgatgc aacgtaaaga
    35101 tacgctctgc agctaaatct aatttttcct gagtcatctc ttcgccggtg atcgctttga
    35161 agaatttggc ttcaagcgcc agatcgcccc ggtaattacg gcttttcagc ggcgaaacgg
    35221 tcattggcca gacccagttg cacagagtga cggcgttatg caaacagacc cgcaacagcg
    35281 accactttgc atatttaatt tttgcgtcat tgattggcgt gtagtttttg gtttcatcgt
    35341 aagcatcttc agaaccaaaa agttctttcg ccacttcacg ttgcagtttc aatggcaagc
    35401 cggaaccaat aaagttgata tgggtatgcg tcatgcagtc acggttgaac atacacttaa
    35461 caatggaacc gacctgcgct gacgcttcat tggcatggtg aaccggatag ccaaacggcg
    35521 accagagttt atttttcgcg tagccccagt actctttacc caaattccag cgttctgcga
    35581 tggcatatga accatcagcc aggtgactca gctcacccac acgatgcgcc agacggtagt
    35641 aaaaatcttt aatgaagtta acgtcacccg cttccagttg atcccagcga atttctgcat
    35701 actcttcagc tggcagaaca cgcttgaaca cacctttgct gtaacaataa gtaaagtcgc
    35761 gatgcaactg cccgtagtta caccataggc cgtagtcatc gaacaaattc agaccaacca
    35821 ggttaccaat cacacgacca tcgtctttat cttcaaaatc tttcggcccg ttcgggaaga
    35881 tggtggtatg gacaaagttt gctacacaag tgttaccacc tgtgctgggc acgccaaact
    35941 ctttcacccg aggaatattc atttgggtca tacaacggat cgggcaagag tggcagccgc
    36001 tcatttttac tgtgtatttc tctgccgccg gtcctaagtc aaaaacggat ttataggtac
    36061 gaaagccgac cgtattctga ttgcctggcg gaatttcacc cgtttcaatc ggaccacctt
    36121 cagccgcgcc ccaaaacagc cctttacgtg ctgtccagcg tgacttggga tctgaatact
    36181 ctgcccacga ttgtggcgta cttggcacga catggttgtt attcgcacca ataagttcag
    36241 tcatcatgta atcattgaga cgcttcatct cctgacgatc ggcaatgttg acccctttcg
    36301 tcccttcaac cgcaatcgct ttcaggtttt tcgaacccat tattgcgcca gttcccgcac
    36361 cgccgctgtg gttacggcta ttcaacatgc cagagagagg aataaggttt tccccagcct
    36421 gaccaatagc cgccacacag gtttccgggc tggtcaatcg acaaatttct tccgtcgttg
    36481 cgcgcgtccc ttttccccat aagaaatcgg ctttttccag gctaactttg tcatctttaa
    36541 tcttcagcca taccggtgat ttcgccttcc cttcgataat aatgacgtcg tatccagcga
    36601 atttcatttg cgctgcaaaa aagccaccca tatgggcatc gcgactaaat ttcctttggt
    36661 aaaagtagaa agtgaggtga tatttacgcg agaactacag ggggcaccag atccaggtaa
    36721 tgggccggta gcaaagacta atttattcgc ttcatcgaaa ggtttcgtgc ctagcggtac
    36781 ttcgtcatac ataattttgt agccgaagcc catgccaccg acaaaacttt taaacttact
    36841 ggaatcttcg agggtaatat ttcctgtcgt gagattgact cttaatatat tacctgtcca
    36901 accgttagcc atgatttttt cctttgcaag attcacacag taatatcttt ccactcgata
    36961 atttttaacg cccctgttgg gcaggcgttt gcgcattcac cgcataacac acatttcgag
    37021 gattttttac tttcggtatt tacggtggcc atcatccacg ggcatgccgt agtacaggcg
    37081 ctacagccaa tacaacgttt atgatcgacg gtaatacagc cttctttctg ctgccaggta
    37141 atcgcaccaa tcgggcagac gttcatgcat tgcggctctt tgcattgacg gcaggtgtcc
    37201 gcggtatagt tgagatcgcc atacaggccg ccgccagagc caaccccgtt gtcgccaaag
    37261 aaataattgc gatggatttt gatacgggag aagaatgtcc ctactgagcc atcgttgaag
    37321 ttggtacaag agatttcaca gcggtggcaa cccgtgcatc gcgctcgctg ggtcaccagc
    37381 acccctttgg gtgtatttat caaccctact gtgccgctat caatatcttc ctgtttgcaa
    37441 cccaaaagtg acagtaacgc aggggcaatt gttagaccag caaggccttt tcctgatata
    37501 cgcaagaatt ctaatcgcgt taagccaata tctaatagtg gacgatcaac cgggttcatt
    37561 tatttcgcat cctcttcgca aacacagtgc tgacaattca gcagcctgat attaagcatt
    37621 caactaatac gctgctgact ctgtccccaa caccagatat tttccctaac ccgatagtgt
    37681 atgagaaaag atttaacgcc gataaactaa tcctttatgg ttaggagaaa aaataatgtg
    37741 attattctga gcccttaaca ttgatcgtta tcaattaaaa ttacaaagca agaaattata
    37801 agcgaactga tatttattat catttgaaat aaatttaact taagcgacaa tcatttgtga
    37861 cgttcattgc agaaaataaa aataacatta tcgctataaa ttaaaataca taccgaaatt
    37921 cgtataattt cagcagttgt tatttagaat gattaactgg accgcaactg aagcaagaat
    37981 atcaggatga agcttaccgc ctcatcctga atggtcattt taccagtgcg ttaatgacgg
    38041 tttccatcgc ctgcttttcc agctcactgc gctgaatgcg tttattggct aatgctgtac
    38101 gtaatacgcc tgcgataaca atatgctttg gttcctgccc cagatcgcgc atttccagca
    38161 caactttacc gacaactctg cacatttctc ggtacagttc atcctctttt gtccgatttc
    38221 ccattcttta tacctattta tcatgccaa
//
LOCUS       DAJTSF010000029        37023 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000029 DAJTSF010000000
VERSION     DAJTSF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.029"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..175)
                     /locus_tag="OO335_001574"
     CDS             complement(<1..175)
                     /locus_tag="OO335_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR6002008.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OO335_001575"
     CDS             complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OO335_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6002009.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OO335_001576"
     CDS             complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OO335_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6002010.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OO335_001577"
     CDS             complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OO335_001577"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6002011.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OO335_001578"
     CDS             complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OO335_001578"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6002012.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OO335_001579"
     CDS             complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OO335_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6002013.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5179..5421)
                     /locus_tag="OO335_001580"
     CDS             complement(5179..5421)
                     /locus_tag="OO335_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6002014.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OO335_001581"
     CDS             complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OO335_001581"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6002015.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OO335_001582"
                     /gene_synonym="fabE"
     CDS             complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OO335_001582"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6002016.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8338..9312)
                     /locus_tag="OO335_001583"
     CDS             complement(8338..9312)
                     /locus_tag="OO335_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6002017.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9464..11404
                     /gene="csrD"
                     /locus_tag="OO335_001584"
     CDS             9464..11404
                     /gene="csrD"
                     /locus_tag="OO335_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6002018.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11709..12752
                     /gene="mreB"
                     /locus_tag="OO335_001585"
     CDS             11709..12752
                     /gene="mreB"
                     /locus_tag="OO335_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6002019.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12818..13921
                     /gene="mreC"
                     /locus_tag="OO335_001586"
     CDS             12818..13921
                     /gene="mreC"
                     /locus_tag="OO335_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6002020.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13921..14409
                     /gene="mreD"
                     /locus_tag="OO335_001587"
     CDS             13921..14409
                     /gene="mreD"
                     /locus_tag="OO335_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6002021.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14418..15011
                     /gene="yhdE"
                     /locus_tag="OO335_001588"
     CDS             14418..15011
                     /gene="yhdE"
                     /locus_tag="OO335_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6002022.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15001..16470
                     /gene="rng"
                     /locus_tag="OO335_001589"
     CDS             15001..16470
                     /gene="rng"
                     /locus_tag="OO335_001589"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6002023.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16538..20338
                     /gene="yhdP"
                     /locus_tag="OO335_001590"
     CDS             16538..20338
                     /gene="yhdP"
                     /locus_tag="OO335_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6002024.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20494..21939
                     /gene="tldD"
                     /locus_tag="OO335_001591"
     CDS             20494..21939
                     /gene="tldD"
                     /locus_tag="OO335_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6002025.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OO335_001592"
     CDS             complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OO335_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR6002026.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23179..23382
                     /locus_tag="OO335_001593"
     CDS             23179..23382
                     /locus_tag="OO335_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6002027.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23390..24322
                     /gene="aaeA"
                     /locus_tag="OO335_001594"
     CDS             23390..24322
                     /gene="aaeA"
                     /locus_tag="OO335_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR6002028.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24328..26295
                     /gene="aaeB"
                     /locus_tag="OO335_001595"
     CDS             24328..26295
                     /gene="aaeB"
                     /locus_tag="OO335_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6002029.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26387..26659
                     /locus_tag="OO335_001596"
     CDS             26387..26659
                     /locus_tag="OO335_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6002030.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO335_001597"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO335_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6002031.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO335_001598"
     CDS             complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO335_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6002032.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28248..29186
                     /gene="mdh"
                     /locus_tag="OO335_001599"
     CDS             28248..29186
                     /gene="mdh"
                     /locus_tag="OO335_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6002033.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO335_001600"
     CDS             complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO335_001600"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6002034.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="OO335_001601"
                     /pseudo
     CDS             complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="OO335_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31906..32304)
                     /locus_tag="OO335_001602"
     CDS             complement(31906..32304)
                     /locus_tag="OO335_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6002035.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32498..33625
                     /gene="zapE"
                     /locus_tag="OO335_001603"
     CDS             32498..33625
                     /gene="zapE"
                     /locus_tag="OO335_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6002036.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33844..34272
                     /gene="rplM"
                     /locus_tag="OO335_001604"
     CDS             33844..34272
                     /gene="rplM"
                     /locus_tag="OO335_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6002037.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34288..34680
                     /gene="rpsI"
                     /locus_tag="OO335_001605"
     CDS             34288..34680
                     /gene="rpsI"
                     /locus_tag="OO335_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6002038.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35075..35713
                     /gene="sspA"
                     /locus_tag="OO335_001606"
     CDS             35075..35713
                     /gene="sspA"
                     /locus_tag="OO335_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6002039.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35719..36216
                     /gene="sspB"
                     /locus_tag="OO335_001607"
     CDS             35719..36216
                     /gene="sspB"
                     /locus_tag="OO335_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6002040.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36259..>37023)
                     /gene="dcuC"
                     /locus_tag="OO335_001608"
     CDS             complement(36259..>37023)
                     /gene="dcuC"
                     /locus_tag="OO335_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6002041.1"
                     /translation="SCVIISVAISHFFVQRAFDKKDKNINHEQAEQKTLDNVPPLYYA
                     ILPVMPLILMLGSLFLAHVGLMQSELHLVVVMLLSLTVTMFVEFFRKHNLRETMDDVQ
                     AFFDGMGTQFVNVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSGLGGIGVMIIMALVI
                     AICAIVMGSGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATTLARAVSPITAVVVV
                     TSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         8948 a         9249 c         9751 g         9075 t
ORIGIN      
        1 ttctgtcctg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
       61 tattacggca tttgtctgtt tctgttgtca gcagttcaat ccactgcatc agtaaagtat
      121 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      181 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      241 actaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccggct
      301 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcggagta
      361 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
      421 accaatgaat tttcgtccgc tggcgatggc tacggcaccg gtagtaaagc taccggcaaa
      481 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
      541 tttcggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgcggaaa
      601 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
      661 atcgatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
      721 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
      781 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
      841 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
      901 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
      961 aaacagatct tctttccagg cttcgatcag accatcaaaa tttttaccga tgttatacgg
     1021 tgggtcggca aagagcagat cgacgctttc agtaggtagc tttttaagtt cggcaaaggc
     1081 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1141 catggtcact ccctttgtga cacctataaa aaaggcgctt ccccatgccg agtagcgcct
     1201 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1261 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     1321 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     1381 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     1441 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     1501 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     1561 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     1621 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     1681 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     1741 agcaagcgct taacctctgc caaaggcagt gggggcagca actccccagt gtccagataa
     1801 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     1861 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     1921 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     1981 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2041 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2101 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2161 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     2221 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     2281 atttctttcg gagcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     2341 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     2401 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     2461 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     2521 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     2581 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     2641 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     2701 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     2761 tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     2821 cgtcacccta cggcgatgcg aaggtcaatt cttacgaccg gtaatacggc accactcttc
     2881 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     2941 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaactggca ggacgctgat
     3001 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3061 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cgccattacg
     3121 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3181 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3241 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     3301 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     3361 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     3421 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcgattt tatgcgcgaa
     3481 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     3541 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     3601 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     3661 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     3721 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     3781 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     3841 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     3901 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     3961 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4021 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4081 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4141 tctttaatga tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca
     4201 gccataggtg cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac
     4261 gtcgggatca ccagatctgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     4321 gccaggtgca taccgaacat caggattgcg actataatcg taccgatgat gatcccccgg
     4381 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     4441 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     4501 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     4561 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     4621 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     4681 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     4741 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     4801 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     4861 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     4921 gaaagccaga cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta
     4981 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagagtc
     5041 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5101 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5161 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5221 aaatttcacc atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat
     5281 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcgtctac
     5341 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgggcct ctttatgggc
     5401 ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     5461 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     5521 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     5581 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     5641 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     5701 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     5761 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     5821 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     5881 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     5941 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6001 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6061 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgttcgc cgatgtagcg
     6121 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6181 ctgcatggag cagtcacgtt ccgccagata gatagcgttg ccctgaccgt cagccagtac
     6241 ctgaatctcg acgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct
     6301 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     6361 gcgcactacg cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata
     6421 accaatgcgt ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga
     6481 accaggtacg caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     6541 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     6601 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     6661 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taacttttta ctgacggagc
     6721 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgtgct
     6781 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     6841 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     6901 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     6961 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7021 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7081 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7141 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7201 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7261 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     7321 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     7381 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     7441 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     7501 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     7561 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     7621 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     7681 gaatataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     7741 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     7801 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     7861 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     7921 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     7981 ccaactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat
     8041 cagattattc accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg
     8101 caaagcacca taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt
     8161 atcaatgcgc atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg
     8221 tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa
     8281 ccgattgaat aatgatgtca aaatgagttc cctctcttat tattccttgc taaatggtta
     8341 gttaaccttc accagcgtgc gaccctggat ctggttatta atgatggcct cggcaaagtt
     8401 cggtgcctct gacagagata tctcttttgc cgcctgggta tagaatgatt ccggtaaatc
     8461 ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac
     8521 cccttgcaaa cggacattac gcagaataaa tggcatgacc gtggttggca gagtaaaacc
     8581 acccgccaga ccacaggccg ccacgcagcc gccgtaattc atttgcgcca gcacttttgc
     8641 cagcactttg tcgccaacag tgtcaattgc cccagcccag acttgttttt ccagaggacg
     8701 ggattcggca aactcatcac gagggagaat accgctggca cctaaacttt tcagatattc
     8761 atgggtactt tcgcgaccgg aaacggcaac gacctgataa cccaacttat gcagcagcgc
     8821 cacggcggta ctgccgacgc caccactggc acccgtcacg acaatctcac cgtcctgcgg
     8881 gcgaacaccg gcatcttcca gcgccatcac acacagcatg gcggtaaaac cggcagtacc
     8941 gataatcatt gctttacgcg cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt
     9001 cactcgcgcc tgctccgcca gcccacccca gtggttttca ccaacgcccc agccagtgag
     9061 taacacctcc tgaccggcat gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa
     9121 atcgatccca ggaatcatcg gaaaattacg gatgattttt cccttaccgg taatcgccag
     9181 cgcatcttta tagttcaggc tcgaccagtg aacatcgacc gtgacatcgc cctccggcag
     9241 gcgacgttcg tccagagtct gtactgatgc gagagttttg ccgtcctgct gttctaaaag
     9301 taacgcctgc ataagtggtc ctcatgtgca tgatggattg gaaaataatt tctgaagact
     9361 atactcgcta atggaaaagc aaagccgatg aagcgcaata aattgcgaga taaatctgat
     9421 ttgctagtat gcccgcttcc tcactatcgg agttaacaca aggatgagat taacgacgaa
     9481 attttcggcc tttgttacgc tgctcaccgg gttaacaatt tttgtgactt tgctgggctg
     9541 ttcgctaagt ttctacaacg ccattcagta taagtttagt catcgcgttc aggcggtggc
     9601 gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac
     9661 cgaattaatg gtgtcagcag atatcgttcg tgtagacctg ctgcatggtg ataaacaggt
     9721 ttataccctg gccagaaatg gtagttatcg tccggttggc accagcgatc tgtttcgtga
     9781 actgagcgtt ccgttgataa agcatccggg gatgtcgttg cgtctggttt atcaggatcc
     9841 gatgggcaac tatttccatt cgttgatgac caccgcgccg ctcacggggg cgattggctt
     9901 tatcattctt atgctctttc tggcggtacg ctggttacaa cggcaacttg ccgggcaaga
     9961 attgctggaa acccgggcta ctcgtatctt aaacggtgag cgtggctcta atgtgttggg
    10021 aaccatctat gaatggccgc ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat
    10081 tcagaacgca cgcgaacaac acagccgtct tgatacgctg atccgctctt atgccgcaca
    10141 ggacgtgaaa accggcctca ataaccgact ctttttcgat aatcagttag caacgttact
    10201 ggaagatcag gagaaagtag gtacccacgg gatcgtgatg atgattcgtc tgccggattt
    10261 caatatgttg agcgatacct gggggcacag ccaggttgaa gaacagttct tcactctgac
    10321 gaatctgctg tcgacattta tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg
    10381 cagtgatttt gctgcgctgt taccgcaccg gacgttaaaa gaggcagaga gcatagccag
    10441 tcagttaatc aaagccgtcg ataccttgcc gaacaataaa atgctcgatc gcgacgatat
    10501 gatccacatt ggtatctgtg cctggcgtag tggtcaggat accgagcagg taatggaaca
    10561 tgcagagtct gccacgcgta atgcgggatt gcagggtggc aatagctggg ctatttatga
    10621 tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat
    10681 gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg gttactcgcg aaggtcaggt
    10741 tcatcatcgc gaactcatgt gccgcatctt cgatggtaat gaagaggtta gctcggcgga
    10801 gtatatgccg atggtcttgc agtttggctt atcggaagag tatgaccgtc tgcaaatcag
    10861 ccgtcttatt ccactattgc gttactggcc agaggaaaat ctggcgattc aggttaccgt
    10921 tgagtcgctg attcgtccgc gttttcagcg ttggctgcgc gatacgttaa tgcaatgtga
    10981 aaaatcacaa cgaaaacgca taattattga acttgcagag gccgatgtag gtcaacatat
    11041 cagtcgttta caacctgtta ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa
    11101 ccaggctggt ttgacgctgg taagcaccag ttggatcaaa gaacttaatg ttgagttact
    11161 caagctccat ccggggctgg tcaggaacat tgagaagcga acggagaacc agctgctggt
    11221 tcaaagcctg gtggaagcct gctccgggac cagcacccac gtttacgcca ccggcgtgcg
    11281 ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt acaggcgggc aaggggattt
    11341 tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt
    11401 ttaacctgcc gtttaatccg ttttcacgta gaataacgcg cgctgcgtct catgggagtg
    11461 tgcttgtctg ctcgccagat tgttgcagca catatgcaga tgaatgacct tacgcggttg
    11521 caaacaggcg aggaatgctg ctgatgcatt aagcctttct ggactcaggc agagatttgt
    11581 aacaaaggaa acgaactgca ctaattttca ccgtagcaga tgatttttgc gccttgtcgc
    11641 tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc gccccagctt tcaggattat
    11701 cccttagtat gttgaaaaaa tttcgtggca tgttttccaa tgacttgtcc attgacctgg
    11761 gtactgcgaa taccctcatt tatgtaaaag gacaaggcat cgtattgaat gagccttccg
    11821 tggtggccat tcgtcaggat cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg
    11881 acgcgaagca gatgctgggc cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag
    11941 acggcgttat cgccgacttc ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag
    12001 tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg
    12061 cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag
    12121 tcttcctgat tgaagaaccg atggctgccg caattggtgc tggcctgccg gtttctgaag
    12181 cgaccggttc tatggtggtt gatatcggtg gtggtaccac tgaagttgct gttatctcct
    12241 tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta
    12301 tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg tgaagccacc gcagaacgta
    12361 tcaagcacga aatcggttcg gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg
    12421 gccgtaacct ggcagaaggt gttccacgcg gttttaccct gaactccaac gaaatcctcg
    12481 aagcactgca ggaaccgctg accggtattg tgagcgcggt aatggttgca ctggaacagt
    12541 gcccgccgga actggcttcc gacatctccg agcgcggcat ggtgctcacc ggtggtggcg
    12601 cactgctgcg taaccttgac cgtttgttaa tggaagaaac cggcattcca gtcgttgttg
    12661 ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca
    12721 tgcacggcgg cgacctgttc agcgaagagt aatcggatgc aggcagggga agcgtctgtt
    12781 taccctgcct ggtctgatac gagaatacgc ataacttatg aagccaattt ttagccgtgg
    12841 cccgtcgcta cagattcgcc ttattctggc ggtgctggtg gcgctcggca ttattattgc
    12901 cgacagccgc ctggggacgt tcagtcaaat ccgtacttat atggataccg ccgtcagtcc
    12961 tttctacttt gtttccaatg ctcctcgtga attgctggat ggcatatcgc agacgctggc
    13021 ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa
    13081 cagtgaactg ctgatgcttg gacaatacaa acaggagaac gcgcgtctgc gcgagctgct
    13141 gggttccccg ctgcgtcagg atgagcagaa aatggtgact caggttatct ccacggttaa
    13201 cgatccttat agcgatcaag ttgttatcga taaaggtagc gttaatggcg tttatgaagg
    13261 ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac
    13321 cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa
    13381 cgatatccgc gtaattgcag ccggtaacgg ttgtacggat gatttgcagc ttgagcatct
    13441 gccagcgaat acggatattc gtgttggtga tgtgctggtg acctccggtc tgggcggtcg
    13501 tttcccggaa ggctatccgg tcgcggttgt ctcttccgta aaactcgata cccagcgcgc
    13561 ttatactgtg atccaggcgc gtccgactgc aggactgcaa cgtttgcgtt atctgctgct
    13621 gctgtggggc gcggatcgta acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt
    13681 tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg ccttcgccag acgcgatggg
    13741 gccaaagtta cctgaaccgg caacggggat cgctcagccg actccgcagc aaccggcgac
    13801 aggaaatgca gctactgcgc ctgttgcgcc gacacagccc gctgctaatc gctctccaca
    13861 aagggctacg ccgccgcaaa gtggtgctca accgcctgcg cgtgcgccgg gagggcaata
    13921 gtggcgagct atcgtagcca gggacgctgg gtaatctggc tctctttcct cattgcgctg
    13981 ttgctgcaaa tcatgccctg gccggataac ctgattgttt tccggccaaa ctgggtgtta
    14041 cttatcttgt tgtattggat cctggccttg ccccatcgcg taaatgtggg cacaggtttt
    14101 gtgatgggtg ccatactgga tctgatcagc ggctcgatgc ttggcgtacg cgtattggcg
    14161 atgagcatca ttgcttacct ggtggcgctg aaataccagc ttttccgcaa cctcgcatta
    14221 tggcagcagg cgctggtcgt catgttgctt tcgctggtgg tggatattat tgttttctgg
    14281 gcagagtttt tagtgattaa cgtctctttc agaccggaag tgttctggag tagtgtagtc
    14341 aatggggtgc tctggccgtg gattttcttg ctgatgcgta aagtccgtca gcagtttgca
    14401 gtgcaataaa ggtttctatg acttctctgt atttagcttc cggttctccg cgtcgtcagg
    14461 agttacttgc gcaacttggc gtgacctttg aacgtattgt tacgggcatt gaggagcagc
    14521 gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct ggcacgcgag aaagcacagg
    14581 cgggtgtcgc gcaaacggca caggatctcc cggtgctggg tgcggatatt atcgttatcc
    14641 tgaacggaga agtgctcgag aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca
    14701 aattatcggg tcagacccat caggtgatga cggcagtggc gttggccgac agccagcaca
    14761 ttctcgattg cctggtggtc accgatgtga ctttcagaac gttaacagac gaagacatcg
    14821 cgggctatgt cgccagcggt gaaccgttag ataaagcagg tgcatacggt attcaggggc
    14881 tgggtggctg ttttgtcagg aagataaatg gcagctatca cgccgtagtc ggcttaccgc
    14941 tggttgaaac gtatgaatta ttaagtaatt ttaacgcact gcgtgagaaa agggataaac
    15001 atgacggctg aattgttagt aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat
    15061 ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat
    15121 atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt
    15181 gggctggata aagccgcgtt tcttcatgca tccgacatca tgccgcatac cgaatgtgtg
    15241 gcgggtgaag aacaaaagca attcacggtg cgcgacatct cggaactggt tcgtcagggg
    15301 caagatctga tggtgcaggt ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc
    15361 accgatatca cgctgccttc tcgctatctg gtatttatgc caggggcttc tcacgttggg
    15421 gtttcccaac gaattgaaag cgaatcagaa cgtgaacgtc tgaaaaaagt ggtcgcagag
    15481 tattgcgacg agcagggcgg gtttatcatc cgcaccgcag cggaaggggt tggcgaggct
    15541 gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa
    15601 aagcgcccgc agacccgtta tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg
    15661 cgtgattttg ccgacgccga actggaccgc attcgcgttg actcacgcct gacttacgaa
    15721 gcattgctgg agtttacctc ggagtacatt cccgagatga ccagcaagct ggagcattac
    15781 accggacgcc agccgatttt cgatctcttt gatgtcgaaa acgaaatcca gcgagcgttg
    15841 gaacgcaaag tagaactgaa atccggtggt tatctgatta tcgaccagac cgaagcgatg
    15901 accaccgtgg acatcaatac cggagcgttt gtcggtcatc gcaatctgga cgacactatt
    15961 ttcaatacca atattgaagc gacgcaggct atcgctcgcc agttacggtt gcgtaatctg
    16021 ggcgggatta tcattattga tttcatcgat atgaataatg aagatcaccg ccgccgcgtg
    16081 ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga aaaccagcgt taatggtttt
    16141 tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta
    16201 ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta
    16261 tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg cttacgactc cgaccgtttc
    16321 ctggtctatg cttctccggc agtagccgaa gccttgaaag gcgaagagtc acactcgctg
    16381 gcggaagtgg aaattttcgt tggcaaacag gttaaagtac aaattgaacc gctctataac
    16441 caggagcagt ttgacgtcgt aatgatgtaa acagatgctg ggccgccatc cggcaaaggg
    16501 tttttgagtc acatttttag cagacaagga gtgacgggtg aggcgattgc cggggatttt
    16561 actgcttact ggagccgcgc tcgttgtgat cgctgccctg ctggttagcg gcctgcgtat
    16621 tgctttaccg catcttaacg cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac
    16681 tggcatgccg gtagaagcca gtcagctctc agccagctgg cagaattttg gcccgacgct
    16741 tgaagcacac gacatccgtg cagaactaaa agatggcggc gaattttcgg ttaaacgcgt
    16801 tactctggcg ctggatgtct ggcagagcct gttacatatg cgctggcagt ttcgcgacct
    16861 cactttctgg cagctgcgct ttcgcaccaa cactcctatc accagcggtg gtagcgatga
    16921 cagtctggaa gccagtcaca tcagcgatct gtttcttcgt caatttgacc atttcgatct
    16981 ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag cgcgccgagc tggcgatccc
    17041 acaactcacc tggctgaacg atccacgtcg acaccgtgcg gaaggcctgg taagcctctc
    17101 cagccttacc ggacagcacg gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg
    17161 gttgttaagc aatggtcgcg tctggctaca ggcggatgac atcgacctga agccgtggct
    17221 cggtaaatgg atgcaggaca atattgctct ggaaacggca cagttctccc ttgaaggctg
    17281 gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc
    17341 cagctggttg ggcgagaagc aaacgcatac gctgtcggtg gataatctga ccgcgcatat
    17401 tacgcgtgaa aatccgggct ggcagttctc tattcccgat acacggatca cgatggacgg
    17461 caaaccctgg ccgagcggag cattgacgct ggcctggata ccggaacagg acgttggcgg
    17521 caaagacaat aaacgcagtg acgaactccg gattcgcgct agtaatctgg agctggcagg
    17581 cctggagggc atacgcccgc tggccgcgaa actttcacct gcactgggtg atgtttggcg
    17641 ctccacacaa ccgagcggca agattaacac tctggcgctg gatatcccgc ttcaggcggc
    17701 agacaagacc cgttttcagg catcgtggag cgatctggcc tggaagcaat ggaaattatt
    17761 accgggtgcg gaacacttct ccgggacgct ttccggcagc gttgaaaatg gtttgcttac
    17821 cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta ttccgtgcgc cactggaaat
    17881 cgccgacggc caggcaacta taagctggct gaacaatgac aaaggtttcc agctggatgg
    17941 gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca
    18001 acctgctaac gatgaaccct ggctgggtat tctggctggc atcagtaccg atgatggttc
    18061 acaagcctgg cgctatttcc cggaaaactt gatgggtaaa gacctggttg attatttaag
    18121 tggtgcgatt cagggcggtg aagcggataa cgcgacgctg gtttatggtg gcaatccgca
    18181 actcttcccc tataaacaca acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc
    18241 gaagtttgcc ttccagccgg actggcctgc attaactaac cttgatattg aactggactt
    18301 tattaacgac ggtttatgga tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag
    18361 taatctcacc gcagtgatcc ctgactactc aaaagaaaaa ctgctgattg acgctgacat
    18421 taaaggtccg ggtaaagccg ttggccctta ctttgatgag acaccgctga aagattctct
    18481 gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg aatgctcgct tacatcttga
    18541 tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag
    18601 tctgtttatc aaaccactcg acagcatcct gaaaaatttg agcggtaaat tcagctttat
    18661 caatggcgat ctgcaaagtg aaccactgac agcaagctgg tttaatcagc cgttgaacgt
    18721 ggatttttcc accaaagaag gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa
    18781 ctggcaaccg gcgaaaaccg gcgttctgcc tgaagcggtg aacgaagcat tgagtggcag
    18841 cgtggcgtgg gatggtaaag tgggcattga tctgccttat catgctggcg cgacctataa
    18901 cgtagagctg aacggcgatc tgaataatgt gagtagtcac ttaccttcac cgttagccaa
    18961 acctgcgggt gaaccactag cggtaaacgt taaggttgat ggcaatctca acagctttga
    19021 attaaccgga caggctggtg cggataacca tttcaatagc cgctggttgc ttggtcaaaa
    19081 gctgacgctc gatcgtgcta tttgggcggc agacagtaaa acgctcccgc cgttgccgga
    19141 acaaagtggc gttgaactta atatgccgcc gatgaatggt gccgagtggc tggccctgtt
    19201 ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt ttcccacaac acataacgtt
    19261 acgtacgcct atgttgtcac tgggaaatca gcaatggaat aacctgagta ttgtttcgca
    19321 accgacggca aatggcaccc tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc
    19381 gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat ctttattaca acccgagcgt
    19441 ggcgaaaact cgtggtgatt caacgccgtc atcacctttc ccgacaacgg agcgcattaa
    19501 cttccgtggc tggccggacg cacaaatacg atgcacagag tgctggttct gggggcaaaa
    19561 attcggtcgc attgacagtg atatcaccat ttctggcgat acgttaacgc tgaccaatgg
    19621 actgattgat actggtttct cgcggcttac tgccgatggt gaatgggtta ataatccggg
    19681 gaatgaacgt acctcgctga aaggaaaact gcgtgggcag aaaattgatg ccgccgcaga
    19741 attttttggt gtcacgacgc ccatacgcca gtcgtcattt aatgtggatt acgatttaca
    19801 ctggcgcaaa gcaccctggc agccagatga agcgacgttg aatggcatca ttcatactca
    19861 actgggtaaa ggcgaaatta ccgaaatcaa taccggacat gccgggcaat tgctgcgctt
    19921 attgagcgta gatgccctga tgcgtaagct gcgttttgat ttcagagaca cttttggcga
    19981 agggttctat tttgactcca ttcgcagcac cgcgtggatt aaagacggcg ttatgcacac
    20041 cgacgacacg ctggtggatg gcctggaggc ggatatcgcc atgaaagggt cggtaaatct
    20101 ggtacgccgc gacctgaata tggaggcggt tgtcgcacca gagatttctg cgacggtggg
    20161 cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg gcagtgtttg ccgccagtaa
    20221 agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc tatcacattt cgggtccgct
    20281 ggacgatccg caaatcaacg aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt
    20341 tgacgagggc gcgtaattgc cccaatctca tatgataatc gttgccaaag gccaacgagc
    20401 cggaacataa ctgtaggccg gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc
    20461 ctctactgca gtaactaacg agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa
    20521 ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg tcaactggcc
    20581 gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga atcctgggtt
    20641 ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt tggcgtgcgt
    20701 gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct gctggcgctg
    20761 gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg caaagtacag
    20821 acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct gcaaagcatg
    20881 agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgctcg cgaagcggac
    20941 aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat tctggttgcg
    21001 gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc cgtgagcgtt
    21061 ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg tcgttttggt
    21121 tatgagttct tccttgccga tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa
    21181 gcagtgcgta tggcgctggt caatctttct gccgttgctg caccagcggg caccatgccg
    21241 gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg
    21301 gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg ggagctggtg
    21361 gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg
    21421 gcgattgatg atgaaggtac gccaggccag tacaacgtgc tgattgagaa cggcattctg
    21481 aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc gactggcaac
    21541 ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac ctatatgctg
    21601 ccgggtaaat cgaccccgca agaaattatt gaatccgttg agtacggtat ctatgcaccg
    21661 aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc cacttcagaa
    21721 gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac gttgattggt
    21781 tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa actggataac
    21841 ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg ccagccaacg
    21901 ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcatta ttttcagaag
    21961 gataattaat ctttctacgt gcacgaacgg tcccctcgct cctctggggt tagggtgagg
    22021 ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa tatgaattac ttctctttcc
    22081 cgcgcccgtg catctcctga aacaatttac cgacctcaac aaaataatcc gtcagcgagt
    22141 tgatcacgac ctgtaccttc agcggcagct tatctttttc ggtatataac gcataaaccg
    22201 ggcgtggatc tgactgataa cgcggcagca ggatctccag ctccccacga ttgatctcgt
    22261 tgatcaccca catcagcggc acgtaggcga tcccggcacc cgccgttagc cagcgcacca
    22321 gcgtcatcgg atcattagtc acaaatctcc cttgcgggat caggcgagtc gagatccctt
    22381 ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct gtattcaagc catgaatggc
    22441 tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc
    22501 acaccaccat tggcatcgcg ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc
    22561 cgacgcggat caccacatcc agaccgtcgg caatcaggtc gggggctgga attccggtaa
    22621 ccagattggc gctcaaacct gggtattctt tcagcatttt ggctgtcagc ccggcgagaa
    22681 cattttgtgc catagttgaa gaacagccaa tgcgtagcgt cccgatgggg gtgttattga
    22741 aggcatacag ttgctcatga acatcctgca cttcatgaag catacgacgg cagccctggt
    22801 agtaaattct accggcttcg gtcaggccaa tgctgcgtgt gctacggttt aacagcttta
    22861 cctgcaactc atcttccagt tttgacaccg tctgactgat ggacgaaacg ctcatctgta
    22921 gctgtctggc ggcggcggta aaagagccaa attcaactac tttggcaaac accgacatgc
    22981 gttttagtcg ttccattatt cactctgact taaaagtgat ttagatcaca taatatagat
    23041 aacagcataa cagttacgct aatatattaa atatcaatct acagcaatgt tgctctcgcc
    23101 cggctttcca tgcccttctc tgcggcgaca gatgctgaaa ataataacgc ctgctctctc
    23161 tatacaacca aggtcaacat gagtctgttt cccgttatcg tggtgtttgg gctgtccttc
    23221 ccaccgatat tttttgaatt gcttttatca ctggcgattt tctggctggt gcgccgggta
    23281 cttgtgccaa caggtatcta cgactttgtc tggcatccgg cgttgttcaa caccgcgctc
    23341 tattgctgct tgttttattt gatatcgcga ctgttcgttt gaggttgaag tgaaaacact
    23401 aataagaaaa ttctcccgta cggccatcac ggtcgtatta gtcattctgg ccttcatcgc
    23461 aatttttaat gcctgggtct attacaccga atccccctgg acgcgtgacg cgcgctttag
    23521 cgctgacgtc gttgcgatcg cgccggacgt ttctggactc attacccagg tgaatgttca
    23581 tgataaccag ctggtgaaaa aaggacagat actgttcacc atcgaccagc cgcgctatca
    23641 aaaggcgctt gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa
    23701 acgccaggag gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat
    23761 cgaccaggct aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac
    23821 ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc cgagcgccag cagatggctg
    23881 ggtgaccaac ctcaacgtct ataccggtga gtttattact cgaggatcaa cggcggttgc
    23941 gctggtgaaa cagaactcct tctatgtact ggcctatatg gaagaaacta agctggaagg
    24001 ggtgcgtccg gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg
    24061 gactgttgat agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg
    24121 gatggcgacc atcgactctg accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg
    24181 tattcgtctc gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt
    24241 ggtcactggc aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca
    24301 tcgcctgcgt gagtttggtt aatcacgatg ggtattttct ccattgctaa ccaacatatt
    24361 cgctttgcgg taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc
    24421 cagctggaaa cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc
    24481 tttgctgcgg gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc
    24541 atcggcacat ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca
    24601 ccattattga tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg
    24661 ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg gttataccgc gctgatcatt
    24721 gtgatcacca ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc
    24781 gagatcgtta tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg
    24841 atcaaacaag aagtagatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg
    24901 caactctgta tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg
    24961 cgacgcacca cggcactaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg
    25021 gcgcgggcca atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa
    25081 tcctgcgaaa cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc
    25141 gaattttttg acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg
    25201 cggagagtta tcgcctggac tggggaacgg gaaacgcctg tcaccattta tagctgggtc
    25261 gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac
    25321 gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa aagtagagtc agccgaacgt
    25381 catcatgcaa tggttaactt ctggcgaacc acactttcct gcattctggg cacgcttttc
    25441 tggctgtgga cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg
    25501 tcactggcaa tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctatgggacg
    25561 ctggccgcgc tgccgttagg gctgctctac tttttggtga ttatccctaa tacccaacag
    25621 agcatgttgc tgctgtgtat tagcctggca gtgctgggat tcttcctcgg tatagaagta
    25681 cagaaacggc gactgggctc gatgggggca ctggccagca ccataaatat tatcgtgctg
    25741 gataacccga tgactttcca tttcagtcag tttctcgaca gcgcattagg acaaatcgtc
    25801 ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact
    25861 ggacgtgtac tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg
    25921 gcacgtcgta aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat
    25981 aagttcccag gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcgcaccag
    26041 cgcctgcgtg atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg
    26101 cgccgcacag cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt
    26161 ggccagttgc tggaagaact ggaaatctac caggaaaagc tacgcatctg gcaagcgcca
    26221 ccgcaggtga cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg
    26281 ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt
    26341 agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatgc
    26401 ctttgatttt gatgagattg agagtcagga ggatttttat cgtgacttta gccaaacctt
    26461 tggtctggct aaagataagg tacgcgatct cgactcacta tgggatgtgt taatgaacga
    26521 tgtcctgccg ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg
    26581 ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg
    26641 ttttaatgtt cgtcattagc gcaaaaaaaa gcccacgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagtatacac taattattga
    27061 actaattaat accacactaa ctgtttcagt tgatactaag tgttaaaaat gtgaatttta
    27121 ataacaaaaa agtataaaaa acacggttat ttattattaa ttatagtatt ttcaattaga
    27181 taatgaattt tgcataatgt gcttttttta ccgtatgtat tcattttgtg aatgatgtgt
    27241 cgcggtaaaa cgcactattc gttaaatgta tcgcaggaga aagaagggga atgagaggga
    27301 taagcaacat tttccccgcc gccagaagcg acggggcaga gattaaagct cctggtcgaa
    27361 cagctctaaa atcgcttcgt acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa
    27421 gatggtgtca tcgccagcga tggtgcccag aataccttct gctttgccca gtgagtccag
    27481 caggcgagca attaactgcg ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt
    27541 gtagtcgata tccagcacca gattcttcaa tggactggag gtggttggta cacccagttc
    27601 agctggcagg cagtaaacca tttccatttt ggcattgcgt gtacgtacag caccaaactt
    27661 ggtcaacatc cgcgagactt tagactgatt aatattgtca aagccttgct cctgcaacgc
    27721 ggcgacgatt tcgccctggg agctaaattt ctcttcttta agtaatgctt taaatgcttt
    27781 aactagttct tcttgcttag ccgagcttcg cataagtcac ccgatatggt ggttgataca
    27841 acattattgt gcatacagat gaatttttat gcaaacagtc agccctgaag aaggctgaaa
    27901 taatgttatg aaagagcggg attttatcaa atttcgttat tgagaaacat gcctgcgtca
    27961 cggcatgcaa attctgttta aaagtaaatt aattgttatc aaattgatgt tgttttggct
    28021 gaacggtagg gtatattgtc accacctgtt ggaatgttgc gctaatgcat aagcgactgt
    28081 taattacgta agttaggttc ctgattacgg caattaaatg cataaacgct aaacttgcgt
    28141 gactacacat tcttgagatg tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg
    28201 cagcggagca acatatctta gtttatcaat ataataagga gtttaggatg aaagtcgcag
    28261 tcctcggcgc tgctggcggt attggccagg cgcttgcact actgttaaaa acccaactgc
    28321 cttcaggttc agaactctct ctgtatgata tcgctccagt gactcccggt gtggccgtcg
    28381 atctgagcca tatccctact gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc
    28441 cggcgctgga aggcgcagat gtcgttctta tctctgcagg cgtagcgcgt aaaccgggta
    28501 tggatcgttc cgacctgttt aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag
    28561 ttgcgaaaaa ctgcccgaaa gcgtgcattg gtattatcac taacccggtt aacaccacag
    28621 ttgcaattgc tgctgaagtg ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg
    28681 gcgttaccac gctggatatc attcgttcca acacctttgt tgcggaactg aaaggcaaac
    28741 agccaggcga agttgaagtg ccggttattg gcggtcactc tggtgttacc attctgccgc
    28801 tgctgtcaca ggttcctggc gttagtttta ccgagcagga agtggctgat ctgaccaaac
    28861 gtatccagaa cgcgggtact gaggtggttg aagcgaaagc cggtggcggg tctgcaaccc
    28921 tgtctatggg ccaggcagct gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg
    28981 aacaaggcgt tgtcgaatgt gcctacgttg aaggcgacgg tcagtacgcc cgtttcttct
    29041 ctcaaccgct gctgctgggt aaaaacggcg tggaagagcg taaatctatc ggtaccctga
    29101 gcgcatttga acagaacgcg ctggaaggta tgctggatac gctcaagaaa gatatcgccc
    29161 tgggcaaaga gttcgttaat aagtaattga ttagcgaata ataaaaaacc ggagcacaga
    29221 ctccggtttt tgttttgagc actcgactta attggttgcc ggatattcct gaatggtgac
    29281 ctgcagcgtt aactgcttat catcacgcat cactacaacc gggatcaccg aaccagggcg
    29341 aatttccgcc acctgatcca tcgtctccag agcagagatg gccggtttgt tatccaccga
    29401 aataatcaga tcgttgacct gaatacccgc attcgccgct gggccgtcag gtgacacttc
    29461 attaaccacg atcccttgca gttgatctat accaccgctc tgcgcgtgca gtggtgcgat
    29521 ctcgcgtccg ccgataccaa tgtagccgcg gatcacgcga ccatcgcgga tcagcttatc
    29581 cataattttg gttgctaact ggaaaggaat cgcaaagccg ataccttccg gcgtttcgcc
    29641 atcgttactc ttatcaaacg acagcgtgtt aatgcccatc agttctccca gcgagttcac
    29701 cagcgcgccg ccagagttac cgtggttaat ggaagcatcg gtttgtagga agttttgccg
    29761 cccggtcggg ttcagaccga ttcgacccgt ggcactaata atcccttggg taatggtctg
    29821 cccgaggttg tacgggttac cgatcgccag tactacgtcg ccaatgtgcg gtacgcgacg
    29881 tgcattaatt ggaatggtag gtaaaccgcc agtggcatta attttaagta ccgccagatc
    29941 ggttagagag tcagatccca ccagcaatgc ttcaaataca cgtccatcct gtaaggcgac
    30001 gatgatctga tcggcgtcgt tgatgacgtg tttattggtg atgatataac cgcgttgatc
    30061 catgattaca ccggatccca gggtgcggat ctcaagctgg ttgtgagagt tggtgttcaa
    30121 accacggttg taaacgttaa ccaccgctgg cgcggcgcgg cgaaccgcca gattatagct
    30181 ggcaggcgtc tcatcggtac tgtcaaattg cggagtggaa agcgggttaa ggctgcgcag
    30241 cgaaggcatg gcaaccagca gaatagcgcc gacaattaat ccaatcgcaa cggaacgtaa
    30301 gagcttcaca aacatgatgg aggcgtcatt aaaaaaggga acggcagcag cataccacga
    30361 gttaaccgga catcacacgt cagcctgatg cccggtttac gacattaacg catcagcaga
    30421 tagatgcttt cattgccgcg tacaatttgc agggcgatga tggccggttt tgccgccagc
    30481 actttacgca tttcagcaat cgagttcacc cgatcgcggt tgacgccaat gatcacatcg
    30541 tctttttgca agccagcctg agcagctggg cttcctttga caacttcatc tatcttaata
    30601 cctttgccgc catcttttag ctgaccatcg ctcaacgttg caccttccag cgctggccga
    30661 cgaagaggtg ctggtatcaa gcgtcacttc tacttccagt ggtttgccgt tacgcagcag
    30721 gccaagcttc actttcgtgc ccggctctgt agtcgcgata cgagagcgca actcagcaaa
    30781 gctattcagc ggtttgccgt tgaggctggt aataatatcg cccgctttga cgcccgcttt
    30841 cgctgagcca gaacttggca acacttcgct gacaaacgcg ccacgctgca cgtcaaggtt
    30901 gaaggctttg gcgatatcgg cactcatctc ggtgcctttg atgcctaaca aaccgcgttt
    30961 gatttcacca aagtcgataa gctgctgcgc cagtgttcgc gccatattac tggggatggc
    31021 aaatccaatc ccgacgctcc cgccgccagg cgcaaggatt gcagtgttga tgccaattaa
    31081 ctcaccgtta aggtttaata gtgcaccgcc ggagttaccg cggttaatgg aagcatctgt
    31141 ctggataaag ttttccagac cttcaagatt caacccgctg cggcctaatg cggaaacaat
    31201 gccagaggtg gcggtttgcc caaggccaaa tgggttaccg accgctacgg caaaatcacc
    31261 gacgcgcaat ttatcggagt cggcaatagc gatttgcgtt aatttgctcg ggttttgaat
    31321 ttgtaacagg gcgatatcgc tctggtcatc gctaccaatc agttttgcat caaactcgcg
    31381 cccatcattg agctgaatac tgattttctg tgcctgatta atcacatggt tgttggtcag
    31441 cacatagcct ttactggcgt tgatgatgac accggaacct aaaccttcga agggttgtgc
    31501 aggttgatcc ggtaaatcat caccaaaaaa ctttttgaat tcttccggga ttttctgtcc
    31561 ctgactggcc gttccttcca cccgtacgct caccactgcc ggaagcactt tttccagcat
    31621 tggagccaga ctggggagag gggcctgatc ggcaacctgg cctggaatcg acgcgacggc
    31681 ctgaaatgac gccgagagag ttaacccggc acttaacgct aatgcactca acagctgggt
    31741 ttgttttttc attattcctg ctctcgtacc tgaatgataa gaaaagagat tcaaaacagt
    31801 ttgatgttat tgaattttca gacggctaac aatgagacac cagattaaat gatagtcggg
    31861 gagggagaga agaggagggc gcaatggctg cgcccgaaaa ataaattagt cgcgcttcgc
    31921 gccagtacgc agcaggccgg atgcgccttc agaatagtcg cgaggcacct gcaccggtgc
    31981 ctgatcgttg ctggcttcag actctgccag acgattacgg aacgggtttg cttcagcaga
    32041 cagttccggc agcaggctgc tggagctttt tgccatgtgc tgatacagct ggcgatagtc
    32101 gtgcgccatg gtatccagta attccgcgct gcgggcaaag tggctaacca gctcttcgcg
    32161 atactcgtcc agttcagctt tattcttttc cagttcgtac tgcaacgcct gttgctggcg
    32221 tagtttacga ttaccaaaac gcatggccac agcaccaata atgatgccga cgactaaccc
    32281 aattagcgca tattcccagg tcatgaacat ctcccgttgt cttttgtttc cgtagggtgt
    32341 tggcttcagg ctccatgcct gcggctgatt atgccactat aaccgttaat tccacagaag
    32401 tggaatcccg actgcatatc gcgtagtgta gaacggcctt tttttcgtca accgtgaaca
    32461 acggcgcacc gattattcaa ggaataacaa taagatcatg caaagcgtta ccccaacatc
    32521 gcaatacctg aaggcgctca atgaaggcag ccatcaaccc gacgacgttc aaaaagaggc
    32581 cgtcagccgc ctggaaatta tttatcaaga actcatcaat agcaggccac cagcccccag
    32641 gacgagtggg ctaatggcgc gggtcggtaa gctgtggggt aaacgcgaag acacaaagca
    32701 tacgccagtg cgtggcttat atatgtgggg cggtgtagga cgcgggaaaa cctggctgat
    32761 ggaccttttc tatcaaagcc tgccgggaga gcggaaacag cgcctgcact ttcaccgttt
    32821 tatgctgcgg gtgcacgaag agctaactgc cttacagggg cagaccgatc cgctggaaat
    32881 tattgccgat cgctttaaag ccgaaactga cgtgctctgt tttgacgaat tttttgtttc
    32941 tgatattacc gacgccatgc tacttggcgg tctgatgaaa gccctgttca cccgcggtat
    33001 taccctggta gcgacgtcaa atattccgcc ggacgaactt tatcgaaatg gcctgcaacg
    33061 tgcgcgtttt ctgcctgcaa tcgatgccat taaacagcat tgtgatgtaa tgaacgtgga
    33121 cgctggtgtt gattatcgac tgcgtacact cactcaggcg catctgtggc tttcgccact
    33181 caacgatgaa acccgggcgc agatggataa actatggttg gcgctggcgg gggtgaaacg
    33241 agaaaattca ccgacgttag aaatcaacca tcggccattg gcgacaatgg gcgtcgagaa
    33301 ccagacgctg gcggtttctt ttactacgct gtgcgtcgac gcccgcagtc agcatgacta
    33361 tattgcgctc tcacgcctgt ttcacacagt aatgttgttt gatgtgccag tcatgacgcg
    33421 gttgatggag agcgaagcgc ggcgctttat tgcgctggtg gatgagtttt acgagcgcca
    33481 tgtcaaatta gtggtgagtg cagaagtgcc gctgtatgaa atttatcagg gtgagcggct
    33541 gaagtttgag ttccagcgtt gcctgtcacg tctgcaagag atgcaaagcg aagagtatct
    33601 gaagcgcgag catttggcgg gttaaaacct gccacaaatc acaaaaaggg gtcgatcttt
    33661 gaccccgact tctctataat cctgcgaccc cacgttacaa gaaagttttt ttcccaaaac
    33721 tttttgtgtg ctggcatagg ctattcgaag gggtaggttt gccggacttt gtcgtgtgaa
    33781 cctcaacaat tgaagacgtt tgggtgttca ccaacgtgta actatttatt gggtaagctt
    33841 ttaatgaaaa cttttacagc taaaccagaa accgtaaaac gcgactggta tgttgttgac
    33901 gcgaccggta aaactctggg ccgtctggct actgaactgg ctcgtcgcct gcgcggtaag
    33961 cacaaagcgg aatatactcc gcacgtagat accggtgatt acatcatcgt tctgaacgct
    34021 gacaaagttg ctgtaaccgg caacaagcgt actgacaaag tgtactatca ccacaccggc
    34081 cacatcggtg gtatcaaaca agcgaccttt gaagagatga ttgctcgccg tcctgagcgt
    34141 gtgattgaaa tcgcggttaa aggcatgttg ccaaaaggcc cgctgggtcg tgctatgttc
    34201 cgtaaactga aagtttacgc gggtaacgag cacaaccacg cggcacagca accgcaagtt
    34261 cttgacatct aatcgggatt ataggcaatg gctgaaaatc aatactacgg cactggtcgc
    34321 cgcaaaagtt ccgcagctcg cgttttcatc aaaccgggca acggtaaaat cgtaatcaac
    34381 caacgttctc tggaacagta cttcggtcgt gaaactgccc gcatggtagt tcgtcagccg
    34441 ctggaactgg tcgacatggt tgagaaactg gacctgtaca tcaccgttaa aggtggtggt
    34501 atctctggtc aggctggtgc gatccgtcac ggtatcaccc gcgctctgat ggaatacgac
    34561 gagtccctgc gttctgaact gcgtaaagct ggcttcgtta ctcgtgacgc tcgtcaggtt
    34621 gaacgtaaga aagtcggtct gcgtaaagca cgtcgtcgtc cgcagttctc caaacgttaa
    34681 ttggcttctg ctacggcaga aaacaatttg cgaaaaaacc cgcttcggcg ggttttttta
    34741 tggctaaatt ctgaatcagc gtaaaaaatg ggaagttgct ttttgctgcc gtctgaaaga
    34801 caggtaaaac aaaaaccaca tcgccaataa gggtctaagt caactatttc agactaaagc
    34861 gcatctcttt ttccccattt ccggcatcca ctcaccacaa aggtcgcaaa atctggtaaa
    34921 ctatcatcca attttctgcc caaatgtcgg gtattgctca ttttttgttt gattttcgaa
    34981 caaagagagt cgttccttat tgggtaacac aacttctgac tggccacctg gtggctggta
    35041 gcagtaaaaa ttctgactat acctggaggt tttcatggct gtcgctgcca acaaacgttc
    35101 ggtaatgacg ctgttttccg gtcctactga catctatagc catcaggtcc gcattgtgct
    35161 ggctgagaaa ggtgtaagtt tcgagatcga acacgtggaa aaggacaatc cgcctcagga
    35221 tctgattgac ctcaacccga atcagagcgt tccgaccctg gtggatcgtg agctgaccct
    35281 gtgggaatct cgcatcatta tggaatatct ggatgagcgt ttcccgcatc cgccactgat
    35341 gcctgtttac ccggtagctc gcggtgaaag ccgtctgtac atgcatcgca tcgaaaaaga
    35401 ctggtacacg ctgatgaaca ctatcatcaa cggttcagct tctgaagcag atgccgcacg
    35461 taagcaactg cgcgaagaac tgctggcgat tgcgccggtc ttcggtcaga agccgtactt
    35521 cctgagcgat gagttcagcc tggtcgattg ctaccttgct ccgctgctgt ggcgtctgcc
    35581 gcaactgggc atcgagttca gcggcccggg tgcgaaagag ctgaaaggct atatgacccg
    35641 cgtctttgag cgtgactctt tccttgcttc tttaactgaa gcagaacgtg aaatgcgtct
    35701 gggccggagt taatctgtat ggatttgtca cagctaacac cacgtcgtcc ctatctgctg
    35761 cgtgcattct atgagtggtt gctggataac cagctcacgc cgcacctggt ggtggatgtg
    35821 acgctccctg gcgtgcaggt tcctatggaa tatgcgcgtg acgggcaaat cgtactcaac
    35881 attgcgccgc gtgctgtcgg caatctggaa ctggcgaatg atgaggtgcg ctttaacgcg
    35941 cgctttggtg gcattccgcg tcaggtttct gtgccgctgg ctgccgtgct ggctatctac
    36001 gcccgtgaaa atggcgcagg cacgatgttt gagcctgaag ctgcctacga tgaagatacc
    36061 agcatcatga atgatgaaga ggcatcggca gacaacgaaa ccgttatgtc ggttattgat
    36121 ggcgacaagc cagatcacga tgatgacact catcctgacg atgaacctcc gcagccacca
    36181 cgcggtggtc gaccggcatt acgcgttgtg aagtaataca aaacaggccc aggcggcctg
    36241 ttttgtcttt ttaatgactt aataaaatag cgtgattgtg gcaatcatat tcaccacgaa
    36301 accgactgcc atggggatcg ctgtccgctt caccaccgca aaaggcgaaa cgcctgcaat
    36361 tcctgacgta acgaccacca ccgcagtaat cggcgaaacc gcgcgcgcta gcgtcgtggc
    36421 aaaatgcatc ggcataatca ttacaaccgc tggtacatgt agtccggctg cgatattcgg
    36481 aataagactg gcaaatgaca taaacggcgc attgccagag cccatcacaa tggcacaaat
    36541 ggcaatgact agcgccataa taatcatcac gccaataccg cccagaccag aatgctccgc
    36601 acccctgata accgcatcaa cagtgccaat cgtcgttaag cctttcgcaa atatttcacc
    36661 cgcgaccacc agcgttacca cgttgacaaa ctgcgtaccc atgccgtcaa aaaacgcctg
    36721 cacatcgtcc attgtttcgc gcaagttatg cttgcggaag aactcaacaa acatcgtcac
    36781 cgtcaaactt agtaacatca ccaccaccag atgcagttct gactgcatca gcccgacgtg
    36841 ggcgaggaac agcgagccga gcatcaggat taacggcatc acaggtaaaa tggcgtaata
    36901 gagcggcggg acattatcga gagttttttg ctctgcctgt tcgtgattga tatttttatc
    36961 ttttttgtca aaagcgcgtt gcacgaaaaa gtgggagatc gccaccgaga taatgacgca
    37021 aga
//
LOCUS       DAJTSF010000030        36587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000030 DAJTSF010000000
VERSION     DAJTSF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.030"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(156..1391)
                     /locus_tag="OO335_001609"
     CDS             complement(156..1391)
                     /locus_tag="OO335_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6002042.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="OO335_001610"
     tRNA            complement(1538..1614)
                     /locus_tag="OO335_001610"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="OO335_001611"
     tRNA            complement(1657..1743)
                     /locus_tag="OO335_001611"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="OO335_001612"
     tRNA            complement(1764..1839)
                     /locus_tag="OO335_001612"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="OO335_001613"
     tRNA            complement(1898..1974)
                     /locus_tag="OO335_001613"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="OO335_001614"
     CDS             complement(2077..3462)
                     /locus_tag="OO335_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6002043.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OO335_001615"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OO335_001615"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6002044.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OO335_001616"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OO335_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6002045.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OO335_001617"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OO335_001617"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6002046.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OO335_001618"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OO335_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6002047.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OO335_001619"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OO335_001619"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6002048.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OO335_001620"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OO335_001620"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6002049.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OO335_001621"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OO335_001621"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6002050.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OO335_001622"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OO335_001622"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6002051.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OO335_001623"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OO335_001623"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6002052.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OO335_001624"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OO335_001624"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6002053.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OO335_001625"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OO335_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6002054.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OO335_001626"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OO335_001626"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6002055.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OO335_001627"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OO335_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6002056.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OO335_001628"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OO335_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6002057.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OO335_001629"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OO335_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6002058.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="OO335_001630"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="OO335_001630"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6002059.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="OO335_001631"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="OO335_001631"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6002060.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OO335_001632"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OO335_001632"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6002061.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="OO335_001633"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="OO335_001633"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6002062.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OO335_001634"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OO335_001634"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6002063.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="OO335_001635"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="OO335_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6002064.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OO335_001636"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OO335_001636"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6002065.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OO335_001637"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OO335_001637"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6002066.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OO335_001638"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OO335_001638"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6002067.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OO335_001639"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OO335_001639"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6002068.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="OO335_001640"
     CDS             complement(31267..32913)
                     /locus_tag="OO335_001640"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6002069.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="OO335_001641"
     CDS             complement(32916..32966)
                     /locus_tag="OO335_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002070.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OO335_001642"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OO335_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6002071.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..34981
                     /locus_tag="OO335_001643"
                     /pseudo
     CDS             33502..34981
                     /locus_tag="OO335_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="OO335_001644"
     CDS             complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="OO335_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6002072.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35463..36302
                     /gene="hdfR"
                     /locus_tag="OO335_001645"
     CDS             35463..36302
                     /gene="hdfR"
                     /locus_tag="OO335_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6002073.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36398..36473)
                     /locus_tag="OO335_001646"
     tRNA            complement(36398..36473)
                     /locus_tag="OO335_001646"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36438..36440),aa:Trp,seq:cca)
     gene            complement(36482..36558)
                     /locus_tag="OO335_001647"
     tRNA            complement(36482..36558)
                     /locus_tag="OO335_001647"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36522..36524),aa:Asp,seq:gtc)
BASE COUNT         8940 a        10012 c         9217 g         8418 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acggacgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcactgg agatcccatt
    34621 accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa
    34681 acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg
    34741 gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg cgatgtggtt
    34801 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tactgaaagt
    34861 tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga
    34921 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
    34981 aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct tcagcacctt
    35041 caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta atacgcgtca
    35101 tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca cggcatactg
    35161 ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga
    35221 aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca ccatgacgag
    35281 agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg
    35341 ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg
    35401 cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca
    35461 ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc
    35521 gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc agacaactgg
    35581 aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt ttaaccgctg
    35641 ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgca
    35701 aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc gcctcgttgt
    35761 gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat gcccatacag
    35821 gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ctgcatgaac
    35881 gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt agtagtcagt
    35941 tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta aagggagatc
    36001 ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca ggtttgatcg
    36061 gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag caacagattg
    36121 cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc
    36181 ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg ctgcaaaata
    36241 gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat gaagtgtatt
    36301 aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg attatttcag
    36361 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
    36421 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
    36481 gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac
    36541 cgactgaact accgctccac cgaattcttt tacaaccacc ggtttta
//
LOCUS       DAJTSF010000031        36222 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000031 DAJTSF010000000
VERSION     DAJTSF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.031"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..421
                     /locus_tag="OO335_001648"
     CDS             <1..421
                     /locus_tag="OO335_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6002074.1"
                     /translation="DVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDR
                     GSQYCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAI
                     SVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            463..711
                     /locus_tag="OO335_001649"
                     /pseudo
     CDS             463..711
                     /locus_tag="OO335_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(758..1516)
                     /gene="deoR"
                     /locus_tag="OO335_001650"
     CDS             complement(758..1516)
                     /gene="deoR"
                     /locus_tag="OO335_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6002075.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1574..2170)
                     /gene="ybjG"
                     /locus_tag="OO335_001651"
     CDS             complement(1574..2170)
                     /gene="ybjG"
                     /locus_tag="OO335_001651"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6002076.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2455..3681
                     /gene="mdfA"
                     /locus_tag="OO335_001652"
     CDS             2455..3681
                     /gene="mdfA"
                     /locus_tag="OO335_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR6002077.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3722..4006)
                     /locus_tag="OO335_001653"
     CDS             complement(3722..4006)
                     /locus_tag="OO335_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002078.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(4092..4907)
                     /gene="ybjI"
                     /locus_tag="OO335_001654"
     CDS             complement(4092..4907)
                     /gene="ybjI"
                     /locus_tag="OO335_001654"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6002079.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4907..6115)
                     /locus_tag="OO335_001655"
     CDS             complement(4907..6115)
                     /locus_tag="OO335_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6002080.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            6199..6735
                     /gene="rcdA"
                     /locus_tag="OO335_001656"
     CDS             6199..6735
                     /gene="rcdA"
                     /locus_tag="OO335_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6002081.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6910..8595)
                     /locus_tag="OO335_001657"
     CDS             complement(6910..8595)
                     /locus_tag="OO335_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6002082.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8865..9242
                     /locus_tag="OO335_001658"
     CDS             8865..9242
                     /locus_tag="OO335_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6002083.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(9272..9529)
                     /gene="grxA"
                     /locus_tag="OO335_001659"
     CDS             complement(9272..9529)
                     /gene="grxA"
                     /locus_tag="OO335_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6002084.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9689..9976
                     /locus_tag="OO335_001660"
     CDS             9689..9976
                     /locus_tag="OO335_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6002085.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9960..10682
                     /gene="nfsA"
                     /locus_tag="OO335_001661"
     CDS             9960..10682
                     /gene="nfsA"
                     /locus_tag="OO335_001661"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR6002086.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10743..11645
                     /gene="rimK"
                     /locus_tag="OO335_001662"
     CDS             10743..11645
                     /gene="rimK"
                     /locus_tag="OO335_001662"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6002087.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11733..12209
                     /locus_tag="OO335_001663"
     CDS             11733..12209
                     /locus_tag="OO335_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6002088.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12561..13673
                     /gene="potF"
                     /locus_tag="OO335_001664"
     CDS             12561..13673
                     /gene="potF"
                     /locus_tag="OO335_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6002089.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13768..14901
                     /gene="potG"
                     /locus_tag="OO335_001665"
     CDS             13768..14901
                     /gene="potG"
                     /locus_tag="OO335_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6002090.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14911..15864
                     /gene="potH"
                     /locus_tag="OO335_001666"
     CDS             14911..15864
                     /gene="potH"
                     /locus_tag="OO335_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6002091.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15861..16706
                     /gene="potI"
                     /locus_tag="OO335_001667"
     CDS             15861..16706
                     /gene="potI"
                     /locus_tag="OO335_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6002092.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16766..17254
                     /locus_tag="OO335_001668"
     CDS             16766..17254
                     /locus_tag="OO335_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6002093.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            17295..18422
                     /gene="rlmC"
                     /locus_tag="OO335_001669"
     CDS             17295..18422
                     /gene="rlmC"
                     /locus_tag="OO335_001669"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6002094.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18597..19328)
                     /gene="artJ"
                     /locus_tag="OO335_001670"
     CDS             complement(18597..19328)
                     /gene="artJ"
                     /locus_tag="OO335_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6002095.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19620..20288)
                     /gene="artM"
                     /locus_tag="OO335_001671"
     CDS             complement(19620..20288)
                     /gene="artM"
                     /locus_tag="OO335_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6002096.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(20288..21004)
                     /gene="artQ"
                     /locus_tag="OO335_001672"
     CDS             complement(20288..21004)
                     /gene="artQ"
                     /locus_tag="OO335_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6002097.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(21011..21742)
                     /gene="artJ"
                     /locus_tag="OO335_001673"
     CDS             complement(21011..21742)
                     /gene="artJ"
                     /locus_tag="OO335_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6002098.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21760..22488)
                     /gene="artP"
                     /locus_tag="OO335_001674"
     CDS             complement(21760..22488)
                     /gene="artP"
                     /locus_tag="OO335_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6002099.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22706..23221)
                     /locus_tag="OO335_001675"
     CDS             complement(22706..23221)
                     /locus_tag="OO335_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6002100.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23870..25639
                     /locus_tag="OO335_001676"
     CDS             23870..25639
                     /locus_tag="OO335_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6002101.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25850..26173
                     /locus_tag="OO335_001677"
     CDS             25850..26173
                     /locus_tag="OO335_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6002102.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            26170..27000
                     /gene="amiD"
                     /locus_tag="OO335_001678"
     CDS             26170..27000
                     /gene="amiD"
                     /locus_tag="OO335_001678"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6002103.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26997..28010)
                     /locus_tag="OO335_001679"
     CDS             complement(26997..28010)
                     /locus_tag="OO335_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6002104.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(28109..29539)
                     /locus_tag="OO335_001680"
     CDS             complement(28109..29539)
                     /locus_tag="OO335_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6002105.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29550..30551)
                     /gene="ltaE"
                     /locus_tag="OO335_001681"
     CDS             complement(29550..30551)
                     /gene="ltaE"
                     /locus_tag="OO335_001681"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6002106.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30588..32306)
                     /gene="poxB"
                     /locus_tag="OO335_001682"
     CDS             complement(30588..32306)
                     /gene="poxB"
                     /locus_tag="OO335_001682"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6002107.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(32439..33407)
                     /gene="hcr"
                     /locus_tag="OO335_001683"
     CDS             complement(32439..33407)
                     /gene="hcr"
                     /locus_tag="OO335_001683"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6002108.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(33419..35071)
                     /gene="hcp"
                     /locus_tag="OO335_001684"
                     /gene_synonym="priS"
     CDS             complement(33419..35071)
                     /gene="hcp"
                     /locus_tag="OO335_001684"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6002109.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(35215..36114)
                     /gene="lysO"
                     /locus_tag="OO335_001685"
     CDS             complement(35215..36114)
                     /gene="lysO"
                     /locus_tag="OO335_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6002110.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         9044 a         8954 c         9305 g         8919 t
ORIGIN      
        1 agatgtttat acgtgcgaaa ttgtcggcta cgccatggga gagcgcatga caaaagagct
       61 gacaggtaaa gccctgttta tggcgctcag gagccagcgc ccacctgccg ggctaatcca
      121 ccactctgat cgaggttcac agtactgcgc atacgattac cgggtcatac aggagcagtc
      181 tggtctgaaa acatcaatgt cgcgtaaagg taactgttac gacaacgctc cgatggaaag
      241 cttctgggga acgctgaaaa atgagagcct gagccactat cgttttaata accgggatga
      301 agccatctca gtaatacggg aatacattga gattttctac aatcgtcagc gtcgtcactc
      361 tcgtctgggg aatatctccc cggcagcctt cagggaaaaa tatcatcaga tggctgctta
      421 aaaaagaaca aatggtagtg tccgctattg ccagtacacc tcaccgtgac cataccgcgt
      481 gggcagctga aaaacctgaa ggcgagctac acgttaacgg aaccgcagct taccgcaccg
      541 ctgaaaaaag gtcaggttgt cgggaccatt gatttccagc ttaacggtaa atccattgag
      601 cagcgtccgc tgatcgtgat ggaaaatgtg gaagagggcg gattctttgg taggatgtgg
      661 gatttcgtga tgatgaaatt ccatcagtgg ttcggcagct ggttctctta atcttctgat
      721 aaccggatgg cgcgaaacgt catccggtta tacgtcatta atacatcaac ttaatacggt
      781 gcgtctgcgc gtacttcaca tactcatctt ccgggcaaca atcactcacc acaatatcaa
      841 agcgtttcag gtcacccatg cgcgccggac gcaccttgcc aaatttactg tggtcgacaa
      901 ccagcacatg cttttgcgcc atcgacatgg cccagtgttt taccggcaac tcttcaagat
      961 taaaacaggt agcgccttta ctgacatgca cgcccgccgc ggaataaaaa gcgatatccg
     1021 ggcaaaaatt attcaacgtt tgctgaaaat cgatgggttt gaaaatggcg ttgctggcgt
     1081 gaaattcacc accgcacaga aatgcgcggc aatggggttt ctctttcagc gccagaaagg
     1141 tatttagcga ataacaaacg gcggtaaaag ggatttcatt atcaatcgct tcaataatcc
     1201 acggcgtggt ggtgccacag tcaaaaaaga gggtctgatc gggttctacc agtgtcgcag
     1261 ccagttttgc cgcccggcgt ttttcttcca ccaggcggga tttttgatcg cttaacaggt
     1321 aatggctggc actgcgcggt tccagaacaa tatagccgcc gagcaaaacg acgggcgcac
     1381 tgtggttgtt cagatcgcga cgaatcgtca tctccgaaac cccaagcagg gcggcggcgt
     1441 ctttaagatg taacttatcg ctgcgtttta attcttgcag cagctgcccg atacgctctt
     1501 cgcgacgtgt ttccataatc cctctgaata gttattgaag cgagccgctc aatactacac
     1561 tttttagcag agatcagtca cgcacccagc ctttgcggat cggtaatgca aaacagacgc
     1621 gataccacga ttgcagacgt tgatagagtt tatgccccat cgcttgccag ataatctggg
     1681 cactaaggca gccaatcata cctgccagca atccaccgag catatccagc ggccagtgga
     1741 cgccaagata aacgcgcgac caggcaatga cgacggccag caccattaaa agtgagccgg
     1801 accacaggcg atgccagcat aaaaatgcca gtgcaaaggt gaaaatcacc gtaccgtgat
     1861 cgctcgggaa tgagtcatcc gccgcatgat gcaggaagtt atagccgata ttttcgacaa
     1921 agggtcggtc gtgcggaaaa agatgtccca tcgtccagga cacaaacagg ctgaccgcca
     1981 gcgcgatagc gatttttatc accagttgcc gttgtgctgt aagcccccac aaccaaagta
     2041 ccgcggccag caacggcacc acggtaatca aatctttagc aataaaaatc gccaacgaga
     2101 tcatccacgg agccgagtct ggcgtcgcgt taataagaga gaatagagag agattcaaat
     2161 tttccagcat aacttcccga cgcaaagtga ttaaaaggga gccaatacag gcaagtcgtt
     2221 gagaataaag tgcaggttaa actgggtaaa gcggcatcgt cttatttccc tcaagcggcc
     2281 tgtttacggt gggtgattgt aacgggcata ggttaaataa aacttaaaga aagcgtagct
     2341 atactcgcaa taatgtaaga atgtgcttaa ccgtggtttc agctacaaaa ttcgctttct
     2401 cgttagctgc gcttttatta aactctgcgc gattattatt ggcgaagaaa ttgcatgcaa
     2461 aataaattag cttccggtgc caggcttgga cgtcaggcgt tacttttccc tctctgtctg
     2521 gtgctttacg aattttcaac ctatatcggc aacgatatga tccaacccgg tatgttggcc
     2581 gtggtggaac aatatcaggc gggcattgat tgggttccta cttcgatgac cgcgtatctg
     2641 gcgggcggga tgtttttaca atggctcctg gggccgctgt cggatcgtat tggtcgccgt
     2701 ccggtgatgc tggcgggtgt ggtgtggttt atcgtcacct gtctggcaat attactggcg
     2761 caaaacattg aacaattcac cctgttgcgc ttcttgcagg gcataagctt ctgtttcatt
     2821 ggcgctgtgg gatacgccgc aattcaggaa tccttcgaag aggcggtttg tatcaagatc
     2881 accgcgctga tggcgaacgt ggcgctgatt gctccgctac ttggtccgct ggtgggcgcg
     2941 gcgtggatcc atgtgctgcc ttgggagggg atgtttgttt tgtttgccgc attggcagcg
     3001 atctcctttt tcggtctgca acgagctatg cctgaaaccg ccatgcgtat aggcgagaaa
     3061 ctgtcactga aagaactcgg tcgtgactat aagctggtgc tgaagaacgg ccgctttgtg
     3121 gcgggggcgc tgggattcgt tagcctgcca ttgctggcgt ggatcgccca gtcgccgatt
     3181 atcatcatta ccggggagca gttgagcagc tatgaatatg gcttgctgca agtgcctatt
     3241 ttcggggcgt taattgcggg taacttgctg ttagcgcgtc tgacctcgcg ccgcaccgta
     3301 cgttcgctga ttattatggg cggctggccg attatgattg gtctgttggt cgctgctgcg
     3361 gcaacggtta tctcatcgca cgcgtattta tggatgaccg ccgggttaag tatttatgct
     3421 ttcggtattg gtctggcgaa tgcgggactg gtgcgattaa ccctgtttgc cagcgatatg
     3481 agtaaaggta cggtttctgc ggcgatggga atgctgcaaa tgctgatctt taccgtcggt
     3541 attgaaatca gcaaacatgc ctggctgaac gggggcaacg gactgtttaa tctcttcaac
     3601 cttgtcaacg gaattttgtg gctgtcgctg atggttatct ttttaaaaga taaacagatg
     3661 ggaaattctc acgaagggta aaaaaatgcc tgattgattt gtgcgatcag gcattctcga
     3721 attaatggtg atggtcgtca atctggtgtt cgataaccat cccttcacct acgctggcaa
     3781 gatggcgaac ataaggatgc gggcggtaag ccggagctgg cgcaggagcc acatagacgg
     3841 tttgcggcac agtcgcgacg ctgaccgctt gtggaacgct gactgaatca ggaaccacaa
     3901 ccactttgta tccactcggc acatcgacgg tgacactttg cgccatcgcc acaccagtaa
     3961 agcccattaa aagcgcgccc aacagtagac aatttttcat aatcatttcg cctgaaataa
     4021 tgcagcgggt taatttccgc gaattatgca gccttcattc aggcattgtg ttgccgggcg
     4081 tgctgccgtt tttattggtc aaatggcgct tcgtgtttaa gaactttatc gatcacatcc
     4141 agtacgcctt cacggttatt gtagcctgcc cggtattttg ccgctgcgac gactgcgctg
     4201 ccggcatttt ccattgcaaa actaaagcct gcctgacgca gcatctcaat atcgttaccg
     4261 ccatcgccaa agaccaccac ttcgctgtcg tctattcccc ataatttctg cagttggcga
     4321 aggccattgg ctttatgtac gccggggata atcagatcga tgctgccgtt gccggtgtgg
     4381 actggcacca taatatcgcc gatggcctca tgtaatgctt tttgtacttg tggaatcagt
     4441 tcatcggaaa gattcaggcc aaacttaaag aaaatatctt ctaagttgtc aaagttatcg
     4501 acgtattcca gacggtgata atacatttcc gccaccgttt tcatggcatc gtcatatttt
     4561 ttgagtgtat aggcgctatt ttttccgcag gcaataattt ccacttccgg gcgcgtcagc
     4621 aaatgttcca cgacagtagc aaacgcatcc ttcgacagct cgccattaaa aacatctttg
     4681 ccttcactca ttacccagcc gccgttttca gccacaaagg caatttcatt cgcaatttca
     4741 gggaagaaag agatcaactg ataatattga ttcccgctgg cgaccacaaa gcgaatgcct
     4801 tgtgctttca tttgctgata ctgagccata aaccgctcac ggttataggt tttttgatcg
     4861 cttaagaaag taccatccat gtctaccgca attaatttaa tgctcatcaa ctattctcca
     4921 tcgccgtctg cgttttggta tcgggtttgg cgacggcttt cgcgacaata gccgcgagaa
     4981 taaccagcgc cagtacaacc agcattgcac tacgtaatcc ataatgttcg ccgagatagc
     5041 ccagcagcgg cggcccgacg aggaaagcca gataaccggt cgttgctacc acactgacgc
     5101 gggttggtgc atcggggccg gtatcgctgg cggcagaaat ggtcagcggg aagcccagcg
     5161 aggcacccag tccccaaagt acaacagaca ctcctgcgac ccaggcgcta tcgacaaaaa
     5221 taatcagccc aatacccaac gcccccatta gtgcactggc ccgaaccacg gcaacgcgac
     5281 tgtaactgtc gatgaaccaa ccgccggtga agcgtccaac ggtcatcccc agggtaaaac
     5341 cggcataaat cagtgagccg gaagtggggc taaaaccgtg accatcaacc attaataagg
     5401 gtaaccagtc gttggcagaa ccttcggcaa aggccatcgc cagcactaca acaccaatca
     5461 acagcaactg gatatcgcga taaaaaggaa tgcctttttc gccatgctgg gtgccatcgg
     5521 cagcattttt gcccgtaccg tcagggattg cctgaatggc gatataaata ggcgcgatac
     5581 ctaccagcgc cgccaataaa atgtgcaccg ttgccggaac gccaaaggcc gtcagtgcca
     5641 tcccgacacc agcgcctgcc agcgtgccca ggctataaaa accgtgcatc atcggcaaaa
     5701 ccgttttatt catttctcgc tcaacggcgg caccttcaac gtttatcgcc acttccgcag
     5761 aaccaaaact tgccccaaag acgccgagac caacggcaaa gagcaggggc gatgtcagcc
     5821 agagtgccag acttaatatc atcatcccga tcaatgcgca ggacatcgtg accaggatga
     5881 cattacgtgt cccaaagcgt ttcactaacc acgccgagca gagaataccg ctcatcgaac
     5941 cgatcgacag accaaagaga acaccgccca tttcagcgat cgagacagag agaatatcgc
     6001 ggatagcagg cgtacgggtt gcccaggacg ccattaacag gcctggcaaa aagaagaaca
     6061 taaacagcgc ccaggttcgg cgtttcaatg cattacgtga agaatttacg gtcatagatc
     6121 acgtcaaaat aagaagaggg aagacaacat tagcaaggtt gtgtacattt gtacacaatt
     6181 gcagacagag gaaatgacat gcgtcgcgct aacgatccgc aacggcgaga aaaaattatc
     6241 caggccacgc tggaggcggt gaaactttac ggaatacatg ctgttacgca ccgcaaaatt
     6301 gctacccttg ccggggtacc gttgggatcg atgacctact atttttcagg aattgatgag
     6361 ttgttgctgg aggcgttcag cagttttact gagattatgt cccggcaata tcaggcattt
     6421 tttagcgatg ttagtgatgc tcaaggcgca tgccaggcta tcaccgatat gatctacagc
     6481 tcacaggttg caacgccgga taatatggag ctgatgtacc agctctacgc gctggctagc
     6541 cgaaaaccgc tattaaaaac ggtaatgcaa aactggatgc agcgcagtca gcaaacgctc
     6601 gaacaatggt ttgaacccgg aaccgcccgc gcgcttgatg cgtttattga ggggatgacg
     6661 ctgcattttg tcaccgaccg taagccgcta tcgcgcgagg agattttgag gatggttgag
     6721 agggttgcag ggtagtagat aaattttaga taacaaaaaa cccatcaacc ttgaaccgaa
     6781 atggcggggt tgatgggctc cacaaaatgg ggacatcaaa gaaaagcagt ggcactaatt
     6841 aagactgatg ccctgcggaa aagttctgcg gttgtgcaaa aaaatttcat tttcagggca
     6901 acttcagttt tatcctaatc ctggccatac catgacgatg attgtccctg ccagcgtcag
     6961 caggacgttg gcgattgcgt aggtgcccgc atagcccagc gcagggatgt tactgcgagc
     7021 tgtatcactg atgatctcca ttgccggcgc gcaggtgcgt gcgcccatca ttgcgccgaa
     7081 caacagtgcg cggttcattc gcaatacata agcaccgaac aagaaacaga taaccacggg
     7141 cacaagactg acaattaatc cggcaatcaa catctgaccg ccaatcgcgc ccaggccgtt
     7201 attaataccg ctaccggcgc tcagaccaac gcctgccata aacaccatca agccgaactc
     7261 tttcaccatg cttaatgcac cttgcggaat gtaaccgaag gtcgggtggt tagcacgcat
     7321 aaagcccagc ataattccgg cgaataacaa cccggcagcg ttccccatgc cgaaactgaa
     7381 tgtgctgaac tggaaggtga tcatcccgat catcagccca ataacaaaga aggcgcaaaa
     7441 tgccagcagg tcagtgacct ggctgtgaat cgagataaag ccgatgcgat cggcgatggt
     7501 ttttacgcga cgggcatcac cgctgacttg taaaacgtca cctttgttaa gcacgacgtt
     7561 gtcatctatc ggcatctcaa tctggctacg aatgacgcgg ttaaggaagc aaccgtgatc
     7621 ggtcaacttc agttgtgcga gacgtttacc tacagcgtta tggtttttaa cgaccacttc
     7681 ttcagtgacg atacgcatgt cgagaaggtc acgatcgaaa acttctttac cgttacggaa
     7741 gctgggatcg agtcgggcat gggcgtcggg atagcctacc aacgctattt catcgcccat
     7801 ttgtagcacg gcatcaccgt ctggatttgc cagaatcccg ttacgtcgaa tacgttcaat
     7861 gtagcagccg gtttgtcgat aaatacccag ttcacgcaga tttttgccgt cggtccaggc
     7921 caccagctcc gggccgacgc gataggcgcg gatcaccggt aaataaacct tacggttggc
     7981 atcagtgtcc aggccacgtt cgcgggcgat ttgctgggcg ctggtctgta agtcctgatg
     8041 ctgcaatttc ggcaagtaac gcgcaccaac aatcaaactc accagaccga ttaaataggt
     8101 taaggcatac ccgaggctca gattatccag tgccagtgag agctgcctgc tttccatgcc
     8161 ggaatgatgc agtgtatcgc cagcaccgac cagaaccggt gtcgacgtca tagagcctgc
     8221 taacataccg gccgtcaggc caatatccca gccaaacagc ttacctaacc ctaaggcgat
     8281 caccagcgca ctgccaacca tcaccagtgc taacattagg taatttttcc catcgcgaaa
     8341 aaaaatggaa aaaaagttcg gtccggcttc gaccccgacg cagaaaataa acagcataaa
     8401 gccaagatta agcgcatcgg tgttaatgct gaaatgttgt tggcctaata acagcgatac
     8461 gactaaaacg ccaatggaat tacccagttg gatcgaacca agtcgtaact ttccgagaca
     8521 tagcccaagc gcgaggacca caaataataa cagaatgtaa ttcccattta acaattcggc
     8581 gacgtttata ttcacggagg ctaacttctt gtttactagt aagctgttga aagaaatgct
     8641 aatttacgat aatgtgtttt accagaattc agggcgcaga ttcattcagc gcacctaaac
     8701 gatagtaaag taacaatata ttttactagt gtaatcacat taggtatcaa cggctatatg
     8761 aattgcgttg gcctatatta gcatggaatg cgaagcggct ttatcttact gaacgccaca
     8821 ctggcgaaaa atgtgttcga tagacgcagt gtcaggagga acgagtgaaa cataaacaac
     8881 gttgggcggg ggcaatctgc tgttttgtcc tcttcattgt ggtgtgcctt tttctggcga
     8941 cgcacatgaa aggcgctttt cgggctgccg ggcatcctga aatcggcttg ctgtttttca
     9001 ttcttcctgg agcagttgcc agtttctttt cacagcgtag agaagtcctg aaacctctat
     9061 ttggcgcaat gctggcggca ccctgttcaa tgctcattat gcggctgttt ttttcaccga
     9121 cgcgctcatt ctggcaagag ctggcatggt tactaagcgc ggtgttctgg tgtgcgctgg
     9181 gggcactgtg tttcttattt atcagtagtt tgtttaaacc acagcacaga aaaaatcagt
     9241 aaagccctca acgcgagggc ttgtcagacg atcaggcgtc cagattttct ttcacccatg
     9301 cagcaaaatc ggtatagccg ccgatatgtt gctgatcgac aaaaatctgc ggcacggttt
     9361 ctacgggttt acctgccttt tgttgtagat cttctttagt gatcccttcc gcacgaatat
     9421 ctacatactg atactgaaaa tcatcgcgtt cattgctcaa tttctcagcc agatcttttg
     9481 cacgcacaca gtaagggcaa cccgaacgac caaaaataac ggtttgcatt atttctctcc
     9541 tcatagattt atgcctgtaa tgatcacgct aaaatgtatt cgctgaaagt aggtttaacc
     9601 tgttgcatta attgctaaaa gctataactg ttaaacacaa tacagtgaaa agttttagac
     9661 tgaaggctca ctttgcagag ggaagcgtat gcgcgcgatc ggtaaattgc ctaaaggcgt
     9721 gttgatactg gaatttatcg gaatgatgct actggcggtg gcgctgctgt cggtaagcga
     9781 ctccctgtcg ctgcctgagc cattttctcg gccagaagtg cagattctga tgatttttct
     9841 cggtgttttg ctcatgcttc ccgctgcggt ggtggttatt cttcaggtgg caaaacgtct
     9901 tgccccacag ctgatgaact gtccaccgca atattcacgt tcagaaagag aaaaagataa
     9961 tgacgccaac cattgaactt atttgtggcc atcgcgccat tcgccatttc actgatgaac
    10021 ccatttccga agcgcagtgt gaggcgatta ttaacagcgc ccgtgcgacg tccagttcca
    10081 gttttttgca gtgtagtagc attattcgca ttaccgacaa agcattacgt gaagaactgg
    10141 tgacgctgac cggcgggcaa aaacacgtag cgcaagcggc ggagttctgg gtgttctgtg
    10201 ccgactttaa ccgccattta cagatctgtc cggatgctca gctcggcctg gcggaacaac
    10261 tgttgctcgg tgtcgttgat acggcaatga tggcgcagaa tgcattaacc gcagcggaat
    10321 cgctgggatt gggcggggta tatatcggcg gcctgcgcaa taatattgaa gcggtgacga
    10381 aactgctgaa attaccgcag catgttctgc cgctgtttgg gctgtgcctt ggctggcctg
    10441 cggataatcc ggatctcaag ccgcgtttac cggcctccat tttggtgcat gaaaacagct
    10501 atcaaccgct ggataaagac gcactggcgc agtatgacga gcaactggcg gaatattacc
    10561 tcacccgtgg cagcaataat cgctgggata cctggagcga tcatatccgc cgaacaatca
    10621 ttaaagaaag ccgcccattt attctggatt atttgcacaa acagggttgg gcgacgcgct
    10681 aaaaccgcca cgtcgatgta tgatacgcgg gcttttgacc aggtctgaca gagaggtgca
    10741 gggtgaaaat tgccatattg tcccgggatg gaacgctcta ttcgtgtaag cggctgcgtg
    10801 aagccgctat acagcgcggt cacctggttg aaattcttga tccgctttct tgctacatga
    10861 acataaatcc tgcggcgtct tctatttact acaaaggccg caagttaccc cattttgacg
    10921 cagtgatccc gcgtattggc accgccatta ccttttatgg gacggcggca ctgcgccagt
    10981 tcgagatgct ggggagctat ccgctcaatg agtcggtcgc cattgcccgg gcgcgtgaca
    11041 aattgcgttc catgcaactg ctggcgcgtc agggcatcga cctgcctgtc acgggcattg
    11101 cgcattcgcc ggatgatacc agcgatttaa tcgacatggt cggtggtgcg ccgctggtgg
    11161 tcaagttggt tgaaggcacg cagggaattg gcgtcgtgct ggcggagact cgtcaggcgg
    11221 cggaaagcgt gattgacgct ttccgcggtc tgaacgcgca tattctggtg caggaatata
    11281 tcaaagaggc gcaagggtgc gatatccgct gtctggttgt tggtgatgaa gtggtcgctg
    11341 cgattgaacg gcgggcgaaa gagggctatt ttcgttccaa tttgcatcgt ggcggcgcgg
    11401 caagcgtcgc cagtatcaca ccacaggagc gtgaaatcgc gataaaagcc gcgcgaacga
    11461 tggcgctgga cgttgctggt gtggatattc tgcgtgctaa tcgcgggccg ttggtgatgg
    11521 aagtgaatgc gtcgccgggg ctggaaggaa tagaaaaaac caccggtatc gatatcgcgg
    11581 gtaaaatgat ccgctggatc gaacgccacg ctacgacaga atattgcctg aaaacgggtg
    11641 gttagtcgca atcacattac tgatcatggt tttgcctgcg ctttttgcgt aagctgtgcc
    11701 ggtcttttta tcgaaagagg ttgtacaaaa ttatgacatc gctggtcgtt cctggtctgg
    11761 atacgctgcg tcaatggctc gatgacctgg ggatgagttt ttttgaatgt gataactgtc
    11821 aggctctgca tctgccccat atgcagaatt tcgacggtgt ctttgatgcc aaaatcgatc
    11881 tgatcgataa cacgatcctg ttttctgcca tggcggaagt ccgaccttca gccgtattgc
    11941 cgctggcggc ggatttatct gccatcaatg ccagttcgct gaccgtgaaa gcatttcttg
    12001 atatgcagga tgataatctg ccaaagctgg tggtttgcca gtctttatcc gttatgcagg
    12061 gcgtaaccta tgagcagttt gcatggttcg tgcgtcagag cgaagagcag atttcgatgg
    12121 tcattcttga agctaatgcc catcaactgc tgttaccgac tgacgatgaa gggcaaaaca
    12181 acgttaccga aaactatttt ctccactgat aattcctttc gagcacgcag tcgctgccgc
    12241 agtgactgcg cgctgcaaaa ttatctgctg tttttaacct tttcttaaag attatttcac
    12301 ttctcttgtg tcgatttggc tttatcacat agagcaaata cgcataaaaa tttgttaaat
    12361 accgtttttt aatccgagct atagtctcaa accctggcta aagttattct tgcgatgctt
    12421 ctatatagcg agcagtgctg gccgggagag agttctcttt tcttacaccg cgccgataaa
    12481 aaaatatgca cgtttattgc atatctttca gtgtgacaac ttttgttcgt ttgttaacga
    12541 actttcagaa ggaaagagat gtgaccgcct taaataaaaa atggctatcg ggtctggttg
    12601 cgggtgctct gatggccgtc tctgtcggca cgctcgcggc tgaacaaaaa acactccaca
    12661 tttataactg gtctgattat atcgcccctg acacggtggc caattttgaa aaagaaaccg
    12721 gtattaaagt cgtctacgat gttttcgact ctaacgaagt actggaaggc aaattaatgg
    12781 cgggtagcac cggctttgac ttggtggttc catctgccag ctttctagaa cgccagttga
    12841 ctgcgggagt tttccagccg ctggacaaaa gcaaattgcc ggagtggaag aatctcgacc
    12901 cggagctgct gaagctggtc gccaaacacg atcccgacaa taaatttgct atgccctata
    12961 tgtgggcgac gaccgggatt ggctataacg ttgataaagt taaagctgtg ctgggcgaaa
    13021 acgcgcccgt cgatagctgg gacttgatcc tcaaacctga aaatctggaa aaactgaaaa
    13081 gctgcggtgt ctctttcctg gatgcgccag aagaagtttt tgctaccgtg ttgaattatc
    13141 tcggcaaaga tcccaacagc actaaagcgg atgattacac cggaccggca accgatctgc
    13201 tgttaaagct gcgcccgaac attcgttatt tccattcatc tcaatacatt aacgacctgg
    13261 caaacggcga tatttgcgtc gctatcggct gggcaggtga tgtctggcag gcgtcaaacc
    13321 gcgcgaagga agcgaagaat ggagtgaatg tctcgttctc gattccaaaa gaaggggcga
    13381 tggcgttctt tgatgtattc gccatgccag cggatgccaa aaacaaagac gaagcctatc
    13441 agttcctgaa ttacctgctg cgcccggatg tagtagcgca tatttccgac catgtgttct
    13501 atgtcaacgc caataaagca gccacgccgc tggtgagtgc ggaagtccgt gagaacccgg
    13561 gtatttatcc gcctgcggat gttcgtgcga agctgttcac tctgaaagtg caggacccga
    13621 aaatcgaccg tgtgcgcacc cgcgcgtgga ccaaagtgaa gagcggaaaa taatccgcag
    13681 ccttagatgc cgggcgggcg caccacaccc gccggcaatt cgcaccatca tggtgcgctt
    13741 gcacacattc aatgccggag agcagccgtg aatgacgcta tccctcgccc gcaggcgaaa
    13801 acccgtaagg cgctgacgcc gctattagaa atccgcaacc tcaccaaatc ctacgatggt
    13861 caacatgcgg tggatgatgt cagcctgacc atctacaaag gtgaaatctt cgcgctgctg
    13921 ggcgcatccg gctgtggcaa gtccacgctg ctgcgtatgc tggcaggttt cgaacaacct
    13981 tctgccggac agataatgct tgatggcgtc gatttgtcac aggttccgcc ttacctgcgc
    14041 cccatcaata tgatgtttca gtcttacgcg ctgtttccgc atatgaccgt ggaacagaac
    14101 atcgcttttg gcctgaaaca ggacaaacta ccgaaagcgg aaatttccag tcgggtcaat
    14161 gagatgctcg gcctggtgca catgcaggag ttcgccaaac gtaaaccgca tcagctttct
    14221 ggtggtcagc gacagcgtgt ggccctggcc cgaagccttg ccaagcgccc gaaactatta
    14281 ctgctcgacg agccgatggg cgcgctggat aaaaagctgc gtgaccggat gcagcttgaa
    14341 gtggtggata ttctggagcg cgtcggtgtg acttgtgtga tggtcaccca cgatcaggaa
    14401 gaggcgatga ccatggcggg gcgcatcgcc attatgaatc gtgggaaatt tgtccagatt
    14461 ggcgaaccgg aagagatcta cgagcatccg actacccgct atagcgccga atttattggc
    14521 tcggtaaacg tctttgaagg cgtactcaaa gagcgtcagg aagatggctt ggtgcttgat
    14581 tcgccggggc tggtgcatcc actgaaagtc gacgcggatg cctcggtggt cgataacgtg
    14641 ccggtacatg tggcgctgcg cccggaaaaa atcatgcttt gcgaagagcc gcccgccaat
    14701 ggttgtaact tcgcggtggg ggaggtgata cacattgcct atctcggcga tctttcggtg
    14761 tatcacgttc gtctgaaaag tgggcagatg attagtgccc agctacaaaa cgcccatcgt
    14821 catcgtaaag ggttaccgac ctggggcgac gaagtgcgtt tgtgctggga agtggacagc
    14881 tgtgtggtgc tgacggttta aggagcaaag atgaatacac ttgaacctgc tgcccagtcg
    14941 aaaccgccgg gcggatttaa gctgtggttg tcgcagctgc aaatgaagca tgggcgcaaa
    15001 ctggtcattg cgttgccata tatctggttg atcttgctgt ttctgctgcc atttctgatt
    15061 gtctttaaaa taagcctggc ggagatggcg cgcgctattc caccttatac cgaactgatg
    15121 gagtgggctg acgggcaact gtccatcact cttaatctcg gtaattttct gcaactgacc
    15181 gacgatccgc tctatttcga tgcttatctc cagtcgctac aggtggctgc gatttcgaca
    15241 ttttgctgtt tactgatcgg ctatccgctg gcgtgggcgg tggcgcacag taagccttcg
    15301 acccgtaata ttttattact actggtgatc ctgccgtcgt ggacctcgtt tctgatccgc
    15361 gtttatgctt ggatgggaat attaaaaaac aacggtgtgc tgaataattt tctgctgtgg
    15421 ctgggggtta tcgatcaacc gctgaccatt cttcatacca atctggcagt ttatattggc
    15481 attgtttacg cctacgtgcc gtttatggtg ctgccgattt ataccgcgtt gattcgtatt
    15541 gattattcgc tggtggaagc agcgctggat ctcggtgcac gaccgctgaa aacgttcttt
    15601 actgtgatcg tgccgctgac taaaggtggg attattgccg gatcgatgct ggtgtttatc
    15661 ccggctgtgg gcgagtttgt gatcccggaa ctgctcggtg gcccggacag catcatgatc
    15721 gggcgcgtgc tatggcaaga gtttttcaat aaccgcgact ggccggtggc ctcggcggta
    15781 gcgatcatca tgttgctgct gctaattgtg ccgataatgt ggttccacaa acaccagcaa
    15841 aaaagcgtgg gagaacacgg atgaataatt taccggtagt tcgttcgccc tggcggattg
    15901 tgattttgct gctgggcttt acctttctct acgcgccaat gctgatgctg gttatctatt
    15961 cgtttaacag ctcgaagctg gtgacggtgt ggggcggctg gtcaacgcgc tggtatggtg
    16021 agttattgcg cgatgacgcg atgatgagtg cggttggttc aagcctgaca attgctgcct
    16081 gtgcggcaac ggcggcggcg atcctcggga ctattgcggc ggtggtgctg gtgcgctttg
    16141 gcaggtttcg cggatcaaat ggctttgcct ttatgatcac cgcgccgcta gtaatgccag
    16201 atgtcatcac gggcttgtcg ctgttgttat tattcgtcgc gcttgctcat gccattggct
    16261 ggcctgcgga ccgcggtatg cttaccatct ggctggcgca tgtcactttc tgtacggctt
    16321 atgtggcggt cgttatttcg tcgcgtctgc gggaactgga tcgctcgata gaagaagccg
    16381 cgatggatct cggtgcgacg ccgctgaaag tgtttttcgt cattacgcta ccgatgatca
    16441 tgcccgcgat catttctggc tggttactgg cttttacttt gtcgcttgat gatctggtga
    16501 tcgccagctt tgtttccggg ccgggagcca ccacgttacc gatgctggtc ttttccagcg
    16561 tgcggatggg ggtgaatccg gaaatcaacg ccctggcaac attgatcctc ggcgcggtcg
    16621 gaattgtcgg atttatcgcc tggtatctga tggcgcgcgc agaaaaacag cggatacgcg
    16681 acatccaacg tgcaagacgt ggctgaagac actaaaattt gccaacctgg ctacataatg
    16741 ccgcgcatgt cgcggcattg ttttcatgga agacgaaacg ttgggatttt ttaagaaaac
    16801 atcttcatct catgctcgcc tgaatgtgcc tgcgctggtg caggtggcgg cgctcgccat
    16861 tattatgatc cgtggcctcg acgtgctgat gattttcaat acgctgggcg tgcgcggtat
    16921 tggcgagttc attcatcgca gcgtacaaac ctggagttta acgctggtct ttttaagcag
    16981 tctggtgctg gttttcattg agatctggtg tgcgttttca ctggtgaaag ggcgtcgctg
    17041 ggcgcgctgg ctatatctgc tgacacaaat caccgccgca agttacttgt gggcggcttc
    17101 gctggggtac ggttatccgg agctgttcag cattcccggt gaatcaaaac gtgaaatctt
    17161 ccatagcctg atgctgcaga agctgccgga tatgctcatc ctgatgctgc tgttcgttcc
    17221 ctcgaccagt cggcggttct tccagttgca ataatgtgta taatcgtcgc ccctgatgat
    17281 gtgaaggtca atgtatgcag tgcgcacttt acgacgcggg tcgctgtcgt tcctgtcagt
    17341 ggataacgca gccgattcca gagcaactct ccgctaaaac cgccgatctt aaaaatctgc
    17401 tcgccgattt tccggttgag gaatggtgcg cgccggtgtc aggcccggaa caagggtttc
    17461 gtaataaagc caaaatggtg gtgagtggta gcgttgaaaa aacactgctc ggtatgctgc
    17521 atcgcgatgg cacgccggaa gacctttgtg actgcccgct ttatccagcc tcatttgcgc
    17581 ccgtttttgc ggcgctaaaa ccgtttatcg cccgagcggg gttaacgccc tacaacgtgg
    17641 cgcgtaagcg tggcgaactg aaatacattc tgctgactga aagccagagt gatggcggca
    17701 tgatgctgcg ctttgtactg cgttctgata ccaagctggc gcaactgcgt aaggcgctgc
    17761 cgtggttaca cgaacaacta ccgcagctga aagttattac cgtcaatatt cagccggtac
    17821 atatggcgat tatggaaggg gagacggaga tctacctgac cgaacaacag gcactggcgg
    17881 agcgttttaa tgacgtaccg ctgtggatcc gtccgcaaag tttcttccag actaatccgg
    17941 cggtcgccag ccagttgtac gccaccgcgc gcgactgggt acgacagctg ccggttaaac
    18001 atatgtggga tctgttctgc ggtgtggggg gctttggttt acactgcgcg acgcctgaca
    18061 tgcagttaac cgggatcgaa attgcgccag aggccattgc ctgtgcaaag cagtcagccg
    18121 ctgaactggg cttaactcgt ttacaatttc aggcgctgga ctccactcag tttgccactg
    18181 ctcaggggga ggtgccggag ctggtgctgg ttaacccgcc gcgccgcggc attggtaaac
    18241 cgctgtgtga ttatctttca acgatggcac cgcgttttat tatctactcc agctgtaacg
    18301 cccaaaccat ggcgaaagat atccgcgaac tgccaggtta ccgcatcgaa cgggtacagc
    18361 tttttgatat gttcccgcac accgcgcact atgaagtgtt gacgctgctg gtgaagcaat
    18421 aaaaaagcca caggtgcggc ttcagattgc tgatcaggtg cgctttgttc atgccggatg
    18481 cggcgtgaac gccttatccg gcctacaaaa tcatgctaaa tcaatacatt gcagagttga
    18541 tgtgggccgg ataagcgcag cgcatcaggc aatcttactt ttgtcatcag tctcaattac
    18601 tgcggaaacc actggtcatt gattttctga tatgtaccgt cagctttaat tgctgccagc
    18661 gcgttattca gtttttccag cagagctttg ttatccggac gtacagcgat acccaggccg
    18721 gtgccgaagt attgcggatc ggtcactttt tcagtcgcaa cgcccagttg cggattggtt
    18781 ttcagccatt cgtttaccac cgcggtgtca ccaaataccc catcaatacg accatttttc
    18841 agatcgataa aggcattctg ataactgtca taggagacag ttttcacttc cgggtgctga
    18901 tcctgaatat atttctggtg cgtcgtaccg ttttccatcc caatgcgttt gcctttcagg
    18961 tcggcaaacg ttttgtaggt atcttttttg gaaatcacga cggctgagtt ttcatagtag
    19021 ggtgtggtaa acgaaacctg cttgctacgc tccggggtga tatccatacc ggagattacg
    19081 gcgtcatatt ttctgaattt cagtgacggg atcaggctgt cgaacgcgtg attagtaaaa
    19141 gtacattctg cctgcatttg tttgcacaag gcttttgcca gatcgatatc aaagccgaca
    19201 atctcattat tagcacctat agattcaaag ggtggatagg tggctgaaac gccaaaattg
    19261 attttctctg cggcagaagc accgaaagta aaggaagcaa gtaaagcggc aagaactaac
    19321 tttttcatga tggaactccc gtctgtcaat cttatgattt ttggccgtgt ctgcggcatg
    19381 ggataacaat gccatcaagt gaatttatat gcaataaaca tgattaaata atttaaatga
    19441 aataaaaaaa gacggataac ttagtgagtt gtccgtcttc attataagaa tttatgcact
    19501 atgtaggccg gataaggcgt ccacgccgca tccggcacag gcaccgtgct gatgtctgat
    19561 gcgacgctgg cgcgtcttat cagacctaca aacccccccg gcgaatgtac gcagctacat
    19621 taattccgcc gttcgaatgc cagtgctttg cgctcgatca gacgcatcat cagcgtcagc
    19681 aggccgttga cgaccaagta aataatccct gccgcaccga acaccattac atcgtaggtg
    19741 cgtccgtaca acaactggct gtatcccatc acttccatca gcgtaatggt gtaagccaga
    19801 gaggtacttt tgaacaccag caccacttcg ttggaataag aagagagcga gcgtttaaag
    19861 gcatacggca gcaggatcgc cagcgtatct tttttgctca ttcccagggc gctacaggac
    19921 tgccactgac cttctgggat cgcacgaatt gcaccgtaaa acagctgcgt ggtatacgcc
    19981 gcactgttca gcgataacgc aatcagcgca cataaccacg gttctgacaa caaatgccac
    20041 agtgccggat actcctgcaa cgtcggaaac tggcccggcc cgtaataaat caggaagatc
    20101 tgcaccagca gcggcgtacc ggtaaacagc gtgatataac cccgcaccag ccacaccagc
    20161 accggcgttt tcagcgtcag gatgatggta aaaatcaatg ccagaatcag tgccacaatc
    20221 agcgaggcaa cggttagcgt caggctggtg tgcagccctt tcatcagctc gggtaaatac
    20281 tcaaacatta gctgggcctc cgctcaaaac gtgtcgcgcg caggtcaatg cgtttgagaa
    20341 tgtactgact gagcagggtg atcaccaggt aaatcgccgc cgccacaatg taccaggtaa
    20401 atggttcctg ggtacgagta gcaatgcttt tggtttgtaa cattaaatca ttcacactaa
    20461 tcaaactgac cagcgcggta tctttcagca gcaccagcca ctggttaccg aggccaggca
    20521 gcgcatgacg ccacatctgc ggcatcacca gacggaaaaa gatagccgat ttcgacagcc
    20581 ccagcgcctg accagattcc cactggccca ccggtacagc tttcaacgcg ccgcgcagcg
    20641 tttgcgaggc ataggcggca tacagcagtg acagagcgat gacaccacaa aggaacggac
    20701 tcacgtcgaa gttctcaatg tccatctgca ccgggatctg cacgaaccca agattgatag
    20761 tgaagccatc cgaaagcgtc agcagtaact gcgaggagcc aaaatagata aacagcacta
    20821 ccagaatttc tggcaggcca cgcagaatgg ttaccagcgc tgaacctgcc cacgcgacag
    20881 gacgccattt cgctgactcc cataccgcaa agaacatcgc cagcgccagc ccgacaatca
    20941 atgcacaaac ggcaaggccg acggtcatcc cggcggcgct tgctaaagga aaaaattcat
    21001 tcatcaggaa ttacttctgg aaccatttgt tgtagatggt ttcgtaagtg ccatctttct
    21061 tcactttttc cagcgcagtg ttgagtttct gctgcagttc agtgtttccc tgacgtaccg
    21121 cgatgccgag gccagtaccg aaataatctt tatcggtcac tttatcgccc actgccgcca
    21181 gttttgggtt atctttcagc cactcagtga ccactgcggt gtcaccaaat acgctgtcga
    21241 tacgcccgtt ttgcagatcc agttttgcgt tctggtagct gtcatacgga acggtggtga
    21301 tttccgggtg cttatccata atgaacttct ggtgtgtcgt cccgttctga acgccgactt
    21361 ttttgccttt cagctgatca acactggtgt atttgccttg ctgacccaca aacagggcag
    21421 agttgtcata gtacggggta gtaaacagta cctgcttttc acgctccgga gtgatatcca
    21481 tgcccgccat cactgcttct acgcgacgga atttcaggct tgggatcagg ctgtcaaacg
    21541 cctggttaga gaaagtacag gttgcatcaa tctctttaca cagtgcctgc gccaggtcga
    21601 cgtcaaaacc aacgatctgg ttgtttgcat caatcgattc aaacggagga tagggggctt
    21661 cggtagcaaa acgaatggtt tcggcagctg tggcggaaag actaaaacct gcaattaacg
    21721 cggcaatcag aacttttttc attgttgtta tcccgaatct tagtgagaga gatagttttt
    21781 aaatgcttcg gtttgcggct cggtaaagca gctcgcgtcg ccttgttcta cgatatgacc
    21841 attttccata tacaccactc ggctggcggt tttacgcgcc acttcaactt cgtgggtgac
    21901 gatcacctgg gtaatattcg tttctgccag ctcacgaatg atgctgacga tttgtgccgt
    21961 aatttccggg tccagtgcgg cggtcggttc atcgaacagc agtacctgcg gttccatcat
    22021 caacgcacgg gcaatagcaa cacgctgctg ctgaccacca gaaagatgca gcgggtaacg
    22081 atcgctataa ggtttgagac gcagacgttc cagcagtttt tctgcacggg ccagcgcctg
    22141 atctttactc aaccccagta cacggcaggg cgcttcaatc aggttttgct gcacggtcag
    22201 atgcggccac aggttgtatt gctgaaacac catgccaacg ttacgacgca aatcgcgaat
    22261 cgctttgtca gagggtgttt tggtgaaatc gaaatggttg cctgcaatgt tgagcgtacc
    22321 ggagcgcggc atctcaagca gattgagtac acgcagcagc gagcttttac ccgcgccgct
    22381 ggggccaagt aacaccagcg tttcgccctg tgggcaatcc agcgtgatat cgaacagcgc
    22441 ctgatgcgcg ccgtagaagc aattaatgcc gtttaattga atactcattg acactcgtat
    22501 actggcagtc tgatagctat tgaggtcgaa gatagtacct ttaacagaat aattatgcaa
    22561 tatttatgct ttaaaagtta aaagcaaagc gcattattca ataaacatag cacaaaataa
    22621 cgggggcggt ggtcggtgag cataaatgtc ggcattcctc acgaaatgcc ggataattta
    22681 cggggtttat tggttgatca aggcgttagc gattctcgat ggactgacgg agcgtacccg
    22741 ccgtggcatg aacgctaccg cctaagtagc gcacatcgtc gatgacccaa cactggcctt
    22801 cctgaatcat taacacttca tcctgccagc cctggtcacc ctgtttgaga tccacccgca
    22861 atggaatgtt acgggcatca cgattaggga tagtcgatgc actggcaacg tgggcgctgt
    22921 ctggcaaggt ggttcgactg gagaacggat cgctgcttaa caattcacga tgactattat
    22981 cgcggctggc atcgctaagt tgggttgcca gtttgtcact caaatagggg cgcagggcgg
    23041 taatgtcatt gctgcgatgt tgaatgcggt agtcgtagaa ttgttgagcc acactgtcag
    23101 gccctccctg gacacaggaa ccggtgcggg ggccgttatc tttataagct ggcgtgacgg
    23161 tggtacaggc actcagcagc agtgcacatg gaataagtag agatagtgaa cggtagcgca
    23221 taatcatttc cttattagct tgctgtctgc gtcacactac gaccaaacgc tatcagatta
    23281 tttaatacat tgtttttaat acaattctga aacaatctga acacctgatt gggtttgagt
    23341 ccattgctgg cgacccactc aaggttgctc gtagctgact tacttaaatt acccaaacaa
    23401 tagacagtgt aaaccatttg tgaggttgag cgtgttatat ggctgaaatg atcatttaag
    23461 gtaaatatga tagttctgaa atggtggtat aaatcctcct gtagaggtta agagcatctg
    23521 caagaagcca gtttcacatt tataacgcgt gataaatgaa tgcggttatt gtctaatcat
    23581 tacaaaattt ttcacatgcg ataagagatc ctgcgattaa gatgattgcc aaaattacgt
    23641 tgtatttttg aaaatgagcg aaatttgata gtcccagctt tgactctcga taatattatt
    23701 tatgctacaa ttgtgggtaa agaatttaag ctaatgaatt tgagtgaaga ataaattaaa
    23761 ttgcttcagt atttcgttaa ttaatataaa tacaaaaggc gttgtgcttt tataattata
    23821 aagcgcgagg agttcggtgg attatcgttt ttcttgggtg atttttaata tgataaacaa
    23881 tatatctgat caagcatcat catttccagg cacacaacta aatcaaagtg ataatttcct
    23941 ggattcactg agggaatttt tcgcgatatt aaatcccagt cgtaagggtg agttgtctac
    24001 atgggatacc atctacttgc atctgatatt agcaataaat gctgacagtg atctcataaa
    24061 aaatgatgtg ttgctcgcgg aaaatattcc atcagcgaat taccaattta atacattttt
    24121 tagcaatact ttcgagattg atgttaaaaa atatctgggt aaatcagaag ataacgaagt
    24181 tgaaataaaa gcgggtaatg aaagaatctc tattggaata cgtaatgtat cgaatggaaa
    24241 acttgaaaga caacagtttc ttttcccact ggactatgag aataaattac aagaacaatt
    24301 agataagtat tttacgattg aatcgcaccc attattatac aggtatacca ttggtagtaa
    24361 gattgcgaat gtgatttttg aaaagttata cagtcgtatt gactttaaca aagaacaata
    24421 tatatccttt ataaaggacg catttattca tttttatgat tatagcaggc gatatgctat
    24481 tagtgagaat atcgataaag acgctgtaac aaacaatatc gcattgatga gcacttttta
    24541 tgacagtgat aatacgtcag gtgaggtttt aaataatgat tttacagaag aagagtcatt
    24601 tgaaactgcg ttagatgtag agcatgcaat agtgcttgga tttgctgatg ataattttga
    24661 aacaaaacct gttcattatc aggatttatt aacacgattt agtgcctttc aagataccgt
    24721 gtttaatctg tttcctgaaa tgcactcttc gcattaccac gatatttgta gcgtaagtgt
    24781 ggatatgaca aagggaacgc aatgcatgat tcacttgatg gtgaatgaag aagttttcat
    24841 gtctctgcct gttccagttg ccacgatggt acgtgaagat gcaagtaatt tagtcaatct
    24901 taaaacacta ctcaatgatg gttgtttcat taaatatagt cattttaatg acgtggcatt
    24961 aataaagcaa aacataagta atctctattt atcacacact gttgtgaatg aaagcatatt
    25021 gaaaaaatgt tgttttgaaa acggcagttt aggtgatgtt aaaataacaa atagcaatgt
    25081 aataaacagt gcattcaaaa atatttcgtt tagatctgtt aaaattaata acgtaaatac
    25141 tcattcttta aaatttatta actgcaattt tttcaatgtt gatatgataa gagttaatct
    25201 cagcaaatgt ctttttcatg aatgtagtat gcatggtgtc aagataaaac cctggttgcc
    25261 tgtaaaatgg acaaaagaat taataagtga ttatttgtat ggctgtttac tatctctgta
    25321 ctctatctgt gcaagggata tatataatat gaacgccggg aataacgtga aggttgctgc
    25381 ggatgctttc cttgaaatta ttttctctct taaaaataaa tattgtatta aactattaag
    25441 cgctcaggat cgggcgttta tatacgagtt tgcaagaatg atcttcgctt atattaatga
    25501 caaaagcatt gagatcttat tactttcttg ttttgcggcg gctgaccaaa aagcaatcca
    25561 gcggtataga cctcagtcac aagatgggga agactttagg agtcatttac aatataagtt
    25621 gcctctttcc gctcattaat gttgttggtg caacataata agacaggtaa gcggtaccct
    25681 ataatgaaac caatatcggg ttatgcaacg gataatcgta aagacttaag tgggtgtttt
    25741 tgacacaatg agatgatttc ctaaactgat caaaaccttc ttcccaacat ggcatatcag
    25801 tccgataatg ctttagagat cgaaggctta acaaacaagg agatcgatca tgcaattttc
    25861 tacaacccca actctggaag gcctgaccat cgttgaatat tgcggtgtgg tgaccggcga
    25921 agcgatttta ggtgccaata ttttccgtga tttctttgcc ggtatccgcg atatcgttgg
    25981 cggacgttcc ggtgcgtatg aaaaagaact gcgtaaagca cgggagatcg cctttgagga
    26041 attaggctcc caggcgcggg cgctgggggc cgatgccgta gttggtattg atatcgacta
    26101 cgaaacggtc gggcaaaacg gcagtatgct gatggttagc gtcagcggta cggcggtgaa
    26161 aacgcgtcga tgagaagagt cttctggctg gtcgctgccg ctctgttact ggcagggtgt
    26221 gcaggcgaaa aaggcattgt cgagaaagag ggatatcagc ttgatacccg acaccaggcg
    26281 caggcggcgt atccgcgcat taaagtgctg gtgatccact acaccgcaga tgattttgat
    26341 agctcgctgg cgacactgac tgataagcag gtcagctcgc attatctgat ccctgcggtg
    26401 ccaccgcgat acaacggtaa accgcgcatc tggcaactgg tgccggaaca agaactggcc
    26461 tggcatgcgg ggattagcgc ctggcgcggg gcaacgcgcc ttaacgacac ctctattggc
    26521 attgagctgg aaaaccgtgg ctggcaaaaa tcatccggag tgaaatattt tgccccgttt
    26581 gaacaggcac agattcaggc gcttattccg ctggcgaaag atattattgc ccgttatcac
    26641 atcaagccgg aaaacgtagt ggcacatgcg gatatcgcac cgcagcgcaa agacgatccg
    26701 gggccattat ttccctggca gcaactggcg cagcagggga ttggtgcctg gccggatgcg
    26761 cagcgggtga acttttacct tgccgggcgc gcgccgcaca caccggtaga taccgcgtca
    26821 ttgctggagc ttttggcgcg ctacggttat gacgtaaaac ctgatatgac accgcgcgag
    26881 cagcggcgcg tgattatggc attccagatg cacttccgcc cgacgttata taacggcgaa
    26941 gcggatgcag aaactcaggc gattgccgaa gcattgctgg agaaatacgg gcaggattag
    27001 cgcggcagtt ttccgtggtc gcgtagccag gcggcagttt tctcgatgcc ttcatccagg
    27061 gtgatgaccg gctgataacc taactcttcc tgcgcacgcg taatatccag cgtaaagtca
    27121 aaattcaact tggaggcgcc gtagtgggtc agcggcggct cttttgctga cttgcggcct
    27181 aaacgctcca tgctgcgggc gatcatatcc agcatcgggt aagggacgga acgaatacga
    27241 cagtcaatat tcaactcgtc gatcagcttc tgcacgatgc tgcgcagtgt gcgatgctcg
    27301 ccgttggtga tgttgtacac acgcccggaa ggtagcttat cgcaggcttc ctggcttgcc
    27361 agccacattg cgtgcacggc attttcatag taggtcatat cgaccagcgc actgccgcca
    27421 tgcggtaaca gaatactgcc gtagtggtgc atcatatgcg ccagacgggg aataaagact
    27481 ttatcgtgcg gtccgaacag actttgtggg cgcagaatag taaagcgcgt ttgtggattc
    27541 gcctgcgaaa gcatattgat cacttcttcg ctggctgctt tgctgcgggc aaactcgttg
    27601 gcgaagcggt gagggcgaaa atcttcttta atattgcgat ggtggtgata atcgaagtac
    27661 agggaggggg aagagatatg aataaagtta cgcacacccc aggcgacagc ccattcaccc
    27721 aggcggcgag tggcgcgaac gttagccaga tcgaaagcct gttgtgtccc ccagggtgag
    27781 gtaaagctgg agcagtgcca cagcgtatca atgcccgcga gcatcacttt agcttgtgat
    27841 gaaaccagct cggtcagatc cgtcggaaca aactctgcgc ccattttttc cagcaattta
    27901 cccattgctt cgttgcgacc gctcgcacgc acgctgatgc ctttctggca taaaaactct
    27961 accgcgtttc gacctaagcc gctggtggcg ccggtaacca gtaccttcat atcaatccac
    28021 tgttgttgag aaaatgacgt gcgcattctt ccgtgatttc ccccatgatg caatgggaaa
    28081 catgaaagaa taacgcaggt tttgtcgatt aatctgtgct ttgttctgcc agtctggcga
    28141 tttgttttgc cattccgcga aaaataaaca gatgcgcggg gatcatcaat aaccagtaaa
    28201 acagccccgg cataccgtgc ggatgccaga aagcgcggac atcgatagta cgatagtcac
    28261 ctttatcttc cagggtaaaa cacagtcgtc ccagccccgg cgctttcatg ccaaataaca
    28321 acgtaagttg ctttttcggt tcaacgacaa tcactttcca gctatccacc gcatcgccgg
    28381 tctgtaaata ttcgcgctcc gggcggcctt tcgccagatt atgaccgatc gcgcggtcca
    28441 tcaacgcccg tgtctgccac aaaatattgc caaagaaata acgctcttta ccgccgattt
    28501 ggttcaccac cagccataaa gccgcaaggc tggcggacgt tttaacggta aaccccgcct
    28561 gtttggcgaa ataaccgtac tccggtcgcc agcgggcaaa ggcctgagcg tcgtagcccc
    28621 agtcgctgga gttgaccagt ttttcttcct ctttcaacgt gctacgtacc gcgtcatcga
    28681 aagcgatcag ccgttgtggg atgagtgcac gtagcgcggt atcatctgcc agcagatcgt
    28741 gtttcagccc ctgaatcaac gccctggcgg tggtgggcgg tacggaagta atcacattga
    28801 gaaaccacac cgaaatccag cgggtgggga gggggatggg gatcaaccag cggcgcttac
    28861 cgctcaccgc cataaaatgt tcaaactgtt gctgataact gagcacctct ggtccggcgg
    28921 cttcgaagat gcggtgttcg ttggctggat ggtctaacag cgccaccaga tagtgcagca
    28981 agttttccag cgcgatgggc gtggtgcgtg aacgtaccca gcgtggcggc gttaacactg
    29041 gcaggttgta gaccatatcg cgcatgactt cgaacgccgc tgaacctgcg ccaacgataa
    29101 ttccggcccg aagttcggtc actggtacat tcgcttcacg aagaatgtcc gccgtagcct
    29161 gacgagctcg cagatgatcc gactgctcat gtggcggggc ctgcaacgaa ctgagaaaga
    29221 ttaattgctt aactggtact tcacgtagcg catcgcggac gttgagagcc agctggcgct
    29281 cctgagcgat aaaatcgccg ccttcgccca tgctgtgcac cagaaaatag acggtatcga
    29341 tatcctgcaa cagggccgga aggttatccg gccagctgag atcgacttta tggcaactga
    29401 cgtttgccag ttgcagcttt gcaagcctgt cgacatgacg tgccgccgct aggatctgat
    29461 gcccttgctg gctgagtgtg cgcaccagat gctgaccaat gtagccactg gcaccgagaa
    29521 ctaaaatgcg ttgcggcacg tctctctcct taacgcgcca ggaatgcgcg ccagtgggcg
    29581 gcgacttccg ccagttgttc gcgcgagacg tcaagatgcg tcaccaggcg gacaatcggc
    29641 gaggcgttaa tcagcacgtt tctcgctttc atgtattcgc ctaacgcggc agcattttct
    29701 tccccgacgc gaacaaacag catattagtg tcctgacgca tcacatccgc gcctgcttca
    29761 cgcagttgct ccgccatcca ggcggcgttg tcgtgatctt cctgcaaccg tgcgacgtta
    29821 ttttccaggg catacatccc ggcggcagcc agaatcccgg actgacgcat cccgccaccg
    29881 gccattttcc gccagcggat ggcacgttta atgtaatcac gattaccgac gagtaatgaa
    29941 ccgactggcg tcccaagacc tttcgacagg caaatggtga acgaatcaca atattgcgtg
    30001 atctctttca gttcgcagcc gtaagccacc acggcattaa agatgcgcgc accgtcaaca
    30061 tgcagcgcca gattgcgctc gcgggtaaat tcccatgctt ctttcaggta ttcacgcggc
    30121 agcactttgc cgttgtgggt gttttccaga ctgagtaatt tggtgcgggc gaaatggata
    30181 tcgtcgggtt tgattttcat cgccacttta tccagcggta gcgtgccgtc ggcagccgcg
    30241 tcgatgggtt gcggctgaat actgcccagc accgctgcgc caccggcttc aaacagatag
    30301 ttatgcgcgg cctgaccgac aatatactct tcgccacgtt cgcagtgact gagcagagcg
    30361 accaggttgg cctgagtgcc ggtaggcaga aaaatggcgg cttctttacc ggaaagctct
    30421 gctgcatagt cctgcagagc attaacggta gggtcgtctc cgtaaacgtc gtccccaacc
    30481 ggggcggcca tcatcgcttc gagcatggcg cggctcggtc gggtaacggt atcactgcgt
    30541 aaatcaatca tggcatgtcc ttattatgac gggaaatgcc acccttttta ccttagccag
    30601 ttcgttttcg ccagttcgat cacttcatcg ccgcgtccgc tgataattgc gcgcaacata
    30661 tacaggctaa aacctttggc ctgttcgagt ttgatctgcg gtggaatggc taattcttct
    30721 ttggcgacca ccacatccac caacaccgga ccgtcgatag agaaggcgcg ttgcagggct
    30781 tcatcaactt cagaggcttt ttctacacgg atacccgtaa tgccgcacgc ttcggcaatg
    30841 cgggcaaagt tggtgtcgtg cagctcagta ccgtctgtca ggtaacctcc ggccttcatc
    30901 tccatcgcca caaagcccag cacgctgtta ttaaagacga taattttcac cggcagtttc
    30961 atctgcacta ctgagaggaa atcgcccatc aacatgctaa aaccgccatc gccgcacatg
    31021 gcgaccacct gacgttccgg ctcggttgcc tgcgcaccca acgcctgcgg catggcgtta
    31081 gccatcgaac cgtggttaaa cgaacctaac aggcgacgct tgccgttcat tttcagataa
    31141 cgtgccgccc ataccgttgg cgtgccgacg tcacaggtaa agatggcgtc atcggcggca
    31201 aaatgactaa tttgctgcgc cagatattgc gggtgaatgg ctttctcgct cggtttagct
    31261 aaatcgtcca gccctttgcg ggcatcgcgg taatcttcca gcgctttatc cagaaacttg
    31321 cgatcggttt tttcttccac cagtggcagc aatgcgcgca gggttgattt gatatcgccg
    31381 accagcgcca tatctacctt gctatgcgcg ccgatgctgg ctggattaat atcaatctga
    31441 ataattttgg catcggtcgg gtagaacgca cggtagggga actgagtacc gagtagcact
    31501 aacgtgtcgg cgttcatcat ggtatggaaa cctgacgaga atccgattaa cccggtcatt
    31561 ccaacatcat acggattatc gtattcgaca tgctctttac cgcgtagggc atgaacaata
    31621 ggtgctttaa ttttcccggc aaactcaact aactctttat gcgcccccgc gcagccgctg
    31681 ccacacatca gggcgatatt gctggaataa cgcagcagtt gcgccagttt gcgtaactct
    31741 tcttcttccg gcatcacgac tggctgtggc gcatgatacc agtgcgtggt tgccccttct
    31801 ggcgcaggtt ttaacgccac gtcgcccggt aatacaacaa cggaaacgcc acggttaagc
    31861 accgctttgc gcatagcaat tgccagcact tgtgggatct gctccgggct ggaaaccagc
    31921 tcgcaatagt gactacattc gcggaatagc tcttgtgggt gggtttcctg gaaatagccg
    31981 ctgccaattt cgctggaggg aatatgagcg gcaatcgcca gtaccggaac gttattgcgg
    32041 tggcaatcga ataggccgtt gatcaagtgc aggttgcctg ggccgcacga tccggcacag
    32101 accgccagtt ctcctgaaag ttgtgcttca gcgccagcgg caaacgccgc cacttcttca
    32161 tggcgggtgg acatccactc gatggtgccc atgcgattaa gactgtcgct aagaccgttc
    32221 agagagtcgc ccgtgactcc ccagatgcgt ttcacgcctg ccgattcgag tgttttggcg
    32281 atataagctg caaccgtttg tttcatggtt ctccatctcc tgaatgtgat aacggtaaca
    32341 agtttagttc atctgacgga gggggaaggg atgggagaga aaggaggcac taacggttaa
    32401 atagcccgat gaaaggaata tcatcgggca taaggcgatt atgcgagaac caaatccccc
    32461 tgcggatggc aggagcaggc cagtacgtaa ccttcagcga tttcggcgtc ggtcagcgtc
    32521 attgtactgc tcaccgtata ttcaccggaa accacttttg tcttacagca gccgcaaaca
    32581 cccgcacggc aggcagcgac aaccggaacg ttattgcttt ccagcgcctc cagtagcgtg
    32641 gtgccaaccg gggcgtaaaa ttctcgtgcc ggttgcagtt tggtgaattt cagaccgctg
    32701 gtcgccgctt ctgctactgg ggtgaagaat ttctctttaa agaaacgcgt cacgccgagc
    32761 gctttcactt cctgctctac ccaatccata tacggagccg ggccgcaggt catcacggta
    32821 cgtgaagcta agtcaggtac atctgccagc agttcgcgag tgaggcgacc agcgataaag
    32881 ccttcggtaa cgttattttc tgccaccagc gttaccggat agttacgcca ctcatcggcg
    32941 aaaataacat cctgcggcgt acgcacgttg tagatcaccc gcacatcggc ctgtggacgg
    33001 ttcttcgcaa gccagcgacg catcgacata atcggcgtga cgccgcagcc tgccgccagc
    33061 aacaggaatt tatcttctgc tttatcgtcg caggtaaatt cccccatcgc gtccgaaagc
    33121 cagagataat caccgcgttt tacatcgcgc gtcagccact gggagccgac accgtcatca
    33181 atccgccgca cggtcagggt gatatattca ctcacgcctg gcgtggagga aatggtgtaa
    33241 gcacgcagcg tttccgctga gtttcgcacg ctgaccagtg catattgccc ggcgcgatat
    33301 gggtagtgat cgtggcaaat cagggaaatc gtccacacat ccggcgtttc ttgcgtaatg
    33361 tgatgaacct gtatccgcca cgggcattga ttcgttggca tcgtcatcga caaactcctt
    33421 acgcgttcaa cagttgcttc atgtcttctt caacagtggt gatagaacgc aggccgaatt
    33481 tctcgttcag caccgccagc aggtctggtg tcaggaaacc aggtgcagtc gggccggtga
    33541 cgatattttt cacgcccaga gaaagcagcg tcagcagaat gacaatcgct ttctgttcaa
    33601 accaggagag caccagcgac agcggcagat cgttcacacc acagcccagt ttctctgcca
    33661 gagtgacagc cagaataatt gctgagtaag catcgttaca ttgacctgca tctaccagac
    33721 gcggcagacc ttcgatatcg ccaaactcaa gtttgttaaa gcgatattta ccacaggcga
    33781 gggtcaggat caggcagtca tccggcacgc tggtggcgaa atcggtgaag tagtggcgct
    33841 cgccgcgtgc gccgtcacag ccaccaagca ggaagatatg acgcagtttt tcacggctaa
    33901 ccagatcaat cagcgtatcg gctgcaccaa gcagcgtctg gcgaccaaag cccacggtga
    33961 tcaggtgagg aatttcgctg tacgggaagc ccgccatttg ttgcgcctgg gcgatgaccg
    34021 cagagaaatc ttcgccatcc agatgacgca cgccaggcca gccaacgatg ctgcgggtcc
    34081 agatacgatc gtcataagcg cctacggttg ggtcgatgat gcagttcgag gtcatcacga
    34141 tggggccagg gaaacgagcg aactccactt gctgattctg ccagccgctg ccgtagttac
    34201 cgaccagatg cttgaattta cgcagctccg ggtagccatg cgcaggcagc atttcgccgt
    34261 gggtgtagac attaacgccc gtgccttcgg tctgttccag caggttgtag agatctttga
    34321 gatcgtgacc ggaaatcaga atgcatttac ccgccgtcgc tttgacgttg acctgggttg
    34381 gcgtcgggtg accgtattta ccggtttcgc ctgcatccag aatgctcatc actttgaagt
    34441 tcatctggcc gatttccatt gaacactcaa gaagcgcgtt catatcggca ggccaggtcc
    34501 ccagccacgc catgatttta tggtactggg cataaatatc gttgtcgtat tgaccgagaa
    34561 catgcgcgtg ttccatatag gccgccgcac ctttcaggcc atacaggcac agcagacgca
    34621 ggccgagaat gttttcgcca atcgccgctt tatctttgtt aggggtaaat tctgctgcct
    34681 gacgttgcag ctcgccgaga tcatcgctca ccagttgcag gtcagccatc gggttatcga
    34741 cgcgcgcgtt ggcatctaca gccaggcatt gtgctttcag cgcctcgcgc aaggcaatcg
    34801 cttcacgagc gtagccgaca atacgcggag aatcgaagtt aacgttggtc agggttgaga
    34861 aaaaggcacg tggcgcgaag ctgtcaacat cgtggttgat gatgccgtat tcacgcgctt
    34921 ttaccgccca ggcagaaagc ccttgcagcg ccgcgatgag taaatcctga aggtcagaag
    34981 tttccgccgt tttaccacac atcccctgcg cgtatgagca gccgttttct gccggagtac
    35041 ggatagtttg ttcacattgc acacaaaaca tgatcacacc ttttaaagtt atatttaata
    35101 tacatgttta agggtaagac gcttaacgcg gggataaaag ggatttttca tgcaacttta
    35161 agggagattg atttagcgca attttggcgg cagggaacta ccgccagaga ggtattacgc
    35221 agagaaaaag gcgatgagga tcggcactaa caggctaaga ataaaaccgt gaacaattgc
    35281 cgccgggacc atatccagcc cgccagtacg ttgaagaacg ggcagggtga aatccattga
    35341 tgtggcaccg cataagccca gtgcagtaga gcggctgcgg cgaatcagcc cagggatcaa
    35401 cataatagca atcagttcac gggccagatc attaaaaaac gccgcgctcc cgattaccgg
    35461 accaaaagat tcggtcaata aaataccgga aagagaatac cagccgaaac cggaggccat
    35521 tgccagcgcg gtattgatgg ggagatcaag aataaaggcg ttaattaaac caccaattaa
    35581 tgaactgaca accaccacca cggcgacaat cattccccgg cgattaagga caatctgctt
    35641 taaggtcatg ccattattgc gcaactgaat accaacgagg aaaagtagca aaattaacgt
    35701 gtattcactg gcttcggtcg cgtgttgtaa gaaagccagt ccactcagac caatggcaaa
    35761 accaatcact actacgccgc acagttttag cgactccagc gccatcgcaa tacgcgacgg
    35821 gagtttttct tgctgatggt ggttgcgcca cggcaggcct cgctccagcc acatcagggc
    35881 ggcaatatta cacagtaaaa taacggtaat actgacggca gaataatgca gaatcgccaa
    35941 caggttactg gcgaggttat cgagaaacgc cagactgata cccataaaaa agagaataag
    36001 gtaaaccatc cagcttaata gctgattaat agcttttaac gcagcttgtt ggcgaagcgg
    36061 aatgaggtaa cccacaatca ggggaaccag aatgattaac agcccagaaa acatgaaaat
    36121 ccagtccttg caaagaagaa gtcgaaatgc gcgatgacac actactgaaa gcggaagggc
    36181 gagtaaagtt gcaattaaaa ggaaatgtta tgcatcagag gg
//
LOCUS       DAJTSF010000032        35111 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000032 DAJTSF010000000
VERSION     DAJTSF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.032"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="OO335_001686"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="OO335_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR6002111.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="OO335_001687"
     CDS             1207..1869
                     /locus_tag="OO335_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6002112.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="OO335_001688"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="OO335_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6002113.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="OO335_001689"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="OO335_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="OO335_001690"
     CDS             2795..3100
                     /locus_tag="OO335_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6002114.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="OO335_001691"
     CDS             3103..3507
                     /locus_tag="OO335_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6002115.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="OO335_001692"
     CDS             3497..3796
                     /locus_tag="OO335_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6002116.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="OO335_001693"
     CDS             3982..4278
                     /locus_tag="OO335_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6002117.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="OO335_001694"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="OO335_001694"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR6002118.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="OO335_001695"
     CDS             5627..5995
                     /locus_tag="OO335_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6002119.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="OO335_001696"
     CDS             complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="OO335_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR6002120.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7203..7904
                     /locus_tag="OO335_001697"
     CDS             7203..7904
                     /locus_tag="OO335_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6002121.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7927..8091
                     /locus_tag="OO335_001698"
     CDS             7927..8091
                     /locus_tag="OO335_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002122.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8303..9613)
                     /locus_tag="OO335_001699"
     CDS             complement(8303..9613)
                     /locus_tag="OO335_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6002123.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9641..10972)
                     /locus_tag="OO335_001700"
     CDS             complement(9641..10972)
                     /locus_tag="OO335_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6002124.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="OO335_001701"
     CDS             complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="OO335_001701"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6002125.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12683..13072)
                     /locus_tag="OO335_001702"
     CDS             complement(12683..13072)
                     /locus_tag="OO335_001702"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6002126.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="OO335_001703"
     CDS             complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="OO335_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR6002127.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="OO335_001704"
     CDS             complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="OO335_001704"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6002128.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="OO335_001705"
     CDS             complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="OO335_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6002129.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="OO335_001706"
     CDS             complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="OO335_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6002130.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="OO335_001707"
     CDS             complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="OO335_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6002131.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20217..20561
                     /gene="tdcR"
                     /locus_tag="OO335_001708"
     CDS             20217..20561
                     /gene="tdcR"
                     /locus_tag="OO335_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6002132.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20817..21357
                     /locus_tag="OO335_001709"
                     /pseudo
     CDS             20817..21357
                     /locus_tag="OO335_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21379..21726
                     /locus_tag="OO335_001710"
     CDS             21379..21726
                     /locus_tag="OO335_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002133.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21895..22545
                     /locus_tag="OO335_001711"
     CDS             21895..22545
                     /locus_tag="OO335_001711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002134.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(23053..23429)
                     /gene="rnpB"
                     /locus_tag="OO335_001712"
     ncRNA           complement(23053..23429)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO335_001712"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(23462..24607)
                     /gene="garK"
                     /locus_tag="OO335_001713"
     CDS             complement(23462..24607)
                     /gene="garK"
                     /locus_tag="OO335_001713"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6002135.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(24704..25594)
                     /gene="garR"
                     /locus_tag="OO335_001714"
     CDS             complement(24704..25594)
                     /gene="garR"
                     /locus_tag="OO335_001714"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6002136.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(25624..26394)
                     /gene="garL"
                     /locus_tag="OO335_001715"
                     /pseudo
     CDS             complement(25624..26394)
                     /gene="garL"
                     /locus_tag="OO335_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(26410..27744)
                     /gene="garP"
                     /locus_tag="OO335_001716"
     CDS             complement(26410..27744)
                     /gene="garP"
                     /locus_tag="OO335_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6002137.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            28119..29690
                     /gene="garD"
                     /locus_tag="OO335_001717"
     CDS             28119..29690
                     /gene="garD"
                     /locus_tag="OO335_001717"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR6002138.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(29742..30566)
                     /gene="agaR"
                     /locus_tag="OO335_001718"
     CDS             complement(29742..30566)
                     /gene="agaR"
                     /locus_tag="OO335_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR6002139.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            30815..32095
                     /gene="kbaZ"
                     /locus_tag="OO335_001719"
     CDS             30815..32095
                     /gene="kbaZ"
                     /locus_tag="OO335_001719"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR6002140.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            32118..32591
                     /gene="agaV"
                     /locus_tag="OO335_001720"
     CDS             32118..32591
                     /gene="agaV"
                     /locus_tag="OO335_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR6002141.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            32602..33381
                     /locus_tag="OO335_001721"
     CDS             32602..33381
                     /locus_tag="OO335_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR6002142.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            33371..34180
                     /locus_tag="OO335_001722"
     CDS             33371..34180
                     /locus_tag="OO335_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR6002143.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            34267..34701
                     /locus_tag="OO335_001723"
     CDS             34267..34701
                     /locus_tag="OO335_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6002144.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            34698..>35111
                     /locus_tag="OO335_001724"
     CDS             34698..>35111
                     /locus_tag="OO335_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6002145.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         9094 a         9063 c         8388 g         8566 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatat ttccgccagt agtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtaga gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc atcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cggccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg
     6061 cggcgtaaac gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc
     6121 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc
     6181 ttgtgaaagg tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca
     6241 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
     6301 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg
     6361 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
     6421 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
     6481 agcggtatcc gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc
     6541 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
     6601 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc
     6661 cgcccgcccc tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag
     6721 ttgggtattg gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac
     6781 cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc
     6841 agtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc
     6901 gacattggtg aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc
     6961 ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc
     7021 cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa
     7081 tgccctttct ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat
     7141 ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa
     7201 ttatgattac tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg
     7261 cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc
     7321 tgcgtgtgct taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc
     7381 ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag
     7441 gcaatcatgt tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca
     7501 gctatagcga acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg
     7561 atacctgccc gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc
     7621 agcaattaat cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt
     7681 ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac
     7741 gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag
     7801 cggcgctaac ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg
     7861 ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag
     7921 atgatgatga gtaaaaaatc gaccaaaaag cgccagccag cgaagcccgt ggtggcgaaa
     7981 gaacctgctc gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct
     8041 atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt
     8101 gccggatgcg gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa
     8161 acaccccgta ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca
     8221 acaacatcaa caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg
     8281 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
     8341 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
     8401 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
     8461 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
     8521 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
     8581 cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
     8641 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
     8701 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
     8761 ccacaggcat tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa
     8821 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
     8881 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
     8941 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
     9001 gaataaagcg gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg
     9061 tcgttctgga aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt
     9121 gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
     9181 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
     9241 tgcaaggttc ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg
     9301 taattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
     9361 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
     9421 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
     9481 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
     9541 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
     9601 tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca
     9661 gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt
     9721 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
     9781 aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc
     9841 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct
     9901 cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
     9961 ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca
    10021 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
    10081 tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
    10141 cgtagaaaaa gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt
    10201 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
    10261 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
    10321 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
    10381 acacgcccag cgcagcgact accagcagct tggtcagcac catgtcggtc gaaattttga
    10441 ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat
    10501 aaaagggact gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac
    10561 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga
    10621 gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat
    10681 ctttacattc tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg
    10741 ccgggtaacc aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca
    10801 ccgggagaaa aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg
    10861 tgtcagtact gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac
    10921 ccgccggggt cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc
    10981 gctcaatatt ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc
    11041 tacgtttttg caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat
    11101 cttcacaatc aagactttaa aataacggag atggataaga attttctact aaattaatca
    11161 caagagaata ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca
    11221 cccaaggatg aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg
    11281 cgtgatgttt cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc
    11341 actttatcga gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt
    11401 actgctttca cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca
    11461 accggatcgc aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc
    11521 tgcgccggac tgccgcccag tagttcagtt aaccctgccg ctgccattga acaggccacg
    11581 ccaatctccc cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc
    11641 gcgccaatag cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga
    11701 cggaacttat cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt
    11761 gccgttacca cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg
    11821 atccagtcga tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga
    11881 cgcagcgcta cggcacgaca cggcacattg agcggaccag gcagcacgcc ttcagtgttc
    11941 atcccgcgtt caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt
    12001 tccgctttgc tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg
    12061 ttgtaatcac acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt
    12121 tcgacatcgt gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag
    12181 taataggttt tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc
    12241 tcatggcgag gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca
    12301 atatgcgcac cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca
    12361 gggatctcat caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg
    12421 acatccgtgg catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata
    12481 tggctcgtcg cggttaataa gccgctactt tccagccgat caataaaact ttttccggca
    12541 ttcattggcc ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat
    12601 gcactaatca tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg
    12661 cgaccgtccc gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct
    12721 tcacatcttt cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat
    12781 gctcatcgaa gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga
    12841 tgaacacggt catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt
    12901 tgacgttttc gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct
    12961 gcgggcaaac cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa
    13021 cataagggcc gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc
    13081 cggcgtcaga gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc
    13141 agtgcgttga agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc
    13201 gggtgctcga tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga
    13261 ccgccttcga catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt
    13321 ttacgtactg gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc
    13381 ccatctttgg cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct
    13441 ttgcggtcac gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga
    13501 ccgtccggcg tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc
    13561 gactgggtag ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt
    13621 tcaaccaggt cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat
    13681 tcaccgtcga tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg
    13741 gcatatttga tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc
    13801 atagtgcgat agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg
    13861 tcgtgcatgt agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg
    13921 aagtgatcga ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct
    13981 gttttcggcc cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc
    14041 agcgttttcg ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt
    14101 gggctgacgc agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg
    14161 tcattttcat actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag
    14221 gcaatcggta attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata
    14281 gtgtgcaggg tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc
    14341 cccatcccgc cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat
    14401 tccggtgtac gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc
    14461 tgtgcctgct gctcattgag gacgccagct ttaaagtcgc gctcaatgta gatatccagg
    14521 aacgatgccg tgcgccccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga
    14581 taagcgaagt agagccactg caccgcttcc tgtgcattct gcgccgggcg agagatatca
    14641 aagccatatt tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc
    14701 tcctcacgca gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga
    14761 tcggcaaatt gcagttcgcg ttcacgcacc agataactga tgccatacag cgctacgcgg
    14821 cgatagtcac cgataatgcg cccacgacca tagccatctg gtaaaccggt cagcacgcca
    14881 gatttacggc agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt
    14941 ttacgcagat cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg
    15001 aatgaacttt taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca
    15061 tccgtttgca ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg
    15121 gtaattgtgg tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata
    15181 cggatgcctt ccattacctt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga
    15241 aaagattcat cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt
    15301 tcgcttttcc agtccgtacc tttaaagcca agccatgcgt cagcgtacag cttatcgctg
    15361 gtatcaatat ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca
    15421 aattctgcgg gcgcgttaac tttacctaaa tgaatggcat ccaaagcaat cattttttct
    15481 tcgttagtcg gaataacgac gcaaatgaca tgcgcatttt cactggaaac aattcgctcg
    15541 ccaaaagagt tagaacgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa
    15601 tgttccatga ccagacgacg aattaaactt gaattctctc ctattccgcc ggtgaatata
    15661 attccatcca gccgacgtaa tgaagctgca tgtccggcaa tatgacgggc aattcggtga
    15721 acaaaggttt taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga
    15781 acacgtaaat ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg
    15841 cgttccaggt cacccaggct ctggttggtt tggctggcga cccaggacat cgcaccaaag
    15901 tcgacatcgc cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag
    15961 gtgtcaacac tctgaccgtt gcgaactgcg cagattgacg cgccattgcc aagatgcgcc
    16021 acaaccaggc cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca
    16081 tagcggtgcg acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc
    16141 cacggcaggc cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat
    16201 accgccacct gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattt
    16261 gcgtaattat gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacttcatcg
    16321 gtaataatgg cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag
    16381 gccacgctgt catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct
    16441 tcgtagctgt ggtgagccag cggtgctggc tctcccccat ttacggataa gaatgcattt
    16501 tccgagttaa taccgtcggc aatacctgac attaatactt cacaatcgct ggcatcgagt
    16561 acggaaaact taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc
    16621 atctcttttc tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt
    16681 cagcagacca atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga
    16741 cggcgcttta cggatagcat acatcggcaa caggcacagc agggatgcga taatcggtgc
    16801 gcccatggct tcaatcaggt cgaggatgtt cgggttggcg taggcaacaa cccaggtgga
    16861 gcccatgatg aagatcatgc tgagagtatt cagtttaccc agcgacactt tggttttgtc
    16921 acctttataa ccgaacttca gaatcagacc attcaagcct tccagcgtcc ccagatagtg
    16981 accgaagaaa gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt
    17041 aatcgcgaac gttgttttcg taccggtcat ggacgcaaag tggttagcca gataagaaag
    17101 cactggaata ttctgcgctt tggcttccgc catgttggcc ggagacagag taaacaggca
    17161 gctaaaggca aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg
    17221 ggaacattta cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga
    17281 aaccacgaag gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc
    17341 cagccagaca gtgatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac
    17401 ctggtcgata actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct
    17461 ggcgataaac ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca
    17521 gatgacgaaa gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc
    17581 aaatccgagc tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat
    17641 ccacagcagt gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt
    17701 accaaaatgc tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca
    17761 cagacgcgcc agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag
    17821 aagaatcggg atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc
    17881 gattgccgta ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg
    17941 ttttgtctgg ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa
    18001 ttaagcgtca acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga
    18061 aataatactg acggtttttc tattttgaat atattggtct aatttaccgc ttaataatgc
    18121 agcacatgcc agagcgcctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat
    18181 catactgttt ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac
    18241 gatttcgtaa gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt
    18301 agttcggtgc gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc
    18361 tgactgtacg ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc
    18421 agcaattaaa ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc
    18481 cataatttcc agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg
    18541 gataaaaata cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa
    18601 gttatcacca tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt
    18661 tggcgcacct tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga
    18721 aaccccttgc gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc
    18781 ggtcagtgaa cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat
    18841 attttcaaac ttcaggaata tttcaccttt gcaacgttca ctaaaatagt tggagcgagg
    18901 catgcctgtt ttataaattc gcccagccag tcgttgtttc gcttcaataa tatcatcaat
    18961 agcaaccggc agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg
    19021 taggtaaccg taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa
    19081 acaaacaact aaccaacttc tattaattgc cttcgtctac acccattata agatgaatac
    19141 tctttggcta attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat
    19201 accgcggcat attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgatta
    19261 gaaccaaaag gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca
    19321 agattataaa ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt
    19381 ctttgtaacg tagtaagcag ttcgctgtag taccccatat tagtttgtgg caacacccac
    19441 tgttcgttct tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct
    19501 accagcacaa actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct
    19561 gcacttaacg taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa
    19621 gacagttgcg cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg
    19681 atcatccctg acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc
    19741 accgcttcag aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg
    19801 gattcggaac gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc
    19861 cgcaccacta attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc
    19921 ggttgagtta accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc
    19981 tgaaagacta ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt
    20041 aattacctca ttgacggcat gaagtgtatc aaaatgaaat aaacaggata tgtgcgacca
    20101 ctcacaaatt aactttcatt actttccaag gtatcgttat tacaattaaa catctttaaa
    20161 tatcaacaag ttaaagcata aaaatcagca taaaccctga tttttatcaa aaaaaaatga
    20221 cggggataac catattttat ggcgataaca tcattcgtta tgtggttaat acaaaaaagg
    20281 ggctgagacc atatttcaag caattaccgg ataattacca ggcaaaattt gagttaaatt
    20341 taatgagcaa gttctcaaat tttattataa ataaaccatt ttcagcgata aatactgcgg
    20401 cacgtcacat tttttcacgc tatttgttgg agaacaaaca tttattttat caatatttta
    20461 aaatttcgaa tacaggtatt gatcatctcg aacaattgat taacgtcaac tttttctctt
    20521 ctgacaggac gtcattttgt gaatgcaatc gttttccata aattcttctc ccctcatagg
    20581 cgacgaatag cattttgtgt tgaggatcac aaaacgaata attgctggtc gccgcgataa
    20641 ggtcagacga agtcaacaag ggggattttc actgtgcttt acatcagcgt aggacacaga
    20701 atgttgcttc gtttactgta acgccgggta aatgagcgtt ttttgatagt gcgtaagtgt
    20761 cgtgcgagga aaataagcat ctattattgt ttgtcatgac gacaaggcag gcatcaatga
    20821 aaggcttccc aattgcacat atttttcacc cttcaatccc gccaatgcac gcagtggtta
    20881 acaatcacaa tagaaatatt gattattgga cggtaaaaag aaagtttgca gaaattgtct
    20941 ccaccaatga cgttaataaa atttacagta taagtaatga actccggaga gtattatctg
    21001 caattactgc attgaatttc tatcaaggcg atgttccttc tgtcatgatt ccgaatccaa
    21061 ccggaaaata tgagtccatt catcatagat atttctacag gagaacatga tgattatatc
    21121 atacaaacat tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc
    21181 gtcaataaaa aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc
    21241 acccaaaaag aagccatctt aacaccccct aggcacttta ataaaacctg cattacttca
    21301 gattgttggc aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta
    21361 tttataaagg ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat
    21421 ttagtccgtc gaaaaatgaa tgaccttcct gaaagcccca taggaaataa cctcgaagca
    21481 ctagcaccag gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac
    21541 gcgttacaac caagtggaaa atgcattatc actggtgatt ttcaaaaaga attagcgtac
    21601 ttacaaaatg taattcttta taatgactcg tctcttcgtc tggatttttt aggttataac
    21661 acccaaatta ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa
    21721 aactaggaaa tatctacagg caatatcaat gttaattgcc cattaaaaga tatttacaat
    21781 gaaatcagga ggttgaacgt aatctttagt tgtgggactg gagatatagt tgatctatcc
    21841 tctctggact tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct
    21901 aatactattc taaatcattt taaattggat tcaacaaatt ttactaatgc caacatgttt
    21961 caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa
    22021 ataacgcctg ttttaataag cattagcgat atgtattctg aagaaaaaat caagtttgtc
    22081 gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt
    22141 gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca
    22201 aaagaaatat ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca
    22261 gagatgaaag aattctcatc aaaaaaaaca tatgattaca tcgatacaat cattgaagat
    22321 tatgaaaata tcaaagaaaa tgcttatctg gtcgtccccc aaattaatta tactatggat
    22381 ttaaacatag aagactctag ctcagaagag ttaataccct cgagaaagac gaaaattctc
    22441 cggacaatgg ctttgaggtc ggggaatata acacatatga agcatataac tcagagaatc
    22501 aatattttac cagagaggac tatacgtata attacgacct tttaaatgca atatagactt
    22561 aataacttct tacaaagtct attattaata ccgtagatat tcatcttacc ctgtgcacca
    22621 aaagcaaaaa cttatcgacg cgggtttgaa actgatggca accgcaaaaa tgcctgatgc
    22681 gctacgctta tcaggcctac gccatctctg caatatattg aatttgcgtg cttttgtagg
    22741 caggataagg cgttcacgca gcatccggca tgaataaagc gcactcaaca atctgaaacc
    22801 cgccggaacg gtttattacg tacatcaggt gaaactgacc gataagccgc tttcttttgg
    22861 gtatagtgtc gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa acccgccgaa
    22921 gcgggttttt acgtatatca ggtgaaactg accgataagc cgctttcttt tgggtatagc
    22981 gtcgtggaca gtcattcatc tttctgcccc tccaaaagca aaaacccgcc gaagcgggtt
    23041 tttacgtaaa acaggtgaaa ctgaccgata agccgggttc tgtcgtggac agtcattcat
    23101 ctaggccagc aatcgctcac tggctcaagc agcctacccg ggttcagtac gggccgtacc
    23161 ttatgaaccc ctatttggcc ttgctccggg tggagtttac cgtgccacgg actgttacca
    23221 gccgcgcggt gcgctcttac cgcacccttt cacccttacc tgatcccgct tgcacgggcc
    23281 atcggcggtt tgctctctgt tgcactggtc gtgggtttcc cccccaggcg ttacctggca
    23341 ccctgcccta tggagcccgg actttcctcc cctccacccg tctcccccga agaggacgac
    23401 gacgaagcgg cgactgtctg gtcagcttcg gcgcgcagta tagagggttt gcgcgccctt
    23461 gtcaccccgt gttgcgcatt ccaatcgcca gtgtcgcggc aatattacgt gaagcacggc
    23521 agatattgtc ataagccccg cggaatgctt cgtccaacgt acctatgctg gtcaatacgc
    23581 tgaagaccgc atcaatgcca tgctgatgta caacgccaac atcatcggtc aggctacccg
    23641 caatgccaat caccggttta tggtacttct tcgccacgtt tgcgacgcca atcggtacct
    23701 tgccgtgaat actctggctg tcaatacgcc cttcaccggt gatcaccagc gtacaatcgt
    23761 gaatatgttc ctccagattc agcgccgtag tgacgatttc aataccactt ttcagttccg
    23821 caccaagaaa cgccatcagc gccgcaccca taccgcccgc agctcctgcg ccggggacat
    23881 cttttacatc aacatgcagc gcttttttaa tgacatcggc atagtgagag aggttattgt
    23941 ccagctcaac aatcatcgct tcactggctc ccttttgtgg gccaaagatg cgcgatgcgc
    24001 cgttatcgcc caccagcgga ttggtgacat cacaagcgac gcgaatgacg caatctttta
    24061 agcgcggatc gaggccggaa atatcaatat cattcagagt attaagacta ccgccgccaa
    24121 aaccaatttc attgccgttg gcgtcgcata atttcgcccc cagcgcctgt accatgcctg
    24181 cgccgccatc atttgtagcg ctaccgccaa tgccgataat aatgtttgtc gcgccgctct
    24241 ccagcgcctg caggattaac tcgcctgtgc cgcgtgaagt ggtcacgagt ggatcgcgtt
    24301 tttccgcagg taccagctcc agcccactgg ccgccgccat ttcaataaac gcggttttgc
    24361 catcgccgga gatcccccaa ctggcattca ctttctcgcc cagcggccct gtaacccagg
    24421 cgtgacgttc agaaccctgg gtggctgcaa tcatcgcttc caccgttcct tcgccaccgt
    24481 cggcaaccgg aatagaaacg tactgtgcat caggaaaaat ttcccgaaat cctttttcta
    24541 tcgcctgcgc aacctcgctg gcagataaac tttctttata agagtctggg gcgattacga
    24601 ttttcatacc tatgcctgtt accacatgac gccggagggc gtttctctta ttcggcctgg
    24661 attccaggcc cggattgcaa tacgccatcc gggcacgacg tcattaacga gtaacttcga
    24721 ctttcgccag tttttcgtag tagcacacca gggcgctatg atccgccgtt cctaaaccat
    24781 ctgctcgcag tgcctgcatc atctccataa ccgcagctgt gagcggcagt tgcgcgccga
    24841 cgccgtggga agtatccagc gcatttgcca gatccttaat atgcagatca atacggaagc
    24901 ccggcttgaa gttgcggtcc atcaccatcg gcgctttggc atccagcacg gtactgcccg
    24961 ccagtccacc gcgaattgcc tgatacacca ggtccgggtt aacgcccgct ttagttgcca
    25021 gcgttaacgc ttctgacatc gcggcaatat tcagcgccac aatgacctga ttcgccagtt
    25081 tggtgacgtt acctgcacca attgccccgg tatgcaccac ggaacccgcc attgctttca
    25141 tcaaatcata gtatttgtcg aaaatagcct tgtcgccgcc caccatcact gacagcgtac
    25201 cgtcgatggc tttcggttca ccgccgctca ccggagcatc cagcatatca atgcctttcg
    25261 ctttcagcgc ttcgctgatt tcacggcttg ccagcggtgc gatagaactc atatcgatca
    25321 ataccgtacc tggcttcgcg ccttcaataa tgccattctc acccagcgcc acctctttca
    25381 catgagggga gtttggcagc atggttatga tgacgtcgca ctgttcagcg atcgctttag
    25441 ccgtagaggc tgtttctgca cctgcagcaa tcacgtcagc aatagcttct gggttacggt
    25501 cagcaaccac cagcgagtaa cctgctttca gaaggttttt actcattggt ttacccataa
    25561 tccccaggcc aataaaacca actttcatag tcatatcaat catctctctt gttgcggtgg
    25621 tggttatttt ttaaaggtat cagccagttt atgagtggca gagcggaaga cgccgagatc
    25681 gctgccgaca gccacaaacg tcgcgcccca ttccagataa cgacgcgcat cggcttcgac
    25741 cggcgcgagg ataccgctgg gtttgccgtg cgcgctggca cggttaaaaa tgtgctgaat
    25801 tgctttttgt acatccgggt gtgatgcatt gccgagatgg cctaatgccg cggccagatc
    25861 gctggggccg acgaagatgc cgtctacgcc ttcggtagcg gcaatggcat cgacgttatc
    25921 tacgccctgc tgactttcta tctggaccag aatagtgatg ttcttgttcg actgagcgaa
    25981 ataatccgcc acggtgccaa acatattggc gcggtgagaa acggagacgc cgcgaatgcc
    26041 ttccggtggg taacgggttg atgccaccgc ctgctctgct tcctcttttg tttctacaaa
    26101 aggaatcagg aagttataga aaccgatatc cagaagacgc ttaataattt ccggctcgtt
    26161 ggtcggcact cacactactg gcgcgctggc gctgcctttc aaggccatta actgcggaat
    26221 aaacgtggag atatcgtttg gcgcatgttc gccatccagc accagccagt caaacccagc
    26281 caaaccaaga acttcagtgc taatcgggtt agagagtgct gaccagcaac caatttgtac
    26341 ctgtttcgca gccagtgcgg ctttgaattt attcgggaaa acatcgttat tcatcgctta
    26401 tacctttgct tatttctgca attccatacg tttaatgtcg ccaactacga agaggtagca
    26461 gaccatcgcc atcagcgctg aacatcccac gaaaaccagt gctgcattga aggagtgcag
    26521 ttcacttacc aggtagccaa tcaccagtgg agtgacaatg gaggcaacat tgccaaagac
    26581 gttaaagacg ccgccacaga ggccaacaat ctctttcggc gcggtgtcag aaatcaccgg
    26641 ccagcccaga gcaccaaatc ctttgccaaa aaaagccagc gccatcagca tgaccaccag
    26701 cgtggtgttg ttggtgtagt tacataagat gatggtggaa gccagcaaca ttcccagcac
    26761 aatcggtagc ttacgtgcca gggtcaggga taaaccgcgt ttgatcagat aatccgagaa
    26821 gacacctccc agcacgccgc ccgcaaaacc acacagtgcc ggaatcgagg cgaccagacc
    26881 cactttcaga atcgacatgc ctttttcctg caccagataa atcgggaacc aggtgaggaa
    26941 gaaccaggtg atggtgttga taaaatattg tccgaaaaat acgcccaaca tcatgcggtt
    27001 agagagcaat tgcttgatgt aatgcagttt gggaccgctt gctgccgcgc tgcccggctt
    27061 tttgtggtcc atatcgacca ccgcgccatt ttcagagata aacttcagct cttcctcaga
    27121 catacgtggg tgatctgtcg ggttatgaat caacttgatc cacagcgccg tcagcacaaa
    27181 acaaatcacc cccataacgg taaagacgtg ctcccatccc caggcgaaag tcagccagcc
    27241 aagcagaggc gaaaagagcg ccagcgagaa atattgtgcc gagttaaaga tggcggaggc
    27301 agtaccacgt tctttcgtcg ggaaccaggc ggcgacaata cgggcgttcg ccgggaagga
    27361 tggagcttcc gagaatccca gcataaagcg cataaagaac atggagatcc ctgcccaagc
    27421 cagcgggaac atatcaacaa agccttgcag gaaggtgaac agcgaccaga aaaagaggct
    27481 gtaggtgtaa acttttttcg agccaaactt atcaagcaac cagccgccgg ggatttgcat
    27541 cagcaagtag gcccagccaa aagcggagaa gatgtaaccc atcgaaaccg cacttaactg
    27601 caactctttt gccacttcgg taccagcaat agacagcgtt gcacgatcgg cgtagttaac
    27661 ggcggtaaca ataaaaataa tcagtaatat taaatagcgg gtatgcacac ctttcctttt
    27721 ttcgtcaacg gtgtccagaa tcattttatt tacctcgggt acttatgctg atttttatta
    27781 ttatggggaa ggtgttattt ataagtttca tttatgccgt aacgacaatg aactcgggaa
    27841 ttagtataag cagcgcgaga ataataatca ttgtgcaaat gctaatttaa ttaatacttt
    27901 ttaaatatta ttttgagcat atgcacataa ggttgcgcgc taaagcacag atttgcgctt
    27961 taccttgccg ggcggcactg caatccctaa aatgattgac attgatcaca tttctgcgtt
    28021 taaactcctg acattcttat ttcacccaat gaaatcattt atttttaaat gagaccaggt
    28081 cctcatttta ataacccctg gctggagaat attgcacaat ggccaacatc gaaatcagac
    28141 aagaaacgcc aactgcgttt tatataaaag ttcacgacac agataatgtg gcaattattg
    28201 ttaatgataa tggcctgaaa gcaggaacgc gttttccgga tgggctggaa ttaattgaac
    28261 atattcccca ggggcataaa gtcgcattgc tggacattcc ggctaatggt gaaattattc
    28321 gttatggcga agtgattggt tacgccgtgc gtgcaatccc acgcggaagc tggatcgacg
    28381 aatcaatggt tgtactaccg gaagcgccgc cgttacacac gctgccactg gcaaccaaag
    28441 tcccggaacc cttaccgccg ctggaaggat acacctttga gggctatcgc aatgccgatg
    28501 gcagcgtggg caccaaaaac ctgctcggca tcaccaccag cgtccactgt gtggcaggcg
    28561 tggtggacta tgtagtaaaa atcattgaac gcgatctgct accgaaatac ccgaacgtcg
    28621 atggcgtggt ggggctgaat catttgtacg gttgtggcgt ggcgattaac gcaccggcag
    28681 cagttgtacc tattcgtacc attcacaata tttcgctgaa ccctaacttt ggcggcgaag
    28741 taatggtgat tggcctgggt tgtgaaaagt tgcagcctga gcgcctgctg actggaacgg
    28801 atgatgtgca agctattcca gtagaaagcg ccagcattgt cagtttgcag gatgaaaagc
    28861 atgtcggttt tcagtccatg gtcgaggata ttttgcaggt cgccgaacgc catctacaaa
    28921 aactgaatca acgtcagcga gaaacctgtc cggcttcaga actggttgtc ggcatgcatt
    28981 gcggtggcag cgatgcgttt tctggcgtaa cggcaaaccc ggcggttggc tatgcgtctg
    29041 atctactggt gcgctgcggc gcaacggtga tgttttcaga agtcacggaa gtgcgtgacg
    29101 cgatccatct gctgacacca cgcgcagtga acgaagaggt cggcaaacgg ctgctggagg
    29161 agatggagtg gtacgataac tatctcaata tgggaaaaac cgaccgcagc gccaaccctt
    29221 cgccgggcaa caagaaaggc ggtctagcaa acgtggtaga gaaagcgctc ggctccattg
    29281 ctaaatcggg caaaagtgca attgttgaag tgctgtcgcc cggtcaacgc ccgactaaac
    29341 gcggattaat ttacgccgcg acgccagcca gcgattttgt ctgtggcacg caacaggtgg
    29401 cttcgggtat caccgtgcaa gtgtttacga ccggtcgtgg tacgccgtac ggcctgatgg
    29461 cggtacccgt cattaaaatg gcaccccgca ccgagctggc gaaccgctgg tttgatttaa
    29521 tggatattaa tgcgggcacc atcgctaccg gcgaagaaac tattgaagag gagggctgga
    29581 agttgttcca ctttattctc gacgtcgcca gcgggaagaa gaaaaccttc tcggatcaat
    29641 ggggactgca taaccagctg gcggtgttta acccagcacc ggtgacctga taattcaatt
    29701 cgtattatgc ccgatagcac acgtttatcg ggcatattga gtcacagcga agacgcgtga
    29761 tccccgacca gaatcacttc aaccccagcc tttcgcagtc cttccagact atccgcagga
    29821 atgccttcat caacaatgat catgtcgata cgttgagtat caatgatctt atgtaaactg
    29881 gaacgattga atttactgga atcggtgacc acgatgatcc gttccgcaac ttcgcacatc
    29941 cgacggctta aacgggcttc atcttcatta tgtgtgctga cgccgcgctc cagatcgatc
    30001 gcatctacac caagaaacag catatcgaag tggtaatttt gcagcgattg ctcagcctga
    30061 tcgccgtaaa aagattgcga ctgacggcgc aaatgcccgc cggtcatcag cagctcaacg
    30121 ccttccgctt ccagcaacgc attagccacg ttcataccgt tggtcatcgc aattacgtca
    30181 gtgtacttgc gcatcagacg agcaatctca aaagtggtgg tcccggaatc gaggatcacc
    30241 cgatgacctg gctgaatcaa ctcaacggca gctttcgcaa cgctgcgttt catcgcggtg
    30301 ttcagtgcgc ttttatcttc cactgatggc tcgactgacg gcgtcgtgct atcgcagatc
    30361 aacgcgccac cataggcacg cacagcgatc ccctgctttt ccagaaacgc cagatcattg
    30421 cggatcgtca cagtagatac gccatacaat gccgacagat cgttaacctg cacactccct
    30481 tgctgtcgca gacgctgaat gatctgttct cgtcgctcgc tggtgcctgt cactcgcttc
    30541 tcacctgaag cgtcggtatt actcatagta agtcctttcg taaaactttc gtttcatttc
    30601 gttttgccta ttaacgcctt tctattaagc aaatgcaagc ccaccttgcc cattggcgca
    30661 agctactctc gtttcagtga ctttcattac gtttcttttg tgaatcagat cagaaaacta
    30721 ttatctttcg ttttattttt atctcaccat gacgcagtat caattgaaac aaaacgaaag
    30781 atcaatatcg cagtaatctg aactggagag gaaagtgaaa catctgacag aaatggtgag
    30841 acagcacaaa gcgggcaaaa caaatgcaat ttatgccgtt tgttccgcac atccgctggt
    30901 gctggaggct gcaatccgct acgccagtgc aaaccaaacg ccgttactga ttgaagcaac
    30961 ctccaatcag gtagaccagt tcggcggtta taccggaatg acgcccgccg attttcgcgg
    31021 ctttgtttgt cagctcgccg actcgttgaa tttcccgcag gatgcgctga ttctgggtgg
    31081 tgaccatctg gggccaaacc gctggcaaaa cctgccagcc gctcaggcaa tggccaatgc
    31141 cgatgatttg attaaaagct acgttgcggc aggattcaaa aaaatccacc ttgattgcag
    31201 catgtcctgt caggacgatc caattccctt aactgatgac atcgtggctg aacgcgccgc
    31261 ccgtctggcg aaagtggcgg aagaaacctg tcgcgaacac tttggcgaag ccgatctgga
    31321 gtatgtcatt ggtaccgaag tgccggtacc tggcggcgcg catgaaacct taagcgagct
    31381 ggcggtcacc acgccggatg ccgcccgcgc cacgctggaa gcccatcgtc acgcctttga
    31441 aaagcaaggt ttgaatgcca tctggccacg cattattgcc ctggtggttc aacccggcgt
    31501 cgaattcgat cacaccaacg ttattgatta tcagcccgcc aaagcgagcg ccttaagcca
    31561 gatggtcgaa aactacgaaa cgctgatttt cgaagcgcac tctaccgatt atcaaacgcc
    31621 gcaatctctg cgccagctgg tgattgacca ctttgccatt ctgaaagttg gcccggcgct
    31681 gaccttcgcc ctgcgtgaag cccttttttc tctggcggcg attgaagaag aactggtacc
    31741 agcgaaagcc tgttccggtc tgcgtcaggt gctggaaaac gtgatgctcg accgcccgga
    31801 atactggcaa agccactacc acggtgacgg caacgcgcgt cgtctggcgc gtggttacag
    31861 ctactcagat cgcgtgcgct attactggcc ggacagccag attgatgacg ctttcgctca
    31921 tctggttcgt aatctggcgg attcaccaat tccgctaccg ctcatcagcc agtatctgcc
    31981 gctgcagtac gtgaaagttc gctccggcga gctgcaaccg acgccacggg aactcattat
    32041 aaaccatatt caggacatcc tggcgcagta ccacacagcc tgtgaaggcc aataagcaaa
    32101 acaaagagga acatgctatg ccaaatattg ttttaagccg gattgatgaa cgcttgattc
    32161 acggtcaggt cggcgttcag tgggtcggat ttgcgggggc aaatctggtg ctggtagcca
    32221 acgatgaggt tgccgaagat ccggtacaac aaaacctgat ggaaatggta ctggcagaag
    32281 ggatcgccgt acgtttctgg acgctgcaaa aagttatcga caacattcat cgcgccgccg
    32341 atcgacagaa aatcctgctg gtttgtaaaa cacccgccga tttcctgacg ctggtgaaag
    32401 gtggcgttcc ggtgaatcgc attaacgttg gcaatatgca ctacgccaat ggcaaacaac
    32461 aaatcgccaa aacggtttct gtggatgcgg gcgatatcgc agcatttaac gacctgaaag
    32521 ccgctggggt ggaatgcttc gttcagggcg tcccgacaga gcctgctgtg gacctcttta
    32581 aattactttg agggattcat catggaaatc agcctgttgc aggcatttgc gttgggcatt
    32641 atcgccttta tcgctggcct agatatgttt aacggcttaa cccatatgca ccgcccggtg
    32701 gtcctcggcc cgttggtcgg gctggtactt ggcgatctgc ataccggaat tttaaccggc
    32761 gatacgctgg aactagtgtg gatggggctg gccccgctgg cgggcgcaca gccgcctaac
    32821 gtgattatcg gtactatcgt cggcacggcg tttgccatta ctactggcgt gaaacccgat
    32881 gtcgcggtcg gtgtcgccgt acctttcgct gtcgcagtac agatggggat taccttcctg
    32941 ttctcggtga tgtctggcgt gatgtctcgc tgcgaccgga tggcggagaa cgccgatact
    33001 cgcggcattg aacgcgtgaa ctatctggca ctgctggcac tcggcacttt ctattttctc
    33061 tgcgctttcc tgcctatcta cttcggcgcg gaacatgcaa aaaccatcat tgatgtcctg
    33121 ccgcaacgat taatcgacgg cctcggcgtc gcgggcggca tcatgccagc aatcggtttt
    33181 gccgtgctgc tgaaaatcat gatgaaaaac gtctacatac cctacttcat cctgggcttt
    33241 gttgccgcaa cctggctcaa gttaccggtg ctggctattg ctgccgccgc gctggcgatg
    33301 gcgctgatcg atctgctgcg taaatctcct gaaccgactc aacctgcggc acagaaagag
    33361 gaatttgaag atggcatcta atcaaaccac cctgccgaac gtcagcgaaa atgaagaaac
    33421 actgctgact ggcgtcaatg aaaacgtgta tgaagatcgg agcattgccg cggagctgac
    33481 gaaaaaagat atcaaccgcg tcgcctggcg ttccatgctg ttacaagcgt cttttaacta
    33541 cgaacgtatg caggcttctg gctggttgta cggtctgctg cctgctctga aaaagatcca
    33601 cactaataaa cgcgacctgg cgcgcgccat gaaggggcat atgggtttct tcaataccca
    33661 tccgtttctg gtgacgtttg ttatcggcat tatccttgcg atggagcgtt ctaagcagga
    33721 cgttaacagt attcagagca ccaaaattgc cgtcggtgcg ccgcttggcg ggattggcga
    33781 tgcgatgttc tggctaacgc tactgccgat ttgtggcggg ataggcgcaa gcctcgcgct
    33841 acaaggctct attcttggcg cagtcgtctt tattgtgctg ttcaacgtgg tacacttggg
    33901 gctgcgtttt ggtctggcgc attatgctta ccgcatgggc gtggcggcga ttccccttat
    33961 taaagcgaat accaaaaaag tcggccatgc tgcgtcaatc gttggaatga cggtaatcgg
    34021 cgcactggtg gcaacctatg ttcgtttaag caccacgctg gaaatcacgg cgggcgacgc
    34081 agtagttaag ttacaggctg atgttatcga caaactgatg cctgccttct taccgctggt
    34141 ctacaccctg accatgttct ggctggtacg ccgcggctag agtccgctgc gcctgattgc
    34201 ggttaccgtg gttctaggga tcgtcggtaa attctgccat ttcctttaaa tacaatgagg
    34261 tattagatgt taagtattat tttgacaggg catggcgggt ttgccagtgg catggaaaaa
    34321 gcaatgaagc aaatcctcgg cgatcaatcg cagtttattg ccatcgattt tcccgaaacc
    34381 tcaagcgccg cgctgcttac atcgcagctt gaagaggcca tcgcccaact ggattgtgaa
    34441 gacggcattg tttttctgac ggatttgctg ggcggcacgc cgtttcgcgt agcttcaacg
    34501 ctggcgatgc aaaaaccggg ctgtgaagtg attaccggca ccaatttgca actgctactg
    34561 gagatggtgc tggagcgcga agggttaagc ggtgaagaat tccgtgtaca ggcgctggag
    34621 tgtggacacc gagggctgac cagtctggtg gacgagttag gccgctgcca tgaagaatgt
    34681 ccggtcgagg aaggaatatg acacacgttc tgcgcgccag aaggctgctg actgaagagg
    34741 gatggttcga tgaccatcag ttgcgtgttg ctgacggtgt catcgcagca atcgaaccga
    34801 ttccagtggg cgtgactgaa cgcgatgcgg aacggctctg ccccgcttac atcgacaccc
    34861 atgtacacgg tggtgcgggc gttgatgtaa tggatgacgc gccggatgta ctcgacaagc
    34921 tggcaatgca caaggcacgc gaaggtgtcg gcagttggtt accgaccacc gtaaccgcgc
    34981 cgcttaatac cattcatgcg gcgctgaaac gtattgctca acgttgccaa cgcggcggac
    35041 ctggtgcgca agtgctgggg agttatctcg aaggaccgta cttcacgccg cagaataaag
    35101 gcgcgcatcc g
//
LOCUS       DAJTSF010000033        34996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000033 DAJTSF010000000
VERSION     DAJTSF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.033"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..815)
                     /locus_tag="OO335_001725"
     CDS             complement(<1..815)
                     /locus_tag="OO335_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6002146.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="OO335_001726"
                     /pseudo
     CDS             907..993
                     /locus_tag="OO335_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1398..1874)
                     /locus_tag="OO335_001727"
     CDS             complement(1398..1874)
                     /locus_tag="OO335_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6002147.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="OO335_001728"
     CDS             complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="OO335_001728"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6002148.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3309..4349
                     /gene="bioB"
                     /locus_tag="OO335_001729"
     CDS             3309..4349
                     /gene="bioB"
                     /locus_tag="OO335_001729"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6002149.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4346..5500
                     /gene="bioF"
                     /locus_tag="OO335_001730"
     CDS             4346..5500
                     /gene="bioF"
                     /locus_tag="OO335_001730"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6002150.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5487..6242
                     /gene="bioC"
                     /locus_tag="OO335_001731"
     CDS             5487..6242
                     /gene="bioC"
                     /locus_tag="OO335_001731"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6002151.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6235..6912
                     /gene="bioD"
                     /locus_tag="OO335_001732"
     CDS             6235..6912
                     /gene="bioD"
                     /locus_tag="OO335_001732"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6002152.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7491..9512
                     /gene="uvrB"
                     /locus_tag="OO335_001733"
     CDS             7491..9512
                     /gene="uvrB"
                     /locus_tag="OO335_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6002153.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="OO335_001734"
     CDS             complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="OO335_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6002154.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10877..11021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11009..11998
                     /gene="moaA"
                     /locus_tag="OO335_001735"
     CDS             11009..11998
                     /gene="moaA"
                     /locus_tag="OO335_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6002155.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12020..12532
                     /gene="moaB"
                     /locus_tag="OO335_001736"
     CDS             12020..12532
                     /gene="moaB"
                     /locus_tag="OO335_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6002156.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12535..13020
                     /gene="moaC"
                     /locus_tag="OO335_001737"
     CDS             12535..13020
                     /gene="moaC"
                     /locus_tag="OO335_001737"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6002157.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13013..13258
                     /gene="moaD"
                     /locus_tag="OO335_001738"
     CDS             13013..13258
                     /gene="moaD"
                     /locus_tag="OO335_001738"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6002158.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13260..13712
                     /gene="moaE"
                     /locus_tag="OO335_001739"
     CDS             13260..13712
                     /gene="moaE"
                     /locus_tag="OO335_001739"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6002159.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13849..14553
                     /locus_tag="OO335_001740"
     CDS             13849..14553
                     /locus_tag="OO335_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6002160.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14758..15471
                     /locus_tag="OO335_001741"
     CDS             14758..15471
                     /locus_tag="OO335_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6002161.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15507..16463)
                     /locus_tag="OO335_001742"
     CDS             complement(15507..16463)
                     /locus_tag="OO335_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR6002162.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="OO335_001743"
     CDS             complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="OO335_001743"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6002163.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17701..18462)
                     /locus_tag="OO335_001744"
     CDS             complement(17701..18462)
                     /locus_tag="OO335_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6002164.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18595..19005
                     /locus_tag="OO335_001745"
     CDS             18595..19005
                     /locus_tag="OO335_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6002165.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18967..20073)
                     /locus_tag="OO335_001746"
     CDS             complement(18967..20073)
                     /locus_tag="OO335_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6002166.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20084..21217)
                     /locus_tag="OO335_001747"
     CDS             complement(20084..21217)
                     /locus_tag="OO335_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6002167.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21210..22946)
                     /locus_tag="OO335_001748"
     CDS             complement(21210..22946)
                     /locus_tag="OO335_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6002168.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="OO335_001749"
     CDS             complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="OO335_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6002169.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDEQNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="OO335_001750"
     CDS             complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="OO335_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6002170.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24837..26024
                     /gene="rhlE"
                     /locus_tag="OO335_001751"
                     /pseudo
     CDS             24837..26024
                     /gene="rhlE"
                     /locus_tag="OO335_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="OO335_001752"
     CDS             complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="OO335_001752"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6002171.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26833..28983
                     /gene="dinG"
                     /locus_tag="OO335_001753"
     CDS             26833..28983
                     /gene="dinG"
                     /locus_tag="OO335_001753"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6002172.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29011..29973
                     /gene="ybiB"
                     /locus_tag="OO335_001754"
     CDS             29011..29973
                     /gene="ybiB"
                     /locus_tag="OO335_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6002173.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30114..31199
                     /gene="hcxB"
                     /locus_tag="OO335_001755"
     CDS             30114..31199
                     /gene="hcxB"
                     /locus_tag="OO335_001755"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6002174.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31428..31688)
                     /locus_tag="OO335_001756"
     CDS             complement(31428..31688)
                     /locus_tag="OO335_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6002175.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="OO335_001757"
     CDS             complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="OO335_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6002176.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="OO335_001758"
     CDS             complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="OO335_001758"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6002177.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33012..34996)
                     /locus_tag="OO335_001759"
                     /pseudo
     CDS             complement(33012..34996)
                     /locus_tag="OO335_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8294 a         8971 c         9454 g         8277 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg
     1321 ggaaggtaaa aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc
     1381 gccatctggc agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg
     1441 aaatgaacgt taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct
     1501 acatccagcg cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg
     1561 ccatcgtatc cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct
     1621 accagaccag agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact
     1681 acccagtgcc accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa
     1741 cttttcgttc ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg
     1801 taacccatgc cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta
     1861 ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac
     1921 gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc
     1981 ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt
     2041 gccaaaaggc cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt
     2101 caccggacga gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat
     2161 ttcggcatca cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg
     2221 ctgctgccat tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag
     2281 cggattgccc ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt
     2341 ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc
     2401 tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc
     2461 ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca
     2521 cattttgcgg attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg
     2581 gacaatcggc tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc
     2641 aaaaccaacc atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc
     2701 aaacaagttt tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca
     2761 caccgacatc gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg
     2821 gcgcgcttcg cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc
     2881 tacggaaccg gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag
     2941 tttgcggcat agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat
     3001 ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc
     3061 cgcccaccag gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca
     3121 accttcggcg ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg
     3181 ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa
     3241 attgaaaaga tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga
     3301 gaagccccat ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa
     3361 aaccgttgct ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc
     3421 gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca
     3481 aatactgccc gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag
     3541 ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta
     3601 tgggcgcggc atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc
     3661 agggggtaaa agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc
     3721 aggcgcagcg cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc
     3781 cggagtttta cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg
     3841 aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa
     3901 cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa
     3961 gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg
     4021 ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt
     4081 acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta
     4141 tggcaggcgc aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag
     4201 aagataaaga cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc
     4261 tggcagggga taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg
     4321 acgaatatta caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga
     4381 tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg
     4441 ctggctggtg gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt
     4501 aagccatcat ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg
     4561 tagcggcggc tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga
     4621 actggccgag tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa
     4681 tcaggcagtc attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag
     4741 ccatgcctca ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca
     4801 taacgatgtc gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt
     4861 ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca
     4921 ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat
     4981 cggggagcag gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt
     5041 gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc
     5101 ggattatctg ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca
     5161 ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg
     5221 cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac
     5281 gctggctgat tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt
     5341 acaactggca gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac
     5401 cgtacccgct ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga
     5461 tatcgaccgt ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg
     5521 catttggtcg ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg
     5581 acgccttact ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg
     5641 gacctggctg gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc
     5701 tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag
     5761 gagatatcga atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg
     5821 cggtgcagtg gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc
     5881 ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg
     5941 cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg
     6001 aagagtcgct gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg
     6061 atgatgcgct cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag
     6121 ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc
     6181 aacaacaggg gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt
     6241 aaacgttatt ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca
     6301 cttttacaag ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct
     6361 ggcagcgaaa agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac
     6421 agcagcctgc agctggatta cgcaacggta aatccttaca ccttcgcaga atccacctcg
     6481 ccgcacatca tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga
     6541 ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt
     6601 acgccgcttt ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg
     6661 atactggtag ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca
     6721 atacaacacg ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga
     6781 aaacgtcacg ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga
     6841 gagatcccct ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt
     6901 gccttgttgt agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga
     6961 ctgcaattta accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat
     7021 gcggcgaatg tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact
     7081 attcctgtgg ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg
     7141 cggcttgcac gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact
     7201 ggttagataa atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca
     7261 ctcatcttga caaatgttaa aaaagccgtt gctttgggga taacccggta aggctggagt
     7321 tttatctcgc cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag
     7381 aaatattatg gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac
     7441 gacgagtaaa attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac
     7501 cgttcaaact gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc
     7561 tcgaagaggg gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctccg
     7621 ggaaaacctt caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg
     7681 cgcccaacaa aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa
     7741 acgcggtgga gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga
     7801 gttccgacac cttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt
     7861 tgtccgccac caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg tctgtttccg
     7921 cgatttatgg tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg
     7981 gtatgattat cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta
     8041 atgatcaagc attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc
     8101 cggcagaatc ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat
     8161 tgtcgttatt tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct
     8221 acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca
     8281 aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc
     8341 agcggctgac ccagcgtacc cagtttgatc tggagatgat ggacgagctg ggctactgtt
     8401 cggggattga aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga
     8461 cgctgtttga ttacctgcct gccgatgggc tgctggtcgt cgatgaatct cacgtcacca
     8521 ttccacaaat tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt
     8581 acggcttccg cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag
     8641 cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat
     8701 ccggcggcga tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg
     8761 aagtgcggcc ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg
     8821 caattaacga acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa gatcttaccg
     8881 aatatctcga agaacacggt gagcgcgtgc gttatcttca ctcagatatc gacaccgtcg
     8941 agcgtatgga gattatccgc gacttgcgtc tgggtgagtt cgacgtattg gtagggatca
     9001 acttactgcg tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg
     9061 acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca gaccattggt cgtgcggcac
     9121 gtaacgttaa cggtaaagcg attctctacg gcgataggag cactccatca atggcgaaag
     9181 cgattggcga aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggca
     9241 ttacgccgca aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg
     9301 ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc
     9361 cgatggatat gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa gggctgatga
     9421 tgcaacacgc gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc
     9481 agttgcgtga gttgtttatt gccgcatcgt aacaggatag cgaagaagac tgatgacaaa
     9541 cggaaaacag tctgatgcgc tacgcttatc aggcctacat tttctccgca atatattgaa
     9601 tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca
     9661 ctttgtcttc aataggaagc cggaattttc cttcggattt ccgttaacct aaagcctgta
     9721 acgccttttc cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg
     9781 cctccagtac ctcctggatc acaatccgct ctttcaccgc cgagacatcc acttttggcc
     9841 cgacgatgac cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc
     9901 tttccagctt caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataaa
     9961 ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca
    10021 tcaggctggt ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt
    10081 gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa taactcttga atcggcgtag
    10141 ttaactggtc gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca
    10201 gatcaacagg atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa
    10261 ttaaattgat ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt
    10321 ttccgagatt atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg
    10381 aggcgacgtt cggttccgtt atcagctggt tgaggcagtt gcgcatatcg ccccaagcaa
    10441 tgccgccttc tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga
    10501 tacccgttaa acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc
    10561 ctccgccgag agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc
    10621 ctggagtcag attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg
    10681 ccaatattga ggatacaaac tattttcttc tatctctaaa ggacgatgca cgctatgcct
    10741 ccctgatgat gtatatcaaa gtaaaaccgc cattttccct tattctgtag cgaaatagca
    10801 cgatcatgac gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt
    10861 acacgctagt atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata
    10921 cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac
    10981 ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa ctgactgatg tatttgcgcg
    11041 taagttttac tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg
    11101 cctgccggat ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga
    11161 aattcgccgg gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg
    11221 aggagagccg tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga
    11281 cgctatccgc cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa
    11341 ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc gacagtttgg acgcccgcca
    11401 gtttcacgct attaccgggc aggataaatt caaccaggtc atggcaggga ttgatgctgc
    11461 atttgaggcc ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca
    11521 tcaccagctc gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat
    11581 cgaactgatg gaaacgggcg agggcagcga gctcttccgt aagcatcaca tctctggtca
    11641 ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga
    11701 cggtcccgcg caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc
    11761 gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact
    11821 ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aaggcgatac
    11881 ccagcaacag gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca
    11941 tttcctgcat caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa
    12001 cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc
    12061 tattcttacg gtttctaatc gtcgcggtga agaagacgat acctccggtc actatctgcg
    12121 cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa gccattgtga aagaaaaccg
    12181 ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtatt
    12241 gattacgggt ggtactggcc tgacggaagg tgatcaggct cccgaagcgc tgctgccgtt
    12301 gttcgaccgt gaggttgaag gttttggtga agtgttccgt atgttgtcgt ttgaagagat
    12361 tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa cgctgatttt
    12421 cgccatgccg ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca
    12481 gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg
    12541 caactgaccc atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg
    12601 gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg
    12661 atgattattg atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgccggt
    12721 attcaggcgg caaaacgcac ttgggatctg atcccgctgt gtcacccgct gatgctcagc
    12781 aaagttgaag tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta
    12841 tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg
    12901 gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta
    12961 cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa
    13021 agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc
    13081 ggattaccca accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc
    13141 gctggcgctg gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga
    13201 ccatccgctg actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga
    13261 tggcagaaac caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt
    13321 ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag gtgcgcaacc
    13381 ataacctggg cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa
    13441 aagcactggc agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg
    13501 tgattcaccg catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca
    13561 gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc
    13621 gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg
    13681 agagcgatct gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct
    13741 gcgtccggca atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat
    13801 cattttgcag tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc
    13861 ccacgttctg attcaatcgt acaaccctgg gctggcttgc aaacttatat ggcgcaagtc
    13921 tatggctgga tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat
    13981 tccgcggccg tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc
    14041 gcgcaattag cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta
    14101 acgacgatgc tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc
    14161 attgtctata ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc
    14221 gcaatgagcc tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg
    14281 tttatggcgt taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa
    14341 gcattgatgt gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat
    14401 gacactcaga aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg
    14461 cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa cctgttcctg
    14521 atgttgttac ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc
    14581 atgtgtcggg atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat
    14641 gccttatgag cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg
    14701 tggttttaaa tttattgatg gtgaatatta atgttttttc taaggacaac taaaagaatg
    14761 gaatcatact cgcaaaacag caataaatta gattttcagc acgaggccag gatattaaac
    14821 ggtatatggc tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct
    14881 aagtatctcg aaattacggc aaccaaatat gattcaccac caatgtatgt cgccataggg
    14941 ttattattac tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc
    15001 gcgggcgtaa tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt
    15061 cctgtatatg ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg
    15121 ctggccggtt tattatttaa tgttgatcct ggttctcacc gttttatcat tatgatgacg
    15181 ttgacagggt tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt
    15241 tgggtaataa gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat
    15301 aagctccttg aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc
    15361 gcttttacag gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc
    15421 gcgatagcta taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg
    15481 cagatactaa agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagcttc
    15541 ttcgcttggc tttccagtag caggtaacag atcagcgcca gcagcagcgg gataaagtaa
    15601 tacaggacgc gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca
    15661 gccagtagtg cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg
    15721 acgccagcaa tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga
    15781 ccaagtaaca gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc
    15841 tgagcgaggg cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg
    15901 cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg
    15961 ccgagaatac gcagcgtagt ttgatcgaca taccagtaat ccggcaactc caccacgcct
    16021 gcggtaaaga taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc
    16081 gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc
    16141 ataccaatgc cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac
    16201 accagcatca cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca
    16261 tagcagccgt aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg
    16321 cgattgtagt cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc
    16381 accagcaaca caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc
    16441 cagcgcgggt gtgatttact catcaggggt ttacccccgt gttttcagtt tctacccgat
    16501 cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt ggtgtgtgtg
    16561 ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt
    16621 tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc atccacctgc tgacaatctg
    16681 cggcaataat gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga tcgtggatga
    16741 tgacatttgc ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg
    16801 tcgcccagtg atcgtccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa
    16861 aaacctgaac gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa
    16921 tcggcatatc cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt
    16981 tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatcactt
    17041 cccgctgcgc ctgagtgagc attttcagat aatggcgttc aatatcatcg cgatgttctt
    17101 cgttatcgcg ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt
    17161 tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt tccagctcaa
    17221 actggagaat atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct
    17281 ctggaccgta gctggacata tgctcggcgg agtaattcag cccgccaata aaggcgatac
    17341 gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa
    17401 aaaggcgagg gcggggatcg tagtagcgga acactacgcc agctgccgtc agttcattga
    17461 caaactcatc gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc
    17521 cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt gccgacgtta tcctcaaacc
    17581 agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg
    17641 gataatattg ctcgccgttt tccagcaact ggatcttatt gccttcgcgc cagctacatt
    17701 tcataaatga atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag
    17761 cggcaacgcg gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag
    17821 tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc
    17881 atccagtccg gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc
    17941 acccgccacc aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc
    18001 aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc
    18061 ttttccggtc atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc
    18121 gaccgaaaca tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc
    18181 gtgatgccct tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc
    18241 ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc
    18301 gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcacggcatc
    18361 gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat
    18421 ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc
    18481 tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt
    18541 ccgtaaagtt cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag
    18601 tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta
    18661 ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg
    18721 ggattatgcg cactgtattg tgtcacggta gtgatgatgc tggtgtttca gcgccacccc
    18781 gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt
    18841 ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg
    18901 gcaattgcag ggctggcgat ccttgctggc ccggcggtag tgtctttgct ggcgaaagat
    18961 aagaagttac atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt
    19021 tatcaccagt agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata
    19081 aatctgcttg gtaatgtccg taaagtggcg aatagggtta atccacgtca gattttgcag
    19141 ccacactggc atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat
    19201 aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag cagagtgatg aaatcaacag
    19261 accgaatccc accagcgata aaccgtaaat caccatcgta aagtagaaca gcgctagcga
    19321 tccggcgaag gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa
    19381 ggtggcgaca attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg
    19441 cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt
    19501 gacgatcatt acgccgatag tggtgatcat ggcgatcagc gacggcacca caaaccattt
    19561 gtagtcgaga ttcgggttat accagttgcg taccaccagc tcgctgttgt tgggtttcgg
    19621 ttttccttcc agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc
    19681 aatttgcgca ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa
    19741 ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg ccttttgtgt
    19801 gtcgatggtc gggcggatct cctgtgggct tttcagcagc agcacatgtg taaaggcgct
    19861 ggcgcgggca aaacgttgag taagttccac cgaatgctcg ccgttatctt catcgtagat
    19921 ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac gggaacagga tcacctgaat
    19981 tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg actgcaactc
    20041 tttgcggatt aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt
    20101 tggttttcag ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt
    20161 ttaccaccag caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga
    20221 aataacgagc gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct
    20281 gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag
    20341 cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc agggtactga
    20401 gtaaaaacag gctggagata aaaaacagaa tcagcagcga accgcgatac ggcacgccga
    20461 gaataaacac tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag
    20521 ggatcagctt acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca
    20581 tggtgccgcg ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg
    20641 tcatgataat ggtcaccgca cccgggataa taaagtgctg gcttatcgcc gccgggttaa
    20701 accagtagcg ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc
    20761 gctgcatttg ccagatctgc cagatccctt cgacataccc ctgcacaaag ttagcggtat
    20821 tcggctcact gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct
    20881 gttcggcgaa atccacggga ataaccacca gaccgcgaat tttccccgac tgcattttgg
    20941 cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg
    21001 tgtgggtgaa atccagcgcc gcttcgctac gctgttccag caaaatcccg acccgcagct
    21061 tgctggagtc gaggttaatg ccgtaaccaa aaataaacag cagtagcagt gggatcacta
    21121 ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg
    21181 cccgcacgcg atgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg
    21241 tggatcaact gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagctgac
    21301 tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt tcccacggta caccaggccg
    21361 atgcggtcgc aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct
    21421 ttctctacca tgctgttgat gtgcagccaa aattcacggc gggtgagggg gtcaacgccg
    21481 gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca ggccagcgcc
    21541 agccgctgtt taaaacctaa tggcagttca tcggtggcgt gtgaggcaat acttttcagg
    21601 ccaaatgcct cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac
    21661 acaccagaga aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt
    21721 tgcgccatat agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc
    21781 agcaccagcg cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc
    21841 gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag
    21901 ttgacgtgat cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc
    21961 accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag
    22021 gttccggcac cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg
    22081 atgttgattt ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg
    22141 aggatcagac gtaccgattt cccctgaatc atgccatcgc tgacctgcgg cagtttcaag
    22201 gcgcgttgca acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg
    22261 gccatggttt gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc
    22321 agtaacacgt cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac
    22381 atcccttcgc ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca
    22441 acgccgacgc cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc
    22501 aggccgagtt tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc
    22561 ccaagagacg taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg
    22621 ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca
    22681 aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca
    22741 aagccaatca ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc
    22801 agtgtggttt tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg
    22861 gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg
    22921 ttcagcgtga taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg
    22981 cattccctgg cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag
    23041 gtcggtacgc agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac
    23101 gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt
    23161 gcgccccggc tgggcctggt caagattctg ttcatcaaca taagcgcgca cccacaccgg
    23221 acgcgttagt gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac
    23281 cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag
    23341 ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc
    23401 ctgttcacgg ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc
    23461 ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct
    23521 tttccacagt ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc
    23581 ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag
    23641 atcatactgc gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata
    23701 cggcttgtga tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac
    23761 cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac
    23821 gttgccatac agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc
    23881 aaccacggcg gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac
    23941 aaactccttt gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg
    24001 atcagttcgg tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct
    24061 ttgccaagac ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg
    24121 gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc
    24181 ggactaatca cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg
    24241 gagataaact tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg
    24301 caggcgcgaa ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag
    24361 cgttcggctt cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg
    24421 gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc
    24481 tgcccggcct gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc
    24541 gccagtgcgg cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga
    24601 ttattcatcg cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg
    24661 gagttgtcca gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct
    24721 gcgtcacaaa aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct
    24781 tttcagtatc tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagttatgt
    24841 ctttcgattc tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc
    24901 gtgaacccac ccctattcag cagcaggcga tccctgctgt gctggaaggc cgcgacctga
    24961 tggctagcgc ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac
    25021 acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta
    25081 ccccgacccg tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc
    25141 tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt
    25201 tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc
    25261 aaaatgcagt gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc
    25321 tcgacatggg ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc
    25381 agaacctgct gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt
    25441 tgcacaaccc gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc
    25501 agcacgttca ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta
    25561 aagggaactg gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg
    25621 ctgagcagct caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag
    25681 gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga
    25741 ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aactatgaac
    25801 tgccaaacgt accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta
    25861 ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa
    25921 aactgctgaa aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa
    25981 tcaaagccga accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca
    26041 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg
    26101 aatttgcatg cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca
    26161 agcacttgtc agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc
    26221 caccgagacg ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca
    26281 gtcgattctt gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct
    26341 caaccagttt tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga
    26401 atttagcgcg aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa
    26461 gtggcttgga acgattacgc cccatgcgag cggcgaccat gggggaagaa acccgacgga
    26521 tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg
    26581 gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt
    26641 catcgctggt gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg
    26701 ctcgaacggg cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca
    26761 gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt
    26821 tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag
    26881 gcgcttcagg aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg
    26941 gacgtcgcca aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc
    27001 ggcgttggga aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa
    27061 aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat
    27121 ttaccgctgc tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg
    27181 cgctacgttt gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat
    27241 ctgctggcgt ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt
    27301 gcgaagctga agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat
    27361 atcgctatag atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac
    27421 cgtaactgtt actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa
    27481 gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta
    27541 ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg
    27601 gcccgggatg cgctggagat gagcgctgaa atcaccgtgc cctggtatcg actacagctg
    27661 gacttgttca cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca
    27721 ccgctggcga tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc
    27781 tcgttaaaca acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt
    27841 gcgatgggcg aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc
    27901 gagatgctgc gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc
    27961 cacgatattg tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt
    28021 gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg
    28081 accaaatggg cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg
    28141 ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt
    28201 gtcacctccg caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt
    28261 ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa
    28321 cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa cgaagagcaa
    28381 cacattgccg aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa atatctcggt
    28441 atgttggtac tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat
    28501 ttacgcctga tgttgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac
    28561 cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc atttgccgaa
    28621 gggcttgatt tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg
    28681 cccatcgaca gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat
    28741 ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga
    28801 ctgattcgaa gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgaca
    28861 aaaaattatg gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa
    28921 gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt
    28981 taatgatgtg agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc
    29041 gggcgcggga aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct
    29101 catatgctca atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt
    29161 atcaaagggg aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc
    29221 atcaagctga cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc
    29281 gcgcgtaaac aggctaacct gacgccatta ttggcgattc tgctgcataa actcggtttt
    29341 cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg tgctgacaga aaccattttc
    29401 gaactgatgg gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa
    29461 catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg
    29521 atgcgctggc ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca
    29581 tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc atccggaata cattggacgc
    29641 gtcgcgaagt tctttagcga tatcggcggg tgggcgttac taatgcatgg tactgaaggc
    29701 gaagtgtatg ctaatccgca gcgctgcccg cagatcaatc tcattgaccg tgaagggatg
    29761 cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt tactgccaca agcaaaagat
    29821 ccggaaacca cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc gattcccgaa
    29881 tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc
    29941 gatggcctgg cgcgcgttaa tcaggcattt taatttttat ctcagccgga tgagccacgt
    30001 catccggctt ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccgcgc
    30061 ccggcgtgca gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa
    30121 gtggtcatcg ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga
    30181 tgggtagcga ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg
    30241 cagggcatga ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg
    30301 ggcatctgca aattaaccat catgccaaaa tcgttaaaga ggcgggggcg gcagtcacgc
    30361 tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg ctggggattg
    30421 agaaagcgca tcagcacggt attgctgccg tggcgctaca taactcgcat catatcggcc
    30481 gtatcggtta ctgggcggag cagtgttcag cggcggggtt tgtctctatc cactttgtta
    30541 gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg cgacagccgc tttggcacca
    30601 atcccttctg tgtggttttc cctcgtaaag ataatttccc gctgttgctt gattacgcca
    30661 ccagcgccat tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc
    30721 caggttgcct gattgacgtt aacggcgtgc cgacgaccaa tccggcggta atgcaggagt
    30781 cgccgttggg gtcgctgttg acctttgccg aacataaagg ctacgccctt gcagcgatgt
    30841 gtgaaattct tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa acgttgcaaa
    30901 ccagccccga tgccattctt aactgcatga ccactatcat catcaacccg gaactgttcg
    30961 gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc
    31021 atgatgacga taagccgatt ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac
    31081 ggcaggagca ggggattcca ttggatgcgg gaagctggca ggccatttgt gatgcggcgc
    31141 ggcagattgg tatgtcggaa gagacgttgc aggctttctg tcagcagtta gccagctaaa
    31201 aaaaagcccg tccagtggcg gacgggcaaa caagggtaac ataggatcaa tgagggttag
    31261 agcatatgcg tctgtcggca aacagacagg gaaatacttg tgctggacgt agcgtaaacg
    31321 cctgatccgt ccaacggttc ggattttgta ggcctgataa gacgcggtag cgtcgcatca
    31381 ggcatctgtt gccggatgcg gcataaacgc cttatccgtc ctacgaatta cttataaatt
    31441 accgcagtac cgcttaattt attgttgttg gtggcggaag tgatgctgta tccactggca
    31501 ccggctgccg ccgctttctc agccagtttc gcttccaggg catcgagggt ggatgcgcca
    31561 tcggcagaaa ccacgccgat tttattcatg ttctgtgcct gggatgccgt taccggttcc
    31621 gccgcgaaca cgccaaatga cagagttgaa agagccatag cagcaacaac agtattgata
    31681 gttttcataa ttaatctctc gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt
    31741 gatgagtatc acgttttttt acgagagata aaatcgaaga gaattgaccg ccttgttcaa
    31801 ataaattgat tgatatctaa catactgata aaccatttat ttgttgtact tatggacttg
    31861 ttttatgccg cgtggcagtc acagcagcgt taaaggcacg gaatgccacg caaaatacaa
    31921 attatactcg ctatgcatct cgccagttgt agttaacgta actgtctgtc tttcgaacct
    31981 ctgcgattat atcctgtata cgctggtttt tgtaaatctt tctcctgctg acaatgaata
    32041 cataagcgca cgccaggaat ggcttcccga cgggcttgcg ggatgggggc accgcactct
    32101 tcacattcat acaggctttc gccacgcgga atttcacccc gagcgcgggc aatcgcatct
    32161 tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt tagcccaagc ggatgccata
    32221 tcgtcctccc catatcaatg cttgtacagt taagtgtagc taatataggg acgaacctgg
    32281 gcagttcaag cgtcagatct ccgaccattc ccgcagcaga ttatgataaa gattgagcag
    32341 cgacaggatc tcttcatttt caccatagcg gcttttcagc gactgaatat ttttgtccag
    32401 ttcaaacagc atggcgcgct ttttatcatc gcggatcatc gactggatcc acataaatga
    32461 tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg agggatacaa
    32521 caccagatca cctgccggga gttttacccg atgttgtcca aaggtgtcat taacgaccag
    32581 ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa ggtcagtgcg
    32641 catccagccg ttttgtggat ggctgcgtac tgcgccatcc acatggaaac cataggtttc
    32701 attgttctga tagcgattaa acagcggcgt ggaaagggta cgcggcaagg cagcggcaaa
    32761 gaacaatgca tgttggttaa ccgcgttcag cacctcattt tgcagtgcgg cgtataacgc
    32821 gctgcgggtg tctacttgct gattgttctt aacttgtgca ccttgtgctc cggtggtgac
    32881 gcgtccatcc acccattcgg cttgttccag ttgttcgcga aaatgggcga cgtcctgtgg
    32941 cgataacacg ccgggaatgt ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc
    33001 ccacatctga atcagaaatg catattggct gtgagcaaga aggttcttgg ctcgcccggg
    33061 tgataacggt agccgctctt gttgattgag gcgacgtaat cggtatcaaa caggttgtaa
    33121 acgtttagct ggaagtcgag attgcgatta acccgatacc ccagtttggc atcggcgacc
    33181 cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt ctgaaccttt atgtatactg
    33241 ccgatatagc gtgcgcccgc gccaacagag atatcatcgg ttgcctgata ttggctccat
    33301 aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg aaccatcctg ggcaacatct
    33361 ttgccgtttt tgatggttgc tttttgctgg gtatagccgc caatcacctg ccacgcggga
    33421 gtgatattcc ctgccacgga tatctcatag ccttcgacgc gtttcttacc gtattgcgag
    33481 taagtcccgt catcattttg ctcaacttca ttttcgatat cagtacggaa cagcgcggcg
    33541 gtgagcaaca ggcgtttatc cagaacctgc catttggtgc caatctcgct ggtgttggct
    33601 ttttgcggtt taaaatcggt gcggttggca ctgttaccac tgccagactg cgcaagggcg
    33661 aagttgttgc cgcccggagg ctgctgggaa acggcatagt taatatagac attgccgttt
    33721 tccgtcaggt ggtacagcgc cccggctttc cagttcacca gattgcccga cttggcggtg
    33781 tcgacggtgg tgactggaga accttttgcc acaccagctg ggcaggtgat ggcaccgcgt
    33841 ccgctgccgc cgcaggcggt ggcactgtca tattcagtat gataattatc cagacggatc
    33901 ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta aattgcgaag
    33961 gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg atgaattctg
    34021 ctgtcaggat gataaatatt taccgccggt aacgtcaccg gattaacgcc atagttagtc
    34081 tgcgtttcac gggtaaattc cacgccggta ctgacatcat gaccgataga accggtatag
    34141 aacgtcgagg tcaggttggt ctggttggtg agaattttat tactcacatc tttggtattc
    34201 gccgtgcgtg actaggtcca gctattcaca tcgctggtgg gctgagtaat attcgacgcc
    34261 ccgcccataa tcgccgtcat caggtaatcc tgctttacgc gcgaccaacg ggtagtattg
    34321 cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt gtcggtggtc
    34381 gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag tatcaacttt tccggaatga
    34441 ttcagggccg ccgctcccgc agatggggca gaatagcccg gcaaaccgat ggtcggaatg
    34501 ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat taagatacaa acgatttgct
    34561 gtaccaaggc caaaagcgac agaaggggcg acgccgtaac gctcattttt gactttgtcg
    34621 cgtccggcat cgtgggtttt ttcgcccatc acattcaggc gcaccgcggt agtatcacca
    34681 atgacctgat tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat actggcggag
    34741 gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca tattgatcga gcctgtcggt
    34801 gcgctgcgcc cgtagtcggt gccggacggc cctttaatca cttcgacctg ctcggtattg
    34861 aaggtgtcgc gcgagacgct gccgatatcg cgaataccat caatataaat actgttagag
    34921 gtatcggcac cacgcatata aatggcgtcg ccagtggtgg agttaccgtt ctcacccgca
    34981 aaaaacgcac ccacgc
//
LOCUS       DAJTSF010000034        34903 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000034 DAJTSF010000000
VERSION     DAJTSF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.034"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            858..3143
                     /gene="nrdA"
                     /locus_tag="OO335_001760"
     CDS             858..3143
                     /gene="nrdA"
                     /locus_tag="OO335_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6002178.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3377..4507
                     /gene="nrdB"
                     /locus_tag="OO335_001761"
     CDS             3377..4507
                     /gene="nrdB"
                     /locus_tag="OO335_001761"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6002179.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4507..4761
                     /gene="yfaE"
                     /locus_tag="OO335_001762"
     CDS             4507..4761
                     /gene="yfaE"
                     /locus_tag="OO335_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6002180.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="OO335_001763"
     CDS             complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="OO335_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR6002181.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5644..5886
                     /locus_tag="OO335_001764"
                     /pseudo
     CDS             5644..5886
                     /locus_tag="OO335_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="OO335_001765"
     CDS             complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="OO335_001765"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6002182.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="OO335_001766"
     CDS             complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="OO335_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6002183.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8640..10268
                     /gene="glpA"
                     /locus_tag="OO335_001767"
     CDS             8640..10268
                     /gene="glpA"
                     /locus_tag="OO335_001767"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6002184.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10258..11517
                     /gene="glpB"
                     /locus_tag="OO335_001768"
     CDS             10258..11517
                     /gene="glpB"
                     /locus_tag="OO335_001768"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6002185.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11514..12704
                     /gene="glpC"
                     /locus_tag="OO335_001769"
     CDS             11514..12704
                     /gene="glpC"
                     /locus_tag="OO335_001769"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6002186.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12897..13847
                     /locus_tag="OO335_001770"
     CDS             12897..13847
                     /locus_tag="OO335_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6002187.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13888..14358)
                     /locus_tag="OO335_001771"
     CDS             complement(13888..14358)
                     /locus_tag="OO335_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6002188.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14413..15615)
                     /locus_tag="OO335_001772"
     CDS             complement(14413..15615)
                     /locus_tag="OO335_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR6002189.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15702..16244)
                     /locus_tag="OO335_001773"
     CDS             complement(15702..16244)
                     /locus_tag="OO335_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002190.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16523..16948
                     /gene="nudI"
                     /locus_tag="OO335_001774"
     CDS             16523..16948
                     /gene="nudI"
                     /locus_tag="OO335_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6002191.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16987..17589)
                     /locus_tag="OO335_001775"
     CDS             complement(16987..17589)
                     /locus_tag="OO335_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6002192.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17897..19036
                     /gene="arnB"
                     /locus_tag="OO335_001776"
     CDS             17897..19036
                     /gene="arnB"
                     /locus_tag="OO335_001776"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6002193.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19040..20008
                     /gene="arnC"
                     /locus_tag="OO335_001777"
     CDS             19040..20008
                     /gene="arnC"
                     /locus_tag="OO335_001777"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6002194.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20008..21989
                     /gene="arnA"
                     /locus_tag="OO335_001778"
                     /pseudo
     CDS             20008..21989
                     /gene="arnA"
                     /locus_tag="OO335_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21986..22876
                     /locus_tag="OO335_001779"
     CDS             21986..22876
                     /locus_tag="OO335_001779"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6002195.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22876..24528
                     /gene="arnT"
                     /locus_tag="OO335_001780"
     CDS             22876..24528
                     /gene="arnT"
                     /locus_tag="OO335_001780"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6002196.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24525..24860
                     /gene="arnE"
                     /locus_tag="OO335_001781"
     CDS             24525..24860
                     /gene="arnE"
                     /locus_tag="OO335_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6002197.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24860..25246
                     /gene="arnF"
                     /locus_tag="OO335_001782"
     CDS             24860..25246
                     /gene="arnF"
                     /locus_tag="OO335_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6002198.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="OO335_001783"
     CDS             complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="OO335_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6002199.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25617..26972)
                     /gene="menE"
                     /locus_tag="OO335_001784"
     CDS             complement(25617..26972)
                     /gene="menE"
                     /locus_tag="OO335_001784"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6002200.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26969..27931)
                     /gene="menC"
                     /locus_tag="OO335_001785"
     CDS             complement(26969..27931)
                     /gene="menC"
                     /locus_tag="OO335_001785"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6002201.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27931..28788)
                     /gene="menB"
                     /locus_tag="OO335_001786"
     CDS             complement(27931..28788)
                     /gene="menB"
                     /locus_tag="OO335_001786"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6002202.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28803..29561)
                     /gene="menH"
                     /locus_tag="OO335_001787"
     CDS             complement(28803..29561)
                     /gene="menH"
                     /locus_tag="OO335_001787"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6002203.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29558..31228)
                     /gene="menD"
                     /locus_tag="OO335_001788"
     CDS             complement(29558..31228)
                     /gene="menD"
                     /locus_tag="OO335_001788"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6002204.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31317..32612)
                     /gene="menF"
                     /locus_tag="OO335_001789"
     CDS             complement(31317..32612)
                     /gene="menF"
                     /locus_tag="OO335_001789"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6002205.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="OO335_001790"
     CDS             complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="OO335_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6002206.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33051..33512)
                     /locus_tag="OO335_001791"
     CDS             complement(33051..33512)
                     /locus_tag="OO335_001791"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6002207.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33577..34494
                     /gene="rbn"
                     /locus_tag="OO335_001792"
     CDS             33577..34494
                     /gene="rbn"
                     /locus_tag="OO335_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6002208.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34685..34873
                     /locus_tag="OO335_001793"
                     /pseudo
     CDS             34685..34873
                     /locus_tag="OO335_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8536 a         9341 c         8648 g         8378 t
ORIGIN      
        1 cgtaatgcct gaaaggcttt gcctgctggc gtgacaactc gctttgatat catatccctg
       61 gcataaatca atggccgccg cgacaccgtt agcagagaaa agagatgcaa tcattgacgg
      121 gagtagagat aaatactcct tgcgtagaaa gataatccgc atattaatct gaccatcatt
      181 ttatataaga cggcatattt aaacgcatta tacaatatgg gttatctttc ttttgtaaat
      241 tgtccaaccc gatgcaatgg ctattttgtt attatttgat gaataatatc agtgcgtcat
      301 aattcaagtt aataacctta agggatacca gttatattta aactaaatta aagtaatgaa
      361 taattttctt ataatataag gtaaattaac aaaatggctt cgcatttaac aataaccgaa
      421 tagaaaacaa ccatttcgac atcaacaatc gcttacattc gcttatatat tgaccacaac
      481 tgatacatca gattatgtga tgactcgtgc ttagatcaat ttttgcaatc attagcaaaa
      541 agattaataa accatctata tcaatttatc caacctattc tgccgttcaa gaaatcgccg
      601 aacagttatt tttaacaaat ttttctcttc ccattgactt tcccggacac cttgtctgac
      661 ctaaggtgcg cgaaagccac tttttccttc ctgagttatc cacaaagtta tgcacttgca
      721 agagggtcat tttcacacta tcttgcagtg aatcccaaac atacccccta tatatagtgt
      781 tctaagcagc ttcccttact acaggtagtc tgcatgaaac tattgcggaa agaattccaa
      841 aaacaggtac gacatacatg aatcagaatc tgctggtgac aaagcgcgac ggtagcacag
      901 agcgcatcaa tctcgacaaa atccatcgcg ttctggattg ggcggcagaa ggactgcata
      961 acgtttcgat ttcccaggtc gagctgcgct cccacattca gttttatgac ggtatcaaga
     1021 cctctgacat ccacgaaact attatcaagg ctgccgcaga cctgatctcc cgtgatgcgc
     1081 cggattatca gtatctcgcc gcgcgcctgg cgatcttcca cctgcgtaaa aaagcctacg
     1141 gccagtttga gccgcctgcg ctgtacgacc acgtggtgaa aatggtcgag atgggcaaat
     1201 acgataatca tctgctggaa gactacacgg aagaagagtt caagcagatg gacacgttta
     1261 tcgatcacga ccgtgatatg accttctctt atgctgccgt taagcagctg gaaggcaaat
     1321 atctagtaca gaaccgcgtg accggcgaaa tctacgaaag cgcccagttc ctttatattc
     1381 ttgttgccgc gtgcctgttc tcgaactacc cgcgtgaaac gcgcctgcaa tacgtgaagc
     1441 gtttttatga cgcggtttcc acatttaaaa tttcgctgcc gacgccaatc atgtccggcg
     1501 tgcgtacccc gactcgtcag ttcagctcct gcgtactgat cgagtgcggt gacagcctgg
     1561 attccatcaa cgccacctcc agcgcgattg tgaaatatgt ttcccagcgt gccgggatcg
     1621 gcatcaacgc cggacgtatt cgtgcgctgg gcagcccgat tcgcggtggc gaagcgttcc
     1681 ataccggctg tatcccgttc tacaaacatt tccagacagc ggtgaaatcc tgctctcagg
     1741 gcggtgtgcg cggcggtgcg gcaacgctgt tctacccgat gtggcatctg gaagtggaaa
     1801 gcctgctggt gttgaaaaac aaccgtgggg ttgaaggcaa ccgcgtgcgt catatggact
     1861 acggggtaca aatcaacaaa ctgatgtata cccgcctgct gaaaggtgaa gatatcaccc
     1921 tgttcagccc gtccgacgta ccggggctgt acgacgcgtt cttcgccgat caggaagagt
     1981 ttgaacgcct gtataccaaa tatgagaaag acgacagcat ccgcaagcag cgtgtgaaag
     2041 ccgttgagct gttctcgctg atgatgcagg aacgtgcgtc taccggtcgt atctatattc
     2101 agaacgttga ccactgtaat acccatagtc cgtttgatcc gaccatcgcg ccagttcgcc
     2161 agtctaacct gtgcctagaa attgccctgc cgaccaaacc gctgaacgac gtcaacgacg
     2221 agaacggtga aatcgcgctg tgtacgctgt ctgctttcaa cctgggcgca attaatagcc
     2281 tggatgaact ggaagagctg gcgattctgg cggttcgtgc acttgacgcg ctactggatt
     2341 atcaggatta cccgatcccg gccgccaaac gtggagcgat ggggcgtcgt acgctgggta
     2401 ttggtgtgat taacttcgct tactatctgg cgaagcacgg taaacgctac tccgacggca
     2461 gcgccaacaa cctgacgcat aaaaccttcg aagcgattca gtattacctg ctgaaagcgt
     2521 ctaatgagct ggctaaagag caaggcgcgt gcccgtggtt taacgaaacc acttacgcga
     2581 aagggatcct gccgatcgat acctataaga aagatctgga tgccatcgct aatgagccgc
     2641 tgcattacga ctgggaagca ttgcgtgaat caatcaaaac gcacggcctg cgtaactcca
     2701 cgctttctgc cctgatgccg tccgagacct cttcgcagat ctctaacgcc actaacggta
     2761 ttgaaccgcc gcgcggttac gtcagcatca aagcgtcgaa agacggtatt ttgcgccagg
     2821 tggtgccgga ctacgagcac ctgcacgacg cctatgagct gctgtgggaa atgccgggta
     2881 acgatggcta cctgcaactg gtaggtatca tgcagaaatt tatcgatcag tcgatctctg
     2941 ctaacaccaa ctacgatccg tcacgtttcc cgtcaggaaa agtgccgatg cagcagttgc
     3001 tgaaagacct gctcaccgcc tacaaattcg gggtcaaaac gctgtattat cagaacaccc
     3061 gtgacggcgc tgaagacgcg caggacgatc tggtaccgtc aatccaggac gatggctgcg
     3121 aaagcggcgc atgtaagatc tgatattgag atgccggatg cggcgtaaac gccttatccg
     3181 gcctacggct cggtttgtag gcctgataag acgcgccagc gtcgcatcag gctccgggtg
     3241 ccggatgcag cgtgaacgcc ttatccggcc tacggctcgg atttgtaggc ctgataagac
     3301 gcgccagcgt cgcatcaggc acaggatgcg gcgtaaaatg ccttatccgg cattaaactc
     3361 ccaacaggac acactcatgg catataccac cttttcacag acgaaaaatg atcagctcaa
     3421 agaaccgatg ttctttggtc agccggtcaa cgtggctcgc tacgatcagc aaaaatatga
     3481 catcttcgaa aagctgatcg aaaagcagct ctctttcttc tggcgtccgg aagaagttga
     3541 cgtctcccgc gaccgtatag attaccaggc gctgccggag cacgaaaaac acatctttat
     3601 cagcaacctg aaatatcaga cgctgctgga ttccattcag ggtcgtagcc cgaacgtggc
     3661 gctattgccg cttatttcta ttccggaact ggaaacctgg gtcgaaacct gggcgttctc
     3721 agaaacgatt cattcccgtt cctatactca tatcattcgt aatatcgtta acgatccgtc
     3781 tgttgtgttt gacgatatcg tcaccaacga gcagatccag aaacgtgcgg aagggatctc
     3841 cagctattac gatgaactga tcgaaatgac cagctactgg caccttctgg gcgaaggcac
     3901 ccacaccgtt aacggtaaaa ccgtaaccgt tagcctgcgc gagctgaaga aaaaactgta
     3961 tctctgcctg atgagcgtta acgcgctgga agcgatccgc ttctacgtca gctttgcttg
     4021 ttcctttgcc tttgcagaac gcgaactgat ggaaggtaac gccaagatta ttcgcctgat
     4081 tgcccgcgat gaagccctgc acctgaccgg tactcagcat atgctgaatc tgctgcgcag
     4141 cggcgcggat gatccggaga tggcggaaat tgccgaagaa tgtaagcagg agtgctatga
     4201 cctgtttgtc caggcggcac agcaagagaa agactgggcg gattatctgt tccgtgacgg
     4261 ttcgatgatt ggtctgaata aagatattct ctgccagtac gttgaataca tcaccaatat
     4321 ccgcatgcag gcagtcggtt tggatctgcc gttccagacg cgctccaacc cgatcccgtg
     4381 gatcaacacc tggctggtgt ctgataacgt gcaggttgct ccgcaggaag tggaagtcag
     4441 ttcttatctg gtcgggcaga ttgacgcgga agtggacacc gacgatttga gtaacttcca
     4501 gctctgatgg cccgcgttac cctgcgcatc actggcacac aactgctgtg ccaggatgaa
     4561 cacccttccc ttctggcggc gctggaatcc cacaatgtgg cggttgagta ccagtgccgc
     4621 gaaggttact gcggctcctg tcgcacccga ctggtcgcag gtcaggtgga ctggattgcc
     4681 gaaccgttag cctttattca gccgggggaa attttgccct gttgttgccg ggcaaaaggc
     4741 gatattgaaa tcgagttgtg atcgggtact agcgccagat acgacgctta cgcgtattat
     4801 ctggcctaca acgattacat ggcgtagtaa tacgctttca cctgctccca gtccgcttta
     4861 gggatcggct ccagatattt ttccagctgg cggaagtcat gattaatcgc tttatcgcga
     4921 cgcaagcggc gacgactttt ctccagatca agaaaaccag cttctgcctt accttctgtt
     4981 ttcacataga tatggcgaac atagcaacag ccatgctgac gattaacgct atgcattttc
     5041 ttaaacgcca gtgccactgc tttcaacatg gcttgccgta cttcgtcaga ataaggcgat
     5101 actgcatgcc gggcatacca gtcagcaatg ctgatgaacc ccgccatatc ttcagtcacc
     5161 agcaacgcgc gccattcacc ctcaattttc accgcttcac caaaaacgat cttcggcacg
     5221 atgacaccgg cccgttccag ttctttaatt accgcaacct cacggacaat cgttggtcgg
     5281 ccgaacggat aacgtactga atgaaacaga tgatgcgtca tgcgctttac atacagcttt
     5341 ttgccgttgc gctcgacgca ttgcaccccg ctcataccat tacggcgata gttaggctct
     5401 tcaacccagt cgccctctgt tgcccaccag tgattaaatt cgtcgtactt tgctgaaact
     5461 gccataccct atcgcctgtc atttttatta acgacaatga ctataggtgg ttacctgagg
     5521 aaaatcttaa tgaaacgtgt cgtattaatg aatttctttt gccgctacag gtgtgacacc
     5581 gcttttgccc ttcatttgca gcgcatagat tccaccagcc actaccacta atagtgcgcc
     5641 cagcagataa aatgtgaatt taatcagtcg gatacagcga tgaattttat tcgacaggga
     5701 ttaggcatcg ccttacaacc agagttaacg ctgaaaagca ttgcaggtga attgtgttcc
     5761 gttcctcacg aaccaacctt ctatcgacag atttcgttgc tgactaaaga aaagccggta
     5821 gaaggcagtc cactgttttt actacaaatg tgcatggaac aattagtggc gattggaaaa
     5881 atttgatatg agacaggatg gcgcatgaac gccatcctgc agtaacatta ctctttatta
     5941 agaaatttta ctgccttatc agggaaatca gtaaacagcc catttacacc cgctttgtta
     6001 tacagagcat catataactg attcacatca ggagtgtatt caggcagttt atctgaccgc
     6061 acggtataag gatgcactac cagtttattc tgctgggcat cttgcaccat gccagtgagt
     6121 ttgatattac ccggctgcga tgtctcctca atcaacatat ggtaatccgg accaatacca
     6181 tctgcatatt ccgccacctg tttcatggct cccggcttaa acatccagtc gtagttgtaa
     6241 ttaacccagc ttccatccgg ctgtttctgc tgcgtttcat tccagtcggt ataggcaatc
     6301 agctgtacca gattgagctc catgcccatt ttgggttcca gctcattctt aatacgcttc
     6361 agctcatcag catcaaaaca ttgcaaataa actttatcgt ctttaccggt gtaaccatat
     6421 ttcttcagca cttccagcgt ttttgccgca atatccttcc cttcctgatg atggaaccac
     6481 ggcgctttga tttctggata gataccgata tttttcccgg tagagtgatt taacccctga
     6541 acaaattcaa tctcttcttc aaaggtgtgc acccggaagt cggacttacc cattgggaaa
     6601 cgccccggat aagtctgtac ttttttaccg ttttcaatat cgaaaccttc ggtaaatttc
     6661 agcgacttaa tttcatccag cgtgaaatct atcgcgtagt aacgaccgtc tttgcgcgcc
     6721 cgatccggga aacgatcggc aacatcagta acacgatcga gataatggtc atgcagaaca
     6781 accagatggt cgtctttggt catcaccaaa tcctgttcca gataatccgc tccctgcgca
     6841 tacgccatcg cttttgctgg cagcgtatgc tccggcaaat atccactggc accgcgatgg
     6901 gcgatgacta ttttttcgtt gctgtccgcc gccattgcac tgcttcccat gactatagtg
     6961 ctcatcatga tcgccatgct aaggtttttc agcttcaatt tcatgccatt agcctccgtt
     7021 gcgtttttgc agtaattgtt catggcgacg tttttcgcca atcatcacaa caatcaacaa
     7081 gataaccgcc agaatgctgc cgccaatcat taccataaag ccgccatccc agccgaagaa
     7141 gtccacggtg tagccaacaa tcgcgctcgc cgccaccgaa ccgcccaggt aaccaaacag
     7201 cccggtaaag cccgctgccg tacctgccgc ttttttcggt gccagttcca gcgcatgcag
     7261 accgatcagc atcacaggac cgtagatcag gaagccgata acaatcatac aaatcatatc
     7321 gacggttggg ttacctgccg ggttcatcca gtaaacgata gtcgcgatgg tcaccagtgt
     7381 cataaagaag acgccggttg ccccacggtt accacggaag actttgtccg acatccagcc
     7441 gcacagcagg gtgcccggaa tacccgcata ctcatagaag aagtaggccc aggaggattt
     7501 atccagcgcg aaatgcttaa cctctttcag ataagtcggt gaccagtcga ggatgccgta
     7561 acgcagcaga taaacgaaca cgttggcgat ggcgatgtac cacagcagtt tgttcggcag
     7621 cacgtactgc atgaagattt gcttcgcggt cagctcctgt tccgcttttt cgttatagtc
     7681 gtccggataa tcatttttgt actcttcgat cggcggcaag ccacaggatt gcggggtatc
     7741 gcgcatcatc gcaaaggcga ataatgccac cagaatggcg cagaaagcag gcatatagag
     7801 cgccgcatgc cagtcattga accaggccat ccccagcagg aacagcagcg gcggaatacc
     7861 accaccgacg ttgtgcgcac agttccacac tgacacaatg ccgccacgtt ctttctgcga
     7921 ccaccagtgc accatagtac gaccacacgg cggccacccc atcccctgga accaaccgca
     7981 gaggaacaac agtacaaaca tcaccgcaat gctcgacgtc gcccatggca caaagcccat
     8041 aaacaacatc actgccgccg ccagaatcaa acctgcgggc aggaaaacgc gcggattcga
     8101 gcgatccgat accgaaccca tgatgaattt cgaaaatcca taagcaatcg agatccccga
     8161 aagggcaaaa cctaaatcac cgcgtgagaa tccctgctca accagataag gcatagcaag
     8221 cgcaaagttc ttacgaacca aatagtaagc cgcatagcca aagaatatcc ccaggaaaat
     8281 ttgccagcgc aatcgacgat aagtcggatc gatctccgcg gcaggtaagc gcgctttgtg
     8341 tggcgctggt ttaaaaatac tcaacattga tagcctccgt ggcccgtggt cttatttatg
     8401 attaacagcc tgattcagtg agagaacctg ccgtttcttg agttgccgcg atgttaagaa
     8461 aacattcata aattaaatgt gaattgccgc acacattatt aaataagatt tacaaaatgt
     8521 tcaaaatgac gcatgaaatc acgttttact ttcgaatcat gagcgaatat gcgcgaaatc
     8581 aaacaattca tgtttttact atggctaaat ggtaaaaaac gaacttcaga gggataacaa
     8641 tgaaaactcg cgactcgcaa tcaagtgacg tgattatcat tggcggcggc gcaacgggag
     8701 ccgggattgc ccgcgactgt gccctgcgcg ggctgcgcgt gattttggtt gagcgccacg
     8761 acatcgcaac cggtgccacc gggcgtaacc acggcctgct gcacagcggt gcgcgctatg
     8821 cagtaaccga tgcggaatcg gcccgcgaat gcattagtga aaaccagatc ctgaaacgca
     8881 ttgcacgtca ctgcgttgaa ccaaccaacg gcctgtttat caccctgccg gaagatgacc
     8941 tctcattcca ggccactttt attcgcgcct gcgaagaagc agggatcagc gcagaagcta
     9001 tagacccgca gcaagcgcgc attatcgaac ctgccgttaa cccggcactg attggcgcgg
     9061 tgaaagttcc ggatggcacc gttgatccat ttcgtctgac cgcagcaaac atgctggatg
     9121 ccaaagaaca cggtgccgtt atccttaccg ctcatgaagt cacggggctg attcgtgaag
     9181 gcgcgacggt gtgcggtgtt cgtgtacgta accatctcac cggcgaaact caggcccttc
     9241 atgcacctgt cgtggttaat gccgctggga tctgggggca acacattgcc gaatatgccg
     9301 atctgcgcat tcgcatgttc ccggcgaaag gatcgctgct gatcatggat caccgcatta
     9361 accagcatgt gatcaaccgc tgccgtaaac cttccgacgc cgatattctg gtgcctggcg
     9421 ataccatttc gctgattggt accacctctt tacgtattga ttacaacgag attgacgata
     9481 atcgagtgac ggcagaagag gttgatattc tgctgcgtga aggggaaaaa ctggcccccg
     9541 tgatggcgaa aacgcgcatt ttgcgggcct attctggcgt gcgcccgctg gttgccagcg
     9601 atgacgaccc gagcggacgt aacgtcagcc gtggcatcgt gctgctcgac catgctgaac
     9661 gcgatggtct ggacggattt atcaccatca ccggtggcaa actgatgacc tatcggctga
     9721 tggctgaatg ggctaccgac gcggtatgcc gcaaactggg caacacgcgc ccctgtacga
     9781 ctgccgatct ggcactgcct ggttcacaag aacccgctga agttaccttg cgtaaagtca
     9841 tctccctgcc tgccccgctg cgcggttctg cggtttatcg tcatggcgat cgcacgcctg
     9901 cctggctgag cgaaggccgt ctacaccgta gcctggtatg tgagtgcgaa gcggtaactg
     9961 cgggtgaagt gcagtacgcg gtagaaaatt taaacgttaa tagcctgctg gatttacgcc
    10021 gtcgtacccg tgtggggatg ggcacctgcc agggcgaact ctgcgcctgc cgcgctgccg
    10081 gactgctgca acggtttaac gtcacgacgt ccgcgcaatc tatcgagcaa ctttccacct
    10141 tccttaacga acgctggaaa ggcgtgcaac ccatcgcctg gggagacgca ctgcgcgaaa
    10201 gcgaatttac ccgctgggtt tatcagggac tgtgtggtct ggagaaggag cagaaagatg
    10261 cgctttgata ctgtcattat gggcggcggc ctcgccggat tactctgtgg cctgcaactg
    10321 caaaaacacg gcctgcgctg tgccattgtc acgcgtggtc aaagcgcgtt gcatttctca
    10381 tccggttcgc tggatttgct gagccatctg ccagatggtc aacccgtggc agacattcac
    10441 agtggactgg aatctttgcg tcagcaggca cctgcacatc cttactctct tctcgggcca
    10501 caacgcgtgc tcgatctcgc ttgccaggcg caggcattaa tcgctgaaag cggtgcgcaa
    10561 ttgcagggca gcgtagaact ggctcaccag cggattacgc cgctcggcac tttgcgtgca
    10621 acctggttaa gttcgccaga agttcccgtc tggccgctac ccgcgaagaa gatatgtgta
    10681 gtggggataa gcgggctgat ggatttccag gcgcatcttg cggcggcttc gttgcgcgaa
    10741 ctggatcttt cggttgaaac cgcagaaata gagctgccgg aactggatgt cctgcgcaat
    10801 aacgccaccg aatttcgcgc ggtgaatatc gcccgtttcc ttgataatga agaaaactgg
    10861 ccgctgttac ttgatgcgct tattcctgtc gccaatacct gcgaaatgat cctgatgccc
    10921 gcctgcttcg gcctggccga tgacaaactg tggcgttggt tgaatgaaaa actaccttgt
    10981 tcactgatgc ttttgccaac gctgccgcct tccgtgctgg gcattcgtct gcaaaaccag
    11041 ttacagcgcc agtttgtgcg ccaaggtgga gtgtggatgc cgggcgatga agtgaaaaaa
    11101 gtgacctgta aaaatggcgt agtgaatgaa atctggaccc gcaatcacgc cgatattccg
    11161 ctacgtccac gtttcgcggt tctcgccagc ggcagtttct ttagtggcgg actagtagcg
    11221 gaacgtaacg gcattcgcga gccgattctc ggccttgatg tgctacaaac cgccacgcgg
    11281 ggtgaatggt ataagggcga tttttttgcg ccgcaaccgt ggcagcagtt cggtgtaacc
    11341 actgatgaga cgctacgccc gtcgcaggca gggcaaacca ttgagaacct gtttgccatc
    11401 ggttcggtgc tgggcggatt tgatcccatc gcccagggat gcggcggcgg tgtttgtgcc
    11461 gtcagtgctt tacatgccgc tcaacagatt gcccaacgcg caggaggcca acaatgaacg
    11521 acaccagctt cgaaaactgc attaagtgca ccgtctgcac caccgcctgc ccggtgagcc
    11581 gggtgaatcc cggttatcca gggccaaaac aagccgggcc ggatggcgag cgtctgcgtt
    11641 tgaaagatgg cgcactgtat gacgaggcgc tgaaatattg catcaactgc aaacgttgtg
    11701 aagtcgcctg cccgtccgat gtgaagattg gcgatattat ccagcgcgcg cgggcgaaat
    11761 atgacaccac gcgcccgtcg ctgcgtaatt ttgtgttgag tcataccgac ctgatgggta
    11821 gcgtttccac gccgttcgca ccaatcgtca acaccgctac ctcgctgaaa ccggtgcggc
    11881 agctgcttga tgcggcgtta aaaatcgatc atcgtcgcac gctaccgaaa tactccttcg
    11941 gcacgttccg tcgctggtat cgcagcatag cggctcagca agcacaatat aaagaccagg
    12001 tcgctttctt tcacggctgc ttcgttaact acaaccatcc gcagttaggt aaagatttaa
    12061 ttaaagtgct caacgcaatg ggtaccggtg tacaactgct cagcaaagaa aaatgctgcg
    12121 gcgtaccgct aatcgccaac ggctttaccg ataaagcacg caaacaggca attacgaatg
    12181 tagagtcgat ccgcgaagct gtgggagtaa aaggcattcc ggtgattgcc acctcctcaa
    12241 cctgtacatt tgccctgcgc gacgaatacc cggaagtgct gaatgtcgac aacaaaggct
    12301 tgcgcgatca tatcgaactg gcaacccgct ggctgtggcg caagctggac gaaggcaaaa
    12361 cgttaccgct gaaaccgctg ccgctgaaag tggtttatca cactccgtgc catatggaaa
    12421 aaatgggctg gacgctctac accctggagc tgttgcgtaa catcccgggg cttgagttaa
    12481 cggtgctgga ttcccagtgc tgcggtattg cgggtactta cggtttcaaa aaagagaact
    12541 accccacctc acaagccatc ggcgcaccac tgttccgcca gatagaagaa agcggcgcag
    12601 atctggtggt caccgactgc gaaacctgta aatggcagat tgagatgtcc acaagtcttc
    12661 gctgcgaaca tccgattacg ctactggccc aggcgctggc ttaaactccc ttctgatgcc
    12721 cggtaagcat gtggttaccg ggcatttttg cgtacacgat tccgtgccca atgtatgcgt
    12781 tgcaacgcag tgaaaattcc tctgaaaacg tctcgcaaag gctgaaactg gcagatgtca
    12841 aaggcctggg ataaccgtaa tgtcgcgtca tcataaatat caggtgacgg acaaccatga
    12901 ccgaatcaac aacctcctcc ccgcatgatg cggtatttaa aacctttata ttcacacccg
    12961 aaaccgcccg ggattttctc gaaatacatt taccagaacc actgcgcaag ctttgcaacc
    13021 tgcaaacctt acgcctggaa cccactagtt ttattgaaaa aagtttacgc gcttactact
    13081 cggatgtttt gtggtccgtg gaaaccagcg acggtgacgg ctatatctac tgcgtgattg
    13141 aacatcaaag ctctgcagaa aagaatatgg cttttcggcc aatgcgctat gccactgccg
    13201 ccatgcagag tcacctagat aaaggctatg acagagttcc gctggtggtg ccattgctgt
    13261 tttatcatgg cgaaacatcg ccctacccgt actcacttaa ctggctggat gagtttgacg
    13321 atccgcaact tgcccggcag ttgtacaccg aagcttttcc gttggtggat atcaccatcg
    13381 tacctgacga tgagatcatg caacatcggc gtatagctct gctggaactg attcaaaagc
    13441 atattcgcga ccacgattta atcggcatgg tcgacaggat caccacgctt ttggttagag
    13501 gcttcactaa tgacagccag ctacaaacac tgtttaatta tctgctgcaa tgcggcgata
    13561 cctcccgttt cacccgtttt attcaggaga ttgccgaacg ttcaccacta caaaaggaga
    13621 gattaatgac tattgctgaa cggctacggc aggaaggaca tcaaattggc tggcaagaag
    13681 gtaaattagt aggtttgcag caaggtaaat tagaaggttt gcaggaaggt atgcatgaac
    13741 aagccattaa aattgctttg cgcatgctgg aacagggcat tgatcgtgac caagtgctcg
    13801 cggccaccca gctaagcgaa gccgatctgg cagcgaataa ccactaatta acacaggccc
    13861 acagccgatc cccatgggcc tttgatatca ataactacct tttatgcgtg gcccattttt
    13921 gcctgattta aacatcgcca gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac
    13981 gccaacagcg acaaagttcg ctccccacgc caggcattgc tgcgccatat caggagccac
    14041 agccagaaaa ccagccgctt tacccgcagc acggatccgc cgaatgctgg tttcaataat
    14101 tcgctgcact tccgggtgcc cggcgttatc cgggtagccc aacgacgcag aaagatccgc
    14161 tggtccaata aacacgccat caatcccttc gacgtcgagg atctcgtcca ggttatccag
    14221 tgccgtttta ctttccacct gcactaacag acaaagcgaa tcgttgactt gcgccatgta
    14281 attctcaatg cgtccccagc gcgcagcccg tgccacactg gccccaacac cacgctcacc
    14341 gtagggaggg ttggacaaat tttgccggag ggggaatgtg ccggtgcagt aaaccggcac
    14401 agagtggcga aatcagacgg ataaagtcat ggactcaaca acctcaatcc agccatgctc
    14461 actagcgatg tcctggccgt ttaaccaacg gcgtagcata ttcagtgcca tcattgcgca
    14521 cacttcctga cggatagcca ggctgtagcg tgtagtgctg aaacgcacac gcagagcgaa
    14581 agtgccgtct ggcgtggcta gcgcaaagtt gagatgctcg ttctcgaaac ccgaaacagc
    14641 cagtgccagc ccggcaaaat ggttggcccg ccgttctgta atccagtgcg cagtttgcgc
    14701 cagggtttct tcctgtgaag gaatcacttc acacgccagc aatggagcac ctgcgcgaga
    14761 aagttgcaaa gccaataaac caccggtgaa ttgctcgctc aacgtcaggc tgaactggcg
    14821 gttttgcaat tcgcgactga tctgcgcggg cagtccttcg gtgccttcga aaatcacgct
    14881 ctgtccggca acacgtttaa cgtccagcca cagtttttcc atcgcctgtt gctcgcttgc
    14941 cggtccggtg agtttcagtt cgatgatcgg cattgaggag cgatagccca ttgttacgcc
    15001 cggcggcagt tgtagagtgt ccaggctttg tgccagatcg ctttccgaac gaccaaaggt
    15061 agttaaacgc agacaaaccg gcggctgtgg taaagaaaaa cgctcgcgca ggcgcggcag
    15121 gatttcgtgc tcgaccatca ccttaaattc tgacggtacg ccgggagtaa agaacatcag
    15181 gcaacgatta agctgcacgg caaaaccaca ggcggtgcct accgggttat tgataaattc
    15241 agcactggca ggcagctccg cttgtttacg gttgctcggt gccattactc gtccacgttc
    15301 gtggaaatag cgttccatct ctttgagcca ggcttcatgc agcaccaggc cttcaccttt
    15361 tgctgtcgca gcggcgagtg cgcttaaatc atcgctggtc ggtcccagcc cgccgttaac
    15421 gatcagcaca tcggcgtgct gactacgttc gcgaagaatg gtgactaagt catcaaggtt
    15481 atcccccacc gtattgcggc gagataatgg cagcccctga tgaaagaaaa aatcggccag
    15541 ccaggcagcg ttagtgtcaa cgatttgccc gtataacact tcatccccgg tggatagcat
    15601 ttccactttt aacatggtta tctcctgctt ttgttatcga atcactataa cgcaaaggga
    15661 gataaggcgt agtaagaaac tgaatgccgc gccggaaggg atcagaaact ggcgttaacc
    15721 ccaacataca ggccgtcagc cacagcgttg tcgcggttac cgtctttacc cgacagattc
    15781 aggtagcgat aacccgcctc aatactgact ggacgcataa tggtgtaacg cgcgccagca
    15841 ttcgcttcct cataactttg aataccgctg gagagcgaat ccggagagta gtaatactcg
    15901 ccaaacaaac ggaagctgtt gccaattttc cactgcaaac cacctcctac cgctgcggca
    15961 taaccttcat cgccgtaatt cgggttggtg tacacgcctt ttccgccaac ggtcgccatt
    16021 aacggcccga gaggcagatt caaccccagc cccacgcccg cgacgtcacc gtcgtcgtcg
    16081 ttatgtgtcc agttaccgct taaagccagg cccgtcgatt cagtcccaaa accgacacca
    16141 atattggtgt attctttacc cgcctggccg ctgatgctca ttgcattgac cgatgcagaa
    16201 accaacagca ttccggccag acctgctagc gcaatttttt tcattatccc ctttcccaca
    16261 agcaattaat caaaattccc aaagctgcgg gattgtactg cctggcacag aggattcaat
    16321 gagcgaaaaa agggatctag tcacttattg taactaaata cttcttaaat aattaaacgc
    16381 cgaaaaaata aacacacttt accgatacac acaaaaataa taataagtgc agttatcatt
    16441 gcaaaagaga ttactgtcac tttcacatat ttctgagtta ccgtattctc atggtttctt
    16501 tttatttata aggaataccc ctgtgcgaca acggactatt gtatgccctt tgattcaaaa
    16561 tgatggtgct tatttgctgt gcaaaatggc cgacgatcgc ggcgttttcc ccggtcaatg
    16621 ggcgctttcg ggtggcggcg tggagtctgg cgaacgaatt gaagaggcac tacgccgcga
    16681 aattcgcgaa gaactgggag aacagctgct tttgacagaa atcacgccgt ggaccttcag
    16741 cgatgatatt cgcaccaaga catatgcaga tggtcgcaag gaagagattt atatgattta
    16801 cctgattttt gactgcgttt ctgccaaccg agaagtgaaa ataaacgaag agtttcagga
    16861 ctacgcgtgg gtaaaacctg aagatctggt gcattatgat ttaaatgttg ccacccgaaa
    16921 aacgttacgt ttgaaaggtc ttctttaaca atgatgacag ccatcactgg ctgtcagatt
    16981 gaaaatttag tggttaacga attccccatc cagataaact ttgccttttt ccacatgcat
    17041 gactaaacca tccagataat caggtttaaa tatcgcatca cgcttatttt tagcaatata
    17101 tgtcaggcaa tgattatggg tgaaaataac gatattttta tcaggcgctt tgctttgtaa
    17161 gtccttaatt gcactataaa tctcattacc gcactgaaga agtcgtttat ctaccgtcag
    17221 ctttttaccc gcggaaaacc aggtcgccga ctgaatggtc cggacggtat tactggaata
    17281 aagatcgaaa tcagagatgt cagcgctaaa agcgttgccc agttcacggg catcctgggt
    17341 acctttaacc gtaatacctg ttttatctga caagcattga ttggttgaac gatcgcaacg
    17401 ttcagcatgg cgaaacaaaa cgacaaccgg gtgctgctgt gccagtctgg ccaatgtttt
    17461 attgtcgata cgtggcaaac cattgctact ccaggcggca tgagtaccca gtccggcaat
    17521 tgcagcgagc gccagtaaaa tgatgaaata tttttttgac ttcaatgatg agcggcaaaa
    17581 agctaacacg taaactccac ctatagacaa gcgcaaccag acaattaccg tgaaattgag
    17641 cgacatttct ggcgataatt agcagttggt gtaatattaa aaatcctacg atgtcggcaa
    17701 aatgcctcaa atttttgcca aatgcaaagc ctaaataaga aaaaatataa aaatttcaat
    17761 atttacgtct aatattagtt tcttaaggtt aagttaatat tctatcctta aaatttcgct
    17821 ccaaatggca aaatatacac aacactcttt atagcaaata taagtggaca ggtattcaat
    17881 ggcggaagga aaagcaatgt cagaattttt gcctttttcg cgaccagcaa tgggcgtgga
    17941 ggaactcgct gcagttaaag aggttctcga atccggttgg atcacaaccg gtccgaagaa
    18001 tcaggcgctt gagcaagcct tttgccagtt gacgggaaat cagcatgcca tcgcggtcag
    18061 ttcagccacc gccggaatgc atatcacgct aatggcgttg gaaattggca agggcgatga
    18121 agtgattacg ccttccctga cctgggtttc aaccctcaat atgatttcct tgctgggtgc
    18181 aacgccggta atggtggatg tcgaccgcga tacgctgatg gtcacgcctg aagcgatcga
    18241 atcagccatt acgccacgca ctaaagccat cattccggtg cattatgccg gtgcgccagc
    18301 agatattgac gccattcgcg ccattggcga acgttacggc atcgcagtta tcgaagatgc
    18361 tgcccatgcc gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag gtaccgctat
    18421 tttttcattt catgccatta aaaatattac ctgtgctgaa ggtggcctga ttgtaactga
    18481 taatgaaaac cttgcccgcc agctacggat gctgaaattt cacggtctgg gtgtcgatgc
    18541 ctatgacaga caaacctggg gccgtgcacc gcaggctgaa gtcttaacac cgggctataa
    18601 gtacaatctg accgatatta acgccgcgat tgccctgaca cagttagtca aattagagca
    18661 cctcaacacc cgtcggcgcg aaattgccca gcaatatcag caagcactgg cagctctccc
    18721 ctttcagcca ttaagccttc ccgcctggcc gcacgttcac gcctggcatc tgtttattat
    18781 tcgtgtcgat gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga
    18841 aagaggcatt ggtaccgggt tacatttccg cgccgctcac acacaaaaat attatcgcga
    18901 gcgttttccc acgctgtcgt taccgaatac cgaatggaat agcgaacgca tctgttcgtt
    18961 gccgctgttc ccggatatga ctaccgccga tgccgaccgc gtcatcacag cccttcagca
    19021 actcgcagga caataagcca tgtttgaaat ccaccctgtt aagaaagtct cggtggttat
    19081 tcccgtttat aacgagcagg aaagcttacc ggaattaatc aggcgcacca ccacagcctg
    19141 tgaatcgttg gggaaagagt atgagatcct gctgattgat gacggcagta gcgataattc
    19201 cgcgcatatg ctggtcgaag cctcacaagc ggagaacagc catattgtgt ctattttgat
    19261 taaccgcaat tacgggcaac attcagcgat tatggcggga ttcagtcacg ttactggcga
    19321 cttaattatt acccttgatg ccgatctcca gaatccgcca gaagaaatcc cccgcctggt
    19381 ggcaaaagcc gatgaaggtt acgacgtggt agggactgta cgccagaacc gccaggacag
    19441 ctggtttcgt aaaacagctt cgaagatgat taaccggctt attcagcgca ccactggcaa
    19501 agcgatgggt gactacggtt gtatgctgcg cgcctatcgc cgtcatattg tcgatgcgat
    19561 gttgcactgc catgaacgca gcacctttat cccgattctg gcgaatatct tcgcccgccg
    19621 tgcaattgaa attccagtac atcatgccga gcgtgagttt ggtgaatcca aatacagttt
    19681 tatgcgcctg attaatttga tgtacgacct ggtgacctgc cttaccacaa cgccgctacg
    19741 tatgctgagt ctgctcggca gcattattgc gattggaggt tttagcattg cggtgctgct
    19801 ggtgatttta cgcctgacct tcggaccaca atgggcggca gaaggcgtct ttatgctatt
    19861 tgccgtgctg tttactttta ttggcgctca gtttatcggc atgggattac tcggtgaata
    19921 tatcggcagg atctacaccg atgtccgcgc ccgcccccgc tattttgttc agcaagttat
    19981 ccgtccatcc agcaaggaaa atgaataatg aaaaccgtcg tttttgccta ccacgatatg
    20041 ggatgcctcg gtattgaagc tctgctggct gccggttacg aaattagcgc catttttacc
    20101 cataccgata atcccggtga aaaagccttt tatggttcgg tggctcatct ggcggcggaa
    20161 agagacattc cggtttatgc gccggataac gttaatcatc cgctgtgggt ggaacgcatt
    20221 gcccaactgt caccagaggt gattttctct ttttattatc gccatcttat ttgcgacgag
    20281 atttttcagc tcgctcccgc aggtgcattt aatctgcacg gttcgctgtt accaaaatat
    20341 cgtggtcgcg cgccgctgaa ctgggtgctg gtgaacggtg aaacggaaac gggcgttaca
    20401 ttgcaccgaa tggtaaacgt gccgatgctg gggccattgt ggcgcaactg cgcgttgcca
    20461 ttgcgccaga cgatatcgct attacgctgc atcataaatt gtgccatgcc gcgcgccagc
    20521 tactggaaca gacattaccc gccattaaac acggtaatat tctggaaatc gcccagcgcg
    20581 aaaacgaagc cacctgtttt ggtcgcagaa cgccggatga cagtttcctt gaatggcata
    20641 aaccggcatc cgtactgcac aacatggtac gtgccgttgc cgatccgtgg ccgggtgcct
    20701 tcagctatgt tggcaatcag aaattcaccg tctggtcgtc gcgtgttcat cctcatgcca
    20761 gcaaagcaca gccggggagc gtgatttctg ttgcgccact gctgattgcc tgtggcgatg
    20821 gcgcgctgga aatcgtcacc ggacaggcgg gcgacggcat tactatgcag ggctcgcaat
    20881 tagcgcagat gctgggcctg gtgcaaggtt cacgcttgaa tagccagcct gcctgcaccg
    20941 cccgacgccg tacccgggta ctcatcctcg gggtgaatgg ctttattggc aaccatctga
    21001 cagaacgcct gctgcgcgaa gatcattatg aagtttacgg tctggatatt ggcagcgatg
    21061 cgataagccg ttttctgaat catccacatt ttcactttgt cgaaggcgat atcagtattc
    21121 attccgaatg gattgagtat cacgtcaaaa aatgtgatgt cgtcttgccg ctggtggcga
    21181 tagccacgcc gattgaatat acccgcaacc cgctgcgcgt atttgaactc gattttgaag
    21241 agaatctgcg cattatccgc tactgcgtta agtaccgtaa gcgaatcatc ttcccgtcaa
    21301 cttcagaagt ttatgggatg tgtagcgata aatacttcga tgaggaccat tctaatttaa
    21361 tcgtcggccc ggtgaataaa ccacgctgga tttattcggt gtcaaaacaa ttacttgatc
    21421 gggtgatctg ggcctatggc gaaaaagagg gtttacagtt caccctcttc ctcccgttta
    21481 actggatggg accacgactg gataacctta atgcagcgcg aattggcagc tcccgcgcta
    21541 ttacgcaact cattctcaat ctggtagaag gttcaccaat taagctaatt gatggcggaa
    21601 aacaaaaacg ctgctttact gatatccggg acggtatcga ggcgttgtac cacattatcg
    21661 aaaatgcggg aaatcgctgc gacggcgaaa ttatcaacat tggcaatcct gagaacgaag
    21721 cgagcattga ggaactgggc gagatgctgc tggcgagctt cgaaaaacat ccgctgcgcc
    21781 atcatttccc accgtttgcg ggctttcgcg ttgtcgaaag tagctgctac tacggcaaag
    21841 gatatcagga cgtagagcat cgtaaaccga gcatccgcaa tgcccaccgc tgcctggact
    21901 gggagccgaa aattgatatg caggaaacca tcgacgaaac gctggatttc ttcctgcgca
    21961 ccgttgatct tacggataaa ccatcatgac caaagtaggc ttacgcattg atgtcgatgc
    22021 ctttcgtggc acccgtgaag gcgtgccgcg tctgctggaa atcttgagta agcataatat
    22081 tcaggccagc atttttttca gcgtcggccc ggacaatatg ggccgccatc tctggcgact
    22141 ggtgaagcca cagtttttgt ggaagatgct gcgctcaaac gcggcatcgc tttatggctg
    22201 ggatatttta ctggcaggta cggcctggcc aggtaaagag attggtcatg ccaatgccga
    22261 tatcattcgt gaagcggcta aacatcacga agtcggtctg cacgcctggg atcaccatgc
    22321 ctggcaagcc cgtagcggta actgggatcg gcaaacaatg atcgacgata ttgcacgcgg
    22381 tcttcgcact ctggaagaga ttatcggtca accggtaacc tgttctgccg ctgcgggctg
    22441 gcgtgccgac cagaaggtga tcgaagcaaa agaagcgttc catttgcgct acaacagcga
    22501 ttgtcgtggg gccataccgt tccgtccact gcttgaatca ggaaaccctg gcactgcgca
    22561 aattccggtg accttaccca cctgggatga agtgattggt cgggatgtga aagcagaaga
    22621 ttttaacggt tggttactca accgcatcct gcgagataaa ggcacgccgg tttataccat
    22681 tcatgcagaa gttgaaggct gcgcttatca gcataatttt gtggatctcc tcaaacgcgc
    22741 agctcaggaa ggcgtgacat tttgcccttt aagcgaactg ttatcagaga cgttgccgct
    22801 cggacaagtt gttcgcggaa atattgccgg acgtgaaggc tggctgggtt gccaacaaat
    22861 tgcgggtagt cgctgatgaa atcggtacgt taccttatcg gcatcttcgc gtttattgcc
    22921 tgctattacc tgttaccgat cagcacgcgt ctgctctggc aacccgatga aacgcgttat
    22981 gcggaaatca gtcgagaaat gctggcatcc ggcgactgga ttgtgcccca tctgttaggg
    23041 ctacgttatt tcgaaaaacc cattgccgga tactggatta acagcattgg gcaatggcta
    23101 tttggcgcga ataactttgg tgtgcgggca ggcgttatct ttgcgaccct gttaactgcc
    23161 gcgctggtga cctggtttac tctgcgctta tggcgcgata aacgtctggc tctactcgcc
    23221 acagtaattt atctctcatt gtttattgtc tatgccatcg gcacttatgc cgtgctcgat
    23281 ccgtttattg cattctggct ggtggcggga atgtgcagct tctggctggc aatgcaggca
    23341 cagacgtgga aaggcaaaag cgcaggattt ttactgctgg gaatcacctg cggcatgggg
    23401 gtgatgacca aaggttttct cgcccttgcc gtgccggtat taagcgtgct gccatgggta
    23461 gcgacgcaaa aacgctggaa agatctcttt atttacggct ggctggcggt tatcagttgc
    23521 gtactgacgg ttctcccctg gggactggcg atagcgcagc gggagcctga cttctggcac
    23581 tattttttct gggttgagca tattcaacgc tttgcactag atgatgccca acatagagct
    23641 ccgttctggt actacgtgcc ggtcatcatt gccggtagcc tgccgtggct gggattactc
    23701 cccggtgcac tgtacacagg ctggaaaaac cgcaagcatt ccgcaaccgt ctatttgttg
    23761 agctggacga taatgccgct gctgtttttc tccgtcgcta aaggcaaatt gcccacctat
    23821 attctttctt gctttgcacc tctggcaatg ctgttggcgc attacgcgtt gctggcagca
    23881 aaaaataatc ctctggcgct gcggattaat ggctggatta acatcgcttt tggcgtcact
    23941 ggcattattg ccacatttgt gatctccccc tggggaccaa tgaacacgcc ggtgtggcaa
    24001 accttcgaga gctataaagt cttttgtgcc tggtcgattt tttcgctatg ggcatttttc
    24061 ggctggtaca ccttaacaaa cgtcgaaaag acctggtctt tcgccgcgct ttgcccgctg
    24121 gggctggcgt tgctggtagg attttcaatt cctgacagag tcatggaagg aaaacatccg
    24181 caattttttg tcgagatgac acaagaatca cttcagccaa gccgctatat tcttactgat
    24241 agcgtcggtg ttgccgcagg tctggcatgg agcctgcaac gcgatgacat catcatgtat
    24301 cgccagacag gtgagttgaa atacggcctt aattatccgg atgcgaaagg gagatttgtc
    24361 agcggtgatg agttcgcaaa ctggcttaat caacatcgtc aggaggggat tattacactc
    24421 gtgctttcgg ttgaccgcga tgaagatatc aacagtctcg ccattccgcc cgcagatgcc
    24481 atcgttcgtc aggagcgtct ggtgctgatt cagtatcgtc ccaaatgatc tggctaacat
    24541 tagtctttgc cagcttgctt agcgttgccg ggcagttgtg tcagaaacag gcaacctgct
    24601 ttgtggcgat aaacaaacgg cgcaaacata tcgtgctgtg gctgggactg gcgctggctt
    24661 gtcttggtct tgccatgatg ctctggctgc tggtcttgca gaacgtaccg gtaggcattg
    24721 cctacccgat gttaagtctg aattttgtct gggtgacgct ggctgcagta aaactgtggc
    24781 acgaaccggt atcgccgcgt cactggtgtg gggtggcgtt cattattggt ggcattgtga
    24841 tcctcgggag tacggtgtaa tgtgcctgat atggggatta ttcagcgtca ttattgcctc
    24901 agttgcgcaa ttaagcctgg gttttgcggc aagtcatctg ccgccgatga cgcacttatg
    24961 ggattttatc gcggctctgt tagcttttgg cctggatgcc cggatactgt tactcgggct
    25021 gctgggttat ctgctttccg ttttttgttg gtataaaacg ctacataaac ttgcgctaag
    25081 caaagcctat gccttgctca gtatgagtta tgtgctggta tggattgctt caatggtact
    25141 tcccggctgg gaagggactt tttcgctgaa agcactactg ggagtagcct gtattatgag
    25201 cgggttgatg ctgattttcc tgcccacgac aaaacaacgt tactgagttt tccctgccgc
    25261 tgtacactgc cgttcccact catcggggct ataactgctt gccgagagga ctttcaccgt
    25321 atgcagcttt tcacgattaa tacaataaag cgcattctgc aaaggcgaga gtaaatcccc
    25381 cactttcacg gcgaagtcac tcttcacttc ggcgatcatt tttatcgcgc cgccagcatc
    25441 gcagagcata agtacatgtc tgttatcttg tttattgcag cacgattttt tgtgccagcc
    25501 attccattgc attatcctgt ttgctaagag ttttcacatc aatttacaga ttaacagata
    25561 aactctttat caacggcaat ggaatggcag aaatgagaag tgaaacggag ggttaattat
    25621 tgctgacgtt gcacccactc ttttagcgcc tgacgtgaaa ttttaatacc gccgtttttc
    25681 agctccggcg gtaaagtcag ccagcgcacc ggttgttgaa aacgagccag cttatctttc
    25741 acccattcgc caagatcaac ggtttgctgg tcatactcca ccaccgctac cggccgatga
    25801 ccaaactcct tgtcggcaac ggaaacgata aacacctgca aaaccgcagg atgtgcagca
    25861 ataacacgct cgacttcttc cggctgaata ccctcaccgc cactgaagaa cagattgtct
    25921 aaacgtccga caatggtcag cttgccatta tgcatctcac cgcgatcgcg cgtagcgtac
    25981 cagccttcgt cattaaccag tgaaaccagt tgcccgttac gccagtaacc ttctgccata
    26041 ctggcagccc gcagccacac ttcattatta acgattttca cttcccgacc cggcagcggc
    26101 aaaccaacgt ctgccaggcc gtcggcttct ttcgcacata ccgtggaggc aaactcggtc
    26161 agaccatagc cgcaaaagca acgaatcccc tgctcgcgcg cctgttccgt caactcgacc
    26221 gggatagccg cgccgccaag taacaccgct ttcagggaaa cggaactgcg gttaaccagc
    26281 aaacgccaga gttgtgttgg caccagtgaa gcgtgagtac agcctgccag catttgctcc
    26341 aatggctgtt tatcacgcac cgtcatccgc gcaccagcgt ataaccagcg ccacataatt
    26401 ccctgaccgg agacgtgaaa cagcggtaaa gagagcaacc agtcatcgtg atcgccaaac
    26461 ggaatcagcg ataacacacc ttgcgcactg gcaagatggg cctgataagt atggacagcg
    26521 gctttcggca aaccggtaga acccgaggtc aacgtcattg agcacagacg cgtcggctgc
    26581 cacgcagcgg catgtacgcc ttcaaccagc tgaatgtgca gcgacgttaa tgccggaaac
    26641 gtgttttccc catccggcac cagcgcaaat tgcagcgtca gattgggcag caattcttca
    26701 agcaacggtt gcggcagctg agggttcacg ggcaacaccc gcgccccgca ttgcagtaac
    26761 gccagccagg cgagcagcgt ttgcggcgta ttccacgccc gcaacatcac gccgctgccc
    26821 tcaaccaccc cctgcgccgc aaagcccgta gctaactcat cgacgcgagc acaaagctcg
    26881 cgccagttga gttgctcgtc attaagacgt aaggcgatgg cttctccccg cacttgccgc
    26941 cagtgacgcc acggccagtc agagaagatc ataacaactg ctcctgtaca tcagcgtcca
    27001 ctaacggcag tgggctcccc ggccagcgac gaacctgctg cgcctgcatc agatccagcg
    27061 tgtccagtcc cggaatggtg tctggcgtta accaggcagc aatccgcgcc agttgcgtta
    27121 gacctaagct cgactcaatg gaagaactga tcaccgccgt cagccccagc gcatgcgccg
    27181 cctgtacctg ctcgcgtact ttttccagac tgcccgtgag cgtgggtttg ataactaccg
    27241 cgcgcacgcc ctcttcagcc acaaaggcaa aatccggctc gcgcaggctt tcatcccagg
    27301 caatggcaat gccggtttca cgggcaaacg ctcgcgaatc atcgcgggtt ttgcacggct
    27361 cttcgagaaa cgcgatgcgg tggcgataat ccgggttaac atatttggca aactgctgac
    27421 ctttcagcgg tgtccaggcg cgatttgcgt caagacgcaa atgcagatcc ggaattgcct
    27481 ccagcaacag attcaccacc atgccgtcgc gcaccgcttc gtacaatccc actttgacct
    27541 tcgccacttt ctcgcccggc atatcggcaa gtttgaggat cagatcgtcc ggatcgccat
    27601 tacacagcgg tgccgcacgg tagttggctg cttgcggcaa cgtatctgtc agttctgcca
    27661 atgcacagct tacgccaaag gccacggaag gcatctgcgg tagctcgcaa tcgcctgcca
    27721 gccagttatt tacccaggca agcagcacgc tttgcgcctc ttcccagttt tcctggctga
    27781 agcccggcag tggggagatc tccccccacc cttcgcgctc gccctcacgc aggcaaacgt
    27841 acagcccgtc gcgggttttt aaccgcctgt cgcgcagaac cacccccgcg tccatgggga
    27901 tctgccagcg gtatacctgc gcgctacgca ttacggattc cgtttaaatt tgctgaaatc
    27961 aggctgacgt ttctggttga aagcgttgcg accttcctga ccttcttccg tcatgtagaa
    28021 cagcatggtg gcgttgcccg ccagctcctg cagccccgcc tgcccgtcac agtcggcgtt
    28081 cagcgcagct ttcaggcagc gcagcgccat cgggctgttt tgcagcattt cgcggcacca
    28141 acggacggtt tctttttcca gatccgccag cggtaccacg gtgttgacaa ggcccatatc
    28201 cagcgcctgt tttgcgtcgt actgacggca caggaaccag atttctcgcg cttttttctg
    28261 cccgacgata cgagccatgt aggaagcgcc ccagccgccg tcgaaggaac cgactttcgg
    28321 gccagtctga ccgaagatgg cattatctgc tgcgatagtc aggtcgcaca tcatgtgcag
    28381 aacgtgacca ccgccgatgg agtagccagc caccatcgcg acaaccggtt tcggacaggt
    28441 acggatctga cgctggaagt ccagcacgtt caggtgatgt acgccggaat catctttata
    28501 gccgccgtaa tcaccacgca ctttctggtc accaccggag cagaacgctt tatcgcctgc
    28561 gccagtcaga atgatcacgc cgatattgtc gtcataacgc gcatctgcca gtgcctggat
    28621 catctctttt accgtcagag gacggaaggc attgcgcacc tgcggacgat taatggtgat
    28681 ttttgcgata ccgtcggtgg atttttcata acgaatgtcc tcgaaacctt cggagcagtc
    28741 gtgccattca accggtgcgt aaggcattgc ttcatcagga taaatcatat tgtgtccttt
    28801 agtcagaaac gcaagatctg cgccagactt gcgattacgc cagcgggatt ttcccgatgc
    28861 gcgttatgtc cggcgcgagg aatgacatgg cagtcggcag ccagttccgc cgccagggcg
    28921 cggaatttgc tgtcacgttc accacataaa taataaaacg caaatgtgcg ggcgctaagg
    28981 ttagcacgta aatcaggctg gacggcgaga gaagtcgcct ccagcatggc ggcaagggtt
    29041 gcgccattat tgttgctgcg cagcgccacc agctcccggc gttgatcgtc attgagcgag
    29101 gcaaaaacag gctgttgata ccagtcggca aataccgccg ttaacggttc tgtgcgaaaa
    29161 cgctgcgccc attggcgatc ggaacgctga cgttccgcac gttgttcagc attttgcagc
    29221 cccggatgcc cgccttcgac aataacccca caaagccccg ccagcccctg gcaagccgcc
    29281 atcatcgcca cccgtccgcc aagcgagtac cccaccagcc agaagtcaag gatgttgtaa
    29341 ctaaccaagg ttttacgcag taagtcggtg acatcatcaa atccatcgac gctaatcgcc
    29401 gccgaaccac catgacctag gagatcaaca tacaaccgtg agtagtcggc aaacgcctcg
    29461 cccacttctt gccattcgtg gcaatcgccg gaaaaaccgt gcaaaaacac cagccagggt
    29521 aaacctagtt ttccgtgttt tgcctgcgcg tgcaggatca taaatggctt acctgcgcca
    29581 gaagttgctg gagcgtttgc gcaccatcgg tgtcgttaac caccatttca atcaccgtgg
    29641 tggttggcgt gcgccaggcg tcggcaaatg ccgtttcaag ttcctgccag ttttgcggat
    29701 gatgatattt cagctcgaac atcgcggcgg cgtgctcaaa atggacgttt tgcggcatca
    29761 gatagaaacg ctcacgctcg ctttgcggcg ttggcaacag cgagaaaatt tgcccgccgt
    29821 tgttgttcac cacaattaat accagcggcg cagaaacctg acgcaataac gccagcgcgt
    29881 tgagatcgta aagcgcggag agatcgccca caatcgccag cgtcggtttg ccgcttgccc
    29941 gctgaacgcc tgcggcggtc gaaagcagtc cgtcgatacc gctggcccct cggttgctgt
    30001 acaccgggta acctgccgga agttgcgaaa gcgcatcaat cagacgtacc accaggctgt
    30061 taccaacaaa caattgcccc tgttcaggca gatagtcgct gatgcgatgc gccagttgcg
    30121 cttcgccaaa cgcatcacgg cgggcaataa ccgcctgcat tgcctgttcc gccaggcgcg
    30181 ggatttcaac gcaccagggc tggcgttttt ctgccggatg cagctccagc cagtcggcaa
    30241 tattggcaat taagcgacgt ccgcgatggt gtgccggatc aagtcgccct tcaatgtcat
    30301 caacaatcca gtactcttct ggttcacagc ttgcctgcca ttgcaggagc cgtttccccg
    30361 tcaggctact tcccagttgc accacaattt gcgcctgctg cagctcgctg gtcgctttgg
    30421 cattgcctaa ccaaagatcg gcacacggca gcggctgccc ggtttgtgac agcacatcgc
    30481 caatcagcgg ccagccaaga gtttgcgccc acagggcaac ttttttgccc tcttccgcac
    30541 tcatgcgccc ggcaaccacc acgccgcgct tttgtcgcca gaagaaccag tcgcgctgtt
    30601 tttcactttc cagacgaggc gcttcacgca gccacggttt gtcgtcctgc caccagtcac
    30661 ccagacgctg ttgccagcta agcccggtat cgtccatttc gccatacagc ggttcagcaa
    30721 acgggcagtt gatatggact ccccccgcat gaagcgtacc gagagcgtgg tcgatggtag
    30781 aaaccagcca acgtgcgggg atatcctggg tcgggcgcgg caatgaaata ctgtgcgtgg
    30841 ggtgagaggc gaacattccc ggctggcgaa ttgcctgatt cgcgccgcag tcaattagct
    30901 ccggcgggcg atcggcggtt aagagaatca gtttttctcc ggttaacccg gcttcaatca
    30961 gtgccggata gagatttgcc accgccgtgc cggaggtcac aatcaccgcc accggctgct
    31021 tgctgacttt cgccagcccc agcgccagat gccccaaccc acgctcatcg aaatgggtgt
    31081 ggtgaatgaa tgcggaattc tccgccgccg ctaacgttaa cggtgtagaa cgcgagcctg
    31141 gggcgataca gatgtgtctg acgccgtgac gcgttaatgc ttccagaatg accgccgccc
    31201 agcgtcggtt aaatgcgctt actgacatga gtttgtccgg tatcaatatt gcggctaagt
    31261 ataaggagct acaaaaatag attattgata tgaatcggta atgatgcgac tcattactat
    31321 tccatttgta ataaagtacg cagccctgcc gctttgttgt cgatttcctg ccactcttgc
    31381 tcggggtcgg aaccacggac aatgcccgcg ccagcatata atcgcacgac attgccgcta
    31441 atttttgctg agcgcaggga aacgcagaat tcgctttgtt gtaatgagag atagcccgca
    31501 gaaccggcgt accattctcg ggtgaacggt tcgtgacggg cgataaactg tcgcgccaga
    31561 tcgcgcggta agccagcaac tgctgccgtt ggcagcaact gatgtaaaca gatcacatca
    31621 tccgctttgt tgagtgaagt ccagatacag cggcgaagat gctgcacttt acgcagacgc
    31681 agtacctgcg gcggtaaaac atccagcgtc tgggtatcgg cctgtaatcg ttggcagata
    31741 tcttccacca ccagcatgtt ctcgcgctgg tttttatcat ccgccatcag ccactctcct
    31801 aactgctgcg cctgcttatc atcaggatga tttgctactg ttcccgccag cgcttcagta
    31861 cgcagcgctt tgtcacgccg ccgccataac cgttccggtg aagagccaag aaaagcattt
    31921 tcgccatcaa aggccatgta aaaatggtag caattcagat tcagtcgacg actggcagcc
    31981 atcatcgccg ccgcgttgac cggacttgcg aaatgcaggt cagttgcccg agcgagcacc
    32041 actttgtcga gctcaccttc ggcgatggtt ttcgttgcca gttcgattaa ttgcgtccag
    32101 cccgttttgt ccggccagtg ttgttctcgc gtggtggtta aatgtaaccc aggcaagggc
    32161 ttgatactca ccagtgtggc gataaattct tttgcctgaa tcgcatcgtg ctgaagggag
    32221 ctttcgctga atagcgtcag ccgcagcgtg gctttaccgc cacagcgtcg ccattccagg
    32281 cggggtaaaa gtaaattgcc ctgcgacggg tcaaaagcat tcagccccca aatgcgtaag
    32341 tcagcgtgtt ccgggtgctg gcgaagaaaa cgttgtgcct ggtccaacga cgtaaaacgg
    32401 gtaatcgcgc ccaggacggc agcttcttca tcaccattac gttgttgcca gtagaattgc
    32461 gggtacgttt gctgactggc cagccagctc aaggcatcaa aagcgtcttt gagagggaaa
    32521 ggaatatcga taacccgaat gccgggtgtc gccggaatct cttgcgacaa atggcgcagt
    32581 agattttcca gcgccgtagt aagtgattgc acgcggacct ctccctgtta aaagcctcac
    32641 attatacggg gtactacaaa aaaaatgcag taccccggtg tagggaggtt ttaacggcgt
    32701 gccagcaaca gtcctagtac cagcccgacg gccgcgccca caccaattcc ttgccagggt
    32761 ttttcgtgga cgtagtcatc agcacgataa acagcctgct tcgcccgata gtaataactg
    32821 tctgaagcct ggctaacccg ttttttcaca tcatccagcg ctttttctgc atgcgctttc
    32881 agctcaacat atttctgatc ggcgggatcg ccagaggagc ggagcacctc ttccagtgtt
    32941 tcactaagca gcgtcaggtc gtcatcgata cgtgtatcac caaactgatt agacattctc
    33001 gttctccatt tgcgtaaaac ctgtcactaa ctatagacaa gagttgttga ttacgcctga
    33061 attacctcgc gtgccatccc aatgtgtggg ataccatctt cttcatagac ctccgtcaca
    33121 gggataaaac cgaaactctg gtagaagttt tgcagatgcg cctgcgcccc gaggtaaaca
    33181 ggcttatcag gccagtgatg cgtacatgtt tcgagtgttt tactcatcag ctgctgcccc
    33241 actttttcac cacgcaacgc ttcactgaca atcacccgac ctataacgac cggctcaaga
    33301 tcatcatcac ttttcagaat cctcgcatac gccaccagtt catcattttt ccaaccgaga
    33361 atatggcgat tatcccccgt caaatcatca ccatcgatat cctgataagg gcaattctgc
    33421 tcgacaacaa acaccgcgca acgcagttgt aataaggcat aaagctggga aaccgataat
    33481 tcagagtgat gcagatcttg ccattcaatc ataatgctct ccttttagat gtatgcctta
    33541 tactagccgg aatatttttt gaaacgtgat gaactcatgg aattaatttt tttaggtact
    33601 tcagccggtg tgccaacccg cacgcgcaat gtcacggcaa tattgcttaa cctgcaacat
    33661 ccgacccaga gcggactttg gttgtttgac tgcggtgaag gcacccagca tcagctactg
    33721 cataccgcct ttaaccctgg aaaactggac aagattttta tcagtcacct tcatggcgat
    33781 catctttttg gtttacccgg cttgctgtgc agtcgttcta tgtcaggcat tatccaaccc
    33841 ttaacgattt atggtccgca aggtatccgt gaatttgtgg aaaccgcgct gcggattagc
    33901 ggctcatgga ccgattatcc gctggaaatt gtcgaaattg gcgctggcga aattctcgat
    33961 gatggcctgc gcaaagtaac cgcttatccg ctggaacacc cactggaatg ttatggctat
    34021 cgtattgaag aacatgataa accgggtgca ttaaatgccc aggcattaaa agctgctggc
    34081 gtgccgcctg gcccactgtt tcaggaatta aaagcgggca aaacaatcac gctggaagat
    34141 ggaaggcaga ttaacggcgc agattactta gctgctccag tgccaggtaa agcgctcgct
    34201 attttcggcg ataccggccc ctgcgatgcc gcacttgacc tggctaaagg tgtcgatgtc
    34261 atggtgcacg aagcgacgct ggatataacc atggaagcca aagccaatag tcgcggccat
    34321 agctctacac gccaggctgc gacactagcc cgtgaggctg gagtcggcaa gctaatcatt
    34381 acccacgtca gctcgcgcta tgatgacaaa ggttgtcaac acctgttacg tgaatgcagg
    34441 tcaattttcc cggcgactga actggcgaat gatttcaccg tgtttaacgt ttaactctgc
    34501 ttattattaa aaacagggtg aaacttgccc tgttatagca acccgcgcta catatatcca
    34561 cccacacagg catattgagc gatttttgat atttattcat ctccttacgc actatattca
    34621 gagcaataag ttgcctcttt tcatcttgat tcagcacaac ctttgcaact aaaaggagcc
    34681 catgatggtt acagttgtca gcaattattg tcaattatct caaacccaac tcagtcagac
    34741 atttgcagaa aaatttactg tgaccgagga attactgcag tctttaaaaa aaacagcgtt
    34801 atccggagat gaagaaagta ttgagttact gcataatatt gcgttaggtt atgatgaatt
    34861 tggaaaaaaa gctgggtaat gctgccaact tactgattta gtg
//
LOCUS       DAJTSF010000035        34801 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000035 DAJTSF010000000
VERSION     DAJTSF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.035"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2098
                     /locus_tag="OO335_001794"
     CDS             <1..2098
                     /locus_tag="OO335_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR6002209.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="OO335_001795"
     CDS             2095..3183
                     /locus_tag="OO335_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6002210.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO335_001796"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO335_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR6002211.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="OO335_001797"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="OO335_001797"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6002212.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="OO335_001798"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="OO335_001798"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR6002213.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="OO335_001799"
     CDS             8051..8602
                     /locus_tag="OO335_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6002214.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="OO335_001800"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="OO335_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6002215.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10447..11412)
                     /gene="alx"
                     /locus_tag="OO335_001801"
     CDS             complement(10447..11412)
                     /gene="alx"
                     /locus_tag="OO335_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6002216.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11683..12681)
                     /locus_tag="OO335_001802"
     CDS             complement(11683..12681)
                     /locus_tag="OO335_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6002217.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12760..13452)
                     /locus_tag="OO335_001803"
     CDS             complement(12760..13452)
                     /locus_tag="OO335_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6002218.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13529..14032)
                     /locus_tag="OO335_001804"
     CDS             complement(13529..14032)
                     /locus_tag="OO335_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6002219.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14117..15253
                     /gene="rlmG"
                     /locus_tag="OO335_001805"
     CDS             14117..15253
                     /gene="rlmG"
                     /locus_tag="OO335_001805"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6002220.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15538..15852
                     /gene="higB"
                     /locus_tag="OO335_001806"
     CDS             15538..15852
                     /gene="higB"
                     /locus_tag="OO335_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6002221.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15849..16265
                     /gene="higA"
                     /locus_tag="OO335_001807"
     CDS             15849..16265
                     /gene="higA"
                     /locus_tag="OO335_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6002222.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="OO335_001808"
     CDS             complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="OO335_001808"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6002223.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="OO335_001809"
     CDS             complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="OO335_001809"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR6002224.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20922..21341)
                     /locus_tag="OO335_001810"
     CDS             complement(20922..21341)
                     /locus_tag="OO335_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002225.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21319..21672)
                     /locus_tag="OO335_001811"
     CDS             complement(21319..21672)
                     /locus_tag="OO335_001811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002226.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21673..21993)
                     /locus_tag="OO335_001812"
     CDS             complement(21673..21993)
                     /locus_tag="OO335_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002227.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22127..23560)
                     /locus_tag="OO335_001813"
     CDS             complement(22127..23560)
                     /locus_tag="OO335_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6002228.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23623..24072)
                     /locus_tag="OO335_001814"
     CDS             complement(23623..24072)
                     /locus_tag="OO335_001814"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6002229.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="OO335_001815"
     CDS             complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="OO335_001815"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR6002230.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="OO335_001816"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="OO335_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6002231.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="OO335_001817"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="OO335_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="OO335_001818"
     CDS             complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="OO335_001818"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6002232.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30709..32229
                     /gene="aer"
                     /locus_tag="OO335_001819"
                     /pseudo
     CDS             30709..32229
                     /gene="aer"
                     /locus_tag="OO335_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="OO335_001820"
                     /gene_synonym="yqjI"
     CDS             complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="OO335_001820"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6002233.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33283..34028
                     /gene="nfeF"
                     /locus_tag="OO335_001821"
                     /pseudo
     CDS             33283..34028
                     /gene="nfeF"
                     /locus_tag="OO335_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34082..34157)
                     /locus_tag="OO335_001822"
     tRNA            complement(34082..34157)
                     /locus_tag="OO335_001822"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34122..34124),aa:Ile,seq:cat)
     gene            34282..34788
                     /gene="mug"
                     /locus_tag="OO335_001823"
                     /pseudo
     CDS             34282..34788
                     /gene="mug"
                     /locus_tag="OO335_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8488 a         8949 c         9145 g         8219 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggtttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacca tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    10201 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10261 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10321 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10381 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10441 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10501 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10561 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10621 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10681 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10741 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10801 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10861 ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10921 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10981 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    11041 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    11101 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    11161 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    11221 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11281 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11341 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11401 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11461 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11521 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11581 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11641 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11701 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11761 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11821 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11881 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11941 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    12001 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    12061 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    12121 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    12181 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12241 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12301 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12361 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12421 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12481 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12541 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12601 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12661 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12721 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12781 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12841 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12901 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12961 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    13021 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    13081 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    13141 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    13201 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13261 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13321 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13381 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13441 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13501 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13561 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13621 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13681 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13741 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13801 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13861 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13921 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13981 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    14041 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    14101 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    14161 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    14221 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14281 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14341 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14401 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14461 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14521 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14581 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14641 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14701 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14761 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14821 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14881 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14941 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15001 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    15061 cgataacgtc gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    15121 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    15181 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15241 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15301 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15361 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15421 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15481 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15541 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15601 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15661 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15721 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15781 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15841 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15901 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15961 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    16021 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    16081 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    16141 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    16201 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16261 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16321 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16381 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16441 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16501 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16561 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16621 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16681 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16741 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16801 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16861 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16921 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16981 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    17041 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    17101 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    17161 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    17221 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17281 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17341 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17401 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17461 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17521 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17581 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17641 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17701 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17761 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17821 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17881 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17941 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    18001 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    18061 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    18121 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    18181 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18241 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18301 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18361 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18421 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18481 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18541 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18601 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18661 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18721 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18781 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18841 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18901 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18961 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    19021 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    19081 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    19141 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    19201 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19261 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19321 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19381 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19441 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19501 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19561 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19621 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19681 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19741 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19801 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19861 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19921 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19981 ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac cctttcttca
    20041 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    20101 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    20161 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    20221 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20281 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20341 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20401 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20461 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20521 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20581 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20641 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20701 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20761 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20821 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20881 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20941 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    21001 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    21061 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    21121 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    21181 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21241 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21301 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21361 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21421 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21481 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21541 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21601 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21661 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21721 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21781 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21841 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21901 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21961 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    22021 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    22081 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    22141 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    22201 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22261 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22321 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22381 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22441 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22501 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22561 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22621 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22681 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22741 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22801 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22861 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22921 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22981 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    23041 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    23101 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    23161 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    23221 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23281 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23341 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23401 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23461 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23521 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23581 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23641 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23701 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23761 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23821 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23881 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23941 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    24001 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    24061 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    24121 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    24181 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24241 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24301 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24361 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24421 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24481 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24541 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24601 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24661 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24721 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24781 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24841 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24901 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24961 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    25021 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    25081 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    25141 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    25201 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25261 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25321 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25381 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25441 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25501 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25561 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25621 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25681 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25741 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25801 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25861 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25921 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25981 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    26041 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    26101 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    26161 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    26221 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26281 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26341 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26401 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26461 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26521 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26581 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26641 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26701 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26761 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26821 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26881 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26941 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    27001 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    27061 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    27121 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    27181 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32281 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32341 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32401 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32461 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32521 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32581 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32641 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32701 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32761 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32821 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32881 acattgaccg tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc
    32941 tttgcagcag ccctcatggc gtggctggcc ttcatgttta caacaccctt cgtgatgatg
    33001 gctcatatca gcctcctttt gcttgattag atatatctaa tttatatctg atgtgagcat
    33061 tactgcaagt acaaaaatga tttttaaacg tggaagcacc tcacatctag gccggataag
    33121 acgttcacgc cgcatccggc agcattgcta tggcactgaa tgccttcaca tacttcagac
    33181 atatcagttc tacaaatcgc ttgcatttat catgattaac aatcattatc atttgcgaat
    33241 tttatttaga tatatctgat taccatcacg aaggcgataa caatgaataa caccccccgc
    33301 tacccgcagc gcgttcgcaa tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag
    33361 cgcatcagcg ccggttttca gcgcattgtc cttggcggcg aggcgttgga cggttttaca
    33421 tcgcgcggct ttgacgatca cagcaaactc ttctttcctc aacctgacgc tcactttgtg
    33481 ccgccaacgg taacggaaga gagcatcgtc tggccggaag gaccacgccc accgtcgcgt
    33541 gactatacgc cactgtatga cgaactacgc catgaactgg cgatcgattt ctttattcac
    33601 gacggtgggg tcgccagcgg ctgggcgata aacttacggt ggcaggtccg cgcggttcgc
    33661 tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg
    33721 cactgcgccg ccgcctggaa acgttgagca aacttgccgt taaaccgcaa gttagtgcgc
    33781 tggttagcgt gcgggataac gcctgtcagg attatctcgc gcaccttgat ggttttaata
    33841 tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc
    33901 ctgccgatga ttacttcatc tggataaccg gcgaaggtaa agtcgttaag aatttaagcc
    33961 gccgctttga agcggaacag tatgacccac agcgggtacg tgcagcggct tactggcacg
    34021 caaagtaact gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat
    34081 ctggtggccc ctgctggact tgaaccagcg accaagcgat tatgagtcgc ctgctctaac
    34141 cactgagcta aggggccatg gtagcggatt ataaagtaac tccgtgccgc aatccagcca
    34201 ttaccgcgcg cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg
    34261 tgatgttaga aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg
    34321 ttttgcggta tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatccg
    34381 gcaaatcgct tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg
    34441 caggaggcac agcatctgct ggattatcgt tgtggcatca ccaaactggt agaccgtcca
    34501 acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg taagctgatt
    34561 gaaaaaattg aagattatca gccgcaggcg ttggctattc tgggcaaaca agcatatgaa
    34621 cagggattca gctagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg
    34681 cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg tttcactgga gaaactggtt
    34741 gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg ggcgataaaa aacgtcaccg
    34801 g
//
LOCUS       DAJTSF010000036        34444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000036 DAJTSF010000000
VERSION     DAJTSF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.036"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..331)
                     /locus_tag="OO335_001824"
     CDS             complement(<1..331)
                     /locus_tag="OO335_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002234.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            701..1651
                     /gene="corA"
                     /locus_tag="OO335_001825"
     CDS             701..1651
                     /gene="corA"
                     /locus_tag="OO335_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR6002235.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1692..2072)
                     /locus_tag="OO335_001826"
     CDS             complement(1692..2072)
                     /locus_tag="OO335_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR6002236.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2086..2502)
                     /locus_tag="OO335_001827"
     CDS             complement(2086..2502)
                     /locus_tag="OO335_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002237.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="OO335_001828"
     CDS             complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="OO335_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6002238.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3503..3970)
                     /locus_tag="OO335_001829"
     CDS             complement(3503..3970)
                     /locus_tag="OO335_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6002239.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4135..5004
                     /gene="pldA"
                     /locus_tag="OO335_001830"
     CDS             4135..5004
                     /gene="pldA"
                     /locus_tag="OO335_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6002240.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5137..6966
                     /gene="recQ"
                     /locus_tag="OO335_001831"
     CDS             5137..6966
                     /gene="recQ"
                     /locus_tag="OO335_001831"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6002241.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7030..7650
                     /gene="rhtC"
                     /locus_tag="OO335_001832"
     CDS             7030..7650
                     /gene="rhtC"
                     /locus_tag="OO335_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR6002242.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7712..8332)
                     /gene="rhtB"
                     /locus_tag="OO335_001833"
     CDS             complement(7712..8332)
                     /gene="rhtB"
                     /locus_tag="OO335_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6002243.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8443..9465
                     /gene="pldB"
                     /locus_tag="OO335_001834"
     CDS             8443..9465
                     /gene="pldB"
                     /locus_tag="OO335_001834"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6002244.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9473..10273
                     /gene="yigL"
                     /locus_tag="OO335_001835"
     CDS             9473..10273
                     /gene="yigL"
                     /locus_tag="OO335_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6002245.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10349..11248
                     /gene="bioP"
                     /locus_tag="OO335_001836"
     CDS             10349..11248
                     /gene="bioP"
                     /locus_tag="OO335_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6002246.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11136..12089)
                     /gene="metR"
                     /locus_tag="OO335_001837"
     CDS             complement(11136..12089)
                     /gene="metR"
                     /locus_tag="OO335_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6002247.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12325..14586
                     /gene="metE"
                     /locus_tag="OO335_001838"
     CDS             12325..14586
                     /gene="metE"
                     /locus_tag="OO335_001838"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6002248.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14625..15440)
                     /locus_tag="OO335_001839"
     CDS             complement(14625..15440)
                     /locus_tag="OO335_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6002249.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15702..16463
                     /gene="udp"
                     /locus_tag="OO335_001840"
     CDS             15702..16463
                     /gene="udp"
                     /locus_tag="OO335_001840"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6002250.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16604..18031
                     /gene="rmuC"
                     /locus_tag="OO335_001841"
     CDS             16604..18031
                     /gene="rmuC"
                     /locus_tag="OO335_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6002251.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18126..18881
                     /gene="ubiE"
                     /locus_tag="OO335_001842"
     CDS             18126..18881
                     /gene="ubiE"
                     /locus_tag="OO335_001842"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6002252.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18895..19500
                     /gene="ubiJ"
                     /locus_tag="OO335_001843"
     CDS             18895..19500
                     /gene="ubiJ"
                     /locus_tag="OO335_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6002253.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19497..21137
                     /gene="ubiB"
                     /locus_tag="OO335_001844"
     CDS             19497..21137
                     /gene="ubiB"
                     /locus_tag="OO335_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6002254.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21216..21485
                     /gene="tatA"
                     /locus_tag="OO335_001845"
     CDS             21216..21485
                     /gene="tatA"
                     /locus_tag="OO335_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6002255.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21489..22004
                     /gene="tatB"
                     /locus_tag="OO335_001846"
     CDS             21489..22004
                     /gene="tatB"
                     /locus_tag="OO335_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6002256.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22007..22783
                     /gene="tatC"
                     /locus_tag="OO335_001847"
     CDS             22007..22783
                     /gene="tatC"
                     /locus_tag="OO335_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6002257.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22825..23607
                     /gene="tatD"
                     /locus_tag="OO335_001848"
     CDS             22825..23607
                     /gene="tatD"
                     /locus_tag="OO335_001848"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6002258.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23604..24092)
                     /gene="rfaH"
                     /locus_tag="OO335_001849"
     CDS             complement(23604..24092)
                     /gene="rfaH"
                     /locus_tag="OO335_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6002259.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24259..25752
                     /gene="ubiD"
                     /locus_tag="OO335_001850"
     CDS             24259..25752
                     /gene="ubiD"
                     /locus_tag="OO335_001850"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6002260.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25798..26499
                     /gene="fre"
                     /locus_tag="OO335_001851"
     CDS             25798..26499
                     /gene="fre"
                     /locus_tag="OO335_001851"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6002261.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="OO335_001852"
     CDS             complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="OO335_001852"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6002262.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="OO335_001853"
     CDS             complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="OO335_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6002263.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30243..31574
                     /gene="pepQ"
                     /locus_tag="OO335_001854"
     CDS             30243..31574
                     /gene="pepQ"
                     /locus_tag="OO335_001854"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6002264.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31574..32188
                     /locus_tag="OO335_001855"
     CDS             31574..32188
                     /locus_tag="OO335_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6002265.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32227..33678
                     /gene="trkH"
                     /locus_tag="OO335_001856"
     CDS             32227..33678
                     /gene="trkH"
                     /locus_tag="OO335_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6002266.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33690..34235
                     /gene="hemG"
                     /locus_tag="OO335_001857"
     CDS             33690..34235
                     /gene="hemG"
                     /locus_tag="OO335_001857"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6002267.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8108 a         8697 c         9302 g         8337 t
ORIGIN      
        1 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
       61 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
      121 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
      181 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggcaa
      241 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
      301 ttcctttacc aatgagtagc cgatgcgcca ttataggtcc tagatgtggg atttttttat
      361 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
      421 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
      481 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
      541 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt
      601 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
      661 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
      721 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
      781 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
      841 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
      901 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
      961 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
     1021 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg
     1081 taacgcctac gagttgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
     1141 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
     1201 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
     1261 agttcgcctg tgtctgatgg atacccagcg cgctctcaac ttcctggtgc gtaaagcgcg
     1321 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgcgatatcg aatccctgct
     1381 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
     1441 tatcaacatc gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct
     1501 gccgccaacg ctggttgctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
     1561 gtggagtttc ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
     1621 tctgtacttt aagcggaaga actggttgta aaaagaaggc ggtggcttag cctggctaag
     1681 ccacctgtta ttcaaaggct ccaggtattt aaccctttta cctctttctc ataaaaccat
     1741 ttgttcgtgt taacagcaac ataggctgct acggcaattc ccagaatgtt aacgccaatt
     1801 aatgcaccaa caaatagaga tagaatgcca agtaatagaa ctataattgc ttttttccat
     1861 aagcccagaa caaataaata gatccaggaa cagaagaaag caatgaaatt catttgaata
     1921 gttaatcgtt gtcttacttt taatgcttta aatgctgctt tatattctgg tgttgctccc
     1981 cagaatccag gaaagccatg ttgatcataa aaattaaatc ggtatttcca tttttcactt
     2041 aatgaatcat cgttcatata ttccttactc ataaatactc cataactatt gttttgatga
     2101 atcagtaggt gcaagcatta gcatactgaa agtggaaaaa taacaaatca aaaaaatcat
     2161 cgaaccattg cctgaacagg caaaatcttc ggctatcatt gtgatgatag agatgatata
     2221 tactgctaat gtaccaaaaa cataaatttt tatatatatg aaaccactat cacggagtcg
     2281 ttggcaattc atgttgatga cgagataatg gagtgcgatg gtagagacta taacaagaaa
     2341 acctgcttcc ccatcgttaa aaaagataat aagaaaggca aaaatgaaat ttattaaaat
     2401 aaataaaaat atatatcggt gtctggaaat cttaccgtta gatgttggga taaacatacg
     2461 taacataaat tttacatcct tgtgtgagtc tcctgtcagc atggcaatat gcccactctc
     2521 atacaagagt cggcatattt tttcagaata tatttatttt ttatttggac gttctacgct
     2581 gcgtataaat cgcgtccatc acaaaaattg ccagcgccac ccaaataaag gtgaaagtca
     2641 ccatcttatc ggcacccggt ttttcaccat aaaacgtcac agccagcagg aacatcagcg
     2701 tcgggccaat gtactgaaaa aagcctaacg ttgagagacg caggcgcgtg gcagcggcag
     2761 taaaacacaa cagcggtacg gtagtgacaa taccggcggc gatcagcagt aaattcagcg
     2821 acatcgggtt ttgccccata tggctggttg aactgtcggc aatagcaaac aggtaaatcg
     2881 ccgccacggg cagcagccac atggtttcaa ttaacatgcc ggtttgtgct tctaccgcaa
     2941 tcttcttgcg caccaggccg tagaaggcaa aactaaatgc cagccccagc gcgataatag
     3001 gtagcgaacc aaaagtccac agctggacca acacgccaca tatcgccaga atcaccgcca
     3061 gccattgcat ccggcggaat cgctcgccga ggaaaatcat ccccagcaca atgttcacca
     3121 gcgggttaat aaagtaacca aggctcgctt ccagcatatg gtgattgttc accgcccaga
     3181 taaacagtag ccagttgcca ccaatcagta cggcagagac tgccagcata aaaattttct
     3241 gtggcgtctg aatcagcgtt tttaaatagg accactggcg gcaaatgctc atcagcacca
     3301 ccataaagaa aaacgaccag atcacgcgat gcgtcaggat ctcatcggcg ggcacgtagt
     3361 aaatcagctt gaagtatgct ggcgcaatac cccaaataaa ataagcggca agagcgagta
     3421 atacgccctg ccgcgtttgt tttgcatcca tcgggaatac tcatttttaa ttggtaacag
     3481 cagtttacct gcttttatgt cttcaaccta ccatataggt ggcggtggca ctggcaatat
     3541 aaagctgttc ttcattgtgt aattcaacgc gggcgacggc gactttattg cctgcacgca
     3601 acaggctact ggtagcagta aaacgctcgc ccctgcctgg gcgcagataa tcaacgcgaa
     3661 gatcaatggt ccccatccgc gatagccgct ggcgtagttc atcttcactg atggtttcgt
     3721 ggcgggttaa ggtacttccc acgcacacca gaccggcggc gacatccagc gccgacgcaa
     3781 tgactccgcc gtgcaaaatg ctttgcgccc agttacccac catcatcggc tgatttttaa
     3841 aggccagctg tgcgaactct ttttcgtaac gctccagttc catccccaat gcgcggttaa
     3901 atggcatgtg ataaacaaac atctcaccca ctaatttcag ggcttgttca gcggtcagta
     3961 cggcagacat atcatcctta cacttcattg gttaatgaaa tgttgatttt atgcttcttt
     4021 gttgttggtt tctactttag gaagggataa ctaacggcaa tggagttaag tatgtaaaat
     4081 agcccgcaga aaaacattca ccttatcaat aattcgttac ggagaacacg accgatgcgg
     4141 actctgcagg gctggttgtt gccggtgttt atgttgccta tggcagtata tgcacaagag
     4201 gcaacggtga aagaggtgca tgacgcgcca gcggtgcgtg gcagtattat cgccaatatg
     4261 ctgcaggagc atgacaatcc gttcacgctc tatccttatg acaccaacta cctcatctac
     4321 acccaaacca gcgatctgaa taaagaagcg attgccagtt acgactgggc ggaaaatgcg
     4381 cgtaaggatg aagtaaagtt tcagttgagc ctggcatttc cgctgtggcg tgggatttta
     4441 ggcccaaact cggtgttggg tgcgtcttat acgcaaaaat cctggtggca actgtccaat
     4501 agcgaagagt cttcaccgtt tcgtgaaacc aactacgaac cgcaattgtt cctcggtttt
     4561 gccaccgatt accgttttgc aggttggacg ctgcgcgatg tggagatggg gtataaccac
     4621 gactctaacg ggcgttcaga cccgacctcc cgcagctgga accgccttta tactcgcctg
     4681 atggcagaaa acggtaactg gctggtagaa gtgaagccgt ggtatgtggt gggtaatact
     4741 gacgataacc cggatatcac caaatatatg ggttactacc agcttaaaat cggctatcac
     4801 ctcggtgatg cggtgctcag tgcgaaagga cagtacaact ggaacaccgg ctacggcggc
     4861 gcggagttag gcttaagtta cccgatcacc aaacatgtgc gcctttatac tcaggtttac
     4921 agcggctatg gcgaatcgct catcgactat aacttcaacc agacccgtgt cggtgtaggg
     4981 gttatgctaa acgacttgtt ttgatgaacg gttgagtggt tggcaaatct ggaatccagc
     5041 atccaggatt accctctcag agactaaaag cattgcagtt tctcgcgcag gcgctgaaaa
     5101 tagcgcctgt ttttatttca ggcaatcggg gtgaatgtgg cgcaggcgga agtgttgaat
     5161 ctggagtccg gagctaaaca ggttttacaa gaaacctttg gctaccaaca gtttcgcccc
     5221 ggccaggaag aaattatcga cactgtgctt tccggtcgcg attgcctggt tgttatgccc
     5281 actggtggcg gaaaatccct ttgctatcaa attcctgcct tattgctaaa cggccttacc
     5341 gtggttgttt caccgctgat ttcgttgatg aaagatcagg tggatcaact acaggccaac
     5401 ggcgtggcgg cggcgtgcct taactcgacg caaacccgcg aacagcaact tgaagtgatg
     5461 acaggctgcc gcaccgggca aattcgctta ctgtatatcg ccccggaacg cctgatgctg
     5521 gataactttc ttgagcatct ggcgcactgg aatccggtgt tattagccgt cgatgaagcg
     5581 cactgtatct cccaatgggg tcacgatttc cgcccggaat atgccgcgct cggtcagttg
     5641 cgccagcggt tcccgacgct gccgtttatg gcgctgaccg ccacagccga cgacaccacg
     5701 cgccaggata tcgtgcgcct gctggggctg aacgatccgc tgattcaaat cagcagtttt
     5761 gaccgtccga atattcgcta catgctgatg gagaaattca aaccgctcga tcagttgatg
     5821 cgctacgtgc aggaacagcg cggtaagtct ggcattatct actgcaacag ccgggcgaaa
     5881 gtagaagaca ccgctgcgcg cctgcaaagc aggggtatta gcgcggcggc ctatcatgcc
     5941 gggctggaaa ataatgtccg cgccgacgtg caggagaaat tccagcgcga tgatctgcaa
     6001 attgtggtgg cgacggtggc gttcggcatg ggcatcaata aaccaaacgt tcgcttcgtg
     6061 gtccactttg atattccgcg caatatcgaa tcctattatc aggaaaccgg tcgcgccggg
     6121 cgtgatggcc tgcctgcgga agcgatgctg ttttacgatc cggctgatat ggcgtggctg
     6181 cgccgttgtc tggaagagaa gccgcagggg cagttgcagg atatcgagcg ccacaaactc
     6241 aatgcgatgg gcgcgtttgc cgaagcgcaa acttgccgtc gtctggtatt gctgaactat
     6301 tttggcgaag ggcgtcagga gccgtgcggg aactgcgata tctgcctcga tccgccgaaa
     6361 cagtacgacg gttcaaccga tgctcagatt gccctttcca ccattggtcg tgtgaatcag
     6421 cggtttggga tgggttatgt ggtggaagtg attcgtggtg ctaataacca gcgtatccgc
     6481 gactatggtc atgacaaact gaaagtctat ggtatgggcc gtgataaaag ccatgaacat
     6541 tgggtgagcg tgatccgcca gctgattcat ctcggcctgg tgacgcaaaa tattgcccag
     6601 cattctgccc tacaactgac agaggccgcg cgcccggtgc tgcgcggcga atcctctttg
     6661 caacttgccg tgccgcgtat cgtggcgctc aaaccgaaag cgatgcagaa atcgttcggc
     6721 ggcaactatg atcgcaaact gttcgccaaa ttacgcaaac tgcgtaaatc gattgccgat
     6781 gaaagtaatg tcccgccgta cgtggtgttt aacgacgcaa ccttgattga gatggctgaa
     6841 cagatgccga tcaccgccag cgaaatgctc agcgttaacg gcgttgggat gcgcaagctg
     6901 gaacgctttg gtaaaccgtt tatggcgctg attcgtgcgc atgtcgatgg cgacgacgaa
     6961 gagtagtcag cagcataaaa aagtgccagt atgaagactc cgtaaacgtt tcccccgcga
     7021 gtcaaatgta tgttgatgtt atttctcacc gtcgccatgg tgcacattgt ggcgcttatg
     7081 agccccggtc ccgatttctt ttttgtctct cagaccgctg tcagtcgttc ccgtaaagaa
     7141 gcgatgatgg gcgtgctggg cattacctgc ggcgtaatgg tttgggctgg gattgcgttg
     7201 cttggcctgc atttgattat cgaaaaaatg gcctggctgc atacgctgat tatggtaggc
     7261 ggtggcctgt atctctgctg gatgggttac cagatgctac gcggtgcact gaaaaaagag
     7321 gctgtatctg cgcctgcacc acaggttgag ctggcgaaaa gcgggcgcag tttcctgaaa
     7381 ggcttactga ccaatctcgc caatccgaaa gcgattatct attttggttc ggtattctca
     7441 ttgtttgtcg gcgataacgt tggcactacc gagcgctggg gcattttcgc gttgattatt
     7501 gtcgaaacac tggcgtggtt taccgtcgtt gccagtctgt ttgccctgcc gcaaatgcgc
     7561 cgtggttatc aacgtctggc gaagtggatt gatggttttg ccggggcgtt atttgccgga
     7621 tttggcattc atttgattat ttcgcggtga tgccagacgc gtcttcagag taagtcggat
     7681 aaggcgttta cgccgcatcc gacattattt ttcacgcatg cctcgccgat gctaacagcg
     7741 ctcccaccag cataaacaac gagccgaaaa tcttattcag cgccttcatc tgctttggtc
     7801 ctttaatcca tagagcaatc ctttgagcaa gggtggcgta accgatcatc acaataatat
     7861 cgaccacaat agtggtgacg ccgagcacga tatactgcat cagttgcggc tgttgcggca
     7921 tgatgaattg cggaaatagc gccgccagaa acacaatact tttgggattg gtgagattca
     7981 caaaaactgc gcgctggaac aaatgtcgac gcgattgagt agaggccagc gatttaagat
     8041 caattgcgcc agcggcgcgc cactgctgga ttcccagcca aatcaagtaa gccgcgcctg
     8101 cccactttaa cacttcaaac gcaatcactg agcgggaaaa tagcgtcccc aaccccacgc
     8161 caaccagcac aatatgaatc gccagtccgg tctgaagccc agcaatagac gccaccgcgc
     8221 cgcgataacc gtggttgagc gaggtggtca tagtgttgat tgcaccagag cctggcgaca
     8281 ggcttaaaat gatcgatgtc agcaggtagg caaaccacca ttctaaggtc atgatgaact
     8341 cccggtgtgt ctatttttgt gccacaatac gctactgtcg cagcgttgtg tcaggcacgc
     8401 ttaaaaaaac gattttacgt ggtttaagag gcagattacc cgatgtttca gcagcaaaaa
     8461 gactgggaaa caagagaaaa cgcgtttgct gcttttacca tgggaccgct gactgatttc
     8521 tggcgtcagc gtgatgaagc agagtttact ggtgtggatg acattccggt gcgctttgtc
     8581 cgttttcgcg cacagcacca tgaccgggtg gtagtcatct gcccggggcg tattgagagc
     8641 tacgtaaaat atgcggaact ggcctacgac ctgttccatt tggggtttga tgtcttaatc
     8701 atcgaccatc gcgggcaggg acgttccggt cgcctgttag ccgatccgca tctcgggcat
     8761 gttaatcgct ttaatgatta tgttgatgat ctggcggcat tctggcagca ggaggttcag
     8821 cccggtccgt ggcgtaaacg ctatatactg gcacattcga tgggcggtgc gatctccaca
     8881 ttatttctgc aacgccatcc aggtgtatgt gacgccattg cgctaactgc gccaatgttt
     8941 gggatcgtga ttcgtatgcc gtcatttatg gcacggcaga tcctcaactg ggccgaagcg
     9001 catccacgtt tccgtgatgg ctatgcaata ggcaccgggc gctggcgcgc gttgccgttt
     9061 gctatcaacg tactgaccca cagcagacag cgatatcgac gtaacttacg cttctatgct
     9121 gatgacccaa cgattcgcgt cggtgggccg acctaccatt gggtacgcga aagtattctg
     9181 gctggcgaac aggtgttagc cggtgcgggt gatgacgcca cgcaaacgct tctcttacaa
     9241 gctgaagagg aacgcgtggt ggataaccgc atgcacgacc gtttttgtga actccgcacc
     9301 gccgcgggcc atcctgtcga aggaggacgg ccgttggtaa taaaaggtgc ttaccatgag
     9361 atcctttttg aaaaggacgc aatgcgctca gtcgcgctcc acgccatcgt tgattttttc
     9421 aacaggcata actcacccag cggaaaccgc tctacagagg tttaaatttc ttatgtacca
     9481 ggttgttgcg tctgatttag atggcacgtt actttctccc gaccatacgt tatcccctta
     9541 cgccaaagaa actctgaagc tgctcaccgc gcgcggcatc aactttgtgt ttgcgaccgg
     9601 tcgtcaccac gttgatgtgg ggcaaattcg cgataatctg gagattaagt cttacatgat
     9661 tacctccaat ggtgcgcgcg ttcacgatct ggatggcaat ctgatttttg ctcataacct
     9721 ggatcgcgac attgccagcg atctgtttgg cgtagtcaac gacaatccgg acatcattac
     9781 taacgtttat cgcgacgacg aatggtttat gaatcgccat cgcccggaag agatgcgctt
     9841 ttttaaagaa gcggtgttca aatatgcgct gtatgagccg ggattactgg agccggaagg
     9901 catcagcaaa gtgtttttca cctgcgattc ccatgaacaa ctgctgccgc tggagcaggc
     9961 gattaacgct cgctggggcg atcgcgtcaa cgtcagtttc tctaccttaa cctgtctgga
    10021 agtgatggcg ggcggcgtgt cgaaaggcca tgcgctggaa gcagtggcga agaaactggg
    10081 ctacagcctg aaggattgta ttgcgtttgg tgacgggatg aacgacgccg aaatgctgtc
    10141 gatggcgggg aaaggctgca ttatgggcag tgcgcaccag cgtctgaaag atctgcatcc
    10201 ggagctggaa gttattggta ctaatgccga cgacgcggtg ccgcattatc tgcgtaaact
    10261 ctatttatcg taatcgttct ttatttggtc agttgtcaac ctgatacttc gctacaatgg
    10321 atactcgtta atcaaagagt tttccattgt ggcgttactt atcatcacca cgattctgtg
    10381 ggccttctcc tttagctttt atggcgagta ccttgcgggg cacgtcgata gctattttgc
    10441 ggtgctggtg cgcgttggct tggcggcact cgtttttctg ccgtttctgc gtacccgtag
    10501 caatagcctg aaaacggtcg gcctgtatat gctggtgggc gcgatgcagc tcggcgtgat
    10561 gtatatgctg agtttccgcg cttatctcta cctgacggtc tccgaattgt tgctgttcac
    10621 cgtactgacg ccgctctaca tcacgctgat ttatgacatc atgagtaagc gccgtctgcg
    10681 ctggggctat gcttttagcg ccttgctggc ggtgattggt gccgggatta ttcgctatga
    10741 tcaggtcacc gaccatttct ggactggctt gttgctggtg caactctcca atatcacttt
    10801 tgccatcggc atggtgggtt acaaacgcct gatggaaact cgcccgatgc cgcagtataa
    10861 cgcctttgcg tggttctatc ttggcgcgtt cctggtggcg gtaattgcat ggttcttgct
    10921 gggaaatgcg cagaaaatgc cgcaaaccac gttgcaatgg ggcattctgg tgtttcttgg
    10981 cgtggtggct tccgggattg gctactttat gtggaactac ggcgcgacgc aggtggacgc
    11041 cggaacgctg ggcattatga ataatatgca cgttccggca gggctgctgg taaacctggc
    11101 tatctggcac caacagccgc actggccaac gtttattaca ggcgcgctgg tgatcctggc
    11161 ctcactgtgg gtgcatcgta agtgggtcgc tccgcgctct tcacaaacgg cagatgatcg
    11221 caggcgtgat tgcgcgctga gcgaataaac gcttccgtaa ctggctgacg ctgctcgcca
    11281 tcgcgcacgg cggcgtacag tcggctccac aagccttcgc ccagggtttt ggtcaccacc
    11341 agaccctggc gctcaaaact ctctactacc caatgcggta gcgcggcaat acccatccgc
    11401 gcggcaacca tttgaatcaa caataaggtg ttatcgacgc ttttcagtga cgggctgacg
    11461 cctgccggct gaagaaaatg ccgccagaca tccagtcgac tacgctgcac cggataaatt
    11521 aatagcgtct cgctggcgag atcttccggt gtaatacgcg tttttgccgc cagtggatgg
    11581 tcaggtgcta acaccaggcg tacttcataa tcgaacatcg gcgaataatg caggccactg
    11641 cgcggcagga tatcggaggt catcaccaga tccagctcgc cctgttgcaa cgcgggctgc
    11701 gggtcaaacg tcacgcccga tttaaaatcc atctctacct gcggccagtt cttatggaaa
    11761 tttcctaaag cgggtgtcag ccactgaata cagctatggc actcaatggc aatgcgcaga
    11821 cgcgtctgct gcggttcatt gcaggcttgc agtgcctggc tgatttgcgg cagcacctgg
    11881 tttgccagtt gcaacaggat ttctccctgc ggtgtaaagc gtagcggctg gctcttacgc
    11941 acaaatagcc ggaagccaag gcgttgttcc agatcgctaa actggtgaga cagggcggat
    12001 tgcgtctgat gcaacgttgc cgcagcggct gcgagcgagc cgcagttccg caacgcttgt
    12061 agcgttttca ggtgttttac ttcgatcatg aaagtccttc acttcggcat gaataatttg
    12121 cgcttgagga atatacagta accgccaatt atggatgtgt aaacatctgg acggctaaaa
    12181 tcattcgtct tttaaattta tggtgcgttg gctgcgtttc tccaccccgg tcacttactt
    12241 cagtaagctc ccggcgatga ataaacttgc cgccttccct aaattcaaaa tccataggat
    12301 ttacatataa ttagaggaag aaaaatgaca atattgaatc acaccctcgg tttccctcgc
    12361 gttggcctgc gtcgcgagct gaaaaaagcg caagaaagtt attgggcggg gaactccacg
    12421 cgtgaagaac tgctgacagt agggcgtgaa ctgcgtgctc gtcactggga tcaacaaaag
    12481 caagcgggta tcgacctgct gccggtgggc gattttgcct ggtacgatca tgtactgacc
    12541 accagtttgc tgctgggtaa tgttccgcca cgtcatcaga acaaagacgg ttcggtagat
    12601 atcgacaccc tgttccgtat tggtcgtgga cgtgcgccga ctggcgaacc tgcggcggca
    12661 gcggaaatga ccaaatggtt taacaccaac tatcactaca tggtgccgga gttcgttaaa
    12721 ggccaacagt tcaaactgac ttggacgcag ctgctggagg aagtggacga ggcgctggcg
    12781 ctgagccaca acgttaagcc tgtgctgctg gggccggtta cttacctgtg gctggggaaa
    12841 gtgaaaggtg aacagtttga ccgccttagc ctgctgaacg acattctgcc ggtttatcag
    12901 caagtgctgg cagaactggc gaaacgcggc atcgagtggg tacagattga tgaacccgcg
    12961 ctggtactgg aactaccaca ggcgtggctg gacgcataca aacccgctta cgacgcgctc
    13021 cagggacagg tgaaactgct gctgaccacc tattttgaag gcgtaacgcc aaatctcgac
    13081 acgattactg cgctgcctgt tcaggggctg catgtggacc ttgtacatgg taaagataac
    13141 gttgttgaac tgcacaagcg cctgccttct gactggctgc tgtctgcggg tctgatcaat
    13201 ggtcgtaacg tctggcgcgc cgatcttact gagaaatatg cgcaaattaa ggacattgtc
    13261 ggcaaacgtg atttgtgggt ggcatcttcc tgctcgttgc tgcacagccc catcgacctg
    13321 agcgtggaaa cgcgtcttga tgcagaagtg aaaagctggt ttgccttcgc cctacaaaaa
    13381 tgccatgaac tggcactgct gcgcgatgcg ctgaacagtg gtgacacggc agctctggca
    13441 gagtggagcg ccccaattca ggcgcgtcgt cactctactc gtgtacataa tccggcggta
    13501 gaaaagcgtc tggcggcgat caccgcccag gacagccagc gtgcgaatgt ctatgaagtg
    13561 cgtgctgaag cccagcgtgc gcgttttaaa ctgccagcgt ggccgaccac cacgattggt
    13621 tccttcccgc aaaccacgga aattcgtacc ctgcgtctgg atttcaaaaa gggcaatctc
    13681 gacgccaaca actaccgcac gggcattgcg gaacatatca agcaggccat tgttgagcag
    13741 gaacgtttgg gactggatgt gctggtacat ggcgaggccg agcgtaatga catggtggaa
    13801 tactttggcg agcacctcga cggatttgtc tttacgcaaa acggttgggt acagagctac
    13861 ggttcccgct gcgtgaagcc accgattgtc attggtgaca ttagccgccc ggcaccgatt
    13921 accgtggagt gggcgaagta tgcgcaatcg ctgaccgaca aaccggtgaa agggatgctg
    13981 acggggccgg tgaccatact ctgctggtcg ttcccgcgtg aagatgtcag ccgtgaaacc
    14041 atcgccaaac agattgcgct ggcgctgcgt gatgaagtgg ccgatctgga agccgctgga
    14101 attggcatca tccagattga cgaaccggcg ctgcgcgaag gtttaccgct gcgtcgtagc
    14161 gactgggatg cgtatctcca gtggggcgta gaggccttcc gtatcaacgc cgccgtggcg
    14221 aaagatgaca cacaaatcca cactcacatg tgttattgcg agttcaacga catcatggat
    14281 tcgattgcgg cgctggacgc agacgtcatc accatcgaaa cctcgcgttc cgacatggag
    14341 ttgctggagt cgtttgaaga gtttgattat ccaaatgaaa tcggtcctgg cgtctatgac
    14401 attcactcgc caaacgtacc gagcgtggaa tggattgaag ccttgctgaa gaaagcggca
    14461 aaacgcattc cggcagagcg cctgtgggtc aacccggact gtggcctgaa aacgcgcggc
    14521 tggccagaaa cccgcgcggc actggcgaac atggtgcagg cggcgcagaa tttgcgtcgg
    14581 gggtaaatcc aaaccgggtg gtaataccac ccggtctttt ctcattacag cgacttcttc
    14641 ccaccatact gcttaaacca ttccagcata cgctgccagc catctttcgc agattcggca
    14701 tgatagctcg ggcgataatc agcgttgaat gcatgcccgg catccgggta cacgataatc
    14761 tctgctttcg cattagcagc ccgcagcgcc tggcgcatcg tttcaacgct ctcctgcgga
    14821 atgctgttat cctgaccacc atataagccg agaaccggcg cgttaagatc ggttgcgata
    14881 tcaacaggtt gtttcggtga attcagcgac ttgtcgcccg tcagtttgcc gtaccacgcc
    14941 actgcggctt ttagctgtgg attatgcgcg gcatacagcc aggtgatacg tccaccccag
    15001 cagaatccgg tgatcattaa acgatgaaca tcgccgccgt tgcgggaagc ccaactggcg
    15061 acatgatcga gatcggccag cacctgcgag tcaggcactt ttgctaccag accgctaagc
    15121 aacgtgggga tatcggcaaa atcattcgga tcgccttcgc ggaagtaaag ttcaggtgcg
    15181 atagccagat acccctccag cgccagacgg cgacaaatgt cgcggatatg ttcatgcacg
    15241 ccaaaaattt cctgcactac aatgaccact ggcagtgggc catcgctttg ctttggtctg
    15301 gcatggtaag caggcatgtt atccccttgt gaagggatag aggtgaagcc cgccacaatc
    15361 gcgtcgtccg gggtctgaac gatggtcgaa gcgagaggcg atgcagcagg tgcaaatcca
    15421 gattgttgtg ttgttgccat ggtattctcc gtacccttat aaaaatgttg cgcaatgtta
    15481 actatagtca gcatgcaaca aatcacattg cctgaatcgg ctcatctttt atgcagtcct
    15541 gcagaatgaa gagtgattta tgtgatatga atcacttttg gtgggtaaat ttatgcaacg
    15601 catttgcgtc atggtgatga gtatcacgaa aaaatgttaa acccttcggt aaagtgtctt
    15661 tttgcttctt ctgactaaac cgattcacag aggagttgta tatgtccaag tctgatgttt
    15721 ttcatctcgg cctcactaaa aacgatttac aaggggctac gcttgccatc gtccctggcg
    15781 acccggatcg tgtggaaaag atcgccgcgc tgatggataa gccggttaag ctggcatctc
    15841 accgcgaatt cactacctgg cgtgcagagc tggatggtaa acctgttatc gtctgctcta
    15901 ccggtatcgg cggcccgtct acctctattg ctgttgaaga gctggcacag ctgggcattc
    15961 gcaccttcct gcgtatcggt acaacgggcg ctattcagcc gcatattaat gtgggtgatg
    16021 tcctggttac cacggcgtct gtccgtctgg atggcgcgag cctgcacttc gcaccgctgg
    16081 aattcccggc tgtcgctgat ttcgaatgta cgactgcgct ggttgaagct gcgaaatcta
    16141 ttggcgcgac aactcacgtt ggcgtgacag tttcttctga taccttctac ccaggtcagg
    16201 aacgttacga tacttactct ggtcgcgtag ttcgtcactt taaaggttct atggaagagt
    16261 ggcaggcgat gggcgtaatg aactatgaaa tggaatctgc aactctgctg accatgtgtg
    16321 caagtcaggg cctgcgtgcc ggtatggtag cgggtgttat cgttaaccgc acccagcaag
    16381 agatcccgaa tgctgagacg atgaaacaaa ccgaaagcca tgcggtgaaa atcgtggtgg
    16441 aagcggcgcg tcgtctgctg taattctctt ctcctgtctg aaggccgacg cgttcggcct
    16501 tttgtatttt tgcgtagcgc ctcgcaggaa atgcctttcc aactggacgt ttgtacagca
    16561 caattctatt ttgtgcgggt aagttgttgc gtcaggaggc gttgtggatt tctcaatcat
    16621 ggtttacgca gttattgcgt tggtgggtgt ggcaattggc tggctgtttg ccagttatca
    16681 acatgcgcag caaaaagccg agcaattagc tgaacgtgaa gagatggtcg cggagttaag
    16741 cgcggcaaaa caacaaatta cccaaagcga gcactggcgt gcagagtgtg aattgctcaa
    16801 taacgaagtg cgcagcctgc aaagtattaa cacctctctg gaggccgatc tgcgtgaagt
    16861 aaccacgcgg atggaagccg cacagcaaca tgctgacgat aaaattcgcc agatgattaa
    16921 cagcgagcag cgcctcagtg agcagtttga aaacctcgcc aaccgtattt ttgagcacag
    16981 caatcgccgg gttgatgagc aaaaccgtca gagtctgaac agcctgttgt cgccgctacg
    17041 tgaacaactg gacggtttcc gccgtcaggt tcaggacagc ttcggtaaag aagcacaaga
    17101 acgccatacc ctgacccacg aaattcgcaa tctccagcaa ctcaacgcgc aaatggccca
    17161 ggaagcgatc aacctgacgc gcgcgctgaa aggcgacaat aaaacccagg gcaactgggg
    17221 cgaggtagta ttgacgcggg tgctggaggc ttccggtctg cgtgaagggt atgaatatga
    17281 aacccaggtc agcatcgaaa atgacgcccg ctcgcggatg cagccggatg tcatcgtgcg
    17341 gctgccgcag ggaaaagatg tggtgatcga cgccaaaatg acgctggtcg cctatgaacg
    17401 ctattttaac gccgaagacg actacacccg cgaaagcgcg ctacaggaac atatcgcgtc
    17461 ggtgcgtaac catatccgtt tgctgggacg caaagattat caacagctgc cggggctgcg
    17521 aactctggat tacgtgctga tgtttattcc cgttgaaccc gcttttttac tggcgcttga
    17581 ccgccagccg gagctgatca ccgaagcgtt gaaaaacaat atcatgctgg ttagcccgac
    17641 tacgctgctg gtggcgttgc gtactattgc caacctgtgg cgttatgagc atcaaagccg
    17701 taacgcccag caaatcgccg atcgtgccag caagctgtac gacaagatgc gtttgttcat
    17761 cgatgacatg tccgcgattg gtcaaagtct cgacaaagcg caggataact accgccaggc
    17821 aatgaaaaaa ctctcttcag gtcgcggaaa tgtgctggcg caggcagaag cgtttcgcgg
    17881 tttaggagta gaaattaaac gcgagattaa tccggatttg gctgaacagg cggtgagcca
    17941 ggatgaagag tatcgacttc ggtcggttcc ggagcagccg aatgatgaag cttatcaacg
    18001 cgatgatgaa tataatcagc agtcgcgcta gcccattagg ggtagttaag ccgggtagaa
    18061 atctagggca tcgacgccca atctgttaca cttctggaac aattttttga tgagcaggca
    18121 ttgagatggt ggataagtca caagaaacga cgcactttgg ttttcagacc gtcgcgaagg
    18181 aacaaaaagc ggatatggtc gcccacgttt tccattccgt ggcatcaaaa tacgatgtca
    18241 tgaatgattt gatgtcattt ggtattcatc gtttgtggaa gcgattcacg attgattgca
    18301 gcggcgtacg ccgtgggcag accgtgctgg atctggctgg tggcaccggc gacctgacag
    18361 cgaaattctc ccgcctggtc ggagaaactg gcaaagtggt ccttgctgat atcaatgaat
    18421 ccatgctcaa aatgggacgc gagaagctgc gtaatatcgg tgtgattggc aacgttgagt
    18481 atgttcaggc gaacgctgag gcgctgccgt tcccggataa cacctttgat tgcatcacca
    18541 tttcgtttgg tctgcgtaac gtcaccgaca aagataaagc actgcgttca atgtatcgcg
    18601 tgctgaaacc cggcggccgc ctgctggtgc ttgagttctc gaagccaatt atcgagccgc
    18661 tgagcaaagc ctatgatgca tactccttcc atgtgctgcc gcgtattggc tcactggtcg
    18721 cgaacgacgc cgacagctac cgttatctgg cagaatccat ccgtatgcat cccgatcagg
    18781 ataccctgaa agccatgatg caggatgccg gattcgaaag tgtcgactac tacaatctga
    18841 cggcaggggt tgtggcgctg catcgtggtt ataagttctg acaggagacc ggaaatgcct
    18901 tttaaacctt tagtgacggc aggaattgaa agtctgctca acaccttcct gtatcgctca
    18961 cccgcgctga aaacagcccg ctcgcgtctg ctgggtaaag tattgcgcgt ggaggtaaaa
    19021 ggcttttcga cgtcattgat tctggtgttc agcgaacgcc aggttgatgt actgggcgaa
    19081 tgggcaggcg atgctgactg caccgttatc gcctacgcca gtgtgttgcc gaaacttcgc
    19141 gatcgccagc agcttaccgc actgattcgc agtggtgagc tggaagtgca gggcgatatt
    19201 caggtggtgc aaaacttcgt tgcgctggca gatctggcag agttcgaccc tgcggaactg
    19261 ctggcccctt ataccggtga tatcgccgct gaaggaatca gcaaagccat gcgcggaggc
    19321 gcaaagttcc tgcatcacgg cattaagcgc cagcaacgtt atgtggcgga agccattact
    19381 gaagagtggc gtatggcacc cggtccgctt gaagtggcct ggtttgcgga agagacggct
    19441 gccgtcgagc gtgctgttga tgccctgacc aaacggctgg aaaaactgga ggctaaatga
    19501 cgccaggtga agtacggcgc ctatatttca tcattcgcac ttttttaagc tacggacttg
    19561 atgaactgat ccccaaaatg cgtatcaccc tgccgctacg gctatggcga tactcattat
    19621 tctggatgcc aaatcggcat aaagacaaac ttttaggtga gcgactacga ctggccctgc
    19681 aagaactggg gccggtttgg atcaagttcg ggcaaatgtt atcaacccgc cgcgatcttt
    19741 ttccaccgca tattgccgat cagctggcgt tattgcagga caaagttgct ccgtttgatg
    19801 gcaagctggc gaagcagcag attgaagctg caatgggcgg cttgccggta gaagcgtggt
    19861 ttgacgattt tgaaatcaag ccgctggctt ctgcttctat cgcccaggtt cataccgcgc
    19921 gattgaaatc gaatggtaaa gaggtggtga ttaaagtcat ccgcccggat attttgccgg
    19981 tcattaaagc ggatctgaaa cttatctacc gtctggctcg ctgggtgccg cgtttgctgc
    20041 cggatggtcg ccgtctgcgc ccaaccgaag tggtgcgcga gtacgaaaag accttgattg
    20101 atgaactgaa tttgctgcgg gaatctgcca acgccattca gcttcggcgc aattttgaag
    20161 acagcccgat gctctacatc ccggaagttt accctgacta ttgtagtgaa gggatgatgg
    20221 tgatggagcg catttacggc attccggtgt ctgatgttgc ggcgctggag aaaaacggca
    20281 ctaacatgaa attgctggcg gaacgcggcg tgcaggtgtt cttcactcag gtctttcgcg
    20341 acagcttttt ccatgccgat atgcaccctg gcaacatctt cgtaagctat gaacacccgg
    20401 aaaacccgaa atatatcggc attgattgcg ggattgttgg ctcgctaaac aaagaagata
    20461 aacgctatct ggcagaaaac tttatcgcct tctttaatcg cgactatcgc aaagtggcag
    20521 agctacacgt cgattctggc tgggtgccac cagataccaa cgttgaagag ttcgaatttg
    20581 ccattcgtac ggtctgtgaa cctatctttg agaaaccgct ggccgaaatt tcgtttggac
    20641 atgtactgtt aaatctgttt aatacggcgc gtcgcttcaa tatggaagtg cagccgcaac
    20701 tggtgttact ccagaaaacc ctgctctatg tcgaaggggt aggacgccag ctttatccgc
    20761 agctcgattt atggaaaaca gcgaagcctt tcctggagtc gtggattaaa gatcaggtcg
    20821 gtattcctgc tctggtgaga gcatttaaag aaaaagcgcc gttctgggtc gaaaaaatgc
    20881 cagaactgcc tgaactggtt tacgacagtt tgcgccaggg caagtattta cagcacagtg
    20941 ttgataagat tgcccgcgag cttcagtcaa atcatgtacg tcagggacaa tcgcgttatt
    21001 ttctcggaat tggcgctacg ttagtattaa gtggcacatt cttgttggtc agccgacctg
    21061 agtgggggct gatgcccggc tggttaatgg caggtggtct gatcgcctgg tttgtcggtt
    21121 ggcgcaaaac acgctgattt tttcatcgct caaggcgggc cgtgtaacgt ataatgcggc
    21181 tttgtttaat catcatctac cacagaggaa catgtatggg tggtatcagt atttggcagt
    21241 tattgattat tgccgtcatc gttgtactgc tttttggcac caaaaagctc ggctccatcg
    21301 gttccgatct tggtgcgtcg atcaaaggct ttaaaaaagc aatgagcgat gatgaaccaa
    21361 agcaggataa aaccagtcag gatgctgatt ttactgcgaa aactatcgcc gataagcagg
    21421 cggatacgaa tcaggaacag gctaaaacag aagacgcgaa gcgccacgat aaagagcagg
    21481 tgtaatccgt gtttgatatc ggttttagcg aactgctatt ggtgttcatc atcggcctcg
    21541 tcgttctggg gccgcaacga ctgcctgtgg cggtaaaaac ggtagcgggc tggattcgcg
    21601 cgttgcgttc actggcgaca acggtgcaga acgaactgac ccaggagtta aaactccagg
    21661 agtttcagga cagtctgaaa aaggttgaaa aggcgagcct cactaacctg acgcccgaac
    21721 tgaaagcgtc gatggatgaa ctacgccagg ccgcggagtc gatgaagcgt tcctacgttg
    21781 caaacgatcc tgaaaaggcg agcgatgaag cgcacaccat ccataacccg gtggtgaaag
    21841 ataatgaagc tgcgcatgag ggcgtaacgc ctgccgctgc acaaacgcag gccagttcgc
    21901 cggaacagaa gccagaaacc acgccagagc cggtggtaaa acctgctgcg gacgctgaac
    21961 cgaaaaccgc tgcaccttcc ccttcgtcga gtgataaacc gtaaacatgt ctgtagaaga
    22021 tactcaaccg cttatcacgc atctgattga gctgcgtaag cgtctgctga actgcattat
    22081 cgcggtgatc gtgatattcc tgtgtctggt ctatttcgcc aatgacatct atcacttggt
    22141 atccgcgcca ttgatcaagc agttgccgca aggttcaacg atgatcgcca ccgacgtggc
    22201 ctcgccgttc tttacgccga tcaagctgac ctttatggtg tcgctgattc tgtcagcgcc
    22261 ggtgattctc tatcaggtgt gggcatttat cgccccagcg ctgtataagc atgaacgtcg
    22321 cctggtggtg ccgctgctgg tttccagctc tctgctgttt tatatcggca tggcattcgc
    22381 ctactttgtg gtctttccgc tggcatttgg tttccttgcc aataccgcgc cggaaggggt
    22441 gcaggtatcc accgacatcg ccagctattt aagcttcgtt atggcgctgt ttatggcgtt
    22501 tggtgtctcc tttgaagtgc cggtggcaat tgtgctgctg tgctggatgg ggattacctc
    22561 gtcagaagac ttacgcaaaa aacgcccgta tgtgctggtt ggtgcattcg ttgtcgggat
    22621 gttgctgacg ccgccggatg tcttctcgca aacgctattg gcgatcccga tgtactgtct
    22681 gtttgaaatc ggtgtcttct tctcacgctt ttacgttggt aaagggcgaa atcgggaaga
    22741 ggaaaacgac gctgaagcag aaagcgaaaa aactgaagaa taaattcaac cgcccgtcag
    22801 ggcggttgtc atatggagta caggatgttt gatatcggcg ttaatttgac cagttcgcaa
    22861 tttgcgaaag accgtgatga tgttgtagcg cgcgcttttg acgcgggagt taatgggcta
    22921 ctcatcaccg gcactaacct gcgtgaaagc cagcaggcgc aaaagctggc gcgtcagtat
    22981 tcgtcctgtt ggtcaacggc gggcgtacat cctcacgaca gcagccagtg gcaagctgcg
    23041 actgaagaag cgattattga gctggccgcg cagccagaag tggtggcgat tggtgaatgt
    23101 ggtctcgact ttaaccgtaa cttttcgacg ccggaagagc aggaacgcgc ttttgttgct
    23161 cagctacgca ttgccgcaga attaaacatg ccggtattta tgcactgtcg cgatgcccac
    23221 gagcggttta tgacattgct ggagccgtgg ctggataaac tgcctggtgc ggttcttcat
    23281 tgctttaccg gcacacgcga agagatgcag gcgtgcgtgg cgcgtggaat ttatatcggc
    23341 attaccggtt gggtttgcga tgaacgacgc gggctggagc tgcgggaatt gttgccgttg
    23401 attccggcgg agaaattgct gatcgaaaca gatgcgccgt atctgctccc gcgcgatctc
    23461 acgccaaagc catcatcccg gcgcaacgag ccagcccatc tgccccatat tttgcaacgt
    23521 attgcgcact ggcgtggaga agatgccgca tggctggctg ccaccacgga tgccaatgtc
    23581 aaaacactgt ttgggattgc gttttagagt ttgcggaact cggtgttctt cacactgtgc
    23641 ttaatctctt tattaataag attaagcaat agcatggagc gagcctcacc atcgggttcg
    23701 gtgaaaatgg cctgaaagcc ttcgaacgcg ccttcggtaa taatcacctt atcacccgga
    23761 taaggggggg ccggatcgac aatgtctttc ggtttatata ccgatagctg atgaattacc
    23821 gccgatggga ctatcgctgg cgacgcgcca aagcgcacaa agtggctgac gccacgcgta
    23881 gcgctgatgg tcgtggtatg aatcacttct ggatcaaatt ccacaaacag gtagttaggg
    23941 aataacggtt cactgactgc ggtacgttta ccccgtacga ttttttccag ggtgatcatc
    24001 ggagcgaggc aattaaccgc ctgtctctca aggtgttcct gggcacgttg aagttgcccg
    24061 cgcttgcagt acagtaaata ccaggattgc ataatgactc ttatccgttt aatcggggcg
    24121 caaggatagc aaaagcttta cgctaagtta attatattcc ccggtttgcg ttataccgtc
    24181 agagttcacg ctaatttaac aaatttacag cattgcaaag atgaacgccg tataatgggc
    24241 gcagattaag aggctacaat ggacgccatg aaatataacg atttacgcga cttcctgacg
    24301 ctgcttgaac agcagggtga gctaaaacgt atcacgctcc cggtggatcc gcatctggaa
    24361 atcactgaaa ttgctgaccg cactctgcgt gctggtgggc ctgcgctgtt gttcgaaaac
    24421 cctaaaggct actcaatgcc ggtgctgtgc aacctgttcg gtacgccaaa gcgcgtggcg
    24481 atgggcatgg ggcaggaaga tgtttcggcg ctgcgtgaag ttggtaaatt attggcgttt
    24541 ctgaaagagc cggagccgcc aaaaggtttc cgcgacctgt ttgataaact gccgcagttt
    24601 aagcaggtgt taaacatgcc gacaaagcga ctgcgtggtg cgccctgcca acaaaaaatc
    24661 gtctctggcg atgacgtcga tctcaatcgc attcccatta tgacctgctg gccggaagat
    24721 gccgcgccgc tgattacctg ggggctgacc gtaacgcgcg gcccgcataa agagcggcag
    24781 aatctgggca tttatcgcca gcagctgatt ggtaaaaaca aactgattat gcgctggctg
    24841 tcgcatcgcg gcggcgcgct ggattatcag gagtggtgtg cggcgcatcc gggcgaacgt
    24901 ttcccggttt ctgtggcgct gggtgccgat cctgccacga ttctcggtgc agtcaccccc
    24961 gttccggata cgctttcaga gtatgcgttt gccggattgc tacgcggcac caaaaccgaa
    25021 gtagtaaagt gtatttccaa tgacctcgaa gtgccagcca gtgccgaaat cgtcctggaa
    25081 gggtatatcg atcctggtga gatggcgccg gaagggccgt atggtgacca cacaggttac
    25141 tataatgaag tcgataattt cccggtgttt accgtgacgc atattaccca gcgtgaagat
    25201 gcgatttacc attccaccta taccgggcgt ccgccagatg agcccgcggt actgggcgtg
    25261 gcgttgaacg aagtgtttgt accgattctg caaaaacagt tcccggaaat tgtcgatttt
    25321 tacctgccgc cggaaggctg ttcttatcgt ctggcggtag tgacgatcaa aaaacagtac
    25381 gccggacacg cgaagcgcgt catgatgggc gtctggtcgt tcttacgcca gtttatgtac
    25441 actaaatttg tgatcgtttg cgatgatgac gtcaacgcac gcgactggaa cgatgtgatt
    25501 tgggcgatta ccacccgtat ggacccggcg cgggatactg ttctggtaga aaatacgcct
    25561 attgattatc tggattttgc ctcgcctgtc tctgggctgg gttcaaaaat ggggctggat
    25621 gccacgaata aatggccggg ggaaacccag cgtgaatggg gacgtcccat caaaaaagat
    25681 ccagatgttg tcgcgcatat tgacgccatc tgggatgaac tggctatttt taacaacggt
    25741 aaaagcgcct gatgcgcgtt tgttttgccc tatttatcga tccgacagag aaagcgcatg
    25801 acaaccttaa gctgtaaagt gacctcggta gcagctatca cggataccgt atatcgtgtc
    25861 cgcatcgtgc cagacgcggc cttttctttt cgtgctggtc agtatttgat ggtagtgatg
    25921 gatgagcgcg acaaacgtcc gttctcaatg gcctcgacgc cggatgaaaa agggtttatc
    25981 gagctgcata ttggcgcttc tgaaatcaac ctttacgcga aagcagtcat ggaccgcatc
    26041 ctcaaagatc atcaaatcgt ggtcgacctt ccccacggag aagcgtggct gcgcgatgat
    26101 gaagagcgtc cgatgatttt gattgcgggc ggcaccgggt tctcttatgc ccgctcgatt
    26161 ttgctgacag cgttggcgcg taacccaaac cgtgatatca ccatttactg gggcgggcgt
    26221 gaagagcagc atctgtatga tctctgcgag cttgaggcgc tttcgttaaa gcaccttggt
    26281 ctgcaagtgg tgccggtggt tgaacaaccg gaagcaggct ggcgtgggcg tactggtacc
    26341 gtgttaacgg cggtattgca ggatcatggt acgctggcag agcatgatat ctatattgcc
    26401 ggacgttttg agatggcgaa aattgcccgt gacctgtttt gcagtgagcg taatgcgcgg
    26461 gaagatcgcc tgtttggcga tgcgtttgca tttatctgag atataaaaaa acccgcccct
    26521 gacaggcggg aagaacggca actaaaactt tattcagtgg catttagatc tatgtcgcat
    26581 ccggcaattg gtgcacgatg cctgatgcga cgctggcgcg tcttatcagg cctacattgg
    26641 tgccggatcg gtagaccgga taaggcgttc acgccgcatc cggcaagtgg ttaaacccgc
    26701 tcaaacaccg tcgcaatacc ctgacccaga ccgatacaca tcgtcgccag accaaactga
    26761 acgtctttgc gttccatcag attcagcagc gtggtgctga tacgcgcacc ggaacaaccc
    26821 agcggatgac ccagcgcgat cgcaccacca ttgaggttga tcttctcgtc aatctgctcc
    26881 attagcccga gatctttaat gcacggcagg atctgcgcgg caaaggcttc gttcatctca
    26941 aacacgccga tatcgctggc agaaagcccc gcttttttca gcgccagctt cgaggccgga
    27001 accgggccgt aacccataat cgatgggtca caaccaacga ccgccatcga acgcacacga
    27061 gcgcgcggct taagacctaa ttcatgggcg cggctttcac tcatcaccag catggcagct
    27121 gcgccatcgg aaagtgcaga agatgtgccc gccgttaccg taccgtttac tggatcaaac
    27181 gccggacgca gcgtggcgag ggcttccacg gtggtttccg ggcgaatcac ttcgtcgtaa
    27241 ttaaactgct tcaggacgcc gtcggcatcg tgaccaccag ttgggatgat ttcattttta
    27301 aatgcggccg actgcgtggc ggcccaggcg cgggcgtgtg accgcgcggc aaaggcatcc
    27361 tgcatttcac ggctgatacc gtgcatacgc gccagcattt ctgccgttaa gcccatcatg
    27421 cccgccgctt tggcgacatt gcggctcagg ccggggtgaa aatcgacgcc gtgactcatc
    27481 ggcacatggc ctatatgctc cacgccgcca accagacatg cctgcgtatc gccagtcatg
    27541 atcattcgtg ctgcgtcatg cagtgcctgc atggatgaac cacacaagcg attgacggta
    27601 accgccggga cagagtgcgg cacttctgcc agcagcgccg cgttacgggc gatgttaaaa
    27661 ccctgctcca gcgtctgttg cacacaaccc cagtaaatat cgtcgagggc cgccgcttcc
    27721 agcgccgggt tacgcgccag caggctacgc attaaatgag cggagagatc ttccgcacgc
    27781 acgttacgaa aagcaccgcc cttcgaacgg cccatcgggg tgcgaattgc atcgacaatg
    27841 acaacctgtt ccattgtgac tccttaagcc gttttcaggt cgccaaccgg acgggctggc
    27901 tcaaccggag gatagtacgg ttcgttatga cgcgctttat tacgcagacc ttccggcact
    27961 tcatacagcg ggccgaggtg ctgatattgc tgtgccatat cgaggtattt tgcgctaccg
    28021 agggtgtcca gccagcggaa cgcgccgccg tggaacggag ggaagcccag gccgtagacc
    28081 agcgccatat ccgcttccgc cggagtggcg ataatgccct cttccagaca gcgcaccact
    28141 tcgttgacca tcgggatcat catgcgggcg ataatctctt cttcgctgaa atcgcgcttc
    28201 ggttggctca cttctgccag caggtcttca acggcggcgt cttcttcttt cttcggctta
    28261 cctttgctgt cttctttata acgccagaaa ccgaggccgt tcttctgacc aaagcggttg
    28321 gcatcaaaca gcgcgtcgat ggcatcgcgg taatctttct gcatccgctg cgggaagcct
    28381 gctgccatga cagcctgagc gtgatgcgcg gtatcaatgc ccacaacgtc cagcagatat
    28441 gccgggccca tcggccagcc aaactgtttt tccatcactt tgtcgatctt gcggaaatcc
    28501 gcgccgtcgc gcagcagctg gctgaaaccg gcgaaatacg ggaacagcac gcggttaaca
    28561 aagaagccgg ggcagtcgtt aaccacaatc ggcgtcttgc ccatcttgct cgcccaggcg
    28621 acaactttcg cgatggtttc gtcggagctt ttctcgccgc gaataatttc taccaacggc
    28681 attcggtgga ccgggttaaa gaagtgcatc ccgcagaagt tttccgggcg ttccagcgcg
    28741 ttggccagtt cgctgatagg aatggttgaa gtgttagacg ccagcacggt atcctggcgt
    28801 actttttgtt cggtttctgc cagtacggct tttttcactt tcgggttttc aacaaccgct
    28861 tctaccacaa tatccacgcg gtcaaatccg gcgtagtcga gcgttgggtg gatcgtggag
    28921 atcacgccag ccagtttcag accatcgatt ttgccgcgct caagctgctt gttcagcagt
    28981 ttcgcggctt cggtcatgcc gagggttaac gacttgtcgt tgatatcttt catgacaacc
    29041 ggcacgcctt tccacgcgga ctggtaagcg ataccgccac ccatgatgcc tgcgcccagc
    29101 acagcagcct gtttcggggt ttcaacgtct ttggtgagtt tcttcgcttt gccttttaca
    29161 tattgatcgt taaggaaaat gccgaccagt gcgcgagctt cgttggtatg cgccagcggg
    29221 acaaaacttt tgttttccag atttaaggct tcttcacgac caaaacgggc cgcagcttca
    29281 atggttttta ctgcggtgat gggggccgga taatgtttcc ccgccgtttg cgcgaccatt
    29341 cctttagcga tggtgaagct catggtggct tcaatcttgc tcagttttag cggttcctgc
    29401 ttcggctgac gtttcgcttt ccagtcgagg tcgccgttaa tggcctggcg taaaatcgcc
    29461 atcgcccctt caatcagttt ttctgctttg actacgccat ccaccagacc gattttcagc
    29521 gcctgatccg cgccgacatc tttaccggcg gcaatgattt ccagtgcact gtcagcgccc
    29581 agcatacgtg gcatacgtac agaaccgcca aagcccggca tgatgcccag tttggtttcc
    29641 ggcagaccga tgcgcagatc cggcgtcgcc agacgataat cggtcgccag cacgcattcg
    29701 cagccaccac ccagcgcata gccgttgacg gcagcaatgg tcggcaccgg cagatcttcc
    29761 aggcgattaa acacgctatt ggcaaagtgc agccactgac ttaactgttc ttcaggaacg
    29821 aggaacaggg acaaaaattc ggtgatatca gcaccgacga taaaggctgc tttgttcgaa
    29881 cgcagcagca gcccttttag atctgattgc tgttccagca cgccgatggc ctcgccgagg
    29941 ctggcgacgg tcgcagtgtc gagtttatta actgaacctg gggcatcaaa taccagttcg
    30001 gcaatgccat cttccagcca gtcaaggtac agggtgtcgc ctttgtaaag catgtcagtc
    30061 tcctgaatcc gcaaggtaat ctggtcgtac cagatgcatc ggagtgtgtg ttttgtgtta
    30121 aaaatatgca aataaaagat taaagaaatg ccgatctgat cacgctcggc agaaatcacg
    30181 ctctggatga acgatgtgct aagatgcgga gacttaaggt caaaaaaaca gaagggtaaa
    30241 aaatggaatc actggcctcg ctctataaaa atcatatagc taccttacaa gaacggactc
    30301 gcgatgcgct ggcgcgcttc aagctggatg cattacttat tcactccggc gagctgttca
    30361 acgtttttct cgacgatcat ccctatccgt ttaaagtgaa cccgcaattc aaagcgtggg
    30421 tgccggtaac tcaggtgcca aactgctggt tgctggtgga tggcgtgaac aagccgaaac
    30481 tgtggttcta tctgccggtt gattactggc acaacgtcga accgctgccg acctccttct
    30541 ggactgaaga tgtggaagtg atcgcgctgc cgaaagccga tggcattggt agcctgctgc
    30601 ctgctgcgcg cggcaatatc ggttatatcg gtccggtgcc ggaacgtgcg ctgcaactgg
    30661 gtattgaggc cagcaacatc aacccgaaag gggtgatcga ctacctgcat tattaccgct
    30721 ccttcaaaac cgagtacgaa ctggcctgta tgcgtgaagc gcagaaaatg gcggtcaatg
    30781 gtcatcgtgc ggcagaagaa gcgttccgtt ctggcatgag cgagttcgat atcaatattg
    30841 cctatctgac cgcgaccggt catcgtgata ccgacgtacc ttacagcaac attgtggcgc
    30901 ttaacgaaca cgctgcggtg ttgcattaca ccaaactgga ccaccaggca ccggaagaga
    30961 tgcgcagctt cctgctggat gccggggccg aatataacgg ctatgcggct gacctgaccc
    31021 gtacctggtc ggcaaaaagc gacaacgact acgcacagct ggtgaaagac gtaaatgatg
    31081 aacaactggc gctgatcgcc accatgaaag caggcgtcag ctatgtggat taccacatcc
    31141 agttccatca gcgcatcgcc aaactgctgc gtaaacatca aatcatcacc gatatgagtg
    31201 aagaagcgat ggtcgaaaac gatctcaccg gaccgtttat gccgcacggt attggccatc
    31261 cgctgggcct gcaggtgcat gacgtcgccg gttttatgca ggatgatagc ggtacacacc
    31321 tcgcggcacc ggcaaaatat ccgtacctgc gctacacccg tattctccag ccgggcatgg
    31381 tgttaaccat cgaaccgggt atctacttca ttgaatcgct tctggcaccg tggcgtgaag
    31441 ggcagttcag caagcacttc aactggcaga aaattgaagc actgaaaccg ttcggcggca
    31501 ttcgtatcga agacaacgtg gtgatccacg aaaacaacgt ggaaaacatg acccgggatc
    31561 tgaaactggc gtgatggaaa gctggttaat tcctgcggca ccggtcacgg tcgttgaaga
    31621 gatcaaaaag agccgcttca taacgatgtt ggcgcatacc gatggcgttg aggcggcgaa
    31681 agcgtttgtt gaatcggtgc gggcagaaca ccccgatgcc cgccaccatt gcgtggcgtg
    31741 ggtggcgggt gcgccggatg attctcaaca gctgggtttc tctgacgacg gggagccggc
    31801 gggaacggca ggtaaaccga tgctcgccca gctaatgggc agcggcgtcg gggaaattac
    31861 cgctgtggta gtgcgctact acggcggcat attgcttggc accggtgggt tagtgaaagc
    31921 gtatggcggc ggcgtgaatc aggcgctgcg ccagctaacg acccaacgca agacgccatt
    31981 aaccgaatat actttgcaat gtgaatatag tcagttaacc ggcattgaag cgttgctggg
    32041 gcagtgtgac ggcaaaatta tcaacagtga ttatcaggca ttcgttctgc tgcgggtggc
    32101 gcttccggcg gcgaaagtgg ctgaattttc cgcaaagctg gcggatttta gccgtggttc
    32161 attgcaattg ttagcgattg aagaataatc cccacttcgt tttgcagaac taaggaagcg
    32221 gcagagatgc attttcgcgc cattacccga atcgttggac tactggtcat cttattttca
    32281 gggaccatga ttatccctgg gctggtagca ctcatctacc gggatggagc gggccgcgct
    32341 tttacccaga ccttttttgt cgccctcgcc attggctcta tgctgtggtg gccgaaccgc
    32401 aaagagaagg gcgaactgaa atcccgtgag gggtttctga tagtggtgct gttttggacc
    32461 gtgctgggta gtgtcggtgc gctccctttt atcttctcgg aaagcccgaa cctcacgatt
    32521 accgatgcgt tttttgaatc tttctctggc ctgaccacca ccggggccac tacgctggtg
    32581 gggctggatt cgctccctca tgccatcctc ttttatcgcc agatgctgca atggtttggc
    32641 gggatgggga tcatcgtgtt agcggttgcg atactgccta tcctcggcgt gggtgggatg
    32701 cagctctatc gcgcagaaat gcccggcccg ctgaaagata acaaaatgcg cccgcgaatt
    32761 gcggaaacgg cgaaaactct gtggttgatt tatgtcttgc tgaccgtcgc ctgtgcgctg
    32821 gcgttgtggt ttgccggaat ggatgccttt gatgccatcg gccatagctt tgcgactatc
    32881 gctattggcg gcttctcgac acatgatgcc agtatcggtt atttcgacag tccgactatt
    32941 aacactatca ttgctatctt cctgctgatc tccggctgta actacggtct gcacttttca
    33001 ctgttaagtg ggcgtagtct gaaggtttat tggcgcgatc cggaatttcg catgtttatc
    33061 ggcgtacagt ttacgctggt ggttatttgt acactcgtac tgtggtttca taatgtctac
    33121 agttcggcgc tgatgacaat taaccaggcg tttttccagg tggtatcgat ggcgacaacc
    33181 gccgggttta cgactgacag cattgcccgc tggccgctct ttttgccggt actgctttta
    33241 tgttcagctt ttatcggcgg ttgcgccggg tcaacgggcg gtggcctgaa agtgatccgc
    33301 atcctgctgc tgtttaagca ggggaaccgt gagctgaaac gactggtgca tccgaacgcc
    33361 gtctatagca ttaagctggg gaatcgcgca ctgccggaac gtatcctcga agccgtttgg
    33421 ggatttttct ccgcctatgc attggtgttt attgtcagta tgctggcgat tatcgccacg
    33481 ggcgtggatg atttttctgc ctttgcctca gttgttgcga cattgaataa cctggggcca
    33541 gggcttggcg tggttgctga taactttacc agtatgaacc cggtggctaa atggatcctg
    33601 attgccaaca tgctgtttgg tcgtctcgag gtctttacat tactggtgct ctttaccccg
    33661 actttctggc gtgaatgatg gagtaatacg tgaaaacatt aattcttttc tcaacaaggg
    33721 acggacaaac gcgcgagatt gcctcctacc tggcttcgga actgaaagaa ctggggatcc
    33781 agacggatgt cgccaatgtg caccgcattg aagaaccaca atgggaaaac tatgaccgtg
    33841 tggtcattgg tgcttctatt cgctatggtc actaccactc tgcgttccag gaatttgtca
    33901 aaaaacatgc gacgcgactg aattcgatgc cgagcgcctt ttattccgta aacctggtgg
    33961 cgcgcaaacc ggagaagcgt actccacaga ccaacagcta cgcgcggaag tttctgatga
    34021 actcgcaatg gcgtcccgat cgctgcgcgg tcattgccgg ggcgctgcgt tacccacgtt
    34081 atcgctggta cgaccgtttt atgctcaagc tgattatgaa gatgtcaggc ggtgaaacgg
    34141 atacgcgcaa agaagttgtc tataccgatt gggagcaggt ggcgaatttc gcccgagaaa
    34201 tcgcccattt aaccgacaaa ccgacgctga aataagcata aagaataaaa aatgcgcggt
    34261 cagaaaatta ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc
    34321 actgacacgg aacaacggca aacacgcagc cgggtcagcg gggttctcct gagaattccg
    34381 acagagaaag caaaaataaa tgcttgactt tgtagcggga aagcgtatta tgaacacccc
    34441 gcgc
//
LOCUS       DAJTSF010000037        34306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000037 DAJTSF010000000
VERSION     DAJTSF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.037"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..804)
                     /gene="istA"
                     /locus_tag="OO335_001858"
     CDS             complement(<1..804)
                     /gene="istA"
                     /locus_tag="OO335_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR6002268.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHG"
     gene            1420..1557
                     /locus_tag="OO335_001859"
     CDS             1420..1557
                     /locus_tag="OO335_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002269.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            2157..2936
                     /gene="ipaJ"
                     /locus_tag="OO335_001860"
     CDS             2157..2936
                     /gene="ipaJ"
                     /locus_tag="OO335_001860"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR6002270.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3883..4812)
                     /gene="virB"
                     /locus_tag="OO335_001861"
     CDS             complement(3883..4812)
                     /gene="virB"
                     /locus_tag="OO335_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR6002271.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(5029..5265)
                     /locus_tag="OO335_001862"
     CDS             complement(5029..5265)
                     /locus_tag="OO335_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6002272.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(5269..7170)
                     /gene="ipaA"
                     /locus_tag="OO335_001863"
     CDS             complement(5269..7170)
                     /gene="ipaA"
                     /locus_tag="OO335_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR6002273.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(7179..8177)
                     /gene="ipaD"
                     /locus_tag="OO335_001864"
     CDS             complement(7179..8177)
                     /gene="ipaD"
                     /locus_tag="OO335_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR6002274.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(8228..9319)
                     /gene="ipaC"
                     /locus_tag="OO335_001865"
     CDS             complement(8228..9319)
                     /gene="ipaC"
                     /locus_tag="OO335_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR6002275.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(9339..11081)
                     /gene="ipaB"
                     /locus_tag="OO335_001866"
     CDS             complement(9339..11081)
                     /gene="ipaB"
                     /locus_tag="OO335_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR6002276.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(11087..11554)
                     /gene="ipgC"
                     /locus_tag="OO335_001867"
     CDS             complement(11087..11554)
                     /gene="ipgC"
                     /locus_tag="OO335_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR6002277.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(11611..12237)
                     /gene="ipgB1"
                     /locus_tag="OO335_001868"
     CDS             complement(11611..12237)
                     /gene="ipgB1"
                     /locus_tag="OO335_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR6002278.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(12253..12642)
                     /gene="ipgA"
                     /locus_tag="OO335_001869"
     CDS             complement(12253..12642)
                     /gene="ipgA"
                     /locus_tag="OO335_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR6002279.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(12655..14139)
                     /gene="icsB"
                     /locus_tag="OO335_001870"
     CDS             complement(12655..14139)
                     /gene="icsB"
                     /locus_tag="OO335_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR6002280.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            14453..16069
                     /locus_tag="OO335_001871"
     CDS             14453..16069
                     /locus_tag="OO335_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR6002281.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            16079..16441
                     /gene="ipgE"
                     /locus_tag="OO335_001872"
     CDS             16079..16441
                     /gene="ipgE"
                     /locus_tag="OO335_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR6002282.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            16441..16899
                     /gene="ipgF"
                     /locus_tag="OO335_001873"
     CDS             16441..16899
                     /gene="ipgF"
                     /locus_tag="OO335_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR6002283.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16908..18023
                     /gene="mxiG"
                     /locus_tag="OO335_001874"
     CDS             16908..18023
                     /gene="mxiG"
                     /locus_tag="OO335_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR6002284.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            18031..18282
                     /gene="mxiH"
                     /locus_tag="OO335_001875"
     CDS             18031..18282
                     /gene="mxiH"
                     /locus_tag="OO335_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR6002285.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            18295..18588
                     /gene="mxiI"
                     /locus_tag="OO335_001876"
     CDS             18295..18588
                     /gene="mxiI"
                     /locus_tag="OO335_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR6002286.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            18594..19319
                     /gene="mxiJ"
                     /locus_tag="OO335_001877"
     CDS             18594..19319
                     /gene="mxiJ"
                     /locus_tag="OO335_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR6002287.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            19316..19843
                     /locus_tag="OO335_001878"
     CDS             19316..19843
                     /locus_tag="OO335_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR6002288.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            19785..20480
                     /gene="mxiN"
                     /locus_tag="OO335_001879"
     CDS             19785..20480
                     /gene="mxiN"
                     /locus_tag="OO335_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR6002289.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            20473..20880
                     /gene="mxiM"
                     /locus_tag="OO335_001880"
     CDS             20473..20880
                     /gene="mxiM"
                     /locus_tag="OO335_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR6002290.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20864..21292
                     /gene="mxiM"
                     /locus_tag="OO335_001881"
     CDS             20864..21292
                     /gene="mxiM"
                     /locus_tag="OO335_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR6002291.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            21422..22054
                     /gene="mxiE"
                     /locus_tag="OO335_001882"
     CDS             21422..22054
                     /gene="mxiE"
                     /locus_tag="OO335_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR6002292.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            22041..23741
                     /gene="mxiD"
                     /locus_tag="OO335_001883"
     CDS             22041..23741
                     /gene="mxiD"
                     /locus_tag="OO335_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR6002293.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            23760..24827
                     /gene="mxiC"
                     /locus_tag="OO335_001884"
     CDS             23760..24827
                     /gene="mxiC"
                     /locus_tag="OO335_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR6002294.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24840..26900
                     /gene="mxiA"
                     /locus_tag="OO335_001885"
                     /gene_synonym="virH"
     CDS             24840..26900
                     /gene="mxiA"
                     /locus_tag="OO335_001885"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR6002295.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26913..27314
                     /gene="spa15"
                     /locus_tag="OO335_001886"
                     /gene_synonym="spaK"
     CDS             26913..27314
                     /gene="spa15"
                     /locus_tag="OO335_001886"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR6002296.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            27318..28610
                     /gene="spaL"
                     /locus_tag="OO335_001887"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             27318..28610
                     /gene="spaL"
                     /locus_tag="OO335_001887"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR6002297.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            28703..29041
                     /gene="spa13"
                     /locus_tag="OO335_001888"
                     /gene_synonym="spaM"
     CDS             28703..29041
                     /gene="spa13"
                     /locus_tag="OO335_001888"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR6002298.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            29028..29906
                     /gene="spa32"
                     /locus_tag="OO335_001889"
     CDS             29028..29906
                     /gene="spa32"
                     /locus_tag="OO335_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR6002299.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29900..30781
                     /gene="spa33"
                     /locus_tag="OO335_001890"
                     /gene_synonym="spaO"
     CDS             29900..30781
                     /gene="spa33"
                     /locus_tag="OO335_001890"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR6002300.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            30786..31436
                     /gene="spa24"
                     /locus_tag="OO335_001891"
                     /gene_synonym="spaP"
     CDS             30786..31436
                     /gene="spa24"
                     /locus_tag="OO335_001891"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR6002301.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            31451..31711
                     /gene="spaQ"
                     /locus_tag="OO335_001892"
     CDS             31451..31711
                     /gene="spaQ"
                     /locus_tag="OO335_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR6002302.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            31711..32481
                     /gene="spa29"
                     /locus_tag="OO335_001893"
                     /gene_synonym="spaR"
     CDS             31711..32481
                     /gene="spa29"
                     /locus_tag="OO335_001893"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR6002303.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            32488..33516
                     /gene="spa40"
                     /locus_tag="OO335_001894"
                     /gene_synonym="spaS"
     CDS             32488..33516
                     /gene="spa40"
                     /locus_tag="OO335_001894"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR6002304.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            33529..33771
                     /locus_tag="OO335_001895"
     CDS             33529..33771
                     /locus_tag="OO335_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002305.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10647 a         5069 c         6922 g        11668 t
ORIGIN      
        1 accgtggcgg ttggctgttt caacatcgac agtgctcccc atcgggcgca ggcgagtcat
       61 tagtgggatg taaaaactgt tacgggtgta ctgcaccatc cgttccacct tacctttagt
      121 ctgtgccctg aaggggcgac acagtcgggg agagaagccc atctccttgc cgaactgcca
      181 cagcgaagga tggaaccggt gctgaccggt ctgatatgcg tcacgttgca gaaccacagt
      241 tttcatattg tcatacaaca cttcgcgcgg cacaccacca aagaagcgga acgcattacg
      301 atggcaggtc tccagcgtgt cataacgcat attgtcagtg aattcgatgt acaacattcg
      361 gctgtatccg ggaacagcaa cgaacacgtg aagcggtgag cgaccattac gcatagtgcc
      421 ccagtcaacc tgcatctgtc gtccgggttc agtttcgaac cgaacggcag gctcctgctc
      481 ctgaggaacc gagagagaac gaatgaatgc cctgagaatg gtcattccgc cacgatatcc
      541 ctggtctctg atctcacgag cgattaccgt tgccgggatt ttgtaaggat gagcatcggc
      601 gatgcgttga cgaatataat cccggtattc atccaggagt gaagcaacag caggtcgcgg
      661 cgtatatttt ggcggctcag attttgcctg caaataacgt ttaacggtat tgcgggagag
      721 ccccagttct ctggcaatcg tccggctact cattccctgc ttgtgcagga ttttaatttc
      781 cataactgtc tcaaaagtga ccataagctc tcctgaatca ggagagcaga ttaccccctg
      841 gatctgattt caggcgttgg gtgtggatca ctattgcacc gttcgtgaca cttgttgact
      901 acctgtgcgg catctggtat caaggtggcg tgaagccggt tcaggttctc atgcggaacg
      961 ttggaaccta tatccccccg ataccaaagg cgaaatgtaa gtgacaaaat gcaatgaaca
     1021 cgttctccgt taactgagga gcatggtgat aaataaaggt aaatgcagaa ggtgtagagt
     1081 ctcgcagatt tctgacatca tagagcctaa cattagatgc ataacgctac agattgtaga
     1141 atctctacag gtgaaatata cactggttgg cgtttatgtc gcaaaacatg tcattcaggt
     1201 tcatctcacc aataaggata tgagtgaagt ggaggataag tgagtctgct gtcagagttt
     1261 tctggtgtat gtcagtaact gcgaaccatg tctgcttgat atgggagctt atggaggctc
     1321 tctgtacaag gttagggagc taacaaagca gggataaaaa gtccgcctga tgcaggcacg
     1381 gttcgttaaa tcttttgcca ttggtaacaa aatggtgcga tgaatgttca ggctatcttt
     1441 atcttgatgg tggttcagct gtttggtaaa gaaatcgctg taacaataga agaactgcag
     1501 gcagtactgg tcccacatcg tttgctcagt caactggtga agttccgtag cgcgtaaatt
     1561 aatgctttgc atgaaacgtt actggagtgg atgaaagaat acctgcatat atcattactg
     1621 cgttggatta ccagtacaac agattgaatg aattggacat gttgatagac ggcattgaaa
     1681 agcagcttat aagcatagtg cggcagagtt aattggagag cacttcgcct tctgagtatt
     1741 aagtaagcat ggtggtacct gcggatacta tctattcact gtgctcggtc ggcagcctta
     1801 ctgaccaagg agttggggga gttgataaca aagttgaaaa aatagctctc aaccaaggtg
     1861 gttattgtaa caatagcaaa taagctggtg tgacctgtat cacaataacc gattatggac
     1921 gtaaatacga caaaaatatg tcagtatcag gcggtattaa ttgctgatgc tgatggacat
     1981 taactcttaa taaaagggtg gatgctgaaa ggttgttatg gtgacggacg tgatgacgac
     2041 ggaaaagagt atgaggtttt ggatttaaag ttcgttcggg gaataaggac ccggtcagct
     2101 aattctcata gtagttcgca ttgctggatg caatactgtg ctgcaattac tcgtcgatgt
     2161 cggaacaacg gaagccttgc aaacggggat gtattcatac aggtgttatg ttatatgggg
     2221 ttttattgca gggggctata ccaagagaat atatgatatc tcatcagact gatgtgaggg
     2281 taaatgagaa tagagtaaac gaacaaggtt gttttttggc gaggaagcag atgtatgaca
     2341 attcttgtgg agctgccagt ttactctgtg ctgcaaagga attaggggtt gataaaatac
     2401 cacaatacaa agggtctatg tccgaaatga caaggaagag ttctcttgat ttggataatc
     2461 gttgtgagcg ggatttatac ttaataacta gtggtaatta taatccgaga atacacaaag
     2521 ataatatcgc ggatgccggt tattctatgc ctgataagat agtcatggct acgaggcttc
     2581 ttggattgaa tgcttatgtc gttgaagaaa gtaacatttt ttcgcaagta attagtttta
     2641 tttatcccga tgcaagggac ttattaattg gcatgggatg taatatagta catcaacgag
     2701 atgtgttatc ttctaaccaa agagttttgg aagctgttgc tgtatccttt attggagttc
     2761 ctgtcggcct ccactgggtg ttgtgtagac ctgatggaag ctatatggat ccagctgtag
     2821 gagaaaatta ttcttgtttt tcaactatgg aattaggtgc aagaagaagt aattctaact
     2881 ttataggata tactaaaata ggtatttcca tagttataac taatgaggct ttgtaaattg
     2941 tcgtttttta tctgtgaagg agcttgaata ttttggggtg gggggcataa caaatgatat
     3001 tactgagtac aaatttagct atattgggcg gattagttgt gtgggaagac gaaatgtttc
     3061 tcttgaaata agtgaactcc ctcaaggaat gattgtgtgc ttaaatattt aagtgctggt
     3121 aagtttgaga aggctgacat attttgcaga gcaaacagtg attgacttgg atgatatttt
     3181 tattttagtc actcgttgta attggagtat gctttatttt atttgaaaat agatgtattt
     3241 tatacccgca atttaaactg taagccctat gaggggtatg gatattataa gttactcttt
     3301 gaccaagcat aacttttggg gttagaaaaa taaatctctt gttaataatt aatatgattt
     3361 taaaatcaaa cttgtttata attataaaat aatgtaagtc ggtcatttta tgggaagatt
     3421 aggagaataa aacagtaatt atatttgaga aatctgctaa tatattctta aagctttttt
     3481 gatactgtga acgaagtctg ttaatggtgg attttttgtt tatcaccatg taaaaaaaat
     3541 tgagtagtaa aagcgttgac tgctaatcct cgctgttttt attgaaacga ttaaaaatac
     3601 gctgaatatt ttcggccagt cactcatatt cacataaaat tttgaccatc atgccgcatc
     3661 ccttagttgc acaattcatt ttcaatgata tgaatattac taatttcacc agataatttt
     3721 ctcgtggtca gcagggtatg agttttcacc gtaattttaa ggaaacacag tgcgtgggga
     3781 ttgaataaca gatagccgac ggaattaccc caccggctga aagtcaagtt caacgaagat
     3841 ttttattatc tgaattgggc agtttacatc agtgttcgat gtttatgaag acgatagatg
     3901 gcgagaaatt atatcccgaa tagcttcatc tatttctctc tgaactgttt tgtttatttt
     3961 agataatgta aatattagag ttctgtttgt tttattctct tttcttttta tgtaagtccg
     4021 tttgtcttta gaaataaata acgagtcaac ttttagcgaa gggttttgct ttctgttttt
     4081 gcttttcttt atgatgttta aaatttcttt tttatatatg tccgggggca aatttgtatc
     4141 aaggtctttt atttcttcct ttaatattgg tagtgtagaa ctaagagatt cattagcctt
     4201 ttcaagtcct ttataataat tgaataatat cttgtagtca ttaaagttaa gttctgacgc
     4261 gattggaaat agagatatta tttcctgtgg aacgcttgct gcctgaaagg cacgagtgac
     4321 tttcgcgcga gacagattct cttttttggc tatgtcttta taggacatcc ctgatacttt
     4381 cagaaaatta agaccaatac caagttctcg gatgctatgc tcttttgctg tttgtatatc
     4441 gtttgctagt tttctggcat caagagtaga tatatattct tttgaatata gaacttcaag
     4501 atctgctcct gcatatattg cagatgctct tctacgagtg ccatccagaa tttcaattct
     4561 accatcaatc tcccttccta ttacagggaa gaattgttgt agctttatag tttttatgat
     4621 atcggctaat gattcttctg ttagggattc ttgatcgcgt acattcgttt tttggttgac
     4681 gaaggttaaa tcttggatcc ttttatgagg aatcttggct ttgataaagg aaactttatt
     4741 accagaatgg agtgtaaact ctttcttttg gccttccttt atacttaaca agtcgttgca
     4801 caaatccacc atatcacacc ctgtttattc atattgattt atattgacaa aagttaaatg
     4861 cagtgtgagc aagattgtat gtaactgttg atgatagtca acggtagtct taaaaattac
     4921 tattttaata gctggattga tgcaatttat ctttattgga taaaagaata atattcttgt
     4981 ggagctctga tgtgagagct ccatagatga aaaaatgctt atcaattact atattgattt
     5041 tgtactattt ttgactacag aaataagatc tccaatagta ataatgtgtt ctaatgtaga
     5101 ttcatctata cgtagattga actcaaaact aatcatatcc agcatttcaa tcagagaaag
     5161 agaatcggca gaaagatctt caataaattt agtttcttca gatagtttgc tgtacgctat
     5221 accatagcaa aaggccacta ttgataatat tttttctttt atcatatttt aatccttatt
     5281 gatattcttt aatacttttg atagggaagt ggttacatct tttgctgctt tatatatatt
     5341 attgttttca gctgtaatat cattgttatt ttttaaatca gatatatcat ctgtaagtaa
     5401 ttctgcagag gtatcgtcaa tttttgacaa taccttgctg agggcgctag atacttcttt
     5461 tgccttttca tagatcgcat gatttttatc aattgtatct gcgttggttt ctgttacgta
     5521 gtttcttata tttgcatctg taacttttaa tttgttgttt attctatgtt ctgttgtgga
     5581 ttttgtaatg tcctttgatt tttcctgtaa ctctcgttct tgtgttcctg atttagttcc
     5641 aatgagagat ataagattac ttagggagtt tgttactttt tttgaagctt caaatatcgt
     5701 ttcccgtgtt aaatatgtgt ctccatttct tgataaaaaa tcagtctgaa gtacttttga
     5761 atcagtgttt gagttgaatt tattgatttg cgaagctact ctatgagaaa gagaagcgct
     5821 acttttagtt gtatctgatg agttatcatt actgcttgtc actccttccg ggtggagagg
     5881 gggggtggtt gaagagttct gtatgttcag aggcctgtgt ccccgagaaa gagagttatt
     5941 tgcagatctt tcttgactac tcgtagttaa caggtctgaa cctattccat ccgaattatc
     6001 tggagtatgg ttgaaagcct tttctaaagc tgaattattt gcaaatgtct ggacagtatc
     6061 tgatatagag tgtggtagaa cggaagttac tttttgaact agtgctcttt gtggatttag
     6121 taatgagttt ccgttacggg ataatagatc gtttgtctga gaagtaggag actgtgcatc
     6181 attttctggg ttctcgtgat agctattgtc atatactctg ttgtcaaaaa ctctattatc
     6241 gtaagttcta ttatcattat ttatatgata gtgaattaca ggttgggatg ttttctcatt
     6301 ggctccgggg gttggtccgc catctggcat atcttcggga ggtgtaggga ggccatcttg
     6361 gataggatgc gaatctttta tatcttctaa aatatcatta aggacttttc tcaacagctc
     6421 aggaaacacg gctactgtat ttaagtctct tgacagttta cctataccaa atcctttact
     6481 atcaatagta tttttacgtt gattgcgcaa actatcaaag gcaagttttg cctgtgcgtc
     6541 aactaaatca tctagtttga gagatgaaac agtttcatta cttaacttta attcagcaag
     6601 aatattattc ttgataaatg atgatacttc agtattgagg agtttttgtc ccaatgataa
     6661 agcttcacca gatgtgtgtt gttgtgctaa tagtgcaagt ctgtgctcaa tgttttgtcg
     6721 taattgatgg agtttcttat tctttgccga ccatggtcca aaggcagaat cttgggataa
     6781 tttatctttt ataattagtt ccgaatactg tgcgacaatt ttttgaagtc ccttttcgtt
     6841 ttcaaaatta agaccatttt gatgtactaa ggagtacaaa acatttgaaa attcgagaat
     6901 ctcgcttttg gcatacgcat tcattgagtt ggaataaatg cttcttaaca catcttcttt
     6961 ccctgaggaa gaaaataaaa aatcaagaca tttctgatta aaagacgttg caaagttttc
     7021 tttttcagac actgatgctg gtgtttttag agaagtttgc gaactatgga tatcggatat
     7081 tttgcttttt aactcgctgt attctgttga tgatggtgaa gttgccttat ataagaatgt
     7141 tggcgcttga gtattattta cattatgcat ggcgcacctc agaaatggag aaaaagttta
     7201 tctgtatctg tacatgagct tattgtacta ctcaaaacct ttactaaatt atcaaaaata
     7261 ctattggcat tactgtattt ttgaactaaa gtttgaagat tatttttcat tgtttcatct
     7321 tcggcagaga atccggcatt ccatgcctga tattttgcat tatctagcac aacctcgcca
     7381 tttccaccta gattatctaa gctttttaac atattgtcta ttggggtcat gtttatactg
     7441 acaacatatc ccccgttttt ttgagatacc ttgccgattg ttccacctaa ttctgtaagc
     7501 catttatttg cttgttcctg actaacagta ttatttgctg gatatagcgg tttatcttta
     7561 tatttttcct tgagttcttc caatgccttt ttaagcgagt tgacttgtaa tttcacggag
     7621 tttccgtcgt tacctccggg agagatccag ccggcaagac tggaaagaac agcgctaaaa
     7681 tcttgataca tttgagtata tgaactaacg gcatgttcat atactttcag atactgttca
     7741 ttaatatcat tgatggagtt tgcaatttta gcccacagtt ctctatgaga tatcatttga
     7801 tctccatcaa gctcggcttc tttcggagct gaatgtaata attctcttgc gtctttatta
     7861 attggatatt cgtgcctgga aagaatatct aatagttgag cggatttatt tacatccatg
     7921 ctaatctgag atgaatgtaa tgctatttct tctagcgatg ttttagtcaa cgttttttgt
     7981 gaaagctctt tctttaatgc ctgatttgtt gttctgatat tatgaagggt gtcgttgagc
     8041 atagtaaggg aacttacagg atgagaactg gtcgttgttt ttatatcaga attaactgtt
     8101 tcggttgatg aaccgttggt attgtttgga ctgaatgatg aggtggaaat actattagtc
     8161 agagttgtta tattcatggt tatttcctta caggaagagc catatattat tatggctctt
     8221 ggatatatta agctcgaatg ttaccagcaa tctgactggc tgtcgaattc tttgattggt
     8281 tgatgctgtc aattatattc aataattttt gtattaattg atttgtcgct tgggatgctt
     8341 ctttagatac ttgggatgct tcctctgctt gtttactgct ggcctgactg attagttgtt
     8401 cttcttcttc aagagcagat gcataacgcc ctcctgatgt ggatatatta ccagcaacgg
     8461 ctgatgtttc cattgttgca cggccaatgt tttgtttttg ctgcgcagaa agggtattga
     8521 gctcgtaagt tcttctctgg gtgtcaattt tatcctgcaa agaaatatcg ggagaactaa
     8581 gagaagtttt atgttcagtt gacagggata tattatctgg cgccagttta tttttaccta
     8641 aaaattttgt gctagagtta gtttgtggtg aggtgatatt tgtatctatt tgtttattta
     8701 accctaattt agaacctgca agctcttttt caagagattg agcagtggca aggttctttc
     8761 ttaaggctcc tttttggtcg ctaatccctg aatgcgtttt tttggcaccg atacccgtta
     8821 tacctacttg tgtgactgct ccagtaatgc ttgatgatag ggctgccagg ccttgccgaa
     8881 caatgttctc tgcagctgat tttgtagcat cgaacgcaat caatgacaat tgagagccca
     8941 atttagtttc tgcagtgcgg agagcagaaa gtagaacggc tacactaata attaaagaaa
     9001 cagcattaga agaaagactg gaaatatcca gagtgttctc tggagtaagg gtgtgactca
     9061 tctctgaaag atctttcatc tttttgtcag taaggcgagc aatgatttct gaatcatgct
     9121 gaacctgctc tgataacttt aaaagttgat catcatttaa tgtggttgtt aatacgggat
     9181 ttttaccgac attaagtgga atatgcgctg caatctgctg ataattttgt gatttttgtg
     9241 tttcggaaga actttgagtt tgttttgtgg atatatctgt atataaaatc tgggttggtt
     9301 ttgtgttttg aatttccata acattctcct tatttgtatc aagcagtagt ttgttgcaaa
     9361 attgcttttg caacatcagt tctattagcc tgagagttgg acattgaggc taatagatct
     9421 gcaattactt cctgcaattg gccgaatttt tctattgctt cactgatata ttttgacagt
     9481 tgttcaactt gatatttcga taatgtcagg tctgctagat ttgtcgacgc gctgttctgg
     9541 aaaacagcag aagcgacact tcctcccgct tgtgtggcag agttaacact ttctcctaat
     9601 aaaactgctt ggtttaaatg ggtggaaata atttgtttgg atattacttc atcacccgct
     9661 gcaccaagaa atttatttaa tctggcaaca gcattagtga ttaaatcgtc cagttgagaa
     9721 gaaaaattct tgagaaactt tggtataagg tctgtgaggg ttttacctat tattttgcca
     9781 atattttctg caagttttgc tgctgcctgt ttaccaacag tggctacgag aacgactgct
     9841 gcaactagga caagagcgcc tgcgattgcc cccagaatag agccaatcat tttggctttt
     9901 ttcgagtcga cgcccaagcc ttcgagcatt tttgtaaatg catctgaaag gagtttgatt
     9961 aagggttcaa tgactgcttt catgatcgga ttcagggctt gttccatgaa ggaattgccg
    10021 gtcgctgctt gtactatagc atccgtaacc ataagagcta aaccaacagc tgccagtgct
    10081 agagaggctc ctccagaaaa agctgctgca acaacactaa cgatagttaa aagtgcccca
    10141 agtattttcc caacacaacc cattactctg ttgagttctt ctgctttacg tacttcagca
    10201 gcatactcat cagattttct ctccatttca gtttttcttg attcttggag agactggaat
    10261 agagccagat cattttttaa agattcttca ttattttttc caactagttg aataaaggtt
    10321 gccatcaatt gagtaacact ggcaagtccg gttaatgatt tctgctgggt tgatagctgt
    10381 tcagcagatg ctgtgtttga aaatgcagaa aaagagtcta tttctttttc gagttgcatt
    10441 gatttatctg taagtttgct atgaattgac agggttttct gctcaatcaa tgtcctgtct
    10501 ttaactgctg cgtctttttt gatagtgagt tgtatttctt cccggcttaa ttttttcttt
    10561 tctggtgact ctgggtcgag ttcggataat cttgtttgaa tttggttaat tttattttct
    10621 aggtctttta ttttagaatc tgcgtttttt agtttattaa tttgtttttc atagtctctg
    10681 gttagtcctt cagtttcaga tagaagagtg ttaattttat cggagaattc taggtttttt
    10741 tgctgtcttg cctgttgctg ggacttccaa gcagtaattt tatttgttaa tgcagttaaa
    10801 gatttttcac cgagtatttg aataaggttt ccaattaaaa gcgttagttg ggaacttgca
    10861 tttaatgact ttggtgcttt aagttcaggg attaatatat ttgaagttga gtgtgcatta
    10921 gttgtattaa tattttggtt agcagtaaga tcagcaattg taagagaaaa taatttgtta
    10981 gctgcatcat ttgcagcttg gatagtattg tctccaagct cagtggaagc caatattttg
    11041 gcaagaggaa aaccagtggt tgtggtgctt acattatgca taataattac tccttgatat
    11101 cctgaattgc gtccaagtat gattgtgctt ttatttttaa tttttcatca ttgctgtgtt
    11161 gaattacgag ttcgaagcac tctttagctt ttaagggggc tttcaaccga agctggcatt
    11221 gtccagtatg gaatactggt gtatagtcat tttttcctaa tgcaaaagcg acagcataaa
    11281 ggtctgctgc ttgttggaac tgttctttta tctgataaat agctgcgagt cccataatgt
    11341 agtctacatt gtaaaagtcg tatatacata aaaacctgaa gaaaacttca gcttcctcta
    11401 ttcttccttt gttgtaaaag tcataagcat atgaataaat gtcatccatc atatcatcag
    11461 gaattgcatt aatatctttc agtgtagcgc cagagttaat tgcatcaatt actgcagtag
    11521 agatgctttc attttcggtg atatttaaag acataaggtc tcctttttgt ctgctttact
    11581 ttaataagta taagatttaa tataaaagat ttaatttgta ttgctttgac ggtatacagc
    11641 ctttagaacc tctgcgctta tttctatttg taaaactttt gttgattcat taaatatttt
    11701 atcgtagacg gaactggata ctagatttgt aagatccttc atttgtgtgt taaacaaatc
    11761 catgtcactg ctatagcgtt ttaaaatctt tgctctgagg ccagatgagg caatctcgtt
    11821 tagtgctgta tgactgatag atgtttgtgc tgatttttca tcaagtttta tatccgggaa
    11881 ttttttttca atagctgcaa atatgttttt tttgttatct gaggtcaaaa caactccatt
    11941 gtgtatggcc atttcatcta tacttctgtt tatcattgaa gagacataag tggttcgctc
    12001 ttggctcatc caacaaaata gttgattccc cgaattacta tcttttattt tgtctatgac
    12061 gctgtccctg attttattta tattcaccgg gcatccaaaa ttcttctgag gaaagcgttg
    12121 ttttaagtta aaaacagata atattttctt tacaagctta ttgcgactta acgaatcaaa
    12181 tgatgggcga ggaatcgcga attcaacctg tggaagtatt ttgtttagaa tttgcatgat
    12241 accccctata tgttagttca cttctgaagt gatgtttgca atggttagtt taatacaaaa
    12301 taaaaattca tacaaagatt caatgagttc atcaggtgaa agatattttt tgtagtgttt
    12361 cttggtcaaa acacattgat gagtatcagg gagaagacag atagaagaac cataatctct
    12421 actcaaagag tagttaagga tgagtatgtg ttctggatta ataatatttt ctgtaaaaag
    12481 ttcatgttca ttaagaagtc cggtcaaaaa gatgtattca tcattatctg ttatacaaac
    12541 aggtatttgt tcttcgagca atataaaggc ttgattttta tcgttaaaat caagctttgc
    12601 acctgttatt tcataaagtt tatcatatag tttgcgacac atttctatgg ccttctatat
    12661 attagaatga gagttattca ataaaagctt attgatctgt aataagtcgt gaatgtaaag
    12721 tttggttcca agatctggcg atttaagaga attgtaataa tcaataattt ttattgattt
    12781 ttcgatgatt tcttcttttt tatctgttgc ttcggttttt aattcgttta atacacgagt
    12841 catattgctg ggtgctgatt ttactaagta tgagtggcaa gcgttgatga ccgcatcaaa
    12901 gtcttctgaa gatacatttt cagtaggatg tttgtaaagt gaaattgata aaggcttgaa
    12961 tgtttttggt gtgtggataa ggttaatttt attgaaactt ttaagatagc tgcgtcgtaa
    13021 ttgagtcaat ctggaaagat caaaatcaga ttcaatatac ttcattattt cgttataaga
    13081 ctgattgaga ctttcaatgc gttgcctaac tttatttgca tacgcgtata catcgttagg
    13141 tgtagcaacc aatttaacgt cagggagtgg aaagtatatt tcagaatttc ctgctttaag
    13201 aacattaagt gccataccag cacagttttc agatttactg attaatttat aatttgccaa
    13261 ttgtctgaaa gcatctgctt tctttttgca tataaatttt gacatgtttg tttcatcaag
    13321 gccaaaaagg ttatggctga ttttaccatc ctttgtttta ttatcacctg ataatggaat
    13381 gtatacttta tcagcactga ccccccaata accatagggg tctttttttt gattagctct
    13441 gggaaaaaat atttttttgt tgttattttg atcttgtttt gaatttaata agtttctaac
    13501 ctcttggcct gcatcaagtc tttcggctgt ttggtctgaa atcatattta gtttgtcttg
    13561 cttataggta tcgacactgt aacctgagcg tttttcaaaa aggtagttta tttctgtatc
    13621 ttttgtaaca ttcccgccgg gataccagct gagataggta tgagttggtt cctgtagtaa
    13681 attgtttttt atactgatac tagtatgccc taaatgggtt atgcctctat ttccccagca
    13741 acgaaaaact aaatgtcttt caagattttt ggctgtaccg atgccatgaa aacgctctgc
    13801 ggtattcaac attaatttga tgtctctacc tgtgaaagag tgttgaattg agtttttagc
    13861 tgcttcatag gcggcaaacg cactttctgc agatttttcg ctagctaatg tattgaggaa
    13921 aagcaataag gatttttgat tctctagtcg ataatttttt agttgttcat ttaatatctg
    13981 tgaatttatc tttgcattaa tagtgcttaa tgttctgaaa aagagatcct ttaggttgaa
    14041 tttctgtgct atcaatatag gtccatgctc tgtatcttca acgcgtatag gcccttttgt
    14101 attgcttgcg tcaatgaaat tgctaatttt gaggatcata ctttattaac tctccattac
    14161 ttggtgattg tggggatgta aaatgtattt ggtatataat atattcaatt aataaattta
    14221 gaaacttgag cctgttaaca taatcaaatt ttcttttgct gtacataata tgtacctcgt
    14281 gagcatatgt agtgctcgtt tcatcatgaa atcccacaag ataaagtgcc tgatgtatca
    14341 ggctcggagt gttatagaaa aagagagaac cctgttgaat aagtatcttt tttgttgtaa
    14401 aacgaggtac aaaactgcaa tcaatgcccc atttatgact gaggataatt aaatgcacat
    14461 aactaatttg ggattgcatc aggtttcatt tcaaagcgga gattcctata aaggcgcaga
    14521 agaaaccgga aagcacaaag gtgtaagcgt gatttcatat caaagagtta aaaatggaga
    14581 aagaaataag gggatcgaag cgcttaatcg attatattta cagaaccaaa caagcttaac
    14641 aggaaaaagc cttttgtttg cgcgtgacag agctgaagtt ttttgcgagg caataaagtt
    14701 ggcaggtggt gatacgtcaa aaattaaagc catgatggaa cgattagata cctataaact
    14761 tggtgaagtt aataaaagac atattaatga gcttaataaa gtaataagtg aagagataag
    14821 agcacagcta ggcattaaaa ataaaaagga attgcaaacg aaaataaaac aaatatttac
    14881 tgattattta aataataaga attggggacc ggtcgacaag aatattagtc atcatgggaa
    14941 aaattatggt tttcaattaa ctcctgcctc tcatatgaag atagggaata aaaatatatt
    15001 tgtcaaagag tataatggaa aaggaatttg ttgtgcttct acgagagaga gtgatcatat
    15061 cgcgaatatg tggctttcaa aagtggtgga tgatgagggg aaggaaattt tttcgggtat
    15121 cagacatgga gttatatcag cctatggatt gaaaaaaaac tcatctgaaa gagctgttgc
    15181 tgctcgtaat aaagctgagg agttagtaag cgcagcatta tatagcagac cagagttatt
    15241 atcacaggct ttgtctggta aaacagtaga tttaaagatt gtttcgactt ctcttctgac
    15301 gccgaccagt ttaaccgggg gagaggagag tatgttaaaa gatcaggtga acgcattaaa
    15361 agggttaaac tctaaaaggg ggaagccaac gaaattatta attcggaata gtgatggtct
    15421 tctgaaagaa gtaagcgtta atctaaaagt ggtgacattt aactttggtg taaatgaatt
    15481 agcgctaaaa atgggcttag gctggaggaa tgttgacaag cttaatgatg aatcaatatg
    15541 ttctttgttg ggggataatt tcctcaaaaa tggtgtgatt ggaggttggg ctgcagaagc
    15601 aatagaaaaa aatccaccat gtaagaatga tgtgatatat ctggctaacc agataaaaga
    15661 gattgtaacc aaaaagttac aaaaaaatga taatggagag ccgtataaat tgtcacaaag
    15721 aatgactcta ttggcttata ctataggtgc tgtaccttgc tggaattgta agagtgggaa
    15781 ggacagaaca ggtatgcagg atgctgaaat taaaagagaa ataataagaa aacatgaaac
    15841 aggtcagttt tctcaattaa atagtaaatt atcctcagaa gagaaaagat tattttctac
    15901 tattctaatg aatagtggta atatggaaat ccaagagatg aatactggtg tgcccggaaa
    15961 taaagtcatg aaaaaattgc cgctatcttc gttagagcta tcttattctg aaagaatagg
    16021 ggacccaaaa atatggaata tggtgaaagg gtattcgtca tttgtataag aggaatatat
    16081 ggaagattta gcagatgtta tttgccgagc cttgggtatc cctttaattg atatcgatga
    16141 tcaagcaatc atgcttgatg atgatgtgct aatatatatt gaaaaagaag gagattcaat
    16201 aaatttatta tgcccttttt gtgcattgcc tgaaaatata aatgatctta tatatgcgtt
    16261 gagcctaaat tactcagaaa agatatgctt agctactgat gatgaaggcg gaaatctaat
    16321 tgcgcgatta gatcttaccg ggataaatga attcgaggat gtatatgtta atacagaata
    16381 ttatatttcg cgtgtgcgtt ggctgaagga tgaatttgcg cgaagaatga aggggtatta
    16441 atgtcccgtt tcgtatttat tttgctgtgt tttatcccat atttagggag agctgattgt
    16501 tgggataagg ctggtgaaag gtacaatatt ccatcaagtt tgttaaaagc gattgcggaa
    16561 aaagaatccg gatttaataa atctgcagtc aatgttaata acaatggaag taaagattat
    16621 ggtataatgc aaataaatga ctttcattct aaaagactta gagaaatggg atattctgag
    16681 gaaatgctaa ttagtcatcc atgtctttct gtacattatg cagcaaagtt attaaatgag
    16741 tttatgatga tgtatggaag agggtgggag gctgttggag cctataatgc aggaacgtct
    16801 ccgaaaaaga aaaaagaaag attaaagtac gcagaggata tctatagacg ctatttgcgg
    16861 atagcagcag agagcaagca gaataatcga aggatataag aggatttatg tctgaggcaa
    16921 agaactcaaa tcttgcacca ttcaggttat tagtgaaact cacaaacggt gtcggggatg
    16981 agtttccgtt atattatggg aataacttaa ttgttctcgg acggacgata gaaacacttg
    17041 agtttggaaa cgacaatttc ccagaaaata ttattcctgt aacagatagt aaatcggatg
    17101 gtataattta tttgaccata agcaaagata atatctgtca atttagtgat gaaaaaggtg
    17161 agcaaattga tatcaattca caatttaatt catttgaata tgacgggatt tcttttcacc
    17221 ttaaaaatat gagggaggat aaatcgagag gccatatact aaacggaatg tataagaacc
    17281 actcggtgtt ttttttcttt gctgttattg ttgtgttaat tataattttt tccctctctt
    17341 taaagaaaga tgaagtaaaa gaaattgctg aaattataga tgataagaga tatggaatag
    17401 tcaacactgg acagtgcaat tatattcttg ccgaaacaca aaatgatgcg gtatgggcgt
    17461 ctgtagcctt aaataaaacc ggatttacca aatgcagata tattctggtg tcaaacaaag
    17521 aaataaatag aattcaacaa tatatcaatc aacgattccc atttatcaat ttgtatgtgt
    17581 tgaatttagt gagtgataaa gcggaattac tggtgttttt aagtaaagaa agaaatagtt
    17641 caaaagatac cgaacttgat aaattgaaaa atgctctgat tgttgaattt ccgtatatca
    17701 aaaatattaa attcaactat cttagcgatc ataatgcaag gggggatgct aaaggtattt
    17761 tcacaaaggt aaatgtccag tataaagaga tttgtgaaaa taataaagtt acatattctg
    17821 tcagagaaga actgacagat gaaaagcttg agctcattaa tagactgatt tctgaacata
    17881 aaaatatata tggagatcag tatattgagt tttctgtact tttgatagat gatgatttta
    17941 aaggtaaatc atatcttaac agcaaagaca gttatgtaat gttgaatgat aaacactggt
    18001 tttttttaga taaaaataag tgaggataaa atgagtgtta cagtaccgaa tgatgattgg
    18061 acattgagtt cattatctga aacttttgat gatggaactc aaacattaca aggtgaacta
    18121 acattggcac tagataaatt agctaaaaat ccttcgaatc cacagttgct ggctgaatac
    18181 caaagtaaat tatctgaata tacattatat aggaacgcgc aatccaatac agtgaaagtg
    18241 attaaggatg ttgatgctgc aattattcaa aacttcagat aatagggagc attcatgaat
    18301 tacatttatc cagtcaatca ggttgatatt atcaaagcca gtgattttca atctcaagag
    18361 atatcaagtc tggaagacgt cgtgtcggct aaatatagtg atattaagat ggatacagat
    18421 attcaagtat cacaaataat ggagatggta agcaatccag aatcattaaa cccagaatct
    18481 ttggccaagt tacagacgac gctctcaaat tattcaatag gagtatcatt agctggcacg
    18541 ttagcaagaa aaacagtttc ggctgttgaa actttattaa agtcttaatt tatatgatta
    18601 ggtataaagg ttttatttta ttcttgttgc tgatgttgat tggatgtgag caacgtgaag
    18661 agttaatttc taatttatct caaagacagg caaatgaaat aatatctgtg ctagaacgcc
    18721 ataatattac tgctagaaaa gttgatggag gtaaacaggg gatctcggta caagtcgaaa
    18781 aggggacatt tgcatcggca gttgatttga tgcgcatgta cgatttgcca aatccggaga
    18841 gagttgatat ctcacaaatg tttcctacag attcattagt gtcttctcca agagctgaaa
    18901 aggcccgttt atatagtgct attgagcaac ggctggaaca gtctttagtt tctattggtg
    18961 gtgttatttc ggcaaaaata catgttagct atgatcttga agaaaaaaat atatcttcaa
    19021 aaccgatgca tatatcagta atcgctatat atgactcacc gaaagagtct gaactattag
    19081 ttagtaatat taagcgattt ttgaaaaaca ccttttctga tgttaagtat gaaaatatat
    19141 ctgtcatatt aactccgaaa gaagaatatg tttatacaaa tgtacaacct gttaaggaag
    19201 ttaaatcgga atttttaaca aatgaagtaa tatatttatt tctcgggatg gctgtactag
    19261 ttgtcattct tttggtatgg gcattcaaaa cagggtggtt caagagaaac aaaatatgat
    19321 aagaatggat ggaatttata aaaaatatct ttcaataatt tttgatccag cgttctatat
    19381 aaatagaaat cggttgaatt tgccttctga actgttagaa aatggcgtaa tcagaagtga
    19441 gattaataat ctcataatta ataaatatga tctaaattgc gatattgaac ctttaagcgg
    19501 ggtaaccgct atgtttgttg ccaactggaa tttacttcca gctgttgctt attttatcgg
    19561 tagtcaagag tctcggttaa ttaatcatag tgagatggtt attagttatt atgggggcaa
    19621 aatcagtaag caaggggagg ccgctattcg aagtggtttt tggcatctta ttgcgtggaa
    19681 ggaaaatata tctgttggta tatatgaaag aataaactta ttatttaatc caattgcttt
    19741 ggagggaaat tatacgcctg tagaaagaaa tttgtcccga ttaaatgaag gtatgcaata
    19801 tgcaaaaagg cactttaccg gtatccagac atcatgccta tgatggtgtt gttataaaga
    19861 gaattgaaaa ggaattatgt aaaaccataa aagatagaga cactgagagt aagaagaaag
    19921 caatttgtgt tataaaggag gctacgaaaa aagctgaatc actgcgtata gatgctgtat
    19981 gtgatggtta tcaaattggt atacagactg cttttgagca tattattgac tatatttgtg
    20041 aatggaagtt aaagcaaaat gaaaatagaa gaaatattga ggactatata actagtttgc
    20101 tttcagaaaa tttacatgat gagaggatca ttagcacttt attggagcag tggcttagtt
    20161 cgttgcgtaa tacagtaaca gaattaaaag tggttttgcc caagtgtaat ttagcactga
    20221 ggaaaaagtt agaacttgat ctccataaat atcgctcaga tgtcaagatt attttaaaat
    20281 attctgaagg gaataattat atattctgta gtggtaatca agtagtggag tttagtcctc
    20341 aggatgtaat atcaggtgtt aaaatagaac ttgcggaaaa attaacaaaa aatgataaaa
    20401 aatatttcaa agagttagca cataagaaac tgagacaaat tgcagaagac ttgttaaaag
    20461 agaatcctgt taatgattaa tcaaataaat gcaagcaatg ctctgcagca aagattaaat
    20521 agtgaagaag ttgttaattt aaatgagaga ttatcatctt cgcaatcatt tgatgaggat
    20581 ataatctatg aaataatgca atatttctca cagagtgaat taaattccat agataatgat
    20641 gaactgcaca ataagattga acaattattc aattcacgtt ttccttatct tacagctgca
    20701 caaaaaagtt cacttcttaa taaactcata gatgcaaatc aatatgtcga tttgcatgag
    20761 ggattttatg catcattgtc catatataac aatattgatt tctatattaa gactacaacc
    20821 ttcgattcat taattagtgt ctttgaagca gggagagagg cagatgattc gacatggtag
    20881 taataagttg aaaatattta ttttaagtat attgctatta acactgagtg ggtgtgcttt
    20941 aaagtcatca tctaattctg aaaaagaatg gcatattgtt cctgtaagta aggattattt
    21001 ttctattcca aatgatttat tatggtcgtt taatacaacc aataaaagta taaatgttta
    21061 ctctaaatgt attagtggta aggcggttta tagttttaat gcaggtaaat tcatgggcaa
    21121 ctttaatgtt aaggaagtag atgggtgctt catggatgca caaaagatag ctatagataa
    21181 actattttct atgctgaaag acggggttgt tttaaaaggt aataagataa atgataccat
    21241 ccttatagag aaggatgggg aagttaaatt aaaattaatt cgagggatat aattgtattg
    21301 tgagtaaata taaaggtcta aatacaagta atatgtttta catttactct agtggacatg
    21361 aaccagttaa cgttgagctt gtaaaagata aagaacgtaa cataattgag ctggctccag
    21421 catggaaggg tttttttttg tccgaaatca aaatataaaa ttcagtgata atgtgaacta
    21481 tcattatcgc tttaacatta atagttgtgc aaaatttctt gctttttggg actatttcag
    21541 tggtgctcta gtagagcata gccatgctga aaaatgtatt catttctatc atgagaatga
    21601 tcttcgggat agttgtaata ccgaatcgat gcttgataag ttaatgttaa gattcatctt
    21661 ttcatctgac cagaatgtaa gtaatgcact ggctatgata cgaatgactg agtcatatca
    21721 tttggttctt tatcttctga gaactattga gaaagaaaag gaggtaagaa taaagagtct
    21781 gacagagcat tatggtgtgt cggaggctta ttttagatct ttatgtagaa aagctttagg
    21841 ggcaaaagtc aaagaacaac tgaacacttg gcggttagta aatggtttat tagatgtatt
    21901 tttgcacaat caaacaatca cttcagctgc catgaataat gggtatgcat caacatccca
    21961 tttttcaaat gagataaaaa cacgactagg cttttctgca agagaattat cgaatattac
    22021 ttttttagtg aaaaaaataa atgaaaaaat ttaatattaa atctttgact ctcttgattg
    22081 tattgttacc attgatagtt aatgctaata atattgactc tcatttatta gaacaaaatg
    22141 acatagcgaa atatgttgct caaagtgata cggtaggatc tttttttgaa cgtttttctg
    22201 cgttattaaa ttatcctatt gttgttagca aacaagctgc aaaaaagaga atatctgggg
    22261 agtttgattt atctaatcca gaagaaatgc ttgaaaaact gacattactt gtgggactca
    22321 tatggtataa agatggaaat gcattataca tatatgactc tggagaatta ataagcaaag
    22381 ttattttgct ggagaatata tcacttaatt atcttattca gtatctgaag gatgctaacc
    22441 tttatgatca cagatatcct ataagaggaa acattagtga taaaacattc tatatatctg
    22501 gtcctccggc attggttgaa ctggtagcta atacagcaac tttgcttgat aagcaggttt
    22561 cttctattgg gacagataaa gttaattttg gggttataaa attaaaaaat acatttgtta
    22621 gtgacagaac ttataatatg agaggggagg atattgtcat cccaggggtg gctactgttg
    22681 tagaacgttt attaaataat ggtaaggctc tgagtaacag acaagcccaa aatgatccga
    22741 tgccaccctt taatattaca cagaaagtat ctgaggatag taatgacttt tcatttagtt
    22801 cagttaccaa tagttctata ttagaggatg tatcacttat tgcatatcca gaaaccaatt
    22861 ctatattagt caaaggtaat gatcagcaaa tacagattat tcgcgatatt atcactcagt
    22921 tagatatagc caaacgacat atagaattat cattatggat aatcgatatt gataaatctg
    22981 aattaaataa ccttggtgtt aattggcaag gaacggcttc atttggtgat tcgtttggtg
    23041 cgtcatttaa tatgagctca tcagcatcga taagcacttt ggacggcaat aagtttattg
    23101 cttcagttat ggcattaaat cagaaaaaga aagcaaatgt agtttcaagg ccggtgattt
    23161 tgacacaaga gaatattccg gctatatttg ataataacag gactttttat gtgtcattag
    23221 ttggtgagcg caattcttcg ttggagcatg ttacgtatgg aacattgata aatgttatac
    23281 ctcgcttttc atctcgggga caaattgaga tgtctttaac gatagaggat ggaacaggaa
    23341 atagtcagtc aaattacaat tataataatg aaaatacatc tgtccttcca gaagttggaa
    23401 ggaccaaaat cagtacaata gcgagagttc cacaaggcaa aagtttgttg ataggtggtt
    23461 atacgcatga aacgaactct aacgaaataa tatctattcc ttttctctcc tctattcctg
    23521 taatcggtaa tgtgttcaag tataaaacaa gtaatatcag taatattgta agggtttttc
    23581 tgattcaacc ccgtgagata aaggagtcaa gttattataa tactgcagaa tataaaagcc
    23641 tcatatctga aagagaaata caaaagacta cacaaataat cccatctgaa actactttgc
    23701 tggaagacga aaaatcattg gtttcatact taaattacta actataaagt aggtgatgta
    23761 tgcttgatgt taaaaataca ggagttttta gctctgcatt cattgataag ttgaatgcaa
    23821 tgacaaattc agatgatgga gatgagactg ctgatgcaga gcttgattct ggcttggcta
    23881 atagcaagta tattgactca tctgatgaga tggcttccgc tctttcgtca tttataaaca
    23941 gaagagacct tgagaaactg aaaggaacaa atagtgatag tcaggaacgt attttagatg
    24001 gggaagaaga tgaaattaat cacaagattt ttgatttaaa gagaacgtta aaagataacc
    24061 ttcctctaga tcgggatttt atagacagac taaagagata ttttaaagat ccaagtgatc
    24121 aagtcttagc attaagggaa cttttgaatg aaaaagatct tactgctgaa caagtcgaat
    24181 tattaactaa aattattaat gagataatat caggtagtga aaaaagtgtt aatgctggaa
    24241 taaattcagc tatacaggct aaattatttg gcaacaaaat gaaacttgaa ccacagcttt
    24301 tgcgtgcatg ttatcgtggt tttatcatgg ggaacatatc aacaacagat cagtatatag
    24361 aatggcttgg taattttggt tttaatcaca gacatacaat tgtgaatttt gtagagcagt
    24421 cactgattgt agacatggat tctgagaaac cgagctgtaa tgcttatgag tttggttttg
    24481 tgttatctaa attaattgca attaagatga ttagaacttc agacgtaatt tttatgaaga
    24541 aactggaatc ctcaagcttg ctaaaagatg gcagtttaag tgcagagcag ctattgctaa
    24601 ctttattata tatttttcaa tatccaagtg aaagtgagca aattcttact tctgttatag
    24661 aagtatcacg agccagtcat gaggattctg tagtgtatca aacatatcta tcttctgtta
    24721 atgaaagtcc tcatgatata tttaaaagtg aaagtgaaag agaaattgcg atcaatattc
    24781 tacgagagct tgtcacaagt gcatacaaga aagagctttc tagataacag gagataaaag
    24841 tgatccagtc ttttcttaag caagtaagta ctaagcctga attaattata ttagtgctta
    24901 tggttatgat cattgctatg ttgatcatac cattacctac ataccttgtt gattttttaa
    24961 tagggcttaa tattgtactt gctattctcg tttttatggg atcattttat attgaaagaa
    25021 tattgagttt ttctacattc ccttcggttt tgttgataac gacattattt cgtctcgctt
    25081 tatcaataag tactagtcga ctaattttag ttgatgcgga tgcgggtaaa attattacta
    25141 catttggcca gtttgtcatt ggtgatagtc tggctgtagg ttttgttatt ttttcaatag
    25201 taactgttgt gcagttcatt gttataacaa aaggatctga aagggttgcg gaggtagctg
    25261 ctcgcttttc tcttgacggt atgccgggga aacaaatgag cattgatgcg gatttaaagg
    25321 ctggaattat tgatgccgca ggagctaaag aaagacgcag tattctggaa cgtgagagtc
    25381 aattgtatgg ttcatttgat ggagcaatga agttcattaa aggtgatgca attgctggca
    25441 tcattattat ttttgtcaat ttaataggag gtatttctgt tggtatgagt caacatggaa
    25501 tgtccctatc tggtgcatta tctacttata ccatactaac tattggtgac ggattggtct
    25561 ctcagattcc tgctctgtta atttctataa gtgcaggatt tatcgttaca agagttaatg
    25621 gtgatagcga taatatggga cgtaacataa tgtcccagat atttgggaat ccttttgttc
    25681 tcattgttac atcagctctt gccttggcga tagggatgtt gccaggtttt ccgttttttg
    25741 tttttttcct gatagcagtt actttgacgg ctttattcta ttataaaaag gtcgtagaaa
    25801 aagaaaaaag tctgtctgag tctgattcta gtggctatac tggtacattt gatattgata
    25861 atagccatga ctcatctttg gcaatgatag aaaatctgga tgctattagt tcagaaaccg
    25921 ttcctttaat tttattattt gccgaaaata agataaatgc aaatgatatg gaaggtctta
    25981 ttgaaaggat aagaagtcag ttctttattg attatggtgt caggcttcca actattttat
    26041 atagaacaag caatgagctt aaggtcgatg atattgtttt gttaataaat gaggtgcgtg
    26101 ccgatagttt taatatatat tttgacaaag tgtgtattac agatgaaaat ggagatatag
    26161 atgctctagg tattcctgtt gtttcaactt catataatga acgtgttatt tcttgggtag
    26221 acgtttcata tacagaaaat ctaactaata ttgatgctaa aattaaaagt gctcaagatg
    26281 aattttatca ccagttgtca caagctttat taaacaacat aaatgagatt tttggtatac
    26341 aagaaacaaa aaatatgtta gatcagtttg aaaatcggta tcctgatcta ttaaaggaag
    26401 tcttccgaca tgtgactata cagagaattt ctgaggtatt acaaagattg cttggagaaa
    26461 atatttctgt tcgcaattta aaacttatta tggagtcttt ggcgctttgg gctcccagag
    26521 aaaaagatgt cataacatta gttgaacatg tccgtgcatc actttctagg tatatttgta
    26581 gtaaaatagc tgtttctggt gagattaaag ttgtgatgct ttccggatat attgaggatg
    26641 caataagaaa agggataagg caaacctctg gtggctcttt cttgaatatg gatatagagg
    26701 tttcggatga ggtaatggaa actttagcac atgctttgag agaattgaga aatgcaaaaa
    26761 aaaatttcgt tcttttggta tcagtagata tacgtaggtt tgttaaaaga cttatagata
    26821 acagatttaa gagtatactc gttatatcgt atgctgagat tgatgaagca tataccatta
    26881 atgtattaaa gactatttag tgaggtttaa atatgagtaa cattaattta gttcaattag
    26941 ttagagatag tcttttcacg attggttgtc ctccatcaat tattacagat ttagacagcc
    27001 attcagcgat taccatttct ttggatagta tgcctgctat taatatagct cttgtcaatg
    27061 agcaggttat gttatgggct aattttgatg caccaagtga tgtaaaactt cagtcatctg
    27121 cttataacat attaaattta atgttaatga actttagtta tagcattaat gagctggtag
    27181 agcttcatcg ttctgatgag tacttacagt taagagttgt tattaaagat gactacgttc
    27241 acgatgggat tgtttttgca gagattctgc atgagtttta tcagaggatg gaaatcttaa
    27301 atggggtctt ataatcaatg agctatacaa aattgctcac tcaattatct tttcctaata
    27361 gaatctcggg gccaatcttg gaaacaagtc ttagcgatgt ttcgattggt gagatttgta
    27421 acattcaggc tggaattgaa agtaatgaaa ttgttgcaag agctcaggtt gtaggatttc
    27481 atgatgaaaa aacaatatta agcttgattg gaaattctcg tggactttca cggcaaacgt
    27541 tgattaagcc cactgcccag tttcttcata cgcaagtcgg ccgtggatta ttgggagcag
    27601 tagtcaatcc tttaggggag gttactgata agtttgctgt tacagataac agtgaaattc
    27661 tttatcgacc tgtagataat gctcctccgc tatatagtga aagggctgca attgagaagc
    27721 cttttttaac aggtattaag gttattgatt ctttactcac gtgtggtgaa ggacagcgaa
    27781 tggggatttt tgcgtcagct ggttgtggca aaacttttct catgaatatg ctcattgaac
    27841 atagtggtgc tgatatatat gttattgggt taattggtga gcgaggtcga gaggttactg
    27901 aaacggttga ttatttgaaa aactctgaga aaaaaagcag gtgtgtttta gtatatgcaa
    27961 cttcggatta ctcttcggtt gatcgttgta atgctgcata tatagccact gctatagccg
    28021 aattttttag gactgaagga cataaagtag cgctttttat tgattcatta acaaggtatg
    28081 ccagagcatt acgtgatgtg gccttagccg ctggagaatc acctgccaga agaggctatc
    28141 cggtttcggt ttttgatagc ttacccagac ttcttgaaag gccaggaaag ttaaaggcag
    28201 gtggctctat tactgcattt tacactgttc ttttggagga tgatgatttt gctgatccat
    28261 tagctgaaga ggtaagatcc attttagatg gacatatata tttgagcaga aatctagccc
    28321 aaaaaggaca atttcctgca attgattcct taaaaagtat aagcagggta tttacacagg
    28381 ttgttgatga aaaacatcgt attatggccg ctgcatttcg ggagctactt tctgaaatag
    28441 aagagctaag gacaattatt gactttggtg aatacaaacc gggggagaat gcctctcagg
    28501 ataaaatcta caacaaaata tctgttgttg agagttttct gaagcaagat tatcgactcg
    28561 gttttactta tgagcagaca atggagctta ttggtgaaac aattagataa ggttttaaaa
    28621 atcaaagata aatatcaacg gtctgttaaa ttaattgaag cacacatatt aacactaaaa
    28681 aaaaaactct ttgtatcgag atgtggaggc attagataaa aggattatat acttcctgca
    28741 actggagaat gacttagaac ctgtaggcgc tcagagtgtt agtcagcttt tcaatacaag
    28801 acgtaagatc gctatagtaa aaaagcatat tatccaatat cagtctgaac gaattcttct
    28861 aaagggaaga attgaagaaa ttcaaaaaga tattgatgag gcaaatgctt ctaaaagaaa
    28921 actgcttcat aaagagagta aaatatgtaa acggattggc ttgattaaaa gaaataactt
    28981 tgccaaacag ttgatacttg atgagttatc acaagaggat atgaagtatg gcattagata
    29041 atataaacct aaattttagt agtgacaaac agatagaaaa atgtgagaaa ctatcttcaa
    29101 tagataatat tgatagtctc gttttgaaga agaagaggaa ggttgaaatt cctgagtact
    29161 ctttaattgc atctaactat tttactattg ataagcactt tgaacataag catgataaag
    29221 gagaaattta tagtggcatt aaaaatgcgt tcgaacttag aaacgaacga gcgacatatt
    29281 ctgatattcc agaatcaatg gccattaaag aaaatatttt gataccagat caagatatca
    29341 aagcaaggga aaaaataaat atcggcgata tgagggggat cttttcatat aataagagtg
    29401 gaaatgcaga caagaacttc gaaagaagtc atacttcttc tgtaaaccct gataatctgc
    29461 tagaatctga taatagaaat ggtcaaattg gtttaaaaaa tcatagcttg tctattgata
    29521 agaatattgc tgacatcatt tctttactaa atggaagtgt tgctaaatca tttgagctgc
    29581 ctgtaatgaa taaaaatact gcagacataa ccccatccat gtcattgcaa gaaaaatcaa
    29641 tagttgaaaa tgataaaaat gtttttcaaa aaaatagtga aatgacttac cactttaaac
    29701 agtggggggc tggacattct gttagtattt cagtggagtc tggttctttt gttctaaaac
    29761 cgtcagatca atttgtagga aataaacttg acttaatttt gaaacaagat gctgagggta
    29821 attacagatt tgatagcagt caacataata aggggaataa aaataatagt acaggatata
    29881 atgaacagag tgaagaagaa tgctaagaat taaacatttt gacgctaacg aaaaactaca
    29941 gattttatat gcaaagcaac tctgcgagcg tttttcgatt cagacattca aaaataaatt
    30001 tacaggcagt gaaagtttag tcactcttac ttccgtgtgt ggggattggg taattcgtat
    30061 tgatacatta tcttttttga aaaaaaaata cgaggtattt tcaggatttt ctacacaaga
    30121 atctttactg catttatcaa aatgtgtctt tatagagtcg tcatctgtat tttcgattcc
    30181 agaactgtct gataagatta ctttccggat cacgaatgaa atccagtatg caactactgg
    30241 gagtcattta tgctgttttt cctcttcttt aggtattatt tattttgaca agatgccggt
    30301 attacgtaat caagtttctc ttgactcatt gcatcatctt ttagagtttt gcttaggttc
    30361 atctaatgta aggctggcta ctttaaaaag aattcgcact ggtgatataa tcatagttca
    30421 gaaactttat aatttattat tgtgtaatca agttattatt ggggattata ttgtgaatga
    30481 taataatgag gcaaaaatta atctgtcaga aagtaatggt gagtcagaac acacagaagt
    30541 ttctttggca ttattcaatt atgatgatat caatgtaaaa gtggacttta ttcttttaga
    30601 aaaaaatatg acaatcaatg aactaaaaat gtatgtagaa aacgaattat tcaagtttcc
    30661 cgatgacata gttaaacatg taaatattaa agtaaatggt tctttggttg ggcatgggga
    30721 acttgtttct attgaggatg gttatggtat cgagattagt tcttggatgg taaaggagta
    30781 atctcatgct gagtgacatg tccctcatcg ctacactttc tttttttacg ctattaccat
    30841 ttttagttgc tgctggtacc tgttatataa agttttctat tgtttttgta atggtacgaa
    30901 atgcacttgg tttgcagcag gttccatcaa atatgactct caatggtatt gcattgatta
    30961 tggctttgtt cgtgatgaaa cccattattg aagctggtta tgaaaattat ctaaatggcc
    31021 ctcaaaaatt tgatacaata tctgatattg tccgtttttc tgattctggc ttgatggaat
    31081 ataagcaata cttgaaaaag catacagatc ttgagttagc acgttttttc caaaggtctg
    31141 aagaagaaaa tgctgattta aagagtgcag agaataatga ttattcattg ttttccctct
    31201 tgcctgcata tgcgctaagt gaaatcaaag atgcgttcaa aataggtttt tatttatatt
    31261 tgccatttgt tgttgttgat cttgttattt caagcatttt acttgcactt ggtatgatga
    31321 tgatgagtcc aatcacaata tcagtaccaa taaaacttgt attatttgtt gcgcttgatg
    31381 ggtggggtat cctgtctaag gccttgattg aacaatatat caatattcct gcttagatga
    31441 tggaggtaat gtgtctgata tagtttatat gggtaataag gctctttatt taatccttat
    31501 cttttcctta tggccagtag gtatagctac ggttattgga ttatctattg gtttgttaca
    31561 gacagtgact caactccaag agcagacact tccttttggt ataaagctta taggtgtctc
    31621 aatatctttg ctacttcttt ctggatggta tggtgaggtt ttattgtctt tttgtcatga
    31681 aataatgttt ttaattaaga gtggggtttg atggacattt caagctggtt cgaaagtatt
    31741 catgtgtttt taatactcct gaacggcgtt ttttttagat tggctccatt atttttcttt
    31801 cttccatttt taaataacgg tataatttct ccatctatta gaatacctgt gatttttctt
    31861 gttgcatcag gattaattac ttctggtaag gtagacatag gttcttctgt ttttgaacat
    31921 gtttatttcc ttatgttcaa ggaaataatt gttggcctcc ttctctcttt ttgcttgtct
    31981 cttccctttt ggatatttca tgctgttggt agcattattg acaaccagcg tggggcaacg
    32041 cttagtagtt caattgatcc tgccaatggt gttgatacgt ctgagttggc aaaatttttc
    32101 aatctttttt ctgcagttgt atttctatac agtggtggta tggtctttat tttagaatcc
    32161 atacaattgt cttataatat atgcccgtta ttttctcaat gttctttccg tgtctcaaat
    32221 atcttaacat ttctgacttt attggcaagt caggctgtta ttttagccag tcctgttatg
    32281 atagtattgt tactatcaga agtattactt ggtgtattat cgagatttgc tccgcagatg
    32341 aatgcttttt ccgtatcatt aactattaaa agtttacttg caatatttat tattttcatc
    32401 tgttcttcta ctatttactt ttctaaagtt caatttttcc tcggtgaaca taaatttttc
    32461 acaaatctat ttgttagata aaatattatg gcaaataaaa cagaaaagcc gacacctaaa
    32521 aaactaaagg atgccgcaaa aaaaggacag tcatttaaat ttaaggattt aacgactgtt
    32581 gttattattc tggtagggac atttactata atatcattct tttccttaag tgatgtaatg
    32641 ctcttataca gatatgtaat aattaatgac ttcgaaatta atgagggtaa atactttttt
    32701 gcagtggtta ttgtcttttt taagataatt ggcttcccac tttttttctg tgttctttcg
    32761 gctgtgttgc caacattggt tcaaacaaag tttgttcttg cgactaaagc tatcaagatt
    32821 gatttttcag tattaaaccc tgttaaaggg ttaaaaaaaa tatttagtat aaagacaata
    32881 aaagaatttt tcaaaagcat tctgcttctt attattctag cattaacaac ctatttcttt
    32941 tggattaatg accgaaaaat aattttttct caggtgtttt ctagtgttga tggcttatat
    33001 cttatttggg ggaggctgtt taaggatata atattatttt tcttggcatt ttctattctt
    33061 gttattattc ttgactttgt gattgagttc attttataca tgaaagatat gatgatggat
    33121 aaacaggaga taaaaagaga atatatagag caagagggac actttgagac aaagtcgaga
    33181 aggcgtgagt tgcatatcga gattctttca gagcagacta aatctgatat acgtaattca
    33241 aaattagtgg taatgaaccc gactcatatt gcaattggta tttattttaa tccagaaata
    33301 gcgcctgcac cttttatttc tctcattgaa actaaccagt gtgccttggc tgtcagaaaa
    33361 tatgcaaatg aagttggtat accgactgtg cgtgatgtga aattagctag aaaactatat
    33421 aaaacacata caaaatatag ttttgttgat tttgaacact tggatgaagt cctacgtctt
    33481 attgtttggc ttgagcaggt tgaaaacact cattaataag taaaggagat gtgttatatg
    33541 ggagttaatt tctgtaataa aataggtatt gatcagtctg aatttgaaat agaatcttcc
    33601 atcattaact ccattgctaa tgaggtattg aacccaatat ctttccttag caataaggat
    33661 ataataaatg ttttactcag aaagatttca tctgaatgtg acctggttag aaaagacatt
    33721 tatcgttgtg ctctggagtt ggtcgttgaa aaaacacctg atgatttata atcattcata
    33781 ataactcttt tgctgatata aatatttttg ttgaagatat ttatgtagtc acaggatcaa
    33841 ttgtagaaaa ttgttatgtt tttgttagga actgcactaa ataatatatg ataagacagc
    33901 aaaaacgact gaccataatc cttcttttat taggagttga taagagagac tattcatcct
    33961 gtaatgttaa aactctgtta tatagtattc gcgattatgc aaagtctgta aatgatcatg
    34021 agatactaac cgagagtaat cgtttattat ctcattgcat ttcagatagt aacggagctt
    34081 tctttaaaag tagtaaatac gttcccctta agtacttacg taaaaggaga atagcacgaa
    34141 agataccaaa tgattagcac tttgctagta agcttgctgg taaactatat tgaccgttgg
    34201 gtttgttttg agactgtaat ttaaattgtg taattgcctg tttttgatat gtttactcca
    34261 acaacggaga caggtaaatt atggacgaaa agaagcttaa agccct
//
LOCUS       DAJTSF010000038        33534 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000038 DAJTSF010000000
VERSION     DAJTSF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.038"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="OO335_001896"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="OO335_001896"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6002306.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="OO335_001897"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="OO335_001897"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6002307.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO335_001898"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO335_001898"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6002308.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO335_001899"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO335_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6002309.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO335_001900"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO335_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6002310.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO335_001901"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO335_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR6002311.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="OO335_001902"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="OO335_001902"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6002312.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="OO335_001903"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="OO335_001903"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6002313.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="OO335_001904"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="OO335_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6002314.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO335_001905"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO335_001905"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6002315.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO335_001906"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO335_001906"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6002316.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="OO335_001907"
     CDS             8212..9075
                     /locus_tag="OO335_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6002317.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="OO335_001908"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="OO335_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6002318.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="OO335_001909"
     CDS             10411..11028
                     /locus_tag="OO335_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6002319.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO335_001910"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO335_001910"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6002320.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="OO335_001911"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="OO335_001911"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6002321.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO335_001912"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO335_001912"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6002322.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="OO335_001913"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="OO335_001913"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6002323.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="OO335_001914"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="OO335_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6002324.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="OO335_001915"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="OO335_001915"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6002325.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO335_001916"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO335_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6002326.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="OO335_001917"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="OO335_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6002327.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="OO335_001918"
     CDS             21859..23568
                     /locus_tag="OO335_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6002328.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO335_001919"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO335_001919"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6002329.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="OO335_001920"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="OO335_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="OO335_001921"
     tRNA            27625..27719
                     /locus_tag="OO335_001921"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="OO335_001922"
     CDS             27991..29202
                     /locus_tag="OO335_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6002330.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29614..30657
                     /locus_tag="OO335_001923"
     CDS             29614..30657
                     /locus_tag="OO335_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6002331.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31172..31600
                     /locus_tag="OO335_001924"
                     /pseudo
     CDS             31172..31600
                     /locus_tag="OO335_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31630..32794)
                     /locus_tag="OO335_001925"
                     /pseudo
     CDS             complement(31630..32794)
                     /locus_tag="OO335_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32859..33507
                     /locus_tag="OO335_001926"
                     /pseudo
     CDS             32859..33507
                     /locus_tag="OO335_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8403 a         8812 c         8264 g         8055 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcaa actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa
    29401 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
    29461 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
    29521 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
    29581 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
    29641 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
    29701 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
    29761 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
    29821 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
    29881 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
    29941 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
    30001 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
    30061 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
    30121 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
    30181 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
    30241 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
    30301 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
    30361 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
    30421 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
    30481 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
    30541 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
    30601 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
    30661 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
    30721 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
    30781 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
    30841 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
    30901 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
    30961 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
    31021 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
    31081 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
    31141 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
    31201 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
    31261 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
    31321 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
    31381 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
    31441 tgttacaccg atagtgacgc cagccttgct aagccgtgag ggttttttat atccgacgcc
    31501 agcgacctcc attcccagag acctgcttga acctttgcca aaaggagcat tttcgattgg
    31561 tgagattggg cagtatgaca aatacaagac gacccatact gatcctaccc acgtaatgtg
    31621 gacacggccc taagcgagat tatggttttc aaattgttcc gggctgagac cgccacaggc
    31681 actgtgacga cgccagcgat tgtaatcaca ctcgatataa ttaaacaccg ttgcccgcat
    31741 tatttcccgg ctgctaaagc gttccccgtg gatacattcc accttcagcg aatgaaagaa
    31801 gctttccaca caggcattgt cataacagta gcctttcgca ctcatactgc cacgcaggtt
    31861 gtgtcgcttc agcattgcct gataatcccc tgaacagtat tgaccaccac ggtccgtatg
    31921 aacaatgacg ctttccgggc gtcttctccg ccacaacgcc atttgcagtg catcacaggc
    31981 cagttgtgct gtcattcgcg gtgacatcga ccagccaatg acggcgcgtg accacaggtc
    32041 gatgactact gcgagataca accagccctc atcggtacgc aagtacgtga tgtcacccgc
    32101 ccacttctgg tttcgggcca ctggcgctga agtcctgctc cagcagattc tccaatacgg
    32161 gcaggccatg tgcacggtag ctgaccgggc tgaacttccg gctggctttc gcccgcagcc
    32221 cctgacgacg caggctggcg gcaatggttt taatattgaa ctccggcagt tcgtcagcaa
    32281 ggcggggagc accgtatcgc tgctttgcct caaagaatgc cttatggaca gcggcatcgc
    32341 aggtgagccg aaactgttgg cgcaggctca tctggtgacg acgcctgagc cagacatacc
    32401 agccgctgcg ggcaacccga agtacacgac acatcgcttt gatgctgaac tctgcccgat
    32461 gattttcgat aaagacatac ttcatttcag gcgcttcgcg aagtatgtcg cggccttttg
    32521 gaggatggac agttcctcag cctgctccgc cagttgtcgt ttaaggcgga cattttcagc
    32581 ggccagttcg ctttcgcgct ctgacgaact catttgttgc tgctgtttac tgcgccaggc
    32641 ataaagctga gattcataca ggctgagttc acgggctgag ttcacgggct gcggcggcca
    32701 caccgatgcg ttcagcgagt ttcagggctt cgttacgaaa ttcaggcgta tgttgtttac
    32761 ggggcttctt gctgattgat actggttttg tcatgagtca cctctggttg agagtttact
    32821 cacttagtcc tgtgtccact attggtgggt aagatcaccc tccattaaaa ctaaaacggg
    32881 cgctttctga tatatcatct gtaagcgcag taataccatt agtagcacct cgcgcattga
    32941 ttattgaata ttataactgg aatgttatat ttcttgttat gatgctgttc agtattataa
    33001 taatgttatt atcaccaaag ataactcatg caaatgagaa agtaatatgt caaaacagtg
    33061 catcctttgt gaccaaagag tttatctgcg gcactatact tgtctcatta atactcagtt
    33121 ctctattttg ctacatatca atatcaccat caattttcat gcatacttat ggcctgagta
    33181 cttttagtta ctcaattatt ttctccttct cagttttatt tttcataatc ggaaatcaat
    33241 tatcaaagat tgaaaaatta tccaacctat ggtttttatt acccaatagc caattttatc
    33301 gcattacaaa taaatggttc aaaaacagga tttgccgcat ctattacagg ttttactcaa
    33361 acaataagcg ctggtacaat cagtttcctg tcagtaaccg taagcgacaa cggtgttggt
    33421 cccggaacta ctttaggtct tggttcacta ataacttcat tactgtcaat agccgtaatc
    33481 gccattgcag gaaataataa aaagtaagtg tactctgact gcgtatgtat ctac
//
LOCUS       DAJTSF010000039        31185 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000039 DAJTSF010000000
VERSION     DAJTSF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.039"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            389..2047
                     /locus_tag="OO335_001927"
     CDS             389..2047
                     /locus_tag="OO335_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6002332.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="OO335_001928"
     CDS             complement(2044..3000)
                     /locus_tag="OO335_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR6002333.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="OO335_001929"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="OO335_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6002334.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="OO335_001930"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="OO335_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6002335.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="OO335_001931"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="OO335_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6002336.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="OO335_001932"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="OO335_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6002337.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="OO335_001933"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="OO335_001933"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6002338.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="OO335_001934"
     tRNA            complement(9859..9946)
                     /locus_tag="OO335_001934"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="OO335_001935"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="OO335_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6002339.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="OO335_001936"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="OO335_001936"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6002340.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="OO335_001937"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="OO335_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6002341.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="OO335_001938"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="OO335_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6002342.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="OO335_001939"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="OO335_001939"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6002343.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="OO335_001940"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="OO335_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6002344.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="OO335_001941"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="OO335_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6002345.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="OO335_001942"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="OO335_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6002346.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="OO335_001943"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="OO335_001943"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6002347.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="OO335_001944"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="OO335_001944"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6002348.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27412
                     /gene="dmsA"
                     /locus_tag="OO335_001945"
                     /pseudo
     CDS             24969..27412
                     /gene="dmsA"
                     /locus_tag="OO335_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27423..28040
                     /gene="dmsB"
                     /locus_tag="OO335_001946"
     CDS             27423..28040
                     /gene="dmsB"
                     /locus_tag="OO335_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6002349.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28042..28833
                     /locus_tag="OO335_001947"
     CDS             28042..28833
                     /locus_tag="OO335_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6002350.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28869..29495)
                     /locus_tag="OO335_001948"
     CDS             complement(28869..29495)
                     /locus_tag="OO335_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6002351.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29810..30958
                     /locus_tag="OO335_001949"
     CDS             29810..30958
                     /locus_tag="OO335_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6002352.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7689 a         7645 c         8373 g         7478 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgt cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagtgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccactgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttatgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctacctat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacga caataccggc gatgacaatt acgacggcct
    25261 acactaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt caccgtgtct acaatccgga
    25321 ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat
    25381 tagctgggaa gaagcctacg acatcatcgc gaccaatatg cagcgcctga tcaaagagta
    25441 cggcaacgag tctatctatc tgaactatgg caccggtacg ctgggcggca ccatgacccg
    25501 ctcctggccg ccgggaaata ccctggtcgc gcgcctgatg aactgctgcg gcggttatct
    25561 taaccattac ggtgactact cttccgcgca aattgcggaa ggtttgaacc atacctacgg
    25621 cggctgggca gatggtaaca gcccgtcgga tatcgaaaac agtaaattgg tagtgctgtt
    25681 tggtaataac ccaggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca
    25741 ggcacgccag aaatctaatg ctcgcatgat catcatcgat ccgcgctata ccgacaccgg
    25801 tgccgggcgc gaagatgagt ggatccctat tcgtccagga acagatgccg cactggttaa
    25861 cggtctggcg tacgtcatga tcactaaaaa cctggtggat caggcattcc tcgataaata
    25921 ttgcgttggc tatgacgaga aaaccctgcc cgccagtgcg ccgaaaaatg gtcattataa
    25981 agcttatatt ctgggtgaag ggccagatgg cgtggctaaa acaccggaat gggcctcgca
    26041 aatcaccggt gttccggcag acaaaatcat caaactggct cgtgaaatcg gcagtaccaa
    26101 accggcgttt atcagccagg gatggggccc gcagcgtcac gctaacggtg aaatcgcaac
    26161 ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt ggtattaatg gagggaacag
    26221 cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt atgccgacct tggaaaaccc
    26281 gatccagacc agcatttcga tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat
    26341 gacggcgctg cgtgatggtg tgcgcgggaa agataagctg gatgtgccga tcaaaatgat
    26401 ctggaactat gccggtaact gcctgattaa ccagcattct gaaatcaacc gtacccatga
    26461 aatcctccag gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc
    26521 atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc gcttccgaac agatggactt
    26581 tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa
    26641 accgcgcttt gaatgtaaga ccatctatga aatgaccagc gaactggcaa aacgtcttgg
    26701 cgttgagcaa cagtttactg aaggccgtac ccaggaagag tggatgcggc atctgtatgc
    26761 ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa gagttccgca agcaggggat
    26821 ctttaaaaag cgcgacccac aagggcatca cgttgcttat aaagccttcc gtgaagatcc
    26881 gcaggcaaac ccactgacta cgccatcggg caaaattgag atttattcgc aggcgctggc
    26941 tgacattgcc gctacctggg aattgccaga aggcgatgtg atcgatccac tgccgatcta
    27001 cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac
    27061 aggtttccac tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc
    27121 ggcttgccgt caggaaatgt ggatcaaccc gcttgatgcc caaaaacgcg gtatccacaa
    27181 cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt catattgagg cgaaagtgac
    27241 gccacgaatg atgccgggcg tggtcgcact gggtgaaggc gcctggtatg acccggatgc
    27301 aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc actcaacgtc cgtctcctct
    27361 cgctaagggg aatccgtcac atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac
    27421 cgatgacaac ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct
    27481 gcgagctggc ttgtaaagac tacaaagatt tgacgccaga agtcagtttc cgccgcattt
    27541 atgaatatgc tggcggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg
    27601 cctactatct gtcgatttcg tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc
    27661 cgagcggtgc gatgcataaa cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca
    27721 ttggctgccg ttactgtcat atggcttgcc cgtatggcgc accgcaatat aacgaaacga
    27781 aaggccatat gaccaaatgc gatggttgtt atgaccgtgt tgcggagggt aaaaagccga
    27841 tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta
    27901 aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga
    27961 atattgtgat caaacccaat gccaatagcc gcccgaccgg ggataccact ggctatctgg
    28021 caaacccgaa ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc
    28081 acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc tggctttggc gctgctcaaa
    28141 ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct gcatgtttgg cttatgggta
    28201 ctgatgggca ttggctttat cgcctctatg ctccatcttg gttcaccgat gcgcgccttt
    28261 aactcgctca accgggtagg ggcttcagca ctcagtaacg aaatcgccag cggttcgatc
    28321 ttttttgctg taggcggcat cggctggttg ctggcaatgc tgaaaaagct gtcaccggca
    28381 ttgcgtacgc tatggctggt ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg
    28441 gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt acagcatctg gacgccgatg
    28501 ggctgggcga tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggcgtg
    28561 atgtcagtga tgcagggcac agagctggca actattcata gttctgtgca gcaggccgca
    28621 gcgctggtgc cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc
    28681 ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc
    28741 gtctcattca ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg
    28801 catatgaccg tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggccccg
    28861 gttctctttt atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca
    28921 ggaatatggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc
    28981 agctcgcaag ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga catgcgatcc
    29041 cacgccgaat gccgggtaat ttcattaaag gtccctgatg catcagtcac gacaaagaca
    29101 tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca
    29161 ccggcaataa ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta
    29221 tcccaggcgt taatattgcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc
    29281 agttctagaa ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag aatagttggt
    29341 aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt
    29401 tcgatatccc gtacaaggga aagtaaacca gcctggtgat caacaagcaa aacggcagca
    29461 tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg
    29521 gtacgtctga aatcggtcag acgcgatgca ttgctctgaa agcatagacg ggaaatatga
    29581 gtttgctgtg accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca
    29641 ataaaaacaa tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata
    29701 taaatcgata ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg
    29761 gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta
    29821 tacccggcct gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt
    29881 gttaaataca ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg
    29941 cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt
    30001 cattaaacgc attggcttta accgcagcta ttatctgtcc tccttcattt ttgctgctgg
    30061 ctgtgccggc cttggcctga tgattggttt ctggagctgg ttggcgtggc gttttgtcgc
    30121 gggcgtcggc tgtgccatga tttgggtggt tgttgagagc gcgctgatgt gcagtgggac
    30181 gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg tgggaacgtt
    30241 tttaggccag ttactggtca gcaaagtctc aaccgagctg atgagcgtat tgccgtgggt
    30301 tacaggtttg acattggcag ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca
    30361 ggctgaaaac catgattcga cgtcaattac ggcaatgcta aaactccgtc aggcgcggct
    30421 tggcgtgaat ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc
    30481 tctgtacctc aatcacaaag gggtgagcaa tgccagtatt ggtttctgga tggcggtact
    30541 ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt ctggcggata agtttggtcg
    30601 actgctggtt ttgcgtgttc aggtctttgt cgtcattctc ggcagtattg cgatgcttag
    30661 ccaggcggcg atggccccag cgttattcat cctcggtgcc gctggcttta cgctatatcc
    30721 ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca
    30781 agccttactg ttgagctata ccgtgggaag tctgcttggc ccgtcattta gcgctatgct
    30841 aatgcagaat ttctccgata atttattgtt tatcatgatc gccagcgtat cgtttatcta
    30901 tttgctgatg ctgcttcgca acgccggtca tacgccgaaa cccgttgctc acgtgtaaat
    30961 gaattcaagc aaagtgtgaa cttactgttt cacactctgc ttttttgttt cttctatctg
    31021 acttgctttt ttccaaattt tattcgttta aaaataaaat gtgcagcagg ttataatttt
    31081 gcatttcgct atttccgcac ttcttatttg ccgcgcataa tccctcgttt tacagatgcc
    31141 cctttaattt tggcgaagga tttgtctatg gctgggaatg ttcag
//
LOCUS       DAJTSF010000040        30096 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000040 DAJTSF010000000
VERSION     DAJTSF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.040"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO335_001950"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO335_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6002353.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO335_001951"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO335_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6002354.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO335_001952"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO335_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6002355.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO335_001953"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO335_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6002356.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO335_001954"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO335_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6002357.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO335_001955"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO335_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6002358.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO335_001956"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO335_001956"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6002359.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO335_001957"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO335_001957"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6002360.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO335_001958"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO335_001958"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6002361.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO335_001959"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO335_001959"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6002362.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO335_001960"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO335_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6002363.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO335_001961"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO335_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR6002364.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO335_001962"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO335_001962"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6002365.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO335_001963"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO335_001963"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6002366.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO335_001964"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO335_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6002367.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO335_001965"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO335_001965"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6002368.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO335_001966"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO335_001966"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6002369.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OO335_001967"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OO335_001967"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6002370.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OO335_001968"
     CDS             21622..22212
                     /locus_tag="OO335_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6002371.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OO335_001969"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OO335_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6002372.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OO335_001970"
     CDS             22684..23379
                     /locus_tag="OO335_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6002373.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO335_001971"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO335_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6002374.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OO335_001972"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OO335_001972"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6002375.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OO335_001973"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OO335_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR6002376.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO335_001974"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO335_001974"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6002377.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO335_001975"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO335_001975"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6002378.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7055 a         7843 c         8277 g         6921 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgattg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcag caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa gagaaaaatc ctgaaattca gggttgactc tgaaagagga aagcgtaata
    30061 tacgccacct cgcgacagtg cgctaaagcg cgtcgc
//
LOCUS       DAJTSF010000041        29799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000041 DAJTSF010000000
VERSION     DAJTSF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.041"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            58..852
                     /locus_tag="OO335_001976"
     CDS             58..852
                     /locus_tag="OO335_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6002379.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            874..2283
                     /locus_tag="OO335_001977"
     CDS             874..2283
                     /locus_tag="OO335_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6002380.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2261..3040
                     /locus_tag="OO335_001978"
     CDS             2261..3040
                     /locus_tag="OO335_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6002381.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3037..3897
                     /locus_tag="OO335_001979"
     CDS             3037..3897
                     /locus_tag="OO335_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6002382.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4045..4620)
                     /locus_tag="OO335_001980"
                     /pseudo
     CDS             complement(4045..4620)
                     /locus_tag="OO335_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4637..4897)
                     /locus_tag="OO335_001981"
     CDS             complement(4637..4897)
                     /locus_tag="OO335_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6002383.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4888..6159)
                     /locus_tag="OO335_001982"
     CDS             complement(4888..6159)
                     /locus_tag="OO335_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6002384.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6237..6599)
                     /gene="queD"
                     /locus_tag="OO335_001983"
     CDS             complement(6237..6599)
                     /gene="queD"
                     /locus_tag="OO335_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6002385.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6918..8717
                     /gene="cysJ"
                     /locus_tag="OO335_001984"
     CDS             6918..8717
                     /gene="cysJ"
                     /locus_tag="OO335_001984"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6002386.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8717..10429
                     /gene="cysI"
                     /locus_tag="OO335_001985"
     CDS             8717..10429
                     /gene="cysI"
                     /locus_tag="OO335_001985"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6002387.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10503..11237
                     /gene="cysH"
                     /locus_tag="OO335_001986"
     CDS             10503..11237
                     /gene="cysH"
                     /locus_tag="OO335_001986"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6002388.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11501..11653
                     /locus_tag="OO335_001987"
     CDS             11501..11653
                     /locus_tag="OO335_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6002389.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11847..12938
                     /locus_tag="OO335_001988"
                     /pseudo
     CDS             11847..12938
                     /locus_tag="OO335_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13084..14121)
                     /gene="iap"
                     /locus_tag="OO335_001989"
     CDS             complement(13084..14121)
                     /gene="iap"
                     /locus_tag="OO335_001989"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6002390.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14373..15281
                     /gene="cysD"
                     /locus_tag="OO335_001990"
     CDS             14373..15281
                     /gene="cysD"
                     /locus_tag="OO335_001990"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6002391.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15283..16710
                     /gene="cysN"
                     /locus_tag="OO335_001991"
     CDS             15283..16710
                     /gene="cysN"
                     /locus_tag="OO335_001991"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6002392.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16710..17315
                     /gene="cysC"
                     /locus_tag="OO335_001992"
     CDS             16710..17315
                     /gene="cysC"
                     /locus_tag="OO335_001992"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6002393.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17365..17688
                     /locus_tag="OO335_001993"
     CDS             17365..17688
                     /locus_tag="OO335_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6002394.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17882..18193
                     /gene="ftsB"
                     /locus_tag="OO335_001994"
     CDS             17882..18193
                     /gene="ftsB"
                     /locus_tag="OO335_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6002395.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18212..18922
                     /gene="ispD"
                     /locus_tag="OO335_001995"
     CDS             18212..18922
                     /gene="ispD"
                     /locus_tag="OO335_001995"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6002396.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18922..19401
                     /gene="ispF"
                     /locus_tag="OO335_001996"
     CDS             18922..19401
                     /gene="ispF"
                     /locus_tag="OO335_001996"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6002397.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19398..20447
                     /gene="truD"
                     /locus_tag="OO335_001997"
     CDS             19398..20447
                     /gene="truD"
                     /locus_tag="OO335_001997"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6002398.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20428..21189
                     /gene="surE"
                     /locus_tag="OO335_001998"
     CDS             20428..21189
                     /gene="surE"
                     /locus_tag="OO335_001998"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6002399.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21183..21809
                     /gene="pcm"
                     /locus_tag="OO335_001999"
     CDS             21183..21809
                     /gene="pcm"
                     /locus_tag="OO335_001999"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6002400.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21949..23088
                     /gene="nlpD"
                     /locus_tag="OO335_002000"
     CDS             21949..23088
                     /gene="nlpD"
                     /locus_tag="OO335_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6002401.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23151..24143
                     /gene="rpoS"
                     /locus_tag="OO335_002001"
     CDS             23151..24143
                     /gene="rpoS"
                     /locus_tag="OO335_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6002402.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24263..24670)
                     /locus_tag="OO335_002002"
     CDS             complement(24263..24670)
                     /locus_tag="OO335_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR6002403.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24817..25410
                     /locus_tag="OO335_002003"
     CDS             24817..25410
                     /locus_tag="OO335_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR6002404.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25410..26357
                     /locus_tag="OO335_002004"
                     /pseudo
     CDS             25410..26357
                     /locus_tag="OO335_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26630..27193)
                     /locus_tag="OO335_002005"
     CDS             complement(26630..27193)
                     /locus_tag="OO335_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002405.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27358..27609
                     /locus_tag="OO335_002006"
     CDS             27358..27609
                     /locus_tag="OO335_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6002406.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27611..29707
                     /locus_tag="OO335_002007"
     CDS             27611..29707
                     /locus_tag="OO335_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6002407.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7295 a         7383 c         8063 g         7058 t
ORIGIN      
        1 attcgtcaca tcatcatcgg caacgaaggt gcattgtgct atatcactga agtaacagtg
       61 aaaatcttta aatacacccc ggaaaacaac ctcttctacg gctatatcct ggaagacatg
      121 aaaaccggct tcaacatcct gcgtgaaatc atggtggaag ggtatcgtcc gtcgatcgct
      181 cgtttgtatg acgctgaaga tggcacccaa cacttcaccc attttgccga cggaaaatgt
      241 gtgctgatct ttatggctga aggtaaccct cgcattgcga aggcgacggg cgaagggatt
      301 gcggaaatcg ttgcccgcta cccgcagtgc cagcgcgtgg acagcaaact gattgaaacc
      361 tggtttaaca acctgaactg gggaccggat aaagtggctg ccgaacgtgt gcagatcctc
      421 aaaaccggca acatgggctt taccaccgaa gtgtccggct gctggagctg catccacgaa
      481 atctacgaaa gcgttattaa ccgtattcgt actgagttcc cgcacgccga cgacatcacc
      541 atgctgggcg gtcactcctc tcatagctat cagaacggca ccaacatgta cttcgtctat
      601 gactacaacg ttgttgactg taagccggaa gaggaaatcg acaagtacca caatccgctc
      661 aacaaaatca tctgcgaaga aaccattcgc ctcggcggtt cgatggtgca ccaccatggc
      721 attggtaaac atcgcgttca ctggagcaag ctggaacacg gcagcgcgtg ggcgttgctg
      781 gaagggctga aaaagcagtt cgatcctaac ggcattatga atacgggtac tatctatccg
      841 attgaaaaat aatgtatcag gcagcgttcc gcgatgacgg ggcgttgcct tttcggcttc
      901 tcaggcgaga agccgttctt attaccggac aatgaagggg taaagatgaa cacttcaccg
      961 gtgcgaatgg atgatttacc gcttaaccgt tttcactgtc gaattgctac gctcactttc
     1021 ggcgcacacc tgaccgacgg ttatgttcta ggcgtcattg gttacgccat tattcaactt
     1081 acgcccgcca tgcaactgac gccgtttatg gcgggaatga tcggcggctc ggcgctcctt
     1141 ggtttgtttc ttggcagcct ggttcttggg tggatctccg accatattgg tcggcaaaaa
     1201 atcttcacct tcagcttttt gctgattacg ctcgcttcgt tcttgcaatt ttttgccacc
     1261 acgccagagc atcttattgg gctgcgcatt ttgatcggca ttggtctggg aggcgattac
     1321 tcagtaggtc acaccttgct ggctgaattt tccccgcgcc gccatcgcgg tattttgctg
     1381 ggcgcattca gcgtggtgtg gaccgtaggc tatgtgttgg caagtattgc cggacatcac
     1441 tttatttccg aaaacccgga ggcctggcgc tggttgctgg catcggcagc tctgcccgcg
     1501 ttgttgatta cgttattacg ctggggaacg ccagaatcgc cacgttggct actgtgccag
     1561 gggcgttttg cagaagctca cgctatcgtg catcgctatt ttggtcccca tgttttactg
     1621 ggcgatgaag tggtaacggc gacccataaa cacatcaaaa ccttgttctc ttcgcgttac
     1681 tggcggcgca cggcgtttaa cagcgtcttc tttgtctgcc tcgtaatccc atggtttgtg
     1741 atttatacct ggctgccaac tatcgcccag actattggtc tggaagatgc gctgactgcc
     1801 agcctgatgc ttaatgcgtt gttaattgtg ggcgcgctgc tgggattagt tctgacgcac
     1861 ctgctggcac atcgcaaatt tttgctggga agttttttgc tgctggcggc aacgctggta
     1921 gtaatggcct gtttgccttc cggcagttca ttaacgctgc tgctttttgt tctcttcagc
     1981 accaccattt cggcagtcag taatctggtg ggcattttgc ctgcggaaag ttttcctact
     2041 gacattcgct cgctgggagt cggttttgcc accgccatga gtcgacttgg cgcggcggta
     2101 agtactggcc tgctgccgtg ggtgctggcg cagtggggaa tgcaagtcac cttattgctc
     2161 ctagcgacag tgttgttggt tggttttgtt gtgacctggc tatgggcacc agaaactaaa
     2221 gccctcccgc tggtggcggc gggaaatgta ggaggtgcga atgaacattc tgttagcgtt
     2281 taaagccgaa ccggatgccg gaatgctggc ggaaaaagag tggcaggcgg cggctcaggg
     2341 taatagcgga ccggatgttt cgctactgcg aagtttactc ggtgctgatg aacaggccgc
     2401 tgccgcgctg ctgttagcgc agagaaaaaa cggtacgccg atgtctttaa ccgcgttgag
     2461 catgggggat gaacgggcgt tgcactggct gcgctatctc atggctcttg ggtttgagga
     2521 agctgtattg ctggagacgg cggcagatct gcgctttgcg ccggaatttg tcgctcgcca
     2581 tattgccgaa tggcagcgtc agaatccgct ggatctgata attaccggca gccaaagcag
     2641 cgaagggcag aatgggcaaa cgccattttt gctggcggaa atgctgggct ggccctgctt
     2701 cacccaggtg gagcgtttca ctctcgacgc gctgtttatc accctcgaac agcgtactga
     2761 acatgggttg cgctgttgcc gggttcgctt gcctgcggtg attgccgtgc gtcagtgcgg
     2821 tgaagtggcc ttgccagtac cgggaatgcg ccagcgaatg gctgcgggga aagcagaaat
     2881 tatccgtaaa actgttgctg ccgaaatgcc agcgatgcag tgtctgcaac tggcaagagc
     2941 agagcaacga cgcggagcga cgctgattga tgggcaaacg gtagcagaaa aagctcaaaa
     3001 gctatggcag gactatttac gccaaaggat gcagccatga atatcgcaat tgtgacaata
     3061 aatcaggaga atgccgcaat agcgagctgg ctggcggcgc aggatttcag cggttgtacg
     3121 ctggcgcact ggcaaataga acctcagccc gtagtggcgg aacaggtgct ggatgcgatg
     3181 gtggaacagt ggcagcgaac gcctgcggac gttgttctct ttccgccggg aacgtttggc
     3241 gatgaactgt cgacccggct ggcctggcgt ttacatggtg caagcatctg tcaggtgact
     3301 tcgttagata ttccaaccgt aagcatgcgc aaatcccact ggggaaatgc gttaacggcg
     3361 acgctgcaaa cagaaaaacg cccgttatgc ctttccctgg cgcgtcaggc gggagcagcg
     3421 aaaaacgcca cgttgccctc tggaatgcag caactgaaca ttgtcccagg tgcgcttccc
     3481 gactggctgg ttagcacaga ggatctgaaa aatgttagcc gcgacccatt agctgaggcc
     3541 agacgggtgc tggtggtggg acagggcgga gaggcagata accaggaaat cgttatgctg
     3601 gcagaaaagc tgggagccga agtgggctac agtcgggcgc gagtgatgaa cggtggcgtt
     3661 gatgctgaaa aagtgattgg tatttccgga catctgctgg cacctgaggt gtgcattgtt
     3721 gttggcgctt ccggcgcagc agcgttaatg gcgggcgtac gcaatagcaa gtttatggtg
     3781 gcgattaatc atgacgccag cgcagcggta ttttcacagg cagatattgg cgtggttgat
     3841 gactggaaag tggtgctgga ggcactggtg acaaacattc acgcagaata ccaataacgt
     3901 gctgccggat gatcctgcct acggttctgg actggggacc tgtaggcctg ataagacgcg
     3961 ttaagcgtcg catcaggcgc tgaatgtcgg atgtggcgtg cggccaacaa atcaaaacaa
     4021 attcaattgg ttacgattat cccgtcaaag caattttttc gctaacgtcc agactccggt
     4081 attcgtggtg gaaagcgcca cgacacccgc gttaatcgca ttacgcgcat cttcttcatc
     4141 gcataccagc ccgccagcaa tcagcggttg gcggattttt tccgtcaccc agcccagcac
     4201 tttgggcatg cagcctggca ggatctcaat acaatccgga ttcgattgcg caacttactt
     4261 atcaatgttg tgaaacgaaa tcgaatcaac aataaacagg tgatgaatac agaaaaaaac
     4321 ttccgctctt gccgctttta gcatcgatgc tttggtgctg ataattccat ccgcctcggt
     4381 caccagtttc aaaaactgaa tcaccacttc tttatttgac gcgccttcca gcaaatcaac
     4441 atgaataaaa gcatatttcc cggcgttctt aatcttttta acgatattac tgatggtgca
     4501 gatattgccg tacaacacgg aaataaactg gcattcagaa tcgatggcca gttgcaggct
     4561 ggcattatct ttaacggcag caatcaccgg attctggcgg agcaggtgta agaggggcat
     4621 aaattatcct tattatttat ccaaagcggt aggtgatgcc gaatccggaa ggcggatagc
     4681 gccactgttg tagtgtggat tcgtcgcaca gcaaacggca ggtgccacac tccaggcagc
     4741 cgcgatagtc aatacgtaag tcaccttcct gtgtgagaga aaaaagacct accggacagg
     4801 cgttaatcaa ccgttgtacc atctgacgct caacggagtc agccggaaca atgtgcggcg
     4861 catcagcaac gcgccagaga ttacgggcta cagacatcgc agactcctga taacatcgcc
     4921 cgccagatgc cacaggccat gacgacgtaa atgatgccag aataactggc gcagcggtgg
     4981 aacaggttta tcaccctgat cccataaatc gcgggaaata tcctgcatta acgcaggcca
     5041 cgcccgatac ctgccagggc gttgcaaaag cgccggaaca tgctgataac gttgtagaac
     5101 atcccatagc aggctgcgct ctacgttgtg atgataaagc gcaaacagat tttgcggctc
     5161 gcggtgctgg caggcgctta ttagcgtttg tgccgccgcc tgtgcgccag tcagcgccat
     5221 atccatgccg cgcacggaaa ttccggtatt gacgcaactg cgcaacgcat cgcccaccag
     5281 cagccagccg ttaccggcgt attgcaccgg catactgtgc aagccacctt ctggcaccag
     5341 atgcgcacca tactccagtg attccgtgtt tttgataagc gtgcgcactg ccggatgcgc
     5401 tttaaagcga gtcagcagct cgcttgccgg aacacgactt tgcgtaaggg aagagagcgg
     5461 gcaaacaatc cctaacgaga gcgtttgttg attagtataa agaaatgcgc cgccgggtaa
     5521 gtcatcacag attccgccgc tgaacagcaa cgctgcgcct tcgttattct ccagatgaaa
     5581 acgttcttca atagcggatg tttccagcga cagcacttct ttgatcccca acgccatcgc
     5641 ttcgccagca ggacgagtca ctaacccgtg acgttcagcc aggacgctgt tggcaccttc
     5701 tgccagcacc acataacggg cgcggagaat atcgtcacca caaataacgc cacagactct
     5761 gccgttttct tcatacagtg catccaccgt cgctccgggg atgcattcga caccttcttt
     5821 ttcggcttcg gcaaccagcc acggatcgaa ccgtgcacgt aatacattcc aggattcacc
     5881 gccgggctgt aagctggaaa acgtcgttgc gccatccggc gttaacaggg aaaggctttc
     5941 gtgagtgatg cgtcgttcaa gaggcgcggt cagatgaaat tgcgggagga gttccgcgag
     6001 tgcatgggta tataaccgcc tgccggaaag atttttgctg ccggggattt cagcgcgttc
     6061 gagtaacaaa acggataaat ccgctcgcgc gcagcgtaac gcgcaagcgg tgcctgcaat
     6121 accggcacca ataataataa tgtcgcagtc gtcttccatg tcatgcccca gatatccaga
     6181 acacaacacc ctaacatagc gttactaaag ggaaattgac cgccgacaca ctcttatcat
     6241 tcgccgcgat agatacaccc tgcggtgcag gtttctttta ccatcaccgc acttaacagc
     6301 ggcacaacgg gtttaacctg atcccaaatc cattttgcta aaacttcgct ggttggattt
     6361 tccagacctg gaatatcatt gagataatgg tgatcgaggc gctcataagt tggcttaaac
     6421 gccgctttca gttcagcgaa atcgataatc cagcccgtat gcggatcgac ttccccggta
     6481 atttccagtc gcaccataaa ggaatgcccg tgcaggcgac cacatttatg cccttccggg
     6541 acatgtggta agcggtgagc ggcttcgaag gtgaaatctt taaataacgt ggtggacatc
     6601 ataatttctc tacaggacga taaaaccgcc gtagagtacc ggaaaggctg ttttttcgca
     6661 ttatctaaaa cgactttgtt gcgcaaaatc gctgatttat cttaatgatt ggctaaattc
     6721 atttgttttt cattaggttg gttaatctat tttgttgtta aagactattg ctaaaacagg
     6781 ttagtcgatt tggttattag ttatcgctat cccgtcttta atccacaccg ttcaccccgt
     6841 taaccttacc ttctcttctg ttttatgggc gctgacaggg cgcagaaaca gctttgctta
     6901 ctggaacata acgacgcatg acgacacagg tcccaccttc cgcgttgctt ccgttgaacc
     6961 cggagcaact ggcacgcctt caggcggcca cgaccgattt aacgcctacc cagcttgcct
     7021 gggtttctgg ctatttctgg ggcgtgctca accagcagcc tgttgcgctt gcagtgacgc
     7081 cagcgcccgc cgcagaaatg ccgggtataa cgattatctc tgcttcgcaa accggcaatg
     7141 cgcgccgggt tgctgaagca ttacgcgatg atttattagc agcaaaactg aacgttaagc
     7201 tggtgaacgc gggcgactat aaattcaaac aaatcgccag cgaaaaactg ctcatcgtag
     7261 tgacgtcaac gcaaggggaa ggggaaccgc cggaagaagc cgtcgcgctg cataagttcc
     7321 tgttctccaa aaaagcgcca aagctggaaa acaccgcgtt tgccgtgttt agcctcggcg
     7381 atagctctta tgaatttttc tgccagtccg ggaaagattt tgacagcaag ctggcggaac
     7441 tggggggtga acgcctgctc gaccgtgtcg attctgatgt tgaataccag gctgctgcca
     7501 gcgagtggcg cgcccgcgtg gttgatgcgc ttaaatcgcg tgcgcctgtc gcggcacctt
     7561 cgcaatccgt cgctactggc gcggtaaatg aaatccacac cagcccgtac agcaaagacg
     7621 cgccgctggt ggctagcctc tctgttaacc agaaaattac tgggcgtaac tctgaaaaag
     7681 acgttcgcca tatcgaaatt gacttaggcg actcgggcct gcgttaccag ccgggtgacg
     7741 cgctgggcgt ctggtatcag aacgatccgg cactggtgaa agaacttgtc gaactgctgt
     7801 ggctgacagg cgatgaacct gtcactgtcg agggcaaaac gttgcctctg aacgaagcgc
     7861 tacagtggca cttcgaactg accgtcaaca ccgccaacat tgttgagaat tacgccacgc
     7921 ttacccgcag tgaaacactg ctgccgctgg tgggcgataa agcgaagtta cagcattacg
     7981 ccgcgacgac gctgattgtt gacatggtgc gtttctcccc ggcacagctt gatgccgaag
     8041 cgctaattaa tctgctgcgc ccgctgaccc cgcgtctcta ttccattgcc tcctcgcagg
     8101 cggaagtcga gaacgaagta cacgtcaccg ttggtgtggt gcgttacgac gtggaaggcc
     8161 gcgcccgtgc cggtggtgcc tccagcttcc tcgctgaccg cgtggaagaa gagggcgaag
     8221 tccgcgtatt tatcgaacat aacgataact tccgcctgcc cgctaacctg gaaaccccgg
     8281 tgattatgat tggcccaggc accggtattg cgccgttccg cgcctttatg cagcaacgcg
     8341 ccgctgacga agcgccaggt aaaaactggc tgttctttgg caatccgcat tttacggaag
     8401 acttcctgta tcaggtggag tggcagcgct acgtcaaaga gggtgtgctg acgcggatcg
     8461 atctcgcctg gtcgcgcgac caaaaagaaa aagtttacgt acaagataaa ctgcgcgaac
     8521 agggcgcgga gctgtggcgc tggatcaatg acggtgccca catttatgtc tgcggcgacg
     8581 ctaatcgcat ggcgaaagac gttgagcagg cacttctgga agtgattgcc gaatttggtg
     8641 gcatggacac cgaagcggcg gatgaatttt taagtgagct gcgcgtagag cgccgttatc
     8701 agcgagatgt ctactaatga gcgaaaaaca tccagggcct ttagtggtcg aaggaaaact
     8761 gacagacgcc gagcgcatga agcttgaaag caactacctg cgcggcacca ttgcggaaga
     8821 tttaaacgac ggtctgaccg gcggctttaa gggcgataac ttcctgctga tccgcttcca
     8881 cggcatgtat cagcaggatg accgcgacat ccgcgccgaa cgtgctgaac agaagctgga
     8941 gccgcgccac gcgatgctgt tgcgctgccg tctgccgggt ggagttatca ccaccaaaca
     9001 gtggcaggcg atcgataaat ttgccggtga aaacaccatc tatggcagca ttcgcctgac
     9061 caaccgccag acgtttcagt tccacggcat tctgaaaaag aacgtcaaac cggtgcacca
     9121 gatgctgcac tcggtcggtc ttgatgcgct ggcgactgct aacgacatga accgtaacgt
     9181 actctgcacc tcgaaccctt acgagtcgca gctgcacgca gaagcgtacg agtgggcgaa
     9241 gaagatttct gaacatctgc tgccgcgtac ccgcgcgtat gcggagatct ggctcgatca
     9301 ggaaaaagtc gccaccaccg atgaagaacc gatccttggt cagacttacc tgccgcgtaa
     9361 gttcaaaacc acggtagtga tcccgccgca gaacgatatt gatctgcacg ccaacgacat
     9421 gaacttcgtg gcgatcgcgg aaaacggcaa gctggtgggc ttcaacctgc tggtgggcgg
     9481 tgggctttct atcgaacacg gtaacaagaa aacctacgcc cgcacggcga gtgagtttgg
     9541 ctatctgccg ctagagcata cgctagcggt ggcggaagcc gtcgtgacaa ctcagcgtga
     9601 ctggggtaac cgaaccgatc gtaaaaatgc caaaaccaaa tacacactgg agcgcgtggg
     9661 ggttgagacg tttaaagcgg aagtggagcg tcgcgcgggg atcaaatttg aaccgatccg
     9721 cccgtatgaa tttacaggac gcggcgatcg tattggctgg gttaagggca ttgatgataa
     9781 ctggcacctg acgctgttta tcgaaaatgg tcgcatcctt gattatccgg ggcgtccgct
     9841 gaaaactggc ctgctggaga tcgcgaagat ccacaaaggt gatttccgca ttacggcgaa
     9901 ccagaatctg atcatcgccg gtgtgccgga aagcgagaaa gcgaagatcg agaaaattgc
     9961 caaagagagc gggttaatga atgccgtcac gccgcagcgt gaaaactcga tggcctgcgt
    10021 gtcattcccg acttgcccgc tggcgatggc ggaagcggag cgtttcctgc cgtcttttat
    10081 cgacaacatc gataatttaa tggtgaaaca tggtgtcagc gatgagcata tcgtgatgcg
    10141 tgtaacaggc tgcccgaacg gttgtggtcg cgcgatgctg gcggaagtgg gcctggtggg
    10201 taaagcaccg ggtcgctaca acctgcatct tggcggcaac cgcattggga cacgtatccc
    10261 acggatgtat aaagaaaaca tcaccgagcc ggaaatcctg gcgtcgcttg atgaactgat
    10321 agggcgctgg gcgaaagagc gcgaagcggg tgaaggcttc ggcgacttta cggtgcgtgc
    10381 gggcatcatt cgcccggtgc tcgatccggc gcgcgatttg tgggattaac catcagcccg
    10441 gtcttgtagg cctgataaga cgcgtgagcg tcgcatcagg caaggcaaac agtgaggaat
    10501 ctatgtccaa actcgatcta aacgccctga acgaactgcc gaaggtagat cgcattctgg
    10561 cgctggcaga aactaacgcc gaactggaaa aactggacgc tgaaggccgc gttgcctggg
    10621 cgctggataa tctgcccggt gaatatgtac tttcttccag ctttggcatt caggcggcgg
    10681 tgagcctgca tctggtgaat cagattcacc cggatattcc ggtgatcctc accgacaccg
    10741 gctatctgtt cccggaaacc taccgcttta ttgacgagtt aacggacaaa ctcaagctca
    10801 acctgaaagt gtaccgcgct accgaaagcg ccgcctggca ggaagcacgc tacggcaaac
    10861 tgtgggaaca gggcgttgaa ggcattgaaa agtacaatga catcaacaaa gtcgaaccga
    10921 tgaaccgggc gctgaaagaa ctgaatgcgc aaacgtggtt tgctggcctg cggcgtgaac
    10981 aatccggcag ccgtgccaat ttaccggtgc tggcaattca gcgtggcgta tttaaagtgc
    11041 tgccgattat cgactgggat aaccgaacta tttatcagta cctgcaaaaa catggcctga
    11101 aatatcaccc attatgggac gaaggatatt tatcggtggg cgatacccat acaacccgta
    11161 aatgggaacc cggcatggcg gaagaagaaa cgcgtttctt tggcttaaaa agggaatgtg
    11221 gattacacga agggtaaata ttgccggata aattaaacgc ctctgtcaga aatgatgggg
    11281 gcgtttttat tttgcgagca gcattccggc atttaaaggt gaacatcgca gggaaaatta
    11341 acaggtatag aatggcgttg ggtgcttgag gctgtctggc gttaagcatt cgcgaggttc
    11401 cagatggaca aaagccccag gcgatatttc tatcaacctg aggctcgcgt tcgaacgtag
    11461 caattcggat gttagtctct tctttgcagg gaggcaagac atgctgacaa aatatgccct
    11521 tgtggcaatc atcgtactgt gttgtacagt actgggattc acgttgatgg taggtgactc
    11581 gttatgtgag ttgagtatca gagaacgtgg tatggagttt aaggcagttc tcgcttacga
    11641 atcgaagaag tagccaccac gcgggggtaa aaccccgcgt actgaattgt gatggtttgg
    11701 tctcctgcac ccttttttta aatttatatt ttttatgtga atatatagtc tctatttatt
    11761 attatgaatt tcaaatgaaa atcattaatt tattcataat gtaaatgttt tattggctga
    11821 tatatatcat agatagtttt taaaatatgc gtaaatatcc tttaagttta ctgaaggata
    11881 aaaatattgt gactttcttt gatttctggg gaaaaaaccg acgtggcgag aaagagggta
    11941 tcgacgacta tcaccttctt tgctggcatt tgctggatgt ggccgcaatg ggctatttaa
    12001 tggttaaaag aaattgcttc gggctggctg attattttcg tcaattaggg atttctgaca
    12061 aggaacaggc agctcgattt ttcgcctggt tgctgtgctg gcacgatatt ggaaaatttg
    12121 cccgctcttt tcaacaactt tacctgcccc ctgaattcaa gattcaggaa ggtgccagaa
    12181 aaaattacga aaaaatctct cattcaacgc tgggctactg gttgtggaat cattatttaa
    12241 gtgaatgtca ggagttactt ccttcatctt cgctctctcc gcgcaaactt aggcgagtga
    12301 tagaaatgtg gatgcccgtg acgacagggc atcatggtcg gccccctgat cgtatggatg
    12361 aattggataa ttttctgcct gaagacaaag ctgcagcacg agattttctc cttgaaatca
    12421 agccactgtt tccgcgaata gagatcccca cattctggga tgatgacgag ggcgttgaac
    12481 ttataaaaca actttcctgg tatatctctg caaccgtcgt acttgcagac tggacagggt
    12541 cgtcaacgcg attttttcca cgcgtcgcac aggcaatgga tattgaagat tactggcaaa
    12601 aggctttagt tcaggctcaa aacgcattaa ccgtctttcc gccacaagca gaaaccgcgc
    12661 ctttcaccgg aattaatacg ctgtttcctt ttattgagaa tacgacaccg ttacagcaaa
    12721 aggtgctgga tctggatatc aatcagccag gaccacagtt gtttattctg gaagatgtga
    12781 ccggcgcagg taaaacagaa gcggcgctta tcctggcgca caggttgatg gctgcgggta
    12841 acgctcaggg gctgtttttt ggcctgccaa caatggcaac ggccaatgcc atgtacgatc
    12901 gactggtcaa aacctggctt gctttctatt cgccagaggt gttccccgct ccagcggaga
    12961 taaaccggcg caatacggtg cggttttccc ggaagtaaac cggacgcact ggatgcgatg
    13021 atgggtatca cttagaattc cccgcccctg cggtagaact cccagctccc attttcaaac
    13081 ccatcaagac gccttcgcca gctccttcac caacggtagc attatccgca taacgtcacg
    13141 gcagcgacgt tctattcttc caggaagtgc cttatcaata tgttgttgat tatccagtct
    13201 tacgtcatgc cagctatttc ccgccgggaa ggcaggtgtt tttgcgcgtt gctgataacc
    13261 atccttattt ccaagattcc agttagtcgc ttccaccgaa agtacagcaa tgcccgcttt
    13321 gtcgaatatt tctgcgtcat tacaaccccc agtgcctttc ggataatttt tattcaaacc
    13381 cggattggtc gttgcggcta ttccgtgact gcgtgcaatt gccagcgccc tgtcgcgcgt
    13441 taatttcctt actgcttcag gggtttttac accgctgttg aaatacaatt tatcgccaac
    13501 aattaagtta tcgagattaa tcaccagcag cgtatttttc ttttcggtgt cactcatccg
    13561 cttgagtaaa ttctcagcgc ctaattttcc ttcctcttcg ccgctggtcg ccacaaatcg
    13621 aataccatac tcggtaggcg tatttttcag gcgttctgcc agttccagca tgacacctaa
    13681 acctgcggcg ttatcatcta ttccttgtaa cgtcagtccg ccgagattgg catctgcgtc
    13741 agcatcgctc agcggggcgt aagtatccag atgcgccata atgatgatct gctgcggcgc
    13801 tttgccttca tgagcggcaa tcaccgtact tcctgtcacg ttatgccagt tcttgcgatt
    13861 atcgcgggcg gtataaatat accgactatt aaatgtccga atatcactgc gataacccat
    13921 ttgctgaaac tgttggcgaa tataatcggc agataacatt tctgctggag ttccggtcat
    13981 gcgtcccgga aagaaagtag caatatgtcg tgcctgggta ttagcaaaat cgccaggttt
    14041 aggtgacgag gcgtgtacgg ggagaataaa gcatacgccg agcgccaggg cagcggtacg
    14101 gtggcgcaat gcggaaaaca tagtgagtcc ttaaatacca tgcaaatttt tttaccgcca
    14161 tagtatgaaa ctgccgctgc gctaaaacaa tttcaaatct tcctaaacgc ccgaaatccg
    14221 gtgccttaag cactttttga tattagcttt gccaaatcgt tattccgtta aggaactact
    14281 cattctaatt ggtaatttca ttcgttctct tacgctccct atagtcgaaa catctgatgg
    14341 caagaaaata gcggtattgc aaaggaacgg ttatggctca aaaacgactt actcacctgc
    14401 ggcaactgga ggcggaaagc atccacatta ttcgcgaggt ggcggcagaa ttctcaaatc
    14461 cggtgatgct ctactctatc ggtaaagatt ccagcgtcat gctgcatctg gcgcgcaagg
    14521 cgttttatcc aggtacgctg cctttcccgt tgctgcatgt cgataccggc tggaaattcc
    14581 gcgagatgta tgagttccgc gatcgtacgg cgaaagccta cggctgcgag ctgctggtgc
    14641 ataaaaaccc ggaaggcgtg gcgatgggga ttaatccatt cgtgcacggc agcgcgaaac
    14701 ataccgatat tatgaaaact gaaggcctga aacaggcgct gaacaaatac ggttttgatg
    14761 ccgccttcgg tggtgcgcgc cgtgacgaag agaaatcccg cgctaaagag cgaatctact
    14821 ctttccgtga ccgcttccat cgctgggatc cgaaaaatca gcgcccggag ctgtggcaca
    14881 actacaacgg gcaaattaac aaaggcgaaa gcatccgcgt cttcccgctc tctaactgga
    14941 ccgagcagga tatctggcaa tacatctggc tggaaaatat cgacattgtg ccgctgtatc
    15001 tcgctgcgga acgtccggtt ctggaacgcg acggtatgtt gatgatgatt gatgacaacc
    15061 gtatcgacct gcaaccgggc gaagtgatca aaaaacggat ggtgcgtttc cgtacgctgg
    15121 gctgctggcc gctgaccggt gcggtggagt caaatgcaca aacactgccg gaaatcatcg
    15181 aagagatgct ggtttccacc accagtgaac gtcagggtcg cgtgattgac cgcgaccagg
    15241 cgggatccat ggagctgaaa aaacgtcagg ggtattttta agatgaacac cgtacttgca
    15301 caacaaatcg ccaatgaagg cggcgtcgaa gcctggatga ttgcgcaaca acataaaagc
    15361 ctgctgcgtt ttctgacctg tggtagcgtc gatgacggca aaagtaccct gattggtcgt
    15421 ctgctgcacg atacccgcca aatctacgaa gatcagctct catcgctgca taacgacagt
    15481 aagcgtcacg gaacccaggg cgaaaagctg gatctggctc tgctggtgga cggcctgcaa
    15541 gctgagcgcg aacagggcat cactattgat gtggcctacc gctatttctc taccgagaag
    15601 cgtaaattta ttatcgccga caccccaggg cacgagcagt acactcgcaa tatggcgact
    15661 ggcgcatcga catgtgaact ggcgatctta ctgattgatg cccgtaaagg cgtgctcgat
    15721 caaacccgtc gtcacagttt tatctccaca ctgttgggga tcaaacatct ggtcgtggcg
    15781 atcaacaaaa tggatctggt ggattacagt gaagagacgt tcacccgtat tcgtgaagat
    15841 tatctgacct ttgccgggca gctgccgggt aatctggata tccgctttgt gccgctctcc
    15901 gcactggaag gtgacaacgt ggcttcgcaa agtgaaagta tgccgtggta cagcggtctg
    15961 acactgctcg aagtgctgga aaccgtggag atccagcgag tggtggatgc ccagccaatg
    16021 cgcttcccgg tgcagtacgt taaccgccca aatctcgatt ttcgtggcta cgccggaatg
    16081 ctggcatccg gtcgcgtgga agtcgggcaa cgtgtaaaag tgctgccctc tggtgtggaa
    16141 tcaaacgtcg cgcggatcgt gacttttgat ggtgatcgcg aagaagtctt tgccggagaa
    16201 gcgatcaccc tggtgctgac ggatgagatc gatatcagcc gtggtgatct gctgctggcg
    16261 gcagacgaag cgttaccggc ggtgcagagt gcgtcggtgg atgtggtatg gatggcggaa
    16321 cagccgcttt ccccgggcca gagttatgac atcaaaattg ccggtaagaa gacgcgtgct
    16381 cgtgttgatg gcattcgtta tcaggttgat attaataacc ttacccaacg cgaagttgaa
    16441 aacctgccgc tgaacggcat cggcctggtg gatctcactt ttgacgagcc actggtgtta
    16501 gatcgctatc agcaaaatcc ggttaccggt gggctgattt ttatcgatcg cctgagcaat
    16561 gtgaccgtgg gtgccggtat ggtgcacgag ccagttagcc aggcaacagc tgcgtcatct
    16621 gaattcagtg cattcgaact ggaattgaat gccctggttc gtcgccactt tccgcactgg
    16681 ggcgcgcgcg atttgctggg ggataaataa tggcgctgca tgacgaaaac gtcgtctggc
    16741 atagtcatcc ggtcactgtg caacaacgcg aactacacca cggtcatcgt ggtgtagtgc
    16801 tgtggtttac cggcctctcc gggtccggta aatcaacggt cgccggggcg ctggaggagg
    16861 cgttacataa actcagcgtc agtacgtatc tgctggatgg cgacaatgtt cgccacggat
    16921 tatgcagcga tctcggtttt agcgatgccg atcgtaaaga gaatatccgt cgcgtcggtg
    16981 aagtggcgaa tttgatggtt gaagccggac tggtggtgct gaccgcattt atctcgccac
    17041 accgcgccga acgccagatg gttcgcgaac gcgtaggaga agggcgcttt attgaagtgt
    17101 ttgtcgatac gccgctggcg atttgcgaag cccgcgatcc aaaaggctta tataagaaag
    17161 cgcgtgccgg tgaactgcgc aactttacgg gaatagattc cgtttacgaa gcgcctgaat
    17221 cggcagaaat tcatctcaat ggtgaacaat tagtaacaaa tttggtacag caattattag
    17281 atctgttgag acagaacgat attatcagat cctgagacac caccgggttt ccatgcccgg
    17341 ttcgtcaaag taacaggatc aaatatgcgt aatagccata acattacact aacaaataac
    17401 gacagcctta ctgaggatga agaaactaca tggtcactgc ctggtgccgt ggtcggtttt
    17461 atctcctggt tatttgcgct ggcgatgccg atgttgattt atggctctaa cacgctgttc
    17521 ttctttctct acacctggcc tttctttctg gcactgatgc ctgtcgcggt agtggtgggg
    17581 attgcgctgc attcattgat ggacggaaag ctacgctaca gtattgtttt caccctggtg
    17641 acagtaggca ttatgtttgg cgcactgttt atgtggctac tgggctaatt tgtactttct
    17701 tgtctcctct gttcataatt caaaccgtaa ctaataatga gattatgttc tgcacgccct
    17761 gggtatacgt aacaatgggc aaatgtggta catttgcccg cgttgtcgcg gtatccccaa
    17821 cagaggatgt agagtcgtct tcggatgcat gggatgatga tgcagttttt cagggggcag
    17881 gatgggtaaa ctaacgctgc tgttgctggc tattctggtc tggctacagt attcgctgtg
    17941 gttcggtaag aacggtatac atgactatac ccgcgtcaat gatgatgtgg cggcactgca
    18001 agctacaaac gcgaaactta aagcgcgaaa cgatcaactt tttgccgaaa ttgacgatct
    18061 caatggcggc caggaggcgc tcgaagagcg tgcgcgtaat gaactcagca tgaccaggcc
    18121 gggcgaaact ttttatcgtc tggtgcctga cgcgtcgaag cgcgcacagt ctgcggggca
    18181 aaacaatcga taaatcagcc cgggaattaa catggcaacc actcatttgg atgtttgcgc
    18241 cgtggttccg gcggccggat ttggccgtcg aatgcaaacg gaatgtccta agcaatatct
    18301 ctcaatcggt aatcaaacca ttcttgaaca ctcggtgcat gcgctgctgg cgcatccccg
    18361 ggtgacacgt gtcgtcattg ccataagtcc tggcgatagc cgttttgcac aacttcctct
    18421 ggcgaatcat ccgcaaatca ccgttgtaga tggcggtgat gagcgtgccg attccgtgct
    18481 ggcaggtctg aaagccgctg gcgatgtgca gtgggtattg gtgcatgacg ccgctcgtcc
    18541 ttgtttgcat caggatgacc ttgcgcgatt gttggcgttg agcgaaacca gccgcacggg
    18601 agggatcctc gccgcaccag tgcgcgatac tatgaaacgt gccgaaccgg gcaaaaatgc
    18661 cattgctcat accgttgatc gcaacggctt atggcacgcg ctgacgccgc aatttttccc
    18721 tcgtgagctg ttacatgact gtctgacgcg cgctctaaat gaaggcgcta ccattaccga
    18781 cgaagcctcg gcgctggaat attgcggatt tcatccgcag ttggtcgaag gccgcgcgga
    18841 taacattaaa gtcacgcgcc cggaagattt ggcactggcc gagttttacc tcacccgaac
    18901 catccatcag gagaatacat aatgcgaatt ggacacggtt ttgacgtaca cgcctttggc
    18961 ggtgaaggcc caattatcat tggtggcgta cgcattcctt acgaaaaagg attgctggcg
    19021 cattctgatg gcgacgtggc gcttcatgcg ttgaccgatg cattgcttgg cgcggcggcg
    19081 ctgggggata tcggcaaact gttcccggat accgatccgg catttaaagg tgccgacagc
    19141 cgcgagctgc tacgcgaagc ctggcgtcgt attcaggcga agggttatac ccttggcaac
    19201 gtcgatgtca ctatcatcgc tcaggcaccg aagatgttgc cgcacattcc acaaatgcgc
    19261 gtgtttattg ccgaagatct cggctgccat atggatgatg ttaacgtgaa agccactact
    19321 acggaaaaac ttggatttac cggacgtggg gaagggattg cctgtgaagc ggtggcgcta
    19381 ctcattaagg caacaaaatg attgagtttg ataatctcac ttacctccac ggtaaaccgc
    19441 aaggcaccgg gctgctgaaa gccaatccgg aagactttgt ggtggtggaa gatttgggct
    19501 ttgagcctga tggtgaaggt gagcatattc tggttagaat cctcaaaaac ggctgcaata
    19561 cccgttttgt ggcggatgca ctggcgaaat tcctgaaaat tcattcccgt gaagtcagct
    19621 tcgctgggca aaaagacaaa catgctgtaa cggaacagtg gttatgtgct cgcgtgccgg
    19681 gcaaggaaat gcccgatctg agcgcctttc aactggaagg ctgccaggtg ctggagtatg
    19741 cgcggcacaa gcgcaagctg cgtttagggg cgctgaaagg taacgccttt accctggttc
    19801 tgcgcgaagt gagcaatcgc gatgacgttg aacaacgtct gatcgatatt tgcgtaaaag
    19861 gtgtaccgaa ctacttcggt gcccaacgtt ttgggattgg cggtagcaac ttgcagggcg
    19921 cgctgcgctg ggcgcaaacc aatactccgg tgcgcgatcg caataaacgg agtttttggt
    19981 tgtcggcagc ccgcagtgcg ttgtttaatc agattgttgc tgagcgcctc aaaaaagcag
    20041 acgttaatca agttgttgac ggcgatgcgc tacaattagc cggacgtggt agctggtttg
    20101 tagcaaccac cgaagaactg gcggaattac agcgtcgcgt caacgataaa gagctgatga
    20161 taaccgccgc attgccgggc agtggcgaat ggggaactca gcgtgaagcg ctggcattcg
    20221 aacaagcagc tgtcgccgca gaaactgaat tacaagcttt actggtgcgc gaaaaagttg
    20281 aagccgcgcg cagagcgatg ctgctgtatc cgcaacaatt aagctggaac tggtgggatg
    20341 acgtcaccgt agagatccgt ttctggcttc cggcgggtag ttttgcaacc agcgttgtca
    20401 gggaacttat caacacaaca ggtgattatg cgcatattgc tgagtaatta tgacggggta
    20461 catgcacccg gtatacaaac gctggcgaaa gccttgcgtg agtttgctga cgttcaggtg
    20521 gtcgcccccg atcgtaaccg cagcggcgct tcaaattctc tgacactgga atcctccctg
    20581 cgcacgttta cctttgaaaa tggtgatatt gctgtgcaaa tgggaacccc gaccgattgt
    20641 gtctatcttg gcgtgaatgc tctgatgcgt ccgcgcccgg acattgttgt gtccggaatt
    20701 aacgccgggc cgaatctggg ggatgatgtt atttattccg gtacggtagc cgccgcgatg
    20761 gaaggccgtc atttaggttt tccggcgctg gccgtctcgc ttgacgggca taaacattac
    20821 gacactgccg cggcggtaac ctgttcaatt ttgcgcgcac tgtgtaaaga gccgctgcgc
    20881 accggacgta ttcttaatat taacgttcag gatttaccct tggatcaaat caaaggtatt
    20941 cgcgtgacgc gctgcggtac acgacatccg gcagatcagg tgatcccgca gcaagatccg
    21001 cgcggcaata cgctgtactg gattggcccg ccgggcggta aatgtgatgc cggtccgggg
    21061 accgattttg ctgcggtaga tgagggctat gtctccatca cgccgctgca tgtggattta
    21121 actgcgcata gcgcgcaaga tgtggtttca gactggttaa acagcgtggg agttggcacg
    21181 caatggtaag cagacgcgta caagcacttc tggatcaatt acgtgcgcaa ggtattcagg
    21241 atgagcaggt gctgaatgca cttgccgccg tgccgcgtga aaaattcgtt gatgaagcgt
    21301 ttgaacaaaa agcctgggac aatatcgcct tgccgatagg tcaggggcag acaatttcgc
    21361 agccgtatat ggtggcgcga atgaccgaat tactcgagct gacgccgcag tcgcgggtgc
    21421 tggaaattgg cactggttcg ggatatcaaa cggcaatcct ggcgcatctt gtccagcatg
    21481 tttgctcggt tgaacggatt aaaggcttgc agtggcaggc acgtcgccgc ctgaaaaatc
    21541 ttgatttaca taatgtttca acccgtcatg gtgatggatg gcaaggttgg caggcacgtg
    21601 cgccgtttga cgctatcatt gttacggcgg caccgccgga aattccaact gcgctaatga
    21661 cgcagctgga cgaaggcggg attctcgtct tacccgtagg ggaggagcac cagtatttga
    21721 aacgggtgcg tcgtcgggga ggcgaattta ttatcgatac cgtggaggcc gtgcgctttg
    21781 tccctttagt gaagggtgag ctggcttaaa acgtgaggaa atacctggat ttttcctggt
    21841 tattttgccg taggtcagcg tatcgtgaac atcttttcca gtgttcagta gggtgccttg
    21901 cacggtaatt atgtcactgg ttattaacca atttttcctg ggggataaat gagcgcggga
    21961 agcccaaaat tcaccgttcg ccgcattgcg gctttgtcac tggtttcgct atggctggca
    22021 ggctgttctg acacttcaaa tccaccggca ccggtcagct ccgttaatgg caatgcgcct
    22081 gcaaatacta attctggtat gttgattacg ccgccgccga aaatggggac gacgtctaca
    22141 gcgcagcaac cgcaaattca gccggtacag cagccacaaa ttcaggctac tcaacaaccg
    22201 caaatccagc cagtgcagcc agtagctcag cagccggtac agatggaaaa cggacgcatc
    22261 gtctataacc gtcagtatgg gaacattccg aaaggcagtt atagcggcag tacctatacc
    22321 gtgaaaaaag gcgacacact tttctatatc gcctggatta ctggcaacga tttccgtgac
    22381 cttgctcagc gcaacaatat tcaggcacca tacgcgctga acgttggtca gaccttgcag
    22441 gtgggtaatg cttccggtac gccaatcact ggcggaaatg ccattaccca ggccgacgca
    22501 gcagagcaag gagttgtgat caagcctgca caaaattcca ccgttgctgt tgcgtcgcaa
    22561 ccgacaatta cgtattctga gtcttcgggt gaacagagtg ctaacaaaat gttgccgaac
    22621 aacaagccaa ctgcgaccac ggtcacagcg cctgtaacgg taccaacagc aagcacaacc
    22681 gagccgactg tcagcagtac atcaaccagt acgcctatct ccacctggcg ctggccgact
    22741 gagggcaaag tgatcgaaac ctttggcgct tctgaggggg gcaacaaggg gattgatatc
    22801 gcaggcagca aaggacaggc aattatcgcg accgcagatg gccgcgttgt ttatgctggt
    22861 aacgcgctgc gcggctacgg taatctgatt atcatcaaac ataatgatga ttacctgagt
    22921 gcctacgccc ataacgacac aatgctggtc cgggaacaac aagaagtgaa ggcggggcaa
    22981 aaaatagcaa ccatgggtag caccggaacc agttcaacac gcttgcattt tgaaattcgt
    23041 tacaagggga aatccgtaaa cccgctgcgt tatttgccgc agcgataaat cggcggaacc
    23101 aggcttttgc ttgaatgttc cgtcaaggga tcacgggtag gagccacctt atgagtcaga
    23161 atacgctgaa agttcatgat ttaaatgaag atgcggaatt tgatgagaac ggagttgagg
    23221 tttttgacga aaaggcctta gtagaagagg aacccagtga taacgatttg gccgaagagg
    23281 aactgttatc gcagggagcc acacagcgtg tgttggacgc gactcagctt taccttggtg
    23341 agattggtta ttcaccactg ttaacggccg aagaagaagt ttattttgcg cgtcgcgcac
    23401 tgcgtggaga tgtcgcctct cgccgccgga tgatcgagag taacttgcgt ctggtggtaa
    23461 aaattgcccg ccgttatggc aatcgtggtc tggcgttgct ggaccttatc gaagagggca
    23521 acttggggct gatccgcgcg gtagagaagt ttgacccgga acgtggtttc cgcttctcaa
    23581 catacgcaac ctggtggatt cgccagacga ttgaacgggc gattatgaac caaacccgta
    23641 ctattcgttt gccgattcac atcgtaaagg agctgaacgt ttacctgcga accgcacgtg
    23701 agttgtccca taagctggac catgaaccaa gtgcggaaga gatcgcagag caactggata
    23761 agccagttga tgacgtcagc cgtatgcttc gtcttaacga gcgcattacc tcggtagaca
    23821 ccccgctggg tggtgattcc gaaaaagcgt tgctggacat cctggccgat gaaaaagaga
    23881 acggtccgga agataccacg caagatgacg atatgaagca gagcatcgtc aaatggctgt
    23941 tcgagctgaa cgccaaacag cgtgaagtac tggcacgtcg attcggtttg ctggggtatg
    24001 aagcggcaac actggaagat gtaggtcgtg aaattggcct cacccgtgaa cgtgttcgcc
    24061 agattcaggt tgaaggcctg cgccgtttgc gcgaaatcct gcaaacgcag gggctgaata
    24121 tcgaagcgct gttccgcaag taagtaagca tctgtcagaa aggccagtct caagcgaggc
    24181 tggccttttt cttttgtttg ggaagcttgt gtttttgtcg cctggataag acacgtcagc
    24241 gtcctacccg gcaatcagca ttctatgtgc tcatcatctt gcgcaccaac tgcataaaca
    24301 gctcctgctc ttctccactc aaacgcccca aaaattcctc atccacgctg tcaccaatag
    24361 gtatcgccgc agcaagtagc ttttctcctt cggcagtcag ccagacaaag cgtcgccgct
    24421 tgtcagcgac gtcatgttca cgtctgacta agccacgatt ttccattctt gccaacattt
    24481 ctgctagcgt tgctttggtg ctgactgctg cttctatcag tgccacctgt tcaataccag
    24541 gtttatcagc aatggcgcgc atcaccgcgt actgtggttt ggtcaggtca ggtaactcgt
    24601 gctgccagcg agccgtgtgc tgctgaaaaa gctgtcgtaa ctgatggaac gctttatttc
    24661 gtaacgccat gtaaactccg ggctaattct ctgtcgctat catagcggtt ttttagtgac
    24721 gaagaagatg ttgttacttt tcaatccttg ccgctgtgga aatgaaggag tatgttaata
    24781 ataacgttcg tatacgaata attaagagaa taagcaatga aactgatcgt cgggatgaca
    24841 ggggctaccg gtgcgcctct tggtgtggca ttactgcaag cgctgcgtga gatgccgaat
    24901 gtcgagactc atctggtgat gtcgaagtgg gcgaaaacca ccattgaact ggaaacgcct
    24961 tacagcgctc gcgatgttgc tgccctcgca gacttcagcc ataacccggc ggatcaggcg
    25021 gcgatcatct catccggttc ttttcgtacc gacggcatga tcgttattcc gtgcagtatg
    25081 aaaacgctcg ccggtatccg cgctggttac gccgatggcc tggtagggcg cgcggcggac
    25141 gtcgtgctca aagaaggccg caaactggtg ctggtgccgc gtgaaatgcc gcttagcacc
    25201 atccatctcg aaaatatgct cgcactttca cgcatgggcg tggcgatggt gccgccgatg
    25261 cctgcctttt ataaccatcc cgaaacggta gatgacattg tccaccatgt ggtagcccgc
    25321 gtgctggatc aatttggcct cgaacatccc cacgccaggc gctggcaagg attgccgcag
    25381 gcccggaatt tttctcagga gaatgaataa tggcatttga tgatttacgc agctttttac
    25441 aggcgcttga tgaccacggc cagttactga aaatcagcga agaagtgaac gctgagccgg
    25501 atctggcagc agcagctaac gccaccgggc gtatcggcga cggcgcgccc gcgctgtggt
    25561 ttgataatat tcgcggcttt accgatgccc gcgtggcgat gaataccatc ggttcctggc
    25621 agaaccacgc gatttccctt ggcctgccac caaacacccc ggtcaaaaag cagattgatg
    25681 agtttatccg ccgctgggat aacttcccga ttgccccgga gcgccgcgcc aatccagcct
    25741 gggcgcagaa caccgtcgat ggcgaagaga tcaacctgtt cgatattctg ccgctgtttc
    25801 gcctgaatga tggcgatggc ggtttctatc tcgacaaagc gtgcgtggtt tcccgcgatc
    25861 cgctcgaccc ggataacttc ggcaagcaga acgtcggcat ctaccgcatg gaagtgaagg
    25921 gcaagcgtaa gctcggcctg caaccggtgc cgatgcacga tatcgccctg catctgcata
    25981 aagcagaaga gcgcggtgaa gatctgccga ttgcgatcac gctcggtaac gatccgatca
    26041 tcacgctgat gggggccacg ccgctgaaat acgatcagtc tgagtatgaa atggcaggcg
    26101 cgctgcgtga aagcccgtac ccgatcgcca ccgctccgct gaccggtttt gatgtgccgt
    26161 ggggttcaga agtgatcctc gaaggggtta tcgaaagccg taaacgcgaa atcgaagggc
    26221 cgttcggtga gtttaccggg cactactccg gtgggcgcaa catgaccgtg gtgcgcatcg
    26281 ataaagtctc ttatcgcagc aaaccgattt tcgaatcact ctatctcggt atgccgtgga
    26341 ccgaaatcga ctacctatga ctaggtaatt tattcaagat aaccgggaag agattaaaaa
    26401 agacgggaat agattggaat caacaagtta ggaactcaag gttttcaaga taaagcgagt
    26461 ccaaaaatgt taaacggttc gatatcaata aaacattcaa gataactcac aggttcttac
    26521 aatagccttt aaagaccgtt caaaaacgat tcaggtgttt attatagcgc tgatgggtgc
    26581 aagcgccaaa accaatgtaa ttttagcaca gctaggcact tgcactgggt tatgcgctgg
    26641 ctgctttctg aggtagattt aagtgatcgc ccttagcttc ggacaacgcg taaatctctc
    26701 taaccttttc gtctggcaaa ttaccaaggc ttaacgctat ggctctacga actccaacta
    26761 gagcgtgcaa ctcttggatg tgctcatcca gaagaatgag taattgctcc gctccttttc
    26821 tcccaagaac atcagctact tgttgttttt cttgtggttt aagaaatgac aagaacatta
    26881 gaatttgagc atcaacatta tccgcattct cttttttggg ttctttggaa tcttttagtt
    26941 caacatcacc ctctcccgtc atgagccatt ccaaggtaac attttcgctt tctgctatct
    27001 tttttaactg atacgggcgt ggtatcggtg cgtaatcccg cctgttgtaa taatttttta
    27061 aagtgccttc attgatcccc catgcccgag cagccgcatt tcttgaaggg tatcgttcta
    27121 ctaacctaaa gattctttcg ataatcggtt cttttccatc ggcaggaaaa gaaccttggg
    27181 gtgcttttct cattcttggt tcctttctat ctaattcatt gataaataat aaaattaaaa
    27241 aataaaccat aaaaacacca aagaaccctt tgcagggagt ctttggtatc ttaatcttta
    27301 tccatacgga tatccgaccg gatatccgcg cgggtaattt tgtaggttaa aggaaagatg
    27361 agacaaagag aagatagtga ccgcgattgg ccttcgctga agattcagct ggcgctcaaa
    27421 gataacgggt ggtcttcctt ccgggagctt tcactccatg ctggttatgc tcaggattca
    27481 ctgaaagcgg tagttcgcac accaaacaaa aaatacgaac gcgtaatttc tgacgctata
    27541 ggtgtaccgc cagaaatcat ctggcccagc cggtatcgta acgatggatt tatcaggaag
    27601 gcgtcgtgat atgcagtcgt acgttaccgt aaatgacctg ctcggcgtac caggaatgcc
    27661 agccaccaca aaaggcatcc gacaggcgtt acagcgtttt tctcgtgatc ttggtgacgt
    27721 tagtcgccgt cgggaaggca caaaagccat tgagtaccac attgattgtc tgcctgaaat
    27781 aacacgaaag gcactgcgtg aacgctatgt ggaacagctg gtggctacag agaacaatgt
    27841 ttctgaagtg aaagctgtta ccagaaaaac acgcaatcct gacgctgtcc aggcaatcga
    27901 agcatatcgc ggttctccac aactgatgga agaacgcctg aatgcgctga ccgaaaacca
    27961 gcgctgggta tctgaagcaa gagctgcgct ggtggtggaa gtgctgaagc tggaaagcgc
    28021 cggtaacccc gggcgactga aagccattaa ctttcttgtt gaaaaagccc gtaaaggtga
    28081 gctgccggag cgcctgcaac aggccgcagt taacgccaat gcaaaacgtg gcgctaatcg
    28141 tactatcagc cgcgatccac tttaccagtg ggttctgaaa tacaaccagt cgcagaacgc
    28201 cgccgaacgg ctgctgttac tggcaccggg aaaacgcgac gaaataaaac cggaagaaat
    28261 cagctggctg cctgaatttc tggcgcagta tcgccaggtg aacggcagac ccatgtcaga
    28321 agcctacgag gattttgtcg cagaatggca gcgtcgccat gcggacgaac cgtacatgct
    28381 ggaggtgatg ccttcctatg atgttgtccg gtatgccatg aaaaaactgc cggaagtggt
    28441 gaaacaaaaa gggcgcgtta ccggcagtga ataccgtcag cttgaaggat tcacccgccg
    28501 cgactggaca gctatgccgg tgaattatgt ctggattggt gacggtcacg gtatgaagct
    28561 gaaatgtgcg catccgatcc acgggcgtcc attctcaccg gaagtcacgt ttgtgatcga
    28621 tggtggaaca cggtttgttg ttggctggag tcttgacctt gccgaaaacg tgttcgccgt
    28681 tgccggtgcg atacagcacg gcattcgcaa ccacggaaaa ccgttcctgt attactcgga
    28741 taacggttcc ggtgaaacgg ctgacatgct ggacaaagag gtcgtgggta ttctgccacg
    28801 cctggggatt aaacacccta ccggtattgc cggtaatcca cagggacgcg gcattattga
    28861 acgcctgaac cggacactgc cgatgcgtat agccaggagg tatcgcacct attttggcaa
    28921 gggtgctgac cgggaatcgt tgcgtgtact taaccgtgat ctgcgttcag cgtttaacgc
    28981 cctgcaacag gacaagcctc tgaacgaccg ccagaaagcg gcaatgcgcg agctgccatc
    29041 gtgggcggag cttattgagg ctattcgtga aggcgtggaa tggtacaaca accgcccgca
    29101 ttctgagctg ccgatgaaac cgaacggacg gcattacagc ccgacagagt tcagaaaaaa
    29161 acgccaggca gaagaagaca ccgaaattga gtggctgagt gatcttgagc tgcgcgacat
    29221 gttccgccca atggtggaac gaccggtaag acgctgcgaa attcagtggc tgaacaatat
    29281 ttactacgcg ccggagttgc gtgacgaaca cggtcgcaaa gtgcttatca gttatgacat
    29341 tcatgacgcc gagcgaatta cggtacgtcg caaggacggc agctttatct gtgaggcgat
    29401 atggaacggc aataaacgcg cagcctttgc tgtcagtgct gaatatcaca aacaacagca
    29461 gcgtattaaa ggaatgcgca aacgcgctga ggaaaaaatt cgtgatgctg aagatgaagg
    29521 tattcagatt ctggaacaca aacaggctga accctggctg agtaatgttt atcgcccggt
    29581 tggtaatgtg gtggcagttc agcaaccgga atatgaagaa gagcatgacg aagaatttga
    29641 acgcgatttc cggctgggga tgcaaaaact ttttgccatg caggaagaag atgatccgct
    29701 ggcctgaata aaaaaacagt ccgcgggaac ggactgttaa ataaaggaaa caaagctgat
    29761 ttgaaataac cgaggaaata ctatgactga tattgatga
//
LOCUS       DAJTSF010000042        29726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000042 DAJTSF010000000
VERSION     DAJTSF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.042"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..441)
                     /locus_tag="OO335_002008"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="OO335_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO335_002009"
     CDS             complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO335_002009"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR6002408.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO335_002010"
     CDS             complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO335_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR6002409.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO335_002011"
     CDS             complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO335_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR6002410.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO335_002012"
                     /pseudo
     CDS             complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO335_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5754..6257
                     /locus_tag="OO335_002013"
     CDS             5754..6257
                     /locus_tag="OO335_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6002411.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6337..6588)
                     /locus_tag="OO335_002014"
     CDS             complement(6337..6588)
                     /locus_tag="OO335_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6002412.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO335_002015"
     CDS             complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO335_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6002413.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7052..7375
                     /locus_tag="OO335_002016"
     CDS             7052..7375
                     /locus_tag="OO335_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6002414.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7546..8043
                     /gene="ftnA"
                     /locus_tag="OO335_002017"
     CDS             7546..8043
                     /gene="ftnA"
                     /locus_tag="OO335_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6002415.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8080..8319)
                     /locus_tag="OO335_002018"
     CDS             complement(8080..8319)
                     /locus_tag="OO335_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6002416.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8509..9720
                     /gene="tyrP"
                     /locus_tag="OO335_002019"
     CDS             8509..9720
                     /gene="tyrP"
                     /locus_tag="OO335_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6002417.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRNLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO335_002020"
     CDS             complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO335_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6002418.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10643..10729)
                     /locus_tag="OO335_002021"
     tRNA            complement(10643..10729)
                     /locus_tag="OO335_002021"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10693..10695),aa:Leu,seq:taa)
     gene            complement(10742..10815)
                     /locus_tag="OO335_002022"
     tRNA            complement(10742..10815)
                     /locus_tag="OO335_002022"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10781..10783),aa:Cys,seq:gca)
     gene            complement(10870..10945)
                     /locus_tag="OO335_002023"
     tRNA            complement(10870..10945)
                     /locus_tag="OO335_002023"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10910..10912),aa:Gly,seq:gcc)
     gene            complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO335_002024"
     CDS             complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO335_002024"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6002419.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO335_002025"
     CDS             complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO335_002025"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6002420.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO335_002026"
     CDS             complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO335_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6002421.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14483..14659
                     /locus_tag="OO335_002027"
     CDS             14483..14659
                     /locus_tag="OO335_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002422.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14646..14870
                     /locus_tag="OO335_002028"
     CDS             14646..14870
                     /locus_tag="OO335_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6002423.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO335_002029"
                     /pseudo
     CDS             complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO335_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO335_002030"
     CDS             complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO335_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6002424.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO335_002031"
     CDS             complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO335_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6002425.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO335_002032"
     CDS             complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO335_002032"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6002426.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO335_002033"
     CDS             complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO335_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6002427.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO335_002034"
     CDS             complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO335_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6002428.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO335_002035"
                     /pseudo
     CDS             complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO335_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO335_002036"
     CDS             complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO335_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6002429.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22563..23969
                     /gene="fliD"
                     /locus_tag="OO335_002037"
     CDS             22563..23969
                     /gene="fliD"
                     /locus_tag="OO335_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6002430.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23994..24404
                     /gene="fliS"
                     /locus_tag="OO335_002038"
     CDS             23994..24404
                     /gene="fliS"
                     /locus_tag="OO335_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6002431.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24404..24769
                     /gene="fliT"
                     /locus_tag="OO335_002039"
     CDS             24404..24769
                     /gene="fliT"
                     /locus_tag="OO335_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR6002432.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24847..26334
                     /gene="amyA"
                     /locus_tag="OO335_002040"
     CDS             24847..26334
                     /gene="amyA"
                     /locus_tag="OO335_002040"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6002433.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26368..26781)
                     /locus_tag="OO335_002041"
     CDS             complement(26368..26781)
                     /locus_tag="OO335_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6002434.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26968..28173
                     /gene="yedE"
                     /locus_tag="OO335_002042"
     CDS             26968..28173
                     /gene="yedE"
                     /locus_tag="OO335_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6002435.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28170..28403
                     /gene="yedF"
                     /locus_tag="OO335_002043"
     CDS             28170..28403
                     /gene="yedF"
                     /locus_tag="OO335_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6002436.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28510..29180
                     /locus_tag="OO335_002044"
                     /pseudo
     CDS             28510..29180
                     /locus_tag="OO335_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(29220..>29726)
                     /locus_tag="OO335_002045"
     CDS             complement(29220..>29726)
                     /locus_tag="OO335_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6002437.1"
                     /translation="IQRLHSCIANIRRDYLHKVTTTVSKNHVMIVIEDLKVSNMSKSA
                     AGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCAC
                     CGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEP
                     TEMIQATA"
BASE COUNT         7386 a         7678 c         7079 g         7583 t
ORIGIN      
        1 caatgcgatt attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat
       61 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
      121 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat
      181 aatgaaaagc gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
      241 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt
      301 ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat
      361 gccaacggca aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga
      421 tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg
      481 cggcggttat catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg
      541 cctgagttgc acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga
      601 agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag
      661 cttcctgcat aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga
      721 caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta
      781 atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc
      841 ctttcgcctc cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa
      901 tatcacgcgc aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc
      961 gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct
     1021 ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct
     1081 gtagtccttg cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg
     1141 ccagcgttcc atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg
     1201 ttaacggttc tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc
     1261 gctttacaga catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca
     1321 gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag
     1381 catcagacag tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg
     1441 attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt
     1501 tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct
     1561 ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac
     1621 gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc
     1681 aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc
     1741 gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc
     1801 agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac
     1861 cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct
     1921 gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca
     1981 tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac
     2041 aaacccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg
     2101 acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag
     2161 aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata
     2221 ttaatgaagg tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac
     2281 accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg
     2341 ccagaccccg atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc
     2401 tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt
     2461 caccggcgat ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag
     2521 gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt
     2581 tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga
     2641 ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga
     2701 gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat
     2761 cggcattgtt ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc
     2821 tggcactgat gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc
     2881 gagcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga
     2941 tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt
     3001 tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc
     3061 gaaccgccag acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc
     3121 gctcctcgcc ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca
     3181 tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac
     3241 gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag
     3301 aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc
     3361 cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac
     3421 gcgccagcgc tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg
     3481 gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca
     3541 gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg
     3601 cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa
     3661 gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg
     3721 ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac
     3781 ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa
     3841 cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa
     3901 cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc
     3961 cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg
     4021 tgatcagtac cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc
     4081 agttgtaaag catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac
     4141 cgtagaagcc ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac
     4201 caataccgat gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa
     4261 gtacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca
     4321 gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat
     4381 aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc
     4441 ggcgggcggt atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc
     4501 agccacgttt ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg
     4561 ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt
     4621 catccactgc aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga
     4681 gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga
     4741 tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat
     4801 ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct
     4861 tcaccagaaa accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca
     4921 gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg
     4981 ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc
     5041 ttttatctaa aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat
     5101 aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa
     5161 gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata
     5221 agattagcct ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc
     5281 agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca
     5341 aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta
     5401 acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc
     5461 acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat
     5521 taaaattcat tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat
     5581 gatttaagca ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa
     5641 ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat
     5701 ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa
     5761 ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc
     5821 tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc
     5881 ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga
     5941 gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc
     6001 tggaagaact gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc
     6061 agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc
     6121 tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca
     6181 gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt
     6241 cacaccagct gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag
     6301 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
     6361 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     6421 ggcagcggga tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     6481 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     6541 taccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     6601 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     6661 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     6721 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     6781 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
     6841 atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt
     6901 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
     6961 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa
     7021 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     7081 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     7141 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     7201 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat
     7261 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     7321 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     7381 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
     7441 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
     7501 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
     7561 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
     7621 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
     7681 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
     7741 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
     7801 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
     7861 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct
     7921 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
     7981 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
     8041 taatgctctc cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact
     8101 ctgatcggtc gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc
     8161 taccagctct cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc
     8221 aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat
     8281 catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc
     8341 tagcgtagcg attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt
     8401 gtacatttat atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa
     8461 cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga
     8521 accctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg
     8581 ccgctggctg cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg
     8641 gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat
     8701 accggtctgg gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc
     8761 ttcagtatga tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa
     8821 ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg
     8881 ctgttgttca cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta
     8941 tttaaccgtt ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg
     9001 ctaccgcata ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg
     9061 tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc
     9121 agctatatgg atggcaacat tcgtaatcta cgctgggtgt ttataatcgg tagtgccatc
     9181 cccctggtgg catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc
     9241 tttatgggat tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa
     9301 atggtggcct ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc
     9361 acgtcatttc tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt
     9421 tcaaataccg ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg
     9481 tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg
     9541 gcggtactgg cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct
     9601 caggcgggtt accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt
     9661 attgctgtga ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga
     9721 tcagatagcc tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct
     9781 cttaatgcag gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac
     9841 gccccggttt ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca
     9901 tccagtatgc atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg
     9961 cttctggcga cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg
    10021 cctccagcgc tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc
    10081 gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca
    10141 agccgaataa cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca
    10201 tatgttgaaa agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca
    10261 catagtcagc cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag
    10321 aactcaacac cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag
    10381 tgttgtattt ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg
    10441 ttttcatacg cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc
    10501 gtatcagaga tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg
    10561 acaagttgca ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt
    10621 gattttattc ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa
    10681 cgccgaggga ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa
    10741 gtggaggcgc gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg
    10801 ctttgttaac gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt
    10861 cttttaaact ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt
    10921 gctctaccaa ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat
    10981 tatcgtctgg caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg
    11041 agtcaacgat atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac
    11101 tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt
    11161 accgcagcca caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac
    11221 agcagccagg ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg
    11281 gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt
    11341 tcacgggcga tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt
    11401 accagcacca tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca
    11461 aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc
    11521 gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc
    11581 aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat
    11641 tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac
    11701 atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt
    11761 ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca
    11821 acatttgccg acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga
    11881 ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat
    11941 gctggataac atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct
    12001 caaagaacag cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca
    12061 gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa
    12121 gctggccttt gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt
    12181 taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc
    12241 ctgtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac
    12301 aggaagcgac ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca
    12361 cttccggcaa tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct
    12421 gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg
    12481 ccctatcgcc gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg
    12541 aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg
    12601 tgcgcatctg gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca
    12661 gttccgtacc gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac
    12721 gaatgaacaa tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa
    12781 ggtcgtcgcc ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt
    12841 ggtcgcgaat acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc
    12901 taatgagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga
    12961 cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc
    13021 ctatctggta ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc
    13081 gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga
    13141 acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt
    13201 gggtatcgcc actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt
    13261 aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat
    13321 gcgtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag
    13381 cgaggttgct gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat
    13441 agataaccgt accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt
    13501 ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca
    13561 cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact
    13621 gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat
    13681 ctcattgacc ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa
    13741 actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc
    13801 gatatcagaa gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc
    13861 ctgcggagcc gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg
    13921 taaagggttt tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc
    13981 gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac
    14041 gtcaacggca tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac
    14101 gacttttata cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg
    14161 gtcatcaaca agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact
    14221 gatagttagt cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt
    14281 gggtattcga tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc
    14341 gtcaccaacg gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat
    14401 tgactttatt cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga
    14461 acaatacggg caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt
    14521 ttatagcact gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat
    14581 ggtatactcc gccgccttaa catttttcac cacaaatttt cattgcaaca tgacgaggaa
    14641 aataaatgag tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag
    14701 tctcctgcct gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg
    14761 gccgcgtgat gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg
    14821 cagtattttc caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg
    14881 gctgagagcg tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct
    14941 tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg
    15001 tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact
    15061 gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa
    15121 aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta
    15181 aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc
    15241 acattaaatg gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat
    15301 tttttgcctg ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag
    15361 gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct
    15421 gagcctgaag ctctatttca tggtaggcct cttctgcggc ctccatcctc tgaaaacgca
    15481 acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc
    15541 ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa gatttatata
    15601 taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat
    15661 taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg
    15721 acaggtgaat cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac
    15781 tggcgggtgc gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact
    15841 atccgcccct ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg
    15901 tgtgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact
    15961 tcgcccacca gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga
    16021 cgcattgcca gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt
    16081 ggatagctgg tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc
    16141 gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc
    16201 accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac
    16261 tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca
    16321 acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt
    16381 ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg
    16441 ataccgtgca gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca
    16501 ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg
    16561 ataacacagt cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct
    16621 ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg
    16681 cggccagcga cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca
    16741 cccgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac
    16801 tggcagcggc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg
    16861 cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt
    16921 cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac
    16981 cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc
    17041 gcatcagcgc gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt
    17101 gcagggtata cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac
    17161 tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc
    17221 gccgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc
    17281 gtcaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag
    17341 acgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc
    17401 aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat
    17461 cgcctgttgt aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac
    17521 gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt
    17581 tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg
    17641 cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac
    17701 cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagtt cttccagttg
    17761 ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa
    17821 caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag
    17881 cgccacgcag tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga
    17941 ctggatcgcg ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag
    18001 aaattccagc ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt
    18061 gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg
    18121 cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc
    18181 cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc
    18241 gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac
    18301 catttttcgg aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc
    18361 accgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag
    18421 aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc
    18481 agacgatcaa cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg
    18541 tcatcatcat aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag
    18601 ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg
    18661 ccttcgttac cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa
    18721 tcgtattttt tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt
    18781 ttagagtcca gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg
    18841 ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca
    18901 tccccctgaa aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgctc
    18961 tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc
    19021 agcgccacgg ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc
    19081 accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca
    19141 actccgctac atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc
    19201 tgcacaagtc cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg
    19261 taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc
    19321 tgcagctgat cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga
    19381 acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta
    19441 aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg
    19501 cacaatgccg cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc
    19561 aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg
    19621 agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg
    19681 cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta
    19741 aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatgtcc
    19801 tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg
    19861 agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt
    19921 tcgatggctt ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt
    19981 tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca
    20041 tcgtaggaga agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata
    20101 tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc
    20161 agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag
    20221 tcacggctgc gaagttcatc cagcatagcg ccacggatac gctacactgc gtaagttgta
    20281 aatgccgttc cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc
    20341 gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct
    20401 tcgtgacgca ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca
    20461 gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta
    20521 tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg
    20581 gttacgcaaa aagccgaatg taaaaaaccc cgccggtggc ggggtttgag aggcattcag
    20641 acgattaacc ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca
    20701 cggagttacc ggcctgctgg ataatctgcg ctttcgacat gttggacact tcggtcgcat
    20761 agtcggcgtc ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt
    20821 tggtgactgc ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga
    20881 tggagctgat ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt
    20941 cagcatctgt cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag
    21001 tcactgaacc atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt
    21061 tgttatcaac ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc
    21121 tataacctgc agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag
    21181 ctgcctgatc aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat
    21241 taccggtaaa ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg
    21301 ctgcaagtgt ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc
    21361 gcccagttgt atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc
    21421 tgatctgaac atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa
    21481 ctcccggggt caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt
    21541 atgacttact tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt
    21601 tattaatggt tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt
    21661 tagcagcatc gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag
    21721 ctaccaagtc agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa
    21781 acccattcag ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc
    21841 cgtcattcgc accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt
    21901 tgaactgggt ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct
    21961 gaatggagtc cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac
    22021 ggatacgctg taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag
    22081 aaataccgtc gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac
    22141 ggttagcaat cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag
    22201 acagacgctc gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga
    22261 tcagcgagag gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag
    22321 tcgttgatta cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt
    22381 ctgtcgtaaa acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga
    22441 aatactcttt tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt
    22501 gcgcaattca ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc
    22561 gcatggcaag tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg
    22621 atagcctcac cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt
    22681 ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg
    22741 ccaatactgc attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca
    22801 ccgcgttcag tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca
    22861 cccatctggc gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg
    22921 cgatcgccac cagcgacagc aaactcacca ttcaacaagg cgacgacaaa gatccgatta
    22981 ccattgatat cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg
    23041 caaaagcggg cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag
    23101 tcacatcaaa tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg
    23161 cgctacaaag ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg
    23221 tcgccgccca gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca
    23281 ctatcagcga cgcgctggaa aacatcactc tgaacctgaa cgatgtcacc acgggcaacc
    23341 agacgctaac catcactcag gacacctcca aagtgcaaac ggcgattaaa gactgggtga
    23401 atgcctataa ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg
    23461 cgggagctga tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga
    23521 cgattcagac gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa
    23581 cgctggcgca gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg
    23641 acaaactcac cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg
    23701 atggtaaaaa aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga
    23761 caacgggcat tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta
    23821 aagactacaa cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat
    23881 ttacccaact ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc
    23941 agttcgaaaa caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg
    24001 cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga aagcaccgta atgagcgcca
    24061 gccaacagca gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc
    24121 gactgtttat gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca
    24181 acatcattga gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc
    24241 aaaacttaat tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca
    24301 acgatgtctc agcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga
    24361 aagagtcttt actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca
    24421 tttgtatttc gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac
    24481 ggaagaacaa tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga
    24541 gatcgcacac ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc
    24601 gatgctgcac ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat
    24661 ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta
    24721 tggcgatcag ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag
    24781 ccgatggctc gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt
    24841 cgaagaatgc gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt
    24901 aagctctggc ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg
    24961 gtctggttgc cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc
    25021 tatgatttat ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt
    25081 gataaagcac aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg
    25141 ctggacgtgg tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag
    25201 cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg
    25261 acgcgttaca ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa
    25321 tgttttagcg gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt
    25381 aacgactata ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat
    25441 tatctgatgg gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac
    25501 tgggcgcgct gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa
    25561 catattccgg catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag
    25621 ccgctgttta ttgtggctga atgctggtcg catgaagtcg ataaactgca aacgtatatt
    25681 gatcaggtgg aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa
    25741 gcatcgcgca tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa
    25801 gccgatcctt ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc
    25861 ctcgaagcac cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg
    25921 gaaaatggcg ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc
    25981 ggtggtgacg ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta
    26041 attctcgccc gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac
    26101 tgcattgcct ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg
    26161 aacggagatg atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg
    26221 cgtgattttc tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc
    26281 ttcttttgca acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat
    26341 ccccggcggc aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc
    26401 cagcgcggcg gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta
    26461 ctcaatggtg ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt
    26521 gagatcatca gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg
    26581 gcaatccaag gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac
    26641 cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt
    26701 tttcacgaca ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc
    26761 tgcaattgcg agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt
    26821 aaagctctga tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt
    26881 ttgtgaatta ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca
    26941 aaccttctga gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg
    27001 attaaattct gggcacccat ccctgcggtc atcgcggcgg gtattctctc cacttactat
    27061 tttggcatta ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag
    27121 ctcctgcagc tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg
    27181 gaaggatcgc cattaacccg gatcgacggc atgatgatcc tcggtatgtt tggcggctgc
    27241 tttgctgctg cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc
    27301 attatgcagg ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc
    27361 tgtaacctgg cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt
    27421 gccatcgcca ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc
    27481 cgtattcccg tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat
    27541 caggcgcggc gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gggctgggcg
    27601 ctgctcacgg cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt
    27661 ggtttactga ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc
    27721 accggacgta cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg
    27781 atcttcagtt acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg
    27841 gtaattggtg gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc
    27901 tggatgtacc gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg
    27961 atcggctcaa cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac
    28021 tgggacaaaa tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat
    28081 ttgctgctgt ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt
    28141 gaggcaccgc agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg
    28201 atatggtggg tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat
    28261 taaaaaaagg tgagatcctg gaagtggtga gcgactgtcc gcagtcgatc aataatattc
    28321 cactggatgc gcgtaatcac ggctacacgg tgctggatat tcagcaagac gggccgacca
    28381 ttcgttattt aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg
    28441 ttacctctga cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag
    28501 gaggttgcta tgtgtggacg ctttgcccaa tcccaaacgc gtgaggatta ccttgcgctt
    28561 ctcgcggaag atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat
    28621 gtcgcgccgg gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat
    28681 ccggttttct ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc
    28741 gtagaaactg cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc
    28801 tgttttgccg atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta
    28861 tctatcgcgc tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc
    28921 gtggtgacga agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag
    28981 atattcatga ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc
    29041 aagacattgg tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc
    29101 agttcacctg gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt
    29161 taattcaacc tgtttgttga catcctccac gccctgaatg acgaggaccc ctgctacgtt
    29221 caggctgtcg cctgaatcat ttcggtgggt tcctgcttca acgagcggcc tgactgcacc
    29281 atctctccac aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc tccatttaca
    29341 tcggcgttcg ctgtatatcc acatacctgg catctgaatt tactttgtga caggcgattt
    29401 tctttcgctg tatgaccaca gcacgcgcaa cgctggcttg tgtacgctgg cggaacagca
    29461 agcacctgac cgccacgcca gagctgcttg tactcaagct ggcggcgcat ttcataccag
    29521 ccctgatcca gtatcgaacg gtttaaacct gattttgccc ggacattgcg ccccggctgg
    29581 ctgaccgtac ccgctgctga ctttgacatg ttgctgacct tcaaatcctc aatgactatc
    29641 attacgtggt ttttgctgac ggtcgttgtg actttgtgaa ggtagtccct gcggatattt
    29701 gcgatacagg aatgcagtcg ctgtat
//
LOCUS       DAJTSF010000043        29466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000043 DAJTSF010000000
VERSION     DAJTSF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.043"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..213)
                     /locus_tag="OO335_002046"
     CDS             complement(<1..213)
                     /locus_tag="OO335_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR6002438.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="OO335_002047"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="OO335_002047"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6002439.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="OO335_002048"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="OO335_002048"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6002440.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="OO335_002049"
     CDS             2894..3634
                     /locus_tag="OO335_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6002441.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="OO335_002050"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="OO335_002050"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6002442.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4192..4794)
                     /locus_tag="OO335_002051"
     CDS             complement(4192..4794)
                     /locus_tag="OO335_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR6002443.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4864..4929
                     /gene="yobI"
                     /locus_tag="OO335_002052"
     CDS             4864..4929
                     /gene="yobI"
                     /locus_tag="OO335_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6002444.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5068..5679
                     /gene="ruvA"
                     /locus_tag="OO335_002053"
     CDS             5068..5679
                     /gene="ruvA"
                     /locus_tag="OO335_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6002445.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5688..6698
                     /gene="ruvB"
                     /locus_tag="OO335_002054"
     CDS             5688..6698
                     /gene="ruvB"
                     /locus_tag="OO335_002054"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6002446.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="OO335_002055"
     CDS             complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="OO335_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6002447.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="OO335_002056"
     CDS             complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="OO335_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6002448.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8461..9393
                     /gene="znuA"
                     /locus_tag="OO335_002057"
     CDS             8461..9393
                     /gene="znuA"
                     /locus_tag="OO335_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6002449.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9409..10731
                     /gene="mepM"
                     /locus_tag="OO335_002058"
     CDS             9409..10731
                     /gene="mepM"
                     /locus_tag="OO335_002058"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6002450.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10851..11822
                     /gene="lpxM"
                     /locus_tag="OO335_002059"
                     /gene_synonym="msbB"
     CDS             10851..11822
                     /gene="lpxM"
                     /locus_tag="OO335_002059"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6002451.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="OO335_002060"
     CDS             complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="OO335_002060"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6002452.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="OO335_002061"
                     /gene_synonym="yebK"
     CDS             complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="OO335_002061"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6002453.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14729..16204
                     /gene="zwf"
                     /locus_tag="OO335_002062"
     CDS             14729..16204
                     /gene="zwf"
                     /locus_tag="OO335_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6002454.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16439..18250
                     /gene="edd"
                     /locus_tag="OO335_002063"
     CDS             16439..18250
                     /gene="edd"
                     /locus_tag="OO335_002063"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6002455.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18287..18928
                     /gene="kdgA"
                     /locus_tag="OO335_002064"
                     /gene_synonym="eda"
     CDS             18287..18928
                     /gene="kdgA"
                     /locus_tag="OO335_002064"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6002456.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18984..20162)
                     /gene="purT"
                     /locus_tag="OO335_002065"
     CDS             complement(18984..20162)
                     /gene="purT"
                     /locus_tag="OO335_002065"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6002457.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20296..20586
                     /gene="yebG"
                     /locus_tag="OO335_002066"
                     /pseudo
     CDS             20296..20586
                     /gene="yebG"
                     /locus_tag="OO335_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20653..21009
                     /locus_tag="OO335_002067"
     CDS             20653..21009
                     /locus_tag="OO335_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6002458.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21336..21995
                     /locus_tag="OO335_002068"
     CDS             21336..21995
                     /locus_tag="OO335_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6002459.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22205..24265
                     /gene="ptrB"
                     /locus_tag="OO335_002069"
     CDS             22205..24265
                     /gene="ptrB"
                     /locus_tag="OO335_002069"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6002460.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="OO335_002070"
     CDS             complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="OO335_002070"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6002461.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24948..25604)
                     /locus_tag="OO335_002071"
                     /pseudo
     CDS             complement(24948..25604)
                     /locus_tag="OO335_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(25706..25936)
                     /gene="holE"
                     /locus_tag="OO335_002072"
     CDS             complement(25706..25936)
                     /gene="holE"
                     /locus_tag="OO335_002072"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6002462.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26075..26449
                     /gene="yobA"
                     /locus_tag="OO335_002073"
     CDS             26075..26449
                     /gene="yobA"
                     /locus_tag="OO335_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6002463.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26453..27325
                     /gene="copD"
                     /locus_tag="OO335_002074"
                     /pseudo
     CDS             26453..27325
                     /gene="copD"
                     /locus_tag="OO335_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            27338..27679
                     /locus_tag="OO335_002075"
     CDS             27338..27679
                     /locus_tag="OO335_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6002464.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28072..29149)
                     /locus_tag="OO335_002076"
                     /pseudo
     CDS             complement(28072..29149)
                     /locus_tag="OO335_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(29115..29396)
                     /locus_tag="OO335_002077"
     CDS             complement(29115..29396)
                     /locus_tag="OO335_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR6002465.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         7436 a         7234 c         7569 g         7227 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc
     4801 attccattga aatagataca aatatacgta atagcataaa aaaattattt ttcaggagtc
     4861 gcaatgtata tttttattac gcatttcttc actgaatatg taatattcaa atatttgctt
     4921 ccaatataac ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg
     4981 caagttttct tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat
     5041 ctttgattca ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa
     5101 aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg
     5161 atgacctgtt tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt
     5221 gtggtgcgtg aagacgcgca actgctgtac ggttttaaca ataaacaaga acgcacgttg
     5281 ttcaaagagt tgatcaaaac caacggcgtc ggcccgaagt tggccctggc gatcctctcc
     5341 ggaatgtcag cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg ggcactggtg
     5401 aaactgccgg gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga
     5461 tttaaaggtt tgcatggcga tctctttacg ccagccgccg acctggtact cacgtctcct
     5521 gctagcccgg cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt ggcgctaggc
     5581 tataaaccac aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga caccagcagt
     5641 gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag
     5701 accgtctgat ttctgccggt accactttgc cggaagatgt agcagatcgc gccattcgcc
     5761 ctaaattact ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca
     5821 tcaaagcagc gaaactgcgc ggcgatgccc tcgatcattt gttgattttt ggtcctccgg
     5881 ggttgggtaa aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca
     5941 cgacttctgg tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc actaaccttg
     6001 aaccgcatga cgtgctgttt attgatgaga tccaccgttt atcgccagtt gttgaagaag
     6061 tgctgtaccc ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg
     6121 cacgctccat taaaattgat ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag
     6181 gttcgctgac atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc
     6241 aggtgccgga tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg cttgagatga
     6301 gtgatgacgg tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt attgccaacc
     6361 gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag
     6421 atatcgctgc tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg
     6481 accgcaaatt gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata
     6541 acctggcggc agccattggc gaagaacgtg aaaccattga agatgtactg gaaccttatt
     6601 tgattcagca aggctttttg cagcgtacac cgcgcgggcg tatggcgacg acgcgggcgt
     6661 ggaatcactt tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg
     6721 catccgacac tggcagtgtg tctgatgcga cactgttcgc gtcttatcag gccagccaga
     6781 gatagcgaac cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga
     6841 tagattagct ggcctgcttt ttcatcatac tgataataaa taacagtgcc gcacatagca
     6901 ccaccgacgg acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg
     6961 ccaccatccc caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac
     7021 gcgcagtagc tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca
     7081 tcgctacacc aatcgtcaat gccgtcacca gcatcaacaa caatttcact tgctgtaatt
     7141 tcacaccatc aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt
     7201 gccagaacaa aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg
     7261 gcgtcactgc cagtaaatca ccgaacaggt aagccatcaa atcaacacga atgttagaca
     7321 tcagactaac gaccactagg cccagcgaca gggcactgtg cgccataatc cctaataacg
     7381 tgtcgatcgc cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg
     7441 taacggcaat caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa
     7501 gtaatgaggc atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg
     7561 aacccagcgg acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata
     7621 attcaatcat gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg
     7681 atgattatga tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga
     7741 aataaactcc ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt
     7801 caggcacagc acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa
     7861 aacgccacaa tccagttcac gacgcaactg gtcaataagg tcatataacg ccacttggcc
     7921 atttacatcc acgccttgag tgggttcatc cagcactaat aattgcgggc aatttaacaa
     7981 tgctcgcgct aatagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt
     8041 aatcaggtgc ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc
     8101 agggcgtaag cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt
     8161 ctgcggtaca tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt
     8221 taccagcccg agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa
     8281 agtcaaaatt tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc
     8341 aaaagaaacc gagacatttt ccagggaaac cagacttgtc atgttaattt tagtcttgca
     8401 gtagtcatga aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt
     8461 atgttacata aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca
     8521 acacaggccg cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct
     8581 gccattgctg atggtgtaac agaaacggag gttttactgc ctgacggcgc ttcagaacat
     8641 gattattcac tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg
     8701 gttggcccgg agatggaagc gtttatgcaa aaaccggtaa gcaaattacc agaagcgaag
     8761 caggtaacga ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc
     8821 gatgatgatg atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc
     8881 aacatgcatc tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga
     8941 aaattagtgg aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt
     9001 gaggcacaat tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg
     9061 aaaggttatt tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca
     9121 ccgcttggtc attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa
     9181 ataagaacac agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg
     9241 ccagcggtcg ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc
     9301 ctggggacga atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc
     9361 aaccagtatg cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata
     9421 gcccgctctg tcgccctggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg
     9481 tcgctcaccg ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgtgat
     9541 gccacgccaa ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct
     9601 gaagccagtg agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa
     9661 ctggatgaca aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat
     9721 acgctaagca gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaacttgct
     9781 gctgccgaca aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc
     9841 gcggacggcg aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat
     9901 gaccgtactg ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt
     9961 aacaatctgc tgaaaggtac cgtcggaggg agctttgttg ccagcgccag aaacgccggt
    10021 ttaaccagcg ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc
    10081 aaactgaaaa aaggcgatga atttgcggta ttaatgtctc gagaaatgct tgatggtaaa
    10141 cgtgagcaaa gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgca
    10201 atccgcgctg aggatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc
    10261 ttgcgattcc cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgaact
    10321 aatccggtga caggtcgcgt tgcaccacac agaggtgttg atttcgccat gccacaaggt
    10381 acgccagtgc tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca
    10441 ggttattatg tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc
    10501 aagattctgg tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt
    10561 aataccggac gttcaactgg gccgcatctg cactatgaag tatggataaa ccagcaggcc
    10621 gtaaacccgc tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc
    10681 gaattcctgg cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc
    10741 attcgcagcc ggtacgcagt cagtaccggc tttttttatt tggtgcgggg caagttgtgc
    10801 cgctacacta tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga
    10861 aaaaaaataa tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact
    10921 ggggagcatg gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc
    10981 gtgatcccat tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc
    11041 gtcgtgcgtt aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag
    11101 cgattgttga tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg
    11161 caatacgcgg gccggagaaa attcagccgc gcgttggctg gcaagggctg gagatcatcg
    11221 aagagatgcg gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg
    11281 atattcctgc catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc
    11341 agggcaaccc ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc
    11401 tgcatgcgag aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg
    11461 gatattattt acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg
    11521 ccacctataa agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc cgtgcgcgcg
    11581 ttgtaccgct gtttccgatt tatgatggca agacgcatcg tctgacgatt caggtgcgcc
    11641 caccgatgga tgatctgtta gaggcggatg atcatacgat tgagcggcgg atgaatgaag
    11701 aagtcgagat ttttgttggt ccgcgaccag aacaatacac ctggatacta aaattgctga
    11761 aaactcgcaa accgggcgaa atccagccgt ataagcgcaa agatctttat cccatcaaat
    11821 aaaaaagcct ctcgcgagga gaggccttcg cctgatgata agttcaagtt tgcttcagaa
    11881 tattcgaaat ctgttgaact atcattgaac tataggccgg atgtggcgtt ttcgccgcat
    11941 ccggcaacgt acttactcta ccgttaaaat acgcgtggta ttagtagaac ccacggtact
    12001 catcacgtcg ccttgggtga caatcaccag gtcaccagac atcaagtaac ctttatcgcg
    12061 cagcagatta accgcttcgc tggcagctgc tacgccgtca ttagcgctat caaagggcac
    12121 cggcgtaacg ccacgataga gagcagtcag gttcagcgta cgttcatggc gcgacatggc
    12181 gaaaattggt agaccagagc tgatacggga ggtcatcagc gcggtacgac ccgattcggt
    12241 catggtaatg atcgccgtaa cgcccttcag gtggttagct gcgtacattg ccgacatggc
    12301 aatagcttct tccacattgt cgaactgaac gtccagacgg tgtttagaaa cgttgatgct
    12361 cgggattttt tccgcaccca ggcaaacgcg cgccatggct gcaacagttt ctgacggata
    12421 ctgcccagcg gcagtttctg cagacagcat cacagcgtca gtaccatcca gaacggcgtt
    12481 tgctacgtcc atgacttctg cacgcgtcgg catcgggtta gtaatcattg actccatcat
    12541 ctgggtcgcc gtgattaccg ctcggtttag ctgacgcgca cgacggatca acgctttctg
    12601 aatgccgacc agttccgggt cgccaatttc cacaccgagg tcgccacgtg caaccattac
    12661 cacgtcagag gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg cttccgcacg
    12721 ttcaaccttg gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc gacgggcata
    12781 gttcagatct tcgccacagc gtgggaagga gacagccagg taatctacgc caatcaacgc
    12841 cgcagtctta atgtctgctt tgtctttttc ggtcagcgct tcagccgaca aaccgccgcc
    12901 aagtttgttg atacctttat tgttggagag gggaccaccg acggtgactt cggtgaacac
    12961 tttcatgccc tgaacttcca gtacttttaa ctggacgcga ccatcgtcca gcagcaggat
    13021 gtcaccaggc acgacgtcag taggcaggcc tttgtagtcg ataccgactt tttctttgtc
    13081 gccttcacct ttacccaggt tggcgtcgag caggaattta tccccaatat tgaggaaaac
    13141 tttgccttct ttaaaggtgg atacacggat tttgggcccc tggaggtcac ccagaatagc
    13201 cacatgacgc cccagttttg cggcaatctc acgaacttta tccgcgcgca ttttgtgatc
    13261 ttcaggcgag ccgtgagaaa agttcatacg tacaacgttg gcacccgccg cgataacttt
    13321 ttcaagatta ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt ttgttctgcg
    13381 aagccttttg gacatgtaat actccgttga ctgaaacaac caggtgttgc ttgaacatga
    13441 aatccgaatg aaataacgcc gcgatggcga taaattaatc tcattcggat gtcgtataag
    13501 attaggacag tgacagtcgc ttttagcgat cgtcacttaa attaagtaac tgcttatcaa
    13561 aacgcgattc cttcagcgct tccttgaccc gcttcaagtt atctctgaat tttgcaccgc
    13621 gtcgcaaagt aaatcctgtc gccagcacat ctatcacggt cagctgtgca agtcgagaaa
    13681 ccatgggcat ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc agcgttgctt
    13741 cccgggcgag cggggtaccc gcagaggtga gggcaatcac catggcgtcg ttttcgcgtg
    13801 ccagctgcgc cagctcgacc agattttttg ttcttcctgt gtgagaaatc agcaccacca
    13861 cgtctccgtc gctacaattc atacaactca tgcgttgcag cacgatatca tcggagtaca
    13921 ccaccggaac attaaaacga aagaacttat tcatcgcatc gtgggcaacg gcggcagaag
    13981 agcctaatcc gaaaaaggcg attttttttg cctgagtgag caagtcgacg gcgcggttga
    14041 tggcagattt atccagtgaa tgacggacat gatcgagcgt tgccattgcg gactcaaata
    14101 ttttccccgt gtatgattca acgctgtcat cttcattgac gttgcgatta acatagggag
    14161 tgccattcgc cagactttgt gccagatgaa gtttaaaatc aggaaaaccg cgcgtgtcca
    14221 tgctgcgaca gaaacgattc accgtcggtt cgctaacatt ggcttccagc gccagagcag
    14281 caatactcga atggatcgcg ttatcgggcg aagccagaat gacctcggca actttgcgct
    14341 ctgatttgct caaatgttcc agctgagact ggattttttc cagcatattc atgatgtaaa
    14401 gagactcacg ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag ttttgtcaga
    14461 tattacgcct gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt atttttttca
    14521 taacatgatc agtgtcagat ttttaccgaa tggaaaacga tgattttttt atcagttttg
    14581 ccgcgctttg cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac agttttcgca
    14641 agctcgtaaa agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta
    14701 ccgggttagt taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga
    14761 cctggtcatt ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta
    14821 tcaactggaa aaagtcggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc
    14881 tgactgggat aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa
    14941 agaaaccatt gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct
    15001 cgatgtcaat gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg
    15061 tatcactatt aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct
    15121 tggcgaggca aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac
    15181 gtcgctggcg acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca
    15241 ggtttaccgt atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg
    15301 ttttgctaac tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat
    15361 taccgtggca gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca
    15421 gatgcgtgac atgattcaga accacctgct acaaattctc tgcatgattg cgatgtctcc
    15481 gccatctgac ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtccct
    15541 gcgccgcatc gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg
    15601 cttcgcccag ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaaaagcag
    15661 caatacagaa accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt
    15721 gccattctac ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta
    15781 tttcaaaaca cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa
    15841 actgactatc cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc
    15901 tggccttgac cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac
    15961 ctttaatcag acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg
    16021 tattcaggca ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc
    16081 cattactgag gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg
    16141 gggacccgtt gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga
    16201 gtaatatcag cgcttatcct ttatggttat tttaccggta acatgatctt gcacagattg
    16261 tagaacaatt tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta
    16321 cactgactga aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc
    16381 tctgaatgaa acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat
    16441 gaatccacaa ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc
    16501 tgcttatctc gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc
    16561 atgcggtaac ctggcacacg gtttcgctgc ctgccagcca gaaaacaaag cctctttgaa
    16621 aagcatgttg cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgcgca
    16681 ccagccttat gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt
    16741 tggtcaggtt gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg
    16801 aatggaattg tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tggggctgtc
    16861 ccataacatg tttgatggtg ctctgttcct cggtgtgtgc gacaagattg tcccgggtct
    16921 gacgatggca gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat
    16981 ggcaagcggt ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa
    17041 agtggaccgc atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg
    17101 tactttctac ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt
    17161 gccaggctct tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc
    17221 tgcgcgtcag gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat
    17281 gatcgatgag aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac
    17341 taaccacacc atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga
    17401 tgacttctct gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc
    17461 ggccgatatt aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct
    17521 caaagcaggc ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta
    17581 tacccttgaa ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc
    17641 actcgacagc aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa
    17701 agtgttaagc ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa
    17761 ccaggtgatt gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt
    17821 tgaagcgggt ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc
    17881 gaacggaatg ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg
    17941 tttcaaaatt gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc
    18001 agctatccac gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg
    18061 ggacatcatt cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga
    18121 actggctgct cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg
    18181 tgaattattc agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat
    18241 cactttttaa gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa
    18301 aacaagtgca gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa
    18361 actggaacac gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga
    18421 agtgactctg cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc
    18481 tgaagcgatt gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga
    18541 agcgggtgca cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac
    18601 cgaagggact attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat
    18661 ggactacggt ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc
    18721 cctgcaggcg atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc
    18781 tccggctaac taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg
    18841 gctggttccg gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg
    18901 tgaagctgta gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt
    18961 tttgtagcgt ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc
    19021 gcgcgttcaa tggcgtcaac aacactctct gaagtagcca gtaccacccc cagatgacgg
    19081 ctgccatcaa tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc
    19141 tgcacattat caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct
    19201 gcaggaccat actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg
    19261 gcaaactctg agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg
    19321 gagacctcac tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca
    19381 taaccgccca gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc
    19441 gggctcattt gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct
    19501 actggtgcac agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag
    19561 tcaaacttaa cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga
    19621 gcgtattccc atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg
    19681 gaagagctca tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg
    19741 aaaaggcttt tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc
    19801 gccaggcgac ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca
    19861 acattcagtc cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc
    19921 ggcacgatat aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc
    19981 atattaatga catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg
    20041 acggcaatca cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca
    20101 ccggagccta ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc
    20161 ataacgaata tcccctgatt aattccgcgc gcagtataaa cggaaacgtt tgcgtgtgtc
    20221 tttattgctg atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat
    20281 tatgagaggc tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag
    20341 aaaatgtcgt ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg
    20401 gatctgcttg acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa
    20461 gccctttcgc tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc
    20521 aaattaccgt ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg
    20581 gcctgaagca acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct
    20641 atggagaaaa acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc
    20701 gcatcagttt tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa
    20761 ggtctggatg ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg
    20821 gcgcgctggg cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt
    20881 ttgcaggaca ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa
    20941 agtgccgata ttcattacca ggtctgcgtg gactgcaaag cgggaatggc ggaatatcag
    21001 cggcgttaat ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg
    21061 tccgtcccag aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc
    21121 aatcagcatc aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat
    21181 cacctgatat acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc
    21241 cccgcttatt tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg
    21301 ctacaggcac taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc
    21361 aatccctgct tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca
    21421 aactgttagt gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag
    21481 cacgtaaact tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag
    21541 cgggtacggt gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac
    21601 cgcagtttgg cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc
    21661 tggtctttgc cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc
    21721 agcaattgcg tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg
    21781 aacaaccgct ggatccacaa cgcttggcta ccggggtccg caatgaagaa gaggcgctgg
    21841 aaatctattt cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc
    21901 tgaatgcact gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg
    21961 atctcgaaca gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt
    22021 tgactatttc gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct
    22081 gcataacatt cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt
    22141 cgcttgcatc cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa
    22201 taacatgcta ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg
    22261 catcgataat tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta
    22321 cctgcaacaa gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga
    22381 tcgcatctta aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat
    22441 aaaaaatggc taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca
    22501 gcgtcaatcg gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa
    22561 gcgcgcagct catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac
    22621 cattatggcg ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa
    22681 tctggaaaca ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg
    22741 ggcaaatgac tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta
    22801 tcaggtctgg cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga
    22861 aaaagacgat acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat
    22921 tcatctggcc agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc
    22981 cgagccgttt gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca
    23041 gcatcgtttt tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg
    23101 tatgcgtgat gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga
    23161 agggtttacg ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag
    23221 tttgcgccaa attaaccgca agacccggga agtcattggc attgcctttg atgatccggc
    23281 ctatgtgacc tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta
    23341 ttcttccatg accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg
    23401 tgtattaaaa caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct
    23461 gtggatagtc gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca
    23521 ttttcgcaaa ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat
    23581 tgatgccgat ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat
    23641 tgtccatgtt cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct
    23701 gaagaagaaa aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg
    23761 ctatggctct ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg
    23821 cgttgcaatt aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt
    23881 tgatgttgta acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga
    23941 gtggggaaat ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga
    24001 caacgtcacc gcacaggctt atccgcattt actggtaacg accggtttac acgattctca
    24061 ggtgcaatat tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga
    24121 ccatctttta ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt
    24181 taaatcgtac gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac
    24241 attacccgct acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag
    24301 ctccgggctc atgctgtcca ggttattaaa taaccagcgc agatagcccg gatagcgttc
    24361 ggcaacgtcg gaaaccgctt tgccacggta tttgccaaag gtgaaggttg tcatcaacga
    24421 cggacgtccg gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc
    24481 gataagcaac gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccag
    24541 cggtgtctgt acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc
    24601 tggccacaaa cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag
    24661 tacgcggcgg tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg
    24721 gatcacatct tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc
    24781 catcgcttgt ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat
    24841 ttttccgtca atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt
    24901 ttctgtatcg ataatgcgca acatggcatg atccaggccg aaaaagccta gcgtaatgga
    24961 atgatatcgc cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga
    25021 tcggcgcgaa cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt
    25081 gccatcagca cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc
    25141 agcaggtcgg gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata
    25201 tccatacctt gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat
    25261 gcaccatgac tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt
    25321 ccacgaataa agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag
    25381 gtcgttgcgg cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca
    25441 cgtcgcgaat aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt
    25501 tgcctggcgg cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt
    25561 ttacgaggct catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca
    25621 aaatgactgc aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt
    25681 aagaaagtgt aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca
    25741 gattgaccga agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt
    25801 ctggctgttc acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag
    25861 ccaccccggc cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag
    25921 ccagattctt cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga
    25981 gcctggatgg caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc
    26041 tgtaaaaaag ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg
    26101 ctacgcgctg gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca
    26161 tttaacgcat cagtatcctg cggcaaacgc gcaagtgaca gctgccccgc aggcaatcac
    26221 tttaaacttc tcggaaggcg ttgaaacagg attcagtggt gcaaaaatca cggggccaaa
    26281 aaacgaaaat attaaaacat taccagcgaa gcggaatgag caggaccaaa agcaactgat
    26341 tgttccactg gccgattcgc tgaaacccgg aacctacacc gttgactggc atgttgtttc
    26401 ggtggacggg cataaaacga aaggacacta cacctttagc gtgaaataaa tcatgctggc
    26461 gtttacctgg atcgcgctgc gatttatcca tttcacctcg ttgatgctgg tctttggctt
    26521 tgcaatgtat ggtgcctggc tggccccctt aatgattcgt cgcttgctga cgaagcgttc
    26581 tctgcgttta caacagcacg ctgccgtctg gagtttgatc agtgccactg caatgctcgc
    26641 cgttcagggc gggttgatgg gaacgggatg gacagatgta ttttcgccaa acatttggca
    26701 ggctgttttg caaacgcagt ttggtggcgt ctggttatgg caaattgttc tcgccctcgt
    26761 aacgttgatc gtcgccctta tgcaaccgcg gaatatgcca cgtttgctgt ttatgctcac
    26821 taccgcgcaa tttattctgc tggcgggagt ggggtatgcg acgctgaatg aaggggtaac
    26881 agcgaaaatc catcagacta atcacgcgat tcacctgatt tgtgcggcgg cgtggtttgg
    26941 gggattactg ccggtgctct ggtgtatgca actcatcaaa ggccgttggc gacactaggc
    27001 tattcaggcg ctgatgcgtt tttcctggtg cgggcatttt gcggtgatcg gcgtactggc
    27061 aagcggcgtg cttaatgcat tgttgattac cggatttccc cccacgctta cgacttactg
    27121 gggccaactc ttattgctca aagccatcct ggtgatgatt atggtggtca ttgcgctggc
    27181 taatcgttat gttcttgtac cgcgcatgag gcaggatgaa gatcgtgcgg caccctggtt
    27241 tgtgtggatg actaaactcg aatgggcgat aggtgcggta gtgctggtga tcatcagcct
    27301 gcttgcgact ctcgaacctt tttgatggat tacgaaaatg atgaaaaaaa gtattctggc
    27361 gtttctgtta ctcaccagtt ctgcagcggc gctggcggct ccacaggtga taaccgtgag
    27421 tcgttttgaa gtgggtaaag acaaatgggc gtttaatcgc gaagaggtaa tgctgacttg
    27481 ccgaccgggt aatgctttgt atgtcatcaa cccaagtacc ctcgtgcagt atcctttaaa
    27541 cgatatcgca caaaaggaag ttgccagtgg gaagaccaat gcccaaccca tttcggtgat
    27601 tcagattgat gatcctaaca atcccggcga aaaaatgagt ctggcaccgt ttatagaacg
    27661 agctgaaaaa ctctgttaat tacctaaaat agccttttga tttccaataa aaaaaccgcc
    27721 tcagttcttt caccagaacg ggcggttttt aacatttaag ctgatgagca ccgcgctttt
    27781 tattgaccat tttgcacgca aactggaaaa cctggcgtcg tcatctattc ttaaagggca
    27841 aggcaactaa gcctgcatta atgccaactt ttagcgcacg gctctctccc aagagccatt
    27901 tccctggacc gaatacagga atcgtattcg gtcttttttt aattgtattt aaaatcaatt
    27961 agttgcaaac gtctccccga aattccccga aatttactcg aatttctgta ttccggtctt
    28021 ttttggttat atcacaacca aaatacattt aacaatccat ttacgttaaa atcagagcag
    28081 taagtacgtt ttttctctct catcaagata catttttgtt gtcttctccg atgtgtggcc
    28141 aagtagacgc tgagcaaatt cttctccaca tatttctttg tacaatcgtc cagccaggct
    28201 tctgatttcg tgaaaagttg gtgggttttc actgaacagt gatacctgtt aattttctgg
    28261 ctgcgacaaa tttctttgtc aggccgtccg ggtgaatgct gccgtcaggg ctgttttttc
    28321 taatcccggc actgattaga taatcccccc tacttaccat gcggcactgt tcaactactg
    28381 caccaagtcg taaaccagcg acaggaaggc tgagtgacag ggggatagca atcatcattc
    28441 ctgtcttaat ttgcctgatg tggagacgat catcataaat atcactgaac cgcatattcg
    28501 ttatgtcttc gcgacgttgt cctgttacaa gggctaagtc catagctaat gggaaccatg
    28561 ccggaagttg atctgctgcc tccctgatgc agttgtatgt ctttagtttc agccgttctc
    28621 ttgtaactac tattttcggt gctcttgttg gcgttactgg attttgagat atacgtcctt
    28681 caacaatggc ttcgcggaac atatcagata acacagaacg cattgatcct gccatcgtgt
    28741 ttttccctcc ttcaatctac aaatcaagaa actcggcgat atggcgagtg gttatttctg
    28801 tcagtaaaac ccctcctaat ttttctttta ttgtctccag ttgatttacc cgagttttat
    28861 aagtatttct ggacgctttt ctccttataa gaatcgtttt gtaacgttca atccagcctg
    28921 ccatagtaaa tgagtcgacc cctttaagct tttcaattag ggcagcagga gagtagtttt
    28981 tgtatatata atgatttgct tcaattgcct gcgctactgc atctcttctt gaaattttac
    29041 ctagtgtaaa ttcttctttc gtcagagggt tgcgccagta atatgctttg tctctccttc
    29101 gatatgttaa gtttctaggc aaattgggat cgtatttttt ccgctgcatg ttttaacttc
    29161 tccattaacg gactgtctct cccttgtcgc ccattaggct gatggtgtgt tatatcggta
    29221 tcaaccttat ttgggttgat atagaaagcc tccggaacca ccctgtaact cctcccgtgt
    29281 agttcaggtg caggataaat gtttccattc cttgcccatc gtctcagcgt tgatattgat
    29341 ggtgggttat ccggatatct gagttttccc cacgttttga gtgtcacaag attcattgcc
    29401 atacctctca cgatatgacc gccagtaaat atacagaata ctggcgggtg tggttgattt
    29461 ttaata
//
LOCUS       DAJTSF010000044        29351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000044 DAJTSF010000000
VERSION     DAJTSF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.044"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="OO335_002078"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="OO335_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6002466.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="OO335_002079"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="OO335_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR6002467.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO335_002080"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO335_002080"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6002468.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="OO335_002081"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="OO335_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6002469.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="OO335_002082"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="OO335_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6002470.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="OO335_002083"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="OO335_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6002471.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="OO335_002084"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="OO335_002084"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6002472.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="OO335_002085"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="OO335_002085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6002473.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="OO335_002086"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="OO335_002086"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6002474.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFINGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="OO335_002087"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="OO335_002087"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6002475.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="OO335_002088"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="OO335_002088"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6002476.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="OO335_002089"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="OO335_002089"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6002477.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="OO335_002090"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="OO335_002090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6002478.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="OO335_002091"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="OO335_002091"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6002479.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="OO335_002092"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="OO335_002092"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6002480.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="OO335_002093"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="OO335_002093"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6002481.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="OO335_002094"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="OO335_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6002482.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="OO335_002095"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="OO335_002095"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6002483.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19508..20329
                     /locus_tag="OO335_002096"
     CDS             19508..20329
                     /locus_tag="OO335_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR6002484.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20316..22424
                     /locus_tag="OO335_002097"
     CDS             20316..22424
                     /locus_tag="OO335_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6002485.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22421..24088
                     /locus_tag="OO335_002098"
     CDS             22421..24088
                     /locus_tag="OO335_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6002486.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24091..25617
                     /locus_tag="OO335_002099"
                     /pseudo
     CDS             24091..25617
                     /locus_tag="OO335_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
     gene            25618..27234
                     /locus_tag="OO335_002100"
     CDS             25618..27234
                     /locus_tag="OO335_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6002487.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27290..27442)
                     /locus_tag="OO335_002101"
                     /pseudo
     CDS             complement(27290..27442)
                     /locus_tag="OO335_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27465..27842)
                     /locus_tag="OO335_002102"
     CDS             complement(27465..27842)
                     /locus_tag="OO335_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002488.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(28252..28623)
                     /locus_tag="OO335_002103"
     CDS             complement(28252..28623)
                     /locus_tag="OO335_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002489.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(28684..29181)
                     /locus_tag="OO335_002104"
     CDS             complement(28684..29181)
                     /locus_tag="OO335_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002490.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(29210..29351)
                     /locus_tag="OO335_002105"
                     /pseudo
     CDS             complement(29210..29351)
                     /locus_tag="OO335_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase"
BASE COUNT         7575 a         6854 c         7551 g         7371 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagta gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt attaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggct ggcgttatca tccgatcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggag gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagat aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctag cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgctgtgggc
    19381 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac
    19441 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac
    19501 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa
    19561 agaggggcgt ggccaagggc atggtaaaga ctatattcca tggctaacag tacaagaagt
    19621 tccttcttca ggtcgttccc accgtattta ttctcataag acgggacgag tccatcattt
    19681 gctatctgac ttagagcttg ctgtttttct cagtcttgag tgggagagca gcgtgctaga
    19741 tatacgcgag cagttcccct tattacctag tgataccagg cagattgcaa tagatagtgg
    19801 tattaagcat cctgttattc gtggtgtaga tcaggttatg tctactgatt ttttagtgga
    19861 ctgcaaagat ggtccttttg agcagtttgc tattcaagtc aaacctgcag cagccttaca
    19921 agacgagcgt accttagaaa aactagaact agagcgtcgc tattggcagc aaaagcaaat
    19981 tccttggttc atttttactg ataaagaaat aaatcccgta gtaaaagaaa atattgaatg
    20041 gctttattca gtgaaaacag aagaagtttc tgcggagctt ttagcacaac tatccccatt
    20101 ggcccatatc ctgcaagaaa aaggagatga aaacattatc aatgtctgta agcaggttga
    20161 tattgcttat gatttggagt taggcaaaac attgagtgag atacgagcct taaccgcaaa
    20221 tggttttatt aagttcaata tttataagtc tttcagggca aataagtgtg cagatctctg
    20281 tattagccaa gtagtgaata tggaggagtt gcgctatgtg gcaaattaat gaggttgtgc
    20341 tatttgataa tgatccgtat cgcattttgg ctatagagga tggccaagtt gtctggatgc
    20401 aaataagcgc tgataaagga gttccacaag ctagggctga gttgttgcta atgcagtatt
    20461 tagatgaagg ccgcttagtt agaactgatg acccttatgt acatcttgat ttagaagagc
    20521 cgtctgtaga ttctgtcagc ttccagaagc gcgaggagga ttatcgaaaa attcttccta
    20581 ttattaatag taaggatcgt ttcgacccta aagtcagaag cgaactcgtt gagcatgtgg
    20641 tccaagaaca taaggttact aaggctacag tttataagtt gttacgccgt tactggcagc
    20701 gtggtcaaac gcctaatgca ttaattcctg actacaaaaa cagcggtgca ccaggggaaa
    20761 gacgttcagc gacaggaaca gcaaagattg gccgagccag agaatatggt aagggtgaag
    20821 gaaccaaggt aacgcccgag attgaacgcc tttttaggtt gaccatagaa aagcacctgt
    20881 taaatcaaaa aggtacaaag accaccgttg cctatagacg atttgtggac ttgtttgctc
    20941 agtattttcc tcgcattccc caagaggatt acccaacact acgtcagttt cgttattttt
    21001 atgatcgaga ataccctaaa gctcagcgct taaagtctag agttaaagca ggggtatata
    21061 aaaaagacgt acgaccctta agtagtacag ccacttctca ggcgttaggc cctgggagtc
    21121 gttatgagat tgatgccacg attgctgata tttatttagt ggatcatcat gatcgccaaa
    21181 aaatcatagg aagaccaacg ctttacattg tgattgatgt gtttagtcgg atgatcacgg
    21241 gcttttatat cggctttgaa aatccgtctt atgtggtggc gatgcaggct tttgtaaatg
    21301 cttgctctga caaaacggcc atttgtgccc agcatgatat tgagattagt agctcagact
    21361 ggccgtgtgt aggtttgcca gatgtgttgc tagcggaccg tggcgaatta atgagtcatc
    21421 aggtcgaagc cttagtttct agttttaatg tgcgagtgga aagtgctcca cctagacgtg
    21481 gcgatgctaa aggcatagtg gaaagcactt ttagaacact acaagccgag tttaagtcct
    21541 ttgcacctgg cattgtagag ggcagtcgga tcaaaagcca tggtgaaaca gactataggt
    21601 tagatgcatc tctgtcggta tttgagttca cacaaattat tttgcgtacg atcttattca
    21661 gaaataacca tctggtgatg gataaatacg atcgagatgc tgattttcct acagatttac
    21721 cgtctattcc tgtccagcta tggcaatggg gtatgcagca tcgtacaggt agtttaaggg
    21781 ctgtggagca agagcagttg cgagtagcgt tactgcctcg ccgaaaggtc tctatttctt
    21841 catttggcgt taatttgtgg ggtttgtatt actcggggtc agagattctg cgtgagggtt
    21901 ggttgcagcg gagcactgat atagctagac ctcaacattt agaagcggct tatgacccag
    21961 tgctggttga tacgatttat ttgtttccgc aagttggcag ccgtgtattt tggcgctgta
    22021 atctgacgga acgtagtcgg cagtttaaag gtctctcatt ttgggaggtt tgggatatac
    22081 aagcacaaga aaaacacaat aaagccaatg cgaagcagga tgagttaact aaacgcaggg
    22141 agcttgaggc gtttattcag caaaccattc agaaagcgaa taagttaacg cccagtacta
    22201 ctgagcccaa atcaacacgc attaagcaga ttaaaactaa taaaaaagaa gccgtgacct
    22261 cggagcgtaa aaaacgtgcg gagcatttga agccaagctc ttcaggtgat gaggctaaag
    22321 ttattccttt caacgcagtg gaagcggatg atcaagaaga ttacagccta cccacatacg
    22381 tgcctgaatt atttcaggat ccaccagaaa aggatgagtc atgagtgcta cccggattca
    22441 agcagtttat cgtgatacgg gggtagaggc ttatcgtgat aatcctttta tcgaggcctt
    22501 accaccatta caagagtcag tgaatagtgc tgcatcactg aaatcctctt tacagcttac
    22561 ttcctctgac ttgcaaaagt cccgtgttat cagagctcat accatttgtc gtattccaga
    22621 tgactatttt cagccattag gtacgcattt gctactaagt gagcgtattt cggtcatgat
    22681 tcgaggtggc tacgtaggca gaaatcctaa aacaggagat ttacaaaagc atttacaaaa
    22741 tggttatgag cgtgttcaaa cgggagagtt ggagacattt cgctttgagg aggcacgatc
    22801 tacggcacaa agcttattgt taattggttg ttctggtagt gggaagacga cctctcttca
    22861 tcgtattcta gccacgtatc ctcaggtgat ttaccatcgt gaactcaatg tagagcaggt
    22921 ggtgtatttg aaaatagact gctcgcataa tggttcgcta aaagaaatct gcttgaattt
    22981 tttcagagcg ttggatcgag ccttgggctc gaactatgag cgtcgttatg gcttaaaacg
    23041 tcatggtata gaaaccatgt tggctttgat gtcgcaaata gccaatgcac atgctttagg
    23101 gttgttggtt attgatgaaa ttcagcattt aagccgctct cgttcgggtg gatctcaaga
    23161 gatgctgaac ttttttgtga cgatggtgaa tattattggc gtaccagtga tgttgattgg
    23221 tacccctaaa gcacgagaga tttttgaggc tgatttgcgg tctgcacgta gaggggcagg
    23281 gtttggagct atattctggg atcctataca acaaacgcaa cgtggaaagc ccaatcaaga
    23341 gtggatcgct tttacggata atctttggca attacagctt ttacaacgca aagatgcgct
    23401 gttatcggat gaggtccgtg atgtgtggta tgagctaagc caaggagtga tggacattgt
    23461 agtaaaactt tttgtactcg ctcagctccg tgcgctagct ttaggcaatg agcgtattac
    23521 cgctggttta ttgcggcaag tgtatcaaga tgagttaaag cctgtgcacc ccatgctaga
    23581 ggcattacgc tcgggtatcc cagaacgcat tgctcgttat tctgatctag tcgttcccga
    23641 gattgataaa cggttaatcc aacttcagct agatatcgca gcgatacaag aacaaacacc
    23701 agaagaaaaa gcccttcaag agttagatac cgaagatcag cgtcatttat atctgatgct
    23761 gaaagaggat tacgattcaa gcctgttaat tcccactatt aaaaaagcgt ttagccagaa
    23821 tccaacgatg acaagacaaa agttactgcc tcttgttttg cagtggttga tggaaggcga
    23881 aacggtagtg tcagaactag aaaagccctc caagagtaaa aaggtttcgg ctataaaggt
    23941 agtcaagccc agcgactggg atagcttgcc tgatacggat ttacgttata tctattcaca
    24001 acgccaacct gaaaaaacca tgcatgaacg gttaaaaggg aaaggggtaa tagtggatat
    24061 ggcgagctta tttaaacaag caggttagcc atgagaaact ttcctgttcc gtactcgaat
    24121 gagctgattt atagcactat tgcacgggca ggcgtttatc aagggattgt tagtcctaag
    24181 cagctgttgg atgaggtgta tggcaaccgc aaggtggtcg ctaccttagg tctgccctcg
    24241 catttaggtg tgatagcaag acatctacat caaacaggac gttacgctgt tcagcagctt
    24301 atttatgagc ataccttatt ccctttatat gctccgtttg taggcaagga gcgccgagac
    24361 gaagctattc ggttaatgga gtaccaagcg caaggtgcgg tgcatttaat gctaggagtc
    24421 gctgcttcta gagttaagag cgataaccgc tttagatact gccctgattg cgttgctctt
    24481 cagctaaata ggtatgggga agccttttgg caacgagatt ggtatttgcc cgctttgcca
    24541 tattgtccaa aacacggtgc tttagtcttc tttgatagag ctgtagatga tcactgacat
    24601 caattttggg ctttgggtca tactgagctg ctttcagact accccaaaga ctccctatct
    24661 caattaacag cactagctgc ttatatagcc cctctgttag atgctccacg agcgcaagag
    24721 ctttccccaa gccttgagca gtggacgctg ttttatcagc gcttagcgca ggatctaggg
    24781 ctaaccaaaa gcaagcacat tcgtcatgac ttggtggcgg agagagtgag gcagactttt
    24841 agtgatgagg cactagagaa actggattta aagttggcag agaacaagga cacgtgttgg
    24901 ctgaaaagta tattccgtaa gcatagaaaa gcctttagtt atttacagca tagtattgtg
    24961 tggcaagcct tattgccaaa actaacggtt atagaagcgc tacagcaggc aagtgctctt
    25021 actgagcact ctataacgac aagacctgtt agccagtctg tgcaacctaa ctctgaagat
    25081 ttatctgtta agcataaaga ctggcagcaa ctagtgcata aataccaagg aattaaggcg
    25141 gcaagacagt ctttagaggg tggggtgcta tacgcttggc tttaccgaca tgacagggat
    25201 tggctagttc actggaatca acagcatcaa caagagcgtc tggcacccgc ccctagagtt
    25261 gattggaacc aaagagatcg aattgctgta cgacaactat taagaatcat aaagcgtcta
    25321 gatagtagcc ttgatcaccc aagagcgaca tcgagctggc tgttaaagca aactcctaac
    25381 ggaacctctc ttgcaaaaaa tctacagaaa ctgcctttgg tagcgctttg cttaaagcgt
    25441 tactcagaga gtgtggaaga ttatcaaatt agacggatta gccaagcttt tattaagctt
    25501 aaacaggaag atgttgagct taggcgctgg cgattattaa gaagtgcaac gttatctaaa
    25561 gagcggataa ctgaggaagc acaaagattc ttggaaatgg tttatgggga agagtgagtg
    25621 gttaggctag ctacatttaa tgacaatgtg caggttgtac atattggtca tttattccgt
    25681 aactcgggtc ataaggagtg gcgtattttt gtttggttta atccaatgca agaacggaaa
    25741 tggactcgat ttactcattt gcctttatta agtcgagcta aggtggttaa cagtacaaca
    25801 aagcaaataa ataaggcgga tcgtgtgatt gagtttgaag catcggatct tcaacgagcc
    25861 aaaataatcg attttcctaa tctctcgtcc tttgcttccg tacgcaacaa ggatggagcg
    25921 cagagttcat ttatttacga agctgaaaca ccatatagca agactcgtta tcacatccca
    25981 cagttagagc tagctcggtc attattttta attaactcct atttctgtcg aagctgtttg
    26041 agcagtaccg ctttacagca agagttcgac gttcagtatg aggttgagcg agatcattta
    26101 gagataagga tcttacccag ttcatcgttt cctaaagggg cgttagagct gtcggccgta
    26161 gtgcagcttt tggtttggtt gttttcggat caagatgtta tggattcgta tgaaagtatt
    26221 tttaggcact atcaacaaaa tagagaaatt aagaacggcg ttgaaagctg gtgctttagc
    26281 tttgaccctc cgcccatgca gggttggaaa ttacatgtaa aaggacgttc ttctaacgag
    26341 gataaggatt atttagttga ggaaatagta ggtttagaaa tcaacgctat gcttcctagc
    26401 acaacagcta ttagccatgc ctcttttcag gaaaaggagg caggtgatgg tagtacgcag
    26461 cacatagcgg tttcaacaga gtcagttgtt gatgatgagc atctacagtt ggacgatgag
    26521 gaaacagcca atatagacac agacacacga gtcatagagg ctgagccgac atggataagt
    26581 tttagtagac ctagtcgaat tgaaaaatct cgcagggcaa gaaaaagtag ccaaactatt
    26641 ttagaaaaag aagaagcaac aacaagtgaa aatagtaatt tggttagtac tgatgagcca
    26701 cacttaggtg gtgtcctagc agcggcagat gtgggtggga agcaggatgc aaccaattac
    26761 aactctattt ttgctaatcg atttgctgct tttgatgagc tactttcaat tctaaaaact
    26821 aaatttgcat gtcgggtgct ttttgaagaa accttggttt tgccaaaagt tgggcgtagc
    26881 cgattacatc tgtgtaaaga tggctcacca agagtgatta aagccgttgg ggtgcaacgt
    26941 aatggcagtg aatttgtatt gctagaggtg gatgcatcgg atggggtgaa aatgctttct
    27001 accaaagtgt tgagtggcgt tgatagcgaa acatggcgga atgattttga aaagatacgg
    27061 cgtggagtgg tgaagagctc attgaattgg ccaaatagtt tgtttgatca attatatgga
    27121 caagacgggc atagaggggt gaatcatcca aaggggttgg gggagctgca agtatcgaga
    27181 gaggatatgg aagggtgggc tgagagagtg gttagagagc aatttacgca ttaaaggaat
    27241 gactgaaaga gcctgtaaac ccttttgtgt aagtgctttt gccggtcagt taaaggtggc
    27301 catttaaacg gtcaccaaat tcgatcataa aacggttcat ggccggcttc cagttgcgga
    27361 tcggcatcgt ccatttcttg gtcgccgcct ggatagccag gtacaccacc ttcatcgctg
    27421 attcgtccgt agggaacacc ttgagttcat attaatggaa ttttctacaa atagcctccg
    27481 tggttttgag gggggattac agacgatcca tagtagtaat ccaatgagtt cttgagcgcg
    27541 gcgacatgtt tggacgcctt ggcaaaaatt agagcctgct tgaagtgcag gcgagcacgt
    27601 gcttggacga ttgatccatt cgcggtcaaa aactcaatct tggatgacag cgtgtcagga
    27661 aatccaatat cgaagtcccg ccccgtgtca aagtagggca tttcatgatc aaaggacgaa
    27721 gcttcccagg ccacttccct aatattccag ccgctagaaa caagatttcg tacgacttca
    27781 tcaacttttt tgctttccaa cttgtcacaa agtgcatagg caagagcttt ggcggcagac
    27841 acgtgcgttt cctcttaaac ataggtcgaa cggatacccg aggcaaagcg gagcattagt
    27901 cccaaagcat ctccggtaga tccggaggct ccatactgaa ggcatggcat cttgggcatt
    27961 gatatttgaa acttctctct agatagcagg gatttcttgg ccttgcctca tgctgatagc
    28021 ttgtaaatat cacacgatcc ttagccccac aaagtgagca tactccgtag ctttcattta
    28081 tagaaatcgt cgttagattg tcgcagcatg tgcacgttcc gactatttgc tcaggaagca
    28141 tcgccatacc ttggcctagg taaagaagat cagactcgta gccacatcgg tggcaactga
    28201 gcttgtacgc aatgcccatc tggttgtctc acctgaatcg taacgtcaga gctattcatc
    28261 catccattcg gcatcaatgt atgccactaa gccgaccagc tgaccgtttg gactgaagta
    28321 tgttgcctgc accccacttg acatctcatc aataccaccg cactgcgcta ggcgccggtc
    28381 ccacttgccc acagaagcgt caggatcggc tgagttcccc aagacccttg tgagccatgc
    28441 ctcctttaac tcaacctctc gaatgattgc gtcggccagc tcctccgcgt tatctagttc
    28501 gacatagtct ggaattcgga cgtacgttct gaacacagct ggatacttac ttgggcgatt
    28561 gtcatacatg acatcaacaa tgtacccgtt tgtgtaaccg tctcttggag gttcgtatga
    28621 cactagtggt tcccctcagc ttgcgactag atgttgaggc ctaacatttt attagagagc
    28681 aggctagttg cttagataca tgatcttcag gccgttatct gtcagggcaa gcgaaaattg
    28741 gccatttatg acgaccaatg ccccgcagaa gctcccatct ttgccgccat agacgccgcg
    28801 cccccctttt ggggtgtaga acatcctttt gccagatgtg gaaaagaagt tcgttgtccc
    28861 attgttggca atgacgtagt agccggcgaa agtgcgagac ccatttgcgc tatatataag
    28921 cctacgattt ccgttgcgac tattgtcgta attggatgaa ctattatcgt agttgctctc
    28981 agagttgtcg taatttgatg gactattgtc gtaattgctt atggagttgt cgtagttgct
    29041 tggagaaatg tcgtagttgg atggggagta gtcataggga agacgagctt catccactaa
    29101 aacaattggc aggtcagcaa gtgcctgccc cgatgccatc gcaagtacga ggcttagaac
    29161 caccttcaac agatcgcgca tagtcttccc cagctctcta acgcttgagt taagccgcgc
    29221 cgcgaagcgg cgtcggcttg aacgaattgt tagacattat ttgccgacta ccttggtgat
    29281 ctcgcctttc acgtagtgaa caaattcttc caactgatct gcgcgcgagg ccaagcgatc
    29341 ttcttgtcca a
//
LOCUS       DAJTSF010000045        29043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000045 DAJTSF010000000
VERSION     DAJTSF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.045"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="OO335_002106"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="OO335_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6002491.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="OO335_002107"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="OO335_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR6002492.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="OO335_002108"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="OO335_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6002493.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="OO335_002109"
     CDS             4843..5082
                     /locus_tag="OO335_002109"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002494.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="OO335_002110"
     CDS             5092..5262
                     /locus_tag="OO335_002110"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6002495.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6723
                     /gene="fdhF"
                     /locus_tag="OO335_002111"
     CDS             5311..6723
                     /gene="fdhF"
                     /locus_tag="OO335_002111"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR6002496.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6724..6990
                     /locus_tag="OO335_002112"
     CDS             6724..6990
                     /locus_tag="OO335_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR6002497.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7084..7773
                     /locus_tag="OO335_002113"
     CDS             7084..7773
                     /locus_tag="OO335_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6002498.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO335_002114"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO335_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6002499.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYRSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO335_002115"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO335_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6002500.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO335_002116"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO335_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6002501.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="OO335_002117"
     CDS             complement(10475..12193)
                     /locus_tag="OO335_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6002502.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO335_002118"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO335_002118"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6002503.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO335_002119"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO335_002119"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6002504.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO335_002120"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO335_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6002505.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO335_002121"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO335_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6002506.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="OO335_002122"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="OO335_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="OO335_002123"
     CDS             18432..18746
                     /locus_tag="OO335_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR6002507.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="OO335_002124"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="OO335_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6002508.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="OO335_002125"
     CDS             20570..21862
                     /locus_tag="OO335_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6002509.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="OO335_002126"
     CDS             complement(22016..23665)
                     /locus_tag="OO335_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6002510.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO335_002127"
                     /gene_synonym="yjcD"
     CDS             complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO335_002127"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6002511.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO335_002128"
     CDS             complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO335_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6002512.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26421..26744
                     /gene="soxS"
                     /locus_tag="OO335_002129"
     CDS             26421..26744
                     /gene="soxS"
                     /locus_tag="OO335_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6002513.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO335_002130"
                     /pseudo
     CDS             complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO335_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28753..29034
                     /locus_tag="OO335_002131"
     CDS             28753..29034
                     /locus_tag="OO335_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR6002514.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7286 a         7777 c         7352 g         6628 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcactgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctacg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcaatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtgaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgaaaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacaccaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
    25801 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
    25861 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
    25921 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
    25981 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
    26041 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
    26101 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
    26161 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
    26221 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
    26281 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
    26341 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
    26401 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
    26461 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
    26521 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
    26581 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
    26641 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
    26701 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
    26761 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
    26821 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct
    26881 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
    26941 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
    27001 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
    27061 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
    27121 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
    27181 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
    27241 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
    27301 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
    27361 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
    27421 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
    27481 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
    27541 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
    27601 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
    27661 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
    27721 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
    27781 aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
    27841 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
    27901 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
    27961 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
    28021 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
    28081 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
    28141 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
    28201 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
    28261 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
    28321 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
    28381 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
    28441 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
    28501 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg
    28561 tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca
    28621 caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc
    28681 agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag
    28741 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
    28801 agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat
    28861 gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg
    28921 gggttgcttg gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg
    28981 atgatagaag agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta
    29041 gaa
//
LOCUS       DAJTSF010000046        28914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000046 DAJTSF010000000
VERSION     DAJTSF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.046"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO335_002132"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO335_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6002515.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OO335_002133"
     CDS             complement(2138..2584)
                     /locus_tag="OO335_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6002516.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OO335_002134"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OO335_002134"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6002517.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO335_002135"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO335_002135"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6002518.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="OO335_002136"
     CDS             complement(5347..6009)
                     /locus_tag="OO335_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6002519.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="OO335_002137"
     CDS             6182..6595
                     /locus_tag="OO335_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6002520.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO335_002138"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO335_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6002521.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO335_002139"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO335_002139"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR6002522.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OO335_002140"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OO335_002140"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6002523.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO335_002141"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO335_002141"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6002524.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO335_002142"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO335_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6002525.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OO335_002143"
     tRNA            complement(9861..9948)
                     /locus_tag="OO335_002143"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OO335_002144"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OO335_002144"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6002526.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="OO335_002145"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="OO335_002145"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR6002527.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="OO335_002146"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="OO335_002146"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR6002528.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="OO335_002147"
     CDS             14005..14592
                     /locus_tag="OO335_002147"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6002529.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="OO335_002148"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="OO335_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6002530.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="OO335_002149"
     CDS             14984..15841
                     /locus_tag="OO335_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR6002531.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="OO335_002150"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="OO335_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6002532.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="OO335_002151"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="OO335_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6002533.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="OO335_002152"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="OO335_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6002534.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="OO335_002153"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="OO335_002153"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6002535.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO335_002154"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO335_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO335_002155"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO335_002155"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6002536.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="OO335_002156"
     CDS             complement(22884..24023)
                     /locus_tag="OO335_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR6002537.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="OO335_002157"
     CDS             complement(24069..26165)
                     /locus_tag="OO335_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6002538.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="OO335_002158"
     CDS             complement(26165..26911)
                     /locus_tag="OO335_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6002539.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /locus_tag="OO335_002159"
     CDS             complement(26908..27552)
                     /locus_tag="OO335_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6002540.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="OO335_002160"
     CDS             complement(27659..27964)
                     /locus_tag="OO335_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002541.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO335_002161"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO335_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR6002542.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7003 a         7320 c         7600 g         6991 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataa
//
LOCUS       DAJTSF010000047        28669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000047 DAJTSF010000000
VERSION     DAJTSF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.047"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..648
                     /locus_tag="OO335_002162"
                     /pseudo
     CDS             1..648
                     /locus_tag="OO335_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            645..1031
                     /locus_tag="OO335_002163"
     CDS             645..1031
                     /locus_tag="OO335_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002543.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1056..3269
                     /gene="tssI"
                     /locus_tag="OO335_002164"
     CDS             1056..3269
                     /gene="tssI"
                     /locus_tag="OO335_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6002544.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3292..3737
                     /locus_tag="OO335_002165"
                     /pseudo
     CDS             3292..3737
                     /locus_tag="OO335_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3759..4166
                     /locus_tag="OO335_002166"
     CDS             3759..4166
                     /locus_tag="OO335_002166"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR6002545.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4163..6451
                     /locus_tag="OO335_002167"
                     /pseudo
     CDS             4163..6451
                     /locus_tag="OO335_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            6448..7437
                     /locus_tag="OO335_002168"
     CDS             6448..7437
                     /locus_tag="OO335_002168"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002546.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7537..7740
                     /locus_tag="OO335_002169"
     CDS             7537..7740
                     /locus_tag="OO335_002169"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002547.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7758..8078
                     /locus_tag="OO335_002170"
     CDS             7758..8078
                     /locus_tag="OO335_002170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002548.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8144..8376)
                     /locus_tag="OO335_002171"
                     /pseudo
     CDS             complement(8144..8376)
                     /locus_tag="OO335_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8486..9256)
                     /gene="yafV"
                     /locus_tag="OO335_002172"
     CDS             complement(8486..9256)
                     /gene="yafV"
                     /locus_tag="OO335_002172"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6002549.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9437..9883
                     /gene="ivy"
                     /locus_tag="OO335_002173"
     CDS             9437..9883
                     /gene="ivy"
                     /locus_tag="OO335_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6002550.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9926..12370)
                     /gene="fadE"
                     /locus_tag="OO335_002174"
     CDS             complement(9926..12370)
                     /gene="fadE"
                     /locus_tag="OO335_002174"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR6002551.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12610..13188
                     /gene="lpcA"
                     /locus_tag="OO335_002175"
     CDS             12610..13188
                     /gene="lpcA"
                     /locus_tag="OO335_002175"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6002552.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13394..14161
                     /locus_tag="OO335_002176"
     CDS             13394..14161
                     /locus_tag="OO335_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6002553.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14132..14872)
                     /locus_tag="OO335_002177"
     CDS             complement(14132..14872)
                     /locus_tag="OO335_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6002554.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15256..15777
                     /locus_tag="OO335_002178"
                     /pseudo
     CDS             15256..15777
                     /locus_tag="OO335_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15953..16450
                     /gene="rayT"
                     /locus_tag="OO335_002179"
     CDS             15953..16450
                     /gene="rayT"
                     /locus_tag="OO335_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR6002555.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16667..18379)
                     /locus_tag="OO335_002180"
                     /pseudo
     CDS             complement(16667..18379)
                     /locus_tag="OO335_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18351..19136
                     /gene="lafU"
                     /locus_tag="OO335_002181"
     CDS             18351..19136
                     /gene="lafU"
                     /locus_tag="OO335_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6002556.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19207..20262
                     /gene="dinB"
                     /locus_tag="OO335_002182"
     CDS             19207..20262
                     /gene="dinB"
                     /locus_tag="OO335_002182"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6002557.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20314..20607
                     /gene="yafN"
                     /locus_tag="OO335_002183"
     CDS             20314..20607
                     /gene="yafN"
                     /locus_tag="OO335_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR6002558.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20610..21008
                     /gene="yafO"
                     /locus_tag="OO335_002184"
     CDS             20610..21008
                     /gene="yafO"
                     /locus_tag="OO335_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR6002559.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21018..21470
                     /locus_tag="OO335_002185"
     CDS             21018..21470
                     /locus_tag="OO335_002185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6002560.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21716..22759
                     /locus_tag="OO335_002186"
     CDS             21716..22759
                     /locus_tag="OO335_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6002561.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22821..23435
                     /locus_tag="OO335_002187"
                     /pseudo
     CDS             22821..23435
                     /locus_tag="OO335_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23492..24949)
                     /gene="pepD"
                     /locus_tag="OO335_002188"
     CDS             complement(23492..24949)
                     /gene="pepD"
                     /locus_tag="OO335_002188"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6002562.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25211..25669
                     /gene="gpt"
                     /locus_tag="OO335_002189"
     CDS             25211..25669
                     /gene="gpt"
                     /locus_tag="OO335_002189"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6002563.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25761..27005
                     /gene="frsA"
                     /locus_tag="OO335_002190"
     CDS             25761..27005
                     /gene="frsA"
                     /locus_tag="OO335_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6002564.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27063..27464
                     /gene="crl"
                     /locus_tag="OO335_002191"
     CDS             27063..27464
                     /gene="crl"
                     /locus_tag="OO335_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6002565.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27503..28558)
                     /gene="phoE"
                     /locus_tag="OO335_002192"
     CDS             complement(27503..28558)
                     /gene="phoE"
                     /locus_tag="OO335_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6002566.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7082 a         7123 c         7606 g         6858 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagc gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg gcctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct ttgggaacga taccttaaaa acgcaactat aacgttgaat
      601 atcataatgc ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta
      661 ttatagtgat tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg
      721 cagttgctca atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca
      781 tcttttttat gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat
      841 ttgttggtga gtattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc
      901 aagatgttcc tattaaggaa atgttctgga atttgcaggg ggatttgaat ctgacatgtt
      961 ggtttcatta taaagacggg caatggagag tgattactta tattttttgg ccaccagggg
     1021 ctagatttta atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata
     1081 aaccagttgg aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg
     1141 gtatgagcac aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac
     1201 aggctgcggc ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag
     1261 tagctgctgc gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc
     1321 aggatgcagc ggcgtcgctt gcagccggtg ctccgggggc gcataacgtc acagtaagtg
     1381 gtagtgcggt gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg
     1441 ggaatctgtt ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg
     1501 tttcccccgc tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta
     1561 tcgaactgga cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg
     1621 tcggccatga gagccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc
     1681 tgacccatac cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg
     1741 aagtactgga tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc
     1801 gcgcctggca ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg
     1861 aatgggggat ttactggtgg tttgagcaca gcgagaacag ccacacgctg gtgctggtgg
     1921 atgccatcaa tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag
     1981 ggctgaaact ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg
     2041 gaaagtgggt gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg
     2101 tggccagtcc gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt
     2161 acttcactac gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc
     2221 caggcagccg ggcacatggg gccggtcata tccgtaccct catgaccggg tacaccttta
     2281 cgttgatgaa tcacccgacg gcagaaatta atcaggaata tctgctggta cagaccactc
     2341 tgtttttacg ggataatgcg cagcacagcg gtcaggatca gcattttacc tatgtcacca
     2401 cgtttgaact gcaccccacc agcgaagttt accgcccaca acgaaccctg agcaaacccc
     2461 acaccaaagg cccgcagagc gccatcgtca ccggcccggc ggggcaggaa atctggacgg
     2521 ataaatatgg ccgggtgaag gtgcagtttg gctgggaccg ttacggaaag aatgacgaaa
     2581 acagttcctg ctggatccgc gtcagctatc cgtgggcggg taaaggattc ggcatgatcc
     2641 agatcccgcg tattggccag gaagtactgg tggatttcaa aaacggcgat ccggatttgc
     2701 ccatcatcgt ggggcgcacc tataaccagg acaccatgcc gccgtggggg ctgccgggca
     2761 tggcgtcgca gagtgggata ttcagccact cattgcaggg agggccgact aacggaaata
     2821 tgctgcgctt cgatgataaa acgggtgcgg aagaggtgaa gttccatgcg gaaaaagacc
     2881 tcaataccac ggtaaaaaac aatgaaaccc acacggtcat ggtcgatcgc ataaaaacca
     2941 ttgtcaaaaa tgaaaccatc agcgtgggag aagaccgtaa tgccaccatc acaaaaaatg
     3001 atggtctttc cgtcaaactg gctcaaacca tcaatgtcgg aacgacatat cgtctggatg
     3061 ttggcgatca atttaccctt cgttgcggca atgcagcgct tgtattacac aaggatggct
     3121 cgattgagtt ttgcggaaaa caactgttac tgcacaccag cgatgttatg caactcattg
     3181 gcagtggcat tgatatgaat ccggatggcg gcacagccat gactgcggat gatattgccc
     3241 cgttacctac ccccgacgtt tctgaataaa aaatataaaa ggaatttttc tatgaccagc
     3301 ctttaccgta ttcaggaggg ctgctttgcc ctgcctgaaa cttttcttga tcgcaccgtc
     3361 aatatcttcg tcccatcggg caatgaaagg gcaactccca gtctgaatat tttccgcgat
     3421 acgctcaggc cagatgaaaa tctgaccacc tatattgacc gccagatagc cttgatgaaa
     3481 aaaaaaacct aggccagcac cgggtacttt cgcggggaac ggcactggcg ggaactggcg
     3541 aagctgccat gacaggtgaa cagattctcg caactcatcg ttctggaaaa acagaagtgt
     3601 accagcgtca ggccgggttt atcaccgggc cagcaaaggt actaatgttg acgctgacca
     3661 ctcagcgtcc ttttgatgat cacaccgacc agctatggac tgcatggttg actagttttc
     3721 agcctgctaa aagctaaaaa aaacagggag gttttgtcat gtatgaagcc gcacgggtgg
     3781 atgaccccat ctaccacacc agcgcccttg ccgggttttt gataggggcc atcataggta
     3841 tcgccattat tgccgtcgcc agctttgctt tttttacctg cggctttttg gccgggctga
     3901 ttttgagttt tctggctgat cagatagctt ccggagtcct gcaactgggt gaggctattg
     3961 ggcgctcgat acattccaca gtgggaaaaa ttctgaccgg ttctgataat gtcagcacca
     4021 acagtcgccc ggccgcccgg gccgtactga gtacggtgat atgcgaagac cattccccgg
     4081 aaatccgcat tgcccaaggt tccggcaaca tctacatcaa cagccagctg cccgtaagga
     4141 tgaccacact gagtgtgatg cagtgattga ggacggctcc cccaatgtct ttctcggcgg
     4201 cggtacgcag acggtactgg aaatcagccc ggaaatccct gactggctgc gtcaggtggt
     4261 ggatgtgctg tatgtggtcg ccggaatgct tggcgggctg gctggcgcct ggcgtcaggc
     4321 ggcaaagatg gggtcgaaat ttggtacaaa atgtgcggca aaatttattg gtggcgaaat
     4381 ggtcgggatg gccatctctg acacagtcat gggattgttc agcaatccgg tagatgtcac
     4441 cactgggcaa aaaattttgc tgccggagac ggattttacc ctgccaggac gcctgcctgt
     4501 cacctgttcg cgcttttacg ccagccacct ggaaactgaa ggattgctgg gcagaggatg
     4561 gcggctgaac tgggagataa acctgcgtga ggatgaaaca tatatcacct tcatcggtgt
     4621 acagggtcgg gaactgagtt atccgaaaga gatgctgata ccgggccacc agatattcga
     4681 cccggaagag cagttttacc tgagccgtct gcatgacgga tgctatgtgc tgcattacac
     4741 cgactgcagc tattacgtgt ttgatgaatt tgatgaccac ggcgtcgcgc cgttactttt
     4801 tatggagacg ccgtaccggc agcgtattgc tttcgggcgt gagaacggca ggctggtgcg
     4861 ggtggcctcc agcagcggac accatctgtt attgcaccgc acgatgacgc aagcagggga
     4921 acgcctgtcg catattgaac ttttgaaggg cgggcgtccc ggtaacctgg tggaatatcg
     4981 ttacgacgat aacgggcaac tgactggcgt ggtgaaccgg gctggtgtga cggtgcgcca
     5041 gtttgcctat gaaaatggcc tgatgacaga gcatcgcaac gcgacgggct ttacctgtac
     5101 ctaccactgg gaagagattg aaggtttccc gcgcgtggtg gaacacacga ccagcgacgg
     5161 ctaggattac cggttccact atgattttgc tggcgggcag acggtggtaa ccggcaggcc
     5221 ggagcagaag tggcagtggt ggtttgatga agagacgtat gtgactgcgc accggacgcc
     5281 gggcggcgga atgtaccgct tcacgtacaa cgaaaaccat tttccggtag cggtggaact
     5341 ccccggcgaa cgcagggtaa cgctggaata cgacaccctg agccgggtgg tgaaagagac
     5401 ggacccggca ggccgggtga cacagacgca gtggaacggc agttttgctg aaatcacgcg
     5461 ccgggcgctg gatgacgacc acgtatggaa ggcggattac aacgaacacg gccaggtgat
     5521 acgggagacg gacccggaag ggcgcgtcac gcggtacggg tatgatgatc aggggctgcc
     5581 ggagacggtc tgccaccccg gtaaacagca ggaccgttac acctggaacg cgctggggtt
     5641 actgagcagc caccgtcgca tcacgggcag tgtgcagtcc tggcagtata cgcagcgcgg
     5701 gatgctggcg cgccatacgg atgaagagaa acgggagacg cgctggcagt acaccccgga
     5761 agggctggtt gccagtctga gcaacggcaa cggggctcag taccggttca gctacgacgg
     5821 cgacggacgg ctgacgggag aacagcgccc ggacggcctg ataaggatgt ttgcgctgaa
     5881 tgcggacggg tttccggtca tcatcccgac gcagggcaca gagggcgggg tgcgcaatga
     5941 acagcaggag cgtgatgcgc tgggccgcct gctgcgcagt gacacgcagc acagtacacg
     6001 gaccttcagc tacaaccgtc ttgaccagat aacggaagtg acgctcaccc ccacggaaga
     6061 aggggaacgg ctgcaccaca tgcaggcgga cacggtgcgt tttgcgtatg accgcagcgg
     6121 gtggctgacg gcagagcaca gcgtgcacgg cagtatcaaa taccggcgcg atgcgctggg
     6181 caatcccacc gatatcacgt tgccggacgg gcagcatctc agtcatcttt actacggcag
     6241 cgggcatctg ctccagacgg cactggacgg tatcacggtc agtgaatatg agcgtgacag
     6301 cctgcaccgt caggtgatac gcacgcaggg gaaactggcg acgttcagcg gctacaacgc
     6361 cgacaaccgg ttaagctggc agcgcagcct gccaggtggc agccagaatc cacagcaggc
     6421 ggtactcccc cggcgcagaa cgacagcgtg acggcgcggg actatatctg gaacgcagac
     6481 ggtgaagtcg gcggcatcaa tgacaaactg cgcggttgcc tggtgttcag ctatgaccgc
     6541 agcggctggc tgaccagccg cacggggcag atgtatgacc atgaccatta ttattacgac
     6601 aaagcgggca acctgctgac ggatgagtat cagggagcgg tgatggataa ccgcctgccg
     6661 ggttacgggc gtgaccgcta ccgctataac gaatggggcg aactgacaga gcgcagggac
     6721 cagcagctgg agtggaacgc gcagggccag ttaacgcggg tcatcagcag caacagcgag
     6781 acgcggtatc agtacgatgc gctgggcagg cgcatcagca aggcgaccag caatctccat
     6841 accgaccggg gagaacgaag ccggacaacg tttgtgtggg aagggttccg gctgttacag
     6901 gagacaacgt ggcagggaaa gcggacatac ctgtacgatg cggaacagcc gtacacgccg
     6961 gtggctgcca tcacggggcg cggagagagc cagaaaatct ggtattacca cacggacctg
     7021 acgggcacgg tacacgaggt aaccgcgccg gacgggacgc tggtgtgggc gggctaccag
     7081 gcgggatttg gtgaaaaccg gggcgacatc agcaacagcg gggcgtactt tgagcagccg
     7141 cagcggctgc cggggcagta ctttgacgaa gaaacagggc tgcattacaa tctgttcagg
     7201 tattatgcgc cggagtgcgg gaggttcgta agccaggacc cgataggatt aaacggtggg
     7261 ttgaacttat atgcttatgc gcctaatccg ttaggttgga tcgatccgct aggactgaat
     7321 tctttaggaa tagattcaaa taatatattt agaggagatg gtaactataa aggtggcgga
     7381 attggacagc caaagagtgg cacaatatca gcacaggaca ttattgatca tctctaagaa
     7441 aaaggtgatg ggcgtttaac atcttttagt actaaaaaaa catctagttc caactctcgt
     7501 ggtgcagatt ttttctcaaa aaaacatgtc ttcaaagtgg cagtttctga tctcgatgat
     7561 ttagttaaaa ataatcagat cactgtttta acacaagaaa aagtaaaagc aatatttggt
     7621 ttagaatcaa gcaagaaaat tagaaaagaa cttggtaata ttcttcagaa tatggctagg
     7681 aataatgagg tgttagttca tgggcaaatt cttggaaata aagttagccg atgtaattag
     7741 ttagtggaga ggaagttgtg gacgaaatta gaataagaat tgctggtaat acggctgcgc
     7801 cttgtttcca tgtgctaaaa aataagggat ataaagtaac aatggaaact catgcattat
     7861 ctgacgattg tttagattgt gtatatgatt ggtatgcgga aaaaaatgga caatttttta
     7921 gtgcaacgag tccagaagag ttacttggat tagttgcaat gtgggaagtt cgtggtaatg
     7981 actggagagc taatttgaat gagataaata cttatcaaaa agccatagaa gaatccccgt
     8041 tttttgataa aaacggcaat gaaatagagt ctgactaaat tttattgata tagcaacgat
     8101 aataaccgga aatttcttcc agatctttcc ggctttttat tatttaactt tcgttcagca
     8161 cactaaacat cccgtctcgc atccccttaa ctggctgttt aacctgataa agttccctgc
     8221 gcagttcctg tacttcgttg ttatcccaag caggatcaaa catccctccg agatcttcac
     8281 ggtaacgccc gtgccgatct caaatcccgc ttttctcagc catttggcgc tgattttgat
     8341 caccggcgtg tcgctgcggt tgggcagata acccacggta cagcgacgct gtgttttgga
     8401 aatggtgact tctggcgtaa tattgtgttt aagttagggg ctttcgtaat ctagcccagc
     8461 ccccggccct aaacacaacc cccactcacc acaacctaaa ctcatccgca tcccgccatg
     8521 ccggaaactt ttctctatat tcccgcaatg ccaccatcga cagctccgca tcaatacgcg
     8581 ttgccttatg cgcgtcggcg gtagcgataa tctcgccttg cggattaatc acccggctgt
     8641 caccgcgata atggcagccg ttgccatcgc tgccgacgcg attgcatccc gccacatacg
     8701 cctgattctc aatcgcgcgg gccgtcagca atgcctgcca gtgcagagag cgcggagctg
     8761 gccagttggc gacgtacagg gcgaggtcat aatcgttgag attgcgcgac cacactggaa
     8821 aacgtaagtc gtagcacacc agcggcaaaa tacgccagcc gcgccattcc acaatcactc
     8881 gcgcattgcc cgctttataa tgcagatgct catctgccat gcggaacaga tgacgcttat
     8941 cataaaaatg taccgtgccg cccggctcaa ccagcaaaaa gcggttaacc gaaccagact
     9001 ccgtttgtaa tgcaacactg cctgcaatca gcgcattgca ctgctgcgcc ttagctgtca
     9061 tccagttcac tacgtcatct tgtgcaagcg acgaagctgc cgcttccatg gcaaagccgc
     9121 tggtaaacat ctccggtaga acgatcacat cgcgcccggt aataccttcc agttgacgat
     9181 caaaatggcg caggttggca ggaccatcca cccacaccaa cggttgctgc aaaagcgtaa
     9241 tcttcaaact aggcacggtg tacaactcct ttatgcgaag ggttttataa ctttaacacc
     9301 ttatcaggca gttgccttag cgcagaataa attgataaca aatgctgata ttggaaatat
     9361 ctgatttgca aattatcgtg ttatcgctag gctttaggag gttaataaca tgggcaggat
     9421 aagctcggga ggaatgatgt ttaaggcaat aacgacagtc gccgcactgg tcatcgccac
     9481 cagtgcaatg gcgcaggatg atttaaccat tagcagcctt gcaaagggcg aaaccaccaa
     9541 agctgcgttt aatcagatgg tgcaagggca taagctacct gcatgggtga tgaaaggcgg
     9601 tacttatact cccgcacaaa ccgtaacgtt gggagatgag acgtatcagg tgatgagcgc
     9661 gtgcaaaccg catgactgtg gctcgcaacg tatcgctgtg atgtggtccg agaaatctaa
     9721 tcagatgacg gggctgttct cggctattga tgagaaaacg tcgcaagaga aactcacctg
     9781 gctgaatgtg aacgatgcgc tttcgattga tggtaaaacg gtgctgttcg cggcgttgac
     9841 cggcagcctg gaaaaccatc cggatggctt taattttaaa taattagcgg ataaagaaac
     9901 ggagcctttc ggctccgtta ttcaattacg cggcttcaac tttccgcact ttctccggca
     9961 actttaccgg cttcgtcgcc agctcttccg gatcaaagtc atcaacgtta atactgcgca
    10021 gacggctttc ttccgctttc accagaatag cggcttcatc tttatcaatc agccccttcg
    10081 tcagcgcgtt gtgcgccagt tcatccagac gggtaaatgg cagattttta cccagctctt
    10141 tacagatccg ctgatgaatc gggtcggcgg caatcacatc caccaacgcc tcttccagta
    10201 aaccaaccgg gttatgctcg cttggcgtca ggtactgacc gcgaccaata cgtgaacgag
    10261 tggcgttcgg cacttgtaaa atcttcgcca ttttatgatc cagcttgtca gacggtgcca
    10321 gataatgacg tccggtcggg aagatcacca cattcagcag cccggcaacc acgcggttcg
    10381 ggaagttttg cagtaaattg tccattgcct gttctgcctg atacagcgca tcctgtacgc
    10441 cccagtgcac cagcggcaga tcggcttcat tacggccttc gtcgtcataa cgcttcagaa
    10501 cggcagaggc gaggtaaagc tggcttaaaa tatcccccag acgtgccgag atacgctcac
    10561 gacgtttcag gctgccgccc agcagtgcca tcgagacatc agaaagcagg gcgaggttgg
    10621 cgctcaggcg gttcaggtgc tgatagtagc gtttagtggc atcgccggtt ggcgtgctgc
    10681 tggttaaacc gcgcgtcagg cccagccaga agctgcgaac tttgttgcta ccgacgtgac
    10741 cgatatgttt gaacaacagt ttatcgaacg cgttgacgtc attgttcttc gccgcttcca
    10801 tctcttccag cacgtacgga tggcaacgaa tcgctccttg tccgaagatc atcatgctgc
    10861 gggtcagaat gttagcccct tcaacggtga tggcaatcgg tgcgccctgg taagcacgcg
    10921 ccaggaagtt gctttgcccg agcataatgc ctttaccgcc ggtaatatcc atcgcatcaa
    10981 taatcgactg ctgcccgcgg tgggtacagt gatacttaac gatagccgac agcacggcag
    11041 gtttttcgcc gagcataatg ccgtaggtaa tcagcgatgc cgcagcatcc attacataag
    11101 cgttcccggc aatacgcgcc agcggctctt caatcccttc catcttaccg atagagattt
    11161 tgaactgacg gcgaatgtga gaatacgcgc ccgttgccag cgctaccgat ttcacgccgc
    11221 cggttgagtt ggaaggcagg gtgatgccgc ggcctaccga gaggcactcc accagcatcc
    11281 gccagccttg cccggccatt ttcggcccgc cgatgatgta atcgatcggc acgaagacat
    11341 ctttaccgcg cgtcggtccg ttctggaacg gtacgttcag cgggaagtgg cgacgaccaa
    11401 tttccacgcc cggcgtggtg gttgggatca gcgcacaggt aatgcctaaa tcttctgcac
    11461 cgccgagtaa tttttccggg tcggagagtt taaacgccag cccaagcacg gtcgcaatcg
    11521 gtgccagcgt aatgtagcgt ttgttccagg tcagacgcat ccccagcacc tgctggccct
    11581 gccattcgcc catgcagaca atcccggtgt ccggaatcgc gcccgcatcg gaacccgctt
    11641 ccgggctggt cagtgcaaag caggggatct cctgaccacg cgccagacgc ggcagatagt
    11701 gatctttctg ctcgtcagtg ccgtaatgtt gcaacagttc gcccgggcct aatgagtttg
    11761 gcacgccgac ggtaatcgcc aggatcccgc tcacgccaga gagtttttgc agcacacgag
    11821 actgggcata agctgagaac tccagtccgc cgtactcttt tttgataatc atcgcgaaga
    11881 agcgatgctc tttaaggtac gcccacaact ccggtggcag atccgccagc tcatgagtaa
    11941 tctggaaatc attcgccatc cggcaggctt cttctaccgg gccgtcgaga aacgcttgct
    12001 cttcagcggt caggcgcggc tgcggatagt tatgcagctt tttccagtcc ggcttgccct
    12061 ggaacaagtc gccctcccac caggtggtgc ccgcatcaat cgcttctttc tcagtgcgcg
    12121 acatcggcgg catcacctta cggaaaccgc gaaataccgg cgcggaaatc atcgacttac
    12181 gcataggcgc aaagttaaat ggcacgagga taatggtcag aggcaccagt acccacgccg
    12241 accacagacc agcaacgccg agggcggctg tccaggcgag caaaatcaga ctgctgataa
    12301 ataagctcac gcggtgatag aacaacgcgc cgagcaggac aaccgtagcg agaatactca
    12361 aaatcatcat aacgaaaagc cccttacttg taggaggtct gaccacttgt gatgatatgg
    12421 ttgtagtgga tgtaaaaaca tttagcaatg tgtttacaat ataattacaa caaagctcac
    12481 attgttgctg tttttatccg cacttcaggt caaaaagtcc tggtcatagc acctgcccgt
    12541 acttctcgct tttggcggta tccggtacac tgcattttgt ctattacatt tatgctgaag
    12601 gatatcctca tgtaccagga tcttattcgt aacgaactga acgaagcggc ggaaacgctg
    12661 gctaactttt taaaagatga cgccaatatt cacgccattc agcgcgcggc ggtcctgtta
    12721 gcagacagct ttaaagccgg tggcaaagtg ctttcctgcg gcaacggcgg ttcccattgc
    12781 gacgctatgc actttgccga agagttgacc ggtcgctacc gtgaaaaccg tccgggctac
    12841 ccggcgattg ctatttctga cgttagtcat atttcctgcg tcggtaatga tttcggtttc
    12901 aatgatattt tctcccgcta cgttgaagcg gtaggtcgcg aaggcgatgt actgctgggg
    12961 atctccacct ccggtaactc tgcaaacgtg atcaaagcga tcgcagcggc gcgtgagaag
    13021 gggatgaaag tgatcaccct gaccggtaaa gacggcggta aaatggctgg cacggcggat
    13081 atcgaaattc gcgttccgca ctttggttat gccgaccgca ttcaggagat tcacattaaa
    13141 gtgatccata tcctgatcca gttaattgaa aaagagatgg ttaagtaagt ctggcgtagg
    13201 ccggataagg cgtttacgcc gcatccggca cttgtgcgct gatgcctgat gcgatgctga
    13261 cgcgtcttat catgcctaca aatctgtacc cgaaccgtag gccgaataat tcgtttacgc
    13321 cgcatccgac ctgaaaattc ttaaatcaat cttcgccggg gtccatgcgc tcccgctgtt
    13381 gtggaggtta cccatgtgcg aattgctcgg gatgagcgcc aacgtcccta ccgatatctg
    13441 ctttagtttc accggacttg tacagcgtgg tggtggaacc gggccacata aagatggctg
    13501 gggcattacc ttttacgaag gtaaaggctg tcgcacattt aaagatccac aacccagctt
    13561 taattccccc atcgccaaac ttgtccagga ctacccgata aaatcctgtt cggtggtggc
    13621 tcatattcgc caggctaatc ggggcgaggt ggcgctggaa aatactcacc catttacccg
    13681 cgagttatgg gggcgtaact ggacttatgc ccataacgga caactgacgg gctacaaatc
    13741 actggaaacc ggcaacttcc gcccggtcgg tgaaaccgac agcgaaaaag ccttttgctg
    13801 gctgctgcat aaattaacgc agcgttaccc gcgcacgccg ggcaacatgg cggcagtgtt
    13861 taaatatatc gcctcactgg cggatgaact gcgggcgaag ggcgttttca acatgctgct
    13921 ttcggacggg cgctatgtaa tggcgtattg ctcgactaat ttacactgga tcacccgccg
    13981 cgcgccgttt ggcgtggcaa cgttgctgga tcaggatgtg gaaatcgact tcagctcgca
    14041 gaccacaccg aatgatgtgg tcacggtgat tgcgacacag ccgctgacgg gcaatgaaac
    14101 ctggcaaaaa attatgccag gcgaatggcg cttattttgc ctcggggagc gtgtagtttg
    14161 atgccagttg tggctgcacc acttcgtggc ttaacggctt gctgaccacg taacggccat
    14221 tggccacaga aacggtcggt ggcttacggg tttgctcaaa gtagtcgtag cccggcttca
    14281 gctgctccca gaagtcctta aagttggaat atttatggcg cttcatattg gcgtcggtca
    14341 tgcggaatgg gtaaatactc acctgcacgc tcggctgacc aaacaccagc gcaccagtaa
    14401 cgaactggaa gatctcatca ataccctgat tggtcattgc gtagcagccg atggatacac
    14461 aatcgccgtg gatcatcagg tatttccctt cataaccatg cgcacggtca taagcattgg
    14521 ggaatccaat attaatcgct ttgtagtaac ggctgtctgg ttttaactga ttacgctgga
    14581 cgctataaaa cccttccggg cttttgaaat cgccctgacg ctgttttggc cctaagccgc
    14641 cggaatattt acagatttta taactgtcga gcagttgata ttgctcgccc attttgacgt
    14701 aaagatcgag cgtacgttct tccttgaaga tctggatgta gacaggggat cccatcaact
    14761 gctgcttata ctctttgctc actggcgtgg tcgagctact gctgcccagc aaaccggcaa
    14821 acgaaacgca cgggatcaac aacatcgcaa gaattaatgc gattttacgc atactgctta
    14881 ttccttgata aaacggttac acacgccagg acggcaaaat ggatcccaaa tcggaatagt
    14941 ctggatttgg aaggctcaca ttatcaccaa aagagtttta cgcaagcctg tcggcgctgg
    15001 gtttacaaat ttagcacagg ggcagttttc aatagtccga aaagcgcatg tatcatttat
    15061 ttcctaatat tatgaaatct caatagtaat atgcaaataa gcaattaata atcccgttta
    15121 ctcctccggt cttttaaaat tctcaccttc attcagccgg tttatgtctt tcatgtcttc
    15181 gttctggctc ggcagatttt tgcatccagg cgtgttttcg ttatgcgttt tgctcccttt
    15241 actcgccagc gccaccacaa ccgggagcag ataaaacagc atccagaatg gtttcctgcg
    15301 ccgctcaagg cgagtgacag acgctggcag gcgctggcgg aaaacaacca ctttttaagc
    15361 agcgatcacc tgcataacat caccgaagtg gcgattcacc gcctggagca gcagcttggc
    15421 aagccttacg tctggggcgg cacgcggccc gatcaaggct tcgattgtag tgggttagtt
    15481 ttttatgcct acaacaagat ccttgaggcg aaactcccgc gcacggccaa tgagatgtac
    15541 cactaccgtc gggcaacgat tgtggcgaat aacgacctgc gccggggaga tttgctattt
    15601 ttccatatcc acagccgcga gattgccgac catatggggg tgtatctggg cgacggtcag
    15661 tttattgagt cactgcgcac cggggaaacc attcgggtaa gccggttagc cgaacctttc
    15721 tggcaggacc attttttagg cgcgcgcagg attttgacgg aagagatgat tttgtaggcc
    15781 ggataaggcg tttacgccgc atccggcggt tgtgcacgga tgcctgatgc gacgctgacg
    15841 cgtcttatca tgcctacaaa tctgtgcgtt tttgcgagcc gctttcccga tataacacag
    15901 cgtcattgtc ggcctttcct tttcctctta ccgttgagaa aaaggagtcg ctatgtcgga
    15961 atatcgtcgt tattacatca aggggggaac atggtttttc acggtaaatt tacgcaatcg
    16021 tcgaagccat cttctgacca cccagttcca gacgctccgt aacgccatta ttaacgttaa
    16081 gcgagacaga ccttttgaaa ttaacgcctg ggtcgttttg ccggagcata tgcattgcat
    16141 atggacatta cccgaaagcg atgatgattt ttcctctcgc tggcgggaga ttaaaaagca
    16201 gttcacccat gcttgcggat tgaaaaatat ctggcaaccg cgtttttggg agcatgccat
    16261 tcgcaataca aaagattatc gccatcatgt tgattatatt tatataaatc cagagaaaca
    16321 tggctgggta aagcaggtga gtgattggcc attctcaacg ttccatcgcg atgtcgcgcg
    16381 agggttatat cccatcgatt gggcgggaga cataacggat ttaagtgccg gggagcgtat
    16441 cattttataa ctgtgcgctg gtgccggatg cgcttacgct tatccggcct acaaatcgtg
    16501 ctttaacggt aggccggata aggcgttcac gccgcatccg gcagttatgc gcagatgcct
    16561 gatgcgacgc tggcgcgtct tatcatgcct aaaaacttgt gcctgaacgg taggccggat
    16621 aaggcgttta cgccgcatcc ggcaattgtg caccgatgcc ggattcttac atcaacgccc
    16681 ccataatctt caactccagc tcatccggca cttcgttata cgacagcaca tgcagccccg
    16741 gcgcaaacaa ccttgcataa cgcggcagca aagggcgtag ctgcggcggc accagcagca
    16801 ccggatcttt ccccgccgct ttcatctgct ctttcacctg tggcatcgtg ctctggaact
    16861 ggttgagcat attcgggtca accggcacgc tgtcgagcat cactttgccg gcctgttgcg
    16921 cctgattcac tacgttggtc agcagatttt ccagctcatt attcagcgta tacaccgtca
    16981 gctcctgctt gcgaacgaac ggatgagtaa tgctgcgccg cagcgccagg cgcacatcgg
    17041 ccgccagcag aatatgatct ttagtcaccg tgctactggc gaccagcacg gtggcgatgg
    17101 tgacgatatc gcgcagggaa acgccttcgg tcagcagcgc ccggtacact ttcagcaatt
    17161 ggctgtaatt gagcgccgcg cttaaatcct ccgccaggcg cggtgccatc gacgacaaac
    17221 ggttatgcaa ctgcgtgatg tcatcatagt taaacaaatc aggaatatag ctgcgcacaa
    17281 tcttgttcac atgggtggca atcacactgg cgctgtcgat cacctgatac cccatattca
    17341 gagccttcgc tttctgcgca ggctggatcc aggtgaccgg tatcccgtac gccgggtcgt
    17401 tccccagcac gccgtcaatc tcgccgtagg tttcgctgga gggcagcgcc atcagtttat
    17461 ccgccggaat atccgcttca tcagcctcaa tgccgttgat gaaaatagcg tactggctgg
    17521 gcttgaggcg gaagttttcc cgaatgcgga tctccggcag cagcacgccg ttaccgtcgg
    17581 aaatcacctg ccgaacgccg cgaatccgct gggtgagcgg gttgccctgc gctttgtcca
    17641 ccagcgccac cagcttgtag ccgaggctta agctgatggg ctcgatcagc ggaatagttt
    17701 cccaactgac ctgttgttcg ctggtttcag tgatggtgcg ggtcagcgtt tcgaggcttt
    17761 tctcttccgc ctccgccgcc tgcgggcgtt tgctcatccg ccagccggta aagccaagca
    17821 gggcgctgaa cagcaaaaac ggcagatgcg gcatccccgg caccaccgcc agcacgaaca
    17881 tgatcccggt ggcggtgtag agcaccgacg ggcttgccag caactggtgg cgcacgtcat
    17941 gggcgatatc gccgttgtcg ctgacgcggg tgacgataat cgccgccgcg gtggagagca
    18001 gcagggaagg gatctgcgcc accaggccgt cgccgatggt catcagcacg tattgctgga
    18061 aggcggcgtc ggcgctcagg ttgtatttga agatgccgat acagacgccg ccgctcaggt
    18121 tgatcgccag aatcatcatc ccggcgatgg cgtctccgcg tacaaacttc gatgccccgt
    18181 ccatcgcgcc gtagaaatcg gcctcgctgg caacatcttt acgccgggtt tgcgcctgcg
    18241 cctggttgat caatccggcg ttaagatcgg cgtcaatcgc catctgtttg ccgggcatcg
    18301 cgtccagggt aaagcgggca gaaacctcgg aaatacgctc ggcctctttg gtgacgacga
    18361 taaagttgat tgtcaacagc gtcagtaaat ccgagcgtga aagcattatc gccgcgctgc
    18421 acgggcagtc gatttttaac ggtggtgggc tgtcgccgtt aaataaaatc agcccgtctc
    18481 atccgccaaa acctgccacc gtggcggtgc cggaagagac ggagaaaaaa gcgcgtgatg
    18541 tgaatgagaa aacggcgctg ctgaagaaga aatccgccac cgagcttggc gagttggcaa
    18601 ccagtatcaa caccattgcc cgtgatgcgc atatggaagc gaatctggag atggagattg
    18661 ttccccaggg attacgcgtg ctgattaaag acgaccagaa tcgcaatatg ttcgaacgcg
    18721 gcagcgcgca gattatgccg ttctttaaaa ctttgctggt ggagctcgcg ccagtgttcg
    18781 actcgctcta taacaaaatt attattaccg ggcataccga tgcgatggcc tacaaaaaca
    18841 atatctacaa caactggaac ctttcgggcg atcgcgcgct ttcagctcgt cgggtgctgg
    18901 aagaggccgg aatgccggaa gataaagtga tgcaggtaag cgcaatggcg gaccagatgc
    18961 tgctggacgc gaagaatccg caaagcgcgg gcaatcggcg tattgagatt atggtgctga
    19021 ccaaaagtgc gtccgatacg ctgtatcaat actttggtca gcatggggat aaagtggtgc
    19081 agccgctggt gcaaaagctg gataagcagc aggtgctttc gcagcgaatg cgctaaatgc
    19141 tgaatcttta cgcatttctc aaaacctgaa atcactgtat actttaccag tgttgagagg
    19201 tgagcaatgc gtaaaatcat tcatgtggat atggactgct ttttcgcagc ggtggagatg
    19261 cgcgacaatc ccgctctgcg cgatatccct attgctattg gcggcagccg cgaacgtcgg
    19321 ggggtgatca gcaccgccaa ttatcccgcg cgtaaatttg gcgtacgtag cgctatgcct
    19381 actgggatgg cgctcaaatt atgcccacat ctcaccttgc ttccggggcg ctttgacgcc
    19441 tacaaagaag cctcaaatca tatccgtgaa atcttctcgc gctacacctc gcgcattgaa
    19501 ccgttgtcac tggatgaggc ttatctcgat gtcaccgata gcgtccattg ccacggttct
    19561 gcgaccctca tcgcccagga aatccgccag acgatcttca acgagctgca actgacggca
    19621 tctgcgggcg tggcaccagt aaagtttctc gccaaaatcg cctccgacat gaataaaccc
    19681 aacggtcagt ttgtgattac gccggcagaa gttccggcat ttttacaaac cttaccactg
    19741 gaaaaaatcc ccggcgtcgg caaagtctcg gcggcaaaac tggaagcgat ggggctgcgg
    19801 acctgcggtg atgtacaaaa gtgtgatctg gtgattctgc ttaaacgctt tggcaaattt
    19861 ggccgcattt tgtgggagcg tagtcagggg attgacgagc gcgacgttaa cagcgaacgg
    19921 ttacgaaaat ccgtcggcgt ggaacgcacg atggcggaag atattcatca ctggtctgaa
    19981 tgtgaagcga ttatcgagcg gctgtatccg gaacttgaac gccgtctggc aaaggtaaaa
    20041 cctgatttac tgattgctcg ccagggggtg aaattaaagt tcgacgattt tcagcagacc
    20101 acccaggagc acgtctggcc gcggctgaat aaagctgact taatcgccac cgcgcgtaaa
    20161 acctgggatg aacgccgcgg cgggcgcggt gtgcgtctgg tggggctgca tgtgacgttg
    20221 cttgacccgc aaatggaaag acaactggtg ctgggattat gatgtatact attatgtata
    20281 ttctggtgtg cattattatg agggtatcac tgtatgcatc gaattctcgc tgaaaaatcg
    20341 gtcaatatca ctgagttacg taaaaaccca gctaaatact ttattgatca accagttgcg
    20401 gttctttcta ataatcgccc cgcaggatat ctcttaagtg ccagcgcatt cgaagcgtta
    20461 atggacatgc ttgctgaaca agaggagaaa aagcccataa aggcgcgctt tcgtccaagt
    20521 gctgcaagat tagaggaaat tacacgccgc gctaaacaat atcttaatga tatgacggat
    20581 gatgatttca atgactttaa ggaataagga tgcgggtatt caaaacaaaa cttattcgcc
    20641 tgcaacttac agcagaggaa cttgatgcgt taacggcgga ttttatttcc tataagcgtg
    20701 acggtgtttt gccagatata tttggtcgcg atgcactcta cgacgactcg tttacctggc
    20761 cattaatcaa atttgagcga gttgctcata ttcatctggc aaatgtgaat aatccatttc
    20821 cgccacagtt gcgccaattc agcagaacga atgacgaagc gcatttggta tattgtcagg
    20881 gtgcgtttga tgagcaagca tggttgctca ttgccattct gaagcctgaa cctcataaac
    20941 tggctcgaga taacaaccaa atgcataaaa ttggaaaaat ggcagaagcg tttcgcatgc
    21001 gtttttgaat ttatattatg aataacatac aaataagaga ttatcagcct ggcgattttc
    21061 agcaactatg cgctattttc attagagcgg ttacgatgac cgtcagtcag cattattcac
    21121 tacaacaaat tgccgcctgg gcgcagattg acgaatctcg ctggaaggag aaactcgcga
    21181 aatcacaagt gcgggttgca gttattaatg caaaactggt cggctttatt acctgcgttg
    21241 aacactatat cgatatgtta tttgttgacc ctgaatacac ccgccgtggg gttgccagcg
    21301 ctttgttaaa accttttatt aagtctgaat ccgaacttac ggtggacgca agcataaccg
    21361 caaaaccctt ttttgaacgt tatggttttc agacagttaa gcagcagcgc gttgaatgcc
    21421 ggggagcgtg gtttattaat ttttatatgc gttataaacc gcaacattaa atccagcttg
    21481 caatgaaaat aacgcccgcc tagtatgtgc gcgttttctt attccacaaa ctgcaaggag
    21541 gtaaatcatg acaaatcctt tatcaatgac tctttgcaga cctttccagg attaattctt
    21601 tttttcttgc cctggattcg tctgccattt cctgattttt taattgttac tctcaataat
    21661 tcaagtcgca gcacatgcaa cttgaagtat gacgagtcta tttacggaat ggtatatggg
    21721 caattatatt cgtcccttat ccgatgcggt atttaccatc gcatctgatg acctgtggat
    21781 cgagagttta gcgatccaac aattacacac cacggcaaat ttacccaaca tgcagcgcgt
    21841 agtggggatg ccagatttac accccggacg cggctacccg attggcgcag cgttcttctc
    21901 cgcaggccgt ttttacccag cactggtcgg caatgatatc ggctgtggca tggcgctatg
    21961 gcaaacagat attctcgctc gcaaatacaa cgccgataag tttgaaaagc gattatcagc
    22021 tttggatgac gttgctgaag aaagctggct ggaggaaaac ctgccgtcag catttgcaca
    22081 gcatccgtgg cgcagctcgc ttggctctat cggtggtggc aatcatttcg ctgaactgca
    22141 acaagttgat caaatcatca acgctgaact gtttgcactg gcaggtctgg atgcgcagca
    22201 tctgcaactg ctggttcata gcggctcgag ggggctaggg cagtctattt tacagcggca
    22261 tattgcctcg ttttcgcatc atggtttgcc tgaaggcagt gacgacgcgc tggcgtttgc
    22321 gcgttttaat cgccacctga tcgctttgcg cataatgcaa caggttaagg ccaccggaag
    22381 tccggttctg gatgtggcgc ataactttgt tagcgcgtgt cgaatcggtg atcaacaagg
    22441 ggtcttacat cgtaaaggtg ccacgcctga tgactgtggt ctggtggtta ttcccggttc
    22501 acgcggtgat tactccaggc tggttcagcc cgtcaggagt gaggaaacat tgcattcgct
    22561 ggcgcatggg gctgggcgta aatgggggcg caccgagtgt aaagggcgtc tggcagcgaa
    22621 atacacagcg acgcaactct cgcgtactga acttggcagt cgggtaattt gtcgcgataa
    22681 acaactcatc ttcgaagaag cgccacaagc ctataaatcg gctgaaagtg tggtgcaatg
    22741 tcagggcagg ccggattaat tattcctgtc gcgcggctgc gtccggtgct aacgctcaaa
    22801 aacagtggag ggaaaaaagg atgatcttgc tataactctc ctcagcgcag gggccggaag
    22861 aatgttgtct cgcagtgaaa aaggcgctgg acaggctaat taaagaagct gcccgacaag
    22921 acgtcgcggt aacggtgctg gaaacagaaa caggccgcta ctctgacacg ctgcgttcgg
    22981 cgctgatttc tctggatggc gacaacgcat gggcgttaag cgaaagctgg tgtggcacta
    23041 ttcagtggat ttgcccgagc ccgtatcgcc cacatcacgg gcgtaaaaac tggtttctgg
    23101 gcattgggcg ttttaccgct gatgagcagg agcaatccga tgcgatccgg tatgagacgc
    23161 tgcgttcgtc ggggccgggc ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca
    23221 cgcatttggc atccggtatt agcgtgaagg ttcagtcaga gcgcagccag catgctaaca
    23281 aacggctggc gcgattgctg attgcctgga agctggatca gcagcaatag gaaaatagcg
    23341 cggtgctgaa atcacagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgcgac
    23401 gaacgtttac agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg
    23461 attaaacgcc atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt
    23521 cagtcagcag tgtccagtaa tgacctacgc tttcgatgtg aacttgctca tccggagagt
    23581 gtggaccggt gatagttggc ccgatagaaa ccatgtccat ttccggatac ggttttttga
    23641 acagaccaca ttccaggccc gcgtggataa tctggatgtt cggcgtcttg ttgaacaggc
    23701 gctgataggt ttcacgtacc agatgcatca ccggagaatt agcgtccggc tgccagccag
    23761 gatatgcgcc tttcgcttcg gttttcgcgc cagccagttt acccagcgaa tccagcatgc
    23821 tcaccacgta gtctttaccg ctgtcgatta gtgaacggat caggcagtga atttctacgt
    23881 tattgtcagt catggtcacc acaccgacgt tcagggaggt ttcgaccaca ccttttgcca
    23941 cgtcggaatt gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc
    24001 gagatttcgc aatcagggca gctttatcgt tcgctacaga gtccagcaac aaggccagat
    24061 ttttctcttt ctctgccagc tcgtttttca ggatctcctg ataggtattc accagagatt
    24121 tcagtgcgtc gactttatca gctgcgacag caatggtcgc aaaggcttca cgcgggatgg
    24181 cgttacgcag tgtgccgccg ttgaaatcga taaggcgcag gtccagttct tctgcatgac
    24241 ccgccaggaa gcgcaccagc agtttgttgg cattacccag gccaacgtgg atttcaccgc
    24301 cggagtgacc gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa
    24361 ccgcttcacg atctaaatgc aggttggagg tgaagtcgat accccccgca caacccatgt
    24421 agatttcacc ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc
    24481 tctgtaagcc gaacgcaccg tccataccgg cttcttcggt catggtcagc agcacttcca
    24541 gcgggccgtg aaccacgttt tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc
    24601 cgttatccgc acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct
    24661 ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga
    24721 ggtgggcctg taagacgacc ggtttacgat tttccatacc tgcggtagca ggttgacgaa
    24781 tcaggatatt acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa
    24841 caatgtattc agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg
    24901 caaaaatatc ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc
    24961 ttgtcgatca cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac
    25021 acaagacagg cttgcgagat atgtattgag aataccactt tatcccgcgt cagggagagg
    25081 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
    25141 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
    25201 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
    25261 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
    25321 tggcggtctg gtaccggggg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
    25381 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
    25441 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
    25501 tgttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
    25561 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggatcgaaca
    25621 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
    25681 cctggcattg ccgggcgttg ttctttttaa cttcaggcag gttacaatag tttccagtaa
    25741 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
    25801 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
    25861 ctgtgcagtc ggctcttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
    25921 tgatgtggat ctggcgcggt attgacccac gtgaaatcct cgacgtccag gcacgtattg
    25981 tgatgagcga tgctgaacgt accgacgatg atttatacga taccgtgatt ggctaccgtg
    26041 gtggcaactg gatttatgag tgggccaccc aggcgatggt gtggcaacaa aaagcctgta
    26101 cggaagaaga tccgcaactc agtggtcgac actggctgca tgcggctacg ttgtacaaca
    26161 ttgccgccta tcctcatctg aaaggagatg acctggccga gcaagcgcag gctttatcaa
    26221 accgcgccta tgaagaggcc gcccagcgtc tgccgggcac gatgcggcag atggagttta
    26281 ccgtacccgg cggtgcgccc atcaccggct ttttgcatat gccgaaaggc gatggcccgt
    26341 tcccgacagt attaatgtgt ggtggtctgg atgcgatgca gacggactat tacagcctgt
    26401 atgaacgtta ttttgcgccg cgcggcattg cgatgctgac tattgatatg ccgtcggtgg
    26461 gcttttcttc aaaatggaag ctcacccagg actccagcct gttgcatcag cacgtcttaa
    26521 aggcgctgcc taacgtaccg tgggtggatc acactcgcgt cgcggccttt ggtttccgtt
    26581 tcggcgctaa cgttgccgtg cgtctggcat accttgaatc gccgcgtctg aaagcggttg
    26641 cctgtcttgg tccggtagtt cataccctgt tgagtgattt taagtgccag caacaggtgc
    26701 cggaaatgta tcttgacgtt ctggcgagtc gtttggggat gcatgatgct tccgacgaag
    26761 cgttacgcgt ggagctgaat cgctattcat taaaagtgca aggattgctg gggcgtcgct
    26821 gcccaacgcc aatgttatca ggctactgga agaacgatcc gttcagcccg gaagaggact
    26881 cacgcttaat cacctcatca tctgctgacg gtaaattatt agagatccca tttaacccgg
    26941 tgtatcggaa ttttgacaaa ggtcttcagg aaatcaccgg ctggatcgaa aaacgcttgt
    27001 gttaaaaatt tgctaaattt tgccaatttg gtaaaacagt tgcatcacaa caggagatag
    27061 caatgacgtt accgagtgga cacccgaaga gcagattgat caaaaaattt accgcactag
    27121 gcccgtatat tcgtgaaggt aagtgcgaag ataatcgatt ctttttcgat tgtctggctg
    27181 tatgcgtcaa cgtgaaaccg gcaccggaag tgcgtgaatt ctggggctgg tggatggagc
    27241 tggaagcgca ggaatcccgt tttacctaca gctaccagtt tggtctgttc gataaagcag
    27301 gcgactggaa gagtgttccg gtaaaagata ctgaagtggt tgaacgactg gagcacaccc
    27361 tgcgtgagtt tcacgagaag ctgcgtgaac tgctgacgac gctgaatctg aagctggaac
    27421 cggcgaatga ttttcgtgat gaaccggtga agttaacggc gtgagtgaaa tgtgccggat
    27481 gcatcacatc cggcaatatt cattaaaact gatacgtcat cccaaccgcg acagtatcat
    27541 cattattaat attcagctta ttatcgctat ccagttggtt gattttataa tcaacaaacg
    27601 ctgacatatt tttgttgaaa taatacgtag caccgacgtc gatataattg accagatctt
    27661 catcaccgat gccctcaata tctttccctt tcgataagac ataacccagc gatggacgca
    27721 gaccaaagtc aaactggtat tgagcgaccg cttcaaagtt ctgtgtctta ttggcaaagc
    27781 cgccagttat tggcgtcatt ttgcgtgttt cagaatagaa agttgccaga taaatattat
    27841 tggcatcgta tttcagacct gttgcccatg cttctgcacg cttgcctgtg ccacggcttt
    27901 gcaggttctg ctcgttggtg cgatctgagt tggtataggc cccactaatg gcgaaatcgc
    27961 tgccgccaaa gtcatatgtc aatgacgtgc cgaagccatc gccgttttgc tttttaacgt
    28021 cgcggttttc gtttttccct tgatattgca gggttaagtt caggccatcg ataacgccga
    28081 agaagtcggt gttccgatac gtcgccagac cgctggcgcg tttggtcata aagttgtcgg
    28141 tctgcgccga ggaatcgcca ccaaattccg ggaacatatc ggtccaggct tccacgtcat
    28201 acaacgcgcc caggttacga ccataatcga aagaacccaa atctttatat ttcaacccgg
    28261 caaaagcgag acgcgttttt tgctgtgcag tatcactctc cgctttattt ccggcaaact
    28321 ccgcttccca ccgaccataa ccagtcagtt gatcgttaat ttgagtttca cctttaaaac
    28381 caaaacggat ataactctgg tcgccatctt tactggcgtt atcactcata taatgcatgg
    28441 ctttaacttt gccatagaca tccagtttat taccgtcttt attatatatt tctgcagcct
    28501 gtacagatgc agatgccaca atgcccatca ccactaatgc cagagtgctc tttttcattt
    28561 tcattcctga ttttaattaa cgcgcgaata ttcagcggga gagccccgtt gaaaacagga
    28621 aagtttttaa cctgagattg ttaaagatat attacagatt aatgatatt
//
LOCUS       DAJTSF010000048        28419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000048 DAJTSF010000000
VERSION     DAJTSF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.048"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            563..1333
                     /locus_tag="OO335_002193"
     CDS             563..1333
                     /locus_tag="OO335_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6002567.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1324..1797
                     /locus_tag="OO335_002194"
     CDS             1324..1797
                     /locus_tag="OO335_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6002568.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1904..2443
                     /gene="dnaT"
                     /locus_tag="OO335_002195"
     CDS             1904..2443
                     /gene="dnaT"
                     /locus_tag="OO335_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6002569.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2446..3183
                     /gene="dnaC"
                     /locus_tag="OO335_002196"
     CDS             2446..3183
                     /gene="dnaC"
                     /locus_tag="OO335_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6002570.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3232..3726
                     /locus_tag="OO335_002197"
     CDS             3232..3726
                     /locus_tag="OO335_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6002571.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3966..6257
                     /gene="opgB"
                     /locus_tag="OO335_002198"
     CDS             3966..6257
                     /gene="opgB"
                     /locus_tag="OO335_002198"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6002572.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6395..7417)
                     /gene="lgoD"
                     /locus_tag="OO335_002199"
                     /pseudo
     CDS             complement(6395..7417)
                     /gene="lgoD"
                     /locus_tag="OO335_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7556..8470
                     /locus_tag="OO335_002200"
     CDS             7556..8470
                     /locus_tag="OO335_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6002573.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8685..10046
                     /locus_tag="OO335_002201"
     CDS             8685..10046
                     /locus_tag="OO335_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6002574.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="OO335_002202"
     CDS             complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="OO335_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR6002575.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="OO335_002203"
     CDS             complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="OO335_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6002576.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12596..13885
                     /locus_tag="OO335_002204"
     CDS             12596..13885
                     /locus_tag="OO335_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6002577.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13882..15348
                     /gene="hpaE"
                     /locus_tag="OO335_002205"
     CDS             13882..15348
                     /gene="hpaE"
                     /locus_tag="OO335_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6002578.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15350..16201
                     /gene="hpaD"
                     /locus_tag="OO335_002206"
     CDS             15350..16201
                     /gene="hpaD"
                     /locus_tag="OO335_002206"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6002579.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16211..16591
                     /locus_tag="OO335_002207"
     CDS             16211..16591
                     /locus_tag="OO335_002207"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6002580.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16659..17462
                     /gene="hpaH"
                     /locus_tag="OO335_002208"
     CDS             16659..17462
                     /gene="hpaH"
                     /locus_tag="OO335_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6002581.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17473..18276
                     /gene="hpaI"
                     /locus_tag="OO335_002209"
     CDS             17473..18276
                     /gene="hpaI"
                     /locus_tag="OO335_002209"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6002582.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLPQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18298..19653
                     /gene="hpaX"
                     /locus_tag="OO335_002210"
     CDS             18298..19653
                     /gene="hpaX"
                     /locus_tag="OO335_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6002583.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19663..20553
                     /gene="hpaA"
                     /locus_tag="OO335_002211"
     CDS             19663..20553
                     /gene="hpaA"
                     /locus_tag="OO335_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR6002584.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20580..21517
                     /locus_tag="OO335_002212"
                     /pseudo
     CDS             20580..21517
                     /locus_tag="OO335_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21744..23302
                     /gene="hpaB"
                     /locus_tag="OO335_002213"
                     /pseudo
     CDS             21744..23302
                     /gene="hpaB"
                     /locus_tag="OO335_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23320..23832
                     /locus_tag="OO335_002214"
     CDS             23320..23832
                     /locus_tag="OO335_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6002585.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24225..26375
                     /gene="btsT"
                     /locus_tag="OO335_002215"
     CDS             24225..26375
                     /gene="btsT"
                     /locus_tag="OO335_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6002586.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26425..26628
                     /locus_tag="OO335_002216"
     CDS             26425..26628
                     /locus_tag="OO335_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6002587.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26639..27595
                     /gene="yjiA"
                     /locus_tag="OO335_002217"
     CDS             26639..27595
                     /gene="yjiA"
                     /locus_tag="OO335_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6002588.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27664..>28419)
                     /locus_tag="OO335_002218"
     CDS             complement(27664..>28419)
                     /locus_tag="OO335_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCR6002589.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6953 a         7427 c         7128 g         6911 t
ORIGIN      
        1 ttaaatgttt ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc
       61 atatcagcat actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa
      121 atgataacct ggtgaaatag cctggagaca tttaaaatat ctacattaag ttgttagaaa
      181 tacttaatga aaaacagaaa tctcattcat ttaatttata attaaatgat atctcttccc
      241 gcagaatgcg ggaatcacac aatcaatatt tattcaataa aatctgatga ttttaacttg
      301 ttaacactaa aaaacgcgcc cagctatcta ttatcctgac ccgtaaaggc taaacaataa
      361 attcattata gtgataatat tcatgtgata gtttgtttgc ggcgagagat aattcgcttt
      421 ttatcaccga gcttgtccca ataaccagga ttaatcagcg caattttgat cttaacctac
      481 cgcaaaacaa ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca
      541 cgtctatgac aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cgcggctttg
      601 tatccagtgt ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct
      661 ttcctcacga ctgggtcggg cgctgggaat ggacagcgtc gaaagttcta tctcttcgaa
      721 cgccatagtg ctgacaacta ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca
      781 cgatcgcggc attaatatgc atgtggtgac tgaagtccag cacattgtga ttcttgcgga
      841 gcatcatctg ctggattaca aaggcgtaga gaaacgattt agccaaattc aaccattacg
      901 ttacccaaga tggctggtag ccttaatggt tggcctttct tgcgcctgtt tctgtaaact
      961 caataacggt ggctgggatg gtgccgtcat caccttcttt gccagtacga ccgcgatgta
     1021 tatccgccag ctgctggcac aacgtcatct tcatccacag atcaactttt gccttaccgc
     1081 tttcgccgcc accaccattt ccggattgct tttgcaactc cccactttca gcaatacccc
     1141 caccattgcg atggccgcca gcgttctgct gctagtgccg ggctttccgt tgattaatgc
     1201 cgtcgccgat atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag
     1261 tctgctgaca ctggctacct gcgtcggcgt agtgatggca ctgacgattt gggggctacg
     1321 cggatgggtg tgatcgaatt tctgttagcg ttggcgcagg atatgatcct cgccgccatt
     1381 cctgcggtcg gctttgcgat ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg
     1441 ctgcttggct cgataggtca tggttcccga atgatcttga tgaccagcgg gttgaatatt
     1501 gagtggtcaa cctttatggc ttctatgctg gtcggtacca ttggtattca atggtcgcgc
     1561 tggtatctgg cgcatccgaa agtgtttacc gtggcggccg ttatccctat gttcccgggc
     1621 atatcggctt ataccgcaat gatttcgtcg gtaaaaatca gccagttagg ttacagcgaa
     1681 ccgttgatga ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcgtta
     1741 tccatcggtc tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat
     1801 tgcctcgctt aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa
     1861 gttatctccc ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt
     1921 gaccccggac gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa
     1981 agctgaaggc ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac
     2041 gcctgcacgc ctggctgaac tgctggcgct ggaagaaaag ctagcgcgtc cgggaagcga
     2101 tgtcgctctg gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtacccat
     2161 ggggaaattc gccatgtatc cggactggca acccgatgcc gattttatcc gcctggcggc
     2221 gctatggggc gtggcgctaa gagagccggt gaccaccgaa gaactggcct cattcattgc
     2281 ctactggcag gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg
     2341 cagcctgcaa atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt
     2401 cagtgaacct gacagccaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc
     2461 gacctgatgc aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg
     2521 ggtgaagagc tactggcctg gcagaaagaa caaggggcga tccgctccgc cgctctcgaa
     2581 cgtgaaaatc gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcgtccactg
     2641 catcagaact gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta
     2701 agcaaagcgc gccagtatgt cgaagagttt gacggcaaca tcgccagctt catcttttct
     2761 ggtaagccag gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta
     2821 cgcggtaaat ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc
     2881 ttcaggaata gcggtaccag cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg
     2941 ctggtgatcg atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac
     3001 cagatcgtcg atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat
     3061 atggaagaga tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac
     3121 agtttatggg tgatcttcaa ctgggatagc taccgtagcc gggtaacggg taaagagtat
     3181 taatccccgt ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa
     3241 actattaaac atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg
     3301 catgctgcgt cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac
     3361 agcacgacac aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcgga
     3421 aaccaagcct taagcgcaga taacatgaac aatgccgcag gcattctgca atactgcgcg
     3481 aagcaaaagc tggcttcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag
     3541 ctgggcctga acagtgaaga gcaaaaagaa gacaccaact atctggacgg tattcagggt
     3601 ttgctgaaaa caaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg
     3661 gcagaaaagg tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt
     3721 tcctgaaccg cgaaatgtta cccggctgac gcggctctct cctgcccttt tccctgctcc
     3781 tggacggttt tccccctgtt acacgggaag cgaccgcgat caaaaaagtc tgtttataaa
     3841 ctaattccta acaacatcgc atttttttga cactagaatt gcagcaatat ggcgacgcca
     3901 ttacattgtg cttctcaaaa agcttaaacg ttggctaaac ggcccggttt agtgaggatc
     3961 gcccattgtc agaactactc tctttcgccc tttttctcgc ctctgtgctg atttacgcat
     4021 ggaaagcggg acgtaacacc tggtggtttg cagccacgtt aacggtgctg gggctatttg
     4081 tcgttttaaa tatcaccctg tttgccagcg actattttac tggcgatggt attaacgacg
     4141 cggttctcta taccttaacc aacagcctga ccggtgctgg cgtcagcaaa tacatcctgc
     4201 cgggtatcgg cattgtgctg gggctgacgg cggtgttcgg tgcgctgggc tggatcctgc
     4261 gccgtcgtcg tcatcatccg caccattttg gttacagcct gctggcgctc ttactggcgc
     4321 tgggttcggt ggacgccagc ccggcatttc gtcagataac ggaactggtg aaatcccagt
     4381 cacgcgatgg cgacccggac tttgcggctt attataaaga gccgtcgaaa actatccctg
     4441 acccgaaact caacctggtt tatatctacg gcgaaagtct cgagcggacc tattttgata
     4501 acgaagcttt cccggatctc acgcctgaac tgggcgcgtt gaaaaatgaa ggcctggatt
     4561 tcagccacac gcagcagctg ccaggaacgg attacacgat tgcgggcatg gtggcttctc
     4621 agtgcggcat accgctgttc gccccctttg aaggcaacgc ctccgcctct gtctccagct
     4681 tcttcccgca gaacatctgt ctgggcgata tcctgaaaaa ctcgggttat cagaactatt
     4741 tcgtgcaggg cgcaaatctg cgttttgccg gtaaagatgt gttcctgaaa tcgcacggct
     4801 tcgaccactt atacggctca gaagagctga aaagcgtggt agccgacccg cactatcgca
     4861 acgactgggg attctacgac gataccgttc tcgatgaagc gtggaaaaag tttgaagagc
     4921 tttcccgctc aggtcagcga ttctcactgt ttaccctgac agtcgatacc catcacccgg
     4981 atggttttat ctcgcgcacc tgtaaccgca aaaaatatga ttttgacggt aagccgaatc
     5041 agtcattcag cgcggtaagt tgcagccagg agaatatcgc gacgtttatc aacaaaatca
     5101 aagcgtcacc gtggtttaaa gatactgtca tcgtcgtctc ttctgaccat ttagcgatga
     5161 acaacacggc gtggaaatac ctcaataagc aggatcgcaa taacctgttt tttgtcattc
     5221 gtggcgacaa gccgcagcaa aagacgctgg cagtgaagcg taacacgatg gataacggcg
     5281 cgacggtgct ggacattctc ggtggcgata actatctcgg acttggtcgt agcagtttat
     5341 ccgggcagtc gatgtcggaa atcttcctca atatcaaaga gaaaacattg gcgtggaagc
     5401 cagatatcat ccgcctgtgg aaattcccta aagagatgaa agagttcacc atcgaccagc
     5461 agaaaaacat gattgccttc tcgggtagcc atttccgttt gccgctgctg ttgcgggttt
     5521 cagacaaacg cgtggaaccg ctgccggaaa gcgaatactc agcaccgctg cgtttccagc
     5581 tggccgattt cgctccacgc gacaatttcg tctgggttga cagttgctac aagatggcac
     5641 aactctgggc tccggaactg gcactttcca ccgactggtg tgtctcgcag gggcagcttg
     5701 gcggtcagca aattgttcag catgttgaca aaacaacatg gaagagcaag acggcattta
     5761 aagatacggt catcgacatg gcgcgttaca aaggcaatgt cgatacgctg aagattgttg
     5821 ataacgatat tcgctacaaa gccgacagtt tcatctttaa cgtcgccggt gcgccagaag
     5881 aggtgaaaca gtttagcggg atttcccgtc cggagtcgtg gggccgctgg tccaacgcgc
     5941 agctgggcga tgaagtaaaa atcgagtaca agcatccgct gccgaagaaa tttgacctgg
     6001 tgattaccgc caaagcatac ggcaataacg ccagccgtcc tattccggta cgcgtaggca
     6061 atgaagaaca aactcttgtg ctgggcaatg aagtgaccac caccacactg catttcgata
     6121 acccaaccga tgccgacaca ctggtaattg tgccgccgga acctgtctca accaacgaag
     6181 ggaatatcct cggacactcg ccgcgtaagc tcgggatcgg catggtggaa attaaagtgg
     6241 tagaacgtga agggtaattc ctgactgatg tgtaggtcgc atagggtgcg acctacacgc
     6301 cttctccggc ttacaacaca ttattaagaa acaaaaaaaa cagcgcccaa cacttccccg
     6361 gacgctgcat ggcaacttta ataatctcgc cttatcagaa agtaattaca cccttaatca
     6421 actcacgatt gttaatcaca tcgcgctcgt aggtttctgc cagcgtggcg aacggatagc
     6481 gatgggttaa catcatgtcg gcagtgattt tcccttccgc catcagtcga ccgactttag
     6541 cgaaatcttc cggcgtggcg ttgcggctgc ccatcatcgt cgtttctttt ttatggaatt
     6601 ccggatcgga gaactgcaac tcacctttaa acagaccgac aaataccacc gtgccgccgt
     6661 gacgaatcag attcacggta ttattcatcg catgttgatt acctgtcgcg tcgatcactt
     6721 tctgcgccag cgaaccacca aactgcgccc gcagctgcgc gtcaaaatct tcggctgacg
     6781 ggtccagcac gggcaactcc agacgcgttg ccacatgttc acggcgcgcc ggactggtat
     6841 ccgccaccac cacctgtgcg ccatcggctt tagcgattgc cgccgcgccc agaccgattg
     6901 gccccgcccc gaccaccagc acctgctcgc cgggagcaat ggctgcgcga cgcaccgcat
     6961 gagcgctaat agcgaaaggt tcaatcaatg cggccgcctg cgggtcaata ccgtctgcgg
     7021 gcaaaatgtt cgccaccggc acgctcaaat actcactaaa accgccatcc tgatgcacgc
     7081 caatgactga aattttttca cagcagttgg tacgcccgct tttacacgcc gggcattgct
     7141 gacaggcaac ataagggatc acagcaactt gctgaccatt tttaagatca gcaatatttt
     7201 tacccagccc aacaatctcc ccacatattt catggcctaa aacccgtgga taactaaaaa
     7261 atggttgatt tccaccctag gcatgaatat cggtaccgca aatcccgaca gacttaattt
     7321 ttattaatgc ttcattgtca cccggaatag gtatctcgcg ttgtttccag actaattctt
     7381 tcggctgctg gcaaattaaa acattcatcg tagacatgat tcggctatcc attttttgtt
     7441 gccttagata tcaatgaaaa tgaaaaatgg cagcggaacg gaaaatctgt tttgtgaaaa
     7501 cacacgcata aaaatgtttt aaatcgggtt ttaatggact cacaaggagg aaaatatgag
     7561 tcgttcacaa aatttacgcc acaatgtgat taaccaggtc attgatgata tggcgcgggg
     7621 tcatatccct tccccacttc catcgcaaag tgcgctggcg gaaatgtaca acatcagccg
     7681 cactacagtg cgtcatattc tcagccaatt acgcgaatgc ggcgtcttaa cgcaggttgg
     7741 aaacgactat gttattgcgc gtaaacctga ccatgatgac ggtttcgcct gtactaccgc
     7801 ttcaatgagt gaacaaaaca aagtgtttga gcaggctttt ttcacgatga taaaccagcg
     7861 tcagttacgc cctagggaaa ctttttctga actgcaactg gctcgggccg cgggtgtcag
     7921 cccggtggtt gtaagagaat atcttttaaa attcggccgt tataatctta ttcaaagcga
     7981 aaagcgcggc cagtggagca tgaagcaatt cgatcaatcc tacgccgagc agctgtttga
     8041 gctacgcgaa atgctcgaaa cacattcact tcagcacttt ctcaacctgc ctgatcatga
     8101 tccccgctgg ctgcaggcga aaaccatgct agaacgtcat cggctactgc gtgacaacat
     8161 cggcaatagc tttcgcatgt tctcgcaact tgacagagat tttcactcct tactgctttc
     8221 agctgccgac aatatctttt ttgatcaatc gcttgagatc atctcagtca tctttcattt
     8281 tcactatcaa tgggacgaaa gcgatcttaa acagcgcaat atcattgccg tcgatgaaca
     8341 tatgaccatt ctcagcgctc tgatttgccg cagtgacctt gatgccaccc ttgcgctccg
     8401 taaccatttg aattcagcta aacaatcaat gattcgatcc attaatgaga acacgcgtta
     8461 tgctcattaa gtacggatta aaaacagcaa actattagcg ggtgcacggt tttatcaaaa
     8521 gataaatacc gggaaattcc cgcttaccta tgctcacaat cagtcgacta aaaatgtttt
     8581 tgtgagcggt agtaaagtcc taaaacttta acctgccgtt aatgtcaacc ttgcgtaatg
     8641 cacaattacg taaacaatta aaattataaa tacaggagtc cggcgtggaa aaagaaaata
     8701 tcaccctcga tccgcgttct tcatttactc catcttcgtc ggcagatatt cccgtgccac
     8761 cagatggatt agttcaacgc agtacccgaa ttaaacgcat tcaaaccacc gccatgttgt
     8821 tattattttt tgcggcggta atcaattatc tcgaccgcag ttcgctgtcg gtagcaaatt
     8881 taacgattcg tgaagaattg ggattaagtg ccaccgaaat cggcgctttg ctctccgtgt
     8941 tttcactcgc ttacggcatt gcgcaacttc cttgcggccc actattggat cgtaaaggcc
     9001 cacgcctgat gctgggactg gggatgttct tctggtcact gttccaggcc atgtctggca
     9061 tggtgcacag ctttacgcag ttcgtgttgg tgcgtatcgg tatggggatt ggtgaagcgc
     9121 cgatgaaccc atgcggtgta aaagtcatta acgactggtt caacatcaaa gagcgcggac
     9181 gcccgatggg cttcttcaac gcagcttcta ccattggcgt tgctgtaagc ccaccgattc
     9241 tggcggcaat gatgctggtg atgggctggc gcgggatgtt tattaccatt ggtgtactgg
     9301 ggatttttct cgccatcggc tggtatatgc tctatcgcaa ccgcgagcac gtagaactga
     9361 ctgccgttga acaagcttat ctcaatgcag gtagcgtcaa tgcccgccga gatccgctca
     9421 attttgccga atggcgcagc ctgttccgta accgcacaat gtggggaatg atgctcggat
     9481 tcagtggcat caactacact gcgtggctgt atctggcctg gcttcctggt tacctgcaaa
     9541 cagcctataa cctggattta aaaagcacag ggttgatggc ggctatccct ttcctgtttg
     9601 gggctgccgg gatgctggtc aacggttacg ttactgactg gctggtcaaa gggggaatgg
     9661 ctccgattaa aagccgtaag atctgcatta ttaccgggat gttctgttct gccgccttta
     9721 cgctgatagt accacaagcg acaacatcca tgatggcggt tctgctgatt ggcatggcac
     9781 tgttctgtat tcactttgcc ggaacatcct gctggggctt gatccacgtc gcagttgctt
     9841 ctcgcatgac tgcgtcggtg ggcagtatcc agaactttgc cagcttcatc tgcgcctctt
     9901 tcgcgccgat cattactggt tttattgttg atacaaccca ctcattccgt ctggcactaa
     9961 tcatttgcgg ttgcgtcacc gcagcgggag cactggcgta catcttcctg gttcgccagc
    10021 cgatcaacga cccgcgtaaa gattaaactc cctatgcccg ataacatttt gcttatcggg
    10081 catttttatg gcgattaaaa cgtttcccag ttctccccgc tatctgccac tgccatttta
    10141 cgcggcgtag ctggcgtcac ggtttttacc acagccgatg tttcacgctg ctgttgctgc
    10201 tgttgctgaa tccggaacac tgccacagct tcggtcaggc gactggcctg ctcttccagc
    10261 gcggcggcgg cagcggcaga ttcttccacc agcgcggcgt tctgttgagt tacccggtcc
    10321 atctcagcaa ccgctaagcc aacctgatcg ataccacggc tctgctcatc agaagcagaa
    10381 gctatttcgc ccataatgtc cgtcacgcgg gtcacagcac tgacaatctc cgccattgtt
    10441 tccccggcgc tttcgaccag cgtagagcca acatccactt tccccaccga gtcttcaatc
    10501 aggcttttaa tttcacgaac cgcctgggcg ctacgctggg ccagattacg cacttctccc
    10561 gcaaccaccg caaaaccacg tccttgctca cccgcacgcg ctgcttcaac cgccgcgttc
    10621 aaagccagaa tattggtctg gaaggtaatg ccgtcaatta cgctgataat atcggcgatt
    10681 ttctgcgaac tggtggagat atcgcgcata gtctgcacca cgttatctac cactttgccg
    10741 ccgcgttgcg ccgtttcaga agcacttaat gccagatggc tggcctggcg cgcattctcg
    10801 gcgttctgtt tcaccgttgc ggtcagttgc tccatgctgg ctgccgtctc ttccagcgaa
    10861 gcggcctgtt gctcggtgcg cgaagagaga tcattattgc cggtagcgat ttcgctggca
    10921 ccgctataga tggcattggc cccgttgcgc acatcaccga cggtacgcat cagctctccc
    10981 tgcatatggc gcaaactctc tgccagttgc cccatctcat tagagccatc cacctcaatc
    11041 ggtttcacca gatcgccgcc tgcaatatga cgaatgctgt caatcaggcg attcattggc
    11101 gctaccagcg aggctttaat accgaaccag acggcgaaga tgaccgccag tacgacgatc
    11161 atcacgccca ccagaatcca catcgcctgg ctgtaggagg cattgttatc gctgacggcg
    11221 atatcataga gccgatcgtt ttgctccatg taagccacat actgcttctc gaaaccgtcc
    11281 tgatatccct gggtcggctg atcaaagaac tcgttgattt tgcctgcacc taacagttgg
    11341 atcagctccg ccagcgcatt gtgataaata tcgtaattac gtttgatctc tgccgctgcg
    11401 gcggtgctct gacgcgggtc acgcggcaac gcttcgtaat ccgcccagtt tttttccgcc
    11461 tgtttcagcg aaatactggc actctgcatc agctcagcaa cggttgaacc gctaccaata
    11521 ttattctgat ccatcatgta gcggataccc gcgcggttga gggtgttacg cgtctgcaac
    11581 aacgcgaccc agctgccatt cagcgtgggt tgctgctggc gaatggtttg taaaacagtg
    11641 aaattttctt tgtcattctt taaggcatta aaaaacagac cgcctgatgt cagttgtaaa
    11701 aggccaaaaa cggccaaaac cagcagtaag ctggtcacga tcttgatacg ttttaacatg
    11761 gtttctcttt cctgtggacc gatgcaagat tttcggcctg gaaagcaaaa actttatgaa
    11821 aaggtgtaac ccgccatcca tggaaaaacc aggcgctggt tatcagatac taaaaagtta
    11881 ttcattatcg cccgggatgt cttcttgtcg ggaattgccc agagcaataa attcctctaa
    11941 cagatgggtt aactgttgca ttttctcggc agtaaactga gcttcaatct gccgataggc
    12001 ttcttcaatc tgtgtctgag cgcggttata cagcgcctgc ccctctttgg tcagcgagat
    12061 atagagcttc cgctgatcat tgatcggctt taatcgcaac actaaaccgt cgcgttccat
    12121 ccgcgtgagg atcccggtca ggcttgggcg caaaatgcag gcgcgatacg ccagatcgtg
    12181 aaaatccatt gatgggcttt ccgccagaat acgcacgatg cgccactgtt gctcagtcaa
    12241 attatgccgt ttaacgattg ggcgaaaata actcatcgcc gcttctcgcg cctggagcaa
    12301 cgcaatggtt agtgagtcgt gcattatctt tcccctgaaa aattcaaaat cattaatata
    12361 gaaacaatta atcatcacgt taccgggact gagctgcccc cgcaattctt cattttatca
    12421 aaattgatga tattttatta acttactgat atttaatatt ttattatctt taaatgtaaa
    12481 tagtttgtta attagatcac atttatatca cttacttttg ccaaactacg caatcgggaa
    12541 taaaagtaat cattaacata ttaatgatta agatgatcga atccgaggag tggtaatgaa
    12601 aggcactatc ttcgccgtag cgttgaacca tcgcagccaa cttgatgcct ggcaggaagc
    12661 gttccagcaa tctccctaca aagccccgcc taaaactgcg gtctggttta ttaaaccgcg
    12721 caatacggtg attggttgcg gtgaaccgat tccctttcca cagggtgaaa aagtactgag
    12781 cggtgcgact gttgcgctga ttgtggggaa aacggcgacg aaagtacgtg aagaagatgc
    12841 ggcagagtac atcgccggat atgcgctgga taacgacgtc agcctgccgg aagagagctt
    12901 ttaccgtccg gcaatcaaag caaaatgtcg tgatggattc tgccccattg gcgaaaccgt
    12961 ggctctcagc aatgtcgata atctgactat ctataccgag atcaacgggc gtcctgccga
    13021 tcactggaat accgccgatt tacaacgtaa cgccgcacag ctgctgagcg ccctgagcga
    13081 atttgccacg ctgaatccag gcgatgcgat tctgctcggc acgccacagg cgcgcgtgga
    13141 aatacagcca agcgatcgcg tgcgtgttct cgcagaaggt ttcccgccgc tggaaaatcc
    13201 ggtagtggac gaacgtgaag tgaccacgcg caagagcttc ccaacgctgc cacacccgca
    13261 cggtacgctg tttgccctcg gcctgaacta cgccgaccac gccagcgaac tggaatttaa
    13321 gccaccggaa gagccgctgg tgttcctgaa agcgccgaat accctcactg gcgacaacca
    13381 gacctccgtg cgcccgaaca atattgaata catgcactac gaagcggagc tggtggtagt
    13441 tattggcaag caggcgcgta acgtcagcga agccgatgcc atggattatg tcgcgggcta
    13501 caccgtgtgt aacgactacg ccattcgcga ctatctggaa aactactacc gccctaacct
    13561 gcgggtcaaa agccgcgacg gactgacgcc gatgctttca accatcgtgc cgaaagaggc
    13621 gatcccggac ccgcataatc tgacccttcg caccttcgtc aacggcgagt tacgccagca
    13681 aggcaccacc gccgatctga tcttcagcgt gcccttcctg atcgcctact taagcgaatt
    13741 tatgaccctg aatccgggcg acatgatcgc caccggcaca ccaaaaggct tatctgacgt
    13801 ggtgcctggc gatgaagtag tggtggaagt agaaggcgtg ggccgcctgg tgaaccgaat
    13861 tgtgagtgag gaaacagcga aatgaaaaaa gtaaatcatt ggatcaacgg caaaaatgtt
    13921 gcaggtaacg actacttcca gactaccaat ccggcaacgg gtgaagtgct ggcggatgtg
    13981 gcctctggcg gtgaagcgga gatcaatcag gcggtagcgg cagcgaaaga ggcgttcccg
    14041 aaatgggctg atctgccgat gaaagagcgt gcgcgcctga tgcgccgtct gggcgatctg
    14101 atcgaccaga acgtgcctga gatcgccgcg atggaaaccg cggacacggg cctgccgatc
    14161 catcagacca aaaatgtgtt gatcccacgc gcttctcaca actttgaatt tttcgcggaa
    14221 gtctgccagc agatgaacgg caagacctat ccggttgacg acaagatgct caactacacg
    14281 ctggtgcaac cggtaggcgt ttgtgcgctg gcgtcgccgt ggaacgtgcc gtttatgacc
    14341 gccacctgga aggtcgcgcc gtgtctggcg ctgggcaata ccgcggtgct gaagatgtcg
    14401 gaactctccc cgctgaccgc cgaccgcctg ggtgagctgg cgctggaagc tggtattccg
    14461 gcgggcgtgc taaacgtggt acagggctac ggcgcaaccg caggcgatgc gctggtccgt
    14521 catcatgacg tgcgtgccgt gtcgttcacc ggcggtaccg ccaccgggcg caacatcatg
    14581 aaaaacgccg ggctgaaaaa atactccatg gagctgggcg gtaaatcacc ggtgctgatt
    14641 tttgaagatg ccgatattga gcgtgcgctg gacgccgccc tgttcaccat cttctcaatc
    14701 aacggcgaac gctgcaccgc cggttcgcgc atctttattc agcagagcat ctatccagaa
    14761 ttcgttaaac gctttgccga acgcgccaac cgtctgcgcg tgggcgatcc gaacgatccg
    14821 aatacccagg ttagggcgct tatcagtcag caacactggg aaaaagtctc cggctatatc
    14881 cgtctcggca ttgaagaagg cgcaaccctg ttggcgggcg gcccggataa accgtccgac
    14941 ctgcctgcgc acctgaaagg cggcaacttc ctgcgcccaa cggtgctggc ggacgtagat
    15001 aaccgtatgc gcgttgccca ggaagagatt ttcgggccgg tcgcctgcct gctgccgttt
    15061 aaagacgaag ccgaaggctt acgcctggca aacgacgtgg agtacggcct cgcgtcgtac
    15121 atctggacac aggatgtcag caaagtgtta cgcctggcgc gtggcattga agcaggcatg
    15181 gtgttcgtca acacccagaa cgtgcgcgac ctgcgccagc catttggcgg cgtaaaagcc
    15241 tccggcaccg ggcgtgaagg cggtgagtac agcttcgaag tgttcgcgga aatgaagaac
    15301 gtctgcattt ccatgggcga ccatccaatt ccgaaatggg gagtctgata tgggtaagtt
    15361 agcgttagcc gccaagatca cgcacgtacc gtcgatgtat ctctctgaac taccaggaaa
    15421 aaaccacggt tgtcgccagg gtgcgatcga cgggcataaa gagatcagca agcgttgccg
    15481 ggaaatgggc gtcgatacca ttatcgtttt cgatactcac tggctggtca acagtgctta
    15541 tcacatcaac tgtgcagacc attttgaagg cgtctacacc agcaacgagc tgccgcattt
    15601 tattcgcgac atgacctaca actacgaggg caacccggag ttggggcagc taattgccga
    15661 ggaagcctta aagctcggcg tgcgggcaaa agcgcacaat attcccagcc tgaaactgga
    15721 atacggcacg ctggtaccga tgcgctacat gaatgaagac aagcacttca aagtggtctc
    15781 catttcggct ttctgcacgg ttcacgattt tgccgacagc cgcaagctgg gcgaagcgat
    15841 tctgaaagcg atcgaacagt acgacggcac cgtggcggtc cttgccagcg gttcgttatc
    15901 gcaccgcttt attgacgatc agcgtgcgga agaagggatg aacagctaca cccgcgagtt
    15961 cgaccgccag atggacgagc gtgtggtgaa gctgtggcgc gaaggccagt tcaaagagtt
    16021 ttgcaatatg ctgccggagt acgccgacta ctgctacggc gaaggcaata tgcacgacac
    16081 ggtgatgctg ctggggatgc tcggctggga taaatacgac ggcaaggtgg agtttattac
    16141 cgagctgttc ccaagctctg gcaccggtca ggttaacgct gttttcccgc tgcccgcgta
    16201 aggacgtttt atgccgcact ttatcgtgga atgtactgaa aatattcgtg aagaagcccg
    16261 cttacctgag ctgtttgcca gtgttaacac tgcactggcg gcaacgggga tttttccgct
    16321 tgggggaata cgtagccggg cgcactggat cgatacctgg caaatggctg atggcaagca
    16381 cgattacgcc tttgtgcata tgacgctaaa aatcggcagc ggacgcagtc tggaaagccg
    16441 tcaggaagtg ggcgaaatgc tgtttgatct gattaaaacg cacttcgcct cactgatgga
    16501 gagccgttac ctagcgcttt catttgagat tgcagagctg cacccgacgt tgaatttcaa
    16561 acagaacaac gtacacgcgt tgtttaaata aatttgcacc agcttccagg ccggatgata
    16621 aggcgctgcc gcatccggca atgccacagg atatcgctat gttcgataaa cacacccaca
    16681 ccctgatcgc ccagcgtctg gatcaggcag aaaaacagcg cgaacagatc cgcgcgatct
    16741 cgctggatta cccggagatc accatcgaag acgcctacgc cgtgcagcgc gaatgggttc
    16801 gcctgaaaat cgccgaaagt cgcacgctga aaggccacaa aatcggtctg acctcgaaag
    16861 cgatgcaggc aagctcgcag atcagcgaac cggattacgg tgcactgctg gacgacatgt
    16921 tcttccacga tggcagcgat atcccgaccg atcgctttat cgtgccgcgc attgaagtgg
    16981 agctggcttt tgtgctggca aaaccgctgc gtgggccaaa ctgcacgctg ttcgacgttt
    17041 acaacgccac ggactatgtg atcccggcgc tggagctgat cgacgctcgc tgccacaaca
    17101 tcgatccgga aacccagcgc ccgcgcaaag tattcgacac catttccgat aacgccgcca
    17161 acgccggagt gatcctcgga ggtcgtccga tcaaaccaga cgaactggat ctgcgttgga
    17221 tctccgccct gatgtatcgc aatggcgtga ttgaagaaac cggcgtcgcc gctggtgtgc
    17281 tgaatcatcc ggcaaacggc gtggcctggc tggcgaacaa actcgcaccg tatgacgtac
    17341 aactggaaac cgggcaaatc attctcggtg gttcgttcac cctcccggtt ccggcgcgta
    17401 agggcgacac cttccacgtc gattacggca acatgggctc cattagctgc cgctttgttt
    17461 aaggagagaa cgatggaaaa cagttttaaa gcggcgctga aagcaggccg tccgcagatt
    17521 ggattatggc tggggctgag cagcagctac agcgcggagt tactggccgg accaggattc
    17581 gactggttat tgatcgacgg tgagcacgcg ccgaataacg tgcaaaccgt gctcccccaa
    17641 ctacaggcga ttgcgcccta ccccagccag ccagtggtac gcccatcgtg gaacgatccg
    17701 gtgcaaatca aacaactgct ggacgtaggc acacaaacct tactggtgcc gatggtacaa
    17761 aacgccgacg aagcccgtaa agcggtacgc gccacccgtt atccccccgc cggtattcgc
    17821 ggtgtgggca gtgcgctggc tcgcgcctcg cgctggaatc gcattcctga ttacctgcaa
    17881 aaagccaacg atcaaatgtg cgtgctggtg cagatcgaaa cgcgtgaggc aatgaagaac
    17941 ttaccgcaga ttctggacgt ggaaggggtc gacggcgtgt ttatcggccc ggcggatctg
    18001 agcgccgata tgggttatgc cggtaatccg cagcacccgg aagtacaggc cgccattgag
    18061 caggcgatcg tgcagatccg cgaagcgggc aaagcgccgg ggatcctgat cgccaatgag
    18121 caactggcaa aacgctatct ggaactgggc gcgctgtttg tcgccgtcgg cgttgacacc
    18181 accctgctcg cccgcgccgc cgaagcgctg gcagcacggt ttggtaatag ttcttctatt
    18241 tcgatggcga agcaaaacaa caactctgtc tactaaacgt acgatgtgga gcgcatcatg
    18301 acgacctcta ccctgcaata taaagataat aaaactgttg aaattgaaca tcgtgtcatt
    18361 aataaattat ttcgtcgcct aatcattttt ctttttattc tgtttgtttt ttcgtttctt
    18421 gatcgcatca atataggatt tgccggactg acaatgggca aggatcttgg cctgacgtcc
    18481 acaatgtttg gcctcgccgc aacactcttt tatgtcacgt acgtcatgtg cgggatcccc
    18541 agtaatatga tgttagcgaa aattggcgct cgtcgatgga tcgctgggat catggtggtc
    18601 tggggaattg cttccacctg cacaatgttt gcaacaagcc ccgaaacact ctatctactg
    18661 cgtatgctgg ttggtattgc tgaggcgggt tttttacctg gtattttggt ttatctgacc
    18721 tggtggttcc cggcatatca tcgggccaga gccaatgcct tatttatgat agcgatgcca
    18781 gtcactatga tgctgggatt gatcctttca ggctacattc tggccttgga tggactgtgg
    18841 aatctaaaag gctggcaatg gctctttttg ctggaaggtc tgccatcggt cattcttggt
    18901 gtcgtcacct ggttttatct caacgacaca ccagaccagg cgacctggct tgatgaaaac
    18961 gaaaaacagg ctttaaaaac gatgattctc cgtgaacaag agagtgcatt tgcccatgca
    19021 tctgttccac gctcaacctt acgtgaagtc ctgaccccag ccatcatgct atatacactt
    19081 gcttattttt gcccgactaa tactctgagt gcgattaata tctggacgcc gcaaatccta
    19141 cagagtttca atactggcag tagcaacatc atgattggtt tgctggcggc catcccccaa
    19201 ttctgcacaa tttttggcat gatctggtgg agtcgtcgtt ccgaccgtct taaagaacgc
    19261 aaaaagcata cgattcttcc ctacctgttt gccgctgccg gatggttact cgcttctgcg
    19321 acagatcaca gcctcattca actgagcagc atcattatgg cctcaatggg atcatttacc
    19381 gcgatgacta tcttttggac aacaccggat caagttataa gcttgcaatc gcgtgccgtt
    19441 gcgatggctg tcatcaatgc tgtcggcaat gtaggttcag cagtcagtcc attacttatc
    19501 ggtattttga aagatgcaac cggcagtttt agctcaggtt tgtggtttgt agcaggattg
    19561 ctggtactgg gcgcgttagt catgacccga atccctatgg ggagtcggga acactctctt
    19621 tcatctgaac ttatcgcacc aaaaccgcaa taaggagtca tgatgtccag tgaagctatc
    19681 tttgaacatg gttatgaaaa tattgatttg gtccgggaat atgatgcccg ctacgctggt
    19741 gaagatgtgc attatgaaac atttgcccgg ttagcgaatt tctttggacg cgatatgcgc
    19801 cctcactggc acgatagata ttttcaactc cattatctgg tcactggacg cattacttta
    19861 caactggatg agcacttcta ttctgtgcgc gcaccgcttt ttgtactaac accaccatcg
    19921 gtgccacata ctttctttac tgatcctgat agcgatgggc atgttctgac cgtacgtcag
    19981 gagttaattt ggcccctggt agaaaagctc tggcctggta gtggagatgc cgcaatgcta
    20041 caaggtatct gtctgtcact ggagggaatg gaacatacac tggcggcatt aaatcattat
    20101 tggccgataa tatccctgga gtttcaacaa aataagcaag gacgggaaat tctattgcaa
    20161 tcactggcgc aaagtatttt tacaatccta ctgcgtgaag caccaccaaa tgatatttca
    20221 acatgcagcg tgcgcggcga gatgtgttta ttccagaagt ttaatcggct gattgacgaa
    20281 aattatcgcc aacatttaat ggtccctgaa tatgcaacaa tgctcggtat gagtgaatca
    20341 cggcttaccg agctgtgccg acgttttgcc aatcaatcgc cgaagagatt gattttcgaa
    20401 cgagtgagcc gggaagccag acggcttctg ctctatagcg agcaaagcat caaccagata
    20461 gcccttgatc ttggctacaa agatcccgcc tatttcgccc gttttttcaa ccgaatgatg
    20521 ggttgctcac caacacaatt ccgtggtcgc tgaccattta tcaacctgag agactcgaca
    20581 tgaaaatatc tttacgctca ctatttgtgg cgtgcctgat tgcgccagtg gcattcacct
    20641 acgcctccga accgcaataa atgaaaattg tactacttgg aacgaaaggt ggcccttctt
    20701 tattaaaagc tgaccgttta ccgatggcaa cggcacttat tattggcaat aaaacctggt
    20761 taattgatgc tggttacggg gccagcttac aactggtaaa aaataatatt ccactgcgcc
    20821 agattgataa tattttactc acccatttac acagtgatca tattctggac tatccatcag
    20881 tcttaatgaa tgcatgggct agcggtctgc aggacaaaac tattcatgta ttcggtccgc
    20941 caggcacccg agccatgacc gctgccagtt ggcaggtctt cgatcgggac atcacactgc
    21001 gtatggaaga agaaggaaaa cccgatcctc gcaaactcgt tacagctcaa gatgttggtg
    21061 aaggcgtagt ttataaagat gataccgtga ccgtgactgc attaaaggtc cctcattctc
    21121 ctttccctga cggtgaggct tttgcttatc gcttcgacac acaaggcaag cgaattgtct
    21181 tctcaggaga tacctcgtac tttcccccac tggcaacttt ctcagaaggc gctgatatac
    21241 ttgtgcatga ggccgtacat gagccctccg tcgctaagct ggcagacagt attggcaatg
    21301 gtaaaacact ggcaaaagcc attgcttctc accatacgac tattgaggat gtaggcaaaa
    21361 ttgccaatga agcaaaagtg aaaaaactca tccttagcca cctggtacca atcaccgttc
    21421 cggatgaaac atggaagcaa gaagcacaga aaactattca ggtcctatcg tcgttggtca
    21481 tgaaaatatg acgattacca ttccattcgc cccataaaaa attccgtccc cgttagccct
    21541 tgttagcggg gaatattatg atttgcctta ctgtaagttg atcacgatcg cagtttacta
    21601 tttaactcca ccttgctgtc gaaaagtacc aacgttcgcc tgaaacgtct cttcccgaca
    21661 accgcgttca acgcttcaat taaataacaa aaacaacaca caaaattaac aatcgtattc
    21721 cgattaatac tgtagaggtc gacatgaaac cagaagattt ccgcgccagt acccaacgtc
    21781 ctttcaccgg ggaagagtat ctgaaaagcc tgcaggatgg tcgcgagatc tatatctatg
    21841 gcgagcgagt gaaagacgtc accactcatc cggcatttcg taatgcggca gcgtctgttg
    21901 cccaactgta cgacgcgctg cacaaaccgg agatgcagga ctctctgtgt tggaacaccg
    21961 acaccggcag cggcggctat acccataaat tcttccgcgt ggcgaaaagt gccgacgacc
    22021 tgcgccagca acgcgacgcc atcgctgaat ggtcacgcct gagctatggc tggatgggcc
    22081 gtaccccaga ctacaaagcc gctttcggtt gcgcactggg cgcgaatccg ggcttttacg
    22141 gtcagttcga gcagaacgcc cgtaactggt atacccgtat tcaggaaact ggcctctact
    22201 ttaaccacgc gattgttaac ccaccgatcg tcatttgccg accgataaag tgaaagacgt
    22261 ttacatcaag ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt
    22321 tgccaccaac tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat
    22381 gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtgaa
    22441 attaatctcc cgcgcctctt atgagatggt cgcgggtgct accggctcgc catacgacta
    22501 cccgctctcc agccgcttcg atgagaacga tgcgattctg gtgatggata acgtactgat
    22561 cccatgggaa aacgtgctga tctaccgcga ttttgatcgc tgccgtcgct ggacgatgga
    22621 aggcggtttc gcccgtatgt atccgctgca agcctgtgtg cgcctggcag tgaaactcga
    22681 cttcattacg gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg
    22741 tgtgcaggcc gatctcggtg aagtggtggc gtggcgcaac accttctggg cattgagtga
    22801 ctcgatgtgt tcagaagcga cgccgtgggt caacggggct tatttaccgg atcatgccgc
    22861 actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattataga
    22921 acgcaacgtt accagtggcc tgatctacct cccttccagt gcccgtgacc tgaacaatcc
    22981 gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg aacggtatgg atcacgtcca
    23041 gcgcatcaag atcctcaaac tgatgtggga cgccattggc agcgaatttg gtggtcgtca
    23101 cgagctgtat gaaatcaact actccggtag ccaggatgag attcgcctgc aatgtctgcg
    23161 ccaggcacaa agctccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc
    23221 ggaatacgac cagaacggct ggactgtgcc gcacctgcac aacaacgacg atatcaacat
    23281 gctggataag ctgctgaaat aacgcagcag gaggttaaga tgcaattaga tgaacaacgc
    23341 ctgcgctttc atgacgcgat ggccagcctg tcggcagcgg taaatattat caccaccgag
    23401 ggcgacgccg gacaatgcgg gattacggca acggctgtct gctcggtcac ggatacacca
    23461 ccgtcgctga tggtgtgcat taacgccaac agtgcgatga acccggtttt tcagggcaac
    23521 ggcaagttgt gcgtcaacgt cctcaaccat gagcaggaac tgatggcgcg ccacttcgcg
    23581 ggcatgacag gcatggcgat ggaagagcgt tttagcctct catgctggca aaaaggtccg
    23641 ctggcgcagc cggtgctaaa aggttcgctg gccagtcttg aaggagagat ccgcgatgtg
    23701 caggcgattg gcacacatct ggtgtatctg gtggagatta aaaacatcat cctcagtgca
    23761 gaaggtcacg gacttattta ctttaaacgc cgtttccatc cggtgatgct ggaaatggaa
    23821 gctgcgattt aagctccagc cccttcacgc attcgtcgaa ggggcatttt tattgtgatc
    23881 tcaatcacat atctacccga tgaataattc ttgcccaacc gctcaccgcc attcctgacc
    23941 gcttacatcc ctaaaataac cactcagtta tttaccttac tttacgcgca cgtatctctg
    24001 gcaacatcac tgcaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac
    24061 ggcttcggcc tatgattaac aaattactct atggattttg gtttgcagga aggcagcaag
    24121 tgagtgaatc cccgggagct tacaaaagta agtgtcagag gtgaacgaac gcagctgccg
    24181 cacctgtaag ccaaaagacg acgagtaaaa tgccaggttt tactatggat acgaaaaaac
    24241 tattcaagca cataccctgg gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag
    24301 ttgcattacg tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc tctgtgtcgg
    24361 tgtatctggt ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg atgaaactcg
    24421 accccacgcg cgcgacgcct gcggttatta acaacgacgg tctgaactac gttccgacca
    24481 accgttacgt gttgtttggt caccacttcg ccgctatcgc cggtgctggt ccgctggtgg
    24541 gaccggttct cgccgcgcag atgggctacc tgcctggcac gctgtggctg ctggcggggg
    24601 tagtgctggc cggtgcggta caggacttta tggtgctgtt tatctcctct cgccgtaacg
    24661 gcgcatctct cggtgagatg atcaaagaag agatggggcc agtaccgggg actatcgcgc
    24721 tgtttggctg tttcttaatc atgatcatca tcctcgccgt tctggcgctg attgtggtta
    24781 aagccctggc cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc
    24841 tgtttatggg tatctacatg cgctttatcc gtccggggcg tgtgggtgaa gtctctgtta
    24901 ttggtatcgt gctgctggtt gcctctatct acttcggtgg cgtgattgct cacgatccgt
    24961 actggggtcc ggcactgacc tttaaagaca ccaccattac cttcgcgctg attggctatg
    25021 cgtttgtttc cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa
    25081 ccttcctgaa aatcggcgtt atcgtcggcc tggcgctggg tatcgtggtg ctgaacccgg
    25141 aactgaaaat gcctgcgatg acccagtaca ttgacggtac tggcccgctg tggaaaggcg
    25201 ctctgttccc gttcctgttc atcaccatcg cctgtggtgc ggtatctggc ttccacgcgc
    25261 tgatctcttc cggtacgacg ccgaaactgc tggctaacga aaccgacgcg cgtttcattg
    25321 gctacggcgc aatgctgatg gagtccttcg tggcgattat ggcgctggtt gcagcgtcca
    25381 tcatcgaacc gggtctttac ttcgcgatga acaccccgcc tgctggcctt ggcatcacca
    25441 tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat catcatggcg cagctgaaag
    25501 acgttaccgc acacgcggca gcgaccgtca gctcctgggg cttcgtgatt tcgccagagc
    25561 agatcctgca aaccgcgaaa gacattggtg agccttctgt cctgaaccgt gcaggtggtg
    25621 cgccgacgct ggcggtaggt atcgctcacg tgttccacaa agtgctgccg atggctgaca
    25681 tgggcttctg gtatcacttc ggtattctgt tcgaagccct gttcatcctg accgcgctgg
    25741 atgcgggtac ccgttctggc cgctttatgc tgcaagacct gctgggtaac ttcatcccgt
    25801 tcctgaaaaa aaccgactct ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc
    25861 tgtggggcta cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt
    25921 ggccgctgtt cggtatctcc aaccagatgc tggcagccgt agcgctggta ctgggcaccg
    25981 ttgtgctgat taagatgaag cgcacccaat acatctgggt aactgttgtt ccggctgtat
    26041 ggctgcttat ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc aacccgcaga
    26101 tggaaggctt cttctacatg gcaagccagt acaaagagaa gattgctaac ggtactgacc
    26161 tgacggcgca gcagattgcc aacatgaacc acatcgttgt gaacaactac accaacgcag
    26221 gcctgagtat tctgttcctg attgtggtgt acagcatcat cttctacggt ttcaaaacct
    26281 ggcttgcggt gcgtaacagc gacaaacgta ctgacaaaga aacaccgtac gttccaatcc
    26341 cggaaggcgg cgtgaagatc tcttcgcacc actaaccgtg tttagccccg cttcggcggg
    26401 gctttgttct tatcagagtg aactatgttt ggtaacttag gacaggcaaa aaaatatctc
    26461 ggccaggcgg cgaagatgtt gattggtatt ccagactacg acaactacgt cgagcatatg
    26521 aagaccaacc atcccgacaa gccgtacatg agctatgaag aattcttccg cgaacgccag
    26581 aacgcgcgct acggcggcga tggtaaaggc ggtatgcgct gttgttaatg gagagagcat
    26641 gaacccgatt gcagttaccc tactcaccgg ttttttaggc gcgggcaaaa ctaccctgct
    26701 gcgccatatt cttaacgaac aacacggcta caagattgcc gtgattgaaa acgaattcgg
    26761 cgaagtctcc gttgatgacc aactgattgg cgaccgcgcc acgcagatca aaacgctgac
    26821 caacggctgc atctgctgtt cgcgctccaa cgagctggag gacgcactac tcgacctgct
    26881 ggataatctc gacaagggca atattcagtt cgatcgtctg gtgattgaat gcaccggcat
    26941 ggccgatccc ggcccgatta ttcagacctt tttctcccat gagattttat gccagctcta
    27001 cctgctggac ggcgtgattg cgctggtgga tgcggtacat gccgatgagc agatgaacca
    27061 gttcaccatc gcccagtccc aggtgggcta cgccgaccgt attctgctga ctaaaaccga
    27121 cgtcgcaggc gaagcagaga aactgcgtga acgcctggcg cgcatcaacg cccgcgcacc
    27181 ggtctacacc gtcacccacg gcgacatcga cctgggcctg ctgttcaaca ccaacggttt
    27241 tatgctggaa gagaacgtcg tcagcaccaa accgcgtttc cactttatcg cggataaaca
    27301 aaacgatatt tcgtcaattg tggtggaact ggattacccg gtagatatca gcgaagtttc
    27361 ccgcgtgatg gaaaacctgc tgctggaatc ggcggataaa ctgctgcgtt acaaagggat
    27421 gctgtggatt gacggcgaac ctaaccgcct gctgttccag ggcgtccagc gcctctacag
    27481 cgccgactgg gacaggccgt ggggcgatga aaaaccgcat agcacaatgg tgtttatcgg
    27541 gattcagttg ccggaggaag agattcgggc ggcgtttgcg ggattgagga agtaagttcg
    27601 ggaaagatca actttttgag agttccaccg caacattatt acgagtggaa ctctttagat
    27661 atgtcagacc aaccaaagct tcttcaattg cgttttcatt ttttcatgtc tcattttcag
    27721 ttcagccaat ccccattctg aagaattaca aacatctttt gttaaataaa aagagctttt
    27781 actattaagt ttctcataaa tagatttctt tttgataaaa tcatagtgtt gtgtttcaga
    27841 atttttggaa ccacttatga gtgttaaatt acctatttta tgcagccagt agacatgctc
    27901 ttgaggttgg aatctattaa tccagtattc attaactaat gcttgaggaa gaatatgctc
    27961 aattgtgatt ctccccgtat aggttttaat cacgctctca tcttgttgct ccatatctaa
    28021 gcgaagcaaa attgctttta ttaagttcac ttgattcgga cgaggctcat ataaatcttc
    28081 atccagagca gctataaaac ctgaattatc tgcatgttgg tttatctgat ttatcacaga
    28141 atcaaaaggc atatcattat ttattgctac cagagcagaa taacaaacca tttctctttg
    28201 gcttttaatt tgttttttta accacccatg catatatacc ttctcaaagg cggtaataaa
    28261 ttgagaaaaa tcatctaaat taaaatcttc tgtacgagcc attcgattca tgaatgccat
    28321 aaccggagga atccactcat ctacccctaa attacttagc gatgctattt ttcgtcgtat
    28381 tgaagggtgt tcaaaatcat tctctaaaat cttagtata
//
LOCUS       DAJTSF010000049        28404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000049 DAJTSF010000000
VERSION     DAJTSF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.049"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OO335_002219"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OO335_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR6002590.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /gene="dcuS"
                     /locus_tag="OO335_002220"
     CDS             321..1952
                     /gene="dcuS"
                     /locus_tag="OO335_002220"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6002591.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="OO335_002221"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="OO335_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6002592.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="OO335_002222"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="OO335_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6002593.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="OO335_002223"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="OO335_002223"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6002594.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="OO335_002224"
     CDS             6426..7055
                     /locus_tag="OO335_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6002595.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OO335_002225"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OO335_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR6002596.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8718..10073)
                     /gene="melA"
                     /locus_tag="OO335_002226"
     CDS             complement(8718..10073)
                     /gene="melA"
                     /locus_tag="OO335_002226"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR6002597.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="OO335_002227"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="OO335_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR6002598.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11463..13730
                     /gene="adiA"
                     /locus_tag="OO335_002228"
     CDS             11463..13730
                     /gene="adiA"
                     /locus_tag="OO335_002228"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6002599.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14055..14816
                     /gene="adiY"
                     /locus_tag="OO335_002229"
     CDS             14055..14816
                     /gene="adiY"
                     /locus_tag="OO335_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6002600.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14953..16290
                     /gene="adiC"
                     /locus_tag="OO335_002230"
     CDS             14953..16290
                     /gene="adiC"
                     /locus_tag="OO335_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6002601.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16394..18037
                     /gene="eptA"
                     /locus_tag="OO335_002231"
     CDS             16394..18037
                     /gene="eptA"
                     /locus_tag="OO335_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6002602.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18034..18702
                     /gene="basR"
                     /locus_tag="OO335_002232"
     CDS             18034..18702
                     /gene="basR"
                     /locus_tag="OO335_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6002603.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18703..19803
                     /gene="pmrB"
                     /locus_tag="OO335_002233"
     CDS             18703..19803
                     /gene="pmrB"
                     /locus_tag="OO335_002233"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6002604.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="OO335_002234"
     CDS             complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="OO335_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6002605.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19980..21482)
                     /gene="proP"
                     /locus_tag="OO335_002235"
     CDS             complement(19980..21482)
                     /gene="proP"
                     /locus_tag="OO335_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6002606.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21746..22624)
                     /locus_tag="OO335_002236"
     CDS             complement(21746..22624)
                     /locus_tag="OO335_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR6002607.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="OO335_002237"
     CDS             complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="OO335_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6002608.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25252..25587
                     /locus_tag="OO335_002238"
     CDS             25252..25587
                     /locus_tag="OO335_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6002609.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25745..26188
                     /gene="yjdN"
                     /locus_tag="OO335_002239"
     CDS             25745..26188
                     /gene="yjdN"
                     /locus_tag="OO335_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR6002610.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26321..27109
                     /gene="phnC"
                     /locus_tag="OO335_002240"
     CDS             26321..27109
                     /gene="phnC"
                     /locus_tag="OO335_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6002611.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27134..28150
                     /gene="phnD"
                     /locus_tag="OO335_002241"
     CDS             27134..28150
                     /gene="phnD"
                     /locus_tag="OO335_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6002612.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28256..>28404
                     /locus_tag="OO335_002242"
     CDS             28256..>28404
                     /locus_tag="OO335_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR6002613.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7297 a         7417 c         6935 g         6755 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg agttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcca ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tacgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaacaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aatcgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcgc ctggcagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac tggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataactg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac cagcttggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgatg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctagct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggtcat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaagattc cttcataacc gggtaagcaa
    11461 tgatgaaagt attaattgtt gaaagcgagt ttctccatca agacacctgg gtcggtaacg
    11521 ccgttgagcg tctggcagat gctttaagcc agcaaaatgt taccgtgatt aaatccacct
    11581 cctttgatga tggttttgcc attctctctt caaacgaagc cattgactgc ctgatgttca
    11641 gctatcaaat ggagcatccg gacgaacatc aaaacgtcag acaattgatc ggtaagcttc
    11701 atgagcgcca acaaaacgtg ccggtcttcc tgttgggcga tcgggaaaaa gccctcgccg
    11761 caatggaccg ggatctgctg gagcttgtcg atgaattcgc ctggattctg gaagataccg
    11821 ccgactttat cgccggacgc gccgttgccg cgatgacccg ctaccgccag cagctgttgc
    11881 cgccactgtt cagcgcgctg atgaaatata gtgacatcca tgaatattcc tgggcagcgc
    11941 caggccacca gggcggcgtt ggttttacca aaacacccgc cggacgtttc taccatgact
    12001 actatggtga aaatctgttc cgtaccgaca tgggcatcga acgaacttcc ctcggttctt
    12061 tgcttgacca tactggcgca tttggcgaaa gcgaaaaata tgccgcacgc gtatttggtg
    12121 ccgatcgctc ctggtcggta gtcgtcggta cttccggctc taaccgcacc atcatgcagg
    12181 cttgcatgac cgataacgat gtcgtggtcg ttgaccgtaa ctgccataaa tccatcgaac
    12241 aaggtttgat gctgacaggc gcgaaaccgg tctatatggt gccaagccgc aaccgctacg
    12301 gcattatcgg gccaatctat ccgcaggaaa tgcaacctga aaccttgcaa aagaaaatca
    12361 gtgaaagccc gctgaccaaa gacaaagccg ggcaaaaacc gtcttactgc gtggtaacca
    12421 actgcaccta tgacggcgtg tgttataacg ctaaagaagc gcaggatctg ctggaaaaaa
    12481 cctccgatcg tctgcacttt gacgaagcct ggtacggcta tgcacgtttc aacccgatct
    12541 atgccgatca ctatgccatg cgcggcgaac ctggcgatca caacggtcct accgttttcg
    12601 ccacccactc catccacaaa ctgctgaatg cgctgtcaca ggcttcttat attcatgtac
    12661 gtgaaggtcg tggggcgatt aacttctccc gcttcaacca ggcctacatg atgcatgcca
    12721 ccacctcccc gctgtatgcc atctgcgcat ccaacgacgt ggcggtgtcg atgatggacg
    12781 gcaacagcgg cctgtcactg acacaggaag tgattgacga agcggttgat ttccgtcagg
    12841 cgatggcgcg gctatataaa gagttcaccg ctgacggtag ctggttcttc aaaccgtgga
    12901 acaaagaagt cgtcaccgac ccacaaaccg gcaaaaccta tgactttgct gacgcaccaa
    12961 ccaaactgct gaccaccgtt caggactgct gggtaatgca tccgggcgaa agctggcacg
    13021 gcttcaaaga tattccggat aactggagta tgctcgaccc aattaaagtc agcatccttg
    13081 ctccgggaat gggtgaagat ggtgaactgg aagaaaccgg tgttccggcg gcgctggtca
    13141 ctgcctggct tggtcgccac ggcattgtgc ctacccgcac cactgacttc caaattatgt
    13201 tcctgttctc tatgggcgta acccgtggga aatgggggac tctggttaac accctttgct
    13261 ccttcaaaca ccactatgac gccaacacac cgctggcgca ggtgatgccg gaacttgttg
    13321 aacaatatcc tgacacttac gcgaacatgg ggattcacga tctgggtgac accatgtttg
    13381 cctggctgaa agaaaacaac cctggcgcac ggttgaacga agcctattcc ggcctgccgg
    13441 tggcggaaat aaccccgcgt gaagcgtaca acgcgattgt cgacaacaat gtcgaactgg
    13501 tatccattga aaatctgcca ggacgcatcg cggcaaactc agttatcccg tatccgccag
    13561 gaatcccgat gctgctgtct ggtgaaaact tcggcgataa aaacagtccg caagtaagtt
    13621 atttacgctc gctgcaatcc tgggaccacc atttccctag atttgaacac gaaactgaag
    13681 ggactgaaat tattgacggt atttaccacg ttatgtgcgt gaaagcgtaa ccactattcc
    13741 gctgaaggcg taattgttta aataacatta cgccgcctgg ccttaggcct tttgagtatg
    13801 gcaacgtttt cataaaaatt gctgcaaaca aaaatgtcat actttttgcg cggccccacc
    13861 ccacgctttt ttttgcctgt tatttatcct gtaaaaaaat atgtacatga gaaattacta
    13921 taaaaatttg taatattagt aaaactcgtt atttttatgc atgtttatat tcatcatgca
    13981 attatataac catttcccgg tatcgctttg ctttagcgag aactggtctt ttattcgcac
    14041 tcaggagtac atgtatgagg atttgcagcg accaaccttg tattgtttta ttgactgaaa
    14101 aagatgtctg gataagggtg aatgggaaag aacctattag ccttaaagct aaccatatgg
    14161 cgttattaaa ttgtgaaaat aatattatcg acgtctcctc tcttaacaac actttggttg
    14221 ctcatattag tcacgacatc atcaaagatt acctccggtt tctgaataaa gatctctcgc
    14281 aaataccagt atggcaacgt agcgctacgc ccatactcac cctgccatgc ctgacgccag
    14341 acgtctttcg cgttgccgcg caacacagca tgatgcccgc agaaactgag tcagaaaagg
    14401 aacgaacacg cgcattatta ttcacggtgc tatcccgttt tctcgacagt aaaaaattcc
    14461 tttcattaat gatgtatatg ttacgtaatt gtgtaagtga cagcgtttat caaattattg
    14521 aaagcgatat tcacaaagac tggaatctta gtatggtagc cagttgttta tgtcttagcc
    14581 caagtctgtt aaagaaaaag ttgaaaagcg aaaacaccag ttatagccaa ataatcacca
    14641 cctgccgcat gcgttatgcc gtaaatgaat taatgatgga cggtaaaaat atctctcagg
    14701 tatcacagtc ctgcggctac aacagtacgt cgtactttat ttctgtcttt aaagacttct
    14761 acggtatgac gccgctgcat tatgttagtc agcacagaga acgcactgtc gcctgatttt
    14821 taaccttaac gaagagctat attaataacg gcatcagcga taacccggtc gataataatt
    14881 caactatcga atgcaggcgt atgatatgac gtaatttatt gtcacgaagc tcgccttcgc
    14941 aggagtttaa ttatgtcttc ggatgctgat gctcacaaag tgggcttaat ccccgtcacc
    15001 ctgatggtgt cggggaatat tatggggtca ggtgtttttc tgttacctgc aaacctggcc
    15061 tccactggcg ggattgccat ttatggatgg ttggtgacga ttatcggtgc gctggggctc
    15121 tcgatggtat acgccaaaat gtcgttcctc gacccaagtc ctggtggttc ttacgcttac
    15181 gcccgccgct gctttggccc gtttctcggt tatcaaacca acgtcctcta ctggctggcc
    15241 tgctggatcg gcaatatcgc catggtggtc attggcgtag gatatttaag ttacttcttc
    15301 ccgattctga aagatccatt ggtattaacc atcacctgcg tcgtggtgct gtggatcttc
    15361 gtcctgctga acattgtcgg tccgaaaatg atcacccgcg tgcaggccgt tgccactgtg
    15421 ctggcgctga tccccatcgt cgggattgcc gtatttggct ggttctggtt ccgtggtgaa
    15481 acctatatgg cggcatggaa cgtcagcggc ctgggcacct tcggtgcgat tcaaagtacc
    15541 cttaacgtta cgctgtggtc gttcatcggt gtggaaagtg cctccgttgc cgcaggtgtg
    15601 gtgaaaaacc cgaaacgcaa tgtccctatc gccaccattg gtggggtatt gattgccgcc
    15661 gtttgctatg tgctctccac caccgcgatt atggggatga tccctaatgc cgcactgcgc
    15721 gtttctgcct cgccattcgg tgatgccgca cggatggcgt tgggtgacac cgctggggcc
    15781 attgtttcct tctgcgcagc tgcgggttgc ttaggttcac tgggcggctg gacgttgctg
    15841 gcgggtcaaa cggcgaaagc cgctgccgat gacggactgt tcccaccgat ttttgcccgt
    15901 gtaaataaag cgggtacgcc agtggcgggg ctgattatcg tcggtatttt gatgaccatc
    15961 ttccagctca gcagcatttc accaaacgcg accaaagagt tcggtctggt ttcttccgtc
    16021 tcggtcatct ttacactggt gccatatctt tacacctgtg cggcgttact gctgctcggc
    16081 cacggtcact ttggtaaagc acgcccggca tatctggctg ttactaccat tgccttcctc
    16141 tactgcattt gggccgtggt ggggtccggg gcgaaagagg ttatgtggtc atttgtcacc
    16201 ctgatggtca tcaccgccat gtatgcgctg aattacaacc ggctacataa aaacccgtat
    16261 cccttagatg caccaataag caaagattaa ttccccttaa tccagcaaac ataaaagcca
    16321 accttaagaa cttaaggttg gcttaatttt gctttgcgag catatgcgca ctttgttcga
    16381 tggaaacacc gtgatgttga agcgcctact aaaaagaccc tctttgaatt tactcgcctg
    16441 gctattgttg gccgcttttt atatctctat ctgcctgaat attgcctttt ttaaacaggt
    16501 gttgcaggcg ctgccgctgg attcgctgca taacgtactg gttttcttgt cgatgccggt
    16561 tgtcgctttc agcgtgatta atattgtcct gacactaagc tctttcttat ggcttaatcg
    16621 accactggcc tgcctgttta ttctgattgg cgcggctgca caatatttca taatgactta
    16681 cggcatcgtc atcgaccgct cgatgattgc caatattatt gataccactc cagcagaaag
    16741 ttatgcgctg atgacaccgc aaatgttatt aacgctggga ttcagcggcg tgcttgctga
    16801 gctgattgcc tgctggctaa aaatcaaacc tgccacctcg cgtctgcgca gtgttctttt
    16861 ccgtggagcc aatattctgg tttctgtact gctgattttg ctggtcgccg cactgtttta
    16921 taaagactac gcctcgttgt tccgcaataa caaagagctg gtgaaatcct taagcccctc
    16981 taacagcatt gttgccagct ggtcatggta ctcccatcag cgactggcaa atctgccgct
    17041 ggtgcgaatt ggtgaagacg cgcaccgcaa cccgttaatg cagaacgaaa aacgtaaaaa
    17101 tttgaccatc ctgattgtcg gcgaaacctc gcgggcggag aacttctccc tcaacggcta
    17161 cccgcgtgaa actaacccgc ggctggcgaa agataacgtg gtctatttcc ctaataccgc
    17221 atcttgcggc acggcaacgg cagtttcagt accgtgcatg ttctcggata tgccgcgtga
    17281 gcactacaaa gaagagctgg cacagcacca ggaaggcgtg ctggatatca ttcagcgagc
    17341 gggcatcaac gtgctgtgga atgacaacga tggcggctgt aaaggtgcct gcgaccgcgt
    17401 gcctcaccag aacgtcaccg cgctgaatct gcctgatcag tgcatcaacg gcgaatgcta
    17461 tgacgaagtg ctgttccacg ggctggaaga gtacatcaat aacctgcagg gtgatggcgt
    17521 gattgtctta cacaccatcg gcagccacgg tccgacctat tacaaccgct atccgccgca
    17581 gttcaggaaa tttaccccaa cctgcgacac caatgagatc cagacctgta ccaaagagca
    17641 actggtgaac acttacgaca acacgctggt ttacgtcgac tatattgttg ataaagcgat
    17701 taatctgctt aaagaacatc aggataaatt taccaccagc ctggtttatc tttctgacca
    17761 cggtgaatcg ttaggtgaaa atggcatcta tctgcacggt ctgccttatg ccatcgcccc
    17821 ggatagccaa aaacaggtgc caatgctgct gtggctgtcg gaggattatc aaaagcggta
    17881 tcaggttgac cagaactgcc tgcaaaaaca ggcgcaaacg caacactatt cacaagacaa
    17941 tttattctcc acgctattgg gattaactgg cgttgagacg aagtattacc aggccgcgga
    18001 tgatattctg caaacttgca ggagagtgag taaatgaaaa ttctgattgt tgaagacgat
    18061 acgctgttat tgcagggact gattctggcg gcgcaaaccg aaggctacgc gtgcgatggc
    18121 gtgacaaccg cgcggatggc ggaacaaagc cttgaggacg gtcattacag cctggtggta
    18181 ctggatttag ggttacccga cgaagatgga ctgcattttc tcgcccgtat ccggcagaaa
    18241 aaatacaccc tgccggtact gatcctcacc gctcgcgata cgctgaccga caaaatcgcc
    18301 gggctggatg tcggtgccga tgactatctg gtgaagcctt ttgcgctgga agagttacac
    18361 gcccgtatcc gcgccctgct acgacgccat aataatcagg gcgaaagtga gctgattgtt
    18421 ggcaatctga cgctgaatat gggtcgccgt caggtatgga tgggcggtga agagttgatt
    18481 ctgacgccca aagaatatgc tctgctgtca cggttaatgc tcaaagcagg cagtccggta
    18541 catcgggaaa ttctctacaa cgacatctat aactgggaca atgaaccctc gaccaacacc
    18601 ctggaagtgc atatccacaa tctgcgcgac aaagtgggca aagcccgtat ccgcaccgtg
    18661 cgcggctttg gctatatgct ggtcgcgaat gaggaaaact aattgaatct gatgcgtttt
    18721 ctgcgccgac ctatatcgct gcgccaacgg ctgatattga ccatcggggc cattttgttg
    18781 gtgtttgagc tgatcagcgt cttctggcta tggcatgaaa gtaccgagca gattcagctg
    18841 tttgagcagg cgctacgcga caatcgcaac aacgatcgcc acatcatgcg tgagatccgc
    18901 gaggcggtcg ccagcctgat tgtccccggc gtctttatgg tcagcctgac gctatttatc
    18961 tgctatcagg cggtacgccg catcacccgc ccgctggcgg agctgcaaaa agagctggaa
    19021 gcgcgcaccg ccgacaactt aacgcccatt gccattcaca gcgccaccct cgaaatcgaa
    19081 gcggtggttt cggcgttgaa cgatctggtc agtcgcctga ccagcacgct ggataacgaa
    19141 aggttgttta ccgctgacgt cgcgcacgaa ctgcgaacgc cactggcggg ggtgcgtttg
    19201 catctggaac tgctggcgaa aacgcatcac attgatgtag caccgttagt ggcacggctt
    19261 gatcagatga tggagagcgt ctcccagctg ctgcaactgg cgcgtgccgg acagtcattt
    19321 tcttccggta attatcaaca tgtaaaactg ctggaagatg tcattctccc ctcgtatgac
    19381 gaactcagta ccatgctcga ccagcgacag caaaccctgc tattgccaga gagcgccgcc
    19441 gacatcaccg ttcagggtga tgcaaccctg ctgcggatgt tattgcgaaa cctggtagaa
    19501 aacgcccatc gttacagccc gcaaggcagc aacattatga ttaagctgca agaagatggc
    19561 ggagcggtca tggcagttga agatgaagga ccaggtattg atgagagtaa atgcggggag
    19621 ttgagtaaag cgtttgtacg tatggacagc cgttatggcg ggattggtct ggggttaagt
    19681 attgtcagcc gcattacaca gttgcatcac gggcagtttt tcctgcaaaa ccggcaagag
    19741 acttccggca cgcgggcctg ggtacggctg aagaaagatc agaacgtggc aaaccagata
    19801 taaagaaagc tgctgaccac cagcacgctg aacacggtag ttaaactttc ataaacacgg
    19861 tttttcattg cactctcctc cgttaacctg gaggagagta tgcgcgtcag agattaagtg
    19921 aaccttaaga gttcaattgg cctgataaga cagcgtcaca tcaggccatc cgtttcagct
    19981 tattcatcaa ttcgcggatg ttgctgcacc aggcgggtac gtttcgcctg caaatcggca
    20041 atctcgtggt caatattatc gattttctgc tcgatattat cgtaatgctc gacgagaatt
    20101 tccttcgctt cctgtatatc tgacgccgcc ggtgtcgcac ctttcaacgg acgatttgcc
    20161 gtctctttca tggttacgcc ggtgattaaa ccaatcaccg ccactaccat caggtaatag
    20221 gcaggcatca tcagattctg cgagctttcg accagccagg ccgccagtgt tggcgtcaga
    20281 ccggcaacca gcaccgaaat attaaatgcc gccgccagcg cgctgtaacg gatatgcgtc
    20341 gggaacatcg ctggcaaggt agaagccata acgcccataa agcagttaag gatcaccgcc
    20401 agcatcagta acccggcaaa aatcaggccg atgacgttac tgttaatcag aataaacgcc
    20461 gggatcgcca acacaaacag tgcaacacta ccaagtagca caaacggacg acggccaaaa
    20521 cggtcactca gcaagcccat caccggctgg acaaacagca taccgatcat aatggcgata
    20581 ataatcagca ccccgtggtc ttcggagtaa tgcaggttat gcgacaaata actcggcata
    20641 taggtcagca acatgtagta agtcacgttg gtggcaatta ccagaccaat acatgtcaac
    20701 aggctgcgcc agtatttagt ggcaatctct ttaaacgaga ctttcgggcc atcctgcaaa
    20761 ccttcgcggt cgccctgttc cagtttatca acatgctgct ggaacgccgg agtttcttcc
    20821 agcgcatggc gcaggtaaag cccgataatc cctaacggca gagcaataaa gaacggaata
    20881 cgccagcccc agtcgaggaa gttcgcttcg ccgacaatgg tcgaaattaa caccaccacg
    20941 cccgcaccca gcacaaaccc ggcaatagaa ccgaagtcca gccagctgcc cataaagcca
    21001 cgtttacggt caggggagta ttccgcaaca aatatcgacg ccccggtata ttcaccaccg
    21061 accgagaaac cttgtgccat cttacagatc aacagcagaa tcggtgccca aataccaatc
    21121 gtgtcgtagg acggtattaa gccaatacag aacgtactga tcgacataat cacaatagtg
    21181 atagcgagga tcttctggcg accatattta tcgcccaaca taccaaagaa gagtccgcca
    21241 agcggtcgaa tcagaaaggg aacggagaaa gtggcaagtg cagcaaccat ctgcacgctg
    21301 gggtcagccc ccgggaaaaa aactttacct aatgcgtaag caacaaaacc ataaacacca
    21361 aaatcaaacc attccattgc attacccagt gatgctgcgg taatggcttt acgcagttta
    21421 ccgtcatcaa taatggtgac atcacgaagg gtaatcggtt ttactttttt ccttttcagc
    21481 atagctttcc tcgcagagtt ggcccatttc cgcaaacgcc agaaaaatca gcgcgacggc
    21541 cgcaaaaggg gagcgggaat tgcaggatgc aatctctgta acaagcgtag cgggtttgct
    21601 tacaccctcc ggttaaggaa tgtacaatca aaccagttga cgcctgaatt gggcagttaa
    21661 tgaccttttt accctaccag tgtttaaatt tgtgatcaac ttcacacatg gatttcgttt
    21721 aggactcatt gatgtaactg attatttacg ccaaatttac agagaagatt tcctcgacca
    21781 ttgcccccac caaacgctgc gctgaacaga gtcgtggcat ccccagcgcg acagtttgcc
    21841 agcgttgggt cactgaccag caaacgggtt gtttatctgc cgtggtccac tttcccagcc
    21901 aacttaaaaa atcagcatga tccccgcccg tcggaacggt cgaagaggca aaccactgat
    21961 ggtaatagtg acgcgtagtc ggtgccgcat tgatactctc tgccagatgt tgtgtcgctt
    22021 tctcgcgcaa atcacccagc aacaacacca ccgcttgcgg gcttcccaga cggcaggcat
    22081 cgccaaatgc accccagcgc aactcctcca gaacgataaa acagcgccca ggcaacgacc
    22141 aggcgttata cgccccagca cgccagcggg taaaaatttg ctcgctctga tcacctgcct
    22201 gcaccagtag gcctcgttgc gttaacgcct tttccttcca gcgacaacgg gctgaaaacc
    22261 actcatacaa ggcggcaatt tgctggggga gctccgggaa agcagagtgc tggcggtgat
    22321 ccattttttg cagcagcgcc agatgctgcg cgatctgcgt cagcgccaga tgaccgggat
    22381 ccagcatcga cgccagtttt tccaccattg tcagtcgcag gctaagcggc gcatttaaca
    22441 tgccgccgtt cgtccatgtc tgaagctgca cacgcgaaag aagttcctgc atcagtcgtt
    22501 cacgaaactg ctgctgatgg gcttgaggat ggcgggcatc atcgccctgc gccagatcca
    22561 ctaaaaagcg cggatgaacc gactccagca cgcgaccggg gccgtccagt aacgtcttgg
    22621 tcaatatcgt tctgccgtga aaagtgtttg aatatcatcg cgtaacagct gagcgtcggt
    22681 gtaaatccag ctgtgagtca tcacagtctg ctgcaattgc tgctgcatca gcctgaccac
    22741 cgattcattt tgttgacgca gagccagact ttcgcgtaaa cgcgtctgta attctgtcaa
    22801 acatgaagcg aactcagcga aaaaagtatt catgcctgcc gtaacagatt catcgacctg
    22861 ctctgccaga actttagcca tttgttggca atagagatcg acttctgcgc ttaatgctcg
    22921 ttgcaacaca ctgtaatcaa ccgtttctgt cggggatttc tcatttcccc gtccccagtc
    22981 gggctgattc aaccagcgcg aaaaagtctc acgcacaacg cctaaacgcg tgctctgctc
    23041 gtccgttgct tcctggcgcg aaatgacttc actgaacagc tggcgagtgt tgaagtgggg
    23101 aactacgccg tgaaaaacag gaaaatgaaa cccaggacga aaccctgact cgctcaattc
    23161 cattttgact tgttgctcaa tggggcgaat aacatcggtt aacacccggc aaagggtgga
    23221 ttccatctcg gcaaaacgca gcgtaaagtc gcgactgatg gtgttctgcg ccgtctgtaa
    23281 cagtgtctca cagcgggtac gaatctcgct taacggctcc gaatcatcct gaaacaaggc
    23341 ggctaactgc gcattcaccg catcttgctg ttgacgcaga aagtggttgg cggaggtcag
    23401 ggccagctcg atttcatgtt taatctcgcc gctcacctgc gcctgattga gttgcaatag
    23461 ctgcaaactt tcttcgacct gatggatatt ttgccgcaat tgttcacaag cgacgtttaa
    23521 cccgtgcgca cgaaaatcca ggtattcccg cgcctgctgc gcgtaattca acagtttatg
    23581 cgcagcagat cgcagagcat acaatgaggc gttagcgtaa gcggcatgaa gcaacgcctg
    23641 aattggctgg gcgaacagcg aatcttccca caactgatcg gcagcatgac gaatatgttc
    23701 gaggtccgcc agatcggcat gacgccagcg cctgccgagc gcggcatggg caaaatcttc
    23761 cacccagcgt tgttgctctg gcgctggtaa cttaccgttg ttggctaact catagcgcgc
    23821 ccgattcgcc aggtagcccc acatcgacga caccgggaat atctgctgtg gcgtaataca
    23881 gcctttcatc agcgtcccgg aaatcagtgc ccgcacctgg tcggcgtcgt cactgttacg
    23941 atcctgttga tcgaacttat tgaccagcac atacagcggc accgattgcc ccaccgccaa
    24001 aatcgcctca cggacctctt catcggagat cgatttcagt tgcgtataat ccagcaccgc
    24061 cagtaccgcc gaggcgcgtg ccagctgctg gttaagcatt ttttgcagat gcggttgccc
    24121 ggcttcattt ggcccggggg tatccagtaa cgtcaactga ccgggataac tttccagccc
    24181 cgccagatgg acaaactcca cttcaatcac gggaatatgc tcaatggcgg cgtaagcaga
    24241 aaaaggaaaa tcgacgtcca gcgccttcgc cagtcgcact aaatcattca aacttttcag
    24301 acaatgaaaa ataggctggg cacccagata atatttttcg aaagcgacgc cattttcgat
    24361 ccgctgcata agcgcacgca tatctttatc tatttccagc acatcggtca gatgcttaat
    24421 atcgcaatca cgcaggcgct gttgtaattg ttgaattaaa caatcgattg gcgcgacatg
    24481 tgaaaaatgc agtaccggtt ccttttgccc gggcgtatgg cgaataagcg tcggcagcgc
    24541 agtcattggg cgattacgat taggcagaac ctccgtacca acaatggcat taatggtggt
    24601 tgatttccct gctttcatgg taccgacaat tgccagcacc atttccagtc gggaaatttt
    24661 acacaactca ttattcagca tcgcgtgacg ttcggcgata ttaggctgac tccagggtaa
    24721 agccagttgt ggcgcgtcgt ctccgggtac agagagaggc attttttcca gtaactgcaa
    24781 ctgttggcga gaaagctgta acaggcgttc agcctcctga cttaactcat acagggtctg
    24841 tgtgtacata gaaaattctt ccttaaagca aattttgtta cttttattta gccagattgt
    24901 ttttgagttc tgttttcggc ttttataatt actgcaagaa ataattttat atttagtgtg
    24961 ttgttttttt atcagaataa ataacgtctt ctgatgcgtt taaaacgtca gaaagataaa
    25021 aatatcatgt gaataaaaaa aagaacaagt agagcattaa cattatctta aataataaat
    25081 agaggcaaaa agattatttt ctttttgcgt ttcctttcaa atgaaaacga tcgtcgtcta
    25141 aaatcagcag tacccccgac aaactcaggg attttgtgta taattgcggc ctttttcggc
    25201 aatctgccgt tttttggcgc ttttgccctg ctgacttttg aggaaatcca catgtcatta
    25261 ccacactgcc caaaatgcaa ctccgaatac acttacgaag ataacggcat gtacatctgc
    25321 ccggaatgtg cctacgaatg gaacgacgca gaacctgcac aggaaagcga cgagctgatc
    25381 gttaaagatg ctaacggcaa tctgctggct gacggcgaca gcgttaccat cattaaagat
    25441 ctgaaggtga aaggtagctc ttcgatgctg aaaattggca ccaaagtgaa aaacatccgc
    25501 ctggttgaag gcgaccataa catcgattgc aaaatcgacg gttttggtcc gatgaaactg
    25561 aaatctgagt ttgtgaaaaa gaactgattg tattgtgatc ggtaagccgg ataaggcgct
    25621 cgcgccgcat ccggcaacga tgccgactgc ctgatgcgac gcttgcgcgt cttatcaggc
    25681 agcctctcat ctgtataaat ttcgaactac acttaactgg cttctcttaa ctgaggtcac
    25741 catcatgccg ttaagtccct acctctcttt tgccggtaac tgttccgacg cgattgccta
    25801 ttatcaacgt acgttgggcg cggaactgct ctataaaatc agcttcggcg aaatgccgaa
    25861 atcagcgcag gacagcgccg agaactgccc ttccggaatg caatttcccg ataccgccat
    25921 cgctcatgcc aacgtgcgca ttgccggaag cgacatcatg atgagcgatg ctataccgtc
    25981 aggaaaagcc agctactccg gctttacgct ggtgctcgat tcgcaacagg tcgaagaagg
    26041 aaaacgctgg tttgataacc ttgccgctaa cggaaaaatc gaaatggcct ggcaggaaac
    26101 tttctgggcg catggctttg gcaaagtcac cgataaattt ggcgtaccgt ggatgattaa
    26161 tgtcgtcaaa caacaaccaa cgcaataacc cgccgggagg cccgccctcc cgcactgtca
    26221 tcgaattccc gttaactctt catctgttag tcacttttaa ttaaccaaat cgtcacaata
    26281 atccgccacg atggagccac ttttttaggg aggctgcatc atgcaaacga ttatccgtgt
    26341 cgagaagctc gccaaaacct tcaatcagca tcaagcgctg catgcggttg atctgaacat
    26401 tcatcacagt gaaatggtgg ctctgcttgg gccgtcgggt tccggcaaat ccaccctttt
    26461 acgtcattta agcggtttga ttaccggcga taaatccgcc ggtagccata tcgagctact
    26521 gggccgcact gtccagcgcg aaggccgcct ggcccgcgat atccgcaaaa gccgcgccaa
    26581 caccggctac atctttcaac aattcaacct ggtgaaccgc ctgagcgtac tggagaacgt
    26641 gctgattggc gcgctcggca gcacgccgtt ctggcgcacc tgttttagct ggttcaccgg
    26701 cgagcagaaa cagcgcgcgt tacaggcgct gacccgcgtt ggcatggtgc attttgccca
    26761 tcagcgcgtt tccaccctct ccggcggcca gcagcaacgt gtggcgattg cccgtgcgct
    26821 gatgcagcag gcgaaagtga ttctggccga tgaacccatc gcctcgctgg acccggaatc
    26881 agcgcgcatc gtgatggaca ccctgcgcga catcaaccag aacgacggca tcaccgtggt
    26941 cgtcacgctg catcaggtgg attacgccct gcgctactgc gaacgcatcg tcgccctgcg
    27001 tcaggggcac gtcttctacg acggcagcag ccaactgttt gataacgaac gttttgacca
    27061 tctctaccgc agcattaacc gcatcgaaga gaacgcgaaa gctgcctgat atccccatca
    27121 ttgaggaaaa cgaatgaacg ctaagataat tgcctcgctg gccttcacca gcatgttcag
    27181 cctcagcacc ctgttaaccc cggcacacgc cgaagagcag gaaaaggcgc tgaatttcgg
    27241 cattatttca acggaatcac agcaaaacct gaaaccgcaa tggacgccgt ttttacagga
    27301 tatggagaag aaactgggcg tgaaggtcaa cgccttcttt gccccggact acgcgggcat
    27361 tatccagggg atgcgcttca ataaagtgga tattgcctgg tacggcaacc tgtcggcgat
    27421 ggaagcggtg gatcgcgcca atggccaggt cttcgcccag acggtcgcgg cggatggatc
    27481 gccgggttac tggagcgtgt tgatcgtcaa caaagacagc ccgatcaaca acctgaacga
    27541 tctgctggcg aagcggaaag atctcacctt cggcaatggc gatcctaact ccacctctgg
    27601 cttcctcgtc cccggttact acgtcttcgc taaaaacaat atctccgcca gcgacttcaa
    27661 gcgcaccgtc aacgccgggc atgaaaccaa cgcgctggcc gtcgccaaca agcaagtgga
    27721 tgttgccacc aacaacaccg aaaacctcga caagctgaaa acctccgcgc cggagaagct
    27781 taaagagctg aaggtgatct ggaaatcgcc gctgatccta ggcgatccga tcgtctggcg
    27841 caagaatctg tccgaaacta ccaaagacaa gatctacgac ttctttatga actacggaaa
    27901 aacgccggaa gagaaagcgg tgctggaacg cctgggctgg gcgccgttcc gcgcttccag
    27961 cgacctgcaa ctggtgccga ttcgccagct cgcactgttt aaagagatac aaagcgtgaa
    28021 aggcaataaa gggctgaatg agcaggacaa gctggcgaaa accaccgaga ttcaggcgca
    28081 gctggatgac ctggaccgcc tgaacaacgc gttaagcgcg atgagttcgg tgagtaaagc
    28141 ggtgcagtaa atcgtaggtc ggataagacg ccccggcgtc gcatccgaca atgtgtaggc
    28201 gttgatgccg gatgcggtgc aagcacctta tccggcctac agaccggagc caaacatgca
    28261 aaccatcacc atcgccccac ccaagcgtag ctggttctcg cttctgagct gggccgttgt
    28321 tctcgacgtg ctggtcgtct cgtggcaggg cgcggaaatg gccccgctta cgctgattaa
    28381 agacggcggc aacatggcaa cctt
//
LOCUS       DAJTSF010000050        28364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000050 DAJTSF010000000
VERSION     DAJTSF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.050"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="OO335_002243"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="OO335_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(1023..2045)
                     /locus_tag="OO335_002244"
     CDS             complement(1023..2045)
                     /locus_tag="OO335_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6002614.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /locus_tag="OO335_002245"
     CDS             complement(2059..3561)
                     /locus_tag="OO335_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6002615.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="OO335_002246"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="OO335_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="OO335_002247"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="OO335_002247"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6002616.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="OO335_002248"
     CDS             complement(5877..6218)
                     /locus_tag="OO335_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6002617.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="OO335_002249"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="OO335_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6002618.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="OO335_002250"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="OO335_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6002619.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="OO335_002251"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="OO335_002251"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6002620.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="OO335_002252"
     CDS             12897..14240
                     /locus_tag="OO335_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6002621.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="OO335_002253"
     CDS             complement(14302..14508)
                     /locus_tag="OO335_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6002622.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="OO335_002254"
     CDS             14833..15387
                     /locus_tag="OO335_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6002623.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..16388)
                     /locus_tag="OO335_002255"
     CDS             complement(15450..16388)
                     /locus_tag="OO335_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002624.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="OO335_002256"
     CDS             complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="OO335_002256"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6002625.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17530..19473
                     /gene="cpdB"
                     /locus_tag="OO335_002257"
     CDS             17530..19473
                     /gene="cpdB"
                     /locus_tag="OO335_002257"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6002626.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19599..19979)
                     /locus_tag="OO335_002258"
     CDS             complement(19599..19979)
                     /locus_tag="OO335_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6002627.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20068..20928
                     /gene="qorB"
                     /locus_tag="OO335_002259"
     CDS             20068..20928
                     /gene="qorB"
                     /locus_tag="OO335_002259"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6002628.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21037..22002
                     /locus_tag="OO335_002260"
     CDS             21037..22002
                     /locus_tag="OO335_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6002629.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22110..22772
                     /gene="ytfE"
                     /locus_tag="OO335_002261"
     CDS             22110..22772
                     /gene="ytfE"
                     /locus_tag="OO335_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6002630.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="OO335_002262"
                     /gene_synonym="dagA"
     CDS             complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="OO335_002262"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6002631.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="OO335_002263"
     CDS             complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="OO335_002263"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6002632.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25376..25921
                     /locus_tag="OO335_002264"
     CDS             25376..25921
                     /locus_tag="OO335_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR6002633.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25905..26552)
                     /locus_tag="OO335_002265"
     CDS             complement(25905..26552)
                     /locus_tag="OO335_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6002634.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26764..27555
                     /locus_tag="OO335_002266"
     CDS             26764..27555
                     /locus_tag="OO335_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR6002635.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27565..>28364
                     /locus_tag="OO335_002267"
     CDS             27565..>28364
                     /locus_tag="OO335_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR6002636.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6960 a         7550 c         6992 g         6862 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatcttttt tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt
    16321 gtatgcgatg actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg
    16381 ggtaacatat ggctacctta ttttatttgg ttattttatg tcgccattaa atatctcatt
    16441 atagaagtaa actatcaaaa aacgctcatt attaaaatgt aacttactag caattgaatt
    16501 tttgcacttt aaatacaatc tctttttatt tataactttt taggttggga taaatcagga
    16561 tggcagaatc aggaaataca ctgtttctgc catctgaatt tagtaaatgg acactctgaa
    16621 ccccggattc aggaacgact cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg
    16681 aacgtgcgct ccggcagctg cagccacagc atgtccggcg gcggtgtccc aaatattcgt
    16741 tggtccgaag cgcgggtaca gctgcgcctg tccttccgcc accaggcaga atttcagcga
    16801 agagccgatg gacgtggtct gatgttcgcc aagctgttgc agatactctt tcagctccgc
    16861 atctgcatgg gaacggctga taaccaccag cggcgggcgc gcatcgcgga cctgaatcag
    16921 cttgcgcaca ccgcactctt ctttccaggc cttgccttct gccgcgctgt acattacgtt
    16981 cattaccggc gcatacacca cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt
    17041 aacggtgaat tcgccattac gtttaataaa ctctttagta ccatccagcg ggtctaccag
    17101 ccagtaacgc tgccagtgct gacggacttc ccaaccggga ggatcttctt cagaaaggac
    17161 cggaatatcc ggtgccagcg tacgtaaacc gtccatgata acggtgtgag cggcaatatc
    17221 cgctgccgtt accggagaat tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc
    17281 gtagacctgc ataatggcat cgcctgcatt ccgtgcaagc tggcatactt gatctaacat
    17341 ttctccacct cgtctctgtg agcggtgtta acttattgtt ttacttatac cctatcgtta
    17401 atgaatgcgc caactgtgat agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc
    17461 actgttctat aagtaaggcg tttattctcc cttttctttc gtattcccga tgataaaagg
    17521 atgtccctga tgattaagtt tagcgcaacg ctcctggcca cgctgattgc cgccagtgtg
    17581 aatgcagcga cggtcgatct gcgtatcatg gaaaccactg atctgcatag caacatgatg
    17641 gatttcgatt attacaaaga caccgccacg gaaaaattcg gactggtacg tacggcaagc
    17701 ctgattaacg atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg
    17761 attcagggga gtccgctggc cgattacatg tcggcgaaag gattaaaagc aggtgatgtt
    17821 catccggttt ataaggcgct gaatacgctg gattatacgg tcggtacact cggcaatcat
    17881 gaatttaact acggtctgga ttacctgaaa aatgcgctgg cgggagcgaa attcccttat
    17941 gtaaatgcca acgtcattga cgccagaacc aaacagccaa tgtttacacc gtatttaatt
    18001 aaagataccg aagtggtcga taaagacgga aaaaaacaga cgctgaagat tggctatatt
    18061 ggcgtcgtgc cgccgcaaat catgggctgg gataaagcta atttatccgg aaaagtgacg
    18121 gtgaatgata ttaccgaaac cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc
    18181 gatgtcattg tcgttctggc gcattccggg ctgtctgccg atccgtataa agtaatggcg
    18241 gaaaactcag tttattacct cagtgaaatt ccgggcgtta acgccattat gtttggccat
    18301 gctcacgccg ttttcccggg taaagatttt gctgatatcg aaggggctga tatcaccaaa
    18361 ggcacgctga atggtgttcc ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg
    18421 gtcgacttac aactcagtaa tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa
    18481 gcacggccga tttacgacat cgccaataaa aaatccctcg cggcggaaga cagcaagctg
    18541 gtagaaacac tcaaagccga tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt
    18601 aaatctgccg acaatatgta tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa
    18661 gtcgtgaaca acgcgcaaaa agcgtatgtc gaacattaca ttcagggcga tccggatctg
    18721 gcaaaactgc cggtgctttc agctgccgca ccgtttaaag ttggtggtcg caaaaatgac
    18781 ccggcaagct atgtggaggt ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt
    18841 tatctctacc ccaatacgct gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg
    18901 ctggagtgct ccgccggaca gtttaaccag attgctccta acagcacgaa accacagtca
    18961 ctcatcaact gggatggttt ccgcacttat aactttgatg ttattgatgg tgtgaattat
    19021 cagattgatg ttacccaacc cgcccgctat gacggcgagt gccagatgat taatgccaat
    19081 gcagagcgga ttaagaacct gacctttaat ggcaagccga ttgatccgaa cgctatgttc
    19141 ctggttgcca ccaataacta tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc
    19201 catatcgctt ttgcttcacc ggatgagaac cgctcggtgc tggcagcgtg gattgctgat
    19261 gagtcgaaac gtgcggggga aattcatccg gcggcagata acaactggcg tttagcaccg
    19321 atagctgccg ataagaaact ggatatccgt ttcgaaactt ccccgtcaga taaagccgca
    19381 gcgtttatta aagagaaagg gcagtatccg atgaataaag tcgcgaccga tgatatcggg
    19441 tttgcgattt atcaggtgga tttgagtaag taaaacactt ctttttcggc ctataaatca
    19501 accgcatccg gcatttattg gcggatgcga tgctgccgta tcttatccac cctacaagcc
    19561 atgaaccgta gaccggataa gcgcagcgca tccggcagtt atgccgcacg ttcatcccgc
    19621 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    19681 ctcacctgct cgcccagcgg cgtgaggcta tattccacat gcggcggcac caccggatac
    19741 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    19801 tcactcaccc caccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    19861 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    19921 cactgttccg caaagagatt accctctttc agttgttgtg acaggctaac ctgactcatt
    19981 tcatacttac ctttttgcac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    20041 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    20101 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    20161 gttcgtaatc cggcaaaagc ccaggccctg gcagcacaag gcattaccgt gcgtcaggct
    20221 gactacagcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    20281 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    20341 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    20401 ctcggcctcg ccgatgagca catcgagacg gagaaaatgc tggctgattc tggcatcgtt
    20461 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    20521 ctggaacacg gcgtatttat cggtgcggct ggcgatggca aaatcgcctc agcaacgcgg
    20581 gcagattatg cgacagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    20641 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    20701 cagagtggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    20761 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    20821 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ctgcccaacg
    20881 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    20941 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    21001 tgaacgtttc ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc
    21061 ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt gccggaatac
    21121 ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctacccatt
    21181 gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg
    21241 atgctcacta tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt
    21301 actggcgcgc ctgtttccac gatgattatc ggcaccctgc cggtggtgat tcctgtcttt
    21361 gccaatctgc tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca
    21421 ctgatttgta ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc
    21481 cccgattttg actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc
    21541 tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat
    21601 ccgatgatgt gggcgacggc gcaggcgctg gtcacgctgc cggtttctct catcggctat
    21661 ctcgtcgcct gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc
    21721 cgtccgctgg tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc
    21781 gcactctgct ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt
    21841 gttttcgaaa cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg
    21901 ctaatgacgc tgagcggtat cgcgctgtta gtgattggcg tggtcatcgc ggtcagagca
    21961 aaaccagaaa aacctttaac tgaatctgtc tcagaaagtt gacatgctgg cagtgagtta
    22021 aataagcctc tgctacgtaa gggttatagc ttttgcctta aaggtgtatt taaaatacat
    22081 cttatcttat taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac
    22141 tggcgctctc tatccctcgc gcttcggctc tgtttcgtaa atatgatatg gattactgct
    22201 gtggcggtaa gcagacgctg gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca
    22261 ttgaagctga actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc
    22321 cgctggcaga aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcagc
    22381 tgccggagct gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg
    22441 tgccaaaagg gctgacaaaa tacctgacca tgctgcatga agagctttcc agccacatga
    22501 tgaaagaaga gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg
    22561 ggccaatcag cgtaatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatca
    22621 aacacactac caataacgtc acaccgccgc cagaagcctg caccacctgg aaagcgatgt
    22681 ataacggcat taatgaactg attgatgacc tgatggatca catcagtctg gaaaacaatg
    22741 tactgttccc acgcgcccta gcgggtgagt gatctaaaag ctggatggca ttgcgccatc
    22801 cagcatgata atgcggttat ttccgcagtt cagcagcccg cttcttacca ataaacagcc
    22861 agcccaaccc cagcgcgata aaccacagcg gggtaaccag cagcgcctgg cgagtgtcat
    22921 cttccagtgt cagcaacacg accacgaaca caaagaacgc catacatacc cagcacatca
    22981 gcttgccgag cggcatcttg tagatcgact tttcatgcag atgaggacgc tgtttgcggt
    23041 acaccaggta agagcaaagg ataatcgtcc agacgaacat aaacagaatc gcggaaacgg
    23101 ttgtaatcat cgtgaacgcg ccaatcacgc taggattcac atacaacatc accacgccgc
    23161 cgagcagaca gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt
    23221 tagcgaacgc tttcggcgca acaccttcct gcgccagacc aaacagcata cggctggtag
    23281 agaagacacc gctgttagcg gaagacgccg cagaggtcag caccacaaag ttgatcacgc
    23341 tggcagccgc aggcaaacct accaacacaa acagctcaac gaacgggctt ttctccggga
    23401 ctaccgaact ccacggtgtc acggacataa tcacaatcag cgcgaagacg tagaacataa
    23461 tgatacggat cggaatggag ttaatcgcgc gtggcagtga tttctccgga tctttggttt
    23521 ccgcagctgt tgtacctacc agctcaatcc ccacgaaagc gaaaaccgct atctggaatc
    23581 cggcaaagaa gccacttaaa cctttcggga accagccgcc gtcattccac aaatgcgcga
    23641 atgacgcttc cacaccggtc ggtgactgaa agtgcatcgc caccatgacc aggccgacga
    23701 caatcagcga cacgatggcg acgattttga tcatcgcaaa ccagaactcc atctcaccga
    23761 acattttcac ggtggcaagg ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga
    23821 cccagtcgga gagatcgggg aaccagaact gagcataagc cgtgatcgcc accacgtctg
    23881 ccataccggt tacaacccag cagaaccagt aagtccagcc ggtgaaatat cccgcccacg
    23941 gccccagtaa atcggaagcg aagtcactaa aagatttgta ttccagattc gaaagcagca
    24001 attcccccat tgcccgcatc acgaaaaaga gcataaaacc aatgatcata taaacgaaaa
    24061 tgatcgacgg cccggcaagg ctaatcgttt tgccggaccc cataaacaac ccagtaccaa
    24121 tggcacctcc aatggcaata agctgaacat gtcggtttgt gagattgcgc cgtagcgact
    24181 gttcagccgg agcctgatca tcggcaacga cttttacctg atctaccatg ttttttcttc
    24241 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    24301 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    24361 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    24421 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    24481 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    24541 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcacct
    24601 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    24661 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    24721 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    24781 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    24841 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    24901 ttcacgcctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    24961 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    25021 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    25081 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    25141 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    25201 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    25261 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    25321 gttagcttat tcataggcta tgattgagga acaagacgtg gagcaggagg aaaccatgcc
    25381 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    25441 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    25501 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgtcggtgg tgacgcgtga
    25561 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc cgcaaactac
    25621 ccaaacgcaa ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt
    25681 cgaacccggt aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt
    25741 ctatgcaatg gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca
    25801 gatggtgaaa atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg
    25861 gaataatcaa caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta
    25921 gatgaagggt caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct
    25981 tccagtgcct ggggggaaac catactgata tgataaacta ggccaagcgt atgctggttg
    26041 atcaaataat atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatacct
    26101 ggcttaaaaa tattgtattt cttcccttca tccaataagg attccattaa ctgcaagtga
    26161 gcatggttaa tttctaagag acgctgtgat tttgcataat gcacaccttt actttgattt
    26221 tcactatgca caatttttaa aaaccaagga ttagcaatat aataatccca tgtaaaatca
    26281 ataattttct caatggcttt ttgtggcgga agatctgaga aatcaagggc cttttctttt
    26341 tgtcgaatat cgttccaggt atattccagc acttcgataa ataaattttc tttattacca
    26401 aagtggtgat aaaccgtctg tttactacac cctgctgcct ttacaatatt atctactctg
    26461 gccccttcat aaccatattc ggcaaattca ttaaccgcac atagcagcaa tttttccttc
    26521 aggctctgta cgttgctggt atcaatatac ataacgcttc cccacgacct cttcgtggtg
    26581 actatcataa cctgatttga tttcagcttc agaaaaaagg gcaaaaactt gtactttcat
    26641 caggttatca aatgtcctgt gatcgccatc gcatatcaac caactagatg gtttatcttt
    26701 ttatcctcca gtcgcaagct aatcatcacc aaggaaacaa aattcaaaaa agagagctat
    26761 tgtatgaaat ccactcgtcc tgttgccgtg attaccggag ccactcgcgg tattggaaag
    26821 ggctgtgcgc aggagcttgc tcgtggcggg tttaacctgc tgattaacga tcgtcccgat
    26881 gccgacagcg tagaaaaact gcatatcaca caacaagaat gtttggcgga aggcgtggaa
    26941 gtgatttgct tccctgctga tgtcggtgat ctatctctgc atgaggagat gctcgacgcg
    27001 gcgcaaaacc agtgggggcg gctggactgt ctgctcaata acgcggggat ttcagtgaaa
    27061 aaacgcggtg acctcctcga cctcgaacca gacagcttcg atcaaaacat tgccattaac
    27121 acccgcgcgc cgttcttcct tgctcaggca ttcagtaagc gcttactggc acagccgaaa
    27181 ccggaagcgg agctgccgca ccgctcgatt attttcgtca gttccatcaa cgccatcatg
    27241 ctggcgatga atcgcggcga atacaccatt gccaaaaccg ccgtttctgc cgctgctcgt
    27301 ctgtttgctg ctcgcctttg taatgaacaa attggcgttt atgaagtgcg ccctggtctc
    27361 atcaaaactg atatgactat tccggcaacg gcttattacg acgaactgat tgccaaaggt
    27421 ctggtgccgt ggggacgttg gggttatccg gctgatattg cttccaccgt ccgcgcgatg
    27481 gcggaaggta aactgattta cacctgtggt caggcggtcg caatcgacgg cggcttaagt
    27541 atgccgcgct tctgaggctg aatcatgaga gatctgttac aacgcccgga tttattcagt
    27601 atcaataccg cgacactggg ctataaaacg ccgctacccg ccattattga tgcctgcgcc
    27661 gcacgcggta ttggcgcaat tgccccctgg cgcagggagt tgcagggtga agatctccag
    27721 caaatcaccc gccagcttgc cgccagtaac atgagcgttt ccggtttgtg ccgtagcact
    27781 tactacaccg cgccgacact ggcggaacgc aaactggcga ttgatgataa ccgccgggcg
    27841 ctggacgacg cagcggtgct taacgccgcc tgttatatgc aggtggttgg cggtctgcca
    27901 acgggcacta aagatctata tgaagcgcga gaacaagtga aacaagggat tcgccagttg
    27961 ctgccacatt cgaaagatgt cggcgtgcca attgcgctgg agccactgca cccgatgaca
    28021 gctgccgatc gctcttgcct gtgcacgtta cgtcaggcgc tggactggtg tgatgaactc
    28081 gatcccgatg gtgagtttgg cctcggcgtg gcggtggatg tttatcacgt ctggtgggat
    28141 ccggatctcg ccagtcagat cctgcgtgcc ggaaaacgca tcctcgcgtt tcatgtttcc
    28201 gactggttaa tgccgaccac tgatctggtc aatgaccgtg gtatgccggg agatggcgtc
    28261 atcaatattc cgtcaattcg tcggctggtt gaaaacgccg gatttaatgg tgctattgaa
    28321 ctggaaatat tctcgcctta ctggtggcag aaagatatta acag
//
LOCUS       DAJTSF010000051        27530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000051 DAJTSF010000000
VERSION     DAJTSF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.051"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            248..1546
                     /gene="kgtP"
                     /locus_tag="OO335_002268"
     CDS             248..1546
                     /gene="kgtP"
                     /locus_tag="OO335_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6002637.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1543..1866)
                     /locus_tag="OO335_002269"
     CDS             complement(1543..1866)
                     /locus_tag="OO335_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6002638.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1912..3267)
                     /gene="pssA"
                     /locus_tag="OO335_002270"
     CDS             complement(1912..3267)
                     /gene="pssA"
                     /locus_tag="OO335_002270"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6002639.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3381..6041)
                     /gene="pat"
                     /locus_tag="OO335_002271"
     CDS             complement(3381..6041)
                     /gene="pat"
                     /locus_tag="OO335_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6002640.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6073..6771)
                     /gene="tapT"
                     /locus_tag="OO335_002272"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6073..6771)
                     /gene="tapT"
                     /locus_tag="OO335_002272"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6002641.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6840..7259)
                     /gene="trxC"
                     /locus_tag="OO335_002273"
     CDS             complement(6840..7259)
                     /gene="trxC"
                     /locus_tag="OO335_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6002642.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7466..8503
                     /locus_tag="OO335_002274"
     CDS             7466..8503
                     /locus_tag="OO335_002274"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6002643.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8551..9240)
                     /gene="ung"
                     /locus_tag="OO335_002275"
     CDS             complement(8551..9240)
                     /gene="ung"
                     /locus_tag="OO335_002275"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6002644.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9545..9928
                     /gene="grcA"
                     /locus_tag="OO335_002276"
     CDS             9545..9928
                     /gene="grcA"
                     /locus_tag="OO335_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6002645.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(9984..10571)
                     /gene="eamB"
                     /locus_tag="OO335_002277"
     CDS             complement(9984..10571)
                     /gene="eamB"
                     /locus_tag="OO335_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6002646.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10674..11555
                     /locus_tag="OO335_002278"
     CDS             10674..11555
                     /locus_tag="OO335_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6002647.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11590..12924)
                     /gene="srmB"
                     /locus_tag="OO335_002279"
     CDS             complement(11590..12924)
                     /gene="srmB"
                     /locus_tag="OO335_002279"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6002648.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13056..13793
                     /gene="trmN"
                     /locus_tag="OO335_002280"
     CDS             13056..13793
                     /gene="trmN"
                     /locus_tag="OO335_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6002649.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13778..15400)
                     /gene="nadB"
                     /locus_tag="OO335_002281"
                     /pseudo
     CDS             complement(13778..15400)
                     /gene="nadB"
                     /locus_tag="OO335_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15656..15811
                     /gene="rseD"
                     /locus_tag="OO335_002282"
     CDS             15656..15811
                     /gene="rseD"
                     /locus_tag="OO335_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6002650.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15808..16383
                     /gene="rpoE"
                     /locus_tag="OO335_002283"
     CDS             15808..16383
                     /gene="rpoE"
                     /locus_tag="OO335_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6002651.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16416..17066
                     /gene="rseA"
                     /locus_tag="OO335_002284"
     CDS             16416..17066
                     /gene="rseA"
                     /locus_tag="OO335_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6002652.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17066..18022
                     /gene="rseB"
                     /locus_tag="OO335_002285"
     CDS             17066..18022
                     /gene="rseB"
                     /locus_tag="OO335_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6002653.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18019..18498
                     /gene="rseC"
                     /locus_tag="OO335_002286"
     CDS             18019..18498
                     /gene="rseC"
                     /locus_tag="OO335_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6002654.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18696..20495
                     /gene="lepA"
                     /locus_tag="OO335_002287"
     CDS             18696..20495
                     /gene="lepA"
                     /locus_tag="OO335_002287"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6002655.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20511..21485
                     /gene="lepB"
                     /locus_tag="OO335_002288"
     CDS             20511..21485
                     /gene="lepB"
                     /locus_tag="OO335_002288"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6002656.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21757..22437
                     /gene="rnc"
                     /locus_tag="OO335_002289"
     CDS             21757..22437
                     /gene="rnc"
                     /locus_tag="OO335_002289"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6002657.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22434..23339
                     /gene="era"
                     /locus_tag="OO335_002290"
     CDS             22434..23339
                     /gene="era"
                     /locus_tag="OO335_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6002658.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGSKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23351..24079
                     /gene="recO"
                     /locus_tag="OO335_002291"
     CDS             23351..24079
                     /gene="recO"
                     /locus_tag="OO335_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6002659.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24091..24822
                     /gene="pdxJ"
                     /locus_tag="OO335_002292"
     CDS             24091..24822
                     /gene="pdxJ"
                     /locus_tag="OO335_002292"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6002660.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24822..25202
                     /locus_tag="OO335_002293"
     CDS             24822..25202
                     /locus_tag="OO335_002293"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6002661.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25622..25702
                     /gene="shoB"
                     /locus_tag="OO335_002294"
     CDS             25622..25702
                     /gene="shoB"
                     /locus_tag="OO335_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6002662.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25896..26156)
                     /gene="yfhL"
                     /locus_tag="OO335_002295"
     CDS             complement(25896..26156)
                     /gene="yfhL"
                     /locus_tag="OO335_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6002663.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26371..27467
                     /locus_tag="OO335_002296"
                     /pseudo
     CDS             26371..27467
                     /locus_tag="OO335_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6788 a         6848 c         7216 g         6678 t
ORIGIN      
        1 gcgtcttata ctcccacata tgacagattc agcaacggat gcggtttccc caacttgcct
       61 acttccatac gtgtcctcct taccagaaat ttatccttaa gctcctcaat aaccattttc
      121 ctgctaacta aattcatggt ttaggttgca taatgatacg caacaaatgt ataacatttt
      181 ctttaccaaa agaaataaac aaaagcgacc aacaaaagca tcggattacg gcaggagaca
      241 taatggcatg gctgaaagta ctgtaacggc agacagcaaa ctgacaagta gtgatactcg
      301 tcgccgcatt tgggcgattg tgggggcctc ttcaggtaat ctggtcgagt ggttcgattt
      361 ctatgtctac tcgttctgtt cactctactt cgcccacatt ttcttccctt ccgggaacac
      421 gacgactcaa ctactacaaa cagcaggtgt ttttgctgcg ggattcctga tgcgcccaat
      481 aggcggttgg ctatttggcc gcatagccga taaacatggt cgcaaaaaat cgatgctgtt
      541 atcggtgtgt atgatgtgtt tcggatcgct ggttatcgcc tgcctcccag gttatgaaac
      601 tataggtacg tgggctccgg cattattgct tctcgctcgt ttatttcagg gattatccgt
      661 tggcggagaa tatggcacca gcgccaccta tatgagtgaa gttgccgttg aagggcgcaa
      721 aggtttttac gcatcatttc agtatgtgac gttgatcggc ggacaactgc tagccctact
      781 ggttgtcgtg gttttacaac acaccatgga agacgctgca ctcagagagt ggggatggcg
      841 tattcctttc gcgttaggag ctgtgttagc tgttgtggcg ttgtggttac gtcgtcaatt
      901 agatgaaact tcgcaacaag aaacgcgcgc tttaaaagaa gctggatctc tgaaaggatt
      961 atggcgcaat cgccgtgcat tcatcatggt tctcggtttt accgctgcgg gctccctttg
     1021 tttctatacc ttcaccactt atatgcagaa gtatctggta aatactgcgg gaatgcatgc
     1081 caacgtggcg agtggcatta tgactgccgc attgtttgta ttcatgctta ttcaaccact
     1141 cattggcgcg ctgtcggata agattggtcg ccgtacctca atgttatgtt tcggttcgct
     1201 ggcagccatt tttaccgttc ctattctctc agcattgcaa aacgtttcct cgccttatgc
     1261 cgcttttggt ctggtgatgt gtgccctgct gatagtgagt ttttatacat caatcagtgg
     1321 aatactgaag gctgagatgt tcccagcaca ggttcgcgca ttaggcgttg gtctgtcata
     1381 tgcggtcgct aatgctatat ttggtggttc ggcggagtac gtagcgttgt cgctgaaatc
     1441 aataggaatg gaaacagcct tcttctggta tgtgaccttg atggccgtgg tggcgtttct
     1501 ggtttctttg atgctacatc gcaaagggaa ggggatgcgt ctttagtgac gggtcagttg
     1561 ccagacggta tagccggtgc ttgcaccggc gacatcccag gccaaatcct tccagctcca
     1621 gccgctccct tcggggcggc tatcccaaag ctcttttgac gcccccaaac tgacagagaa
     1681 catcaatcca aacatggcac tgcgatcccg gctcatcccc tgatgctgtg aatattcatt
     1741 tccggcggcg gaaagtatcg ccgaggcgat aaagtgttga gctttatcct gcccactcca
     1801 gctatcgtta tccatatggc tgcattcaga aagtaacaat agcgaacaga caaaaagaat
     1861 acgcaaaacg tggcctccaa aaaacaaacc ccgtacagga cggggttgtg attacaggat
     1921 gcggctaatt aatcggtcga tgcggatacg gcgcaaacgg cggatgagtt tacgaacctt
     1981 caccggataa tcggcaatac tttgcagatc gcgatagtgc ttaacgatgg tggtacgctc
     2041 gcggatcagc tccagttctt tctctcgctg cggcgcaagc tccagttgtg gatcgtggat
     2101 caaaatggcg ttttccagat ccagacgcca ggcgcgcggg ttcaggttat taccggtgat
     2161 caacatccac ttatcatcaa cccacatccc tttcaggtga taggtgttgt cgtcatcttt
     2221 ccataaccga accactagct ggtcagtatt gacgtaatac tgcaaacggc tcaggaaacg
     2281 acgcagattg atctcataga gataaggcaa tgcgccaatt atcttgaaag gttcatcttc
     2341 cggaatgtag aagtcgttcg cggttttatc accaacaata atttcgacct ttttcccttc
     2401 gcgcagcaac tggataatat tgcgcacaag gattgctggc aggttgaagt atggcgtaca
     2461 gatggttagt ttctgctcgg cacaaggcat aagatggaaa atggtcttgt tcaacagact
     2521 cgatttcccc agccccacta gcggcgttac ggaaagctga tcgttgtcgg catcgccctg
     2581 gaaatgataa gcggcatcac gcagctcctg gcggaacaga cgaatatcgt tcttgatttc
     2641 cgggcttttt ggccgattaa catcatccag acgattaacg ccgcggccat tcataatatt
     2701 ctgtgtaacc cattcaaaca taatgtctga catcttacgg ttacggatca gatgataacg
     2761 gtcgtagcga tatttatcgt gttgatgcag gtaaacatcg ttcaggctgg caccgctata
     2821 aagtacgcta tcgtcgatga taaagccttt aaagtgcaga acaccaagag cttcacgggt
     2881 attgattgga acgccataaa ccggaacatc tacgcccgga ttttcctgcg ccatgcggca
     2941 gtaccagtca gcgttagtgt tagatgccgc agcgccaatg cgtccacgtt gtgcacgatg
     3001 ccagtcgacc agcacccgca catccagttc cggacgctgc cttttagcct catacaacgc
     3061 gttcagaatg cctttgccac cgtcatcctg ttcgagatac agggcgacaa tgcaaatgcg
     3121 ctgcttcgcg ctggctattt tttccagcag cgtctcccgg aagtcggcgg gagcgtaaaa
     3181 gaaatcgaca tcatcaactg attgagaaat cttgggtagt tgggcaaggt gttgttgatg
     3241 tttattacgc ttaaattttg acaacatcac agtgcatttc ttctctgttc attgaagggt
     3301 cctctgtgca atgcagacga cataagcggg caataataac accagtcccg attaagtggt
     3361 caacatttcc agtaccttac tcatgattcc tcgcgccggg caagatttag cgtaagccca
     3421 acgatcccct cttcgagctg gatatcaacg ttaaacccga gcttgcgggc tagcgccacc
     3481 atgccacgat tgtttggcat cgtaatacca ttcagacgtt gtagtccgtg atttcgcgta
     3541 taggtaatca acttttccat taagcgtcga cctaagccta accctttgag atccgagcga
     3601 accagtacag caaattcggc atcgatgtta tcaggatcgg aaatcgcacg cgtgacgccg
     3661 aggatctctt ccgtttgatc aatacgtcgt accgctacaa acgccatttc ccgatcgtag
     3721 tcgatctgtg tcatgttggc taaatcttca tgggtaaatt cgttgatctc gctaaagtag
     3781 cggtaataaa gatcttcttt ggtgactcgc gaaatgaatt gctgaagttg tggctcatct
     3841 tctggcaaaa tcgggcggaa caagcagcgt tcaccgtttt tcaattctac ccattcttcc
     3901 agctgatgcg gataagggcg cactgccagc cgactctcgt tatcgccttc aaacggcgag
     3961 atatccagcg tgacatccag cgcggtaaat tcactgccag aagccagcaa aggatgaata
     4021 tccagacgct gaatttccgg gcaatcgaca atcaagttgg aaacctgcac cagaagctgg
     4081 ctcaagcctg caacatccaa tgggcgtagc gcactgcgcg cacgaatctt tttacttttg
     4141 atcccctgaa taaccagata gcgggccagg ttcatgttca gcggcggcag tgcgacgacg
     4201 gcttgatctt caggacgcca ctccacaccg ccttcaccca gcatgatcaa cggcccgaaa
     4261 accggatcgt gctcaaccac aacccgcaac tcctgagcgc cagcacggtt agccatactt
     4321 tgcaccaaca ggccgtggac ccgcgcctgt ggccaggcca tttttacgcg atcgaaaata
     4381 gcgttcgccg cttgctggac ttcattggct gtacgcaggt aaagcatgac gccctgaact
     4441 tccgatttat gtggaatatc cggcgaacgc aatttcagcg ccaccggata accaatctgt
     4501 tcggcaatat gcaccgcttc ggtgctatcg ctggcaatcc aggttgggag cgtgttcatg
     4561 ccatacgctt gcaggatggg ctgaacttca tgggtatcga gcgacgtagc cccttcggca
     4621 atcgcctgtt gcaacagaag atgcgcttct gcggtattgg aagtcagatt gctgggcaac
     4681 gccggcgttt cgcgtagttg cttctgatta cgccggtact ccaccatatg cataaaagca
     4741 gtgatggttc cttccggggt acggtaggtc ggcagcccgg cttcgctgaa taaacgtcgt
     4801 gcctcttgcg aggagtgctc gccgcaccag ttcgtcagca aagagacata tttgctgcgg
     4861 ggatgatgct ttaccgcttc aattaatact tgcgcgcttt ctgttgcggg agcagcggcg
     4921 ctgggcgaat gaataaccat cagtgcatca aaatcctggc tgtggagcag aatgtccagc
     4981 gttttgacat aatgctcgct gctggcgtca tcgcgcagat cgagcgggtt agagactgcc
     5041 acatgttctg gcaatgcatc gcgcaatttc tggcaggttt cttcgcttag cgttgccagc
     5101 ttgccattgc gtgaccataa ggcatccagc gccagcgcgg caggcgcagc accgttgctg
     5161 ataatcatca accggtcgcc acgcagcggg cgcatatggc taagggtttc caccgccgaa
     5221 aacagttcgt gggtatcctg tacccgcaac aaaccggcac gctgaatagc cgcatcccat
     5281 gccgggtcca ttcctgccgt ggtgttgagc agtcgctgtg ccgccgggct acgtccgctt
     5341 ttaatcacca gaatcggttt attacgcgag gcactacggg ccgccgaaac aaagcgtcgc
     5401 gcgtcgctta actgttcgag atagagcagg atggcgctgg ttttactgtc gcgcgccaga
     5461 tagtcgagca attcatcaac gtcgatatct aggctgtcgc cgagcgcaat aaagtaggaa
     5521 aagcccatct cacgctgttg cgcccagtcg aggatggtgt tggagacggc agccgattgc
     5581 gaaataaacg ccagcttgcc gcgtttaatc ggcacaggcg aaaagctggc attcagacct
     5641 tgccagggag ccagtaaacc cagactgttt ggtccaagca ggcgcatgtt atggcgcagg
     5701 gcgcaggcgc ggagatcttc gtgttgcgat gccggggcgg aaagaataat gcaggtttta
     5761 cagcctttct cgccgagctc ttccagaaga gcaagattac ggctggcatt ggtacataaa
     5821 accgcaaggt cgggtgtaaa gggcaagctg gcaatatccg gccaggccaa cacacccaac
     5881 accgctttcc aggctggcgt caccgggagt accggtccgt taaagcctcc cgccagcagg
     5941 ttacgcatca tcaggtaacc tgcgcgattg ggtttcatcg acgcgccaat taccgctatc
     6001 gattttggtc gcagtagtgc ttccagtcct cgctgactca taccggtctc ccgcagaagt
     6061 gaccgaatga ttttaaacgc tttctaactg ttctgctgtg atgctaccca gatgttgcgt
     6121 ttttcctgcc agatagcgtg ttttaaagcg ggtaaaatgc tcgcctaacc ctgctgccgc
     6181 cccggtatcg ccggccatat ctaacagtgc gatggctacc tcggcagtac aatattggcc
     6241 tgcagcctgg gcttcacgca ggcgataggc agaaagccgg gaaagatcga cggaaatgac
     6301 gggaagatta tccagatacg gacttttacg aaacatcttg cgagcttccg gccaggtacc
     6361 atcgagcatg ataaacagcg gtggcttacc ggcaggtggt gtgaagatca cttcccgttg
     6421 ctcatcagca tacgaggcgg gaaagaccac cattggctga taatccgggt tttgtaccag
     6481 atccagcaaa tcctgcgagg gttcggtacg cgaccattga aacgcaacgg tatcaggcaa
     6541 aatatcagca atcagacgcc cggtattact gggcttcatt ggctcggtgt cgaacatcag
     6601 caaacagaag cgactttttg cttgtgctgg ggtaattgtc gaacagagac ataatttctc
     6661 tggcaaaaga cagcgttggc agcgacgaac gcgattaccg cgggcaagaa aaggacgtgt
     6721 tgcgcgcgca atacgctcgg cgcgtaactg gagaacagcg ttttcggtca taagagagcg
     6781 tcgaaaaaac gccattgtcg cagaggagaa aacggggcac aagatgcgcc ccggtaagat
     6841 taaagagatt cgttcagcca gctatcgaac ggcgctttcg gtactgcgcc attaagcatg
     6901 tcgacaacct gaccgttttt gaaaatcatg atcgtcggta tactacgaat tccaaagcga
     6961 ctgctcaatt cacgttcagc ttcggtattc actttcacaa agcgcacttt accgctacgc
     7021 tcttgcgcga catcttcaaa aattggtgcg aaattacggc aggggccgca ccacggtgcc
     7081 cagaagtcga tcaccacagg tagatcatcc ttcagcaatt tgtcgagtgt ttcaccggtc
     7141 gcattaatca cctctccgtc aaacaagtcg tgaccgcagc gtccgcattt tgccgcatct
     7201 tcgatccgat cgtcgggaat gcgattgatg gcctgacaat gggtacaaac ggtattcata
     7261 actaacctcg ggatgagtaa ggagacttat cggcaacgac gccggtatgt ttctaatatg
     7321 ttacatatta tcggggacat tgttccagac aacaatctgt tttattcaat aggtatgata
     7381 gtcgcaggct ttgctacgaa atcatggcga accgcgcgga cttcgggtaa tctgcgcgct
     7441 tcgcgcagcg ctggtggaga aaagcatgaa cgatgaaatg aaaggtaaaa gcggcaaggt
     7501 caaagtgatg tatgtccgca gtgatgatga ttctgataaa cgtacccaca acccgcgtac
     7561 cgggaaaggg ggcgggcgtc caggaaaatc tcgtgctgat ggtggccgtc gccccgcccg
     7621 cgatgacaaa cagagtcagc cccgtgaccg caagtgggaa gattcgccgt ggcgcacggt
     7681 ttcccgcgcg ccgggtgatg agacgccgga aaaggccgat cacggtggca tcagtggtaa
     7741 aagttttatt gatccggaag tgttgcgtcg tcagcgtgcg gaagaaaccc gcgtctacgg
     7801 cgaaaacgcc tgtcaggcac tgttccagag ccgtccggaa gcgattgttc gcgcctggtt
     7861 tatccagagt gtaacgccgc gttttaaaga agccttgcgc tggatggcag caaaccgcaa
     7921 agcgtaccat gtggtggatg aagcggaatt gacaaaagcg tcaggtacgg aacatcacgg
     7981 cggcgtttgc ttcctgatca aaaagcgtaa cggtacaacc gtgcagcagt gggtaatcca
     8041 ggcaggcgcg caggattgcg ttctggcact ggaaaacgaa tctaacccgc ataacctggg
     8101 tggcatgatg cgcagctgcg cgcacttcgg cgtgaaaggt gtcgtggtgc aggatgcggc
     8161 actgctggag tcgggggcgg ctatccgtac cgcagaaggc ggcgcagagc acgttcagcc
     8221 gattactggc gacaacattg ttaacgtgct ggatgatttc cgtcaggcag gttacaccgt
     8281 agtgaccact tccagcgagc agggtaaacc gctgttcaaa accagtctgc cagcgaaaat
     8341 ggtactggtg ctgggtcagg aatatgaagg gttaccggat gccgcacgcg atccgaacga
     8401 tctgcgcgtg aagattgacg gtacgggcaa cgttgccggg ctgaatatct ctgtcgcaac
     8461 cggcgttttg cttggtgaat ggtggcgtca gaataaagcc tgataaatca gccgggtggc
     8521 aactctgcca tccggcattt ccccgcaaat ttactcacac tctgccggta atactggcat
     8581 ccagtcaatc ggcgtctcgc cacgttgttc cagccactga tttgccagca caaaatggtt
     8641 gcagccaaag aatccacgat gcgccgaaag cggcgacgga tgcggtgctt tcagtacatg
     8701 atggcgttgc ttatctataa tcgccccttt cttttgcgca tgtgatcccc acaacaaaaa
     8761 caccacgcct tcgcggtgct ggttaatcag gctgatgact ttatcagtaa acgtctccca
     8821 accgaggctg gcgtgggaat gcgcctgacc tgcgcgaacc gtcaacacag tattgagcag
     8881 cagaacgccc tgacgcgccc agctttcgag atagccatga ttagggcggg tgaagcccgg
     8941 aatagtattt tccagctctt tatacatatt caataatgac ggaggaatgg caatgccggg
     9001 acgaacggaa aatgccagac catgcgcctg tcccggtccg tgataaggat cctggccgag
     9061 aatcaccact ttaacgtcac ccaactcggt aaagcggaac gcgttaaaga catctttttg
     9121 tggtggatag atagtgacgc cggactgccg ctcgctggcg acggtctgaa gggtattaag
     9181 aaaataaggt tgttgcttct cttcagccag cacgtcatgc caggttaatt cgttagccat
     9241 cttgctctcc tgcgaatctt caatccgcct agcttaactg cttctttcta cagaacaaaa
     9301 tcatgctcat tttttagact taaacgagtg caaaaaaatg taaattggta aaatttcaca
     9361 cccgcataag ttgatgtaaa acaataaaat ccatttacga ccacaatcga tgtgtggttt
     9421 ttgttgattt aaatcaaaga atcaagggtg gttgaggagt atatatacac tcaagcaaca
     9481 atggttttac caattggccg cgacaggctg aacaaatcaa ataattttgc cggggaggca
     9541 tcacatgatt acaggtatcc agattactaa agccgctaac gccgatctgc tgaactcttt
     9601 ctggctgctg gacagcgaaa aaggcgaagc acgttgcatc gttgcaaaag caggttatgc
     9661 agaagatgaa gtggttgcag taagcaaact gggtgacatt gaataccgtg aagttccagt
     9721 agaagtgaaa ccagaagttc gcgttgaagg tggtcaacac ctgaacgtta acgttctgcg
     9781 tcgtgaaact ctggaagatg cagttaagca tccggaaaaa tatccgcagc tgaccatccg
     9841 tgtatccggt tatgcagttc gctttaactc tctgactccg gaacagcagc gcgacgttat
     9901 cgctcgtacc tttactgaaa gcctgtaagg cttaagtagc aagttactaa gcggaagagg
     9961 gcgacctctt ccgctttttt tcgttaatag aaaatgcgta ccgcgcaata gaccagcaac
    10021 agggcaagca cgatatttaa ctggcgacca tactggcgaa acaatcgctg aaacagatgc
    10081 cccgccagcg cccagcacac attgccaaac gtcccaatca tcgccagcaa aacgctgacg
    10141 ccaactaccc agcttagcgc ctgtgtttgc ggcagaacaa acgtcgacag tgccgtaaca
    10201 ccgtacaaaa tgattttgac gttcacaaac tgcaaagcaa agctggccca aaagctgatt
    10261 ggttttgcct gaagtccgtc ttcctttgtt gggctggtgg cgattttcca cgccagccag
    10321 acaatatatg ccgcccccgc ccaactcaaa aggtgtaccg ctgccgggtc aatcactgcc
    10381 agtgaaaatg aaatgcccgc acacagtaac atcacaatca aaaatcccag actcatccct
    10441 gccagcacgc gggtactttg acgaaatcca tgcgacgtag cagagctaag ggcgagaata
    10501 ttgttcggtc ctggcgtcat agcggtaatc agggtgtaag tccaaaaagc acttaaaagg
    10561 atcggtgtca cttgttatct cctctgtttt ggtagacagt acccgacacg gaacatttaa
    10621 aaaaatgaat gtttttatat tctaaattcg atattttcga taggtttggg gttatggatc
    10681 tgcgccgttt tattacgctt aaaaccgtgg tggaagaggg ttcctttttg cgagcttcgc
    10741 aaaaattgtg ctgtacacaa tcgacggtga cttttcatat tcagcagctt gagcaggagt
    10801 tttcagtcca gttatttgag aaaattggcc gacggatgtg ccttacccgc gaaggaaaaa
    10861 agttgctgcc gcacatttat gaacttaccc gagtgatgga tacgcttcgt gaggctgcca
    10921 aaaaggagtc agatccggac ggggaactac gcgttgtttc gggcgaaacg ttgctctctt
    10981 accgaatgcc gcaggtgttg cagcgttttc ggcaacgcgc accaaaagtg cgtttatcgt
    11041 tgcaggcgct gaactgttac gtgatccgtg atgcgctact gaatgatgag gccgatgtcg
    11101 gcgtctttta tcgcgtaggg aatgatgatg ccctgaaccg acgagagttg ggtgaacaat
    11161 cgctggtgct ggtggcttca ccacaaattg cagatgtcga ttttactgag ccgggaagac
    11221 ataacgcctg tagctttatt atcaacgaac cgcaatgtgt cttccggcag atatttgaga
    11281 gcacgctgcg acagcggcgg atcacggtgg aaaacaccat tgagcttata agtatcgaaa
    11341 gcatcaagcg ttgtgtcgcg gcgaatatcg gtgtcagtta cttgccccac tttgcggtgg
    11401 aaaaagagat tgagagcgga gaattaattg aattgccttt tggcgaacag tcgcagacca
    11461 ttacggcaat gtgcgcacat tatgctggaa aagcggttag cccggcgatg cacattttta
    11521 ttcagtgtgt tgaagagtgt tttgtggttg cgtaaaaaaa caatgccggg cttaaacccg
    11581 gcattgagat tactcttctg tcgtttgcgg tggcacgccc gttccgctcg gtttacggcg
    11641 cttaccaata tttttggtgt cgcgatggcg ttttttcacc cgcggcttct ctttctcttt
    11701 agcttttttc ttctcggcgc gttttgccag tactttcttc gatggcttgc cggtctgctt
    11761 ctcgcttggc gcacgcgttt tcgggcgtaa ctcatcaata acgcgagctt taatcggctc
    11821 ttcaatgtaa cggccaacct tgcccagcag caaatggtca tgggcttcca ccagcgaaat
    11881 tgcggtgcct ttacgaccgg cgcgcgcggt acgtccgata cgatgcaaat aagtatcgcc
    11941 actgcgcggc atatcgaagt taaagacgtg gctgacgtca ggaatgtcga taccgcgcgc
    12001 ggcaacatcg gtggcaacca gtacgtttac gcggccttcg gtcaggcgtt taattgcttc
    12061 gttacgcttg ccttgtacca tctcaccttc gagatagcag ttgttgatgc ccgcttcgcg
    12121 cagccagttt gccagctcat gcacacgttc acgcttacgc acaaacacga ttgagcgagt
    12181 cgcttcaggc tgttttaaca ggtgcaccag caatgcggtt ttatgttcca ggtcatcggc
    12241 gcggtaatac cactgatgaa tttttttgcg ctcacgggtg gacggattgg cagaaacttc
    12301 caccggatct tccagcagac gctcggcaaa gtcctgaatc gcatcgcctt ccagcgttgc
    12361 cgaaaagagc agggtctgtt tacgccagcg agtttcgcca gcaatatgtt cgatatcctg
    12421 agcgaagccc atatccagca tacggtctgc ttcgtcgagg atcagcgttt caaccgcgcg
    12481 gcaatcgaag ttctcttctt ttatgtattg cagcagacgt ccggtcgtgg cgaccacgat
    12541 gtcctgattt tcgctgaaca cttccgcgtg gttcatatag gctacgccgc cggtgatggt
    12601 ggcgatatcc agatgcgtat gtttcgccag ttcgcgggca tgatcggcca cctgcatcgc
    12661 cagctcgcga gttggggtga ggatcaaaat acgcggcgga ccggatttct tacgtgggaa
    12721 atcgagcagg tgctgcaacg ctggcagcag atacgccgcc gttttaccgg tgcctgtcgg
    12781 cgcagaaccg agtacatcac ggccatcgag cgcaggcgga atggcggcag cctgaatggc
    12841 ggtcgggcga gtgaaacctt tatcctggag ggcttccagc aggctttcgt cgagttcaag
    12901 ttcggaaaaa gtcgttacag tcatgttcta cctctgtgtg gggcgctgat tatagacgtt
    12961 acggctgcaa tcttcatctg tttgtatgga tatcgcttct cgggtattgt tctcacccgg
    13021 tgctatcgcc gtttttcctg caaggtttta ttttcatgtc acagtctaca tccgtgcttc
    13081 gtcgtaatgg atttactttt aaacagtttt ttgttgctca cgatcgctgt gcgatgaaag
    13141 tgggaacgga tggcattttg ctgggcgcat gggcaccggt ggctggggtg aaacgttgcc
    13201 ttgatatcgg cgcgggtagc gggttgctgg cactgatgct ggcgcagcga accagtgaca
    13261 gcgtgataat tgatgccgtt gagctggaaa gtgaagctgc cgcgcaggcg caggaaaata
    13321 tcaaccagtc cccgtgggca gagcggatta acgtccatac ggcggatatt cagcagtgga
    13381 tcacacagca gacagtacgc ttcgatttaa tcatcagtaa cccaccttac tatcagcagg
    13441 gagtagagtg cgcgacacct caacgggaac aggctcgcta taccactacg cttgatcacc
    13501 catcgctgct gacctgtgcg gcggagtgca ttaccgaaga gggatttttc tgcgtggtgc
    13561 tgccagagca gattggtaat ggttttactg aactggcatt aagcatgggc tggcatttac
    13621 gtttgcggac ggatgtggcg gaaaacgaag cgcgactgcc gcatcgggtg ctgctggcat
    13681 tctccccgca ggcgggagaa tgctttagcg atcgcttagt cattcgtggg ccagaccaga
    13741 actattccga agcgtatacg gcgctgaccc aggcttttta tctgtttatg taatgattgc
    13801 cgggggaaag gatcgatgga ccggaatgcg tgagcagttc cgggtaatcc agcgtaaaat
    13861 gcagcccccg gctttctttg cgcatcatcg cgcagcgaac aatcaactcg gcaacctgta
    13921 ccagattacg cagctccagc aaattatttg agacgcggaa atgggcgtaa tattcgtcta
    13981 tttcttgttg gagcatggtt atccgccgca gggcgcgttc caggcgcttc gttgtgcgca
    14041 caatgccaac gtaatcccac ataaacagcc gtagctcgtg ccagttatgc tgaattacta
    14101 cccgttcgtc agggttctca acgcggcttt catcccacgg cggtaacgta ctgacgccgt
    14161 gggcataagg catacgtctg gtgatatctt ctgccgccga ccagccatag accagacact
    14221 ccagcaatga attcgaggcc atgcggttag cgccgtgtaa gccggtataa ctcacctcgc
    14281 caatggcata caagccctcg acgtccgtac gcccatgatc atcaaccatt acaccacctc
    14341 aggtatagtg tgcagcaggc acaatcggta ccggttcttg tgtgagatca atccccagcc
    14401 cgagcaattt ttcataaatc atcgggaaat gctggcgaat aaaatcggcg ggcttatggc
    14461 tgatgtcgag gaacatacaa tctgcgccga ggcgtttcat ttcatggtca atggcgcggg
    14521 cgacaatatc gcgcggggcc agttcgccgc gctcatcaaa atcgggcata aaacgcgtgc
    14581 catccgggcg cttgagataa gcgccttcgc cgcgcagtgc ttccgttaac aggaaattgc
    14641 gtgcctgtgg gtgatacagt gcggtagggt ggaactgatt aaattcgaga ttggcaaccc
    14701 gacagcccgc gcgccacgcc atagcaatgc catcgccaga agaaatatcc ggattggtgg
    14761 tgtactgata caccttcgac gcaccgccgg ttgccagtac cactgctttt gcgtggcagg
    14821 tttccaccgt ttctttattt cggttccata cccacgcgcc tacaacccgt cgcgtgcccg
    14881 gcaggccaat tttgtcagag acaatcagat caactgcgtt gctgcgctcc agcacacgaa
    14941 tattcggatg gttctgcgcc ttgctcacca gcgtggattg tacttctcta ccggtggcgt
    15001 cggcggcatg aagaatacga cggtgactat gtccaccttc acgggtcaga tggtagcttt
    15061 cttcgccatt cggttgaacg tgggtatcaa acaaaacccc ctggtcaatt aaccatggca
    15121 cacaggaacg agcattgctg gcgacaaatt caactgcatg gtgatcgcaa ataccagccc
    15181 cggcaatcaa tgtgtcttcc acatgcgagt caatgctgtc agtttcatca aacacggcgg
    15241 caataccgcc ctgggcataa aatgttgaac cttccgttac cgggccttta cttagaacga
    15301 tgacctgatg ctggtcagcc aggcgtagcg ccagtgaaag tccggctgcg ccgctaccaa
    15361 taatcaacac gtcacatgaa tgttcaggga gagtattcat tttctttgtt taatttacta
    15421 aacattgttt ggtcggcata gcatcatgtt gtgcgaataa acacctgcta ttttaatatt
    15481 tgttacagtg gctaaacacg ttgacgcagg gcggcgagaa aaagagaagt tactggctgg
    15541 tggaggatta ggtggtgaaa taaaaaggcc gttgggttac tcttcaggca gttaaatggg
    15601 catttctaca cagataatgc gatgttcaga ttctgtagac ttataatgat agataatgat
    15661 ccgtctacag catgacaaac aaaaacagat gcgttacgga actttacaaa aacgagacac
    15721 tctaaccctt tgcttgctca aattgcagct aatggagtgg cgtttcgata gcgcgtggaa
    15781 atttggtttg gggagacttt acctcggatg agcgagcagt taacggacca ggtcctggtt
    15841 gaacgggtcc agaagggaga tcagaaagcc tttaacttac tggtagtgcg ctatcagcat
    15901 aaagtggcga gtctggtttc ccgctatgtg ccgtcgggtg atgttcccga tgtggtacaa
    15961 gaagctttta ttaaagccta tcgtgcgctg gattcgttcc ggggagatag cgctttttat
    16021 acatggctgt atcggattgc tgtaaataca gcgaaaaatt acctggttgc tcaggggcgt
    16081 cgtccacctt ccagtgatgt ggatgccatt gaagctgaaa acttcgaaag tggcggcgcg
    16141 ttgaaagaaa tttcgaaccc tgagaactta atgttgtcag aagaactgag acagatagtt
    16201 ttccgaacta ttgagtccct cccggaagat ttgcgcatgg caataacctt gcgggagctg
    16261 gatggcctga gctatgaaga gatagccgct atcatggatt gtccggtagg tacggtgcgt
    16321 tcacgtatct tccgagcgag ggaagctatt gataacaaag ttcaaccgct tatcaggcgt
    16381 tgacgatagc gggatactgg ataagggtat taggcatgca gaaagaacaa ctttccgctt
    16441 taatggatgg cgaaacgctg gatagtgagc tgcttaacga actggctcat aacccagaaa
    16501 tgcagaaaac ctgggaaagc tatcacttaa tccgtgattc aatgcggggt gatactcccg
    16561 aggtgctcca tttcgatatc tcttcacgcg tgatggccgc cattgaagaa gagccagtac
    16621 gtcaatcggc gacattgatc ccggaagccc agcctgcgcc gcatcaatgg cagaaaatgc
    16681 cattctggca gaaagtacgt ccgtgggcgg cacagcttac ccaaatgggc gtagccgcat
    16741 gcgtatcgct tgcagttatc gttggcgtcc agcactataa tggacaatct gaaacgtccc
    16801 agcagcccga aacgccggta tttaatacac tgccgatgat gggtaaagcc agcccggtaa
    16861 gcctgggagt accttctgaa gcgaccgcaa acaatggtca acagcagcag gtacaggagc
    16921 agcgtcgtcg cattaatgca atgttgcagg attacgaact gcaacgccga ctccactctg
    16981 aacagcttca gtttgagcag gcgcaaaccc agcaagccgc tgtacaggtg ccaggaattc
    17041 aaactttagg aacgcaatcg cagtaatgaa gcaactttgg tttgccatgt cattagtgac
    17101 aggtagcctg ttattctctg ctaacgcctc ggccactccc gcgtccgggg cgttattaca
    17161 gcagatgaac ctggccagtc agtcactgaa ttacgagctg tcattcatca gcatcaataa
    17221 acagggtgtt gagtctctgc gttatcgaca tgcacgcctc gataaccgtc ctcttgcaca
    17281 attgttgcaa atggatggcc cgcgccggga agtggtacag cgcggcaatg aaatcagcta
    17341 ttttgaaccg ggacttgaac cgttcacgct taatggcgat tacattgttg attctctgcc
    17401 atcgcttatc tataccgatt tcaaacgcct ttctccttac tacgacttta tctccgtcgg
    17461 gcgcacgcgt attgctgatc gtctttgcga agtcattcgc gtggttgccc gagatggtac
    17521 acgctacagc tacatcgtgt ggatggacac cgaatcgaaa ttaccgatgc gggttgatct
    17581 ccttgatcgc gatggtgaaa cgctggaaca atttcgcgtg attgctttta acgtcaatca
    17641 ggatatcagc agcagtatgc agacgctggc gaaggcaaat ttgccgccgt tgctttctgt
    17701 tcctgtaggt gaaaaagcta aattcagctg gacgccaacc tggttgccac agggttttag
    17761 cgaagtttcc agtagtcgac gtccgctacc gacgatggac aacatgccta tcgaatcacg
    17821 tctctattcc gacggattat tcagcttctc ggtaaacgtt aaccgcgcta cgccatcgag
    17881 caccgatcag atgttgcgca ccggacgcag aaccgtcagt acaagcgtac gtgataacgc
    17941 cgaaatcacc attgtcggtg aactgccgcc gcaaacggcg aaacgcattg ccgagaatat
    18001 taagttcggg gcagcgcaat gatcaaagag tgggctaccg tcgtctcctg gcaaaacggg
    18061 caggcgctgg tcagttgtga tgttaaagct tcatgcagca gctgcgcttc acgcgccggt
    18121 tgcggtagcc gcgtgttgaa taaacttggc ccgcaaacca cgcataccat tgtcgtaccc
    18181 tgtgatgagc cgttagtgcc ggggcaaaaa gtggagttgg ggatcgccga aggcagcctg
    18241 cttagctccg cattactggt ttatatgtcg ccgctggtgg gattattcct catcgcttcg
    18301 ctatttcagc tactctttgc ttctgacgtc gcagcattat gtggtgcggt cctcggcggt
    18361 attggcgggt tcctgattgc gcgcggctac tcgcgtaagt ttgctgcccg ggcagaatgg
    18421 caaccgatca tcttaagcgt ggcattgccg cctggccttg tgcgatttga aacatcttcg
    18481 gaagacgcga gccagtgatt tcactggctt tgttgcattt tgcataacaa atgcgaggac
    18541 gtttccagat accacaatcc atacggtatg aacatttcct cgcctcaatg ttgtagtgta
    18601 gaatgcggcg tttctattaa tacagacgtt aagctcagaa cagcgacttc taaaagcctg
    18661 gttaaccggg cattaaggca caataatcat actttatgaa gaatatacgt aacttttcga
    18721 tcatagctca cattgaccac ggtaaatcga cgctgtctga ccgtattatc cagatctgcg
    18781 gtggcctgtc tgaccgtgaa atggaggcgc aggttctcga ttccatggat cttgagcgtg
    18841 agcgtggcat taccatcaaa gcgcaaagcg tgacgctgga ctacaaagcg tctgacggcg
    18901 aaacctatca gcttaacttt atcgacaccc cgggccacgt agacttctcc tatgaagttt
    18961 cccgttcgct ggctgcctgt gaaggtgcat tgctggtggt cgacgccggg cagggcgtag
    19021 aagcgcaaac cctggcaaac tgctacaccg ccatggaaat ggatctcgaa gtggtgccgg
    19081 tactgaacaa gattgacctg ccggcagccg atcctgaacg cgtggcggaa gaaattgaag
    19141 atatcgtcgg catcgacgcc accgatgcag tgcgctgttc agcgaaaacc ggcgttggcg
    19201 tgcaggacgt tctcgaacgt ctggtgcgcg acattccgcc gccggaaggc gatccggaag
    19261 gcccgttgca ggcactaatt atcgactcct ggttcgacaa ctacctgggc gttgtttcac
    19321 ttatccgtat taaaaacggc accctgcgta agggcgacaa agtgaaagtc atgagtaccg
    19381 ggcagaccta taacgccgac cgtctgggca tcttcacgcc gaaacaggtt gaccgcactg
    19441 aactgaaatg tggcgaagta ggctggctcg tatgtgcgat taaagatatc cacggcgctc
    19501 cagtcggcga taccttaacg ctggcgcgta atccggcaga aaaggcgctg cctggcttta
    19561 agaaagtcaa accgcaggta tacgccggtc tgttcccggt aagttccgac gactatgaag
    19621 ccttccgtga cgcgctgggt aaactcagcc tgaacgatgc ctcactgttc tatgagccgg
    19681 aaagctccag cgcgctgggc tttggtttcc gctgcggctt ccttggcctg ctgcacatgg
    19741 agatcatcca ggagcgtctg gaacgtgaat acgatctgga tctgatcacc actgcgccga
    19801 ccgtagtgta tgaagttgaa accacgtcaa gggaagttat ctacgtcgac agcccatcca
    19861 agctgcctgc ggtaaataac atttacgaac tgcgcgagcc gattgcagag tgtcacatgc
    19921 tgctgccgca ggcatatctc ggcaacgtta ttacgctgtg cgtagaaaaa cgcggcgtgc
    19981 agaccaatat ggtttaccac ggtaatcagg tggcgctgac gtacgagatc ccgatggcgg
    20041 aagtggtgct cgacttcttc gatcgcctga aatctacctc gcgtggttat gcgtctctgg
    20101 attacaactt caaacgcttc caggcgtccg acatggtacg tgtagacgta ttaatcaacg
    20161 gtgaacgtgt tgatgcgctg gcgttgatca cccaccgtga taattcgcaa aaccgcggtc
    20221 gcgagttggt ggagaagatg aaagatctga tcccacgcca gcagtttgat atcgccattc
    20281 aggcagcgat tggtacgcac attattgcgc gatccaccgt gaaacagctg cgtaaaaacg
    20341 tactggctaa atgttatggc ggcgatatca gccgtaagaa aaagctgctg cagaagcaga
    20401 aagaaggtaa gaaacgcatg aagcagatcg gtaacgttga gctgccgcag gaagcgttcc
    20461 tcgccattct gcacgtcggc aaagacaaca aataaccctt aggagttggc atggcgaata
    20521 tgtttgccct gattctggtg attgccacac tggtgacggg cattttatgg tgcgtggata
    20581 aattcttttt cgcacctaaa cggcgggaac gtcaggcagc ggcgcaggcg gctgccggtg
    20641 actcactgga taaagcaacg ttgaaaaagg ttgcaccgaa gcctggctgg ctggaaaccg
    20701 gagcttctgt ttttccggtg ctggctatcg tattgattgt acgttcgttt atttatgaac
    20761 cgttccagat cccgtcaggt tcgatgatgc cgactctgtt aatcggtgat tttattctgg
    20821 tagagaagtt tgcttatggc attaaagatc ctatctacca gaaaacgctg atcgaaaccg
    20881 gtcatccgaa acgcggcgat atcgtggtct ttaaatatcc ggaagatcca aagcttgatt
    20941 acatcaagcg cgcggtgggt ttaccgggcg ataaagtcac ttacgatccg gtctcaaaag
    21001 agctgacgat tcaaccggga tgcagttccg gccaggcgtg tgaaaacgcg ctgccggtca
    21061 cctactcaaa cgtggaaccg agcgatttcg ttcagacctt ctcacgccgt aatggtgggg
    21121 aagcgaccag cggattcttt gaagtgccga aaaacgaaac caaagaaaat ggaattcgtc
    21181 tttccgagcg taaagagaca ctgggtgatg tgacgcaccg aattctgaca gtgccgattg
    21241 cgcaggacca ggtggggatg tattaccagc agccagggca acaactggca acctggattg
    21301 ttccgccggg acaatacttc atgatgggcg acaaccgcga caacagcgcg gacagccgtt
    21361 actggggctt tgtgcctgaa gcgaatctgg tcggtcgggc cacggctatc tggatgagct
    21421 tcgataagca agaaggcgaa tggccgactg gtgtgcgctt aagtcgcatt ggcggcatcc
    21481 attaatagcc atcttcgttc acgttgtcgc cgttatggcg acaacgtgaa ttatttatga
    21541 gataaatctc ccgtggctaa cgacatcccc cgtcgttgtg tatagaatat tcccccgaag
    21601 tttaaggttg gcaccttcag gttgccacgg cacacgaaac agcgttggtc cccatatacc
    21661 gctaaactga aactgcagcg aagcagttag cagagccatg tatatcaggt ctgtttcgtg
    21721 tgctgaattg ttgacgcatt tatttattgg tatcgcatga accccatcgt aattaatcgg
    21781 cttcaacgga agctgggcta cacttttaat catcaggaac tgttgcagca ggcattaact
    21841 catcgtagtg ccagcagcaa acataacgag cgtttagaat ttttaggcga ctctattctg
    21901 agctacgtta tcgccaatgc gctttatcac cgtttccctc gtgtggatga aggcgatatg
    21961 agccggatgc gcgccacgct ggtccgtggc aatacgctgg cggaactggc gcgcgaattt
    22021 gagttaggcg agtgcttacg tttagggcca ggtgaactta aaagcggtgg atttcgtcgt
    22081 gagtcaattc tcgccgacac cgtcgaagca ttaattggtg gcgtattcct cgacagtgat
    22141 attcaaaccg tcgagaaatt aatccttaac tggtatcaaa ctcgtctgga cgaaattagc
    22201 ccaggcgata aacaaaaaga tccgaaaacg cgcttgcaag aatatttgca gggtcgccat
    22261 ctgccgctgc cgacttatct ggtagtccag gtacgtggcg aagcgcacga tcaggaattt
    22321 actatccact gccaggtcag cggcctgagt gaaccggtgg ttggcacagg ttcaagccgt
    22381 cgtaaggctg agcaggctgc cgccgaacag gcgttgaaaa aactggagct ggaatgagca
    22441 tcgataaaag ttactgcgga tttattgcca tcgtcggacg tccgaacgtt ggcaaatcca
    22501 cattgttgaa caaactgctg gggcagaaaa tctccatcac ctcccgcaag gcgcagacaa
    22561 ctcgtcaccg cattgtgggg atccatactg aaggcgcgta tcaggcgatc tacgtcgata
    22621 caccgggcct gcatatggaa gaaaaacgcg ccattaaccg cctgatgaac aaagcggcga
    22681 gcagctctat tggcgatgtt gagctggtga tttttgtcgt tgaaggcacc cgctggacgc
    22741 cggacgacga aatggtgctc aacaaactgc gcgaaggcaa agcgccggta atcctcgcgg
    22801 tgaacaaagt ggacaacgtg caggagaaag ccgatctgct gccgcacctg cagttcctgg
    22861 caagccagat gaacttcctc gatatcgtgc caatctctgc cgaaaccggg ctgaatgttg
    22921 acactattgc ggcaatcgtg cgtaagcatc tgcctgaagc gactcatcac ttcccggaag
    22981 attacatcac cgatcgctca cagcgtttta tggcgtctga aatcatccgc gaaaaactga
    23041 tgcgtttcct tggcgctgaa ctgccgtact ccgtgaccgt ggagatcgaa cgtttcgtct
    23101 ctaacgaacg cggcggttgt gacatcaacg gtttgattct cgttgagcgt gaagggcaga
    23161 agaagatggt tattggcaac aaagggtcca agatcaaaac catcgggatt gaagcgcgta
    23221 aagacatgca ggaaatgttc gaagcgcctg tccaccttga actgtgggta aaagtgaaat
    23281 cgggttgggc cgacgacgaa cgcgcactgc gcagtctcgg ttatgttgac gatctttaag
    23341 agtcactccg atggaaggct ggcagcgcgc atttgtcctg catagtcgcc cgtggagcga
    23401 aaccagcctg atgctggacg tcttcacaga ggaatcgggt cgcgtgcgtc tggttgccaa
    23461 aggcgcacgc tctaaacgct ctactctgaa aggtgcatta cagcctttca cccctctctt
    23521 gctacgtttt ggcgggcgtg gcgaagtcaa aacgctgcgc agtgctgaag ctgtctcact
    23581 ggcgctgcca ttaagcggta tcacgcttta cagcggtctg tacatcaacg aacttctctc
    23641 ccgcgtactg gaatacgaga cgcgcttctc tgaactcttt ttcgattact tgcactgcat
    23701 tcagtctctt gcaggtgaca ctggtacgcc agaacccgcg ctgcgtcgct ttgaactggc
    23761 actactcggg catctgggct atggcgtcaa ttttacccat tgcgcgggta gcggcgagcc
    23821 ggtagatggc accatgacgt atcgttatcg cgaagaaaaa gggtttatcg caagcgtcgt
    23881 tatcgacaat aaaacgttca ccggaaggca gttaaaagcg ttaaacgcgc gggaatttcc
    23941 tgacgcagac acactacgcg ccgcgaaacg ctttacccga atggcgctta agccgtatct
    24001 tggcggtaaa ccattaaaga gcagggaact gttccggcag tttatgccta agcgaacggt
    24061 gaaaacacat tatgaatgat gaggattgtc atggctgaat tactgttagg cgtcaacatt
    24121 gaccatattg caacgctgcg taacgcacgc ggtactgctt acccggatcc ggtgcaggcg
    24181 gcgtttattg ccgagcaggc gggagcggac ggcattaccg tgcatttacg tgaagatcgc
    24241 cgccacatta ctgaccgcga tgtgcgcatc ctgcggcaga cgctggatac ccgaatgaat
    24301 ctggagatgg cagtgaccga agagatgctg gcgatcgccg ttgagacgaa gccacatttt
    24361 tgctgcttgg taccggaaaa gcgtcaggaa gtaacaaccg aaggcggcct ggacgttgca
    24421 gggcagcgtg aaaaaatacg cgatgcctgc aaacgtctgg cagatgccgg gattcaggtt
    24481 tctctgttta tcgacgccga tgaagagcag atcaaagctg cggcagaggt tggcgcaccg
    24541 tttatcgaga tccacaccgg ttgctatgct gatgccaaaa ctgacgccga acaggcgcaa
    24601 gagctggtgc gtatcgccaa agccgcgacc tttgccgcta gtctcggtct gaaagttaac
    24661 gccggacacg gtctgaccta tcacaacgtg aaagccattg ccgccatccc tgagatgcat
    24721 gaactgaata tcggtcatgc cattattggc cgtgcagtga tgaccggact gaaagatgcg
    24781 gtggcagaaa tgaagcgtct gatgctggaa gcgcgtggct aatggcaata ttaggtttag
    24841 gcacggatat cgtagagatc gctcgcatag aagcagtgat cgcccgatcc ggtgagcgcc
    24901 tggcacgccg cgtattaagc gataacgaat gggcaatctg gaaaacgcac caccagccgg
    24961 tgcgttttct ggcgaagcgt tttgctgtga aagaagccgc agcaaaagcg tttggcaccg
    25021 ggatccgcaa tggtctggcg tttaatcaat ttgaagtatt caatgatgag ctcggcaaac
    25081 cacggctacg gctatggggt gaggcattaa aactggcgga aaagctcggc gttgtaaata
    25141 tgcatgtaac gctggcagat gaacgccact atgcttgtgc cacggtaatt attgaaagtt
    25201 aaaataatac cgttctcttt aaaaagtaaa acccccgaac tggtaaggaa cgggggtttt
    25261 actttttaac tttaattttg tgcagcatgc accctcaaca acagcgatta tggatcagtg
    25321 tacttatctt ttacaagcaa cttgcaatct ttagcataaa aactcgagcc tttacgaaga
    25381 aagcaatatt gatggaaaga ttaacgtgac cgccaattcg taagtacatt aaaattggct
    25441 tcgttattga agattttgct gtgctttaca ccatgccaca gaattccccc attgaaacga
    25501 gtggtgtcgt caaagctctg gtgtggagtg cagcatgcac cctcaataac tcgcacgttc
    25561 agttttgggg agatgtaagg gctaatctga atggctgcat tccttgttta aggaaaaacg
    25621 aatgactgat tgccgatacc tgattaaacg ggtcatcaaa atcatcattg ctgttttaca
    25681 gctgatcctt ctgttcttat aacacaagga aacgtactta aggtgcgtcc ggtgaaccaa
    25741 tcggacgcgc ctttaataac tataaataag tgtcttggca gatactatat aaattaactt
    25801 agtgaatgat tatgctaatg tcatcaatta aataaatata atggcgttaa ggcttcccag
    25861 taatataatt aatactctac ttccagagta gaatattaaa ttttatccgc gtggtgcatc
    25921 agcacaaatt tatcccacaa ctgttcttct gtctcgacat gcgccggatc tttcacaata
    25981 gtattgggga tcgggcacac cttctggcag gttggtgtct cgtagtgccc tacgcattcg
    26041 gtacacttat cgctgttaat ttcgtagata tgatctccca ttgaaatcgc ctcattcggg
    26101 cattcgggtt cacacatatc acaattaata cagcgtttag taattagtaa agacatttca
    26161 atggattacc gttaaatcat tttaaaatca gtaagttgta tcgagtttgt atgctttact
    26221 gtcattaact tactgtatgt tgatccagtg tatttaacct tgataaactc agtccagcaa
    26281 cacaaaaccg caacacattg cattttgtcc cgtagaaaag acttgtatgt gtgagcttgt
    26341 tttctgcgcc tacgcagata aggattgaga atgccgcgca ctgtaacaca taatccggat
    26401 agccccaata atgacgatgt tttagccgca tctgaaaaat gggacgcctg taaacccccc
    26461 tataccagcg cacacatgaa aatctgtgtt gctgccgcca aaatcatcct cgctgcttcc
    26521 ggcgtggctc gctgttccaa atacgaaaaa gagaactatc tccgtatcga tttcagcaaa
    26581 gccggtaagg ttacatttta cgccgagttt ccaaaaaaga tgggcctcaa aggtaaaaag
    26641 ctcggcgagt ggccggagct cgttatccag ctggcgcgcg aaaaagcact aggtatggct
    26701 gacggtggac tgcgggcaga gtccgtacat gcagcgctgg aaatgtaccg ggatgaccca
    26761 aagccaaagt agcccggcag aagctgagcc cggacagttt cacaacctac ggggtgcgta
    26821 tcgaccggat taaagtaacg ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc
    26881 ggctggtgga agtgctggac gagtggatcg ccactcgctc gaacaataac gccctggagt
    26941 tgtttgccga gctccgtcgg ttctggaagt tctgcgcacc tactctttgc aacggccgca
    27001 atgttgccgc cagtctgcca gatgattatg tttcctcccg cgtacagaaa cctaccccca
    27061 cacggctttt taccgatatt gaatcaatcg cccgactctg gctcaatgtt gctgcctgca
    27121 cctctgtaca ccagaagaat gctgttcgct tcatgatcat cactggtgtt cgtccgatta
    27181 atgtccataa cctgcgctgg gactacgttt acgaggaggc tggtgaaatt gtttatccgg
    27241 aaggggttat cggcatgcga ggggctatga aaacacaaaa ggctttccgc ctgccgataa
    27301 cgcctgagat ccggcggatt atcgacgagc agaaagcctg gcgtgattca gttcctgagt
    27361 gcaacaggga ttatgtattt ttgcagccac gtgatccaat gcagccattt tcaaaacgat
    27421 cactggataa gctggtgaaa acatacagcc cggacggggc tgtaaaatga ggtgtactgg
    27481 caatagcgga cactaccatt tgttcttttt ttaagcagcc atctgatgat
//
LOCUS       DAJTSF010000052        26789 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000052 DAJTSF010000000
VERSION     DAJTSF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.052"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..227)
                     /locus_tag="OO335_002297"
     CDS             complement(<1..227)
                     /locus_tag="OO335_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002664.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNG"
     gene            complement(343..894)
                     /gene="maa"
                     /locus_tag="OO335_002298"
                     /pseudo
     CDS             complement(343..894)
                     /gene="maa"
                     /locus_tag="OO335_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(1066..1284)
                     /gene="hha"
                     /locus_tag="OO335_002299"
     CDS             complement(1066..1284)
                     /gene="hha"
                     /locus_tag="OO335_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6002665.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(1310..1684)
                     /gene="tomB"
                     /locus_tag="OO335_002300"
     CDS             complement(1310..1684)
                     /gene="tomB"
                     /locus_tag="OO335_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6002666.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(2230..5379)
                     /locus_tag="OO335_002301"
     CDS             complement(2230..5379)
                     /locus_tag="OO335_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6002667.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(5402..6595)
                     /gene="acrA"
                     /locus_tag="OO335_002302"
     CDS             complement(5402..6595)
                     /gene="acrA"
                     /locus_tag="OO335_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6002668.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            6737..7384
                     /gene="acrR"
                     /locus_tag="OO335_002303"
     CDS             6737..7384
                     /gene="acrR"
                     /locus_tag="OO335_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6002669.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            7512..10874
                     /gene="mscK"
                     /locus_tag="OO335_002304"
     CDS             7512..10874
                     /gene="mscK"
                     /locus_tag="OO335_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6002670.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(11086..11247)
                     /gene="rsmS"
                     /locus_tag="OO335_002305"
     CDS             complement(11086..11247)
                     /gene="rsmS"
                     /locus_tag="OO335_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6002671.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(11261..11788)
                     /gene="priC"
                     /locus_tag="OO335_002306"
     CDS             complement(11261..11788)
                     /gene="priC"
                     /locus_tag="OO335_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6002672.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            11858..12235
                     /locus_tag="OO335_002307"
     CDS             11858..12235
                     /locus_tag="OO335_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6002673.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12388..12939
                     /gene="apt"
                     /locus_tag="OO335_002308"
     CDS             12388..12939
                     /gene="apt"
                     /locus_tag="OO335_002308"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6002674.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            13068..14993
                     /gene="dnaX"
                     /locus_tag="OO335_002309"
     CDS             13068..14993
                     /gene="dnaX"
                     /locus_tag="OO335_002309"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6002675.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    14326..14390
                     /gene="dnaX"
                     /locus_tag="OO335_002309"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            15046..15375
                     /locus_tag="OO335_002310"
     CDS             15046..15375
                     /locus_tag="OO335_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6002676.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            15375..15980
                     /gene="recR"
                     /locus_tag="OO335_002311"
     CDS             15375..15980
                     /gene="recR"
                     /locus_tag="OO335_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6002677.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            16090..17964
                     /gene="htpG"
                     /locus_tag="OO335_002312"
     CDS             16090..17964
                     /gene="htpG"
                     /locus_tag="OO335_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6002678.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            18145..18789
                     /gene="adk"
                     /locus_tag="OO335_002313"
     CDS             18145..18789
                     /gene="adk"
                     /locus_tag="OO335_002313"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6002679.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            18921..19883
                     /gene="hemH"
                     /locus_tag="OO335_002314"
     CDS             18921..19883
                     /gene="hemH"
                     /locus_tag="OO335_002314"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6002680.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(19880..20839)
                     /gene="aes"
                     /locus_tag="OO335_002315"
                     /pseudo
     CDS             complement(19880..20839)
                     /gene="aes"
                     /locus_tag="OO335_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            21014..22324
                     /gene="gsk"
                     /locus_tag="OO335_002316"
     CDS             21014..22324
                     /gene="gsk"
                     /locus_tag="OO335_002316"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017799384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6002681.1"
                     /translation="MQTLLPCKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQT
                     LVDIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMH
                     NYSVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGE
                     SGERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYA
                     KKYNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKA
                     LDWVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQN
                     PLRVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLT
                     YSSLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(22457..24133)
                     /locus_tag="OO335_002317"
     CDS             complement(22457..24133)
                     /locus_tag="OO335_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6002682.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(24371..25591)
                     /gene="fsr"
                     /locus_tag="OO335_002318"
     CDS             complement(24371..25591)
                     /gene="fsr"
                     /locus_tag="OO335_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6002683.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            25809..>26789
                     /gene="ushA"
                     /locus_tag="OO335_002319"
     CDS             25809..>26789
                     /gene="ushA"
                     /locus_tag="OO335_002319"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6002684.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWED"
BASE COUNT         6730 a         6921 c         6914 g         6224 t
ORIGIN      
        1 aaaccattaa gcaggataaa taatacaacc agtagccaga cagagcctga cagcgtttcg
       61 gactgcaaca tcaccgccag ggtggcaaaa aaagcaacgt acataccgat aaacagcccc
      121 ggatgtttac ggataaaact gatactaaag cggggtttgt tgtcgcgttt ttcgcgggta
      181 ttcagagact caatcgtttc ggtcagcagg cgttgtattt cggtcatcgt ttgccttcgt
      241 ggtatctgca actttccagg gaacacccta ttttaacgtg ggaatagtag aggggaagta
      301 acagatctac cacaattttg cataacagtt gcgaaaaacc gattacaatt ttttaattat
      361 tctggctgga ttaccgccca cgacaacgtt gtccgggaca ccttttgtga caactgcacc
      421 tgaagctacc acgacgttat caccaatggt cacaccaggg ttaatgaccg cgcgtccgcc
      481 aatccagacg ttattaccga tggtgacggg tttccccagt tcagcaccgc tattacgtgc
      541 tacagggtcg atgggatgtg ttgccgtgta gatatgaacg cctggtgcca acatacagtt
      601 atcaccgatg cgaatagggc agacatcaag catcacgcaa tcgaagttgg cgaaaaaatt
      661 attgccgaga aaaatgttat agccataatc acagcgaaac gttggctcaa tataagcctc
      721 tgtcacctga ccgaataaat tagcgagaat ttactggcgt aatgtgtgct cttccgccag
      781 ggaatgattg tatcggtgaa taagctgacg agcgcgcagg cgatcgcgag ataacgtctc
      841 atctgccgag cgatacaact caccagcaat catcttttct ttttctgtgc tcattactga
      901 cctccggttg ctgaaaatgc ggcaacacag taagacgaaa agtcagtgaa gagaacgttc
      961 tcattacatc tgtgtgataa agatcacata gggtatgtct tcatggcgaa aaagtataaa
     1021 attcttaata aacagccggt tatagcaccg aaagcgaatt atttattagc gaataaattt
     1081 ccatactgag gaagggatct tgtcgtacag tttattcatg gtcaattcgg cgaggcggtg
     1141 atctgcggct gagtaaaata ccgccagttc attatctgat aattcgtatt tatttttctc
     1201 gataacacgc tccagcgtgt caattgtctg gcaacgacgt aaacgcatta aataatcggt
     1261 ttttgttaaa ggtttttcgg acatacttct acccatggtt gtaataattc taacaagata
     1321 aactcgcagg attctctttc gtcgcgttga caaaacaacg gaatagtcga ttacctgact
     1381 tccgccattt ctgaagagcc tgcatattaa taccataact gctgaacaac ataaaggtgt
     1441 catccagata ttcgtcgatc tgctcaatga gcttattgtc ttcattatac ttaattttgt
     1501 aattaagtgc gaaggtcgca atatgctcaa tcagttcatt caactggagg ttgatcgccg
     1561 aggttgggtc gttaacccaa ccatgattgc tttcttcaag gtttgcaaga cagtcatgat
     1621 acagggtttc acagagaaac ttaagctgtg cgatatcatg tcttttgggt gagtattcat
     1681 ccataacgcg tccccttctt agtggttgaa ctaacggaca cctttcggga tggaaaaaac
     1741 ttactgacct agacttgccc tctcgtttgt tagcttaact ataagccact ctttgcaggt
     1801 tttcatcgca ttattacgaa acattacaat taggaaggaa tcgggacagg actcactttt
     1861 tcatactagc actaacccgc agcaggtaaa agcagtttgc tcgaactgtt cataacattt
     1921 caggattatc gccagatgaa attggtgaca ctaataccag gattgctctg aatatgacgt
     1981 aataaccgag gaatgaataa agaattaccg caaataatta agaatagcct ctaaatgatt
     2041 atggattatt aaatctatta gcgctactta atataaatta actaacaatc agtatttttt
     2101 atgaatttta tacgtggtta atactggttt tcgtatgaga tcctgagttg gtggttcaat
     2161 tactccttaa tgttcgtagg ttatgcataa aaaaggccgc ttacgcggcc ttagtgatta
     2221 catgttgtat caatgatgat cgacagtatg gctgtgctcg atatcttcat tcttgcggct
     2281 aaagcggcgg cgaaccacca caaagaatac cggaacgaag aagattgcca gtaccgttgc
     2341 ggtcaccatc ccgcccatta caccggtacc tactgcgttc tgcgcgccgg aaccagcacc
     2401 agtactgata accagcggca taacgccgag gataaacgcc agcgaggtca tcaggatcgg
     2461 acgtaaacgc atccgcaccg catcaagcgt cgcttcaatc agacctttac cttctttatc
     2521 catcaagtct ttggcgaatt cgacgataag tatcgcgttc ttcgccgaca acccaatggt
     2581 tgtgagcagt cctacttgga agtaaacgtc attggtcagg ccacggaagg tggcagccag
     2641 caacgcaccg ataaccccca gcggaacgac cagcataacg gagaacggag tcgaccagct
     2701 ctcgtacagc gccgccagac acaggaatac gacaatcaac gaaatcgcgt acagtgaagg
     2761 tgcctggttg ccggagagac gttcctgata ggacatcccc gtccagtcat agccaacacc
     2821 ggtaggcagt ttgctcgcca gttgttccat cagctccatt gcttcaccgg tacttttacc
     2881 cggtgccgcc tggcctaaga tttccatgga tggcaggccg ttgtaacgtt ccagacgcgg
     2941 cgaaccgtac tcccaacgag aagaggagaa cgccgagaat ggcaccatct gaccatcagc
     3001 agcacgaaca taccagtcgc cgatatcatc cggcagcata cggtatttcg cttctgacat
     3061 gacgtaaact ttcttcacac gaccgcggtc gataaagtcg ttcacatagc taccgcccca
     3121 tgcagcgccc agagtggtgt taatgtcgtt gatagaaaca cccagcgcct gcgctttttc
     3181 ctggtcgata tcaatcttaa actgcggggt atcttccaga ccgtttggac gtacgctggt
     3241 cagcatatca gggtgcttcg ctgcttctgc aagcagctgg ttacgcgcct gagtcagttt
     3301 ttcgtgacca aggccagcct ggtcaatcag ctcaaagtca aagccggttg cagtacccag
     3361 ttccacgatt gcgggcaggt taaaggcgaa aaccatcgca tctttgattt gcgagaaagc
     3421 gcgtgttgca cgcatggtaa tcgcttcaac tttgttttct tcgcccggac gatcggccca
     3481 gtccttcaag gaaacgaacg caataccggt attctgacca cgtcccgcaa agccgaagcc
     3541 gttaacggcg aacaccgact caacgttgtt cttttctttg gtcagatagt aatgcgttac
     3601 ctcattgagc actttctgcg tacgttcctg cgttgcacct gctggcagct gaaccatggt
     3661 cataaatacg ccctggtcct catctggcaa gaaggagctt ggcagacgca cgaacagata
     3721 ggccatgccg accacgatga tcagatacag cactaggtaa cgccccgtac tgcgcagaat
     3781 accgcctacg ctgtcggtgt agtggtgcgt gctcttctcg aacatgcggt tcaaccagcc
     3841 gaagaagcct tttttacctt ccccgtgatc gcctttggca atcggtttca gcatggtggc
     3901 acaaagagcc ggagtcagga tcaacgctac cagtaccgac agcgccattg ctgaaacaat
     3961 ggtaatagag aactgacgat agatagcacc agtagaaccg ccaaagaagg ccatcggtac
     4021 gaataccgcc gacagtacca tcgcgatacc gaccagagcg ccctgaatct gccccatcga
     4081 cttacgggta gcttcttttg gcggcaaacc ttcttccgcc ataacacgct ccacgttttc
     4141 taccacaacg atagcgtcat ccaccaacaa gccgatggcg agcaccatcc cgaacattgt
     4201 tagcgtgttt atcgagaagc caaaggcggc aaggacggca aaggtcccga gcaataccac
     4261 cggtacggca atggtcggaa tcaacgtcgc gcggaagttc tgcaggaaca gatacataac
     4321 caggaacacg aggatgatcg cttcgaccag cgttttaacc acttcgtgaa tagagatttt
     4381 cacgaacggc gtggtgtcgt acgggtaaac aattttcaga cccgacggga agaacggttc
     4441 catcttcgcc agttcagcac ggattgccgc agcggtatcc agcgcgtttg caccggtcgc
     4501 cagcttgatc cccagaccgg aagcaggttg gccgttaaac tctgcgatga tgtcgtagtt
     4561 ctcaccaccc agctcaatct tcgccacatc acgcagcagt acgcgggaac catcctgatt
     4621 cactttcagc aggattttgc cgaactcttc agtagaggtc agacgcgtct gagcaataat
     4681 agaggcgtta agctgttggc ctttcaccgg cggcgtacca ccgagctgac ccgccgcaac
     4741 ctgggcgttc tgcgctttga tggcggtaat gacatcaacc ggcgttagct ggaatttgtt
     4801 cagctcattc gggttcatcc agatacgcat cgcgtactgt gaaccgaaca actgaacatc
     4861 acccacgccc gacgtacggc tgatggcatc tttcatattc gccgctacgt agtcagagat
     4921 atcctcctgc gtcatggtgc catcggtgtt gataacgccg acaaccatca ggaagctgct
     4981 ggatgatttc tcaacgctca ccccttgctg ctgaacttct tgcggcagca acggcatcgc
     5041 cagctgcagt ttgttctgca cctgaacctg cgcgatatcc gcatcagtac cagactcaaa
     5101 ggtcagggtg atctgcacgg tacccgtgga gtcactgtta gaggacatgt acatcaggtt
     5161 atcgataccg ttcatattct gttcgataac ctgtgtcacc gtgtcctgca ctgttttcgc
     5221 atcagcgccg gggtaggagg cggagatcgt tactgccggc ggtgcaatcg taggatattg
     5281 cgccaccggc agtttgagga tcgccagccc ccctgccaac atgatgataa tggcgatcac
     5341 ccacgcaaaa atcgggcgat cgataaagaa attaggcatg tcttaacggc tcctgtttaa
     5401 gttaagactt ggactgttca ggctgagcac cgcttgcggc ttgctggtta ttatcagcgg
     5461 taacttcttg tgcttttacc tggacaccag gacgcacttt ctgcagccca cttattacta
     5521 cgcgatcgcc tgctttcaga ccttctgtca ccagccactt atcgccaata gcctggcttg
     5581 caacgatcgg acgggtttcc actttgtcat ccgcgccaac taccagtacg gtggcatcac
     5641 cacgcggcgt acgggttacg ccctgttgcg ggactaaaat agcgtttgga ttaagccctt
     5701 cttccagacg tgcgcgcacg aacatacccg gcagcagagt gtgatccggg ttcgggaaga
     5761 tagcgcgtag ggtgatagac ccagtggtct gatcaacggt aacgtcagag aattccagcg
     5821 taccgtcctg cgggaactta atgccgtcac tggtgatcag cgacactttg gctttgccgt
     5881 tctcttgttt cagcgtgcca ttcgccagtt cctgtttcag gcgaaggaag tcgttgctgg
     5941 actgggtcac atcaacgtag atcggatcaa gttgctgcac ggttgccagc acagtcgcct
     6001 gaccgttctg taccaatgcg ccttccgtca cgttcgactt accaatgcga ccgctaatcg
     6061 gagaggtgac tttggtgtaa gccagattga tccgcgcagt ttcaacggca gctttcgccg
     6121 cagttaccgc agcattcgcc tgttgcgcat cagccagagc ctgatcgtac ccttgcttac
     6181 tgatgtactg agtaccgagc agtttctgat aacgattcac cgtcaattgc gcgatattgg
     6241 ctgcagcctg ggctttcgcc agatcacctt tcgcactgtc gtatgtcgcc tgataggtcg
     6301 caggatcaat ctgatagaga gagacaccag cttcgatgtc gctaccttct ttgaaattac
     6361 gcttcaggat aatcccgcta acttgaggac gaacttctgc gatccggtag gcactggtgc
     6421 gacctggaag ctcggttgtg atctgcagag gttcagtttt gactgttact acgccaacgg
     6481 cgggcatctg ctggccacct tgttgggcct gtttgtcgtc acatcctgtt agggctaagc
     6541 tgcctgagag catcagaacg accgccagag gcgtaaaccc tctgtttttg ttcatatgta
     6601 aacctcgagt gtccgatttc aaattggtca atggtcaaaa gttaataaac ccattgctgc
     6661 gtttatatta tcgtcgtgct atggtacata cattcacaaa tgtatgtaaa tctaacgcct
     6721 gtaaattcac gaacatatgg cacgaaaaac caaacaagaa gcgcaagaaa cgcgccaaca
     6781 catcctcgat gtggctctac gtcttttctc acagcagggg gtatcatcca cctcgctggg
     6841 cgagattgca aaagcagctg gcgttacgcg cggtgcaatc tactggcatt ttaaagacaa
     6901 gtcggatttg ttcagtgaga tctgggaact gtcagaatcc aatattggtg aactagagct
     6961 tgagtatcag gcaaaattcc ctggcgatcc actctcagta ttaagagaga tattaattca
     7021 tgttcttgaa tccacggtga cagaagaacg acgtcgatta ttgatggaga ttatattcca
     7081 caaatgcgaa tttgtcggag aaatggctgt tgtgcaacag gcacaacgta atctctgtct
     7141 ggaaagttat gaccgtatag aacaaacgtt aaaacattgt attgaagcga aaatgttgcc
     7201 tgcggattta atgacgcgtc gggcagcaat tattatgcgc ggctatattt ccggcctgat
     7261 ggaaaactgg ctctttgccc cgcaatcttt tgatcttaaa aaagaagccc gcgattacgt
     7321 cgccatctta ctggagatgt atctcctgtg ccccacgctt cgtaatcctg ccactaacga
     7381 ataaccctga atctgactcc aggatttttc ctggacattt tcgtcgttgc tattctggtt
     7441 cactgcgtcg tgatattctt gcggtttgac tttttcaggt cgttcttcag gttcagaaac
     7501 cttcattcat catgactatg ttccagtatt acaaacgatc acggcatttt gttttttcag
     7561 catttattgc ttttgttttt gtcttgttat gccagaacac ggcgtttgcg cgggcgtcat
     7621 cgaatggtga tctgccgaca aaagcggacc tgcaggcgca acttgactca ctaaataaac
     7681 aaaaagatct ttctgctcag gacaaactgg tgcagcagga tctgacagat acattagcca
     7741 ccctcgataa aatcgatcgc ataaaagaag agacagttca gctacggcaa aaagtcgctg
     7801 aagcgccgga aaaaatgcgc caggcgaccg cggcgttaac agcacttagc gatgtcgata
     7861 acgacgaaga aacgcgcaaa attctgagta cgctttcgtt gcgccagctg gaaactcgcg
     7921 ttgcccaggc gctggacgat ttgcaaaacg cacaaaacga tctggcgtct tataacagcc
     7981 agctggtttc gttacagacg cagcccgaac gcgtgcaaaa tgcgatgtat aacgcttcgc
     8041 agcagctgca acaaattcgc agtcgtctgg atgggactga tgtcggcgag actgccttac
     8101 gtcccagcca gaaagtgtta atgcaggctc agcaggcgtt gctgaatgcg gagattgacc
     8161 agcagcgtaa aagcctggaa gggaacaccg tcttgcagga taccttgcaa aagcaacgtg
     8221 attacgtgac ggcgaacagc gcccgtctgg agcaccagtt gcagctgttg caagaagcgg
     8281 taaacagcaa gcgcctgact ttaactgaaa aaacggcgca ggaagccgtc tccccggatg
     8341 aagccgcgcg tattcaggct aatccgctgg tgaaacagga actggaaatt aaccagcagt
     8401 taagtcagcg tctgattacc gcgactgaaa acggtaatca gttgatgcag caaaacatta
     8461 aagtcaaaaa ctggctggag cgggcgctgc aatcggaacg caatattaaa gagcagattg
     8521 ccgtcctgaa gggcagcctg ctgttgtctc gtatccttta ccagcaacaa caaacgctgc
     8581 cctcggcgga tgaactggaa aacatgacca accgcatcgc ggatttgcgt ctcgaacagt
     8641 ttgaagttaa ccagcagcgt gatgcactct tccagagcga tgcgttcgtc aacaaactgg
     8701 aagaaggtca caccaacgaa gtcaacagcg aagttcacga tgcgttattg caagtggttg
     8761 atatgcgtcg cgaattgctg gatcaactca acaaacagtt gggtaaccag ctgatgatgg
     8821 ccattaacct gcaaatcaac cagcagcagt taatgagcgt atcgaaaaac ctgaaatcca
     8881 tcctgactca gcaaatcttt tgggtgaaca gtaaccgtcc aatggactgg gactggatca
     8941 aagcgttccc gcaaagcctg aaagatgaat ttaagtcgat gaaaatcacg gtgaactggg
     9001 aaaaagcctg gcctgccgtt tttatcgctt tcctcgccgg tttgccgctg ctgttgattg
     9061 ccgggctgat ccactggcgt ctgggctggc tgaaagcgta tcaacagaaa ctggcttccg
     9121 ctgtgggttc cctgcgtaac gatagccagc tcaacacacc aaaagcgatc cttatcgacc
     9181 tgatccgcgc gctgccggtg tgcctgatta ttctcgcggt tggcctgatt ctgttgacca
     9241 tgcagctcaa catcagcgaa ctgctatggt cgttcagtaa aaaactggcg attttctggc
     9301 tggtgtttgg cctgtgctgg aaggtactgg agaaaaatgg cgttgccgtg cgtcacttcg
     9361 gcatgccgga gcagcagacc agccactggc gtcggcaaat tgtccgcatc agtctcgcgt
     9421 tgctgcctat ccatttctgg tctgtggtgg cagaactttc cccgctgcat ctgatggatg
     9481 atgtgctggg gcaggcgatg attttcttca acctgctgct gattgccttc ctggtatggc
     9541 caatgtgccg cgaaagctgg cgtgataaag agtcgcacac catgcgacta gtcaccatta
     9601 ccgtgctgtc gattatcccg attgcattga tggtgctgac tgccacgggt tatttctaca
     9661 ccacgctgcg cctctccggg cgttggattg aaaccgttta tctggtgatc atctggaact
     9721 tgctctacca gacggtattg cgcggcttaa gcgtagcggc gcggcgtatc gcctggcgtc
     9781 gtgcgctggc gcgtcggcag aatctggtga aagagggcgc agaaggtgcc gaaccgccgg
     9841 aagaacccac cattgcactg gagcaagtta accagcagac gctgcgtatt accatgttgc
     9901 tgatgtttgc gctgttcggt gtcatgttct gggcaatttg gtccgatttg atcaccgtgt
     9961 tcagctatct cgacagcatc acgctctggc attacaacgg cactgaagct ggcgctgcgg
    10021 tggtgaaaaa cgtcaccatg ggcagtctgt tgtttgcgat tatcgcctca atggtggcct
    10081 gggcgttgat tcgcaacctg cctggtttac tggaagtgct ggtgctctcg cgactgaata
    10141 tgcgccaggg cgcgtcgtat gccattacta ccatccttaa ctacatcatt attgccgttg
    10201 gtgcgatgac ggtgttcgga tcgctgggcg tctcttggga taaactccag tggctggccg
    10261 cagcattatc cgtaggtctt ggttttggtt tacaagaaat tttcggtaac ttcgtctccg
    10321 gtttgatcat tctgttcgaa cgtccggtgc gtattggcga tacggtaacc attggtagct
    10381 tctcggggac ggtaagtaag atccgtattc gtgcgacaac gattaccgat ttcgatcgca
    10441 aagaagtgat catcccgaac aaagcgtttg ttaccgagcg tctgatcaac tggtcgttga
    10501 ctgacactac tacgcgtctg gtgatccgtc tcggcgtggc ctatggctcc gatctggaaa
    10561 aagtgcgtaa agtgttactg aaggcggcga ctgagcaccc aagggtgatg cacgaaccaa
    10621 tgccggaagt cttctttacg gcatttggtg ccagcacgtt ggatcatgag ctgcgtctgt
    10681 atgtgcgtga actgcgtgac cgtagccgta ctgtcgatga gctgaaccgt actatcgatc
    10741 agctgtgccg tgaaaacgac atcaacattg cctttaacca gcttgaagtg catctgcaca
    10801 acgagaaggg cgatgaggtg acggaagtaa aacgcgacta caaaggcgac gatccgacac
    10861 cagcggttgg gtaataacca aagggggcga catttagttg ccccgagatt actgacaaag
    10921 tgcgcgttgt tcatgccgga tgcggcgtga ccgccttatc cggcctacga aaccgcaaga
    10981 attcaatata ttgcaggagc ggtgtaggcc tgataagcgt agcgcatcag gcagttttgc
    11041 gtttgcccgc aatcttaggg gacatttagc ggccccattt atttctcact tttccgcctc
    11101 atcatcgtgc gttaatttct tttcataatc acgctttaca atatccagcg cgcgcagcac
    11161 ggtactggca gggatctgat tttcctccag cagcacaatc aaatcgacag ccagtttgac
    11221 atcgtcaggg gcattttcaa gtgacatact ctttccattg ctagcgggtt aagcgcgcta
    11281 gcctgttttc gattttttcc agcgcatggc ggcagcgtgc caggcgcgct tcataggctt
    11341 ccacttcacg atgcagcgtt tgctgttcca cgagatcggt cacccgcgcc aggcgggctc
    11401 tgcgttcggc aaccatctca cgtagccgcc gctcaaaatc ctgatgctga atacgtttac
    11461 gctgccagcg ggaaattttc ggtggcgcac tgtcccactc gcgcaatgac caggcggagg
    11521 cttcacgcgc gatggcttcc agttgtgccg ccagatgttc cgccagccag gccacttgcg
    11581 gcagctgttg ctgctcaact gcatgacgaa gcgcagccag attatcgccc gcctcgtcga
    11641 gacaagactg tagtgttgtc gcacgagtct gaaaaagatg cctgtcgaaa cgagcactta
    11701 gcgtggcgaa ctgtgacacc ggggcacaac gctgacgcag cgtagcgagc tgtccttcca
    11761 gtttttccag cagcagggcg gttttcacga taagacctca atgaaaatga taattgttat
    11821 gctaaagtag ccactatgta agctgacaac actcaatatg caacgaatca ttttaatcat
    11881 cattggctgg ctggcggtag tgctgggtac gctgggcgtg gtattaccgg tattaccgac
    11941 gacgccgttt atcctgctgg cggcctggtg ctttgcccgt tcttccccgc gctttcacgc
    12001 ctggttgctg taccgctcat ggtttggcag ctatctacgt ttctggcaga aacatcatgc
    12061 gatgccgcgc ggcgtcaaac cgcgggcgat tttgcttatt ttgctcacgt ttgccatttc
    12121 tctgtggttc gtccagatgc catgggtgcg catcatgttg ctggtaattc tcgcctgttt
    12181 gcttttctat atgtggcgaa ttccggtgat tgatgaaaag caagaaaagc actgaagcac
    12241 aacaatcgca gttgcaatta ttgcgtacag ccagtacatt ctggcgtttt cgagcacagg
    12301 cgcaggcggt caaaggttaa acaacagtta cttttgatac gtttaaaacg cgccgtgagt
    12361 accaccgtaa caagcaggca tacacttatg accgcgactg cacagcagct tgagtatctc
    12421 aaaaatagca tcaaaagcat tcaggactac ccaaaacccg gcattctttt ccgcgatgtc
    12481 accagcttac tggaagaccc gaaagcttac gctctcagca tcgacttgct ggttgagcgt
    12541 tacaaaaatg cgggcattac caaagttgtc ggcaccgaag cgcgtggctt cttgtttggc
    12601 gctccggtag ctctgggtct gggcgttggc tttgtaccgg tccgtaaacc gggcaaactg
    12661 ccgcgtgaaa ccatcagtga aaattacgac ctggaatacg gcaccgatca gctggaaatc
    12721 cacgttgatg ccatcaaacc gggcgacaaa gttctggtgg tggacgatct gctggcaacc
    12781 ggcggcacta tcgaagcgac cgttaaactg atccgtcgtc tgggtggtga agtggctgac
    12841 gctgcgttca ttatcaacct gtttgatctc ggcggcgaac agcgtctcga aaaacagggc
    12901 attaccagct acagccttgt cccgttcccg ggccattaat tatcgccagt cttgtgctgc
    12961 ccacgctacg gacagcacaa gatgtgcatt cagcctcgcc gttctgacgg ggctgtgtta
    13021 gcattacccc ttcgtgaatc caccttccag cgtttcagag cctgccaatg agttatcagg
    13081 ttttagcccg aaaatggcgc ccacaaacct ttgctgacgt cgtcggccag gaacatgtgc
    13141 tgaccgcact ggcgaacggc ttgtcgttag ggcgtattca tcatgcttat cttttttccg
    13201 gcacccgtgg cgtcggaaaa acctctatcg cccgactgct ggcgaagggg ctaaactgcg
    13261 aaaccggcat taccgcgacg ccgtgcggcg tgtgcgataa ctgtcgtgaa atcgagcagg
    13321 ggcgctttgt cgatctgatt gaaatcgacg ccgcctcgcg caccaaagtt gaagataccc
    13381 gcgacctgct ggataacgtc cagtacgctc cggcgcgtgg tcgtttcaaa gtttatctga
    13441 tcgacgaagt gcatatgctg tcgcgccaca gctttaacgc actgttaaaa acccttgaag
    13501 agccgccgga gcacgttaag tttctgctgg cgacgaccga tccacagaaa ttgccggtga
    13561 cgattttgtc acgctgtctg caatttcatc tcaaggcgct ggatgtcgag caaattcgcc
    13621 atcagcttga gcacatcctc aatgaagaac atatcgctca cgagccgcgg gcgctgcaat
    13681 tgttggcgcg cgccgctgaa ggcagcctgc gagatgcctt aagtctgacc gaccaggcga
    13741 ttgccagcgg tgacggccag gtttcaaccc aggcggtcag tgcgatgctg ggtacgcttg
    13801 acgacgatca ggcgctgtcg ctggttgaag cgatggtcga gggcaacggc gagcgcgtaa
    13861 tggcactgat taatgaagcc gctgcccgtg gtatcgagtg ggaagcgttg ctggtggaaa
    13921 tgctcggctt gttgcatcgt attgcgatgg tacaactttc gcctgctgca cttggcaacg
    13981 acatggccgc catcgagctg cggatgcgtg aactggcgcg caccataccg ccgacggata
    14041 ttcagcttta ctatcagacg ctgttgattg gtcgcaaaga attaccgtat gcgccggacc
    14101 gccgcatggg cgttgagatg acgctgctgc gcgcgctggc gttccatccg cgtatgccgc
    14161 tgcctgagcc agaagtgcca cgccagtcct ttgcacccgt cgcgccaacg gcagtaatga
    14221 cgccaaccct ggtgccgccg caatcagcgc cgcagcaggc accgactgta ccgctcccgg
    14281 aaaccaccag ccaggtgctg gcggcgcgcc agcagttgca gcgcgtgcag ggagcaacca
    14341 aagcaaaaaa gagtgaaccg gcagccgcta cccgcgcgcg gccggtgaat aacgctgcgc
    14401 tggaaagact ggcttcggtc accgatcgcg ttcaggcgcg tccggtgcca tcggcgctgg
    14461 aaaaagcgcc agccaaaaaa gaagcgtatc gctggaaggc gaccactccg gtgatgcagc
    14521 aaaaagaagt ggtcgccacg ccgaaggcgc tgaaaaaagc gctggaacat gaaaaaacgc
    14581 cggaactggc ggtgaagcta gcggcagaag ccattgagcg cgacccgtgg gcggcacagg
    14641 tgagccaact ttcgctacca aaactggtcg aacaggtggc gttaaatgcc tggaaagagg
    14701 agagcgacaa cgcagtatgt ctgcatttgc gctcctctca gcggcatttg aacaaccgcg
    14761 gtgcacagca aaaactggct gaagcgttga gcatgttaaa aggttcaacg gttgaactga
    14821 ctatcgttga agatgataat cccgcggtgc gtacgccgct ggagtggcgt caggcgatat
    14881 acgaagaaaa acttgcgcag gcgcgcgagt ccattattgc ggataataat attcagaccc
    14941 tgcgtcggtt tttcgatgcg gagctggatg aagaaagtat ccgccccatt tgatcgtaag
    15001 cacagcttac gttcgtcatc cttaacgtga ttgagagaga aacctatgtt tggtaaaggc
    15061 ggtctgggta acctgatgaa gcaagcccag cagatgcaag aaaaaatgca gaaaatgcag
    15121 gaagagatcg cgcagctgga agttaccggc gaatctggcg caggtctggt aaaagtgacc
    15181 atcaacggtg cacacaactg ccgtcgcgta gagatcgacc cgagcctgct ggaagacgac
    15241 aaagagatgc tggaagacct ggtggctgca gcattcaacg acgcagcacg tcgtattgaa
    15301 gaaacgcaga aagaaaaaat ggcctctgta tcctccggaa tgcagctgcc gcctggcttt
    15361 aagatgccgt tctgatgcaa accagcccgc tgttaacaca gcttatggaa gcactgcgct
    15421 gtctgccggg cgttggcccg aagtcggcgc agcgtatggc gttcacgctg cttcagcgcg
    15481 atcgtagcgg cgggatgcgt ctggcgcagg cgctcacccg ggcgatgtcg gaaatcggcc
    15541 actgcgccga ttgccgcact ttcaccgaac aggaagtctg taacatctgt tcgaatccgc
    15601 gtcgtcagga aaacggtcaa atctgtgtgg tggagagtcc ggcggacatc tacgccattg
    15661 agcagacggg gcagttttct ggtcgttatt ttgtgttgat ggggcatctg tcaccgctgg
    15721 acggcatcgg tccggatgat atcgggcttg atcgtctgga acagcgtctg gcagaggaaa
    15781 aaatcactga agtgatcctc gccaccaatc ccacggttga aggtgaagct accgctaact
    15841 acattgccga gctttgcgcg caatatgacg tggaagccag ccgaatcgct catggcgtac
    15901 cggttggcgg cgaactggaa atggtcgatg gcaccacgct gtcacactcc ctcgccgggc
    15961 gtcataagat tcgtttttaa gcaaacgaga gcaggagcac ctgctctcgc ttgaaattat
    16021 tctcccttgt ccccatctct ccctcatcct gtttttaacc ttaaaatggc attattgagg
    16081 tagacctaca tgaaaggaca agaaactcgt ggttttcagt cagaagtgaa acagcttctg
    16141 cacctgatga tccattctct ctattccaat aaagaaatct tcctgcgtga gcttatctct
    16201 aacgcctccg atgcggcgga caagctgcgt ttccgtgcgc tatctaaccc ggacctgtac
    16261 gaaggtgatg gcgaactgcg cgttcgtgtc tctttcgata aagacaagcg cacgctgacc
    16321 atctccgata acggcgtggg gatgacccgc gacgaagtga ttgaccatct ggggactatc
    16381 gctaaatccg gtaccaaatc attcctcgaa tccctgggtt ctgaccaagc gaaagacagc
    16441 cagctgatcg gtcagtttgg tgttggtttc tactctgcgt ttatcgtggc cgacaaagtg
    16501 accgtgcgta ctcgcgcggc aggcgaaaaa ccagaaaacg gcgtcttctg ggaatcggct
    16561 ggcgaaggtg aatataccgt tgccgatatc accaaagaag atcgtggtac tgaaatcacc
    16621 ctgcatctgc gtgaaggcga agacgagttc ctcgatgact ggcgcgtgcg ttccatcatc
    16681 agcaaatact ccgaccatat cgcgctgccg gtagagatcg aaaaacgcga agagaaagat
    16741 ggcgaaaccg ttatctcctg ggcaaaaatc aacaaagcgc aggcgctgtg gactcgtaac
    16801 aagtcggaaa tcaccgatga agagtataaa gagttctaca aacacatcgc ccacgacttt
    16861 aacgatccgc tgacctggag ccacaaccgc gttgaaggta agcaggagta caccagcctg
    16921 ctgtacatcc cgtcccaggc tccgtgggat atgtggaacc gtgatcataa acacggcctg
    16981 aaactgtacg ttcaacgcgt gtttatcatg gacgacgctg aacagttcat gccgaactat
    17041 ctgcgcttcg tgcgtggtct gattgactcc agcgatctgc cgctgaacgt ttcccgtgaa
    17101 atcctccagg acagcacggt aacgcgtaac ctgcgcaatg cgctgaccaa gcgtgtgctg
    17161 caaatgctgg aaaaactggc gaaagacgac gcggaaaaat accagacctt ctggcaacag
    17221 tttggcctgg tactgaaaga aggtccggcg gaagatttcg ctaaccagga agcgatcgcc
    17281 aaactgctgc gttttgcttc tacttatacc gattcttctg cgcagaccgt gtctctggaa
    17341 gactacgttt cccgcatgaa agaagggcag gagaaaatct actacatcac tgcagacagc
    17401 tatgcggcag cgaagagcag cccgcacctg gaactgctgc gtaagaaagg catcgaagtt
    17461 ctgctgcttt ccgaccgcat cgatgagtgg atgatgaact atctgactga gttcgacggt
    17521 aaaccgttcc agtcggtgtc taaagttgac gagtcgctgg aaaaactggc tgacgaagtt
    17581 gatgagagcg cgaaagaagc ggagaaagca ctgactccgt tcatcgaccg cgtgaaagct
    17641 ctgctcggcg agcgcgtgaa agatgtccgt ctgactcacc gtctgaccga tacgccagcg
    17701 atcgtttcga ccgacgcgga cgaaatgagc actcagatgg cgaaactgtt cgctgcggcg
    17761 ggccagaaag tgccagaagt gaaatacatc ttcgaactga acccggatca cgtactggtg
    17821 aaacgtgcgg cagatactga agatgaagcc aagttcagcg agtgggtaga actgctgctg
    17881 gatcaggcgc tgctggcaga acgcggcacg ctggaagatc cgaacctgtt tattcgtcgt
    17941 atgaatcagc tgctggtttc ctgatgtcat gccggatgac ctttgtgtca tccggcattt
    18001 ttcttttcat catctgtact ttccgcaaat tatctcgcca ttaaccgttt cagcctcagg
    18061 tgcctttctt gaggcaatcg cctgttggtg gtatcgttta tcgctttttc aaaaaaatcg
    18121 acacatttta aggggatttt cgcaatgcgt atcattctgc ttggcgctcc gggcgcgggg
    18181 aaagggactc aggctcagtt catcatggag aaatatggta ttccgcaaat ctccactggc
    18241 gatatgctgc gtgctgcggt caaatctggc tccgagctgg gtaaacaagc aaaagacatt
    18301 atggatgctg gcaaactggt caccgacgaa ctggtgatcg cgctggttaa agagcgcatt
    18361 gctcaggaag actgccgtaa tggtttcctg ttggacggct tcccgcgtac cattccgcag
    18421 gcagacgcga tgaaagaagc gggcatcaat gttgattacg ttctggaatt cgacgtaccg
    18481 gacgaactga ttgttgatcg tatcgtaggc cgccgcgttc atgcgccgtc tggtcgtgtt
    18541 tatcacgtta aattcaatcc gccgaaagta gaaggcaaag acgacgttac cggtgaagaa
    18601 ctgactaccc gtaaagacga tcaggaagaa accgtgcgta aacgtctggt tgaataccat
    18661 cagatgactg caccgctgat cggctactac tccaaagaag cggaagcggg taacaccaaa
    18721 tacgcgaaag ttgacggtac caagcaggtt gctgaagttc gcgctgatct ggaaaaaatc
    18781 ctcggctaat caagccgcag cagatctgct ttcgggcagg tctgcttatc cacctcaggc
    18841 aatacctctc acagcaatta gttcttcttc ctcacttttc cgctacaatt atcaacaagt
    18901 tgaatcgata agaggcggta atgcgtcaga ctaaaaccgg tatcctgctg gcaaacctgg
    18961 gtacgcccga tgcccccaca cctgaagcgg taaaacgcta tctgaaacaa tttttaagcg
    19021 acagacgcgt ggttgatacc tcacggttgt tatggtggcc gttgctgcgc ggcgtgattt
    19081 tgccgctgcg ctcgccgcgt gtggcgaagc tgtacgcctc tgtctggatg gaagacggct
    19141 cgccgctgat ggtttacagc cgccagcaac agcaggcgtt ggcacaacgt ttaccggata
    19201 cgccagtggc gctgggcatg agctacggct cgccatcact ggaaagctcc gtcgatgaac
    19261 tcctggcaga gcatgtagat catattgtgg tgctgccgct ttatccgcaa ttctcctgtt
    19321 ctacggtcgg tgcggtatgg gatgaactgg cacgcattct ggcgcgcaaa cgtagcattc
    19381 cggggatatc gtttattcgt gattacgccg ataaccacga ttacattaat gcactggcga
    19441 acagcgtacg cgcttctttt gccaaacatg gcgaaccgga tctgctgctg ctctcttatc
    19501 atggcattcc ccagcgttat gcagatgaag gcgatgatta cccgcaacgt tgccgcacaa
    19561 cgactcgcga actggcttcc gcactgggga tggtaccgga aaaagtgatg atgacctttc
    19621 agtcgcgctt tggccgggaa ccctggctga tgccttatac cgacgaaacg ctgaaaatgc
    19681 tcggagaaaa aggcgtaggt catatacagg tgatgtgccc gggctttgct gcggattgtc
    19741 tggagacgct ggaagagatt gccgagcaaa accgtgaggt cttcctcggt gcgggcggga
    19801 aaaaatatga atatattcca gcgcttaatg ccacgccgga acatatcgaa atgatggcta
    19861 atcttgttgc cgcgtatcgc taaagctgag cggtaaagaa ctgagcgccg tcgcgaagag
    19921 cctcgtcggc ggttttcatc atccgtgaat aatgcaaaaa ggcatgcagc gtacctgggt
    19981 agagtttgaa ctcacagggc tgctgatgcg ccgctaacgt ctagtaaagc agacggctgt
    20041 catccagcag cggatcgaac tccgccccgg caataaaaca gggcggaact tcgcgagtga
    20101 gatcattatt aaacagacag taatacggtg actcgcggtc cgcgtcgttg cttaaatacg
    20161 cctcttcata catctgcaaa tcctgttgcg ttaagccatc ccagacaccg cccaacagac
    20221 gacgagtcac ggaatcccgt aatccgtaaa gcccatacca cagcaaaaca cccgcaattt
    20281 taccgcaatc gatctgttta tcacgcaacc acaacgcact ggcgagcgcc agcatggcac
    20341 ctgcggaatc accggcaaag ccaatgcggg acatattgat ttgataatcc tccgcctgct
    20401 ggtggaaata acaacaagca gccacaattt cctctatcgc ttgtggaaaa cgcgcttcag
    20461 gtgaaagggt gtaattaata ccaatcaccg tacattggct gtagcttgcc agcaggcgca
    20521 tgatgcgatc gtgggtatcg agattgccga gaataaaacc gcctccatgc aaataaaata
    20581 gcgtcgccgg gctatctggc tgcggacaaa agagacgtgt ttccacctgc ccatattttg
    20641 ttggaaccat gtaagctctg gttgccattt ctggagcgcc cgcattccag aatcggcgct
    20701 caagcgtgta atactgtcgt tgctcagcaa tcgttcccgt tgcgggccag gacggtaaat
    20761 ccggctgaag agtattcaca acggtcttca tttcagcaga aataaggtcc agaacaggta
    20821 gtttgttttc cggcttcata caaaaactcc tttcaaatta cgtcattgta aggaaccact
    20881 gccatgaaaa tgcgatcccg cctgctgata ttgaaactgg ctgcgtctcg cgcgctcccg
    20941 tcagattgtg ttaacattcg ccgctcagtt aaccacccgt aaaaacaacc atgaaatttc
    21001 ccggtaaacg taaatccaaa cattacttcc ctgtaaacgt aaatccaaac attacttccc
    21061 tgtaaatgca cgcgatccgc tgcttcagca attccagcca gaaaacgaaa ccagcgccgc
    21121 ctgggtcgta ggtattgacc agacgctggt cgatattgaa gcgaaagtgg atgatgaatt
    21181 cattgagcgt tatggattaa gcgccgggca ttcactggtg attgaggatg acgtagccga
    21241 agtgctttat caggagctaa aacagaaaaa cctgattacc catcagtttg cgggtggcac
    21301 cattggtaac accatgcaca actactcggt gctcgcggac gaccgttcgg tgctgctggg
    21361 cgtcatgtgc agcaatattg aaattggcag ttatgcctat cgttacctgt gtaacacttc
    21421 cagccgtacc gatcttaact atctacaagg cgtggatggt ccgattggtc gttgctttac
    21481 gctgattggc gagtccgggg aacgtacctt tgctatcagt ccaggccaca tgaaccagct
    21541 gcgggctgaa agcattccgg aagatgtgat tgccggagcc tcggcactgg ttctcacctc
    21601 atatctggtg cgttgcaagc cgggtgaacc catgccggaa gcaaccatga aagccattga
    21661 gtacgcgaag aaatataacg taccggtggt gctgacgctg ggcaccaagt ttgtcattgc
    21721 cgagaatccg cagtggtggc agcaattcct caaagaccac gtttctatcc ttgcgatgaa
    21781 cgaagatgaa gccgaagcgt tgaccggaga aagcgatccg ttgttggcat ctgacaaggc
    21841 gctggactgg gtagagctgg tgctgtgcac cgccgggcca atcggcttgt atatggcggg
    21901 ctttaccgaa gacgaagcga aacgtaaaac ccagcatccg ctgctgccgg gcgctatagc
    21961 ggaattcaac cagtatgagt ttagccgcgc catgcgccac aaggattgcc agaatccgct
    22021 gcgtgtctat tcgcacattg cgccgtacat gggcgggccg gaaaaaatca tgaacactaa
    22081 tggagcgggg gatggcgcat tggcagcgtt gctgcatgac attaccgcca acagctacca
    22141 tcgtagcaac gtaccaaact ccagcaaaca taaattcacc tggttaactt attcatcgtt
    22201 agcgcaggtg tgtaaatatg ctaaccgtgt gagctatcag gtactgaacc agcattcacc
    22261 tcgtttaacg cgcggcttgc cggagcgtga agacagccag gaagagtctt actgggatcg
    22321 ttaagttatc gtcggttcgt aggccagata aggcgttcac gccgcatctg gcatttggct
    22381 ctcgatgcct gatgcgacgc tggcgcgtct tatcatgcct acatattttt catattttac
    22441 atccggcaac caccgtttac cccgtcacca cttcacccgc cggtggcgtt tccagcagtt
    22501 ccagcatagt acgggcaatt tcacgttcac ccatcactac ctgattcgcg ccacgttcgg
    22561 tgatatacgt cacttcatcg tcataatggg cgcgggcgat aatctcaata tccgggtttt
    22621 tcgcgcgggc agacgccaca atctcacccg cttcataacc gttggggatc gtcaggatca
    22681 gccattttgc acattccaga tgtgccagtt gcataatttc ttcgttcgct gcattgccca
    22741 atacagcgcg gaccccacgc tctcgcagct catcaacacg ggttcgtgac gtctcaatca
    22801 ccaccagcgg aatatcagag gcgagcaatt tctcccccag caggctgcct acacgaccgt
    22861 aacccaccaa tagcgcatgg ttgcaaatat ccactgggat ctgtttctct tcttcgattg
    22921 cctcttccag cgtctgctct tccagcgttt cggtcttcgc cagatatttc tccagtagtg
    22981 cgaacagtac cgggttgagc ataatcgaca ggatcgcccc tgccagtacc aggttttgtc
    23041 cggcctgcgg cagtaaattc aatgccattc ccagtcccgc caggataaac gcgaactcac
    23101 caatctgcgc caggctggcg gcgatggtta atgccgtacg ttgggagtga ccaaacagac
    23161 ggacaaggaa cagggccgcg accgatttac caaacagaat aattgccagc gtcgccagca
    23221 ctgccagcgg ttgctgaatc agaattaacg gatcaaacaa catcccgact gagacaaaaa
    23281 acagcaccgc aaacgcgtcg cgcaatggca acgtatcgtg ggcggcacgg tgactcaatt
    23341 cagactcgtt cagtaccatc ccggcaaaga acgcaccgag tgcaaaggag acatcaaaca
    23401 gctctaccgc accaaaggca atccctaacg ccagcgccag cactgacagg gtaaacagct
    23461 cgcgagaacc ggttgccgcg ctgcgtgcca taatccacgg caccagacgg cgacctacca
    23521 gcatcataat ggcgataaat gcgatcacct tgccgatggt gatccccata tcgactgcaa
    23581 gagtggcaaa gcccacatcg ccctgttcca tcattcctgc cactgcgggc agcaacacca
    23641 gcgtcagaac catcaccagg tcttccacaa tcaaccaacc gatggcgatt tgcccacgct
    23701 gactgtctat taattgccgt tcttcaagtg cgcgcagtaa caccacggta ctggcggtgg
    23761 aaagacataa gccgaacacg ataccggtca ttaacgacca gcccagcacg gcggagagcg
    23821 ccatacccag cagcgtcgcc acggctatct gggcgatcgc gccgggaatg gcgatggact
    23881 ttaccgccat caaatccttg agcgaaaaat gcaaaccgac gccgaacatc agcaaaataa
    23941 cgcccagttc agccagttcc ggcgcaagct tggtatcggc aacaaagccc ggagtgaatg
    24001 gtcctgccag cacacccgct aacagatatc ccaccagagg agaaatacgt agtttattgg
    24061 ccagcatgcc gaggataaag gcgagcacaa ggccgccaac aatggtggtg ataagcgggg
    24121 tggcgtgatg cattccgtct ccttttcctg gtggttattg tccatttttg gctgggaaaa
    24181 ccaaaattac aggtaatagt ttatgacaat ttcattgatg atgtttatga ataattgttg
    24241 aattttgcag aaaaatggaa ttagctgcaa aaaaagcacg gttcggaaaa cagaaggcgg
    24301 tttaacagag gaaaggctta tggcgactgt aaaacgatgc agccaaagtt tgcctttggc
    24361 tgcaatgaaa tcagtcttta tgccggttat caggcaggaa tatggtcaac atccccaata
    24421 gtggcaggaa agcacagatt ttatagacta actcgatgct ggtgtgatcg gcgataagcc
    24481 ccagaactgc cgctcccaga cctcccatgc cgaaagcaaa accgaaaaag agtccagaaa
    24541 ccataccgat acgtcccgga agtagctcct gagcgtagac cagaatggca gagaatgccg
    24601 aagcgaggat aaatccaata atcaccgtta aaacccccgt ccagtgcagg cgggcgtagg
    24661 gtaaaatcag cgtaaacggc gcaacgccga ggatagagcc ccaaatcaca tatttccgtc
    24721 caattttatc ccctacaggc ccgccgatca ccgtacctgc cgcaacggca aacaggaagg
    24781 caaacagatg aagctgggca ttctggatag ataatccgaa tttttgcatc agataaaagg
    24841 tgtaatagct gctgatgctc gccatataga aatatttcga gaaaatgagg attaacagaa
    24901 tgctgactgc cagtaccact ttgttgcgcg gcagtggatt gattatcgtc gctttgggtt
    24961 ttcctttatt cattcggtgc tgtgccgagt accaacggct aatttgcgcc aacaccacga
    25021 tcgccagcag tgccgcaagc acaaaccagg caacgttgcc tttgccataa ggctcgataa
    25081 tcaccgccgc cagcaagggt cccagggaac tgccaaagtt gccgccgacc tgaaagatag
    25141 attgcgccag gccatgccgc ccgccggaag ccatacgggc tacgcgagaa gattccggat
    25201 gaaagaccga tgaaccggta ccgaccagcg ccgctgccag cagaactgcg ccaaaactgc
    25261 ccgccagcgc aagcagcacc agaccactta aggtaaagca catgccaatt ggcaacgacc
    25321 acggcatcgg atatttatcg gtccagtagc cgaccactgg ttgcagtagc gaagaggcga
    25381 gctggaaggt gagggttatc atgccaatct gcataaatgt cagagaaaat tctgactgaa
    25441 gcagcggata aatcgccaga atcagcgatt ggatcatgtc gttcagcaga tgtgagaggc
    25501 tgatagcacc taaaatacca aacgatgttc gggccttggt cgttgacgca gccgcgcccg
    25561 ccacaggctg ggtttgttca ctcattgcca taggaaagtc actttttcag ggttgcgatg
    25621 taaagaatga tcttatttgt gattattacc cgactaacat acctgtatgc gtcgtctgaa
    25681 ggaagtctca acgccgaata cagaatttct aatctggatg cagatttatc ttcaccggac
    25741 gcagacttgt ctatgatgtt gcgtcatact atttttcaac acgttgaaat caggtcaggg
    25801 agagaagtat gaaattattg cagcggggcg tggcgttagc gctgttaacc acatttacac
    25861 tggcgagtga aactgctctg gcgtatgagc aggataaaac ctacaaaatt acagttctgc
    25921 ataccaatga tcatcatggg catttttggc gcaatgaata tggcgaatat ggtctggcgg
    25981 cgcaaaaaac gctggtggat ggtatccgca aagaggttgc ggctgaaggc ggtagcgtgc
    26041 tgctactttc cggtggcgac attaacactg gcgtgcccga gtctgactta caggatgccg
    26101 aacctgattt tcgcggtatg aatctggtgg gctatgacgc gatggcgatc ggtaatcatg
    26161 aatttgataa tccgctcacc gtattacgcc agcaggaaaa gtgggccaag ttcccgttgc
    26221 tttccgcgaa tatctaccag aaaagtactg gcgagcgcct gtttaaaccg tgggcgctgt
    26281 ttaagcgtca ggatctgaaa attgccgtta ttgggctgac aaccgatgac acagcaaaaa
    26341 ttggtaacct ggaatacttc actgatatcg aatttcgtaa gcccgccgat gaagcgaagc
    26401 tggtgattca ggagctgcaa cagacagaaa agccagacat tattatcgcg gcgacccata
    26461 tggggcatta cgataatggt gagcacggct ctaacgcacc gggcgatgtg gagatggcac
    26521 gcgcgctgcc tgccggatcg ctggcgatga tcgtcggtgg tcactcgcaa gatccggtct
    26581 gcatggcggc agaaaacaaa aaacaggtcg attacgtgcc gggtacgcca tgcaaaccag
    26641 atcaacaaaa cggcatctgg attgtgcagg cgcatgagtg gggcaaatac gtgggacggg
    26701 ctgattttga gtttcgtaat ggcgaaatga aaatggttaa ctaccagctg attccggtga
    26761 acctgaagaa gaaagtgacc tgggaagac
//
LOCUS       DAJTSF010000053        26564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000053 DAJTSF010000000
VERSION     DAJTSF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.053"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            348..641
                     /locus_tag="OO335_002320"
     CDS             348..641
                     /locus_tag="OO335_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6002685.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="OO335_002321"
     CDS             713..1372
                     /locus_tag="OO335_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6002686.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="OO335_002322"
     CDS             1464..1910
                     /locus_tag="OO335_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR6002687.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="OO335_002323"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="OO335_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3390..3809
                     /locus_tag="OO335_002324"
     CDS             3390..3809
                     /locus_tag="OO335_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6002688.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3809..5077
                     /gene="umuC"
                     /locus_tag="OO335_002325"
     CDS             3809..5077
                     /gene="umuC"
                     /locus_tag="OO335_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6002689.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="OO335_002326"
     CDS             complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="OO335_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6002690.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="OO335_002327"
     CDS             complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="OO335_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6002691.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7561..8280
                     /gene="fadR"
                     /locus_tag="OO335_002328"
     CDS             7561..8280
                     /gene="fadR"
                     /locus_tag="OO335_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6002692.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8332..9864)
                     /locus_tag="OO335_002329"
     CDS             complement(8332..9864)
                     /locus_tag="OO335_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6002693.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10194..11492
                     /gene="dadA"
                     /locus_tag="OO335_002330"
     CDS             10194..11492
                     /gene="dadA"
                     /locus_tag="OO335_002330"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6002694.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11502..12572
                     /gene="dadX"
                     /locus_tag="OO335_002331"
     CDS             11502..12572
                     /gene="dadX"
                     /locus_tag="OO335_002331"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR6002695.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12740..14154)
                     /locus_tag="OO335_002332"
                     /pseudo
     CDS             complement(12740..14154)
                     /locus_tag="OO335_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14765..16501)
                     /locus_tag="OO335_002333"
     CDS             complement(14765..16501)
                     /locus_tag="OO335_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6002696.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="OO335_002334"
     CDS             complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="OO335_002334"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6002697.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17610..18221
                     /gene="emtA"
                     /locus_tag="OO335_002335"
     CDS             17610..18221
                     /gene="emtA"
                     /locus_tag="OO335_002335"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6002698.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18223..18957)
                     /locus_tag="OO335_002336"
     CDS             complement(18223..18957)
                     /locus_tag="OO335_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6002699.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19158..19412
                     /locus_tag="OO335_002337"
     CDS             19158..19412
                     /locus_tag="OO335_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6002700.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19462..20922)
                     /locus_tag="OO335_002338"
     CDS             complement(19462..20922)
                     /locus_tag="OO335_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6002701.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20859..21416)
                     /locus_tag="OO335_002339"
     CDS             complement(20859..21416)
                     /locus_tag="OO335_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR6002702.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21442..22296)
                     /locus_tag="OO335_002340"
     CDS             complement(21442..22296)
                     /locus_tag="OO335_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR6002703.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22296..23105)
                     /locus_tag="OO335_002341"
     CDS             complement(22296..23105)
                     /locus_tag="OO335_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR6002704.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23115..23873)
                     /locus_tag="OO335_002342"
     CDS             complement(23115..23873)
                     /locus_tag="OO335_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6002705.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23870..24850)
                     /locus_tag="OO335_002343"
     CDS             complement(23870..24850)
                     /locus_tag="OO335_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR6002706.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24850..25872)
                     /locus_tag="OO335_002344"
     CDS             complement(24850..25872)
                     /locus_tag="OO335_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR6002707.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6674 a         6472 c         6855 g         6563 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgcga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg gcgggatgcg
     3121 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
     3181 gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc
     3241 gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
     3301 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac cactgtatat
     3361 aaaaacagta taacttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
     3421 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
     3481 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
     3541 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
     3601 tactgattgt cgatagtgct attaccgcca gccatggtga tattgtcatc gctgctgttg
     3661 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
     3721 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
     3781 tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa acgcgtttta
     3841 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
     3901 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gcgttaaaat
     3961 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
     4021 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
     4081 atcgccccgc gtcgagattt acagtattga tgaagcattc tgcgatctga caggtgtgcg
     4141 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     4201 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ttggctaagc ttgccaatca
     4261 tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg gatttatcaa atctggaacg
     4321 ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc tgggggattg gacggcggat
     4381 cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc gatttggcgg atacagatat
     4441 ccggtttatc cgtaaacatt ttaatgtcat gctcgaaaga acggtgcgtg aactgcgcgg
     4501 cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg
     4561 ctcgtttggt gaacgcatca cggattatac gtcgatgcgg caggccattt gtagttacgc
     4621 tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat tgtcggttta tctccacatt
     4681 tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagcg cgtcggtaaa
     4741 actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga
     4801 tgccatctgg caagcgggcc atcgctacca aaaagcgggc gtgatgctgg gggatttctt
     4861 cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc ccgggagtga
     4921 gcaattgatg gcggtaatgg atactctgaa tgctaaagag ggcagaggaa cactctattt
     4981 tgccgggcag gggatccaac aacaatggca gatgaagcga gctatgcttt caccacgtta
     5041 tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg aaaaaagcgc
     5101 tcccgcagga gcgccgaatg gattagcgac cgaacagatc acgttttttc gctttaaacg
     5161 gctgggaaat caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc
     5221 actgcggcat ttccatacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga
     5281 caaacacttg cggcacccat ttatccagcg gcagccattc cgggaaacga accataaaat
     5341 cacaggtggc aaatggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca
     5401 caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg
     5461 gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa
     5521 tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca
     5581 gtgccagagc agtaaacgcc atcaacagcc acgcgccccg gccttgtgaa cattggttca
     5641 aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc
     5701 attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa ttggacaatt atcgggcgaa
     5761 aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc
     5821 atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag caagccgacg
     5881 agtgtcagta cgagggtgta aggcagggcc atccacacca tgcggccata agagaggcga
     5941 atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta
     6001 gcgacagagg gcagattggt accggtatta atggcgaccg ccagcagctc gtattgcttc
     6061 aacgtgatag cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg
     6121 aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag cgacagctga
     6181 gcatgttcag atgcctgcaa cacaaactga ataatcggcg aaaacagttg ttggtcgata
     6241 atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg
     6301 aaggctttac cgatagcatg ttcatcggtg acaccggtca atgatgttgc cagaataatg
     6361 actgacaaac caatcaagcc aacttccgcc agatgcaacg ccagcgcagt caccagccag
     6421 acgccaataa tcgcctggac aatcagacgg attttatcct gacgggtacg ctggtggcgg
     6481 ctttgatcgt caaactgttg caacacttcg cggactttct ccggcagcgt ttcaccgtag
     6541 ccaaaccaac gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccgga
     6601 acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg
     6661 atgatcaggt tctgtggttc gcccaccatg gtcattacgc cgcctaatgc ggtgccgaca
     6721 ccggcatgca tcatcaggct acgcagaaag ccacggaact gttccagaac cacgttgtaa
     6781 tgcttgtcga tatgactatc gtcttgcagg tcggtgtctt cggtacggga agaggctacg
     6841 cgatgataaa taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag
     6901 gcatcgagga acgcggagag gaacgctgcc gccacgcaaa aagagagcga cagcagcatt
     6961 ttggagcgaa tgcttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa
     7021 tagatacccg ccaccataaa catcaataac agcaagactt caagatttgc cgccacctct
     7081 tcacggacgt gttccgcgct ggtcatgccg atgaataccg cttcgatagc caacagacca
     7141 ccggggagta gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc
     7201 agcaaccagc ccgcgacgaa agggctgatg aggaaaatta acgggtttac gattaagaaa
     7261 atgatgaggg cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagcgcg
     7321 cggccccagg agatctccat gatggtttcc cttaccttac aaataatcaa tgatgttttt
     7381 atgtttaaac gcaaagctta acggtcaggc aggagtgagg caagtcttga tagtcaaggg
     7441 gaaagagatg cggaaaatga agccttgatc cctttttctt ctttttgtct gctatcagcg
     7501 tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact
     7561 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc
     7621 tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta
     7681 attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg
     7741 ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta
     7801 aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat
     7861 ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat
     7921 cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
     7981 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt
     8041 tacggtctga tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc
     8101 gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc
     8161 agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc
     8221 gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa
     8281 tcccttccgt ttaaagagca accccctcaa acgaggggtt ttttgttgtt tttacagatt
     8341 tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc
     8401 gagcatcaca tcaaatcccc acaggcgatg cacatgcttc aggacttctt tgcgcccccg
     8461 atccagcggt gcgcgattat gtggaatata acgcagcgtc agcgaacggt cgccgcgcaa
     8521 atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa
     8581 ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt
     8641 atgccgatca tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag
     8701 aaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag
     8761 ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca
     8821 aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta
     8881 ccacgggctg ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag
     8941 cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca
     9001 gaaggtcgca cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct
     9061 caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt
     9121 ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac
     9181 cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag
     9241 atactcttcg cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgcgggcg
     9301 tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac
     9361 ttcatcaacg ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata
     9421 atcgacaatc gaactggcgt cggtccaact gcggaataag caattgtttt taaagaaaga
     9481 gttatgtcca tagcaggcgt gtgccatcac cagcgcctgc atagtaatgg tgttctcttc
     9541 catcaagtaa gcgatacacg ggttagagtt aatgacgatt tcataggcca gcccttgctg
     9601 accgtgctta tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata
     9661 gttaattggc atgccgacgc tggaataggc atccatcatc tgttctgagg ttatcacttc
     9721 aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag
     9781 ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt
     9841 attcatagaa tcgatcgtcg ccatacgcgc acctcattgt tgtcggcgct ctctgtgtgg
     9901 agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc
     9961 tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg
    10021 ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga
    10081 tgtgagtcag ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca
    10141 agatgtaatt agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag
    10201 ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag
    10261 gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc agtgccgcga
    10321 atgccgggca aatctccccc ggatatgctg caccgtgggc agcaccaggt gtgcctttaa
    10381 aagcgattaa atggatgttc cagcgccatg cgccgctggc ggttcgtctc gacggtacgc
    10441 agtttcagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg
    10501 aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc
    10561 gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta
    10621 ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac
    10681 cgtatcagct gctggaatcc agccgcctgg cggaagtgga tcccgcgctg gcagaagtag
    10741 cgcacaaact gacgggcggc ctgcagttac ccaatgatga aaccggagac tgccagctgt
    10801 ttacccagaa tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc
    10861 ccgttgacca actgctttgc gacggcgagc aaatctacgg cgtgaagttt ggcgatgaag
    10921 tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag
    10981 gcattgttga tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc
    11041 aggaagatgg tgcgccagta tccaccattc ttgatgaaac ctacaaaatc gccattaccc
    11101 gtttcgataa ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc
    11161 tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg
    11221 gtcatgtcga gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc
    11281 cggttgtcgg gagtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg
    11341 gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc
    11401 cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac
    11461 gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg
    11521 ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc
    11581 acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct
    11641 ggagcgcgct cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt
    11701 tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg
    11761 atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca
    11821 aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg
    11881 ggatgaatcg gttgggcttc cagtccgatc gcgtgcttac cgtctggcag cagttgcggg
    11941 caatggcgaa tgttggcgaa atgaccctga tgtcgcattt tgctgaagcg gaacatcctg
    12001 atggaatttc cggcgcgatg gcgcgtattg agcaggcggc ggaggggctg gagtgtcggc
    12061 gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc
    12121 ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata
    12181 ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag
    12241 cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca
    12301 ttgtcgccgc aggatacgcc gacggttatc cgcgccatgc gcctaccggt gctcctgttt
    12361 tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctggcggttg
    12421 atttaacgcc ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga
    12481 tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcac
    12541 tggcgttacg cgtcccggtt gtgacagtgt aacttgttgt aagccggatc ggaggcagcg
    12601 tcttctggtt gcgaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga
    12661 cagagccatt gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg
    12721 aactcgccgc acaggcgttc tacataaaac gcttacgctt cattgttgac tcggagtcga
    12781 cttcagataa atcacgcttc acccttgatg cagattcggg ttcatgttca gattgttgat
    12841 taacaggctg cgagtcctgt tgtctttcca gactcaacag gcccaactgt tgatccagga
    12901 gctccaggtc ctcctgtacc ttcacgcgct ccaggagttc cgggtcatcc aggaactcca
    12961 gatccataag gtcctccagc tcctcgagtc cttccaggtc aacctagcct tccttgtgca
    13021 aaaagtccaa aaggtccttc aggccctgct gttttttcag gccctcgacg gctttgagca
    13081 gcgtcggtaa attgtctttg tcccacgggt taccattaca gtcaaacacc ttcaggctct
    13141 ggggctgcgg cacctgtacc gttttaaact tgttttctcc acagtagtaa attattagtt
    13201 tgggcggcag ccgtggtgaa gagatcaaat cgttactaga acagtcaaat actttcaggc
    13261 gagtcaagtt ctccagcatc tcatcaggta ttgagttaat attgttgccg gcacaggata
    13321 gtttatacag gtactggggc atcgacggcg gtaaaaattg caagaagttt tcagaacagt
    13381 taatcactct caggttagtc gacagctcag gcagccagat aatactgttt tcatagcact
    13441 tcagtaccat cagttttgag ggctgcacct tcggtaacaa gatcaagacg ttatctgtac
    13501 agtcaagcac cagcagccac tttggcatca ccttcggtaa cgcggtcaac acgtttttac
    13561 tgcagtaaaa ttcaatcaga tgtggaggca aattctcagg gagtgaagtc aatcccaggt
    13621 tgctaagatt gagcatactc cgtcttgtaa tgagacattc cttcattctg cgcaatgctt
    13681 ctgcccggtt ttcaatccgg cttgctggca ggttttcttc caggatattt tccttgattt
    13741 cttccagact ttctttcagg tcttcttggg gaatttctga ctgtttgttt tgctggtttt
    13801 cttccaggat ttcgtacagg tttttttgca ggttttcttg caaaccatcc tgctgtatag
    13861 cccattcgca ccatttggcg accatcatga actcgttatg ttgctgattc agacgagtcc
    13921 ccaacgtgat gatggtattg ttgtccacct gtatgttcac tttgcccgcg atactgaggt
    13981 caatgatctg ccagggggca tcaccccagc tgaatttttg cgggctgaga tacttcatgt
    14041 tctgcaaaat ttgattcttg agaagagccg ccctggcggg cgtcgtacgc ctgggttaac
    14101 ggaagatggg ggaatggcgg gggcgaatgg gcagatgtct ttaaagctag ccataatgct
    14161 acgtttcctt gaaataaata tcctctggca gcgccggaga cgtgaatgtg gtcaccgcaa
    14221 aggagccaca ggacgtcatt tgcgctgata tcactactca tcgttgactt ttttcctggt
    14281 cagaaatgcc gtgtcgggtc tggtacatgc tgtattacgg cctaggtagc tctgacgtat
    14341 taaagaaaat acgagagtga acgacccgga aataatcgtt attaagcaaa ggaataacta
    14401 taagaaatcg ctcatgcatc cggtgctaca ggcggatgaa acgcctattg tgtgggctat
    14461 gtcagtgcgg caggttgcgt gggggaatat gttcaggcga tgtcaggcag gggcccctga
    14521 tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa aaagccctta
    14581 cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa tggtttgtca
    14641 tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg aaatgacaag
    14701 tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag atgctgcatt
    14761 aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac acttcattat
    14821 cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca ccgacaaccg
    14881 gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta tcacgatatt
    14941 ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg ctggcttcga
    15001 gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg ctgaacagtt
    15061 taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg ccttcgcgca
    15121 gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct gcaatacgcg
    15181 tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta tcagcactca
    15241 attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca acgcgtgaca
    15301 ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac gagagtgatg
    15361 ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag aacagtcgcg
    15421 cattctccag ccccgccatc atcgggaaca cggctaggat gattggcacc gcgccgcgta
    15481 atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa gggagcaatc
    15541 cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa atgagcgccg
    15601 gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc aggaacatgg
    15661 cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag cggttgcgaa
    15721 tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg ctgccttcca
    15781 gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat ggatataatc
    15841 cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg ccaagaccga
    15901 tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa ctgacactgc
    15961 tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc attggatcat
    16021 tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc ttaccaccca
    16081 gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt aataagcctt
    16141 caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca gaggtgataa
    16201 gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag gaactggcct
    16261 gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt accatgtagg
    16321 cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc agcatgccga
    16381 tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa ctaagtaata
    16441 tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt gtggcatcca
    16501 atggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat tagcggcaga
    16561 gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac aggatgaccg
    16621 ctaatcgtca actgagcgcc ttcccgggtg ttattcagaa tggcatgtgc gcccaacggc
    16681 agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg aatcgacagg
    16741 cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata atcattgggc
    16801 gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat tcccgcatga
    16861 tagagctgca acagcatacg ttcgacgcgg aaagggtgca cgttaatatc ttccagcacc
    16921 agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat cagcatcgca
    16981 agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc ttgccattca
    17041 atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa ggcgttcagt
    17101 tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac attgccttgc
    17161 gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag taacgggtca
    17221 tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg actggcaccg
    17281 taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc aagggaattg
    17341 agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg ggcaataacc
    17401 tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg cagcgcggcg
    17461 tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat ggcaattcct
    17521 tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg tcacctcaac
    17581 ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc tttttgattg
    17641 tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg aacgcgaaag
    17701 ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt gcagcctggg
    17761 gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt aatccgaacg
    17821 cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc tccggacgtg
    17881 atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg aaaaatccag
    17941 agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc ccgctggcag
    18001 gcattgaaga tccgaaggta ctgcaatatg cgctggtggt gtcatacgct aacggggcag
    18061 gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa atcaacgatt
    18121 tggatgctga cgagttcctc gatcacgtag cgcgaaatca tcctgcgccg caggctccgc
    18181 gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc gcactttgtc
    18241 cgctttttcc cgggcttctc gctcgagaga gaaaataatc cgctgtaatt gccgctccac
    18301 cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg tttcattctt
    18361 gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt gaaaaacacc
    18421 ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta attcggcagg
    18481 ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca agcggaaacg
    18541 taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga gtggggcgga
    18601 gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta cggtaataaa
    18661 tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact cgactttcgc
    18721 accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agcacggcga tgttgtcttc
    18781 ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta ttttgctgat
    18841 cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca aatcgcgtaa
    18901 cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat gactcacgcg
    18961 gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac aaaacttaat
    19021 acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac gcccagcatc
    19081 gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga ccgattcatc
    19141 gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg atagccggca
    19201 ttatcggcaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg acctgtattc
    19261 tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc attgacggct
    19321 ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct atcctggtgc
    19381 tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat agcgatacag
    19441 atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga aattgcgacc
    19501 ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat tgaccgacgc
    19561 attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca ccgcaaaacc
    19621 ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct cttccgacag
    19681 cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg tctgcgttgg
    19741 aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca gagcgtagct
    19801 caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca gaccgctgta
    19861 atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt gcgagaggat
    19921 ggcatcactc acccggttat agtaaacgct gacctcaaat ccccagtcgc gcgtgaaggc
    19981 accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgcg ggttaacaat
    20041 cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg tcggaaagcg
    20101 tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt atttccccat
    20161 cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc tgccatcttt
    20221 ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg ccacgacatc
    20281 gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac gatcttcata
    20341 gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt tccagttaac
    20401 ggcaaacgac agcagatcgt tttcacgcac atcggctgcc agttgtaatc cggcaccgtc
    20461 gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag ccagcgagtt
    20521 gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca gggtaaaacg
    20581 gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact ctggccactg
    20641 ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt tttcaccatc
    20701 ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac cgagcttcac
    20761 aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc ctgcaatatc
    20821 attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac cgctgacttg
    20881 caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg cattgtcctg
    20941 actacggctc catccctggc gatatcccag acttgcttct tcagggtggt gatattaatg
    21001 gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga aacttcgact
    21061 gcccccagat tgttggtcag aatccgcgcc agatcgaggt tgccgtcata ggggacataa
    21121 atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg aacttttacc
    21181 tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac acttaatgcc
    21241 tgagcgacat tctgtttatc caaggccttc atggtgggtt gatcgagaac ggtggtcgtc
    21301 gtcgatgata ccggactgga ccatacggtc agcgtgtcgc tctcctgagc cgctacggcc
    21361 tgctgggtaa aggcaagtga taatgctgtg cagagataac gttttttgag tctcatagaa
    21421 taaattccgt ttaacaaaga attaaataga ggctgtgggt tgcagactga ctttaatgtc
    21481 agctggtgcc gcgtaataag gcgcggaggt aataaaaagg cgaatgccgc agtcgaggta
    21541 atttttgagt gtcgtcagat taattccgcc ggtcagagcc agcgtgcagt gaggagccag
    21601 cgacggcgct atttgcgcaa tttcagttgc ctgttgcgga ctaaatttgt cgagctgaag
    21661 tacgtctggt tgtgcgcgta acgcggcgat agcctctttc ggcgtgtcgg cttcaacaac
    21721 aattttcttc tcgggtgcgt ggcgacgtaa ctgagcgatt gcgcctgacc agccctgatt
    21781 gtcatgaaga aaatggcggt ggttggcaaa caataatatg gtttccgcac atccggcacg
    21841 atgaattagt cctccggcag ccagaattgc ctgcgaagcc agtaagcggg tgcccggaat
    21901 tgcttttcgg gtgcaggcga tattgccgtc aggataacgt tcacgaagca atgccagcat
    21961 ttgagcgaga taatcagaaa caccgcaact ccactccagc acattctgga ctgccttcca
    22021 tccctgatga agtgctgcgg cattaccctg cgcacggatt agccgttgac ccgcgttcgc
    22081 ttgtgaaccg tcgctgaccg cgtcatcaat agttaatccc agcgtagtta acatcttaca
    22141 cgcgacagaa acgccgctga cgcagccgcc ctgacggtga aaaaacgcta tatagccagg
    22201 ctggtgtcca atatttaatg ctcgcgtggt caggtcacct ccctggatat cttccagcag
    22261 taacgcatcg atttgtgcct gagaaaggaa aatcatcaca gtgcctcctg cggtacgcta
    22321 tcccaccaga tgagcgccca gtcgcggctg gcaggggcga gggcacgagc atcttgttgt
    22381 tgataccagt gatataaccg ctcacgctca tcgtcgttaa gcgcgccaag actccagctg
    22441 acgttctgct caaaacgttc ccaggtgctg ttatcctgtt ggcaattttg tccacggata
    22501 aaatcaacgt gggcataaat tcccatctga tacaggacgt tgagcgcaaa gatatagttg
    22561 ggtaattcga tcacttcccg accagccgcc cgctgaatgg ctggagagac gaacgaggtg
    22621 ctgaccagat gagttgtata gacgcgaagc cgtgcctgat tattcagttt gctcattgca
    22681 tggcgcatat cagccaccag agtggagcgg gaagccacgg caatatcgca gcgtggcagg
    22741 tcactccagt cctcttccca ggcgcgctgg atccagtgga cattgtcagc cttcagtgcg
    22801 gcggcgcggc gggcggcaac gttcagcatc ccctgactgt aatcaacgcc ataaaccgta
    22861 gtgagtttgt cagccagcgc cagcgatacg gtgcctggtc cgcagcccat atcgaataag
    22921 gtctgagcgc cttgcaggtc aattttagcg atcagttgtt gcaggtagct gtccgttggc
    22981 gaggcgcaat tttccgccat tttctccgcc cgttgatccc agtgatcggg cgttttttct
    23041 gtgcggtgag ccagtttgag ttgctgcaga taaaggtctg caaaatcaat atcatcaatg
    23101 agcatgattg ttccttagcg gctaattgca gagaggtgat gatgaatttg gtcggcagaa
    23161 acccgataca gggccgccag tttgttcgtt gttaactgtt cggtcggtaa gccttgtgtt
    23221 actcgtccat ctggctctac ttggataatg ctgtcggcaa tggcgttggc atggagcgta
    23281 tggtgagtcg acattaacat tgtcatgccg ttatttttga gctgcgccag cgtgtccaga
    23341 agttggatct gatgaccgaa atcgaggctg gaggcgggct catccagtaa caacaagcgc
    23401 ggttgttgga cgagtgcacg ggcgattaaa gccagctgac gttcaccgcc gctaagcgta
    23461 ttccagcgtc gtgtcgccag atgcaaaatg cccagctttt ccagttgctc cgtggctttc
    23521 aggcgttctt gcttaccggg aacggaaaaa gcgcctatgg tgggggccag gcccatcaac
    23581 accatatcca gcacactgaa agcaaaaata ccgtcgtgcg cctgcggcac ccaggcaata
    23641 gcctgtgcgc gctggcgatg gctgagtgtc tgcacagaga ggctatccag taggatctcg
    23701 ccttttaata acggtataac gccaagaatg gagcgcatga gtgtggtttt gccgcaacca
    23761 ttcgcgccaa gcagacaaca aattgttcct tgcgacaggg tgaaggacac gtcgcgtagc
    23821 actggatggc gggagtaacc cagagacgca tgacgaacgg caagcaaagt cactggcgac
    23881 ctccgcgtaa caacaaaagc aggaaaaacg gtgcgccaac aaaagcggtc agaatgccaa
    23941 gtgggatttc tgttgttcca atgctgcgcg ccagcgtatc ggtaagcagg agcagaatag
    24001 cgccagtaca cattgccata ggcaacagct gctgatggtt atgaccggtc aacaagcggg
    24061 caatgtgcgg aacgaccaga ccgatccaac caattatgcc cgctattgct accgtgctgg
    24121 cggtgattag tgtggcgcag acaatcaatc caaagcgcag acgcgtcacg ttcagtccca
    24181 gcgaacgggc ttcgtcgtcg gaaagcgtga gtaaattcat gcgccagcgg agaaaaaaca
    24241 ggggcaagga gcctatgaga atgatcggtg cagcatagca gagatcgcgt aaggtcacgg
    24301 tggacagtcc ccccagcaac cagaaggtaa ttgacggtaa ctgggtgtaa ggatcggcca
    24361 gcgttttaat tagcgatatc cccgctccgc acaacgttcc taacgcaatg cctaccagca
    24421 ccagggttaa gatcggatcg tggcgggtca cccgacgcgt aatcaaccag acgcccgcga
    24481 ccaccatcag cccgccgcaa aatgcgtaca gctggatgta aacaatgggt aatccccaca
    24541 ggattgccgc gcaggcacca agtccggcac cggcggagac gcctaaaata tccggtgaaa
    24601 ctaagggatt acgcaacatt ccctgatagg tggttccggc tcctgccagc gctgcgccca
    24661 gtaacagcgc agcaagaata cgcggcacgc ggatttgcca gaagacaatt tgttcctgca
    24721 cagactcatt ttcttgacga agaattagcg ccagcagttg ctgcatatcg agatgataag
    24781 ccccacttac ggcggcaaaa gtgatactga tagctgccag cagcaaaata catccgttta
    24841 cgattctcat tagctcgcca ccaatttttg atagtcggca tcactgagtg aacaatgcca
    24901 gaacagttgg gcgtaatgct gcatgtcact tttaaactgg cgattgatgg cgggatccag
    24961 ccaggcgtga agtctgcgta atcccagaag acggttgatt cccggcgggg catcgagcca
    25021 gccaaagggc aagccactta aaaataagat gcgttgttcc gcaacggctt tcacgccctg
    25081 ccagagagga tcacgacgaa taaaatctgc agtaacggcc tcctgaacca gaataatatc
    25141 cggctgccag cgcaggagat tttccatgga aacctgtgtc aggccgtggc gatcggctat
    25201 ttgcgcgacg ttgtgcaaac ctaataattc cgccgcctcg gtatgcaacg aaccctgtaa
    25261 gcccgtttcc agccctcggg gaccgcgcgc agcataaaag cggaggttag cggcgggtga
    25321 ggtggcgaat gcctgagctt caccaacgaa gtggctggcg agattggctt gttctgcggc
    25381 gcggtgctct tcgcctaacg ttttgccaag cgttgttaac tgttctgctg attgcgccag
    25441 ttttccgtta agcaataacc aggggatttg tgtctgttcg ctaacctgcc gtgcctggga
    25501 gatcaaggtt tcatccgtat tgccgcaatc aacgaccaaa tcgggatgta acgccataag
    25561 cccttccagc gatagtgtgc tggcgcgccc ggcgagtcgt cccagcctgg gtaactggcg
    25621 aatgtgctct ggcaatggaa ttaatgcctg acgggcaaaa tcaaaagagg aaaaaccgac
    25681 cattttttct ggcgcgacag ccagcaagag caaatcggcg ggcgcgcctg cgctgacgat
    25741 gcgtgtaatg ttttgggctt cgggaagaga ttgcgaagca aaacgattaa ctgtttgtga
    25801 aaaggacggt agagactgca ggagcagggt agatgcaagt gcctgcgcaa acgtccgtct
    25861 ggttatgggc atggtgaaca tcctgaaaag taaaatcgat atatactttc atatatatcg
    25921 ataaaaatca tgttcaatat caaagtttag ctattgaaaa catgaagatg tgacgacttc
    25981 cgcaagcctg ttgaccctct ctatcattaa aaatgtattt atcaggtggc taatattatt
    26041 taaatcattt aaggctaaat tatttaaata tgaatgacat tattgttttt ttatcgtcca
    26101 cggttacgcg acgttgaagt tacttgtttt ataatgagcg atttttgata tctcgccgag
    26161 aaaattatca gcattctctc gtttcagtgt cgtgaatata ctgttaacgg aggtgaaaag
    26221 aatgctgggg gttatacaaa acctatgttg ttgtcgtccg gattcaccgc ttgctgatag
    26281 taagcagcgt aaccagaagt taccgcaaca agactttgag gattttaaga acgacgttat
    26341 taatgtcatt aacaacaatg aagatattta ttttcttaga gagagtaatt tactgaatgc
    26401 gtttattcgg acgtgcaaaa aaagagatga taacagttac ggaaatatga tatttacgct
    26461 ttattttgat aacgatatct atgggaacag aattatcaaa gcatcgctga acggcggaga
    26521 gttgcagtta ttaagcacag ggtgtagcaa tgataacatt ctcc
//
LOCUS       DAJTSF010000054        25855 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000054 DAJTSF010000000
VERSION     DAJTSF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.054"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(95..1774)
                     /gene="bcsG"
                     /locus_tag="OO335_002345"
     CDS             complement(95..1774)
                     /gene="bcsG"
                     /locus_tag="OO335_002345"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6002708.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1771..1962)
                     /gene="bcsF"
                     /locus_tag="OO335_002346"
     CDS             complement(1771..1962)
                     /gene="bcsF"
                     /locus_tag="OO335_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6002709.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1959..3530)
                     /gene="bcsE"
                     /locus_tag="OO335_002347"
     CDS             complement(1959..3530)
                     /gene="bcsE"
                     /locus_tag="OO335_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6002710.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3803..3991
                     /locus_tag="OO335_002348"
     CDS             3803..3991
                     /locus_tag="OO335_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6002711.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4003..4755
                     /gene="bcsQ"
                     /locus_tag="OO335_002349"
     CDS             4003..4755
                     /gene="bcsQ"
                     /locus_tag="OO335_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6002712.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4752..5111
                     /locus_tag="OO335_002350"
     CDS             4752..5111
                     /locus_tag="OO335_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6002713.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5108..7369
                     /gene="bcsA"
                     /locus_tag="OO335_002351"
                     /pseudo
     CDS             5108..7369
                     /gene="bcsA"
                     /locus_tag="OO335_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7380..9745
                     /gene="bcsB"
                     /locus_tag="OO335_002352"
                     /pseudo
     CDS             7380..9745
                     /gene="bcsB"
                     /locus_tag="OO335_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
     gene            9752..10858
                     /gene="bcsZ"
                     /locus_tag="OO335_002353"
     CDS             9752..10858
                     /gene="bcsZ"
                     /locus_tag="OO335_002353"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6002714.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10935..11276
                     /locus_tag="OO335_002354"
     CDS             10935..11276
                     /locus_tag="OO335_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6002715.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11191..11442
                     /locus_tag="OO335_002355"
                     /pseudo
     CDS             11191..11442
                     /locus_tag="OO335_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            11524..13512
                     /gene="hmsP"
                     /locus_tag="OO335_002356"
     CDS             11524..13512
                     /gene="hmsP"
                     /locus_tag="OO335_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6002716.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            13701..14987
                     /gene="dctA"
                     /locus_tag="OO335_002357"
     CDS             13701..14987
                     /gene="dctA"
                     /locus_tag="OO335_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6002717.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15208..16704
                     /locus_tag="OO335_002358"
     CDS             15208..16704
                     /locus_tag="OO335_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6002718.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(16800..17729)
                     /gene="kdgK"
                     /locus_tag="OO335_002359"
     CDS             complement(16800..17729)
                     /gene="kdgK"
                     /locus_tag="OO335_002359"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6002719.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17961..18728
                     /gene="pdeH"
                     /locus_tag="OO335_002360"
     CDS             17961..18728
                     /gene="pdeH"
                     /locus_tag="OO335_002360"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6002720.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18798..20858
                     /locus_tag="OO335_002361"
     CDS             18798..20858
                     /locus_tag="OO335_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6002721.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21040..22362)
                     /locus_tag="OO335_002362"
     CDS             complement(21040..22362)
                     /locus_tag="OO335_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6002722.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22773..23786)
                     /gene="yhjD"
                     /locus_tag="OO335_002363"
     CDS             complement(22773..23786)
                     /gene="yhjD"
                     /locus_tag="OO335_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6002723.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23835..24734)
                     /locus_tag="OO335_002364"
     CDS             complement(23835..24734)
                     /locus_tag="OO335_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6002724.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            25254..>25855
                     /locus_tag="OO335_002365"
     CDS             25254..>25855
                     /locus_tag="OO335_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6002725.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6158 a         6499 c         7035 g         6163 t
ORIGIN      
        1 ggaagtaacg tgtcatgcgg gcgtcaggct gccgtaatgg caatttgcgc ccggatcagg
       61 ccgcagaggt taaactctgc ggcctttttc gtgcttactg cgggtaaggc acccagtcgc
      121 cgccgttcag gcgaacgtac ggtttatctt ggtattgaat aactactgca tttgagttct
      181 cggagaccgg tgctgtttgt ggcaacccac tggtgagttt tttccagtca acattgtctt
      241 cggtgaaaat cttgccatca agaacgcgaa ccaccagatc ggagatagcc aggaagctgc
      301 tcggttggtc aatgacaatc ggtgccccct gatgtggtgc cttcatgccg aagaatttca
      361 ccccaacggg gacgtctgtg atagacgggc tagggatatc acgtaggcca gatacctgca
      421 ttctgtcgcc cttcagcgcg ccgccgtgtt ccggcaccac gaccaccatc actttacgac
      481 ccgatttctc cagttcagta aagaaggcgt ccagttcatc aaagaatttc tgcgcccgcg
      541 ctttgtaatc cgctgttttg ctgacccccg gataatggtt gccgtcatgc agtggaagcg
      601 tgttgtagaa cgtggcacta cggctgtttt tatctttttc ggtaacgtcc agccagcggt
      661 taagcacggc ggtatcgtca taaaccggcg aaccatcaaa gcccagcaaa ataaccggca
      721 gatttgtttg atccatcaat tcagtctgca tgccgccatt ttcgcgaact tctttcaaaa
      781 aaccgccgaa ctggccgtta tgtcccatca tcaggtgctg ggtaaagccc agtttcgaaa
      841 ggttatcaaa cagatagcag tcgttatttg ccggttgata cagattagtg tgcgaagtct
      901 gcccgcagct ggcgcgcagt aaacggatcg ccgccgggcc actgtaggag gtggcggagt
      961 taaagttctt gaaatcaata tcgaaatgcg accacagtgg atgcgacatc aacccggcgg
     1021 cttctatatc cgaccaggaa agcgaacaga tgttaatcac cagtagttca aatggctgag
     1081 catcagcggg cagcgaagac gggaaggtcg atttacgttt cgcctccgcg ttatagaaat
     1141 tattcagcca ggcgttaagg ttcgccgttg ttggcggtgc agtttgtgcg ggcatatcac
     1201 ccactaccgg tgcgccaccc gtcgccgcaa cggttgccgc tgcgttacca cccgtcgttg
     1261 ttacagtggt cgtcggttgt ccggctggcc acaaggagaa acttggtccc gccagggtaa
     1321 gtacgttcag ccatagcagt atggcaacca caaaaacggt aatgcgaatc cattgtgaca
     1381 ggaataacca ggccactaat aaaacaaaaa tggccccaat catctgccag ttaataaagc
     1441 gtgtgacaag gtcgattaaa taatcggtac tgaaccccgc cacctgcgaa ccctggctca
     1501 ttatgctttc cgggccaggc aaccagatgt catgccagaa caaagcaaag ccgatcggca
     1561 gggcaatcca gtggcgcaag cgatgcaggc tgtaacgcgg aatgggcatc agcagaaacg
     1621 cggcaaacac caaattgagg agcgggtgga agttaagata tcccgcccac aacaggccaa
     1681 acttaaccag aaaatagaag ttccagccgg aaaggccgcg ccagtattgc cagagggaag
     1741 aaggcatggc ggtattttgc gtaaattgag tcattttttg gttgccctgg ctttttccgt
     1801 gcggcgtaac gtcccggccg gtttaacata acgaggttta gcaaagaaca aacgtaaggt
     1861 gtcgcgaatg cgtcgcaaag agtgccgcgc cagatagccc agcgggaaaa atatcagtgc
     1921 gcaaacgata ataatttcaa tgatatcgct gatggtcatc atgatgagcg ctccacagca
     1981 tcatctaaca gtcgcatggg ttctggtatt cgtcgccagt ggcgaccatc gtgctcggca
     2041 ttgatgaccg gtttagaact ttgcgttaaa ggcagcggca ttccccattg ttctggggca
     2101 agcaggcgca tctgcaccag ctcggcactg atttgatcat cttcaaacca gaccatacgg
     2161 tttgagaaaa tgtcgccagt aggcaatggg aaaatatgat tcaacgcggt atccagatcg
     2221 ttaatccgac agaatgagag aaacagcacc agccgattac cgccaatggt catgatatcg
     2281 ccggtacggt tagggcgaca tagcgtcagg gcttgctcaa cgcggatacc cggtaccgga
     2341 cgtagggcaa ccagaacgcc tttaccgtgg gcaggcaaga gagggttatt catcatgttg
     2401 ttgacggcat tacagaacac atcccacttc tggaaaccac gcagtttgag cggctgggtc
     2461 attgacagca aggtagtgat atcttccggt acatagcgac taaacttctg cccttgcacg
     2521 ctttcgatca tcgtcagaca acgggagagt ggcgcattcc acggaataac catatttgca
     2581 ccgcaggcca ataacaaacg ttcatcggtg gcgcgcaggc tagcggtatt ttcccgcacg
     2641 aggattttca tcgcactacc gcgctggcga cgcagggtat gaatgctgcg ggccagtggc
     2701 tcgatttgcg cattttgctg taaagaaaag accaccgtcg ccgcctgagc ggtacgggct
     2761 tcattgaaca ggacttcgtt attgttgaac agttgccagt gttccgatag cggcggcgca
     2821 ccttccagta cagcaacatt actcagaatg cgtttttcgt cgctgcgtgg ttggatctcc
     2881 gcctcttcgc tttgaactaa tgtccagata ccattttgtt gctgaacgct aagctgctga
     2941 cgggcgctga cccctttttc gttgcaccag aaggcaatat ccagcaaatg ttggtcaccc
     3001 tgaaaacgca aactggcaag accaaaaagt gaacggtatt cctcaagcaa caatgaaaat
     3061 tgtttatcgt tattatttcc gggattaatt accaaaagcg aacaatggtt taacctgctc
     3121 catttattca ttttatccaa ccatgagcga agccgctcgg caggaatatt ttgccatgcg
     3181 ttatttgcgc aaacaagaat aaaaaggtaa ttatggggat caatagaaca ctgcaaatca
     3241 cgggccaaat agtatagacc tttttcatga tttagcattg aaaataattt tattttttcc
     3301 ggcccttgag aatcatctaa ttgaaagatt ttcgccggat cgctgtccat gctaatgacc
     3361 gcgacgtgtg cggtttcagc ctgggatgca attgtttgat tcgccagact gatagcatct
     3421 tcatggcgat cgacgttaaa ccaccagacg ccgcctgctg gcatatgtcg cagctcatcc
     3481 cataatgatg agataccgat agagaataca gggtccacaa tgtccctcat gtcaccattt
     3541 tgtctgatta tcagtttact agcgaaagct aagaaataaa cctaccattg aaattaaaac
     3601 ttatcagatt tagcatgtga atcaatttag aaggtcacaa gacgtgtgat tttagtcttt
     3661 tcgtttttaa ctttttctgt tgcgtaacgg aaagtcaaaa agtgagcaaa ttcccgtctc
     3721 gccgtaaaca ataaaccggc gaaatgctca cggttagaat tgtctcatga atggtacggt
     3781 tatttcatag ggatcaagca aaatgaataa caatgaacca gatactctgc ctgatcccgc
     3841 gataggctat atcttccaga atgatattgt ggcgttaaag caggcatttt cactgcctga
     3901 tattgattat gccgatattt cccaacgcga acagttggcc gcggcattaa aacgctggcc
     3961 gttgctggca gagtttgcgc aacaaaagta ggggattggt gaatggccgt actgggattg
     4021 cagggggtgc ggggaggcgt ggggacaaca accatcaccg ccgcattagc ctggtcatta
     4081 caaatgttgg gagaaaatgt cctggtggtc gatgcctgcc cggacaactt gttgcgcctg
     4141 tcatttaatg ttgattttac ccaccgtcag ggctgggcca gagcgatgct ggatggccag
     4201 gactggcgtg acgctgggtt gcgctacacc tcgcagctcg atttgctgcc ttttggtcag
     4261 ttatccattg aagaacaaga aaatccacag cactggcaaa cccggctgag cgatatttgc
     4321 tccggcttac agcaactaaa agccagcggg cgttaccagt ggattttaat cgacttaccg
     4381 cgtgatgcct cgcagataac ccaccagctg ctgagtttgt gcgagcactc gctggcaatc
     4441 gtcaatgtgg atgccaactg ccatatccga ctgcatcagc aagcgctgcc ggatggcgca
     4501 catattttga ttaatgactt ccgtattggc agtcaggttc aggacgatat ttaccagctt
     4561 tggttgcaaa gccagcgccg attactgccg atgctcattc atcgtgatga agcgatggct
     4621 gaatgcctgg cggctaagca accagtaggt gaatatcgca gtgatgcgct ggcggctgaa
     4681 gagatactga cgctggcgaa ctggtgcctg ttgaactact ccgggctgaa aacgccagtc
     4741 gggagtgcat catgagtatc ctgacccggt ggttgcttat cccgccggtc aacgcgcggc
     4801 ttatcgggcg ttatcgcgat tatcgtcgtc acggtgcgtc ggctttcagc gcgacgctcg
     4861 gctgtttctg gatgatcctg gcctggattt ttattccgct ggagcacccg cgctggcagc
     4921 gtattcgcgc agaacataaa aacctgtata cgcatatcaa cgcctcgcgt ccgcgtccgc
     4981 tggacccggt ccgttatctc attcaaacat gctggttatt gatcggtgca tcgcgcaaag
     5041 aaacgccgaa accgcgcagc gggcattttc aggtctgcaa aatattcgtg gacgttacca
     5101 tcaatggatg aacgagctgc ctgagcgcgt tagccataaa acacagcatc tggatgaaaa
     5161 aaaagagctc ggtcatttga gtgccggggc gcggcggttg atcctcggta tcatcgtcac
     5221 cttctcgctg attctggcgt taatctgcgt tactcagccg tttaacccgc tggcgcagtt
     5281 tatcttcctg atgctgctgt ggggggtagc gctgatcgta cggcggatgc cggggcgctt
     5341 ctcggcgcta atgttgattg tgctgtcgct gaccgtttct tgccgttata tctggtggcg
     5401 ttacacctct acgctgaact gggacgatcc ggtcagcctg gtgtgcgggc ttattctgct
     5461 cttcgctgaa acgtacgcgt ggattgtgct ggtgctcggc tacttccagg tagtatggcc
     5521 gctgaatcgt cagccggtgc cattgccgaa ggatatgtcg ctgtggccgt cagtggatat
     5581 ctttgtcccg acttacaacg aagatctcaa tgtggtgaaa aataccattt acgcctcgct
     5641 gggtatcgac tggccgaaag acaaactgaa catctggatc ctcgatgacg gcggcaggga
     5701 agagtttcgc cagtttgcgc aaaacgtggg ggtgaaatat atcgcccgca ccactcatga
     5761 acatgcgaaa gcgggcaaca tcaacaatgc gctgaaatat gccaaaggcg agttcgtgtc
     5821 gattttcgac tgcgaccacg taccaacgcg atcgttcctg caaatgacca tgggctggtt
     5881 cctgaaagag aaacagctgg cgatgatgca gactccacac cacttcttct cgccggaccc
     5941 gtttgaacgc aacctgggac gttttcgtaa aacgccgaac gaaggcacgc tgttctatgg
     6001 tctggtgcag gatggcaacg atatgtggga cgccactttc ttctgcggtt cctgtgcggt
     6061 gattcgtcgt aagccgctgg atgaaattgg cggcattgct gtcgaaaccg tgactgaaga
     6121 tgcgcatact tctctgcgtt tgcaccgtcg tggctatacc tccgcgtata tgcgtattcc
     6181 gcaggcggcg gggctggcga ccgaaagtct gtcggcgcat atcggtcagc gtattcgctg
     6241 ggcgcgcggg atggtataaa tcttccgtct cgataacccg ctcaccggta aaggactgaa
     6301 gtttgctcag cggctgtgct acgtcaacgc catgttccac ttcttgtcgg gcattccacg
     6361 gctgatcttc ctgactgcgc cgctggcgtt cctgctgctt catgcctaca tcatctatgc
     6421 gccagcgttg atgatcgccc tattcgtgct gccgcatatg atccatgcca gcctgaccaa
     6481 ctcgaagatc cagggcaaat atcgccactc tttctggagt gaaatctacg aaacggtgct
     6541 ggcgtggtat atcgcaccac cgacgctggt ggcgctgatt aacccgcaca aaggcaaatt
     6601 taacgtcacc gccaaaggtg gactggtgga agaagagtac gtcgactggg tgatctcgcg
     6661 gccctacatc ttccttgttc tactcaacct ggtgggcgtt gcggtaggca tctggcgcta
     6721 cttctatggc ccgccaaccg agatgctcac cgtggtcgtc agtatggtgt gggtgttcta
     6781 caacctgatt gttcttggcg gcgcagttgc ggtatcggta gaaagcaaac aggtacgccg
     6841 atcgaaccgc gtggagatga cgatgcccgc ggcaattgcc cgcgaagatg gtcacctctt
     6901 ctcgtgtacc gttcaggatt tctccgacgg tggtttgggg atcaagatca acggtcaggc
     6961 gcagattctg gaagggcaga aagtgaatct gttgcttaaa cgcggtcagc aggaatacgt
     7021 cttcccgacc caggtggcgc gcgtgatggg taatgaagtt gggctgaaat taatgccgct
     7081 caccacccag caacatatcg attttgtgca gtgtacgttt gcccgtgcgg atacatgggc
     7141 gctctggcag gacagctacc cggaagataa gccgctggaa agtctgctgg atattctgaa
     7201 gctcggcttc cgtggctacc gccatctggc ggagtttgcg ccttcttcgg tgaagggcat
     7261 attccgtgtg ctgacttctc tggtttcctg ggttgtatcg tttattccgc gccgcccgga
     7321 gcggagcgaa acggcacaac catcggatca ggctttggct caacaatgat gataacgcga
     7381 tgaaaagaaa actattctgg atttgtgcag tggctatggg gatgagtgcg ttcccctctt
     7441 tcatgacgca ggcgacgcca gcaacgcaac cactgatcaa tgctgagcca gctgtagccg
     7501 cccagacgga acaaaatccg caggtggggc aagtgatgtc aggcgagcag ggagctgatg
     7561 cccccatcgt ggcgcagaac ggtccttcgc gtgatgtgaa gctgaccttt gcgcaaatcg
     7621 cgccgccgcc gggcagcatg gtgctacgtg gtattaaccc gaacggcagc attgagtttg
     7681 gtatgcgcag cgatgaagtg gtgacgaagg cgatgctcaa cttcgaatac acgccatcgc
     7741 catcgttact gcctgtccag tcgcagttaa aggtttatct caatgatgag ctgatgggcg
     7801 tgctgccagt gaccaaagaa cagttgggta aaaaaacgct ggcgcaaatg cccattaatc
     7861 cactgtttat taccgacttc aaccgtgtgc ggctggagtt tgtcggccat tatcaggacg
     7921 tgtgcgaaaa cccggccagc accacgcttt ggctggatgt tgggcggagc agtggactgg
     7981 atctgaccta tcagaccctg aatgtgaaga atgacctgtc acacttcccg gtgccattct
     8041 ttgacccgcg cgataaccgt actaacacct tgccgatggt ctttgcgggt gcgccggatg
     8101 ttgggctgca acaagcatct gccattgtcg cctcgtggtt tggttcgcgt tctggctggc
     8161 gtgggcagaa cttcccggtg ctctataacc aattgccgga tcgcaatgcc attgtctttg
     8221 caaccaacga caaacggccg gacttcctgc gcgatcatcc ggcggtaaaa gccccggtga
     8281 ttgagatgat taaccatccg cagaatcctt acgtcaaact gctggtggtg tttggtcgtg
     8341 acgacaaaga cctgttgcag gcagcgaaag gtatcgctca gggtaacatt ctgttccgtg
     8401 gtgaaagcgt ggtagtgaat gaagtgaaac cgctgctacc gcgtaagccg tacgatgcgc
     8461 cgaactgggt acgtaccgat cgtccggtca catttggcga actgaaaacc tatgaagaac
     8521 agttacaatc cagcggcctt gagccagcag cgattaacgt ttcgctaaac ctgccgccgg
     8581 atctctacct gatgcgcagt accggcattg atatggatat taattaccgc tacaccatgc
     8641 cgccggtgaa agacagttcg cggatggata tcagcctgaa taaccagttc ctgcaatcct
     8701 tcaacctgag cagcaaacag gaggcgaacc gtctgctgct gcgtattccg gtattacaag
     8761 gtttgctgga tggcaaaaca gatgtctcta ttccggcgct gaaactgggc gcgaccaacc
     8821 agctgcgctt cgactttgag tatatgaacc cgatgccagg cggttcggta gataactgta
     8881 ttaccttcca gccggtgcag aatcatgtgg tgattggtga cgattccacc atcgacttct
     8941 cgaagtatta ccacttcatc ccgatgccgg atctacgcgc cttatcccga tgccggatct
     9001 acgcgccttt gctaacgcgg gcttcccatt cagccggatg gcggatctgt cgcaaaccat
     9061 caccgtgatg ccgaaagcgc ctaacgaagc acagatggaa acgttgctga atactgttgg
     9121 ttttatcggc gcacagacgg gcttcccggc gattaatctg acggtgaccg atgatggcag
     9181 caccattcag ggcaaagatg ccgacatcat gatcatcggt ggtatcccgg acaaactgaa
     9241 agacgataag cagatcgacc tattggtgca ggcgaccgaa agctgggtga aaacaccgat
     9301 gcgccagacc ccgttccccg gcattgtgcc ggacgagagc gatcgcgcgg cagaaaccca
     9361 gtcaacgctg acctcttccg gtgcgatggc ggcggtgatt ggcttccagt cgccgtataa
     9421 cgaccagcgc agcgtgattg cgctgttggc agatagccca cgcggttatg aaatgcttaa
     9481 cgatgcggtg aacgatagcg gcaaacgcgc caccatgttc ggttcggtcg cggtgatccg
     9541 cgagtccggt atcaacagcc tacgtgttgg cgacgtttat tacgtaggtc atctgccgtg
     9601 gttcgagcgc ttgtggtatg cgctggcaaa ccatccgatt ctgctggcgg tgctggcgac
     9661 tatcagtgtg atattgctgg catgggtact gtggcgtctg ctgcgaatta ttagtcgtcg
     9721 tcgtcttaac ccggataacg agtaattgaa gatgaatgtg ttgcgtagtg gaatcgtgac
     9781 gatgctgttg ttggctgcct ttagtgttca ggcagcctgt acctggcctg cctgggagca
     9841 gtttaaaaag gattacatca gtcaggaagg gcgcgtcatc gaccccagcg acgcgcgcaa
     9901 aatcaccacc tccgaagggc aaagttacgg tatgttcttt gccctggcgg ctaacgaccg
     9961 tgcagctttc gataatattc tcgactggac gcagaacaat ctcgctcagg gttctttaaa
    10021 agaacgtttg cccgcctggc tgtggggcaa gaaagagaac agtaagtggg aagtgctgga
    10081 cagcaattcg gcctccgatg gtgatgtctg gatggcctgg tcgttgctgg aggcggggcg
    10141 tttgtggaaa gagcagcgtt ataccgacat cggcagcgcg ttgctaaaac gtatcgcgcg
    10201 ggaggaagtg gtgacggtgc ctgggctggg ttccatgttg ttaccgggca aagtgggttt
    10261 tgctgaggat aacagctggc gttttaaccc cagctacatg ccgccgacgc tggcgcagta
    10321 tttcacccgc tttggcgcgc cgtggaccac gctgcgcgaa accaatcaac atttattgct
    10381 ggaaaccgcc ccgaaaggct tttcgccaga ctgggtgcgc tatgagaaag acaaaggctg
    10441 gcagctaaaa gccgaaaaaa cattgatcag cagctacgac gctatccgcg tttacatgtg
    10501 ggtaggcatg atgcctgaca gcgatccgca aaaagcgcgg atgctcaacc ggtttaaacc
    10561 gatggcgaca ttcactgaga aaaacggtta tccgccggaa aaagtggatg tggctacggg
    10621 gaaagcgcag ggtaaaggac cggtcggttt ttctgccgcc atgctgcctt ttttacaaaa
    10681 ccgcgatgcg caggcagttc agcgccagcg cgtggccgat aactttcccg gcagcgatac
    10741 ctattacaac tatgtgctga ccctgtttgg acaaggctgg gatcaacacc gtttccgctt
    10801 ctcgacaaaa ggtgagttat tacctgactg gggccaggaa tgcgcaaatt cacactaaac
    10861 atattcacgc tttccctcgg tctggccgtc atgccgatgg tcgaggcagc accaaccatc
    10921 agcagaccct gcggatggac agcggcaaca ctaatgccgt gcgcgggctg gcgaatattt
    10981 accgccagca gtcgccagaa aaagctgaag cgtttatcgc ctcgctctct gccagccagc
    11041 ggcgcagcat tgatgatatc gaacgcagcc tgcaaaacga ccgtctggca cagcaggcag
    11101 aggtactgga aaaccagggc aaatgggcgc aggcggcagc acttcagcgg caacgactgg
    11161 cgctgggccc cggcagcgta tggattactt accgactggc aggaagaagc tgcgcggcaa
    11221 tccaacgatg gcggcagcag tcagggcttc ggctacacgg cgcgggcatt acttgaacga
    11281 cgtgttactt ccaactggtt tgttggcacg gcaattgata tccagcaggt gaaagattac
    11341 gcacccagcc atttcctgct ctacgtacgt tattccgccg ccggatggca gggtgacatg
    11401 gatttaccgc cgcagccgct gataccttac gccgactggt aagttttcag atagcgcctc
    11461 tcttaatgcc gctgcgatcg ggtatactcg ggcggcaatc tgggatttcc ggggggagac
    11521 aatttgcgcg taagtcgctc gttaacaatc aagcagatgg caatggtggc agccgttgtc
    11581 ctggtgttcg tttttatttt ttgcaccgtt ttgctgttcc atctggtcca gcagaatcgc
    11641 tataacacgg ctacgcaact ggaaagcatt gctcgctctg tccgcgaacc cttatcttca
    11701 gctattttga aaggcgatat tcccgaagcg gaagctattc ttgccagcat taaaccggca
    11761 ggcgtggtca gccgtgccga tgtagtgctg cctaaccagt tccaggcgct gcgtaaaagt
    11821 tttattccag agcgtccggt gccggtaatg gttactcgcc tgtttgagct accggttcaa
    11881 atctcgctgg gcgtctactc gctcgaacgt ccggcaaatc cgcagccaat agcctatctg
    11941 gtgctacagg cggattcctt ccgtatgtat aagttcgtga tgagcactct ctcaacgtta
    12001 gtgaccattt acttactttt gtcgcttatc ctgaccgtcg ccatcagctg gtgcattaac
    12061 cgcctgattt tgcatccgtt acgcaatatt gctcgcgaac ttaacgccat cccagcccag
    12121 gagcttgttg gtcaccaact ggcattaccg cgtctgcatc aggacgatga aatcggtatg
    12181 ttggtgcgca gttacaacct caaccagcaa ttgctgcagc gccattatga agaacagaac
    12241 gaaaatgcga tgcgcttccc ggtgtcggat ttgccgaaca aagccttgct gatggagatg
    12301 ctggagcagg ttgtcgcgcg taaacaaacc accgcgctga tgatcatcac ctgtgaaacc
    12361 ctgcgtgata ctgcgggcgt gctgaaagag gcgcaacgag aaattctgct gctgacgctg
    12421 gtggaaaaac tcaaatcggt actgtcgcca cgtatgatcc tcgcgcagat tagcggttat
    12481 gactttgctg tcattgccaa cggtgtacag gaaccgtggc acgcaatcac tttgggtcag
    12541 caagtactca ctatcatgag cgagcgcctg ccgattgaac gtattcaact ccgtccgcac
    12601 tgtagcattg gcgtggcgat gttctacggc gatctcaccg ccgaacagct ttacagtcgc
    12661 gctatttctg cggcatttac cgctcgccat aaaggcaaga atcagattca gttctttgat
    12721 ccgcagcaga tggaagcagc ccagaagcgg ttgacggaag agagcgatat ccttaatgca
    12781 ctggaaaatc atcagtttgc aatttggtta cagccacagg tcgagatgac cagcggtaaa
    12841 ctggtcagtg cggaagtgtt actgcgtatc cagcaaccgg atggcagttg ggacctgccg
    12901 gatggcttaa tcgatcgcat tgagtgctgt gggctgatgg ttaccgtcgg tcactgggtg
    12961 ctggaagagt cctgtcgatt gcttgcagcc tggcaagagc gcggcattat gctgcccttg
    13021 tcggtaaacc tctctgcgct gcaactgatg cacccgaata tggtggcgga tatgctggaa
    13081 ctgttaaccc gctatcgcat tcagccggga acactgattc tggaagtgac agaaagccga
    13141 cgtattgacg accctcatgc tgcggtggca atcctccgtc cgctgcgcaa tgccggagtt
    13201 cgggtggcgc tggatgattt cggcatgggc tacgcagggc tgcgtcagct gcagcatatg
    13261 aaatcgttgc caatcgacgt actgaaaatc gacaaaatgt ttgttgaagg cttgccggaa
    13321 gatagcagca tgattgctgc aattatcatg ctggcgcaga gcctgaactt acaaatgatt
    13381 gccgaaggcg tggagactga agcacaacga gactggctgg caaaagcggg cgttggtatt
    13441 gcccagggct tcctttttgc tcgcccactc cctattgaaa tcttcgaaga gagttacctg
    13501 gaagaaaagt agctacccca aactgattac aaaactttaa aaagtgctgg tttgtgcgag
    13561 ccagctcaaa ctttttaacc tttttgtttc aattatgatc caggtacatt tctgtgatgt
    13621 tgtctgggtg ttattttaag gccgcaggta cccccccccc ataaccttac aagacctgtg
    13681 gttttactaa aggacaccct atgaaaacct ctctgtttaa aagcctttac tttcaggtcc
    13741 tgacagcgat agccattggt attctccttg gccatttcta tcctgaaata ggcgagcaaa
    13801 tgaaaccgct tggcgacggc ttcgttaagc tcattaagat gatcatcgct cctgtcatct
    13861 tttgtaccgt cgtaacgggc attgcgggca tggaaagcat gaaggcggtc ggtcgtaccg
    13921 gcgcagtcgc actgctttac tttgaaattg tcagtaccat cgcgctgatt attggtctta
    13981 tcatcgttaa cgtcgtgcag cctggtgccg gaatgaacgt cgatccggca acgcttgatg
    14041 cgaaagcggt agcggtttac gccgatcagg cgaaagacca gggcattgtc gccttcatta
    14101 tggatgtcat cccggcgagc gtcattggcg catttgccag cggtaacatt ctgcaggtgc
    14161 tgctgtttgc cgtactgttt ggttttgcgc tccaccgtct gggcagcaaa ggccaactga
    14221 tttttaacgt gatcgaaagt ttctcgcagg tcatcttcgg catcatcaat atgatcatgc
    14281 gtctggcacc tattggtgcg ctcggggcaa tggcgtttac catcggtaaa tacggcgtcg
    14341 gcacactggt gcaactgggg cagctgatta tctgtttcta cattacctgt atcctgtttg
    14401 tggtgctggt attgggttca atcgctaaag cgactggttt cagtatcttc aaatttatcc
    14461 gctacatccg tgaagaactg ctgattgtac tggggacttc atcttccgag tcggcgctgc
    14521 cgcgtatgct cgacaagatg gagaaactcg gctgccgtaa atcggtggtg gggctggtca
    14581 tcccgacagg ctactcgttt aaccttgatg gcacatcgat atacctgaca atggcggcgg
    14641 tgtttatcgc ccaggccact aacagtcaga tggatatcgt ccaccaaatc acgctgttaa
    14701 tcgtgttgct gctttcttct aaaggggcgg caggggtaac gggtagtggc tttatcgtgc
    14761 tggcggcgac gctctctgcg gtgggccatt tgccggtagc gggtctggcg ctgatcctcg
    14821 gtatcgaccg ctttatgtca gaagctcgtg cgctaactaa cctggtcggt aacggcgtag
    14881 cgaccattgt cgttgctaag tgggtgaaag aactggacca caaaaaactg gacgatgtgc
    14941 tgaataatcg tgcgccggat ggcaaaacgc acgaattatc ctcttaatct caccacttat
    15001 gcccgcactc ccttccccga gtgcgggtgt ttgcgcataa ttgacccctg tgcctgaact
    15061 taccacctgg gtgagttcag gtttatttca cttcttccgt gacattttca tgttcttgcg
    15121 gtctaacacg aagtgttttt aacgtcattt agactgaccg acaacgggca gtttttttat
    15181 taccaggata gttgatcagg ggttcacatg cagggcataa aaattcgact tttagcgggc
    15241 ggtttgctga tgatggccac tgctggctat gtgcaggcag atgcgctcca gcctgatcca
    15301 gcatggcaac aggggacgct ttccaacggt ttacagtggc aagtgctgac caccccccag
    15361 cgtcccagcg atcgtgttga aattcgcctg ctggttaata ccggttcgct cgccgaaagt
    15421 acacaacaga gcggttacag tcacgccatc cctcgtattg cgctaacgca aagcggtggc
    15481 cttgacgcag cacaggcgcg ttcattgtgg cagcagggga tcgaccctaa acgcccgatg
    15541 ccgccggtaa ttgtctctta tgacaccacg ctgtttaatc tgagtttgcc caataaccgt
    15601 aacgatttgc tgaaagaagc gctctcttat ctggcaaatg ccactggcaa attgaccatc
    15661 acaccagaaa ccatcaacca cgcgctgcaa agtcaggaca tggtggcaac ctggcctgcc
    15721 gatactaaag agggctggtg gcgctatcgt ctgaaagggt caaccttgtt aggtcacgat
    15781 cctgccgatc cgctgaaaca acccgttgaa gcggaaaaaa ttaaagattt ctatcagaaa
    15841 tggtacaccc cggatgcaat gacgctactg gtggtgggaa acgtggatgc gcgctcggtt
    15901 gtcgaccaaa tcaacaaaac gtttggcgaa ctgaaaggca aacgtgaaac gccagctccg
    15961 gtgccgacgc tttctccgct gcgtgcggaa gcggtgagta ttatgactga cgcggtgcgt
    16021 caggaccggt tatctatcat gtgggatacg ccgtggcagc cgattcgtga atcagccgca
    16081 ctgctgcgct actggcgtgc ggacctggcc cgcgaggcgc tgttctggca tgttcagcaa
    16141 gcgttaagcg ccagtgacag caaagacatc ggtcttggat ttgactgccg tgtgctgtat
    16201 ctgcgtgcgc agtgtgccat caacatcgaa tcaccaaacg acaagctgaa cagcaacctt
    16261 aatctggtgg cgcgtgaact ggcgaaggtt cgcgataaag gtctgccgga agaagagttc
    16321 aatgcgttag tggcgcaaaa gaaactggag ctgcagaaac tgtttgccgc ctatgcacgg
    16381 gctgataccg atattctgat gggccagcgg atgcgttcgt tgcaaaatca ggtcgtcgat
    16441 atcgcgccgg agcagtatca gaaactgcgg caggatttcc ttaacagcct gactgtggag
    16501 atgttaaatc aggatctgcg tcagcagttg tcgaatgata tggcgttaat actgctgcag
    16561 ccgaaaggcg agccggaatt taacatgaaa gcgttgcagg cggcctggga tcaaatcatg
    16621 gccccatcta ccgccgctgc gaccacctct gtcgccacgg atgacgtaca tcctgaagtg
    16681 acggatattc cacctgcaca gtaatgcctg atggtgatga gttagcacaa ccatcaggta
    16741 tttatggatg agctggatag aggggtaaca ctttatccag ccttttgcat atgctgcgtt
    16801 tacgctggca tcgcctcacg cgggataatc gcgccgcgat actgaataac ggtacttgcg
    16861 gtcaggtgcc cgcgtttcgc cgcgttttcc gcactgccgc ccgtcaaacg taccgccaga
    16921 taaccggcgc tgaaagagtc gccagctgcg gtggtatcga tcactttttc tttcggcagt
    16981 ttcaccgccg gaacatcaac taacgcttca ccagcaatgg acaccaggca agaatccgcc
    17041 ccgcgtttca ccaccacttc tttcacgccc gcgttatggg tgcgcgcaat gacgtcttcc
    17101 accggctgtt gaccccacag cgcgtcttcg tcgtccagcg tcaggaaggc gatatccgtg
    17161 cattccagca tttgctggta cacctgctgt gtctcttctt tgctggccca caggcgcgga
    17221 cgatagttat tgtcgaaaat cacttttccg cctttggcgc ggcattcgcg cagcagggaa
    17281 agcagctttt cgcggctggt cgggcttaag atcgccaggc taatcccgct caggtagaga
    17341 taatcgaaat tcgccagctc ttcgcaaatc gccgcagact gctcgctcgc cagccagaat
    17401 ttggcggcgg cttcgttacg ccagtagtag aacgtacgtt cgccggtgct gtcggtttca
    17461 atgtagtaaa ggcccggaag acggttttcc atccgttggg tcagggaagt atcaacgttc
    17521 tcgccgtgcc aggcgtccag catctgctgg ctaaaactgt ccgttcccag cgccgttacg
    17581 taatgaacgg ttaatgccgc aggatcgacc tgacgggcga tatagacgga agtgttcagg
    17641 gtatcgccgc cgaaaccgcg cttaacgtcc gcgcctttct cggaaagctc aatcatgcat
    17701 tcgccaatca cggcaatctt tttggacata gtcgtgaacc tgatctgtaa aaaattagcg
    17761 ttagtttgcg ctgtggtggt ttgctggtca accatattaa aacagtgttc cattattttt
    17821 ttgagccaga acgtattcct gaaagattcc gttgtggagt gaggaaattt accccggcca
    17881 ggcggtaaag ttctgccctt acgcgccgat aatctttgtc gagtccgggc agcatcactt
    17941 ttaaacacag gacatctttg atgataaggc aggttatcca gcgaataagc aaccctgaag
    18001 caagcatcga gagcttgcag gaacggcgtt tttggttgca gtgtgagcgt gcttacacct
    18061 ggcagccgat ctatcaaaca tgcgggcggt taatggccgt ggagctatta acggtggtca
    18121 cgcatccctt gaacccttcg caacgcctgc cgccggatcg ctattttact gaaatcaccg
    18181 tcagccatcg gatggaggtt gtgaaagagc agattgattt gctggcgcaa aaagccgact
    18241 tctttgtaga gcacggcctg ctggcatcgg tcaatattga tggccctacg ctcatcgccc
    18301 tgcgtcagca accaaaaatc ctgcgccaga ttgagcgtct tccctggctg cgtttcgaac
    18361 tggtggagca tatccgtctg ccgaaagatt caacctttgc ctcgatgtgt gaatttggcc
    18421 cgctgtggct ggatgatttt ggtaccggga tggcaaattt ctctgcacta agtgaagtgc
    18481 gttatgacta catcaaaatc gcgcgagaac tgtttgtgat gctgcgtcag tcgccggaag
    18541 gccgcacact cttttctcag cttttacatc taatgaatcg ctattgtcgc ggggtgattg
    18601 tcgagggcgt agaaacaccg gaagagtggc gtgatgttca gaactcgccc gcattcgccg
    18661 cacaaggctg gtttctttca cgcccggcac cgatggaaac gctgaatacg gcggttctgg
    18721 cgctataagc tgcctcattt tccgccgggc tggactatct ttaggactgg cacaggaaga
    18781 atatgaggaa gcgaacgatg agcaaggcag gcaaaataac cgctgcgatt tcaggggctt
    18841 tcttgttgtt gattgtcgtg gcgatcattt tgattgcaac ttttgactgg aatcgactca
    18901 aaccgaccat caaccagaaa gtctctgcgg agttgaatcg tccgttcgct atccgtggca
    18961 atctgggcgt ggtgtgggag cggcaaaaac aagaaactgg ctggcgcagc tgggtgccgt
    19021 ggcctcatgt acatgcagaa gacatcattc ttggtaatcc accggatatt cccgaagtca
    19081 cgatggtgca tttgccgcga gtagaggcaa cgctggcacc gctggcgctg ctgaccaaaa
    19141 cggtctggct gccgtggatc aagctcgaaa agcccgacgc gcgcctgatt cgcctctctg
    19201 aaaagaacaa taactggacg tttaatcttg ccaacgatga caacaaagac gcgaatgcaa
    19261 agccgtcggc atggtcgttt cggctggata atattctttt cgatcaaggg cggatcgcca
    19321 ttgatgacaa agtaagcaaa gcggatctgg aaatttttgt tgatccgtta ggcaagccgc
    19381 tgccattcag cgaagttact ggatcgaaag gtaaagcgga taaagaaaag gtgggcgatt
    19441 acatttttgg cctgaaggcg cagggacgtt ataacggtga accgctcacg ggtacgggaa
    19501 aaataggcgg tatgctggcg ctgcgtggcg aagggacgcc gtttccggta caggctgatt
    19561 tccgttcagg taatacccgt gttgcttttg atggcgtcgt gaatgaccca atgaagatgg
    19621 gcggtgtcga tttacggctt aaattttctg gcgattcact gggtgatctc tatgaactga
    19681 cgggcgttct gctgcccgat accccgccgt ttgaaacgga tggtcggctg gtagcgaaaa
    19741 tcgacactga aaaatcgtcg gtctttgatt atcgcggttt taatgggcga attggcgata
    19801 gcgatatcca cggttctctg gtctacacca ccggaaagcc acgaccaaaa ctggaaggtg
    19861 atgtcgagtc gcggcaattg cggctggcgg acctgggacc gttgattggc gttgattccg
    19921 ggaaaggggc agaaaagtcg aaacggtctg aacagaagaa gggcgaaaaa agcgttcagc
    19981 ctgcgggcaa agtgctgcct tatgaccgct tcgaaaccga taaatgggac gttatggatg
    20041 ccgatgttcg cttcaaaggg cggcgcattg agcatggcag tagcctgccg attagcgatc
    20101 tttctactca tatcatcctc aaaaatgctg acctgcgcct gcaaccgctg aaatttggca
    20161 tggcgggcgg cagcattgcg gcgaatattc atctggaagg cgataaaaag ccgatgcagg
    20221 ggcgggcaga tattcaggct cgtcgactga aactgaaaga actgatgccc gatgtggaac
    20281 tgatgcagaa gacgctgggg gaaatgaacg gtgacgcgga actacgcggt agcggtaact
    20341 cggtggcggc gcttttaggc aacagtaacg gcaacctgaa actgttgatg aatgacgggc
    20401 tggtgagccg caacctgatg gagattgttg ggctgaatgt cggcaactac attgtcggtg
    20461 cgatatttgg tgacgatgag gtgcgggtga actgcgcggc ggcgaatctg aatattgcca
    20521 acggcgtggc gcgcccgcag atttttgctt tcgatactga gaacgcgttg attaatgtta
    20581 ccggcacggc aagttttgct tcggaacagc tggatttgac tattgatccg gagagtaaag
    20641 ggattcggat tatcacactg cgttcgccgc tgtatgtgcg tgggacgttt aaaaatccgc
    20701 aggctggggt gaaagccgga ccgctgattg cccgtggtgc tgttgctgcg gcactggcaa
    20761 cgctggtaac accggcggcg gcgttactgg cactgatctc accttccgaa ggggaggcta
    20821 atcagtgtcg gacgatattg tcgcagatga agaagtgatg ataattgccg gatgtctgac
    20881 atccggcaat taaacagatt tagatttttg gcaaagtgtg ctttgttcat gccggatgcg
    20941 gcgtgaacgc cttatccggc ctacaaatcc ttgtgaattc aatatattgt atggaaattc
    21001 agtcctgata agcgtagcgc atcaggcttt tcacacttat tacaacgact gatgtcgcgt
    21061 ctcatgggtc agcagcaggg cgattaacgt caagccagcc atcgccgcca gatataaccc
    21121 caccgcacct aacccgtagt tagcctgcaa ccaggctgcg atatacggcg caacggaagc
    21181 cccgagaatc gacgatacgt tgtaagagaa cgatgctccg gtgtaacgca cttctgtcgg
    21241 aaacagctct ggtaacagcg cacccatcgg cccgaaggtc agccccatca gacttaaccc
    21301 cagcagcagg aaggcaaaaa ccagaatcgg gttgccagaa ccgagcagcg ggttaaaggc
    21361 gaacagcgcg aacaggatga tcagcgtggt gatgatcacc atgcttttac ggcgaccaaa
    21421 ggcatcagcc agtaatccgg cgactggcac catcacgcca aaaccaataa ctgccatcat
    21481 cagcatccac aacacttcgt tacgcggcag gccaagccca actggcgcgg cggcggtact
    21541 gaaggtcata gagtagaccg tcatgatgta aaacagcgta taggttgcca gcataatgaa
    21601 cgtacccagt acggttacgc gaacatgttt ggtcagcagc gtacccagcg ggatcttcac
    21661 ctgttttttc gctttagcga ctttctcaaa caccggcgac tcatgcagcg acacgcgaac
    21721 atacaggccg ataatgacca gcaccgccga gaagataaat ggcacacgcc agccccagct
    21781 cataaactgc tcatcggtca gtagccagga aagcagcaag aaagtgccgt tggcaaagaa
    21841 gaagccaatc ggtgcgccta gctgtgggaa ggagccatac agtgcacgtt tgcgcggtgg
    21901 ggcgttttca gtcgccagca gcgccgcgcc gccccattca ccgcctaagc ccagaccctg
    21961 accaaatcga gccagcgcca gcagcagtgg ggcgaaaata ccaatcgtgg catagcccgg
    22021 cagcagacca atcaccacgg tcgaaatccc catcgttagc aacgaggcga ccagcgtcgc
    22081 tttacgccca acgcgatcgc caaaatgacc aaaaacggca gagccaatgg ggcgcgcgac
    22141 gaaggcgatg gcgaaggtgg cgagcgactg tagcgttgct gccgttggat cgccctgcgg
    22201 gaagaagata tgcggaaaca caataacggc cgcagtggcg taaatgtaaa agtcgaagaa
    22261 ctcaatggcc gtgccaatga gagaggcgac aaggacttta ttacgcgagt tgatcggcgt
    22321 gtattcttgt tcgtggtcga gtgttgtggc tgttgcttgc ataatctttt cttatttttt
    22381 ggctaacgaa tagccatatt acgcacagca aaagtgatat ttcaatgtgt aacaaatcgc
    22441 ggaaatggtc aaaaaccagg caaaaagggg ataatgttta ggccagtgat ttttcttcta
    22501 tgaaatgctt cacataattt tataaatagt ggataaaact ggtttcaggt taaataaaag
    22561 ttaccaactg gatttatatg ctggggttat gagttggagt gaaattgttt gctcccaaat
    22621 agatggctga agaggctctg gggaagagtt tcaagaggtt atgtgcgcat tatagccgga
    22681 tgcgatgctg gcgcatctta tccggcctac ggggagccag gccgaataaa atcgtatgcc
    22741 ggataaggcg tttacgccgc atccggccta ttttaaggct gcgttttccc tggcattcgc
    22801 gggtcgtctt tatattcggc ggtggcaatc cacgccgcgc aaaacagcgt caaacgggcg
    22861 aagaagtaga aaaatgccat cagccccagc acggaaccaa atgctgcgcc agagggggat
    22921 ttcatcagcg atggcagggt gtaggtcatc acgattttaa tcacttcaaa accaatagca
    22981 gccagaaatg ttccgcgaat cagggctttt ttgcgcggac ggtggcgcgg caaccgccag
    23041 aagatccaga agaaaagcag atagttggcg aagatggaaa tcgccaggcc aatcaatcgc
    23101 cacgtcggtt tcagccactc aatgctgttc aggtgcaggg cgctaataat catttgctgc
    23161 gccgaaccgg caaccgaggt gatcgaaagc gtcacaatca gcgcaatcaa caaaccaatc
    23221 agtgaaataa aatcacgcag atattttacc cagaacttct cctgatcttg cggcgagcgt
    23281 tcccagacat cgcgcgactg ggcacgaatc gcttcacgca ggttacccat ccagttgatg
    23341 ccggaataaa gtgccaccgc caggccgaca agccctacag tcgtacgctg ctgaacggcg
    23401 gtgttgatgg tgtttttcaa cgtggcggct agtgtcggat cgctgatgtt ttgcagaatt
    23461 ttgtcgaaga tatcctgtag caacatcgga tgggaggcca gcacaaagcc ccctgcggca
    23521 aacgacacca tcaaaatcgg gatcatcgac aaaaatgaga aataggtgat agccgcacca
    23581 aactggttac ccagccgatc gttaaagcgt tctgtcgcgc gaataaggtg cgcaataacc
    23641 ggttggcgtt ggactttttc ggcagtggtg gtgacggttt ccagcgcctg gctaactttg
    23701 ccgcctttct cgctattatc caactgctta atcggctcgt gctccagatc ctgggtggga
    23761 cgtttgatct cgttttcctg cgtcatccgc tcttttgtcc ttctgtcgtg gtcattctta
    23821 aaaagtatag tcggtcagtc caggtgctct ttcattacgc cagccagcca ctgcataaac
    23881 aggtttacac gccgggaaag ctcccgacgc tgcgggtaaa ccagcgaaag ggagagcggc
    23941 tcggcacggt agcctggcaa cacttcaata agccgcccgg cacgcagggc ttcgcgcacg
    24001 gcaatgcgcg ggatctgaat aatccccaga ccggcaagac cggcggcgag atagttttcg
    24061 ctactgttta ccgtcaacat gccgccagac ttaaaccact ggacgccatt aacgctggca
    24121 acctcaaaac ctaacggatg tacacccagg tgcggtgtat aacgcactat tgcgtgtgaa
    24181 gtcagatcat cggggctttg cggataacca aagcgcgtca gatagtgcgg actggcacag
    24241 ttgaccatgg ttagtttgcc gaggggacgg gcgataacgc catcttccgg taacgcgcca
    24301 gtgcgtatca cgcaatcaaa accatcatga agaatatcta ccggacggtt atggctactc
    24361 agttccagct caattcccgg atgctgatag agaaattccg acagacgcgg cagtaacagg
    24421 tttttcgcga ttcctgacgg gatgtcgatg cgtaatttac cgctgatact ggtggcatcc
    24481 tgttgaaaca gaccgtccag ttcgctgaga ttactcaaca catcttttgc ccgttgataa
    24541 taggtcatgc cttctggcgt gagtttgacc cgtcgcgtag tgcgctggag aagctgggtg
    24601 ccaagttgat gttccagtgc ctgtatctgg cgcgaaacac ttccctttgg caaagcaaag
    24661 aaatccgccg cgcgggaaaa actttccagc tccgcgactt tgatgaacaa ctgcattgcg
    24721 tgaattttat ccatttcttt gaccgattgt tgttcacagc gaaacagtaa cgcgcatatc
    24781 gcattgttta ttgttttttc taacatctaa tgccgggttt cacaatgttg tcatgacgaa
    24841 tgtggcgatt tctcagcagg aaaagggcgg aatagcttta tgagaaaggt gaatcccctt
    24901 aaacagagcg tatttttcag tgattccctt taaagtgaat agaggacgta ttggctattt
    24961 gtgcttattt ttgtctcatt gccatttgtt attttggctt gtagtcgcgg aatttcattt
    25021 ttaattgcat gcgtactaac caccaatata aggaaatcct caaaattgtt ttaaatagtg
    25081 ccctcgacat gtaatttcag gcgtttagct gtaactattt atatcggtaa ctattttcta
    25141 cgcgtcatct ctgtagaatg gccaccgcca aatggcgaat tcttattttt tctcgcgggt
    25201 aatacccttc cactacttaa gtctttttaa cgtattcagg tggagattca gttatgcaaa
    25261 tagtcatgtt tgacaggcag tcaatattta ttcatggaat aaaaatcagt ttacagcagc
    25321 gtattccagg agtgagtatt cagggggtca gtcaggcaga cgagttatgg caaaagctgg
    25381 aaagttaccc tgaagcctta gttatgctcg atggcgatca ggatggtgag ttttgctact
    25441 ggttgctgca aaaaaccgtg gtgcagtttc ctgaggttaa ggtgttaatc acggcgacgg
    25501 attgcaataa acggtggtta caggaagtta tccattttaa tgtgctggcc attgtgcctc
    25561 gcgattcaac cgtcgagaca tttgcgttgg cggtaaatag tgcggcgatg gggatgatgt
    25621 ttctgcctgg agactggcgg acgacgccgg aaaaggacat taaagatctc aaatcgttaa
    25681 gcgcccgcca gcgggagatt ttaaccatgt tagcggcggg cgaatcaaat aaggagatcg
    25741 gcagagcgct gaatatcagt accggaacgg taaaagcgca tctggaatcc ctgtatcgcc
    25801 gtctggaagt gaaaaatcgc acccaggcgg caatgatgtt aaatatctcc tcctg
//
LOCUS       DAJTSF010000055        25743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000055 DAJTSF010000000
VERSION     DAJTSF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.055"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..122)
                     /locus_tag="OO335_002366"
     CDS             complement(<1..122)
                     /locus_tag="OO335_002366"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6002726.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVA"
     gene            complement(255..560)
                     /locus_tag="OO335_002367"
                     /pseudo
     CDS             complement(255..560)
                     /locus_tag="OO335_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            628..1243
                     /gene="tnpA"
                     /locus_tag="OO335_002368"
                     /pseudo
     CDS             628..1243
                     /gene="tnpA"
                     /locus_tag="OO335_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1571..2653
                     /locus_tag="OO335_002369"
     CDS             1571..2653
                     /locus_tag="OO335_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR6002727.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3142..3940)
                     /locus_tag="OO335_002370"
                     /pseudo
     CDS             complement(3142..3940)
                     /locus_tag="OO335_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3950..4501)
                     /gene="ybcL"
                     /locus_tag="OO335_002371"
     CDS             complement(3950..4501)
                     /gene="ybcL"
                     /locus_tag="OO335_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR6002728.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVAYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(4670..5002)
                     /gene="emrE"
                     /locus_tag="OO335_002372"
     CDS             complement(4670..5002)
                     /gene="emrE"
                     /locus_tag="OO335_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR6002729.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5347..5661)
                     /gene="fliE"
                     /locus_tag="OO335_002373"
     CDS             complement(5347..5661)
                     /gene="fliE"
                     /locus_tag="OO335_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6002730.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5875..7534
                     /gene="fliF"
                     /locus_tag="OO335_002374"
                     /pseudo
     CDS             5875..7534
                     /gene="fliF"
                     /locus_tag="OO335_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            7527..8522
                     /gene="fliG"
                     /locus_tag="OO335_002375"
     CDS             7527..8522
                     /gene="fliG"
                     /locus_tag="OO335_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6002731.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            8515..9201
                     /gene="fliH"
                     /locus_tag="OO335_002376"
     CDS             8515..9201
                     /gene="fliH"
                     /locus_tag="OO335_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6002732.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            9201..10574
                     /gene="fliI"
                     /locus_tag="OO335_002377"
     CDS             9201..10574
                     /gene="fliI"
                     /locus_tag="OO335_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR6002733.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            10596..10937
                     /gene="fliJ"
                     /locus_tag="OO335_002378"
                     /pseudo
     CDS             10596..10937
                     /gene="fliJ"
                     /locus_tag="OO335_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            10934..12061
                     /gene="fliK"
                     /locus_tag="OO335_002379"
     CDS             10934..12061
                     /gene="fliK"
                     /locus_tag="OO335_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6002734.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            12166..12630
                     /gene="fliL"
                     /locus_tag="OO335_002380"
     CDS             12166..12630
                     /gene="fliL"
                     /locus_tag="OO335_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6002735.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            12635..13639
                     /gene="fliM"
                     /locus_tag="OO335_002381"
     CDS             12635..13639
                     /gene="fliM"
                     /locus_tag="OO335_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6002736.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            13636..14049
                     /gene="fliN"
                     /locus_tag="OO335_002382"
     CDS             13636..14049
                     /gene="fliN"
                     /locus_tag="OO335_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6002737.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            14052..14417
                     /gene="fliO"
                     /locus_tag="OO335_002383"
     CDS             14052..14417
                     /gene="fliO"
                     /locus_tag="OO335_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6002738.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            14417..15154
                     /gene="fliP"
                     /locus_tag="OO335_002384"
     CDS             14417..15154
                     /gene="fliP"
                     /locus_tag="OO335_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR6002739.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            15164..15433
                     /gene="fliQ"
                     /locus_tag="OO335_002385"
     CDS             15164..15433
                     /gene="fliQ"
                     /locus_tag="OO335_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6002740.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            15442..16227
                     /gene="fliR"
                     /locus_tag="OO335_002386"
     CDS             15442..16227
                     /gene="fliR"
                     /locus_tag="OO335_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR6002741.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            16512..17135
                     /gene="rcsA"
                     /locus_tag="OO335_002387"
     CDS             16512..17135
                     /gene="rcsA"
                     /locus_tag="OO335_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR6002742.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(17179..17367)
                     /gene="dsrB"
                     /locus_tag="OO335_002388"
     CDS             complement(17179..17367)
                     /gene="dsrB"
                     /locus_tag="OO335_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6002743.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            17530..17757
                     /gene="yodD"
                     /locus_tag="OO335_002389"
     CDS             17530..17757
                     /gene="yodD"
                     /locus_tag="OO335_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR6002744.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            18055..18870
                     /locus_tag="OO335_002390"
                     /pseudo
     CDS             18055..18870
                     /locus_tag="OO335_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(18867..20561)
                     /gene="yedQ"
                     /locus_tag="OO335_002391"
                     /pseudo
     CDS             complement(18867..20561)
                     /gene="yedQ"
                     /locus_tag="OO335_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(20732..20914)
                     /locus_tag="OO335_002392"
     CDS             complement(20732..20914)
                     /locus_tag="OO335_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6002745.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(20993..21910)
                     /locus_tag="OO335_002393"
     CDS             complement(20993..21910)
                     /locus_tag="OO335_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6002746.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            22083..23003
                     /gene="yedA"
                     /locus_tag="OO335_002394"
     CDS             22083..23003
                     /gene="yedA"
                     /locus_tag="OO335_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR6002747.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(22992..23462)
                     /gene="vsr"
                     /locus_tag="OO335_002395"
     CDS             complement(22992..23462)
                     /gene="vsr"
                     /locus_tag="OO335_002395"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6002748.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(23443..24861)
                     /gene="dcm"
                     /locus_tag="OO335_002396"
     CDS             complement(23443..24861)
                     /gene="dcm"
                     /locus_tag="OO335_002396"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6002749.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(24928..25468)
                     /locus_tag="OO335_002397"
                     /pseudo
     CDS             complement(24928..25468)
                     /locus_tag="OO335_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(25508..>25743)
                     /locus_tag="OO335_002398"
     CDS             complement(25508..>25743)
                     /locus_tag="OO335_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002750.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6492 a         6602 c         6342 g         6307 t
ORIGIN      
        1 gccacgccag catccagccc gaccattgat gctgaagggt gaaccggagt tgatacttca
       61 ctttctgcct gaatactgat gtaccactta ccgcaggact ggctgacagt gacatttttc
      121 acaacacccg tgacctgacg gctgttccgg tagcgcatcc agccaagttt tggcagaaaa
      181 atacggctgt tttcctgagc gagcttaacg ccctgcggat agcggaatgc atcattctgt
      241 caccgctttt tgaatcaggg gaacgccgag cgcttctgga aaaagttttt gcaggcctgc
      301 tcagggtctt tcagtgactg ttgcaatggc tgtgacggag catctttaat ccattgcgtt
      361 tcagtggcat ttttccactc aaccaatcag gaagccattt tcgcgtaagg gatgtctttg
      421 ttaccggcct tatgattctc attctgacgt gccagagtac gactgaaaac gaaacgacat
      481 gcgcctgcga agcgcctcat ttcacgcgcc tgttgaccac cgggtcttaa ccggaatttg
      541 aatacctgta gtcgtttcac agggcaaatt atactttggt ctgtgaaaaa aactgatatc
      601 cgacgcggaa ggcactgtgt tttcctgatg catgctcacc gggtctttgt taccaagtac
      661 cgacgccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt ttcaaatgta
      721 tgtgcttatt ttgaagctga actggttgaa atggatggcg aaccagatca cgtccatctg
      781 ttaattaact atccccccaa actggcgata tccagtctgg taaacaaaca gcctcaaagg
      841 cgtatcgggt aggttactgc gacgggatcg accagatatt gctgccaggt attaccgcaa
      901 agatgttttg tggcgtcccg gctattttgc cagtagctgc ggaggtgcgc caatatccgt
      961 catccgccaa tacattgaac aacagcaaac acctggtcag atgaaaaaac gagccttata
     1021 tccccgccct gaaggacggg cttttacggt gcactggata agcctggaga ttatctacac
     1081 ctgcatggac atcattgtac tatgccccgt acatgctcca tcacatcctc aacatcaaag
     1141 tattagagcc ttgcaaacca ttatgcgtct cagtttgtct catctatctt acgcaaaaaa
     1201 aatagaacta atatcaagcg gcgctatatt gcgtattaaa taaaaccacc tcaattacat
     1261 taataaatct aaataaatat catgcaaata atttccatgg ttatttttag gctatttaaa
     1321 ttaaaaaaat aaattatgag tcaatcataa ataacaacct ttaatataat ttgactacca
     1381 aatttatatt cgttcacaaa atagtcatgc attgtgtgca atagaaacag ttcagataaa
     1441 aatagggatc tacttcacaa tttaaatgag aaactaaaac ttacatcttg aaataatctc
     1501 attgattgga tgaatatttg tagtgcagtg acatcatttt taaataatag ttcaaataaa
     1561 agggctcacg atgaaaaaat taacagtggc aatttctgct gtagctgcat cagtactgat
     1621 ggcgatgtct gctcaggcag cagagattta caataaagac agtaacaagc tggatctgta
     1681 cggaaaagtt aatgctaagc actacttctc ttctaacgat gcagatgatg gcgacacaac
     1741 ttatgttcgt ctgggcttca aaggcgaaac tcaaatcaac gatcaactga ctggtttcgg
     1801 tcagtgggaa tatgaattca aaggcaaccg tgctgaatct caaggttctt ccaaagataa
     1861 aacccgtctt gccttcgctg gcctgaaatt cggtgactac ggctccatcg attatggccg
     1921 taactacggt gtagcatacg atatcggtgc atggaccgac gttctgccag aattcggtgg
     1981 cgatacctgg acccaaacag atgtgttcat gactggtcgc accacaggtg ttgcaaccta
     2041 tcgtaacaac gacttctttg gtctggttga tggtctgaac tttgctgctc agtatcaggg
     2101 taaaaatgac cgcactgacg taactgaagc taacggtgat ggtttcggtt tctccactac
     2161 ttatgagtat gaaggattcg gtgtaggtgc aacctatgct aaatctgacc gcactaatga
     2221 tcaggttatc tacggtaaca acagcctgaa tgcatctggt caaaatgctg aagtatgggc
     2281 agctggtctg aaatatgatg cgaacaacat ctatctggct accacctatt ctgaaaccca
     2341 gaacatgact gtttttggta ataaccatat tgccaacaaa gcacaaaact tcgaagtagt
     2401 tgcacaatat cagttcgact tcggtctgcg tccgtccgtt gcttacctgc aatctaaagg
     2461 aaaagacttg ggtgcgtggg gtgatcagga cctgattgaa tatattgatg taggtgcaac
     2521 ctattacttc aacaaaaata tgtccacttt tgttgattac aaaatcaacc tgattgataa
     2581 gagcgatttc acgaaagcat ctggcgttgc taccgatgat atcgttgctg taggattggt
     2641 ttaccagttc taatctgatt actcaaagat atgttgtggg aggctttgcc tccccaacat
     2701 ataagtggcg ctctcaagcc actttcctta gaagcactat cttgcttctg actatataaa
     2761 ttacccttta ttttctggga cgtttcaacg ccccattttt aataatactc agtgaacaat
     2821 taacgtatca attagattta ataacaacga tatccgtacc taacctgata tctaatgtca
     2881 taagcatccc tttaattatc ccctcaacct cttgtaaatt tcccactctt gagctccaca
     2941 tagcccaaac gccggatgca aaaaaaatca tacgtgcatc tctgaaattg tgatggagga
     3001 agtgagtatc aattatatgt gcttataaac gttcccgatt ggcacagaag tcaccggatt
     3061 gttcaggctc caataggttg agtatgtcgt atttattttc caaaataaaa atcgatgctg
     3121 ttgctcctta ataaaatact tttactctct ggacgctttt cttagctctc ttggtgttat
     3181 attaaaacga ttatggaacc tttcagtaaa acgagaagga cacttgtaac cattttccct
     3241 ggcaatctcg ctgatgggtt ttactgtcgt ttgtatagca ggacaacgca ttatttaacc
     3301 tcacgtcgtc caatatactt tgaaaactta ttccctcgct tgccagacgg cgatgcaatg
     3361 tggaaacaga aatgtagaga tatctcgcta ccttgtttgc tgtccatttt gttctaggtt
     3421 cggatagcat taggttataa caacgactta ttaatgattg tttactatct gataaaagta
     3481 aacaattaac atgatgtgct cctaatgaaa gtagaacgcc cattgctaag tgttcctgaa
     3541 tttgcgttga gaagcctcgg gaaatagatg tttttagttg ctcccaacaa tatattaact
     3601 cggggttctg aggtaagcag aaacctgtcg tgttacgaat ttgatcagtt atcgcataaa
     3661 atttttgaaa cttttcaatt agatcaatgg gataataaag catttctgca agataaagac
     3721 ctgcttctgg ataattcaca atataaaatt catatccaca gggaaataat attatctgat
     3781 tattatcaac agttagacta tgcgtctccc aattgataac tttttttccc tgacggatac
     3841 gacataaagc tggcataaga ggcttaaccc tgtgaatctc atgatgttta tgcatccgta
     3901 tttcttcaat ctttaggtta gacttatctc ttgccagcat actcttaccc tattttatct
     3961 cataaactgg tgttatctca gcggttgcaa ttttattggc attaagcata taaccaacta
     4021 acgctccgct ggaattagaa tccacaggaa tcttatcagt ttttagagcc catactttaa
     4081 actggtaatg atggggttta tctcctttgg gaggacatgc tccaccaaat cctgcataac
     4141 caaaatcatt tcggccttga acagcaccag tcggaagttt tgtaccatca cgtcttcctg
     4201 catcagtggg caaatatgct acagttgctg gaatattagc aacagtccag tgccaccatc
     4261 cactgcctgt aggagcatct ggatcataca cagttacggc aaagcttttg gtgccttcag
     4321 gagcaccaga ccaggttaat gagggcgatg tattaccacc ttcacaccca aatccagaaa
     4381 atacatgaga cgttgtaagt tgctctcctg tttttatttc attactagtg acctgaaatg
     4441 ctgcggcctg cgcagaaaat gttatgaatg ccaatacact tgaaacgata agttttttca
     4501 taaaaacctc attgttgtga cctatcgtta ttttatttga tgtgccttga tctcattatg
     4561 cataaaggcg caatgatcgc gcaaatgcaa tcataattat aaaggtgccc cgaacattag
     4621 ccataatatg cacaaatcag attcctgtta tctcaaggag aattattttt taatgcggtg
     4681 cacttcgtga caacaagtta atgactaaca caccggcaca aatcaacatc atgcctatga
     4741 tagctggcag gtccagtcgt tgaccaaaaa atccccagga aagcaaacta atcagaacga
     4801 taccgactcc tgaccagata gcataagcaa tccctgtagg tatataagcc agcgtctgag
     4861 ctaataacca gaatgatgca caatagcaaa taattgtgcc aacagagggc cataaccgtg
     4921 taaacccctc tgaaaacttc attaaggttg taccaatgac ctctgcaagt attgcgccac
     4981 caagataaat ataagggttc atagcatatt ctttcctgtt catactggag agaattgtac
     5041 tacagtttga acacaactca ccgttttcat cctggtgtat gtgtatccaa ggggctctgg
     5101 cgtggcaaga gataaaggca ggaacaataa tgatcagaag gaaccccagg ctaacgaatg
     5161 agttcactct tcctgggagc aaagcgaaga caaaacatgt tatcgcgttt acggatcgtg
     5221 gatggcagaa aacaaccagg atcaggtatc cattttaaac cagaaattga gtgagtttgc
     5281 cccatccatg ccccacgctg tgggatcgga tataattaaa caagcatagt caacaggtta
     5341 cacaacctac acctgcatgg acatcacttc ctgatacgcc gccaccagct tattacgcac
     5401 ctgaatcccc atttgcatag aaactgaggc tttttgcata tcggtcatca catcgtttag
     5461 cgccacgccg ggttcaccga gggtgaattt ttctgcctgc gtgcgggcga ctgtttgtgt
     5521 atcgcttatg cgatcgagcg cggcgtgcag ctgcccggca aaactaatgg tcggttgcgg
     5581 cagtgattcc tgcgcacgcg cactcatcgc cgtagcctgt aactggctga taaccccttc
     5641 aatcccctgt atcgctgaca ttttcgtctc ccggataatt tctggtaaca aagcctacca
     5701 gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa actggtttat tggcggatag
     5761 aaaaaacgaa agcacaaata atgggagcgt caatttttcg agtgtgctga cccgagagtg
     5821 agtcttgttc cactttgcca ataacgccgt ccataatcag ccacgaagtg cgcgatgaat
     5881 gcgactgcag cccagacaaa atctcttgag tggcttaatc gcctgcgtgc gaatccgaaa
     5941 attccattga ttgttaccgg ttccgcggca gtggcggtca tggtcgcact gatcctgtgg
     6001 gcgaaagccc ccgactaccg cacattattc agcaatcttt ccgatcagga tggtggcgca
     6061 attgtcagcc aactgacgca aatgaatatt ccttaccgct tcagcgaagc cagcggcgct
     6121 attgaggttc cggcagataa agttcacgaa ctgcgcctgg cgcaacaagg tttgcccaaa
     6181 ggcggcgcgg tcggtttcga actgctcgat caggaaaagt ttggtatcag ccagttcagc
     6241 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
     6301 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
     6361 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
     6421 gaagggcaaa ttagcgctat tgtacatctg gtttccagcg cggttgctgg tctgccgcct
     6481 ggaaacgtca cgctggtgga tcagggcggg catctgttaa cccagtccaa taccagcggg
     6541 cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcag
     6601 cgtattgaag cgatcctgtc acctattgtt ggtaacggta atattcacgc ctaggtcacg
     6661 gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
     6721 gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
     6781 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
     6841 atcagcacgc ctccgacaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
     6901 agtgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
     6961 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat
     7021 tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt
     7081 gaagctctga cccgcgaggc gatgggcttt tctgaaaaac gcggtgattc gctcaatgtt
     7141 gttaactcgc cgttcaatag cagtgacgaa agcggcggag cactgccgtt ttggcaacag
     7201 caagtgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggttgc tggtactgct
     7261 ggtggcgtgg ttgctgtggc ggaaagcggt acgcccgcag ctaacacgtc gcgccgaggc
     7321 ggtgaagact gtgcagcaac aggcgcaggc ccgcgaggaa gtggaagatg cggtggaagt
     7381 ccgtctgagc aaagacgaac aactccaaca acggcgcgct aaccaacgac tgggggcaga
     7441 agtcatgagc cagcgtatcc gtgaaatgtc tgataacgat ccgcgcgtgg tggcgctggt
     7501 cattcgccag tggataaata acgatcatga gtaacctgac aggcaccgat aaaagcgtca
     7561 tcctgctgat gaccattggc gaagaccggg cggcagaggt gttcaagcac ctctcccagc
     7621 gcgaagtgca aaccctgagc gctgcaatgg cgaacgtcac gcagatctcc aacaagcagc
     7681 taaccgatgt gctggcggag tttgagcaag aagctgaaca gtttgctgca ctgaatatca
     7741 acgccaacga ttatctgcgt tcggtattgg tcaaagctct gggtgaagaa cgtgccgcca
     7801 gcctgctgga agatattctc gaaacccgcg ataccgccag cggtattgaa acgctcaact
     7861 ttatggagcc gcagagcgcc gccgatctga ttcgcgatga gcatccgcaa attatcgcca
     7921 ccattctggt gcatctgagg cgcgcccagg ccgccgatat tctggcgttg ttcgatgaac
     7981 gtctgcgcca cgacgtgatg ttgcgtatcg ccacctttgg cggcgtgcag ccagccgcgc
     8041 tggccgaact gaccgaagta ctgaatggct tgctcgacgg tcagaatctc aagcgcagca
     8101 aaatgggcgg cgtgagaacg gcagccgaaa ttatcaacct gatgaaaacg cagcaggaag
     8161 aagccgttat taccgccgtg cgtgaattcg acggcgagct ggcgcagaaa atcatcgacg
     8221 agatgttcct gttcgagaat ctggtggatg tcgatgatcg cagcattcag cgtctgttgc
     8281 aggaagtgga ttccgaatcg ctgctgatcg cgctgaaagg ggccgagcag ccactgcgcg
     8341 agaaattcct gcgcaatatg tcgcagcgtg ccgccgatat tctgcgcgac gatctcgcca
     8401 agcgtggtcc ggtgcgtctg tcgcaggtcg aaaacgaaca gaaagcgatt ctgctgattg
     8461 tgcgccgtct tgccgaaact ggcgagatgg tgattggcag cggcgaggat acctatgtct
     8521 gataatctgc cgtggaaaac ctggacgccg gacgatctcg cgccaccacc ggcagagttt
     8581 gtgccaatgg tcgagtcgga agaaaccatt attgaagagg ccgaacccag ccttgagcag
     8641 caactggcgc aactgcaaat gcaggcccac gagcagggtt atcaggcggg gattgccgaa
     8701 ggtcgccagc aaggtcacga acagggctat caggaaggac tggcccaggg gctggaacaa
     8761 ggtctggcag aggcgaaggc tcaacaagcg ccaattcatg cccggatgca gcaactggtc
     8821 agcgaatttc aaactaccct tgatgcgctt gatagtgtga tagcttcgcg cctgatgcag
     8881 atggcgctgg aggcagcacg tcaggtgatc ggtcagacac caacggtgga taactcggca
     8941 ctgatcaaac agatccaaca gttgttgcag caagaaccgt tattcagcgg gaaaccacag
     9001 ctgcgcgtac acccggatga tctgcaacgt gtggatgata tgctcggcgc aactttaagt
     9061 ttgcatggct ggcgcttgcg gggcgatccc accctccacc ctggcggatg taaagtctcc
     9121 gccgatgaag gcgatctcga cgccagtgtc gccactcgct ggcaagaact ctgccgtctg
     9181 gcagcaccag gagtggtgta atgaccacgc gcctgactcg ctggctaacc acgctggata
     9241 actttgaagc caaaatggcg cagttgcctg cggtacgtcg ctacgggcga ttaacccgcg
     9301 ctaccgggct ggtgctggaa gccaccggat tacaattgcc gctcggcgca acctgtgtca
     9361 ttgagcgcca gaacggcagc gaaacgcacg aagtagaaag cgaagtcgtt ggctttaacg
     9421 gtcaacggct gtttttaatg ccgctggagg aagtggaagg tgtcctgccc ggcgcgcgtg
     9481 tatatgccaa aaacatttcg gcagaagggc tgcaaagcgg caagcagttg ccgctcggtc
     9541 cggcgttatt aggtcgtgtt ctggacggta gcggtaaacc gctcgatggc ctgcccttcc
     9601 ccgatacgac ggaaaccggt gcgctgatta ccccgccatt taacccgttg caacgtacac
     9661 cgattgaaca tgtgctggac accggcgtgc gcccaatcaa tgccctgctt accgtcggac
     9721 gcgggcagcg tatggggctg tttgccgggt cgggcgttgg taaaagtgtg ctgctgggga
     9781 tgatggcacg ctacacccgc gccgatgtca ttgtcgtggg tttgattggt gaacgtgggc
     9841 gcgaagtaaa agattttatt gagaatatcc tcggtgccga aggacgtgca cgctcagtgg
     9901 tgattgccgc tccggcggat gtttctccgc tcctgcgaat gcaaggtgcc gcctatgcca
     9961 cgcgcattgc cgaagatttt cgcgatcgtg gtcagcatgt gttgctgatt atggactccc
    10021 tcacccgcta cgcgatggcc cagcgtgaaa ttgcgctggc gattggcgaa ccacctgcca
    10081 ctaaaggtta tccaccgtcg gtgtttgcca aattaccggc actggtcgag cgtgccggta
    10141 atggcattag cggcggcggc tcgattaccg cgttttatac cgtgctcacc gaaggcgatg
    10201 accagcagga ccccattgct gactccgcgc gggcaatcct cgacggtcac attgtgctgt
    10261 ctcgccgact ggcggaagcc gggcactatc cggctatcga tattgaagcg tcgatcagtc
    10321 gcgcaatgac ggcgttgatc agtgagcaac attacgcgcg agtgcgcacc ttcaaacagc
    10381 tgttgtcgag ttttcagcgt aaccgcgatc tggttagcgt cggcgcgtat gccaaaggca
    10441 gcgatccgat gctcgataaa gccatcgccc tgtggccgca gctggagggc tatttgcaac
    10501 aaggcatttt tgaacgcgcg gactgggaag cgtctctcca ggggctggag cgtattttcc
    10561 cgacagtgtc ataacccagg agataacggc agatggcgga agaacagctc aaaatgttga
    10621 tcgattatca gaatgaatat cgcaataacc tcaatagcga tatgagtgcc gggatgacca
    10681 gcaaccgctg gatcaactat cagcagttta tccagacgct ggaaaaagcc attactcagc
    10741 atcgccagca acttaatcag tggacgcaga aagttgacat tgccctgaac agctggcgag
    10801 aaaagaaaca acgtttgcag gcctggcaga cactgcagga acggcaatcc acggcggcac
    10861 tgcttgcaga aaaccgcctc gatcagaaaa agatggatga gttcgcccag cgcgccgcca
    10921 tgaggaaacc tgaatgattc gcttagcgcc cttgattacc gccgacgttg acaccaccac
    10981 attgcctggc ggcaaagcca gcgatgctgc acaagatttt ctcacgttgt tgagcgaagc
    11041 attagcaggc gagacaacaa ctgacaaagc ggccccccag ttgctggtag caacagataa
    11101 gcccacgaca aaaggcgagc cgctggtcag cgatattctt gccgatgcgc aacaagcgga
    11161 tttactgatc cctgtggatg aaacacttcc tgtcatcaac gacgaacaat ccacatcaac
    11221 accgttaacc accgctcaga cgatgacgtt ggctgcggtg gctgacaaaa atacgacaaa
    11281 agacgaaaaa gcggatgatc ttaatgaaga cgtcaccgca agtctgagcg ccctttttgc
    11341 gatgttgccg ggttttgaca atacgcccaa agtgactgat gcaccgtcaa ccgtgttacc
    11401 ggcagagaaa ccaacgctct tcacaaaact gacttctgcg caactcacta cagcacagcc
    11461 tgatgacgcc cccggcacac cagctcagcc attaacaccg ctggtagcag aagcccagag
    11521 taaagcggaa gtcatcagca cgccttcgcc ggtgaccgct gacgccagcc cgctaatcac
    11581 tccacaccag acacagccac tgcccaccgt cgccgcgcct gttttgagtg caccgctggg
    11641 ttctcacgaa tggcaacaat cattgagcca gcatatttcg ctgttcaccc gccaggggca
    11701 acaaagtgca gagttgcgtc tgcacccaca ggatttaggt gaagtgcaaa tttccctcaa
    11761 agtggatgat aaccaggcgc agatccagat gatttcaccg catcagcacg tacgcgccgc
    11821 cctggaagca gcgctgccgg tactgcgtac gcagctggcc gaaagtggca ttcagttagg
    11881 gcaaagcaac attagtggcg aaagctttag tggtcagcag caggccgctt cccagcaaca
    11941 gcaaagccaa cgcacagcaa accatgaacc tctggcgggg gaagacgacg atacgcttcc
    12001 ggttcccgtc tctttacaag ggcgtgtaac aggtaacagc ggcgttgata ttttcgccta
    12061 acgtcagagg tagcaccgta atccgcgtct tttccccgct ttgttgcgct caagacgcag
    12121 gataattagc cgataagcag tagcgacaca ggaagaccgc aacacatgac tgattacgcg
    12181 ataagcaaga aaagcaagcg atcgctttgg atcccgattc tggtattcat taccctcgcg
    12241 gcctgtgcca gcgcaggtta cagctactgg cattcgcatc aggttgccgc tgacgacaaa
    12301 gcgcagcaac gcgtcgtgcc ctccccggtc ttctatgcgc tggatacctt tacggtcaat
    12361 ttgggcgatg cggatcgcgt gctttatatc ggcataaccc tgcgactgaa agatgaagca
    12421 acccgctcgc ggctgagtga gtatttgccg gaagtccgta gtcgcttgct gttactgttt
    12481 tcacgtcagg atgctgccgt actggcgaca gaagaaggca agaaaaacct gattgccgag
    12541 attaaaacca cactttccac cccgcttgtt gccgggcaac cgaaacagga tgtcaccgac
    12601 gtgctgtata ccgcttttat tctgcgataa cgacatgggc gatagtattc tttctcaagc
    12661 tgaaattgat gcgctgttga atggtgacag cgaagtcaaa gacgaaccga cagccagtat
    12721 tagcggcgaa agtgacattc gtccgtacga tccgaatacc caacgacggg ttgtgcgcga
    12781 acgtttgcag gcgctggaaa tcattaatga gcgctttgcc cgccattttc gtatggggct
    12841 gttcaacctg ctgcgccgta gcccggacat taccgtcggg gccatccgca ttcagccgta
    12901 ccatgaattt gcccgcaacc tgccggtgcc gaccaacctg aacctaatcc acctgaaacc
    12961 gctacgcggc acagggctgg tagtgttctc gccgagtctg gtgtttatcg ccgtggataa
    13021 cctgtttggc ggcgatggac gcttcccgac caaagtggaa ggtcgcgagt ttacccatac
    13081 tgaacagcgc gtcatcaacc gtatgttgaa gctggcgctg gaaggctata gcgacgcctg
    13141 gaaagcgatt aatccgctgg aagttgagta cgtgcgttcg gaaatgcagg tggaatttac
    13201 caatatcacc acctcgccga acgacattgt ggttaacacg ccgttccatg tggagattgg
    13261 caacctgacc ggcgaattta atatctgcct gccattcagc atgatcgagc cgctacggga
    13321 attgttggtt aacccgccgc tggaaaactc gcgtaatgaa gatcagaact ggcgcgataa
    13381 cctggtgcgc caggtgcagc attcacagct ggaactggtg gccaactttg ccgatatctc
    13441 gctacgcctg tcgcagattt taaaactgaa acccggcgac gtcctgccga tagaaaaacc
    13501 cgatcgcatc atcgcccatg ttgacggcgt cccggttctg accagtcagt acggtactct
    13561 caacggtcag tatgcgttac ggatagaaca tttgattaac ccgattttaa attctctgaa
    13621 cgaggaacag cccaaatgag tgacatgaat aatccggccg atgacaacaa cggcgcaatg
    13681 gacgatctgt gggctgaagc gttgagcgag caaaaatcaa ccagcgaaaa aagcgctgcc
    13741 gacgcggtgt tccagcaatt tggcggtggt gatgtcagcg gaacgttgca ggatatcgac
    13801 ctgattatgg atattccggt caagctgacc gtcgagctgg gccgtacgcg gatgaccatc
    13861 aaagagctgt tgcgtctgac gcaagggtca gtcgtggcgc tggacggtct ggcgggcgaa
    13921 ccgctggata ttctgatcaa cggttattta atcgcccagg gcgaagtggt ggttgttgcc
    13981 gataaatatg gcgtgcggat caccgatatc attaccccgt ccgagcggat gcgccgcctg
    14041 agccgttagt gatgaataac catgctactg tgcaatcttc cgcgccggtt tctgctgagc
    14101 cactgctgca ggtgagcggc gcactcatcg ccattattgc cctgatcctc gctgctgcct
    14161 ggctggtaaa acggctggga tttgccccta aacgcactgg cgttaacggt ctgaaaatta
    14221 gcgccagtgc ttcgctgggc gcgcgtgaac gggtcgtggt ggtcgatgtg gaagatgcac
    14281 ggctggtgct cggcgttacc gcagggcaaa tcaatctgct gcataaactt cccccttcag
    14341 caccaacgga agagataccg cagaccgatt ttcagtcggt catgaaaaat ttgcttaagc
    14401 gtggcgggag atcctgatgc gtcgtttgtt ttctgtcgca ccggtccttc tctggctgat
    14461 tacgcccctc gccttcgcgc aactgccggg tatcaccagc cagccgctac ctggcggtgg
    14521 acaaagctgg tcgctcccgg tgcagacgct ggtgttcatc acctcgttga cgttcattcc
    14581 agcgatttta ctgatgatga ccagtttcac ccgcatcatt attgtttttg gtttattgcg
    14641 taacgcgctg ggaacgccct ccgcgccacc taaccaggta ttgctggggc tggcactgtt
    14701 tttgaccttt tttattatgt cgccagtgat cgacaaaatt tatgtcgatg cgtaccagcc
    14761 attcagcgaa gagaaaatct caatgcagga ggcgctggaa aaaggggcgc agccgctgcg
    14821 tgagtttatg ctgcgtcaga cccgcgaggc agatttaggg ttgtttgcca gactggcgaa
    14881 taccggcccg ttgcaggggc ctgaagctgt gccgatgcgc attttgctcc cggcctacgt
    14941 gaccagcgag ttgaaaaccg catttcagat aggcttcacg attttcatcc cttttttgat
    15001 tatcgacctg gtgattgcca gcgtgttgat ggcattgggg atgatgatgg ttccccccgc
    15061 caccattgct ctgcccttta aactgatgct gtttgtactg gtggatggct ggcaattgct
    15121 ggtcggttcg ctggcgcaga gcttttacag ctagagaggc aaaatgacac ctgaatcggt
    15181 catgatgatg ggaactgaag cgatgaaagt cgcgctggca ctggcagccc cgctgttgct
    15241 ggtagcgctg gtcacgggcc ttatcatcag tattttgcag gcagccacgc agattaacga
    15301 aatgacgctg tcgtttattc cgaaaatcat cgccgtattt atcgccatca ttattgccgg
    15361 accgtggatg ctcaatctgt tgctggatta cgtccgcacc ttgttcacta acctgccgta
    15421 tatcatcggg tagcccgtac aatgatgcag gagacaagcg atcaatggct ttcctggtta
    15481 agcctgtact tctggccgtt actgcgcgtg ctggcgctca tttctaccgc gccaattctg
    15541 agcgaacgca gcgtgccgaa acgggtaaaa ctgggtctgg caatgatgat cacgttcgcc
    15601 attgccccat cattacctgc caacgatgtt cctgtttttt cgttctttgc tctgtggctg
    15661 gccgtacagc agatcctgat cggcattgcg cttggtttta ccatgcaatt tgcctttgcc
    15721 gctgtgcgaa ccgctggcga aattatcggt ctgcaaatgg ggctgtcatt tgcgacattt
    15781 gtcgatcctg gcagccatct caatatgccc gttttagcgc gcatcatgga tatgctggca
    15841 ttactgctgt tcctgacatt taacggtcat ttatggttga tttcactgct ggtcgatacc
    15901 tttcacaccc tgccgattgg tagcgaaccg ttgaacagca atgcgtttct ggcacccacc
    15961 aaagcaggga gtttgatttt ccttaacggg ctgatgctgg cgttaccgct cattactctg
    16021 ttgctgaccc tgaatctggc attaggttta ctgaatcgta tggccccgca attatccatt
    16081 tttgttattg gatttccatt aactctgact gtcggcatct ctttaatggc ggcattaatg
    16141 ccgttaattg cgcctttttg cgaacattta ttcagtgaaa tttttaattt gctggctgat
    16201 attattagtg aattaccatt aatataattc cgtaacgttt atcatgctac cctaaggatt
    16261 atccgaaaaa taatatctgc gaacatcttc caggatactc ctgtagcgaa atatttgttt
    16321 taagctcact cacatatcgc aacatttact ttaagacaat tccaggcaaa ttatacaaca
    16381 ctttacggga tagtaagtcc gcctgaaaaa ttgcgagagt ggcgcattag gtgacccatg
    16441 ttgttccgtt tagtcatgat gaaatattca ggtaagggga attatcgtta cgcattgagt
    16501 gagggtatgc catgtcaacg attattatgg atttatgtag ttacacccga ctaggtttaa
    16561 ccgggtatct gttgagtaga ggggttaaaa aaagagaaat caacgacatt gaaaccgttg
    16621 atgaccttgc catagcttgt gattcacagc gtccttcagt ggtgtttatt aatgaggact
    16681 gtttcatcca cgatgcttct aacagtcagc gcatcaagca catcattaat caacatccca
    16741 atacgttatt tatcgttttt atggcaattg ccaatgttca ttttgatgaa tatctattgg
    16801 tcagaaaaaa tttattgatc agttctaaat cgattaaacc ggaatctctc gacgatatcc
    16861 ttggcgatat tctgaaaaaa gagacaacga taacctcgtt tttaaatatg ccgacgttat
    16921 cattgagccg aaccgaatcg agtatgttgc gaatgtggat ggcaggtcag ggaaccattc
    16981 aaatctccga ccaaatgaat atcaaagcca agaccgtttc atcgcataaa ggtaatatta
    17041 aacgtaagat caaaacgcat aataaacagg ttatctacca tgtcgtccga ctgacggata
    17101 atgtgactaa tggtattttt gtcaacatgc gctaacacat tctgactggt ggtttcccac
    17161 cagtcaggct gaataagatt actctgcttt ctcaacaaag ataccgtcct gatgccctgc
    17221 ttcattaaag aaccagatgc cgagcgggta gtcttccagc gcaaccaggt acattgtgcc
    17281 ttcactaaac tcctcaactg ccagtaccac tccaggacga cgcggaccgc catccgtttt
    17341 gactgttacc cgatcattca ccttcatcgt tttcctcctg tggctttgtg ccagtgtaga
    17401 acaatttcgt ttttctggca gcgccaagcg cgcgcgagtg ctgattttct cgacggtcta
    17461 tacttaagag atgccaggcg gacttaacga ctggcggcaa caacagagta acggttgcga
    17521 ggaaagatga tgaaaaccgc aaaagtgtac agcgataccg caaaacgtga ggtcagcgtc
    17581 gatgtcgatg ccctgctggc ggcgatcaat gaaattagcg aaagcgaagt tcatcgcagc
    17641 cagaacgatt ctgaacacgt tagcgtcgat ggacgtgaat atcatacatg gcgtgaattg
    17701 gcggatgcct tcgaactgga tattcatgac ttcagcgtct ctgaagtgaa tcgttgaatg
    17761 cacaataaaa aaatcccgac cctgagggtg tcgggatgaa acttgcttaa gcaagaagca
    17821 cttaaaaaat tcgttacacc aggaaatctg atgtgttcat caccttatct gcaatttttt
    17881 tcgctgacaa gaaaatattc gcgctatgaa tgattaacgc tacttatgaa tagaaatgtg
    17941 acgcgcgtca ccccttggtg gctctggtgt caacgggatc gctcccgaaa aaatcatctt
    18001 tgattatttg tgctgttttg agaatcatcc tggtaagggg agtactgcgc gaccatgttt
    18061 tcaattcaac aaccactact ggtttttagc gatcttgatg gcaccctgct ggacagtcat
    18121 agttatgact ggcaaccggc agccccctgg ctcagccgtt tacacgaagc caatattccc
    18181 gtcattctct gtagcagtaa aactttagcg gaaatgctgt acttgcaaaa aatgttgggg
    18241 ctacaaggct taccgctgat tgcagagaat ggcgcagtga tccagcttgc tgaacaatgg
    18301 caggatatag acggttttcc acgcatcatc tcaggtatta gccatggcga aatctgccag
    18361 gttttaaata cgctacgcga gaaagaacat tttaaattca cgacttttga tgatgtcgac
    18421 gatgcaacca ttgccgaatg gacgggatta agccgtagct aggcggcgct gacgcagctg
    18481 catgaggcgt cggtaacgct aatctggcgc gacagtgacg agcgtatggc acaatttatc
    18541 gctcgtctga acgaactggg cttgcagttt atgcagggtg cgcgcttctg gcacgtactg
    18601 gatgcctctg ccggaaaaga tcaggctgcc aactggatta tcgcgaccta tcaacaattg
    18661 tcaggcagac gcccaaccac acttggcctg ggcgatgggc caaacgatgc gcctttactg
    18721 gaggtaatgg attacgcggt gattgtgaaa gggctaaacc gtgaaggggt acatctgcat
    18781 gatgaggatc cggcccgcgt ctggcgaacg cagcgtgaag gaccggaagg ctggcgtgaa
    18841 gggctggacc attttttctc cgcccgttaa gcgttatcgc tcgcgaatac ccgattgcgc
    18901 ccggcctgtt ttgcgaggta aagccgacgg tcggccaatg actggagttg ttcaaaatca
    18961 taatcaccgg tttcctcgct gctacttacc cccagcgagg cactgatgcg tatcgttgta
    19021 ctcttggcga tcagcatctc tttttcattt aacttcaggc gaatacgttc tgcgacctcc
    19081 gcagcctgcg tcagattcgc gcctggcaga atcacacaaa actcctcacc accgacccgc
    19141 ccggcaacgt cctgcgcacg caaggaactg ctaattaatc cggcagcatg agaaagaaca
    19201 cggtcgcccg cctgatgacc aaagcggtca ttaatcgctt taaaatggtc aagatcgacc
    19261 tgaatgacag aaaaaggatg ttggtgcgtc tgacacaatt tagcgagcgg acgggctttt
    19321 tcgaacagtg cgccacggtt atataaacgc gttaaggtgt cgtgccacgc ctgccactgc
    19381 aacgagcttt gcagaacata catgttgctg accatccggc gaatcacata ccaggagagg
    19441 agtaacatgg tggtaaagag cgcccacagc agggttaatg caatgctgat actgccgaaa
    19501 tcgccgcgca cgccttcgct tagcgtatgg acgcgcgcca gcacaccgtc gaaatggtcc
    19561 agacgttccc agctaacata gcgactgtcc atacgaatgc cgccccgcgt gtcatgttcc
    19621 attgcctgcg ccagcaaggc cagttcacga ggatcaaaaa tattccctgt tggatggtca
    19681 ggattggaag aggtcaaaaa tctcagctta ctgtcataga gctgatactc accatcgagg
    19741 tttttatcga tggcgtttct taaaaattgc tgcatagtac gcacgggaat actcatcccc
    19801 agcacgccat accagtagtt attactgtct accggaacac tgacggtaac ctgcggttca
    19861 gtattgctgg cgtgttccgg ttgcgaagtg aaccagcgta ccgcgcggtg acgattttct
    19921 cgttgcgaat ggccgataaa ccagggttgg gtgacatagc cgtaataacg cgttggtaca
    19981 ttgcgcgtaa acaaggtcgg ctgcgtcgaa acgtaaaatc cagcacgcga gacatacatc
    20041 gcctgttcaa ccatcgacga ggagttgtgc gccagtcgca gcaagtaacc aacttccagt
    20101 gcagcggtaa tttcattgtc gaggctttca ttttcgcgag acaggaaatt cccctcgctg
    20161 actaatgcat ccgatacgcc attgactggc agggtgcgcc gtcggttgag ttcgatttgc
    20221 caggcgtgct cgtcgcgatg ctgttcgaac tcggttacgg cattacgcag agaggtgaaa
    20281 tccagtggcg ccacgagagc ttcgcgcatg ccattacgca aaaagatcag tttgtcgacg
    20341 ttatactgca aatgcttatc gagcgcgttg gctacgtttt ccagatgatt acgctagctg
    20401 gagatatagg catcttccag caccaccact tcacgccagg tgagcaaggt ggaaaaaagc
    20461 aataccacga taaagcagag attaacgaca tgaccgggcc ccaggcggcg tgcgagtttt
    20521 ttcaaccagc tctggttttc catttttgtc tcgtgctgca ccctgacgac tcccaacctg
    20581 cttttttatg attctggcat aacccggcaa tagcgtaccg caaaacagcg ataattttat
    20641 aatggtggca tgataagacg cgactatcat tcattgcagg cattgggaca ccaggccgga
    20701 tgtgatccgg cctggtgtta atgataccct ttcaaacttc ttccgcagaa cgactcccct
    20761 cccccggaac aagatcggtt tcatgaaaag cttcccgctt gaccccgtaa ccgtcatacc
    20821 aacgacactc aaccataccg ctggagtatc cagtgacaat catccgcggg ccgccctctt
    20881 taaccgtaac ttcctcacta accataaagc tcatactcgc ctcctttttt cgagtgaaac
    20941 ttgttcacct tagttgaaga tggcgaattt tgcataatag ccagtgcgag tattaatgtg
    21001 cctgaccgcg cattttcgct actgctttca ctcccagcac cacgatgccg ccgatgataa
    21061 atccaagaat cagatttaaa acagtcggta atatcatcgc caccactgca ctttgctgcc
    21121 cggcgatatg ttcaatagcg tgatgcagcg gcgcaatacc atgtaccaca atcccgccgc
    21181 cgacgaggaa catcgccagc gtgccgacaa tcgataatgc tttcatcagc caaggcgcga
    21241 taatcaataa ccctttacct aatgcctgca tcagcgcgct agatttttcc gccagccaat
    21301 accctaggtc atcaatctta acgataaccc ctaccagacc gtagacgccc acggtcacta
    21361 ccagcgcgat gcctgaaagc accagcacct gattaagcaa cggcgcctct gccacaatcc
    21421 ccagcgtgat ggcgacgatt tccgcagaca aaataaaatc ggtacgaatc gcccctttaa
    21481 ttttgtcctt ttcaaacttc agcggatcct gcgccgccag cttctccaga cgctgctggc
    21541 tctgcgctgg atcttcttta tgtttacgcg cctccagcat atgcagcact ttctctaccc
    21601 cttcaaagca gagaaacgcg ccaccaatca tcaacagtgg cgtaatcgcc cacgggataa
    21661 acgcactgat gatcagcgcc agcggcacca gaatcacttt attgatcagc gatcctttcg
    21721 ccacgcccca gaccacggga agttcccggt tggcccgcac gcctgaaact tgttgcgcat
    21781 tgagcgataa atcatcccct aatacaccgg cggttttctt cgccgccagt ttgcccatca
    21841 cagagatatc gtccagcagt gtggcgatat catcgagcaa cgtcagtaaa ctactcccgg
    21901 ccaaaagcat tccctctcat ttttattgtt gaagggtgta agtatgaagc aaaaacggca
    21961 gctctgatac ttgcttcaca ggtcaacatt tatttaacaa tcaagtgcaa ttatatctct
    22021 cgccagcaga atgattttca cgtttactat gaaccacctt tttatttccg ccgtgaggga
    22081 ttatgcgttt ccgccagttg ttaccgcttt ttggcgcgct gtttgcgttg tatatcattt
    22141 ggggctcaac ctattttgtc attcggattg gcgtgggaag ctggcctccg ttaatgatgg
    22201 cgggcgttcg attccaggca gccggtattt tattgctggc atttttgcta ctgcgcggac
    22261 acaaacttcc cccgctacgt ccgctgctca atgccgcgct gattggcctg ttattgctgg
    22321 ctgtcggtaa tggcatggtg acggttgccg aacatcaaaa tgttccttcc ggcatcgccg
    22381 ccgtagtgat tgcaaccgtg cccctcttta ccctgtgctt cagtcgcctg tttggcatta
    22441 aaacgcgcaa actggaatgg gtgggtattg ccattgggct tgccggaatc atcatgctca
    22501 atagcggtgg aaatttaagc ggcaatccgt ggggcgcgat tctgatttta atcggctcga
    22561 ttagctgggc gtttggctca gtttatggct cgcgcattac cttacctgta gggatgatgg
    22621 cgggtgcgat tgagatgctg gcggcaggcg tggtgttaat gatcgcatcg atgattgcgg
    22681 gtgaaaaact gacggcgctc ccttccctgt caggcttcct tgcggtcggc tatctggcgc
    22741 tgtttggttc gattatcgcc attaacgctt atatgtattt aatccgtaat gtcagtccgg
    22801 ctctcgccac cagctacgcc tacgttaacc cggtggtcgc ggtcttgctg ggtacgggac
    22861 tgggtggcga aacgctgtcg aagattgaat ggctggcgct cggcgtaatt gttttcgcgg
    22921 tggtactggt cacgttggga aaatatctct tcccggccaa acccgttgtc gcgccagtta
    22981 ttcaggatgc atcaagcgag taaatgaatc ccctgcgtgt cgatctgcgc gctggcacct
    23041 tcgccgcaga tccactcttc cagacgctcg gtaagcgccg catccgtcag cttctcgcgc
    23101 ctacgtaacg cacactccca gacaatcagt acgcgccagc cgagctcctg caagcgactg
    23161 atatcgcggc gatcgcgttc aacattttta cctatcttct ccagccagaa ctcagttcga
    23221 gtcgcaggca ctttaaacag atagcagtga tgatgatgcc agaagcagcc gtgggtaaat
    23281 atcacgcagc gatattcatc aacgacaaaa tccgggcgtc cgggcagact ggcgtcctga
    23341 acgcgaaacg caaggccctg cccggttaac aaactggcga ggcgcttctc aatcgccgta
    23401 tcacgcgtgg caatcgcgcg catatttttg ctgcgggtgg ccttatcgtg aacgtcgacc
    23461 atgttgtgcc tcttgctgac gcaacgccac cgcctgtttg atttttggct caagcagttt
    23521 tgccaccgcg gcaaagaccg gcacgaccac cgagttgccg aactggcgat aggcctgagt
    23581 gtccgaaacc ggaatgcgga atttcgcttc tcccggcgct tcaaaaccca ttaagcgcgc
    23641 gcattcacga ggcgttaacc gacgtggacg atgttgctga ttcagcggat cgtcaaagtc
    23701 tttctcaccc gtggccatat cccaaccgcg atcgattaaa atttccgcgc catctttgta
    23761 ataacgagca gacagcgtac gggtgacgct ttgcggattg ttcggataaa ccattccata
    23821 accgaagccg ttaccgcgcg cctgatgttt tttcgcatat cgatagaggt acttccacag
    23881 caccggcgtc aggatatatt tcgcctcaac catcgggtcc agcagctgcg ccagcgtcac
    23941 tcgctgtgca gggaaacatt cgctgatatc acgcagagta aaatcggctt tcagattcag
    24001 atcgcgacga aaacccacca gcacgatgcg ttcacggtgc tgcggcagga aatgtttacc
    24061 atcgatgatt ttcggatcgt ccggcccgtt atcttctgca tcggcaacgt catagcccag
    24121 ttcgtccagc gtctgcatga tgatgcggaa cgtttttccc ttatcgtgac tcttcaggtt
    24181 tttgacgttt tcgagcacaa acatcgccgg acgacgcgcg tcgataatgc gcaccacatc
    24241 aaagaacagc gtgccctggg tatcgcaggc aaaaccgtgc gcccgcccga gcgagttctt
    24301 tttcgatacg ccagccagcg aaaatggctg acaagggaaa ccggccagta aaacatcgtg
    24361 ttcaggaatg tgttgacgaa tatgttccgc cgccgcctca tcactcacgc cttcttgatg
    24421 actgagggtg atgtcgcgga tatcttcatt aaaatgatgc gtcgccgggt cgcaataatg
    24481 gttggcttta taagtgcgta ccgcatgttt gttccattcg ctggtaaaca cgcactgtcc
    24541 accaatcgat tcaaaaccac gacggatgcc gccaattccg gcaaatagat cgataaagcg
    24601 aaacgcataa tgcggatgat gttccggtgg tttgggtaac aacgtttgca gatgggcaaa
    24661 ctctttctca cttaaacggt gccatgccga gtcattcgcc agcgcacgtt ttaaaattgc
    24721 cgcgctccag tgattctcac ccacaccatt aagctgcgcc accagcattt taacatcata
    24781 aatttgcagc agtttctcca gcattgcctg tgcggcattc ccggtgctgt atgaatcggt
    24841 tactgatata ttttcctgca tagatttaac cggccatctg agatggccga caggttaaca
    24901 taattactgc cgtttagggc cacgcgctca gccagcgggt gaaaacgcat ctatcacctt
    24961 gtgatcgata ccttcattct cccccgccag ttcggcaccg agcttcgcca tcaaacagcg
    25021 ccaagctcaa tgcccctgaa acggcaaata cgcgcgccag gccaatcgcc cccagggctt
    25081 ccagccgatc cgcatcctgg acgattttag cttccgttgt taagggggcg atttgcgcgc
    25141 tgaaactgtg agcggcaatg gcatgacaaa cggcctcaat tttctccgcc ggaaattgca
    25201 cgaactcttc acggagcagg cgacgcgtct cttctgccgc caggattgaa gaacgctgcc
    25261 gttgcggatg atttttcgcc aggctcacga tatcgtgaaa ataacaggcg gttaaaatca
    25321 ccagcatatc gacatcgtca tctgccgcca gtttctgtgc cgtagcccag acacggcgaa
    25381 aatgacacag gtcatgcgcc gcgtcctgat gttgatgatg gtttttcaac cagttctcga
    25441 actatgcctg ccagtgttgt aactccatac gcgctccgtt gccaaagagt ctcaacctta
    25501 gcagttttta ttcatagcgt ctgcaacgtg cgggacgagg tcgggtgtac catgcaatgc
    25561 cgcctaacaa ggcaccaacc agggccataa acagaaatcc gaccaatgct cctaaccact
    25621 tccccgcggt agatcctaaa tcactttcaa atcccgacac gggggcatcg gggatccggc
    25681 tcatcaccca gatgtatcgc gccattgccc agacgaaata gccacaccat gtatagaaag
    25741 ccc
//
LOCUS       DAJTSF010000056        25661 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000056 DAJTSF010000000
VERSION     DAJTSF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.056"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="OO335_002399"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="OO335_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6002751.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="OO335_002400"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="OO335_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6002752.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="OO335_002401"
     CDS             2630..3391
                     /locus_tag="OO335_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6002753.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="OO335_002402"
     CDS             complement(3442..4326)
                     /locus_tag="OO335_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6002754.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="OO335_002403"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="OO335_002403"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6002755.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="OO335_002404"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="OO335_002404"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6002756.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="OO335_002405"
     CDS             6202..6474
                     /locus_tag="OO335_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6002757.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="OO335_002406"
     CDS             6844..7920
                     /locus_tag="OO335_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6002758.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="OO335_002407"
     CDS             7947..9326
                     /locus_tag="OO335_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6002759.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="OO335_002408"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="OO335_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="OO335_002409"
     CDS             10409..11245
                     /locus_tag="OO335_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR6002760.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="OO335_002410"
     CDS             11250..12197
                     /locus_tag="OO335_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR6002761.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="OO335_002411"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="OO335_002411"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR6002762.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="OO335_002412"
     CDS             13324..14082
                     /locus_tag="OO335_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6002763.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="OO335_002413"
     CDS             14200..15558
                     /locus_tag="OO335_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6002764.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLC
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="OO335_002414"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="OO335_002414"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6002765.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="OO335_002415"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="OO335_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6002766.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="OO335_002416"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="OO335_002416"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6002767.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="OO335_002417"
     CDS             19503..20054
                     /locus_tag="OO335_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6002768.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="OO335_002418"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="OO335_002418"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6002769.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="OO335_002419"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="OO335_002419"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6002770.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="OO335_002420"
     CDS             23307..24347
                     /locus_tag="OO335_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6002771.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..>25661)
                     /gene="gdhA"
                     /locus_tag="OO335_002421"
     CDS             complement(24464..>25661)
                     /gene="gdhA"
                     /locus_tag="OO335_002421"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR6002772.1"
                     /translation="LLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKG
                     GMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLM
                     TELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPE
                     ATGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGT
                     VVDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQN
                     ELDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLE
                     MAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAM
                     LAQGVI"
BASE COUNT         6519 a         5970 c         6624 g         6548 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacaat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt tgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc ggcgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaag ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatatgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgccg ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagtaa t
//
LOCUS       DAJTSF010000057        24890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000057 DAJTSF010000000
VERSION     DAJTSF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.057"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..795
                     /locus_tag="OO335_002422"
     CDS             <1..795
                     /locus_tag="OO335_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6002773.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="OO335_002423"
     CDS             806..1897
                     /locus_tag="OO335_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6002774.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="OO335_002424"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="OO335_002424"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6002775.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="OO335_002425"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="OO335_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6002776.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="OO335_002426"
     CDS             4859..5254
                     /locus_tag="OO335_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6002777.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="OO335_002427"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="OO335_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6002778.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="yraP"
                     /locus_tag="OO335_002428"
     CDS             5874..6449
                     /gene="yraP"
                     /locus_tag="OO335_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6002779.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="OO335_002429"
     CDS             complement(6563..7603)
                     /locus_tag="OO335_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6002780.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="OO335_002430"
     CDS             complement(7676..8311)
                     /locus_tag="OO335_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6002781.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="OO335_002431"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="OO335_002431"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6002782.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="OO335_002432"
     CDS             complement(8937..9380)
                     /locus_tag="OO335_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6002783.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="OO335_002433"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="OO335_002433"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6002784.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="OO335_002434"
     CDS             complement(9720..10223)
                     /locus_tag="OO335_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6002785.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="OO335_002435"
     CDS             complement(10217..10741)
                     /locus_tag="OO335_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6002786.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="OO335_002436"
     CDS             10950..11945
                     /locus_tag="OO335_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6002787.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="OO335_002437"
     CDS             11954..12832
                     /locus_tag="OO335_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6002788.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="OO335_002438"
     CDS             12913..13920
                     /locus_tag="OO335_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6002789.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="OO335_002439"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="OO335_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6002790.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="OO335_002440"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="OO335_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6002791.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="OO335_002441"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="OO335_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6002792.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="OO335_002442"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="OO335_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6002793.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="OO335_002443"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="OO335_002443"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6002794.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="OO335_002444"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="OO335_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6002795.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="OO335_002445"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="OO335_002445"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6002796.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="OO335_002446"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="OO335_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6002797.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..>24890)
                     /gene="infB"
                     /locus_tag="OO335_002447"
     CDS             complement(22807..>24890)
                     /gene="infB"
                     /locus_tag="OO335_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6002798.1"
                     /translation="EARRVAEEARRMAEENKWTDNAEPTEDSSDYHVTTSQHARQAED
                     ESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRGGKGGKRKGSSLQQGF
                     QKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAMATINQVIDQETAQLV
                     AEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHVDHGKTSLLDYIRSTK
                     VASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARGAQATDIVVLVVAADD
                     GVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQYGILPEEWGGESQFV
                     HVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFLDKGRGPVATVLVREG
                     TLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLSGVPAAGDEVTVVRDE
                     KKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVNIVLKADVQGSVEAIS
                     DSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNVRADASARKVIEAESL
                     DLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKSPKFGAIAGCMVTEGV
                     VKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIGVKNYNDVRTGDVIEV
                     FEIIEIQRTIA"
BASE COUNT         5999 a         6867 c         6310 g         5714 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt
//
LOCUS       DAJTSF010000058        24655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000058 DAJTSF010000000
VERSION     DAJTSF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.058"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..386)
                     /locus_tag="OO335_002448"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="OO335_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="OO335_002449"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="OO335_002449"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6002799.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWAHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="OO335_002450"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="OO335_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6002800.1"
                     /translation="MKGGKRVQTARPNRINGEIRAPEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="OO335_002451"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="OO335_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6002801.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="OO335_002452"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="OO335_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6002802.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="OO335_002453"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="OO335_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6002803.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="OO335_002454"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="OO335_002454"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6002804.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="OO335_002455"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="OO335_002455"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6002805.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="OO335_002456"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="OO335_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6002806.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="OO335_002457"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="OO335_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6002807.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="OO335_002458"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="OO335_002458"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6002808.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="OO335_002459"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="OO335_002459"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6002809.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="OO335_002460"
     CDS             10748..11212
                     /locus_tag="OO335_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6002810.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="OO335_002461"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="OO335_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR6002811.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /locus_tag="OO335_002462"
     CDS             12236..13672
                     /locus_tag="OO335_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6002812.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO335_002463"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO335_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6002813.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO335_002464"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO335_002464"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6002814.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO335_002465"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO335_002465"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6002815.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMYNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="OO335_002466"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="OO335_002466"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6002816.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO335_002467"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO335_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR6002817.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="OO335_002468"
     CDS             complement(20505..20798)
                     /locus_tag="OO335_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6002818.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="OO335_002469"
     CDS             complement(20795..22084)
                     /locus_tag="OO335_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6002819.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNPPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="OO335_002470"
     CDS             complement(22140..23078)
                     /locus_tag="OO335_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR6002820.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="OO335_002471"
     CDS             complement(23098..23862)
                     /locus_tag="OO335_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR6002821.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24183..>24655
                     /locus_tag="OO335_002472"
     CDS             24183..>24655
                     /locus_tag="OO335_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR6002822.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT         6103 a         5949 c         6488 g         6115 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg gcgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgccccggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat acatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggcgggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
    23941 gcgggggggg gggggcgacg gagcaagctg ttttccttgc caacaatctt gcaaaaaaaa
    24001 tgcgattgct aaaacaaaag tgcaatttgc ggcaatatag ttattccatg accgtacaat
    24061 cttgcattca gtgtaaataa tcttgtttag ctctcgctaa acgcggttgt ataaccgcaa
    24121 tacacgccgt attttatccg gcatatattg tttttgcttg ctcatggaat agggaggttg
    24181 tcatgtatca acgctgtttt gataatgcca gtgaaacgct gtttgtcgcc ggtaaaacgc
    24241 cacggctttc acgttttgca tttagcgatg atccaaaatg ggagtctgga catcacgttc
    24301 atgacaatga aaccgagctg atttacgtca agaaaggggt tgcaaggttt accatcgatt
    24361 cttcgttata tgtcgcacat gcggatgaca ttgtggtgat agaacgcggc aggctgcatg
    24421 cggtggcctc tgacgttaac gatccggcaa cgacgtgtac ctgtgcgctg tacggctttc
    24481 agtttcaggg ggctgaggaa aatcagttac tgcaaccgca ttcttgtccg gtaattgccg
    24541 cggggcaggg aaaagaagtc ataaaaacct tatttaatga gctaagtgtg attttgccgc
    24601 aaagtaaaaa tagccaaaca tcttcgttat gggatgcatt agcctataca ttagc
//
LOCUS       DAJTSF010000059        24586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000059 DAJTSF010000000
VERSION     DAJTSF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.059"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(2..94)
                     /locus_tag="OO335_002473"
     tRNA            complement(2..94)
                     /locus_tag="OO335_002473"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO335_002474"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO335_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6002823.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO335_002475"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO335_002475"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6002824.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO335_002476"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO335_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6002825.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO335_002477"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO335_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6002826.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO335_002478"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO335_002478"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6002827.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO335_002479"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO335_002479"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6002828.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO335_002480"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO335_002480"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO335_002481"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO335_002481"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6002829.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO335_002482"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO335_002482"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6002830.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO335_002483"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO335_002483"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR6002831.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO335_002484"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO335_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR6002832.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO335_002485"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO335_002485"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6002833.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO335_002486"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO335_002486"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6002834.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO335_002487"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO335_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6002835.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO335_002488"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO335_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR6002836.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO335_002489"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO335_002489"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR6002837.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO335_002490"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO335_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6002838.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO335_002491"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO335_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6002839.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO335_002492"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO335_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6002840.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO335_002493"
     CDS             complement(20900..21019)
                     /locus_tag="OO335_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR6002841.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO335_002494"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO335_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6002842.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO335_002495"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO335_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6002843.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..>24586)
                     /locus_tag="OO335_002496"
     CDS             complement(23682..>24586)
                     /locus_tag="OO335_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6002844.1"
                     /translation="GIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTGH
                     RDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVAR
                     LLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAIC
                     CVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDRL
                     PSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGS
                     ALLFRRMALVELAVLVVIIVSRGA"
BASE COUNT         5799 a         6918 c         6306 g         5563 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat atgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccg
//
LOCUS       DAJTSF010000060        24454 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000060 DAJTSF010000000
VERSION     DAJTSF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.060"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..202)
                     /locus_tag="OO335_002497"
     CDS             complement(<1..202)
                     /locus_tag="OO335_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6002845.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLAPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGM"
     gene            complement(295..867)
                     /locus_tag="OO335_002498"
     CDS             complement(295..867)
                     /locus_tag="OO335_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR6002846.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            1085..1945
                     /gene="tesB"
                     /locus_tag="OO335_002499"
     CDS             1085..1945
                     /gene="tesB"
                     /locus_tag="OO335_002499"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6002847.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1994..3280)
                     /gene="amtB"
                     /locus_tag="OO335_002500"
     CDS             complement(1994..3280)
                     /gene="amtB"
                     /locus_tag="OO335_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6002848.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3310..3648)
                     /gene="glnK"
                     /locus_tag="OO335_002501"
     CDS             complement(3310..3648)
                     /gene="glnK"
                     /locus_tag="OO335_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6002849.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3829..5610)
                     /locus_tag="OO335_002502"
     CDS             complement(3829..5610)
                     /locus_tag="OO335_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6002850.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5603..7375)
                     /locus_tag="OO335_002503"
     CDS             complement(5603..7375)
                     /locus_tag="OO335_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6002851.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7405..7863)
                     /gene="decR"
                     /locus_tag="OO335_002504"
     CDS             complement(7405..7863)
                     /gene="decR"
                     /locus_tag="OO335_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR6002852.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(8017..8835)
                     /gene="cof"
                     /locus_tag="OO335_002505"
     CDS             complement(8017..8835)
                     /gene="cof"
                     /locus_tag="OO335_002505"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR6002853.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8935..10627
                     /locus_tag="OO335_002506"
                     /pseudo
     CDS             8935..10627
                     /locus_tag="OO335_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10692..11387
                     /gene="queC"
                     /locus_tag="OO335_002507"
     CDS             10692..11387
                     /gene="queC"
                     /locus_tag="OO335_002507"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6002854.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11439..11837)
                     /gene="fadM"
                     /locus_tag="OO335_002508"
     CDS             complement(11439..11837)
                     /gene="fadM"
                     /locus_tag="OO335_002508"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6002855.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11931..12302)
                     /locus_tag="OO335_002509"
     CDS             complement(11931..12302)
                     /locus_tag="OO335_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6002856.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12453..14324)
                     /gene="ppiD"
                     /locus_tag="OO335_002510"
     CDS             complement(12453..14324)
                     /gene="ppiD"
                     /locus_tag="OO335_002510"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6002857.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14516..14788)
                     /gene="hupB"
                     /locus_tag="OO335_002511"
     CDS             complement(14516..14788)
                     /gene="hupB"
                     /locus_tag="OO335_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6002858.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14997..17351)
                     /gene="lon"
                     /locus_tag="OO335_002512"
     CDS             complement(14997..17351)
                     /gene="lon"
                     /locus_tag="OO335_002512"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6002859.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17539..18813)
                     /gene="clpX"
                     /locus_tag="OO335_002513"
     CDS             complement(17539..18813)
                     /gene="clpX"
                     /locus_tag="OO335_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6002860.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILSICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18939..19562)
                     /gene="clpP"
                     /locus_tag="OO335_002514"
     CDS             complement(18939..19562)
                     /gene="clpP"
                     /locus_tag="OO335_002514"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6002861.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19809..21107)
                     /gene="tig"
                     /locus_tag="OO335_002515"
     CDS             complement(19809..21107)
                     /gene="tig"
                     /locus_tag="OO335_002515"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6002862.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21451..21768)
                     /gene="bolA"
                     /locus_tag="OO335_002516"
     CDS             complement(21451..21768)
                     /gene="bolA"
                     /locus_tag="OO335_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6002863.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            22073..22651
                     /locus_tag="OO335_002517"
     CDS             22073..22651
                     /locus_tag="OO335_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6002864.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22695..24170
                     /gene="ampG"
                     /locus_tag="OO335_002518"
     CDS             22695..24170
                     /gene="ampG"
                     /locus_tag="OO335_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6002865.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5745 a         6731 c         5900 g         6078 t
ORIGIN      
        1 ccatgccaac aatacaccag accatcattt cgaggggaag acggcgcttg cgtagcgtta
       61 cagtacctga ttcggcaagg caacgagaga tgagttcggg ggcgaggtaa tcccccagag
      121 aagtcagtgg gttacgcaga gaatcgtaac gggataccag atcaagagcc tgtccaatgt
      181 gcataaaaaa atccggaaac aagtgagcgt ttccggattc ttacacagcc actggatcgg
      241 tcaactgatc cttaactgat cggcattact ccgggcggag agggttaacg tcgcttaata
      301 ttgcgtagga gcgggaactg ccgaagacgg attcacctga gttggtgaag tcgaaggtac
      361 ggtagtcgtt gcgccaccgc tggcctgaac cggcacggcg gtttgctgta ccggcaccaa
      421 tgtcaggtcg gctttagttc cgccctggtt gatcaccggc tgaacggtat cggtgataaa
      481 taccagtttg tcattcacgg taatcgccgc actcaacaga atacgcgcgt tcggctgaac
      541 atctgccggg ttaaatggca gaacaaagct gaatggtgac tgtttacctt cagtacgcac
      601 cgctttctgc gccagcactt ttgacggtgc atcggctaac gacgcgtcag aaagtgtcac
      661 ggtcagcaca gcatcaggcg gcagtgcgac tttctgacgg atccagacag taccggagac
      721 attcggttgc tggatagcag gttgttgtgt tgctgaaata gacgtatttg cagccggggc
      781 tggcgtctga atatccgcgc ttttatctgc gcaagccgcc aacgcaatcg caaccgctaa
      841 accactggcc atgtgcacga gtttcattca tttctcctta ttatcaatgc accagcgggc
      901 taactttcct cgccggaaga gtggttaaca aaatagtaac gtcaacaagt gtggcacaca
      961 tcacgcattt ctgcctgtaa ttagcccgta attcagacta tttcacccat cggaccagtg
     1021 gcaaatttgc gttatactca actcactttg gcttgctgcg gcagctttgt tactggagag
     1081 ttatatgagt caggcgctaa aaaatttact gacattgtta aatctggaaa aaattgagga
     1141 aggactcttt cgcggccaga gtgaagattt aggtttacgc caggtgtttg gcggccaggt
     1201 cgtgggtcag gccttgtatg ctgcaaaaga gaccgtcccg gaagagcggc tggtacattc
     1261 gtttcacagc tactttcttc gcccaggcga tagtaagaaa ccgattattt atgatgtcga
     1321 aacgctgcgt gacggtaaca gcttcagcgc ccgccgggtt gctgctattc aaaacggcaa
     1381 accgattttt tatatgacgg cctctttcca ggcaccagaa gcgggtttcg aacatcaaaa
     1441 aacaatgccg tccgcgccag cgcctgatgg cctgccttcg gaaacgcaaa tcgcccaatc
     1501 gctggcgcac ctgctgccgc cagtgcttaa agataaattc atctgcgatc gtccgctgga
     1561 agtccgtccg gtggagtttc ataacccact gaaaggtcac gtcgcagaac cacatcgtca
     1621 ggtgtggatc cgcgcaaatg gtagcgtgcc ggatgacctg cgcgttcatc agtatctgct
     1681 cggttacgct tctgatctta acttcctgcc ggtagctcta cagccgcact gcatcggttt
     1741 tctcgaaccg gggattcaga ttgccaccat tgaccattcc atgtggttcc atcgcccgtt
     1801 taatttgaat gaatggctgc tgtatagcgt ggagagcacc tcggcgtcca gcgcacgcgg
     1861 ctttgtgcgc ggtgagtttt atacccaaga cggcgtgctg gttgcctcga ccgttcagga
     1921 aggggtaatg cgtaatcaca attaaaaaaa tagccggagg tgaaaaccgt ccggctattt
     1981 tttgcagtgc ttgttacgcg ttataggcat tctcgccgtg gctgttgacg tccagccctt
     2041 ctcgctcttg ttcttccggc acacgtaggc caaccgtcag atccgccagt ttgtagccaa
     2101 taaatgccac cacaccggac cagacgatcg taatggcgat gctttccaac tgtaccagca
     2161 actgatggcc catcgtcaca ccttcagcga agcccacgcc gcccagagaa ctggcggcaa
     2221 aaatcccggt catgatacag ccgacaatgc cacaaacgcc gtgcacaccg aagacatcgc
     2281 agggatcatc cacccgcagc aagcgtttga gcatggtaac gccccacaaa cccgccagac
     2341 cagctaccac gccgataatc aacgcgccgc caaccccaat gtagccgcac gctggcgtca
     2401 cgccgaccag accggcaatc gcgccagaac acgctcccag cagagaaggc ttaccacgca
     2461 gcgcccattc accgaagatc cagccaagaa ttgccgccgc cgttgcgacc acagtattca
     2521 caaatgccag tgccgcgatt tcattcgccg tgcccactga cccggcgtta aagccaaacc
     2581 aaccgatata gagaatggca gtcccggtga agaccatcgg caggttgtgc ggtttaaacg
     2641 cctctttacc gaagcctacg cgtttgccta tcagatacgc gcccaccaga ccggcgattg
     2701 ctgcgttaat gtgcaccacc gtgccacccg cgaaatccag cgcaccgtga gaagccagca
     2761 aaccaccgcc ccacaccata tgcgcaatcg gaatgtaaga gagcgtcagc cataccacca
     2821 cgaaaatcaa cacagctgag aagcggattc gttccgccag cgccccaact atcaagccta
     2881 cggtaatgca ggcaaacgat ccctgaaacg ctacgtggat atactgataa atgctgccca
     2941 tcaccgccgt cagttcgatg ttttttagca tcaaccagtt aatgttgccg aagaagttgt
     3001 tgccctcacc aaacgccagc gaataaccgt aaaccaccca gagaatacag accagtgcaa
     3061 acgtcaccgt cacctgcgtc agcatcgaca gcacgttttt gccgcgaatc aacccaccgt
     3121 aaaacagggc aatccccgga atagtcataa acagcaccag cgcagtacaa atcatcataa
     3181 acgcattgtc ggctttatcg gccaccgcag gtgcagccat taccagtccc ggcagcatcg
     3241 ccagtgaagc aagcccagtt tttatcgtcg ctatcttcat ttttcgttcc tgttgctgtg
     3301 tgccagagat tacagcgccg cttcgtcggc ttcgccggta cgaatacgaa tgacgcgttg
     3361 caattcagcg acgaagattt tgccgtcgcc aatttttccg gtgtaagccg ccttactgac
     3421 gatatcgatc acttcatcga gttggtcatc agcaatcgcc acatcaattt ttacttttgg
     3481 caggaagttg acgctgtatt ccgccccccg gtacagctcg gcatgccctt tctgacgccc
     3541 gaaacctttc acttcggtga cggtcaggcc ctgaatacca atggaagata acgcttcacg
     3601 aacgtcttcc agcttgaatg gtttgattat cacggtcacc agcttcatag atcccctccg
     3661 gtcagaattc ggtaatggtt ctgctacacg aagaaggtat tgcaagcggt gtgccagaaa
     3721 tgaaaaacga gaaagagagc aggaagttaa gaagattgaa aatgaaaacg cactatgaca
     3781 gtgcacagtg cgttacattt ttgcaccaac gatgaaatat tgcgctattc aggcgctcaa
     3841 tgattcctct tcacgcacgc tggctgccag ctcttcgccc gcaagttgca gttgatacat
     3901 ctgccagtag cgtccctggg ccgccagcag ttgctggtga gtgccctgct ccacggcttg
     3961 cccacgatga agcaccagaa tggtgtcggc atcaataata gttgataagc ggtgagcaat
     4021 caccaccagc gtggtatgtt cacgcaccgc cgccagagca tgttgaatcg cctgttcagt
     4081 accggagtca atgctggcgg ttgcctcatc aaggatcagg atttgcggcg tctccaccag
     4141 cacgcgcgcc agtgccagca gttgcttttg cccgactgag agattatttc cctgctcgcc
     4201 cagcggcgta taaatacaat cactcatgct gcgcgccagc tccgccagtt gcacggtttc
     4261 cagcgcctgc cagacgcgtt cttcggagat atcccgcccc agcgtcacat tagcgaggaa
     4321 ggtatccgcc agcaccaccg gatcttgctg caccattgcc acgccctggc gcagcgcgct
     4381 atgacttagc gaacttaatg gacgaccatc aaggtgaatc tcaccttccg ttagcgggta
     4441 atagcccatc aataaactgg cgagggtact tttaccactg ccggtatgcc cgaccagcgc
     4501 cacaaagtta cgcgaaggca cagagagatt aatgttcttt agcaccagat tgtcatcgcg
     4561 ataagcaaat gacacgttat cgacttcgat ggtgccactc tgtaacgggt aatcatcatt
     4621 gccatattgc tggcgcggtc cgtccatcag ttcaaacacg cgctcaccag caacaacagc
     4681 ctgttgcagc atcgcctgtt gcgtggtcag ttcgattaag ggttcgttaa gtcgcccaag
     4741 atagctgata aacgcataca acacgcccac ttcaatggtg ccgctggcgg agaagccaaa
     4801 cagcatcaat aagccacaaa gaatgagcga tgaaaacaga ctcagcagcg gacgcagcag
     4861 aaaaccgtcg aggcgcaggg tttgcatcct agccatatag tgtgaacgac tggcctcccc
     4921 catacgttcg ccaaatcgcg cctgctgacg aaactgctgg ataacgctca tgccattgat
     4981 gatttcgtta aagccgtcgt tgatatccgc cagataggcg cgcacacggc ggacaatcgg
     5041 cgtgctgtaa cgctggtata tcaccattac caccagcacc accgggaaaa tcattatcgc
     5101 caccagcgcc attcgccagt cgaggctgaa catcgccacc agcatcgcgc ccaccagtgc
     5161 ggcactgcgc aggacagttg ccactacggt aacgtagaga tcgcggatca cttcagtgtc
     5221 attagtgacg cgggaaatca cctgcccgac gggttgggta tcaaactcgc ttaatggctg
     5281 gcgtaacgca gcatccatca cgtcggtacg caactgttgc actgcgccta ctgccgcccg
     5341 attaaacagc agcgactgcg cgtaatgtag cccggcggca aacagttgta gcccaacata
     5401 cgccgcagcc agccctgcaa ccactttcaa cggcaggtta tttttcgcta ccatattgtc
     5461 gataaaatag ctgataagca gcggcccact gacttctgcc gccgccgcaa cccacatcat
     5521 caggaccgca atccccagcg gtttacgcca cggcgaacca tacgctaaca ggcgcttgag
     5581 agtcggccac agttggctaa aactacgcat cgacggcctc ctcgcgaatt tccggaacgt
     5641 cgtcgagcgc cgcctccagt tgttgatagc gatacatatc gcgataccaa ccgctttgtt
     5701 gcgccagctc atcatgattg ccacgctggg cgatatgtcc gtgctgcatc acaataattt
     5761 cactggcttc cgtcagtgcc gaaaggcgat gggcactgat gattaccgtt cttccctgtc
     5821 cccactgacg caggttatgc aggatctggt gctctgtgcg tccgtccacc gccgaaagcg
     5881 catcatcaag gatgaggatt tccgcgttga ctaataacgc tcgagcaatg gagatacgct
     5941 gtttttgccc accggaaagc atcacgccgc gctcgcccac ttctgtatcg taaccttgcg
     6001 gtagacgcaa aatatcgtca tgtacgctgg ctaaccgcgc gacatgctca atctcttgct
     6061 gggtggcatt cgggcaaccc agcgcgatgt tattcgccac ggtgtcagaa aaaaggaatg
     6121 gcgtctggct gaccaccgcc aggcggctac gccagctatc gagttgtaac ttcgtcagag
     6181 gaatatcatg aaagcgaata tccccctcgc tgacgtcgaa atgacgctga atgagcgaca
     6241 acagggtact tttgccggaa ccagtcggcc cgcaaatacc cagcatctga ccgggtttca
     6301 gggcgaaatt gacgttttcc agcgcaggat ggtcagtctg cggatacgtg aactggtgaa
     6361 tatttacatc cagttcgcca cgcccttccg gcacgggttc gctaccatcg ttcaccaccg
     6421 gcgcttccgc cagcatggcg cgaatacggc tgtatgcagc actaccacgt tccacaatgt
     6481 taaacatcca tgccagcgcc agcattggcc aaatcatcag acctaaatac atcataaaac
     6541 tggtgagctg gcccagcgtt aaactgccct gcaccaccat ccaactaccg ccgccaatcg
     6601 ccagcaagtt cgccatacca atcgcgatat agatggtcgg gtcgaagcga gcatcaatac
     6661 gcgccacccg catgtttttt ttgccggtat cttcggcatc cgcggcaaat aacgccgact
     6721 ggcgatcttc cagaccaaag gctttgatca tgcggatact ggtgaggctt tcctgggtgc
     6781 ggtcattaag actggaaaac gccgcctgtg ccagcttaaa gcgttcatgc aaggcatcgc
     6841 cgttgcgctt aatcatgatc gccatcactg gcatcggtaa cagggcaaat aaggtcaact
     6901 gccagctaat ttgcgtagac atcataatca acacagcgca gcccattacc agtgaatcca
     6961 ccagcgtcag caccccttct ccggcggcaa acacgacgcg atcgacgtca ttggtcgcac
     7021 gagccatgag atcacccgtg cgatgacgca ggtaaaactc cgggtgctgc cggcttagct
     7081 gacggtaata atcttcacgc agttcaacag ccagttggta agacgcgcca aacagcaata
     7141 cgcgccagac ataacgcagg agataaacca caacggcaat cagcaccatg gtggcgatcc
     7201 acatcaggct cagcccggta gtaaagtgtt gttctgtcac gccatcgaca acaataccaa
     7261 ccacttttgg cggaaccagt tgcagcatcg cgataatgac aagcaaggcg acagccccaa
     7321 gatagcgacg ccattcccga cggaaatacc agcttaattg agcaaataat cgcacgcgtt
     7381 gtgtcctgac ctgattctgg atatttattc gatgggtaaa gaagtggtgt atttaatctg
     7441 ttccatcgcg aagctggaag tgacgtccga cagccccggc acgctgttta ccagacgctt
     7501 ataaaactcg tcgtagcgtt tcatgtcggc aacctggacg cgcatcagat aatcgtattc
     7561 accagccatg cgccagaccc ccagcacttc tggcatttcg gtaaccaccg tgacaaagcg
     7621 gcaataccat tcgctgctgt gatgttgcgt ttttatcagc acaaaggcgg tcaggccgag
     7681 gcctattttt tccggatcca gcagggcgac tttgccgata aggataccgt cgtcctccag
     7741 ccgtttcagg cgcttccagc agggggtggt tgtcagatta acggcttcag ccagtgcctg
     7801 caaagagagg gtgcaatcct gctgcagtaa ggccagcagc ttacggtcaa ttttatctaa
     7861 catagccctt ccacggagaa tttttttctc ttttaattct attttaaagg tgaaatcgcg
     7921 acaatttatt ctgtgcggtt taaacaccac ggcacaaaat gacaaattca acgcaaaaaa
     7981 aaaccgggca attgcccggt gctggaaggg atctcgttat tcgggggaat aaggtagatg
     8041 tggatagtcc agccagtgcg tcaaatagtg agagacagcc tgatttcggc aatgtccaat
     8101 caccggtaaa tgcgggagct ccgcgcgcag ttgcggcatc gcattgccca taataaaccc
     8161 gctaccgacg ctgcctaaca tttcgcgatc gttcatcgca tcaccaaagg ccatgcaatc
     8221 gcgcaacgat aaacctaaat gttgggtccg caccgtcaat gcagcgcctt tattgcagcc
     8281 caccggcagc acttcgaggc aatccgtggc ggaaaaacac aaatgtgcac gctcgcctaa
     8341 tgcttcgtat agctggatct gcaagcgtgt aagatcgtcg tgatcgccac agaagcagat
     8401 cttggtgacg ctgccgagtg gcattttttt gacatcgatt atctgataac gaaaaccgct
     8461 atagacaaat acctgcaaca acgcagggct ctctttcccg gtaaaccaac cgtcgtcatt
     8521 gaagatatgc atgctggctc gggtatccca ttgctgatac agcaccagct ccgcgacatc
     8581 cgcaggtaaa tcatcacgat gtaaaagttc accttccaga gaatgcacgc gcgttccgtt
     8641 gccggtaatc aaatatgcat ccagcgatag cgccccgaga atatgctgca tctccagcgc
     8701 atgacgcccc gtggcaaaag tgagggtaat gtcgcgttca cgcagtcgcg ccaaagtaga
     8761 gagggttttc tcacctaaat gatggtcagg catcaataaa gtgccatcca tatcaaatgc
     8821 taccagacga gccatttctt tctccactct gtgacacggt taatggtgat tgagtatcac
     8881 ctgatatata cggaagtaat agtgaatagt taaataatat tgttccgggt ttatatgcga
     8941 ttgctcaacc gtcttaacca gtatcaacgt ctgtggcaac cttcagccgc aaaccgtcac
     9001 cgtcagcgaa ctggccgaac gctgtttttg cagcgaacgc catgttcgta cgctgttgcg
     9061 tcaggcacag gaggcgggat ggctggagtg gcaggcgcag tcaggacgcg gaaagcgcgg
     9121 acaattacgc tttctggtca cgccggaatc gctacgcaat gcgatgatgg aacaggcact
     9181 ggaaaccgga aagcagcaag atgtgctgga gctggcgcaa ctggccccag gtgagctgcg
     9241 cactctgtta cagccattta tgggcggaca atggcaaaac gatacaccca cgttgcgtat
     9301 tccctactat cgcccgctcg aaccgctaca accaggcttt ttgcccggcc gtgccgagca
     9361 gcatctcgcc gggcagatat tttccggcct gacccgcttc gataataata ctcagcgccc
     9421 gattggcgat ttagcgcatc actgggaaac ctctactgac aggttacgct gggactttta
     9481 tcttcgttca accctacact ggcataacgg cgatgcagta aaagcctcac acttacacca
     9541 gcgattattg atgctgttac aactgccagc actggatcaa ttatttatta gcgtgaagcg
     9601 tattgaagtc acccatccgc agtgtctgac cttcttttta catcgccccg attactggct
     9661 tgcgcaccgg ctggcgagct attgcagcca tctggcgcat ccgcaattcc cactgatcgg
     9721 cactggtcct tttcgcttaa cacaattcac agcggagctg gtgcgcctgg agagccatga
     9781 ttattaccat ttgcgtcatc cgctgcttaa agcggttgag tactggataa ctccgccgct
     9841 tttcgaaaaa gatttgggaa ccagttgtcg gcatcccgtg caaatcacca tcggcaaacc
     9901 ggaggagctg caacgggtca gccaagtcag tagtggcatc agtttaggtt tttgctattt
     9961 aacgttgcgc aaaagtcccc gactctccct ctggcaggcg cgaaaagtga tctccattat
    10021 tcatcaatcc ggtttattac aaacgttaga agtcggagaa aacctgatca ccgccagtca
    10081 tgcattactg ccaggctgga ctattccgca ctggcaagta ccggatgaag tcaaactacc
    10141 gaaaaccttg acgctggttt atcacctacc gatagaactt cataccatgg cagaacgcct
    10201 acaggcgaca ctggcagcgg aaggctgtga actcacaatt atttttcata acgcaaaaaa
    10261 ctgggacgac acgaccctac tggcacacgc agacctcatg atgggcgaca gattaattgg
    10321 cgaagcaccg gaatatactc tggagcaatg gctacgttgc gatccactat ggccacatgt
    10381 tttcgacgct ccagcatatg cacatctgca atcgacgctg gacgcggtgc aagtaatgcc
    10441 tgatgaggaa aaccgattta atgccctgaa agcggttttt agccagttaa tggcagacgc
    10501 gacgctgacg ccgctgttca actatcacta tcgcattagt gcccctcccg gcgtgaacgg
    10561 tgtgcgactg acaccgcgcg gctggtttga atttaccgaa gcctggcttc ccgcgccgtc
    10621 gcaatgaata gctggtccgg gttaatcgca ggcgttacca taacgctttt attgttcaag
    10681 caggattatc tatgaaacgt gctgtcgttg tgttcagtgg aggccaggat tccaccacct
    10741 gtctggtgca ggcattacaa caatatgatg aagtccattg cgtgacgttc gattacggtc
    10801 agcgacatcg cgcagaaatc gacgtggcac gcgaactggc gctgaaactg ggagcacgcg
    10861 cgcataaggt tcttgatgta acgctgctca acgagctggc ggtcagcagc ctgacacgtg
    10921 acagcattcc ggtgcctgat tatgaacctg aagccgatgg catcccgaat acgtttgtcc
    10981 cagggcgtaa tattttgttc ctgacgctgg cggcaatata tgcgtatcag gtaaaagcag
    11041 aagccgtaat taccggcgtc tgtgaaacgg atttctccgg ctacccggat tgccgcgatg
    11101 agtttgtgaa agcactaaac catgccgtca gtttgggcat ggcgaaagat attcgttttg
    11161 aaacgccgct gatgtggatt gataaagcgg aaacctgggc gctggcagat tattacggca
    11221 aactggattt agtccgtaac gaaacgttga cctgctataa cggcattaaa ggcgacggtt
    11281 gcggtcattg tgcggcatgt aatttacgcg ccaacggttt gaatcattat ctggccgata
    11341 aaccgacggt gatggcagcg atgaagcaga aaaccgggtt gaggtaatta ttccgggtgt
    11401 cgccggatgc ggcttgagca tccggcacca caaaacgttt acttaaccat ctgctccagc
    11461 ttttcgcgca attccccttc cagagctaat gctttctgcg ttttaagatc aatacaaaca
    11521 aacgtaataa gcgcatccgc taccacctgc ccttccggct ccagtgtaat gacctggttt
    11581 aagatgccgc ttttaccgtt taattgctgc aactgactgg taatagttaa caggtcgctt
    11641 aataccgctg gacgacgata gttaatattg atattgacca cgacgaaggc gatgttatgg
    11701 gccgtcatcc actgaaaact gtcgctattt tccaccccat cccagcgggc ttcttcgaga
    11761 aattcaaggt agcgggcgtt gttgacgtgc tggtaaacgt cgagatgata tccacgaact
    11821 ttgatttgtg tttgcatagc gcaataacct tacgttgttg ttataagtac agaggtcata
    11881 actggtatga cctcttcagt ctggcaaaaa attgccacta tgcaaattaa ttacagggtt
    11941 aataccgcca gattacgttc caccagcgaa ttgcccatcc ccggcacctg ctttagatcc
    12001 tccaccgttt taaacggacc gtactcttcg cgataactga caatcgcctg cgctttcttc
    12061 aggccaacgc cattcatcgc gcgggctagc tcttccgcgc tggcattatt aatgctgacc
    12121 cgggtgcctt cttcgtcact ggctttcgcc ggtactgctg ctttactttg tgctgcagga
    12181 gcttccgctt tggtttctac cgccgtcggt ttcgccacag aagctgccgc cagcgcgcta
    12241 tgagacattc cggcacaggc cagggacagt gtaatgagca gtgctttaat tccgtgtttc
    12301 atactgtttt ctccttgttt gttaacagtg gaatcacctt aacgggagtg aaaacagtaa
    12361 acaaatggca atgttcagaa atggaaaagg ccgcgaaagc gaccttttct tgttacagct
    12421 gcaacgcatt tttttgcgag gcggcttcag gattattgct gttccagcgc atcgccaatt
    12481 ttgattttcg cctctttacg caggttactc atcagagctt caaagacgat ttgtgcgttg
    12541 ttctgggtga taccctgcac catcgctttt ttctgatctt ccggcattga accttgtttc
    12601 acttcatcca gcgccagcag aaccacatta ccttgcatat cggtcgccat accgtagctc
    12661 ggtttgtctt tcgctggcag tggcagtgca aacgccgcct ggctaatcgg gtcacgaccg
    12721 gaacggctta aggttttcgg ctcgccaaat ttcagaccgg cagcctgcat agcttccgca
    12781 cctttgccgg ctttcaaatc aaccagcagt ttctcagcat ccactttcgc ctgttgttca
    12841 gctttgttgt gctgaaccag tgccttaact tgttcctgaa catctgccaa cggtttcacc
    12901 gcttccggtt tgtgctcgct gacgcgcagc acgaatgcgc ggtcgccgtc tacggtgatg
    12961 atgtcagagt tgatgcccga cgcgccgttt tcacctacca gaccgccgtt aaagatagcg
    13021 tcggcaaccg gcttgaagtt caactcttcc ggcaggttat ctttgctgaa ccaacccgtc
    13081 tgagtggctt taacgccggc agcttgctct gcaccggcca gagactcggt gtcgttgctt
    13141 gccgcatcgc tcactttctg ctgcagcgca tagtacgcat cgagggcttt ttcgtgtttc
    13201 actttcgccg caatgtcgtc acgtacttcg tctaacgatt tcactttcgc tggctgaatg
    13261 tcgtccagac gtacaatcag gaaaccgacc gaagatttga tgacaccaga cagttggcct
    13321 ttttctttca gaccagcatt tttcagttcg tcggggatag tggcatcttc taaccaaccc
    13381 atatcgccgc cgttacgggc agagataata tcggcagatt tttctttggc taatgcagca
    13441 aaatcaccgc ctttattcag ctcatcaagt accgctttcg cttcatcttc agttttggtc
    13501 tggatgatgc tgtagcgggt acgctgcggc tgggtgaatt gatcctgatg ctgatcgtag
    13561 tagctctgga tatccgcatc gctaaccggt tgctgcatcg ttgcggcaac cagcttgatg
    13621 taactcacgc ggaattgttc cggcgtcatg aaattgtttt tgttttgttc gtagtagctg
    13681 gcaatctcct gttcggtcac aggctgcttc gccgccagcg cgttaacatc gatagtcgcc
    13741 tcacgcacca cgcgttgttg cgcgaccagt gccgccagct cgtcggtttc acctttcagc
    13801 ataaaatcgg taccggcaac gccgttaatc agctgttggg tagtgagctg gttacgcagc
    13861 gcctgggcgt actgatcggc ggtcatcccc atctggttga ggataccgtt atagcggctg
    13921 ttatcaaatt tgccgtcaac ctggaaggct ggggtcgcga aaatcgcctg tttaacctgc
    13981 tcatcactga tacccagttt cagctcacga gcgtactgat ccaacagcgc ctcgtcgatc
    14041 agacgattca gcacctgttg acgcagggtt ttcatatagc cttcgttcgc tgccagctcg
    14101 gagtattgat cgcccagctg ttgctgcata cgattacgct cgctgttaaa ggcgttctcg
    14161 aactgcccac ggctgatttc ctggtcattc acttttgcgg cgtagttatt gcctccgcca
    14221 atcaggtaac cactcacgcc ggtcaatatg aacgacacga taatgatacc gaaaataatc
    14281 ttgagcacga gactgtttgc agccgtgcgt aagctgtcca tcatggtgta acaacactcc
    14341 gctgtagatg actgtgtacc tcacgcaacg tatcgatgcg cgcaaaggcc tattgtgaca
    14401 agaaacgggg gcaattgtca gcccacaaga cacagaaagt caccgaatac aaataaaaaa
    14461 ggcacatcag tagatgcgcc cttgaacttc gtcacatccc cactggggac aacgcttagt
    14521 ttaccgcgtc tttcagtgct ttacctgcac ggaagctcgg tactttagcg gcagcgatgg
    14581 tgatctcttt accggtctgc gggttgcggc cagtacgggc agcacgctct ttaacggcaa
    14641 aagtaccaaa acctaccagt gctacatcat ccccttcttt cagagattca gttacggaag
    14701 caataatagc atctaacgca cggccagccg cagctttaga gatatcagcc cctgcagcaa
    14761 tcttgtcgat caattgagat ttattcactc ttctcttcct ctttataatt tatatcgcac
    14821 ttgaatcctt caaggtacga acgcgcagca gttatatcag gccagccatc cccttacaac
    14881 accccttaat aagggcaagc ccgatccgct atctaactta gcgagacaaa aaaaggctgg
    14941 caagcccgaa ttagcctgcc agccctgttt ttattagtgc attttgcgcg aggtcactat
    15001 tttgcagtca caacctgcat gccagacggt tcattttgca gcgccagagt cagaacttcc
    15061 tcaatgcgct tcacaggatg aatgtccaga tcggcaatta cgttgtcagg aatctcttcc
    15121 agatcgcgtt tattttcgaa cggaattagc actgttttaa tcccgccgcg atgcgctgcc
    15181 aggagttttt ctttcaaacc accgatcggc agtacctgac cacgcagagt gatctcaccg
    15241 gtcattgcca catcggcacg aaccgggtta ccggtcaggc aagaaaccag cgcggtgcac
    15301 atagcaatac cggcactcgg accatctttc ggcgtcgcac cttccggtac gtggacgtgg
    15361 atgtcacgtt tttcgtaaaa atcagggttg atccccagtt tttccgcacg cgcacgaacc
    15421 accgttaacg ccgcctgaat agactcctgc atcacttcgc cgagcgaacc ggtataggtc
    15481 agtttgcctt tacccggaac acatgcggtt tcaatggtca gcaagtcacc gcccacttcc
    15541 gtccacgcca gaccgattac ctgaccgaca cggttttcgt tatcagcgcg accatagtcg
    15601 aaacgctgaa caccgaggta gtcatgcagg ttatcgccgt taatttcgat atgttttaat
    15661 gacttatcga gcagtaactg cttaaccgct ttgcggcaca gtttggagat ttcacgctcc
    15721 agaccacgca cgcccgcctc acgggtgtag taacgaataa tgccgataat ggcgctatcg
    15781 tcgacggtca gctcaccttt tttcagtgca ttacgttcaa tctgcttcgg cagcaggtga
    15841 cgtttggcga tgttcagttt ttcatcttcg gtataaccgg agaggcgaat cacttccata
    15901 cgatccagca gcggtgccgg aatgttcatg gagttcgacg tcgcgacaaa catcacgtcg
    15961 ctgagatcgt aatccacttc caggtagtgg tcgctgaacg ctacgttctg ctctggatcc
    16021 agcacttcaa gcagtgcaga ggccggatcg cctcgcatgt cagaagacat tttgtcgatc
    16081 tcatcgagca ggaacagcgg atttttcacg cccactttcg ccattttctg gatcagttta
    16141 cccggcatag aaccgatgta agtacggcgg tgaccacgga tttccgcttc atcacgcacg
    16201 ccgcccagcg ccatacggac atatttacgc ccggtggctt tggcaatgga ctgaccaaga
    16261 gaggttttac ctacccccgg cggccctacc aggcagagga tcggtccctt gattttgttg
    16321 acacggcttt gaaccgcaag gtactcaagg atgcgatctt tcacgcgctc cagaccataa
    16381 tggtcggtat caaggatttc ctgcgcctga cgcaggtctt ttttgacctt gctgcgcgca
    16441 ttccacggta cctgtaccat ccagtcgata taaccacgca ctacggtcgc ttctgccgac
    16501 atcggagaca tcattttcag cttctgcaac tctgcttccg ctttctcttt tgcctctttc
    16561 ggcatcttcg ccgcgtcgat tttgcgcttc agggcttcgt tttcgtccgg cacgtcgtcc
    16621 atttcaccga gttctttctg aatagctttc atttgctcgt tcagatagta ctcacgctgg
    16681 gatttctcca tctgcttttt aacgcggttg cgaatgcgtt tctcaacctg cagcagatcg
    16741 atttccgatt ccatcattgc catcagatat tccagacgtt cgttaacgtc ggacatctcc
    16801 agaacggact gtttgtcagc cagtttcagc ggcatatgtg cagcaatggt atccgccaga
    16861 cgcgccggat cgtcgatgct attcagcgac gtcagcactt ctggtgggat ttttttgttc
    16921 agcttgatgt agccttcgaa ctggctgatt gcagtacgca ccagcacttc ctgttcccgc
    16981 tcatcaatgg tcggcgactc cagatactcc gccttcgcag aaaagtgttc gccattgtca
    17041 gagagcgcag aaatacgcgc gcgctgtaac ccctcgacca gcactttgac ggtgccgtca
    17101 ggcagtttca gcatctgcaa tatagaggcc acggtcccga cggtgaaaag atcgtttaca
    17161 cccggctcat ccgttgaagc ttctttctgc gcgaccagca taattttttt atcatggtcc
    17221 atcgccgctt ccagacaacg gatagatttt tcccgcccga caaataaggg gatgaccatg
    17281 tgcggataaa ccaccacatc gcgcagcggc aatacgggga tttcaatgcg ttcagaacgc
    17341 tcaggattca tagagctctc tcttagttta atttccgcca ggtaatcaga tgacacgact
    17401 gtgcttcacg ccatctatta acatgtacgt cagtatatgg ggatgtttcc cccacattca
    17461 acgccgagaa tagaggaaaa attaaagggg agataaaatc cccccttttt ggttaactaa
    17521 ttgtatggga atggttaatt attcaccaga tgcctgttgc gcttccggct taccataaat
    17581 cagcaacggt ttgctttgac catcaattac cgactcgtcg ataaccactt tttcgacgtc
    17641 ttccatggac ggcagatcgt acatggtatc gagcagtgcg gcttctacga tggaacgcag
    17701 gccacgggca ccggttttac gcgccatcgc tttcttagcg atagcatcca gcgcctcgtc
    17761 acggaattcc agatccacgc cttccagatt aaacagcgcc tgatactgct tggtcagggc
    17821 gtttttcggc tctttgagga tctgaatcag agcttcttcg ctcagttcat tcaacgttgc
    17881 tacaaccggc agacgaccaa tgaactcagg gataagacca aacttgatca gatcttccgg
    17941 ttcaacctgc gccagcagct cgccttcgct tgctttgtcg gacttcgctt ttaccgtcgc
    18001 gccaaaacca atgccggagc cggtttctac acggtgggaa atcactttat ccagaccggc
    18061 aaacgcaccg ccacaaatag acaggatctt agaggtatca acctgcaaga attcctgctg
    18121 cggatgttta cgcccacctt gcggtggaac agcagctacc gtaccttcga tcagtttcaa
    18181 cagtgcctgc tgtactcctt caccggaaac gtctcgggta atggacgggt tgtctgactt
    18241 acgagaaatc ttgtcgattt catcgatgta gacaatcccg cgctgcgctt tctgtacgtc
    18301 gtaatcgcac ttttgcaaca gcttctgaat gatgttttca acgtcttcgc ccacataacc
    18361 ggcttcggtc agcgtggttg cgtcggccat ggtgaacggg acgtccagca ggcgcgccag
    18421 cgtttcagcc agcagcgttt taccggaacc ggtcggaccg atcagcagaa tgttactttt
    18481 gcccaactcg acaccattgc tggtatcgcc gttgcgcaga cgtttgtaat ggttgtatac
    18541 cgcgaccgcc agcacttttt tcgcctgttc ctggccgata acgtaatcgt ccaggtggtt
    18601 gcgaatttca tgcggcgtcg gtagcgcact gcgttcacga tgcggtgcaa cttctttaat
    18661 ctcttcgcga atgatgtcgt tacataaatc aacacattcg tcgcagatat acacggatgg
    18721 accggcaatc agcttgcgca cttcatgctg gcttttgccg caaaaagagc aatacagcaa
    18781 tttgcctgag ccatctttgc gtttatctgt catgggtcaa aacctcttct ttgttctttg
    18841 tgccgcacac gacgacgcaa atgccattct caggcgcaag ccgcttacag cgttgtgccg
    18901 ccctagataa gtatagcggc acagttgcgc ctctggcatc aattacgatg ggtcagaatc
    18961 gaatcgacca gaccgtattc caccgcttca ggggcggaaa ggaagcgatc gcgctcggta
    19021 tcacgttcaa tctgttctaa tgattgaccc gtatgaagcg ccataagttc attcatgcgc
    19081 cctttaactt tcagaatttc acgggcatga atttcgatat cggtcgcctg gccctggtag
    19141 ccgcccaacg gttggtgaat catcacgcgc gaattcggca ggcaaaaacg tttacctttt
    19201 gcccctgcgg tcaacaagaa agcgcccatc gaggccgcct ggcccataca gatggtgctg
    19261 acatcaggct tgataaactg catggtgtca tagatagaca tcccggcagt gatcaccccg
    19321 cctggggagt taatgtacag atagatatct ttttctgggt tttccgcttc caggaacagc
    19381 atctgcgcca caatcaggtt agccatgtgg tcttcaacct ggccagtcag aaaaatgacg
    19441 cgttccttaa gtagacgaga atagatatca aaagagcgct caccgcgtga ggtctgttca
    19501 atgaccatcg gcaccagcgc catatggggt gcaaagttat ctcgttcgcc gctgtatgac
    19561 atttccgtct cctggataaa attgaaaaaa acctgctgta ccgattgtaa ccttatggac
    19621 ggattatcag ctagtccctg tgttatgggt acggcatcac catatttcaa gcataacaat
    19681 cttttgttgt tacgctaaca ccgaaagagg cttttcgcac ggctccatgc aaaattcatg
    19741 acaaaaaaaa gcccaccacc tggcggtgac gggcctttgt gcgaatttag cgcgttatgc
    19801 tgcgtaaatt acgcctgctg gttcatcagc tcgttgaaag tggtttcttt ttcagtcact
    19861 ttcgctttcg ccagtacagc ttcaacagcc tgttcttcca gagcaacatt gcgcatgttg
    19921 tccatcagtt ctttgttttt gctgtagaac tcgataactt ctttcggatc ttcgtacgca
    19981 gaagccatct cttcgatcag gcctttcacg cgctcttcgt cagctttcag ctcgttggtg
    20041 cggataactt cgcccagcag caggccaaca actacgcggc gtttagcctg ttcttcgaac
    20101 agttcgcgcg gcagttccag agcttgtttt tcgttgccac cgaaacgctg tgcagcctgg
    20161 cgacgcagaa cgtcgatttc gctgtcgatc agcgcagccg gtacgtcgat gtcgttagct
    20221 tttaccagac cttcgatcgc ctgagactta acgcggttac ggatggcgct cttcagctcg
    20281 cgctccatgt ttttacgcac ttcagcgcgc agaccttcta cggaaccatc ttcaacgccg
    20341 aaacgtttga tgaattctgc agtcagttcc ggcagttcac gctcttcaac tttcttcagg
    20401 ttgatagcga atttcgctgc tttacctttc aggttttctg cgtggtattc ttccgggaag
    20461 gtcacgtcga tggtgaactc ttcgccagct ttgtggcctt tgataccgtc ttcaaagccc
    20521 gggatcatac gaccctggcc catcgccagt acgaaatcag acgctttacc gccttcgaac
    20581 tcttcgccgt ctacagaacc ggtgaagtcg atggttacgc ggtcttctgc ttcaacagcg
    20641 ccgtcttttt ctttccaggt cgcctgctgt ttacgcagag tatccagcat gccgtcaacg
    20701 tcagcgtcgg tcacttcaac gatcggtttt tcaacttcga tcgcttccag accctgcagt
    20761 tcaacttccg gataaacttc aaactctaca gagtaagtga agtcttcacc cagcttgtat
    20821 tcgcccggaa cataagtcgg tgcgccagcc ggattgattt tttctttaat gatggcgtca
    20881 atgaagttac ggctcatcag gtcacccaga acgtcctggc gtacagacgc gccataacgc
    20941 tgagcaacga tattcattgg cactttgcct ttgcggaagc cgtcaatacg tacttttttc
    21001 gcaacgttga ccagctcgct tttaacagcg gtctcgatgc tgtcagcagc gatagtaatc
    21061 gttacacggc ggccaaggcc ttgagtggtt tcaactgaaa cttgcatctt gttacctcaa
    21121 aaaatcacag tgctcggtca actcttaccc cgcaagcaca ggttgttggc ttcgcggaac
    21181 cgggatgttc ttataatcaa tcacattccc tgttaccaga atcatcccga aagcattcaa
    21241 ataggacgcg gcattatagc ggcatcactt ttatgagtcg agaacggtta ttgcgcgctg
    21301 tcgcgtattt tttcacaatt cccgggcaat tttcgtctga atgctgggca aaaagcgcaa
    21361 aatccagaca aaacaaaacg gcccgcaggc cgtttttcat aaaatcttta gcaacatact
    21421 ggaaaagcgc cgacagttgc aaatgcgttt ttacgcgatg cttcctgctc cacgacaggg
    21481 aggagaggca aagacggtgt cctgcaaccc ttcccactcc ttaatagtgt aagtatgcag
    21541 agccagcgca tgaacggtag tagagagttc ctccgctaaa gtactgtaaa tcattcgatg
    21601 acgattcaga aaacgttcac ccgtaaaacg atcgctgacc agcacaactt taaaatggct
    21661 ttcagagccg gctgggacat tgtgacgata gctttcatcc actacttcga ggaatacggg
    21721 ttggaacgcc gcccttaatt tttcttctat ccgctcacgt atcatcatga aatatctcct
    21781 ccgacaacgc tgagatgtca tccatccctt taaatactag ccgcttttac cgtttccatt
    21841 gcagcttaac aacaaatatt taggtttcgt cctggttttt ccgtcaagcg tatgaagttt
    21901 actgatttaa agattgaaac acaggcctcg catcagcgtt tcttttacca ggggtgatta
    21961 tttcgtcaac atgatgaatt tacatgcctt acccacttcc cctcgccgtt ggcaatgtta
    22021 tgatggcggg aatttttacc aaacacgctt aagagtctct gagagcccga acatgttcaa
    22081 aaaaatcctc ttcccgttag ttgctctgtt tatgcttgca ggatgcgcaa aaccgccaac
    22141 aactattgaa gtttccccga cgattacact gccacagcag gatccaagcc tgatgggcgt
    22201 caccgtaagc attaatggtg ccgatcagcg taccgatcag gcgctggcaa aagtcacccg
    22261 cgataatcaa atcgttaccc tgaccgcctc ccgcgctctg cgtttcctgc tgcaagaagt
    22321 gctggaaaaa cagatgaccg cgcgtggtta catggttggt ccgaatggcc cggttaatct
    22381 gcaaatcatc gttagccaac tgtatgctga cgtgtcccag ggcaacgtgc gctacaacat
    22441 cgctaccaaa gcggatatcg ccatcatcgc taccgcgcag aatggcaaca aaatgaccaa
    22501 aaactatcgt gccagctaca acgttgaagg tgcgttccag gcctccaaca aaaatatcgc
    22561 cgatgcggtt aacagtgtgc tgaccgatac catcgctgat atgtctcagg acaccagcat
    22621 tcacgaattc atcaagcaga acgcgcgtta atttctgccc tctggcccgg tgcaagccgg
    22681 gcctgtagac gcccatgtcc agtcaatatt tacgtatttt tcaacagccg cgttcagcca
    22741 tattgctgat cctgggtttt gcttccgggc taccgctcgc cctgacatcc ggcaccttac
    22801 aggcctggat gacggtcgaa aatatcgatc tcaaaaccat tggtttcttc tctctggtag
    22861 gccaggctta cgtttttaaa ttcctctggt caccgctgat ggaccgctac acgcccccat
    22921 tttttgggcg gcggcgcggt tggctgctcg ccacgcaaat cctgttatta gtcgccattg
    22981 cggcgatggg ttttctcgaa ccaggcaccc aactccgctg gatggcggcg ctggcggtgg
    23041 tgatcgcttt ttgctctgcc tcccaggata ttgtcttcga tgcgtggaaa accgatgtgc
    23101 tcccggcaga agaacgtggt gcgggcgcgg caatcagcgt gctgggttac cgtttaggga
    23161 tgctggtttc cggcggccta gccctgtggc tggcagataa atggttgggc tggcagggca
    23221 tgtactggtt gatggcggcg ctgttgatcc cctgtattat cgcgacgttg cttgcaccag
    23281 aaccaaccga caccattcct gtgcccaaaa cgctggaaca agcggttgtt gcacctctgc
    23341 gagatttctt tggtcgcaat aatgcctggc ttattttgct tcttatcgtg ctgtataagc
    23401 tgggcgacgc attcgccatg agcctgacaa ccacgttttt gattcgcggc gtcgggtttg
    23461 atgcgggtga agtaggcgtg gtaaacaaaa cgcttggctt actggcgacc attgttggcg
    23521 cattgtacgg tgggattttg atgcagcgcc tgtcgctgtt ccgggcgctg ctgattttcg
    23581 gcattttaca aggtgcgtct aacgctggtt actggctgct gtcgattact gataagcatc
    23641 tctacagcat gggcgcagcc gtctttttcg aaaacctctg tggcgggatg ggcacatcag
    23701 cctttgtcgc gctgttaatg acgctgtgta ataagtcatt ttccgctact caatttgccc
    23761 tgctctcagc gctttccgct gtagggcgag tttatgtcgg ccccgtggcg ggttggtttg
    23821 ttgaagcaca cggctggtcg acattctatc tattctccgt cgccgctgcc gtaccggggc
    23881 ttattttgct gctggtttgc cgccagacgc ttgaatatac acgagtaaat gacaacttta
    23941 tctcccgtac cgaatatccg gcaggttatg cctttgccat gtggacactg gcggcgggcg
    24001 tcagcctgtt ggccgtgtgg ttacttctgt tgacgatgga cgcgctggat ttgacgcact
    24061 tctctttcct gcctgctctg ctggaagtcg gggttttagt cgccctttct ggcgtcgtgc
    24121 ttggtggttt gctggattat ctggcgctac gaaaaacgca tctgacgtaa tctgtaaata
    24181 ttatttagct gtagtaatca ctcgccgtaa ttattacggc taaataaaca tcagcatcgc
    24241 ttattaatat ttgttctttt gtgcggctta gcgtttggaa attattgacg ccatttataa
    24301 attctatttt tcttacacga ttcagctaat gagtctttat tttctcacca cccagttgtc
    24361 actttaatga taattatttg ttaaataatt gttttatttc gcattggtta taccaattgc
    24421 ccaccaagac tccttctgca ctcccctttt gtta
//
LOCUS       DAJTSF010000061        24089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000061 DAJTSF010000000
VERSION     DAJTSF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.061"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..178
                     /gene="yjbD"
                     /locus_tag="OO335_002519"
     CDS             <1..178
                     /gene="yjbD"
                     /locus_tag="OO335_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR6002866.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="OO335_002520"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="OO335_002520"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6002867.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="OO335_002521"
     CDS             1531..2478
                     /locus_tag="OO335_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR6002868.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="OO335_002522"
     CDS             2548..3354
                     /locus_tag="OO335_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6002869.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSENGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="OO335_002523"
     CDS             3364..3771
                     /locus_tag="OO335_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6002870.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3771..4265
                     /locus_tag="OO335_002524"
     CDS             3771..4265
                     /locus_tag="OO335_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR6002871.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4331..5128
                     /locus_tag="OO335_002525"
     CDS             4331..5128
                     /locus_tag="OO335_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6002872.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5139..5927
                     /locus_tag="OO335_002526"
     CDS             5139..5927
                     /locus_tag="OO335_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6002873.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5979..7208
                     /gene="sorE"
                     /locus_tag="OO335_002527"
     CDS             5979..7208
                     /gene="sorE"
                     /locus_tag="OO335_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR6002874.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7590..8279
                     /gene="pepE"
                     /locus_tag="OO335_002528"
     CDS             7590..8279
                     /gene="pepE"
                     /locus_tag="OO335_002528"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6002875.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8370..10001)
                     /locus_tag="OO335_002529"
     CDS             complement(8370..10001)
                     /locus_tag="OO335_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6002876.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10221..13904)
                     /gene="metH"
                     /locus_tag="OO335_002530"
     CDS             complement(10221..13904)
                     /gene="metH"
                     /locus_tag="OO335_002530"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR6002877.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEQGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14104..14928
                     /gene="iclR"
                     /locus_tag="OO335_002531"
     CDS             14104..14928
                     /gene="iclR"
                     /locus_tag="OO335_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6002878.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15245..17429
                     /gene="espL4"
                     /locus_tag="OO335_002532"
                     /pseudo
     CDS             15245..17429
                     /gene="espL4"
                     /locus_tag="OO335_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="OO335_002533"
                     /pseudo
     CDS             complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="OO335_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="OO335_002534"
     CDS             complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="OO335_002534"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR6002879.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="OO335_002535"
                     /pseudo
     CDS             complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="OO335_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22523..23452)
                     /gene="metA"
                     /locus_tag="OO335_002536"
     CDS             complement(22523..23452)
                     /gene="metA"
                     /locus_tag="OO335_002536"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6002880.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23609..24052
                     /locus_tag="OO335_002537"
     CDS             23609..24052
                     /locus_tag="OO335_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6002881.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5945 a         6146 c         6108 g         5890 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaaatggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtggaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     4201 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     4261 attaaataat agcgcctgtg tacatatgct ttaacaggca gggatgacga cacttaaaag
     4321 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     4381 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     4441 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     4501 attgatagct ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     4561 cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc
     4621 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac
     4681 cgtagtgttc cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga
     4741 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat
     4801 tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     4861 tgtgacccga ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     4921 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     4981 tggctacctc gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     5041 ctacatccag ctaaatccac agtggtgtaa agctgaacct caactccagg ccaccgcctc
     5101 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     5161 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     5221 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     5281 cattaaaaga ggatcaggtt gcggcgctca ggctacacct ggtgttcttc aataccacgc
     5341 cagccgtatg tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca
     5401 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     5461 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc
     5521 tgctgttctt ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc
     5581 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     5641 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga
     5701 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     5761 ccatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     5821 cgctactaat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     5881 tgtttggctt agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg
     5941 cacgactgcc gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
     6001 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
     6061 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
     6121 tgggtgaaaa ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc
     6181 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
     6241 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
     6301 actccttccc gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
     6361 aacaatattg cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac
     6421 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
     6481 accacacgat ggggattcgc ccgcaagggc acacgctgat cctcggcggc accggaccaa
     6541 tgggactgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca
     6601 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
     6661 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga
     6721 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
     6781 tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata
     6841 aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
     6901 gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa
     6961 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
     7021 cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc
     7081 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc
     7141 aggggatctg gtccggtgag gtggagcaat acctgctcgc ccatgcagag gcaatttccc
     7201 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg
     7261 accggatgtg cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc
     7321 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
     7381 atgaagcctt attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag
     7441 gccaggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
     7501 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
     7561 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     7621 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
     7681 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
     7741 agaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     7801 tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt
     7861 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta
     7921 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     7981 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
     8041 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
     8101 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc
     8161 cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     8221 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag
     8281 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
     8341 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
     8401 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct
     8461 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gcacgttttg ctgatgcagg
     8521 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga
     8581 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
     8641 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
     8701 cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag
     8761 gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc
     8821 gccaactctt cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg
     8881 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
     8941 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
     9001 ttcgccagag caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc
     9061 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
     9121 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
     9181 agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc
     9241 aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct
     9301 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca
     9361 atcaccagac agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc
     9421 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
     9481 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
     9541 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
     9601 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
     9661 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
     9721 gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc
     9781 tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
     9841 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
     9901 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
     9961 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcaga ttctccttat
    10021 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    10081 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    10141 taagtgtttc atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg
    10201 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    10261 tcaacatcgg agacgctcat acctttacgg cgggcatggt cttcaacctg atcgcgctga
    10321 atttgcgcca ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat
    10381 gcaccaggcc acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    10441 agcagctccc agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc
    10501 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc
    10561 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    10621 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag
    10681 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    10741 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    10801 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    10861 cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc
    10921 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    10981 cgctgcgcct caacgcccac cacttcatct tccagaatgc gaggatactt cccggccagc
    11041 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct
    11101 tccacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat
    11161 gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc
    11221 ccgtgctgaa tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc
    11281 tgagtatcgg aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac
    11341 accgtagggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc
    11401 gcgccgccaa tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta
    11461 accatctcgt ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact
    11521 tctttggcga tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    11581 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct
    11641 ttcacggtgg cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata
    11701 aacggttcga gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    11761 aggaacattt tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    11821 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg
    11881 ataaactcgg ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag
    11941 cgccactccg cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg
    12001 gcaagctcca gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc
    12061 gcgtcgcgca gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    12121 cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc
    12181 accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    12241 tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg
    12301 tgctcttcaa taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc
    12361 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    12421 gcgcgagtat cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag
    12481 cgacgcaaca atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag
    12541 ttaacaatgc ctttgccctg gatacacttc agaccttgtt caatgacatc ccattttgag
    12601 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    12661 cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc
    12721 gcgccgtttt ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg
    12781 atcaggcgct tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac
    12841 aggctatcgt cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt
    12901 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc
    12961 ggcgtggtcc cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt
    13021 atctgttttg ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg
    13081 gcgttcgggt gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg
    13141 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    13201 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    13261 atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc
    13321 gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    13381 gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa
    13441 gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    13501 gggccaagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    13561 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    13621 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg
    13681 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    13741 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    13801 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata
    13861 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    13921 cttaacccag atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa
    13981 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    14041 ctattgcatt agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct
    14101 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    14161 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    14221 tccaatggca gcgtggcact cacagagctg gcgcaacaag ccgggttacc caattccacg
    14281 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg
    14341 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    14401 aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    14461 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag
    14521 tgtacccacc tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttcc
    14581 ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    14641 cgcaaagggt tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa
    14701 gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg
    14761 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct
    14821 atttccggac cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg
    14881 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    14941 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    15001 tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca
    15061 ttatttttaa acatcaaacc acttaattat taacaaaatc gcaagatctc attccaccga
    15121 aacttttacg gctgcattat gagattgagc gatttatgat atttttttag ttgtaatcat
    15181 attaaatttt ttcactgttc tgatagttaa aattcaagac atcaataaac aatgagatat
    15241 ttaaatgatt actcgtattc ctcgtagttc tttctctgca aatattaata atacagccca
    15301 gacaaatgaa caccaaaccc tgagtgaatt gttttataaa gaactcgagg ataaattttc
    15361 tggcaaggag ctggcgacgc ctctattaaa aagcttctca gagaactgta gacaaaatgt
    15421 tcgtcatatc tttagcaaca aggattttgt cattaaattt tccacgtccg tcttacaagc
    15481 tgataagaaa gaaattacga tgattaataa aaacgaaaac acgacactca ctcaaaccat
    15541 tgctacaata tttgaagaat acctaatgga aattttacct caacgctcag acactcttga
    15601 taaacaagaa ttaaaccaaa aatcagatag aaaagaaaaa gaattcccaa gaattaaact
    15661 taatggtcaa tgttatttcc cggggcgccc caaaaccgta tagtataccg acacattgct
    15721 gcacaatata ttaatgatat ttatcagaat gttgattaca aaccccatca agatgattac
    15781 tcttcagctg aaaaatttct cactcacttc aacaaaaaat gcaaaaacca gactttggcg
    15841 ttgatttcca gccgtcctga ggggcgttgc gttgctgcct gcggtgattt cgggctagtt
    15901 atgaaagcat attttgacaa gatggaatca aatggcatca gtgttatggc agccatatta
    15961 ctggtggata accatgcttt gacggtccgg ctaagaataa agaacacaac tgaaggatgt
    16021 acccattacg tggtttcggt ttatgatcct aatgtaacta acgataaaat aagaattatg
    16081 agcgaaagca aagaggatat taaacactat tctctgatgg attttatgaa tgtagattat
    16141 agcctcctga aatggtcaaa tgatcatgtt attaaccaat ctgttgcaat aattccagca
    16201 cttccgaaag aacagctatt gatgttaaaa ggaactgtgg atgaaataac ccctccatta
    16261 tcaccagcaa cgatgaattt gctaatggca attggtcaga atcaccaact tacgcaactg
    16321 atgattcagc tccagaaaat gccagaacta catagaacag aaatgttgac tgcctataat
    16381 agtattaacc tccccggttt atatttggct ataaattatg gcaatgcgga tatcgttgag
    16441 actattttca attcattgtc agaaacagga tatgaagggt tactctcgaa aaaaaatctc
    16501 atgcatattc tggaggcaaa agataaaaat ggtttttctg gattattttt agcgatatca
    16561 cgtaaggata aaaatgttgt aacctcgatt ctgaacgtct tacctaaact ggccgcaaca
    16621 catcatttag ataatgaaca ggtgtataaa ttcctgagtg ccaaaaatag aacgtccagc
    16681 catgttttat accatgttat ggcgaatggt gatgccgaca tgctgaaaat ttttttggtc
    16741 gcgttacctt tgttaattcg cacatgtcat ttgactaaag aacaggtact cgatctcctg
    16801 aaggcaaagg atttttatgg ttgcccaaga ctatacctgg cgatgcaaaa tggacatagc
    16861 gatatcgtga aagttattct cgaagcattg ccctgtctgg cccaggaaat taacatttca
    16921 gcttccgata ttgtagatct tctgaccgct aaaagtcttg cgcgcgacac gggtttgttt
    16981 atggccatgc agcgcggaca tatgaacgtt attaatacta tttttaacgc attacccact
    17041 ctttttaata cgtttaaatt cgataaaaaa atatgaagcc cctcctcctg gcaaataatt
    17101 ctaatgaata tcccggtttg ttttcagcga tacagcataa acaacaaaac gttgtagaga
    17161 cggtttatct tgctttatct gaccatgcac gcctgtttgg atttaccgct gaagatatta
    17221 tggatttttg gcaacacaaa gcgccacaaa aatactctgc ctttgagttg gcttttgaat
    17281 ttggtcaccg ggttattgct gaattaatcc ttaatacatt aaataagatg gctgaaagct
    17341 ttggctttac ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca
    17401 aaaaagcatc tccccatacc gtacgctaaa tctttgcctg cgccgatacg cataaacatc
    17461 ttccacatgc ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag
    17521 gtcggcgtgc atctcttcaa acagcggacc aatacgcggg tcggcgcata gccagtggcg
    17581 aaactcttcc gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc
    17641 gtcttccgga tagcgcggcg gcgggatgtc gcggaaattc acttccgtca tgtagcaaat
    17701 ttcatcgtaa tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag
    17761 catgtcgcca gggaaaatgt tagcagcggc aagctggcga atagcgttac cgtattcttc
    17821 aatggcgtcg cgcaactgct gaccttccac ttgttccagc cagatattga gcggcaccat
    17881 ccgccgctcg attatagaga tggcgaatta caatctgctc accaagatcg gtgatttttt
    17941 ccgctgcttc ctggagcagt aattccatta ataccgggga aatatgccgc ttctccagca
    18001 caaagttttc aaactcctgg gtgtccgcca ttcggcccac acggtcgtgc tctttcacca
    18061 gttgatagca ggcacgaacg tgagcggcag acatctcttt ctgcggcgcg aacttgtctt
    18121 tgatgacttt gaatacccga tcaaagcccg gcagcgtaaa caccaacatc accattccac
    18181 gaatacccgg cgcttcaatg aactgctcat tacagccctg tagataaacg agatattcgc
    18241 ggtagctttc ggttttggcg tgcttctggc agccgatagc catatacaat tcagcagtgg
    18301 ttttacctgg cagaatttcc cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa
    18361 ccataaaata agaacgcgca aagccaaaaa caatgctcgc ttcggcggtc gtcgtcaggc
    18421 aggtatcaat aaataactcg ccgtcgtccg tctggtggat cggcagtaag aagggcaacg
    18481 tgccggaagg cgtgatcagt ttgcccacca gccaggcggc tttattgcgg taaaacagtt
    18541 cgttcgccac ctgtaaatga ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat
    18601 ggcgaatgat gtaatgaatg tcacggcttt tattctgcca gcgcaggcgc agcggcaggt
    18661 cgctgataac gcgcatcagt agagattccc agccgtgatc ggggtgaaag tctttcgcca
    18721 gcgggcgggg aatggtacga aagcggcgct ctggctgaga gctaaagata aaaagccgct
    18781 cgggagtaag cgagcggtgg tcaaataacc gacagtacac ggagttaaaa aagctctccg
    18841 caatctcgaa gcgcgggtaa tccggcaaca gccgcgtgta atgctcttta acgcgcagta
    18901 aaaatgccgc gtccgtactt tggccgttag taatgcagcg cagttgctcc acgaccagac
    18961 caacgtgatg atcgtaaaga tggatacggt ttttcatcgc ctgctggaca gcatgccagt
    19021 cggcctgttc gaaacgctgc tgcgccccgg aagtcacttc gaggaatcga ccatactgag
    19081 cgtcgaagcc ttgcaaaatg gtttgagcaa tcagtaattc caggccacgc ggcatttagc
    19141 gccctcatca ggagcagaga attgccggat gcggcgtaaa cgccttatcc agcctacgtt
    19201 cggcaacggt tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat
    19261 tgccggatgc ggcgtaaatg ccttatccgg cctacagtca gcaacgtttg taggttgctt
    19321 agaactgcga ttcttcagtg gagccagtca gcgcggtgac tgaagacgtg ccgccctgaa
    19381 taatagtcgt cactttatcg aagtaacctg tacccacttc ctgctggtga gatacgaagg
    19441 tatagccatc tttcgcggcg gcaaattccg gctgctgcac tttctcaaca tagtgcttca
    19501 taccttcgcc ctgcgcataa gcgtttgcca ggtcgaacat gttaaaccac atgctgtgga
    19561 tacctgctag ggtgatgaac tggaacttgt agcccatatc cgacagctgc tgctggaagc
    19621 tggcaatagt tttgtcgtcg aggtttttct gccagttgaa cgacggcgag cagttataag
    19681 ccagcagttt gcccggatat ttcgcgtgga tagcttgtgc aaagcgacgc gccagttcca
    19741 gatccggcgt ggaggtttca caccagacca ggtcagcata tggcgcatac gccaggccac
    19801 ggctgatcgc ttgctcaatg cccgcatgag tacggaagaa gccttcactg gtacgctcgc
    19861 cggtaataaa ttcgctgtca tacgggtcgc aatctgaggt gatcagatcc gccgcatcag
    19921 catcggtacg tgcaaccagc agggttggaa cgcccgtcac gtcagctgcc agacgcgccg
    19981 cgaccagttt ctgaatagct tcctgagttg gcactaaaac tttgccgccc atgtgaccgc
    20041 atttcttcac tgacgccagc tgatcttcga agtgaactgc cgcagcaccg gcttcaatca
    20101 tcgctttcat cagttcaaag gcattcagga caccgccaaa accggcttcc gcatcggcaa
    20161 cgatcggcag gaagtaatcg acatagcgcg gatcgcccgg ctcaatgccc acggaccatt
    20221 ggatctgatc ggcacgacgg aaggtgttgt tgatccgctc caccacagct ggcaccgagt
    20281 ttgccggata gagcgactga tccggataca tgctggccgc caggttagcg tccgccgcta
    20341 cctgccatcc cgacagatag actgcttcaa tacccgcttt cgcctgttgc agcgcctgac
    20401 cgccagtcag tgcaccgagg ttgttgatgt agcctttttt cgactcaccg tgcagcagac
    20461 gccacatttt cgctgcgccc agttgcgcca gcgtgcattc aggattgact gaaccgcgta
    20521 atttcaccac atcttccgca ctgtatgggc gagtaatgcc ttcccaacgc ggttgagtcc
    20581 actctttctg taattcttca atttgttgtg tacgggtttt catgtgcaga tgctccatag
    20641 ttatgtggtg gtttacgcta acaggcggta gcctggcagg gtcaggaaat caattaactc
    20701 atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg tcaaaacggc cctgggagaa
    20761 acgttcttcg cccagttcgc tggcaatgac tttcatctct tcgcccagca tctggcggaa
    20821 caaggctttg gtcaccggtt tgccattgct caacgttttt tgatgatgga tccactgcca
    20881 gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc atcaggccat aaatcggcac
    20941 gcagccgttg ccggagatcc acgcttcgat gtactgcaca gccacgcgaa tgttagcgcg
    21001 cataccttct tcggtgcgtt caccatcaca aggtgccagc agctgatcgg cagtaatcgg
    21061 cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg gagccgagaa tgtcgttgaa
    21121 taccgccatt gccgtgtccg caaggcctgg gtgagcgatc catgtgccat cgtgaccgtt
    21181 attggcttcc agcgctttat ccgcttttac tttgttgagc acctggttat tacgctcttc
    21241 atctttgctc ggaataaacg cggccatgcc gcccatcgca aaagcaccgc gtttatggca
    21301 ggttttaatc agcaggcgtg agtaagcatt caggaatggt ttatccatcg tcacggcctg
    21361 tctgtctggc aggacacgat cgggatagtt tttcaacgtt ttgatatagc tgaagatgta
    21421 atcccagcga ccgcagttca gaccaacaat atggtcgcgc agcgcgtgaa ggatttcgtc
    21481 catctggaac acggcgggca gcgtttcaat cagcaacgtc gccttgatgg tgccgcgcgg
    21541 cagattaaag cgatcttctg catagctgaa gacttcgctc caccaggccg cttcctgcta
    21601 ggactgggtt ttcggcagat agaaataggg accgctgccc tttgccaaca gtgcctgata
    21661 gttgtggaag aaatagagcg caaaatcaaa caggctaccg gggattgcct caccacgcca
    21721 ggtgacatgt ttttccggca agtgcagacc gcgtacccga caaatcaaaa ccgctggatt
    21781 gggcttgagc tgataaattt tgcctgcttc attggtatag ctgatggtac cgttaaccgc
    21841 atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag tctggtgcca gtgaatcttc
    21901 gaaatcggcc ataaagactt tcacattggc gttcagtgcg ttgatcacca tcttgcgctc
    21961 taccgggcca gttatctcta cgcggcggtc ttctaagtcc gcaggaatcc cgcgaatttt
    22021 ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa tcaggcaacg ttccgttatc
    22081 aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta ttgcgttgtg gcgtaaaatg
    22141 cgtcaccagc tcagtcagaa attctaccgc ttcggcagta agaatttgct tctcctgctc
    22201 gccatacggc cttgtgaaag ccagttcatc ggttgttgtt gcctgttcag tcatcgtgca
    22261 gctcctcgtc atggatccga aaacttcccc tctgtgaacg aaggaacgtt gtgcgctttt
    22321 cgttcagcac aactaagact actcatttaa aatgcaaaat caaaaacaat ttccatttta
    22381 attaaaaata cttgataact tattgataac aatatgaata aaatttattc acttgctgag
    22441 gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga ggtaaggtgc tgaatcgctt
    22501 aacgatcgac tatcacagaa gattaatcca gcgttggatt catgtgccgt agatcgtatg
    22561 gcgtgatctg atagacgtaa tagttgagcc agttggtaaa cagcaaatta ccgtgactac
    22621 gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg gaaatagtta tacggtacat
    22681 ccgggtctag tccggcttcc acatcgcgta aatattcctg cgccagcgtt tgcgcatcat
    22741 attcgggatg gcccgtcaca aaggcaatgc gcttatcttt gctggcaaac agatatgcat
    22801 ccccttcttc cgtctctgcc agaatttcca gatcggtgta atcacgaatc aacgctgccg
    22861 gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc atcaaagcca cgcgtcagaa
    22921 gcgcatgagg atggagaata tgatgctcgt aaacgccaga gagtttgtcg gtgcgagttt
    22981 gcttaggaat gccgtagagg atattgagcg cggcctgtac cgcccagcag acaaacagcg
    23041 tcgaggtgac gtgatctttc gaccactcca gcacctgttt gatctgcggc cagtaagcga
    23101 catcattaaa ctccaccagg cccagcggcg caccggttac aatcagaccg tcaaagttct
    23161 gttcctgaat atcttcaaag ttacagtaga agttgttcag atgctctgcg ggcgtgttgc
    23221 gcgattcacg ggaatcgatg cgcaacagct gaatatcgac ctgcaaaggt gagtttgaaa
    23281 gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg catcaggtta aggatcagaa
    23341 ccttaagtgg acgaatttcc tgaccagacg cacgagaagt tgtcatcaca aagacgtttt
    23401 cttcacgcaa gaaattgacg gcgggtagct cgtccggcac acgaatcggc ataacctgat
    23461 tacctcacta catacgctta tacgtttaga catccagata gctgaagata accagaaaat
    23521 ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt caaggaaaag gctacgttag
    23581 aatataagaa tgacgaaaag gagagaaaat ggttattagt attcgccgct cacggcatga
    23641 ggaaggggag gaactcgttg cgatttggtg tcgatctgtc gatgccaccc acgatttttt
    23701 atcagcagag tatcgggccg agctggaaga gctggtgcgt tcctttctgc cggaagcacc
    23761 gttgtgggtc gcggttaatg aacgggagca gccggttggg tttatgttgt taagtgggca
    23821 gcatatggat gcgctgttta tcgatcctga tgtgtgcggc tgcggcgtgg gtcggatgct
    23881 ggtggagcat gcgctctcga tggcaccgga actgacaacc aacgttaatg agcaaaatga
    23941 gcaggcggtt gggttctata agaaggtggg ttttaaggtt acgggacgct ctgaggtgga
    24001 cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat gtgggggaat aagccgcctc
    24061 tgccgtgcct gcccaagctg gagaagacg
//
LOCUS       DAJTSF010000062        23339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000062 DAJTSF010000000
VERSION     DAJTSF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.062"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..613
                     /gene="ushA"
                     /locus_tag="OO335_002538"
     CDS             <1..613
                     /gene="ushA"
                     /locus_tag="OO335_002538"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6002882.1"
                     /translation="LLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILA
                     AQMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTA
                     VAQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYP
                     RLDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(650..1129)
                     /gene="ybaK"
                     /locus_tag="OO335_002539"
     CDS             complement(650..1129)
                     /gene="ybaK"
                     /locus_tag="OO335_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6002883.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(1333..2127)
                     /locus_tag="OO335_002540"
     CDS             complement(1333..2127)
                     /locus_tag="OO335_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6002884.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            2265..2606
                     /locus_tag="OO335_002541"
     CDS             2265..2606
                     /locus_tag="OO335_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6002885.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(2821..5325)
                     /gene="copA"
                     /locus_tag="OO335_002542"
     CDS             complement(2821..5325)
                     /gene="copA"
                     /locus_tag="OO335_002542"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6002886.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            5587..6519
                     /gene="glsA"
                     /locus_tag="OO335_002543"
     CDS             5587..6519
                     /gene="glsA"
                     /locus_tag="OO335_002543"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6002887.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            6522..7814
                     /locus_tag="OO335_002544"
     CDS             6522..7814
                     /locus_tag="OO335_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6002888.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            7939..8346
                     /gene="cueR"
                     /locus_tag="OO335_002545"
     CDS             7939..8346
                     /gene="cueR"
                     /locus_tag="OO335_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6002889.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(8347..8805)
                     /locus_tag="OO335_002546"
     CDS             complement(8347..8805)
                     /locus_tag="OO335_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6002890.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(8802..9719)
                     /locus_tag="OO335_002547"
     CDS             complement(8802..9719)
                     /locus_tag="OO335_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6002891.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            9865..10542
                     /gene="fetA"
                     /locus_tag="OO335_002548"
     CDS             9865..10542
                     /gene="fetA"
                     /locus_tag="OO335_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6002892.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            10529..11308
                     /gene="fetB"
                     /locus_tag="OO335_002549"
     CDS             10529..11308
                     /gene="fetB"
                     /locus_tag="OO335_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6002893.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(11371..12225)
                     /gene="cnoX"
                     /locus_tag="OO335_002550"
     CDS             complement(11371..12225)
                     /gene="cnoX"
                     /locus_tag="OO335_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6002894.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(12286..13095)
                     /gene="ybbO"
                     /locus_tag="OO335_002551"
     CDS             complement(12286..13095)
                     /gene="ybbO"
                     /locus_tag="OO335_002551"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6002895.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(13085..13707)
                     /gene="tesA"
                     /locus_tag="OO335_002552"
                     /pseudo
     CDS             complement(13085..13707)
                     /gene="tesA"
                     /locus_tag="OO335_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            13678..14364
                     /locus_tag="OO335_002553"
     CDS             13678..14364
                     /locus_tag="OO335_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6002896.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            14361..16776
                     /locus_tag="OO335_002554"
                     /pseudo
     CDS             14361..16776
                     /locus_tag="OO335_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(17489..18583)
                     /gene="mnmH"
                     /locus_tag="OO335_002555"
     CDS             complement(17489..18583)
                     /gene="mnmH"
                     /locus_tag="OO335_002555"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6002897.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(18652..19578)
                     /gene="allS"
                     /locus_tag="OO335_002556"
     CDS             complement(18652..19578)
                     /gene="allS"
                     /locus_tag="OO335_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR6002898.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            19808..20290
                     /gene="allA"
                     /locus_tag="OO335_002557"
     CDS             19808..20290
                     /gene="allA"
                     /locus_tag="OO335_002557"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR6002899.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            20368..21183
                     /gene="allR"
                     /locus_tag="OO335_002558"
     CDS             20368..21183
                     /gene="allR"
                     /locus_tag="OO335_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR6002900.1"
                     /translation="MTEVRRRGRPGQAAPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            21273..23054
                     /gene="gcl"
                     /locus_tag="OO335_002559"
     CDS             21273..23054
                     /gene="gcl"
                     /locus_tag="OO335_002559"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR6002901.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            23067..>23339
                     /locus_tag="OO335_002560"
     CDS             23067..>23339
                     /locus_tag="OO335_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR6002902.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT         5316 a         6027 c         6308 g         5688 t
ORIGIN      
        1 gctgttatca ccgttccaga acaaaggcaa agcgcagctg gaagtgaaaa taggcaaaac
       61 caatggtcgt ctggaaggcg atcgtgacaa agtgcgtttt gtacagacca atatggggcg
      121 gttgattctg gcagcccaaa tggatcgcac tggtgccgac tttgcggtga tgagcggagg
      181 cggaattcgt gattctatcg aagcaggcga tatcagctat aaaaacgtgc tgaaagtgca
      241 gccattcggc aatgtggtgg tgtatgccga catgaccggt aaagaggtga ttgattacct
      301 gaccgccgtc gcgcagatga agccagattc aggtgcctac ccgcaatttg ccaacgttag
      361 ctttgtggcg aaagacggca aactgaacga ccttaaaatc aaaggcgaac cggtcgatcc
      421 ggcgaaaact taccgtatgg cgacattaaa cttcaatgcc accggcggtg atggatatcc
      481 gcgccttgat aacaaaccgg gctatgtgaa taccggcttt attgatgccg aagtgctgaa
      541 agcgtatatc cagaaaagct cgccgctgga tgtgagtgtt tatgaaccga aaggtgaggt
      601 gagctggcag taatccgaaa gtgccggatg tttgcatccg gcacaatgct taatcgcggc
      661 gggcgatatc agcaaatttg gcatcgagga tctttgccag atcgcctgcc gccagttcga
      721 tatccagtcc gcgcttgccg ccggaaacat aaatagtggc aaattcttgt gcgggggcgt
      781 cgataatcgt tggcagacgt tttttctgcc ccagtgggct aatcccccca accaggtatc
      841 ccgtcgaacg ctgcgcgacc atcggatcgg ccatctcaac tttcttggca cccagcgctt
      901 ttgctacttt tttaagatcc agttgacctg cgaccggcgt aacggccacg gcaaggtgtt
      961 tcatatcacc gttcactgcc accagcagcg ttttgtagac ctgatccgga ttcaaaccta
     1021 attttttgac gacttcatcg ccaaaattgg tttcagccgg atcgtgctcg taggtatgga
     1081 tttgaaacga aatcttgttt ttttcgagta atttaactgc gggtgtcatg gtaattttcc
     1141 tgccttaaac aaaaaaacga agcaagcata cgcctgatgg cggtccaaaa aaaagagtca
     1201 tcttgcctaa gagtattggc aggatggtga gattgagcga caatcgagtt acaccgtcgc
     1261 ttaaagtgac ggcataataa taaaaaaatg aaattcctct ttgacgggcc aatagcgata
     1321 ttggccattt ttttagcgca acatttgcgg caaattccct tctccataca ggtgtagtgc
     1381 accgaccgcg accacatatc gccccggcgg catggcgcgt aatttatccc gccaggcgag
     1441 atttcgctga tgcatcagca catcgtacag cgactgactg aacgtattgg gcagcgttat
     1501 atcattattt tgcggcggtg cattcagcca ccagctcatc atttgttgca gcaaccgtgc
     1561 gttggtatgc cagtgggtca gcgtatcgtc cagcagcgcc agtcctttgt cagggagctg
     1621 gagcaacatg gcaatctggt tttcagcccc ttccagttca atcacgggtt tatgttgttg
     1681 cttcgccgcc tgcaatagct ggtaatcgat accgtattct gcccgcagcc ccagtttttg
     1741 tgcctgcgtc gcctgaagaa ccatcgcgat ttgccacagc ggttgggtag aaaagagtga
     1801 gggagaaatg cccatctcct ggctaatgtg ctgcaggttt tggagttgct cctcgctaat
     1861 gcgctcttcc agcgcctcgc aggcaggcaa attagcaaaa ggcgtatcgc tggtggaaac
     1921 atccgcctcg acgatcagcg catcggcgtt tttgagcttt ttgagcaaac gggtgggcag
     1981 gggagccata tcgtggctac ccatatgaat actgccaatc agatgaaaat ggcgattgcc
     2041 ggggagggtg atatcgatgg caggccaggt gtaatgatta ccgcgcagcg cggcccaaag
     2101 cgtttttacc cggtacaaca gatccatacg acctcccttt gtgaaatatc atgctagcgc
     2161 gcggtgaggg atggcgcaaa tggagttggg gacttgttta atgtgtttgt atgattcagt
     2221 atgttcttgc atcgctattc acagggaagc aacagttaaa aaccatgaaa caggcaacaa
     2281 gaaaaccgac gacacccgga gatattcttc tctatgaata tctggaaccg ctcgatttga
     2341 aaatcaatga gttagcagag ttgctgcatg ttcatcgtaa tcgcgtcagt gcactgatca
     2401 ataacaatcg taaactcacc actgagatgg cattccgtct ggcgaaagtt tttgatacca
     2461 cagtcgattt taggctaaac ctccaggcgg cagttgatct ttgggaagtt gaaaacaaca
     2521 tgcgcaccca ggaagaattg ggacggattg aaacagtggc tgaatatttg gcacgccgtg
     2581 aagagcgtgc aaaaaaggtc gcgtaaggcc aaaacatcgc ttcatctcgt aggccggata
     2641 aggcgttcac gccgcatccg gcatccgagc accattgcct gatgcgacgc ttacgcgtct
     2701 tatcaggcct acaaacctgt gcctgaaccg taggccggat aaggcgttta cgccgcatcc
     2761 ggcagtcatg cgtcgatgcc aaatgcgccg ccctaaagca gcgcatccgc aatgacgtac
     2821 ttattccttc ggtttaaacc gcagcaaccg gttggcgtta cttactacgg taattgacga
     2881 gagcgccatt gccgctccgg caactaccgg gttaagcagt gttccagtaa acggccacaa
     2941 aataccggcg gcgaccggaa taccgatgct gttgtagata aaggcaccga gcaggttctg
     3001 cttcatgttg cgcagcgttg cgcgggaaat cgcgagcgca tccgcaacgc ccatcaggct
     3061 atggcgcatc agggtaatcg ccgcggtttc aatggcaaca tcactgccgc cacccatcgc
     3121 aatgccgaca tccgcctggg ccagcgctgg cgcgtcgtta atgccgtcgc ccaccattgc
     3181 cacctgacgt ccttcacttt gcagacgttt gatcgcttcg gctttaccgt ccggcagcac
     3241 tccggcaatc acttcatcaa ttccagcttc tttggcgatc gcattggcgg tggttgggtt
     3301 atccccggtc aacatcacca gacgatatcc cgctttatgc aggcgttgca gcgccgccac
     3361 gctatcacta cgcaacggat cgcgtacagc cagcagggct actgctttcc cgtcaatcgc
     3421 cagcagcaca ggcgttgctc cttgcgatgc ctgagctgaa atatcggctt caatcgcttt
     3481 agtatcgact tgctgatcat ttaacaacgc ctgattaccc agcaataacg catgaccttc
     3541 tgcttcaccg ctcacgccca gcccgcgcaa tgtgcggaaa ccgttgacct gcggtagctg
     3601 catatcgcct gctttatcaa ggatcgctcg tgccaatgga tggctggaac cttgctccag
     3661 cgccgccgcc agacgcagcg cctgcgcttc gtcaacatca gcaaatgttt tcactgcgac
     3721 aacctgcggc ttcccttcgg tcagcgtccc ggttttatcg aacacgacgg tatcgagtgt
     3781 actggcgcgt tgcagcgcgt cggcgtcccg caccagcacg ccaaactcag ccgcccgccc
     3841 gacgccggaa ataatcgaca tcggcgtcgc cagtcccagc gcacacgggc aggcgataat
     3901 cagcaccgta gtggcaatca ccagggtata gacaatctgc ggtgccggac caaagaaata
     3961 ccagattgcc gcactgacaa gcgcaataac cactactacc ggcacaaata tggctgagat
     4021 tttatccgcc agctgaccga tttctggctt gctgctctgg gcctggcgca ccatgcgaat
     4081 gatccttgat agcgtggtat ggctgccaac cgcactggca cgaaacagca cactgccgtc
     4141 ctgtaccact gtcccggcat ggacgctatc gccttcgcct ttttgctgcg ggattggttc
     4201 gcccgtcagc atcgcttcat ccagccatgc ttcgccctgg gtaatctcgc catctaccgg
     4261 cacgcgatcg ccggtcgtca ggcgcagcaa catacctggc tgcacttctg ccagaggcac
     4321 gcttttttca ccttcgtcag taaccaggcg cgccgtcggt ggggttaaat cgagtaattt
     4381 ctccagtgcc ttagaagaac gctgacgtgc gcgtgcttcc agcatatggc cgagattgat
     4441 cagaccgata atcatcgcac tggcttcata gtaaagatgt cgcgcttcca tcgggaacca
     4501 ctgcggccac aggttgacgc tcatcgaata gagccacgcc acgcccgttc ccagcgccac
     4561 cagcgtatcc atcgtcgctg caccgttcag caggcttttc catgcactgc ggtaaaaatg
     4621 gccgctggcg aaaaccatca ccgccagggt tatcaggccg ataaccaacc acaggctgcg
     4681 gttgtcggcg gtgactatca tgttatcgcc gatcatcccc cagaccatca ccgggatacc
     4741 caccgccagt gcgacaattg cctgccagcg gaagcgcttc atcgtagcga cggcggtttc
     4801 ttgctggcgt tcgcggcgtt tcgcgtcatc ttcaatcgct tccgcgccgt agcccgcttt
     4861 ttccaccgcc tgcactaaat cttgtgggga ggcactgccc atcaccagcg cagtacgctc
     4921 cgccaggttt acccgtgcct gagtgacgcc cggtacgctt tgcagcgcat tttgtacgcg
     4981 ggtgacacag ctggcgcagc tcatgccgct cagcagcaac tgctggctgt catcgtcatc
     5041 ggcggtcgct gccggaagcg cctcagaaac cgctgtcagt gcttccgacg ggattgatga
     5101 ctccgccagc ggtttagcct ttgggtggct tacagatgcg tcataacccg cttgtttgat
     5161 ggtttcgatc agctgttctg cactggcagt cccggtaacg tgcgcttcag tgatagacac
     5221 atccgcttgt tccacatccg ggcgctgttc aagactttct ttcacgcgtt taacgcagtg
     5281 accgcaggac aggccgtcca ggttcaggtc gatagtttgt gacataaaac actcctttaa
     5341 gacagttttg actggctgtg ataaaggtta aaccttccag caaggggaag gtcaagaaat
     5401 taataaacca ggcgggtaaa agtccgtaaa gattaaaaaa tcggctcgat ttgcatcagg
     5461 attagacatt tatttctttg ttttcctgta gttaagttgc gggtgctaag ttaaatcagg
     5521 atgcctgaaa atcggcactg gggtgaggaa ttacctcccg catctataaa aaggagttaa
     5581 caaaagatgt tagatgcaaa caaattacag caggcagtgg atcaggctta cacccaattt
     5641 cactcactta acggcggaca aaatgccgat tacattccct ttctggcgaa tgtaccaggt
     5701 caactggcgg cagtggctat cgtgacctgc gatggcaacg tctatagtgc gggtgacagt
     5761 gattaccgct ttgcactgga atccatctct aaagtctgta cgttagccct tgcgttagaa
     5821 gatgtcggcc cgcaggcggt acaggacaaa attggcgctg acccgaccgg attgcccttt
     5881 aactcagtta tcgccttaga gttgcatggc ggcaaaccgc tttcgccact ggtaaatgct
     5941 ggcgctattg ccaccaccag cctgattaac gctgaaaacg ccgaacaacg ctggcagcga
     6001 attttacata tccaacagca actggctggt gagcaggtag cgctctctga cgaagtcaac
     6061 cagtcggaac aaacaaccaa cttccataat cgggccattg cctggctgct gtactccgcc
     6121 ggatatctct attgtgatgc aatggaagcc tgtgacgtgt acacccgtca gtgctctacg
     6181 ctcatcaata ctgttgaact ggcaacgctt ggcgcgacgc tggcggcggg tggtgtgaat
     6241 ccgttgacgc ataaacgcgt tcttcaggcc gacaacgtgc cgtacattct ggccgaaatg
     6301 atgatggaag ggctgtatgg tcgctccggt gactgggcgt atcgcgttgg tttaccgggc
     6361 aaaagcggtg taggtggcgg tattctggcg gtcgtccctg gcgtgatggg aattgccgcg
     6421 ttctcaccac cgctggacga agaaggcaat agtgttcgcg gtcaaaaaat ggtggcatcg
     6481 gtcgctaagc aactcggcta taacgtgttt aagggctgat catgatgaac acggaaggta
     6541 ataacggtaa caaacccctc ggtctatgga acgtcgtttc catcggtatt ggggcaatgg
     6601 tgggggcggg gatcttcgcg ctgctggggc aggctgcgtt gctaatggaa gcctcgacct
     6661 ggggcgcctt tgcttttggc ggtattgtgg ctatgttttc cggttatgcc tatgcgcgtc
     6721 tgggggcgag ctatcccagc aatggcggta ttatcgactt ttttcgtcgc ggattaggca
     6781 acggcgtctt ttcgctagcg ctctcgttgc tatacctgtt gacgctggcg gtgagcatcg
     6841 ccatggttgc ccgtgctttt ggcgcttatg ccgtgcagtt tttgcatgaa ggcagccagg
     6901 aggagcacct tattttgctc tacgcgttgg ggatcattgc ggtgatgacg cttttcaact
     6961 ccttaagcaa ccatgcggta gggcggctgg aagtgatcct cgtcggcatt aaaatgatga
     7021 tcctgttatt gctgattatt gccggtgtct ggtcgctgca accggcgcat atttccgtct
     7081 ctgcgccccc cagctccggt gcgttcttct cctgtattgg gataaccttc cttgcctatg
     7141 cgggctttgg catgatggcg aacgcggcgg ataaagtgaa agatccgcag gtcattatgc
     7201 cacgggcgtt tctggtggcg attggcgtta ccacgttgct ttatatctcg ctggcgctgg
     7261 ttttgcttag cgatgtatcg gcattagagt tagaaaaata tgctgatacc gccgtagcgc
     7321 aggctgcttc tccgctgctc gggcatgtgg gttatgtgat cgtcgtcatc ggcgctttac
     7381 tggcgacggc ttcaggcatt aacgcgaacc tgttcgccgt gtttaacatc atggacaaca
     7441 tgggcagcga acgcgaactg ccgaagctaa tgaataaatc cctgtggcgg cagagtacct
     7501 ggggcaatat catcgtcgtg gtgttgataa tgctgatgac ggcggcgcta aatttagact
     7561 cactcgccag cgttgccagc gccacctttt tgatctgcta tctggcggtg tttgtggtgg
     7621 cgatccgcct gcgtcatgat attcacgcct cgttgccgat tcttatcgtt ggtacgttgg
     7681 tgatgttgtt ggtgatcgtt ggctttatct acagcctgtg gtcccagggc agccgtgcgt
     7741 tgatatggat tattggtgca atcttactca gccttattgt ggcaatggtc atgaagcgta
     7801 ataaaaccgt ataacacctc tctgtgcgca gtacttcctg tattattgtg gtggcggtcg
     7861 atattcgcac tggcaaaaaa acgtgcttga atatctgttg aaacccttta acaaagcaca
     7921 ggaggcgttg cgcgaacgat gaacatcagc gatgtagcaa aaattaccgg cctgaccagc
     7981 aaagccattc gcttctatga agagaagggg ctggtgacgc cgccgatgcg cagcgaaaac
     8041 ggttatcgca cctacacgca gcagcatctc aacgaactga ccttactgcg ccaggcacgg
     8101 caggtgggct ttaacctgga agagagcggc gagctggtga atctgtttaa cgacccgcag
     8161 cggcacagcg ccgacgtcaa acggcgcacg ctggagaagg tggcggagat cgaacgacac
     8221 attgaggagc tgcaatccat gcgcgaccag ctgctggcac tggcgaatgc ctgccctggc
     8281 gatgacagcg ccgactgccc gattatcgaa aatctctccg gctgctgtca tcatcgggca
     8341 gggtgattaa gacgagacgg ctcggatatg tagggttatc ccttctatcg caatgacttc
     8401 aacatgcgta cctgcaccga gatcctcgct ggcgctgaca ggccatgaac tgtcaccgac
     8461 gcgcatatga ccgcgcccgt tgaccagcgg agattccagc acaaaacgtc ggccaatcag
     8521 ctgctgcccg cgctggttta aatgactatc gctgtgcttt tgttcgcgca cccgccgcga
     8581 caaccatttc caccacagcc aggcggcgag cagcgtcagg atggcaaaca ttaccccttg
     8641 ccactcccaa cccagcggca ccagccagac caccaggccg gtaatcaccg ctgccacgcc
     8701 gctccacaac aaataaccat ttccgcccag catctcggct gccagcagca acccgccgag
     8761 actgagccag aaaatatgtg gatgaacgac cattaactcc atcatggctg agtccgtttg
     8821 ttggcgctgt ctttcaccag ctcggcaatc ccggcaatcg accccatcag gctgctggcc
     8881 tctaatggca tcatcactac tttgctgtta ctggaggaac cgatctgctg taacgcttcg
     8941 gtgtattttt gcgctacgaa gtagttcacc gcctgaatat caccggaggc gatagcttca
     9001 gacaccattt tggtggcgcg ggcttctgct tcggcggaac gttcacgcgc ttcagcctgt
     9061 aaaaacgccg actgacgttc gccttccgct ttcaggattt gcgactgttt ttcaccttcg
     9121 gctttgagga tttccgcctg acggatccct tccgcttcaa gaatgtaagc gcgtttggta
     9181 cgttccgctt tcatctgcgc gttcattgaa gagataagct ctgccggtgg gcgcacgtcg
     9241 cgaatttcaa tacgggtgac tttaatcccc cacgggttgg tggcttcatc gacaatacgc
     9301 agcaggcgtg agttgatgct gtcgcgctga gagagcattt cgtcaagttc cattgaaccc
     9361 aacacggtac ggatgttagt catggtcagg ttgatgatcg ccagctccag attgctgact
     9421 tcataggcag cgcgtggcgc gtcaatcacc tgaataaaac agactgcgtc gatggtaacg
     9481 ttggcgttat ctttcgagat aacttcctgg gaagggatat cgagcacttg ctccatcata
     9541 ttgatcttgc gaccaatgcg atccataaac ggcaccacca gactgagccc cggctgtaac
     9601 gttttggtat agcgaccaaa acgttctacc gtccattgat agccctgcgg cacgattttg
     9661 acgcccgcgc cgacaatgac cagcgcgaca aaaatgagaa tcgggataaa gataagcatc
     9721 ggaaaaacct cctgttgtac cgtccataat cagcaaaatt gctgcttgat taaacaaatt
     9781 atacctgatt actgaaagag agttccccct tattcctgcg aaggataaac tgtttttagt
     9841 aaaaatcaga aaaagggaac agcgatgcag gaaaatagtc ctttgcttca gctacaaaac
     9901 gtaggatatc tggcgggtga tacgaagatt cttaataaca tcaatttttc gctgcgtgct
     9961 ggcgaattta agttaattac cggtccttct ggttgtggca aaagtacgct gctaaaaata
    10021 gttgcttcat tgatcagccc aaccagcgga acgttactgt ttgaaggtga ggatgtcagc
    10081 acactaaaac cagaaatcta ccgccagcaa gtctcttact gcgcccagac gccgacgctg
    10141 tttggcgata cggtatacga taatctgatc tttccctggc agatccgtga ccagcagcct
    10201 gacccggcca tttttctcga ttttctcgaa cgcttcgctt tgccggacag cattttgacg
    10261 aagaatatcg ccgagctatc tggtggtgaa aaacaacgca tctcattgat tcgtaacctg
    10321 caatttatgc cgaaggtttt attgctggat gaaataacca gtgcgctgga tgaaagtaat
    10381 aaacataacg tcaatgagat gatccatcgt tatgtgcgcg agcaaaatat tgccgtgctg
    10441 tgggtgacac acgataaaga cgaaattaat catgcggata aagtgattac actgcaaccg
    10501 catgccggag aaatgcagga agcacgctat gaactcgcat aatattacta acgaatcatt
    10561 agcactggca ttaatgctgg tggtggtggc aatcttaatt agccataaag aaaaactggc
    10621 gctggagaaa gatattctat ggagcgtcgg gcgagcgata attcagctga ttattgtcgg
    10681 ctatgtgctg aagtatattt tcagcgtgga tgatgccagc ctgacattat tgatggtgtt
    10741 atttatctgc tttaatgcgg cgtggaacgc gcaaaaacgc agtaaatata ttgctaaagc
    10801 ttttatctca tcgtttattg ccattacggt cggggcggga attaccctgg cggtgctgat
    10861 tctctcaggg gcgattgaat ttatcccgat gcaggtgatc cctgtcgccg ggatgattgc
    10921 cggtaacgcc atggtagcgg tggggttgtg ttacaacaat ttagggcaac gggtcattag
    10981 cgaacagcaa cagattcagg agaaactgag tcttggcgcg acgccgaagc aggcttcagc
    11041 gatattgatt cgcgacagta ttcgcgcggc tttaattccg acggtcgatt cagcaaaaac
    11101 ggttggctta gtgagtttac caggaatgat gtccgggctg atatttgccg ggattgatcc
    11161 ggtgaaggcg attaaatatc agattatggt gacctttatg ctgctctcaa ccgccagctt
    11221 gtcgaccatt attgcctgct atttaaccta tcgtaagttt tataattcgc gccaccagtt
    11281 ggtggtgact caattgaaga agaaatgatg cgatgccgga tgcgcagcat cgcatccggc
    11341 gttgtggttc atgtgccgaa caacggaata tcaatacaac aatgcataca gctggcggcg
    11401 atacttcgac gccagtgcat cacccgtacc cagcgcagcg aggatctcct ggaacgtttt
    11461 acgcgtctga ccgtctgcgg cggtgagatc tttacgcaga tgcccgaaca gcaactccag
    11521 cgcctcttca ttgcgcccaa cctgatgcag ttgcagcgcc agttgcgtcg ccagtgcggc
    11581 atcttctgga ttctccgcca cctgctgttg caactgttga atttccggct tatcagccgc
    11641 ctgcttcagc agttcgattt gcgctaccag cccctggtag cgggtgtcct gatcctgcaa
    11701 cggaatggtt ttcagcaccg cttccgcatc ttcagaacgg ttcagcgcaa tcagcgtttc
    11761 tgccagcagc aggccgatct ccccgttctg attcgacaac tgccaggcgt ctttcagcaa
    11821 tggcagggca tcggtgtaat tgccttcctg catcagttgc atcgcctgct gcgctttcag
    11881 ctcttcttcg cgcggcagca ctttatccag cagggcgcgg atcgcctctt ccggttgcgg
    11941 cccctggaag ccatctaccg gttgcccgtt ctggaacaga tacacggtcg gaatcgcgcg
    12001 cagaccaaac tgggcggcaa tcatctgctc cgcgtcgcag tccagcttcg ccagaataaa
    12061 ttgcccgttg tactgcgccg cgaggctttc caggatcggg gttaactgca aacagtgctg
    12121 gctacgttca gaccaaaaat agaacagcac cggagtggtc atcgactgtt caagaacctg
    12181 ttgcaggtta gattcgttaa tgttgacaat attttctacg gacatggagt cgctctctgt
    12241 tgtcgatatt ttctttgaca tgggggctta agcgcgcgct tcaactcacc cctgcaatat
    12301 tttgtccatc acgcgcccag gcagcaggcg ctcgagcacc attactgccc aggtcaccag
    12361 cgttaccgga tagcgcatct tcggcttctc gctaataaaa gcatggcgca ctttgtcgac
    12421 caccgcttcc ggtcccaacg taaagcgggc ggcgatgccg ggattttcga ctggtttatc
    12481 actttgcgtc tggttgacgt tgtcggtgaa gcgagtacga atgggaccgg gttcgatcag
    12541 gctgacttta attccgctgt ggcgtagctc catgcgcagc gcgtctgacc acgcctccag
    12601 cgcatattta ctggccgcgt aagcgccacg acccggcgtg gagattaatc ccatcaccga
    12661 tgatgtcatc acaatacgcc cttcaccgtg cggtaacatc gcgggtaaca ggcgcatggt
    12721 gagctggtgt gcgccgaaaa agttggcgga aaactgctgt tccatctgcg cacggctgat
    12781 ggtggaaagg gggccataca tgccgaatcc ggcattgtta aagatcccat acagacaatt
    12841 atcggtcagg gcgatcacct cgtctgctgc gcgattaaca ctttctggtg aatccagatc
    12901 gatcaacacg ccggtaaatc ccatgttgtt catacgctca acatcattcg gttttcggca
    12961 acctgccagc acatgaaaac cctggcgttt taattcgagc gcgctttcca ggccaattcc
    13021 actggaacat ccggtaatta agaccgattt ttgcataact ttacctgtca ggatctccgt
    13081 tgctttatga gtcatgattt actaaaggct gcaactgctt cgccatccag tcggcaataa
    13141 acggctgggc gtcgcggttg ggatgaatac cgtcatcctg catccattgt ggcttgaggt
    13201 agacctcttc cataaaaaag ggcagcagcg gaacatcaaa ctctttggcg agtttggggt
    13261 aaatggcgct aaaggcttca ttataacggc gaccatagtt tgcaggcaga cgtatttgca
    13321 ttaacaatgg ttcagcgttg gcggctttga catcctgcaa aatctggcgc agcgtttgct
    13381 cggtttgctg tggctgaaaa ccacgcaaac catcattgcg cccagttcaa ccagcaccca
    13441 acgcggctga tgctgtttga gcagagccgg aaggcgcgcc agcccttgtt gcgaggtgtc
    13501 gccgctgatg ctggcattaa ctaccgacgt tttactctgc cacttatcat tcaacaaggc
    13561 aggccaggcc gcgctggcag acattcgata cccggcgctc aggctatcac ccagaatcaa
    13621 taacgtgtcc gctgcggcgg cacggaaggt taacaggacc aggaacagga agggcaaatg
    13681 ccagcggaaa acattgttga agttcatcat cttaagaagt ccgtcggtca gggggagcat
    13741 gaactctcca tcctcaccgg agttgagctg gttgtcaaac gtggcgagac catcgcactg
    13801 gtgggcgagt cgggatcggg taagtcaacc ttgctggcga tcctcgccgg gcttgatgac
    13861 ggcagcagtg gcgaagtgag tctggtggga caaccgctac ataatatgga cgaagaagcg
    13921 cgggcaaagt tgcgcgcgaa gcacgtcggc tttgtttttc agtcatttat gttaattcct
    13981 acccttaacg cgctggaaaa cgtcgagctt ccggctctgc tgcgcggtga gagtagcgcg
    14041 gaaagtcgta acggggcgaa agcgttgctc gaacagttag ggctgggtaa acgtctggat
    14101 catcttccgg cacagctttc cggcggtgaa cagcaacgag tggcgctggc acgagccttt
    14161 aatggtcgac ctgatgtgct gtttgccgac gaacccaccg gcaaccttga ccgccagacg
    14221 ggcgataaaa ttgccgacct gctgttttcc ctcaaccgtg aacatggcac cacgttgatt
    14281 atggtgaccc acgacctgca actggcggca cgctgcgacc gctgcttacg gctggtgaac
    14341 gggcagttgc aggaggaagc atgattgcac gttggttctg gcgcgaatgg cgttcgccgt
    14401 cgctattaat tgtctggctg gcgctaagcc tggcggtggc ctgcgtgctg gcgctgggca
    14461 atatcagcga tcgcatggag aagggcttaa gccagcaaag ccgtgagttt atggcgggcg
    14521 atcgggcgtt gcgcagttca cgcgaagtgc cgcaagcgtg gctggaggaa gcgcaaaagc
    14581 gcggcctgaa agtcggcaag cagctgactt tcgccacaat gacctttgca ggcgacacac
    14641 cgcagctggc gaacgtcaaa gcggtggatg atatctaccc gatgtatggc ggtctgcaaa
    14701 ctaatccccc tggcctgaaa ccgcaggcgg gcagcgtatt gctggcccca cgcctgatgg
    14761 cactgcttaa cctgaaaacg ggcgacacca ttgacgtggg cgatgccacc ttgcggattg
    14821 ccggagaagt gattcaggaa ccggattccg gttttaaccc cttccagatg gctccgcgtc
    14881 tgatgatgaa tctggcggat gtcgataaaa ccggagccgt gcagccgggg agtcgggtca
    14941 cctggcgtta taaattcggc ggcaacgaga accagctcga cggctatgag aaatggttgt
    15001 tacctcagct taaacccgaa caacgctggt acggtctgga acaggacgaa ggcgcgctgg
    15061 ggcgatcgat ggaacgctcg caacagttcc tgctgctttc ggcgcttctg accttgctgc
    15121 tggcagtggc agcggtggcg gtagcgatga atcattactg tcgcagtcgc tacgatctgg
    15181 tggcgatcct caaaacgctg ggggcagggc gagcgcaact gcgtaagcta atcgtcggtc
    15241 agtggttgat ggtgctgacg ctttcagccg ttaccggtgg ggccataggc ctgttgttcg
    15301 aaaacgtgtt gatggtgctg ctcaagcccg ttctgcctgc tgcactaccg ccagccagcc
    15361 tctggccgtg gctgtgggcg cttggcacca tgacggtcat ctcgctgctg gtggggctac
    15421 gaccataccg cttgttgctg gcaacgcagc ctttacgcgt attacgtaat gatgtggtcg
    15481 cgaacgtctg gccgctgaag ttttatctgc cgattgtcag tgtggtggtt gtgctgctgc
    15541 tcgccggatt aatgggtggc agcatgctgc tttgggcggt gctggcgggc gcggtagtac
    15601 tggctttgct gtgcggtgtg ctgggctgga tgctgctgaa tgtacttcgc cgcatgacgc
    15661 tgaaatcgct gcctctgcgc ctggcggtta gccgcctgtt acgtcagccg tggtcaacgt
    15721 taagccagct ttcggcattt tcgctctcct ttatgctgct ggcactgctg ctggtgctgc
    15781 gtggcgatct gctcgaccgc tggcagcagc agctaccgcc agaaagcccg aactactttt
    15841 taattaacat cgccacagaa caggtagcac cgctaaaagc gttcctcgcg gaacatcata
    15901 tagtcccgga atcattttat ccggtagtgc gggcgcggct gacggcgatt aatgataagc
    15961 cgacagaagg taatgaagat gaggcgctta atcgcgaact caatcttacc tggcaaaata
    16021 cgcggcccga tcataatccg attgtcgccg gtaactggcc gccaaaagcc gatgaagtgt
    16081 cgatggaaga ggggctggcg aaacgcttaa acgttgccct cggtgatacc gtgactttta
    16141 tgggcgatac ccaggagttc cgcgctaaag tgaccagcct gcgcaaagtg gactgggaaa
    16201 gtctgcggcc taatttctat tttattttcc ctgaaggggc attagacggg caaccgcaga
    16261 gctggcttac cagtttccgc tgggagaatg gcaacggcat gttgacgcaa ctcaaccgcc
    16321 agttcccgac cattagcctg ttagacattg gcgcgatttt aaaacaggtc ggtcaggtgc
    16381 tggagcaggt aagtcgggcg ctggaggtga tggtggtgct ggtcaccgcc tgcggtatgt
    16441 tgctgttgct ggcacaggtg caggtgggaa tgcgccagcg tcatcaggag ctggtggtat
    16501 ggcgcacact cggtgcgggg aaaaaactgc tgcgcacgac attgtggtgt gatgttcgcc
    16561 atgcttgggt ttgtttccgg cctggtggcc gcaattggtg cggaaacggc actggcagtg
    16621 ttgcaggcga aagtgtttga tttcccgtgg gagccagact ggcgattgtg gattgttctg
    16681 ccgtgcagcg gtgcgctgct gctgtcgctt ttcggcggct ggctgggtgc gcgactggtt
    16741 aagggtaagg cgctgttcag gcagtttgtg gggtgatgac aggtgttaga ttgcctgatg
    16801 cgctacgctt atcaggccta cgaagagcgt gcaatatatt gattttgcaa gattgtgtag
    16861 gccggataag gcgtttacgc cgcatctggc atgaacagtg tgcattttgg caataaatat
    16921 gaaaccaact gtcatgtcgg tttttttaaa ggccgaagga ggtaaacctt cggccttagc
    16981 tacgtttcct cagttattca ataaactgac ggatcagttc cagcagcgcg attagcgcct
    17041 taagaacgat gagaacttta tcgagaaaag ttttcatccg ttgctccctt aagggagaaa
    17101 gacaatcaca gcttaccctt acgttgccta taactatgat tttcttcatg tgctagatga
    17161 ggactgtctc tctccaaatt agagcaccag aatcagcacc acctgtatcc tgaccaggac
    17221 ttaaaagtac ttttccgcac tgcggtaatc ccttgaggca gtatcactac tagcgtcggg
    17281 attgtatcgc caacatagct gtttgaatat gtaggcatcc tgacttataa ttcacttgct
    17341 agatgaggac tgtttaattt ctgcccggaa ccaccaagtc ttgggcggaa aaaataaaaa
    17401 aaccgactgt catgtcggtt tttttatatc tgtattctgg tgcaaccatt ttgttctcac
    17461 gacaatcgcc gtttcagaat accgcccatc actgcgcctt aacccatacc gccacttccg
    17521 cccactcacc gcgaaagaca actttttccg cttttttctc aagctgatag cgatacattg
    17581 ggtcgtaata ttctttaagt aacggcacca gccaggccag atgaccgtcg gtgctgccgg
    17641 tggtgagttg cgttgtcagc gccgtatcca gctgggcagc gagttcgtta tagcgctgta
    17701 accccagccg acgcttaatc gccgaaagtc cgtgatgcag gtattcgcaa tattcctgcc
    17761 agccctgttc gtcgccataa gcgtgggtaa aatcatgatg catacgcaag aaatactctt
    17821 cattcaggcg ttcaagacgg atctcaaacg gatcttctac caccgcaatc gccgcctgag
    17881 tcattcgctc gcgcagacat tccggcaggt gattcgaacc gatcatccgg ctttcatctt
    17941 ccagcaccca caggcgcaaa tcctgacggg cgtcggtttt tagcatttcg gcagccagca
    18001 ggttttcaaa actcgcctgg ctaagttgtg gttgtaacgt gcgaccaaac gccgaaccgc
    18061 gatgacgcgc cagcccttcc agatcaacac cgtttggctg ttgttgcact aatagcgttt
    18121 taccgctgcc ggtacaaccg ccaatcagca ctatcggttt ttgtgccagt tcaatggtcg
    18181 cctgaatcgc ggtctggcgc agtgccttat aaccgccttc caccagcgga taatcaatcc
    18241 ccgcttcatg cagccagcgt tgcacaatat gtgaacgttg accgccacgg gcgcagcaga
    18301 gaataccttg cggattttgc aggcacgctg cccgccaggc gtacatacgc tgctgacgaa
    18361 tttcacccgc caccagttta tgccccagcg ccagcgctgc gtctgagcct tgctgtttat
    18421 agcaggtgcc aacggaggcg cgttcatcgt tatccattaa cggcagattg atagcggcgg
    18481 gcattgcgcc ttgctcaaac tcgatagggg cgcgaacatc aataatgggc gtatcagcaa
    18541 tcagcagggc acgatagtcc tgttccgtgt gtctctcttg catagttaaa agtgaacctc
    18601 aaatcagctt gcgcgctatt ttacgcgcca acgcgcaagg aaacttgatt tttaactgcg
    18661 tggggtgccg aaaatttcta aaaatccgct gatttccggc ctgtgttggg taaacaaggt
    18721 cacaatatct tctaccgctt tgcagctgcc aaatttgcgc catgccagac tcaatggcga
    18781 aggagggcgc atcgttggga ttacccggct gaccagttgt tgattatcga tcattgactg
    18841 gcaaagtgat tttggcaaaa aaccaatgcc aacacccgcc agatgggcgg cgattttcgt
    18901 ttccatatcg ggaacaataa tctctttttg ccccggcaat cgccaggcga cgcgtttggt
    18961 taaggtgccg gcgctgtctt caatattgac cgccggaaag cgacgcaact gcgcttctgt
    19021 tagcggctct tcaacgttcg ccagcggatg atccgccgcc atgacaaatc gccattgcac
    19081 cgatcctaag ggatcaagac taaaggtatt tgccagcgcc tcagttcccg tgaccccgat
    19141 agccagcgaa aaaccttcgt acaatagcga gtcccagacg cccatataga tttgtcggga
    19201 gatgtgaaac tgggtaaagg ggtaacgctc attcagccac gccagcaact gggcgacggc
    19261 ctgggggtta tagagcaggt tgttgatgac aatattcacc tggcgttcca cgccatcatt
    19321 cacctgttgc agctcgctgg gcatactttc cagccagctc agccagtctc tggcctggga
    19381 aagtagatgc tcgccagccg ctgtcaacgt tacgctacga gtcgtacgga aaaacagcgc
    19441 tactccggta ttctcttcca gaagtttaat gcgataactg atcgtcgccg tggttttaca
    19501 taatcgttct gccgcttttg aaaaacttcc tgtttcagca accgcaatga aagtccgcaa
    19561 ggtttctgga tcgaacatct tcaggtatcc ccttttaaat ccgcaagttg cgtgattttc
    19621 ttatcctctg atttatcagt atttttacat gataatcctg ttcaatttgt ggactaaatc
    19681 tagttttgga aaaacattcc aacttttgta ttaatgttgt tctcttaagg ttttagattg
    19741 cctgttattg aaaccaagct gaccggtcgg cggtggttga acggaattat gttacaagga
    19801 caaaaagatg aaacttcagg tattaccgtt aagtcaggaa gcctttagtg cttatggcga
    19861 cgtaatcgaa acgcagcaac gggatttttt ccatattaac aatggcctgg tggagcgtta
    19921 ccacgatttg gcgctggttg agattcttga gcaagaccgt acgcttatca gcattaaccg
    19981 cgcgcaaccg gcgaatctgc cgctgaccat tcacgaactc gaacgtcatc cgctgggcat
    20041 tcaggccttt atcccaatga aaggtgaagt gtttgtggtg gtcgtggcgt taggcgacga
    20101 caaaccagac ctgtcaacgc tgcgggcgtt tatcaccaac ggtgaacagg gggtgaatta
    20161 ccatcgtaac gtctggcatc acccactttt cgcctggcag cgcgtcaccg attttctgac
    20221 catcgatcgc ggcggcagtg acaactgtga tgttgaaagt attcctgaac aggaactctg
    20281 ttttgcgtga cgcctgcaac cgacttgcat aagataaact aattgttcat tgtttatgct
    20341 cacttgtagg tcggagttaa cgtaggtatg acggaagtta gacggcgcgg caggccagga
    20401 caggcggcgc ctgtggcaca gaagggcgca caggcgttag agcggggaat tgcgattctg
    20461 caatatctgg aaaaaagtgg gggaagttcg tcggttagcg atatttctct caatctggat
    20521 ttgccgctct ccacgacctt tcgcttgctg aaggttttac aagcagcgga ttttgtctat
    20581 caggacagtc aattaggctg gtggcatata ggattaggtg tctttaacgt cggtgcggcg
    20641 tacatccata accgcgatgt cctctccgtc gccgggccgt ttatgcgccg cctgatgtta
    20701 ctttccggcg aaacggtcaa tgtcgcgatc cgtaacggca atgaagcggt attaattggt
    20761 cagttagagt gtaaatcgat ggtcaggatg tgtgcgccac tgggcagtcg tctgccgctg
    20821 catgcttccg gtgcgggcaa agcgctgctt tatccgctgg cggaagagga gttgatgagc
    20881 atcattctgc aaaccggttt gcagcagttt acgccaacta cgcttgtgga tatgcccacc
    20941 ttgctgaagg acctggaaca agcgcgtgaa ctgggctata ccgtagataa agaagagcat
    21001 gttgtaggtc tgaattgcat agcttcagca atttacgatg atgtcggtag agttgttgcc
    21061 gctatctcca tctccgggcc ttcatcaaga ctgacagaag atcgttttgt cagtcagggt
    21121 gagctggtca gagacaccgc ccgcgatatc agcacggcgt tgggactgaa agcacatcca
    21181 tagtgtctgt cgcatcccgc tctgcggagc gggttttttt gacaaaattt gaaagttgga
    21241 aaatttttcc aactaataga ggtaggaata aaatggcaaa aatgagagcc gttgacgcgg
    21301 caatgtatgt gctggagaaa gaaggtatca ccaccgcctt cggcgttccg ggagctgcaa
    21361 ttaatccgtt ctactcagcg atgcgtaagc acggcggtat tcgtcacatt ctggcgcgtc
    21421 atgtggaagg tgtttcgcac atggcggaag gttatacccg cgcaacggca gggaatatcg
    21481 gcgtatgtct ggggacttcc ggtcctgcgg gcacggacat gatcaccgcg ctctattccg
    21541 cttctgctga ttccattcct attctgtgca ttaccggcca ggcaccgcgc gcccgtctgc
    21601 ataaagaaga ttttcaggcc gtagatattg aagcaattgc taaaccggtc agcaaaatgg
    21661 cggttacagt tcgtgaagcg gcgctggtgc ctcgcgtgct gcaacaggca tttcacctga
    21721 tgcgttctgg tcgtccgggt ccggtactgg tggatttacc gttcgacgtt caggttgcgg
    21781 aaatcgagtt tgatcctgac atgtacgaac cgctgccggt ctacaaacct gctgccagcc
    21841 gtatgcagat cgaaaaagct gtagaaatgt taatccaggc cgaacgtccg gtgattgttg
    21901 ccgggggcgg ggtaatcaat gctgacgcag ctgcactgtt acaacagttt gctgaactga
    21961 ccagcgttcc ggtgatccca acgctgatgg gctggggctg tatcccggac gatcatgaac
    22021 tgatggccgg gatggtcggt ctgcaaaccg cgcatcgtta cggtaacgca acgttgctgg
    22081 cgtccgacat ggtgtttggt atcggtaacc gttttgctaa ccgtcatacc ggttcggtag
    22141 agaaatacac cgaagggcgc aaaatcgttc atattgatat tgagccgacg caaattggtc
    22201 gcgtgctgtg tccggatctc ggcattgtct ctgatgctaa agcggcgctg acactgctgg
    22261 ttgaagtggc gcaggagatg caaaaagcgg gtcgtctgcc gtgtcgtaaa gaatgggtcg
    22321 ccgactgcca gcagcgtaaa cgcactttgc tgcgcaaaac ccacttcgac aacgtgccgg
    22381 tgaaaccgca gcgcgtgtat gaagagatga acaaagcctt tggtcgcgat gtttgttatg
    22441 tcaccactat tggtctgtca caaatcgctg cggcacaaat gctgcatgtc tttaaagacc
    22501 gccactggat caactgtggt caggctggtc cgttaggctg gacgattccg gcggcgctgg
    22561 ggggttgtgc cgctgatccg aaacgcaatg tggtggcgat ttctggcgac tttgacttcc
    22621 agttcctgat tgaagagtta gccgttggcg cgcagttcaa cattccgtac atccatgtgc
    22681 tggtgaataa cgcctatctc ggcctgattc gtcagtcaca acgcgctttt gacatggact
    22741 actgcgtgca actcgctttc gagaatatca actccagtga agtgaatggc tacggtgttg
    22801 accacgtaaa agtagcggaa ggtttaggtt gtaaagctat tcgggtcttc aaaccggaag
    22861 atattgcgcc agcctttgaa caggcgaaag ccttaatggc gcaatatcgg gtaccggtag
    22921 tcgtggaagt tattctcgag cgtgtgacca atatttcgat gggcagcgaa ctggataacg
    22981 tcatggaatt tgaagatatc gccgataacg cagcggatgc accgactgaa acctgcttca
    23041 tgcactatga ataagggaga taaatgatgt tacgtttctc tgctaattta tcgatgttat
    23101 ttggtgaata tgattttctc gcccgttttg agaaagctgc gcagtgtggt tttcgcggcg
    23161 ttgaatttat gtttccttat gactacgaca ttgaagaatt aaaacaggta ctggcgagta
    23221 ataaactcga acatacgctg cacaatttac cggcaggtga ctgggcggcg ggcgagcgcg
    23281 gtattgcctg tattcctggc cgtgaagaag agtttcggga tggcgtagca gcagcgatt
//
LOCUS       DAJTSF010000063        22412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000063 DAJTSF010000000
VERSION     DAJTSF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.063"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            82..2682
                     /locus_tag="OO335_002561"
     CDS             82..2682
                     /locus_tag="OO335_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR6002903.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3656
                     /locus_tag="OO335_002562"
     CDS             2673..3656
                     /locus_tag="OO335_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6002904.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3754..4294
                     /locus_tag="OO335_002563"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="OO335_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="OO335_002564"
     CDS             4302..4817
                     /locus_tag="OO335_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6002905.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="OO335_002565"
     CDS             4783..5520
                     /locus_tag="OO335_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6002906.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="OO335_002566"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="OO335_002566"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6002907.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="OO335_002567"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="OO335_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6002908.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="OO335_002568"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="OO335_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6002909.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="OO335_002569"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="OO335_002569"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6002910.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="OO335_002570"
     CDS             10827..12734
                     /locus_tag="OO335_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6002911.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="OO335_002571"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="OO335_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6002912.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="OO335_002572"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="OO335_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6002913.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="OO335_002573"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="OO335_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6002914.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="OO335_002574"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="OO335_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6002915.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="OO335_002575"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="OO335_002575"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6002916.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="OO335_002576"
     CDS             complement(17402..19162)
                     /locus_tag="OO335_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6002917.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="OO335_002577"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="OO335_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6002918.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="OO335_002578"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="OO335_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6002919.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="OO335_002579"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="OO335_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6002920.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22005..>22412
                     /gene="sxy"
                     /locus_tag="OO335_002580"
     CDS             22005..>22412
                     /gene="sxy"
                     /locus_tag="OO335_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR6002921.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT         5721 a         5258 c         5873 g         5560 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 tgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ccaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca
    21841 acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa
    21901 acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt
    21961 taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct
    22021 ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat
    22081 cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg
    22141 gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg
    22201 tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg
    22261 aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag
    22321 cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt
    22381 cttttcatct ggaagcgatt ctcggggagg tg
//
LOCUS       DAJTSF010000064        22281 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000064 DAJTSF010000000
VERSION     DAJTSF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.064"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1028)
                     /locus_tag="OO335_002581"
     CDS             complement(<1..1028)
                     /locus_tag="OO335_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6002922.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1130..2110)
                     /locus_tag="OO335_002582"
     CDS             complement(1130..2110)
                     /locus_tag="OO335_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR6002923.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2118..2828)
                     /locus_tag="OO335_002583"
     CDS             complement(2118..2828)
                     /locus_tag="OO335_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR6002924.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3045..4868)
                     /gene="typA"
                     /locus_tag="OO335_002584"
     CDS             complement(3045..4868)
                     /gene="typA"
                     /locus_tag="OO335_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6002925.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5241..6650
                     /gene="glnA"
                     /locus_tag="OO335_002585"
     CDS             5241..6650
                     /gene="glnA"
                     /locus_tag="OO335_002585"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6002926.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6823..7872
                     /gene="glnL"
                     /locus_tag="OO335_002586"
     CDS             6823..7872
                     /gene="glnL"
                     /locus_tag="OO335_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6002927.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7884..9293
                     /gene="glnG"
                     /locus_tag="OO335_002587"
     CDS             7884..9293
                     /gene="glnG"
                     /locus_tag="OO335_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6002928.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9405..9515
                     /locus_tag="OO335_002588"
     CDS             9405..9515
                     /locus_tag="OO335_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6002929.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="OO335_002589"
     CDS             complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="OO335_002589"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6002930.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="OO335_002590"
     CDS             complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="OO335_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6002931.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12397..13029
                     /locus_tag="OO335_002591"
     CDS             12397..13029
                     /locus_tag="OO335_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6002932.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13411..16197)
                     /gene="polA"
                     /locus_tag="OO335_002592"
     CDS             complement(13411..16197)
                     /gene="polA"
                     /locus_tag="OO335_002592"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6002933.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16561..17493
                     /locus_tag="OO335_002593"
     CDS             16561..17493
                     /locus_tag="OO335_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6002934.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17534..18964)
                     /locus_tag="OO335_002594"
     CDS             complement(17534..18964)
                     /locus_tag="OO335_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6002935.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="OO335_002595"
     CDS             complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="OO335_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6002936.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="OO335_002596"
     CDS             complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="OO335_002596"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6002937.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20825..21094)
                     /locus_tag="OO335_002597"
     CDS             complement(20825..21094)
                     /locus_tag="OO335_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6002938.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21164..21748
                     /gene="mobA"
                     /locus_tag="OO335_002598"
     CDS             21164..21748
                     /gene="mobA"
                     /locus_tag="OO335_002598"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6002939.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21730..22257
                     /gene="mobB"
                     /locus_tag="OO335_002599"
     CDS             21730..22257
                     /gene="mobB"
                     /locus_tag="OO335_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6002940.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5515 a         5583 c         5333 g         5850 t
ORIGIN      
        1 tttgcttcgc cgataggtgc aaaaaatacg gctcgctgtg taaagactat cgcgccaaag
       61 cccagcgtac atgccatccc taaatagacc ggcatacttt cgtgcggcag cataatcaac
      121 agtacgagcg cagcggtact gatgataaag gtgtagcata gatatttact cggcgatttc
      181 aggatcttat ctgaaatcat gccaccaatc ggtccaccaa tcattttcag acagtattgg
      241 ttaatgatgc cgtaagcccc caccagcgca acgggcaata gatagatgtt tttcaggaat
      301 ggaatgaaga atgtcaggcc acagtaaacc gcgtaaacga agaagacgtt gaaagcgata
      361 agccagatgg ttttgtctct cagcactgag gtcatactgg tgttttgcga tgctccgtct
      421 tcttttttca cctcaacgga cggtgcctct tctttgtcat tcaggacaaa gaaaataata
      481 atgcctacgg caatcacaat aagggaatag aaccagatgc ctgctttaaa acctaataag
      541 ccactgccaa accaggtaaa tactgccaac gcagaaaatg ccaccacggt atcgacaatg
      601 ccacgccctg tttcgaagaa gccaaacaac cgaccttgtt gttcgctatt gcccaatcga
      661 cttaccgact tgagcaagac cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc
      721 cagacaaaga ggatgcccca ataccccggc attgtcgtca aataaacacc cagtaatcct
      781 gtcgcaatga gtgagaatga catggtgtat ctgcgcggta gtttatcggc aaaataaata
      841 gacagaaaaa agcccactgt ggtgacaaat gagtttaccg acatagcatt accaatttga
      901 ccattggtca aatggaaata ttcctgcatc gggatataaa acgcatcttt cagcgacggt
      961 aatttataaa ttgtaccgcc acacagtgtt aatagactaa ataacgccca tttctttttc
     1021 gtgagcataa catcccctaa gcgattttta aaatcactgt tttaaaggca ataagatacc
     1081 gaccattcat atggaatggt tttattaact gcgtatgagt taaaacttat tagtggttca
     1141 gaagatggtt tgcctcttgt ttgatttgtt gtagtacgtt acgcacatga tttatctgat
     1201 taattgcatc ttctttttct ttacgtaacc gcgcatccag taaatgattt tctggtaggg
     1261 gtgtttcact catctggcga taagggatcc acggattatc atcttcgtct tcaaagcgga
     1321 acgccgacgc atagtaatca acctcttctt ccaggccata tgccgtcacc tgcggctcat
     1381 catcgccaag gcagataagg tgcgttaata acgctttgaa tggcatatca ccctgacctg
     1441 aaatacaggc tttatgaccc aggccaccct gttctttaac gatcaaggca tctttaatat
     1501 ggacctgggt aatatgcggt gccatcgttt ttaaggcgtc gatgggatgc tcatttgcat
     1561 tgatcatgtt agcaaaatca aacagtaagg aaagagactc catctcactt tctttgacca
     1621 gcgacaccag ctcatgactt tttaaatctt catgctgctc aatcgtaaaa gtcaggccgc
     1681 tgtcctgata cgtttcccgt acataggcaa tgtcgttagc gataatcgat aatacgtcgc
     1741 gcaaattacc ttcataacat ggataaaaac gtacggacgt tgccccagtt ttcaacgcga
     1801 tggcgacggc ttcgtcaata gatgccttat ctgaagcgct ggtttcaata tgaatatcaa
     1861 ggttcagacg gcgggcttta tccccaaagg cagagagttc tttatcgtcc atattaccaa
     1921 gagaaaaacg ctcgccatcc agaacatgga ttttgacgcc acgtagatta ttttcactgg
     1981 cgatatcaag taaatcagca ggcaacactc tttccagccg catatttaaa tgaaaggcat
     2041 aagcatgtaa ataaagagga agagtgtcga cacgttgtag aatctttctt gcgttattta
     2101 ttgtcaccat aaaacaccta cagattaatg atgaggtcta cggcctcaaa tcgccaatat
     2161 tcaatatcta tctggacaat attgccttgc tggtcggcgc gatgcttttc aattaagatc
     2221 gctggcatac ccgcagaacc gccaagatac ttgctgatat cgcccggcat tctgacgggt
     2281 ttgaaaatca atttttttac taccgtctct ttttggtagc acttttccca gactgcagaa
     2341 aatgagtggt tttcaagttg ttcaataaaa cccggagcaa cttcaggatt aatatatgtt
     2401 tcgtgataga aaactttatg tccttccagc gccccccagc cggtgatgcg atagagttca
     2461 gttgatggcg tcactgcaat cagtggcgcg agcgtctcgg gaatatcgct ggtacggttt
     2521 ttctcggtaa acccccaaga aggctctcgc ccttgctcaa ttgcggcccg ctgaaagctc
     2581 gccgacagct ccggactgta attaaatcgc ggctgggtca cgaaccaccc cttacgatcc
     2641 ttacggaaga ttttggattc tgcctcgagg ttcaacaacg cctggcgcag cgtcatacgt
     2701 ttaatgccca gcagttctcc cagctctctt tctgaaggga gttttccacc cggcgtggtg
     2761 atgccatctt tcaaccaccg atccagtttc tcttttgcat tttctacggt ggattgattc
     2821 tccgccattg tttaatcctt ttggtttaaa ccagatgaag catcttacgc ttacgccaaa
     2881 aaagaaagca attaacggtg tatgactttg cagataattg tgacaaagct ctctattgac
     2941 gtaatcacag acttataagg gagatactcg cattggtgtg cgctcacgac aggtaaaaaa
     3001 aaacctgcca tcgctggcag gttttttatg actaaaaaac gaaattaatc gtctttcggt
     3061 gcgcggttgg cgcggcgacg atcgttttcc gtcaggtgac gtttacgaat acggatagag
     3121 gtcggagtca cttctaccag ttcgtcatca tcgatgaact ccagagcttg ttccagagtc
     3181 atgcggatag gcggaaccag aacaacagct tcgtcagtac cggaagcacg catgttggtc
     3241 agtttcttac cggtcaggca gtttacagtc aggtcgttag agcggctatg aataccgata
     3301 atctgacctt cgtaaacttc tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaga
     3361 ccgaacagcg cgaacgcgac agctttaccc tgaccgttag agatcagtac accgttctga
     3421 cgctgaccca cttcgcccgg acgtacgtcg tcgtagtggc tgaaggtgga gtacagcaga
     3481 ccagtaccgg aagtcatggt catgaactca gaacggaagc caatcagacc acggcttggg
     3541 atcacgtagt cgagacgtac gcggccttta ccgtctggat tcatgttttt caggtcgcct
     3601 ttacgttcgc ccagcgcctg catcacagaa ccctgatgct gttcttcaac gtccagcgtc
     3661 acgttttcat acggctcttg tttacgaccg tctatttcac ggaagattac tttcggacgg
     3721 gataccgcca gttcgaaacc ttcacgacgc atgttttcga taagaacaga gaggtgcagt
     3781 tcaccacgac cagaaacgcg gaacgcatcg gcgtcttcgg tttcttctac gcgcagcgca
     3841 acgttgtgta ccagttcttt gttcagacga tcgaggatct gacgagacgt tacgaactta
     3901 ccttctttac cgcagaacgg cgaggtgtta acgcagaaga acatagaaac ggtcggctca
     3961 tcaacggaga gtgccggcag cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg
     4021 ttcagttcgc ccaggcccgt gatcgccacg atatcgccag cttccgccag atcggtttcg
     4081 atacgttcca gaccgaggtg gcccagcact ttaccgactt tcgcgttacg ggttttgcct
     4141 tcgctatcga tgatagtaac ctgctggttc ggcttcactt taccacgctt gatgcggcca
     4201 atgccgataa cgccaacata gctgttgtaa tcgagctgag aaatctgcat ctggaacgga
     4261 ccgtcaaggt caacgtccgg cgcaggaacg tggtcaacaa tcgcctgata cagcggggtc
     4321 atgtcttccg ccatatcttc gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa
     4381 acgatcggga agtccagctg ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc
     4441 tgatccacaa cccaatcagg acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc
     4501 ggcttcaggc cgtaagcaaa cgcttttttg gttacgaagc gcgtttgcgg catcgggccg
     4561 tcaaatgcgt caaccaccag cagcactgag tctaccatgg acattacacg ttcaacttca
     4621 ccaccgaagt cggcgtgccc cggggtatca acgatgttga tacggtaatc attccatttg
     4681 atagcggtgt ttttcgcgag gatggtaatc ccacgctctt tctccaaatc gttggagtcc
     4741 atcacgcgct cttgggtttc ggcacgagag tcgaacgtac cggattgttg gagcagcttg
     4801 tctaccaggg tggttttacc atggtctacg tgcgcgatga tggcgatatt acgcaatttt
     4861 tcgatcacaa ctttgcctca ggcattagaa atagcgcgtt attgtacacg gattaatcgc
     4921 actacaaaac aggatcacaa acatcctccg caaacaagta ttgcagagtc cctttgtgat
     4981 cgctttcacg gagcataaaa agggttatcc aaaggtcatt gcaccaatat ggtgcttaat
     5041 gtttccattg aagcactata ttggtgcaac attcacatca tggtgcagcc cttttgcacg
     5101 atgttaagca tgataacgcc ttttaggggc aatttaaaag ttggcacaga tttcgcttta
     5161 tcttttttac ggcgacacgg ccaaaataat tgcagatttc gttaccacga cgacaatgac
     5221 caatccagga gagttaaagt atgtccgctg aacacgtact gacgatgctg aacgagcacg
     5281 aagtgaagtt tgttgatttg cgcttcaccg ataccaaagg taaagaacaa cacgtcacta
     5341 tccctgctca tcaggtgaat gctgaattct tcgaagaagg caaaatgttt gacggctcct
     5401 cgattggcgg ctggaaaggc attaacgaat ccgacatggt gctgatgcca gacgcatcca
     5461 ctgcagtgat tgacccgttc ttcgccgact ccaccctgat tatccgttgc gacatccttg
     5521 aacctggcac cctgcaaggc tatgaccgtg acccgcgctc tattgcgaag cgcgccgaag
     5581 attacctgcg ttccactggc attgccgaca ccgtactgtt cgggccagaa cctgaattct
     5641 tcctgttcga tgacatccgt ttcggatcat ctatctccgg ttcccacgtt gctatcgacg
     5701 atatcgaagg cgcatggaac tcctccaccc aatacgaagg tggtaacaaa ggtcaccgtc
     5761 cggcagtgaa aggcggttac ttcccggttc cgccggtaga ctctgctcag gatattcgtt
     5821 ctgaaatgtg tctggtgatg gaacagatgg gtctggtggt tgaagcccat caccacgaag
     5881 tagcgactgc tggtcagaac gaagtggcta cccgcttcaa taccatgacc aaaaaagctg
     5941 acgaaattca gatctacaaa tatgtcgtgc ataacgtagc gcaccgcttc ggtaaaaccg
     6001 cgacctttat gccaaaaccg atgttcggtg ataacggctc cggtatgcac tgccacatgt
     6061 ctctgtctaa aaacggcgtt aacctgttcg caggcgacaa atacgcaggt ctgtctgagc
     6121 aggcgctgta ctacattggc ggcgtaatca aacacgctaa agcgattaac gccctggcaa
     6181 acccgaccac caactcttat aagcgtctgg tcccgggcta cgaagcaccg gtaatgctgg
     6241 cttactctgc gcgtaaccgt tctgcgtcta tccgtattcc ggtggtttct tctccgaaag
     6301 cacgtcgtat cgaagtacgt ttcccagatc cggcagctaa cccgtacctg tgctttgctg
     6361 ccctgctgat ggccggtctt gatggtatca agaacaagat ccatccgggc gaagccatgg
     6421 acaaaaacct gtatgacctg ccgccagaag aagcgaaaga gatcccacag gttgcaggct
     6481 ctctggaaga agcactgaac gaactggatc tggaccgcga gttcctgaaa gccggtggcg
     6541 tgttcacgga tgaagcgatc gatgcgtaca tcgctctgcg tcgcgaagaa gatgaccgcg
     6601 tgcgtatgac tccgcatccg gtagagtttg agctgtacta cagcgtctaa tagttgaagt
     6661 tgtactaccc ggcgcacaac gccgggattt agttgccgtg gaaactttca gcccatctct
     6721 gcatgggctt ttttctccgt caattctctg atgcttcgcg ctttttatcc gtaaaaagct
     6781 ataatgcact aaaatggtgc aacctgttca ggagactgct ttatggcaac aggcacgcag
     6841 cccgatgctg ggcagatcct caactcgctg attaacagta ttttgttaat tgatgacaat
     6901 ctggcgatcc attacgccaa ccccgccgcg caacaactgc tcgcccaaag ctcccgcaaa
     6961 ttgtttggta cgccgttacc ggaactgttg agctacttct cattaaatat cgagctgatg
     7021 caagaaagtc tggaggcggg gcaaggcttt accgataacg aagtgacgct ggtcatcgat
     7081 ggtcgctcgc atatcctttc tgtgacggcc cagcgtatgc cggacggcat gatcctgctg
     7141 gagatggcac cgatggataa ccagcgccgc ttaagccagg aacaactaca acatgctcaa
     7201 caagtagctg cccgtgattt agtgcgcggc ctggcacatg agattaaaaa tccgcttggc
     7261 ggtttacgtg gcgcggcgca gttactcagc aaagcgttac ctgacccatc actactcgaa
     7321 tataccaaag tgattatcga acaggcggac cggctgcgaa atttggtcga ccgtctgttg
     7381 gggccgcagc tgcccggtac gcgcgttacc gaaagtattc acaaagtggc tgaacgcgtg
     7441 gtaacgctgg tgtcgatgga actgccggac aacgtgcggt tgattcgtga ttacgatccc
     7501 agcctgccgg aactggcgca cgacccggat caaattgaac aggtcttgct gaatattgtg
     7561 cgcaatgcgc tacaggcgct ggggccggaa ggcggtgaaa tcattctgcg tacccgcacc
     7621 gcgtttcaac tgaccttaca cggcgagcgc taccggctgg cggcgcggat tgatgtggaa
     7681 gataacgggc cgggcattcc gcctcatttg caggatacgc tgttttaccc gatggtcagc
     7741 ggccgcgaag gtggcaccgg gcttggctta tccatcgccc gtaatttgat tgatcagcat
     7801 tcaggcaaaa ttgaatttac cagttggcca ggtcataccg agttctcggt ttacctgcct
     7861 atcaggaaat aaaggtgacg tttatgcaac gagggatagt ctgggtagtc gatgacgata
     7921 gttccatccg ttgggtgctt gaacgtgcgc tcgctggagc gggtttaacc tgtacgacat
     7981 ttgagaacgg cgcggaagta ctggaggcgc tggcgagcaa aacgccggat gtgctgcttt
     8041 cagatatccg tatgccggga atggacgggc tggcgctgct caagcagatt aaacagcgcc
     8101 atccaatgct tccggtcatc attatgaccg cacattccga tctggatgct gccgtcagcg
     8161 cctatcaaca aggggcgttt gattatctgc ccaaaccgtt tgatatcgac gaagcagtgg
     8221 cgctggttga gcgcgctatc agtcattacc aggaacagca gcagccgcgt aatattcagc
     8281 ttaacggccc aacgaccgat atcatcggcg aagcgcaagc catgcaggac gtgttccgta
     8341 ttatcggtcg gctttcgcgt tcttctatta gcgtgctgat taacggcgaa tccggcaccg
     8401 gtaaagaact ggtcgctcat gccctgcatc gccacagtcc gcgcaccaaa gcgccgttta
     8461 tcgcgctgaa tatggcagct atcccaaaag atttgatcga atcagaactg tttggccacg
     8521 agaaaggcgc gtttactggc gcgaatacca ttcgtcaggg gcgttttgaa caggccgatg
     8581 gcggcacgtt atttctcgac gaaattggtg atatgccgct ggatgtgcag acgcgtttgc
     8641 tgcgcgtgct ggcagacggt cagttttatc gcgttggcgg ctatgcgccg gtgaaagtgg
     8701 atgtgcggat tatcgctgcc actcaccaga atcttgaaca gcgggtgcag gaaggtaagt
     8761 tccgtgagga tctgttccac cgcctgaacg ttatccgcgt tcatctgccg ccgctgcgcg
     8821 aacgtcggga agatattccc cgtctggcgc gccatttttt acaggttgcc gcgcgcgaac
     8881 tgggcgtaga agcgaagttg ctgcatccgg aaaccgaagc tgctctgacg cgtctggcgt
     8941 ggccaggcaa cgtgcgccag ctggaaaaca cctgccgctg gctaacggtg atggccgccg
     9001 ggcaggaagt gttgattcag gatttgcccg gcgaactgtt tgaatcaacg gttgcggaga
     9061 gtacttcgca aatgcaaccg gacagttggg cgacactttt agcgcagtgg gcagacagag
     9121 cgctgcgttc cggtcatcaa aatctgcttt ccgaagcgca gccagagctg gagcggacgt
     9181 tactgacgac cgcgttgcga catacgcagg ggcataaaca ggaagcggcg cggctactcg
     9241 gctggggccg caacactctg acgcgtaagt taaaagagct ggggatggag tgattcacgg
     9301 cttgtgtgta aagattgatt attgagcgca aattgctggt attttgcgct ttactgttcc
     9361 gataagttca gtatgatctt gcccggaaaa cggggagagt cattatgctg gaatcaataa
     9421 ttaatctggt atcgagtggc gcagttgaca gccacacacc gcaaactgcc gttgctgccg
     9481 tgctgtgtgc cgcgatgatt gggctgttta gctgaagctc gttgtttatg ccggatgcgg
     9541 agaaagtcgt aggcctgata agacgcgacc agcgtcgcat caggcatcat gctcagatgc
     9601 ctgatgcggc gtaaacgcct tatccggcct acaaaagcaa taatttcaac aggttttgtt
     9661 gcccagcgca tcaggcaatc ttgttctcag cacattgatg gcgctttcgt tttctacttt
     9721 gtaaacgaag cgccattcac tctttaaatc acccgtgaga actgctgcat ccgcgctttc
     9781 tggcgcagat aggtatcaaa gcacatgcaa atgttgcgga tcagcaagcg acctttcgcc
     9841 gtcacctgta ttcccttctc atccacatcc accagcccat cttttgctaa cggggcgagc
     9901 agcttgagat cttccgcaaa gtaatcagcg aagtgcaaat cccactgttt ctcaagaggg
     9961 gcgtaatcca gacggaagtt gcagatgagc gacttaatca catcgcggcg aatacagtca
    10021 tcacgcgtta gcgcaatacc acgccacagc gcattgcctt gttcatacac ttgctgatag
    10081 tactgcttca actctttctg gttctgcgcg tagcagtcgc caatcatgct gatggcggaa
    10141 acgcccatcc ccagcagatc ggtatcgccc tgagtggtgt agccctggaa gttacgatgc
    10201 agcacgcctt cacgctgggc caccgccagc tcgtcatcag gacgggcaaa gtgatccata
    10261 ccgataaact gatagcccga ttgcgtcagg aaggcgatgg tttcctgcag gatatcgagt
    10321 ttttgctgcg gactcggcag gtcagcatct ttgattttgc gctgagcagc aaaaatggtc
    10381 ggcagatgcg cgtagttaaa aacgctcaga cgatcggggt tcagctccgc cacacgtttc
    10441 agggtaaagg cgaaactctc cggcgtctgt ttcggcaggc cgtaaatcag gtcgatgttg
    10501 gtggaggtaa aaccaatctc acgcgcatgg ttaagcagtg caaagatgaa ctcttcatcc
    10561 tgctcgcggt taaccagacg ttgcacttct ttgttgaagt cctgcacgcc catgctcagg
    10621 cgattaaagc cttcggcgcg taaatgatcg agtacatcca gttcgatttc ccgcggatcg
    10681 acttcgatcg aaatctccgc atcggtattg aaatggaagt tttcgcgcag cagcttcatc
    10741 agacggctga tttgcgcttt attcagatac gtcggcgttc cgccgcccca gtgcaattgg
    10801 ctgacgtgac gcccggcgaa cagcggtgca cgatggacga tttcttgctc cagcgcgtcc
    10861 agatactgat cggccttgtg ctgctggcga gtaacaatct tattgcaacc gcagaagtaa
    10921 caaagcttat ggcagaacgg gatatgtacg tagagagata atggacgctc aggatagcgc
    10981 gccacggctt gtaaaaacgc ctgttcgccg aagtcttctg aaaactccag cgcggtcggg
    11041 tacgaggtgt atcgtggccc ggaatagtta tatttctgga tcagggccag atcccagtcg
    11101 atttgctgta cagacatgct cactccttcc gatggcgtct ctggcggcta cggcgtaccg
    11161 gcggatgccc gtttcgggtc atcagccggt tgcgcagcca cttttgtcgc cgcgacaacc
    11221 gctgtagttt aacgaataac cacaccagat aacatataac cggaagggtt atcagcagaa
    11281 cgggaaggcc cacggcggca atccgttagt tgccccgcag cagacgcatc atgtcttctt
    11341 gcttctcgtc ttcttcctct tcttcgtcat cgtcataaga gaggccgagt ttctgcatca
    11401 actcatcaat acgatccagt ttagcatcca cccaggattg ctcttcggcg ctcagggttt
    11461 cgcctgcttc cagacgttcc agcagcgcat ccagacactc atccgtttcc agtaattcca
    11521 actccgcctg cggtgaaagc ataggtttct cactcttcgg tttgtgctgt ttggtgactt
    11581 tttcagtcac tcccaatgga ataggggttt tactgccaat acgtggatct tttggtgcgt
    11641 tctggccttt gctgcctgac gtggtgtttc cgcccgctgc gcggctgccc ggcgcatgac
    11701 cacgacgttt tttctgacgc ttacggtcac gagcttcctg atccagctcc tcgcgtgttt
    11761 ttcgacgcgc ttttgcatga cctttgctgc gtgagtttga agatgatggt ttcataatgg
    11821 ttcgtctttt agccgtttta ttcagtatag atttgcggcg gaatctaaca gaaagcaagc
    11881 aaagaaaaaa ggcgacagat tactctgtcg ccttttttcc tgactcataa cccttaacgg
    11941 gtcttacaat ccttgcaggc cgccaacaca ccctgttttt ccattagccg gaacaatccg
    12001 tgttgattcc atttccgttt aattacgtct ccggacgttt gtcttcctga caatcctctg
    12061 tcttcgcctc ctggcgctcc tgaccctcat cctgagtcat tgttcctgtt agcgtcctcg
    12121 ctctatgggt gctactttac gcctttgctt taaacaaaca atcaaccgcc atcagtattt
    12181 gcacattttc agagttttat ataaaagcaa ttcactgatt tatatagctt atttgacatg
    12241 catcgcagaa ttttctctgc tatttcgcat aaagactatc ggcaatgtct cacaaagcgt
    12301 atggctttta cttacaacgc tcacgccgaa aaatccgcct tttattatgg ttcaggtata
    12361 atcgccacaa agctttgact gacggagacg accgctttga ctaatttgaa ttatcaacag
    12421 acgcattttg tgatgagtgc gcctgatatt cgccacctac cttccgatac cggaattgaa
    12481 gtggcttttg caggccgttc caacgcaggt aaatccagcg cgctgaacac gctgactaac
    12541 cagaaaagcc tggctcgtac ctcaaaaacc ccagggcgca cccagcttat caacctgttt
    12601 gaagtggctg acggcaagcg tctggttgac ttgcctgggt acggttatgc ggaagtcccg
    12661 gaagagatga agcgcaaatg gcagcgtgcg ctcggcgaat acctcgaaaa acgtcagagc
    12721 ctgcaaggtc tggtggtgct aatggatatt cgccatccgc tgaaagattt ggatcagcag
    12781 atgattgagt gggcggtaga cagcaatatc gccgttctgg tgctgctgac caaagcggac
    12841 aaactggcaa gcggcgcacg taaagcgcta ttgaatatgg tgcgtgaagc tgtactggcg
    12901 tttaacggtg atgtgcaggt tgaaacgttt tcttcgttga agaaacaagg cgtagacaag
    12961 ctgaggcaga aactggatac ctggtttagc gagatgcagc ctgtagaaga aacgcaggac
    13021 ggcgaataat tttcttgcct taatgcttgt gccggatgtg gcgtatccgg cccgtaaatt
    13081 caatagatta caattctacc gttggcctga taaacatagc gcatcaggca ttacggatct
    13141 tttctttcgc ccaataaaaa acgccccagt cattactgac tggggcggct aaaatattca
    13201 gccaaatccg attacgtgaa gtaaaaggtc tgaaagatag aacatcttac ctctgtaccc
    13261 tacgcgacta actttactct tttttgttca gttcagaaag cactttttgt aatttttttt
    13321 cagttgttgc ataggaattt ctaatagcca tcacaaaacg tgacagctta tgttgcttac
    13381 ttacgaaaaa agggcatgtt caggcgaatc ttagtgcgcc tgatcccagt tttcgccact
    13441 ccccacttcc accagcaacg gcacatccag acgggtacag ttttccatca gttgatgaat
    13501 ctgcttcgcg acggcatcaa catcatcttt atgaacttca aataccagtt catcgtgtac
    13561 ctgcatgatc atacgtacac gcggttgctc agcctgtaac cacgcatcaa cggcaatcat
    13621 cgcccgtttg ataatgtcgg cggcggttcc ctgcattggc gcgttaatgg ctgcacgttc
    13681 agccgctgca cgacgagcac cattgctgga tttgatatcc ggcagataca gacggcgtcc
    13741 gtccagcgtt tcaacgtagc ccagctcttt cgcctgagca cgggtgcgtt ccatatactc
    13801 cagcacgcca gggtagcgtt cgaagtaaag gtccatgtac ttctgcgctt ctttacgtgg
    13861 aatgttcaat tgccgcgcca gaccgaaagc actcatgcca taaatcagac caaagttgat
    13921 cgctttcgcg ctacggcgtt gctcgctggt gacggtttcc agtggcaaac caaacacttc
    13981 tgccgccgtt gcccggtgga tatcttttcc ttccgcgaat gcggtcagca agcctttatc
    14041 acgcgagaga tgcgccataa tgcgcagttc aatctgcgag tagtccgctg agacaatcac
    14101 ataatcctct ggcgcaataa acgcctggcg gatacgacga ccttcttcgt tacgcaccgg
    14161 aatgttttgc aggttaggat cggttgacga taaacgtccc gttgcagtta ctgcctggtg
    14221 ataagaggta tgcacacgcc cggttttcgg gttgatcatc agcggcagct tgtcggtgta
    14281 agtcgacttc agcttcgcca gaccacgata ctccagaatc acttttggca acggatagtc
    14341 cagcgccagt tcttccagta cctcttccga cgttgacggc gcgccacccg gcgttttctt
    14401 cagcggttta ataccctgtt tttcaaacag aatggtttgc aactgcttgg tggaagaaag
    14461 gttaaattct tcacctgcaa tttcatgcgc tttcttttcc agctcagcca gacgcaaggt
    14521 gagctcttca gaatgattgt gcagcacttt cggatcgatc ttcacaccgt tacgttcaat
    14581 gcgtgaaagc accggcacca gcggcatttc gatattctcg aagacgttca acggcccttt
    14641 gtgtttctgc agatccggcc acattttcag atgcaactgc aaggtgacat ctgcatcttc
    14701 ggcggcgtaa cgtccggcct cttcgagggc aatctggtta aaggtcagtt gatttttgcc
    14761 tttacccgca atctcttcaa aagtgatggt tttgtgcttc aaccaacgtt ccgcgaggct
    14821 gtccatatcg tgacgcccgg caacgctatt gagaatgtag gactccagca tggtatcaaa
    14881 cgcaatccca cgcagttcaa tgccgtaatt cgccagaata ccgcgatcgt atttcaggtt
    14941 ttgcccgacc ttcagcgcct tttcatcttc cagcagcggt tttagcaact cgagtgcacg
    15001 ctcgtgagag atttgatcgg gcgcatcaag ataatcatga gcaaccggaa tatatgccgc
    15061 tacgcctggc tcgatagcaa aagaaagccc gaccaggtta gcagagatgt tatcaaggct
    15121 gtcggtttcg gtatcaaatg caaataccgg cgctttttcc agcttcgcaa tccacgcttt
    15181 cagtgtttct tcatcaagga tagtgacgta gttgtcataa gaaatcaccg ttgccgtcac
    15241 ttctggcgct tcgtctgcaa cactggtttc ctgaggcttc gcggctgttt ttgccccttt
    15301 ggcctgtaac catttgccca cttcgacatc agcagtccag cgtttgaact catacttttt
    15361 gaacagcccc aacaactctt ccgctgccgg ttgctgcact tccagttgtt cacaggtcag
    15421 ctccagttca acatcggttt taatcgtcgc caactggtat gagagataag caacttcttt
    15481 gttttgctcg agcttcgctg ccattgtttt cgcgccacgg aagctcaacc cagcaatttt
    15541 ttctggctcg gcatacagcg tatccagccc gccaagacct tgcagcaatg cctgcgcggt
    15601 tttttcaccg acgcccggta cgccaggaat gttatcagag gagtcaccca tcagcgccag
    15661 gaagtcgatg atcaattctg gcggcacgcc gtacttattc accacctctt ccggtccgag
    15721 gatggtattc gtcatggtat tgataagcgt aatatttggc gtcaccagct gcgccatatc
    15781 tttatcgcca gtgctaatca gcaccggacg cccggctttt tcggcttcgc gcgccagagt
    15841 accgataacg tcgtccgctt ctacgccaga aaccgccagc agcggcagtc ccatcgcttt
    15901 aaccatcgcg tgcagaggtt cgatttgtgc tcgcagatcg tccggcattg gcgggcgatg
    15961 tgatttgtaa tgttcaaaca gttcatcacg aaaggttttt cccttggcgt caaagaccac
    16021 cgctgcatgc gtcggtttat attgcatgat cagactgcgc agcatgttga ggacaccata
    16081 catcgcaccg gtcggctcgc ctgcgctgtt agtcagcggg ggaaacgcgt gatatgcgcg
    16141 ataaagataa gatgaaccat ctacaaggat aagtggattt tgggggatct gaaccataat
    16201 gtccgtgcct gtttatcaga ttatgggtaa aggatgccac agacaggatg aaaacatgag
    16261 tttttcaccg cttttcgtga aaatcttttg tagatctgct ggatcgctcg caaatcgtca
    16321 tgtggataac tttgtgcata aattatttac atcgctttta atttattatc atgcttttta
    16381 cagcatgatg tttgtctttg tttttcatgc agttaaaaat aaatccatcc ctaataattg
    16441 agataatggg attttttcta atgtggatag atatgaatta tttttctcct taaggatcat
    16501 ccgttatttg ggtcattttc tgctaaaatc tgcgccttgc gcttctttag tgcactgtct
    16561 atggctaacc ttttgaataa attcattatg acgagaatac tcgctgcgat aacccttttg
    16621 ctgagtatcg tattgaccat tttggtcact attttttgtt ccgtcccgat catcattgcc
    16681 gggattgtaa aactattgct acctgtgcca gtcatttggc gaaaggtttc gcgtttttgt
    16741 gattttatga tgtattgctg gtgtgaaggt ctggcggtat tactgcatct gaacccacac
    16801 ctgcaatggg aagttcacgg gctggaaggg ttaagtaaga agaactggta tctgcttatt
    16861 tgtaaccacc gtagttgggc agatattgtc gtactgtgcg tgctgtttcg taagcacatt
    16921 ccgatgaata aatatttcct caagcaacag ctggcctggg tgccatttct tggcctggcg
    16981 tgctgggcgc tggatatgcc gtttatgaag cgttattccc gcgcttattt attacgtcat
    17041 ccggagcgac gtggtcaaga tgttgaaacc actcggcgtt cttgtgaaaa gtttcgcctg
    17101 catcccacca ccattgtcaa tttcgttgaa ggctcccgtt tcacgcaaga aaaacatcag
    17161 caaacccact ccacttttca aaacttgttg ccaccaaagg cggcaggcat tgcgatggcg
    17221 ctaaatgtac tgggtaaaca attcgataaa ctgcttaatg ttacgctgtg ttatccggac
    17281 aataaccgtc agccgttctt cgatatgtta agcggtaaac tgacgcggat tgtcgtacat
    17341 gtggatttac agcctattgc cgatgagtta catggcgact acatcaatga caaaagcttt
    17401 aagcgccatt ttcagcaatg gctgaactca ttgtggcagg aaaaagaccg gcttctgacg
    17461 tctctcatgt catcacagcg tcagaataag taagttttac tcgcctctga atgagcagag
    17521 gcgagtgagt attttaatga acaaaacgtc cggcacgaga cataaattct tcttcgctca
    17581 tctcctgtcc gttaaaaaca actttacccg gtgtataacg cagctgcagc gaggcggtat
    17641 tgttgtcaat cgtgattaac tgaaacatct gacctaatgt catcatcccg ctgatttgtt
    17701 tatcagcctg cttttgcgct ttttccgcat ccattccttc agataaatta agctgttttg
    17761 cggtttctgt gacgacatta agcggtaact tcacatcaaa attgagcgat ttgatatctt
    17821 tgtttgtgga tgatgaagat ttggctgggt cggcaatact gatatccaga ttggcattta
    17881 gttcgccgag tgcgttcttc catcttaccg gttgtttaat ggtcggctca cttttttgta
    17941 ataacggtaa atactctttg aacaatgcag cattcacttc ttgcagagca acttcatcgt
    18001 ttgctaactc agggtgtgca gcaagctgct tctgcatcgc aatgttatac tggatgataa
    18061 attggcgcac tgctgaggga tcaatagatt cagcaatcaa tgaccactca ccactaccga
    18121 gttgctgatt cccttttgtt aacttatcaa gttcgtaggt cagattgaca ttgatataat
    18181 ctttgtcttt aacgcgatcc agcctggttt ttgcaacgaa agcatcgatt tgggcaacgt
    18241 cttccccgtg attgataatg ttaatctgat taaatttcga ctcgctttca ccaaccggaa
    18301 atttcttttc ttctaatcgc gccagagaat caatggtaaa actttttgcc gttactttgt
    18361 tcattggtga taattgcaga acaatatctt cgactttgcc ttcaatatcc aggttagaca
    18421 atgaactacc atcaccatta aaagtaaact ggccctcacc aaaagaaaat tttccatact
    18481 ctgcaggggc aacatctaat gtcgtagaga atgagccacc aaacgcgaat cgcaatgtcg
    18541 cttccacggg cgatttattt ttcgcggcga taaatagcgg ttgtgtcagt tcattattca
    18601 ctaagttcac ttttgccgct gccagtgctg ggatgacatt accatgcatt aacattgtga
    18661 tgggcaacgg accgtgctca acatccacat caaatacaac tttctggcct ggcttgatat
    18721 tgagatcggg tgcaccgtta tcgaatgtca tttgcatttg aaaacgggaa ttaaaaaagc
    18781 ctttgtcaaa gtttttataa cttaacgtca tatcgtaggc atgttcacct ttctttttcg
    18841 catcgttaaa atctttaata aacttttcga taccaggctg aatttgcgta cctgtgtacc
    18901 atgtgccacc accccagatt acggctaacg caacaataac acctgtagct gacttacgta
    18961 tcatataaat attccctgat aaaacagcaa aactttgaat atccacttat gctgaaataa
    19021 tgtccagcgg caggatgcat tatcagaatg tatttatatt tattaaataa taaaaaaagc
    19081 ccgtgaatat tcacgggctt tatgtaattt acattgaatt atttttcctc ggacagatat
    19141 ttcaccgtat cagcatactg ctgaacaaaa acatccatat tgctggtatc cataccctgc
    19201 ggattcagct gatatttacc gttaacaaac atcgccggaa caccacgcaa ttgcacatca
    19261 gctgcggctt tttcctgctg agcgaccaga gatttcacca cgaagctgtt ccacgccgcg
    19321 tcgtactctt cacctttaat acctgcattg ataaatacat cgcggatatc agatgctgaa
    19381 cgaatggtct gggttttctg tacgccttca aacagcggaa cagtcacttt gtcttccacg
    19441 cccagcgcca tcgccacagc ccatgcctga gtcagatctt tgcccaggtc accacccatg
    19501 aagttgacgt ggtatttagt catcttcacg ccttccggca gttttttctt cacattatca
    19561 gaaatatgca gaacttcttc aaactgatag cagtgcgggc agaagaaaga gaaaaactcc
    19621 agcacttgcg gcgcgccagc taccggtttt tccagggtag tgtactgttt accatcttca
    19681 tactgcgccg ccgatgcgct aaacgctaaa actaaaccag ccagcgccag ccaaatcttt
    19741 ttcatgatct actctctccg attaatacat aggtgttaat tgcaaagggg gttcttgtag
    19801 aacttttgcc tgttctataa aagtcgccgt ctgtcgtagc cagtaatctt ccccggttaa
    19861 ccacgggaaa tttttcggga acgcgggatc agcccaatgc cgcattagcc aggcaagata
    19921 ataaaccaaa cgcatggcgc gtaaaggttc aatcagtccg atttcagcgg tgtcgaactc
    19981 gctaaattct tcataagctt caataatagt ttccagttgc atccgctgct gggctttatc
    20041 gccattgagc aacatccaca aatcctgaat agctggacca ttacgtgcat catccagatc
    20101 aacaaacatt ggaccatcgc gccagagaat attcccggcg tggcagtctc catgtagccg
    20161 cagaacggtg aaatcttccc gccagtgtgc tgtaacggcg gcgatcagct catctgtcgc
    20221 tttcagaaat gccgctttca acccggaagg tatcagtgta gcgtcctcaa acagcttgcg
    20281 aggctcaatg agatattcat tcagaccgat ggtcgggcga tgaataaaaa gctgtttgcg
    20341 ccccgtctgg tgcatacgcc ctaaataacg cccaaccgct tccatctgat cgatattatc
    20401 agcttcgaac tggcgaccac cgacgcttgg aaaaacagcg aaataaaatc cctgatgatt
    20461 caataaagtc tgaccgttaa aggccacagg tgctgcgacc ggaacttcat catttaccag
    20521 ctgcaacgca aattgatgtt cttcgaggat ttgatcggct gtccaacgtt cagggcgata
    20581 aaatttgacg acaaaacgtc gacgctcttc gtcctgaaat tgatagacac ggttttcata
    20641 gctgttaagc ggggtaagac cggaatccac ccggatccct tgcttaaaca gagcgtccat
    20701 gatggtatcc gggtgtagtg tctggaaagt aaaagcgctg ttgttcatcc catcatccgg
    20761 aaaacattac gaatgatgaa ggatatcatt tcacgatgat tttggtggcg ccgcttacaa
    20821 gcttttactc tttaattacg ccgcgagcgc gtaacagcgc cgttttgaag tcttcttcat
    20881 aatctttctg caaacccggg atcaccgcat ctttcgcgga atcacgcatt ttcaggtgat
    20941 agatcagaat gtcatccgtc aaatccgcca gttcgccgtc aaaacctgac tctttcgcca
    21001 gtttctgcaa aaattgcaac aagttaaagt ctggctcttt ttgccaggct ggctggagga
    21061 gttcaataac ttcattcaga cgtttacatt tcatagtaat gctccttact cttgagacag
    21121 acacgttagc agggtcaatc ccacaataaa agaggcgata tcggtgaatc taatgacgac
    21181 gataacaggc gttgtgctgg caggcggtaa agccagacga atgggcggcg tagataaagg
    21241 attgctggac ttaaatggca aaccgctgtg gcaacatgtc gctgacgcgc ttaagacgca
    21301 gctctctcac gtcgtggtta atgctaatcg tcatcaggaa atctatcagg caggcggtct
    21361 gaaagtgatt gaagattcac tggcggatta cccaggccct ctggcaggaa tgctttcagt
    21421 aatgcagcag gaagctggtg agtggttttt gttttgtccg tgcgatacgc cttacattcc
    21481 ccatgattta gccgcccggc ttaatcatca gcgcaaagat gcgcctgtcg tgtgggtcca
    21541 tgacggtgaa cgcgatcacc cgactattgc tctggtaaac cgcgctattg agcctttatt
    21601 actggaatat ctgcaagcag gagaacgccg ggtaatggca tttatgcgtc tggctggcgg
    21661 tcatgcggtt gatttcagcg atcataaaga tgcatttgtt aacgtgaata cgccagagga
    21721 gcttgcccga tggcaggaaa aacgatgata ccgttactcg cctttgccgc gtggagtggt
    21781 accggaaaaa ctacgctttt gaaaaaactg atcccggcat tatgcgccag agggatccgt
    21841 ccggggctga ttaagcatac gcaccatgat atggatgttg ataagccagg caaagatagc
    21901 tatgagctgc gcaaggctgg cgcggtacaa accatcgttg ccagccagca acgatgggcc
    21961 ttgatgacgg aaacaccaga cgaagaagag ctggatctac attttctcgc aagtcgaatg
    22021 gatacctcaa agctggattt gattctggtc gaagggttta agcatgaaga gatcgcaaag
    22081 attgtgctgt ttcgcgatgg agccggacat cgacctgaag aattagtgat agacaggcat
    22141 gttattgctg tagccagtga tgtgccgctt aatcttgatg tcgcattact ggatattaat
    22201 gatgttgagg ggctggctga ttttgtggtg gagtggatgc aaaagcagaa cggataagca
    22261 agagtaggct ggatcagacg c
//
LOCUS       DAJTSF010000065        21506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000065 DAJTSF010000000
VERSION     DAJTSF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.065"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO335_002600"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO335_002600"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6002941.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="OO335_002601"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="OO335_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6002942.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3194)
                     /locus_tag="OO335_002602"
     CDS             complement(2649..3194)
                     /locus_tag="OO335_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6002943.1"
                     /translation="MTKQPEDWLDDVPGDDIEDDEIIWVSKSEIKRDAEELKRLGAEI
                     VDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALDKL
                     KNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKEGN
                     KPPKSARQIFQYLRELAENEG"
     gene            3288..4640
                     /gene="pmbA"
                     /locus_tag="OO335_002603"
     CDS             3288..4640
                     /gene="pmbA"
                     /locus_tag="OO335_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6002944.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4823..5205
                     /gene="cybC"
                     /locus_tag="OO335_002604"
                     /pseudo
     CDS             4823..5205
                     /gene="cybC"
                     /locus_tag="OO335_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5250..5714)
                     /gene="nrdG"
                     /locus_tag="OO335_002605"
     CDS             complement(5250..5714)
                     /gene="nrdG"
                     /locus_tag="OO335_002605"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6002945.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5872..8010)
                     /gene="nrdD"
                     /locus_tag="OO335_002606"
     CDS             complement(5872..8010)
                     /gene="nrdD"
                     /locus_tag="OO335_002606"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6002946.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8404..10059)
                     /gene="treC"
                     /locus_tag="OO335_002607"
     CDS             complement(8404..10059)
                     /gene="treC"
                     /locus_tag="OO335_002607"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6002947.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10109..11530)
                     /gene="treB"
                     /locus_tag="OO335_002608"
     CDS             complement(10109..11530)
                     /gene="treB"
                     /locus_tag="OO335_002608"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR6002948.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11649..12596)
                     /gene="treR"
                     /locus_tag="OO335_002609"
     CDS             complement(11649..12596)
                     /gene="treR"
                     /locus_tag="OO335_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6002949.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12781..12834
                     /gene="mgtL"
                     /locus_tag="OO335_002610"
     CDS             12781..12834
                     /gene="mgtL"
                     /locus_tag="OO335_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6002950.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12975..15671
                     /gene="mgtA"
                     /locus_tag="OO335_002611"
     CDS             12975..15671
                     /gene="mgtA"
                     /locus_tag="OO335_002611"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6002951.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15877..16263)
                     /gene="ridA"
                     /locus_tag="OO335_002612"
     CDS             complement(15877..16263)
                     /gene="ridA"
                     /locus_tag="OO335_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6002952.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16336..16797)
                     /gene="pyrI"
                     /locus_tag="OO335_002613"
     CDS             complement(16336..16797)
                     /gene="pyrI"
                     /locus_tag="OO335_002613"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6002953.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16810..17745)
                     /gene="pyrB"
                     /locus_tag="OO335_002614"
     CDS             complement(16810..17745)
                     /gene="pyrB"
                     /locus_tag="OO335_002614"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6002954.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17749..17883)
                     /gene="pyrL"
                     /locus_tag="OO335_002615"
     CDS             complement(17749..17883)
                     /gene="pyrL"
                     /locus_tag="OO335_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6002955.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18164..18559)
                     /locus_tag="OO335_002616"
     CDS             complement(18164..18559)
                     /locus_tag="OO335_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6002956.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18691..19404)
                     /gene="bdcA"
                     /locus_tag="OO335_002617"
     CDS             complement(18691..19404)
                     /gene="bdcA"
                     /locus_tag="OO335_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6002957.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19475..20068
                     /locus_tag="OO335_002618"
     CDS             19475..20068
                     /locus_tag="OO335_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6002958.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20213..20665
                     /gene="tabA"
                     /locus_tag="OO335_002619"
     CDS             20213..20665
                     /gene="tabA"
                     /locus_tag="OO335_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6002959.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20788..>21506
                     /locus_tag="OO335_002620"
     CDS             20788..>21506
                     /locus_tag="OO335_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6002960.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKAL"
BASE COUNT         5196 a         5322 c         5667 g         5321 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgatgtca tcaccgggaa cgtcgtcgag ccagtcttcg
     3181 ggctgcttag tcatctcagg ctccttaaaa aaagaggcta atgttaccag ttaagatgcg
     3241 cactgaaaaa cggttctctg ttagacttca gagaaactct ctacattatg gcacttgcaa
     3301 tgaaagtaat ctctcaagtt gaagcgcagc gcaagattct ggaagaagca gtttccactg
     3361 cgctggagtt ggcctcaggc aaatcggacg gtgcggaagt tgccgtcagc aagaccaccg
     3421 gcattagcgt aagcacgcgt tatggtgaag tggagaatgt tgaattcaat agtgatggcg
     3481 cgctggggat cactgtttat caccagaacc gtaaaggtag cgcatcgtct accgatttaa
     3541 gcccgcaggc cattgcccgc accgtacagg cggcgctgga tattgcccgt tatacctcgc
     3601 cagatccctg tgccggcgtg gcagacaaag agctgctggc ctttgacgca ccgaatctcg
     3661 acttgttcca ccctgcggaa gtttccccgg atgaagccat tgaactggcg gcccgcgcag
     3721 aacaggcggc attgcaggcg gacaaacgca tcaccaatac cgaaggtggc agctttaaca
     3781 gccactacgg tgtcaaagtt tttggcaaca gccacggcat gttgcagggt tactgctcaa
     3841 cgcgtcattc gctctccagc tgtgtaattg ccgaagaaaa tggcgatatg gagcgtgatt
     3901 acgcctacac cattggccgc gcgatgagcg atctgcaaac gccagagtgg gtcggggccg
     3961 actgtgctcg ccgtacttta tcgcgcctgt caccgcgtaa actctccacc atgaaagcgc
     4021 cggtcatttt tgccaatgaa gtggcaaccg ggctttttgg ccatctggtg ggggcgatag
     4081 cgggtggctc ggtttaccgt aaatctacct ttctgctgga ttcgctgggt aaacaaattc
     4141 tgccggactg gctgaccatt gaagagcatc cgcatctgct gaaagggctg gcgtcgacgc
     4201 cattcgacag cgaaggtgtg cgcaccgagc gtcgcgatat tattaaagat ggcatcctga
     4261 ctcagtggct gctgaccagc tactcggcgc ggaaactggg gctgaaaagc accggacatg
     4321 cgggcggtat tcacaactgg cggattgccg gacaagggct aagcttcgaa caaatgctca
     4381 aagagatggg caccgggctg gtggtgacgg agctgatggg ccagggcgtg agtgccatta
     4441 ccggtgatta ttcccgtggt gcagcgggct tctgggtaga gaatggcgaa attcagtatc
     4501 ccgttagtga aatcaccatc gcaggtaatt taaaagatat gtggcgcaat attgtcaccg
     4561 tcggtaacga tattgaaaca cgcagtaata tacagtgtgg ttctgtgctg ttgccggaga
     4621 tgaaaatcgc cgggcagtaa aaaactggcg cgacctgccg cgctataaac taaaaaatta
     4681 cacaaaatca ttcgcactgc atcgagacgg aaagtgaatg agggccgggg agcgtactcg
     4741 cagtacgtga cccgggcgaa tgagcgtagc cgtcgatgag gcagtgtgaa ggatgaagtg
     4801 taaataaaaa aggaagtgag caatgcgtaa aagcctgtta gctattcttg cagtctcctc
     4861 gttggtattc agttctgcgt cgtttgccgc tgatcttgaa gacaatatgg aaaccctcaa
     4921 cgacaattta aaagtgatcg aaaaagcgga taacgcggcg caagtcaaag acgcgttaac
     4981 gaagatgcgc gccgcagcgc tggatgcgca aaaagcaacg ccgccgaagc tcgaagataa
     5041 atcaccggac agcccggaaa tgaaagattt ccgccacggt ttcgacattc tggtcggtca
     5101 gattgacgac gcgctgaagc tggcaaatga aggtaaaaag aagcgcaggc tgctgcagag
     5161 caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct catttccctg
     5221 ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac ctgattgctg
     5281 ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc gtcgaccagc
     5341 acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag tttatagcct
     5401 gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac cagtttcaga
     5461 atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat cccctggcgt
     5521 ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt ggtaaatggc
     5581 tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc atgaacacac
     5641 ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac aggatagtac
     5701 tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc atccggtaat
     5761 caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc aaatgtaggc
     5821 cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag attaacctat
     5881 ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac cagcgttaaa
     5941 cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg acacacggga
     6001 ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact cgaactcacc
     6061 ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat acggtacatg
     6121 ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa tgtttggata
     6181 ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt caaagtcgat
     6241 cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg tgtagtaacc
     6301 tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc agaagcggtc
     6361 gcacaggttt tcactcggcg tgctgtaaag actgaaacca taacccgttt cttctttcca
     6421 ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc gaagcttctc
     6481 gttgtcgtaa acatgctcgc cgctgaacag cgcgttaatg gtttcgtgga tgccgatgta
     6541 acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat cgtcagcatt
     6601 cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg ctttcacgcc
     6661 ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac gttcatccag
     6721 cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg gcaggttcag
     6781 gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt cattttccca
     6841 cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga cgttcactac
     6901 ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca gcgccagctg
     6961 tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa tcgcaaacac
     7021 cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac ggttacgcag
     7081 gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac caaaggttac
     7141 aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca gcgactggaa
     7201 ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt ccgggatgtt
     7261 ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg gcgccagcac
     7321 ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct gagcaataat
     7381 ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct cggcgttccc
     7441 cattttaaaa ccctgggtca gcatgccttt caggtcgatc aacatgcagt tgaacatcgg
     7501 gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat gtgcctgcac
     7561 cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc cggccagcag
     7621 gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga gtaacgaggc
     7681 gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt tttcacgttc
     7741 aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt atggacccga
     7801 catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt tgcgcccctg
     7861 catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat cgacttccgc
     7921 tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg gcactttgca
     7981 gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag aacaggttat
     8041 ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg tgctttccgc
     8101 aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt agtagcaatt
     8161 atagtcgatt aatacaacat attgggttgg gacgcatttt aaagtctata tatagtgctt
     8221 tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca gagcgtacgg
     8281 atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg aaaaatcaac
     8341 aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg gcttagggaa
     8401 gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg cacagggttg
     8461 tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca tttgccctgg
     8521 ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt gccccttcca
     8581 ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt aattgcccca
     8641 tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt aaaacacaga
     8701 ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc ctaggccaat
     8761 ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca tgggcgtgcg
     8821 gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat cacgaccatc
     8881 gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag taatgcgcgt
     8941 gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg ttccctgcat
     9001 gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac cttcatcacc
     9061 aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat tccatgccac
     9121 gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa agtcaggttt
     9181 agccagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa aattaaaggt
     9241 catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa ggctggtgga
     9301 ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt tcatctcgtg
     9361 caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc catccaggtc
     9421 ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca acccgtcgac
     9481 cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg ctggattctc
     9541 ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt cgctttccgc
     9601 atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg gcgtttctgg
     9661 ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca gcgcctcgcg
     9721 aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga tacgaatccc
     9781 gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt aggtgggatc
     9841 aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga cataaaaggg
     9901 ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt ggataacgcc
     9961 acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat aaatctggta
    10021 gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc tgcaaatgcg
    10081 agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc aggcggtatt
    10141 tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata gccattgcca
    10201 gcgcaaatac ctgccagtag ctcggttgaa tagagagaat tcccggcagg ccccctacgc
    10261 cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga ccagaaccaa
    10321 tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt gcaggctcag
    10381 tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca ttgtgcttgc
    10441 ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac agcgcaatca
    10501 gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata gcaagggtgg
    10561 tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat gccgcaccaa
    10621 tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca tcgccaatca
    10681 tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc agcgaacaga
    10741 cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga gtttcaataa
    10801 cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag cccactttgg
    10861 cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc agataagcgt
    10921 tcatcagctg gggggaaacc agtgtcacgc caagcacgat accaaggatc ggcgtgccgc
    10981 ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa atcgcttcac
    11041 cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc gccagcgtct
    11101 gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc aaaccgccgc
    11161 taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc aactgctcat
    11221 gccatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc tgcgcctgtc
    11281 cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc accacctgaa
    11341 attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt tctttcggtc
    11401 tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg ctcaccgtcg
    11461 caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt tggtttattt
    11521 tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa tatattgcgc
    11581 ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat ttatcgttca
    11641 ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct gcgcccggtt
    11701 acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc aggatctacg
    11761 gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct cgccagttgc
    11821 aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag cgtgtcggtt
    11881 gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc atagccttgc
    11941 ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt gcagaacgcc
    12001 aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac gccgagataa
    12061 ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat tgccccttcg
    12121 tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac cagcgatgac
    12181 tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac tacgccgtcg
    12241 atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga aaactgattt
    12301 tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt ttgaacggcg
    12361 agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt atcgctttgc
    12421 ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat cactgcttca
    12481 acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg ggaaaccgta
    12541 gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt ttgcatcctg
    12601 ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca ttcagcaatg
    12661 ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc cataaatgct
    12721 acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac cggaggttat
    12781 atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg gtaagcccgt
    12841 cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct gctcagcttt
    12901 attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt tcagacagtg
    12961 cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat ttaccttccc
    13021 gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg gcggtcccgc
    13081 cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa ttgtggaaaa
    13141 cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc cgcgaacaac
    13201 atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat ttatgggtct
    13261 gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct tacgccacgg
    13321 aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg ttgctgaact
    13381 ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg gtcagtaata
    13441 ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg gagatcccga
    13501 tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg atcccggcag
    13561 atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta accggtgagt
    13621 ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat ccgctggagt
    13681 gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa gcaatggtga
    13741 ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt gagcaggaaa
    13801 gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg attcgcttta
    13861 tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc gactggtggg
    13921 aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg ttgccgatga
    13981 ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa gtgatcgtca
    14041 aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact gataaaaccg
    14101 gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc ggtaaaacca
    14161 gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga cttaaaaatc
    14221 tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg ctggccagcc
    14281 gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg tcggtggtag
    14341 tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag gaaatcctca
    14401 atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac accatgctgc
    14461 gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg gttgcggtgg
    14521 cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa tccgacctga
    14581 tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct ccggcactga
    14641 aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt gagttagttg
    14701 cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt ggtagtgata
    14761 ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg ctgtttgccc
    14821 gcctgacgcc gatgcataaa gaacgcattg tgactttact gaagcgcgaa gggcatgtgg
    14881 ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg gatatcggca
    14941 tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc ctgctggaaa
    15001 aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc gccaacatgc
    15061 tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc gtgctggtgg
    15121 cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt cagaacctgc
    15181 tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag caaattcaaa
    15241 agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc ggaccgatca
    15301 gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat gccaacacgc
    15361 cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg tcgcaaacgc
    15421 tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt gcatcgtggc
    15481 cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg ttttcaccgc
    15541 tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg ctggttgcga
    15601 ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc cgtcgttacg
    15661 gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag acaaagtgag
    15721 ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc aataaattgc
    15781 aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt gtcatcagac
    15841 ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag cgattgcttc
    15901 gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acgcaagaac gtgccgggaa
    15961 agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg cgaagtcgtt
    16021 cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc cagcggcttc
    16081 gacgattgct tttacattat ccagcgactg acgtgcctgt gcagcgacgt ctgccggtac
    16141 ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat tgcccagatc
    16201 aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga tagttttgct
    16261 catgatttct cctttattac agcggtaaaa agtctggctc attataggga gccagacttt
    16321 tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt tttcacagta
    16381 tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg atgaaaccgg
    16441 ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga tgcgctctgg
    16501 cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa ccgtggcttg
    16561 cggtgcatac aatgccagtt ggtctacttg ctcttcactc aaaaaggtat tttcgatttt
    16621 gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg tgatgcgctg
    16681 atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg cggggatatg
    16741 gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt gtgtcatctc
    16801 tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg cctggcgagc
    16861 gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat ccacatccgt
    16921 cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt tggcgttgtg
    16981 gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg acgggtccag
    17041 acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca cttcttcaat
    17101 agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa tgtattgcgg
    17161 cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact tcgctagcgc
    17221 ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg cgacgtggag
    17281 attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg tttgcgtcgg
    17341 atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa attcggtggc
    17401 taggcgtgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt aagtgctgat
    17461 aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat tggcgctgtc
    17521 ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag agaggcgggt
    17581 acgggtagag gcttcgaaga aacagctggc aatgactttg tgcttcaaca gctctggttg
    17641 cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat cgcgactaag
    17701 gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt atctcctgac
    17761 acctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca acgggaagaa
    17821 aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa cacactgaac
    17881 catacatcct cccggcaaat tgtccggcat tatactcata gccagaagcg gtgcaagcat
    17941 ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa aatatatgca
    18001 tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat tgcagacatt
    18061 tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc cattcatccg
    18121 acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc tcagggatac
    18181 gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc accgccgtcc
    18241 agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg tcttcagatt
    18301 gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa gtgcatcccg
    18361 cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc tgaaaatcgg
    18421 gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc aaatcgccgg
    18481 aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca gcagggaaaa
    18541 cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca gaagatttat
    18601 atttatcggg agccgctttt agggggacga cgttagtaaa caaaacccag acatcatgga
    18661 taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat caatggtatg
    18721 catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca taccagcgac
    18781 ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat cgcgcattgg
    18841 cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt tgatggtgat
    18901 ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg cagatttact
    18961 ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg agccgatgat
    19021 taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat gataaggagc
    19081 atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca gggcatcgcc
    19141 aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct tacgaacgac
    19201 atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc cagtctcttg
    19261 tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat tggccccatc
    19321 ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga ggatgagaac
    19381 tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac atgcattaca
    19441 aaactgtttt aactttctgt caacaggttt tgtaatggtc actaaaaaac aatctcgcgt
    19501 tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa aagtgctttt
    19561 tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc ttggtattaa
    19621 ccccccgagt ctctacgcgg cttttggcag taaggctggg ttatttagcc gtgtactcaa
    19681 tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg atcgtccggt
    19741 aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc aaaacggcgg
    19801 ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag cgcgtgatat
    19861 tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg ccaggcggca
    19921 tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag ggctttctgc
    19981 gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc tggcagggga
    20041 agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc gggaacgcgc
    20101 ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga attgatcatc
    20161 ttttcgtata acgatagaaa tgaaacattg ttttaattaa ggagtggaaa agatgatcat
    20221 cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg cgattgagca
    20281 tatcaaagca cacgttacgg cagaaacgcc aaagggcaag catgatatcg aaggcaatcg
    20341 tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc gtgcggagta
    20401 ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca tgaccttcag
    20461 cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca tcgcattttt
    20521 gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg ttgtgtttta
    20581 tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg ttcgcaaagc
    20641 agtagtgaag atgctgatgg cgtgatgact tttcgccgta aataactcca ggtttacggc
    20701 gagtttgtga aaagagcgtt ttttgatatt tttttgtgag taaaatttgt aatgcttaga
    20761 cgttcttatt aactcaagga gttcgtcatg ggcaaaatat cagatttgaa ttattctcaa
    20821 cacattacat tagccgacaa ttttaaacaa aaaaatgaag ccttagatac ctggtatgtg
    20881 ggaatgaatg attttgcccg aattgccggg gggcagaata gcagaagcaa tattctttct
    20941 cctagagcat ttttggagtt tttggcaaag atatttaccc tgggttatgt ggattttagc
    21001 aaacgctcca acgaagcggg tagaaatatg atggctcata ttgagttctc atcttattct
    21061 aaagatactg atggcaatga aaaaatgaag ttttacatga ataatcctga aggggagcga
    21121 gcggatttat ctaaggtgaa gatagaaatt acacttgcct cagcttctac taaagggatt
    21181 cgtgaaggac atacagtcat tatatttaaa caatctgatg gttcgactaa tcgttatgaa
    21241 gggaagtctt ttgaaagaaa agatgatagt tcattacacc tgattactaa caaggttctg
    21301 gcgtgttacc aacgcgaagc taacaagaaa atagcgcgtc tattaaataa tcatcagaag
    21361 ttaaataata tacaggagtt aaatgattcg caggagttaa ataattcaca gaagttaaat
    21421 aattcgcagg agttaaataa ctcgcaggtg agttgtaaag gttcagttga ttctacgatt
    21481 acggatttat tagaaaaagc attgaa
//
LOCUS       DAJTSF010000066        21503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000066 DAJTSF010000000
VERSION     DAJTSF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.066"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO335_002621"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO335_002621"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6002961.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO335_002622"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO335_002622"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6002962.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="OO335_002623"
     CDS             complement(3121..3420)
                     /locus_tag="OO335_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6002963.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="OO335_002624"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="OO335_002624"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6002964.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="OO335_002625"
     CDS             complement(4632..5069)
                     /locus_tag="OO335_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR6002965.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="OO335_002626"
     CDS             complement(5115..5456)
                     /locus_tag="OO335_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6002966.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OO335_002627"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OO335_002627"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6002967.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OO335_002628"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OO335_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="OO335_002629"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="OO335_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6002968.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="OO335_002630"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="OO335_002630"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR6002969.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="OO335_002631"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="OO335_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR6002970.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13217
                     /locus_tag="OO335_002632"
     CDS             12687..13217
                     /locus_tag="OO335_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6002971.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13329..13865
                     /locus_tag="OO335_002633"
     CDS             13329..13865
                     /locus_tag="OO335_002633"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6002972.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13973..14797)
                     /locus_tag="OO335_002634"
     CDS             complement(13973..14797)
                     /locus_tag="OO335_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR6002973.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="OO335_002635"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="OO335_002635"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6002974.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="OO335_002636"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="OO335_002636"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR6002975.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="OO335_002637"
     CDS             complement(18729..19202)
                     /locus_tag="OO335_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6002976.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACFEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="OO335_002638"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="OO335_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR6002977.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20352..21350
                     /gene="yiaK"
                     /locus_tag="OO335_002639"
     CDS             20352..21350
                     /gene="yiaK"
                     /locus_tag="OO335_002639"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR6002978.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21362..>21503
                     /locus_tag="OO335_002640"
     CDS             21362..>21503
                     /locus_tag="OO335_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6002979.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5403 a         5489 c         5277 g         5334 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaaatggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgagcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcacccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgcccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggtg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg acaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga tgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacacctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggtaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcattga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgctgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca aaacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgccca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttgatgac agcgtgtggg ccaaaatcca ggcgttatga ggagataagt
    21361 catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa
    21421 ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg gagccgggcg ttgtcgaaat
    21481 cgacggcaag aatatttatg cgc
//
LOCUS       DAJTSF010000067        21277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000067 DAJTSF010000000
VERSION     DAJTSF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.067"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..446)
                     /locus_tag="OO335_002641"
     CDS             complement(<1..446)
                     /locus_tag="OO335_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR6002980.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(501..1352)
                     /gene="manZ"
                     /locus_tag="OO335_002642"
     CDS             complement(501..1352)
                     /gene="manZ"
                     /locus_tag="OO335_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6002981.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1365..2165)
                     /gene="manY"
                     /locus_tag="OO335_002643"
     CDS             complement(1365..2165)
                     /gene="manY"
                     /locus_tag="OO335_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6002982.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2228..3199)
                     /gene="manX"
                     /locus_tag="OO335_002644"
     CDS             complement(2228..3199)
                     /gene="manX"
                     /locus_tag="OO335_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6002983.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3662..5212
                     /gene="yoaE"
                     /locus_tag="OO335_002645"
     CDS             3662..5212
                     /gene="yoaE"
                     /locus_tag="OO335_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6002984.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5216..6814)
                     /locus_tag="OO335_002646"
     CDS             complement(5216..6814)
                     /locus_tag="OO335_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6002985.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="OO335_002647"
     CDS             complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="OO335_002647"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6002986.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8493..9071)
                     /locus_tag="OO335_002648"
     CDS             complement(8493..9071)
                     /locus_tag="OO335_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6002987.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="OO335_002649"
     CDS             complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="OO335_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6002988.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10510..10689
                     /locus_tag="OO335_002650"
     CDS             10510..10689
                     /locus_tag="OO335_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6002989.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10809..11168)
                     /locus_tag="OO335_002651"
     CDS             complement(10809..11168)
                     /locus_tag="OO335_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR6002990.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11530..11874)
                     /locus_tag="OO335_002652"
     CDS             complement(11530..11874)
                     /locus_tag="OO335_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6002991.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12006..13916
                     /locus_tag="OO335_002653"
     CDS             12006..13916
                     /locus_tag="OO335_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6002992.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13974..14669
                     /gene="tsaB"
                     /locus_tag="OO335_002654"
     CDS             13974..14669
                     /gene="tsaB"
                     /locus_tag="OO335_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6002993.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14709..15290
                     /gene="yeaY"
                     /locus_tag="OO335_002655"
     CDS             14709..15290
                     /gene="yeaY"
                     /locus_tag="OO335_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6002994.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15496..17181
                     /gene="fadD"
                     /locus_tag="OO335_002656"
     CDS             15496..17181
                     /gene="fadD"
                     /locus_tag="OO335_002656"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6002995.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17263..18378
                     /gene="rnd"
                     /locus_tag="OO335_002657"
     CDS             17263..18378
                     /gene="rnd"
                     /locus_tag="OO335_002657"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6002996.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18432..19397)
                     /gene="yeaX"
                     /locus_tag="OO335_002658"
     CDS             complement(18432..19397)
                     /gene="yeaX"
                     /locus_tag="OO335_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6002997.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19453..20070)
                     /gene="yeaW"
                     /locus_tag="OO335_002659"
                     /pseudo
     CDS             complement(19453..20070)
                     /gene="yeaW"
                     /locus_tag="OO335_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20412..20978)
                     /locus_tag="OO335_002660"
     CDS             complement(20412..20978)
                     /locus_tag="OO335_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6002998.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21190..>21277
                     /locus_tag="OO335_002661"
     CDS             21190..>21277
                     /locus_tag="OO335_002661"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6002999.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5266 a         5275 c         5465 g         5271 t
ORIGIN      
        1 acgagaagct tataaatttt ttccagatcg tcgatatttc gaacgcggat taacagccga
       61 cgctgttcta attgcatcac caacacgcca tcttccgaca agttcatcgc tttgattcgg
      121 ctatattcaa tccagacatt ggcgaagaaa aaaccttttt gtttaaagat gatcttcgga
      181 atgcggatcc agaatatata aaaacccatc agagccagtg cacttaataa ccatgttgtt
      241 attagcgcgc catgattcgt gacgttgtta tagataagaa tgacaatcaa tccgacgaag
      301 ataacgctat cgatgcgacc acgccggagc aaaggaattg ccagcagggt ggggccgtta
      361 cggcggggca tgatgaactg atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga
      421 atcagtacca ggtccgtgat tgtcattaat cctccagata aaaaaacggg gccaaaaggc
      481 cccggtagtg tacgacagtc ttacagtccc agcaggccgc aagcgtaacc agcgataccg
      541 atgacgaaga agccaacgat gatccacagc gggttaactt ttttgcgcag cagccacata
      601 caagcaaatg tcagcagcag tggtacaagg cctggcatta actggtcaag aatagtttgc
      661 acggtggtga cacgggtctg tccatcctgc ccggtgatgg tcgaaaccac cagcgggatg
      721 ttgacatgtg tccacttgtt aaccaatgcc cccatgacaa acaggccgag gatagacgcc
      781 ccttccgtca gtttttgcag gaagccacca cccatatctt taacgatatc gatacctttg
      841 gagtaaccat acgctacgcc gtagtaacgg gttgccagac gcaccaggtt aaacaggatg
      901 aagaacagca gcggacctaa caggctgccg ctcatcgcga taccggcacc cagtgctgca
      961 aataccggac gtacggttcc ccagaagatc gggtcgccta caccagccag cggccccatc
     1021 aaaccgactt tgataccgtt gatagcaccg tcatcgatct ctgcgccatt agcacgctgt
     1081 tcttccagcg ccagggttac gccgagaatc ggtgcagcca cgaacggctg ggtgttaaag
     1141 aactccaggt gacggcgaat agcttgttta cgagcttcgt tgttctcagg gtagaggcga
     1201 cgaattgccg gtaccataga gaagcagaaa cccagtgcct gcatacgttc gaagttccat
     1261 gaaccctgga agaggttaga acgcaggaat acgccacgaa tatcactttg agtgagtttt
     1321 ttctcggtgg tagtttgagt tgtatcaacc atttcgctca cctgttagtc cagttcgtta
     1381 tcgagatcgt tgttaccagc tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg
     1441 ctaagttgga tgtagagcac tgccataaca gtaccaatca cacccagagc aaccaggtta
     1501 aagttggtga atgctgcggt tacgaagccg aggtagaaga acggcatcag gtagccagca
     1561 cgcatcatgt tgataaccat cgcataacca accaccacga tcatgccccc ggcgatattc
     1621 agaccattgg tcaccacttc cggaatcgca ttcagcatgt tctgtacttc gctggtacca
     1681 acagacagcg caacgatgac ggccggaata gccacacgca ttgcttgcag gaacagagaa
     1741 gaaacgtgga tccaggaaat cgctgtcagg ttgccgttat cagcagcctt atccgcagcg
     1801 tgctggaaag caacggtaat agtacgaacg atgatggtca gtacctggcc cgcagcggcc
     1861 agagggattg ccagtgcgat acctgcacca atgctctgat gacctgcgat aaccagaatg
     1921 gtagaaatga tagaagccag agcggcgtca ggcgcaactg cagcaccgat gttcatccag
     1981 cccagcgcga tcatttccag cgtaccaccg ataataatac cggttttcat atccccaaga
     2041 acgataccca ccagggtaca cgcgattagc ggacggtgaa actgaaattc atcgaggatt
     2101 gatcccatac ctgcgataca ggctacgata aataccagca caatttgaag agtggtaatc
     2161 tccattgtac ttctcctgtt tacgtaagac ttaagtgtga aaactgaatg ataatcaaca
     2221 caatacgtta cttatcgatt ttgctgatca gatccatcat tttcagtttc ggatcggtgg
     2281 aaaccttacg gacttccagc tcaataccgc gtgcattcag ttttttgaac gcctcgatat
     2341 ctttttcatc aaccgaaacc gcgttattca cctgggtttt accctgacgg aatgccatac
     2401 caccgacgtt aacagaggtg attttcacgc cgccttcaac gagacgctct acatctgttg
     2461 ggttggtaaa taacagcatt acgcgttcgc cagcatattt cgggttgttg tagacgcgaa
     2521 tcattttggc aacatcaact acgtgtgctg ttacgcccgg aggtgcaacc tgggtgagca
     2581 gtgtcttacg aacggtatcc gcagccactt catcactaac aacaataata cgggagacat
     2641 tggtttcttt ggtccagcgg gtggcgacct gaccgtgaat cagacggtcg tcgatacgcg
     2701 caaggccaat aaccatgtag tcgtttggcc ccattggttt tgccggagtt ggagccgctt
     2761 ttggtgctgc ggcagcgggt gctggcgcgg ctttttcaac cggtttggct ttcagtgctt
     2821 tcacgccttc acggcctgtt tctactgcca gcgcaaccag ttcatcaaag ctcgggtcat
     2881 catcacgggc cattaacgtt tccacgagca ttggaatgtt aacgcctgca atgacttcat
     2941 aatgctcttt gtcgacgaca atgcggctgg cagcattgaa cgggctgcct ccccatgtat
     3001 caacgagaaa cagcacgcct ttagtggtgt cgagttttgc caactgagcg ttgtattttt
     3061 caatcagcgt ttcggcattt tcacctggaa cgaaatcgat ccagccgacg ttttcctgct
     3121 cgcctaacag catttctgcc gttttaagca actgctctgc agcccaacca tgtgtgccta
     3181 taacaatagc aatggtcact tgctacctcc tttattatcg ttaacacctc aacgtgccag
     3241 atgtattttt gaatcgtcgt ccacaatcga atcgattcag ataagggcta caaccaaaaa
     3301 cccctgcgtt tcgcgattta ttttagatat cgaaaaaatt attttatgtg atgaagatcc
     3361 gtaatttaac tttcgattat cagaaatttc gaaaggtaaa atatccttgt cacattcgtt
     3421 tgcaaaggaa ggtaaatctt tgccaaatca gaggcgtctc tgatatgttt aactcccgtt
     3481 taacaaccat ggagtatagg gcagtagccc gcagtatgga tcgtcaccga cgtcatttca
     3541 gcatcaggcc ttttaacgcc tgcctttctg gcactctatg ccgcaccttt cgtttgcatt
     3601 ttgtcgttac gcctgcatta tttctggcgt cgaatagcta ttccttaagc aggagcttgt
     3661 catggaattc ttaatggacc cctcaatttg ggcggggcta ctcacgcttg ttgttctcga
     3721 aattgtgctg ggtatcgata acctggtctt catcgccatt cttgctgaca aactgccgcc
     3781 aaaacaacgc gataaagcgc gtttgctggg gttatcactg gcgctgatta tgcgtctggg
     3841 gctgctgtcg ctgatttcat ggatggtcac gctgaccaaa ccgctattta ccgtcatgga
     3901 tttctccttc tccggacgcg atctgattat gttgttcggg gggatattct tgctgttcaa
     3961 agcaacaacc gaactgcatg aacggctgga aaaccgcgat catgattccg gccacggtaa
     4021 aggctacgcc agtttctggg tggtcgtcac acagattgtc atccttgacg ccgtcttctc
     4081 gttggatgcg gtaattactg cagtagggat ggttaaccat ctgccggtga tgatggcggc
     4141 ggtagtgatt gcgatggcgg ttatgttgct ggcatcgaaa ccgctgacgc gattcgttaa
     4201 ccagcacccc acggtggtgg tgctctgtct gagcttcctg ttaatgattg gtctgagtct
     4261 ggtggcagaa ggtttcggtt tccacattcc gaaaggttac ctgtatgccg cgattggctt
     4321 ctcgatcatt atcgaagtgt ttaaccagat tgcgcgtcgc aactttattc gtcaccagtc
     4381 gactttgccg ctgcgagcgc gtactgccga tgccatcctg cgtttgatgg gcgggaaacg
     4441 tcaggccaat gttcagcacg atgccgataa cccgatgccg atcccggaag gtgcatttgc
     4501 cgaagaagaa cggtacatga ttaacggcgt actgacgctg gcgtcgcgtt ctctgcgcgg
     4561 gatcatgacg ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat
     4621 ccgcgagcaa ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga
     4681 tgaaatcatc ggtatcgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga
     4741 tgtggcggcg attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat
     4801 caacctgctg ggcgtgctgc gtcgcgctcg cgggagcttt gttatcgtga ccaacgagtt
     4861 tggtgtggta caaggcttgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt
     4921 cccggacgct gacgaaacgc cggaaatcat taccgatggt aacggctggc tggtaaaagg
     4981 cggtacagat ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga
     5041 tgatatcgcg acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg
     5101 cgatgtgatt gatgtagggc cactgcatat caccatcttt gaagccaatg attatcgtgt
     5161 tgatctggtt cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcgttaac
     5221 gtaacgacat aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg
     5281 ccagccattt gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa
     5341 tatgcacgcc atgacggcgc aaatacttcg cctgttcttg cgtttccacg ccctccgcca
     5401 ccagttcaat attcagtctt tgccccagcg cgatgatgat atcggtcacc gtcgagttaa
     5461 ccgcgtcaga acctatagct gcggtaaatg acttatcaat tttcagcacg tcaggacgta
     5521 atgtttcaag ccaggaaaac gagctgttgc cggtgccgaa gtcatcaatc gccagtttga
     5581 cgtttttacg atgcagctcg cgggcaatcc gataatcaac atccagtaag gcatcgcgtt
     5641 cggtgatttc gaggatcagt tgctgaatcg ggtgagcgct aaaccagtac tgattgagat
     5701 cttttatcag cacaccacgg cgaaaatggc tgggtgcgac gttaatgcca acatgaaact
     5761 gactactcat cgggaaaaca tggtgctgac gaatggtttc tgccatcaca tagtgggtca
     5821 gtggcacaat taaatgatgt tcttccgcga taggaataaa cacatccggt gaaatccagc
     5881 cctgacgcgg attattccag cgcagcaaaa tctctacacc aatacattgc tggctgcgcg
     5941 cattaagcaa aggctgacag aacaattcga actcatgttg cgccaggccc agattaattt
     6001 cgcgggaaaa gctcatccgg taagcggtcg ccagccaggc gatatagccc accagtaaac
     6061 ttagcagcac cgccagcggt aattgtgttg gaagatagtg ccatgccagc gccgtagccc
     6121 ccggaccatt tacgttaatg gtaaaaggaa agctttgcga agaaacctga tagttttcat
     6181 tgtcttcagg ttgcggaagg gaatctacca gcccattacc atagagcaaa tgccgtttgt
     6241 ccaccgtcag gctggcgcta ctgatttgcg gcagttgtgg ctcaaggagc attgccgtca
     6301 gtaagtcgat gttaatcatc tctatgaccc cagcattgct actgcccgct gcaggcgtcc
     6361 attgaatcaa aaccggactg cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg
     6421 gttgcggtgc aggaagttca gccagaatgt ccacgacggg cacattgcga taaccaaaaa
     6481 tgctggagca ataaagtgtg ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt
     6541 ttgccgcctg tttacgtaat ggcaaatggg cgacagagca gggcagacca atcagcggaa
     6601 gcaagacttt gctacctgcc tgtaggggaa gcagtacttt atctaattcc tctacagcgt
     6661 gattggcgaa ttgtactacc cgttgttgat ttaagttacg ctgtgaaata aatcgcacgc
     6721 tcagggtcga agcgagcgta acgagtgcgc aaatcgtaca aacaatcatt cgattacggc
     6781 gataggtttt aatgatccgt tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg
     6841 acaaacgcct gacgcaacag tggaagtgta gtggggaata ttacagcaga cgagaaagcg
     6901 ggtataaatt cgcccatccg ttgcagatgg gcgagtaaga agtattagtc acactggact
     6961 ttgattgcca gaccaccgcg tgaggtttcg cggtatttgg cgttcatgtc cttaccggtt
     7021 tcgtacatcg tttcgatgac cttatccagc gagacgcgcg gtgcactggt gcggcgcaga
     7081 gccatccgtg cggcgttaat cgccttcaca gaggcaatgg cattacgctc aatgcacggc
     7141 acctgaacct gccctgcaac cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt
     7201 tccgccgcca cgcaaacctg ttccgggcta ccgcccagca gttctgcaag acccgcagca
     7261 gccattgaac aggcaacacc cacttcgccc tggcaaccaa cttccgcacc ggaaatagag
     7321 gcgttcattt tatacaatgc accaatcgcg cccgctgcca taaagtaacg ggtatagatg
     7381 tctgggctga ccgattcaat aaagtggtca tagtaagcca gcactgccgg aacgataccg
     7441 caggcaccgt tggttggcgc agttaccaca cgaccaccgg cggcgttttc ttcgttaact
     7501 gccagcgcaa acatgtttac ccagtcaatg acattcatcg gatcgttaga cagtttatcg
     7561 ctggaaacta acatccggcg cagggcagac gcacgacgtg gcacgcgcag cgggcctggc
     7621 agtacacctt cggtgttcat cccgcgatcg atacatgcct gcatggtttg ccagacatgc
     7681 gcgaaatact cgtcgatctc tttcttgctg tgcagcgcca gttcgttctg catagcgaga
     7741 ccagacagcg aatagccggt ttcattacag tacgcgagca gttcggtggc agatttgaac
     7801 ggatacggca cgctcacttc gttggcagca tcctgaccaa agtgttcttc atcgacgata
     7861 aaaccgccgc cgatggaata ataagttttg ctgtagacga cttcatcgcc gttataggcg
     7921 tggatttgca taccgttttc atgcagcggc aggttgccgt tatgaaaacg catcccgttg
     7981 tcgcgcggga aatccacttc atgccgtccc tgtgccagca gcagacgttc gcgctctttt
     8041 acgtcgcgaa taaaaccggg aatactgtcg atatccacgg tggcaggttc gttacctgca
     8101 agacccataa taatggcgat atcggtgtgg tggcctttac ccgtcagcga cagtgaacca
     8161 taaacgtcca cggcaacgcg agtaacgcta tccagtaagc ctttttcgac cagatcatcg
     8221 acgaactgtt tacccgcctt cataggccct acggtatggg aagatgaggg accaatcccc
     8281 accttaaaca tgtcgaatag actaatcacg ataatactcc tgacaaggtg actggacttc
     8341 cagtaacgaa ctaataacag cgcatagtgt aagagggaac gcggcgactg gcttaactat
     8401 tcacatgaat taaactaatc aaaaaccgcg atttaactaa tattgcgatg attgtctcaa
     8461 gtagtgtttt gtagatgtaa agataagtat agtcaggggg tcacaccaat ttgcagcgcc
     8521 agctcacgaa ttatgcctgc ggtcattccc catacaaaat actgttcgta ccaggacagc
     8581 catacccgat gtgaatcgcc acggcggtag atatctaaag ggtgataacg acccagatgt
     8641 aatgcctggg cgagcggcat ttcaaacacc gccgagactt catcttcact ggcgcgatac
     8701 ggcagatcgg gcgggataat gccgaccact ggggttactt gatagccagt tacgctatcg
     8761 acgggcggca gcacgccgat aacttcaacg gcggaaggcg gtatagcgac ctcttcttca
     8821 gcttcgcgca gcgcggcggc gataactgat gcgtccgtgt catcgactgc acctccaggg
     8881 aatgccactt gtccagcgtg tttacgcaga tgaattgaac gctgagtcag caacaacccc
     8941 ggttgcggtc gacggacgat ggggattaac acggcagcct gacgatgatt taggggttcc
     9001 cggttaattt gcgggcgcaa aagttgaaag cgagataaaa aatcatcaag cgtcaggcta
     9061 cggtattcca cgtcttactt ctccagttgg cgtaatatct tattaacttt atcaaaagtt
     9121 tcctgatatt ccgcttcttc ctggctatcg gcgacaattc caccgcccgc agagcagtat
     9181 atttgtccgt taatggcagt cagcgtgcgg atagtaatgc tggtatccat gttgccgcaa
     9241 aagctcaaat agccaatgct gccgcaccag gcattacgtc gctgcggttc cagttcgtcg
     9301 ataatttcca tagcccgtac tttcggagcc ccggttattg agccaccagg aaaagctgcg
     9361 cgcagcagat cgctggcgtg taactgttct ggtagtcgcg ccgttatagt gctgaccaga
     9421 tgatgcacgg cagggaaggg ttccaccacg aagagctctg gtacttttac cgaaccggct
     9481 acggcaacac gaccgatatc attacgcatt aaatcgacaa tcatcagatt ttcggcacga
     9541 tctttcgctg agttcgccag tttttctgct tgtttgctat cttcctgagg atcgggcagg
     9601 cgtggtagcg tgcctttaat cgggcgagtc tggatttcac tattatcaca aagaataaac
     9661 cgctctggcg aaaggcttaa aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc
     9721 gcgcggttgg cctgattaag ctgaaggaat gcctgccatt catcgccaga ataggtcgca
     9781 tgaaaacgct gggcgagatt cacctgatag caatcaccgc tgtgcagata ttcctgtacc
     9841 tggcgaaatt tttcgccgta ctgctcgtgg gtcatattgg attgccagtc ggaagtgagc
     9901 gtgaaatctt cctgcggcga gaattgctgg ctttccagcc aggcccgacg ggcattgaca
     9961 tcattatgac tcagcaaaga aactgtatga cgctggtggt cgacaatgag cgcccaatcg
    10021 tagataccca ctgccatatc cggcagaacg atatcttgtt ccgcaatttc tggcagtgac
    10081 tcaaaacggc ggcccagatc gtagccaaac aaccccagtg cgccgccctg aaatggcaaa
    10141 tcttcgttat gcgttgggcg aatgtctgcg cgatccagca cctgctggag cacctgtagc
    10201 gggtcatcag tggtcgttgt gcgtttttcg ctttcactaa caacggtttc tttacctaaa
    10261 gtggttaaag tgcaaatcgg atcggcgacc acaatatcaa agcggctata cggatgatcg
    10321 gcatagccgg agtgtaaaag catcgcccac ggcaggtggc ttaagcggga gaaataaaat
    10381 tcagcggcgt cctgacgcca gggtaaagta atcacagtgg gagataacgt cttcatcagt
    10441 cctgactcta ctggctatgt gccgggtagc atgaaataat tagcgccaac aatttagcag
    10501 gagttaacaa tgtttgcagg tttaccttca ctcatccatg aacagcagca aaaagctgtc
    10561 gagcggatcc aggaactgat ggcacaaggg atgagtagcg gccaggcaat cgcgctggtg
    10621 gcagaagagc tgcgtgccaa ccactccggc gagctaatcg tggcgcgttt tgaggatgaa
    10681 gacgagtaac acactaatcc gtatcgattt cgttatagaa gccgatgatc atttgattgt
    10741 gagaaaaagg acgcttttta tatgtgatta aaagcgtcct gacccattta tgctgatata
    10801 aatttaactc agaccagtgc ttttagttct tcctgcatat atgttgaaaa gtattcagga
    10861 tttttaatca cagagcgttc acctgtctcc attttttttg cccatccaac cattttttcc
    10921 gtgaacccag ggttccagcc ttcctggtca gctcgcgccg taatatcagc ggcactcgcg
    10981 ggtactccca gttcttttaa atatttaaag attcccttcg ccgtgctttc attcatcgaa
    11041 cttggtgttg gcgctgatac atccatggca ccaatgaaat ccagggcttt atctattaca
    11101 gcaggcatat tcttatcctt aaaattaacc atgttatcga taaggctata tatgacctta
    11161 atttgcatct aatatcaaag ggattataac gatatggcac gagttaacct gttgacattc
    11221 aggtgtttta aaggtagcag ctcatggtgt gttatccagt aagatggaga aaattaacgg
    11281 tcagcccgca cgctataacc agattgttca gctaagaaag aaaaccagag ttgctaatca
    11341 aagggcgaac acaacggcat ggttatgttt atttatatag ttagttttat agataaaatt
    11401 gaaattataa atataaataa tgaattagca gaagcatcaa cagtaatatc ttattctctg
    11461 gtcgatttat gtaaaaccga ccagtattat cgataaatta tcgtctgact tattttgctt
    11521 cgataaagct tacaccgcag ccacaatctt aatttcaact ttatacttcg ggttcatcaa
    11581 acccgcttgt accgtgcagc gcaccggcgc atgacccgcg acaacccagg catcccacgc
    11641 tttattcatc gccgcgaagt cgtttttatc ggccaggaaa atggtggcat ccagaatgct
    11701 cgatttattg ctgccctgtt tttccagcac ggcgtcaatc tgtgccagcg tgttggcggt
    11761 ttgctcaaag gcatcggcgt cgaggttttc cggtacacca gtgtagtaga gcgtgttgtt
    11821 gtggattact acatcagacc agcgggcttc agcatcgata cgaacgatag tcatcatttt
    11881 gtcctcatta tacttccatt ttcgtgctgc aaagactgcc acaatgtgag gttgccgtca
    11941 ccacttcaac tggcgaaagc gccctcatcc tgacataatc cctgttcaaa tcaacagggg
    12001 gtagtgtgac ggacgatttt gcaccagacg gtcagctggc gaaagcgata ccaggcttta
    12061 agccgcgaga accacagcga cagatggcgg tagccgtcac ccaggcgata gaaaaaggcc
    12121 agccgctggt ggtggaagca ggaaccggta cgggcaaaac ctacgcttac ctggctcctg
    12181 cgctgcgggc gaaaaagaaa gtcattatct cgaccggctc aaaagcgttg caggatcagc
    12241 tctacagccg cgatttgcca acagtctcaa aggcattgaa atatacgggc aacgtggcgc
    12301 tgctgaaagg gcgctcaaac tacctctgcc tcgaacgtct cgaacagcag gcgctggcgg
    12361 ggggcgatct gccggtacaa atcttaagcg atgtgatcct gctgcgctcc tggtctaatc
    12421 aaacagtcga tggtgatatc agcacctgcg tcagcgtggc ggaagattca caggcgtggc
    12481 cgctggtcac cagcaccaac gacaactgtc ttggcagcga ctgcccgatg tataaagatt
    12541 gctttgtggt caaagcacgt aaaaaagcga tggacgccga tgtggtggtg gtaaaccatc
    12601 atctctttct ggcggatatg gtggttaaag agagtggatt tggcgagttg atccctgaag
    12661 cggacgtcat gatcttcgac gaagcccacc agctaccgga cattgccagc cagtattttg
    12721 gtcagtcact ctccagtcga caactgctcg acctggcaaa agacatcacc atcgcctacc
    12781 gcaccgaatt aaaagacacc cagcagttac aaaagtgcgc tgatcgtctt gcccagagtg
    12841 cgcaggattt tcgtctgcaa ctcggtgagc caggttatcg cggtaacctg cgtgagctgt
    12901 tagctaatcc gcaaattcag cgggcatttt tactgctcga tgacaccttg gaactttgtt
    12961 atgatgtggc gaaactgtca ctggggcgtt ccgccttgct ggatgcggca tttgagcgcg
    13021 ccacgttgta tcgcacacgg ctgaagcggc taaaagagat caatcagccg ggctacagct
    13081 actggtacga atgcacttcg cgccatttta ctctggctct cacgccgctc agcgtggcgg
    13141 ataaattcaa agagttaatg gcgcaaaagc ccggtagctg gatcttcacc tcagcaacgc
    13201 tgtcggtgaa cgacgatctg catcatttca cctcgcggct tgggatagaa caggcggagt
    13261 cgttgctatt gccaagcccg tttgattaca gccgccaggc gttactctgt gtgccgcgca
    13321 atctgccgca aaccaaccag ccaggttctg ctcgccagtt agcggcaatg ctgcgaccga
    13381 tcatcgaagc taacaacggt cgttgtttta tgctttgtac ctcgcacgcc atgatgcgcg
    13441 atctggccga gcagttccgc gctaccatga cgcttcccgt tttgttgcag ggggaaacca
    13501 gtaaaggcca actgttgcag cagtttgtca gtgccggtaa tgcgcttctt gtggcaacca
    13561 gtagtttctg ggaaggggtg gacgtgcgtg gcgatacatt gtcattggta attatcgaca
    13621 aattgccgtt tacctcgccg gatgatccac tgttaaaagc gcgcatggaa gattgtcgtt
    13681 tgcgcggtgg cgacccgttc gatgaagtgc aactaccaga tgccgtcatt actctcaaac
    13741 agggggtagg tcgactgatt cgcgacgccg acgatcgtgg cgtgctggtg atttgtgact
    13801 atcggctggt gatgcgtcct tacggcgcga cgtttctcgc cagtctgccg cccgcgccac
    13861 gcacccgtga cattgcccgt gcggttcgtt tccttgcgat accatcctcc aggtaatttg
    13921 ttacggagta tggtatgatg cgcgcctttt tattgatcta agcacgaaga cccatgcgaa
    13981 ttctggctat cgataccgcg acagaggcct gctctgtcgc cctgtggaac gacggtactg
    14041 tcaacgctca ttttgagctt tgccctcgtg aacatactca acgaatctta ccgatggtgc
    14101 aggatatcct gaccaccagc ggaacttccc tgactgatat taacgctctg gcttacgggc
    14161 gcggccccgg tagctttacc ggcgtgcgca ttggtattgg catcgcgcaa gggctggcgc
    14221 ttggcgcgga attaccgatg attggcgtct ccacgctaat gacgatggcg caaggggcgt
    14281 ggcgcaaaaa cggcgcaaca cgcgtgctgt cagccattga tgcgcgaatg ggcgaagttt
    14341 actgggccga atatcagcgt gatgaaaacg gtatctggca cggtgaagaa accgaagccg
    14401 tactcaaacc cgaactcgtt catgaacgaa tgcaacagct ttccggcgaa tgggtgacgg
    14461 tagggacggg ctggcaagcc tggccggatc tcggtaaaga gagcgggctg gttttgcgcg
    14521 atggcgaagt gttactcccc gctgcagaag atatgctgcc gattgcttgt cagatgtttg
    14581 ccgagggtaa aacggtggcg gtggaacatg ccgagccggt ttatttgcgt aacaacgtcg
    14641 catggaagaa acttccgggc aaagaatgaa tcttaagagt atgccctgag aaaaaaagga
    14701 gtcgcatcat ggcggttcaa aagaatgtta tcaaaggcat actggcaggt acgtttgcgc
    14761 taatgctgag cggttgtgtc actgtgccgg acgccattaa aggcagcagt accacgccgc
    14821 aacaagattt agttcgggtg atgagtgcgc cgcagctgta cgttggtcag gaggcacgct
    14881 ttggtggcaa agtggttgcg gtacaaaacc agcaagggaa aacccgcctg gaaattgcta
    14941 ccgtaccgct ggacagcgga gctaggccga cgctgggaga accttctcgc ggtcgcattt
    15001 atgccgatgt gaacggtttt ctggacccgg tggatttccg tggacaactg gttacggtag
    15061 tcgggccaat caccggtgcg gttgacggca aaatcggcaa tacgccctat aaatttatgg
    15121 tgatgcaagt aacgggttac aaacgttggc atttaaccca gcaggtgatt atgccgcctc
    15181 agccgattga tccatggttt tatggcggtc gtggctggcc ctatggctac ggcggatggg
    15241 gctggtataa tcccggcccc gcgagagtac aaacagttgt aaccgaataa ttgcttgttt
    15301 ttaaagaatg cagaaacagc ggctggtccg ctgtttctgc attcttacgg taaagataaa
    15361 aataaatagt gacgcgcttc gcaacctttt cgttgggtaa ttatcaagct ggtatgatga
    15421 gttaatatta tgttaacggc gtgtatatca tttggggttg cgatgacgac gaacacgcat
    15481 tttagaggtg aagaattgaa gaaggtttgg cttaaccgtt atcccgcgga cgttccgacg
    15541 gagatcaacc ctgaccgtta tcaatctctg gtagatatgt ttgagcagtc ggtcgcgcgc
    15601 tacgccgatc agcctgcgtt tgtgaatatg ggggaggtga tgaccttccg caagctggaa
    15661 gaacgcagtc gcgcgtttgc cgcttatttg caacaagggt tggggctgaa gaaaggcgat
    15721 cgcgttgcgt tgatgatgcc taacttattg caatatccgg tggcgctatt tggcattttg
    15781 cgtgccggga taatcgtcgt aaacgttaac ccgttgtata ccccgcgtga gcttgagcat
    15841 cagcttaacg atagcggcgc atcggcgatt gttatcgtgt ctaactttgc ccacactctg
    15901 gaaaaagtgg ttgataaaac cgccgttcag cacgtaattc tgacccgtat gggcgatcag
    15961 ctatctacgg caaaaggcac ggtagtcaat ttcgttgtta aatacatcaa gcgcttggtg
    16021 ccgaaatacc atctgccaga tgccatttca tttcgtagcg cactgcataa cggctaccgg
    16081 atgcagtacg tcaaacccga actggtgccg gaagatttag ccttcctgca atacaccggc
    16141 ggcaccacag gtgtggcgaa aggcgcgatg ctgactcacc gcaatatgct ggcgaacctg
    16201 gaacaggtca acgccaccta tgggccgttg ctgcatccag gtaaagaact ggtagtaacg
    16261 gcactgccgc tgtatcacat ttttgccctg accattaact gcctgctgtt tatcgaactg
    16321 ggtgggcaga acctgcttat cactaacccg cgtgatattc cagggttggt aaaagagtta
    16381 gcgaaatatc cgtttaccgc tatcactggc gttaacacct tgttcaatgc gttgctgaac
    16441 aataaagagt tccagcagct ggatttctcc agtctgcatc tttccgcagg cggtgggatg
    16501 cctgttcagc aagtggtggc agaacgctgg gtgaaactga ccggacagta tctgctggaa
    16561 ggttatggcc tgaccgagtg cgcgccgctg gtgagcgtta acccgtatga tattgattat
    16621 catagcggta gcatcggttt gccggtgcca tcgacggaag ccaaactggt ggatgacgac
    16681 gacaacgaag taccaccggg tcaaccgggt gagctttgtg tcaaaggacc gcaggtgatg
    16741 ctgggttact ggcagcgtcc ggatgctaca gatgagatca tcaaaaatgg ctggttacac
    16801 accggcgaca tcgcggtgat ggatgaagaa ggattcctgc gcattgtcga tcgtaaaaaa
    16861 gacatgattt tggtttccgg ttttaacgtt tatcccaacg agattgaaga tgtcgtcatg
    16921 cagcatcctg gcgtacagga agtcgcggct gttggcgtac cttccggctc cagtggtgaa
    16981 gcggtgaaaa tcttcgtagt gaaaaaagat ccatcgctta ccgaagagtc actggtgact
    17041 ttttgccgcc gtcagctcac gggatacaaa gtaccgaagc tggtggagtt tcgtgatgag
    17101 ttaccgaaat ctaacgtcgg aaaaattttg cgacgagaat tacgtgacga agcgcgcggc
    17161 aaagtggaca ataaagcctg agcgttaagt cagtcgtcag acgccggtta atccggcgtt
    17221 ttttttgacg cccactaaag agaaaacaat ttgaattacc aaatgattac cacggacgat
    17281 gcgctggctt ctttgtgtga agccgtccgt gtctttccgg cgatagccct ggatactgaa
    17341 tttgttcgta cgcgcactta ttacccgcag ctggggttga ttcaactttt cgatggcgag
    17401 catctggcgc taattgatcc tctcgggatc accgactggt caccgctgaa agcgatcctg
    17461 cgcgatccgt ccatcacaaa atttctccat gcaggcagtg aagatctgga agtgttcctc
    17521 aatgtctttg gcgaattacc acaacccttg attgacacgc aaatccttgc tgccttctgc
    17581 ggacgcccga tgtcatgggg tttcgcttcc atggtggaag agtattccgg cgttacgctg
    17641 gacaagagtg aatcgcgcac cgactggctg gccagaccgc tgaccgaacg tcagtgtgaa
    17701 tacgcagcgg cggatgtctg gtatctgtta ccgatcaccg ccaagcttat ggtagaaacg
    17761 gaggcctccg gctggctacc tgcggcgctg gatgaatgcc gcctgatgca aatgcgtcgt
    17821 caggaagtcg ttgcgccgga agatgcctgg cgtgatatca ccaatgcctg gcagttacgc
    17881 acacgccaac tggcctgtct gcaactgtta gccgactggc gactgcgcaa ggcgcgagag
    17941 cgtgatctgg cggtgaactt tgtcgtgcgt gaagagcatt tgtggtcggt cgcgcgttat
    18001 atgccgggaa gtttaggcga actggacagc ctgggtttat ccggtagcga aatccgcttt
    18061 cacggtaaaa cgctgctggc gctggtggaa aaagcgcaga cattgccgga agatgcctta
    18121 ccgcagccga tgcttaacct gatggacatg cctggttatc gtaaagcgtt taaagcgatt
    18181 aagtcgctga ttactgacgt gagcgaaacg cataagatca gcgccgaatt gctggcatcg
    18241 cgtcggcaaa tcaaccaact gctgaactgg cactggaaac tgaaaccgca gaacaatttg
    18301 ccggagctga tttccggctg gcgtggtgag ctgatggcgg aagcattaca caatttattg
    18361 caggaatatc cgcagtaaaa tcttccgaag ccggactggg cgcgctcagc gccacatccg
    18421 gctttggcaa actacaaatc caacaccagg cgtttaccct tcgcacgcga acaacagatc
    18481 aacatacttt gctggctggc acgctcttca tcgctaaaat actgatcccg atggtcagct
    18541 tcaccttcca gtattgccgt ttcgcaggtt ccgcataccc cttcacgaca taaacattcc
    18601 actttcgcgg ctttattatt ttcaataacc tgcaaaatag tcatctcttc cggcaccaca
    18661 aactcttttc cggaacgggc aagcaccagg gtaaatgcat cgccggtttt gtcttcgata
    18721 gcaaattgct caaagtgcag tgtatcggcg gcgatgtcca gacgcgcagc ttcacttctt
    18781 accgcttcaa ttagcgcctc ggggccacag gtataaacgt gtgtgccagg ttcgatatcc
    18841 gccaataatc gcgctaattc cagtcgtgtt ccggttgatg aatgatgcaa atggactttc
    18901 tcagcctgcg gatgctggac tagctcagca cgaaatgcgc aactttctgg atttcgcgag
    18961 cagtaatgta gctgccagtc gatgtcgctg tgttgcaact ctgccatgtg cgacaggaaa
    19021 ggggtgatac caataccgcc cacgataaac agatgctttc tggctgaggg aattagcgca
    19081 aacaggttat taggcgttga gatcgttaac cgatcgccca cttttacctg ctgatgcaaa
    19141 aagcgggaac cgccgcgcga gttttcttcc agtcgaacgg caatctgata actagaggtg
    19201 ttatgcggtg aactcagtag tgaatacgca ttgctgtact ggttatcacc atcgctcatc
    19261 tgcactagga tgtgacttcc tccggtgaac gcaggcagcg gtttgccatc ggttgccacc
    19321 agcgtgaagc gtttcacctg ttcggtaagg ggttcaacct ggctcacctg tacttcaaac
    19381 atttgatagt ctgacattgc tgtactccat caaaaatcag cccagcggcg gaaaataccg
    19441 ccgccgatgt cattagtcct taaacacctg cgccagcaga ttatggaaat gggcgatacc
    19501 atgttcggaa atgccactac cgctactgtc ggccatgatg cgcccctgac cacgatagcc
    19561 acgcgatttc agccctttct gtacgctttc aaccagacgt aaatcttccg gatggaacac
    19621 atcgcgatac cactcaatca gcgatttttg ctcgtcggtt aactcttcat tggtgaagta
    19681 aatatcgtag ttttgcaggg tagtttcaga atccaccggg aattcataaa tgaccgtcat
    19741 catccctttg atcggggtga cgttcagcat cgtgcacggc cacagccaga aaccgtggaa
    19801 tgccgcatcc gtaccctctt caaatttaaa cgactgttcg gacggtttgg cgaaaccgta
    19861 ttgcagcgtc cagttaccgt gcatggtgtg ccagtaacga tcaacctgta cggagtcgga
    19921 gaaacctaga tgcgccggac cacagtgata gcactcgaga tagttatcga caatgttctt
    19981 ccagttggca ggcgtgcggg tggtaaagcg ggccgctagt ttcagattgt ggacttccgg
    20041 gcaggcttcc agcactttcg cgcccagacc ggtgtattga ttcacttgaa gtacgaaaaa
    20101 aaaccgggag gacattggat cattcgggtt ctgatgggat tagatttggt ggggcttgca
    20161 agcctgtagc gcaaatttta gtcgttaatc aatgaaacgc gaaagatagt aaaaaattgc
    20221 ttttgtttca ttgaaaatac gaaaaacaaa aacactgcaa atcatttcaa taacagcttc
    20281 aaaaaacgtt caaaaccgat aacaaccagg ctgtaaccaa atgactcata tcacaaatca
    20341 gctcatgccg tttaggtatg ttacatgtgt gattatgtga ggtgaagtat gttttagctg
    20401 gttcatggtt gttatacggc tttttttaca tcttgtggtt cttgtgaagg agctacaaga
    20461 ccttcctgtt cataaatacc atactttgac aaaatctttt tacgagtatc tgttggtaat
    20521 gattcaagca ttttggctgt ttgtaaggtt tgctgcgaaa taccttgcaa atcagcctgg
    20581 atggaatcat cggaagaaaa aatctcaggc ataagagctt caaatccttt ctttctgaac
    20641 aactcaatcg cagaatgcag ttccttgtcc tctaacaatt cgacaattgc gatcagttta
    20701 gttttcaatg tgctgcggtc aggttgagaa tttttattct cttgattatt tttatgctca
    20761 accattggca tgctctgact ttggataaac cactctattg gacgccctgt tgcgtaggct
    20821 atttgtgcta atcgatctaa agacgggtat gtcttgccag acaggtagtt tctgataacc
    20881 ccatcagaaa gtccgcactt agctgcaaac gcccggcttg attctccatt cattgcctct
    20941 tcaaggcgtt ccttgaaacg cattatttcc gggggcataa aaatgcgctc tgcatttttg
    21001 ctttcttttt cacctgtcat gcccaatctc tctgttatat aaagaatttt acagaattct
    21061 ttgatttgca ctggttaaaa taaaaatgcg catagctttt gaattgagca tttttctgcg
    21121 ctatcgtttt tcccgatagc ggtagccggt atcagttacc gaaaacgtta agagagtaag
    21181 gatcgcagaa tgtcagcaaa aaaccaagac tggcacgcag agcagataaa ggctgctgtt
    21241 cgtatgaagg ggaagacact atctcaatta tccagag
//
LOCUS       DAJTSF010000068        21253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000068 DAJTSF010000000
VERSION     DAJTSF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.068"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            18..791
                     /locus_tag="OO335_002662"
                     /pseudo
     CDS             18..791
                     /locus_tag="OO335_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(851..2149)
                     /gene="eno"
                     /locus_tag="OO335_002663"
     CDS             complement(851..2149)
                     /gene="eno"
                     /locus_tag="OO335_002663"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6003000.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OO335_002664"
     CDS             complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OO335_002664"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6003001.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OO335_002665"
     CDS             complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OO335_002665"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6003002.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4989..7223)
                     /locus_tag="OO335_002666"
     CDS             complement(4989..7223)
                     /locus_tag="OO335_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR6003003.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OO335_002667"
     CDS             complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OO335_002667"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6003004.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8629..11385
                     /gene="barA"
                     /locus_tag="OO335_002668"
     CDS             8629..11385
                     /gene="barA"
                     /locus_tag="OO335_002668"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6003005.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OO335_002669"
     CDS             complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OO335_002669"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6003006.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12978..14318)
                     /locus_tag="OO335_002670"
     CDS             complement(12978..14318)
                     /locus_tag="OO335_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6003007.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OO335_002671"
     CDS             complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OO335_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6003008.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16106..16555)
                     /locus_tag="OO335_002672"
     CDS             complement(16106..16555)
                     /locus_tag="OO335_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6003009.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OO335_002673"
     CDS             complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OO335_002673"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6003010.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17355..17684)
                     /locus_tag="OO335_002674"
     CDS             complement(17355..17684)
                     /locus_tag="OO335_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6003011.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OO335_002675"
     CDS             complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OO335_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6003012.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18919..19767
                     /gene="queF"
                     /locus_tag="OO335_002676"
     CDS             18919..19767
                     /gene="queF"
                     /locus_tag="OO335_002676"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6003013.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19879..21243
                     /gene="ppnN"
                     /locus_tag="OO335_002677"
                     /gene_synonym="ygdH"
     CDS             19879..21243
                     /gene="ppnN"
                     /locus_tag="OO335_002677"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6003014.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5228 a         5671 c         5067 g         5287 t
ORIGIN      
        1 ttgcagccca accaactatt gtcccacaat tacagcaaca agttacgggt cttacgagta
       61 gcttaaactc acaagaaaag aaagaactga cgcacaagtt agaatctatt ttcaataata
      121 cccaagtgca aattgctgta ttaattgttt ccacaaccaa agatgagaca attgaacaat
      181 atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac
      241 tgatcgttgt tgcctagtcg gatcgcactg tccgcatcca ggtaggttat gggctggaag
      301 aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta
      361 aacaacaaaa attagctaag ggattagagt tagctataat cgctttgaat aaccaactca
      421 cttcacaaca ccaatatccg gctaatcctt cagaaagtga atcaacgtct tccactgatc
      481 attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc tggttttttc
      541 atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctgtatttca gcaatccatc
      601 ttttagattt attcctgttc tccgataaaa ctttttccat tgctgtattt tccttctttt
      661 ttactttcac catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatctg
      721 gtagaagtta tcattcagac aacagcggtt ccgctggagg ctcaagtggc ggcgcatccg
      781 gccgctggta actccaccat aaaaaatgcc agcccgaagg ctggcattat caaatcagat
      841 aaagtcagac ttatgcctgg cctttgatct ctttacgacc gttgtacggt gctttttcgc
      901 ccagagcttc ttcgatacga atcagctggt tgtatttagc aacacggtca gaacggctca
      961 tagaaccagt tttgatctgg cctgcagcag taccaacagc cagatcagcg atggtagcat
     1021 cttcagtttc accagaacgg tgagagataa ctgcagtgta gccagcatct ttcgccatct
     1081 tgattgcagc cagagtttcg gtcagagaac cgatctggtt gaatttgatc aggatggagt
     1141 tagcgatacc tttttcgata ccttctttca ggatcttggt gttggttacg aacaggtcgt
     1201 caccaaccag ctggattttg tcgcccagaa ctttggtctg gtatgcgaaa ccgtcccagt
     1261 cagattcgtc cagaccgtct tcgatagaaa cgatcgggta ctgtttggtc agttcttcca
     1321 ggaagtgagt gaattcttca gaggtgaacg ctttgttgcc ttcgccagcc agaacgtatt
     1381 taccatcttt gtagaattca gaagctgcgc agtccatcgc cagagtgatg tctttgccca
     1441 gttcataacc agcagcttta acagcttcag cgataacagc cagagcttca gcgttggaac
     1501 ccaggttcgg cgcatagcca ccttcgtcac caacagcagt gttcatgcct ttcgctttca
     1561 gaacttttgc caggtgatgg aaaacttcag aacccatgcg gatggcttct ttcacagttt
     1621 tcgcgccaac cggctgaatc atgaattcct ggatatcaac gttgttgtca gcgtgctcac
     1681 caccgttgat gatgttcatc atcggaaccg gcatggagta tttgcccgga gtgccgttca
     1741 gttcagcgat gtgctcgtac agcggcatac ctttagcagc tgcagcagct ttggcgttag
     1801 ccagagatac agccaggatt gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca
     1861 ggtcgatcat gatcttgtca atgccagcct gatctttagc atctttgcca atcagcgcct
     1921 gagcgatcgg gccgtttacc gcagcaacag ctttggttac gcctttaccc aggaaacggg
     1981 atttgtcgcc atcgcgcagt tccagagctt cacgggaacc agtagaagca cctgacggag
     2041 cagctgccat accgacgaaa ccaccctcca gatgtacttc ggcttcaaca gtcgggttac
     2101 cacgggagtc gatgatttca cgaccgatga tttttacgat tttggacatt aggttttcct
     2161 caagtcacta gttaaactga aactccagac aaacaacgcg tacccaaggt acgcgttgcc
     2221 gttctaactt ttttacttac ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa
     2281 agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt aaactccgga tggaactggc
     2341 aagccacgaa ccacgggtga ttcggaactt cgatgatctc gaccaactga tcatccccgg
     2401 aacggcccgc aacgcgcaga cctgcatctt caatctgttt caacagcatg ttgttgactt
     2461 cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt gtatagctgg cgaaccaggc
     2521 tatcgtcaac caactggcac tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct
     2581 tctcgctacg aacttcaacg ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa
     2641 ccgggtactt acagtctggc acaaattctg tagagttggc gttctccatg ttggcaacat
     2701 ggcgagcgta atcaattaac gccacctgca tacccaggca aatgcccaga taaggaatat
     2761 tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc tacgccacga tagccgaaac
     2821 cgccaggtac gaggattgcg tccagacctt taaggatttc aacgccgcgc gtttcaacat
     2881 cttgtgaatc gatcagtttg atgttgacgc tgacacgatt cttcagccca ccgtgtttca
     2941 gtgcttcgat cactgattta taagcatccg gcagttcaat gtacttgccg accataccga
     3001 tggtgacttc acttaccgga ttcgcttctt cgaagataac ctgttcccat tcggacagat
     3061 ttgcttccgg gcagtttaag ctgaatcgtt tacaaatata atcgtccagc ccctgagatt
     3121 tcaacaggcc cggaatttta tagatggaat cgacgtcttt cagagaaata accgcttttt
     3181 ccggaacatt acagaacaat gcaatcttcg cacgttcgtt cgccggtaca gcgcgatctg
     3241 aacgacaaat caggatgtca ggctggatac cgatggagag cagctctttt acagagtgct
     3301 gagtcggttt ggttttgact tcaccagacg ctgccatgta cggcaccagc gtcaggtgca
     3361 taaacagagt gtgctcacgg ccaatttcaa cagccatctg gcgaatagct tcgaggaacg
     3421 gcaaggattc gatatcacct actgtaccgc cgatttctac cagtactacg tcatgacctt
     3481 cgccaccttc cagcacgcgc tctttgattg cgttagtgat gtgcggaata acctgcacgg
     3541 ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac gtcagagtag atacgacccg
     3601 tggtgaagtt gttgcggcgg ctcattttgg tacgaatgaa acgctcgtag tgccccaggt
     3661 ccaagtcggt ttcagcgccg tcttcagtaa cgaacacttc cccgtgttgg attgggctca
     3721 tagtacctgg atcgacgttg atgtaaggat ccagtttcat gatggtcaca ttgaggccac
     3781 gggcttcaag aatggctgcg agggaggctg cggcaatgcc tttacccaga gaggatacga
     3841 ccccgccggt cacaaaaata tagttcgttg tcatgctgaa cctgagaagt taggttgaaa
     3901 agacgatgga ataaccaaga cgggaaagca gtatacccga acatgacctg tgccacaaac
     3961 tttcattatc cctcctcttc gccagcgcac tattgaaatc aggagtgaga aaatagcccc
     4021 tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatg
     4081 gcgcttgacc gcgtaattcc cttagagatc aatttcctgc cgttttacct gttgccagac
     4141 ttcttccatt gtttcgaggt caacacctgt catttccagt ccacgcgcgg caacaatacg
     4201 ctccacttcg cgaaaacgac gctcgaattt ttcgttcact ttttgcaatg cgatttctgc
     4261 tttcgtccct aaatggcgag ccagattaac cgtggcaaac agcaggtccc ccatttcctc
     4321 ctccagttta gcctggtcga caacagcctg ccgcgcctcg tacatcacct cgtcgatctc
     4381 ttcgtagact ttatcaacca ccgggccaag cgtcgtccag tcgaagccaa cgttggcgca
     4441 acgtttctgg attttttgcg cccgcattaa agccggtaaa ctacgaggaa tatcgtccag
     4501 cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg atttgctccc aacgggcaag
     4561 cacttcacta ctgttttcgg cagaactatc agcaaaaaca tgcggatggc gacgctctaa
     4621 tttatcgcta atagcagcgc aaatatcatt aaagtcaaag cgcccttctt cctgagccat
     4681 ttgcgcgtaa aacaccacct ggaatagcag atcgcccagt tcaccgcgaa gatcgtcaaa
     4741 atcttcacgg gcgatggcgt ccagcacttc gtaggtttct tcaagggtgt aaggcgcaat
     4801 ggtggcaaat gtctgctctt tatcccacgg gcagccgttt tccggatcgc gcaggcgctg
     4861 cataatagtg agcaaacggt cgatttgatt cattgaattg tcctgaaaat tgcgggtctg
     4921 tcaggtgtaa taccttcgag taaaagatat cgctggatac ggctgacgcc atatccagct
     4981 atgtaagact aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     5041 ttacccagca cgcgccccag cacttgcagg ttgtaaatct caatggtcat gtcgatggtc
     5101 gccagttgct gtttggtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     5161 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5221 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5281 gcatgggagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgt
     5341 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5401 tggtgcatca ggttaccaac accttcaacc accacacgac cgttatcttt actgcggttt
     5461 tgcggtgtgt agcttttttg ctgaagctgt ttcagcgcgg cggcgtcctg ctcttcggca
     5521 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5581 ccaccaatcg ccgccagcaa ctcgtcgaca tcattgaagt tgtaacgcgg cagcagatgt
     5641 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5701 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc
     5761 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5821 tgtttctggg tgataatttc aatctggtcg cccatttgca gctggtaggt gaacggcaca
     5881 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5941 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     6001 acatacaccc ggtcgtcaaa cacctgacta cgtacttcgt cgagcatttc gccggaatca
     6061 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     6121 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6181 tccgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt tccacccggc
     6241 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacgtaatcg
     6301 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcataacaa
     6361 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcgaaggcg
     6421 aggttctttt tctgcatttt gcgccagatg ctgtagatgt gtttcggacg accatacact
     6481 tcagctttaa cgccttcagc tttcatctca gcgcgcagat ggccgacaaa ctcttcgata
     6541 tagtgttcgc ggtcgagacg gcgttcatgc agcagttttg cgatgcgttt gtattcggtc
     6601 gggtggagat aacggaagca gtaatcttcc agctcccatt tcagttgccc aatcccaaga
     6661 cggttcgcca acggcgcgta gatattggtg cactcttttg ccgccagtac acgttcatct
     6721 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6781 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6841 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6901 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6961 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     7021 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     7081 ccctgcgtct gttgcagaca atacgcccag gtttcggcta agcactcaca cgacttctgg
     7141 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7201 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7261 caaatcgtaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7321 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7381 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7441 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7501 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7561 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgcag
     7621 gaacaccttc tacaccgacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7681 ccataccgca gaacagatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7741 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7801 aagttaagcg caacccgttt gagtcatacc agggcatctc accagagacg gtttcgagta
     7861 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7921 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7981 ttgcctgtac cagttcaaca tgaccaagat ggcgcatagc ttgtaagctg cccagacatg
     8041 ccctgacttt gggcagcaat gcttcaagtt ggggcgctaa aatggggcat tgtttgacgt
     8101 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     8161 agtagttcag acttaaacgc gcgcggcggc gatagcccca gggaacatcg gcgatcactt
     8221 cagagacatc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8281 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8341 tttcgcgttc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8401 tatcttcagt aacagcaact tccgcgtttt cctgcggcaa taatccgggg ataaataacg
     8461 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8521 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8581 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8641 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8701 ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8761 ggtgctagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8821 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8881 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8941 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     9001 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     9061 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gcttgagctg
     9121 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttcctg cgtgatgatg
     9181 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9241 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9301 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9361 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9421 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttggatct ggcgaaaaag
     9481 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9541 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9601 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9661 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9721 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9781 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9841 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9901 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9961 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
    10021 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccgggctg
    10081 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
    10141 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10201 attatcgaag ggccatccat ccagtgcctc gcaggtaagc gcctggccta tgtcgaacca
    10261 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10321 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10381 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10441 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagttaatgc tgaaaaactc
    10501 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10561 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagt
    10621 aagctggcaa tgacagtcat ggcggttgat gacaaccccg ctaacctgaa acttatcggc
    10681 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcagtt
    10741 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gccggacatg
    10801 gatggcattc gggcctgcga actcatccac caactcccgc atcagcgaca aacgccggtt
    10861 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10921 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10981 aagcctggca gcggtatttc gtctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    11041 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    11101 ttagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    11161 gaggaacagc tggctggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11221 ggcagttgcg gctatagcgg cgtaccacgt atgaagaatc tctgccagct gatcgaacaa
    11281 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11341 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11401 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11461 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11521 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11581 accggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11641 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11701 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11761 acccagcccc ggtttttctg gcacctgtac cagcccgcct ttgatctcaa acggttcttt
    11821 ggtcaggcgc tgattacctt cctgccagat ccagtgcgta tcaatagcag taattttacc
    11881 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11941 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    12001 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    12061 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    12121 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccac acggatcttc
    12181 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12241 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12301 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12361 atatttttca tacgccgctt ccgccaggcg caccaccgca tccggcgtca tcgcttcttc
    12421 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12481 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12541 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12601 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12661 gcgcccacta gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12721 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12781 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12841 attacgcgtg aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatgacc
    12901 cgccaccggg atgacctgca tttcagtaac aacaggcgtc gtaaattgag aactcatatt
    12961 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    13021 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgcaagg cagacgttta
    13081 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    13141 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13201 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13261 acatgggtaa atatcgccag agagatatcg aaatggttgt tggaatggca gccccaggtc
    13321 agcccccagt cgtcgcaaag ctgcgccacg cggactgcac cggaaagcgt ccagaagtgc
    13381 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13441 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgtcgaaa ctccgccatc
    13501 acttcacgtc cggagaagcc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13561 tcattcagcc ctttgcataa agaaatggct tcatcaagca gccatgcacc gttgggatca
    13621 acggtaatcc gcgcatccgg gaagcttttc ttcaatgcac gaacagtgtc gatttcttgc
    13681 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13741 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13801 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcacct
    13861 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13921 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13981 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccaaaggt atcaaaatcg
    14041 gctgcctgat tacctttatg tacctgctga accactttat tcaggcgcgc aacttcctgg
    14101 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    14161 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca cgatattgcg ggtgaagtat
    14221 gcgttatgcg caccaccaat attgagcaac atgctgtcat gtccggcgac cggaatgact
    14281 ttcatatcag taataacagg actggattgt gtcgccatct tattgccccg cgacaggttt
    14341 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattagggc
    14401 atgaacacca acataaatca gcgccccatt aaacgagcca gtcgtgccaa cgatataacc
    14461 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14521 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14581 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14641 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14701 aatgttcagc gatcccgtgc ggcgcatcag ccagtcggaa ataattccac ccagcacacc
    14761 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14821 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14881 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gctgtttgat
    14941 ctgcccccac ttcacgctga acggcacttt aactttggtg ctttgctgat ccatattgat
    15001 cagcgcgcca cccgcggcga tatactccag ctctttctga tttacccccg gatgttgatt
    15061 tggctcgtgg atgactttca accagataaa gctgatgaca atgcccagac cgcccataaa
    15121 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15181 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcac
    15241 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15301 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15361 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15421 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15481 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15541 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15601 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15661 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15721 acatcgccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15781 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15841 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15901 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gtaaaaacga
    15961 agaaataaag aagagtatga aatggatcgc ttgactctag gcaaacgcca gtaaaaatcc
    16021 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    16081 acgacattta ccgctcgctg acatatcagg acaacagcgt gccccactgt tcgacccacg
    16141 gatttgattc cgtttccggt tccgggttct cactggcgtc aatcaacagc atttcgccaa
    16201 cccgctgagc gctctgttcc tgcaacaagg catcgaactg tttgccgcca ttgcaaaaat
    16261 ttacataact gctgtcgccg agcgcaatca cgccataacg cagattcggc tggaagccca
    16321 gactatcttt gattccctga aagagcggca caatgctatc aggaaggtcg ccctgcccgg
    16381 tcgtggacgt aaccaccaga acatacttat cctgataggg cagccagtcg cttaattcag
    16441 gatcttcaaa taccgttgct ttgtggccct gtgcggtcag aattgcttct gcttcttccg
    16501 ccactaacag cgaatttccg tacatggtgc cgacaaaaat accaatttcc gccataccgt
    16561 tttctccctg gattaggaac ttatctctcc atcctgaccc gatggcgcac tgaactcaac
    16621 cctttcattt tcaggaagca gaccgcgcca gccaaattgt gataacgcct gcatccaggt
    16681 gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta aaaggatgcg tcaatgacaa
    16741 ctgactggca tgtagcatta accgctggag gccaaaatgc tcagcaccgc tgcgattctg
    16801 gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga cgcaaatggg caagatgtcg
    16861 tcgaagctga tgtttgcgtc cggttttcgg ctccagttcc accaggccgt agcgcgtggt
    16921 cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac
    16981 tgctggctgc gggcctttat cttcgcgggc aaatttatca gcgattttgt ccagttcttc
    17041 caccagtgga taatccaaca ccgcttcttc catcaaccag ccgcgcacaa tcgcatggta
    17101 acgtttctgg atttggtgct gttcaaactg ttgtgccagc agccgtccgg cctcgctgga
    17161 taatcccatc aacaacacac cagaagtggg tcggtccaga cgatgagcag taaaaacatg
    17221 ctgtcctatc tggtcacgca cggtttgcat gaccactact ttctcgtcgc gatccagcca
    17281 gctacggtga accagccagc cggagggttt atttaccgca accagccatt catcctgata
    17341 gagtatttcc agcattagct cgcatcatcc gcaaaaagag catccagttt ttccaactca
    17401 gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg ccatttcata atagggtgca
    17461 acagcaaaag cgcccggtaa cggctggttg ttatttaaca aatcgtgcat tcgcgggatc
    17521 agcccccact gcaaccactc cagtggttcc atggtgtcca taaagaacgg ttgggtacta
    17581 ttaaattgat gcggctgggg ttcatcgttt cgccagtgct gatgttcacg cagtaacgct
    17641 tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg tcataaagca acctcaataa
    17701 gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga gcactgtatt cacagtgctc
    17761 ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc ctgctcatcc ttgacaactt
    17821 ttcctctggc cttgcggcca atcgttcatc ctgaactatt gcttcctgct cacaccaccc
    17881 cgatgtgata cttcatcctg aagtgtccct ggccttcctg acccaccgaa tcatcctgac
    17941 cggttctcat tctccatcct ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct
    18001 tcatccagaa gcctttccct gaaacaccat cctggtgtgt cctgcagaag tgtcatcatc
    18061 ctgatgttca cttcgttgtc tgactccctg tcgacgaaga tagaatcgtc tttttcgcga
    18121 agtcttacaa ggcgcttaca gacaatgcct taaggaaaat ttcatatgaa agttaagaat
    18181 taacaatcaa cattttgatt ttaaatgaaa ataacgattc ttatcttttt aagtattcgc
    18241 ttatttcctg gcgatctcct acagagtctg taagagatct ctcacaacca gatggggtat
    18301 ttggattaca gaagaggctt aagttgatta aggaattccg caagatttga ggctaaattc
    18361 gtccgtttac gtgttcccag cgtctctttg cacacttcac cggagagatt acacaccgag
    18421 atgacctcta actcttcttc cagcgtcgcg ataaatagcg tgggcggcag ttttagcctt
    18481 ttctgagtca ccagatgacc aatcagattt tcctgcaccc gacggaagtc gtcttcactc
    18541 caggtttgca gcaatgtcag tttgatatcg ccaaactgcg cgtgcatatc cccggcaaac
    18601 tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc
    18661 gcgtttacat tttgttcccc ggtaaacggc tggggttgcc agtatacggc atcttcggta
    18721 gttgaaataa tgcacggtga aggaacaccg tacaattcct cacttaacgg ccagctttta
    18781 tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc
    18841 aaatcgtcca ctaatttctc tcttcactta aaccagatac acttgtcgtt tagtttatct
    18901 ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc aggcacttgc gggcctgact
    18961 cttggaaaat caaccgatta ccgggatacc tatgacgcca gcctactgca aggcgttcca
    19021 cgcagcctga atcgcgaccc gctgggtctg aaagcggata acctgccttt tcacggtacg
    19081 gatatctgga cgctgtatga actttcctgg ctgaatgcga aaggtttgcc gcaggtcgct
    19141 gtcggtcatg ttgaacttga ttacaccagc gtaaatctga ttgagtcgaa gagttttaag
    19201 ctctatctca acagttttaa ccagacgcgt tttaataact gggatgaggt gcgccagacg
    19261 ctggagcgcg acttaagcac ttgcgctcag ggtaagatta gcgtggcgtt atatcgtctt
    19321 gatgaactgg aaggccagcc gataggtcat ttcaatggca cttgcattga tgaccaggat
    19381 atcactatcg ataactatga attcactact gactatctgg agaatgccac cagcggtgaa
    19441 aaagtagtgg aagagacgct cgtcagccac ctgctgaaat caaactgcct gatcacccat
    19501 caaccagatt ggggttcgat ccaaattcag tatcgtggac gccaaattga cagagaaaaa
    19561 ctgctgcgtt acctggtctc attccgtcat cacaacgagt tccacgaaca gtgcgtggaa
    19621 cgcatcttta atgacctgtt acgcttctgc cagccagaga aattgagcgt ttacgcacgt
    19681 tatacccgtc gtggcggtct ggacattaac ccgtggcgca gtaatagcga ttttgtccca
    19741 tcgaccacaa gactggttcg gcaataaatt ttttctcaat tttgcgtgct ggattcacgc
    19801 agaaggttgt gaaaggtcat caggcagggc tattgtaatc aaagggaatg acgatattcg
    19861 tcccataagg agtttttctt gattacacat attagcccgc ttggctccat ggatatgttg
    19921 tcgcagctgg aagtggatat gcttaaacgc accgccagca gcgacctcta tcaactgttt
    19981 cgcaactgtt cacttgccgt actgaactcc ggtagtttga ccgataacag caaagaattg
    20041 ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc gtgaacgcgg cgtaaagctg
    20101 gaactgatta atcccccgga agaggctttt gtcgatgggc gaattattcg cgctttgcag
    20161 gccaacttgt tcgcggttct gcgagacatt ctcttcgttt acgggcaaat ccatcatacc
    20221 gttcgttttc ccaacctgaa tctcgacaac tccgtccaca tcactaacct ggtcttttcc
    20281 atcttgcgta acgctcgcgc gctgcatgtg ggtgaagcgc caaatatggt ggtctgctgg
    20341 ggcggtcact caattaacga aaacgagtat ttgtatgccc gtcgcgtcgg gaaccagctg
    20401 ggcctgcgtg agctgaatat ctgcaccggc tgtggtccag gagcgatgga agcgccgatg
    20461 aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag acagtcgttt tattggtatg
    20521 acagagccgt cgattatcgc cgctgaaccg cctaacccac tggtcaacga attgatcatc
    20581 atgccggata tcgaaaaacg tctggaagcg tttgtccgta tcgctcacgg catcattatc
    20641 ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt tgctgggaat tttaatgaac
    20701 ccggccaaca aagatcaggt tttaccattg atcctcaccg gcccgaaaga gagcgccgac
    20761 tacttccgcg tactggacga gtttgtcgta catacgctgg gcgaaaacgc gcgccgccat
    20821 taccgcatca tcattgatga cgccgctgaa gtcgctcgtc agatgaaaaa atcgatgccg
    20881 ctggtgaaag aaaatcgccg tgatacaggc gatgcctaca gctttaactg gtcaatgcgc
    20941 attgcgccag atttgcaaat gccgtttgag ccgtctcacg agaatatggc taatctgaag
    21001 ctttacccgg atcaacctgt tgaagtgctg gctgccgacc tgcgccgtgc gttctccggt
    21061 attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca ttgaagagtt tggtccttac
    21121 aaaatcaacg gcgataaaga gattatgcgt cgtatggacg acctgctaca gggttttgtt
    21181 gcccagcatc gtatgaagtt gccaggctca gcctacatcc cttgctacga aatctgcacg
    21241 taatctcccc tcc
//
LOCUS       DAJTSF010000069        20777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000069 DAJTSF010000000
VERSION     DAJTSF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.069"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..579
                     /locus_tag="OO335_002678"
     CDS             <1..579
                     /locus_tag="OO335_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6003015.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="OO335_002679"
     CDS             597..1235
                     /locus_tag="OO335_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6003016.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="OO335_002680"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="OO335_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6003017.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO335_002681"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO335_002681"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6003018.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="OO335_002682"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="OO335_002682"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6003019.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO335_002683"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO335_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6003020.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="OO335_002684"
     CDS             complement(5552..6613)
                     /locus_tag="OO335_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6003021.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="OO335_002685"
     CDS             complement(6610..8007)
                     /locus_tag="OO335_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6003022.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="OO335_002686"
     CDS             8163..9161
                     /locus_tag="OO335_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6003023.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO335_002687"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO335_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="OO335_002688"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="OO335_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO335_002689"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO335_002689"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR6003024.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO335_002690"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO335_002690"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR6003025.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..14855)
                     /locus_tag="OO335_002691"
     CDS             complement(14232..14855)
                     /locus_tag="OO335_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6003026.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14852..15274)
                     /locus_tag="OO335_002692"
     CDS             complement(14852..15274)
                     /locus_tag="OO335_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6003027.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15296..16071)
                     /locus_tag="OO335_002693"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="OO335_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16089..17141)
                     /locus_tag="OO335_002694"
     CDS             complement(16089..17141)
                     /locus_tag="OO335_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR6003028.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="OO335_002695"
     CDS             complement(17172..18014)
                     /locus_tag="OO335_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR6003029.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="OO335_002696"
     CDS             complement(18001..18882)
                     /locus_tag="OO335_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR6003030.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="OO335_002697"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="OO335_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20200..>20777)
                     /gene="ycjM"
                     /locus_tag="OO335_002698"
     CDS             complement(20200..>20777)
                     /gene="ycjM"
                     /locus_tag="OO335_002698"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR6003031.1"
                     /translation="NWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAIL
                     LSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAVY
                     HELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHVN
                     ITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5071 a         5667 c         5052 g         4987 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccac
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagttta
//
LOCUS       DAJTSF010000070        20712 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000070 DAJTSF010000000
VERSION     DAJTSF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.070"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO335_002699"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO335_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6003032.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO335_002700"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO335_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6003033.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO335_002701"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO335_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6003034.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO335_002702"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO335_002702"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6003035.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="OO335_002703"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="OO335_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6003036.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="OO335_002704"
     CDS             complement(8217..9110)
                     /locus_tag="OO335_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6003037.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO335_002705"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO335_002705"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6003038.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO335_002706"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO335_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6003039.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="OO335_002707"
     CDS             complement(10241..11473)
                     /locus_tag="OO335_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6003040.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO335_002708"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO335_002708"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6003041.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO335_002709"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO335_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6003042.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="OO335_002710"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="OO335_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OO335_002711"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OO335_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6003043.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OO335_002712"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OO335_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6003044.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OO335_002713"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OO335_002713"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6003045.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="OO335_002714"
     CDS             complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="OO335_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR6003046.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4980 a         5727 c         5032 g         4973 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatcctca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgcc cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgggcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
    20641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
    20701 ccgacaatgt gc
//
LOCUS       DAJTSF010000071        20692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000071 DAJTSF010000000
VERSION     DAJTSF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.071"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            82..351
                     /locus_tag="OO335_002715"
     CDS             82..351
                     /locus_tag="OO335_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6003047.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            345..710
                     /locus_tag="OO335_002716"
     CDS             345..710
                     /locus_tag="OO335_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6003048.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OO335_002717"
     CDS             complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OO335_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6003049.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1537..2220
                     /gene="gntX"
                     /locus_tag="OO335_002718"
     CDS             1537..2220
                     /gene="gntX"
                     /locus_tag="OO335_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6003050.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2279..2854
                     /gene="nfuA"
                     /locus_tag="OO335_002719"
     CDS             2279..2854
                     /gene="nfuA"
                     /locus_tag="OO335_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6003051.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3215..4531
                     /gene="gntT"
                     /locus_tag="OO335_002720"
     CDS             3215..4531
                     /gene="gntT"
                     /locus_tag="OO335_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6003052.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OO335_002721"
     CDS             complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OO335_002721"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6003053.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OO335_002722"
     CDS             complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OO335_002722"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6003054.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9675..12380
                     /gene="malT"
                     /locus_tag="OO335_002723"
                     /pseudo
     CDS             9675..12380
                     /gene="malT"
                     /locus_tag="OO335_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OO335_002724"
     CDS             complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OO335_002724"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR6003055.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OO335_002725"
     CDS             complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OO335_002725"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR6003056.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14850..16448
                     /gene="rtcR"
                     /locus_tag="OO335_002726"
     CDS             14850..16448
                     /gene="rtcR"
                     /locus_tag="OO335_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR6003057.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16430..17188)
                     /locus_tag="OO335_002727"
     CDS             complement(16430..17188)
                     /locus_tag="OO335_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6003058.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17205..18035)
                     /gene="glpG"
                     /locus_tag="OO335_002728"
     CDS             complement(17205..18035)
                     /gene="glpG"
                     /locus_tag="OO335_002728"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6003059.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18080..18406)
                     /gene="glpE"
                     /locus_tag="OO335_002729"
     CDS             complement(18080..18406)
                     /gene="glpE"
                     /locus_tag="OO335_002729"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6003060.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18596..20101
                     /gene="glpD"
                     /locus_tag="OO335_002730"
     CDS             18596..20101
                     /gene="glpD"
                     /locus_tag="OO335_002730"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6003061.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20370..20600)
                     /locus_tag="OO335_002731"
                     /pseudo
     CDS             complement(20370..20600)
                     /locus_tag="OO335_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4890 a         5693 c         5217 g         4892 t
ORIGIN      
        1 agtcagtaga tactctaccc tgcttaagtg ctactttgtg ctatcttgca tgtaacacaa
       61 agtagcactt agggaactga tatgcagaca gtgaaattac ggcaacaagg aggagccatg
      121 atcgttacta tcccacgaga tcttgccatc gatcttggct ggtcaccggg cacagagctt
      181 accgtggaga aaaaaggcga tagcgttaat ttgcgcgcca cagagcacaa accgcgtggg
      241 cgtcttaccg tcgcgcagtt gttgagccaa atcgatgaaa gcgaaattac ggagctaaat
      301 cagtccacag aaggatgggc tgaagggaaa aaaggtaatg aagcatggta aaggcacgga
      361 cgccacatcg tggtgagatc tggtatttta accctgatcc ggttgccggg catgaacttc
      421 aggggccaca ttattgcatt gtggtaacgg acaaaaaact caacaatgtt ttaaaagttg
      481 ctatgtgctg cccgatttca acaggggcaa atgcagcacg ttccacaggg gtgacggtga
      541 acgtcctccc ccgtgatacg caaaccggta acctgcatgg cgttgtactt tgtcaccagc
      601 taaaagccgt cgatcttatt gcccgtggcg ctaaatttca taccgttgcc gatgaaaaat
      661 tgattagtga agttatcagt aaactggtga atttaatcga cccacaataa ttagctctca
      721 cttaccacca acaccctctg cttcaacgcc accagcaggt gacaaaactc gaccggatgc
      781 gaaataaacg gcgcatgggc cgctttggcg aagatatatg attcgctgtg aggccaaagt
      841 ttatccagca tcggcaccac tttgcgcggc accagaccgt cgagatagcc atacaatcgc
      901 aaaaacggca tggacacgtt ttgcagcggc tgacggagat cgaccgtttt caggatttcc
      961 agcccgccat taagcacgtc aacctccggc atcggtaacg ccagaacggt tttcttcaac
     1021 gcccgcgcat cctggcgcgc cgtttcagtc cccatggttt gtaacgccag gaaccgctcc
     1081 actgtacgct gaaaatcatc actgagttgc tgctgaaatc ccgccagcac gtccggtttt
     1141 atccccggcc actcgtcacg agcactaaaa caaggtgacg acgccacggt gaccagcgcc
     1201 tgaacacgct cgggatgggt taacgcaatc tggcttgcca ccagcccgcc cagactccag
     1261 cctaaccaaa tggctttatc aggtgcctgt tgcagcacgg cttcggccat atcagcaagt
     1321 gacagcgcac caaatccccg gctacgcccg aagccgggca ggtcaacaag gtgcagcgta
     1381 aaatgcgagc taagttcctc gtcaatgcaa cgccacactt cggcattcag tccccatccg
     1441 tgcagcagca caagatgaac attcccctga cctttggtct gccaccagat gttattcatc
     1501 cgctattgtt ctcttttgac ttacaaggat gaacatatgc taacagtacc gggattatgc
     1561 tggctatgcc gaatgccact ggcgttaggt cattggggga tttgttcggt ctgttcacgc
     1621 gccacccgca cagataaaac gttatgccca caatgtggat tacccgccac acactcccat
     1681 cttccctgcg gtcgctgcct gcaaaaaccg ccgccctggc aaagactggt cacggttgcc
     1741 gactatgcgc cgccgttaag tccgcttatc caccagctta aattttcccg gcgcagtgaa
     1801 atcgccagcg ccctgtcacg tctgttacta ttggaagtct tacacgctcg tcgcaccacc
     1861 gggttgcaat tgccggatcg catcatcagc gttccgttat ggcagcggcg tcactggcgt
     1921 cggggattta atcagagcga tttgctgtgt cagccgttat cacgctggtt gcactgccaa
     1981 tgggatagcg aagccgtcac acgtacacgg gccactgcga cccagcattt tctcagtgcc
     2041 cggctgcgca agcgcaacct gaaaaatgcc tttcgtcttg aattgcccgt gcaaggtcgc
     2101 catatggtga ttgtggatga tgtcgttacc accggaagta ccgtcgcaga gattgcgcag
     2161 ttgcttttac gcaatggtgc ggcgactgtc caggtctggt gcctttgtcg aaccttgtag
     2221 agcctcgatg atgggcgtat tataaccaac taaaatagtc aactattagg ccattactat
     2281 gatccgtatt tccgatgctg cacaagcgca ctttgccaaa ctgctggcaa atcaggaaga
     2341 agggacacaa atccgcgtat ttgtgattaa ccctggcacg cctaacgctg aatgtggtgt
     2401 ttcttattgt ccgccggatg ccgtggaagc caccgacact gccctgaaat ttgacctgct
     2461 gaccgcgtat gttgatgagt taagcgcacc atacctggaa gatgcagaga tcgactttgt
     2521 taccgaccag ttgggttctc agttaacgct gaaagccccg aacgccaaaa tgcgtaaagt
     2581 ggcagacgat gcaccgctga tggagcgcgt ggagtatatg ctgcagtcgc agatcaaccc
     2641 acagcttgct ggtcacggtg gtcgcgtttc gctgatggaa atcaccgaag acggttacgc
     2701 cattctgcaa tttggcggcg gctgtaacgg ttgttccatg gtcgatgtga cgctgaaaga
     2761 agggatcgag aagcagctgc tgaacgaatt cccggagctg aaaggtgtgc gcgatctcac
     2821 cgaacaccag cgcggcgaac actcctacta ctaagttatc ccctcttttg tagattgccc
     2881 gatgcgacgc taaagcgtca tttcgggcct acgttgaaca tgtgccagac gttggcacag
     2941 cattgctttg gcaaatccct cccttcgttt ccccgcgata atatgaccaa cctctcataa
     3001 tttaaattta ccccgctctg gtgattctca aacgccaaat gttacccgta tcattcacat
     3061 gggtaccaaa catactcctg acatctgact acaataatta gttttagtgg gtatcagtcg
     3121 tggtgccgca atatctctgt tcccgattgg gataattaga gtttgtcgtc agaaaattga
     3181 cgttacccat aacaaatgaa aggccaggta aatcatgcca ttagtcattg ttgctatcgg
     3241 tgtaatcttg ttgttgctcc tgatgatccg cttcaaaatg aacggcttca tcgctctcgt
     3301 cctcgtggcg cttgctgttg gattaatgca aggaatgccg ctggataaag ttattggctc
     3361 catcaaagcc ggtgtcggcg ggacgctcgg tagccttgcc ctgatcatgg gttttggcgc
     3421 aatgctgggc aaaatgctgg cagactgcgg tggcgcacaa cgtatcgcca ccacgctgat
     3481 tgccaaattt ggtaaaaagc acatccagtg ggcggtggtg ttaaccggtt ttaccgttgg
     3541 ttttgccctg ttctatgaag tgggcttcgt gctgatgctg ccgctggtgt ttaccatcgc
     3601 ggcttccgcg aatattccat tgctgtatgt tggtgtacca atggcggctg cactgtctgt
     3661 gacccacggc ttcctgccac cgcatccggg cccgactgcg attgccacca ttttcaatgc
     3721 cgatatgggt aaaaccctgc tgtacggtac tattctggca atcccgaccg tgattctcgc
     3781 cggtccggtt tacgctcgcg tgttgaaagg tatcgataag ccaatcccgg aaggtctcta
     3841 cagcgcgaaa accttcagcg aagaagagat gccgagcttt ggcgtcagcg tctggacctc
     3901 tctggtgccg gttgtgctga tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg
     3961 tcacgctttc ctgccggtag cggagttcct cggtgacccg gtaatggcaa cgctgattgc
     4021 cgtgctgatt gcgatgttca cctttggtct gaaccgtggt cgttcaatgg atcagattaa
     4081 cgacacgctg gtttcttcca tcaaaatcat tgcgatgatg ctgttgatca tcggtggtgg
     4141 cggtgcgttc aagcaggtgc tggtagacag cggcgtggac aaatacattg cttccatgat
     4201 gcacgaaacc aacatttctc cgctgctgat ggcctggtcg attgctgccg tactgcgtat
     4261 cgcgctgggt tccgcaaccg ttgcggcaat cactgcgggt ggtatcgcgg caccgctgat
     4321 tgcaacgacg ggtgttagcc cagagctgat ggttattgcg gtgggttccg gtagtgtgat
     4381 tttctctcat gtgaacgatc cgggcttctg gctgttcaaa gagtacttta acctgactat
     4441 cggcgagacc atcaaatcct ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt
     4501 aggctgtctg ctgctgaata tggtgatttg ataacactga ctgccggatg catcgcatcc
     4561 gacatcaacc gcactctact tcttcttcgc tgcagctctg cgccgtctgt ccaaatcctt
     4621 cagcaacttg ttcacgccat catcggcaaa catcgactca agcgttgcgg aaagcttgcg
     4681 tcgccagttt ttatactggt aactggtgcc aggaatattc accggttcgg ccatatccag
     4741 ccagtcttcc ggctgtagtc ctaacagagc actgttactg tcggcaatgt agcgctgcaa
     4801 accacggttc agcgtcggcg tcatcgacat caacgatgcc ttatgcccgg cacgttttgg
     4861 cagacaacca tatttatgca gtgcatccag cagcccttgc ttcgccagtt cgcgatcctg
     4921 atacagaccg cgcagtacca cttcatccgg atacagcccc agggttttgc ccagcgttag
     4981 atccccgctc tcccagtaac cacgcagcgt tggcaggtca tgtgtcgccg caaccgccat
     5041 cgactgctcc ggatacgctt tcggtgcacg gaacgtcttc tcgtggtcgt tttcgaaata
     5101 gagcactttg taagagtaca caccgctgct gcgcagctta ccaacaatct ctaccggtac
     5161 ggtaccgaga tcttcaccaa tcaccataca gcgatgacgt ttactttcga gtgccagaat
     5221 cgagagcaga tcatccaccg gatagtgaac atacgcgccc tgatctgccg tctcgccata
     5281 cggtatccac cacaaacgca gcatcgacat cacatggtca attcgtaatg cgccgcagtt
     5341 ttgcatattg gcacgcaaca gctcgataaa cggttcatag gcacgcgcgg tgatgatatg
     5401 cgggtccatt ggcggtaatc cccagttctg ccccaacggg ccgaggatat ccggcggcgc
     5461 gccaaccgat gctttcaggc aatatagttc acggtcacac caggtttccg ccccaccttc
     5521 cgctacgcca accgccagat cacgatacaa gccaatcggc atttcatagc cctggcttat
     5581 ctcccagcag gcggcaaact ggctgtaagc cagccactgc aaccagagat aaaaatcgac
     5641 gtcatcacga tgttcttcgc agaactgacg cacttctggt gaatccacgt tcttatacat
     5701 ctctggccat gcaggccagc cccagcgcat ttcgtcctct ttcacttgct gggcatgtag
     5761 cgcatcaaag gcagcctgcc agaacaggct gtcgccctgc tctgcaacaa actggcgaaa
     5821 cgcggccatc tgctcatcat cacgttgcgc gaaacctttc cacgccattc gtaatgctgt
     5881 catttttagg gcggtaaccg tggagtaatc gacccagtcg gcatcgcgcg cctgttgcag
     5941 cgtctgttgc gtggtcggca actgccacca ggcctgagcc tcttcgctaa gatggaaatc
     6001 ttcaacggcg ttaacgtcga tataaatcac attcagccaa cggcgagaag acgggctgta
     6061 tgggctggcg ctctccggat ttgccggata gagcgcatga atcgggttca ggccaatgaa
     6121 cgacccgcca cgttttgcca catccaccag catcgctttg agatcgccaa aatccccaat
     6181 accccagttt ttttccgatc gcagcgtata aagctgaacg caggcacccc acagcttttg
     6241 tttattcagc aaagcctgcg gttcgtaaca acgtttcggg gcgacaatca cccggcaatg
     6301 cgcgcgctgg tcgtcctggg tgagtgttag cgtgtgataa ccttccggca gcttcgtcgg
     6361 tagattgaac gctttgcccc ccgttacatg gcctttgtac tgcgttcctt cttcggtggt
     6421 cagctgccag ctatattcgc cgctgccctc caccaccatc ggcatttttt tgccgctggt
     6481 ataaaccatg acattcggga ctggcgttac cgccactttc gtggcggtac gttgatgcat
     6541 cgcgtcaagc aaacgccgtt tggtttcggc gctaatcgac tgcggtttac cgtgggcatt
     6601 gatgtaattg gggctaatcc ccgccgccag cgcggcatta tccagacgtt tgctttccat
     6661 cgagcttcct tagcgttttg cctgccagat acgagcctga taatcgcgaa tagagcgatc
     6721 cgagctaaac ataccgcagc gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg
     6781 gtcgcggtac agcacatcca cctgcttttg tgcctcaaca taggctgcga aatccgccat
     6841 caccagatac ggatcgccgc cctgtttgcc gatactgtgc agcatctggt cgaaggcatg
     6901 cttatcgccg tcgctgtatt taccgctttc cagctctttc aatactgcgt ccagcacctt
     6961 atctttcttc cgccatttca ccgggtcgta gcctttggcc agaatagcct tcacttgttc
     7021 cacggtatga ccaaaaataa agatattttc ttcaccgact ttctcggcga tttcaacgtt
     7081 cgcaccatcc agcgtaccga cagtaagcgc accattgagc gccagtttca tattgccggt
     7141 accggaagct tctttacctg cagtcgaaat ttgttcggag atatccgccg ccgggatcag
     7201 tttttccgcc gccgaaacgc aataatccgg caggaacacc accttcaact tatcgccaac
     7261 cagtggatcg ttgttgatca cgtcagccac tttgttgatc gcaaagataa tattcttcgc
     7321 caggtagtag cccggtgccg ctttcgcgcc gaagaggaag acgcgcggta cgcgatcagc
     7381 ctgcaggttt tcacgaattt ctttgtacaa cgccagaata tgcagcagat tcaggtgctg
     7441 gcgtttgtac tcatgcagac gtttgatctg aatatcgaaa atcgcctgtg gattgatctc
     7501 aataccggta cgaactttca caaactccgc cagacagact ttattcgcct gcttgatctc
     7561 gcgatattgc tgacggaatt tcgcatcatc agcgaatttt tccagattga tcagctgatc
     7621 gagatcgtta gcccactctt tttgcagtga tttatccaac agagccgcca gtgcagggtt
     7681 gcactgtttg atccagcgac gtggggtaat accgttggta acgttatgga atttgttcgg
     7741 ccatagctgg tgatattccg ggaacagatc tttcaccacc agatccgagt gcagcgccgc
     7801 aacaccgttc accgcgaaac cgccaaccac acacaggttc gccatatgca cttgtttatc
     7861 gtgcaccacc gccagtttgg cccacacttt ttcatcgccc ggccaggttt tctccaccag
     7921 cgttttaaag cgagtattaa tttcgttaat aatctgcata tggcgcggca gtaagccttt
     7981 caccagtttc acatcccagc gttccagcgc ttctggcatc agggtatggt tggtgtaagc
     8041 gaaagttttg ctggtgatgg cccaggcgtc atcccagctc atctggtgct catcggtcag
     8101 cacgcgcagc agttctggaa tcgcgatagt tggatgggta tcgttcagct gaataacttc
     8161 gtaatccgcc agttcgtgca gtttacgccc cgccagatga tggcgacgca aaatatccgc
     8221 taccgaacag gcacactgga agtattgctg catcaggcgc agctttttac cggcagtatg
     8281 gttgtcgttt ggatagagaa ctttggtcag tttttccgca ttgatgcctt gctgttcggc
     8341 gcgcaggaaa tcaccgtcgt taaatttagt tagatcaaac ggatgcgcat gcgtcgcctg
     8401 ccacagacgc agcggctgcg ccacgccatt acgatagccg acaacgggga gatcccacgc
     8461 ttgaccggta atggtaaact ccggctccca gcgtccgtct ttcatcactt taccgccaat
     8521 ccctacctgc acatccagtg cttcgttgtg gcggaaccac gggtagttac tgcgatgcca
     8581 gtcatccggc gcttcaacct gtttgccatc gacaaaagac tggcggaaca aaccatattg
     8641 atagttcaga ccgtaacccg tcgcagactg accgacagtt gccattgagt cgaggaagca
     8701 cgccgccaga cgtcccagac caccgttacc cagcgccggg tcgatctctt cttccagcag
     8761 gtcagtcagg ttgatgtcat aagccttcaa cgaatcctgt acatcctgat accagccgag
     8821 attcaacagg ttgttgcccg tcagacgacc aatcaaaaac tccattgaga tgtagttaac
     8881 atgtcgctga ttcgccaccg gtttggcgaa tggctgagca cgcagcattt cggccagtgc
     8941 ttcactcact gccagccacc actggcgagg agtcatttca gccgcagaat ttaagccata
     9001 acgctgccac tgacgtgaaa gcacttcctg aaattgctta tcgttaaaaa taggttgtga
     9061 cataggagtt ccacttttct tagattttca acacaacgtt atcgctagtt tgccaggctc
     9121 gatgttgacc ttcctcatcc tgcgggggat taggcaggga ggagttgcgg ggatgagcaa
     9181 ggaaatgtga tctcgaccac ttaaagctag tgcaaaccac aggattagca gcaaatcaat
     9241 gccatacagc gcagaaaatc tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa
     9301 aagcggaagg taactctata aattaagtaa aggagtgaaa cagtttcaca agtaaaatat
     9361 ccagtgtgct ccatctcatt cttaatagat ttattaagat catcttttta gatggcactt
     9421 tcatcaggaa tgaagaagaa acccttgctt aaatgaatct gatgaacata agggaaacca
     9481 gtattcacgc tggatcagcg tcgttttagg tgagttgtta ataaagattt ggaattgtga
     9541 cacagtgcaa attcagacac ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc
     9601 cgaccttata accattaatt acgaagcgca aaaaaaataa tatttcctca ttttccacag
     9661 tgaagtgatt aactatgctg attccgtcaa aactaagtcg tccggttcga ctcgaccata
     9721 ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc gaacaacttc cggctggcgc
     9781 tgatcacgag tcctgcgggc tacggaaaga ccaccctcat ttcccagtgg gcggcaggca
     9841 aaaacgatat cggctggtac tcgctggatg aaggtgataa ccagcaagag cgtttcgcca
     9901 gctatctcat tgccgccgtg cagcaggcaa ccaacggtca ctgtgcgata tgtgagacga
     9961 tggagcaaaa acggcaatat gccagcctga cgtcactctt cgcccagctt ttcattgagc
    10021 tggcggaatg gcatagccca ctttatctgg tcatcgatga ctatcatctg atcactaatc
    10081 cagtgatcca cgagtcaatg cgcttcttta ttcgccatca accagaaaat ctcaccctgg
    10141 tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa tctgcgtgtt cgtgaccaac
    10201 tgctggaaat tggcagtcag caactggcat ttacccatca ggaagcgaag cagttttttg
    10261 attgccgtct gtcatcgccg attgaagccg cagaaagcag tcggatttgc gatgacgttt
    10321 ccggttgggc gacggcacta tagctaatcg ccctctccgc ccggcagaat acccactcag
    10381 cccataagtc ggcacgccgc ctggcgggaa tcaatgccag ccatctttcg gattatctgg
    10441 tcgatgaggt tttggataac gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca
    10501 ttttgcgctc aatgaacgat gccctcatca cccgtgtgac cggcgaagaa aacgggcaaa
    10561 tgcgtctcga agagattgag cgtcaggggc tgtttttaca gcggatggat gataccggcg
    10621 agtggttctg ctatcacccg ctgtttggca acttcctgcg ccagcgctgc cagtgggaac
    10681 tggcggcgga gctgccggaa atccaccgtg ccgccgcaga aagctggatg gcccagggat
    10741 ttcccagcga agcgattcat catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata
    10801 ttctgcttaa tcacgcctgg agtttgttca accatagcga actgtcgctg ctggaagagt
    10861 cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc gcagttagtg ttattgcagg
    10921 cgtggctaat gcaaagccaa catcgctatg gcgaagttaa caccctgcta gcccgtgctg
    10981 aacatgaaat caaggacatc agagaaggca ccatgcatgc agaatttaac gctctgcgcg
    11041 cccaggtggc gattaacgat ggtaatcagg atgaagcgga acggctggca aaactggcac
    11101 tggaagagct gccgccgggc tggttctata gccgcattgt ggcaacctcg gtgctgggtg
    11161 aagtgctgca ctgcaaaggc gaattgaccc gctcactggc gctagtgcag caaaccgaac
    11221 agatggcacg ccagcacgac gtctggcact acgccttatg gagtttaatt cagcaaagtg
    11281 aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga aacgcaggaa aaagcgttcc
    11341 agctgatcaa cgagcagcat ctggaacagc tgccaatgca tgagtttctg gtgcgcatcc
    11401 gagcacaact gttatgggcc tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg
    11461 ggattgaagt cttgtcgtct tatcagccac agcaacagct tcagtgcctg gcaatgttga
    11521 ttcaatgctc gctggcccgt ggtgatttag ataacgcccg tagccagttg aaccgtctgg
    11581 aaaacctgct ggggaatggc aaatatcaca gcgactggat ctctaacgcc aacaaagtcc
    11641 gggtgattta ctggcaaatg accggcgata aagccgccgc tgccaactgg ttgcgtcata
    11701 cggctaaacc ggagtttgcg aacaaccact tcctgcaagg tcaatggcgc aacattgccc
    11761 gcgcacaaat cttgctgggc gagtttgaac ctgcggaaat tgttctcgaa gaactcaatg
    11821 aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg taacctgttg ctgcttaatc
    11881 aactgtactg gcaggccgga cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa
    11941 aactggcgaa tcgcaccgga tttatcagtc attttgtcat cgaaggcgaa gcgatggcgc
    12001 aacaactgcg ccagctgatt caacttaata cgctgccgga actggaacag catcgcgcgc
    12061 agcgtattct gcgagaaatc aatcaacatc atcggcataa attcgcccat ttcgatgaga
    12121 atttcgttga acgtctgcta aatcatccgg aagtgccaga actaatccgc accagcccgt
    12181 tgacccaacg tgaatggcag gtcctggggc tgatctactc cggttacagc aatgaacaaa
    12241 ttgcgggcga actggaagtg gcggcaacca ccatcaagac gcatatccgc aatctgtacc
    12301 aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca cgcgcagaaa ctgctgaaga
    12361 tgatgggtta tggggtgtga gtttagccgg ataatgcatt ggatccggtt tacatcttta
    12421 catcattcaa tactcacccg cgttacgcca tctgtttcta tcaaaccaaa ccgcaccggc
    12481 aagaaacgct ccaccaccgc gatattggtc agcagatggc atgaaggatg ggcgaccgta
    12541 aattctcccg cgcccgccag cgccatcggc agtaccagtt ggtcggcgag atattccccc
    12601 accgccgctg ggcttgccag gtagcgtttc acctctttca ctaactgcgc ggctaccacc
    12661 tcggcactga cgcgcttttc accgacgacg aaaaagcgtt cagtgatatt ttcactttcg
    12721 acttccagcg agacggtatt acccggcccc tggtcgcgcg gcaggttatg aatattctgt
    12781 tcatgcaggg aaaaaatccc cgccagtgta gcgatttcac gctcagcaac atggcgcggc
    12841 acaccagcta atagaacttc tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc
    12901 aaggtgctaa acgatgccac gggcgagact tccgttgcca ccacaccgcc tccggcagga
    12961 taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc ctattttcgc cagcagcggc
    13021 tccagcaccc ggcggataaa atccgcaggc ggcgccgacg ggttatcggt gccgccgctc
    13081 acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc
    13141 agcgtacaac ttccggcgct accgatagca aagcggtaat cgccgccgcg cacggtgcca
    13201 ggccggaaga ccagacgctg cgatcccagc tccgcgcctt ccaccgttgc cctacaaatt
    13261 tccgcagccg cttttaccgc ggtcagatgc tggcgcagca gccccggttt cgcccgtccg
    13321 gctcgaatgc cagtgatggt aaatggctgg ccggttatca tcgacaggct cagcgccgag
    13381 cgcaggatct gcccgccgcc ttcgccctgt gcgccatcca gcgcaatcat ccttttcatc
    13441 atttatcctt ttacgcacac cacctgacgc agggtgtaga taacttccac cagatcgctt
    13501 tgtgccgcca tcaccgcatc aatatcttta tacgccatcg ggatttcgtc gatcacttcg
    13561 gcatctttac ggcattccac atgcgcggtg gcacgaattt ggtcttccac gctgaacagt
    13621 tttttcgctt tagttcggct cattacccgc ccggcaccgt ggctgcacga acagaacgac
    13681 tcttcatttc ccagcccacg gacgataaag ctttttgctc ccatcgaacc gggaataatt
    13741 ccatattgac cagcacgcgc agacaccgca cctttacgcg tcacgtagat ctcttcacca
    13801 aagagctgtt ctttttgcac atagttgtgg tgacagttga tctcttccat cgccagcgtt
    13861 tgtggctgtc tgaccgtttt ctgcgtaatg ctctgcaacg ccgttaccac attttccatc
    13921 atcgcatcgc ggttaaggct ggcgaaaagc tgcgcccagg ccacggctct taggtaatca
    13981 tcaaagtatt ccgtaccttc cataaagtac gccagatcac gcgacggcaa cgtctcaagt
    14041 tggtcctgca tctctttttg cgccagatcg ataaagtaag tcccgatggc gttaccaatt
    14101 ccgcgtgaac cggagtgcag cataatccac acctgctccg actcatcaag gcagatttca
    14161 ataaagtggt tacccgttcc cagcgttccc aggtgtttat agttgttagt gttaaggaaa
    14221 cgcggatatt tttgcgttaa ccactgataa ccggcctcca gctcagccca tttcgcatcg
    14281 acgttaaaag gcgggttttc ccatgcgcct ttatcacgtt tacaacgccc ggtagtacgc
    14341 ccgtgtggta cggccgtttc aatcgcttga cgcagctccg ccaggttttc aggcaggtct
    14401 gccgccgtta acgcggtacg cagcgcgttc attccacagc caatatccac gcccaccgcc
    14461 gccggaataa tcgccccttt ggtcgggatc acgctaccaa tggtggaacc tttacccagg
    14521 tgtacatcag gcattaccgc aatatgtttg aaaataaacg gcatcttcgc cgtattaata
    14581 agttgctgac gcgcatcggc ttctaccggc acgcctttgg tccacatttt taccggggca
    14641 ttttcagtgg tcagtaattc gtaattcatt ttgtttttct cttttcgttg tttgcggtcc
    14701 tgataattgc aaccgtcgtg ccagaaaatt aaataagcag attaatttat tgtttctaaa
    14761 aagattatat ctttatatcc gtaaacggag atttcccgca aagccgattt accgataatg
    14821 aaatatcgcc ttttataagg atatctaaga tgcgtaaaac agtggctttt ggctttgtcg
    14881 gtaccgtgct ggattatgcc ggacgcggca gtcagcgctg gtcaaaatgg cgtccgtcac
    14941 tctgtatata ccagcaagaa tcgttggtca tcgatcgact ggaattattg cacgatgccc
    15001 gctcgcgctc gctatttgaa acgcttaaac gcgatatcgc cagcgtttcg ccagaaacag
    15061 aagtggtggg cgttgagatt gaactgcata acccgtggga tttcgaagag gtttacgcct
    15121 gcctgcatga tttcgcccgt ggttacgagt ttcagccaga aaaagaagac tatttaattc
    15181 acatcaccac cggcacccac gtcgcgcaga tttgctggtt tctgctggcg gaagcgcgtt
    15241 atctacccgc ccggttgata caatcttcaa ctccgcgcaa aaaagaacag ccgcgcggcc
    15301 caggtgaagt gacgattatc gatctcgatt taagccgtta taacgccatc gccagccgct
    15361 ttgccgagga acggcagcaa acgctcgatt tcctcaaatc cggtattgcc acgcgtaacc
    15421 cccacttcaa ccgcatgatt gagcagatcg aaaaagtggc gattaaatcc cgcgcgccga
    15481 ttctgcttaa cggcccgacc ggcgcaggta agtcatttct ggcgcgacgc atcttcgagt
    15541 taaaacaggc acggcatcag tttagcggcg cgtttgtgga agtgaactgc gccaccctgc
    15601 gcggcgatac cgcgatgtcg acgctgtttg gtcatgtgaa aggcgcgttt accggggcgc
    15661 gggaatctcg tgaaggatta ttacgcagcg ccaacggcgg aatgttgttt cttgatgaga
    15721 ttggcgaact gggcgcagac gaacaggcaa tgctgctgaa agctattgaa gagaaaacct
    15781 tttacccgtt tggcagcgat cgccaggtga gcagtgattt tcagcttatc gccggaacgg
    15841 tgcgcgattt gcgccagctg gttgccgaag gcaaattccg cgaagatctg tacgcgcgga
    15901 tcaatctctg gaccttcacc ctgccgggtc tacgccagcg ccaggaagat attgaaccga
    15961 acctggatta tgaagtggag cgccacgcca cactcactgg tgacagcgtg cgttttaaca
    16021 ccgaagcgcg gcgtgcctgg ctggcctttg caacatctcc ccaggcaaca tggcgcggta
    16081 actttcgcga actttctgcc agcgtcacgc ggatggccac ctttgccact agcggacgca
    16141 tcactctgga cgtggtggaa gatgagataa accgtctgcg ctataactgg caggagagcc
    16201 gcccctccgc gcttacggcg ttgctgggcg cagaggcaga aaacatcgat ctcttcgacc
    16261 gtatgcaact ggaacacgtt atcgctatct gccgccaggc aaagtcgctt tctgctgccg
    16321 gacgacagct ttttgatgtt tcgcgccagg gcaaagccag cgtcaatgac gcggatcggc
    16381 tacgcaaata cctggcgcgt tttggtctga cgtgggaagc cgtgcaggat cagcacagct
    16441 ccagttgaat atggtggtcc gtcagcacct gcatcacgct tactggcggc ggggcgtcgg
    16501 tgtagacggc atcaaccatg ctgatgctcc ccatattgac catcgcatta cggccaaatt
    16561 ttgaatggtc aaccaccagc ataacgtggc gcgagttctc aataatggcg cgcttggtac
    16621 gaacttcgtg gtaatcgaac tccagcagcg agccgtcgct gtcgatgccg ctgatcccca
    16681 gaatgccgaa atcaaggcgg aactgggaga taaaatcgag cgtcgcttcg ccaatgatcc
    16741 cgccatcgcg gctacgtaat tcgccaccgg cgagaataat gcggaaatct tcttttacca
    16801 tcaacgtgtt agcaacgttg agattgttgg tgacaatgcg caaattgctg tgattgagca
    16861 gcgcgtgcgc caccgcttcc ggcgtggtgc cgatatcgat aaacagcgtc gagccattgg
    16921 ggatttgctc cgccactttg cgggcgatgc gctctttttc ttcggtctgg gtggctttac
    16981 gatcgtgcca cggcgtgtta accgaactgg aaggcagcgc cgcgccgcca tgatggcgca
    17041 ggatcaggtt ttgctctgcc agctcgttga ggtcgcggcg aatagtctgc gggctgacgg
    17101 agaagtgctc caccagttcc tcggtactga cataaccctg ctgtttaacc agttcgataa
    17161 taccgttgtg acgttgtgtt tgtttcattt ataaatccct ggaattattt tcgttttcgc
    17221 gcattgagcg aatcaacaaa agccatcgct aaacccacgg ctaatccggc gatgtgtgct
    17281 ccgttcgcca tcgacatccc aaacaaatca aaccatccgg cgacaatcca gatcagcgca
    17341 aagataatta acccacgttg caggtaaatg ccactttgcg gatcgcgttc gccacgtagc
    17401 cagacgtagc ccatcagcgc atacaccacg ccagaaagcc cgccaaacca cggcccgctg
    17461 aatttttgct gcatatagcc gcttaacagg gcgctgataa gcgtaatgac aattagctta
    17521 ccgctaccga ggcgtttttc caccgcgccg ccgagatacc accaccaaag caggttaaag
    17581 aggatatgca tcagcgagaa gtgcattaac gcgtgggtga agtaacgcca gaactcaaat
    17641 ttcagtgttg gatcgaatgg ccaggccagc cataacatca cttcctgatc gccgagaatt
    17701 tgcatggcaa taaacaccac cacgcaggcg atcatcatca cccaggtcac cggacctgcg
    17761 cgttcacgca aggcagcaaa gaaaggataa cggcgataat gcaggccact gccggtatgg
    17821 cctgccagcc agctggccgc cagataacgc ggatctgccg ggttttcgag aaaacgcgcc
    17881 agctccgccc gcacgcgctc ggcctgggac tcatccgcca gccagacatc gctttggtta
    17941 tgttgttgaa tcgtgaggat aacaccctgc gtcgccatgt aatcaacaaa cgcctgcgcc
    18001 acgcgggggt tagcaaaaga ggtaatcatc aacatcgttg ctgtcgctta ttccacacaa
    18061 aaggggacag tataaagcgt tacgcgccgt acgccacctc tgcgggaaac tgtcgctgcc
    18121 aggcttcaaa gccgccgtca atgctataga ccacatcgta gccctgttgc agcagatact
    18181 gcgccgcccc tttgctgcta ttgccgtgat aacacatcac catcaccgga gtgtcaaagt
    18241 cgttatcacg cataaacgcg cccagcgtgt cgttggttaa atggaaagcc tgcaccgcat
    18301 gccccatagc gaaactctgt ggatcgcgaa tatcgaccag caccgcctct ttttcctgca
    18361 acttctggtg cgcgtcggca acgttaatac attcgaactg atccatgcgt ctctctttct
    18421 ttacaaacaa gtgggcaaat ttaccgcaca gtttacgtcg aagcggcaga taaacgccat
    18481 aatgttatac atatcactct aaaatgtttt ttcaatgtta cccaaagcgc gattctttgc
    18541 taatatgttc gataacgaac atttatgagc tttaacgaaa gtgaatgagg gcagcatgga
    18601 aaccaaagat ctgattgtga taggtggcgg catcaatggt gctggtatcg cggcagacgc
    18661 cgctggacgc ggtttatccg tgctgatgct ggaggcgcag gatctcgctt gcgcgacctc
    18721 ttccgccagt tcaaaactca ttcacggtgg cctgcgctac cttgagcact atgaattccg
    18781 cctggtcagc gaggcgctgg ctgaacgtga agtgctgctg aaaatggctc cgcatatcgc
    18841 cttcccgatg cgttttcgcc tgccacatcg tccgcatctg cgcccggcgt ggatgattcg
    18901 cattggtctg tttatgtacg atcatctggg taaacgcacc agcctgccgg gatcaactgg
    18961 tttgcgtttt ggcgcaaatt cagtgttaaa accggaaatt aagcgcggat tcgaatattc
    19021 tgactgttgg gtagacgacg cccgtctggt actcgccaac gcccagatgg tggtgcgtaa
    19081 aggcggcgaa gtgcttactc ggactcgcgc cacctctgct cgccgcgaaa acggcctgtg
    19141 gattgtggaa gcggaagata tcgataccgg caaaaaatat agctggcaag cgcgcggcct
    19201 ggttaacgcc accggcccgt gggtgaaaca gttcttcgac gaggggatgc atctgccttc
    19261 gccttatggc attcgcctga tcaaaggcag ccatattgtg gtgccgcgcg tgcataccca
    19321 aaagcaggcc tacattctgc aaaacgaaga taaacgcatt gtgttcgtga tcccgtggat
    19381 ggacgagttt tccatcatcg gcaccaccga tgtggaatac aaaggtgatc cgaaagcagt
    19441 gaagattgaa gagagtgaaa tcaattacct gctgaaagtg tataacgcgc actttaaaaa
    19501 gcagctaagc cgtgacgata tcgtctggac ctactccggt gtgcgtccgc tgtgtgatga
    19561 tgagtccgac tcgccgcaag ccattacccg tgattacacc cttgatatcc atgatgaaaa
    19621 cggcaaagca ccgctgctgt cggtattcgg cggtaagctg accacctacc gaaaactggc
    19681 agaacatgcg ctggaaaaac tgacgccgta ttatcagggt attggcccgg cgtggacgaa
    19741 agagagcgtg ctgccgggtg gtgccattga aggcgaccgc gacgattacg ccgctcgcct
    19801 gcgccgccgt tacccgttcc tgactgaatc gctggcacgt cactacacgc gcacttacgg
    19861 cagcaacagc gagctgctgc tcggcaatgc gggaacggta agcgatctcg gggaagattt
    19921 cggtcatgag ttctacgaag cggaactgaa atacctggtg gatcacgaat gggtccgccg
    19981 caccgacgat gccctgtggc gtcgcacaaa acaaggcatg tggctgaatg cggatcaaca
    20041 atctcgtgtg agtcagtggc tggtggaata tacgcagcag aagttgtcgc tggcgtcgta
    20101 aattaacgta aggtgatcag gtcagatttc aatctggcct gagactgatg acaaacacaa
    20161 aactgcctga tgcgctacgc ttatcaggcc tacgtggttt atgcaatata ttgaatttgc
    20221 atggtcttgt aggccagata agacgttcac gtcgcatccg gcatgaacaa agcgcacttt
    20281 gtcaaaaatc taacctactt tttaattcag gaaattaccg caaagcccac gccatcatac
    20341 ccaacgtaac aaaactcagg cacgttcccc actcgccccg agaaaaatag cattaatgcg
    20401 cccagcgtca gcataaaaat attgagcaga aggtaaattg ttggcgtaaa gagtaaattg
    20461 caaacctcag gaatagcggc aagcccagcc cccagcatgg caataaccgc tgcaaaaaat
    20521 tgcgtcagta ttgggatgcc gaaaaaagtc attaccagcg aggtcaaaat ccattttcta
    20581 ttttgcatta ttctttccat tctttctgaa tggcgaaatt atactccccg agtccccttg
    20641 ccccttctgg acacttttcc gaaatgatgg cgaaaaaaac gggacccttt gg
//
LOCUS       DAJTSF010000072        20602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000072 DAJTSF010000000
VERSION     DAJTSF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.072"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            97..396
                     /locus_tag="OO335_002732"
     CDS             97..396
                     /locus_tag="OO335_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6003062.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            406..930
                     /gene="ygaP"
                     /locus_tag="OO335_002733"
     CDS             406..930
                     /gene="ygaP"
                     /locus_tag="OO335_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6003063.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(977..1381)
                     /gene="stpA"
                     /locus_tag="OO335_002734"
     CDS             complement(977..1381)
                     /gene="stpA"
                     /locus_tag="OO335_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6003064.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2049..2498
                     /gene="alaE"
                     /locus_tag="OO335_002735"
     CDS             2049..2498
                     /gene="alaE"
                     /locus_tag="OO335_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6003065.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2535..2879)
                     /locus_tag="OO335_002736"
     CDS             complement(2535..2879)
                     /locus_tag="OO335_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6003066.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3031..3261
                     /locus_tag="OO335_002737"
     CDS             3031..3261
                     /locus_tag="OO335_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003067.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3608..3853
                     /gene="nrdH"
                     /locus_tag="OO335_002738"
     CDS             3608..3853
                     /gene="nrdH"
                     /locus_tag="OO335_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6003068.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3850..4260
                     /gene="nrdI"
                     /locus_tag="OO335_002739"
     CDS             3850..4260
                     /gene="nrdI"
                     /locus_tag="OO335_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR6003069.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4233..6377
                     /gene="nrdE"
                     /locus_tag="OO335_002740"
     CDS             4233..6377
                     /gene="nrdE"
                     /locus_tag="OO335_002740"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6003070.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6387..7346
                     /gene="nrdF"
                     /locus_tag="OO335_002741"
     CDS             6387..7346
                     /gene="nrdF"
                     /locus_tag="OO335_002741"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6003071.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7700..8902
                     /gene="proV"
                     /locus_tag="OO335_002742"
     CDS             7700..8902
                     /gene="proV"
                     /locus_tag="OO335_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6003072.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8895..9959
                     /gene="proW"
                     /locus_tag="OO335_002743"
     CDS             8895..9959
                     /gene="proW"
                     /locus_tag="OO335_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6003073.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10017..11009
                     /gene="proX"
                     /locus_tag="OO335_002744"
     CDS             10017..11009
                     /gene="proX"
                     /locus_tag="OO335_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6003074.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11201..12380
                     /locus_tag="OO335_002745"
                     /pseudo
     CDS             11201..12380
                     /locus_tag="OO335_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12504..13241
                     /gene="ygaZ"
                     /locus_tag="OO335_002746"
     CDS             12504..13241
                     /gene="ygaZ"
                     /locus_tag="OO335_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6003075.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13231..13566
                     /gene="ygaH"
                     /locus_tag="OO335_002747"
     CDS             13231..13566
                     /gene="ygaH"
                     /locus_tag="OO335_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6003076.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13657..14187
                     /gene="emrR"
                     /locus_tag="OO335_002748"
     CDS             13657..14187
                     /gene="emrR"
                     /locus_tag="OO335_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6003077.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14314..15486
                     /gene="emrA"
                     /locus_tag="OO335_002749"
                     /pseudo
     CDS             14314..15486
                     /gene="emrA"
                     /locus_tag="OO335_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15503..17041
                     /gene="emrB"
                     /locus_tag="OO335_002750"
     CDS             15503..17041
                     /gene="emrB"
                     /locus_tag="OO335_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6003078.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="OO335_002751"
     CDS             complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="OO335_002751"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6003079.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="OO335_002752"
     CDS             complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="OO335_002752"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6003080.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19399..19827)
                     /locus_tag="OO335_002753"
     CDS             complement(19399..19827)
                     /locus_tag="OO335_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6003081.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="OO335_002754"
     CDS             complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="OO335_002754"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6003082.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4970 a         5272 c         5145 g         5215 t
ORIGIN      
        1 cagcttacgc ttttactctc atttacgctg ctgaggctgg tatatgttgc atttttagtt
       61 tagagtttac ttaatttaga aaatacttaa ataaatatga ctgaactcgc gcaattacag
      121 gccagtaccg aacaggcagc ggccttattg aaagcaatga gccaccctaa acggttgctg
      181 attctgtgca tgcttagcgg ttcccccggc accagcgcgg gagagctgac gcgcattacc
      241 ggactgagcg cctctgcgac atcacagcat ctcgctcgta tgcgggacga agggcttatc
      301 aacagccaac gggatgccca acgcattcta tattccatta aaaatgaggc ggtaaatgcc
      361 attatcgcca ccctgaaaaa tgtctattgt ccgtaaggag tcactatggc tttgacaacc
      421 atttcgccgc atgatgcaca agaattaatc gcacgcggcg caaagttaat cgatattcgt
      481 gacgctgatg aatatcttcg tgaacatatt cctgaagcag atctggctcc attatccgtg
      541 ctggaacagt caggtcttcc acctaaatta cgtcgcgagc aaattatatt ccactgccag
      601 gcaggtaaac gcaccagtaa taacgccgat aaattagcag cgattgccgc ccccgcagaa
      661 atatttttac tcgaagatgg gattgatggc tggaaaagag ctggattgcc agtagcggta
      721 aataaatctc aacccttgcc gttaatgcgc caggtgcaga tcgctgcggg tggtttaata
      781 ttaaacggcg ttgtactggg ttataccgta aatagcggtt tcttcttatt aagtggcttt
      841 gttggtgccg ggttactgtt tgcaggaatc agcggttttt gtggaatggc aaggttgtta
      901 gataagatgc cgtggaacca acgagcttga gaagcgacgc cggacgcgcc ctagcagcga
      961 catccggcct cagtaattag atcaggaaat cgtcgagaga tttaccttct gccagcgcct
     1021 gagcaattgg cttcggtgta cggccctgac cggtccaggt tttagtttca ccgttaacat
     1081 cagtgaattt atatttcgcc ggacgcggct ggcgtttttt accagcacgt ggtgcagcag
     1141 cagagctatt acccaataac tcttccgggt taattccgtc agctttcatc agctccagcc
     1201 aggtgctaat tttttcctga cgctcagcca gttcacgctg ctgctgttct tcttcttcac
     1261 gtctttcttt agtgacaacc ctgaattttt cgagcatttc ttcaagaacg tcaatggaga
     1321 attcgcgagc catcgcacgg agggtgcgaa tgttatttaa actttgtaac attacggaca
     1381 taataaagaa aaccttttaa cgccaaaaca aaaaagtatt tcgtcgcgta gtgtaagcga
     1441 tcacttatta tatttccaca aaatttaatc tatttacagt cctgcgcgag attatttcag
     1501 ctgttatttt ctgaaactct cagaataatt tcaagattat atattagcca ttttgatgag
     1561 taatttaggt tattggtgaa cagagtggcg ctctctgctc gctaactccg caagcctgac
     1621 aaaaccgaag tgaataaaaa tgcaatataa cagaataaaa gaaaacaccc ctcactggtg
     1681 ctttctgctg ttaatgtaca ttttctggca tcaaaaatgc tgtaaatgac ccggtcatga
     1741 caaacaccga attctctacg cttatagcgt tacctccccc ccaaactttc attagtgaaa
     1801 ttaaaggcaa gatcatatct aattgattta attgaaaata aatcatattg gcatgtaaaa
     1861 atccacaatg tacaaaaaac gagccgttac ggaatatttt atctacaaaa actaactaaa
     1921 taaaaatttt tcactaattg attagtcata gacagcgata tacgctatgc aaaaatgcag
     1981 atggcaatga gatccactgc tttcatctcc attaacatcc cattacgctt ttattaagga
     2041 gcattagcat gttctcaccg cagtcacgct tgcgtcatgc agttgcagat acgttcgcga
     2101 tggttgttta ctgttctgtc gtgaacatgt gtattgaagt tttcctctcc ggaatgagct
     2161 tcgaacagtc tttttattcc agattggtag cgattccggt gaacatctta attgcatggc
     2221 catacggtat gtaccgcgat ctgtttatgc gcgcggcacg caaagttagc ccgtcgggct
     2281 ggataaaaaa tctggctgat atcctggctt atgtgacgtt ccagtcaccg gtgtatgtgg
     2341 cgatcttgtt agtggtgggc gcagactggc atcagattat ggcggcggtc agttcaaaca
     2401 tcgttgtttc gatgttgatg ggggcggttt atggctactt cctcgattat tgccgccgac
     2461 tgtttaaagt cagccgttac cagcaggtaa aagcctgact cttcttttcg cgactggcat
     2521 cgccagtcgc gtctttaact ggcttcgcca aataaaccat tcaaataacg ttcaagcgca
     2581 acacgcgaac taaagccatg cgggataccg taatgctcgt gtcgctcgcc tgctaaatag
     2641 agcggaaatt gctggtaatg atcgcaagtt ttaatatcgg aagcgggttc agccagtgtt
     2701 gaactgcgtt cttttaatgt tggatgaatt accagagcgg tacgtcccat tcgcgcttcg
     2761 cgattaacat agacataatt ttccccacgg cggtaaccat acgctttctg ggttaccaca
     2821 tcgacagtaa aaccgacttt ttcaagtacg cgcgccacct cgtctggtcg taaatacata
     2881 tttgatcctc gttattttca accgcgggcc taccttacct gattgcgcat tcacaacgct
     2941 ttcagaaaag tccataaagc cgcgatgaac ctcagttaag aaaattatgg tctacactga
     3001 aaattacatc gaattctaat gggggatcat atgtttaacc gaccgaaccg caacgacgtt
     3061 gatgatggcg tgcaggatat ccagaatgat gtcaatcaat tagctgacag cctcgaatct
     3121 gtattgaagt cctggggcag cgacgccaaa ggggaagctg aagccgcacg cagtaaagct
     3181 caggcgttgc tgaaagaaac cagggcgcga atgcatggtc gtactcgcgt ccagcaagcc
     3241 gcgcgcgatg ccgttggctg agcggattct tttgttcgtg aaagaccctg gtgtagcgtg
     3301 ggtacagcag ctgcggtagg tatttttatc ggcgcactgt taagcatgcg caaatcgtag
     3361 tgcaaaaatg ataataaata cgcgtctttg accccgaagc ctgtcttcgg ggtttctttt
     3421 tgcctggtga atcacaaaaa tccccctccc ccgtcacgct catatccagg gtaatttcga
     3481 tcactatttg ctatatattg tgtagttgaa tcttttttca actacatcta gtatctctgt
     3541 atcaacagag agacaacccg acgcgtatca tcgcgccgta tcttcatttt aaacggaaat
     3601 acgaatcatg cgcataacta tttatactcg taacgattgc gttcagtgcc acgccaccaa
     3661 acgggcgatg gaaaaccggg gctttgattt tgaaatgatt aatgtcgatc gcgttcctga
     3721 agcagcggaa gcgttgcgtg ctcagggctt tcgtcagttg ccggtagtga ttgctggcga
     3781 tcttagctgg tctggtttcc gtccggacat gattaaccgt ctgcatccag cgccacacgc
     3841 ggccagtgca tgagccagct cgtctacttc tccagcagct ccgaaaacac gcagcgtttt
     3901 atcgaacgtt taggtctgcc cgcggtgcgc atcccgctca atgagcggga acggattcag
     3961 gtagacgagc cttacatcct gatcgtgccc tcttacggcg gcggcggtac ggctggcgcg
     4021 gtgccacgac aggtaattcg ctttttaaac gacgagcaca accgggcgtt gcttcgcggc
     4081 gttattgctt ctggtaatcg caactttggt gaggcgtatg gccgcgccgg agatgtgatt
     4141 gcccggaaat gcggcgtgcc gtggctgtac cgttttgaac tcatgggtac gcaaagcgat
     4201 atcgaaaacg ttcgtaaagg agtaaccgaa ttttggcaac gacaaccgca gaatgcctga
     4261 cgcaggaaac gatggattac cacgcgctga atgcgatgct taatctttac gatagcgcag
     4321 gtcgcattca gttcgataaa gaccgccagg ccgttgacgc ctttattgcg acgcatgtgc
     4381 gtccgaacag tgtgaccttc agtagccagc agcagcgcct gaactggctg gtcaacgaag
     4441 gttactatga tgaaagcgtt cttaatcgct actctcgcga ctttgtcatt acgctgtttg
     4501 cccacgcaca caccagcggt tttcgtttcc agacattcct cggggcatgg aagttttaca
     4561 ccagctatac gttgaagaca ttcgacggta aacgttatct ggaagatttt gccgatcgag
     4621 tcacgatggt ggcgctgacg ctggcacaag gcgatgagac gctggcgttg caactgaccg
     4681 atgaaatgct gtcaggacgc tttcagccag ccacgccaac attcctcaac tgcggtaagc
     4741 agcagcgcgg cgaactggtt tcctgttttt tgctgcgtat tgaagacaat atggagtcga
     4801 ttggtcgggc ggtaaattcc gcactgcagc tgtcgaaacg cggcggcggc gtagcatttt
     4861 tgctgtcgaa tctgcgggaa gcgggcgcgc caattaaacg tattgaaaat caatcttctg
     4921 gcgtaattcc ggtgatgaaa atgttggaag acgcattttc ctatgccaac caactcggcg
     4981 cgcgtcaggg ggcaggtgca gtctatttac atgctcatca tcccgatatt ctgcgttttc
     5041 tcgacacgaa acgggaaaat gccgacgaaa aaatccgcat taaaacactg tcgctgggcg
     5101 tggtgatccc ggatatcact ttccatctgg caaaagagaa tgcgcagatg gcgctgtttt
     5161 cgccctatga cgttgagcga gtttatggca agccgtttgc cgatatcgcc attagcgaac
     5221 actatgacga actggttgcc gatgaacgca tccgcaaaaa atacctcaac gcccgtgatt
     5281 tcttccagcg actggcagaa atccagtttg agtccggcta tccctacatc atgtatgaag
     5341 acacagtaaa ccgtgctaac cctatcgccg ggcgcataaa tatgagtaat ctctgctcag
     5401 aaattttgca ggttaacagc gcctcagagt atgacgagaa tctcgactat gcccgcacag
     5461 gccatgatat ttcctgcaat ttaggttcgt tgaatattgc gcacaccatg gattcccccg
     5521 atttttcccg cacggtagag actgccgtgc gcggtttaac ggcggtgtca gatatgagtc
     5581 atatccgcag cgtgccgtcc atcgaagccg gaaatgccgc ctcgcacgcc atcggactgg
     5641 ggcagatgaa tttacatggt tatctggcga gggaaggcat cgcttatggt tcgccggaag
     5701 ccctggattt caccaatctc tatttctata ccatcacctg gcacgcactg cgtacctcga
     5761 tgttgctggc gcgcgaacgc ggtgaaacct tcgccgggtt caaacagtca cgctatgcca
     5821 gtggtgaata tttcagccaa tatctgcaag gtaactggca gccgaaaacg gcgaaagttg
     5881 gcgaactgtt tgcccgcagc tgtattacgt tacctacccg tgagatgtgg gcgcaactgc
     5941 gcgacgacgt gatgcgctac ggcatataca accagaatct ccaggcggtg ccgccaaccg
     6001 ggtctatctc ttatatcaac catgctacgt cgagtattca tccgattgtg gcgaaagtag
     6061 agatacgcaa agagggcaaa acaggacgcg tttactaccc tgccccgttt atgactaacg
     6121 aaaatctggc gctgtatcag gacgcttacg aaattggcgc agaaaagatc atcgacacct
     6181 acgcggaagc gactcgccat gtcgatcagg ggctgtcgct gacgctgttt ttccccgata
     6241 ccgccaccac tcgcgatatc aacaaagcgc agatttacgc ctggcgcaaa ggtatcaaaa
     6301 cgctctatta catccgcctg cgtcagatgg cgctggaagg cactgaaatt gaaggctgcg
     6361 tctcctgtgc actttaagga atatctatga aactctcacg tatcagcgcc atcaactgga
     6421 acaagatatc tgacgataaa gatctggagg tgtggaatcg cctgaccagc aatttctggc
     6481 taccagaaaa ggtgccgctg tcgaacgata ttcccgcctg gcagacatta actgtcgtag
     6541 aacaacaact gacgatgcgc gtttttactg gcctgacgct gctcgacacg ctgcaaaatg
     6601 ttatcggcgc gccttcgctg atgcccgatg cgctcacgcc tcatgaagag gcggtattat
     6661 cgaatatcag ctttatggaa gcggttcatg cccgctctta cagttcgatt ttctcgacgc
     6721 tgtgccagac caaagatgtc gatgccgcct acgcctggag tgaagaaaac gcaccgttgc
     6781 agcgaaaagc tcagattatt cagcaacatt atcgcggtga tgatccgctg aaaaagaaaa
     6841 tcgccagtgt gtttcttgaa tcttttttgt tctattccgg tttctggctg ccgatgtatt
     6901 tttccagccg cggaaagctg accaataccg cggacctgat ccgtctgatt atccgcgatg
     6961 aagcagtcca cggttactac ataggctata aatatcagaa aaacatggaa aagatatctc
     7021 tgggacaacg tgaagagttg aagagtttcg ccttcgattt gttgctggaa ctctacgaca
     7081 acgagttgca atacaccgat gagctgtacg ccgaaacccc gtgggctgac gatgtgaaag
     7141 cgtttctctg ttacaacacc aataaggctt tgatgaatct gggctacgaa ccgttatttc
     7201 ccgcagaaat ggcggaagtg aatccggcaa tcctcgccgc gctttcgccg aatgccgatg
     7261 aaaatcacga tttcttttcc ggttcaggct cctcttatgt gatggggaaa gcggttgaaa
     7321 cagaagatga agactggaat ttctgagggt gttattttca aaaatatcac tacccgcagc
     7381 agggaaataa ttcccgccaa atagcttttt atcacgcaaa tattctgtag tgatctacac
     7441 tgatactctg ttgcattatt cgcctgaaac tacaatattc aggcgttttt tcgctatcta
     7501 tgacaaaaaa tatcaacttt tctcgatttg ctctcagccc ttatatcacg gaaaattccg
     7561 gcgatttgct cgcatcaata ttcatgccac atttgccctc aggggttgcc tcagattctc
     7621 agtatgttag ggtagaaaaa gtgactattc ccattgggta atatatcgac ataaacaaat
     7681 aaagaaatca ttctattgca tggccattaa attaaaaatt aaaaatcttt ataaaatatt
     7741 tggtgagcat ccacagcgag cgttcaaata tatcgaacaa ggactttcaa aagaacaaat
     7801 tctggaaaaa actgggctat cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg
     7861 cgagatattt gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg tacgccttct
     7921 caatcgcctg attgaaccca cccgcgggca agtgctgatt gatggtgtgg atattgccaa
     7981 aatatccgac gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc
     8041 ctttgcctta atgccgcata tgaccgtgct ggacaatact gcgttcggta tggaattggc
     8101 cggaattaat gccgaagaac gccgggaaaa agcccttgat gcactgcgtc aggtcgggct
     8161 ggaaaattat gcccacagct acccggatga actctctggc gggatgcgtc aacgtgtggg
     8221 attagcccgc gcgttagcga ttaatccgga tatattatta atggacgaag ccttctcggc
     8281 gctcgatcca ttaattcgca ccgagatgca ggatgagctg gtaaaattac aggcgaaaca
     8341 tcagcgcacc attgtcttta tttcccacga tcttgatgaa gccatgcgta ttggcgaccg
     8401 aattgccatt atgcaaaatg gtgaagtggt acaggtcggc acaccggatg aaattctcaa
     8461 taatccggcg aatgattatg tccgtacctt cttccgtggc gttgatatta gtcaggtgtt
     8521 cagtgcgaaa gatattgccc gccggacacc gaatggctta attcgtaaaa ctcctggctt
     8581 cggcccacgt tcggcactga aattattgca ggatgaagat cgcgaatatg gctacgttat
     8641 cgaacgcggt aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac
     8701 gcagcagcaa ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac
     8761 gcctcttagc gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga
     8821 cgaggaccaa cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg
     8881 tgagggggta aataatggct gatcaaaata atccgtggga taccacgcca gcggcggaca
     8941 gtgccgcgca atccgcagac gcctggggta caccgacgac tgcaccggct gacggcggtg
     9001 gtgctgactg gctgaccagt acgcctgcgc caaacgtcga gcattttaat attctcgatc
     9061 cgttccataa aacgctgatc ccgctcgaca gttgggtcac tgaagggatc gactgggttg
     9121 ttacccattt ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat atcctcaacg
     9181 gtttccagca attgctgctg ggtatgccag cgccagtggc gattatcgtt ttcgctctca
     9241 tcacctggca gatttccggg gtcggaatgg gcgtggcgac gctggtctcg ctgattgcca
     9301 tcggcgcaat cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg ttaactgccc
     9361 tgctgttctg tatcgtcatc ggtttgccgt tggggatatg gctggcgaga agtccgcgag
     9421 cggcgaaaat tattcgtcca ctgcttgatg ccatgcagac cacgccagcg tttgtttatc
     9481 tggtgccaat cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca
     9541 tctttgcgct gccgccgatt atccgtctga ccattctggg gattaaccag gttccggcgg
     9601 atctgattga agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc
     9661 agttaccgct ggcgatgccg accattatgg cgggcgttaa ccagacgctg atgctggccc
     9721 tttctatggt ggtcattgcc tcgatgattg ccgtcggcgg gctgggtcag atggtacttc
     9781 gcggtatcgg tcgtctggat atggggcttg ccaccgttgg cggcgtcggg attgtgatcc
     9841 tcgccattat cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca
     9901 accgtcgctg gtacaccact ggccctgttg gtctgctgac ccgcccattc attaagtaac
     9961 tctgcacttg cccggtgacg ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc
    10021 gacatagcgt actttttgcg acagcgtttg ccacgcttat ctctacacaa acttttgctg
    10081 ccgatctgcc gggcaaaggc attactgtta atccagttca gagcaccatc actgaagaaa
    10141 ccttccagac gctgctggtc agtcgtgcgc tggagaaatt aggttatacc gtcaacaaac
    10201 ccagcgaagt ggattacaac gttggctaca cctcgcttgc ttccggcgat gcaaccttca
    10261 ccgccgtgaa ctggacgcca ctccatgaca acatgtacga agctgccggt ggcgataaga
    10321 aattttatcg tgaaggggta tttgttaacg gcgcagcaca gggttacctg atcgataaga
    10381 aaaccgccga ccaatacaaa atcaccaaca tcgcacaact gaaagatccg aagatcgcca
    10441 aactgttcga taccaacggc gacggtaaag cggatttaac cggttgtaac cctggctggg
    10501 gctgcgaagg tgcgatcaac caccagcttg ccgcgtatga actgaccaac accgtgacgc
    10561 ataatcaggg gaactacgca gcgatgatgg ccgacaccat cagtcgctac aaagagggca
    10621 aaccggtgtt ttattacacc tggacgccgt actgggtgag taatgagctg aagccaggga
    10681 aagatgtggt ctggttgcag gtgccgttct ccgcactgcc gggcgataaa aatgccgata
    10741 ccaaactgcc gaatggcgcg aattacggct tcccggtcag caccatgcat atcgttgcca
    10801 acaaagcctg gaccgagaaa aatccggcgg cagccaaact gtttgccatt atgcagttgc
    10861 cagtggccga tattaacgcc cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg
    10921 atattcaggg ccacgttgat ggctggatca aagcccacca gcagcagttc gatggctggg
    10981 tgaatgaggc gctggcagcg cagaagtaat ttttattcgg gcggataagg cgttttcgcc
    11041 tcatccgccg ctctgtacac aatgcctgat gcgacgctga cgcgtcctat catgcctaca
    11101 aaccacatcg cacatttcaa caagctatct ttcatcccat attcatcaac atccgctatt
    11161 attgatttcc agcttaatca tcacctgatg aacaaaaata atgactaaac ctaatcatga
    11221 gcttagcccg gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg tagccagtaa
    11281 ctattacgcc cagccattgc tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc
    11341 ggcaggcttt attgttaccg ccgcgcagtt gggctatgcc gcaggtctac tgtttcttgt
    11401 tcccctcggt gatatgtttg aacgccgccg cctgattgtc tcgatgacct tactggcagc
    11461 gggcggtatg ttaatcactg ccagcagtca gtcactggcg atgatgatcc tcggtacggc
    11521 attaaccggt ttattctcag tcgtggcaca aattctggtt ccgctggcag cgacgctggc
    11581 ttcaccggat aaacgcggca aagtggttgg caccattatg agcggtctgc tgttggggat
    11641 attgctggca cgaaccgttg ccgggttact ggcaaacctc ggcggctggc gcaccgtctt
    11701 ttgggttgct tcggtgttaa tggcactgat ggcgctggca ttatggcgtg gtctgccaca
    11761 aatgaaatca gaaacccacc tcaactaccc acagttgttg ggttccgttt tcagcatgtt
    11821 catcagcgat aagatcctgc gcacccgcgc gttgctgggc tgcatgacct ttgccaactt
    11881 cagcattctc tggacctcaa tggccttttt gcttgccgct ccacctttta actacagcga
    11941 tggtgacatt ggtctgtttg ggcttgcggg agctgccgga gcgttgggcg ctcgtccggc
    12001 gggcggtttt gccgacaagg gcaaatcgca ccacaacttt cggtctgctt ctgctgttac
    12061 tttcatggct ggcaatctgg tttggtcaca cttccgtact ggcgttgatt atcggcattc
    12121 tggtgctgga cctcaccgta cagggcgtgc atatcactaa ccagacggta atttatcgaa
    12181 tacatcctga tgcgcgtaat cgcctgaccg caggttacat gaccagctac tttattggcg
    12241 gtgccgccgg ttcgctaatt tcagcctcag cctggcaaca tggcggttgg gctggcgttt
    12301 gtctggctgg cgcgacgatt gccctggtta acttactggt ctggtggcga ggttttcatc
    12361 gtcaggaagc cgcaaattaa gcaatcagca accgttttcg tggctttaca cttataaggg
    12421 tgttaagaag cccatcagtc tgataaggtt aagatattca ttcagtctat ttataatatt
    12481 aacaatcgtt aagcgtacac tctatggaaa gccctactcc acagcctgct cctggttcgg
    12541 cgaccttcat ggaaggatgc aaagacagtt taccgattgt tattagttat attccggtgg
    12601 cctttgcgtt cggtctgaat gcgacccgtc tgggattctc tcctctcgaa agcgtttttt
    12661 tctcctgcat catttatgca ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg
    12721 ggagtagttt gtgggttgct gcactgagcg tcatggcaat ggatgttcgc catgttttgt
    12781 atggcccgtc actgcgtagc cgtattattc agcgtctgca aaaatcgaaa accgccctat
    12841 gggcgtttgg cctgacggat gaggtttttg ccgccgccac cgcaaaactg gtacgcaata
    12901 atcgccgctg gagcgagaac tggatgatcg gcattgcctt cagttcatgg tcatcgtggg
    12961 tatttggtac ggtaataggg gcattctccg gcagcggctt gctgcaaggt tatcccgccg
    13021 ttgaagctgc attaggtttt atgcttccgg cactctttat gagtttcctg ctcgcctctt
    13081 tccagcgcaa acaatctctt tgcgttaccg cagctttagt tggtgccctt gcaggcgtaa
    13141 cgctattttc tattcccgtc gccattctgg caggcattgt ctgtggctgc ctcactgcgt
    13201 taatccaggc attctggcaa ggagcgcccg atgagctatg aggttctgct gcttgggtta
    13261 ctggttggcg cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat
    13321 gcccgcccaa ccaaacgtgg cgcggtaggt attttgctcg acaccattgg catcgcctcg
    13381 atatgcgctc tgctggttgt ctctaccgca ccagaagtga tgcacgatac acgccgtttc
    13441 gtgcccacgc tggtcggctt cgcggtactg ggtgccagtt tctataaaac acgcagcatt
    13501 atcatcccaa cactgcttag tgcgctggcc tatgggctcg cctggaaagt gatggcgatt
    13561 atataaccca caagaatcat ttttctaaaa caatacattt actttatttg tcactgtcgt
    13621 tactatatcg gctgaaatta atgaggtcat acccaaatgg atagttcgtt tacgcccatt
    13681 gaacaaatgc taaaatttcg cgccagccgc cacgaagatt ttccttatca ggagatcctt
    13741 ctgactcgtc tttgcatgca catgcaaagc aagctgctgg agaaccgcaa taaaatgctg
    13801 aaggctcagg ggattaacga gacgttgttt atggcgttga ttacgctgga gtctcaggaa
    13861 aaccacagta ttcagccttc tgaattaagt tgtgctcttg gatcatcccg taccaacgcg
    13921 acgcgtattg ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtaa aagcgataac
    13981 gatcgccgct gcctgcatct gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt
    14041 ttaccaccgc agcataactg cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa
    14101 gatcagctcg agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac
    14161 ggtgtggttc tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc agatttataa
    14221 aagaaaaaat gaccggccag catcgcaaca tgctggcctt tttggcaagc aggtcggctc
    14281 agccgatgag ttaagaagat cgtggagaac aatatgagcg caaatgcgga gactcaaacc
    14341 ccgcagcaac cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg
    14401 ctctttataa ttattgccgt agcgataggg atttattggt ttttggtact gcgtcatttc
    14461 gaagaaaccg atgacgcata cgtggcaggg aatcagattc aaattatgtc tcaggtgtct
    14521 ggcagcgtga cgaaagtctg ggccgataac accgattttg taaaagaagg cgatgttctg
    14581 gtcactctcg acccgacaga tgctcgccag gcgtttgaaa aagccaaaac tgcactggct
    14641 tccagcgttc gccaaaccca ccagctgatg attaacagca aacagttgca ggcgaatatt
    14701 gaggtgcaaa aaatcgccct cgcgaaagca caaagcgact acaaccgccg tgtgccgctg
    14761 ggcaatgcca acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt
    14821 gcccaggcgc aactggacgt cgctattcaa caatacaatg ccaatcaggc gatgattctg
    14881 gggactaaac tggaagatca gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc
    14941 tagctggcgc tggagcgtac ccgtattgtc agtccgatga ccggttatgt ttcccgtcgt
    15001 gccgtgcaac cgggtgcgca aattagccca acgacgccgc tgatggcggt cgttccagcc
    15061 accaatatgt gggtggatgc caactttaaa gagacgcaga ttgccaatat gcgcatcggt
    15121 cagccggtca ccatcaccac cgatatttac ggcgatgatg tgaaatacac cggtaaagtg
    15181 gttggtctgg atatgggcac aggtagcgcg ttctcactgc ttccggcgca aaatgcgacc
    15241 ggtaactgga tcaaagtcgt tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag
    15301 ctggagcaat acccgctgcg cattggtttg tccacgctgg tgagcgtcaa taccactaac
    15361 cgtgacggtc aggtactggc aaataaagta cgttcaacac cggtcgcggt aagtaccgcg
    15421 cgtgaaatca gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct
    15481 ggctaatcca gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt
    15541 cattatgacg attgcgctgt cactggcgac attcatgcag gtgctgaact ccaccattgc
    15601 taacgtggcg atccccacta tcgccgggaa tctgggctca tcgctcagcc aaggaacgtg
    15661 ggtaatcact tctttcgggg tggcgaatgc catctcgatc ccgcttaccg gctggctggc
    15721 aaagcgcgtc ggggaagtga aactgttcct ttggtccacc atcgcctttg ctattgcgtc
    15781 ttgggcgtgt ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg
    15841 gattgtcgcc gggccgttga tcccgctttc gcaaagtcta ttgctgaata actacccgcc
    15901 agccaaacgc tcgatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg
    15961 cggcccgatc ctcggcggtt atatcagcga taattaccac tggggctgga tattcttcat
    16021 caacgtgccg attggcgtgg cggtggtgtt gatgacactg caaaccctgc gcggacgtga
    16081 aacccgcacc gaacggcggc ggattgatgc cgtggggctg gcactgctgg ttattggtat
    16141 cggcagcctg cagattatgc tcgaccgcgg taaagagctg gactgttttt catcacagga
    16201 aattatcatt cttaccgtgg tggcggtggt ggctatctgc ttcctgattg tctgggagct
    16261 gaccgacgat aacccgatag tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg
    16321 ctgcttgtgt atcagcctcg cgtatatgct ctacttcggc gctattgttc tgctgccgca
    16381 gttgttgcag gaggtctacg gttacacggc gacctgggca ggtttggcct ctgcgccggt
    16441 agggattatt ccggtgattc tgtcgccgat tatcggccgc ttcgcgcata aactggatat
    16501 gcggcggctg gtaaccttca gctttattat gtatgccgtc tgcttctact ggcgtgccta
    16561 tacctttgaa ccaggtatgg atttttgcgc gtcggcctgg ccgcagttta tccaggggtt
    16621 tgcggtggtc tgcttcttta tgccgctgac caccattacg ctgtctggtt tgccaccgga
    16681 acgactggcg gcggcatcga gcctctctaa ctttacgcga acgctggcgg ggtctatcgg
    16741 cacgtcgata accacgacca tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac
    16801 tgagtcggta aacccgttca acccgaatgc ccaggcgatg tacagtcaac tggaagggct
    16861 tgggatgacg caacagcagg catcaggctg gattgcccag cagatcacca atcaggggct
    16921 gattatttcc gccaatgaga tcttctggat gtcagccggg atattcctcg tactgctggg
    16981 gctggtgtgg tttgctaaac cgccatttgg cgcaggtggc ggcggaggcg gtgcgcacta
    17041 agtacaacta agccagttca tttgaactgg cttttttcaa ttaattgtga agatagttta
    17101 ctgactagat gtgcagttcc tgcaacttct ctttcggcag tgccagttct tcgttgctgt
    17161 tgatgcgtac ctcacgttcc agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt
    17221 gcatctggta agtgccacac tggtagacgt tcagttccgg gatctgattc tgatcctgca
    17281 ctttcagcac gtcttccatt gccgctttcc aggcatcagc aacacgctgc tcatctggcg
    17341 taccaatcag actcatataa aaaccggtgc ggcagcccat tggcgagata tcgataatct
    17401 ctacaccatt accgttaaga tggttacgca taaaaccagc aaacaggtgc tccagggtat
    17461 ggatccctct ttctggcatc acttctttgt tcggcacgca gaagcgcaga tcgaacacgg
    17521 tgattgcgtc gccatgcggg gtgttcattg ttttcgccac ccgaactgca ggcgcttcca
    17581 tccgggtatg atcgactgtg aagctatcta acaacggcat ttagccacct ccggtaattt
    17641 ttttaaaaat tttctgaact ctttcttccc aggcgagtct gagtatatga aagacgcgca
    17701 tttgttatca tcatccctga attcagagat gaaattttgg ccactcccga gtggcctttt
    17761 tcttttctgt caggcgtgtt tttccagcca caccgcaaac ggttcggtat cagcggtttc
    17821 catttcctgc tgacggcgtt cagatgcctc gcgctcggct acaaaatcct cttcgcgcag
    17881 aatttccagc ggctcttcac gcagcagatt acggtaggct tctgcaaacg ctttgcctgt
    17941 tccgccaata ccagtatcaa tcatagacct taagatacgg gcagagaaag tcagatcggg
    18001 attatcgaag caggcaacca gttcatcaca cactttctga tacgcttcgc cgccgttaat
    18061 gctatccagc gtttgcgcga cgcgtttcag atcgcggaac agatctttac ccacctgcag
    18121 taacgggaac tgcgcggttt cgcagccgat acccagcgtc aggcccggtt tgcgaccttc
    18181 aaggatcacc cggttccagt taacgcgcgt acaggcaagt tcgctactgc tcatttccgg
    18241 tgcatcagcc agcgcacacc agaccataaa caggtcgagg aatcgcacct gctgttcatc
    18301 tacaccaatc ggcgagaacg ggttgatgtc cagcgaacgc acttcaatat attcaatgcc
    18361 gccacgtaac agcgcatcag aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat
    18421 cggcgcatac agttcgtttt caatctgcag cacgttgcta ttgatttgca gcctcttacc
    18481 gtctttctca ataccaatct tcgcgtactc ttccgatggc gttttgattg cctgtttaag
    18541 gcccgctacg tactcgtaaa gatcgttgaa ggtaatacca agattgcttt gcgatttatt
    18601 ggtatagccg agatcgctca aacgaagaga ggtcgcatac ggcaggtaat acataccgca
    18661 ctcggttttc tcaaacggca gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc
    18721 cggagatgca ccaaacagat aaggaataac ccaaccgaaa cgatagtaat tgcggataac
    18781 gcggaaatag cccgcagaaa ttttctcttt ggcatcagcg cccgagatat caccgcactt
    18841 cgcttgccag aatgccattg gcaaagagaa attgtagtgc acgccggaaa tggtttgcat
    18901 cagcgcgccg tagcgatttt tcagcccttc acgatacagc gttttaaagc gtccggtgtt
    18961 agaagtgccg tactgtgcca gttcgatgtc ctgaccttct gcgatgtagc atggcatact
    19021 taacggccac atccgctcat cgcccatatt gcgcgccgta taacgatgca gatcgcgcat
    19081 aaaggtcagc atatgttcaa tatcaccatc cactggtgta atgaattcca gcaatgcttc
    19141 cgcaaaatcg gtagtaatcc atttgtgcgt cagtgcggaa cctaatgctt caggatgacc
    19201 tgttgttgcc agtgtgccat cagcattaac acgcaaagtt tcgcgctcca gcccacgctg
    19261 tatccccttt aacgcctgag gatgtttttc cagccaggcc agcgcctgtg atacgtccgg
    19321 gatcaaattg acctcccgcc tgtcaaaatc gttttaatta gcataactgt aatggtgacc
    19381 atatgtgcag gcctacaatt agtgccacca catcatgccc tgaacggtcg ctgctgcaac
    19441 tgcaacatag cgtaacgctt taccaaggca caaaaaaaag attactggcc cccaggagat
    19501 gcgcatccat cccgctaaca gacacagtaa atcgccaacc acgggcatcc agcttaataa
    19561 tagcgtgact gcaccatagc gtttcagcca gccggtagct ttctcttgcc agcgcgatgt
    19621 tttacgcaat ggaaagaaac gcccaaggat aacgttagtt aaccctccaa ggctattacc
    19681 cattgttgct gttaaaacta aaacccaggg atgactgatc ccggaaagca acattgccac
    19741 cagcacgact tcggagttgc cgggtaatag tgtagcgctg agaaaactac tggcaaacaa
    19801 cgagaaaagc gataacgctt cactcacagc aagcgaacat ccacagcgtc catgcctgct
    19861 gcacgggccg cctgaatacc gaaatcggca tcttcaaaga ccacacactg cgtcggttgc
    19921 acgcccatac gctgcgcgca caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg
    19981 tgatcagcag cgacgacggc gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc
    20041 tcagcgatgg cgctttcact ccccgttcct acagccattg ggcgacgacc atgccaactt
    20101 ttcaccactt caacaagagg aagcggctcg acactatcca gcagcatact tcttactgct
    20161 tctgtttttt cacgcgctaa cgcatgcggg tcgagatcgg cctgattcag ctcaataatt
    20221 gcctgagcaa tacgccaggt gggcgatcca ttaagcgcaa tcatcgcctg aatatcgtac
    20281 tgaagaccgt agtgccctaa tacttcgcgc cacgctttac ggtgcgtagg cgccgtatcc
    20341 aggattgtgc catccatatc aaaaattaaa cctgcataac gctcgtacat ggtcttctcg
    20401 caagtcgaaa aatatgacgc tactttagcg taattgcttg attttgtcgc tgatggcgaa
    20461 ggagaagaaa attcaggaaa tggataaagt agttatttag aaatgagata tttttgaagg
    20521 aaattttttg gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt
    20581 agccgagtac tctatccagc tg
//
LOCUS       DAJTSF010000073        20542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000073 DAJTSF010000000
VERSION     DAJTSF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.073"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="OO335_002755"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="OO335_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6003083.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="OO335_002756"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="OO335_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR6003084.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="OO335_002757"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="OO335_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6003085.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO335_002758"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO335_002758"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6003086.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO335_002759"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO335_002759"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6003087.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIAGSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="OO335_002760"
     CDS             4449..4814
                     /locus_tag="OO335_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6003088.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="OO335_002761"
     CDS             4964..5482
                     /locus_tag="OO335_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR6003089.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="OO335_002762"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="OO335_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="OO335_002763"
     CDS             6713..7195
                     /locus_tag="OO335_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6003090.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OO335_002764"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OO335_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6003091.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OO335_002765"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OO335_002765"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6003092.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OO335_002766"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OO335_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6003093.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OO335_002767"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OO335_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6003094.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OO335_002768"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OO335_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6003095.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="OO335_002769"
     CDS             10939..11730
                     /locus_tag="OO335_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6003096.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="OO335_002770"
     CDS             11751..13037
                     /locus_tag="OO335_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6003097.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OO335_002771"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OO335_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6003098.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="OO335_002772"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="OO335_002772"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6003099.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="OO335_002773"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="OO335_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6003100.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="OO335_002774"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="OO335_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6003101.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="OO335_002775"
     CDS             complement(16985..17275)
                     /locus_tag="OO335_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6003102.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OO335_002776"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OO335_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6003103.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="OO335_002777"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="OO335_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6003104.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="OO335_002778"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="OO335_002778"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="OO335_002779"
     CDS             19137..20378
                     /locus_tag="OO335_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6003105.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5305 a         5365 c         4819 g         5053 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatttgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagccagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acaccccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attgcaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccaa gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgttgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtatttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 tcggtaatgc tgccaactta ct
//
LOCUS       DAJTSF010000074        20288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000074 DAJTSF010000000
VERSION     DAJTSF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.074"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="OO335_002780"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="OO335_002780"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6003106.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OO335_002781"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OO335_002781"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR6003107.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..3000)
                     /locus_tag="OO335_002782"
                     /pseudo
     CDS             complement(1728..3000)
                     /locus_tag="OO335_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3183..4388)
                     /locus_tag="OO335_002783"
     CDS             complement(3183..4388)
                     /locus_tag="OO335_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6003108.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4488..4844)
                     /locus_tag="OO335_002784"
     CDS             complement(4488..4844)
                     /locus_tag="OO335_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR6003109.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4980..5087)
                     /gene="rprA"
                     /locus_tag="OO335_002785"
     ncRNA           complement(4980..5087)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO335_002785"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="OO335_002786"
     CDS             complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="OO335_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6003110.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6774..9830
                     /locus_tag="OO335_002787"
     CDS             6774..9830
                     /locus_tag="OO335_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6003111.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9827..10237
                     /gene="menI"
                     /locus_tag="OO335_002788"
     CDS             9827..10237
                     /gene="menI"
                     /locus_tag="OO335_002788"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6003112.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10337..10525
                     /locus_tag="OO335_002789"
     CDS             10337..10525
                     /locus_tag="OO335_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6003113.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11073..11441
                     /gene="sufA"
                     /locus_tag="OO335_002790"
     CDS             11073..11441
                     /gene="sufA"
                     /locus_tag="OO335_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6003114.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11450..12937
                     /gene="sufB"
                     /locus_tag="OO335_002791"
     CDS             11450..12937
                     /gene="sufB"
                     /locus_tag="OO335_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6003115.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12947..13693
                     /gene="sufC"
                     /locus_tag="OO335_002792"
     CDS             12947..13693
                     /gene="sufC"
                     /locus_tag="OO335_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6003116.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13668..14939
                     /gene="sufD"
                     /locus_tag="OO335_002793"
     CDS             13668..14939
                     /gene="sufD"
                     /locus_tag="OO335_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6003117.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14936..16156
                     /gene="sufS"
                     /locus_tag="OO335_002794"
     CDS             14936..16156
                     /gene="sufS"
                     /locus_tag="OO335_002794"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6003118.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16169..16585
                     /gene="sufE"
                     /locus_tag="OO335_002795"
     CDS             16169..16585
                     /gene="sufE"
                     /locus_tag="OO335_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6003119.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16734..17738
                     /gene="ldtE"
                     /locus_tag="OO335_002796"
     CDS             16734..17738
                     /gene="ldtE"
                     /locus_tag="OO335_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6003120.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="OO335_002797"
     CDS             complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="OO335_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6003121.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18348..19760)
                     /gene="pykF"
                     /locus_tag="OO335_002798"
     CDS             complement(18348..19760)
                     /gene="pykF"
                     /locus_tag="OO335_002798"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6003122.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5316 a         4862 c         5177 g         4933 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaaaggt tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgacag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgtactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagccatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgtcaaa aaagaaggcc
     4141 atatagcagc acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgcca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaattaaaa tacaacccaa gtgcggtagg gtaataggga
     4381 tttttcatag gttctactta gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta
     4441 tccattcatt attgttgtaa caacgacaat tcctttctgt ggaaaaatta acaaagccgc
     4501 tccagatcgt gtgcatataa ttcagcggct accgattgat agatcttcca gtcaataaaa
     4561 ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg
     4621 ttgccgatgc ggttatttat tttctctatg atggcatgaa gatgtttttt tcgttgctga
     4681 cgcatagcca acagttgctc gactacacaa tcaggaatgg cgcatttgcc attttcccac
     4741 tgacgccagc tttcactgtc accggtctgg gcaatccagg tcgcgcattc atcaatagtc
     4801 atggcaaaaa tatggcggag tgcctggagt tcataagcgt tcatcgtgta atggctgcat
     4861 gaaataaaca ataaaatata aataacatta tcagcctgct gacggcttga agagagtcac
     4921 agtatcttgt gcaacattat tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt
     4981 taaaaaaaag cccatcgtgg gagatgggaa aagactacac acagcaattc gttgtttcac
     5041 tcaggggatt tccatgctta taaatcaata tgttgattta taaccgtgag ctaatagtag
     5101 gcatacggac tttttgcgtc gatcagattc gtctcattag tttaaaaact gtaaagaatt
     5161 tgcgacaggt actaccagct gaatggcggt aagcaggaaa atatggggaa gagcaagcaa
     5221 atatcaatta gcggtgatta acaatcaggc ggcagagccg cctgatgaga aattacttat
     5281 tcggtttctc gatttcgcca agttcttcga gaatttcttc cggttggtcc gtcggtggcg
     5341 ggacttcttc cacccacgcg gcaaacagac gccaggaaac ggctaacaga accggaccaa
     5401 taaacagacc gatcatcccg aaagcaatca aaccaccaat aacgccagag aggatcagga
     5461 tcagcggtaa atcggcaccc atgcgaatta acattgggcg gatgacgtta tccagcgtgc
     5521 caaccacacc gctccacact aacaataccg ttccccaggt ggtatcgccg gtccagtaga
     5581 gccagataat cgccggaatc agtaccggca acgggccaag ctggacaagg caggagagga
     5641 tcattaacac cgttagcaac gttgcataag gtacgccgga tacggcgagg ccgataccgc
     5701 caagcactgc ctgtactaac gccgtcacca ccacacccag cgccaccgcg cggatagcct
     5761 gtgccgccag cagcacggcg gcatctccgc gaacgcctgc cagacgggtt gcaaaatggc
     5821 gaatgccttg tgccacctgt tcaccgcgcc agtacagcag ggcactgaag agaagcatca
     5881 gcgcacaatg caccataaag cgcccgatat gcgccgcctg cccaacgaac caggtggtgg
     5941 tggtgccaat ataagggcgg actttcgcca tgatcgccgt accccccata tccagcaagt
     6001 tgtgccagcc tgcatacagc ttcgcgccaa tcaccggaat ggtattaagc cacgccagat
     6061 cgggtaacgt catgtcgccg ctggaaatgg ctttaattag cgggccgctg ccgtcgacga
     6121 tactgttaac cagcaaagca atagggatga taaacaccat cactaataac agcgtcatca
     6181 ccagaacggc gagagagcgg cggccaaaca tgatcttttg caaacgtaac aataccggcc
     6241 aggtggcgat aaccaccgta ccggcccatg caaagccgag aataaagggt tgaacaatcc
     6301 acagacatgc cacaatcatg atggctaaaa acagcaccga aagcagaatt tgtgcgacat
     6361 ccctgggctg acgaacattt accataacta cttttcacct tttttgtgcg tcaaatcgtc
     6421 gacgcgacgt gaacacgcga aactcacctg cataatgatg agcaatttca gcggttttta
     6481 acaggccgat tctgccctta attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac
     6541 tcaggcaaca cgcaaacgat ttactcgtcg tatttcaagt tgcatgtgct gcggctacgt
     6601 tcgttcaccc cagtcactta ctattgtaag ctcctggagt tcactcgctt gccgccttcc
     6661 tgcaactcga aatccataga gtaaataaac atcacctttc agtgcgctat tgcagccgcc
     6721 tgaaagaata gtgtatcgac ctacatcaca gaccgcagga aagggtcaat ataatgattc
     6781 cacagatttc cctggcaccc ggcgtcgttc aactggtgct taattttttg caagagctgg
     6841 agcaacaagg ttttaccggc gatgcggcga caagttatgc cgatcgtctg acaatgtcga
     6901 ccgacaacag tatttaccaa cttctccccg atgcggtggt atttccgcgt tcaaccgcag
     6961 atgtggcgct gatcgcccgt cttgccgcgc aggaacgcta ttcatcgctg atctttaccc
     7021 cccgcggcgg cggcaccggc actaacggtc aggcgctcaa ccaggggatt attgttgata
     7081 tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg
     7141 aggccggggt gataaaagac caactcaatc agtacctgaa accgttcggc tacttttttg
     7201 cgccggaact ttcgaccagc aaccgggcaa cgctcggtgg gatgatcaat accgatgcat
     7261 ccggtcaggg atcgctggtc tatggcaaaa cgtcagatca cgtacttggc gtacgcgcgg
     7321 tgttgttggg gggcgatatt ctcgatacgc aacctttacc cgtcgagctg gcggaaacgc
     7381 tgggtaaatc caataccaca atcgggcgaa tttataacac ggtttatcaa cgttgccgtc
     7441 agcaacgcca gttaatcatc gacaacttcc ccaaacttaa ccgctttctt accggttacg
     7501 atctgcgtca tgtctttaac gatgagatga ccgagttcga cctgacgcgc attctgacgg
     7561 gttcagaagg gacgctggcc tttattaccg aagcgcggct ggatattacg cgcttgccta
     7621 aagtgcgccg tctggtgaat gtcaaatatg actcttttga ctctgcgctg cgtaacgcgc
     7681 cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc
     7741 tggcgcggga agatattgtc tggcattccg tcagcgagtt gattaccgat atgcctgaca
     7801 aagagatgct cggactgaac attgtggaat ttgctggtga tgatgaggcg ctgattgacg
     7861 agcgagttaa tgcactctgt gtgcggcttg atgagctgat tgccagccaa caggcaggtg
     7921 tgattggctg gcaggtgtgc cgcgagctgg cgggggttga acgtatctat gcgatgcgca
     7981 aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg
     8041 aggatacctg cgtaccgccg gaacatctgg ccgattatat tgctgaattt cgcgcgctgc
     8101 tcgacagcca cggcttaagc tacggtatgt tcggtcacgt cgacgcaggt gtcttgcacg
     8161 tccgtccggc actggatatg tgcgatccgc aacaagagat tttgatgaag caaatctctg
     8221 atgacgtggt ggcgctgact gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag
     8281 gttttcgcgc tgaatacagc ccggcgtttt tcggtgagga actttttgca gaactgcgca
     8341 aagtgaaagc ggcatttgac ccgcataacc gactcaaccc agggaagatt tgcccgccag
     8401 aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc
     8461 agatccccat tgcggtacgc cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt
     8521 tatgcttcaa ctttgatgcc cgtagtccga tgtgtccgtc gatgaagatc acccagaacc
     8581 ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga atggctgcgc ttgttggcgg
     8641 atcgcggcgt tgacccactc aaactggaac aagaactgcc tgaatccggc gtcagtttgc
     8701 ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct
     8761 cacacgaagt caaagaggcg atgtcgggct gtctggcctg taaagcttgt tcgacccagt
     8821 gccccatcaa aattgatgtg ccggagtttc gctctcgttt tctgcagctc tatcacaccc
     8881 gttatttacg cccgcttcgc gaccacctcg ttgctacggt cgagagctac gcaccgctga
     8941 tggcacgcgc gccgaaaacc tttaacttct tcattaacca gccgctggtg cgcaaactct
     9001 cggaaaaaca tatcggcatg gttgatttgc cactgctgtc ggtcccctcg ctacaacaac
     9061 aaatggtggg gcatcgctcg gcaaacatga cgctggaaca gcttgaagct ctcaatgcag
     9121 agcagaaagc gcgcacggtg ttggtggtgc aggacccctt taccagctat tacgatgcgc
     9181 aagtggtggc ggattttgtc cgtctggttg aaaaattagg tttccagcct gtgttactgc
     9241 cattttcgcc aaatggcaaa gcccagcata tcaaaggctt tcttaatcgt tttgcgaaga
     9301 cggcgaaaaa gacggcggat ttcctcaacc gtatggctaa gcttgggatg ccaatggtgg
     9361 gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata taaactggcg ctgggcgagg
     9421 aacgtggcga gtttaacgtc ttacttgcga atgaatggct ggcaagcgca cttgagtcac
     9481 atccagtggc tacagtcagc ggtgaatcat ggtatttctt tggtcactgt accgaggtta
     9541 ccgctttgcc gggcgcgcca gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac
     9601 tggaaaatgt cagcgtgggt tgctgcggca tggcagggac ttacggacat gaagcgaaaa
     9661 accatgaaaa ttcgctcggg atctatgagt tatcctggca tcaggctatg cagcgactgc
     9721 cgcgtaaccg ctgtctggcg accggatatt cctgccgtag ccaggtaaaa cgggttgaag
     9781 gcacgggggt acgccatcct gtgcaggctt tactggagat tattaaatga tatggaaacg
     9841 gaaaatcacc ctggaagcac tgaatgctat gggtgaagga aacatggtgg ggttcctgga
     9901 tattcgcttt gaacatattg gtgatgacac ccttgaagcg acaatgccag tagactcgcg
     9961 gacaaagcag cctttcgggt tgctgcatgg aggagcatcc gtggtactgg ccgaaagtat
    10021 cggttccgtt gccggttatt tatgtaccga aggtgagcaa aaagtggttg gtctggaaat
    10081 caatgctaat cacgtccgct cggcacgaga agggcgggtg cgcggcatat gtaaaccgtt
    10141 gcatctcggt tcgcgtcacc aggtctggca gattgaaatc ttcgatgaga aagggcgttt
    10201 gtgctgttcg tcacgattga cgaccgccat tttgtgacgt tgtcaccaga aaagtgtgac
    10261 gcatattgct tttggtcatt atctgcacag taaacggtgg tatactgtgc aggtcttgtc
    10321 taaaagcgag gatgctatgt ctactcaact tgatcccacc cagctggcca ttgaattttt
    10381 acgccgtgac cagagcaatc tttcacctgc ccaatatctc aaacgtctga aacaactaga
    10441 acttgaattt gctgacctgc ttactctctc ttctgctgaa ttaaaagaag aaatctactt
    10501 cgcctggcgt ctgggcgtgc attgatcctg acttaatgcc gcaagttcgc caaatcgacc
    10561 tggattaacc tccgtccagg ttcgatgacc ttcttaatac tgcttatcta ccgcctgata
    10621 ttgttcaaaa ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat
    10681 tattcttatc gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg
    10741 gggtagaaac ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct
    10801 gcactttttt acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac
    10861 agccttaatg ctccttagat aataaccatt atctaacaat gagataccta attcttagcg
    10921 tgcctgttaa cccacacatc aggggctatg cttattaaaa gcaggcaaaa agaatgtttt
    10981 acggtaaagc ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt
    11041 atacgctgaa agcgatgaag tgaggtaaat agatggacat gcattcagga acctttaacc
    11101 cacaggattt cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgcg
    11161 agctggtggc aaagcagcca ggtatggtcg gcgtgcgctt aggtgtgaag caaacgggct
    11221 gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt
    11281 ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaatc gatgccgttt attgatggca
    11341 cggaagtcga tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag
    11401 cccagaatga atgtggctgt ggcgaaagct ttagggtata ggcggtacta tgtctcgtaa
    11461 tactgaagca actgacgatg tcaaaacctg gaccggcggc ccgctgaact ataaagaagg
    11521 attcttcacc cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg
    11581 cgcaatttcg gcgaagcgta atgagccgga gtggatgctg gagtttcgtc taaacgccta
    11641 tcgcgcatgg ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa
    11701 ttatcaggat tacagctact actcagcacc atcgtgcggt aattgtgacg acacttgcgc
    11761 gtctgaacct ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga
    11821 ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc
    11881 cattttcgac tctgtttcgg ttgccaccac ttatcgcgaa aaactggcgg agcagggaat
    11941 tattttctgt tcctttggtg aggcgatcca cgatcacccg gaactggtgc gtaaatatct
    12001 cggcaccgtg gtgccgggga atgacaactt ctttgcagca cttaatgcgg cggtagcctc
    12061 tgacggtacg tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta
    12121 ttttcgcatt aacgcggaaa aaaccgggca gtttgagcgc acaattctgg tggccgacga
    12181 agacagctac gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt
    12241 gcacgcggca gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt
    12301 acaaaactgg ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg
    12361 tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc
    12421 gattacgtgg aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta
    12481 ctcagtggcg ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat
    12541 cggtaaaaac accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa
    12601 cagttatcgc ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca
    12661 gtgcgactca atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg
    12721 tcgcaataac agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca
    12781 actgttttac tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa
    12841 cggtttctgc aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa
    12901 actcctcgcc atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa
    12961 agatttacac gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg
    13021 tcccggcgaa gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc
    13081 aacgcttgcc gggcgcgaag attatgaagt gacgggcggc acggttgagt tcaaaggcaa
    13141 agatttgctt gcactgtcgc cggaagatcg tgcaggcgaa ggcatcttta tggccttcca
    13201 gtatccggtg gagattccag gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc
    13261 agtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga
    13321 agagaaaatc gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg
    13381 tttttccggc ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga
    13441 gttatgcatt cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggtcgc
    13501 cgatggcgtg aactcgctgc gtgatggcaa gcgctcattc atcattgtta cgcactacca
    13561 acgcattctc gactacatca agcctgatta cgttcatgtg ctatatcagg gacgaattgt
    13621 gaaatccggc gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac
    13681 cgaacagcag taatgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac
    13741 gctcaccgca agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta
    13801 aacatgaaaa ctggaaatat acgccgctgg aagggctgac caatagtcag tttgtcagca
    13861 ttgcgggaga tatatcccca cagcagcgtg atgccttagc gttaacgtta gacgccgtgc
    13921 ggctggtgtt tgtcgatgga cgttacgtgc ccgcgctgag cgatgcaatt gaaggcagcg
    13981 gatatgaagt gagcattaac gacgaccgtc aggggttacc cgacgctatt caggcggaag
    14041 tgtttctgca tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg
    14101 gtcaacggcc ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg gcaggtgaag
    14161 aggtgaacac tgcccattac cggcatcatc tggatctggc ggaaggtgcc gaagcaacgg
    14221 tgatcgaaca ttttgtcagc cttaatgatg ctcgtcattt taccggggca cggttcacta
    14281 tcaatgtcgc agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccggtca
    14341 gtcaccactt tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccata
    14401 gtttcctgct gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa
    14461 acagcacgct gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc
    14521 gtacctggct ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg
    14581 tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca
    14641 aaacggatgg tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata
    14701 caaaaccgca gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg
    14761 ggcgtattga tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg
    14821 cccagcagat gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg
    14881 ggcttaaaca gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac
    14941 tttttccgtc gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt
    15001 gccgctggct tatctcgaca gcgccgccag tgcacagaaa ccgagccagg tgattgacgc
    15061 cgaggccgag ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag
    15121 cgcccaggcg accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc
    15181 ccgttcggcg gaagagctgg tgtttgtccg cggcacgacg gaagggatca atctggtcgc
    15241 caatagctgg ggcaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga
    15301 gcaccacgct aacattgtcc cctggcagat gctttgcgca cgcgttggcg cagagctgcg
    15361 tgtgatccct ctcaaccccg acggtacgct gcaactggag acgctgccta cgctgtttga
    15421 tgagaaaact cgcctgctgg caattactca tgtctccaac gtgcttggca cagaaaatcc
    15481 actggcggaa atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc
    15541 tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg gattgcgact tttacgtgtt
    15601 ctccgggcat aaactgtatg gccccaccgg aattggcatt ctttatgtga aagaagcctt
    15661 gttgcaggag atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag
    15721 tgaaggcact acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg
    15781 gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaatagcat
    15841 agccgagtat gaacagaatc tgatgcatta tgcgctatca cagctggaat ctgtaccgga
    15901 tctcactctc tatggccaac aaaacaggct tggcgttatt gcttttaatc tcggtaaaca
    15961 ccacgcctat gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca
    16021 tcactgcgct atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcgtcgct
    16081 ggccatgtat aacacccatg aagaagtgga gcgtctggtg accggcctgc aacgtattca
    16141 ccgtctgctg ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct
    16201 gcgtaatttt ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagctggg
    16261 ccagcgtctg ccagaattac gcgccgaaga cggaagccca caaaatagca ttcagggctg
    16321 ccagagtcag gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg
    16381 cgacagcgat gcggcgattg tgaaagggct tattgcggtc gtctttattc tctacgatca
    16441 gatgacgccg caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct
    16501 cacccaacat ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg
    16561 cgccaaagcc gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat
    16621 tgccggatgg tcaatgttta cactgaccgg cacaggagtg ttttggtttc aggtgaacat
    16681 aagcaccatc cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac
    16741 gcgcgtcttt gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg
    16801 cggttgatta tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata
    16861 cggtgcaaga aggggataaa aaccttcagg ctattgcccg acgttttgat actgcagcaa
    16921 tgttgatcct tgaagccaat aacactatcg ccccggtgcc aaaacctggt acgacgataa
    16981 ctattccttc acaactgtta ttacctgatg caccgcgtca ggggattatc gttaaccttg
    17041 cagagctgcg cctttattat tatccgccgg gagaaaatat tgtgcaggtt tatccaatag
    17101 gtattggatt gcaggggctg gaaacgccgg tgatggaaac gcgtgttggg cagaaaatcc
    17161 ctaacccaac ctggacgcct acggcaggca ttcgtcagcg ttcgctggag cgtggcatta
    17221 aattaccgcc agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc
    17281 tcgcgcatgg taatggcgaa tacctcattc atggcaccag tgcgccggac agcgttggtt
    17341 tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca
    17401 gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac
    17461 ctaacgggat gcgttatgtt gaagtacatc gaccactatc agcagaagaa cagcaaaacg
    17521 ttcagacaat gccatacaca ctgccagcag gctttacgca atttaaagac aataaggctg
    17581 tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gtttcggtga
    17641 gcagtggagc aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg
    17701 aaccagagca agggaatatg ttacgcgcga cgcagtagtg gtaaatagca gacaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catcttaaat aaagtgagat
    18241 tatatagtga aatccgattt gattttttat tgcttctggt tatcgattaa ataaaaaaag
    18301 cgcccatcag ggcgcttcga tatacaaatt aattcacaaa agcaatatta caggacgtga
    18361 acagatgcgg tgttagtagt gccgctcggt accagtgcac cagaaaccat aactacgacg
    18421 tcacctttgt gtgccagacc gctctgcaga gccagttctt tacccagacg gtagaaatca
    18481 tcagtagaag tgatctcttt aaccagctgc ggcacaacgc ctttgctcag taccaactga
    18541 tgagccgttt tttcgttggt ggtcagtgcc aggatggtgg catccgggaa gtatttacgt
    18601 actgcgcgag cagatttacc gccctgggta gcaaccacga tcagcggagc atccagtttt
    18661 tcagcagttt caacggcacc acggcatacc gcttcggtga tgcgcagttt acggttgtca
    18721 ttgttgaact cgagacggct gttcatcacg cggtcggtac gttcgcagat ggtcgccatg
    18781 atagaaaccg cttccagcgg gtatttacct tttgcggatt caccagacag catcactgcg
    18841 tcagtaccgt cgaggatggc gtttgcaacg tcaccggctt ctgcgcgagt cgggcgtggg
    18901 tttttgatca tggaatccag catctgggtc gcagtgataa cgactttacg tgcacggata
    18961 catttttcga tcatcatctt ctgggcgaag ataacttctt ctaccgggat ttctacaccc
    19021 aggtcgccac gcgcaaccat gatgccgtca gaggcttcga ggatttcgtc gaagttgttg
    19081 aggccttcct ggttttcgat tttggagatg atgtggatgt tttcgccgcc gtgcgctttc
    19141 aggtgctcac ggatttcgat aacgtcagaa cgcttacgaa taaaggaagc agcaacaaag
    19201 tctacgcctt tttcgcaacc aaagatcagg tcctgtttgt ctttttcagc cagtgctggc
    19261 agagcaatgg aaacgccagg caggttcaca cctttgtttt cgcccaggtc accgttgttc
    19321 agcactttac agataacttt gttaccttca atggcggtaa cttccatacc gatcagacca
    19381 tcgtcaacca gtacggtgtt gccaacagac aggtcagtag tgaaaccttc atacgttacc
    19441 gcaaccattt cgctgttgcc gataacagat ttatcagtgg tgaaagtaaa ggtctgacca
    19501 gctttcagag aaacatcgtt accgccttcc agtttcatgg tgcggatttc cggacctttg
    19561 gtatcaagca ggatagcggc ggttttacca gttttgctca tcacgttgcg cagattctga
    19621 atgcgctgac cgtgttctgc atagtcacca tgagagaagt tcagacgcat aacgttcatg
    19681 ccagcgtcca gcattttagc taacatctct tcagattcgg ttttcggtcc gatggtgcaa
    19741 acaattttgg tctttttcat gacagtctta gtctttaagt tgagaaggat gggagaaact
    19801 tgctttctgg gcgcactgcc ggaaagcgaa tccggtatta cgaaagtggt ggtgcctcgc
    19861 tctaaggata ggtgacatcg aaaaagcgtg cagaggaatg tgtgcttgtg tctcagccca
    19921 acgtgagtgt ctgtttttga gtcgttgagt tctacagtcc aaagtgctga aaccattcaa
    19981 gagtcaattg gcgcgcatta tacgctgaaa ttaatccaaa aggaaaacaa aaacagcgtt
    20041 tcaaaaaata tacaacttgt tagacaataa attgttatca aagatttaac gttccaggga
    20101 aaaggttacg caaaaacttc gctgtttgcg caacgtctgg tgccgaagtt gtcagtgtga
    20161 ggataagtga aagttcatcc ggtgtataaa tatatcatcg ctcaattgct acagttaaaa
    20221 taatttatcg cgttatttgt ctgattttgt ttgatttttg tttaattggt tagattgata
    20281 tatgctga
//
LOCUS       DAJTSF010000075        20284 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000075 DAJTSF010000000
VERSION     DAJTSF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.075"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="OO335_002799"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="OO335_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6003123.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="OO335_002800"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="OO335_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6003124.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="OO335_002801"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="OO335_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="OO335_002802"
     CDS             1631..2341
                     /locus_tag="OO335_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6003125.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="OO335_002803"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="OO335_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6003126.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4119)
                     /locus_tag="OO335_002804"
     CDS             complement(3469..4119)
                     /locus_tag="OO335_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6003127.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4282..6480
                     /locus_tag="OO335_002805"
     CDS             4282..6480
                     /locus_tag="OO335_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6003128.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6449..6889)
                     /locus_tag="OO335_002806"
     CDS             complement(6449..6889)
                     /locus_tag="OO335_002806"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6003129.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6886..7449)
                     /gene="tag"
                     /locus_tag="OO335_002807"
     CDS             complement(6886..7449)
                     /gene="tag"
                     /locus_tag="OO335_002807"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6003130.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7607..8305
                     /locus_tag="OO335_002808"
     CDS             7607..8305
                     /locus_tag="OO335_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6003131.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8534..9742
                     /locus_tag="OO335_002809"
     CDS             8534..9742
                     /locus_tag="OO335_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6003132.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10066..11757
                     /gene="eptB"
                     /locus_tag="OO335_002810"
     CDS             10066..11757
                     /gene="eptB"
                     /locus_tag="OO335_002810"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6003133.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11849..11925
                     /locus_tag="OO335_002811"
     tRNA            11849..11925
                     /locus_tag="OO335_002811"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11883..11885,aa:Pro,seq:cgg)
     gene            12669..14276
                     /gene="dppA"
                     /locus_tag="OO335_002812"
     CDS             12669..14276
                     /gene="dppA"
                     /locus_tag="OO335_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR6003134.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14584..15603
                     /gene="dppB"
                     /locus_tag="OO335_002813"
     CDS             14584..15603
                     /gene="dppB"
                     /locus_tag="OO335_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6003135.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15613..16515
                     /gene="dppC"
                     /locus_tag="OO335_002814"
     CDS             15613..16515
                     /gene="dppC"
                     /locus_tag="OO335_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6003136.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16526..17509
                     /gene="dppD"
                     /locus_tag="OO335_002815"
     CDS             16526..17509
                     /gene="dppD"
                     /locus_tag="OO335_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6003137.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17506..18510
                     /gene="dppF"
                     /locus_tag="OO335_002816"
     CDS             17506..18510
                     /gene="dppF"
                     /locus_tag="OO335_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6003138.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18540..19811)
                     /locus_tag="OO335_002817"
     CDS             complement(18540..19811)
                     /locus_tag="OO335_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6003139.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4866 a         5022 c         5333 g         5063 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggcaggc
     4561 ggttataccg ggcatctggg ggattattcg accggtgcgg cacaggcgat catgccgtat
     4621 gtcgtaggtg gcagcgaagt ttatcaacag caaaccagtt ggccgctggt gctggaacat
     4681 agcgatgtcg tggtgttgtg gagtgctaac ccactcaata cgctgaaaat tgcgtggaat
     4741 gcatccgatg agcaggggct ttcttacttt tccgcactgc gtgacagtgg gaaaaagctg
     4801 atctgcattg atccaatgcg atcggaaacc gtcgatttct ttggcgataa aatggagtgg
     4861 gtggcaccgc acatgggcac cgatgttgcg ctgatgctgg ggatcgccca tacgctggtg
     4921 gaaaatggtt ggcacgacga agcgtttctg gcgcgttgca ccacaggtta tgccgtcttc
     4981 gcctcttatt tgctgggcga gagtgacgga atagcgaaaa acgccgaatg ggcggcagag
     5041 atttgtggtg ttggcgcagc gaaaatccgc gagctggcgg ctattttcca ccaaaatacc
     5101 accatgctga tggcaggctg gggaatgcag cgccaacagt ttggtgagca aaaacactgg
     5161 atgatcgtca cgctggcagc aatgttgggg caaatcggca cacccggcgg cggttttggt
     5221 ctttcttacc attttgccaa tggtggtgac cccacgcggc gttctgcggt gctctcttcc
     5281 atgcagggca gcttgccggg tggctgcgat gcggtggata aaatccctgt tgcccgcatt
     5341 gttgaagcac tggaaaaccc tggtggcgca tatcaacaca acggtatgaa ccgacatttc
     5401 ccggatattc gttttatctg gtgggcgggc ggtgccaact ttactcatca tcaggatacc
     5461 aatcgtctga tccgtgcctg gcaaaaaccg gagctggtgg tgatctctga atgcttctgg
     5521 acggcggcgg cgaaacacgc ggatattgtt ctgcctgcga ctacctcttt tgagcgtaat
     5581 gatctcacca tgaccggtga ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg
     5641 ccgccacgct atgaagcgcg taatgatttt gatgtttttg ccgagttgag tgaacgttgg
     5701 gagaagggcg gctatgcgcg gtttacggaa ggaaaaagtc agctgcaatg gctggaaacg
     5761 ttttataacg ttgcccggca gcgcggggca agccagcagg ttgaattgcc gccatttgct
     5821 gagttctggc aagccaacca gttaattgag atgccggaaa acccggacag cgagcggttt
     5881 attcgcttcg ctgatttttg ccgcgatccg ctggcgcatc cgttaaaaac cgccagcggc
     5941 aagattgaaa tcttctcaca gcgtattgcc gattacggtt acccggattg ccctgggcat
     6001 ccaatgtggc tggagccgga cgaatggcag ggcaatgccg aaccagaaca gttgcaggta
     6061 ctttctgccc atccggcgca ccgcctgcac agccagctga attacagttc tctgcgcgaa
     6121 ttgtacgcgg tagcaaaccg tgagcctgtc accattcatc ctgacgatgc tcaggcgcgc
     6181 ggcataaccg aaggggatat ggtgcgggtg tggaactccc gtgggcagat ccttgccgga
     6241 gcggtcatta gcgagggaat taaacctggc gtgatttgca ttcacgaagg ggcatggccg
     6301 gatctggatt taaccgctga cggtatttgt aaaaacggcg cagtgaacgt gctgaccaaa
     6361 gatctcccca gctcgcggct ggggaatggc tgtgcgggta atacgacgct ggcatggctg
     6421 gaaaaataca acggtccgga actgacactt acagcgtttg aaccaccggc cagctcataa
     6481 tccaggtggg tagttgggtt tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg
     6541 cacggtaaaa atctatcgcc ggttgatttt tttgataaac ctccagcatc agatggggat
     6601 agcgctgctg cacatactgc atcagcgcct taccaatacc gcgtctgacg gccttcggtg
     6661 cgacaaacat tgctgccaga aatcggcctt ccataatgct gacaaaaccg agaagcttac
     6721 cgtcctcttc ccagacccag ttttgcgcgt tggcaagata ggcatcccgc accagcggaa
     6781 tgcagtcacg ccagtaattc gcttttataa agggatgccc ccaggttgta ctttccagcc
     6841 acagttcgag gatcgcgggg agttctgaac gttgcgcttc ccgaatcatg gtttatttcc
     6901 cagatagcaa cagcagccaa ccacatgatc attcaccagc ccacatgcct gcataaagga
     6961 gtaacagatt gtggtgccga caaacttaaa accacgtttt ttcagtgcct tagatagggc
     7021 gtcggaggcg gacgtagatg tgggaatttc gctcaacgtt gtggcttgtg tcacctgtgg
     7081 ctgatgattt acaaacgacc agacaaagtc gggaaacggt tcgccgttct gttccatttg
     7141 caggtacgcc cgcgcattac caataattgc ctgaattttc cctcgatggc ggataatccc
     7201 ggcgtcctgt accagtcttt cgacatcctc ttcctgcatt gctgcgacct tcaccggatc
     7261 gaactgatga aagtaggcgc gatagttttc gcgttttttg aggacggtga tccacgataa
     7321 cccagcctgc tgcccttcaa agcagatcat ttcgaacagt tttttactgt cagtttcagg
     7381 cacgccccac tcattatcat ggtaggcaat ataaagcggg ccctgactca cccagccgca
     7441 acgttccata ctttccctcg ctatgtaccc gatcgtaaaa atttcagccg cttttcttga
     7501 cgcatcggtg aaaaagtcaa tatttaatac agggctacta tgcccgatat ccttcataca
     7561 atgacaataa tattgccgaa ttcggctctt ccctggagtc gtgttaatga tcataaaaaa
     7621 aagcggtggt cgctggcagc taagcctgct ggcgagcgtg gtaatcagtg ccttttttct
     7681 caacacagct tacgcctggc aacaagaata tatcgttgat acgcaacccg gacattccac
     7741 agagcgttac acctgggata gtgatcatca acctgattac aacgatattt tgtcgcaacg
     7801 tattcaaagt agccaaaggg cgctgggact ggaagtcaat ctggcggaag aaactcctgt
     7861 ggatgtgacc agcagtatga gtatgggctg gaattttcct ttatatgaac aggttacaac
     7921 cggcccggtc gcggcattac attacgatgg cacaaccacc tcgatgtata acgagtttgg
     7981 cgacagtact accacgctga ccgatccgtt atggcatgcc agcgtgagta gcttaggctg
     8041 gcgtgttgac tcccggcttg gcgatctccg accctgggcg caaatcagct ataaccagca
     8101 atttggcgag aatatctgga aggcgcaatc aggcctgagc cggatgacgg cgacaaacca
     8161 gaacggcaac tggctggatg tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc
     8221 cgcctatgct gcgctaactc aggcagaaaa taccactaat aatagcgact atctgtatac
     8281 gatgggggtt agcgccagat tttaacgtaa cagtcacaat tgaaaccatt gaataacaac
     8341 agttgtggcg atagtgggtg ctaacttacc aaataataaa tttggtgaat aattgtcgcg
     8401 tcattcattc ctgaactaag gcatttcatt ccgttctgat ggcatttcat gccgtttttc
     8461 cccaagcata aaatgcactt cgttatggtt gtcggcagag atttttcctt tttattactg
     8521 caggaatact gccatgacac cttcaaatta tcagcgtacc cgctggctga cactcatcgg
     8581 tactatcatt acccagtttg cgctggggtc ggtttatacc cggagcctgt ttaatggcgc
     8641 gctttctgcc aagctggatg cgccggtaag ccaggtcgct ttctctttcg gcttgttaag
     8701 tctggggctg gcaatttcgt cttctgttgc gggcaaattg caggaacgtt ttggcgttaa
     8761 acgcgtcacc gtggcttccg gcattttgct gggattaggc ttcttcctga ccgcgcattc
     8821 caacaacctg atgatgctgt ggttaagcgc cggtgtgctg gtcggtctgg cggatggcgc
     8881 gggttacctg ctgacgctct ctaactgcgt gaagtggttc ccggagcgta aaggtctgat
     8941 ctccgcgttc gctatcggtt cttatggtct gggtagcctg ggtttcaaat ttatcgacac
     9001 gcagctgctc gaaacggtgg gtctggaaaa aacctttgtg atttggggag cgattgcgct
     9061 ggtgatgatt gttttcggcg caacgttaat gaaagacgca ccaaaacagg aagtgaaaac
     9121 cagcaatggt gtggtggaga aagattacac gctggcagag tcgatgcgta aaccgcagta
     9181 ctggatgtta gcggtaatgt tcctgactgc ctgcatgagc ggcctgtacg tgattggggt
     9241 agcgaaagat atcgcccaaa gtctggcaca ccttgatgtg gtttccgcag ccaatgcagt
     9301 aacggttatt tccatcgcta acctttcagg tcgtctggtg ctgggcattc tgtctgacaa
     9361 aattgcccgt atccgtgtta tcaccattgg tcaggtgatt tcgctggtgg gtatggcggc
     9421 cctgctgttt gcaccattaa atgcagtgac gttctttgca gcgattgcct gcgtggcatt
     9481 taactttggc ggcactatta ccgtctttcc atcactggtc agtgagttct tcggcctcaa
     9541 taacctggcg aaaaactacg gtgtgattta cctcggtttc ggtatcggta gcatttttgg
     9601 ttcgattatc gcctcactgt ttggcggttt ctatgtgacc ttctacgtga ttttcgccct
     9661 gctgattctg tcattggcgc tttctacgac gattcgtcag ccagagcaga aaatgttgcg
     9721 tgaggcgcat ggctcccttt aatgctcagc cgcgagctac tgggcttcag cttccgtgtg
     9781 catcaaacgc atcaacgaaa tgtataaaca atgactcgta aaatttgggc cagttaactg
     9841 gcccttttta ctgctttttg taacgctaaa gtctcttttc aaacttgcat ttttgtaaat
     9901 ttgtgcttca tgcacactct ttccccacac tttttcgctt tgctgtggtc tacttattcg
     9961 cgcgtgtaga ttttacttat ctgactacct ccgcactttt cccctgccgg gcctgaaaag
    10021 ccgctaagca gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa
    10081 tcgattacac agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat
    10141 ggcgcggttt tttatcgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc
    10201 gtttctgctg ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt
    10261 ctatcgctgt ttggccgccg cagctggcgt attctggcgt cgctggtggt gctcttttcc
    10321 gcaggtgcca gctattacat gaccttcctt aatgtggtca ttggttatgg catcatcgct
    10381 tccgtcatga ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc
    10441 tggttaatcg ccgttagtgc attgcctctt atccttatct ggaataaccg ctgtcgctac
    10501 accttgctcc gacaactgcg aaccccaggg cagcgtattc gcagcctggt ggtcgtcgta
    10561 ctggcgggta ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg
    10621 gagagggcga ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg
    10681 ccatcaaact ggctttctgc gttggggctg tatgcctggg cgcgggtcga tgaatcttcc
    10741 gataataatt cattgcttaa tccggcgaag aagtttacat accaggctcc acaaaacgtt
    10801 gatgatactt atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggtatt
    10861 ttcggctatg agcgtaatac cacgccgaaa ctggcccagg agaaaaatct ggcggcgttc
    10921 cgtggttact cctgtgatac cgcaaccaaa ctctcactgc gttgcatgtt tgtacgtcag
    10981 gggggcgcgg aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggttctg
    11041 aagcagttag gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc
    11101 aacacgatgg cagacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaatcgt
    11161 ggcaagccgg tagatgatat gttgctggta gacgaaatgc agcaatcgct agggcgcaac
    11221 ccggatggta agcatctgat cattctgcat accaaaggtt cgcattttaa ctacacccag
    11281 cgttatccgc gtagcttcgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt
    11341 accaaagcgc agatgatcaa ctcctatgac aactcggtga cctatgtgga tcactttatc
    11401 tccagcgtga ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac
    11461 ggtgagtcaa ttaatgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg
    11521 gagcagttcc gcgtaccgat gatggtctgg atgtcagata aatatctgga aaatccggcc
    11581 aatgcgcagg cgtttgcgca gctgaaaaaa gaagccgaca tgaaagtgcc acgccgtcac
    11641 gtagagctgt acgacaccat catgggttgt cttggctata cttcaccgga tggtggaatt
    11701 aacgaaaaca acaactggtg tcacatcccg cagacaaaag tggcagcggc taactaagcg
    11761 gcctgctgac tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt
    11821 atctgcgcag taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg
    11881 ttcgggacga aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg
    11941 ctcaacgagc aggttttttt gcatttgtag ctcatgagga tgagaacctc cgggggcagg
    12001 gaggctcgac tcgacgtagg cctgataaga cgcgccagcg tcgcatcagg caacggcacc
    12061 gcacttattg tcggatgcgg cgcgaccgcc ttattcgacc aacacaccac acattttcct
    12121 gccaactttg cgccccatat ctcactatcc attaacggat ttgtgacaga aaccattgtc
    12181 tttttttata tttgcttaaa tcttttttgc gttacttatg gctggcgctg gcattttccg
    12241 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
    12301 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
    12361 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
    12421 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
    12481 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
    12541 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
    12601 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
    12661 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
    12721 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
    12781 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
    12841 tcccgcttta taaccgcctg gttgaattta aaatcggcac caccgaagtg atcccgggcc
    12901 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
    12961 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg cgaaatgaac gccgatgacg
    13021 tggtgttctc gttcgatcgt cagaaaaacg cgcaaaaccc gtaccataaa gtttctggcg
    13081 gcagctacga atacttcgaa ggtatgggct tgccggagct gatcagcgaa gtgaaaaagg
    13141 tggacgataa caccgttcag tttgtgctga ctcgaccgga agcgccgttc ctcgctgacc
    13201 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgacgcgatg atgaaagccg
    13261 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
    13321 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
    13381 agatcgatac gctggtcttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
    13441 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
    13501 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcgtata
    13561 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca agcactaacc tacgcggtga
    13621 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
    13681 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
    13741 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
    13801 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
    13861 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    13921 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    13981 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    14041 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    14101 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcagg
    14161 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    14221 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    14281 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    14341 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    14401 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    14461 cctctgcgtg cagcgccatc cggcagcaat acttcattct ctgtccgaca agggcgggaa
    14521 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg agtcactata gagaatccgg
    14581 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    14641 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    14701 ggcgaacgtg ggatctcccc tgagcgtcac gtgcagctgc tggctgaact cggcttagat
    14761 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    14821 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    14881 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    14941 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    15001 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    15061 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    15121 ccgttaaccg gttttatgct aatcgacacc gccatctggg gtgaagacgg caactttatc
    15181 gatgccgtcg cccatatgat cttgcccgcc attgtgctgg gtactattcc gctagcggtc
    15241 attgtgcgta tgacacgctc ctcgatgctg gaagtgctgg gcgaggatta catccgcacc
    15301 gcgcgcgcca aagggctaac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    15361 atgctgccgg tagtgaccgt tatcggcctc caggtgggaa cattgctggc gggggcgatt
    15421 ctgaccgaaa ccatcttctc gtggcccggt ctggggcgct ggttgattga cgcactgcaa
    15481 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    15541 gtcaacttgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    15601 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    15661 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt cgtcgggctg
    15721 gtttacgtcg ccatcgtgct gttcatcgcg atctttgcca actggattgc gccttataac
    15781 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagt
    15841 atggcacact tgctgggcac cgatgacgtc ggtcgtgatg tgctgtcgcg gctgatgtac
    15901 ggcgcgcgtt tatcgctgct ggttggctgt ctggtggtgg tgttgtcgct gattatgggc
    15961 gttattctcg gcctgatcgc cggttacttc ggcggcctgg ttgataacat tatcatgcgc
    16021 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    16081 ttcggcccgt cgattggtaa cgccgcgctg gcgctgacct tcgttgcctt gccgcactat
    16141 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt caccgcctct
    16201 cgcgtggcag gtgccggggc gatgcgccag atgtttatta acatcttccc gaactgcctt
    16261 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggctgct
    16321 cttggtttcc tcggcatggg ggcacagccg ccaacgcctg agtggggcac catgctctcc
    16381 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttccctgg tctggcgatc
    16441 ctgttgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    16501 aaactaaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    16561 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    16621 gggcgaagtg gtcgggattg tgggtgagtc cggctccggt aagtcggtca gttcactggc
    16681 gattatgggg ctgattgatt atccgggccg cgtaatggca gaaaaactgg agtttaacgg
    16741 ccaggatttg cagcgtatct cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    16801 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    16861 tatggaagcg attaaagtgc atcagggcgg caacaaaagt acccgccgtc agcgggcgat
    16921 tgacctgctg aatcaggtcg gtattcccga tccggcttcg cgtctggatg tttacccgca
    16981 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    17041 aaaactgctg attgccgatg aaccgaccac cgcgctggac gtgaccattc aggcgcaaat
    17101 catcgaacta ctgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    17161 tgacctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggtcaggt
    17221 agtagaaacg ggcgatgcac acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    17281 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    17341 tgttgttccc ggcaagtatg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    17401 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg atgggcgtca
    17461 gtccaaatgc cattacccac ttgatgatgc cgggaggccc acactatgag tacgcaagag
    17521 gccacctcgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    17581 aaaggcatgt tcgtgccgga acgtctggtg aaggcgctgg acggcgtttc gtttaacctt
    17641 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    17701 cggttgctga cgatgatcga aacgcccacc ggcggcgagc tgtattatca ggggcaggat
    17761 ctgctcaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    17821 cagaatccct acggttcgct gaatccgcgt aagaaagttg ggcaaattct cgaagagccg
    17881 ctgcttatta acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    17941 gcaaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcacatgtt ttccggcgga
    18001 caacgtcagc gtatcgctat tgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    18061 gatgagccgg tttctgcgct ggacgtctca gtgcgtgcgc aggtgctgaa tctgatgatg
    18121 gatttgcagc aggagttggg gctttcttat gtctttatct cccacgacct gtcggttgtg
    18181 gagcacattg ctgatgaagt gatggtgatg tacctaggcc gctgcgtgga gaaaggaacg
    18241 aaggaccaaa tcttcaataa cccgcgccat ccgtacaccc aggcgctgct ctccgcgacg
    18301 ccacgcctga acccggacga tcgtcgtgaa cgcatcaaac tcaccggtga actgccaagc
    18361 ccgctgaatc ctccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    18421 tgcacccagt tgcagccgca gctaaaagac tacggaggtc aactggtagc ttgttttgct
    18481 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    18541 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtaaag cagaatcagc
    18601 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    18661 atgaggcacg acacaatgcc atataacggg cttcccaact gaaagaacac cagcactgat
    18721 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    18781 agcatgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    18841 cctttaatag cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    18901 atattcagca ccgtagaggt gatatgaatg atatgtccag ggatcacctg cgcggccagt
    18961 gccagtgccg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    19021 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    19081 gtacgcagcg cgagccgggt tgccagtact ttatctgctt cccgtttacg ataggcaata
    19141 ttcattgggt taagtacctg aataaatacc gcagaaaaga agcaaaatgg aatggtaagc
    19201 aggacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    19261 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    19321 ccagaaatct taaacagtaa tcgttcacca ccagacgcaa tcgctatcag tacggcgaaa
    19381 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    19441 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    19501 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    19561 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    19621 actacccagg tgacaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    19681 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    19741 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    19801 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    19861 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    19921 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    19981 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    20041 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agcaatcgta gaatcagcgt
    20101 caagccggaa ggattccggt gaggcgcaat gttgcggggg cttaatccct ggtggcattg
    20161 gttgctggag agagaaaacc cccgcacgtt gcaggtatgt acctgacaac ataacggggg
    20221 ctaatcttga acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc
    20281 ggct
//
LOCUS       DAJTSF010000076        19769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000076 DAJTSF010000000
VERSION     DAJTSF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.076"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(6..82)
                     /locus_tag="OO335_002818"
     tRNA            complement(6..82)
                     /locus_tag="OO335_002818"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46..48),aa:Met,seq:cat)
     gene            291..1388
                     /gene="mltA"
                     /locus_tag="OO335_002819"
     CDS             291..1388
                     /gene="mltA"
                     /locus_tag="OO335_002819"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6003140.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1626..2432
                     /gene="tcdA"
                     /locus_tag="OO335_002820"
     CDS             1626..2432
                     /gene="tcdA"
                     /locus_tag="OO335_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6003141.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2483..2926)
                     /gene="csdE"
                     /locus_tag="OO335_002821"
     CDS             complement(2483..2926)
                     /gene="csdE"
                     /locus_tag="OO335_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6003142.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2926..4131)
                     /gene="csdA"
                     /locus_tag="OO335_002822"
     CDS             complement(2926..4131)
                     /gene="csdA"
                     /locus_tag="OO335_002822"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6003143.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4323..4550
                     /locus_tag="OO335_002823"
     CDS             4323..4550
                     /locus_tag="OO335_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6003144.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4901..5818
                     /gene="gcvA"
                     /locus_tag="OO335_002824"
     CDS             4901..5818
                     /gene="gcvA"
                     /locus_tag="OO335_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6003145.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5837..6232
                     /locus_tag="OO335_002825"
     CDS             5837..6232
                     /locus_tag="OO335_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6003146.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6225..7325
                     /gene="rlmM"
                     /locus_tag="OO335_002826"
     CDS             6225..7325
                     /gene="rlmM"
                     /locus_tag="OO335_002826"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6003147.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7369..8100)
                     /gene="fucR"
                     /locus_tag="OO335_002827"
     CDS             complement(7369..8100)
                     /gene="fucR"
                     /locus_tag="OO335_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6003148.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8158..8580)
                     /gene="fucU"
                     /locus_tag="OO335_002828"
     CDS             complement(8158..8580)
                     /gene="fucU"
                     /locus_tag="OO335_002828"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6003149.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8582..10001)
                     /gene="fucK"
                     /locus_tag="OO335_002829"
                     /pseudo
     CDS             complement(8582..10001)
                     /gene="fucK"
                     /locus_tag="OO335_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(10110..11885)
                     /gene="fucI"
                     /locus_tag="OO335_002830"
     CDS             complement(10110..11885)
                     /gene="fucI"
                     /locus_tag="OO335_002830"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR6003150.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11918..13234)
                     /gene="fucP"
                     /locus_tag="OO335_002831"
     CDS             complement(11918..13234)
                     /gene="fucP"
                     /locus_tag="OO335_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6003151.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13781..14428
                     /gene="fucA"
                     /locus_tag="OO335_002832"
     CDS             13781..14428
                     /gene="fucA"
                     /locus_tag="OO335_002832"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6003152.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14456..15604
                     /gene="fucO"
                     /locus_tag="OO335_002833"
     CDS             14456..15604
                     /gene="fucO"
                     /locus_tag="OO335_002833"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR6003153.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15657..16412)
                     /gene="xni"
                     /locus_tag="OO335_002834"
     CDS             complement(15657..16412)
                     /gene="xni"
                     /locus_tag="OO335_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6003154.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16523..17890)
                     /gene="sdaB"
                     /locus_tag="OO335_002835"
     CDS             complement(16523..17890)
                     /gene="sdaB"
                     /locus_tag="OO335_002835"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6003155.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17948..19237)
                     /gene="sdaC"
                     /locus_tag="OO335_002836"
     CDS             complement(17948..19237)
                     /gene="sdaC"
                     /locus_tag="OO335_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6003156.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4874 a         4843 c         5221 g         4831 t
ORIGIN      
        1 ttgattggtt gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc
       61 taccaggctg ctccaccccg cgtccgtgga tgcgcactat actccgctcg cgttttcatg
      121 caaccttttt tttaccccga tcacggattt agcatgattt tgaacaaaaa caaaccacta
      181 aagcctattt tttgtgcaaa atttgcgtca gggagaggct gctcatttca ttaaacgcgg
      241 cggtttgtta tcttcgttgc gccttatttt ttaacctgaa gaagagaaca atgaaaggac
      301 gttgggtaaa gtaccttctt atgggcacgg ttgtggcaat gcttgccgcc tgctcttcca
      361 aaccaaccga tcgcggacag caatataaag acgggaaatt tacccagcct ttctctctgg
      421 tgaaccagcc agatgccgtt ggcgcgccga ttaacgccgg tgattttgcc gagcaaatta
      481 accatatccg taattcgtca ccgcgtctgt atggcaacca gagtaatgtt tataacgcgg
      541 tgcaagagtg gctgcgcgca ggcggtgata cccgcaatat gcgccagttc ggcattgatg
      601 cctggcagat ggaaggtgcc gacaactatg gtaacgtgca gtttacaggc tattacacgc
      661 cggtaattca ggcgcgccat acccgccagg gcgagttcca gtatcctatt taccgtatgc
      721 cgccaaaacg tggtcgtctg ccgtctcgtg cggagatcta cgccggggca ttgggcgata
      781 aatacatcct cgcttacagc aactctctga tggataactt cattatggat gtgcagggta
      841 gtgggtatat cgactttggt gatggcagtc cgcttaactt tttcagctat gcagggaaaa
      901 acggtcatgc ctatcgcagc attggtaagg tgctgatcga ccgtggcgaa gtgaaaaaag
      961 aagatatgtc gatgcaggcg attcgtcatt ggggcgaaac acacagtgaa gccgaggttc
     1021 gcgagctgct ggaacagaac ccgtctttcg tcttctttaa accgcaatct tttgcaccgg
     1081 tgaaaggggc aagtgcggtg ccgctggttg gccgtgcttc tgttgcctcc gatcgttcaa
     1141 ttattccgcc aggtactacc ttgctggcag aagtgccgct gctggataat aacggcaaat
     1201 ttaacggtca gtacgaattg cgtctgatgg tggcgttgga tgtcgggggc gcaatcaaag
     1261 gccaacactt cgatatctat caagggatcg ggccggaagc cggacaccgc gcaggttggt
     1321 acaaccacta tggacgggtt tgggtgctga aaacggctcc gggcgcaggt aacgtcttta
     1381 gcggctgatg tgatattctg tatgccgttt ttacggatat gacaggggag gggtaacctc
     1441 acatcctgat tattgactaa gtgcgcgttg ttcataccgg atgcggcgtg aacgccttat
     1501 ccggcctaca aatcatgcaa atccaaaata ttgcaggagt tatgtaggcc tgataagcgt
     1561 agcgcatcag gcatttatgc gtttagggtg agggataacc tcgccctttt taattctgag
     1621 gtgctatgtc tgtggtaatt agtgatgcat ggcgtcagcg ttttggtggc acagcgcgtc
     1681 tgtatggtga aaaagcgttg caactgtttg ctgacgcgca tatttgtgtg gttggtatcg
     1741 gtggtgtcgg ttcctgggcg gcggaagcgc tggcgcgcac ggggattggc gcaatcacgc
     1801 ttatcgatat ggatgatgtg tgcgtcacca ataccaatcg gcaaattcac gctctgcgcg
     1861 ataacgtcgg gctggcaaaa gcggaagtca tggcggagcg cattcgccag attaacccgg
     1921 agtgccgtgt aacggtggtg gatgatttcg tgacgccgga taacgtagcg caatatatga
     1981 gtgccggtta ttcgtacgtg attgatgcca ttgatagtgt acggcccaaa gcggcgctga
     2041 ttgcttattg tcggcgcaat aaaatcccgc tggtcacgac cggtggcgcg ggtgggcaga
     2101 ttgacccgac gcagattcag gttaccgatc tggcgaaaac gattcaggat ccgctggcgg
     2161 cgaagttgcg cgagcgcctg aaaagcgatt ttggcgtagt gaaaaacagt aaaggtaagc
     2221 tcggcgtgga ttgcgtgttt tctactgaag cgctggtgta cccgcagtca gacggtacgg
     2281 tgtgtgcgat gaaagccacg gcagaagggc cgaagcggat ggattgtgca tctggatttg
     2341 gcgcggcaac gatggtgacc gccacctttg ggtttgttgc ggtttctcat gcgctgaaga
     2401 agatgatggc gaaagcggcg cgtcagggtt aaacaatcag gtcggatgag acgcgaacgt
     2461 cttatccgac ccggttctgt gcttaaacct gcttcgtcgc agcgataatc gcctcgctta
     2521 acgcatttaa cccctggctg cgtgaggcgc taagctgcgc acgtaatccc agctcatcaa
     2581 acaatgccag cggcgactgt gcctgtaatt cggcggcggt tttcccctca acggcagtca
     2641 acaacaccgc caacaggccg cgcacaatgc gcccttcgct gttgccaaag aagtgcattt
     2701 tgccgttttc agccacggta tatcccagcc agacgcggtt ttcgcatccg gcaatctctt
     2761 tagcctgcgc ttttaactcg tctggcaaag ccggaagctg tttccccagc atgatcaact
     2821 ggcgatattt atcttcccat tgcgacaacg gtgcgaaggt attgcgtaac gtttctgcgg
     2881 ttacggttgt gccgaacgga tgtccggcga attgcgggtt tgtcattaat ccaccaataa
     2941 ttccagcgcg cggtcaacgg cattcaccag cgcatccaca tcactctttg tattatatgg
     3001 cgcaaatgag gcgcgcagtg tgccggttac gcctaattct gccagtagcg gctgagcgca
     3061 atgttgcccg gcccgcaagg caataccgta ctccgccagt agcgtcacca tatcgctgtg
     3121 atgaacgcca gcaaaatcaa aggccagcag gctggaatcc tggcagcgga atgaacgaaa
     3181 gcctggacgt ttcgccagcg cttcttctgc aagcgttgct aagctacggc tccagctttc
     3241 ggcctggttg atatcgtaat ctgccagcca ttccagcgcc gcgcttaatc ctatgacgcc
     3301 agcgacattt ggcgttcctg cttccagttt ccacggcgca gattgagtgg tgaaaccgtc
     3361 aaaactcact tcgtgaatca ttttgccgcc acccagccag ggcgacatcg cttccagcag
     3421 ttctgatttg ccatacagca cgccgatacc cgtcgggcca tacagtttgt gaccagagaa
     3481 agcatagaaa tcgatatcca gttgctgaac atccgcgggg aaatgcaccg tcccctgagc
     3541 accatcaacc atcaccacca tcccggctga atgagcaaag gtaatcgctc gcgccagatc
     3601 cgggcaaccg ccagtcacgt tcgacatctg acccaacgcc agaatccgac tacggggagt
     3661 aatcagctct ggcaacaaat cgacatccgg cagtcgctgc gcattaagcg gcaatttcac
     3721 cactttggct ctagtttgtt gggcgaccat cagccagggg acgaggttag cgtggtgttc
     3781 tgccacgctg acaataatct catcgcccgg ttgcagacgc ggacgcgcat agcattgtgc
     3841 caccatgttg atggattcag tggtgccgcg cgtccagacg atagttttat catccggtgc
     3901 attcagtaat tgcgccactt tctctcgtgc agcttcataa cgcgcggtca ggcgttgggc
     3961 ttcggcaaac tggctgcgat ggacgtttcc ggcgctcagg ctgtaaaact gtcgagtggc
     4021 ttcaaccacg gcttcaggtt taagcgcggt cgcggcgctg tcgagataga cgcccgcatc
     4081 ctgtagtgcg ggaaactggg cgcgaaactg cgcgggatta aaaacgttca tggtactcct
     4141 cggcttgatg caccgatcgt gacgcaaaat tcactttgat acaaggagag tgctaaccat
     4201 cagaattatc tggatatcct ggcgaagccg cgtaagtaac atatgctgaa tagtgttagg
     4261 cgttaatttt agcctgttaa tcaatactat taagatttat taatcgtttt aaggagaaga
     4321 tgatgaaaaa gactgccgca attatttctg cctgtatgct gacttttgcc ctgagcgcct
     4381 gttccggttc gaactatgtg atgcacacca atgacggacg taccatcgtc tctgacggca
     4441 aaccacagac tgataacgat atcggtatga tttcgtataa agacgctaat ggcaacaaac
     4501 agcagatcaa ccgtactgac gtgaaagaga tggtcgaact ggaacagtaa ttcgcgatcg
     4561 caaggtaaaa aaaagcaccg caattaggcg gtgctacatt aatcactatg gacagacagg
     4621 gtaaatgtac aggaagtgaa aaaaggtagc tttgctacca tggtctgaat cgcagaccaa
     4681 ttgcaaacac aacaacacaa catcacaacc gtaagccaaa agttcaccag aatacgcatt
     4741 ccgataaaac ttttcgttcc ggctcaggaa gtgccgccac tataggtatt tgctggtaga
     4801 agctcaacgg acaatttata atggctcaga ttaaaaaaac taataggtta cacagtgtga
     4861 tctaattgtt aaattcattt aacatcaaag tttaaaagcc atgtctaaac gattaccacc
     4921 gctaaatgcc ttacgagttt ttgatgccgc agcacgccat ttaagtttta ctcgcgcagc
     4981 agaagagctt tttgtgaccc aggccgcagt aagtcatcaa atcaagtctc ttgaggattt
     5041 tttggggcta aaactgttcc gccgccgtaa tcgttcactc ctgctgaccg aggaagggca
     5101 aagctatttc ctcgatatca aagagatatt ttcgcaatta accgaagcga cgcgtaaact
     5161 ccaggcccgt agcgccaagg gggcgttgac ggtcagttta ctccccagtt tcgccattca
     5221 ttggttggtt ccgcgacttt ccagctttaa ttcagcttat ccgggaattg acgttcgaat
     5281 ccaggcggtt gatcgtcagg aagataagct ggcggatgat gttgatgtgg cgatatttta
     5341 tggtcggggc aactggccgg ggctacgggt ggaaaaactg tacgccgaat atttattgcc
     5401 ggtgtgttcg ccgctactgc tgacaggcga aaaacccttg aagaccccgg aagatctggc
     5461 taaacatacg ttattacatg atgcgtcacg ccgtgactgg cagacatata cccgacagtt
     5521 ggggtttaat catatcaacg ttcagcaagg gccaattttt agtcatagcg ccatggtgct
     5581 gcaagcggct attcacgggc agggagtggc gctggcaaat aacgtgatgg cgcaatctga
     5641 aatcgaggcc ggacgtcttg tttgcccgtt taatgatgtt ctggtcagta aaaatgcttt
     5701 ttatctggtt tgtcatgaca gccaggcaga actgggtaaa atagccgcct ttcgccaatg
     5761 gatcctggcg aaagccgctg ctgaacaaga aaaattccgc tttcgttatg aacaataatt
     5821 tacgtagggt acgaccatga ccagccgttt tatgctgatt ttcgccgcca ttagcggctt
     5881 catttttgtg gcactgggcg cttttggtgc gcatgtgtta agtaaaacca tgggggccgt
     5941 tgagatgggc tggatccaaa ccggtctcga ataccaggcg tttcatacgc tggcgatctt
     6001 aggtctggcg gtggcaatgc agcgtcgcat cagtatctgg ttttactgga gtagcgtttt
     6061 cctcgcgtta ggcacggtgc tgttcagcgg cagcctttat tgcctggcgc tgtcccatct
     6121 gcgtttgtgg gcgtttgtca caccggttgg cggcgtgagc ttccttgcgg gctgggcgtt
     6181 aatgttagtt ggtgctatcc gtttaaagcg caagggcgta agtcatgaat aaggttgtat
     6241 tgctgtgccg tccgggcttt gaaaaagagt gcgccgcaga aattaccgat aaagccggcc
     6301 agcgggaaat tttcggtttt gcccgcgtga aagagaatgc gggttatgtc atttatgaat
     6361 gttatcaacc tgatgatggc gataagttaa tccgtgagct gccgttcagt tcattaattt
     6421 ttgcccgcca gtggtttgtg gtgggggaac tcctgcagca tttgccgcca gaagatcgta
     6481 ttacccccat tgtcggcatg ttacagggtg tagtagagaa gggcggtgaa ctgcgtgttg
     6541 aagttgccga taccaacgaa agcaaagagt tgctgaaatt ctgccgtaag ttcactgttc
     6601 cgctacgcgc tgccttgcgc gatgctgggg tgctggcgaa ctatgaaacg ccgaagcgtc
     6661 cggttgtgca tgtattcttt attgcaccag gatgctgcta taccggttac tcatacagca
     6721 acaataattc gccgttctat atgggcattc cgcgcctgaa atttccggca gaggcgccga
     6781 gtcgttccac gctcaaactg gaagaggcat ttcatgtgtt tattcctgcg gatgagtggg
     6841 atgaacgcct ggcgaacggg atgtgggcgg tggatttagg cgcttgccct ggcggctgga
     6901 cctaccaact ggtgaagcgc aacatgtggg tttattccgt cgacaacggc ccgatggcgc
     6961 aaagtctgat ggataccggg caggtgacgt ggctgcggga agacggtttc aaattccgtc
     7021 cgacacgcag caatatctcc tggatggtat gcgatatggt tgaaaaaccg gctaaagttg
     7081 cggcattgat ggcgcagtgg ctggttaatg gctggtgccg tgaaaccatt ttcaatctca
     7141 aactgccgat gaaaaaacgc tacgaagaag tatcacacaa tctggcgtat attcaggcac
     7201 agcttgatga acatggcata aatgctcaga ttcaggcgcg gcagttgtat cacgatcgcg
     7261 aagaagtgac ggtgcacgtc cgccgcatct gggctgcggt gggtggtcgt cgcgacgagc
     7321 gataacaggc aaggccgggg cgtcgtctcc ggcctgctac cctttccgtc aggctgttac
     7381 caaagaagtt gcaacctggc gctcatcaga aataatgtgc gttacctcat ccagatgccc
     7441 gatgcgggct tcccctgaac gattaaattt acttttatca atcaataaca acgattgcgc
     7501 ggcacgtttt aatagcatcg atttgtaatc agcgttgatc gcattggagt cccacagtgc
     7561 gccgctgcta tcgatccctt cacaagaaaa aataaacaga tcgatttcca gcgatttaag
     7621 ttgggaaatc agcgagggat tgacgtaaca gccatatttg cgctcaagtg tgccgccgga
     7681 actgatcagt tgaatgcgtt cgcgtttacc gagttcatgg caaatcggat ggctattggt
     7741 gaagacctga atgttgatgt caggcaactg gcgtgccaga taccagcaag ttgaactggc
     7801 gtctaaggct atcaccatcc cttcttcaat ccacgcgagc gcctcgcgcg cgatatctgc
     7861 tttatgcgca taatggcttt tcagcctgat gtgaaaggga tcgccactgt cttgattttg
     7921 acggtggata tatttagcgc gtccatgatt gcgcagaatt ttaccctgcg tctgtaattc
     7981 attgagatcg cgacgaatgg tttctttact gacctttagc tgttcagaga gagcttccgt
     8041 ggtcaggctg gtatggttca gcagcaggtc gactatcgct tgctggcgtg ccgctttcat
     8101 atttttcacc ccatcattcg ataaccgccc gcgtcatgcg ggcgtaccgg tatgagatta
     8161 cggtgttacc ccttttttta aaagaatatt cccgtacttc gctcgttcgc ctgtgataac
     8221 gatcgcaaag gctttttgcg cccgttcata aaacgcaaaa cgattgatgc ggatgatgtc
     8281 aggacacggg gcttgtagtg aaagcgcatt acggtaacgt cgttctactt caggatcgag
     8341 agtgtcaccc tctaccgccg ccatcatcac cagcggcggg gcataactgt ccagctcaaa
     8401 taacgggata atcgcctgga gcaagtcgct caccaacagg ccatcagcgc ggatcacctg
     8461 cggttccatc gaatgggcgg gaaagtgagc atcggaaaaa ataatttcat ctccatgtcc
     8521 catctctgcc agcactttca atagttcggg agaaattaac ggcgaaattg ttttcagcat
     8581 ttcacacttc ctctataaat tcaggttcag tttgcgggta gaaataacgg tactgataat
     8641 gaatctgtgc acgggcttct tccgggctgt taaattcccc tacgccatac caaccgaaca
     8701 gcgcagctcc tgcgacggtc gtttcggcat cgtcgagaac ttttaccgga atatcaagca
     8761 tattggcttt aatctgattc cacaatgtgt tgcgacttcc tccaccgact aacaataatt
     8821 cataggcttt aaagtgcccg attttttcca gcatctgtag attgcgctgt aattgcgcag
     8881 ttaacccttc cagcgccgcg cgatagaaat gcccccgcgt ggtattaagc gtcactcctt
     8941 gccagccagc gttctgacac gacaataaat cacactgcat tttcacacca tccgcgccag
     9001 gtgcgatcag acgagcttct tcaatcaaca tttgccaggg tgtttcagcc gtccagaaca
     9061 gttttctcac ccattccagc acgccggatg ccagccattg catacctggg ttatacaacc
     9121 atgcctggct atctagttcg caggtggaac cggcgtactg acttaacagc gaagtatcaa
     9181 cctaggcgct gcgaaccatt aaaatttccc atgtaccgga ggagagcacg ggttcatttt
     9241 gttcggcacc agcgccaaaa agggcgaact gggtatcgtg acctgcggaa atcaccggta
     9301 tgccaacggg taagccgagc attgctgcgg cgttgttctg tagcgtacca atctgttcac
     9361 ccgcttccac cagacgaggg aagagtcggc gtggaatacc ggtggcttgt aaaatttgcg
     9421 gactgaaatc gcgttgctgg atatccagca tctggctggt tccggccatc gtgatatcag
     9481 tagtgaattc gctggttaaa cggtggttaa tcagcgacga aatgaagagc caggcgtgcg
     9541 cgcgttccag cagttgtgga tgattttctt tcaaccacac caacttatat aacgtattga
     9601 aactaaaggc tccgacgcca gaaatagcct gcaaccgctg tgcggagatt aaccgttcaa
     9661 tattgtccat aaccgctgct gttcgcggac atttccagct aatgatcgga tagagcagat
     9721 tgccttgctt atctaccaga gcgccatcca cactaaaggt ggtgacggcg ataccgcgga
     9781 tgtggcattc agtcagttca ctattgattt gccgacagca atcagcaaag cgttgcaaaa
     9841 tggcagtcta aagaccactg gtgccaggtg ttgttttcca tcgcgatatc gctggcatta
     9901 ggcgttgagg cgcgggcaac aatttttccc tgccgattaa ccgcgatggc cctgacattg
     9961 gtcgcgccac agtcgagtac caggataact tcttgtttca taatcgctcc tgcaatatag
    10021 ccggataaca ttgtttatcc ggctaaccac tcttgcttgt tcaggcccgg tagccggagc
    10081 gaccgggcat cacatcaggg tgtaatgtat taacgcttgt acaacggacc gtagttctgg
    10141 caagcgcggt aatcctggcc ttcaatatcc atgccgtgcg cagcccaggc agaaggacga
    10201 tacactttgg tctcttcaac gttgtgcata cataccggga tacgcagcat ggaggcaagg
    10261 gtgataaagt ctgcgccaac gtggccgatg gtcagaactc cgtggttggc accccagttc
    10321 gccattaccg aatacacatc cgtaaacggc cctttaccag tgaggcgcgg tgcaaaccag
    10381 gtggttggcc aggttgagtt ggtgcgtttg ttgaggatgt catgcacatc cttcggcaat
    10441 tccacgctcc agccttccgc gatttgcagt accggtccca ggcctttgat gatgttgaca
    10501 cgagtcatgg tgaacgggac gccgccttcg gtaaggaagc gggaagagta accgccgcca
    10561 cggaagtatt cgtggatcgc cgggcaccat tcggtagcgg cgaggcaagc gtcagcctct
    10621 tgctgagaga tttcccagtg cggcttcatc gtcgggttac cttcgctgtc gcgttgttta
    10681 caggaaccgt ccagcgcagc agaacctgag ttgatcaaat ggatgatgcc gtgttctgcc
    10741 agtccgtcca gtttatgccc cgttacacgc tcaattgctt ctggtgacca gtaggtacgc
    10801 acatcggcaa atacctgagc ggtgccggtg agctggtgac ccattagcat tgccacgccg
    10861 ttaagactgt cgttttcggt cgccacgaca aagggttcgc gcacgccatt ccagtcaaat
    10921 gaactgttga ggatcgcttc ggcggtgtca ccattgggat attgatcggt ccagtgacgt
    10981 tgcccctgga agcccgcagc gatggcgttg tagccaagtg attcttccac gcgaccaata
    11041 tcggccagtt tgctgttgcc ttgcatcatg tcgcggatac acatcgccat cagtaaactt
    11101 tcgcgcagaa ctgcgcggct ttgctcggca ttgcgttgat actgtttgtt attttcatct
    11161 tcgccatagc ggaagttttt atcagcccag gccagtgcca tttccagttc ggcttcgtcg
    11221 taaatcttct gatcgatacg gcgacgcagt tcggtcatat ccaccgcctg gactttcatt
    11281 cccagccagg attcaaagaa gttgtgatca acaatagaac cggcgatacc catcgaaacg
    11341 ccgcccagcg acagatagct tttacctttc atgctggcga cggccaaacc ggcgcgggca
    11401 aagcgcagca gtttttcttc aacatcggca ggaatcgatg tgtcatcggc atcctgaacg
    11461 tcatgaccgt aaatggagaa tgccgggatg cctttctggc tgtgagctgc cagagccgca
    11521 gccaggtaaa cagcgccggg gcgttcagtg ccgttaaagc cccaaatggc tttcgggcgg
    11581 gttggatcca tgtcgatggt ttcactgcca tagcaccagc aaggcgttac cgtaatggtg
    11641 aggcctacat tctggctgct gaatttttct tcgcaagcag cggcttcagc catacccgcg
    11701 atacaggtat cggaaatgac acactcgacg gcagctccgc aggcatggcg cagtttctcg
    11761 gtcagcagtg cggccgtagc tttcgccata ttcattgttt gttcttcaag cgactcacga
    11821 acacccatgc gacgaccgtc aataaccggg cggataccaa ttttcggtaa gctgattttt
    11881 ttcattacag attcctcact ttattcggaa aatatgttca gttagttgcc gtttgagaac
    11941 ggaaacgggc aaagataaag atgaccgcga agcagagtgc ggggatcagt tcagcagtgg
    12001 ggatgttgcc cgccgcgtca ctgacaaaac ccatgaccgg agtgacaata ccgccgccaa
    12061 taatggtcat aacgatgaag gacgaaccgt atttggtgtc ctggccgaga ttcttaatgc
    12121 ccagcgagaa gattgttggg tactgaatcg acataaaggc gctgcataaa gtcagggcta
    12181 ttaagcccac atgaccgcca gcgaaggctg agatcaggca cagtgccata gcgattaatg
    12241 cgtaggcggc caggactttg tgtggtgcga agcgactgat gagccaggta ccggtgaaac
    12301 gaccaataaa gaagcacacc atggttccgg ttaaatagtt agcggcaaag cctgcagtca
    12361 tacctagaat ttcttctaca gcgtagcgaa tcaaatagct ccagcaggcc gtttgtgcgc
    12421 cgacatagca gaattgcgct aataccgccc agcgccagtg acgaatacgc gccagacgag
    12481 aaagcgatgc ggagaacgat ccttgtttgg cgtcactgtg attatcactc tgcaatgccg
    12541 ggaatttcgt cagcatgatc agcagggcga ccagtaacac gatagccacg atgatcatat
    12601 aaggtgtctg taccgataat accaggctgt gtttatacgc actcaattgc tctggagaca
    12661 ttttatcgag aacgtcttgc gattgatgtg gcacgttaga caaaataaga ctttgcccaa
    12721 agacaaccgc gataattgcg ccaaacgagt taaatgtttg cgcaagattt aagcggaagt
    12781 gactactgct ttccggccct aataccgtaa caaaagggtt tgcggcagtt tccagacaac
    12841 ctaatccggc tgcaataata aataggccaa ctaaaaataa ggtgtagttc attatttctg
    12901 cagcgggcca gaataatgca gcgcccaagg catataaaaa taatccggta ataatccctg
    12961 ctttataact gagttttttc atcaatatcc cagcagggat tgggataatg aaataaccaa
    13021 agtaaaaggc cgattggatc aggccagcct ggaaatttgt cagcgtaaaa gcctgctgga
    13081 attgaggtaa taaaatgtcg ttaaggttat tggctaccgc ccaaagaaaa aacagtgagc
    13141 acagcagcgc gaatggaata atgtaacttc tgctttgccc tgcatcttta tctaccgcac
    13201 ggtaactctg cgtttgtatt gatgtgtttc ccatagcatc ctcttaggtt cagaagctta
    13261 agcacgtaat cggttatgtt ttcaaaagtg caaaaccagc gagttcgggc ttcatatcgg
    13321 tgaatatttg cctgtctgaa atgaactcat ggattcattt tcaaataaaa aacgctctgt
    13381 aatatgacgg cggtcacact tattagctgg aaggtgactt tatgtgacta ccatcacttt
    13441 aataatcgaa atttcatgcc cgtttcagaa aataaatcat aaaaaacaca aaccagatga
    13501 ccgtattaat tgtttgtgcc gaaaacgggc atttctggcg aggggaaatg gaaaaatgac
    13561 cgttttgata aatattttta agagcggtca tttttaacgg ggttataaaa agagaacttg
    13621 cagttattta ctgtgattag ttgagctagg tcacaaaata taagcttgtg aatttctttc
    13681 ttgttaatca ggaagaagat ggtgggtaat taaacggcta attcaatagt gtgaaaggaa
    13741 caacattatt gccctgtttt gaatcagaga gaggtagcta atggaacgaa ataaacttgc
    13801 tcgtcagatt attgacactt gcctggaaat gacccgcctg ggactgaacc aggggacagc
    13861 ggggaacgtc agtgtacgtt atcaggatgg gatgctgatt acgcctacag gcattccata
    13921 tgaaaaactg acggagtcgc atattgtctt tattgatggc aacggtaaac atgaggaagg
    13981 aaagctcccc tcaagcgaat ggcgtttcca tatggcagcc tatcaaagca gaccggatgc
    14041 caacgcggtt gttcacaatc atgccgttca ttgcacggca gtttccattc ttaaccgatc
    14101 gatccccgct attcactaca tgattgcggc ggctggcggt aattctattc cttgcgcgcc
    14161 ttatgcgacc tttggaacac gcgaactttc tgaacatgtt gcgctggctc tcaaaaatcg
    14221 taaggcaact ttgttacaac atcatgggct tatcgcttgt gaggtgaatc tggaaaaagc
    14281 gttatggctg gcgcatgaag ttgaagtgct ggcgcaactt tacctgacga ccctggcgat
    14341 tacggacccg gtgccagtgc tgagcgatga agagattgcc gtagtgctgg agaaattcaa
    14401 aacctatggg ttacggattg aagagtaatt tcgtaaagca acaaggagaa ggatgatggc
    14461 taacagaatg attctgaacg aaacggcatg gtttggtcgg ggtgctgttg gggctttaac
    14521 cgatgaggtg aaacgccgtg gttatcagaa ggcgctgatc gtcaccgata aaacgctggt
    14581 gcaatgcggc gtggtggcga aagtgaccga taagatggat gctgcagggc tggcatgggc
    14641 gatttacgac ggcgtagtgc ccaacccaac aattactgtc gtcaaagaag ggctcggtgt
    14701 attccagaat agcggcgcgg attacctgat cgctattggt ggtggttctc cacaggatac
    14761 ttgtaaagca attggcatta tcagcaacaa cccggagttt gccgatgtgc gtagcctgga
    14821 agggctttcc ccgaccaata aacccagtgt accgattctg gcaatcccca ccacagcagg
    14881 tactgcggca gaagtgacca ttaactacgt gatcactgac gaagaaaaac ggcgcaagtt
    14941 tgtttgcgtt gatccgcatg atatcccgca ggtggcgttt attgatgctg acatgatgga
    15001 tggtatgcct ccggcgctga aagccgcgac gggtgttgat gcgctcactc atgctattga
    15061 ggggtatatt acccgtggcg cgtgggcact aaccgatgca ctgcacatta aagcgattga
    15121 aatcattgct ggggcgctgc gaggatcggt tgctggtgat aaggatgccg gagaagaaat
    15181 ggcgctcggg cagtatgttg cgggtatggg cttctcgaat gttgggttag ggttggtgca
    15241 tggtatggcg catccactgg gcgcgtttta taacactcca cacggtgttg cgaacgccat
    15301 cctgttaccg catgtcatgc gctataacgc tgactttacc ggtgagaagt accgcgatat
    15361 cgcgcgcgtt atgggcgtga aagtggaagg tatgagcctg gaaggggcgc gtaatgccgc
    15421 tgttgaagcg gtgtttgccc tcaaccgtga tgtcggtatt ccgccacatt tgcgtgatgt
    15481 tggtgtacgc aaggaagaca tttcggcact ggctcaggcg gcactggatg atgtttgtac
    15541 cggtggcaac ccgcgtgaag caacgcttga ggatattgta gagctttacc ataccgcctg
    15601 gtaaatgcgc tgatgtgata atgccggata cgatttgcgg cgtatccggc tcgccgttac
    15661 cgtaccaacc gcaattgctg taaattgccg tcgatatgca aatcggtttg taagcgggca
    15721 atatcgcggc acagaaacgc catctcttta tgggtttcta attttttgcg ccacttttcg
    15781 gcaaccgcat ccagattctc atatatccct tccagactct gaaactcgac cagcagctgc
    15841 gtggcgcttt ttggtccgat tcccgcaaca cccggtacct ttgaactgct gatccccgca
    15901 agtccccagt aatcgggcaa ctgctgcggt tgaacgccaa attctttatc gataaatggc
    15961 gcatccagcc aacgtttctg gaagtaatca cgaatacgta atgtcggtga aagtaactga
    16021 cagtagcctt tatctgtcga aacaatcgtt gcctgatgcc cggcctgtgt cactttgacc
    16081 gccagtgtgg cggctaagtc atcggcttcg ttgccgctgg ctgaccagca cgggacgccg
    16141 cgttgctcaa aggcggcgcg taatgcaggc atctcgtcgt gcaactcttc cggcattggc
    16201 ggtcgatccg ctttgtaatc tggtaaacgc tgatgacgcc agccgctact gcggttttca
    16261 tcatcaaaaa cggcgaccgc gtgggttggc tggctgtgca taatgagctg atcgagcgca
    16321 tgctggcagg tttcgacaca gggcgaaccc tgaacggcat gaatgcgacg gataagattc
    16381 agtgcatcga caataagcaa atgaacagcc acgataaccc ccttaaagat caggcgataa
    16441 gggtaacatt cgatgccgga acaggctatg gctggttgag aaaacaggaa gaggcttcgc
    16501 aaaacgaggc ctctgggaga gattaatcgc aggcaacgat cttcatcgcc aggccgccgc
    16561 gagaggtttc gcggtacttg gcgttcatgt ctttacctgt ttcgtacatg gtttcgataa
    16621 ctttatcgag gcagacgcgc ggttcgctgg tacggcgcag tgccatacgc gcggcgttca
    16681 ccgctttcac tgctgcaatg gcgttacgct caatgcacgg tacctgaacc tgccccgcga
    16741 ccgggtcaca agtcagaccg aggttgtgct ccatcgcgat ttccgctgcg atgcaaacct
    16801 gcgccgggct tgcgcctaac agttctgcca gaccagctgc cgccatagag cacgccacgc
    16861 caacttcacc ctggcaaccc acttcggcac cagaaatcga cgcgttcatc ttataaagag
    16921 aaccaatggc gctggctacc agcaggtaac gagccagtga gttagcgttc acttcgcgga
    16981 taaacttatc gtagtacgcc agcacagccg ggataatccc gcacgcaccg ttagtcggcg
    17041 cagtcaccac gcgaccgcca gcagcgttct cttcgttcac tgccagtgca aacatgttga
    17101 tccagtcaac aaccgccatc gggtcagtgg tggttttatc ctggctgacc agcatccggc
    17161 gtagtgccgc agcacggcgt ggaacgcgca gtttgccagg caacacgcct tcggtggaaa
    17221 taccgcgctc aataccgcca cgcatgactt cccagacgtt cgccaggtgc tgttccagct
    17281 cttctttact gtgcagcgcc agctcgtttt tcatcatcag gccagagagt gacagcccgg
    17341 tttcctgaca atgtttttgc agatcggctg ctgaactgta cggataagga acttcaaccg
    17401 gtgcgctatc ctgctggcca aaatgctctt catcaacgat aaagccaccg ccaatagagt
    17461 agtaagtctg gctgtaaacg actttatcgc ccgccagcgc ggtaatgcgc ataccgtttt
    17521 catgcagaga aaggttgtct gcatggaagt tcatgcactg atcaaccggg aactccactt
    17581 catgctgacc gtttgccagc atcaggcgac catgagtatt cacatcctga ataaaaccgg
    17641 ggatggaatc gatatccacg gtatccggca ggttacccgc caggcccata ataatggcga
    17701 tatctgtgtg gtggccttta ccagtcagag agagcgagcc gtacacgtca accaccacgc
    17761 gggtcacgtc tttaagcagg ttacgggcaa tcagatcgtc ggtaaattgt ttacccgctt
    17821 tcattggtcc aacggtatga gaactggaag ggccaatgcc gattttgaaa atatcgaata
    17881 cgctaatcat aggaaataca tcgcgttaaa acggaggaag cgccgcccga aagcggcgcg
    17941 aaaggactta gctgaacaga gagtagaaga ttgcggagat tgcaatcaga cccatcacga
    18001 caacgaatac gttgctgatg tgaccgctgt acttacgcat tgccggtact ttctgaattg
    18061 cgtacatcgg catcaggaac aggatcatcg cgatgattgg gccgcccagg gtttcaatca
    18121 tacccaggat gctcgggttc agggtggcaa caatccaggt cgttaccagc atgaacagcg
    18181 cagtgatacg gttcagcttg ttgatttcga tagacttacc tttaccacgc agagatttaa
    18241 tcaccatacc gttgaagcct tcacgtgcgc ccaggtagtg gccgaggaag gatttggtga
    18301 tagcgataat cgcgataatc ggagccatcc acgcgataac cggtgcgtta aagtggttag
    18361 ccaggtaaga cagaatcgag atgttctgct ctttagccgc agccaggtct gccggagtca
    18421 ggctcagtac gcagctgaag acgaagaaca ttacggtcag caccatcatg atgtgtgcga
    18481 atgccaggat cttcgagcat ttctgttctg ccatatcgcc gtactcttca cgcttcgcaa
    18541 cggcgaaaga agagatgatc ggagagtggt tgaacgagaa caccattacc ggaattgcca
    18601 gccacagggt catccacaga ccgtttccgg ttgcagatgc agtgtccaga gacagcgttt
    18661 ccagtgctgc gccgttccac tgcgggatca ggtacagagc cagcagcatc agtacgccaa
    18721 caaacgggaa taccagaata ctcatcgctt taacgatcat ctgctcacca aagcgaacga
    18781 tggtcatcat acccacgatc aggatcagcg acagaatcgc acgcggcggt ggcgtcatac
    18841 ccagctggtg agacatgaag ctttcaacgg tattggtgat tgccacgcta taaaccagca
    18901 ggatcgggta gatagcgaag aagtagagca gggtaatcag tttacctgcg ccaataccaa
    18961 agtgttcttc tacaacctcg gtgatgtctt cgcccgggtt tttaccagac agtacgaagc
    19021 gagtcaggcc gcggtgagca aaaaacgtca tcgggaacgc aaggatagcc atgatgatca
    19081 gcgggatcat accgccaaca ccggcgttga ttggcaggaa cagcacgccc gcgccgattg
    19141 ccgtgccgta aaggcccagc atccacatgg tgtctgtctt gcgccaggca ctacgagagt
    19201 ctttcgacgc aatcgtgctg gtttgagtcg tttccatcta tttctcctgg aggaatgcaa
    19261 ataattcagc ttttagccag ttcagcccgg gaaggtgcct gaccgtaaaa atgaaatctg
    19321 tctatcggcg atggcttaat catttaccgc cgtaataatt tgagggcgga aagatacaca
    19381 caagttatgt atctatcagt gatcttgatc ccaaatacaa taatctgctt ggaagataga
    19441 ttatcccaac gctttactct gtaaatcaaa agtaaaagag aatttaaggg caatatttgc
    19501 ccattaaacg acatctgact tcttccgggg cgcgcaatat atgtaggttg aactgaaaaa
    19561 atcaatactt ttagttaatc atggcgataa tttctttttc atagcgataa aatacggttt
    19621 atgaaaatag ggaaacgttt gtgttgcaac ataaattgta tgacatatta actgtgatga
    19681 tatttgtcgc gtgagggtgg taagaaacat tcatataaca aatgaaatga gttgatgagt
    19741 aagtgaccaa aaacgagaaa acccggcaa
//
LOCUS       DAJTSF010000077        19698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000077 DAJTSF010000000
VERSION     DAJTSF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.077"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO335_002837"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO335_002837"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6003157.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO335_002838"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO335_002838"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6003158.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO335_002839"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO335_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6003159.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="OO335_002840"
     CDS             6038..6658
                     /locus_tag="OO335_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6003160.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="OO335_002841"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="OO335_002841"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6003161.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO335_002842"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO335_002842"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6003162.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO335_002843"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO335_002843"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6003163.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="OO335_002844"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="OO335_002844"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6003164.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO335_002845"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO335_002845"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="OO335_002846"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="OO335_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="OO335_002847"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="OO335_002847"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6003165.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="OO335_002848"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="OO335_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6003166.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO335_002849"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO335_002849"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6003167.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="OO335_002850"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="OO335_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6003168.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="OO335_002851"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="OO335_002851"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6003169.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="OO335_002852"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="OO335_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6003170.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4683 a         5506 c         5031 g         4478 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DAJTSF010000078        19486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000078 DAJTSF010000000
VERSION     DAJTSF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.078"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..158
                     /locus_tag="OO335_002853"
     CDS             <1..158
                     /locus_tag="OO335_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6003171.1"
                     /translation="ALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGT
                     FYTPQGS"
     gene            184..2274
                     /gene="pbpC"
                     /locus_tag="OO335_002854"
                     /pseudo
     CDS             184..2274
                     /gene="pbpC"
                     /locus_tag="OO335_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; incomplete; partial
                     in the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2424..2855
                     /gene="ndk"
                     /locus_tag="OO335_002855"
     CDS             2424..2855
                     /gene="ndk"
                     /locus_tag="OO335_002855"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6003172.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            3005..4159
                     /gene="trmG/rlmN"
                     /locus_tag="OO335_002856"
     CDS             3005..4159
                     /gene="trmG/rlmN"
                     /locus_tag="OO335_002856"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6003173.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4444..5457
                     /gene="rodZ"
                     /locus_tag="OO335_002857"
     CDS             4444..5457
                     /gene="rodZ"
                     /locus_tag="OO335_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6003174.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5484..6611
                     /gene="ispG"
                     /locus_tag="OO335_002858"
                     /gene_synonym="gcpE"
     CDS             5484..6611
                     /gene="ispG"
                     /locus_tag="OO335_002858"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6003175.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6714..7988
                     /gene="hisS"
                     /locus_tag="OO335_002859"
     CDS             6714..7988
                     /gene="hisS"
                     /locus_tag="OO335_002859"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6003176.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            8006..8626
                     /gene="yfgM"
                     /locus_tag="OO335_002860"
     CDS             8006..8626
                     /gene="yfgM"
                     /locus_tag="OO335_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6003177.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8637..9815
                     /gene="bamB"
                     /locus_tag="OO335_002861"
     CDS             8637..9815
                     /gene="bamB"
                     /locus_tag="OO335_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6003178.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9933..11405
                     /gene="der"
                     /locus_tag="OO335_002862"
     CDS             9933..11405
                     /gene="der"
                     /locus_tag="OO335_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6003179.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11475..11690
                     /locus_tag="OO335_002863"
     CDS             11475..11690
                     /locus_tag="OO335_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6003180.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11687..13057)
                     /gene="xseA"
                     /locus_tag="OO335_002864"
     CDS             complement(11687..13057)
                     /gene="xseA"
                     /locus_tag="OO335_002864"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6003181.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13219..14685
                     /gene="guaB"
                     /locus_tag="OO335_002865"
     CDS             13219..14685
                     /gene="guaB"
                     /locus_tag="OO335_002865"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6003182.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14754..16331
                     /gene="guaA"
                     /locus_tag="OO335_002866"
     CDS             14754..16331
                     /gene="guaA"
                     /locus_tag="OO335_002866"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6003183.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16426..16965)
                     /locus_tag="OO335_002867"
     CDS             complement(16426..16965)
                     /locus_tag="OO335_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6003184.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16981..17499)
                     /locus_tag="OO335_002868"
     CDS             complement(16981..17499)
                     /locus_tag="OO335_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6003185.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17810..18001)
                     /locus_tag="OO335_002869"
     CDS             complement(17810..18001)
                     /locus_tag="OO335_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR6003186.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            18019..18171
                     /locus_tag="OO335_002870"
     CDS             18019..18171
                     /locus_tag="OO335_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003187.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18353..>19486
                     /gene="pdeF"
                     /locus_tag="OO335_002871"
     CDS             18353..>19486
                     /gene="pdeF"
                     /locus_tag="OO335_002871"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6003188.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4589 a         4754 c         5296 g         4847 t
ORIGIN      
        1 ccgcgctcag ggatccgctc tccagggctt actacacccg caaaatgagt cagggaaaac
       61 gacacaatca ggcgcttatc gccctggcga gacgacgctg cgacgttctg ttcgccatga
      121 tgcgcgacgg gactttttat accccgcagg ggtcataaca tgcttgacaa cttaatagag
      181 gcacgttacc ttgaagcgct gatcaattat gaagatcgct ggttctggaa gcatccgggg
      241 gttaatccat tctcggtggc gcgcgcagca tggcaagatc tcacttcggg acgggttatt
      301 tccggtggca gcacgctcac tatgcaggtt gctcgtctgc ttgatcctca ccccaaaaca
      361 tttggtggca aaattctcca gctctggcgc gcgttgcaac tggagtggca tttgtctaag
      421 cgtgaaattc tgatcttgta tcttaaccgc gctccgtttg gcggtacgtt gcaggggatc
      481 ggtgcggcaa gttgggctta tctcggaaaa tcgcctgcga atttaagcta ttccgaggcg
      541 gcaatgctgg cggtgttacc gcaagtgccc agccgactgc gcccggatcg ttggccggag
      601 cgtgccgaag ccgcgcgtaa taaagtgctc gaacggatgg ctgcgcaagg tgtgtggtcc
      661 cgtgagcagg taaaagagtc aagggaagaa cccatctggc tggcaccccg acaaatgccg
      721 caactggcac cgctgttttc gcgcatgatg ctcggtaaaa gcaaaagcga caaaatcgtt
      781 actacgctgg atgccggtct tcaacgacgt ctggaagaac tggcgcaaaa ctggaaaggg
      841 cggttgccac cgcgcagctc actggcgatg atcgtggtta atcataccga tatgcgtgtt
      901 cgcggctggg tgggatcggt tgatctcaac gatgattcac gctttggtca tgttgatatg
      961 gtcaatgcga tccgatcgcc aggatcggtg ctcaaaccgt ttgtttatgg tctggcgctg
     1021 gatgaaggct tgatccaccc ggcatcactg ctgcaagacg tcccccggcg caccggtgat
     1081 tatcgaccag gtaactttga tagcggtttt catggcccga tcaacatgag cgaggcgctg
     1141 gtgcgctcgc tgaacttacc tgctgtgcag gtgctggaag cctatggacc gaaacggttt
     1201 gcggcaaagt tacgcaatgt tggattgccg ttatatttgc ccaacggcgc tgcgccgaat
     1261 ctttcactca ttctcggcgg cgctggtgca aaactggaag atatggcggc agcgtatacc
     1321 gcgtttgctc gccacggcaa ggcaggcaaa ttgcgcttac agcctgacga tccgctgctt
     1381 gaacggcctt taatgtcgtc gggggcggcg tggatcattc gccggattat ggctgatgaa
     1441 gcgcaaccct tgccggatag tgccttgccg cgcgtcgccc cactggcatg gaaaacgggc
     1501 accagctatg gctatcgtga cgcctgggcg attggggtta acgctcgcta tgtcattggg
     1561 atctggactg gcagacctga cggcacgccc gttgttggtc agtttggctt tgccagtgcc
     1621 gtaccattgt tgaatcaggt caataatatc ttactgtcgc gcagtgcgaa tctgccggaa
     1681 gacccgcgcc cgaactcagt cactcgtggc gttatctgct ggccgggcgg acagtctttg
     1741 ccggaaggtg acggtaactg tcgccgccgc ctggcaacct ggctgctgga tggcagccag
     1801 ccgccaacgc tgttactgtc agagcaggaa gggattaatg gcattcgctt ccccatctgg
     1861 ctggatgaca aagggcagcg cgtcgccgct gactgcccgc aggcgaagca agaaatggta
     1921 aatgtctggc cgctaccgct ggaaccctgg ctgcccgcat cagaacgtcg cgctgtgcgc
     1981 ttaccaccgg cctcgacaat ttgcccgcca tacggtcacg atgcccagct ccctctgcag
     2041 ctgacaggcg ttcgcgatgg cgcgattatt aaacgtttac cgggcgcagc ggaagcgact
     2101 ttgccgttgc aatcaagtgg aggggcaggt gaacgctggt ggtttctgaa tggcgaaccg
     2161 ttaactgaac gcgggcgcaa cgtgaccctg catttgacgg ataaaggtga ttatcaattg
     2221 ctggtgatgg atgatgtggg gcaaatcgcg acagtgaaat ttgtcatgca atagtctgtt
     2281 ttgtccctga ttgttgctaa aactcatttt attttaaaaa aaatgttacc ttcatcaata
     2341 gtcaacggcc ctgttgctca ttataatccg cgccatctcg tacgctggta cagacaacaa
     2401 cagaacaatt tacagaggta aaaatggcta ttgaacgtac tttttccatc atcaaaccga
     2461 acgcggtagc aaaaaacgtc attggtaata tctttgcgcg ctttgaagct gcagggttca
     2521 aaattgtcgg caccaaaatg ctgcatctga ccgttgaaca ggcacgtggc ttttatgctg
     2581 aacacgatgg aaaaccgttc tttgatggtc tggttgaatt catgacctct ggcccgatcg
     2641 tggtttccgt gctggaaggt gaaaacgccg ttcagcgtca ccgcgatctg ctgggcgcga
     2701 ccaatccggc aaacgcactg gctggtactc tgcgcgctga ttacgctgac agcctgaccg
     2761 aaaacggtac ccacggttct gattccgtcg aatctgccgc tcgcgaaatc gcttatttct
     2821 tcggcgaagg cgaagtgtgc ccgcgcaccc gttaataatt tcgtaaatgc cgcgtgcaaa
     2881 cgtggcatcc gtgcgccaga atttgtacaa tgcagcgccc ccggacgagc agccgctcac
     2941 cggggcgttt cttttttcaa ccctccaggg gccataacgt gtaataacga ggccggaata
     3001 gcatatgtct gaacaattag tcacacctga aaacgtcacc acgaaagatg gaaaaatcaa
     3061 cctgctggat ctcaaccgtc agcagatgcg ggagtttttt aaagatttag gtgaaaaaac
     3121 cttccgcgcc gatcaggtga tgaagtggat gtatcactat tgctgcgaca actttgatga
     3181 gatgaccgac atcaacaaag tgttgcgcgg caaactgaaa gaggtggcgg aaatccgcgc
     3241 accggaagtg gttgaagaac agcgttcatc cgacggcacc attaaatggg cgatcgccgt
     3301 tggcgatcag cgcgtcgaaa cggtgtatat cccggaagac gaccgtgcca cgctctgcgt
     3361 ctcttcgcag gtggggtgtg cgctggagtg taaattctgt tccaccgccc agcagggctt
     3421 taaccgcaac ctgcgggtat cggaaattat tggccaggtg tggcgtgcgg cgaaaatcgt
     3481 cggcgcagca aaagtcaccg gtcagcgccc aatcactaac gtcgtgatga tgggcatggg
     3541 cgagccgctg ctcaacctga acaacgtcgt tccggcgatg gaaatcatgc ttgatgattt
     3601 cggttttggc ctgtctaaac gtcgcgtgac gctttccact tccggtgtag ttccggcgct
     3661 ggataaactg ggcgatatga tcgacgttgc actggcaatt tccctgcacg cgccgaacga
     3721 cgaaattcgt gacgaaattg tgccgatcaa caaaaagtac aatatcgaaa cgttccttgc
     3781 tgcggtgcgc cgttatctgg agaaatccaa cgccaatcag ggccgagtca ctattgaata
     3841 cgtgatgctt gaccacgtta acgacggcac tgaacacgcg caccaactgg cggaactgct
     3901 gaaagatacg ccgtgtaaga tcaacctgat cccgtggaac ccgttcccgg acgcgccgta
     3961 tggacgcagt tcgaacagcc gtatcgaccg tttctcaaaa gtgctgatga gctacggttt
     4021 caccactatt gtgcgtaaaa cgcgtggtga tgatatcgat gctgcctgtg gtcagttggc
     4081 gggcgatgtt atcgaccgta cgaaacgtac tctgcgtaaa cggatgcagg gtgaagccat
     4141 cgatattaaa gcggtctgat agccattacg ccacggttac tttatgtacc gtggcgtaat
     4201 cttttatgaa tgaagtatta agttgcaatt gtctggtcaa tattaacggt gcgtttttgc
     4261 tgactttaag gcagtatgta acgatgcaac agtaagttag cctcagtgaa tgtgggcttt
     4321 gtcacgagca cacagacggt cttatactgt atgataacgg ttaacttaac ggatgtttcg
     4381 cggtgtgggt gagcatgatg gttcaccggc atctcaattc tcatttaaac gtacctgcag
     4441 cgaatgaata ctgaagccac gcacgaccaa aatgaagcac ttactaccgg cgctcgcctg
     4501 cgtaatgctc gcgaacaact aggacttagt cagcaggccg ttgccgagcg actttgcctg
     4561 aaggtttcca cggtacgcga cattgaagaa gataaggcac ccgccgatct tgcttcaaca
     4621 ttcctgcgcg gatatatccg ctcttatgcg cgtctggtac atattccaga agaagaactg
     4681 ctgccagggc tggaaaagca ggctccactt cgggctgcaa aagttgcgcc aatgcagagt
     4741 ttttccctcg gtaaacgccg caaaaaacgc gacggctggc taatgacctt cacctggctg
     4801 gtgttgtttg tggttatcgg cctgagcggt gcctggtggt ggcaagacca caaagctcag
     4861 caggaagaga tcaccactat ggccgatcaa tcttcggcgg aactgagcag taatagcgag
     4921 caggggcaga gtgttccgtt aaatacgtcg acaactacag acccggctac aacgtccacg
     4981 ccgccagcgt ctgtggatac taccgcaacc aacacgcaaa cacctgccgt aactgcgcca
     5041 gcaccagctg ttgatccgca acagaatgcg gttgtttcgc cttcgcaggc aaatgttgat
     5101 accgccgcga ccccggtacc aacggcagca acaacgccag atggtgctgc gccgttgcca
     5161 accgatcagg ctggcgtgac cacgccggcg gctgatccga atgcgctggt gatgaacttt
     5221 actgccgatt gctggctgga ggtcactgat gctaccggta aaaaattgtt tagcgggatg
     5281 cagcgtaaag acggtaattt gaatttaacc ggccaggcac cgtataaact gaaaattggt
     5341 gcgccagccg cagtacagat ccagtatcaa gggaaacctg tcgatctgag tcgttttatc
     5401 agaactaacc aggttgcgcg tctgaccctc aatgccgaac aatcaccggc gcagtaacag
     5461 acgggtaacg cgggagattt ttcatgcata accaggctcc aattcaacgt agaaaatcaa
     5521 cacgtattta cgttgggaat gtgccgattg gcgatggtgc tcccatcgcc gtacagtcca
     5581 tgaccaatac gcgtacgaca gatgtcgaag caacggtcaa tcaaatcaag gcgctggaac
     5641 gcgttggcgc tgatatcgtc cgtgtctccg taccgacgat ggacgcggca gaagcgttca
     5701 aactcatcaa acagcgggtt aacgtgccgc tggtggctga catccacttc gactatcgca
     5761 ttgcgctgaa agtagcggaa tacggcgtcg attgtctgcg tattaaccct ggtaatatcg
     5821 gtaatgaaga gcgtattcgc atggtggttg actgtgcgcg cgataaaaac attccgatcc
     5881 gtattggcgt taacgccgga tcgctggaaa aagatctgca agaaaagtat ggcgaaccga
     5941 cgccgcaggc gttgctggaa tccgccatgc gtcatgttga tcatctcgat cgcctgaact
     6001 tcgatcagtt caaagtcagc gtgaaagcgt ctgacgtctt cctcgctgtt gagtcttatc
     6061 gtttgctggc aaaacagatc gatcagccgc tgcatctggg gatcaccgaa gccggtggtg
     6121 cacgcagcgg ggcagtaaaa tccgccattg gtttaggtct gctgctgtcg gaaggcatcg
     6181 gcgacacgct gcgtgtatcg ctggcggccg atccggtcga agagatcaaa gtcggtttcg
     6241 atattttgaa atcgctgcgt atccgttcgc gagggattaa cttcatcgcc tgcccgacct
     6301 gttcgcgtca ggaatttgat gttatcggta cggttaacgc gctggagcaa cgcctggaag
     6361 atatcatcac tccgatggac gtttcgatta tcggctgcgt ggtgaatggc ccaggtgagg
     6421 cgctggtttc tacactcggc gtcaccggcg gcaacaagaa aagcggcctc tatgaagatg
     6481 gcgtgcgcaa agaccgtctg gacaacaacg atatgatcga ccagctggaa gcacgcattc
     6541 gtgcgaaagc cagtcagctg gacgaagcgc gtcgaattga cgttcagcag gttggaaaaa
     6601 taataacgtg atgggaagcg cctcgcttcc cgtgtatgat tgaacccgca tggctcccga
     6661 aacattgagg gaagcgttga gggttcattt ttatattcag aaagagaata aacgtggcaa
     6721 aaaacattca agccattcgc ggcatgaacg attacctgcc tggcgaaacg gccatctggc
     6781 agcgcattga aggcacactg aaaaacgtgc tcggcagcta cggttacagt gaaatccgct
     6841 tgccgattgt agagcagacc ccgctattca aacgtgcgat tggtgaagtc accgacgtgg
     6901 ttgaaaaaga gatgtacacc tttgaggatc gcaatggcga cagcctgact ctgcgccctg
     6961 aagggacggc gggctgtgta cgcgccggca tcgagcatgg tcttctgtac aatcaggaac
     7021 agcgtctgtg gtatatcggg ccgatgttcc gtcacgagcg tccgcagaaa gggcgttatc
     7081 gtcagttcca tcagttgggc tgcgaagttt tcggtctgca aggtccggat atcgacgctg
     7141 aactgattat gctcactgcc cgctggtggc gtgcgctggg tatcttcgaa cacgtaactc
     7201 ttgagctgaa ttctatcggt tcgctggaag cacgcgccaa ttaccgcgat gcgctggtgg
     7261 cattccttga gcagcataaa gaaaagctgg acgaagactg caaacgccgc atgtacacta
     7321 acccgctgcg cgtgctggat tcaaaaaatc cggaagtgca ggcgcttctc aacgacgctc
     7381 cggcattagg tgattatctg gacgaggaat ctcgtgagca ttttgccggt ctgtgcaaac
     7441 tgctggagag cgcggggatc gcttacaccg taaaccagcg tctggtgcgt ggtctggatt
     7501 actataaccg taccgttttc gagtgggtga ctaacagtct cggctcccag ggcaccgtgt
     7561 gtgcaggcgg tcgttatgac ggtcttgtgg aacaactggg cggtcgtgca acaccggctg
     7621 tcggttttgc gatgggcctc gaacgtcttg tattgttagt acaggccgtt aatccggaat
     7681 ttaaagccga tcctgttgtc gatatatacc tggtggcttc aggtgctgat acacaatctg
     7741 cggctatggc attagctgag cgtctgcgtg atgaattact gggcgtgaaa ttgatgacca
     7801 accacggcgg cggcaacttt aagaaacagt ttgcccgtgc tgataaatgg ggtgcccgcg
     7861 ttgctgtggt gctgggtgag tctgaagtgg ctaacggcac agcagtagtg aaggatttgc
     7921 gctctggtga gcaaacggca gttgcgcagg atagcgtagc cgcgcatttg cgcacgttac
     7981 tgggttaagg aaggagaagg acagcgtgga aatttacgag aacgaaaacg accaggtaga
     8041 agcggttaaa cgcttttttg ctgaaaatgg caaagcactg gctgttgggg ggattttggg
     8101 cgttggcgca ctgactggct ggcgctactg gaacagccat caggttgatt ctgcacgctc
     8161 cgcttctctt gcctatcaaa atgcggttac cgcagtgagc gaaggcaaac cggatagcat
     8221 cccggcggcg gaaaaatttg ctgctgaaaa taaaaatact tatggtgcgc tggcttcttt
     8281 ggaacttgcg cagcaatttg ttgacaaaaa tgaactagag aaagctgccg cccagttaca
     8341 acaggggctg gcagacacga gcgatgaaaa tctcaaagcc gtgataaatc tgcgtcttgc
     8401 tcgcgttcag gtacagctca agcaggctga tgccgcgctg aaaacccttg ataccatcaa
     8461 aggtgaaggg tgggctgcca ttgttgccga cttgcgtggt gaagcattgc tgagcaaagg
     8521 tgataagcaa ggtgcgcgta gtgcatggga agcaggcgtg aaaagcgatg ttactccggc
     8581 actgagcgaa atgatgcaga tgaaaattaa taatttgtcc atctgagagg gacccgatgc
     8641 aattgcgtaa attactgctg ccaggactgc tttccgttac ccttttaagc ggctgttcgc
     8701 tgtttaacag cgaagaagat gtggtaaaga tgtccccatt gccaaccgtt gaaaaccagt
     8761 ttacgccgac cacggcgtgg agcacttccg ttggtagcgg cattggcaac ttctattcca
     8821 atcttcatcc ggcactggcg gacaacgttg tctatgcagc ggaccgcgct ggtttggtaa
     8881 aagcgctgaa tgcggatgat ggcaaagaaa tctggtctgt cagcctggcc gagaaagatg
     8941 gctggttctc taaagagcct gcattacttt ctggcggtgt gaccgtgtct ggtgggcatg
     9001 tctacattgg cagcgaaaag gcgcaggttt acgcgctgaa taccagcgat ggtactgtgg
     9061 catggcaaac taaagtcgcg ggtgaagcac tttcgcgccc ggtggtcagc gacggtctgg
     9121 tgttaatcca caccagtaac ggtcagttac aagcgctgaa cgaagctgac ggcgctgtca
     9181 aatggacagt taacctcgat atgccttcgc tctctttgcg tggcgagtct gcgccggcaa
     9241 cggcttttgg tgcggccgtc gtgggtggcg ataatggtcg cgtcagcgca gtgctgatgg
     9301 aacagggcca gatgatttgg cagcagcgta tttcccaggc gaccggttct accgaaattg
     9361 accgtctgag cgatgttgac acgactcccg tcgttgttaa cggcgttgtt ttcgcgctgg
     9421 cctataatgg taacctgacg gcgcttgatc tgcgcagtgg tcagattatg tggaaacgcg
     9481 aactgggttc ggtgaatgat ttcatcgtcg atggcaatcg catctatctg gtcgatcaaa
     9541 atgaccgggt gatggcgttg accattgatg gcggcgttac gctgtgggca caaagcgatc
     9601 tgctgcatcg cctgctgact tctccggtgc tgtataatgg caacctggtg gtcggtgaca
     9661 gtgaaggtta tctgcactgg attaacgtcg aagatggtcg tttcgttgcc cagcaaaaag
     9721 ttgatagttc cggtttccag actgaaccgg ttgccgctga cggcaaactg ctgatccagg
     9781 caaaagacgg aaccgtgtac tctattacac gttaatcgtc tctgtcgttc actttgaaaa
     9841 cggctcctgg acaggggccg ttttcctgtt tttaacaacg acgcgaatat agcgtgcgtt
     9901 gtctgatgat ttataaaaat gaggctttaa acatggtacc tgtggtcgcg cttgtcgggc
     9961 gccctaacgt aggaaaatcc acgttattta accgtctaac tcgcacccga gatgcgctgg
    10021 ttgcggattt cccgggtctg actcgtgacc gtaagtacgg tcgtgcggaa attgaaggcc
    10081 gtgagtttat ctgtattgat accggcggga ttgatggcac agaagacggt gtagaaaccc
    10141 gcatggcgga acagtcgctg ctggcgattg aagaagcgga cgtcgtactg tttatggtgg
    10201 atgcgcgcgc gggcctgatg ccggcagatg aagcgattgc caaacatctg cgctcccgtg
    10261 aaaaaccgac cttcctggtg gtaaacaaaa ctgacggtct ggatcccgat caggcagtgg
    10321 ttgatttcta ctcgcttggt ttaggtgaaa tctacccgat cgccgcgtct cacggtcgtg
    10381 gcgtattaag tctgctggag catgtgctgc tgccgtggat ggaagatctc gcaccgcaag
    10441 aggaagtcga cgaagacgct gaatactggg cgcaatttga agcggaagag aacggcgaag
    10501 aagaagagga agacgacttc gacccgcaaa gtctgccgat caaactggcg attgtgggtc
    10561 gtccgaacgt aggtaagtct acactcacta accgtattct tggagaagag cgcgttgttg
    10621 tttacgacat gcctggcacg acgcgtgaca gcatctacat cccaatggaa cgcgatggac
    10681 gtgagtatgt gctcattgac accgctggcg tacgtaaacg cggcaaaatc accgatgctg
    10741 tagagaaatt ctccgtaatc aaaacgttgc aggccattga agacgccaac gtggtgatgt
    10801 tagtgattga tgcgcgcgaa ggtatttccg atcaggatct ctcgctgctg ggctttattc
    10861 tcaatagtgg gcgctcactt gtcattgtgg tgaataagtg ggatggcctg agccaggaag
    10921 tgaaagagca ggtgaaagaa acgctggact tccgtctggg ctttatcgat tttgctcgtg
    10981 tgcactttat ctctgccttg cacggcagtg gtgttggtaa cttgtttgaa tcagtacgtg
    11041 aagcgtatga cagctccacc cgtcgtgtgg ggacctctat gctgacgcgc atcatgacga
    11101 tggctgttga agatcaccaa ccgccgctgg tacgcggtcg tcgtgtgaag ctgaaatatg
    11161 cccacgctgg tggttataac ccgccgattg tggtgattca cggtaatcag gtgaaagacc
    11221 tgcctgattc ctacaagcgc tacttgatga actacttccg caaatcgctg gacgtaatgg
    11281 gatcgccgat tcgtattcag ttcaaagaag gggaaaaccc gtatgcgaac aagcgtaaca
    11341 ccctgacgcc aacccagatg cgtaaacgta agcgtctgat gaagcacatc aagaaaaata
    11401 aataattatc cgtaagtatt ttatctgcca ttccctctat gaatgtagag ggaatggcta
    11461 ccggaggaat tcatatggaa ctgcattgtc caaaatgtca gcatgtgctt gatcaggata
    11521 acggccatgc gcgttgccca tcctgcgggg aatttattga aatgaaagct ctttgtccag
    11581 actgccatca accgttgcag gtgttaaaag cctgcggtgc agtagattac ttctgtcagc
    11641 acgggcatgg cttgatatcg aaaaaacgcg ttgaattcgt gctggcttaa tgaacctttt
    11701 tacgcgattt ttttaccggc tggatgtttt ttacttcact ttctatccag ccgtcttcca
    11761 gacgtgtggt tagcatttca cccgttttaa cttgcttcac ttttttcagt acattgccgt
    11821 cagtagcagt agtaacgctg tatccacgcg ccagcgttga cagtgggctt acggcttcga
    11881 ggtgcgttac tgcattaccg aaacgttcac gcgtggcgct aagctgtgcg cgcaggattt
    11941 ctgctaaacg atattccagt tgctgaatgc gcgtttgcgt gcgatgaatc ttcggctgtg
    12001 gattttgctg attcagccgc tgtgttaatc gctgctgctg ttgcccggca cgcttaagct
    12061 gattttccag cgcaaagctc atccgttttt gcaggcgttc aagcatggtt tgctggcgtg
    12121 ccagccggag ctgcggatgc tgctgctgca agcgatgatg aatctgcgta aagcgacgtg
    12181 tacggttggc gagataataa tccatcgcca tctccagccg ttgacgggcc gattgcacct
    12241 ggcgcagtaa ctcttgctga ttacggctca ctacttcagc ggcggcagac ggcgttggcg
    12301 cacgcagatc ggcaacaaaa tcggcaatgg tcacatccgt ctcatgcccg acggcgctga
    12361 cgaccggaat gcggctggca aaaatcgccc gcgctacgcg ttcgtcgtta aaactccata
    12421 aatcttccag cgaaccgccg ccgcgcccaa cgatcaacac gtcgcactca ttgcgctgat
    12481 tcgccagttc aatggcgcga acaatttgcc ccggcgcgtc atcgccctga acggaggtgg
    12541 ggtagatgat caccggtaga gaaggatcgc gacgttttaa cacatgcaaa atatcatgta
    12601 gcgcagcacc ggtttttgag gtgatcacac caacgcaatg cgcaggggag ggaagtggtt
    12661 ttttgtattg ctgatcgaac aaacattcag cctgcaactt cgctttgagc tgttcgtact
    12721 tctgttgcag cagcccttca ccggccggct gcatactctc aacgattatc tggtagtcgc
    12781 cgcgcggctc gtagagcgta atattggcgc gaactaaaac ttgttgccca tgctgcggac
    12841 ggaaggtgac ccggcggttg ctgttgcgga acatcgcgca gcgtacctgg gcggtgtcgt
    12901 ctttgagtgt aaagtaccag tgaccggaag ccggttgtgt gaaattagag atttcgccgc
    12961 tgatccaaac ctgtcccatc tcatgctcaa gcagcagacg aaccgtttga ttcaggcgac
    13021 taacggtaaa aattgcaggg gattgagaag gtaacatgtg agcgagatca aattctaaat
    13081 cagcaggtta ttcagtctat agtaacccgc ccttcgggga tagcaagcat tttttgcaaa
    13141 aaggggtaga tgcaatcggt tacgctctgt ataatgccgc ggcaatattt attaaccact
    13201 ctggtcgaga tattgcccat gctacgtatc gctaaagaag ctctgacgtt tgacgacgtt
    13261 ctcctcgttc ctgctcattc taccgttctg ccgaatactg ctgacctcag cacccagctg
    13321 acgaaaacta ttcgtctgaa tatccctatg ctttccgcag caatggatac cgtaacggaa
    13381 gcgcgcctgg ctattgctct ggctcaggaa ggcggtatcg gctttatcca caaaaacatg
    13441 tccattgaac gccaggcaga agaagttcgc cgtgtgaaaa aacacgaatc tggtgtggtg
    13501 actgatccgc agactgttct gccaaccacg acgctgcgcg aagtgaaaga actgaccgag
    13561 cgtaacggtt ttgcgggcta tccggtcgtt accgaagaaa acgaactggt gggtattatc
    13621 accggtcgtg acgtgcgttt tgttaccgac ctgaaccagc cggttagcgt ttacatgacg
    13681 ccgaaagagc gtctggtcac cgtgcgtgaa ggtgaagccc gtgaagtggt gctggcaaaa
    13741 atgcacgaaa aacgcgttga aaaagcgctg gtggttgatg acgaattcca cctgatcggc
    13801 atgatcaccg tgaaagactt ccagaaagcg gaacgtaaac cgaacgcttg taaagacgag
    13861 caaggccgtc tgcgtgttgg tgctgcagtt ggcgcaggtg cgggtaacga agagcgtgtt
    13921 gacgcgctgg ttgccgctgg cgttgacgtt ctgctgatcg actcctccca cggtcactca
    13981 gaaggcgttt tgcagcgtat ccgtgaaacc cgtgctaaat atccggatct gcaaatcatc
    14041 ggcggcaacg tggcaacagc tgcaggtgca cgcgctttgg cagaagctgg ttgcagcgcg
    14101 gttaaagtcg gtatcggccc tggttctatc tgtacgactc gtatcgttac tggcgtaggt
    14161 gttccgcaga tcactgccgt tgctgacgca gttgaagccc tggaaggcac cggtattccg
    14221 gttatcgcag atggtggtat tcgcttctcc ggcgacatcg ccaaagctat cgccgctggc
    14281 gcaagcgcgg tgatggtagg ttccatgctg gcgggtactg aagaatctcc gggtgaaatc
    14341 gaactctacc agggccgttc ttataaatct taccgtggta tgggttccct gggcgcgatg
    14401 tccaaaggtt cctctgaccg ttatttccag agcgataacg ctgccgacaa actggtgccg
    14461 gaaggtatcg aaggtcgcgt agcttataaa ggtcgcctga aagagatcat tcaccagcag
    14521 atgggcggcc tgcgctcctg tatgggtctg accggctgtg gtactatcga cgaactgcgt
    14581 actaaagcgg agtttgtacg tatcagcggt gcgggcattc aggaaagcca cgttcacgac
    14641 gtgaccatta ctaaagagtc cccgaactac cgtctgggct cctgattctc ttcgcccgat
    14701 ttcatgtcgg gcgatttata ttatctgttt cacttgcctc ggaataagcg tcaatgacgg
    14761 aaaacattca taagcatcgc atcctcattc tggacttcgg ttctcagtac actcaactgg
    14821 ttgcgcgccg cgtgcgtgag ctgggtgttt actgcgaact gtgggcgtgg gatgtgacag
    14881 aagcacaaat tcgtgacttc aatccaagcg gaattattct ttccggcggc ccggaaagta
    14941 ctactgaaga aaacagtccg cgtgcgccgc agtatgtctt tgaagcaggc gtaccgatat
    15001 tcggcgtttg ctatggcatg cagaccatgg caatgcagtt gggcggtcac gttgaagcct
    15061 ctaacgaacg tgaatttggc tacgcgcagg tagaagtcgt aaacgacagc gcactggttc
    15121 gcggtatcga agatgcgctg accgcagacg gtaaaccgct gctcgatgtc tggatgagcc
    15181 acggcgataa agttaccgct atcccgtccg acttcgtcac cgtagccagc accgaaagct
    15241 gcccgtttgc cattatggct aacgaagaaa aacgcttcta tggcgtacag ttccacccgg
    15301 aagtgactca cacccgccag ggtatgcgca tgctggagcg ttttgtgcgt gatatctgcc
    15361 agtgtgaagc cctgtggacg ccagcgaaaa ttatcgacga tgctgtagct cgcatccgcg
    15421 agcaggtagg cgacgataaa gtcatcctcg gcctctctgg tggtgtggat tcctccgtaa
    15481 ccgcaatgct gctgcaccgt gctatcggta aaaacctgac ttgcgtattc gtcgacaacg
    15541 gcctgctgcg tctcaacgaa gcagagcagg ttctggatat gtttggcgat cactttggtc
    15601 ttaacattgt tcacgtaccg gcagaagatc gcttcctgtc agcgctggct ggcgaaaacg
    15661 atccggaagc aaaacgtaaa atcatcgggc gcgttttcgt tgaagtattc gatgaagaag
    15721 cgctgaaact ggaagacgtg aagtggctgg cgcagggcac catctaccct gacgttatcg
    15781 aatctgcggc ttctgcaacc ggtaaagcac acgtcatcaa atctcaccac aacgtgggcg
    15841 gcctgccgaa agagatgaag atgggcctgg ttgaaccgct gaaagagctg ttcaaagacg
    15901 aagtgcgtaa gattggtctg gagctgggcc tgccgtacga catgctgtac cgtcatccgt
    15961 tcccgggacc aggccttggc gttcgtgttc tgggtgaagt gaagaaagag tactgtgacc
    16021 tgctgcgccg tgctgacgcc atcttcattg aagaactgcg taaagcggac ctgtacgaca
    16081 aagtcagcca ggcgttcacc gtcttcctgc cagtacgttc cgttggcgta atgggcgatg
    16141 gtcgtaagta tgactgggtt gtctctctgc gtgctgtcga aaccatcgac tttatgaccg
    16201 cacactgggc gcatctgccg tacgatttcc tcggtcgcgt ttccaaccgc attatcaatg
    16261 aagtgaacgg tatttcccgc gtggtgtatg acatcagcgg caagccgcca gctaccattg
    16321 agtgggaatg attttgatcc ctgcactatg aatgaacaaa accctccgtt actacagagg
    16381 gttttttatc ttcaagaatc ataggcttga agttactaac atcgattaat taaaccagct
    16441 gtccgatttg ttctcttctg ctttgcccac gcttttcatc agatcgcgac cgccttcagt
    16501 catatttctg tttgcgtcag cttcagattg caccacatcg gtttgcgcag ctttgtgctt
    16561 cagttcctga tcgataaatt cgttttcgcg cttaacgcgg gcttcttctt tcgccagcgc
    16621 cagtttttgt ttctgaatct ctaagctgcg tagctcatct tcataacttt gatcgcgttt
    16681 tttgtccgca gtggcttcgg cgtccagttt atcctgacga gctttcttat ttgccgctgc
    16741 cgttgccgct cttttgttcg cggcggcctg ggcatttgcg cgacgttgct tctcttgctg
    16801 gatttccctg ttgcgctccg cgacccattc gtcatgctgc ctttgctctt catttttacc
    16861 ttgctgttcc gcttctgcga cagcagagag ttgatcctgc aatgatgagg ctatagccgg
    16921 atagcttaag gaggctaaga tggcgcaaag aaaaactttc ttcatgactc ctcctgatta
    16981 ttagctcttt tcaggacatt tggtatttgg ctgaatacgc gtttcgttat acgttgtggt
    17041 aataacaacg gctaaacctg tcgtaaactg gcactcttta ccctcctggg tggaggtata
    17101 cactttggtg ccttccttat atgttaaaga aacaccttcc actaaggttt tatcattcac
    17161 catagaaccc gctgccgcgc ctacagctcc gccgccaact gcccctgccg tcgttccgga
    17221 attgctgcca gaaccgacgt tgtggccgat aacaccgcca gcgactgcgc caataagcgc
    17281 gccgaaggct tgtgcgttcc gtttattttg ggagttgtct acggcaactt ttgcgggaag
    17341 aatggaaata atattaacgg ttttagtttc ttgtttggta ttcagttgat cggtttgata
    17401 aacatcggcg gcatgatcgt cagcatttga ctggcatcct gccagagtga atgacgctaa
    17461 cattgccaca ggcagaagac attttttaaa tttcatcact attccttgtt atacacatta
    17521 cgaaaaataa gacatttaag atcatcaaaa gaggaaagta aatgtattgc gtataaaggg
    17581 atagattaaa tttattaaaa ttcacgcagg gaataattta taaaaatata tcattacaac
    17641 atatatatta attttatatt tatttcgcag ccaaacagga taaacatcag tcaggacaaa
    17701 gacgtaaaat ttaagatgaa agaatatctg ccgctttgat aatcccaaag agcaaaagca
    17761 tccgacagcg aagtgccgtt atgttctgtg aaagtggaga aggtaaccgc taacgttgta
    17821 ccggtgattc gacggagagt gtggattgct gagcttcttt tttgctctga tggtgctgcc
    17881 aggcaccgac ggacgagtag ataaaacggc caaagaagaa gataaagctg atgagcagta
    17941 cgatacgggt catgcgactg ttaaatcggt gtcgttttcg catactagtt gcctgactca
    18001 caaaaggttc cttgaagtat gtcccacgct gtggacggta cttacattaa ggcacaacag
    18061 gacaaaatgg tcaattcttc gttatgtaaa aaagcgtcag tggatacata ttttaatgtt
    18121 atggaagtta atttaattat ttacaatgat gatgtaataa tgatgaatta acatgataaa
    18181 cgtaataatt cattaatcag ggttattttg tttgatatat atcaattgga actttcatat
    18241 gacacttaga atcaatgctc tctatagtga tgaataatca tcattcgaag tcaggtggga
    18301 tgcctgtctg aatacacctc cttcaggatg tgggggattc tgctgagcat ctatgaaact
    18361 gaatgcaact tatataaaaa tacgtgataa atggtggggg cttccgctgt tcctgccttc
    18421 tttaatcttg cccattttcg cccacattaa tactttcgcg catatttctt ccggtgaggt
    18481 ttttctcttt tatctgcctt tggcactgat gatcagcatg atgatgtttt tcagctgggc
    18541 ggcattgcca gggatcacct tagggatttt tgtccgcaaa tatgcagagc tgggttttta
    18601 cgaaacgctc tcattaacgg ctaattttat tatcattatc attctctgtt ggggcggtta
    18661 cagggtcttt actccccggc gtaacaacgt ttcacatggt gatacccgtt taatttccca
    18721 gcgtatattc tggcagatag tgtttcctgc aacgctgttt ctgatacttt tccagtttgc
    18781 tgcatttgta ggattactgg cgagcagaga aaatctggtc ggtgtcatgc cttttaacct
    18841 cgggacttta atcaattatc aggccttact ggtgggtaat ctgatcggtg tcccgctgtg
    18901 ctacttcatc attcgggtag tgcgaaatcc attttattta cgtagctatt attcgcaatt
    18961 aaaacagcag gttgatgcca aagtcaccaa aaaagagttc gcgctctggc tactggcatt
    19021 aggtgcttta ttgttgctgt tatgcatgcc gttaaatgaa aaaagcacaa tttttagcac
    19081 caattatacc ttgtcattat tgctgcccct gatgatgtgg ggagcaatgc gctatggtta
    19141 taagctgatt tcgctgctct gggcggtcgt gttgatgatc agcatccaca gctatcaaaa
    19201 ttacattccc atttatcctg gctataccac gcagctgacc ataacctcct ccagttatct
    19261 ggtattctct tttattgtca attatatggc tgtactggca actcgtcagc gagcggtagt
    19321 cagacgcatt cagcggcttg cgtatgtgga cccggtggtt catctgccaa atgttcgcgc
    19381 cctgaatcgc gcgttgcgtg atgccccctg gtctgcgctt tgttatttac gcatccctgg
    19441 catggaaatg ctggttaaga actatggcat catgctgcgg attcaa
//
LOCUS       DAJTSF010000079        18068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000079 DAJTSF010000000
VERSION     DAJTSF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.079"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            256..780
                     /locus_tag="OO335_002872"
     CDS             256..780
                     /locus_tag="OO335_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6003189.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            861..1535
                     /locus_tag="OO335_002873"
     CDS             861..1535
                     /locus_tag="OO335_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6003190.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1551..4013
                     /locus_tag="OO335_002874"
     CDS             1551..4013
                     /locus_tag="OO335_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6003191.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            4029..5062
                     /locus_tag="OO335_002875"
                     /pseudo
     CDS             4029..5062
                     /locus_tag="OO335_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5144..5482)
                     /gene="rcnB"
                     /locus_tag="OO335_002876"
     CDS             complement(5144..5482)
                     /gene="rcnB"
                     /locus_tag="OO335_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR6003192.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5701..6531)
                     /gene="rcnA"
                     /locus_tag="OO335_002877"
                     /pseudo
     CDS             complement(5701..6531)
                     /gene="rcnA"
                     /locus_tag="OO335_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6653..6925
                     /gene="rcnR"
                     /locus_tag="OO335_002878"
     CDS             6653..6925
                     /gene="rcnR"
                     /locus_tag="OO335_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR6003193.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      7017..7113
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7148..7936
                     /gene="thiM"
                     /locus_tag="OO335_002879"
     CDS             7148..7936
                     /gene="thiM"
                     /locus_tag="OO335_002879"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6003194.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7933..8733
                     /gene="thiD"
                     /locus_tag="OO335_002880"
     CDS             7933..8733
                     /gene="thiD"
                     /locus_tag="OO335_002880"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6003195.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8798..9619
                     /locus_tag="OO335_002881"
     CDS             8798..9619
                     /locus_tag="OO335_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6003196.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9667..10413
                     /locus_tag="OO335_002882"
     CDS             9667..10413
                     /locus_tag="OO335_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6003197.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10387..11352)
                     /locus_tag="OO335_002883"
     CDS             complement(10387..11352)
                     /locus_tag="OO335_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR6003198.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11349..12352)
                     /locus_tag="OO335_002884"
                     /pseudo
     CDS             complement(11349..12352)
                     /locus_tag="OO335_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12392..13444
                     /gene="fbaB"
                     /locus_tag="OO335_002885"
     CDS             12392..13444
                     /gene="fbaB"
                     /locus_tag="OO335_002885"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6003199.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13752..14606
                     /gene="gatY"
                     /locus_tag="OO335_002886"
     CDS             13752..14606
                     /gene="gatY"
                     /locus_tag="OO335_002886"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6003200.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14635..15897
                     /gene="gatZ"
                     /locus_tag="OO335_002887"
     CDS             14635..15897
                     /gene="gatZ"
                     /locus_tag="OO335_002887"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR6003201.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15907..16359
                     /gene="gatA"
                     /locus_tag="OO335_002888"
     CDS             15907..16359
                     /gene="gatA"
                     /locus_tag="OO335_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6003202.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16390..16674
                     /gene="gatB"
                     /locus_tag="OO335_002889"
     CDS             16390..16674
                     /gene="gatB"
                     /locus_tag="OO335_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6003203.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16678..18033
                     /locus_tag="OO335_002890"
     CDS             16678..18033
                     /locus_tag="OO335_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR6003204.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4727 a         4112 c         4570 g         4659 t
ORIGIN      
        1 aatcatttat ataaatgatt tcgtcttttt tattgatatc aacaatacca tttacataaa
       61 tctacattaa ttaatagatg tgataaatat ttaatattct taagtatttg ttgtatttat
      121 attgcattgg ttaatttgtt ttaaatcaat aaaacaatat gtttacattg cggcagcttt
      181 tttgatgtga gaaatttgtt tccaagagat acagactctt aacaaccaaa aataaatttt
      241 attaaggaac atattatgaa acgttcaatt attgctgccg ctgtcttttc ttcttttttt
      301 atgagcgctg gagtatttgc tgcagacgtt gataccggaa cattaaccat taaaggaaat
      361 attgcagaat ctccgtgtaa attcgaagcg ggtggtgatt cagtaagtat taatatgccg
      421 actgtaccaa ccactgtctt tgaaggtaaa gctaaatatt ctacctatga tgatgcagtc
      481 ggtgtaacca gcagcatgtt aaaaattagc tgcccgaaag aagttgctgg tgtaaaactc
      541 tcgttgatca caaacgacaa aataaccggt aacgataagg cgatagccag tagcaacgat
      601 accgtaggtg ataacagcga tgtcctagat gtttctgcac cttttaacat tgagagttat
      661 aaaacagcgg aaggtcaata tgctattccg tttaaagcaa aatacctgaa actgacagat
      721 aactcagtgc aatcaggtga tgtgttatct tctctggtta tgcgtgtggc gcaggattaa
      781 taaataaaat tgcaagtttc tccaggtaat aaacatggcc gctatcccca tggcggcttt
      841 ttaatttaag aggcattaag atgaaaggat tattatcttt actcattttt tctatggtcc
      901 ttcctgcaca tgccggaatt gttatctacg ggacgcgcat tatttacccg gcagaaaata
      961 aagaagttat ggtgcagttg atgaaccagg gaaatcgttc ttcgctgctg caggcgtgga
     1021 ttgatgatgg cgatacgtcg ttaccaccag aaaaaattca ggttcctttc atgttaacgc
     1081 caccagtggc aaaaataggg gcaaattccg ggcagcaagt aaaaatcaaa attatgccga
     1141 ataaactgcc cactaataaa gaaagcattt tttatctgaa tgttctgtat attccaccaa
     1201 atagtccaga gcaagaaggc aagaatgcac tgaagtttgc gatgcaaaac agaattaagt
     1261 tgttttaccg gccagcgggt attgctccgg taaataaagc gacctttaaa aagttactgg
     1321 taaatcgcag tggcaatgga ttggtgataa aaaatgactc agctaattgg gtgacgattt
     1381 cggatgtcaa agctaataat gttaaagtca attatgaaac tattatgatt gcccccttag
     1441 aaagtcagag tgttaatatc aaaagtaata atgcaaataa ctggtatctg accattatca
     1501 atgaccacgg caactatatt agtgacaaaa tttaattgca ggagctgcct atgttgagaa
     1561 tgaccccact tgcatcagca atcgtagcgt tattgctcgg cattgaagct tatgcagctg
     1621 aagaaacctt tgatacccat tttatgatag gtggaatgaa agaccagcag gttgcaaata
     1681 ttcgtcttga tgataatcaa cccttaccgg ggcagtatga catcgatatt tatgtcaata
     1741 agcaatggcg cgggaaatat gagattattg ttaaagacaa cccgcaagaa acatgtttat
     1801 caagagaagt tatcaagcgg ttaggcatta atagcgataa cttcgccagc ggtaagcaat
     1861 gtttaacatt tgagcaactt gttcagggtg ggagctatag ctgggatatc ggggtttttc
     1921 gtctcgattt cagtgtcccg caggcttggg tggaagaact ggaaagtggc tatgttccac
     1981 cggaaaactg ggagcggggt attaatgcgt tttatacttc ttattatgtg agtcagtatt
     2041 acagcgacta taaagcgtcg ggtaacaaca agagtacata tgtacgtttt aacagcgggt
     2101 taaatttact ggagtggcaa ctgcattctg atgccagttt cagtaaaaca aataacaatc
     2161 caggggtgtg gaaaagcaat accctgtatc tggaacgtgg atttgcccaa tttctcggca
     2221 cgcttcgcgt gggtgatatg tacacatcaa gcgatatttt tgattctgtt cgcttcagcg
     2281 gtgtgcggtt gtttcgtgat atgcagatgt tgcctaactc gaaacaaaat tttacgccac
     2341 gggtgcaggg gattgctcag agtaacgcgc tggtaactat tgaacagaat ggttttgtgg
     2401 tttatcagaa agaggttcct cctggcccgt ttgcgattac agatttgcag ttggccggtg
     2461 gtggagcaga tcttgatgtt agcgtgaaag aggcggacgg ctcggtaacc acctatctgg
     2521 tgccttatgc agcggtgcca aatatgctgc aacccggcgt gtcgaaatat gattttgcgg
     2581 cgggggcgag caagcaaagt gattttgtcc aggcaggtta tcagtatggt tttaataact
     2641 tattgacgct gtatggcggc acgatggttg ctaataatta ctatgcgttt accctcggaa
     2701 cgggttggaa cacgcgcatt ggcgcaattt ccgtcgatgc cacgaagtcg catagtaaac
     2761 aagacaacgg cgatgtgttt gacgggcaaa gttatcaaat tgcctacaac aaatttgtga
     2821 gccaaacgtc gacgcgtttt ggtctggcgg cctggcgtta ttcgtcgcgt gattaccgga
     2881 catttaacga tcacgtttgg gcaaacaata aagataatta tcgccgtgat gaaaacgata
     2941 tctatgacat tgccgattat taccagaacg attttggccg taaaaatagc ttttctgcca
     3001 atatgagcca gtcattgcca gaaggctggg gttctgtgtc cttaagtacg ttatggcgag
     3061 attactgggg gcgtagcggc agcagtaagg attatcagtt gagttattcc aataactggc
     3121 ggcggataag ctataccctc gcggcaagcc aggcgtatgg cgagaatcat catgaagaga
     3181 aacgttttaa tatttttata tcaattccct gtgattgggg tgatgacgtt acgacgcctc
     3241 gtcggcaaat atatatgtct aactcaacga cgtttgatga tcagggtttt gcctcaaata
     3301 atacgggatt atcaggaaca gtagggagtc gggatcagtt caattatggt gtcaacctga
     3361 gtcatcaaca tcagggaaat gaaacgacag ctggggcgaa tttgacctgg aacgcgccgg
     3421 ttgcgacagt gaatggcagt tatagtcagt cgagtactta tcgacagact ggagccagtg
     3481 tttcaggggg cattgtcgcc tggtcgggtg gcgttaatct ggcgaaccgt ctttccgaaa
     3541 cgtttgctgt gatgaatgcg ccaggaatta aagatgctta tgtcaatggg caaaaatatc
     3601 gcacaacaaa ccgtaatgga gtggtggtat acgacggaat gacaccttat cgggaaaatc
     3661 acctgatgct ggatgtgtcg caaagcgata gcgaagcaga attacgtggc aaccggaaaa
     3721 ttgccgcccc ttatcgcggc gcggttgtac tggttaattt tgataccgat cagcgcaagc
     3781 cctggtttat aaaagcgtta agagcggatg gtcaaccatt aacgtttggt tatgaagtca
     3841 atgatatcca tggtcataat attggcgttg tcggccaggg aagccagtta tttattcgca
     3901 ccaatgaaat accgccatcg gttaatgtag caattgataa gcaacaagga ctttcatgca
     3961 caatcacctt cggtaaagag attgatgaaa gtagaaatta tatttgccag taatagtcag
     4021 gtggttctat ggaaattcgc ataatgctat ttatattaat gatgatggtt atgcctgtga
     4081 gctatgcggc atgttatagt gagttatctg ttcagcacaa tttggttgtt caggggaatt
     4141 ttgcacttac tcaaacacaa atagcgacat atgagcataa ttttaatgat tcgtcatgcg
     4201 tgagtacaaa tactatcacc cctatgagcc catcggatat tattgttgga ctttataacg
     4261 ataccattaa attaaattta cattttgaat ggaccaataa aaacaacatc acgttgtcaa
     4321 ataatcagag cagtttcacc agtggttatt cggtaacggt gacacctgcg gccagtaatg
     4381 caaaagtgaa tatttctgcg gggggcggtg gttcagtgat gattaatggt gttgcgacat
     4441 tatccagtgc ttcatcatcg acacgcggga gtgccgcagt acaatttcta ctgtgtttat
     4501 taggtggcaa gtcatggggt gcatgtgtaa atagctacag aaatgcattg gcacaaaatg
     4561 caggtgtcta ttccttcaat ctgacattgt catacaaccc gataaccaca acctgcaaac
     4621 aggacgattt attaattact ttagacagta ttcccgtttt acaattacca gccacaggta
     4681 acaaagcaac aataaatagt aaaaaaaggg atattattct gcgttgtaaa aatttattag
     4741 gtcaacaaaa tcaaacatca cggaaaatgc aggtgtattt atcaagttct gacttgttaa
     4801 ccaacagcaa cacaatactg aaaggtgcgg aagataatgc gtaggattta ttcttgaaag
     4861 taatggttcg ccagtcacac ttttaaatat cactaacagc agtaaaggat acacaaattt
     4921 aaaagaaatt gcggcaaagt caaaacttac agatacaacg gtttcaattc cgataacagc
     4981 cagttactac gtctacgata caaacaaagt taaatctggc gcactggagg caaccgcatt
     5041 aatcaacgtg aaatacgact aataacaagc aaaacgagca agtggctaat aataaaaaat
     5101 aacaaggtgt ctgcatttcc ccctcatgat gagggggctt tttttagcga tgataaaaaa
     5161 tctcaccgtc gtaggcttta atgattttac cgtcggtgtc gctgatcaac acgtacgcgc
     5221 cacccatata ggtccagtgc gtcccggcat caggcgcggg caggttacgt aactgccact
     5281 gcttaatgtt gtactcatcg gtgcggtaca tctctgggac ggtatcacct ggtttgaaat
     5341 gcttaaagtc agcaatgaaa ctacttaact catacttaga aattcccgag gtattggtcg
     5401 aacccgcggt ggctggtgcg gcccaggcgg cactggtaac cagcaaaagc acacccagca
     5461 acatcttatt tttaatggtc ataaactctc cactttgggg atctgtaata tcagtcgcat
     5521 atctttcctg tcatacaata taactgacaa gcgatatttt gttagtgatc ccatgatttc
     5581 gtaaaaagtg taaatgaaag gatgccctaa aagcatcctt agcgagcaga gagtcagcgg
     5641 aaatagctga tttaagaatc atcctgggct gaagataaca tgcaagccta aaggattgag
     5701 ttatcgcatt atgcccatga agccatgtac gcccatatac acaccgacta agccaatcaa
     5761 cagactggaa aaataggggg cgcgtttagc gagagtgtta aatccgctcc agcgttttgc
     5821 gacctgctga acgctgattg ctgcgccaac gcctacggtg acaagcgtta acgccaggcc
     5881 aatgctgaaa ctgacgacca gtgttgcgcc cagtgtcagg gctttcaact gaatgcatat
     5941 caacagcacg gtaattgctg ccgggcaggg gataaagcca ccggttaagc caaataacaa
     6001 aatttgccag ttgatgacct ctctaccatc aaagcgtcgt ttaatgtcat tggcatgggc
     6061 tcgtgcatgg gcatcctgat actcgccatg ttcgtgatgg tgatgatgtc cttggtcgtg
     6121 gtgatgttcg tgatcgtgat gatggtgatg atgctcataa tcatgcccgt gcatattctc
     6181 cagccagttg cgttcgccgc gctaggtacg ccagaacatc cagaacgcgg tgccaataat
     6241 gatcactgcg gaaatcagct ggagccacgg ttctgctgat tgagcagtaa agcgcttact
     6301 gatcaccatc ccgccaaagg caatcaacca gaccaccgcg gtatgcgaaa tggttgctgc
     6361 cagtccgagc atcaccgcct gtttaatggt gcctttgatg gcgatgataa acgccgccat
     6421 catcgttttt gagtgccctg gttccaggcc atgaagcgca ccaagtaaga tggcgctggg
     6481 gatgaagaac caggcgtttc cttgctgaag aagagttgta aattcggtca tgataataat
     6541 tcttagtatt aattcggcaa tctgattcta ctccccccca gtacctgata ctaccccccc
     6601 agtagattaa tagtgctatg atttttcata ttcttgttaa ccaggtgttg ccatgtctca
     6661 tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg gccaggtcgt
     6721 ggcgctcaag aaaatgctcg acgagccgca cgaatgcgct gcagttttac aacagattgc
     6781 tgctatccgt ggagcggtaa acggtctgat gcgggaagtg attaaaggtc atctgacgga
     6841 acacatcgtt caccaggggg atgagctaaa acgtgaagaa gatctggatg tcgttctgaa
     6901 ggtgctggat tcatatatca aataatttat taacgcgatt gtaaaactgc cgtttttcct
     6961 cgtttacaac gggggcgctg gacattacca tcctcctctg cgatttatca tcgcaaccaa
     7021 acgactcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc tgatctggat
     7081 aatgccagcg tagggaagtc acggaccacc aggtcattgc ttcttcacgt tatggcagga
     7141 gcaaactatg caagtcgacc tgctgagttc agcgcaatct gcgcacgcgt tacacctttt
     7201 tcaccaacat tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa cctttaccgc
     7261 caataccttg ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg aagaggccag
     7321 tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt ggcacactga cgcagccacg
     7381 cgcacaggcg atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac gctggacgct
     7441 tgatccagta gcggttggtg cgctcgatta tcgccgccat ttttgtcatg aacttttatc
     7501 ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc atggcattag ctggcattgc
     7561 taatggcgga cggggagtgg ataccactga cgccgcagct aacgcgatac ccgctgcaca
     7621 aacactggca cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg attatgttac
     7681 cgatggacat cgtatcattg gtattcacgg tggcgatccg ttaatgacca aagtggtagg
     7741 aactggctgt gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag gcgatacgct
     7801 ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc ggagaacgcg cagtcgccag
     7861 aagcgagggg ccaggcagtt ttgttccaca tttccttgat gcgctctggc aattgacgca
     7921 ggaggtgcag gcatgaaacg aattaacgct ctgacgattg ccggtactga tccgagtggt
     7981 ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac ttggcgctta tggttgctca
     8041 gttattactg cactggtggc gcaaaatacc cgtggcgtac agtcggtgta tcgcattgag
     8101 cctgattttg tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat cgataccact
     8161 aaaatcggta tgttggcgga aaccgatatt gttgaagcgg tggcagaacg gttgcaacgt
     8221 tatcagatcc aaaacgtggt actcgacacc gttatgctgg caaaaagcgg cgacccgctg
     8281 ctttcacctt cggcggttgc tacgctgcgc agtcgattat tgccacaggt ttcattaata
     8341 acgccaaact tgcccgaagc tgccgccttg ctcgacgcgc cacacgcgcg caccgaacag
     8401 gaaatgctgg aacaagggcg atcgctgttg gcgatgggct gtggcgcagt gctaatgaaa
     8461 ggtggtcatc tggatgatga gcaaagcccg gactggctgt ttacccgcga gggtgaacaa
     8521 cggtttaccg caccgcgcat tatgaccaaa aacacccacg gcactggttg tacactctct
     8581 gcggcgttgg ctgcactacg cccgcgccat acaaactggg ctgacaccgt acaggaggca
     8641 aaaatctggc tttcatcggc gttagcccag gccgacacgc tggaagttgg tcacggtatt
     8701 ggtccggttc accacttcca cgcctggtgg tgagcaccgt actatagctg ctttggttat
     8761 tgtcagcctt tggcactgac attcagggat gaaaccaatg cagctaagaa ttaccagtcg
     8821 aaaaaaactc accgctctat tatgcgctct tgggctaatt tccatcgttg caatttatcc
     8881 gcgtcagacg gtgaattttt tctattcgac agcagttcag attacagact acatccactt
     8941 ctacggttat cgcccggtta aatctttcgc tattcgcatt cctgccagtt acaccattca
     9001 cggattagat gtttcgcgct ggcaggagcg tatcgactgg cagcgggtgg caaaaatgcg
     9061 cgacaacgat atccgcttac agtttgcttt tattaaggct acggaaggcg aaaagctggt
     9121 ggacccctat ttttcgcgta actggcaatt aagccgcgaa aatggcctgc tgcgcggggc
     9181 gtatcattat ttctccccgt cggtatccgc ttcagttcag gcgcgattat ttctgcaaac
     9241 ggtggatttc tcacaaggcg atttccctgc cgtgctggac gtagaagaac ggggaaaatt
     9301 atcggcaaaa gaattacgca agcgggtaag tcagtggcta aaaatggtcg aaaaacgcac
     9361 gggaaaaaag ccgattattt actcaggagc cgttttttat cacaccaatc tggcgggcta
     9421 tttcaatgaa tatccgtggt gggtggctca ctattatcaa cgtcgtccgg acaatgacgg
     9481 catggcctgg cgcttctggc agcattccga ccgtggacag gtagatggca tcaatggtcc
     9541 ggtggatttt aatgtgttta atggcacggg gatgagttgc aggcattcgt tgatgggatt
     9601 aaagaaacgc cttaaataag aagcagtgat atactcccac cagactccct ggcaggaaaa
     9661 agacaaatgg aacaagcgca tacccagctg attgcccaac tgaacgagcg tattttagcg
     9721 gcggataaca cgccgcttta tattaagttt gccgaaacgg taaaaaatgc cgtgcgcagc
     9781 ggggtgctgg agcatggcaa tattttgccc ggtgagcgtg atttaagtca gttaaccggc
     9841 gtttcgcgca ttacggtgcg caaggcgatg caggcgctgg aagaagaggg cgtggtgacg
     9901 cgttcgcgcg gttacggcac gcagatcaac aacatcttcg aatactcgtt gaaagaagcg
     9961 agaggttttt ctcagcaggt ggtattgcgc gggaaaaagc ccgatacgct atgggttaat
    10021 aagcgtgtcg tgaaatgtcc cgaagaagtt gcgcagcagc tggcggccga agcgggaagt
    10081 gatgtctttt tgcttaagcg tattcgctat gtcgatgaag aagcggtatc gattgaggaa
    10141 tcgtgggttc cggcgcattt aattcatgat gttgatgcca tcggaatttc tctttatgac
    10201 tatttccgca gccagcacat ttacccacaa cgtacgcgtt cccgcgttag cgcccggatg
    10261 ccggatgccg agtttcagtc acatattcag ttagatagca aaataccggt gctggtgatc
    10321 aagcaagtgg cgcttgacca acagcaacgg cctattgagt acagcattag ccactgtcgc
    10381 agcgatctat acgtttttgt gtgcgaggag tagttcctcg cgcgttggcg cacaatcacc
    10441 gccccgatgc ccgacaaccc acgacgccac tgcattgccc agtaatacgg catccgccag
    10501 tggcagacca gaggccagcc cggcaagtac gccacctgca tgactgtcgc ccgccccaat
    10561 ggtgtctaca acttgcgttg gaaatgccgg aatgcagcca gaagcgtcgt tgctgaaata
    10621 ccatgcgcct tctttatcga gacgaacgat caacggcgcg gcaaatttct cctgccattg
    10681 cttgccaagt gttgttatct ctgcggataa agcaaaacgt tcggcggcaa tctcagcctc
    10741 ttgacgattg agtgacacta aaggtcgaca ggccatgatc cgcgccagta atgcgtccgg
    10801 gatatcgcca atacgtgggc aaaaatcgat aaacggcgtc acgtcttgca gcttttccag
    10861 ccattccact aacaattcgc cgcagggcga ggccagttga taaccggaaa aatagagcag
    10921 gttgccaggc gcaacggtta atcgtgccaa ccattggcga ttccattgat tttccacacc
    10981 gctgaatgac ataaaagtgc gttcgccatc cggctcaacc agtgccagac accagccgtt
    11041 atcgctttcg gcgttatcga tcaggctgat taacccctct ttcgccatcc ggttgcgaat
    11101 aatctccgcc cacacgccct gaccgagcgg caaggcatta cccgcttcga tgccgaggcg
    11161 ctttaacgcc acggcaatat tcagtgcgca gccgccaacg ttaacgctct gctgtttcag
    11221 ttcgatatcg cacccacgcc aggggagggc ataagcgtcg gcgatcacgt caatgaccgc
    11281 cgcgccgacg accatcaccg gctgacgcgt ggttaattca ggcagcagtg tgtgtaatcg
    11341 agcgccgctc ataccgcctc ccgttgttga cgatatttcg ccagcgctgt ggcatagcgg
    11401 ttgaaatcaa gctgatttac cgcatccagc tccgccttta acgcaggatc gacagcgtta
    11461 atgccatgca acgcgccgca aatcgccgtc gccatagcac ctatggtgtc tgtgtcgccg
    11521 ccaagattgg cgcacaggac ggcgcagcgg tttgggtcgg tttgtgccag ttcaactagc
    11581 gcaatggcgc acggaacgga ctcaatagtg ctggtgcctg cgccaacgac ctggtaaagc
    11641 tgttcgctgg cggattcggt gccgtcggca ttacgcacaa ttttcagcgc aatctccaga
    11701 cgtgccgcca gtgaggcgct gaacgtggtg gtgcgttttt gctgtgcatg tcgcgcaatt
    11761 gaaggcaatg aatcaacaat cgctgaccag cgttctccgt caatggcacg agaaatcgcc
    11821 catgcgatga ctaccgcgcc tgcaaccgcc agatcggatt tatgggtcgg gctggacgcc
    11881 agcgccacat catcaataaa ggaatcaaca tcacgcgctg gaagcaaaca acctaatggc
    11941 gagacgcgca tcgctgcgcc gttggttacg ccgttgtttt ccagttcagc aacgggttta
    12001 ccgtcgcgaa tggcgttaag cgcaatcttc gaggtcggac ctagtacgtt tttgttaaag
    12061 gcgtcgaaac gcaacgccca gtcgagaata ttacgcccaa tcagattcgg atcgatcttg
    12121 ccttcacatt ccagtaacgc atccgccaga cacaggccat cgaggtatcg tcggtgaatt
    12181 cggcgcggtt aaaataacag gccgcgttat tctcctttgg tccaggcaga aaacggtcaa
    12241 tccagccaaa gtgtgcttta acgcggctgc gtggccaaag ctcggagggc atccccatcg
    12301 catcccctaa cgcctgccca taaagagcac cgagaatacg ttctgttttc atttaacttc
    12361 cccttgaatc ggaatttacg ggagcatagt aatgacagat attgcgcagt tgcttggcaa
    12421 agacgccgac aaccttttac agcaccgttg tatgactatt ccttctgacc agctttatct
    12481 ccccggacat gactacgtag accgcgtgat gattgacaat aatcgcccgc cagcggtgtt
    12541 acgtaatatg cagacgttgt acaacaccgg gcgtctggct ggcacaggat atctttctat
    12601 tctgccggtt gaccagggcg ttgagcactc tgccggagct tcatttgctg ctaacccgct
    12661 ctactttgac ccgaaaaaca ttgttgaact ggcgatcgaa gcgggctgta actgtgtggc
    12721 atcaacttac ggcgtgttgg tgtcggtatc gcggcgctat gcgcatcgca ttccatttct
    12781 cgtcaaactt aatcacaacg agacgctaag ttacccgaat acctacgatc aaacgctgta
    12841 tgccagcgtg gagcaggcct tcaacatggg cgcggtggcg gttggtgcga ctatctattt
    12901 tggctcggaa gagtcacgtc gccagattga agaaatttct gcggcctttg aacgcgcgca
    12961 cgagctgggc atggtgacag tgctgtgggc ctatttgcga aactccgcct ttaagaaaga
    13021 tggcgttgat taccatgttt ccgccgacct gactggtcag gcgaaccatc tggcggcaac
    13081 cataggtgca gatatcgtca aacaaaaaat ggcggaaaat aacggcggct ataaagcgat
    13141 taattacggt tataccgacg atcgcgtgta cagcaagtta accagcgaaa acccgattga
    13201 tctggtgcgt tatcagttag ctaactgcta tatgggccgg gctgggttga taaactccgg
    13261 cggtgctgca ggcggtgaaa ctgacctcag cgatgcagtg cgtactgcgg ttatcaacaa
    13321 acgcgcaggc ggaatggggc tgattcttgg acgtaaggcg ttcaagaaat cgatggctga
    13381 cggcgtgaaa ctgattaatg ccgtgcagga cgtttatctc gatagcaaaa ttactattgc
    13441 ctgatacgcc tcatacgaac agcaccaccc cgtaggacgg ataagacgtt tacactgcat
    13501 ccggcaacct atgcctgatg cgacgcggaa gcgtcttatc atgcctacat agcactgcca
    13561 cgtatgttta caccgcatcc ggcataaaaa cacgcgcact ttgctacggc ttccctatcg
    13621 ggaggccgtt tttttgcctt tcactcctcg aacaattttc atattgttgt ttttgcgatc
    13681 attatctcga catttaaaag caaataattt cattatattt tgaaatcgaa agcaaacgac
    13741 aagatatgaa aatgtacgtg gtatcgacaa agcagatgct gaacaacgca cagcgcggcg
    13801 gttatgcggt tccggcattc aatattcaca atctcgaaac gatgcaagtg gtggtggaaa
    13861 ccgctgccaa cctgcatgcg ccggtcatca tcgccggaac gcctggcaca tttactcatg
    13921 ctggtacaga aaatctgttg gcgctggtca gcgcgatggc gaagcaatat caccatccac
    13981 tggcaattca tctcgaccat cacacgaaat ttgacgatat cgctcagaag gttcgttctg
    14041 gcgtgcgctc agtcatgatt gacgcctcgc atttgccttt tgcgcaaaat atttcacggg
    14101 tcaaagaggt ggtggatttt tgccatcgct ttgatgtcag cgtcgaggcg gagctggggc
    14161 aacttggcgg ccaggaagat gatgtgcaag tcaatgaagt cgatgcgttg tacaccaacc
    14221 ccgcacaggc gcgtgaattt gccgaggcaa ccgggattga ttccctggcg gtcgccatcg
    14281 gcacggctca cggtatgtat gccagcgcac cggtgcttga tttttctcga ctggagaaca
    14341 ttcgccagtg ggtgaactta ccgctggtgc tgcatggcgc gtcaggatta tcgactaagg
    14401 atattcagca aaccatcaaa ctggggatat gcaaaatcaa cgttgcaacg gagctgaaaa
    14461 atgccttctt gcagtcgtta aaaaattacc tgaccgagca ccccgaagcg accgatcccc
    14521 gggattattt gcagtcggct aaatccgcaa tgcgcgatgt ggtgagcaaa gtgattgccg
    14581 attgtggctg cgagggcagg gcataacgca cctgccattt aacaaggaaa aaacatgaaa
    14641 acgttaattg cccggcataa agctggtgaa catatcggca tatgttcagt ctgttctgcc
    14701 catccgttgg ttatcgaagc ggcgctggca tttgatcgca acagcacgcg caaagtgctg
    14761 attgaagcaa cgtcaaacca ggtcaatcaa tttggcggtt ataccggaat gacaccggca
    14821 gactttcgcg aatttgtttt tacgattgcc gataaagttg ggtttgcacg cgaacgcatt
    14881 attctcggcg gcgatcatct gggaccaaac tgctggcagc aagaaaatgc ggatgcggcg
    14941 atggaaaaat ccgtcgagct ggtaaaagca tatgttcgtg ctggcttcag taaaattcat
    15001 cttgatgcgt caatgtcctg cgcgggggat accataccgt tagcaccaga aacggttgcg
    15061 gaacgagctg ctgtgctttg ctttgctgcg gaaagtgtgg cgacagattg ccagcgtgag
    15121 caactgagct atgtcattgg cactgaagtt ccggttccgg gcggtgaggc cagcgccatt
    15181 cagtcagtac acatcaccca tgttgaagat gccgccaata ctttacgtac gcatcaaaag
    15241 gcctttattg cccgtgggct gacagaggcg ttaacacgtg tgattgccat cgtggtgcag
    15301 ccgggtgtgg aatttgatca cagcaatatt atccattatc agccgcagga agcgcaggca
    15361 ctggcgcaat ggatagaaaa cacccgaatg gtttatgaag cacattctac cgattaccag
    15421 acccggacgg cttattggga attagtccgc gatcactttg caatattgaa agtcggtccc
    15481 gcattaacct ttgctttacg cgaggcgata tttgcactgg cacaaattga gcaggaactt
    15541 atcgcccccg aaaatcgcag cggttgcctg gcggtaattg aagaagtgat gctcgacgaa
    15601 ccgcaatact ggaaaaaata ttatcgcacg ggttttaacg attcattact ggatattcgt
    15661 tacagcctgt cggatcgtat tcgttattac tggccgcata gtcggattaa aaatagcgtt
    15721 gaaacgatga tggtgaatct gcaaggcgtg gacatcccac tgggcatgat tagtcagtat
    15781 cttcccaaac aatttgaacg cattcagtcc ggggaattat cagcaatgcc gcatcagctt
    15841 attatgaata aaatttatga tgttttgcgc gcctatcgct acggctgtgc ggaataagga
    15901 cggtatatga ctaacctgtt tgttcgtagc ggaatttctt ttgtcgatcg tagcgaagtt
    15961 ttaacctata tcggtaatga gatgctcgcg aaaggtgtgg ttcatgatac ctggccacag
    16021 gcattaattg ccagagaagc agaattccct accgggataa tgcttgagca gcacgccatt
    16081 gcaataccgc attgtgaggc gattcatgct aagtcgtcag ccatttatct gttaaggcca
    16141 acaaataaag ttcattttca gcaagcggat gatgataacg acgtggcggt atcgttggtt
    16201 attgcgttga ttgtggaaaa tccgcagcag caattgaaac ttttacgctg tttatttggc
    16261 aagttacaac agcccgatat cgtcgagaca ctaattactc ttcctgaaac tcagttaaag
    16321 gaatacttca caaagtatgt tttagattca gacgaataaa tccctctgta acaataaaaa
    16381 ggactattta tgaaacgcaa gattattgtc gcttgcggag gcgcggttgc gacctctacg
    16441 atggcggcgg aagaaattaa agagttgtgt cagagtcata atattcctgt tgaattaatc
    16501 cagtgtcggg ttaatgaaat agaaacctat atggatggtg tgcatttgat atgtaccact
    16561 gccagggtgg atcgtagttt tggcgatatt ccgttagttc acggcatgcc ttttgtttct
    16621 ggtgtcggta tcgaagcatt acaaaataaa attctgacta tcttacaggg atgacctatg
    16681 ttttcagaag tcatgcgtta tattctcgac ctcggcccta cagtgatgct gccggttgtc
    16741 atcattattt tttctaaaat attaggcatg aaggcaggcg attgctttaa agcgggtctg
    16801 catatcggga ttggctttgt tggcattggc cttgtgattg gcttaatgct ggattccatt
    16861 ggtccggcgg cgaaagcgat ggcggaaaat ttcgacctga atctgcatgt ggtcgatgtc
    16921 ggctggccgg gctcttcacc aatgacctgg gcgtcgcaaa ttgcgctggt ggcgattccg
    16981 attgcgatat tggttaacgt ggcgatgtta ctgacccgta tgacgcgggt ggtaaatgtt
    17041 gatatctgga atatctggca tatgaccttc accggcgcgt tgctgcacct ggcaaccggt
    17101 tcatggatga tagggatggc gggtgtggta attcacgcgg cgtttgttta taagctcggc
    17161 gactggtttg cccgcgatac ccgaaatttc tttgagctgg aaggtattgc tattccgcac
    17221 ggtacgtcgg cgtatatggg gccgattgcg gtgctggtcg atgctatcat cgagaaaatc
    17281 ccaggcgtta accgaattaa atttagcgcc gacgatattc agcgcaaatt tggccctttt
    17341 ggcgaacctg tcaccgtggg ttttgtgatg gggctgatta tcggcatcct cgcgggttac
    17401 gatgtcaaag gtgtattgca gctggcggta aaaacggcgg cagttatgct gctgatgcca
    17461 cggatgatta aacccatcat ggatggttta acgcccatcg ctaagcaggc ccgtagtcgt
    17521 ttacaggcga agttcggcgg tcaggagttc ctgattggcc tggatcccgc attactgctg
    17581 gggcatacgg cggtggtatc ggcaagcctg atttttattc cgctcaccat tttaattgct
    17641 gtttgtgttc cgggtaatca ggtactgccg tttggcgatc ttgccactat cggcttcttc
    17701 gtggcgatgg cggtcgcggt gcatcgtgga aatctgttcc gcaccttaat ctcgggtgtc
    17761 atcattatga gcatcaccct gtggatcgcg acgcaaacta ttggtttgca cacccaaccg
    17821 gcggctaatg ctggggcgtt aaaagccggg ggtatggtgg cttcaatgga tcagggcggt
    17881 tctcccatta cctggttact gattcaggtt ttctccccgc aaaatattcc cggtttcatt
    17941 attatcggcg caatttatct gaccggtatt ttcatgacct ggcgtagagc gcgtggtttt
    18001 actaaacaag agaaagccgt tctcgcagaa taatttttac ctgaggggga ggttatcccc
    18061 cttaacaa
//
LOCUS       DAJTSF010000080        17926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000080 DAJTSF010000000
VERSION     DAJTSF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.080"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            322..1005
                     /locus_tag="OO335_002891"
     CDS             322..1005
                     /locus_tag="OO335_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6003205.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1049..5711
                     /locus_tag="OO335_002892"
                     /pseudo
     CDS             1049..5711
                     /locus_tag="OO335_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6079..7734
                     /gene="lldP"
                     /locus_tag="OO335_002893"
     CDS             6079..7734
                     /gene="lldP"
                     /locus_tag="OO335_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6003206.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7734..8510
                     /gene="lldR"
                     /locus_tag="OO335_002894"
     CDS             7734..8510
                     /gene="lldR"
                     /locus_tag="OO335_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6003207.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8507..9697
                     /gene="lldD"
                     /locus_tag="OO335_002895"
     CDS             8507..9697
                     /gene="lldD"
                     /locus_tag="OO335_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6003208.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9893..10366
                     /gene="trmL"
                     /locus_tag="OO335_002896"
     CDS             9893..10366
                     /gene="trmL"
                     /locus_tag="OO335_002896"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6003209.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10419..11240)
                     /gene="cysE"
                     /locus_tag="OO335_002897"
     CDS             complement(10419..11240)
                     /gene="cysE"
                     /locus_tag="OO335_002897"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6003210.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11320..12339)
                     /gene="gpsA"
                     /locus_tag="OO335_002898"
     CDS             complement(11320..12339)
                     /gene="gpsA"
                     /locus_tag="OO335_002898"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6003211.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12339..12806)
                     /gene="secB"
                     /locus_tag="OO335_002899"
     CDS             complement(12339..12806)
                     /gene="secB"
                     /locus_tag="OO335_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6003212.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12869..13120)
                     /gene="grxC"
                     /locus_tag="OO335_002900"
     CDS             complement(12869..13120)
                     /gene="grxC"
                     /locus_tag="OO335_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6003213.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13262..13693)
                     /locus_tag="OO335_002901"
     CDS             complement(13262..13693)
                     /locus_tag="OO335_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6003214.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13938..15482
                     /gene="gpmM"
                     /locus_tag="OO335_002902"
                     /gene_synonym="pgmI"
     CDS             13938..15482
                     /gene="gpmM"
                     /locus_tag="OO335_002902"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6003215.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15492..16775
                     /gene="envC"
                     /locus_tag="OO335_002903"
     CDS             15492..16775
                     /gene="envC"
                     /locus_tag="OO335_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6003216.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16779..17738
                     /locus_tag="OO335_002904"
     CDS             16779..17738
                     /locus_tag="OO335_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6003217.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17725..17926)
                     /locus_tag="OO335_002905"
                     /pseudo
     CDS             complement(17725..17926)
                     /locus_tag="OO335_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4501 a         4642 c         4661 g         4122 t
ORIGIN      
        1 cgtctctttt tttcggattt aattgtgaaa ttttaatcat catattcaca aaatcaatta
       61 tctgtgaaat ttttcccaga catggtttct ttcatatttg acatttatta ataatcatca
      121 ttagaatcca cgaaaaataa tatattcata caaacacaga catcttttga aacgaatgat
      181 acatttttat gatgaatgct taaaacaata tttaatcgac acacatcact gtcattaatg
      241 taaagcaaca tagctcacca ataatacaat ggacaactct accggcattg agcgctggtc
      301 ggaggaaata aggattttta catgaacttg aagaagattt ttttcagtgc tgtcactgtt
      361 tcagttctct gcgcattaac gggatgtgac tacattgaag aaggcaagcc agaaagtagt
      421 ttactgaaac aacaagaaga acataataat aaaattgtgc tacttgaaaa acaacaagca
      481 cagctaaaga gtcaactcga aactattcaa aaacaacaaa ctggcataat aaacagtaca
      541 aagactttaa ctcacgtaat taaatccgtt aaagaccaac aaaatacttt tatttttacc
      601 gagtttaacc cagcaaaaac gaagtatttc atcctgaaca acggttcggt ggctttagcg
      661 ggtcgagtgt tatccattga cgccacagag aatggtagtg ttattcatat ctcactggtc
      721 aacttattaa gtacacctat ctcaaacatt ggttttaatg cgacatgggg tggtgaaaaa
      781 cctgtcgatg ccaaagagtt tgcccggtgg caacaattac ttttcaacac atcaatgaaa
      841 tccacattga aattattacc aggtcaatgg caagacatta atttgaccct gaagggtgta
      901 tcgcccaata acctgggata tctgaagtta gccatcaaca tggaaaatat tcagttcgac
      961 aatcttccct ctgctgaaaa tcggcagaaa agaagcaaaa aataaatgca gttagtacgc
     1021 ttataacaag gaatagcgag catcattaat gaacaaaata tttaaagtta tctggaaccc
     1081 tgcgacaggg aattatactg ttaccagcga aacggcaaaa agccgtggca agaaatctgg
     1141 gcgcagtaag ctgttaattt ctgcgctggt tgcgggtgga atgttgtcgt cgtttggggc
     1201 attggcgaat gccgggaatg acaacggtca gggtgttgat tacggtagtg gatcagctgg
     1261 cgacggctgg gttgctatag gcaaaggggc gaaagcaaat acttttatga acaccagtgg
     1321 ttccagtact gctgtgggtt atgacgctat agctgaaggc caatatagct ctgccatcgg
     1381 gtcaaaaacc catgcgattg gtggtgcatc aatggccttt ggggttagtg caatatcaga
     1441 aggcgataga agtatagcac tgggtgcctc ttcgtattca ttgggccaat actcaatggc
     1501 cctcggccgt tattcaaaag cattgggtaa attgtctatt gctatggggg actcttccaa
     1561 agcggaagga gcaaacgcca ttgccctggg gcgtagcagt gtagctagcg gtactgacag
     1621 cctcgcattt ggcagacaat cacttgccag cgcagcgaac gctattgcga taggtgctga
     1681 gaccgaagcc gctgaaaatg caactgctat tggcaataat gcgaaggcaa aagggactaa
     1741 tagcatggca atggggttcg gaagccttgc cgataaagtc aatactatcg cattaggaaa
     1801 tggcagccag gctctggcag ataatgcaat cgccataggc cagggcaaca aagctgatgg
     1861 cgtggatgcc atcgctctgg gtaatggtag ccagtcgaga ggcttaaaca ccattgcctt
     1921 aggcacagcc agtaatgcaa ctggtgataa gagtcttgcg cttggtagta atagcagtgc
     1981 caacggtatt aactctgtcg cgctgggcgc agattccatt gcggatttag acaataccgt
     2041 ctctgtcggc aatagttcat taaaacgcaa gatcgttaat gtgaaaaatg gcgcgatcaa
     2101 gtctgacagt tacgatgcca ttaatggttc acagctttat gccattagcg actcggtagc
     2161 aaaaaaggct tggaggaggg gctgcagtag atgttgatga cggtactgtt acagcaccaa
     2221 cctacaattt aaaaaatggt agcaaaaata acgtaggggc tgcgctcgct gtacttgatg
     2281 aaaacaccct gcaatgggac caaaccaaag gcaaatacag cgctgctcat ggtactagta
     2341 gcccaactgc cagcgtaatc accgatgttg cggatggcac gatttcagcc tccagtaagg
     2401 atgcggttaa cggttcccaa ctgaaagcta ccaatgacga tgtcgaagcc aacaccacca
     2461 atatcaccaa cctgacggat tccgttggtg accttcaggc tgatgccctg ctctggaacg
     2521 aaactaaaaa ggcattcagt gcagctcacg gccaggatac caccagcaaa atcaccaacg
     2581 ttaaagatgc cgacctgacg gctgacagca ctgatgctgt taacggctct cagctgaaaa
     2641 ccaccaacga tgctgtggcg acgaatacca ccaatatcgc caataacact tccaatattg
     2701 ccactaacac caccaacatc tctaacctga ctgagacggt gactaatctt ggtgaggatg
     2761 cgctgaaatg ggataaggac aatggtgtat tcacggcagc tcatggcacc gagaccacca
     2821 gcaaaataac caacgttaaa gatggcgacc tgacgactgg cagcaccgat gccgttaacg
     2881 gctctcagct gaaaaccacc aacgatgccg tggcgacgaa taccaccaat atcgccacta
     2941 acaccaccaa catctctaat ctgactgaga cggtgactaa tcttggtgag gatgcgctga
     3001 aatgggataa ggacaatggt gtcttcactg cagctcatgg caacaatacc gccagcaaaa
     3061 tcaccaatat cctggacggc acagtcactg caaccagttc cgatgccatt aacggtagcc
     3121 agctttatga cttaagcagc aatatcgcca cctacttcgg cggcaatgct tctgtgaata
     3181 ctgacggtgt gtttaccggt ccaacctaca aaatcggtga aacaaattat tataacgtcg
     3241 gcgatgcact ggctgcgatt aactcctcat ttagcacgtc tctcggcgat gctctgcttt
     3301 gggatgccac cgcaggtaaa ttcagtgcca aacacggtac taatggtgac gcaagcgtga
     3361 tcactgatgt cgcagatggt gaaatttcag actccagttc tgacgcagta aacggctcac
     3421 aactccacgg cgtgagcagt tatgttgttg atgcgctggg gggtggtgcc gaagtcaatg
     3481 cagacggcac catcactgcg ccgacgtaca ccattgctaa tgctgattac gataatgtcg
     3541 gtgatgccct gaatgctatc gataccactc ctgacgacgc tctgctctgg gatgcggacg
     3601 ccggtgaaaa tggtgcattt agcgccgctc acggaaaaga taaaactgcc agtgtaatca
     3661 ctaacgtcgc taacggtgca atctctgctg ccagcagcga cgcgattaac ggctcacaac
     3721 tctataccac caataagtac atcgctgatg cgctgggtgg tgacgcagaa gtcaacgctg
     3781 acggcaccat caccgcaccg acttacacca ttgcgaacgc cgagtacaac aacgtcggtg
     3841 acgccctgga tgcgcttgat gataacgccc tgctgtggga taagactgcc aatggcggtg
     3901 ctggagccta caatgccagc catgacggta aagccagcat catcactaat gtcgctaatg
     3961 gcagtattag tgaggacagt accgatgcag tgaacggttc tcagttgaat gcgacgaata
     4021 tgatgattga gcagaacacc caaattatca atcagctcgc tggtaacacc gacgcaacct
     4081 atatcgaaga aaatggcgct ggtatcaact acgttcgtac taacgacaac gatttagcct
     4141 ttaacgatgc aagcgcctct ggtgtcggcg ctacagctgt aggttataac gctgtcgcgt
     4201 ctggtgccag cagcgtagcc attggtcaga acagcagcag caccgttgat accggtattg
     4261 cgctgggtag cagctccgtt tccagccgtg tgattgccaa aggttctcgt gacactagcg
     4321 taacggaaaa tggcgtggtg attggttatg acaccactga cggcgaactg ctaggtgcat
     4381 tgtcaattgg tgatgacggt aaataccgcc aaatcatcaa cgtagctgat ggttcagaag
     4441 cccatgacgc cgttacggtt cgccagttgc agaacgctat tggagcggtc gccactacgc
     4501 caaccaagta ctttcacgcc aactcaacgg cagaagactc actggccgtt ggtgaagact
     4561 cgctggcaat gggtgcgaaa actgtcgtta atggtaatgc aggtattggt atcggtttga
     4621 acactctggt tctggctgat gcgatcaacg gcattgctat cggcagtaac gcacgcgcaa
     4681 accatgcaaa cagtatcgca atgggtaacg gttctcagac cacccgtggt gcacagactg
     4741 gctacaccgc ctacaacatg gacgcaccgc agaactctgt aggtgagttt tctgtcggta
     4801 gtgaagacgg tcaacgtcag atcacaaacg tcgcagctgg ttcagcggat accgatgcgg
     4861 ttaacgtggg tcagttgaaa gtcactgatg agcgcgtagc gcaaaatacc cagagcatta
     4921 ctaacctgaa caatcaggtc actaatctgg atactcgcgt tactaatatc gaaaacggta
     4981 ttggcgacat tgtcaccacc ggtagcacca agtacttcaa gaccaacacc gatggcgtag
     5041 atgccaacgc ccagggtaaa gatagcgttg ctattggttc tggttccatt gctgccgctg
     5101 acaacagcgt cgcactgggt accggttccg ttgcagagga agaaaataca atctctgtag
     5161 gttcttctac taaccaacgc cgtatcacca acgtagctgc cagtgttaat gccaccgatg
     5221 cggttaacgt gtcgcagctg aaatcttctg aagcaggcgg agtgcgctac gacaccaaag
     5281 ctgatggttc tatcgactat agcaatatca ccctcggtgg tggcaacggc agtacgactc
     5341 gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc ggtgaactac gcgcagttga
     5401 agcaaagcgc gcaggaaacg aagcaataca ccgatcagcg gatggttgag atggataaca
     5461 aactgtctaa aactgaaagc aagttgagcg gtggtatcgc ttctgcaatg gcaatgaccg
     5521 gtctgccgca ggcttataca ccgggtgcca gcatggcttc tattggtggc ggtacttaca
     5581 acggtgaatc ggcagttgct ttaggtgtat cgatggtgag cgccaatggt cgttgggtct
     5641 acaaattaca aggtagtacc aatagccagg gtgaatactc cgccgcactc ggtgccggta
     5701 ttcagtggta atgatccatt aagttagtgt gactaagggc aaacgccaaa ttgcctgatg
     5761 cgttacgcgc atcaggcaag ataaggcgct ccgggtcaac aatctaaggg caattctctg
     5821 atgaggattg cccttttctt taccagacat ctccccccac aagaattggc cctaccaatt
     5881 cttcgcttat ctgacctctg gttcacaatt ccccaattaa aactcacatc aatattgcca
     5941 atacataaca tttagttaac cattcattgt cattatccct acacaacaca attggcagtg
     6001 ccacttttac acaacgtgtg acaaggagat gagcaacaga ctcattacac gatgtgcgtg
     6061 gactctagga gacctgcaat gaatctctgg caacaaaact acgatcccgc cgggaatatc
     6121 tggctttcca gtctgatagc atcgcttccc atcctgtttt tcttctttgc gctgattaag
     6181 ctcaaactga aaggatacgt cgccgcctca tggacggtag caatcgccct tgccgtggct
     6241 ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct cggtggttta tggcttcttc
     6301 tatggtttgt ggcccatcgc gtggatcatt attgcagcag tgttcgtcta taagatctcg
     6361 gtgaaaaccg ggcagtttga catcattcgc tcgtctattc tttcgataac ccctgaccag
     6421 cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt tccttgaagg agccgcaggc
     6481 tttggcgcac cggtagcaat taccgccgca ttgctggtcg gcctgggttt taaaccgctg
     6541 tacgccgccg gactgtgcct gattgttaac accgcgccag tggcatttgg cgcgatgggc
     6601 attccaatcc tggttgccgg acaagtaaca ggcatcgaca gctttgagat tggccagatg
     6661 gtggggcgtc agctaccgtt tatgaccatt atcgtgctgt tctggatcat ggcgattatg
     6721 gacggctggc gcggtatcaa agagacgtgg cctgcggtcg tggttgcagg cggctcgttt
     6781 gccatcgctc agtaccttag ctctaacttc attgggccgg agctgccgga cattatctct
     6841 tcgctggtat cactgctctg cctgacgctg ttcctcaaac gctggcaacc agttcgcgta
     6901 ttccgcttcg gtgacttggg ggcgtcacag gttgatatga cgctggccca caccggttac
     6961 accgcgggtc aggtgttacg tgcctggaca ccgttcctgt tcctgaccgc taccgtaaca
     7021 ctgtggagca tcccgccgtt taaagccctg ttcgcatcgg gtggtgcgct gtatgagtgg
     7081 gtgatcaaca ttccggtgcc gtacctcaat aaactggttg cccgtatgcc accagtggtc
     7141 agcgaggcta cagcctatgc cgccgtgttt aagtttgact ggttctctgc caccggcacc
     7201 gccattctgt ttgctgcact gctctcgatt gtctggctga agatgaaacc gtctgacgct
     7261 atcagcacct tcggcagcac gctgaaagaa ctggctctac ccatctactc catcggcatg
     7321 gtgctggcgt tcgcctttat ctcgaactat tccggactgt catcaacact ggcactggca
     7381 ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt tcctcggctg gctgggggta
     7441 ttcctgaccg ggtcggatac ctcatctaac gccctgttcg ccgcgctgca agccaccgca
     7501 gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg ccaataccac cggtggcgtc
     7561 accggtaaga tgatctcccc gcaatctatc gctatcgcct gtgcggcggt aggcctggtg
     7621 ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca gcctgatctt cacctgtatg
     7681 gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt ggatgattcc ttaatgattg
     7741 ttttacccag acgcctgtca gacgaggttg ccgatcgtgt gcgggcgctg attgatgaaa
     7801 aaaacctgga agcgggcatg aagttgcccg ctgagcgcca actggcgatg caactcggcg
     7861 tatcacgtaa ttcactacgc gaggcgctgg caaaactggt gagcgaaggc gtgctgctca
     7921 gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga cacatggtcg gagcaaaaca
     7981 tcgtccagcc gctaaaaacg ctgatggccg atgatccgga ttacagtttc gatattctgg
     8041 aagcccgcta cgccattgaa gccagcaccg catggcatgc ggcaatgcgc gccacacctg
     8101 gcgacaaaga aaagattcag ctttgctttg aagcaacgct aagtgaagac ccggatatcg
     8161 cctcacaagc ggacgttcgt tttcatctgg cgattgccga agcctcacat aacatcgtgc
     8221 tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc ctcagtgaag catagccgtc
     8281 agcggatgta tctggtgcca ccggtttttt cacaactgac cgaacaacat caggctgtca
     8341 ttgacgccat ttttgctggt gatgccgacg gggcgcgtaa agcaatgatg gcgcacctta
     8401 gttttgttca caccaccatg aaacgattcg atgaagatca ggctcgccac gcacggatta
     8461 cccgcctgcc cggtgagcat aatgagcatt cgagggagaa aaacgcatga ttatttccgc
     8521 agccagcgat tatcgcgccg cagcgcaacg cattctgccg ccgttcctgt tccactatat
     8581 ggatggtggt gcatattctg aatacacgct gcgccgcaac gtggaagatt tgtcagaagt
     8641 ggcactgcgc cagcgtattc tgaaaaacat gtccgactta agcctggaaa cgacgctgtt
     8701 taatgagaaa ttgtcgatgc cggtagcact ggcaccggtg ggtttgtgtg gcatgtatgc
     8761 gcgtcgtggc gaagttcagg cagccaaagc ggcggacgcg catggcattc cgtttactct
     8821 ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca gccatcaagc gcccaatgtg
     8881 gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt aacgcgctgg agcgagcaaa
     8941 agcagcgggt tgttcgacgc tggttttcac cgtggatatg ccgacaccgg gcgcacgcta
     9001 ccgtgatgcg cattcaggta tgagcggccc aaatgcggca atgcgccgct acttgcaggc
     9061 ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac ggtcgtccac atgatttagg
     9121 taatatctca gcttatctcg gcaaaccgac cggactggaa gattacatcg gctggctggg
     9181 gaataacttc gatccgtcca tctcatggaa agaccttgag tggatccgcg atttctggga
     9241 tggcccgatg gtgatcaaag ggatcctcga tccggaagat gcgcgcgatg cagtacgttt
     9301 tggtgctgat ggaattgtgg tttctaacca cggtggccgc cagctggacg gtgtactctc
     9361 ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa ggtgatatcg ccattctggc
     9421 ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg attgcgctcg gtgccgacac
     9481 cgtactgctg ggtcgtgctt tcctgtatgc gctggcaaca gcgggccagg cgggtgtagc
     9541 taacctgcta aatctgatcg aaaaagagat gaaagtggcg atgacgctga ctggcgcgaa
     9601 atcgatcagt gaaattacgc aagattcgct ggtgcagggg ctgggtaaag agttgcctac
     9661 ggcactggct ccgatggcga aagggaatgc ggcatagtcg tttgcgcccc ctcaccctaa
     9721 ccctctccct cagggagagg ggaccgttcg gcgctgtatg tactccctca ctctgaaacg
     9781 acaccgcact ctttttttct ccctcgcccc tccggggagg gccggggtga ggggaaaagg
     9841 ccgcacggtt caccaatttt ctgctatcct gcccccgcac taagggggca gtatgctaaa
     9901 catcgtactt tacgaaccag aaattccgcc aaatactggc aacatcatcc gtctttgcgc
     9961 taataccggc tttcgtctac atatcatcga accgatggga tttgcctggg acgacaagcg
    10021 cctgcgccgc gcggggctgg actatcacga gtttaccgcc gttacgcgtc atcatgacta
    10081 tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt gccctcacca cgaaaggtac
    10141 gcctgctcac agcgccgtaa gctatcagga tggcgattat ctgatgttcg gcccggaaac
    10201 acgcggcctg ccagcgagca ttcttgatgc cctgcccgct gaacaaaaaa ttcgcattcc
    10261 gatggtgccg gacagccgca gcatgaatct gtccaatgcg gtgtcggtag tggtgtatga
    10321 agcctggcgg cagctgggat atccgggagc gttgttgaga gattagttac tgtatgccgg
    10381 atagatgatt acatcgcatc cggcacgatc acaggacatt agatcccatc cccatactca
    10441 aatgtatggt taataccgtt gaaatgctgg tccatatcca ttgatggctt atcgctgtct
    10501 ggtttaccga caatacgagc cggaacgcca gcggcggttg tatgcggcgg caccggttgc
    10561 agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa cttcaatatt gccgaggatt
    10621 ttcgcgcccg cgccaatcat cacaccttca cgaattttcg ggtgacggtc accaccagat
    10681 ttacccgtac cgccaagcgt cacagattgc agaatcgata cgtcgttttc aattaccgcc
    10741 gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa tgccacggcc aatttttgct
    10801 gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt tttgcagaaa gattgccaat
    10861 gcgcgacgcc cctgattcca caaccagtga ccgatgcgat aggcctgcaa ggcatgaaaa
    10921 cccttcaggt ataacaacgg ggttgagtat ttatcgactg ccgggtcgcg ggtacgcacc
    10981 gcctgaatat cacaggccgc agaggcgatc atttccgggt cagcggcgta ggcttcttca
    11041 accacttcac ggatagcaat agcaggcata attggcgatg acagcttgtt cgccagcatg
    11101 tagctcagtg cactgccaag gttttcgtgc ttgagtagcg tcgcgtggta aaaactggcc
    11161 agcattggct cacagtccgc cagcgttctg gcttcggctt taatattgtt ccagacaatt
    11221 tccagttctt cacacgacat tgcttactcc acacgatgag ataatgaccg gcccgttctg
    11281 cgcgggccgg gtcatagcgg taacaaaggt tccctggggt tagtgactgc tgcgctcgtc
    11341 cttgcgtgca cgacctaata atgtcaatgc tgcctcgcgc gcgttttttc cgcaatataa
    11401 tacttgataa atttcctcgg ttattggcat ttcaacgccg aagcgatgcg ccagttcgcg
    11461 gacttctttc gtattgcggt agccttccac cacctgacca atcttctcct gcgcgctttg
    11521 tacatccatg ccctgaccga gcatcatacc aaaacggcgg ttacgcgact ggttgtcggt
    11581 acaggtaagc accagatcgc ccagccccgc catgcccata aaggtggcag gatcggcacc
    11641 cagcgccgca ccaagacgcg acatttcagc cagcccacgg gtgatcagcg ccgtacgcgc
    11701 attcgcacca aaaccgatac cgtcagacat ccccgcgcca atggcaatga cgtttttcac
    11761 cgcgccgcca agctgcacgc caatgaaatc cgggttgctg taaacgcgga aacttttgcc
    11821 gcagtgcagc agctgctgga gatcatcggc aaaggtctga tcggtcgagg ccagcgaaat
    11881 agctgtcggt aaacctgccg ccagttcttt cgcaaacgtt gggccagaga taaccgccag
    11941 cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt aacagacgtc cggtttccgc
    12001 ttccagccct ttggtcgccc acaccagacg cgcatcagga cgcatcagcg gtttaatctg
    12061 gcgcagcact tcaccaaaga catggctggg tacgacgacg agaatattac gactggctgc
    12121 cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg ggaaaaggca catcggggag
    12181 aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata tgttcagggt catggcccca
    12241 gaggacaacc tcgtggccat ttcttgccag ggtaatggca agagcggtgc cgtacgagcc
    12301 ggcaccgatc acagtcattg aagcattacg ttggttcatc aggcatcctg atgttcttca
    12361 gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca gcgcatcgaa gttaaccggc
    12421 gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc tggtgatgca ctcacgagca
    12481 tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat gtgccatctg ggtgccttcg
    12541 atacccgcga tggagaaaat accgccctgc tgaacttcac acaggaacgc ggtttcttcg
    12601 cccaaggagg ccgttacggt aacacgcagt accacttcgt atacgtcatc tgccagttgg
    12661 gaagatgccg tatccagatc aagtttaact tctggttgcc aatctttctg gaaaacgtgc
    12721 ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa tacgttggat ctggaaagtc
    12781 atttcagtgt tgttttgttc tgacatgtgt agaaaaccct taagtgttgt ccttaaatac
    12841 tgcgcaatgt aataccaacg ttcacacgtt atttcagcag gggatccagt ccaccacgtg
    12901 catccaatgc atacaagtca tcacagccgc caatgtgctg tgcgtcaata aaaatctggg
    12961 gaaccgtggt gcaaccgctg cgtttgatca tctcttcacg cttggcggca ttgccatcga
    13021 tcggcaactc ctggaaactc acgcccttgc tgctcagcag tgcttttgca cgatggcaat
    13081 acgggcaggt ttctttggta tagatttcaa cattggccat aatttagctc ctgttttttt
    13141 accctgaaat ttcatgttgc agggcggctg caaaatggag tgtccccaga agcttataac
    13201 aataagtcgc tgggggccta attgcagcta acggcctgca tcatgaaaga cgacaggtaa
    13261 attatttgcc gcgcaccaga ggcaagtttt cgccagccca gccagcaacg ccctctttca
    13321 gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt tgcaggctcc tggcactgca
    13381 tgccagaacc gtctaccacg ataaccgatt tgtctttgtg cttctcaagc tcaccaacat
    13441 tgttggcttt gatttcgctc ggcaacagat tgatagaacc tgcgatatgg cctttacgga
    13501 agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc tttgttgatc agacgcgtag
    13561 cttcaccacg agtaatcacc ttcactttcg aggtcaggct cttaaacgta gtcacaagaa
    13621 ccgccaccag taacgcgatc caggcgatac tcagtatggg atgacggcca acaaattgca
    13681 taatttcttg catggggggt aacaactccc gacgtagtga ttaaaaaaac caggaaagga
    13741 gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa ctggaatgca attttgcggg
    13801 gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc gcgagcggtc gtatattttg
    13861 atcttcagag gctattttat cgattcagct gtagtaaaat tacgcaaatt ttgactcttg
    13921 agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat ggtactggtg attctggatg
    13981 gctatggcta tcgtgaagaa cagcaggata acgccatttt tagtgctaaa accccggtaa
    14041 tggatgcact gtgggccaat cgtccgcata ccctgatcga cgcttccggt ctggaagtcg
    14101 gtctgcctga ccgtcagatg ggtaactccg aagtaggcca cgttaacctg ggtgccggcc
    14161 gcatcgtgta tcaggacctg actcgtctgg acgttgaaat caaagatcgc gctttctttg
    14221 ctaatccagt gctggctggc gcggtacata aagcgaaaaa cgcaggtaaa gcagtacaca
    14281 ttatgggtct gctctccgca ggcggcgtac acagccacga agatcacatc atggcgatgg
    14341 tagaactggc agctgaacgc ggcgcagaaa aaatctacct gcacgcattc cttgacggtc
    14401 gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa atttgaagag aaatttgctg
    14461 cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta ctacgcgatg gaccgcgata
    14521 accgttggga tcgcgttgaa aaagcctatg acctgctgac tctggcgcag ggtgagttcc
    14581 aggccgatac tgccgttgct ggtttgcagg cagcttatgc tcgcgacgaa aacgacgagt
    14641 tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga tgcggcaatg gaagacggcg
    14701 atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg tgaaatcact cgtgcattcg
    14761 tgaacgctga tttcgatggc ttcgcgcgta agaaagtggt taacgtcgat ttcgtgatgc
    14821 tgaccgaata cgccgctgac atcaaaactg cggttgctta cccacccgct tccctggtta
    14881 acaccttcgg cgagtggatg gcgaaaaacg acaaaactca gttgcgtatt tccgaaaccg
    14941 aaaaatatgc ccacgttact ttcttcttca acggtggcgt agaagagtcg ttcaaaggcg
    15001 aagatcgcat tctgatcaac tcaccgaaag tggctaccta cgatctgcaa ccggaaatga
    15061 gctccgcaga gctgaccgaa aaactggttg cggccatcaa gagcggcaaa tacgacacca
    15121 ttatctgtaa ctatccgaac ggcgacatgg taggtcacac cggggtgatg gaagcggcgg
    15181 ttaaagcggt tgaagcactg gatcactgcg tggaagaagt cgcgaaagcg gttgagtccg
    15241 tgggtggaca actgctgatc accgctgacc acggtaacgc tgaacagatg cgcgatccgg
    15301 caacgggtca ggcacacacg gcacacacca acctgccagt tccgctgatt tacgttggtg
    15361 ataagaacgt gaaagcggtt gcaggcggca aactttctga catcgcgccg accatgttgt
    15421 cgctgatggg tatggaaatc ccgcaagaga tgactggtaa gccgctgttc atcgtggaat
    15481 aatccctccc catgagggga aaggcgatta ataccatgac acgggccgtg aaaccgcgca
    15541 ggtttgcaat caggcccatc atctacgcca gcgttcttag cgctggcgta ttgttgtgcg
    15601 ccttttccgc ccacgcggat gagcgtgacc aactcaaatc tattcaggcc gatatcgccg
    15661 caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc aagcctgctc gcacaattga
    15721 aaaagcagga agaagcgatc tctgaagcca cccgtaagct gcgcgaaacg caaaacacgc
    15781 tcaatcaact caataaacag attgatgaga tgaacgcgtc gattgccaaa ctggagcagc
    15841 aaaaagccgc ccaggagcgc agcctcgccg cacaactgga tgccgcattc cgtcagggcg
    15901 agcataccgg tattcagcta attctcagcg gtgaagaaag ccagcgtgga cagcgtttac
    15961 aggcttattt cggctatctc aaccaggcgc gacaagaaac cattgcccag ttgaagcaaa
    16021 cgcgtgaaga agtcaccatg cagcatgctg aactggaaga gaaacaaagc gagcaacaaa
    16081 cgctgttata tgaacagcgc gcccaacagg cgaaactgac tcaggctctg aacgagcgta
    16141 aaaagacgct ggcggggctg gaatcttcca tccagcaagg tcagcagcag ctaagtgaac
    16201 tgcgtgccaa cgaatcccgt ctgcgtaaca gcattgcccg tgctgaagcc gcggcgaaag
    16261 cgcgtgctga acgagaagcg cgtgaggccc aggcggttcg cgagcgccag aaagaagcga
    16321 cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa atcgctgatg tcccgaactg
    16381 gtggcctggg tgcgccgcgt ggtcaggcat tctggccggt tcgcgggccg acgctgcatc
    16441 gctatggcga gcagctacag ggtgaattac gctggaaagg gatggttatc ggtgcctctg
    16501 aaggtactga agttaaagca attgccgacg gtcgagtgat tctggctgac tggctgcaag
    16561 gttacggtct ggtggtggtg gttgagcacg gtaaaggcga catgagtctt tacggctata
    16621 atcagagcgc actggtgagc gttggttcgc aggttcgcgc gggccagcca attgcactgg
    16681 tgggcagcag tggcggtcag ggtcggcctt cactctattt cgaaattcgc cgccagggtc
    16741 aggcggtcaa tccacagccg tggttgggaa gataagtttt gtttccattt cgtcgtaacg
    16801 ttcttgcatt tgccgctctg ttggcgctct cctcccccgt acttgctggc aaacttgcca
    16861 tcgtcattga tgattttggg tatcgcccgc acaacgaaaa ccaggtgctg gcgatgcctt
    16921 ccgctatctc cgtcgctgta ttacccgatt caccgcacgc cagagagatg gcgaccaaag
    16981 cgcataacag cggtcacgaa gtgttgattc atctcccgat ggcaccgttg agtaaacagc
    17041 cgctggagaa aaatacgcta cgcccggaga tgagcagcga cgaaattgag cgcattattc
    17101 gtagtgcggt caataacgtg ccctatgccg tggggatcaa caaccacatg ggcagcaaga
    17161 tgacctctaa cctgtttggt atgcagaaag tgatgcaggc gctggagcgt tacaatcttt
    17221 acttcctcga cagcgtaacc atcggtaata cccaggcgat gcgcgccgcg caaggtactg
    17281 gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc gcagaacgaa gcggacatcc
    17341 gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa cggttcaacc attgccattg
    17401 ggcatcctca cccttcaacg gtacgcgtgc tacaacagat ggtttataac ctgccgccag
    17461 acattacgct ggtgaaagcc agcagcttgc tgaatgaacc gcaggttgat acttctacac
    17521 cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt ccgtggcgtg aagctgtgca
    17581 aaccgaagaa accgatagaa cctgtttatg ctaatcgctt ctttgaagta ttaagcgaaa
    17641 gcatcagcca gagcacgctg atcgtttact tccagcatca gtggcaaggc tggggcaaac
    17701 agcccgaagc ggcgaagttt aacgctagcg caaattaaag gcgctacgcg taatgcgatg
    17761 gtgcgaccgc gttttgtcgc gccattgcca taagcggaaa gaccacagta acgcctggta
    17821 accgaccttc acgctgcgca cattggtaat caggcgctta tacatacccg gaagtaaata
    17881 tctcggcaat catccgttgg cgggtaagaa tatccggctc tttgcg
//
LOCUS       DAJTSF010000081        17644 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000081 DAJTSF010000000
VERSION     DAJTSF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.081"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..671)
                     /gene="chbR"
                     /locus_tag="OO335_002906"
                     /pseudo
     CDS             complement(1..671)
                     /gene="chbR"
                     /locus_tag="OO335_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="OO335_002907"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="OO335_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR6003218.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="OO335_002908"
                     /pseudo
     CDS             complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="OO335_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="OO335_002909"
     CDS             complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="OO335_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR6003219.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="OO335_002910"
     CDS             complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="OO335_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6003220.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3680..4507
                     /gene="nadE"
                     /locus_tag="OO335_002911"
     CDS             3680..4507
                     /gene="nadE"
                     /locus_tag="OO335_002911"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6003221.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4737..5624
                     /gene="cho"
                     /locus_tag="OO335_002912"
     CDS             4737..5624
                     /gene="cho"
                     /locus_tag="OO335_002912"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6003222.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5584..6159)
                     /gene="ves"
                     /locus_tag="OO335_002913"
     CDS             complement(5584..6159)
                     /gene="ves"
                     /locus_tag="OO335_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6003223.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6362..6847)
                     /gene="spy"
                     /locus_tag="OO335_002914"
     CDS             complement(6362..6847)
                     /gene="spy"
                     /locus_tag="OO335_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6003224.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7177..8145)
                     /gene="astE"
                     /locus_tag="OO335_002915"
     CDS             complement(7177..8145)
                     /gene="astE"
                     /locus_tag="OO335_002915"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6003225.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8138..9471)
                     /gene="astB"
                     /locus_tag="OO335_002916"
                     /pseudo
     CDS             complement(8138..9471)
                     /gene="astB"
                     /locus_tag="OO335_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9468..10946)
                     /gene="astD"
                     /locus_tag="OO335_002917"
     CDS             complement(9468..10946)
                     /gene="astD"
                     /locus_tag="OO335_002917"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR6003226.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10943..11977)
                     /gene="astA"
                     /locus_tag="OO335_002918"
     CDS             complement(10943..11977)
                     /gene="astA"
                     /locus_tag="OO335_002918"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6003227.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11974..13194)
                     /gene="astC"
                     /locus_tag="OO335_002919"
                     /pseudo
     CDS             complement(11974..13194)
                     /gene="astC"
                     /locus_tag="OO335_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13640..14446
                     /gene="xthA"
                     /locus_tag="OO335_002920"
     CDS             13640..14446
                     /gene="xthA"
                     /locus_tag="OO335_002920"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6003228.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14613..15323
                     /locus_tag="OO335_002921"
     CDS             14613..15323
                     /locus_tag="OO335_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6003229.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15328..16005
                     /gene="ydjY"
                     /locus_tag="OO335_002922"
     CDS             15328..16005
                     /gene="ydjY"
                     /locus_tag="OO335_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR6003230.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16020..16727
                     /locus_tag="OO335_002923"
     CDS             16020..16727
                     /locus_tag="OO335_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6003231.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16727..17275
                     /locus_tag="OO335_002924"
     CDS             16727..17275
                     /locus_tag="OO335_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6003232.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17285..17611
                     /locus_tag="OO335_002925"
                     /pseudo
     CDS             17285..17611
                     /locus_tag="OO335_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4365 a         4686 c         4308 g         4285 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactacg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatactc tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca
     2461 atttaaatat tacggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa
     2521 ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc
     2581 gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg
     2641 gatttcgggc aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc
     2701 tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc
     2761 tgcctgtgcg cgcatttttg ataccagtaa agagatagac atgcccgcag aacaaaacag
     2821 ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac
     2881 tccgcaagcc ttgacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt
     2941 ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa
     3001 attgtttctc cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga
     3061 agataattaa gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc
     3121 aagctttcag acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg
     3181 tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg
     3241 ctttaccatc acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc
     3301 tcacttcaga cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt
     3361 tgacgtcttt caccacaggc tgtacaaact ggtctttggt acgatcataa gctgtacaac
     3421 ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat
     3481 tccgtcctct tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa
     3541 gcccgtgaac gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt
     3601 cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt
     3661 ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa
     3721 accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct
     3781 gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac
     3841 gcttgccgga aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctcgcaacga
     3901 atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg
     3961 ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc
     4021 ggtattggct agcgagcagg cattgcggga agcaggcatt gaactgagcg attttgtccg
     4081 tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag
     4141 cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata
     4201 tggtgacggc ggtacggata ttaatccgct gtatcgtctc aacaaacgcc agggtaaaca
     4261 gttactgacg gcattaggtt gcccggaaca cctttataag aaagccccaa ctgccgatct
     4321 tgaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat
     4381 cgacgactat ctggaaggga aaaacctacc tgaacaggtc gccagaacaa tagagaactg
     4441 gtatctgaaa accgaacata aacgccgtcc gccaattaac gttttcgatg atttctggaa
     4501 aaagtaataa tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg
     4561 cggcgtgaac gccttattct acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg
     4621 aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt
     4681 ttttaatcgc ctcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg
     4741 tacggcgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg
     4801 aacatttaca ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg
     4861 gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc
     4921 tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct
     4981 ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac
     5041 aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa
     5101 atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc
     5161 tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg
     5221 aacaaaaact ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt
     5281 ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac
     5341 atgcgctacg cttgcgccag tctctggagc gtttgcgagt ggtgtgttgg ccttggcaag
     5401 gtgcggtggc actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact
     5461 ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg
     5521 ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg
     5581 aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag
     5641 tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acacgccctg
     5701 gtcggtagtg agcagtttgt cgcccagttg ccacgccccg ttaatcacaa acaccacccc
     5761 gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac
     5821 atccaggcgc gtcataatat tgaaatccat cgacatctgc cctgccgtca gtttcgcttt
     5881 taccacctgg tccgccgtaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg
     5941 gtctgcgctt tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc
     6001 gggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa
     6061 atcacgtttt gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag cgtttcgcca
     6121 cagattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt
     6181 ttcattgtta tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta
     6241 gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt
     6301 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag
     6361 attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct
     6421 caaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct
     6481 gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg
     6541 cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt
     6601 cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg tggatctgct
     6661 gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact
     6721 tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg
     6781 catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt
     6841 tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc
     6901 gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca
     6961 atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag
     7021 agtgatgaga gaatattaca acacgatgat tttgcagaga ttatgaagaa ctataccgga
     7081 tgactggtga taaataaagc aaataaccag gattaatctg tattaattta taagaaagca
     7141 acttaatacc cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa
     7201 tcccgcgcgt aatcccaacg ctaccaacgg attagggaat aacacatact ctacatcatg
     7261 ggttacggta aaacgttcct ctccgtcctg cgccagcaat gttcctttct caaacggcat
     7321 aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg
     7381 cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag
     7441 cagcgcagca attgcgctgg cagttactgc aaactggcga agatcgtttt gcccaaaggg
     7501 caacgctttg ccaagttcca acgtacaggc cagcgcgcca aaatgtctga cgctgaaatg
     7561 ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg ccgcacccag
     7621 ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaactgcgg
     7681 atgcaaggag ccacgaattg cggtatgtag atcaaggtgc caacgcacag attctttgcc
     7741 ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttctccgct
     7801 ttcagcaaat agctgccaac gaccgccaaa cattcggttc atatcgctat ggcaataacg
     7861 tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc aacgtaacgg
     7921 gatctcgccg tgagatatcg cgccaagcaa cgcgtccagc atttccacag gtgccgtctc
     7981 attaccgtgg atccccgcag aaatcaccag cgcgccttgc ggtggcgtta atggcgtcag
     8041 ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat
     8101 aaccggtttt ttacccgtta aggtcagagc aagaaaatta tccattgccc cctccctcgc
     8161 gctggaacgg ataaaccgaa ccgagattca gtaattggct caatacatcc agtgcttccc
     8221 gcccttcgcg cagcaattgc gggtcggcca gatcggcagc agtaagacga tcgcggtagt
     8281 aacgatccac ccagtcattg agcgcattaa acaaggtatc gttcatcatc accgccggat
     8341 tcaccgcccg gcgttcttct tctgtcaata ccacccgcaa ccgcaggcac gccgggccgc
     8401 cgccattcgc catgctttca cgtaagtcaa agacttttag ttcgctaatc gggttgtcag
     8461 cggcaaggag ttcattgaga taaccccata ctccggcgtg ttcccgacac tcctgaggca
     8521 gcaccaacat catggaacca tcatcgcggc tcaacagttg gctgttaaac agataggtag
     8581 acaccgcatc cgacacggaa acctgagttg ccggaacttc tatcgccata aaaccgttca
     8641 cccgcgtgcc agtaactgtg actggcgagc gaacgcctgt tggtggcaaa acagcacctg
     8701 gcggttactc acggcaatca cgtcattatg aaaaacgctc tggtcgataa cgtccgggtt
     8761 ttgctgggcg aaaatcacct gttggggatt cacctgatta agccttgcca ccgcctcgct
     8821 ggcttcgcga gtctgtcgcg ccggataacg ggaaggccgg gtatcattgc cttcttctcg
     8881 cccgtagaca aaaagtagca tacccggttc accgtaatga ccgccgagac gattgtggtt
     8941 tgccgccccc tcatcaccga gcaacgctac ctgcggcaac gccgaatgga cgctaaattt
     9001 ctcttcgtcc ttaaaaatcg cttttaacag cgattcagtg acgggcgctt ccagcgaacg
     9061 gtgaaattta ttgttcaggt tggcaacggt gagatgcact ttgccatcca gcgtatcggc
     9121 agatggcgcg atcgttgccg cattggctac ccacattggc gaagcggaac tgacgctgga
     9181 aagccagtgc ggtgcctggc gtgcaacttt ttccagtacc tgctcatcgc taccactgaa
     9241 tcccaactga cgcagcaccg gaataaacgg acgctcgtgc ggcggaatca cggcctgggg
     9301 gaatcccgca tcggcaaggg ctttcatttt cagtaagccc tgttttgccg ccagtcgcgg
     9361 gttagacacc tgaaaacggt gacgggtaga ggcttcatta ccaaacgaca ggcccgcgta
     9421 atgatgcgtc agccctacca gcccgtcgaa attaacttcc caggcgttca tcgcaccacc
     9481 tcatcggaaa aatccagccc ggggttaagc gtggcgggca atgttaacga gtccgactcc
     9541 aggctcgcca taggccatgc gcagtaatct gcggcatacc aggcgctggg gcgatggtta
     9601 ccggaagcac caatgccgcc gaatggggcg gtactggcag caccagtaag cggtttgttc
     9661 cagttaacaa tccccgcccg cgcctccagc aacagttgat cgaatttttc ccgctcgggg
     9721 gaaaccagac cgcaaaagag tccgaagcga gtgttgttcg ccatccgaat cgcctcatca
     9781 aaagtatcat aacgccagac gcgcaaaaac ggcccgaaca cctcttcatc cggcacaccg
     9841 ccaacgcccg tcatttcaat gatccctggt gtcagcaacg atgtccctgc ttgtaataag
     9901 cgcggcgcaa gcagggttcg tccgcccatc gcttccagtt gctgccaggc agtaaccacc
     9961 tgctgtgcgg cctgttcaga aatcagcccg ccaataaacg gctgcggttc gtcatcccag
    10021 ttgcccggcg ttaatcgctg gctgacggca accagactag caagaaacgc atcgccctgc
    10081 gctccgcttt tgagcaataa acggcgggcg caggtgcagc gttgcccggc tgtgacaaac
    10141 gccgactgaa tggtcagatg gacagccgcg tcgatatccg ccacctcatc gataattagc
    10201 ggattattac cgcccatctc aagggcgaga attttctccg gctgaccgga gagctggcga
    10261 tgcagctggt agccggtatt ggcgctaccg gtaaacagca aaccgtcgag atcctccagc
    10321 gcactcagcg cctgacccgt ttcacgcccg ccctgcacca ggttcagcac gcccggcggc
    10381 aagccagcct gctgccataa acgcattacc gcttcgccac tccacggtgt cagttcgctg
    10441 ggtttaaaga taatggtgtt acctgccagc aatgccggaa cgatatgtcc gttcggcaaa
    10501 tgaccaggga aattatacgg cccaaacacc gccagcacgc cgtgcgggcg atgtcgcagg
    10561 ctcgccgcac cgtccggcat ttcactacgc tgctcgccgg tacgaacgtg atacgcctta
    10621 attgatatcg cgattttatt gatcatcgcc gtcacttcgg ttgccgcttc ccagcgcggc
    10681 ttacccgttt ctctggcaat aatcgcggtt aattcggctt tattgctttc cagcaatccg
    10741 gcaaagcgtt cgacaacgac ctgacgttca gccaatgaga gccgcgccca gcgcggaaac
    10801 gccgcacggg ctgcccgaca agcctgttcg acctgagcgg catcggcatc attgccttgc
    10861 cataacacct cgcccgatac cggattacgc ttcacacgcg atgcgccctg gcccgttatc
    10921 cagtcaccgt taatccataa agtcatgctg ttttctcctc tgcgcacagg cgcaccagac
    10981 gaacgcgatc ccctgcgtgg catttgaggg catccagttg tgcggcggtt aaaatcaaac
    11041 gctcggttgc cggatcggta cgcgccagca ccacgcggaa atggtgataa ttttcattgg
    11101 cgaccaggca ggccggaaaa tcgccctgcg caggctgccc ttctgccact tccaccagcc
    11161 gacttttacg gatggcgcgc acgcggtcga tgtcacactc aagcgttggc ccgccgtcga
    11221 agatgtcgat atagttgcgg taacgaaaac cttctttctc cagcaccgcg cgggcaggcg
    11281 cggtttgcgg atgtacctga ccgatgacgt cctgggcttc ctgggataaa aagtgggtat
    11341 agatcggatg tttcggcatc agttctgcaa taaatgcctt ttgcccggtg ccgcagagaa
    11401 aatcggcgcg gctaaaatcc atcgaaaaga agcgtttacc gaggctttgc cagaacggtg
    11461 aatagccgtg ttcgtcaatc accccgcgca tttcagcaac cactttgtca ttaaacttgt
    11521 cgcgaaaagc cgccataaac ataaagcgcg atttcgacag caaatagccg ttgccctctt
    11581 tgcgccagtc cgggtcgaga aacagcgtgc acagctcgct gctgccggtg tgatcgttac
    11641 taagaaacag cgtcggcaat gcgttataga cattcagctc ttttgaggcg tgaaccaacg
    11701 tgccgacgcg atagttgtac cagggatcgt tcagcccaac cgccacctca atagcgcaaa
    11761 tccccgccac ggtgcctgtc tcgctatctt ccagcacgaa cacatagccc tgctcgcttt
    11821 tgggcagttc accttgccag gttttgattg ccctttcgat acgcgccgaa agcgtggctt
    11881 cattggcggg aagcgacgtc aggccgccgc ccgttttgct ggcaagctgc atcagcgccg
    11941 agacatctga gcgctcaacg ggacggatga ccatcatgat gaacctcggc taacaaagtg
    12001 ttcgcaagca gctgcaaagc gatccagtcc ggtcgtcacc tcttcttcgc tgacattgag
    12061 cgcaggcgca aaacgcacca cgttgccacc cgcaatcagt accatcacgc ctgctttcgc
    12121 cgcttccaga gagatctgtt tcgcttgccc ggcgtaatcg gcattcagta cacagccaat
    12181 caacaaacct aagccgcgaa cttcactgaa caaaccatag cggtgattaa tagtattaag
    12241 acgctcaaca aaccagtcgt gacgctgttt aacgccatta agcatctctg gtgtgttgat
    12301 gagctccagc actttgcctg ctaccgccga ggccagcgga ttaccgccat aggtggtgcc
    12361 atgagtgcca acggtcatca cgcgggcgca ctcttcggtt gccaacaacg caccgaccgg
    12421 gaaaccgccg cccagcgctt tggcggtggt taacagatcg ggcgtcacgc cgtagtgcat
    12481 ataggcatac agctcaccag tgcgcccgac gccggtttgt acctcatcaa aaatcagcaa
    12541 ggcattgtgg cggtcacaca attcacgcag accttgtaaa aacgcgttgc tggctggcac
    12601 cacaccgcct tcgccctaga tgggctcgac aatcaccgca caggtggcgt cgtcaatcag
    12661 cgcgctggca gagttaatat cgttatatgc agcatgacga atatccgccg gcagtggcgc
    12721 aaaatcctgt gaataggctg gctgcccacc cgcactgaca gtaaacagcg tgcgaccatg
    12781 aaacgcattt ttgaacgcca cgatgccact cttatggctg ccgtagcggt cgtgagcgaa
    12841 tttacgcgcc agttttagcg ccgcttcgtt ggcttccgca ccggagttac aaaagaagac
    12901 gcgatcggca aacgtggcgt cgatcaattt tttcgccagt cgcagtaccg gctcgttggt
    12961 gtaaccgttg ccggtatgcc agaacttact cgcctgttcg ttcagcgctt cacgcagttc
    13021 cggatgcgca tggcccagcg cgttcaccgc aatgccaccc gcgaagtcga tatactcttt
    13081 cccctgctga tcccacaagc gcgaaccttc gccacgtacc ggtataaagg gtgccggagc
    13141 gtaaacaggt atcatccatt catcaaagtt ttcacgcgta attggctgag acatagcgac
    13201 ctctacagta aataattcgt tatttgtatg ttaacaataa gtaatgtttg cgctgtaaat
    13261 gtagattgca gggttcgtgc cagccagtga taaaagtgca taaacggcgg aggctaactg
    13321 gaaatcaagg agttataacc aaaccatatg catttaaagt gcatataaag tgaatacgtt
    13381 tgcgatgtgg gtgaataaaa agaataaaaa acgcaatgtt atgcagaagt aaaatataat
    13441 tctagaattg tgatcattga cgaaatttac tggaaattac tgcgccattc tgacgcagcg
    13501 cgcaccaaaa gcgggcattt tttgcgccat cgttgacatc attaacaacc atcgatcaaa
    13561 tcacttaaca acaggcggta agcaacgcga aattctgcta ccatccacgc actctttatc
    13621 tgaataaatg gcagcgacta tgaaatttgt ctcttttaat atcaacggcc tgcgcgccag
    13681 acctcaccag cttgaagcca tcgtcgaaaa gcaccaaccg gatgtgattg gcctgcagga
    13741 gacaaaagtt catgacgata tgtttccgct cgaagaggtg gcgaagctcg gctacaacgt
    13801 gttttatcac gggcagaaag gccattatgg cgtggcgctg ctgaccaaag agacgccgat
    13861 tgccgtgcgt cgcggctttc ccggtgacga cgaagaggcg cagcggcgga ttattatggc
    13921 ggaaatcccc tcactgctgg gtaatgtcac cgtgatcaac ggttacttcc cgcagggtga
    13981 aagccgcgac catccgataa aattcccggc aaaagcgcag ttttatcaga atctgcaaaa
    14041 ctacctggaa accgaactca aacgtgataa tccggtactg attatgggcg atatgaatat
    14101 cagccctaca gatctggata tcggcattgg cgaagaaaac cgtaagcgct ggctgcgtac
    14161 cggtaaatgc tctttcctgc cggaagagcg cgaatggatg gacaggctga tgagctgggg
    14221 ggtggtcgat accttccgcc atgctaatcc gcaaacagca gatcgtttct catggtttga
    14281 ttaccgctca aaaggttttg acgataaccg tggtctgcgc atcgacctgc tgctcgccag
    14341 ccaaccgctg gcagaatgtt gcgtagaaac cggcatcgac tatgaaattc gcagcatgga
    14401 aaaaccgtcc gatcacgccc ccgtctgggc gaccttccgc cgctaattta gcagctctcc
    14461 tggctcaaac tgggtcagga gaattaacct tgagaaaaat caacaaactg tcagtaatga
    14521 tttgttgcct gccgtccttt gttataccgt cactgcgttt ttagttgtct gaccacttct
    14581 ctattatcaa gtttgatata ggaaactcca cgatgaacgc tgagcgtaac tttctgtttg
    14641 cctgtcttat ttttgcgctg gtcatttacg ctatccacgc tttcggttta ttcgatctgc
    14701 tcaccgattt accccactta cagacactca ttcgccagag cggattgttc ggttatagcc
    14761 tctatattct gttattcatc aatgcccccc tctttctgtt accaggaagc atactggtga
    14821 tcgctggagg aatagttttt ggcccgctct tagggacaca actctcatta attgccgcaa
    14881 cgctggcctc ctcgtgctca ttcctgctgg cgcgctggct gggacgtgat ttactgctga
    14941 aatacgtggg ccatagccat accttccagg ccattgaaaa aggcattgcg cgtaacggta
    15001 ttgattttct tattctgacc cgcttaatcc cgttgtttcc ttacaatatt caaaattacg
    15061 cttacggatt caccaccatc gccttctggc cttataccct tatttcggca ctcacgaccc
    15121 tgcccggtat tgttatttat accgtgatgg caagcgatct cgccaatgaa ggcattacgc
    15181 tgcgctttat tttacaactc tgtctggcgg gcctggcgct gtttattctc atccagctcg
    15241 caaaactcta cgcccgacac aaacatgtgg atctgtctgc ttcgcgccgc agcccactta
    15301 ctcacccaaa aaatgaagga tagaacgatg tcgcaacatt attcagtgtc atggaaaaaa
    15361 ggactggctg cactctgctt actggctgtt gccgggctta gcggctgcga tcaaaaagag
    15421 aatgcggcgg caaaagtgga atacgacgga ctttcgaaca gccaaccgct gcgcgtcgat
    15481 gccaataacc atacggtaac catgctggtg caaattaatg gtcgtttcct caccgacgac
    15541 actcgtcacg gtattgtgtt taaagatggc tccaacggac ataaatcgct gtttatggct
    15601 tatgcgcccc cgaaagcatt ttatgaagcc ctgaaagagg caggtggtac gccgggcgaa
    15661 aacatgacga tggataataa agaaacgact catgtcacag gcagcaaact ggatatttcg
    15721 gtcaactggc aaggggcggc aaaagcgtat tccttcgatg aagtgattgt tgacagtaat
    15781 ggcaagaaac tggacatgcg ctttggcggt aatttaacgg cagcagaaga gaagaaaacg
    15841 ggttgcctgg tgtgtctgga tagctgcccg gtcggcatcg tcagcaatgc aacatacact
    15901 tatggtgcgg tggaaaaaca cggcgaagtg aaattcaaag gcaatgcctc agttctcccg
    15961 gcggataaca cgctggcaac ggttaccttt aaaatcaccg aataaagcca agataaagga
    16021 tgatgaagat acaatcgcgt aaaatctggt actaccgtat aaccctcatc atcctgttgt
    16081 tcgctatgct gctcgcatgg gcgctgcttc ccggcgtcca tgagtttatc aatcgcagcg
    16141 ttgcggcgtt tgccgccgtg gaccaacagg gtatagaacg ctttattcag tcttacggcg
    16201 cactggcggc agttgtctcg ttcttgttga tgattttaca ggccattgct gcaccgctac
    16261 ctgcgttttt gatcaccttt gccaatgcgt cgctgtttgg cgcgttctgg ggcggcttgc
    16321 tgtcgtggac cagttcgatg gccggcgcgg cgctgtgctt ttttatcgcc agagtgatgg
    16381 gccgcgaagt ggtggaaaaa ttaaccggca aaaccgtgct tgacagtatg gacggctttt
    16441 tcactcgcta cggcaaacac accatcctgg tctgtcggtt attgcctttt gtccctttcg
    16501 atccaatcag ctatgctgcc ggtctgactt caatacgttt tcgctcgttt tttatcgcca
    16561 ccgggcttgg tcaattaccg gcgactattg tttattcctg ggcaggcagc atgttaacag
    16621 gcggtacttt ctggtttgtc accgaactgt ttattctgtt tgccctgacc gtggtgattt
    16681 ttatggcgaa gaaaatatgg cttgagcgcc agaagaggaa tgactgatgg gtttaccgcc
    16741 gcttagcaaa attcctttca ttttacgtcc gcaggcgtgg ctgcatcgtc gccattacgg
    16801 cgaggtgcta agcccgattc gctggtgggg gcggatcccg tttatctttt atttggtgtc
    16861 aatgtttgtt gggtggctgg agcgcaaacg ctcaccgctc gatccggtag tgcgatcgct
    16921 tgtcagcgcg cgcattgcgc aaatgtgcct gtgtgagttt tgcgtggata tcaccagtat
    16981 gaaagtcgcc gagcgtaccg gcagcaccga taaactgctg gcagtggctg actggcggca
    17041 aagcccgctc tttagcgatg aagaacggct ggcgctggag tacgccgaag ccgcaagcgt
    17101 cacaccgcca acagtcgatg atgccctgcg tacccgactg gctgcgcatt ttgacgctca
    17161 ggcgctcacc gaactgacgg cattgatcgg cctgcaaaac ctgtcggctc gttttaattc
    17221 tgccatggac attcccgctc aggggctgtg ccgtattccg gaaaaacgtt cttaaggagg
    17281 gaagatgcgc cattgtgggt ggttgctggg attgttatcg ctgttttctt tggcaacaca
    17341 tgccagtgac tggcaggaaa ttaaaaatga ggccaaaggg caaaccgtct ggtttaacgc
    17401 ctggggcggc gataccgcaa ttaaccgcta tctcgactgg gttagcggcg agatgaaaac
    17461 ccattacgct ataaacctga agattgttcg tctcgcggat gccgcagacg cggtgaagcg
    17521 cattcagacc gaagctgctg ccggtcgtaa aacgggcggc tcggtggatc tgctctgggt
    17581 gaacggcgaa aacttccgca ccttaaaaga gtaatgccag tcagttaagc aactgactgg
    17641 catt
//
LOCUS       DAJTSF010000082        17385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000082 DAJTSF010000000
VERSION     DAJTSF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.082"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(41..772)
                     /gene="rlmB"
                     /locus_tag="OO335_002926"
     CDS             complement(41..772)
                     /gene="rlmB"
                     /locus_tag="OO335_002926"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6003233.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(952..3393)
                     /gene="rnr"
                     /locus_tag="OO335_002927"
     CDS             complement(952..3393)
                     /gene="rnr"
                     /locus_tag="OO335_002927"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6003234.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="OO335_002928"
     CDS             complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="OO335_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6003235.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4062..5360)
                     /gene="purA"
                     /locus_tag="OO335_002929"
     CDS             complement(4062..5360)
                     /gene="purA"
                     /locus_tag="OO335_002929"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6003236.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5464..5661)
                     /locus_tag="OO335_002930"
     CDS             complement(5464..5661)
                     /locus_tag="OO335_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6003237.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="OO335_002931"
     CDS             complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="OO335_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6003238.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="OO335_002932"
     CDS             complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="OO335_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6003239.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="OO335_002933"
     CDS             complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="OO335_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6003240.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="OO335_002934"
     CDS             complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="OO335_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6003241.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="OO335_002935"
     CDS             complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="OO335_002935"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6003242.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="OO335_002936"
     CDS             complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="OO335_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6003243.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="OO335_002937"
     CDS             complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="OO335_002937"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6003244.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="OO335_002938"
     CDS             complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="OO335_002938"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6003245.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="OO335_002939"
     CDS             complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="OO335_002939"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6003246.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15980..17119
                     /gene="queG"
                     /locus_tag="OO335_002940"
     CDS             15980..17119
                     /gene="queG"
                     /locus_tag="OO335_002940"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6003247.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17102..17155)
                     /locus_tag="OO335_002941"
     CDS             complement(17102..17155)
                     /locus_tag="OO335_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003248.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3942 a         4959 c         4367 g         4117 t
ORIGIN      
        1 aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgaaccg
       61 cttcaaataa gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca
      121 ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt
      181 ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg
      241 cctcgcctgc cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg
      301 caaggttagt cacccgaatc agcggaacgc tttctgccgc gccgcaggct acttttttcg
      361 ccgtagcgtt gagctgtgcg gagcgatctt tcggcacaat caccgcatga acgcctgcgg
      421 cgtccgcact acgcaggcac gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca
      481 gcaggaacgg ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac
      541 gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga
      601 gatattggcg gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg
      661 gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg
      721 cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac
      781 tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata
      841 tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac
      901 aaagcgcatt ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc
      961 cacttttttc ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc
     1021 cgatggcttt ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc
     1081 accgcggaag gcgctgtctg gctcaaagtt taccttttta ccgacctgac aacgcttgcc
     1141 gccttttttg cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg
     1201 cggtgcgcgt tcactggaga tcagactaaa gtcgattttg cgctcgtcca tattaaccgc
     1261 ttcgacgcga acttccacgc gatcgcccag gcgataagtc tggccgctgg attcccccat
     1321 caggcgttgc cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac
     1381 cagaccatca atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga
     1441 aattacgcct ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc
     1501 agcgacatcg cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag
     1561 ttgcaacatc tcttccatcg aataatggta gccgccggtt tcagtggtgt tgccctgatg
     1621 cccctgctct ttcgccagca gatatttaat ggcgcggtgc agcgtcaggt caggataacg
     1681 acgaatcggc gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt
     1741 ttctggatcg taaatcgcct gtttcatcga gcgtagcagc atggtttgca gcatttctgc
     1801 atcaggacgg tcggcaaccg actccagcag ttccgcgtag tcacgcggtt ccggcttgtt
     1861 accacccggc agctccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc
     1921 ggtgctcggc ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg
     1981 cgccgccgag atattcgcca gaatcatgca ctcttcaatt aatttgtgcg cgtcgttacg
     2041 ctgggtctgt tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc
     2101 gctctcaaat gagatcccac cgcgttcttc acgggcttta tccagcactt tatagaggtt
     2161 atgcaactct tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc
     2221 ctgcagaata tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc
     2281 gtagaatttg tagcccgtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca
     2341 caggcggtct acctgcgggt tgagcgaaca caggccgtta gagagcactt ccggcagcat
     2401 cgggataacc tgcgaaggga agtacaccga cgtgccacgg ttacgcgctt ctctgtccag
     2461 cggcgttggc ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc
     2521 gccgccgcgt tttttctcgc agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc
     2581 aatggtgacc agcggtaaat cgcgcaaatc gacacggccc gcttttgctt cttccggcac
     2641 ttcttctttc agccccgcaa cctgttgctc aacagcctgc ggccagatat acggaatttc
     2701 atgggtacgc agagcgatat caaccgccat gccggtgccc atattgtcgc ccagcacttc
     2761 gacgatttta cccaccgctt tggtgcggcg agtcggacgc tgagtcagtt cgactacgac
     2821 cacaaagccc atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag
     2881 acggctatcg tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat
     2941 ctggctggtt tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc
     3001 agcacccagc ggctgcgcca gcacctgatc gccatgaatg caggttttca tctgctcgct
     3061 ggagagatac aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg
     3121 accaataacg gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg
     3181 agtgaagacc agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag
     3241 ctgctcttcg ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc
     3301 acgtttggtt aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc
     3361 agcttcgcgt tcctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat
     3421 gaagattttc gtcactccac cagcaataat ttataaagcg gttgattctc ttcaaccaaa
     3481 tcggcaagcg tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc
     3541 tgtttcaacc gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat
     3601 aagggctcca gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg
     3661 cgaatgccgc cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg
     3721 attattttga ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg
     3781 gtcatccgcc cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg
     3841 aaactcgtta actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg
     3901 atgataaacc agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc
     3961 gaggatttaa cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac
     4021 tgttagcccg gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacgggtcg
     4081 cgcagaatca tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacaccg
     4141 gtcagctctt caatacgctt gatgtagttc agcgccgcct gcggcaggcc gctacgatct
     4201 ttcacgccga aagtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct
     4261 ttccagtcgt cagctgccag cggagtggta gtcacttcgc gaccatccgg catacggtaa
     4321 gccacgcaga gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag
     4381 ccagacaggg agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta
     4441 cgacgacgac gacccgtagt tgcaccgaat tcgttaccct gcttgcagag gaactcgcca
     4501 gtttcatcaa acagttcggc cgggaaagga cctgcaccta cacgagttga gtaagctttg
     4561 aggataccca gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca
     4621 ccagcagtgg tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc
     4681 gtaccctgcg caccttcgaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg
     4741 tcagaaacgt caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga
     4801 actttctggt aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat
     4861 tccatcactt ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc
     4921 agaccgcgac gtgccacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg
     4981 atcgctttcg cgccacgcgc tttctcacgc gcgttatcca gagcaacgtg ataatcaagg
     5041 atcagcggac atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc
     5101 agttctttca tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg
     5161 ctggttacat tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg
     5221 ttgattacga gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc
     5281 cgttcagtca gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt
     5341 acgacgacgt tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat
     5401 cgctttttca gagttacagc actttttgca ctcaaaaatg acctcgatca gtctgttttt
     5461 tgttcagcca atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc
     5521 accgccaaaa cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt
     5581 ccatgccttc gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc
     5641 cagccagatt gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct
     5701 gaggatgcgg tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt
     5761 cggcgtcttc atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt
     5821 gccagagaag ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc
     5881 tttactgaat gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcataat
     5941 gcggccctga cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg
     6001 cagtttttcc gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc
     6061 ggcgcgcata cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat
     6121 acgcacatcg acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac
     6181 tttgcccttc gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc
     6241 cggggtagta acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc
     6301 gagggtcaga cgaccacggg aatcggtgac gatatctttc acgtccaggc gaccaatttc
     6361 agaacgcaga cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc
     6421 accacccgtt gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc
     6481 gacgatcagg tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg
     6541 aatacgtgcg tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagaccagg
     6601 ctcataaacc aaaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat
     6661 accgcgctca ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat
     6721 gataatcgcg ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc
     6781 tgcgcgttgg cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt
     6841 gctccactgg ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta
     6901 tcgctcttcg ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc
     6961 aggttgccac ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc
     7021 gtctcgatat acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt
     7081 ttagcaaagc gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc
     7141 gcctcttcga ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggaatac
     7201 gcttctgctt cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac
     7261 gccgctttta cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata
     7321 cccatgtcat acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg
     7381 gtacgacctt ccgtcagaat gcggtccatg gtgtatttac cgataactcc acgcagggcg
     7441 ctgtcggtag cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc
     7501 ggattggtga cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc
     7561 agcatcacac cagaagcggc cagttcacgc acggcttcca cgttcaccgg tttgacttcg
     7621 tcgataaacg tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag
     7681 cgtgttacca cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata
     7741 atgacaatcg ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct
     7801 tgcgatgaac tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt
     7861 ttgctcagtt tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg
     7921 tttccatttc cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt
     7981 ccgttattac cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc
     8041 cctgttgtct ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat
     8101 caggtaatcg atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat
     8161 acgaacttgc agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag
     8221 ctgataaaaa cgactcctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc
     8281 cacctcgccg gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt
     8341 ctgtgcggaa agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc
     8401 gaaatcttcc agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc
     8461 gatctcttca agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc
     8521 atcaatgacg tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc
     8581 cgccaccaga tcgtgcggca ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc
     8641 accgacatct gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc
     8701 tgccgcgtag acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt
     8761 atatcccacc agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc
     8821 acgctgcttt tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa
     8881 acgacggtcg gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg
     8941 tctttcaagg tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag
     9001 ctcaacctgc aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag
     9061 gccggtgcgg tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg
     9121 gctcagggca tggtcaaaaa gaacgaccga agcacccatc gctttgacag cttccgcaat
     9181 ttcaactgct ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt
     9241 aatcacctgc aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc
     9301 ttccatatct ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc
     9361 ataacggtca aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg
     9421 gctccccgtg taaaaaaaca gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc
     9481 ggaagtattc tgcgcgctgc taccatgatg gtagttactg ctggtaccgc caccggcgtt
     9541 gttactgtga tgagaaaccg ggcgagatgg gacaacagta gaaatcgcgt gcttgtaaac
     9601 catctggctg accgtgtttt tcaacaggat cacgaactga tcaaaagact cgatttgccc
     9661 ttgcagctta ataccattca ccaaataaat agaaactgga acacgttccc gacgcagtgc
     9721 gttcaggaac ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct
     9781 tatttgtact ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc tcaattgtac
     9841 acattcagcc tgcgatagca ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt
     9901 tttcactgtc aagccagtga accccttccc aaccacgcag ccaggttatc tgccgcttcg
     9961 ccaactgtct cgtggcgcaa acacctcgat aaaccatttc atcgtatgag atttcgcctt
    10021 caaggtaaga ccacatctgg cgataaccca cgcaacgaat ggaaggcaag tccgtatgca
    10081 aatctcctcg ggcaaaaagc gcccggactt ctgcttcaaa acctgaagcc aacatctgat
    10141 gaaaacgctg ctcaatgcgt tgatggagca gttcacggct cgccggggcg atggcgaact
    10201 gatgcacctg atacggtaga gcgtctcctg acgtttgcgt cagttccgtt aaagttttac
    10261 ccgaaatgaa aaaaacttcc agtgcccggg aaagcctttg tggatcattt ggatgaatcc
    10321 ttgctgccgc aaccggatct acctcctgaa gttgacgatg caatgactcc caaccttgct
    10381 ctgccgcctg ttgctcaatt ctggcccgca cttccgggtc tgccgacggt agcggcgaca
    10441 acccttccag caatgccttg aaatacaaca tcgtaccgcc cactaacagt gggatccgcc
    10501 ccgccgcggt gatatcggcc atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt
    10561 aagcctgcga cggatcgcga atatccagca atcggtgcgg cgcggcgagt aactcttcag
    10621 cgttcggctt cgccgtcccg atatccatcc ctttgtaaat aaggacagaa tcaacgctta
    10681 tcaactctac tggtaaaatt ttacgcagct caatggctaa cgccgtttta ccggaggccg
    10741 tcggccccat caaaaaaatc gccttaggca ggctcgcctt actgatatca ctcatctttc
    10801 agggctttta tcgccggatg taaatcaaca gattgtaaca gaccacccgg cggcgttttc
    10861 acaagttgcg gacataaccg ttccacgtcc gccagcaggg ttatggcctg tgccattgac
    10921 cactgcgcat gttcgctcat cagatttcgt gcaatccact gcgcaatatt gccaggttcg
    10981 aatacggact gcttcgccag gtagcctatc agttcaggaa tcaagatttg taaattttgt
    11041 tggcgtaagg gtaaaggcac ggccctgatg gtcacatgct gtgcatctga ctggaaatca
    11101 atacccaatt ccgctagggc agactgcgct ttttctaatg ccgatttttc ttcgccagaa
    11161 acttttagcc gcaacggaat cagcagcggc tgggcgcaaa cgggcgcttc acccggcgtc
    11221 aattgtgcct gacgcagcca acgttctgcc actggcaagg ataaaagtga aatgttgcca
    11281 tcgcgctcca gcaacgcaca gtcggaatgg acgatagtca gtacccgacc aaaactctga
    11341 ctgttcgccg caagtgcagg ttcctgcggt tccggcgctt ttggtttttg catcggcgcg
    11401 ggcgtttgca aaagctggcg atacacttca ccttgctgtt tctggtagcc tggctgcgca
    11461 ttcggccagg gggcagccgg acgactgcct gaggcgggcg caggagagta gcgcggagct
    11521 accggctcac gagctgccgg ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc
    11581 gggatggcac gcggtgcggg ttgtggttca tcgtccagcg gtagcggcgt ttccagttgc
    11641 tgttgtagca cgctcagcac gccctgatag ataaaatcat gcaccagacg cgactgatgg
    11701 aaacgcactt cgtgtttggc ggggtgtacg ttgacgtcca cctgatgcgg gtcgatctcc
    11761 agatacaaca caaatgccgg ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg
    11821 atcgcatgat tgatcaggcg atcgcgcatc atgcgaccat tcacgtagca atactgaatt
    11881 tccgccagtg cgggcgtagt gtgatttgga tcggccaccc agccgcgcag ggtgagatcg
    11941 ccgtgttgcc attcaatcgc cagcgcttgt tcgagaaaag cggtgccgca aatcgcgcct
    12001 aagcgccgtt ctttttgccc gccttccggc actgcgcggt actgacgcac aattttaccg
    12061 ttatgcgaca ggttgatcgt gacgtcgaaa cgcgccagcg caatgcggcg gatgatttca
    12121 tcaatatggc taaattcggt tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg
    12181 tagaacagat ccagcacctc cagcgtcgtt cccaccggat gcgctgccgg tttaaccgtc
    12241 acgtccatgt cgcgtccttc ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt
    12301 gaagtgagcg tcaggcggga aaccgaactg atactcgcca gcgcctcacc gcgaaagccc
    12361 aggctgataa tggcttcgag atcgtccaga gaggcgattt tactggtggc atgacgagcc
    12421 agcgccagcg ccagctcatc ttttttgata ccgcagccgt tatcacgaat gcggataagt
    12481 ttcgccccac cgcgttcgat atcaatatcg atacgcgtcg cacctgcatc gaggctgttt
    12541 tccactagtt ctttgactac cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg
    12601 ttcgccagtt gtggcggtaa gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg
    12661 cgatctggcg tcgtcaccgt actggcagtt tgtgccgttg caccctgcgg cgcagattgc
    12721 atcggatgcg caaggaagta attgcgcagg cctttgtaaa tggcttctgc cagctgttgt
    12781 tggtaatcgt cgctcgccag caaacgttct tcgctgttgt tgctgataaa accggtttcg
    12841 accagtactg atgggatatc cggcgagcgc agaacgccaa ggctggcgtg ttctggtcga
    12901 cgtttatgta tttcgccaat gcgttgcaac tgactgatca tactggtcgc tacatcatac
    12961 cctacccgct gggaatgacc gaactgtaaa tccagcaccg cctggcttaa ataggggtca
    13021 gactgactgt tcgccagcac atcacccgcc ccacccagca gctccgacag tttctcgtgc
    13081 tgctccagcc agctggccat ttcactgttg gcgcgacggt tagagagcac ccatacggaa
    13141 gcgccagtcg cactacggtt cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg
    13201 ttttgcttat gtgccacatc actgcgcccc atcaccgaga taaagtaatc cccgtcacgg
    13261 gttaaaacgc ctttaaacat cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg
    13321 gcgatggtga catttttctc ccgcgtacca ccggggccga tagcgccagg gtcctgaccg
    13381 ccgtgtccgg catcaatagc gatgataatt ttatcgccag tgttagccgt cgcgcgcgct
    13441 gccggacgcg ttaccgtatt actgctgata acacccgtag tgcggttact ttccgtttta
    13501 aacggattgc gcgccggttc gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt
    13561 ttcgcaacca cgggcggcgg aggaggcggt ggcggcacat cggcgttaat cgtaaagacg
    13621 acagtgtaat tgctgccatt ctgccgcttc accgcttcgg ttttaccgtt ttcggtaaga
    13681 tcgaccacca gccgtagcgt ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc
    13741 agattattgc cgctgaacaa caacggcagt ccctgaatca tgcccgtttg tttgatatcg
    13801 agcgccacgg tgcgtttgct ttgatggcta aacgcataat caggatcgcc aataaaactc
    13861 aacgttatcc gcgcctgttg attaccgtta gaaacctgaa tatcagagag cgtcgcggca
    13921 cccaccggcg tgcacagcag cagcagcgtc gctaccaacc aatttctgat gcgatacatc
    13981 atcccgccac ctttcaaagg ttaaccggct aaacgcgcca gcaacaattc acccgcagag
    14041 gaaaccgcac tcacgcgcgc ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca
    14101 tccgggtcag gaagaacacc tgtaccttgt tgtggccact ccaccaggca gatggcatcg
    14161 ttggcaaaat aatcgcggat ccccataaac tccagctcct cgggatcggc aaggcggtac
    14221 aaatcaaagt gatagaccat taagttgtcg agcgtatagg gttcgaccag cgtataagtg
    14281 gggcttttga cattaccctg atgacccaga gcctgtaaaa agccccggct aaaggtggtt
    14341 ttacctgcgc ctaaatcgcc atacagatag attacggttg cgccatcgca ggctttcgct
    14401 acccgctcgc ccaggtctaa tgttgcctgc tcatcaggga gcggaattac tcgattcatc
    14461 atggttttta tcagtcactt ccgggttaac aatacgctgt agcgtggaaa agagatcggt
    14521 tgccagcatc ccgcgcgttc caaaacgcgc cgccagtacg tcagctgccg caccgtgcgc
    14581 gacacagcct gcacaggctg catcatacgg cgacagtttt tgcccaagca atgcgccaat
    14641 aataccagag agcacatcgc ccatgccgcc gctcgccatg cctgcatttc cggcatcaat
    14701 aatgcctaaa gcgtcaggat gggcggcgac cacggttccg gcacctttca gcaccgctac
    14761 gccgccataa cgttgtacca gacgtttggc gcaatgtaag cggtcacttt caatttcagc
    14821 gacggaacag cctaacaacc gtgcggcctc gctaggatgc ggcgtgatca cgcgattgtg
    14881 acgcttatcg ggattgattg ccagcaggtt caatgcatcg gcatcccaca acatcggttt
    14941 gcgaaaattc tcaacttttt gcagggcttt tttcccccac tcttgctggc ccagaccggg
    15001 accaatcacc accacatcgg cccattccag gctttcggta agagagtcca tagtcagttc
    15061 atgcaccatc aattctggtc gtgcagtcag cagcggcgca atgttctcac tgcgggtcag
    15121 tactcggact aaaccagcac cagcacgtag cgccgcttcc cccgtcatac gaatagcccc
    15181 cgccgtgccg tgatcgccac cgataatcac cagccgcccg tgatcgcctt tatgcgaagt
    15241 cgggcgacgc ggtttcagcc agtgagaaag ttgttctgcc gaaaaccgct gaattttcgt
    15301 ctcctgacct gccagccagc tatccagccc cagtgagtca aaatgcagtt gtcctgtaac
    15361 atcccgcgct tttccagtga gcaagcctag tttcagcgca ataaaagtga tggtgtgatc
    15421 ggcgttgatc actgcgcctg gcgtagcgcc ggtttcagcc agcaggccgg aagggatatc
    15481 aaccgccaca atcggcgcag gatgggaatt agcgtggtcg attaactggc taatggattc
    15541 gcggggcgct tgctgcaagc cggtaccgag cagcgcatca acaatcagat ctaccgattc
    15601 gggccagaca atattcgaag catggatctc gccacccgcg tttaaccatg cttcgcgtgc
    15661 cagcgcggcc tcttccggca acggtttgtc gctctcctgg gccaacaacg tgacctcaat
    15721 gccgaccgct ttggccagtc gcgcgaccac gtagccatcg ccgccgttat taccatgacc
    15781 gcacagcacc agccagtggc gggcgtcagg atacgccgaa cgacacacct ggaatgcggc
    15841 ctcgccagcg cgaagcatca gctcatagag tgtgagcccc agcacatctg ccgcctcgcg
    15901 ttctccgcgg cggatatcgt cggcgtacca gacggtgtgt ggtatactta cggggttttt
    15961 cttcattgta tggtccgtca tgtcagagcc cctcgatctc aatcagttag cgcaaaaaat
    16021 taaacagtgg gggctggaac tgggctttca gcaggtaggt attaccgata ccgatctcag
    16081 cgagtccgag cccaaactgc aagcatggct ggacaaacaa taccacggcg aaatggactg
    16141 gatggcacgt cacggtatgc tgcgcgctcg cccccatgag ttattacccg gtacgctgcg
    16201 tgtaatcagc gtgcggatga attaccttcc tgctaacgcc gcatttgcca gcacgctgaa
    16261 aaaccccaaa ctcggctatg ttagccgtta tgcgctgggc cgtgactatc acaaacttct
    16321 gcgcaaccga ctcaaaaagc tgggcgagat gattcagcaa cattgtgttt cgctgaattt
    16381 tagaccgttt gtcgattctg cgcctattct cgagcgcccg ttagctgaaa aagctgggct
    16441 cggctggaca ggtaagcact cacttatcct caatcgcgag gccggttcgt tcttcttttt
    16501 aggcgaattg ctggtcgata ttccgctgcc cgtggatcaa ccagtcgagg aaggatgcgg
    16561 caaatgcgtg gcctgtatga cgatttgccc gaccggtgcc atcgtcgagc catataccgt
    16621 cgatgctcgc cgctgtatct cttatctcac catcgaactt gaaggggcgc tcccggaaga
    16681 gttgcgaccg ttaatgggaa accgtattta cggttgcgat gactgccagc ttatctgccc
    16741 gtggaatcgc tattcgcaac tcactacaga agacgatttc agcccgcgta agccgctaca
    16801 cgcaccggaa ctcattgagt tatttgcctg gagcgaagag aagtttttaa aagtcacgga
    16861 aggatcggcg attcgccgta taggtcacct gcgctggctg cgtaatatcg ccgtggcatt
    16921 aggcaatgcc ccttgggatg aaacgatttt gacagcgctg gaaagtcgta aaggtgagca
    16981 cccacttctc gatgagcaca tagcgtgggc gattgcgcag caaatagaga gacgaaatgc
    17041 gtgcatagtc gaagtgcaat tgccgaaaaa acagcgtctg gttcgggtga ttgaaaaagg
    17101 gttaccgcgt gacgcctgat tcatccacag cttgtgaata aaaataaaaa cgcattgcaa
    17161 ttcaagacga ataaaatcgt caagtgatcg actcgacaaa acggaatctt gatttttaac
    17221 ttatttatca ataggttaaa aaaataccat tcaccgtgcg aagtttcttt gaaattaaga
    17281 ttacagcctg aggctgtgga taactctgtt tacaagagtt tgtcagagac aacaaaaacc
    17341 agcaataacg catcgcgtcg ctgtagatat tttattgaga gaaga
//
LOCUS       DAJTSF010000083        17265 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000083 DAJTSF010000000
VERSION     DAJTSF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.083"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            445..1011
                     /gene="mntP"
                     /locus_tag="OO335_002942"
     CDS             445..1011
                     /gene="mntP"
                     /locus_tag="OO335_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6003249.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="OO335_002943"
     CDS             complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="OO335_002943"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6003250.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="OO335_002944"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="OO335_002944"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6003251.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2205..2348)
                     /locus_tag="OO335_002945"
     CDS             complement(2205..2348)
                     /locus_tag="OO335_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6003252.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(3019..3306)
                     /locus_tag="OO335_002946"
     CDS             complement(3019..3306)
                     /locus_tag="OO335_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR6003253.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="OO335_002947"
     CDS             complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="OO335_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR6003254.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3683..3922
                     /locus_tag="OO335_002948"
     CDS             3683..3922
                     /locus_tag="OO335_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003255.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="OO335_002949"
     CDS             complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="OO335_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6003256.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            5033..6406
                     /locus_tag="OO335_002950"
     CDS             5033..6406
                     /locus_tag="OO335_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6003257.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="OO335_002951"
     CDS             complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="OO335_002951"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6003258.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7525..9573)
                     /gene="prc"
                     /locus_tag="OO335_002952"
     CDS             complement(7525..9573)
                     /gene="prc"
                     /locus_tag="OO335_002952"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6003259.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="OO335_002953"
     CDS             complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="OO335_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6003260.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10388..10885)
                     /gene="msrC"
                     /locus_tag="OO335_002954"
     CDS             complement(10388..10885)
                     /gene="msrC"
                     /locus_tag="OO335_002954"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6003261.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            11015..12298
                     /gene="yebS"
                     /locus_tag="OO335_002955"
     CDS             11015..12298
                     /gene="yebS"
                     /locus_tag="OO335_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR6003262.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12267..14887
                     /gene="yebT"
                     /locus_tag="OO335_002956"
                     /pseudo
     CDS             12267..14887
                     /gene="yebT"
                     /locus_tag="OO335_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14967..16406
                     /gene="rsmF"
                     /locus_tag="OO335_002957"
                     /pseudo
     CDS             14967..16406
                     /gene="rsmF"
                     /locus_tag="OO335_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16524..16760
                     /locus_tag="OO335_002958"
     CDS             16524..16760
                     /locus_tag="OO335_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR6003263.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16865..17056
                     /locus_tag="OO335_002959"
     CDS             16865..17056
                     /locus_tag="OO335_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR6003264.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(17081..>17265)
                     /locus_tag="OO335_002960"
     CDS             complement(17081..>17265)
                     /locus_tag="OO335_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR6003265.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
BASE COUNT         4187 a         4354 c         4161 g         4563 t
ORIGIN      
        1 gtatcaactc aataacttat gaatttagcc aaagctatgt ttagtgtatt tttaataatc
       61 agacatagct taggctatat tatctcttcc cttatttgtt atttatttta acgtttcatt
      121 gatatataaa cataaatgaa aatcgttatc aataaagcaa tgaaataata tattccaaca
      181 gttgttttat attctcaaaa tatgttaagg ttgcgccctc atttggggag tagccgattt
      241 ccagattccg gaaatgtacg tgtcaacata ctcgttgcaa aacgtggcac gtacggactg
      301 aagactttca gtcaggcgag accatatgca catcaatcgc tatgcctgca tgaacgcgaa
      361 aacccatcgc ggcttgttgt tcatgtatgc atgtttgctg ggggcgatga tgtgttttat
      421 ggataccccg gtcaggacgt tgtcatgaat atcactgcta ctgttcttct tgcgtttggt
      481 atgtcgatgg atgcatttgc tgcatcaatc ggtaaaggtg cccccctcca taaaccgaaa
      541 ttttctgaag cattgcgaac cggccttatt tttggtgccg tcgaaaccct gacgccgctg
      601 atcggctggg gaatgggcat gttagccagc cggtttgtcc ttgaatggaa tcactggatt
      661 gcgtttgtgc tgctgatatt cctcggcggg cgaatgatta ttgagggttt tcgtggcgca
      721 gatgatgaag atgaagagcc gcgccgtcgg cacggtttct ggctactggt aaccaccgcg
      781 attgccacca gcctggatgc catggctgtg ggtgttggtc ttgctttcct tcaggtcaac
      841 attatcgcga ccgcattggc cattggttgt gcaaccttga ttatgtcgac attagggatg
      901 atggttggtc gctttatcgg ctcaattatt gggaaaaaag cggaaattct cggcgggctg
      961 gtgctgatcg gcatcggcgt ccagatcctc tggacgcact tccacggtta ataagaacgc
     1021 tgccagaggt gaatattaaa gtccgtctgg cagtcgaaca cttcttttgc tgccagtgtt
     1081 tgccagactt ctggcttcgc acgccaggca aacggcgtca tctgcaataa tgcgacggct
     1141 tcatcaccgc gaagacgcat cggataacac aactccgcac tctgctgtaa tgtaaaacct
     1201 tccagttgtt ctgcatgagg tgcatgaaga tgtacttcat tgtaaatcag ccccttcagc
     1261 tccatcaaat gtcgcggtcc cggcgtggca gtaatgaccc agccgccggg cttcactact
     1321 cgtgctaatt cttctgcttt acacggcgcg taaatacgta ttatggcgtc catactggta
     1381 tcggaaaacg gcaaacggtg gctggaagcg acacaaagag tgacctgcgg atagcgtttc
     1441 gccgccgctt ttatcgctac cttcgagaca tccaggccaa acgtggtgat ttcgggcaac
     1501 gcatcggcaa atgcgtgtgt gtaataccct tcaccacagc caatatccag caccgccgtg
     1561 gccttttcat caagccgttc ccgcagttgg gcgacaattg catcacgcag cggctgataa
     1621 tgtccggcat ctaagaatgc gcggcgtgct tgcatcattt ccgcgctgtc gcccggatca
     1681 cgagaccgtt tatgctgaac gggcagcaga ttgacatacc cttctttcgc catatcaaac
     1741 tgatgtcgtt ggggacagat atagctgttt ttttcacgcg aaagaggctg atggcaaagt
     1801 ggacaggaaa acgacatgac aactccggca ggatactaaa ggccgcaagt gtaacgcgaa
     1861 tcacgcggcg ggcaaatatc cgaacgccag gagcgaagat aaagcatagt aaaaagcctc
     1921 gcattcgacg aggctttata gcgagattga agcgtattca cacttcagat cagtggattc
     1981 gatcagatag ctgttacgtt aacagctgcc ggacctttct ggccgtcctg aatttcgaac
     2041 tcaacgttct gaccttcagc cagagttttg aagccattac cctggatagc ggagaagtgt
     2101 acgaacacat ctttgctgcc atcagccgga gtaatgaagc caaaaccttt agactcgttg
     2161 aaccacttaa cctgaccttt aatctttgcc atttgaaaaa ttccttagat tgttttcttc
     2221 gcccgcaggc ataacataga taaaactgac acattactgc atgaggcacc aatataaggc
     2281 tcggcagaga agcggtattc aacgtcaacg tgtttactca ggacttcttt actgaaaatg
     2341 ccacacataa acagaactgt acctcgttta acccgaaatc ttttatcaca tacaacgtta
     2401 attatggcaa gccattttta aacatatctc gatcagacgc acaaatctcg atacgtcttc
     2461 cacttttttt gcacacttat gcaacggaat acgcgccgat atatcattgt gcttaacctt
     2521 gccagttcag gcagatactt aaactggcgt agtttctaac atagttcaat aagttcagtt
     2581 gttccaggcc agagggagaa gttattacat agtgcgtgca atatcacatt ttctgctatg
     2641 caatgaataa aaagttatat cactttttct cataaaacag tcagttaacg gctatcaatt
     2701 accctaaaga gaagtcaatc cccaaaggga ttgtaaattt aaaataagaa aaattgatga
     2761 gtgagcaaaa aaaatcaaga gagaaacgtt tcgctaataa ataataccac gcttaatcac
     2821 aaatctgatg taaacatcct aataaattaa tgaggaaaat atctttttct gatatcccgt
     2881 tacgcaaata aataatatga taattatttg aagtcagacc agagttttaa tttgaagcag
     2941 ttgcaccatg acagtgcgta taatcaggat gaattgagta acccttcaac gacggggtta
     3001 ctcaagattg aggaaggatt atcgttcggc gaccaggcga ataatatctt cgtcatcaac
     3061 cgatttttga ctctcaatgg tctttttatc tgctttctct ggttcgtttg cttcgcacaa
     3121 acgacgtaac agtgcatttt gacgtttttg ctgatccagc aacgcctcaa gcagttcaat
     3181 ctgttcgtta gtccgtgaac tggcacgatt gataaaaaac cacaagatga gcccgataag
     3241 aagaaccacc accgatacaa ccaaagacgc aatgttcatc acgcctgaat ttacaacttc
     3301 gttcatttca ccacctctga gtagaggcgc tattctacca ctgctggaga ggaagaaaat
     3361 ctagtgctga aaaaatgata tcaccacggg ataaactggt taatggcaca aattccgctg
     3421 aaaaattgta catcctgatc gcacatcatg ttgaatacct gcgcccaaag cagcaagcaa
     3481 gccaacacca cgacaaccag aacgacccat cgaaactttt tcactccaat ctccgcttca
     3541 cctaccttat gtcattaaac tagcatgcgt tagttaaaca gcgcctaact atgtcgatat
     3601 tcgtgctttt tcggaatgag tagcttgctt cagcgcatat tgccgctacg attaagcgaa
     3661 cataaaaaga gaagagattg taatgcgatt catcattcgc acagttatgc tgatagcact
     3721 ggtatggata ggattattac tcagcggata tggcgttttg attggaagca aagagaatgc
     3781 cgcaggatta ggtttgcaat gtacctatct gactgccaga ggcaccagca cggtacaata
     3841 cttgcacact aaaagtggtt tcttggagat aacagattgc ccactgttac gtaaaagcaa
     3901 tatcgtggtc gataatggtt aagggtaata aataaccaga ttctcactca tgagtaaaat
     3961 attcttatag caagcgcgtt taatataacg caccttcaac tttatttttc tcatgctaaa
     4021 taaagaacct gtctgtttga tattccgaca ggttcttact ctcatcagaa cggatagtcg
     4081 tgataaccca tttgggcaga aattttgcgc gctgcggtat gcaacattgc gacatattcc
     4141 tgtaaacgct cttcagagaa acgcaacgtc gggaaggaga tgctcaaacc ggcaatgacc
     4201 acgccaaagc gatcaaatac cggtaccgca atgcattgca gcccttcttc ctgctcttcg
     4261 ttatcttctc catacccctg ctcgcgcacc tggtccagaa cgggtaataa cgcttctgta
     4321 ctggtgatgg tccgctcggt actgcgttta tactccacgc cctcaagaat ttgcttcact
     4381 tcatcgcgat cgcgccatgc cagcagtacc ttaccaatcg cggtgctgta cagcggatta
     4441 cgacgcccaa tccgtgaata catgcgcaaa ttgtacatag agtcaatttt gtgaatgtaa
     4501 acaatactgt cttcgtccag tgcgccgagg tggatagttt ctttggtcag gcgggagatc
     4561 tcacgcatct ggatatctgc gctacgaatt aaatcgacgt tttgtaacgc gcgagcgccc
     4621 agttcaaaca atttcagggt cagggaatat ttctccgact ccccttcctg cgccacataa
     4681 cctaaggttt tcatggtctg taaaaagcga taaacggtgc tttttgacat catgacgcgc
     4741 tgcgacagct cggttatccc tatttcgcgc tcttcaccca gcgcctgcaa aatgccaaaa
     4801 acttttagca cggaagatac agaatcaggc tgtttatcca gatctgcgtt agccatttat
     4861 cacctcattg cgagtgtttt ataaaaatca gaactgtttt ttattataat ttcgcaccag
     4921 ggtggtcgca atccatcttt tgccggttag ttacaattct gcgacatcca ccgtgaatat
     4981 cagtgctaga atcatacccc tgttgattat tcaccaaaga tataaaattc ctatgccaaa
     5041 agttcaggcc gacggcctgc cattgcccca gcgatacggt gcgatattaa ccattgtgat
     5101 tggtatttcg atggctgtcc ttgacggcgc aatcgccaac gtcgccctgc caacaattgc
     5161 cacggacctt catgccacac cagccagttc catctgggta gtgaacgcct atcaaatcgc
     5221 cattgtcatc tccctgctct cgttttcgtt tctgggcgat atgtttggct atcgacgtat
     5281 ttataaatgc ggtctggtcg tttttctgtt gtcttcactg ttctgcgccc tttctgattc
     5341 gctgcaaatg ctcacccttg cgcgtgtcat acaaggtttc ggcggtgcag cgttgatgag
     5401 cgttaatacc gcacttatcc gcctgatcta tccacaacgt tttctgggga gagggatggg
     5461 cataaactcg tttattgttg ccgtctcttc tgctgccggg ccgacaattg ctgcagcaat
     5521 cctctccatc gcatcctgga aatggttatt tttaatcaac gtaccgttag gtattatcgc
     5581 cctgcttctg gcgatgcgtt ttctgccacc caatggttct cgcgccagta aaccccgttt
     5641 cgacctgccc agcgccgtga tgaacgcgtt aaccttcggc ctgcttatca ctgcgttgag
     5701 tggtttcgct caggggcaat cgctgacgtt aattgctgcg gaactggtgg taatggttgt
     5761 tgttggtatt ttctttattc gccgccagct ttctcttccc gtaccgctgc taccggtgga
     5821 tttactgcgt atcccgctgt tttcactttc tatttgcaca tctgtttgct ctttctgcgc
     5881 acaaatgctg gcaatggttt ccctgccctt ttacctgcaa accgtgctcg ggcgtagtga
     5941 agtcgaaaca ggtttacttc tgacaccgtg gccgttagca acgatggtga tggctcctct
     6001 ggcaggctat ttgattgaac gcgtacatgc aggattgctg ggtgctttag ggttgttcat
     6061 catggctacg gggctttttt ccctggttct gctgcccgcg tcacctgcgg atatcaatat
     6121 tatctggccg atgatcttat gtggcgctgg atttggctta ttccagtcac ccaataacca
     6181 caccattatt acctccgctc cgcgcgaacg tagcggtggg gccagtggca tgttaggaac
     6241 ggctcgtcta ctgggtcaga gtagtggcgc ggcgctggtg gcgctgatgc taaatcagtt
     6301 tggtgataat ggtacgcacg tctcgctgat ggctgcggct attctggcag tgattgctgc
     6361 ctgtgtcagt ggtttacgta tcactcagcc acgagccagg gcataaaaaa agcgcgtcga
     6421 tcaggacgcg ctttttagta tttacttcat attacttcag gtattcaccc gtacgcagag
     6481 cttcaatacg tttatccagc ggcgggtgag tcatgaacaa ctcactgagc gatttcgact
     6541 taccgttaat gcagaaagcc atcatgctgg ttgcttcttg cggttcatag ctggttttca
     6601 ggcgctgcag cgcggcaatc attttctcgc gaccaaccag ttttgccgaa ccggcatctg
     6661 catggaattc acgatgacgc gagaaccaca tggtgataat gctcgccaga ataccaaaca
     6721 ccagttccag aaccgttgca accgcaaagt agatcagcgg gttgccgttg ctctcttcac
     6781 cttcatcacg atttccgccc ataaaacccg cggcaagctg cgccagaata cgggaaataa
     6841 agataacgaa ggtgttcacc acgccctgaa tcagcgtcat ggtgaccata tcaccattgg
     6901 cgatgtggct gatttcgtga gcaattaccg cctcggcttc atccgggctc atgttctgca
     6961 gcaaaccggt gctgacagca accagagagg catcacggcg cgcaccggtt gcaaaagcgt
     7021 tgatgtccgg cgcatggtag atagccactt gcggcatagc gatccccgcc tgacgagcct
     7081 gggttgctac agtattgacc agccaacgtt ccctttcgtt acgcggttgc tcgatcactt
     7141 ccccgccaac agatcgtaat gccatccatt tggacatcag aagcgaaacg aaggaaccac
     7201 caaaaccgaa cagcaaggcc atgatcatca gcccctgaac gctgctcgac tgtatccctg
     7261 tcaggctcag taccagcccg aaaacgacca ttacggccag gttcgttagc aggaagagcg
     7321 cgattcgcat cataattttc tttttacctc agtttaacaa aacgcaatat gcgataccca
     7381 catcgtatgg gttacgcgac tattttcaag tctggatagt gcgtaagtca ccagaaagac
     7441 acaactttac attttgtagc atctgattta cggcatcttg ccgctgttaa aaaatcaggc
     7501 acaatttctt gtgcctgatt gatattactt gacgggagcg ggttgttccg cgggtctggc
     7561 tttttcaagc ttcgccagat cgagtgcgat attcaccgtc tcatccagat aaggatccgg
     7621 ctcctggtaa tctttcgata gatcatccag cttcttcaac tccggtttac cttctcgttt
     7681 aaagcgttcg ttcaaacgcg ccagacgcgt cgcatcatct tcattattct ctttctcacg
     7741 cacagcgtag ttcagagaaa cgatattgcg cttgtccttc atagcgttga agcgcgcgat
     7801 atccttcatg atgttctgga actcaggatc tttcgcgata cgcgcattat gttccttcag
     7861 cagctccggt tcaaaggcag ttaaatctcc tgatttcaca taagtcgcgg catcgatgct
     7921 atcccacggc agcgcgttat cttcgaattt ctcacccgtt tccgtttctt cattacccgt
     7981 cggcatgatg atgtctggtg ttacgccttt acgttgcgta ctgccgccgt taacgcgata
     8041 gaatttctgg atcgtgtact gcacagaacc cagcgctggc cattcaggac gtaacatctg
     8101 atcgtaaata cggttcaatg aacggtattg ctgaacggtg cctttaccaa acgtcggttc
     8161 acccacaacc agcgcacgac cgtaatcctg cattgccgcg gcaaagattt ctgaagccga
     8221 agcactgaaa cggtcaacca gtaccaccag cgggccttta tagaacacct gcccgtcggt
     8281 atcgctgtct tcacgaacct tgccgttgtt atcgcggacc tgaacaatgg gacccgcagg
     8341 aataaacaga cctgagagcg ataccgcttc ggtcaacgcc ccaccgccat tgctacgcag
     8401 gtcgatgatc acactgctga cattctgttt ttccagtttc tgcagttgaa ctttgacatc
     8461 gtctgtcaaa cccacataga agcccggaat atccagcacg ccgactttct ctttaccgac
     8521 ggtcttcacc gacattttaa ccgcgcggtc ttcgagacga atacgttcac gggtcaacgt
     8581 tacagtacgg gtcttggtcc ctttaccagc aggtaaaatt tccagacgaa ctttactgcc
     8641 cttcggccct ttaattaagg caaccacatc atcaagacgc cagccaatca cgtcaaccat
     8701 cggcttgcct gtttgaccaa caccgacaat tttgtcacca acgctgatag ctttactctt
     8761 cgctgccgga ccacctgcca ccatcgaatt gataacggtg tagtcatcat ccatttgcag
     8821 cactgcgcca ataccttcca gtgacaaact catttcagtg ttgaactgtt cggtattacg
     8881 cggggaaaga tagttggtat gcggatcgat ttcacgcgca aacgccgtca ttgccagcga
     8941 gaaaacatct tcgctgttgg tttgcgccag acgacgaatg gcaaatttgt agcggcgtgt
     9001 cagggtttca cgaatttctt tatccgtttt tcctgtcagc ttcaggctta actcgtcgaa
     9061 tttgacttta ctgtcccact gcgcgttcaa ctcagcctcg tttttcggcc agggcgcttt
     9121 gctgcggtca aggttataag tgtcgttgcc ggtgaaatcc atcggctttt ccagtaccga
     9181 caaagcgtac tggtaacgtt caaaacggcg cttttgcgcc agattgtaga gatcgtagaa
     9241 aacgtcgagt ttgcctgaac gcagttcatc gcctaactcg gttttctttt tcgcgaactg
     9301 ttcaacatcg cttgccagca gcacattgtg gctgtaatcg agcagattca ggtagcggtc
     9361 aaagattttg gccgaaaatg cctgatcgag gtcgaactgg cgataatgag aacgggtgaa
     9421 gcgcgacgtt acgcgctcac ttaccgtcgc atgctgcgtc tcttccttta ataccggaat
     9481 ttgatcagca cgcgtgatat cttctacagc gaaggtctgg cctgctattg caagcaggcc
     9541 agctaacgcg gtaagcctaa aaaacatgtt catgcctggc ccggcctccg tttcagaaca
     9601 ccaggtgttc tgcgcgcaca atcaaagaca tacccgaatt cagctggacg cggacgccgt
     9661 ctttggtgat ttctaatacg gtggcatcca tcgcgttttg acccgctttc accttcaggg
     9721 cttgtccgac agtcagagct gaaatgtcag aaaccggggt gtgctgttct tcgcgaggtg
     9781 cttttactgt ttttggcgct ttctctaccg gcttttgcgc acgaggttta cgttcagcgc
     9841 cttctttgcg gcgtggcgtg gtcggacgtg gcttgcgttc gcggcgcggt gcgtcttctt
     9901 tctcaccagc agctgcggca gcttcgcgtt ttttcgcttg ctgttcagca cgctgtgcct
     9961 gaacacgcgc tttcgcttct tcaagctgct tgcgagcatg ctctacatgt tgctcgtcca
    10021 gctcaccgca tgggttgccg tcaagatcga cacgcgttgc gccgggttta acaccgtaaa
    10081 gataacgcca gctcgaagtg tagagacgta aagcggatcg taattgcgtt ttgctcaggt
    10141 tcatttcccc ggcaacacga tcgaccaaat cctgaaaaat accaattttc agcggacgcg
    10201 cttcaccttc cgcactgaaa cagtggggaa aacgttcggc cagaaacgcg attacttctt
    10261 tactgctatt caacttaggt tgattttcca tgaaatttcc tgattacaac ggacgtagcc
    10321 aacaagcgca ggcatgaaca ggcgtcatta taatgacgct accagtaaat gctacgttat
    10381 ccgttgatta tcctgcgacg ctcgcaaaga attttttgta atccgtcgtt gcaagcactt
    10441 tttcaagctg tgccacaagc tgacgtaagc cttgctcgtc ctcgtctgta aagcgagcga
    10501 agacagtact atcgatgtcg agaacaccaa taatctgatt tttcaccacc agcggcagaa
    10561 caatttcaga attactcgcc gcatcacagg caatatgccc gtcaaacgcg tgaacatcct
    10621 cgatacgctg cacttgattg cgggcaaccg ctgtgccgca caccccgcgc ccgacgggta
    10681 tccggacaca ggcaattttg ccctgaaatg gtccgagtac cagtgtatcg tcctcaagca
    10741 aataaaaacc tgcccagtta acgtcagtga gacgctcgta taacaacgca ctggtgttcg
    10801 caagcgttgc cagaaaactg gtttctcccg ccatcagcgc gttaaagtcg cgatttaaat
    10861 ccgcgtaaaa ttctgttttg ttcattactt aatcacttgg ttgtcttacc tggaactgcc
    10921 agcctattaa aataagcatt aaatgcgtta atgctcaaga tcattcccat catgggttaa
    10981 gattaatgtt aattcttatt acatttggca cgtcatggct cttaacacac cacaaattac
    11041 gccgacaaaa aagataacag tgagggcaat cggcgaggaa ctgccgcgtg gtgattacca
    11101 acgttgcccg caatgtgaca tgctgtttag cctgcccgag ataaattctc atcaaagtgc
    11161 ctattgtccg cgctgtcagg caaaaattcg cgacgggcgc gactggtcgc taacgcgcct
    11221 ggcggcaatg gccttcacca tgctgttgct tatgccgttt gcctggggcg aaccgctgtt
    11281 gcatatctgg ctgttaggca ttcgtattga cgccaacgtc atgcaaggca tctggcaaat
    11341 gaccaaacag ggcgatgcga taacgggatc aatggtcttt ttctgcgtta ttggtgcccc
    11401 cctcattctg gtgtcctcca tagcatattt atggtttggt aaccgactgg gaatgaattt
    11461 acgtccggta ctgctgatgc ttgagcgact taaagagtgg gtaatgctgg atatctacct
    11521 ggtcggcatt ggcgttgctt ctataaaggt acaggattat gcccatatcc aggcgggtgt
    11581 cggcttgttc tcttttgtgg cgttggtgat tttaacgacg gtgacgttgt cacatcttaa
    11641 tgttgaggag ctgtgggagc gatattatcc gcagcgcccc gctacgcgta gggacgagaa
    11701 acttcgtgtc tgtcttgggt gccattttac cggctatcct gatcagcgtg gtctctgccc
    11761 gcgttgccat atcccgctac gcctgcgtcg tcgtcatagt ttgcaaaaat gctgggcggc
    11821 gctgttagcg tcaatcgttt tgttgttacc agccaaccta ttgcctattt ctatcattta
    11881 tctgaatggt ggccggcagg aagatacgat tctttccgga attatgtcgc tggcaagtag
    11941 caacattgcg gttgcgggaa tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt
    12001 gatcgtcatg ttcactttac tgttgagcat tcattttaaa tgccagcaag gtttacgcac
    12061 acgcattctg ttactgcgga tggtgacctg gattggacgc tggtcgatac tcgacctgtt
    12121 tgtcatatct ttaaccatgt cgctgattaa tcgcgatcag atcctcgctt ttactatggg
    12181 accggctgcg ttttattttg gcgctgccgt aattttgact attcttgctg tggaatggct
    12241 ggacagccgc ttactttggg atgcacatga gtcaggaaac gcccgcttcg acgactgaag
    12301 cgcagattaa aaataaacgc cgtatctcac ctttctggct gctgcctttc atcgcgctaa
    12361 tgattgccgg ttggctgatt tgggacagtt atcaggaccg gggtaatacc gtcaccatcg
    12421 actttatgtc ggcggatggt attgttccgg gccgtacgcc tgttcgttat cagggcgttg
    12481 aagtcggaac agtgcaggat atcagcctca gcgacgatct tcgtaagatt gaagtcaagg
    12541 tcagcatcaa gtccgatatg aaagatgcgc tgcgcgaaga gactcagttc tggctgatga
    12601 cgccaaaagc atcgttggca ggtgtctccg ggctggacgc cctcgtcggt gggaactata
    12661 tcggcatgat gccgggtaaa ggtaaagagc aggatcactt tgtcgcgctc gatacccaac
    12721 cgaaatatcg gctggacaat ggcgatctga tgatccacct gcaagccccc gctctcggtt
    12781 cgctgaacag cggttcattg gtctatttcc gcaagatccc ggtgggaaaa gtctacgact
    12841 atgccatcaa tcccaacaag caaggcgtgg tgattgatgt cctgatcgag cggcgtttta
    12901 ccgatctggt gaaaaaaggt agccgtttct gaaacgtttc cggcgttgat gccaacgtca
    12961 gtatcagtgg cgcgaaggtg aaactggaaa gtctggcggc actggttaac ggtgcgattg
    13021 ccttcgattc acctgaagag tcgaaacctg ccgaggcgga agataccttt ggtctgtatg
    13081 aagatctggc ccacagccag cgtggagtaa taataaaact ggaactgccg agtggagcag
    13141 gattaaccgc cgactcgacg ccgttaatgt atcaggggct ggaagtcgga cagctgacta
    13201 aactggattt aaatcctggt ggtaaagtca ctggggaaat gaccgttgat ccctgcttcg
    13261 tgaaaatacc cgcatcgaat tacgcaaccc gaaattatcc cttagcgatg ctaatctcag
    13321 cgccctgctg accggaaaaa cctttgagtt ggtgcccggc gatggcgagc cacgcaaaga
    13381 gttcgttgtt gtgccgggcg aaaaagccct gctgcaagag ccggacgttc tgacactgac
    13441 cctgacagca ccggaaagtt acggtattga tgcgggtcag ccgctcattc ttcacggcgt
    13501 gcaggtaggc caggttatcg atcgtaaact caccagcaaa ggcgtcacct ttaccgtcgc
    13561 catcgagcct cagcatcgag aactggtaaa aggcgatagc aaatttgtcg tcaacagccg
    13621 tgtcgacgtg aaggtggggc tggatggcgt tgagtttctc ggtgccagcg cctcagaatg
    13681 gattaacggc gggatacgta ttctgccggg cgataaaggc gagatgaaag ccagctatcc
    13741 actgtatgcc aatctggaaa aagcgctgga gaacagcctt agcgatttac ccaccacaac
    13801 catgagtttg agtgcagaga cgctgccgga tgtgcaggca ggatcggtag tgctgtaccg
    13861 taaatttgaa gttggtgaag ttattaccgt gcgtccgcga gctaacgcgt tcgatatcga
    13921 tctgcacatt aaaccggaat atcgcaacct tttgaccagc aacagcgtgt tctgggcaga
    13981 aggcggggcg aaagttcagc tgaatggtag tggcctgacc gtacaggcat ccccgctctc
    14041 cagagcatta aagggggcca ttagcttcga taacctcagt ggtgccagcg ccagtcagcg
    14101 taaaggcgac aagcgaattc tgtatgcttc cgaaacagcg gcccgtgcgg ttggcaggca
    14161 gattacgctt cacgctttcg atgccggaaa actggcggtc gggatgccaa ttcgctatct
    14221 cggtattgat atcgggcaaa tccagacgct ggatctgatt accgcacgca atgaagtaca
    14281 ggcaaaggcg gtgctctatc cggaatatgt ccagaccttt gctcgcggtg gtacgcgctt
    14341 ctcagtggtc acaccgcaaa tctcggcagc tggcgttgag catctagata ctatcctcca
    14401 gccgtatatc aacgtcgaac caggccgtgg caatcctcgt cgcgactttg aattagaaga
    14461 ggccaccatt actgattcgc gttacctaga tggtttaagc attattgttg aagcgccgga
    14521 agccggttcg ttaggcatcg gtacgcctgt gctgttccgt ggtctggaag taggtacggt
    14581 taccggaatg acgctgggga cattgtcaga tcgcgtgatg attgcgatgc gcatcagtaa
    14641 acgctatcaa cacctggtgc gtaacaattc cgtcttctgg ttggcatcgg gttacagtct
    14701 ggactttggt ctgacgggcg gcgtagtgaa aaccggcacc tttaaccaat ttatccgtgg
    14761 cggcatcgcc ttcgccacgc ctccgggtac gccacttgca ccgaaagccc aagaaggcaa
    14821 acacttcctg ttgcaggaaa gtgaaccgaa agagtggcgt gaatggggaa ctgcgcttcc
    14881 caaataatgc ccactgctcc ggcgtgcctg cgccggagcg cttatgctaa actgcgcgcc
    14941 tgtttttttg ccagtggtac atgctcgtgg cccaacacac cgtttatttc ccggacgcgt
    15001 ttctgacaca aatgcgcgaa gctatgcctt cgacgctctc atttgatgat tttcttgccg
    15061 cctgtcagcg cccgttgcgc cgcagcattc gcgttaatac gctgaaaatc tccgttgctg
    15121 atttcctgca attaaccgct ccttatggct ggacgcttac gccaattccg tggtgtgaag
    15181 aaggtttctg gattgaacgc gacaatgaag atgcattgcc attgggtagt accaccgagc
    15241 atttaagtgg cctgttttat attcaggaag ccagttcaat gttgcccgtc gccgccttgt
    15301 ttgctgacgg taatgcacca cagcgggtga tggatgtcgc tgctgcgcca ggctcaaaaa
    15361 cgacgcaaat tgccgcgcgg atgaataacg aaggggcaat ccttgccaat gagttttccg
    15421 ccagttgggt aaaagtgtta catgccaata tcagccgctg tggcatcagt aatgttgcgc
    15481 tcacacattt tggtggccgc gtgtttggtg cggcagtgcc agaaatgttt gatgccattt
    15541 tgctggacgc tccctgctcc ggcgaaggcg tggtgcgtaa agatcccgat gcgctaaaaa
    15601 actggtcacc cgaaagcaat caggaaatcg cagctaccca acgggaactg attgacagcg
    15661 cctttcatgc attacgtcct ggcggtacgc tggtttactc gacctatacc ttaaaccggg
    15721 aagaaaacga agccgtttgc cggtggctga aagagactta ccccgacgaa gtagagtttt
    15781 taccacttgg cgatctcttc cctggtgcaa acaaggcgct gaccgaagaa ggttttttgc
    15841 atgttttccc acaaatttac gactgcgaag gcttcttcgt tgctcgtctg cgtaaaactc
    15901 aggcgatccc cgccttaccc gccccaaaat acaaagtggg caatttcccg ttcagcccgg
    15961 tgaaagatcg cgaagccgga caaattcgtc aggcggctgc aagtgttggc ttaaactggg
    16021 atggaaacct gcgactctgg caacgcgaca aagaactgtg gttgttcccg gtaggcattg
    16081 aagccctgat cggtaaagtc cgattttctc gcttagggat taaacttgcc gaaacgcata
    16141 acaaaggtta tcgctggcag catgaagcgg ttattgccca tgcctccccc gacaatgtaa
    16201 acgcttttga actgacaccg caggaagcgt aggagtggta tcgcgggcgc gatgtttacc
    16261 cgcaagccgc gccagtggcg gatgacgtgt tggttacttt ccagcatcag ccgattggtt
    16321 tagccaaacg gattggttcg cgactgaaaa acagctaccc gcgtgaactg gtgcgggacg
    16381 ggaaactttt taccagtaac gcatgacagc gcacaaaaaa agcgcacttt ttgactggca
    16441 cattcgccgg cctcaactag gctgaaaaat tgtgcgatcg gactggtcgt actacaatca
    16501 gcagctaaat ggagagcacg aagatgaaaa ccagtgtgcg cataggcgct tttgaaatcg
    16561 acgacggcga attacacggt gaatcgccgg gtgatcgaac gttaaccatt ccttgtaaat
    16621 ctgaccccga tttatgtatg caactggatg cctgggatgc tgaaaccagt atccctgccc
    16681 tgctaaatgg cgaacactct gtcctttacc gtacccgtta cgatcaacaa tctgatgcct
    16741 ggattatgcg tcttgcctga tccaaaaaga acccgtcggc atggcgggtt atttgtcctg
    16801 gttattcccc cgttgtaaaa tctctcctaa acttaacggt acggcaccac acttcgggga
    16861 tgaaatgttc gcgctggtac tttttgtttg ctacctggat ggcggttgtg aagatattgt
    16921 tgtggatgtc tacaacacgg aacagcagtg tctttattct atgagcgatc aacggatccg
    16981 ccatggcagt tgttttccga ttgaggattt tatagatggt ttctggcaac cagcacagga
    17041 gtatggtgat ttttaattat tgcagttgca caagagtcag ttagccccca aagacagcac
    17101 cggtatcaat ataatgcagg ttgccaatat ccacgcgatg tcgcaacggt gtatgaccaa
    17161 accagaaatg atcagcacct gtaattccct gcccttttgg gcgttcacct aatcgcgagc
    17221 ggctccacaa gacctgatgc aaatcaacgt ccttttgcca ttcat
//
LOCUS       DAJTSF010000084        17090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000084 DAJTSF010000000
VERSION     DAJTSF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.084"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..508
                     /locus_tag="OO335_002961"
     CDS             <1..508
                     /locus_tag="OO335_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR6003266.1"
                     /translation="FREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGF
                     TQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIR
                     HNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQ
                     YSRAWTVK"
     gene            complement(509..1669)
                     /gene="ybdL"
                     /locus_tag="OO335_002962"
     CDS             complement(509..1669)
                     /gene="ybdL"
                     /locus_tag="OO335_002962"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR6003267.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1778..2866
                     /gene="hcxA"
                     /locus_tag="OO335_002963"
     CDS             1778..2866
                     /gene="hcxA"
                     /locus_tag="OO335_002963"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR6003268.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2876..3073)
                     /locus_tag="OO335_002964"
     CDS             complement(2876..3073)
                     /locus_tag="OO335_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6003269.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3255..5360)
                     /gene="cstA"
                     /locus_tag="OO335_002965"
     CDS             complement(3255..5360)
                     /gene="cstA"
                     /locus_tag="OO335_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR6003270.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5541..5954)
                     /gene="entH"
                     /locus_tag="OO335_002966"
     CDS             complement(5541..5954)
                     /gene="entH"
                     /locus_tag="OO335_002966"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR6003271.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5957..6703)
                     /gene="entA"
                     /locus_tag="OO335_002967"
     CDS             complement(5957..6703)
                     /gene="entA"
                     /locus_tag="OO335_002967"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR6003272.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6703..7560)
                     /gene="entB"
                     /locus_tag="OO335_002968"
     CDS             complement(6703..7560)
                     /gene="entB"
                     /locus_tag="OO335_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR6003273.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7574..9184)
                     /gene="entE"
                     /locus_tag="OO335_002969"
     CDS             complement(7574..9184)
                     /gene="entE"
                     /locus_tag="OO335_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR6003274.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAETGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9194..10369)
                     /gene="entC"
                     /locus_tag="OO335_002970"
                     /pseudo
     CDS             complement(9194..10369)
                     /gene="entC"
                     /locus_tag="OO335_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10661..11617
                     /gene="fepB"
                     /locus_tag="OO335_002971"
     CDS             10661..11617
                     /gene="fepB"
                     /locus_tag="OO335_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6003275.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11621..12853)
                     /gene="entS"
                     /locus_tag="OO335_002972"
     CDS             complement(11621..12853)
                     /gene="entS"
                     /locus_tag="OO335_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR6003276.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12964..13968
                     /gene="fepD"
                     /locus_tag="OO335_002973"
     CDS             12964..13968
                     /gene="fepD"
                     /locus_tag="OO335_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR6003277.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13965..14957
                     /gene="fepG"
                     /locus_tag="OO335_002974"
     CDS             13965..14957
                     /gene="fepG"
                     /locus_tag="OO335_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR6003278.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14954..15769
                     /gene="fepC"
                     /locus_tag="OO335_002975"
     CDS             14954..15769
                     /gene="fepC"
                     /locus_tag="OO335_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6003279.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15766..16899)
                     /gene="fepE"
                     /locus_tag="OO335_002976"
                     /pseudo
     CDS             complement(15766..16899)
                     /gene="fepE"
                     /locus_tag="OO335_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3993 a         4863 c         4443 g         3791 t
ORIGIN      
        1 ctttcgtgaa aagccagtgg attgcgttct gtgggttaaa aacagccaac ttatgcctaa
       61 tgattacaac ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgcaaa aatccattga
      121 aatagatggt tttacccaac ccattgtggt cacacatacg gataaaaatg ccatggaaat
      181 cgtggatggt tttcaccgcc atgaaattgg taaaggctca tcatcattaa aattacgctt
      241 aaaaggttat ttgccggtga cctgtctgga aggtacgcgt aatcaacgca tcgccgcgac
      301 tattcgtcat aatcgcgctc gtggacgcca tcaaatcacc gccatgtcgg aaatcgtccg
      361 tgaacttagc cagttgggat gggatgataa taaaattggc aaagagctgg gaatggacag
      421 cgacgaagtg ctgcgactga agcaaattaa cggcctgcaa gagttgtttg ccgaccgtca
      481 atattcccgc gcctggacgg ttaaatagct aaagctggcg caggcgttca gctgctgcca
      541 gcaacgtcga ttccttcttg gcaaaacaga gacgaatcag tttatgtggg aagggatcgg
      601 cgcaaaacac cgacagcgga atcgccgcta cgccgtgctc ctgcgtcagc cactggcaaa
      661 actcaacatc atccagggta gaaaccgcgc tgtaatccac cagcaaaaag tatgtacctt
      721 cacacggtaa aatctccagc cggctttcat ttaaagcatt caccagaata tcgcgcttct
      781 ggcgataaaa gtccggtaac gcaagataat gctcaggttc tgcacgtagc atatcagcaa
      841 gcgccagctg tgccggggta ttcaccgaaa aggtcagata ctgatgtacc ttgcgaattt
      901 cggcgctgat gggcgctggc gcaacacaat aacccacttt ccagccagtc atatgatagg
      961 tcttgccaaa tgaagaaacc gccactgccc gctcacgcag ctgcggatgc gccagcacac
     1021 tggcatggcc ctgttgtgaa aagttgatgt gctcgtagac ttcatcgcta atgacaaaaa
     1081 tctcgtgccc ggcgatcgcc tgccacaaag cggcgaaatc agcctgctgc cagacagttg
     1141 cactggggtt atgcggagtg ttgaggatca ccagtctggt gcgctcgctt aataatgcgg
     1201 caaattcctg ccagtcaacg cgaaaatgcg gtggttgcag tgccatacgt ttcactattc
     1261 ccccagaaag cgcgatggcg ggggcgtaac tgtcatagct gggatcaaaa caaatcactt
     1321 catcgccatt gcgcaccagt gcggtaatcg ccgcgtataa cgcttccgtc gcccctgccg
     1381 ttacggtgat atcgctatcg gcatctggtt gatagccata caaacgttcc gttttctgag
     1441 caatcgcctc gcgcaaggcc tgcacgccgg tcatgggcgc gtattggttt gccccctggg
     1501 caacgtggta cgccagccgc tcctgtaaat agcgcggacc atcaaaatca ggaaagcctt
     1561 gcgacaggtt aatcgcctgg tgttgctgcg ccagcgcgct catctgggtg aaaatagtgg
     1621 tgccaagttg tggaagtttg ctttgtggaa tcagagggtt atttgtcatt tttattgtac
     1681 ctgacgaagt tgcgtgttga aggcactata acacgatgcc agtatttggc aatcaagacg
     1741 tttagatgtc taaataaaac aataaggaca acacaacatg cctcacaatc ctatccgcgt
     1801 ggtcgtcggc ccggctaact acttttcaca tccaggaagt ttcaatcacc tgcacaattt
     1861 tttcactgat gaacaacttt ctcgcgcggt gtggatctac ggcaaacgcg ccattgctgc
     1921 ggcgcaaacc aaacttccgc cagcgtttgg acttccaggg gcaaagcata ttttgtttcg
     1981 tggtcattgc agcgaaagcg atgtacagca actggcggct gagtccggtg acgatcgcag
     2041 cgtggtgatt ggcgtcggcg gcggcgcact gctcgacacc gcgaaagccc tcgcccgccg
     2101 tctcggtctg ccatttgttg ccgctccgac gatcgccgcc acctgcgcag cctggacacc
     2161 gctctccgtc tggtataacg atgccggaca ggcgctgcat tatgagattt tcgacgacgc
     2221 caattttatg gtgctggtgg aaccggagat tatcctcaat gcaccgcaac aatatctgct
     2281 ggcggggatc ggtgacacgc tggcgaaatg gtatgaagcg gtggtgctgg ctccgcaacc
     2341 agaaacgttg ccgctaaccg tgcggctggg gatcaataat gcgcaggcca ttcgcgacgt
     2401 cttgttaaac agtagcgaac aggcacttgc cgatcagcaa aatcagcagt taacgcaatc
     2461 attttgcgat gtggtggatg ctattattgc tggtggtggg atggtcggtg gtctgggcga
     2521 tcgttttacg cgtgtggcgg cggctcatgc cgtgcataac ggtctgactg tcctgccgca
     2581 aaccgagaag tttcttcacg gcaccaaagt cgcttacgga attctggtgc aaagcgcctt
     2641 gctgggtcag gatgatgtgc tggcgcaatt aaccggggcg tatcagcgtt ttcatctgcc
     2701 gactacgctg gcggagctgg aggtggatat caataatcag gcggagatcg acaaagtgat
     2761 tgcccacacc ttgcgcccgg tggagtccat tcattacctg ccagtcacgc tgacaccaga
     2821 tacgttgcgt gcagcgttcg aaaaagtgga atcgtttaaa gcctgacgtg caaatttagc
     2881 agcagcgcgc gccgcctttt ccaccgtagc gcgcgtcctg ccgctcacgg aaaaactcct
     2941 cgtaggtcat cggcgtttga tcgggatgat taacccgcat atgttcgaca tagttgtcgt
     3001 aatcaggcat accaatcatc agcttcgccg cctgacctaa atattttccg gcttttgcca
     3061 gtgaatcaaa catcttggcc tccgctaacc atgttgctga aagagtgccg gatgcggcgt
     3121 gaacgcttta tccggcctac aaatgctatc aacgcactcg gtcagtcccc tcgccccatc
     3181 ggggagaggg ttagggtgag ggggaaccct cggcactatg ccaacattcg ccaacatccc
     3241 ccctcactct gactttagtg cgcgcctttc gcctgcgcta cgatctcctc gacattttcc
     3301 ggcatcggct catacggcgt ttctttcgcc gttggcttcg gatctttcaa tgccgcaagt
     3361 gccgtcttaa tcgagaacag tgccaggacc accacgacca ccataaagaa gatggttaac
     3421 ccggcatcca gacggttgtt gaacaccagt tgcgccagct gtgactcagt atactgcgac
     3481 ggaatattgc cgctgtcgat cattgcctgg aacttgttag caatgaccag gaagccgact
     3541 ttcgcatccg ggctaaacgc tttcagccag tcagcggtca gggtacaaat cagcagccag
     3601 gccgttggta ccagcgccac ccaggcgtaa cgttgacgtt tcatcttgaa caacaccacg
     3661 gcacagagca tcagcgccat ccctgccagc atctggttgg caataccaaa cagcggccac
     3721 agagtgttaa taccgcccag cggatcgacc acgctctgat ggaggaagta gccccacgcc
     3781 agtacgcaca gcgctgttgc cagcaggtta gcaggcagtg aatcggtccg cttcaggcca
     3841 ggagacacca cgcccagcag atcctgcaac ataaagcgcg cagcacgcgt acccgcatct
     3901 accgccgtca gaataaacag agcttcaaac agaatggcga agtgatacca gaacgccaca
     3961 tccatcatac cacccagcgc accgtgcagg atgtaagcca tccccaccgc caacgtcggc
     4021 gcaccgcccg cacgggaaat gatcgactgt tcacccactt cgctggcaat ctggttgagc
     4081 gtatctggtg taatgctaaa gccccagcta ctcaccacct gcgcggcaga agcgactaca
     4141 tccgccgtcc ctgccggagc cagcaccgcc attgggctgt tcatggcaaa atacacgccc
     4201 ggatcgatga tacaggcaga aactagcgcc ataatcgcca cgaaggattc catcaacatc
     4261 ccaccgtagc cgataaagca cgcctgccct tcgttcgcca gcatcttcgg cgtggtgccg
     4321 gaagagatca gcgcatggaa gccagacacc gcaccacagg cgatggtgat aaacaggaac
     4381 gggaacaggt taccggtcca taccgggcca gtgccatcga caaatttggt cagcgcaggc
     4441 atggtcagcg tcgggcgcat aatcaaaatg cctaccgcca gaccgacgat agtcccgatt
     4501 ttcaggaagg tcgagagata gtcacgcggg gccagcagca accacaccgg cagcaccgcc
     4561 gccacaaaac cgtagcccac cagcatccag gtcagctgca cgccggtaaa gtcaaagtac
     4621 ggtgcccagg tcggactttc tgccacccag ccgccagaga taatggcgaa aatcaggaac
     4681 accaggccaa tcactgacac ttcgccaatg cggcccggac gcagatagcg caggtagatt
     4741 cccataaaaa tcgccagcgg aatggtgaac gcaacggtat acgttcccca cgggctatga
     4801 gtcagggctt tcaccacgat catcgccagt actgcaagga taatgaccat gatcataaag
     4861 caggctacca gcgcaatcac cccggcagtt ggccccatct cttctttgac cagctcaccc
     4921 agcgagcgac cgtcacggcg cgtagaaaca aacagcacca tgaaatcctg caccgcaccg
     4981 gcgagaacca ccccggcgag cagccagatc atccccggca ggtagcccat ttgcgctgcc
     5041 agcaccggcc ccaccagcgg ccctgctccg gcaatggccg caaaatggtg accgaacagc
     5101 actttcttgt ccgtcggcac atagtccagc ccgtcgttat ggcgcaccgc tggcgtcata
     5161 cgcgtcgggt caaccgccag cacatttttg gcgatataca gcccataaaa acggtaagcg
     5221 atcagataga tacagaccga cgccaccaca atccacagcg cgttgatctg ttccccacga
     5281 tttaaagcaa tgtatcccag agcaaatgct cccattacag agagcactgt ccagacgagg
     5341 tatttccctg atttgttcat agttgttatc cgtgtgcgtg tccatagaga tgttacattt
     5401 tgtttctata acatctttgt acgatttaac aacacggtaa ccatgtaaat cggagttgaa
     5461 accaatattt aacttaacaa tgttaactcg atcactccgt taaccgagtt ttcctgcaat
     5521 ctcatttaat tctgtcgggc tcatcccaaa actgccgtac ccagccgaca agtgcagcaa
     5581 cgccgcccct gttcatcgaa aacgacgatt tcccaactct gattttgcct cccaagatgc
     5641 agcggctggc agacgccgcg taccttgcct tcagacaccg ggcgatgatg ggtggcgtta
     5701 agttccgtgc ccaccacaca ctgcccgtca cgggtcatca taaatccggc catcgatccc
     5761 agcgtttcag ccagcgccgc cgacgcgccg ccatgcagca ggccaaacgg ctgatgagta
     5821 cgggtatcaa ccggcatttc ggcttccagc acatcatctc ccagaagggt atacacaatt
     5881 cccagatgcg ccaccattgt gttatcgctg gtggcgttca gttcgtcgag cgttaaatgg
     5941 cgtttccaga tcatgcttat gcccccagcg ttgagccgcc atcgaccaca atatcctgta
     6001 gggtgatatg gctggcgagg tcagaggcga ggaacaaaat cgtgttggcg atctcttgtg
     6061 gacgggcgat tttccccagc ggaatgccga gtttaaactg ctcgccaaag ccgcgaatac
     6121 gctgttcttc ggcgtcatcg ctcacccaca gcgtgcgttg catatcggtg tcggtggagc
     6181 caggcgaaac cacattacag cgcacgccgc tacccgccag ttccagcccg acgctcaacg
     6241 ccaggctttt cagcgccgct ttcgatgcgc cataagcact cataccaata cgcgccgtgt
     6301 gtgcggcgtc ggacgccaca gtgacaatcg ccccgccccg ctgacggcga aactggttca
     6361 tggtttgctg gaacaggtta aacgcaccgc cgacgttaac cgcaaaagtc tgctgccagt
     6421 cctctttact gagctgatcg gtcgcgccca tgcgcaaaat tcccgccgca ttgatcagca
     6481 cgtccagtcg ctccgtttca gctaacagtc gctgacatac ctgcgccacc tgcccggcgt
     6541 cggcaacatc cattacttcg gtcgcaaagg gatattgctc ctgagtgaac gcttgatcaa
     6601 aacctgtaac tttcgctccc gcctcaacaa acgtcagcgc cgtggcgtag ccgatacctt
     6661 tacctgcgcc ggttacccag acatttttac cgctgaaatc cattatttca cctcgcggga
     6721 gagtagcttc caccaggcgt cgatggtcgg gtttttcgcc agcatgacaa agtcgatatc
     6781 accatgcact ttgcgccagc gcgccgccag cgccatcatg cgcaccgaat ccagaccgta
     6841 gtcgatcagg ttgtcgtcat cgaacggttc atcggactcg tccagcaacg gcaggatcac
     6901 ctcacgcagc gccgctttgc tggcggggat aggtgctggc agtaattctt cggtcatcac
     6961 cacccggcca gaacgtccgg ccacatattt cagcgacatc aaatgctcgt cacggctgaa
     7021 atcagccagc gcatccgcca ccataaacgg tttaatatcg cgcataaatg cgtcggttgc
     7081 ggtggtcata cagccaatgt gggcatatac tcctgtaata atcagctggt tacgtccact
     7141 ctctttgagc atttgctcca gcggagaacg atgaaacgcg ctgtagcgcc acttcaccag
     7201 caccgtgtcg tcggcatctg gcgtcaggcg atccaccacc ttttgctgtt ccggcgagcg
     7261 ggtcaggccc ggcccccaca tatcattcaa cagcgcccga tcttcatcgc tctgctcttt
     7321 cggctgggcg gtgtaataaa ccgggatatt gtgctgtttg cagtagtcgc gcagcgcagc
     7381 aatattcgcg atcacctgct ccatcatcgg gcagttctcg ccccagaagc tgacaaaata
     7441 gtcctgcata tcatggatta acaacgcggc acgttgcggt tcaaaggccc agtcaacttt
     7501 attctgcgga atatcgtgag actccggcag tgcgtaagcc tgtaattttg gaatagccat
     7561 cgtgttctct ccttcaggct gatgcgcgtg acgccagcca ctgacgtaat tgttttttat
     7621 cgactttccc gaccgccgtc agcggaagtg aatccacaca ctccacgcga tccggtaatt
     7681 taaattcggc aataccctgt tcacgcagga aacgacgcac ctgcaccgcg cgcagcggct
     7741 cttttaccac cagataagca cagctttttt cgcccatcag ctcattttcc atgctcacca
     7801 gtgcggcata gatcactgcc ggatggctca gcagcaggtt ttcgatatct tcggcagcga
     7861 tcttctcgcc gccacggttg atctgatctt tctcgcgccc ctgcacggtg atgtaaccct
     7921 ctggatcaat agagatcaga tcgccggaac agtaaaaacc gttggcatca aaggcactgg
     7981 cattgtgctg cggacttttg taatagccac ggaaggtgta cggcccgcgc gtcatcaggc
     8041 gtccaacttc cccttgcggc agtggatttc cttcggcatc ggcaacccac acttcatcgt
     8101 ccggacacat tgggtaaccc tgggtatgga taattttctc cgcgctatca tcaagacggg
     8161 tgtagttcac cagcccttcc gccatgccaa acacctgctg caactggcag ccagtctcag
     8221 cgggaatacg cgctgcaagc gtggcagaaa gacgtgcgcc gccgacctgt aacagtttca
     8281 gcgaggcaag ctgcgcccgg ctttcgcctt cagccagcgc ctgcaaccac aggctgactg
     8341 ccggcggcac cagcgcggta acgttaacct gatgttttgc aatcaacggg aagcaaagcg
     8401 tggcgctggg atcggccgcc agaacaaccg ttcctccggc aagaaagacg cccagcgatc
     8461 ctggcgaact catagtgtag ttatgagccg ccgggatcga gcacaggtag cgcgtctgtt
     8521 gtgtgaactg acaaatctcg gcgctacgac gcacgctgta gtaatagtcg ttatgagtgc
     8581 gcgggatcag tttcggtgtg ccggtggtgc cgccggaaag ctggaaatag gccacttcat
     8641 cagcaggtga tggcgtggca gtaaaaccct ctgccggatg gttaatcgca tcctgcaagt
     8701 tatgctcacc gctgtcgttg tgcagttgca ccacgcgaat ggaagaatgc tctgcaacaa
     8761 atgtgttgag gaaatcatcc ccgctaaata gcgcatgctg gcgatcggca atcagtaacg
     8821 ccggttcaat ctggctggcg taggcgttca gttcactacg ctgatggctg aacagcgcca
     8881 gcaccggcgc gacgcccagt ttcagcagcg cgaaaaaggt aatgtaaagt tcagcgacgt
     8941 tacccagttg taccagcgcg gtttcaccag gtttaatgcc ctgacggcgc aaactacagg
     9001 cgaggttatc cgccgcctga ttcagctccc gataactcaa ctgccgctcg ccgtcgataa
     9061 ccgcgatgct gtcactcgca gcgtggcgag tcagaatgtc ggtcagtggc aaatcctgcc
     9121 agtagccttt ttcccgatag cgacgggcaa actcttccgg ccagcgggtg aatggaatgc
     9181 tcatcctcgc tccttaatgc aatccaaaaa cgttcaacat ggtagaaagt ttgacgcctg
     9241 tttcgcgcca ctcacccaac ggtgacgacg caggcacaat ccccgctccg gcaaacagac
     9301 gcacctgatt ttcccgcagc ttcgcgcagc ggatggtcac cacccattcg ccgttacctt
     9361 cgctgtcaca ccaacccaca atgccgccaa acagttcgcg gtcgaacggc tccagttcag
     9421 caataacctg ggtcgcggcc tgatgcggga aaccgctcag cgcgggggtc ggatgcagca
     9481 gacaggccag agtcagtgcg ttttcttgcg aattcgcttt accttcaaag ggagttgcga
     9541 gatgccacag cgtcggcgtg gtaatcaatt gtggagagga gggaacgtgt aactcactac
     9601 tgcgttcgcg cagtacctct ttcatcgcct gagtccccag ttcatgttca tggcgatctt
     9661 tttctgacgc cagcagacga ttacccgctt cgcgatcgag cacttcatcc ggctgacgac
     9721 gcgcggaacc ggctaacgga atggagctaa aacgctcgcc gtctttacgt agcagcagtt
     9781 ccgggctggc ccccagcagg acgccaccat cagccagcgg gacatggaag ttgtaactaa
     9841 ccgggttttg cgcaatcaac cgttccagca atacgccact atcaatggcg gcgtcagtgg
     9901 tgatatcaat caatcgtgac aacaccactt tgtcgacctg cggcgtggcg gtaagtgcgg
     9961 cggcgcgggc acccatctgt tcaaacgtgg tttgttcagg aattgcctag cgttccacca
    10021 cgttcagcga ctggctgcgg gtaaaacagc gggctgaggt ctgtttttcc tgacgggaga
    10081 acgactgcta ggattcggga atatacagcg acgaaggctg acgtggatcg aagggaatcg
    10141 ccccgaccat caccggattt ttgatgccct gcgctttggc atcggcaaac agcgcggcga
    10201 gtttttgctg gaagggactg tcgggcgaat ccccgttcac agccggttca tcgaaacggg
    10261 cgaaacatcc tgacgtcgta aaactgcggt acggcgacat aaagaaaaag cgattgggcg
    10321 caagtgttgc catggtctgc tgtacttcct cagccagtga cgtatccata tcatcctcca
    10381 caaaatgata aaggctttaa taatgattat cacttatatt ttcggccgct aacctaaaag
    10441 caaacgcgca ctatgtcaac tcttgaggta acgcaatctg caactcgggg ttgcatctgg
    10501 ttgtggttac aatgaaaatg agaagcatta ttgatgaatt cgcataagcg caatgtgatg
    10561 tcctgcgccg ttttgccccc tctccccaaa gggacggatt gtgcgctttg tcgaatttgt
    10621 cattacgccc ttaaccttat taataacagg aagctgactt gtgagactcg ccccgctcta
    10681 ccgcaacgcc cttctattaa caggactttt gctttcagga atagccgtag ttcaggccgc
    10741 cgactggccg cgtcagatta ctgacagccg gggcactcat accctggaaa gccagccgca
    10801 gcgtattgtt tccaccagcg tcaccctgac cggatcactg ctggcgattg atgctccggt
    10861 gatcgccagc ggcgcgacca cgccgaataa ccgcgtcgca gatgaccagg gctttttacg
    10921 ccagtggagc aaggtggcga aagaacgcaa actgcaacgg ctctatatcg gcgaaccgag
    10981 cgccgaagcc gttgccgcgc aaatgccgga tctgatttta attagcgcaa ccggcgggga
    11041 ttcggcgctg gcgctgtatg atcagctttc caccatcgcc ccgacattaa tcatcaatta
    11101 cgacgacaaa agctggcagt cgctgttaac gcagcttggc gaaattaccg ggcatgagaa
    11161 acaagcggca gagcggattg cacagtttga taagcaactg gcagcggcga aagagcaaat
    11221 caaattaccg ccgcagccgg tcactgccat tgtctatacc gccgctgcac acagtgccaa
    11281 tctctggacg ccggaatcag cacaagggca gatgctggaa caactcggct ttacgctggc
    11341 gaaattgccc gcagggttaa acgccagcca aagccagggc aaacgccatg acatcattca
    11401 gcttggtggg gaaaatctgg ctgcggggtt aaacggtgaa tcgctattcc tgttcgctgg
    11461 tgatcagaaa gatgccgatg cgatttatgc caatccactg ctcgcgcacc tgcctgcggt
    11521 acaaaacaag caggtttatg cgctgggaac cgagacgttc cgcctggatt actacagcgc
    11581 catgcaagtg ctggaacggc ttaaggcgct gttttaagca ttaactgtcg gacgctgtca
    11641 cctgcggcgg cgtctggcga aaacgtcgca actccaccag caccagcaat aacaacacgc
    11701 cgataatcaa caaaccaaaa ccgctcgcgc ttgcggaagc aaccggtgtc atcatcgcgc
    11761 ccaaaccacc cagcagcgcc gcgcctatgg catcgcccgt cacgttctgc gccgtccaca
    11821 aaccgttaat ccgccctaac atcgcttccg gggtttgcgt ttgcagcatt gtatattgca
    11881 gcaacgaact aaccgcgctc agccagccga acagcgccag acaaaccacg cctaaaatcc
    11941 acatcggcat caggccaaac agaccaatgg cgaggaacga tcccagcgtg gagagcagca
    12001 tcaataaccc tggtcgcgca ctatgtgcca gcttcccgct ggttaacgca ccaatagccg
    12061 cgccgagcgg gatcgccgcg tagagaaaac caatctgtgc cgctgacatc tgccagttgt
    12121 cagccagcgc cggatacagt accagcaccg cgctcgccat cgtcaataaa ccacccagca
    12181 gcgggctggc aagcagaaaa cgaaatcctg ccagtaatga tttcaacgga tgctcaagcg
    12241 gctgcggtgg cggtggcaac tccggaaggc ttaataacgg taacaaggta ataaacgtgc
    12301 ccgccgccgc cagcccgtag ttccaggcta cgccgccggt cgccagcaat aaaccgccaa
    12361 tcatgggcga aatcaccgac cccaggcgca cggtcaacat ggtgatcgcc ccggcctgca
    12421 ttaagttttc acgccctacc agtgcagatg ttgccgccag tagcgccgta acaccaagtg
    12481 atgcgaaaaa accatcccat aaaccaagta aatagattgc cagcaatgac ggctccggca
    12541 gcagtgcatt aaggcacagt ccaatgaagc caatgccaca ggtgccgcgc gccagcaaaa
    12601 tcactttttt gcgttcatag cgatccgcca gcacaccgcc aaccatcagg ccaacaaaca
    12661 tcgcgccgcc ggtcagcgtc accgaaagcc ccacctgcca ggtagaatgg gtcatcatct
    12721 ggatctgcac cggcaccgcg acgccgagca aacccagaga cacaattgag ataaaacgag
    12781 cgaggaatac tgcgcgaaac gccgggtgcg ttttcaacag gctgaggtta agcagccagg
    12841 attgtttatt cattacaatg ccttgccatc aaatatttta aatacctatt cttaggctgc
    12901 acatgttaac atatccaaat aagatcgata acgataatta atttcattat catggaagtt
    12961 cgtatatctg gttctgttgc cgtgacacgc gccattgccg tgcccggatt gctgttatta
    13021 ctgattatcg cgacggcatt aagcctgctc attggggcaa aatcactccc cgcttccgta
    13081 gtgctggagg ccttctccgg cacctgccag agcgccgact gcaccatcgt gctcgacgcg
    13141 cggctgccgc gtacccttgc cggtttactg gcaggcggcg cgcttggcct tgccggggcg
    13201 ttaatgcaaa ccctcacccg aaacccactt gccgaccccg gcttgcttgg cgtgaacgcc
    13261 ggagccagct ttgccattgt gctgggtgcg gcgctgtttg gttactcatc cgcacaggaa
    13321 caactggcga tggccttcgc cggggcgctg gtggcctcat tgattgttgc ctttaccggc
    13381 agtcagggtg gagggcagtt aagtccggtg cgtttaaccc tggcgggcgt ggcgctggcg
    13441 gcggtgctgg aaggactgac cagcggcatc gccctgctta atcctgacgt ctacgatcaa
    13501 ctgcgtttct ggcaagccgg ttcgctggat attcgcaatc tacatacctt aaaagtggtg
    13561 ctgatcccgg ttctgatcgc cggagcaact gcgctattac tgagtcgcgc gctgaacagt
    13621 ttgagcctcg gcagtgacac cgcgacggcg ctgggcagtc gcgtggcgcg cacacagttg
    13681 attggtctgc tggcgattac cgtgctttgt ggtagtgcga cggcggtagt tggcccgatt
    13741 gcctttatcg gcctgatgat gccgcacatg gcgcgctggc tggtaggtgc cgatcatcgc
    13801 tggtcgctgc ccgtcacgct acttgctacc cctgccctgc tgctgtttgc cgatatcatc
    13861 gggcgggtga ttgtgcccgg cgaactgcgc gtttctgtgg tcagtgcgtt tattggtgca
    13921 ccggtgctga tcttcctcgt gcgacgtaaa acgcgaggtg gtgcatgatt tacgtctctc
    13981 gccgattact catcacctgt ttgctgctga tttccgcctg tgtggttgca ggtatctggg
    14041 gattacgcag cggtgccgtc acgctggaaa tctcgcaggt attcgccgcg ttgatgggcg
    14101 atgcgccgcg cagtatgacg atggtggtca ccgaatggcg tttaccacgc gtgctgatgg
    14161 cgctgttgat tggcgcggcg ctgggcgtca gcggcgcgat ttttcagtcg ctgatgcgta
    14221 acccgctcgg cagccctgac gtaatgggct ttaacaccgg ggcgtggagc ggcgtgctgg
    14281 tggcgatggt gctgtttggt caggatctga cggctatcgc gctggcagca atggtgggcg
    14341 gcattatcac ttcgctgttg gtctggctgc tcgcctggcg taacggtatc gacacctttc
    14401 ggttgattat tatcggtatc ggcgttcgcg ccatgctggt ggcctttaat acctggctgt
    14461 tgctgaaagc gtctttagaa acggcgctaa cagcaggttt gtggaatgcc ggatcgctca
    14521 acggcctgac gtgggcaaaa acctcgcctt ccgcacccat cattatattg atgctcattg
    14581 ccgccacctt gctggtgcgg cgaatgcgct tgctggagat gggcgatgat accgcctgcg
    14641 cgctgggcgt cagcgtcgag cgttcgcgtc tgttaatgat gctggttgca gtggtgctta
    14701 ccgctgcggc aacggcgctt gccgggccga tttcctttat tgctttagtc gcgccgcaca
    14761 ttgcccgacg cattagcggc accgctcgct gggggctgac ccaggcggcg ctgtgcggag
    14821 cgctgttact gctggcggcc gttctctgcg cccaacaact gtttatgccg tatcaacttc
    14881 cggttggcgt cgttaccgtc agcctcggcg gtatttacct tatcgtcttg ttaattcagg
    14941 agtctcgcaa aaaatgaccg aatcagtagc ccgtttgcgc ggcgaacagt taaccctggg
    15001 atatggcaaa tataccgttg cggaaaatct gactgtagaa attcctgatg gtcacttcac
    15061 ggcaattatc ggaccaaatg gctgtggtaa atccacgtta ctgcgtacct taagccgcct
    15121 gatgacgcct gctcacgggc atgtctggct ggatggcgag cacattcaac attacgccag
    15181 taaagaagtt gcacgccgga ttggtctgtt ggcgcaaaat gctaccacgc cgggcgatat
    15241 caccgtgcag gagctggtgg cgcgtggacg ttatccgcat caaccgctgt ttacccgctg
    15301 gcgcaaagag gatgaagaag cggtaacgaa agcgatgcag gccacgggaa taactcatct
    15361 ggcagatcaa agcgtggata ccctttctgg cggacaacgc cagcgagcgt ggatcgcgat
    15421 ggtgctggcc caggaaacgg caattatgct gctcgacgaa cccacgacct ggctggatat
    15481 cagtcatcag attgatctac tggaactgtt aagcgaactg aaccgcgaga aaggctatac
    15541 cctggcggcg gtgctgcacg atcttaatca ggcctgtcgt tacgccagcc atttgattgc
    15601 attgcgggaa gggaaaattg ttgctcaggg agcgccgaag gagattgtca ctgctgaact
    15661 gattgagcgc atttatggtc tgcgatgcat gatcattgac gatccggtag ccggaatgcc
    15721 gcttgtggtg ccgctcggac gaacggcacc ttcaaccgca aatagttaaa ctaagtggtc
    15781 tgccatcatg gcatcctgtt ttctggatgc catcgcatag cgcaataaca cgctaccaca
    15841 agccaccatc ccgccgatca gcgcggaaag gatcacaata atcgccttac ccggaccgtc
    15901 ttttttcact ggcaatgacg ggcttaactg atatttaaac ggcgtaaaat tcacatcgtt
    15961 gacatttgct tttgttaatt gctcgacaag atactgccga ttacgtaatt caccgttcag
    16021 ttccgcaacg tcagtaaccg ctttttcaat ttccagtttg cgttcaatac cgtctgcgcc
    16081 gagggaaata gagaaatcgg ggtcatcttt aacggcctga ccattactgt atacgggctt
    16141 tttaattcct gccgcgttgg caatgtcgag tgaataattg aggcgctgaa tgtttgcatc
    16201 aagttgattt ttcgttttaa tgcgatcctg agccagtttt tctttttcaa actgggtttt
    16261 gatctccagt ttattacgga cgttttctat cgactctttc accaccaacg cagagatata
    16321 atcgatatac ccgctcaaaa cggtctgcgc ctctttactg gttggcgcgg taaaacttag
    16381 cgtccaggat gtatacagtg acggttcatc ttttttctta ctagcattgt catcaaccgc
    16441 tttcattttt tcgctcaatg caacaatagc gcgatgcaaa tccagttcat cgattttcgc
    16501 ctcttttaat tagtccatca cataaggtga tgaacgcagg tactcttcca gcaagctaac
    16561 agactgaaac ttcttgataa acaggttaaa tgcaccgcca cggtcgatat taacatcgag
    16621 atcaagaacg cggagtttgg taaaggtttt ctccaggtcc tgccactgaa tggcttcggc
    16681 gggagtaata acggctgagc tggtccattt ttgcggcagg atgaaagaga tcagtaaacc
    16741 tgcgcaggca aatgcaaaaa cgaccgccat gaccgttttt ttggcccgcc ataaaacttc
    16801 gattagatta agtaaatcaa tttcattatt actgggcgac gccagaggat actcagaaaa
    16861 atgagcgtca cttccctgtt taatattcaa tgatgacatg gggtataacc tgaaaatgag
    16921 tccaataggg gaaaatctcc acggcgctca ctttaccagt tggtgagaat tatccgaagc
    16981 tgaagtttgt aaattccttc cactgaatgc aacattcact ctatttaaga gaaatattca
    17041 tcaaacctat aaatcaaaca aataaaaaac ccataattat tttctgtaat
//
LOCUS       DAJTSF010000085        16388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000085 DAJTSF010000000
VERSION     DAJTSF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.085"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO335_002977"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO335_002977"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6003280.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO335_002978"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO335_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6003281.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2339..2554
                     /locus_tag="OO335_002979"
     CDS             2339..2554
                     /locus_tag="OO335_002979"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003282.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="OO335_002980"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="OO335_002980"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6003283.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /gene="galP"
                     /locus_tag="OO335_002981"
     CDS             4449..5843
                     /gene="galP"
                     /locus_tag="OO335_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6003284.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="OO335_002982"
     CDS             5920..6417
                     /locus_tag="OO335_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6003285.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="OO335_002983"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="OO335_002983"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6003286.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="OO335_002984"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="OO335_002984"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6003287.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="OO335_002985"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="OO335_002985"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6003288.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9102..9665
                     /locus_tag="OO335_002986"
     CDS             9102..9665
                     /locus_tag="OO335_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6003289.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="OO335_002987"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="OO335_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6003290.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="OO335_002988"
     CDS             complement(10257..11237)
                     /locus_tag="OO335_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6003291.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="OO335_002989"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="OO335_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6003292.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="OO335_002990"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="OO335_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6003293.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /locus_tag="OO335_002991"
     CDS             12540..12830
                     /locus_tag="OO335_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6003294.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="OO335_002992"
     CDS             12838..13431
                     /locus_tag="OO335_002992"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6003295.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="OO335_002993"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="OO335_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6003296.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14874..15837
                     /locus_tag="OO335_002994"
                     /pseudo
     CDS             14874..15837
                     /locus_tag="OO335_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15882..16385
                     /locus_tag="OO335_002995"
     CDS             15882..16385
                     /locus_tag="OO335_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR6003297.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4058 a         4220 c         4124 g         3986 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct
    12061 cattgtgatt tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaataaatat cgacgacatt tattatatat atttagtgaa tatttactaa tcattcattg
    14701 attataaata tgtacaacct ggcgcacaaa tttaatattt aatttctgac tttatttcaa
    14761 gttgtgataa acatagacaa gttgtcagac aggttaacca atttgtcgca caaatcactg
    14821 ataagcacaa ttagatatca accaatttcc atccttcaac agggagtttt tttatgaaaa
    14881 tgaggtcatt tacccgctca ttagtctgcg catccttgct ggctctggtt agcactggcg
    14941 taaacgccgc agaaaaagtg gcgttaaagt tagcacacaa cctggaaaga agtcatgttg
    15001 tacatcagag ttttgaagag ctggcgaaag aggtaaaaca gctgtcaaaa ggtaatatgg
    15061 tgatccgtat ttatcccagc agccagatgg gtaatgcgcg agaaactatg gaattgttgc
    15121 aaaatggcgc actggatatg accaaaggtt ccgccagtga tctggagtct tttgataata
    15181 tttatgctat ctataatttg ccgttcttat taagttgttt ttggtgaagt tggtaaagaa
    15241 attatggact caacaaaaga caaaggcttt tttgctttat cagcttatgt agcgggtact
    15301 cgcagtttct atgcgaaaaa accgattaca aagccagaag atcttaaagg actgaaaatt
    15361 cgtgttcagc caagtccgac caccatcaaa atgattgaac tgatgggtgg ttcacccact
    15421 ccaatttcat ttggcgaagt ttatactgcg atgcagcaag gtgtagtaga tggcgcagaa
    15481 aataacgtgc cttcctggat gcagactcga catattgaaa ttgccaaagt tttttctgaa
    15541 gatgaacacg cctcaattcc tgactttttg gttatctcca ccaaaacatg gaataaatta
    15601 accccagaac agcaacaaat cctcgaaaca gcggcgaaga aatccgaagc ttatcaacaa
    15661 aaattgtggg aaaaaattga cgctgacaca cgtgctcagg cgaaagctat ggggggtgaa
    15721 attgtaaaag tggataaagc gccattccgt aaagccgtgc aacctctgtt tgacgacttc
    15781 aaaaaagatc caaaacaagc tgctttgtta gagaaattcg acaacgctgc tcaataatat
    15841 tccgggccag cctctggccc tgattttcca ggatgcttac aatgatattt aaccgcctga
    15901 aacttgcggt agaccgcgtc attgccacgt tttctgtcgc cgtaatgctt gctctggttg
    15961 tatgcgtagt ctggcaagta tttagccgct acgtacttaa ccagccaagt acattaaccg
    16021 atgagctggc acgctttttg atgatctggg taggattatt aggtgctgca tataccgtcg
    16081 gcgcgcagcg acatctctct attgatttat ttgctctcgc cctgaataag cgcaaacaat
    16141 tacttcttag tatagtcatt aacgttctca ttttaggttt tgccggttcg gtcattgtga
    16201 ctggtggcct gaaattaatt gataaaacac tggcaacatc acaggtatct gccgctatgc
    16261 aaataccgat gggatacgta tatatcattc tgccattaag tggagtggta atgatgtttt
    16321 atgccttgtg ttttattaat caaagcattc aacaactgaa acaacctgcg caggaggcca
    16381 gttaatgg
//
LOCUS       DAJTSF010000086        15930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000086 DAJTSF010000000
VERSION     DAJTSF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.086"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="OO335_002996"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="OO335_002996"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6003298.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="OO335_002997"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="OO335_002997"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6003299.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="OO335_002998"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="OO335_002998"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6003300.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="OO335_002999"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="OO335_002999"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6003301.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4817
                     /gene="citX"
                     /locus_tag="OO335_003000"
                     /pseudo
     CDS             4267..4817
                     /gene="citX"
                     /locus_tag="OO335_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4792..5670
                     /gene="citG"
                     /locus_tag="OO335_003001"
     CDS             4792..5670
                     /gene="citG"
                     /locus_tag="OO335_003001"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR6003302.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5721..7183
                     /gene="citT"
                     /locus_tag="OO335_003002"
                     /pseudo
     CDS             5721..7183
                     /gene="citT"
                     /locus_tag="OO335_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7297..8103
                     /gene="rna"
                     /locus_tag="OO335_003003"
     CDS             7297..8103
                     /gene="rna"
                     /locus_tag="OO335_003003"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6003303.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8332..8742
                     /gene="rnk"
                     /locus_tag="OO335_003004"
     CDS             8332..8742
                     /gene="rnk"
                     /locus_tag="OO335_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6003304.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8973..10211)
                     /locus_tag="OO335_003005"
     CDS             complement(8973..10211)
                     /locus_tag="OO335_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR6003305.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10432..10860
                     /gene="uspG"
                     /locus_tag="OO335_003006"
     CDS             10432..10860
                     /gene="uspG"
                     /locus_tag="OO335_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6003306.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="OO335_003007"
     CDS             complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="OO335_003007"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6003307.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="OO335_003008"
     CDS             complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="OO335_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6003308.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13726..14472
                     /gene="dsbG"
                     /locus_tag="OO335_003009"
     CDS             13726..14472
                     /gene="dsbG"
                     /locus_tag="OO335_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR6003309.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14682..15584
                     /locus_tag="OO335_003010"
     CDS             14682..15584
                     /locus_tag="OO335_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6003310.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15731..15930
                     /locus_tag="OO335_003011"
                     /pseudo
     CDS             15731..15930
                     /locus_tag="OO335_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3954 a         4118 c         3854 g         4004 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcaaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ccacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgcgcc tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc gcgcgtggaa
     4741 aaacccatca actgaccgat ttactcaacc gcatggaggc actgctgaac gatgtcgatg
     4801 cctgcaacgt caactaaaac cacaaagctt gcgacgtcat taatcgatga gtacgccctg
     4861 ctgggctggc gcgccatgct gactgaagtc aatctgtcac cgaaaccagg cctcgtggat
     4921 cgcattaact gcggtgcgca caaagatatg gcgctggaag atttccaccg cagcgcgctg
     4981 gcgattcagg gctggttacc ccgtttcatt gaatttggtg cctgtagtgc agaaatggca
     5041 ccggaagcgg tactccacgg attacgccca attggtatgg cttgcgaagg tgatatgttc
     5101 cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt tttctttagg gctgctatgt
     5161 gcggcaattg gccgtttgct tcagctcaac cagccggtta cgccaacaac cgtttgttct
     5221 acggcggcaa gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa taattcacga
     5281 ctgacggcag gtcaacggtt gtaccagcag cttggcctta ccggcgcacg cggtgaagcc
     5341 gaagcaggtt atccactggt gatcaaccac gccttgccgc attacctcac tctgctggat
     5401 caggggttag atcctgaact ggcattgctc gataccttgc tcctactgat ggctaccaac
     5461 ggcgatacca acgttgcatc gcgcggtggc gaggggggcc tgcgctggct acagcgcgag
     5521 gcgcaaacat tattgcaaaa agggggcatt cgaaccccca ccgatctcga ttatctccgg
     5581 cagttcgaca gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc cgacctgctg
     5641 atccttacct gttttttagc acagatttaa ttatttaagc acttgataaa tttggaaata
     5701 ttaattttcg gagaacccgt atgtctttag caaaagataa tatatggaaa ctattggccc
     5761 cactggtggt gatgggtgtc atgtttctta tccctgtccc cgacggtatg ccgccgcagg
     5821 catggcatta cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc gagccaattc
     5881 cggcaacagc gatcagtttt attgcggtta ctatttgcgt tattggcagt aattacctgc
     5941 tctttgatgc caaagaatta gctgacccag cgtttaatgc gcaaaaacag gcgctgaaat
     6001 ggggcctggc tggtttttcc agcaccacgg tatggctggt atttggcgca tttatttttg
     6061 cattagggta tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg gtgaaattca
     6121 tggacaaacg cacgctgacg ttgggttatg cgattgtcat tatcgacatt ctgctggcac
     6181 cgtttacacc gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc attaaaaacc
     6241 tgccgccgtt gtttaaatca ttcccgaacg atccgtccgc gcgtcgtatt ggcggctatt
     6301 tgatgtggat gatggtcatt agtaccagtc tgagttcgtc catgtttgtc accggtgcgg
     6361 accaaacgtg ctgggtctgg agttcgtcag caaaattgcc ggtatccaga ttagctggtt
     6421 gcagtggttc ctctgcttcc tgccggttgg ggttatcttg cttatcattg cgccgtggct
     6481 ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa cctgggcggg
     6541 tgatgaacta aaaaccatgg gtgcgctgac acgcagagag tggacgctga ttggccttgt
     6601 attgctcagc ttaggtttgt gggtatttgg cagtgaagtc attaatgcta ctgcggttgg
     6661 tctgctggca gtttcgctaa tgctggctct gcacgttgtg ccgtggaaag acattacccg
     6721 ctataacagc gcatggaaca cgctggtcaa cctggcaact ctggttgtga tggctaacgg
     6781 cctgactcgt tctggtttta ttgactggtt cgccggtacc atgagtacgc acctggaagg
     6841 attctcacca aacgcaacgg tgattgtact ggttctggtg ttctactttg cacactacct
     6901 gtttgccagc ctgtctgcgc acaccgcaac catgctgccg gttattctgg ccgtcggtaa
     6961 aggtattccg ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt ctatcggtat
     7021 catgggctgt ctgacgccgt atgcaaccgg tcctggggtg attatttacg gctgtggcta
     7081 tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc ggggtgattt acatctctat
     7141 gttgctgttg gttggctggc cgattctcgc catgtggaac taatacgttc gccaaacgct
     7201 gaataaatca tcacgcccgc caggtaagcc cacctggcgg gcttttttat gatttaatag
     7261 atagtattca gtgtatgtat gagttccaca cccattatga aagcattctg ccgtaacgcc
     7321 gcgttgctcg cggtttctct gcttccctta tcttctgcca acgccgtagc gttgcaggca
     7381 aaacagtatg gcgattttga tcgctatgtc ctggccctct cctggcaaac cggattttgc
     7441 cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca aaccgaaacg
     7501 accaacaaaa ctgattttct gaccgtacat ggtctgtggc caggattgcc taaatcggtt
     7561 gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg gttgcgctac tcgcccaatc
     7621 ccgaatctac cagaagcacg cgccagccga atgtgttcat cgccggaaac cgggttatca
     7681 ctggaaacgg ccgctaaact aagtgaagtc atgccaggag ctggcggacg ttcctgcctg
     7741 gaacgctacg aatatgccaa acacggtgcc tgctttggtt ttgatccgga cgcatacttc
     7801 ggtacgatgg tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa attccttgcg
     7861 gataattacg gtaaaacagt gagccgccgt gactttgacg ccgcctttgc caaaagctgg
     7921 ggaaaagaga acgtgaaagc agttaagcta acgtgccagg gtaaccctgc gtatttgact
     7981 gaaattcaga tctcgatcaa agctgacgcc atcaacgctc cgctttctgc aaactcattc
     8041 ttgccacaac ctcacccagg taactgtggc aaaacctttg tgattgataa agcgggttat
     8101 taatcctctg atttacaaaa cacgctaatt cgtcttccat aaccatgccg attaagaatt
     8161 atacataatg cgctgcattg aggaattccc tgaatgtgac gcaaatcact tcaaagagtg
     8221 agcaacagtc agtatcatca tgtgagttaa accctcgccg cctgacggtg agggttttct
     8281 tttgggtatg tttatctgcg acactcgcag taccgacgac atggagtaaa aatgtccaga
     8341 ccaactatca tcattaacga cctggatgcc gaacgcatcg atattctgct ggagcaaccc
     8401 gcctatgctg gtttgccaat cgccgacgcg ttaaacgcag agttggatcg cgcccaaatg
     8461 tgttcgccag aagagatgcc acacgacgtg gtgacaatga acagccgggt taaattccgc
     8521 aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa aatgaccgat
     8581 agcaatactc agctttccgt tatggctccg gtaggtgccg cactgctggg gctgcgcgtt
     8641 ggcgattcca ttcactggga acttccgggc ggcgttgcaa cccaccttga agtgctggaa
     8701 ctcgaatacc agccagaagc tgctggcgac tacctgcttt aatcccatct gcacaagcga
     8761 taactcgctt tgcccagagg atgcactcgc atcctcttta cgcaaaatca gcaatttttc
     8821 ctggttctca ctgttcactt ttgctgcaaa attattccgt ttcgtttgtg ggaataacca
     8881 tggcagaagc attttacatc ctgataggat ttctgattat ggcggcgatt atcgtcatgg
     8941 ctgtccttta cctcgaaaac cactcctgac gatcatattg ttccccccgg catcgcattc
     9001 accagacctg aaaccgcctt ctgcgccgcc tctgcgcttt gtgcaccggg taccagaatc
     9061 accttacggc actcctcttc acgtttttcg aaaatctcat atccccgggc ggcctcttca
     9121 aacggcatat agtgggtaac aatttcttct ggtttcagta atcctttctc aattaacggt
     9181 aataattctc ccagccatgc gtgaacgtgg gtctgtccca ttttaaacgt caacccttta
     9241 tcaaaggcgt cgccaaacag gaaaccgtga ataaatccag cgtagacgcc cggtacgcta
     9301 acaatgccgc cacgcctgac cgccgcaata cactggcgca acgctttacc gctgctgccc
     9361 tccagtttca ggttagtcag caccgtttcc gtggtgctgc ctttcgcttc aaaaccgacg
     9421 gcgtctatta ctgcatccac gccccggtga cctgccgttt gttcaataat tgactgtgcc
     9481 ggatcgctat cttcatcgaa attaatcggg atcgcgccgt agcggtcagc ggcgaaacgc
     9541 aagcggtagg gatgatgatc aacaacaaaa atctgttccg caccgagcaa ccgtgcacag
     9601 gcgattgtca acaatcccac aggaccagca ccatagactg caacgcttga accttgttgg
     9661 atctgcacat tttttgctgc ctgccatgcc gttggcagaa tatcagaaag gaaaagggct
     9721 ttatcatctg aaagcaaagg cggtacttta aacggcccca cattcccttt cgggacgcgg
     9781 acatattccg cctgcccgcc aggaacgcca ccatacaggt gactataacc aaacaatgcc
     9841 gcgggagcag gtatctgttt tttattgagc gcagcgcctt tacccgcatt ggtattttcg
     9901 caggcggcat attgctgtaa tcgacagaaa aaacagtcgc cacaagcaat gacgaacgga
     9961 attaccaccc ggtcgccttt ttgcaaattt tttacgtcct tcccggtttc aacgacttcc
    10021 cccataaatt catgaccaaa aatatcgcca tgtttaacct ggggtatttt gcctcgataa
    10081 agatggaggt cagagccaca gatcgccgtt accgtaatac gcagaataat atcatctgcc
    10141 tgttcaatgc ccggatcggg aacattttct acctgaacgt gatgtgggcc gtgataagtc
    10201 aatgctttca tgcaacctcc agagacagtt caaaaaagag tcagtcttta agggtagcta
    10261 tcacagcggg aaaacgggcg gtaccgcagg ttttgggaga attcctagtg ttcagtgaga
    10321 atatattaat cacagaaacc gttcgtaagg aaaaagaacc agacaaatat cagggataaa
    10381 tattataatc atggagaatg atttgttcat gattaacagg gagaaagggt tatgtataag
    10441 gcaatcatta tgccagttga tgtatttgaa atggaattga gcgacaaagc tgttcgccac
    10501 gctgaattcc tcgcccagga tgacggagtt attcatctac ttcacgtact acccgggtca
    10561 gccagcctga gcctgcaccg ttttgccgct gatgtgcgtc gttttgaaga gcatctgcaa
    10621 catgaagcag aagaacgtct gcaaacgatg gtcagccact tcaccatcga tccttcccgc
    10681 attaaacaac atgtccgttt tggtagcgtg cgggatgaag tcaatgagtt ggcagaagaa
    10741 ctgggggctg atgttgtagt tatcggttct cgcaacccat cgatttcgac ccatctgtta
    10801 ggttctaacg cctcgagcgt gatccgccac gccaatctgc ctgtactggt cgttcgctaa
    10861 cagataaaag tatttatgcc gccctgcctg gaacatcccg gcagggcttt ttaatgccat
    10921 aaaaaagccg ccaggtttga ccccggcggc taagcaattg caggtgaatc ttacttcttc
    10981 ttatgcagtt ttggtgcgaa tcaggtagtc aaaagcactc agagaggctt tggcaccttc
    11041 gccagtggcg atgatgatct gcttgtacgg aaccgtcgta cagtcacccg ctgcgaacac
    11101 gcctttgacg ttggtttcac atttcgcatc aatgataatc tcgcccatgc ggttacgttc
    11161 gactgcgcct tccagccagt tggtgttcgg cagcagacca atctggacaa aaataccggc
    11221 cagttcgatg ttgtgaatat tgccgctgac acgatctcga tattccagac caacgacttt
    11281 gctgccgtcg cctttcactt ccgtggtttg cgcattcaga ataatgtcga cgtttttcag
    11341 gctgcgcagt ttgtcctgca gaacctggtc ggctttcatt tctggcgcaa attccagcag
    11401 cgttacgtgc tcaacgatac ccgccaggtc gattgccgct tccacgccgg agttaccgcc
    11461 gccgataacc gctacgcgtt tgcctttaaa cagcgggccg tcgcagtgcg ggcagtaggt
    11521 cacgccttta gtgcgatact gatcttcgcc cggaacgttc atgttgcgcc attttgcacc
    11581 cgtcgccacg ataatgctgc gtgctttcag taccgcgcca gaagctgttt caatctggtg
    11641 tagaccacct tcaaccgctg ccgggatcag tttgctggcg ctctggctgt cgatcacatc
    11701 aacgtcgtat tcatcaacgt gaactttcag tgcgcctgcc aacttctgcc cttcagtctt
    11761 cggtacagaa atgtagtttt cgatatcaac ggtatcgagg atctgaccac caaaacgttc
    11821 gcccatcaga ccggtacgga tgcctttacg tgcggagtaa attgctgccg ctgcacccgc
    11881 cgggccggaa ccaacgatta atacgtcata agcatcacgc ttgttcagct cttctgccgc
    11941 acgattttcc gcgccagtat caattttggc aacgatttca gtcaacgtca tgcggccctg
    12001 accaaactct ttcccgttta cgaacactgc cggaacgccc atcacgttgc gatcggtgat
    12061 ttcgttctgg aaggtgccgc cgtcaattgc agtgtgcttg atgcgcgggt tcaatacgct
    12121 catcaggttc agcgcctgca ccacgtccgg gcagttatgg caagagagcg agtaataggt
    12181 ttcgaattca aaatcaccgt caatatggcg aatctgctcc agcagagact gcgcttcttt
    12241 cgacggatga ccaccggtcc acagcaacgc cagtaccagc gaggtgaact cgtggcccag
    12301 cggggagcct gcaaaacgtg gtccctggtt ggaacctggg ttggtgatca ggaaagacgg
    12361 cttacgcact ggcaagctgt tatcttcttt aaaggtgact ttgtctgaca gttctgcgat
    12421 ttcagccaac agttccttga tttctgccga tttagcgctg tcatccagcg tggcaattaa
    12481 ctcaacaggc ttggtcaatt tctcaaggta agccttgagt tgagttttca tatttgtgtc
    12541 gagcatgttt atctcctggg ctttaaacat catcatgcaa gcggcctctg cggctacctg
    12601 gatgcagctt gcatcatggt gcggggaaga aaatcgggtg ccgcagcacc cgaagaatta
    12661 ggtgaatttc cgaagtattt cgcgtcagag caaggcgact agcctgtgaa tccccgggag
    12721 cttacacaag taagtgaccg gggtgagcgg gcgacgccaa cgccgctatg gcgtgaaaga
    12781 cgaaggaaat ttagatttta ccaaccaggt ccagagacgg agccagagtt gcttcacctt
    12841 ctttccattt agccgggcaa acttcacctg ggtgagaagc tacgtactga gctgctttga
    12901 ttttacgcag cagatcagac gcgtcacggc caatgccttc agcggtaact tcgattgcct
    12961 ggatgatacc ctgcgggtca acaacgaagg tcgcacggtc agccagacct tcatcttcac
    13021 gcatgttgtc gaagttacgg gtcagggcgc cagtcgggtc gccgatcatc gcatatttga
    13081 ttttagcgat ggtttcagag ctgctgtgcc atgctttgtg ggtgaagtga gtatcggtag
    13141 atactgcgta tacgtctacg cccagtttct gcagttcttc gtagtggtca gcaacgtcac
    13201 ccagttcggt cgggcatacg aaagtaaagt cagccgggta gaagaagaag acgctccagc
    13261 ggccttcggt atctttttcg gtgatttcga tgaattcgcc gtttttgaat gcctggtttt
    13321 taaaaggttt aattttggtg ttaatcaagg acatctatac ttcctccgtg ttttcgatga
    13381 gatgtaaggt aaccaatttt tgccgattcg gctaatgcgt ttccattatc aaatcgataa
    13441 gttttacctt acaaccttcg taagacaact ttgtgaactt tgcaccgcca gcggcgataa
    13501 aaagtaaaat ggcccccttt ccaggtgacc gtgtaatctg aactaccctt tggtatcttc
    13561 agcgcctgcc tgcaggctga ggtgttgccc ttacaaatgc aacaacgaca tggattacaa
    13621 caccctcata aacaaagggc aatcacctga tctaagctct tacctatgac agtgataggt
    13681 tatgcctttt actcgacttt tgcactgact gaaaagaaca aattaatgtt aaaaaagata
    13741 cttttactgg ctctgcttcc tgcaatcgcc ttcgcagagg aacttccttc cccagtaaaa
    13801 gcgattgaaa aacagggcat tacaatcatc aaaacgttcg atgcccccgg aggaatgaaa
    13861 ggttatctcg gaaagtatca ggatatgggc gtcaccatct acctgactcc cgatggtaag
    13921 cacgctatct ctggttacat gtacaacgag aaaggtgaaa acctgagtaa cacacttatc
    13981 gaaaaagaaa tttacgcacc agccggacgc gaaatgtggc aacggatgga acaatcccac
    14041 tggctcctcg acggtaaaaa agatgcgccg gtcattgtct acgtcttctc cgatccgttc
    14101 tgcccatatt gtaaacagtt ctggcagcag gcgcgcccgt gggtagattc tggcaaagtg
    14161 caattaagaa cattgttggt tggggttatc aagccagaaa gcccggcgac agcagcggca
    14221 attcttgcct ccaaagatcc cgcaaaaacc tggcaacaat atgaagcctc tggttgcaag
    14281 cttaagctaa acgtgcctgc aaacgtaagt acagagcaaa tgaaagtgtt aagtgacaat
    14341 gagaaactga tggacgatct gggggcaaat gtcacgccgg ctatctatta catgagtaag
    14401 gaaaatacgc tgcaacaggc cgtggggttg cccgatcaga aaacgcttaa tatcattatg
    14461 ggaaataaat aaaaggtaat tcatggatgg ttgaactata tccctgcttt tcaaccatcc
    14521 taaaaaagca tgaatttctc gctctaccct aagtggctgt gctattttag aaaaacttaa
    14581 tcctgagtta agcaaaaaaa cataatcaat aaaaatacat acgcaaaaaa cattgattaa
    14641 gtgaatatac catggaagaa aaatataaac cggagtagtg tatggccaat ctctacgact
    14701 tgaaaaagtt cgacttaaat cttcttgtca tatttgagtg tatttaccag catttaagca
    14761 ttagcaaagc tgctgaatca ctatatatca ccccatcagc cgtgagccag tcgttacaac
    14821 gcttacgtgc gcaatttaat gaccctttat tcattcgctc aggaaaaggt atcgcgccga
    14881 cgacaacagg gttgaatctg catcaccatc ttgagaaaaa cctcaaaggc cttgaacaaa
    14941 caattaatat agtcaataag tccgaactaa agaaaaactt tattatctac ggccctcagc
    15001 tcatttcttg ctctaataat agtatgctga tccgctgcct acggcaagat gcctctgtcg
    15061 agattgagtg ccatgatatc ctcatgtcag cagagaatgc tgaagaactg ttggttcacc
    15121 gcaaagccga tctggtgatc acccaaatgc cagtgatcag ccgttccgtc atttgcatgc
    15181 cattgcatac aattcgcaat actctaattt gtagtaataa gcatccgcga attactgaca
    15241 atagcactta tgaacaaatt atggcagaag agttcaccca attaatagcc aaatcagcag
    15301 gagttgatga tattcaaatg gaaatagacg agaagtttat gaatcgcaaa atttcatttc
    15361 gtggttcctc tttgctgaca ataattaata gcattgcagt tacagattta ctgggaatag
    15421 tcccctatga actttataac taccaccgtg actttcttaa tttaaaagag ataaaactag
    15481 aacatccgtt accttcaata aagctttaca tttcctataa taaatcttct ttaaataatt
    15541 tggtgttctc ccgatttatt gaccgtctaa atgagagttt ttgatatata actatagaca
    15601 gctactataa ctttcatcta tttattcaca gcgcgaatga aaaataaaac actctgcttt
    15661 acagtgttta ctttataatt aacagtctta tctcactgtt caccctgtaa cgttcagaga
    15721 gcgtcttcag atgtctattt ataaaattcc tcttccgctc aatattctgg aagctgcacg
    15781 agagcgtatt acctagacac tcaacaccct accccgcgtt tgtgtctcat tttctggcgg
    15841 caaagactct ggtttaatgc tgcatctgac agccgaactt gcccgacaaa tgggcaaaaa
    15901 aatctgcgtt ttgtttatca actgggaggc
//
LOCUS       DAJTSF010000087        15703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000087 DAJTSF010000000
VERSION     DAJTSF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.087"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..195
                     /gene="tuf"
                     /locus_tag="OO335_003012"
     CDS             <1..195
                     /gene="tuf"
                     /locus_tag="OO335_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6003311.1"
                     /translation="FYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLR
                     FAIREGGRTVGAGVVAKVLG"
     gene            378..572
                     /gene="bfd"
                     /locus_tag="OO335_003013"
     CDS             378..572
                     /gene="bfd"
                     /locus_tag="OO335_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR6003312.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            645..1121
                     /gene="bfr"
                     /locus_tag="OO335_003014"
     CDS             645..1121
                     /gene="bfr"
                     /locus_tag="OO335_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR6003313.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1118..1585)
                     /locus_tag="OO335_003015"
                     /pseudo
     CDS             complement(1118..1585)
                     /locus_tag="OO335_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1964..2275
                     /gene="rpsJ"
                     /locus_tag="OO335_003016"
     CDS             1964..2275
                     /gene="rpsJ"
                     /locus_tag="OO335_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6003314.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2308..2937
                     /gene="rplC"
                     /locus_tag="OO335_003017"
     CDS             2308..2937
                     /gene="rplC"
                     /locus_tag="OO335_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6003315.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2948..3553
                     /gene="rplD"
                     /locus_tag="OO335_003018"
     CDS             2948..3553
                     /gene="rplD"
                     /locus_tag="OO335_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6003316.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3550..3852
                     /gene="rplW"
                     /locus_tag="OO335_003019"
     CDS             3550..3852
                     /gene="rplW"
                     /locus_tag="OO335_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6003317.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3870..4691
                     /gene="rplB"
                     /locus_tag="OO335_003020"
     CDS             3870..4691
                     /gene="rplB"
                     /locus_tag="OO335_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6003318.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4708..4986
                     /gene="rpsS"
                     /locus_tag="OO335_003021"
     CDS             4708..4986
                     /gene="rpsS"
                     /locus_tag="OO335_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6003319.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5001..5333
                     /gene="rplV"
                     /locus_tag="OO335_003022"
     CDS             5001..5333
                     /gene="rplV"
                     /locus_tag="OO335_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6003320.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5351..6052
                     /gene="rpsC"
                     /locus_tag="OO335_003023"
     CDS             5351..6052
                     /gene="rpsC"
                     /locus_tag="OO335_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6003321.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6065..6475
                     /gene="rplP"
                     /locus_tag="OO335_003024"
     CDS             6065..6475
                     /gene="rplP"
                     /locus_tag="OO335_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6003322.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6475..6666
                     /gene="rpmC"
                     /locus_tag="OO335_003025"
     CDS             6475..6666
                     /gene="rpmC"
                     /locus_tag="OO335_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6003323.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6666..6920
                     /gene="rpsQ"
                     /locus_tag="OO335_003026"
     CDS             6666..6920
                     /gene="rpsQ"
                     /locus_tag="OO335_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6003324.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7085..7456
                     /gene="rplN"
                     /locus_tag="OO335_003027"
     CDS             7085..7456
                     /gene="rplN"
                     /locus_tag="OO335_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6003325.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7467..7781
                     /gene="rplX"
                     /locus_tag="OO335_003028"
     CDS             7467..7781
                     /gene="rplX"
                     /locus_tag="OO335_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6003326.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7796..8335
                     /gene="rplE"
                     /locus_tag="OO335_003029"
     CDS             7796..8335
                     /gene="rplE"
                     /locus_tag="OO335_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6003327.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8350..8655
                     /gene="rpsN"
                     /locus_tag="OO335_003030"
     CDS             8350..8655
                     /gene="rpsN"
                     /locus_tag="OO335_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6003328.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8689..9081
                     /gene="rpsH"
                     /locus_tag="OO335_003031"
     CDS             8689..9081
                     /gene="rpsH"
                     /locus_tag="OO335_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6003329.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9094..9627
                     /gene="rplF"
                     /locus_tag="OO335_003032"
     CDS             9094..9627
                     /gene="rplF"
                     /locus_tag="OO335_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6003330.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9637..9990
                     /gene="rplR"
                     /locus_tag="OO335_003033"
     CDS             9637..9990
                     /gene="rplR"
                     /locus_tag="OO335_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6003331.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10005..10508
                     /gene="rpsE"
                     /locus_tag="OO335_003034"
     CDS             10005..10508
                     /gene="rpsE"
                     /locus_tag="OO335_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6003332.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10512..10691
                     /gene="rpmD"
                     /locus_tag="OO335_003035"
     CDS             10512..10691
                     /gene="rpmD"
                     /locus_tag="OO335_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6003333.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10695..11129
                     /gene="rplO"
                     /locus_tag="OO335_003036"
     CDS             10695..11129
                     /gene="rplO"
                     /locus_tag="OO335_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6003334.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11137..12468
                     /gene="secY"
                     /locus_tag="OO335_003037"
     CDS             11137..12468
                     /gene="secY"
                     /locus_tag="OO335_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6003335.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12500..12616
                     /gene="rpmJ"
                     /locus_tag="OO335_003038"
     CDS             12500..12616
                     /gene="rpmJ"
                     /locus_tag="OO335_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6003336.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12763..13119
                     /gene="rpsM"
                     /locus_tag="OO335_003039"
     CDS             12763..13119
                     /gene="rpsM"
                     /locus_tag="OO335_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6003337.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13136..13525
                     /gene="rpsK"
                     /locus_tag="OO335_003040"
     CDS             13136..13525
                     /gene="rpsK"
                     /locus_tag="OO335_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6003338.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13559..14179
                     /gene="rpsD"
                     /locus_tag="OO335_003041"
     CDS             13559..14179
                     /gene="rpsD"
                     /locus_tag="OO335_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6003339.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14205..15194
                     /gene="rpoA"
                     /locus_tag="OO335_003042"
     CDS             14205..15194
                     /gene="rpoA"
                     /locus_tag="OO335_003042"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6003340.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15235..15618
                     /gene="rplQ"
                     /locus_tag="OO335_003043"
     CDS             15235..15618
                     /gene="rplQ"
                     /locus_tag="OO335_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6003341.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4043 a         3690 c         4183 g         3787 t
ORIGIN      
        1 ttctacttcc gtactactga cgtgactggt accatcgaac tgccggaagg cgtagagatg
       61 gtaatgccgg gcgacaacat caaaatggtt gttaccctga tccacccgat cgcgatggac
      121 gacggtctgc gtttcgcaat ccgtgaaggc ggccgtaccg ttggcgcggg cgttgttgct
      181 aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc ttcggcgccc
      241 tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc gctgaactat
      301 ggcgcaaata aaactgtgct attgtaatca taaccattct catttacact ttgtgcagaa
      361 tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc
      421 gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag tttattcctg
      481 tgggaaatca atgcggtaag tgcattcgcg ccgcgcgcga agtgatgcag gacgagttga
      541 tgcagatgcc agaatttaaa gagattgcct gaagcccacg cttatttttt gacatcctgc
      601 tagccccatc tacgctctaa tagtggaagc ggagggacta taaaatgaaa ggtgatgtta
      661 aaatcataaa ttatctcaat aaactattgg gaaatgagct tgtcgcaatc aatcagtatt
      721 ttctccatgc ccggatgttt aaaaactggg gcctgatgcg tctcaatgac gtcgaatacc
      781 atgaatccat cgatgagatg aaacacgctg ataaatatat cgagcgtatt ttgtttttag
      841 aaggaatccc caacttacag gatttaggca aactggggat tggtgaagat gtcgaggaga
      901 tgctgcaatc tgacctgcga ttagaactgg aaggcgcgaa ggatctgcgt gaggctatcg
      961 catatgccga cagcgttcat gactatgtta gccgcgatat gatgatcgag atccttgctg
     1021 atgaagaagg tcatatcgac tggctcgaaa ctgaactgga tctcatcggt aaaatcggcc
     1081 tacaaaatta cctgcaatca caaattaaag ttaaagatta acggcctatc agcaaactat
     1141 cccagcctat aacgaaaccc gcagccgcca gaaatggccc aaaaggcagc gggtttttta
     1201 atgattcttt tcctctcatg agcagaccaa taactacggc tccgcaagcg aatgaggcag
     1261 caaggaaaac cagtcgtggt aagaaggccc atgaatgcta ggcgccaagg gcggcgagaa
     1321 acttcacatc accatagcct aatccttctt tatgtcgcag tatgcgatac ccccagtaaa
     1381 tgacagcgaa tgtgccgtaa ccaacaattg ccccccataa cgcatcggct aaaccatcag
     1441 gatgacaaac ttgatagaac aaaagacctg accagagtaa cgggcacgta aatctgtcag
     1501 gtaacaagcc atgctttgca tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa
     1561 ttagaaaggg aagggtagtg tccataaaac atcctctgta attggagtat caccatcgaa
     1621 taactccgga tgctcagcaa cagagagagc gctgatttgt taacctgatt ccggtgaaaa
     1681 acgctatctt cacaacctta caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga
     1741 ttaatttcta cccgatgctt gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg
     1801 tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc taacaatgct
     1861 cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa tgggtctgag
     1921 gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga accaaagaat
     1981 ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt
     2041 cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa
     2101 agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga
     2161 aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa ccgttgatgc
     2221 tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg gttaatcagg
     2281 tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa agtgggtatg
     2341 acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga agttgaagca
     2401 aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat tcaggtgacc
     2461 accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt cgctaaagct
     2521 ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga agagttcact
     2581 gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga cgtaactggc
     2641 acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg tacccaggac
     2701 gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca gaaccagact
     2761 ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc
     2821 gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct ggttaaaggt
     2881 gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga
     2941 gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac
     3001 cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt atgcagctgg
     3061 tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa
     3121 accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga gcccgatctg
     3181 gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa
     3241 gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct
     3301 gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg cacagaaact
     3361 gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg aaaacctgtt
     3421 cctggctgcg cgcaacctgc agaaggttga cgtacgcgat gcaactggta tcgacccggt
     3481 tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc aagttgagga
     3541 gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct
     3601 gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac
     3661 gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc
     3721 gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt
     3781 agcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga cttcgttggc
     3841 ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa ccgacatctc
     3901 cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc aaaccttttg
     3961 ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc cgtatcacca
     4021 ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc aaacgcaaca
     4081 aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca
     4141 tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct aaaggcctga
     4201 aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggt aacaccctgc
     4261 cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa ccaggtaaag
     4321 gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgt gatggtgctt
     4381 atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt agaagcagac tgccgtgcaa
     4441 ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg
     4501 cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc
     4561 cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg tggggcgttc
     4621 agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc gtacgtcgcc
     4681 gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg tccttttatt
     4741 gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg
     4801 cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac catcgctgtc
     4861 cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg tcacaaactg
     4921 ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa agcgaagaag
     4981 aaataaggta ggaggaagag atggaaacta ttgctaaaca tcgccatgct cgttcttctg
     5041 ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg
     5101 atattttgac ctacaccaac aagaaagcgg ctgtacaggt caagaaagtt ctggaatctg
     5161 ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa gttacgaaaa
     5221 ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag
     5281 atcgcatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc tgagactctg
     5341 gagactagca atgggtcaga aagtacatcc taatggtatt cgcttgggta ttgtaaaacc
     5401 atggaactct acttggtttg cgaacaccaa agaattcgct gacaacctgg acagcgattt
     5461 taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc gtatcgttat
     5521 cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg gtatcgttat
     5581 cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt
     5641 tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa aactggttgc
     5701 tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc
     5761 tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta gcggccgtct
     5821 gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac
     5881 tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg gtgtaatcgg
     5941 cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg ttgaacaacc
     6001 ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc
     6061 gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cacaaaggcc gtaaccgcgg
     6121 tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg
     6181 tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg
     6241 tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa agccgctggc
     6301 agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga ttcagccggg
     6361 taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag cattcaagct
     6421 ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa
     6481 gcaaaagagc tgcgtgagaa gagcattgaa gagctgaaca ccgagctgct gaacctgctg
     6541 cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca gtctcacctg
     6601 ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga gaaggcgggt
     6661 gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga
     6721 aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacgga aaattcatca
     6781 agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc ggtgacgtgg
     6841 ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg gttcgcgttg
     6901 tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg gctcagaaat
     6961 gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg cgccctcgat
     7021 atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt agcggagcac
     7081 taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt
     7141 aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat
     7201 caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa
     7261 ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg tcattcgctt
     7321 cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg gtacgcgtat
     7381 ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta tctctctggc
     7441 accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta
     7501 tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg
     7561 gcaaggtcat tattgaaggt atcaacctgg ttaagaaaca tcagaagccg gttccggccc
     7621 tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa
     7681 tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa
     7741 aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta gtacgatggc
     7801 gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg agtttaacta
     7861 caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga
     7921 agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag caatctccgg
     7981 tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg
     8041 ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt tctttgagcg
     8101 cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt
     8161 cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga
     8221 ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta ctgcgaaatc
     8281 tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca agtaaggtag
     8341 ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt agctttagct
     8401 gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt gaacgcttcc
     8461 gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga ttccagcccg
     8521 tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc
     8581 gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa
     8641 aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat gagcatgcaa
     8701 gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg
     8761 gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt
     8821 tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact tactctgaag
     8881 tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc
     8941 atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt
     9001 tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa
     9061 attatctgct acgtagccta atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc
     9121 gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa
     9181 aacggcgagc tgactcgtac tctcaacgat gctgttgaag ttaaacatgc agataatacc
     9241 ctgaccttcg gtccgcgtga tggttacgca gacggttggg cacaggctgg taccgcgcgt
     9301 gccctgctga actcaatggt tatcggtgtt accgaaggct tcactaagaa gctgcagctg
     9361 gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc
     9421 tctcatcctg ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc gactcagact
     9481 gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc ggatctgcgc
     9541 gcctaccgtc gtcctgagcc ttacaaaggc aagggggttc gttacgccga cgaagtcgtg
     9601 cgtaccaaag aggctaagaa gaagtaaggt aacactatgg ataagaaatc tgctcgtatc
     9661 cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg cctggtggta
     9721 catcgtaccc cgcgtcacat ttacgcacag gtaattgcac cgaacggttc tgaagttctg
     9781 gtagctgctt ctactgtaga aaaagctatc gctgaacaac tgaagtacac cggtaacaaa
     9841 gacgcggctg cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa
     9901 gatgtatcct ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc actggcagat
     9961 gctgcccgtg aagctggcct tcagttctaa ggtagaggtg taagatggct cacatcgaaa
    10021 aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta
    10081 aaggtggtcg tattttctcc ttcacagctc tgactgtagt tggcgatggt aacggtcgcg
    10141 ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa
    10201 aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg cactctacaa caccctgtta
    10261 aaggtgttca cacgggttct cgcgtattca tgcagccggc ttccgaaggt accggtatca
    10321 tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg ggttcataac gttctggcta
    10381 aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc aactattgat ggcctggaaa
    10441 atatgaattc tccagaaatg gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg
    10501 ggaaataaac catggcaaag actattaaaa ttactcaaac ccgcagtgca atcggtcgtc
    10561 tgccgaaaca caaggcaacg ctgcttggct tgggtctgcg tcgtattggt cacaccgtag
    10621 agcgcgagga tactcctgct attcgcggta tgatcaacgc ggtttccttc atggttaaag
    10681 ttgaggagta agagatgcgt ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg
    10741 gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa aaccggtggt cgtggtcaca
    10801 aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc
    10861 ctctgtaccg tcgtctgccg aaattcggct tcacttctcg taaagcagcg attacagccg
    10921 aagttcgtct atctgacctg gctaaagtag aaggcggtgt tgtagacctg aacacgctga
    10981 aagcggctaa cattatcggt atccagatcg agttcgcgaa agtgatcctg gctggcgaag
    11041 taacgactcc ggtaactgtt cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg
    11101 aagctgctgg cggtaaaatc gaggaataag tagcagatgg ctaaacaacc gggattagat
    11161 tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca gactgctgtt tgttatcggt
    11221 gcgctgattg tgttccgtat tggctctttt attccgatcc ctggtattga tgccgctgta
    11281 cttgccaaac tgcttgagca acagcgaggc accatcattg agatgtttaa catgttctct
    11341 ggtggtgctc tcagccgtgc ttctatcttt gctctgggga tcatgccgta tatttcggcg
    11401 tcgatcatta tccagctgct gacggtggtt cacccaacgt tggcagaaat taagaaagaa
    11461 ggggagtctg gtcgtcgtaa gatcagccag tacacccgtt acggtactct ggtgctggca
    11521 atattccagt cgatcggtat tgctaccggt ctgccgaata tgcctggtat gcaaggcctg
    11581 gtgattaacc cgggctttgc attctacttc accgctgttg taagtctggt cacaggaacg
    11641 atgttcctga tgtggttggg cgaacagatc actgaacgag gtatcggcaa cggtatttca
    11701 atcattatct tcgccggtat tgtcgcggga ctcccgccag ccattgccca tactatcgag
    11761 caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt tggttgcagt attagtattt
    11821 gcagtgacgt tctttgttgt gtttgttgag cgtggtcaac gccgcattgt ggtaaactac
    11881 gcgaaacgtc agcaaggtcg tcgtgtctat gctgcacaga gcacacattt accgctgaaa
    11941 gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca gtattattct gttcccggcg
    12001 accatcgcgt catggttcgg gggcggtact ggttggaact ggctgacaac aatttcgctg
    12061 tatttgcagc ctgggcaacc gctttatgtg ttactctatg cgtctgcaat catcttcttc
    12121 tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag
    12181 tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa cggcgaagta tatcgataaa
    12241 gtaatgaccc gcctgacctt ggttggtgcg ctgtatatta cctttatctg cctgatcccg
    12301 gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg gtgggacctc actgcttatc
    12361 gttgttgtcg tgattatgga ctttatggct caagtgcaaa ctctgatgat gtccagtcag
    12421 tatgagtctg cattgaagaa ggcgaacctg aaaggctacg gccgataatt ggtcgcccga
    12481 gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc aagaaattat gccgtaactg
    12541 caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa
    12601 acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa agttgggttg agctggctag
    12661 attagccagc caatcttttg tatgtctgta cgtttccatt tgagtatcct gaaaacgggc
    12721 ttttcagcat ggaacgtaca tattaaatag taggagtgca tagtggcccg tatagcaggc
    12781 attaacattc ctgatcataa gcatgccgta atcgcattaa cttcgattta tggcgtcggc
    12841 aagacccgtt ctaaagccat cctggctgca gcgggtatcg ctgaagatgt taagatcagt
    12901 gagctgtctg aaggacaaat cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa
    12961 ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc tgatggatct tggttgctat
    13021 cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca
    13081 cgtacccgta agggtccgcg caaaccgatc aagaaataat cggggtgatt gaataatggc
    13141 aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca
    13201 tatccatgct tctttcaaca acaccatcgt gactatcact gatcgtcagg gtaacgcgtt
    13261 gggttgggca acagccggtg gttccggttt ccgtggttct cgcaaatcca ctccgtttgc
    13321 agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa gaatacggca tcaagaatct
    13381 ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct actattcgtg ctctgaacgc
    13441 cgcaggtttc cgcatcacta acattactga tgtgactccg atccctcata acggttgtcg
    13501 tccgccgaaa aaacgtcgcg tataacgcct cgttttccag gtttgttgga gaaagaaaat
    13561 ggcaagatat ttgggtccta agctcaagct gagccgtcgt gagggcaccg acttattcct
    13621 taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt gaacaagctc ctggccagca
    13681 cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt
    13741 tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac tactacaaag aagcagcacg
    13801 tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt
    13861 tgtataccgt atgggcttcg gtgccactcg tgcagaagca cgtcagctgg ttagccataa
    13921 agcaattatg gtaaacggtc gtgttgttaa catcgcttct tatcaggtta gtccgaatga
    13981 cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct
    14041 ggctgagcag cgtgaaaagc caacttggct ggaagttgat gctggcaaga tggaaggtac
    14101 gtttaagcgt aagccggagc gttctgatct gtctgcggac attaacgaac acctgatcgt
    14161 cgagctttac tccaagtaaa gcttagtacc aaagagagga cacaatgcag ggttctgtga
    14221 cagagtttct aaaaccgcgc ctggttgata tcgagcaagt gagttcgacg cacgccaagg
    14281 tgacccttga gcctttagag cgtggctttg gccatactct gggtaacgca ctgcgccgta
    14341 ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac
    14401 atgagtacag caccaaagaa ggcgttcagg aagatatcct ggaaatcctg ctcaacctga
    14461 aagggctggc ggtgagagtt cagggcaaag atgaagttat tcttaccttg aataaatctg
    14521 gcattggccc tgtgactgca gccgatatca cccacgacgg tgatgttgaa atcgtcaagc
    14581 cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag
    14641 ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat tcattcggaa gaagatgagc
    14701 gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc tgtggagcgt attgcctaca
    14761 atgttgaagc agcgcgtgta gaacagcgta ccgacctgga caagctggtc atcgaaatgg
    14821 aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg tgcggcaacc attctggctg
    14881 aacaactgga agctttcgtt gacttacgtg atgtacgtca gcctgaagtg aaagaagaga
    14941 aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga tctggaattg actgtccgct
    15001 ctgctaactg ccttaaagca gaagctatcc actatatcgg tgatctggta cagcgtaccg
    15061 aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc tcttactgag attaaagacg
    15121 tgctggcttc ccgtggactg tctctgggca tgcgcttgga aaactggcca ccggcaagca
    15181 tcgctgacga gtaaccggat cacaggttaa ggttttactg agaaggataa ggtcatgcgc
    15241 catcgtaaga gtggtcgtca actgaaccgc aacagcagcc atcgccaggc tatgttccgc
    15301 aatatggcag gttcactggt tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa
    15361 gagctgcgcc gcgtagttga gccgctgatt actcttgcca agactgatag cgttgctaat
    15421 cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg tggcaaaact gtttaacgaa
    15481 ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc gtattctgaa gtgtggcttc
    15541 cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg ttgatcgttc agagaaagca
    15601 gaagctgctg cagagtaatc tgaagcaatg taaaaaaacc cgccccggcg ggttttttta
    15661 tacccgtagt atccccactt atctacaata gctgtactct ttt
//
LOCUS       DAJTSF010000088        15343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000088 DAJTSF010000000
VERSION     DAJTSF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.088"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO335_003044"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO335_003044"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6003342.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO335_003045"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO335_003045"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6003343.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO335_003046"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO335_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR6003344.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="OO335_003047"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="OO335_003047"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6003345.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="OO335_003048"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="OO335_003048"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6003346.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="OO335_003049"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="OO335_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6003347.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="OO335_003050"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="OO335_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR6003348.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="OO335_003051"
     CDS             complement(8064..9206)
                     /locus_tag="OO335_003051"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR6003349.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="OO335_003052"
     CDS             complement(9215..10228)
                     /locus_tag="OO335_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR6003350.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO335_003053"
     CDS             complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO335_003053"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6003351.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO335_003054"
                     /gene_synonym="rfaI"
     CDS             complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO335_003054"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6003352.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHIFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO335_003055"
     CDS             complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO335_003055"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6003353.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12826..13950)
                     /locus_tag="OO335_003056"
     CDS             complement(12826..13950)
                     /locus_tag="OO335_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6003354.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OO335_003057"
     CDS             complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OO335_003057"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6003355.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4380 a         3444 c         3145 g         4374 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt
    10861 taaccctgca agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta
    10921 tgaaaacttt caacccattt atcgttttat tatatatcat aatgaattat tttaacctta
    10981 aatctttaga agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa
    11041 tgcttttgtt taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta
    11101 agtaaatctt ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc
    11161 ggataatcag cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg
    11221 tcatcattga ctggattatc aactttgtct tttaattcat agttaatact atatcgggta
    11281 ttatatttct ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga
    11341 tcaaggtggg tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac
    11401 gaaatgttat ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat
    11461 ccagaagcta actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct
    11521 ctttcagcta caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta
    11581 atactaccct tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag
    11641 aaataatctg caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt
    11701 aatgacttca gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt
    11761 gctaaatcag aaaaatataa tttgtcttta tcactgatat aatctgtgaa aatatgaaat
    11821 tcacaagaga tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa
    11881 agaaaatttc tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga
    11941 taatgaaaat tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt
    12001 ctgtatccaa aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt
    12061 tccctgattt ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga
    12121 atgtctttaa gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc
    12181 aggccaatat tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct
    12241 ggaacgcgcg tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct
    12301 tccttaccgg aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta
    12361 atgcccgcag catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt
    12421 acgcgaacat ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt
    12481 ggtgtcagat cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg
    12541 ccgaatgcca ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc
    12601 cattcgcggt ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct
    12661 ttcagggtcg tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc
    12721 agagtacggc gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca
    12781 aaaggatctt tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc
    12841 acccgttatg atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata
    12901 atgtcgcgca ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa
    12961 aatctcattc aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc
    13021 gacaatataa tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat
    13081 cgcttccagc agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc
    13141 cgccgccatt aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac
    13201 gccgcgtttt tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag
    13261 caatgtgttg tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc
    13321 acctttacgc gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc
    13381 attcttctta cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg
    13441 ataaatcccc ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa
    13501 atcggcgatt tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt
    13561 tgcccgctca aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt
    13621 ttcctgcgct actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg
    13681 cattttgttg aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca
    13741 ggcaaaatac tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac
    13801 ttcaaataca tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc
    13861 tcgggcggcg actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa
    13921 atatttatat aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt
    13981 aatttatcga cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt
    14041 cggtcacgct gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg
    14101 ttatttgacc agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc
    14161 gtattaactg cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca
    14221 tgatcgatta acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt
    14281 ggcgtctggc atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg
    14341 gacgtcagaa cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg
    14401 gcgttgtccc agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt
    14461 ccaacaccag cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc
    14521 atggttgtct gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt
    14581 tccaccacat ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca
    14641 gactgccgat gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt
    14701 atagcaacca tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat
    14761 accttgatga gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt
    14821 atgccgtaga gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa
    14881 agcagcacat cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta
    14941 gttaataaca tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta
    15001 tccacaagta actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct
    15061 accgccccag gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt
    15121 gtatcacttc tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca
    15181 ataaagaaat caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga
    15241 ttccttcctg cacgtacagc agaaatcaat gaatcgcata aaaatacaaa aaaagtatag
    15301 atgttcaaca agaattggcg ggtaaattgc tatttaagtc aaa
//
LOCUS       DAJTSF010000089        15226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000089 DAJTSF010000000
VERSION     DAJTSF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.089"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..2616
                     /gene="mdtB"
                     /locus_tag="OO335_003058"
                     /pseudo
     CDS             1..2616
                     /gene="mdtB"
                     /locus_tag="OO335_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            2617..5682
                     /gene="mdtC"
                     /locus_tag="OO335_003059"
     CDS             2617..5682
                     /gene="mdtC"
                     /locus_tag="OO335_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6003356.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            5683..7098
                     /locus_tag="OO335_003060"
     CDS             5683..7098
                     /locus_tag="OO335_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6003357.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            7095..8498
                     /gene="baeS"
                     /locus_tag="OO335_003061"
     CDS             7095..8498
                     /gene="baeS"
                     /locus_tag="OO335_003061"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6003358.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            8495..9217
                     /gene="baeR"
                     /locus_tag="OO335_003062"
     CDS             8495..9217
                     /gene="baeR"
                     /locus_tag="OO335_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6003359.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            9408..9740
                     /locus_tag="OO335_003063"
     CDS             9408..9740
                     /locus_tag="OO335_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR6003360.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            9887..11248
                     /gene="yegQ"
                     /locus_tag="OO335_003064"
     CDS             9887..11248
                     /gene="yegQ"
                     /locus_tag="OO335_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6003361.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(11590..11907)
                     /locus_tag="OO335_003065"
     CDS             complement(11590..11907)
                     /locus_tag="OO335_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003362.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            12313..13212
                     /gene="yegS"
                     /locus_tag="OO335_003066"
     CDS             12313..13212
                     /gene="yegS"
                     /locus_tag="OO335_003066"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR6003363.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(13294..14067)
                     /locus_tag="OO335_003067"
     CDS             complement(13294..14067)
                     /locus_tag="OO335_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6003364.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(14173..15213)
                     /gene="gatD"
                     /locus_tag="OO335_003068"
     CDS             complement(14173..15213)
                     /gene="gatD"
                     /locus_tag="OO335_003068"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6003365.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         3483 a         4051 c         3986 g         3706 t
ORIGIN      
        1 cgcgtcgcgc agaaaatctc gcagatttcc ggcgtcggcc tggtgacgct ttccggcggt
       61 cagcgtccgg ctgttcgcgt caaacttaac gctcaggcga ttgccgccct cggcctgacc
      121 agcgaaaccg tgcgcaccgc cattaccggc gctaacgtta actcggcaaa aggtagcctc
      181 gacggccctt cccgtgcggt cacgctttcc gcgaacgacc agatgcaatc cgccgaagag
      241 tatcgccagc taatcatcgc ctaccagaac ggcgcgccaa ttcgtctggg cgatgtcgca
      301 actgtagagc aaggtgcaga aaacagctgg ctcggcgcgt gggcgaacaa agaacaggcc
      361 attgtgatga atgttcagcg ccagcccggt gctaacatta tctccaccgc cgacagcatt
      421 cggcagatgc tgccacagct cactgagagt ctgccgaaat cggtgaaggt gacagtgctt
      481 tccgatcgca ccaccaatat ccgcgcatcc gtcgatgata ctcagtttga attgatgatg
      541 gctatcgcgc tggtagtcat gattatctac ctgtttttgc gcaatattcc ggcgaccatc
      601 attcccggtg ttgctgtacc gctgtcgtta atcggcactt tcgcggttat ggtgtttctc
      661 gatttttcaa tcaataacct gacactgatg gctttaacta tcgccaccgg attcgtggtc
      721 gatgacgcca tcgtggtgat cgaaaacatt tcccgctata tcgaaaaagg cgaaaaaccg
      781 ttggcggcgg cgctcaaggg cgcaggtgaa atcggcttta ccattatctc gctgaccttc
      841 tcactgattg cggtgttgat cccactgctg tttatgggcg atatcgtcgg gcgactgttc
      901 cgcgaatttg ctattaccct ggcggtagcg attttgatct cagcggtggt gtcgctgacc
      961 ctgacaccga tgatgtgcgc gcggatgctc agccaggagt cgttgcgtaa acagaacagc
     1021 ttctcccgtg cctcggaaaa aatgttcgac aggataatcg ccgcctatgg tcgtggactg
     1081 gcgaaagtgc tgaatcatcc gtggctgacc ttaagcgtgg cactcagcac gctgctgctt
     1141 agcgtgctgc tgtgggtgtt cattccgaaa ggtttcttcc cggtacagga caacggcatt
     1201 attcagggca ctttgcaggc accgcaatcc agctcctttg ccaatatggc ccagcgacaa
     1261 cgccaggtcg cggacgtgat tttgcaggat ccggcagtgc aaagcctgac ctcatttgtt
     1321 ggcgttgatg gcactaaccc gtcgctgaat agtgcacgtt tacaaatcaa cctcaaaccg
     1381 ttggatgaac gtgatgatcg ggtgcaaaaa gtcatcgccc gtctgcaaac ggcggtagat
     1441 aaagtgccgg gcgtcgatct cttcctgcaa ccaacgcagg acctgaccat cgatactcag
     1501 gtcagccgca cccagtacca gtttaccttg caggccacgt cactggatgc gctcagtacc
     1561 tgggtgccac agttgatgga aaaactccag caactgccac agctttctga tgtctccagc
     1621 gactggcagg acaaagggct ggtggcgtat gtcaatgttg atcgcgacag cgccagccgt
     1681 ctggggatca gcatggcgga cgtcgataac gccctgtaca acgcgtttgg tcagcgactg
     1741 atttccacta tttatactca ggctaaccag tatcgcgtgg tgctggagca caataccgaa
     1801 aataccccag gcctcgcggc gctggatacc attcgcctga ccagcagcga cggtggcgtg
     1861 gtgccgttaa gctcaattgc caaaattgag cagcgttttg cgccgctttc catcaaccat
     1921 ctggatcagt tcccggtaac aaccatctcc tttaacgtgc cggataacta ttcgctgggc
     1981 gatgcggtgc aggcgattat ggacaccgaa aagacgctga atctgccggt ggatatcacc
     2041 acgcaattct agggcagcac cctcgccttc cagtcggcgc tgggcagcac tgtctggctg
     2101 attgtcgcgg cggtggtggc gatgtatatc gtgctcggca ttctgtacga gagctttatt
     2161 cacccgatca ccattctctc gacgctaccc accgcagggg tcggcgcgct gctggcatta
     2221 atgattgccg gtagcgaact ggatgtgatt gcgattatcg gcattatttt gctgatcggt
     2281 attgtgaaga agaacgccat catgatgatc gacttcgcgc tggctgctga gcgcgagcaa
     2341 ggcatgtcgc cgcgcgatgc aatctaccag gcttgtctgt tgcgttttcg tccgatcctg
     2401 atgaccaccc tggcggctct gcttggcgcg ctgccgctga tgttgagtac cggggtcggc
     2461 gcggaactgc gtcgtccgtt aggtatcggc atggtcggcg gtctgattgt cagccaggtg
     2521 ctgacgctgt tcaccacgcc ggtgatttat ctgctgttcg accgcctggc attgtggacc
     2581 aaaagtcgct ttgctcgcca tgaagaggag gcgtaagtga agttttttgc cctcttcatt
     2641 taccgcccgg tggcgacgat tttactgtcg gtcgccatta ccctgtgcgg catactgggt
     2701 ttccgtatgc tgccggtcgc cccgctgccg caggtcgatt ttccggtgat tatggtcagc
     2761 gcctcgctgc ccggtgcgtc gccggagaca atggcgtctt ccgttgccac gccgctggag
     2821 cgctcacttg ggcgcattgc cggggttagt gaaatgacct ccagcagttc gctcggcagc
     2881 acgcgcatta ttttgcagtt tgattttgac cgggatatca acggcgcagc gcgtgatgtg
     2941 caggcggcga tcaacgctgc acaaagtttg ctgcccagcg ggatgcccag ccgcccgacc
     3001 tatcgcaaag cgaacccgtc ggatgcgcca attatgatcc tcacgctgac atccgatact
     3061 tattcgcagg gtgaactgta cgatttcgcc tcgacgcagc tggctccgac gatttcgcaa
     3121 atcgacggtg tcggtgatgt cgatgtcggt ggcagctcgc tgcccgccgt gcgcgtcggg
     3181 ctgaccccgc aggcgctgtt taatcagggc gtgtcgctgg acgacgtacg cactgccatc
     3241 agcaatgcca acgtgcgtaa accgcagggg gcgctggaag atggcactca ccgctggcag
     3301 atccagacca acgatgaact gaaaaccgcc gctgaatatc agccgctgat tattcactac
     3361 aacaacggcg gcgcggttcg tctgggcgat gtggcgacgg tgactgactc agtgcaggat
     3421 gtgcgcaacg ccgggatgac caacgccaaa ccggctattt tactgatgat ccgcaaactg
     3481 ccggaagcca atattatcca gacggtagac agcatccggg caaaattacc agagttgcag
     3541 gagaccattc cggcggcgat tgatctgcaa attgcccagg atcgctcccc caccattcgc
     3601 gcctcgctgg aagaagtcga gcaaacgctg attatctcgg tggcgctggt gattctggtg
     3661 gtctttttat tcctgcgctc gggtcgcgcc actattattc ccgccgttgc ggtgccggtt
     3721 tcgctgattg gtacgtttgc ggcgatgtac ctgtgcggat tcagtctcaa taatctttcg
     3781 ctgatggcgc tcaccattgc tactggtttc gtggtggatg acgccattgt ggtgctggaa
     3841 aacattgccc gtcatctgga agcggggatg aaaccactac aggccgcgct gcaaggtact
     3901 cgcgaagtcg gctttacggt gctgtcgatg agtctgtcac tggtggcggt gttcctgccg
     3961 ctgctgttga tgggcggatt gccgggccga ctgttacgcg agtttgccgt gacgctttct
     4021 gtcgccattg gtatttcgtt gctggtttcc ctgacgctaa cgccaatgat gtgcggctgg
     4081 atgctaaaag ccagcaagcc gcgtgagcaa aagcgactgc gtggttttgg ccgcatgttg
     4141 gtagccctgc aacaaggcta cggcaagtcg ctgaaatggg tgctcaatca tacccgtctg
     4201 gtgggcgtgg tgctgcttgg caccattgcg ctgaatatct cgatcccgaa aaccttcttc
     4261 ccggagcagg acaccggcgt actgatgggt gggatacagg cggatcagag tatttcgttt
     4321 caggcgatgc gcggcaaatt gcaggatttc atgaaaatta tccgtgacga tccggcagtg
     4381 gataatgtca ccggctttac cggcggttca cgagttaaca gtgggatgat gtttatcacc
     4441 ctcaagccac gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg tctgcgcgtg
     4501 aagctggcga aagaaccggg ggcgaatctg ttcctgatgg cggtacaaga tattcgcgtt
     4561 ggtgggcgtc agtcgaacgc cagctaccag tacacgttgt tatccgacga cctggcggca
     4621 ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cgttgccgga actggcggac
     4681 gtgaactccg atcagcagga taacggcgcg gagatgaatc tggtttacga ccgcgacacc
     4741 atggcacggc tgggaatcga cgtacaggcc gccaacagtc tgttaaataa cgccttcggt
     4801 cagcggcaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt gatggaagtg
     4861 gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat caataacgaa
     4921 ggcaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaatgc cccactatcg
     4981 gtgaatcatc agggattatc ggcggcctcg accatctcgt tcaacctgcc gaccggaaaa
     5041 tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg tgtgccttcg
     5101 acggtgcgcg gcagttttgc cggcacggcg caggttttcc aggagacgat gaactcgcag
     5161 gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat cctttacgag
     5221 agttacgtac atccgctgac gattctctcc accctgccct cggcgggcgt tggagcgctg
     5281 ttggcgctgg agctgttcaa tgccccgttc agcctaatcg ccctgatagg gatcatgcta
     5341 ttaatcggca tcgtgaagaa aaacgccatt atgatggtcg attttgcgct tgaagcccaa
     5401 cggcacggta acctgacgcc gcaggaagct attttccagg cctgtctgct gcgttttcgc
     5461 ccgattatga tgaccaccct ggcggcgctg tttggcgcgc tgccgctggt attgtcgggc
     5521 ggtgacggct cggagctgcg gcaacccctg gagatcacca ttgtcggcgg tctggtgatg
     5581 agccagctcc tgacgctgta taccacgccg gtggtgtatc tctttttcga ccgtctgcgg
     5641 ctgcgttttt cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga tcttcccgac
     5701 agcacccgct ggcaattatg gattgtggct ttcggcttct ttatgcagtc gctggacacc
     5761 accatcgtaa acaccgccct tccctcaatg gcgcaaagcc tcggggaaag tccgttgcat
     5821 atgcacatgg tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc cgccagcggc
     5881 tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt gctgtttact
     5941 ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct ggcacgcgcg
     6001 ttacagggcg ttggcggtgc gatgatggtg ccggtcggca ggttgacggt gatgaaaatc
     6061 gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgttacccgg tcaggtcggt
     6121 ccgctgctcg gtccggcgct cggcggtctg ctggtagaat acgcgtcgtg gcactggatc
     6181 tttttgatca acattccggt ggggattatc ggtgcgatcg ccacattgat gttaatgccg
     6241 aactacacca tgcagacgcg gcgctttgat ctttccggct ttttattgct ggcggttggc
     6301 atggcggtat taaccctggc gctggacggc agtaaaggta caggtttatc gccgctggcg
     6361 attgctggcc tggtcgcagt tggcgtggtg gcactggtgc tttatctgct gcacgccaga
     6421 aataacaacc gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt ttcgctgggc
     6481 ctggcgggga gctttgccgg acgtattggc agtggcatgt tgccctttat gacaccggtt
     6541 ttcctgcaaa ttggcctcgg tttctcgccg tttcatgccg gactgatgat gatcccgatg
     6601 gtgcttggca gcatgggaat gaagcgaatt gtggtacagg tggtgaatcg ctttggttat
     6661 cgtcgggtac tggtagcgac cacgctgggt ctgtcgctgg tcaccctgtt gtttatgact
     6721 accgctctgt tgggctggta ctacgttttg ccgttcgtcc tgtttttaca agggatggtc
     6781 aactcgaccc gtttctcctc catgaacacc ctgacgctga aagatctccc ggacaatctg
     6841 gcgagcagcg gaaacagcct gctgtcgatg attatgcaat tgtcgatgag tatcggcgtc
     6901 accatcgccg ggctgttgct ggggcttttt ggttcgcagc atatcagcgt cgacagcggc
     6961 accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtttatcat cgcccttccg
     7021 gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtagctat ttcgcggcga
     7081 aaaaggagcg cgcaatgaag ttctggcgac ccggtattac cggcaaactg tttctggcga
     7141 ttttcgccac ctgcattgtc ttgctgatca gtatgcactg ggcggtgcgt atcagttttg
     7201 agcgtggctt tattgattac atcaaacacg gcaatgaaca gcgattacag ctgttaagtg
     7261 atgcgcttgg cgagcagtat gcgcagcatg gtaactggcg cttcctgcgc aacaacgatc
     7321 gctttgtctt tcagatcctg cgttcatttg aacacgataa ttcggaagat aaacccggcc
     7381 cgggtatgcc gccgcacggc tggcgtaccc agttctgggt ggtcgatcaa aacaacaaag
     7441 tactggttgg tccgcgagcg ccgattccac ctgacggtac acggcgaccc attctggtca
     7501 acggtgcgga agtgggcgcg gtgattgcct cccctgttga gcggctgacc cgcaatactg
     7561 atatcaattt cgataaacaa cagcggcaaa ccagctggct gatcgtcgcc ctggcaacgt
     7621 tactcgcggc gctcgccacc tttctgctgg cgcgcggttt gctggcaccg gtaaaacgac
     7681 ttgtcgatgg cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta acgcccacca
     7741 gtgaagatga actgggcaaa ctggcgcaag acttcaacca gctcgccagc acgctggaga
     7801 aaaaccagca aatgcggcgc gattttatgg ccgatatttc tcacgaactg cgtacgccat
     7861 tagcggtgct gcgcggtgaa ctggaagcca ttcaggatgg cgtgcgcaaa ttcacgccgg
     7921 agacggtggc gtctttacag gcggaggtcg gtacactgac caaactggtt gacgatcttc
     7981 atcagttgtc gatgtctgat gaaggcgctc tcgcctatca aaaagcaccg gtagatttga
     8041 tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagc cgtggcctga
     8101 aactgcaatt ttccctgcca gacagtatca ccgtatttgg cgatcgcgac cgtttaatgc
     8161 agttattcaa taacttactg gaaaacagcc tgcgctacac tgacagcggc ggcagcctga
     8221 aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac agtgcgccag
     8281 gtgtcagtga cgatcagcta caaaaattgt ttgaacgttt ttatcgcacc gaaggttccc
     8341 gcaaccgcgc cagcggcggt tccgggctgg ggctggcgat ttgcctgaac attgttgaag
     8401 cccataatgg ccgcattatt gccgcccatt cgccttttgg cggggtaagc attacagtag
     8461 agttaccgct ggaacgggat ttacagagag aagtatgacc gagttaccaa tcgacgaaaa
     8521 cacaccgcgt attttgatcg tggaagatga accgaagctg gggcagttgc tcattgatta
     8581 tctgcgtgct gcgagctatg cgccgacgct tatcagccac ggcgatcagg tactggcgta
     8641 tgtgcgccag acaccaccgg atctgatcct gttggatctg atgctccctg gcaccgatgg
     8701 cctgacatta tgccgggaaa ttcgtcgttt ttccgatgtt ccgatcgtga tggtgacggc
     8761 aaaaatcgaa gagatcgacc gcctgctggg gctggagatt ggcgccgatg actacatctg
     8821 caagccgtac agcccacggg aagtggtagc gcgtgtcaaa accattttgc gccgttgtaa
     8881 accgcaacgc gaacttcagc aacaggatgc cgaaagcccg ttgattatcg acgaaggtcg
     8941 ttttcaggct tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat ttcgtctgct
     9001 taaaacgctc tctcacgaac caggaaaagt gttctcccgc gagcaattgc tcaatcatct
     9061 ttatgacgac taccgcgtag taaccgaccg caccattgac agccacatta aaaatctgcg
     9121 ccgcaagctg gaatctctcg acgccgaaca gtcatttatc cgcgccgttt acggcgttgg
     9181 ttaccgctgg gaagccgacg cctgccgcat cgtttagttt tagcgacatt actttgttag
     9241 ccggagatgg cgttcagacc cgatccggca acattattcc acgcatggtc agcaaactga
     9301 cattatctct ccgccctggc aggcatcaga aaatcgctca tactttaatc agtaaacagc
     9361 acctttagat gctgttttcg ataagcaatt tcaatcaagg agtcattatg gctggttggt
     9421 ttgaactcag taagagcagt gataatcagt tccggtttgt gctaaaagcg ggcaatggtg
     9481 agactatcct caccagcgag ctgtatacct caaaagcctc tgcggaaaag ggcatcgcgt
     9541 cggtgcgtag caacagcccg caagaagagc gctatgagaa aaaaacggca agcaacggca
     9601 aattctattt caatctgaaa gccgctaatc atcaaattat cggctccagc cagatgtacg
     9661 ccaccgcgca atctcgtgaa accggaattg cctccgttaa agccaatggc acaagccaga
     9721 cggtgaaaga caatacgtaa ttacgatgcc gggcgtgggt tgttggcgtc cggcgcttta
     9781 ccagatcccc cctacagcgc tacaatgccc gcccttaaag tgggggcctc ccctaaccgc
     9841 ttcatcaggt gaagcggatc tgacctgtca tcagaacgag agaattatgt ttaaaccgga
     9901 actcctttcc ccggcgggaa cgctgaaaaa tatgcgttac gctttcgctt atggcgcaga
     9961 tgctgtttat gcgggccagc cgcgttactc cctacgtgtg cgcaacaacg aattcaacca
    10021 cgaaaatctt cagctcggca tcaatgaagc ccacgcgctg gggaaaaagt tttatgtcgt
    10081 ggtcaacatt gcaccgcaca acgccaagct gaaaaccttt atccgtgacc tgaaaccggt
    10141 ggtggaaatg gggccggatg cgctgattat gtccgatcca gggctgatta tgctggtgcg
    10201 tgagcacttc cctgaaatgc cgatccacct ctcggtgcag gctaacgccg tgaactgggc
    10261 gacggtgaaa ttctggcagc aaatgggact gacccgcgtg atcctctctc gcgagctgtc
    10321 gctggaagag attgaagaga tccataatca ggtgccggat atggagatcg agatcttcgt
    10381 tcacggcgca ctgtgcatgg cctactccgg tcgctgcctg ctctctggct atatcaacaa
    10441 gcgcgacccg aaccagggca cctgcaccaa cgcctgccgc tgggagtaca acgtccagga
    10501 agggaaagaa gatgacgttg gcaacatcgt acacaagtac gagccgattc cggtgcaaaa
    10561 tgttgagccg acgctgggta tcggcgcgcc aaccgacaaa gtgtttatga tcgaagaagc
    10621 ccagcgtccg ggcgagtata tgaccgcgtt tgaagatgag cacggcactt acatcatgaa
    10681 ctcgaaagat ctgcgcgcca tcgcccatgt agaacgcctg accaaaatgg gcgtgcattc
    10741 gctgaaaatc gaaggtcgta ccaaatcttt ctactattgc gcacgcaccg cacaggttta
    10801 ccgcaaagct atcgatgacg ccgctgcggg aaaaccgttc gataccagcc tgctggaaac
    10861 tctggaaggt ctggcgcatc gtggctatac cgaaggtttc ctgcgtcgtc atactcacga
    10921 cgattatcag aactacgaat acggttattc agtttctgac cgccagcagt ttgttggtga
    10981 gtttaccggt gagcgcaagg gggacctcgc agcggtagcg gtgaaaaata aattctccgt
    11041 tggcgacagt cttgagctga tgacgccgca aggcaacatt aactttaccc ttgagcacat
    11101 ggaaaacgcc aaaggcgaag ctatgccggt cgcaccaggc gatggttata ctgtgtggct
    11161 cccggtcccg caggatcttg agctaaatta cgcgctgcta atgcgtaatt tctccgggga
    11221 aaccacgcgt aacccccacg gtaagtgatt aatttcgatt atttttcccg gatggaaaat
    11281 tcttagaaac cgatcacata cagctgcatt tattaaggtt atcatccgtt tcgctgaaaa
    11341 acataatcca taaaatgcta gctgtaccag gaaccacctc cttagcctgt gtaatctccc
    11401 ttacacgggc ttattttttg tacataagct gtaaagaagg attgatccct gataaccgtc
    11461 ttccattgac cacatcgata gaatccccct tcatagcaca atgcctttca cgtaacggta
    11521 tcgtgctcgc ccaggttccg gctacgcaca gccagaacgc gcatgtttga cgcttacaaa
    11581 aaaatattct cactctccac atttgaatgt cagacgagcg acgccatgta atcctgcacc
    11641 ttctgtcttc aggtcaacta tctgcatttt tttgccctga gtaacacaga aatgggctgc
    11701 atcatttttt actatatttt ctgcaccaga tattctgccc ctggctaaag aagcttcggc
    11761 ttcggtgtag tattggttat cgagtttacg ctgaatatta cttttatatg caagaccaaa
    11821 tttaccgata cttgtttcat cattatgcac agcacaacca gatataagaa aaatactaat
    11881 taatgatata gcagctatct ttttcatctc accttccccc attaaatacc aacgacactc
    11941 tctagtgttt aaatataata atggcatgat tattataatt gaataggatt ataataaatg
    12001 ttctgtacaa catttcctac ataagtagga attacggaca ttgaggccct tcagggtaac
    12061 tccatggggg ctttaatata ttatattgaa gatgccactg tttagttgaa tattaggtat
    12121 atgctctttt ttgaaattta tcggtggcag ccgttagtat tcgctgtccc cattgcaagc
    12181 tcctggtggt aaccactgaa tcctccatac ttgaactgac tttttatcct ccgactttca
    12241 tcctgttctg actccacctt ttgttttctg ctctacacta tctacagacc aatcataaag
    12301 gcacatacga tcatggcaga atttcccgcc agcttactga ttcttaatgg taaaagtact
    12361 gacaatctac ccttgcgcga agcaattatg ctgttgcgtg aggaaggaat gacgatccat
    12421 gtgcgggtca cctgggagaa aggtgatgcc gcacgatttg tagaggaggc ccggaagttg
    12481 ggcgtcgcaa cggtgattgc cggtggtggc gatggcacca ttaatgaagt ttctacggcg
    12541 ttgattcagt gtgaggggga tgacataccc gcgctgggaa ttttgccatt aggaaccgcc
    12601 aatgattttg ccaccagtgt agggattcct gaggcactgg ataaggcgct gaaactggca
    12661 attgccggta atgccattgc gatagatatg gcgcaggtca acaaacaaac ctgttttatt
    12721 aatatggcga caggcggatt tgggacgcgt attaccacag aaacgccgga aaaattaaaa
    12781 gccgcgctgg gtggcgtctc ttacatcatt catggcttaa tgcgcatgga tactctgcaa
    12841 ccggaccgtt gtgaaatccg cggtgaaaac tttcactggc aaggtgacgc cctggtcatt
    12901 ggtattggta acgggcgtca ggccggtggc ggtcaacaat tgtgcccgaa cgcgttaatt
    12961 aacgatggct tgctgcaact gcgcattttt accggcgatg aaattcttcc ggctctcgta
    13021 tcaaccttaa aatctgacga agataacccg aatattatcg aaggcgcttc gtcgtggttt
    13081 gatatacaag ccccacacga aatcactttt aatcttgatg gcgaaccgtt gagtgggcaa
    13141 aacttccata ttgaaatact tccggcggcg ttgcgttgtc gattaccacc agattgtcca
    13201 ttattgcgtt aatcaaacta ttctctcttg aaccagggag aatccgtaat gccggatgcg
    13261 gtgtaaacac cttatccggc atacagaaca atactacacc gtaattaatt ttacccgtgt
    13321 cttctgcaat gccaatttat ccacctctga cacagcatca tcggtaacaa taatgtcaat
    13381 tttttcgatt ggcagtacct gattaaaacc gcgacgatta aatttcgatg aatcgagaac
    13441 ggcaatcact ttatttgcgg cggtgaccat cgccccgcta atcgaataac cttcattaaa
    13501 ggtcgtaata ccattaaccg catcaatacc atcagcaccg acaaacatta aatcggcatt
    13561 aatatcctgc aatgaacgct cggcaatgga accatgcatc gagcgcgttt tatggcggac
    13621 cgtgccacca cagacgacca gagtaatgtc tttattttcg gaaagggcaa acgctgccgg
    13681 gagactgttg gtaatcaccg tgatattttt agcggtcatt aatccttcgg cgataagcat
    13741 tgtggtactt ccgctgtcga gaataacggt catcccttca tggatcattt tgactgccgc
    13801 ctgcgctatt ctgttttttg gcgcgctggc gagctgaaat cgctctttaa acccgacttc
    13861 ctgggtctcg gtttcactat taccggacat tattttcgcc gcaccgccat gaaagcgcgt
    13921 aaccacgcct ttttgttcga gaaagcgcaa atcggcacgg attgtcgctt ccgaggcagc
    13981 aaatactccc gccagatcct gaacaagcac ggttccctgt tcattcacta attggatgat
    14041 cttattcctt cgctcgaatg aattcatcgt catttaccca tattcaattg tggctagtgt
    14101 aaacgaaggg ggaccattgg tgaatgatct gttttgcaca ataccgggcc agcatcacgc
    14161 tggcccgcgg tttcatggaa tgagcaacac tttgccctgc atggcattac gagcgatgtc
    14221 acgcaccgcc tgggtgaagc tttcaaagct tccacggtga gcgattaatg gctccaggct
    14281 taacttacgt tctgtcagca accggctcgc cgtttcccac tcctgccccg gccaagggct
    14341 ggaatagttc atccaactgc cgataaccgt cagctcttta cgcaatattt tgccaaacgt
    14401 tgccgatgtt aaatgcagat cctgatgcaa cgtgcccacc agcgccagtt gggcatgagg
    14461 accggcaatc tctaccgcca gttcgacagt ttgcggtacg ccagccgtct cgaggataag
    14521 ctgattaaag cgcagttcgc gtaaaacgct ctgcatttgc ggcgcgctca tttcgctact
    14581 gttaaatgtt tgcatcgcac cgaaagattt tgccagtgcc agtttttctg agctaatgtc
    14641 gatcgccgtc acactctttg ctcccagcgc gacagcgcac tgaatagcca gcaggccaat
    14701 ggttccggca ccaataataa taacgttttt attctcacaa ccttgcgcta aatgaaaagc
    14761 atgcaggcca acggtaatcg gctcaataaa agccccatcc tcaataggca tatccgtggg
    14821 tagagcaaag acattttttc gcttaacgac aatatattca gcaaatccac catcacgccg
    14881 cgagccaata aaatcatatt ttgcgcactg ggaataaaac cctttcaaac actctggaca
    14941 agtaaaacag ggtaataacg gcacacaggc aaccgcatcg ccagggtgta aatcatcaac
    15001 accggatccc accgcatcaa tatagccgct aaattcatgg cctaacgtta ttggataata
    15061 atgtgcacca tttttaaata tcctgggtaa atcggaacca cataagcccg agctggcaat
    15121 ttttacccgc acctcatcct gatgtttaat ttcaggaatg acgctttttg caacgcgcac
    15181 gataccatca gtatcattca ctactgattt cataaaaact cctgat
//
LOCUS       DAJTSF010000090        15026 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000090 DAJTSF010000000
VERSION     DAJTSF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.090"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="OO335_003069"
     CDS             complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="OO335_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR6003366.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="OO335_003070"
     CDS             complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="OO335_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR6003367.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="OO335_003071"
     CDS             complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="OO335_003071"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR6003368.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="OO335_003072"
                     /pseudo
     CDS             complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="OO335_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="OO335_003073"
     CDS             complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="OO335_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR6003369.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="OO335_003074"
     CDS             complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="OO335_003074"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR6003370.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6041..7180)
                     /gene="wza"
                     /locus_tag="OO335_003075"
     CDS             complement(6041..7180)
                     /gene="wza"
                     /locus_tag="OO335_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR6003371.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7489..7638
                     /locus_tag="OO335_003076"
     CDS             7489..7638
                     /locus_tag="OO335_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003372.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7839..9422
                     /locus_tag="OO335_003077"
     CDS             7839..9422
                     /locus_tag="OO335_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6003373.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="OO335_003078"
     CDS             complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="OO335_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6003374.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="OO335_003079"
     CDS             complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="OO335_003079"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6003375.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12422..13063)
                     /gene="udk"
                     /locus_tag="OO335_003080"
     CDS             complement(12422..13063)
                     /gene="udk"
                     /locus_tag="OO335_003080"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6003376.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13381..13623
                     /locus_tag="OO335_003081"
                     /pseudo
     CDS             13381..13623
                     /locus_tag="OO335_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13622..14563
                     /gene="mdtA"
                     /locus_tag="OO335_003082"
                     /pseudo
     CDS             13622..14563
                     /gene="mdtA"
                     /locus_tag="OO335_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14563..>15026
                     /locus_tag="OO335_003083"
     CDS             14563..>15026
                     /locus_tag="OO335_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCR6003377.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTS"
BASE COUNT         3512 a         3987 c         3830 g         3697 t
ORIGIN      
        1 cctaaatcgc ccagacgggt ggaaatataa ggaaacgcga ttaccacacc aacccggaat
       61 acagccagag aaatcaatgc taacggtaat tttttcttaa tcgcctcttt attcagatat
      121 tgaaatgccc actccagcaa ataaaacagg ataaaggtca taacccctga aaacgatccg
      181 gataatatta tccctgcgag aatcagggca tctgttttag gcgttttgat accaaactgt
      241 ttgatgctga gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt
      301 gctgtcgtgc gcttgccgcc gaatttaatg aaattcagca catagctgtt gctgtaaatc
      361 agatatttcg aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc
      421 tccaccgccg ccagcgccac caccagtccg accaccagat aaaagaaacg taatatctta
      481 cgatggttgt gtggcgaaat tgttttaaaa cgaatactcc acaccatgcc aataatgatc
      541 acaatataga caaacagcat agttgaagtg acgtatttac cggcatccaa cgactgacca
      601 aacaggtagt taaacgccgt gagtcccgcg ccaatcccta aggcaatcat caatttttta
      661 atgctgatgc gttctaaaaa cagcagtaac aagacaggta aaaaagtgac gatggtgatg
      721 gggaaacttt cgccaagctg ggcgattttg acgttaacca gcaggtagat cagtggcagc
      781 agcaggtagc tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact
      841 gtaggcggcg cgactgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc
      901 gatttccggt ttgcttaact gtaccagttg cagcacctct tcttcactga cggttttacc
      961 accggatttt tgcagcactt cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc
     1021 aatcgatagc gcctcacaca aaatcagcgg gtagttatcg acacgagaac tgaacaccag
     1081 cgcatccatc tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt
     1141 aaccacgttg ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc
     1201 gcccagcgcc atcatctcac gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc
     1261 atgcgccacc actgcgattt tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc
     1321 cagaatcgct tcggttgcca tatcaatgcc attattgata atccggcaac gccctggccc
     1381 gtacagacta ttgaaagcgt cagccacatg ctggctgggg gaaataaact gacagcccag
     1441 cgccagcatc tcgcggaata actggcgttt gcccgccacc agctggtgcg cgcgatcaat
     1501 cttcaccggc ggatagttat ttaaggtcgg gcatttctgg cagcccgttt tccagccttc
     1561 gcaaccgtcg gtaaaggcgc agcgtccggt tacactccag tggtcgtgca gcgtccagac
     1621 cagggtgaca tccggtttat ggtttttcac tttttcgcaa aagcgcacca cgctgttaag
     1681 attcaaccag tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt
     1741 aatggtgcga tataactcat tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa
     1801 cgcaatgttc gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg
     1861 atggctgacg ctctctttgc cgcctttgcc gtagccgtag acaaaatgtg acgccagccc
     1921 ctgttgcagc gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag
     1981 tcgcacatta aattgcagaa tattcattta atcaccttca ctcgcgcttt ttctcccacc
     2041 accagcgcgt tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg
     2101 ttaccaagcg tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca
     2161 atgtgtggac atgccatgtt atcggcaccc cgattgccga tagtgacgcc gtggcgaatg
     2221 gtgaaatcat cccccgccac cacgttttta ttgatcacta cggcgtaacc gtgatggata
     2281 gtaaagcggc ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg
     2341 ataatgcgat acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg
     2401 cgccacaccg aacaaaaatg agcgacgcga taggcaagta ccatgcagca cgggcgtaaa
     2461 ctccagctgt tggcgcgcag atcttccagc atctttagcg cccccgaata ccatcagcca
     2521 gacgtttgcc gttacgcacc gacagcagcg tcaacaaggt gcgccaggtc atacgtttat
     2581 tgcggatctg gtagagggta aacagctgat attttttact ggcgcggtcg aatttgtctt
     2641 tgtgcttgcg gtaaaagtgg aagtatccgg agaatttttt cggtgacgag gtgatctgca
     2701 tttcgccgtg attgatatgc aggatctgcg tcgcctcttc cactttccac ggttcgccgt
     2761 actccaccac catccgcagg aatatgtcgt aatcctgtgc tgctttcagt tcggtatcga
     2821 acaaacactc tttgaaacgc catgcccagg taaagacctg attgccaatg atattacgtt
     2881 tatagaacag gcggcgtgag tacggcgatt tgggatacag tggtaagctc gccggttggg
     2941 aataaacttc tccctagcaa acgtagtcgt tagcgtacaa aaaggcgtgt gtgaccagtt
     3001 gctgtttatg ggcgaggaag acgctcaggc ggttgggcgt ccattcatca tcatcatcga
     3061 tcccggtgat gtattcccct tgcgccagca taatcgcctg gttacgcacc gcgcacgccc
     3121 cgctgttaat gtcgttgtgg atgtaagtga tacgcggatc gttgagggca gtgacgtact
     3181 gttgcagttg ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt
     3241 agtcctggcg caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggttg
     3301 gcatatagat tgagatcagc ggattgtctt tcataggttg ttctcccggc ttatgagctg
     3361 gtttgcttgc cggatgcggc gtgagcgcct tatccggcct acggggcggt gcgaatgcag
     3421 gcgtcgttat ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc
     3481 gctggcgcgg cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc
     3541 aaagcggctc agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat
     3601 taacgtggtt ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg
     3661 cgtatcaatc aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg
     3721 ttcgctcatt aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa
     3781 tttggcaata gaggtcggtt tagcagcggt agtaatatcg ccctgaccaa tcagaatctc
     3841 cgacagccca ttgacattat tggtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc
     3901 gcagtcgatc aacaacacgc gtttattggt ctggctgatc accgccgcca ggttggcgca
     3961 gacaaaggtt ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc
     4021 ctgcatcatc gcgaagtgca gactggtacg cagactacgg atggcttcaa tcgccagatc
     4081 ggttggattc cccactgcca gtagctggct ctgtttatag cgtttaaccc ctttgatggt
     4141 tttgacgcta tcgcgcgctt tctgccactc cgacagcggg atgctggcat agacgctgat
     4201 tccgtgttct tccagcactt gcgggctttc gatgccgcga ttaaacaacg agcgcagcag
     4261 cacacccacg atagagagca tcaggccaag gataatcgcg ccgaggataa tcagcccttt
     4321 cttcggtttt agcacgccag gctgggtgat tgccgggtca ataatgcgca catcgccgac
     4381 ggtgctggct tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg
     4441 ctgaccagac tcgacatcgc gggtcagacg gacaatttcc tgctggtttt tcggcatcgc
     4501 cgttacgcga ccgttaagtt tggctttctc ttcttccagc gcctgacgtt tctccagcag
     4561 cgtgcggtac gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt
     4621 cagttcgttc aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttcaag
     4681 cggcagatca acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc
     4741 gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc
     4801 cgccgatttg cgctcaatat tttgttcctg atagttacga gcgatgctgt taagaatgtc
     4861 gcggatctgt tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc
     4921 gttctccgtt accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt
     4981 ggtgacggta aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc
     5041 ttcttttttc agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag
     5101 ggtgtagttt ttgtcatcca gcacattaag cgtaaacacc tgatccgcca tctctttcgg
     5161 gcggttaaag gtagtcactt tcaccgtctc gttctgacgc cccatcaggc gatcccagcc
     5221 cgccccgaaa atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac
     5281 cgttttacca agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg
     5341 tttgttggct aatgccgagc cgatatcctg caccagcgaa ttgccgctgt tttgctcgat
     5401 ttgtaccagt gcgtcggcac tataaatcgg cgtggcgaag aaagtgtaaa ccacggcaca
     5461 gagggcaaat acgacggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac
     5521 caggcgacca atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt
     5581 ttctgtcatt cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt
     5641 tcaagtaatg tgtacaccgc tgcaaacgtt tcccggcttt tgtgatacgg atcggggatt
     5701 tcacattcgt tatcccagtg accaaacagc atcactttgc cgcgcatctc cggtgccatc
     5761 tcgcataagc gttcgatatg gcgcttatcc atggtcaaaa tcaggtcata gttgcgacac
     5821 aggcggcggc tgatttgtcg tgcacagtgg ccttccagag acagttgatg ttctgcggcg
     5881 acgctgatag cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc
     5941 actttcagtt ccgggtgata acgttgcagt aagcgttccg ccatcgggga acggcaaata
     6001 ttgccgacac agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc
     6061 tggctgtatc cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac
     6121 ggttccagcg ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga
     6181 attctgtcgc catcaccagc gacgtcgcat cggacatatc cagctggtag atattggcaa
     6241 tcttgccgtt acgcccgccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt
     6301 tggaggtggt catgtcgata ccttcggcat tggccagagc ttcagtcagg gtcatgccgc
     6361 taaagtccat tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat
     6421 catcattacg cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt
     6481 cgccgttttg catcagcgcc tgtaaagaaa tgcgttcttc acgaccattg tgtgttagca
     6541 ccacgttgcg ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa
     6601 tggtcagtgg tacgttggtg atcgcctgtt gaccggattt attcacctga cctgagatat
     6661 aggccttttg tgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg
     6721 tcgctaagcg cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgaa
     6781 ccttgccgat atacgggtaa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc
     6841 tggagctgcg gtactgacca gctggcgtgg tgagttccgg gtgatcccag acggtgacat
     6901 taagaacgtc ccccggcccg acgcgatact ggtaattcgc gatctcgctt tccagcgtca
     6961 tattggggcg cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg
     7021 gataaacatt caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt
     7081 tgcccatcgt cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg
     7141 acaccaataa tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc
     7201 agagtaatta tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt
     7261 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat
     7321 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
     7381 tagctaccaa tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga
     7441 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
     7501 tattggctcg ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
     7561 acaattcggc aattggctat ttttaagaat tatattaagt atcattcaat atggttttta
     7621 ggagtttctt taggttgaca atatttaata ttgcgtctcc atatgcgata tttcttaaat
     7681 aatgttttat tattaccgct tttaattcag ggataatgtg aggttattac cctcaaataa
     7741 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
     7801 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
     7861 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
     7921 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
     7981 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctagcgtca atctcctggc
     8041 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
     8101 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
     8161 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg
     8221 tggtgacgca aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg
     8281 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
     8341 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
     8401 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
     8461 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca
     8521 atcagttggc tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc
     8581 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
     8641 ccaaaaccgc gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac
     8701 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtaagcagc attatgacgt
     8761 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc
     8821 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
     8881 tcggtgttgt tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc
     8941 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttaccagccc
     9001 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg
     9061 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     9121 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
     9181 cgaatggtca tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa
     9241 aacgtgaata tcacaccatt gccgggctgt tgatggagta tttgcagcgt attccaaaac
     9301 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
     9361 gcgtgcagaa ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt
     9421 gatggtatcg gccattttac ccaatgctgc gtaacgcctt ataacttcag caatgaagcg
     9481 ttgtctgtgg taaacggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc
     9541 gtttcctgta ggctggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat
     9601 gttacctcgc gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg
     9661 cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt
     9721 tcaccggctt gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat
     9781 cttacatatt cagaatataa tagtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc
     9841 ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac
     9901 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
     9961 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg
    10021 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
    10081 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc
    10141 cagattcaac atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt
    10201 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac
    10261 gttatcgaag ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat
    10321 catctgctgg aagttcatcc cttccatacg tgtgtcggtc atctcaacat gcgcctgtcc
    10381 ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
    10441 tgacattttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc
    10501 aacgttctcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
    10561 gcccggcagt gaaacctttc caccgttaag cttgccctgc agttgagtaa tttccagcaa
    10621 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
    10681 gttactggcc tgcaacaaaa tatcagctgt aaagccttgc agtccctgat aggccggttc
    10741 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
    10801 ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg
    10861 cgggaattgc agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgtcc
    10921 gctcagcgta ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
    10981 ctcctgccac tgggactgga aactcccctg cccctgaata ccttgttttg gtaaatcggc
    11041 accctgcaac tgccagttaa tttgttcaat agccgctgtt aaatcatgcg gataatcaga
    11101 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
    11161 ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg
    11221 gattgtcacc tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag
    11281 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
    11341 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac
    11401 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
    11461 cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag
    11521 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
    11581 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
    11641 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaagagtat
    11701 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
    11761 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
    11821 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
    11881 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca
    11941 gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga
    12001 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gtgtcaccgc
    12061 cagcgccagc tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc
    12121 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
    12181 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
    12241 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
    12301 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcac atcactccct
    12361 taacgccaat tacgtcaagg gcataccaac ttatcactga acggtacaca atttatcaag
    12421 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
    12481 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
    12541 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
    12601 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
    12661 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga
    12721 tgaccttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt
    12781 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc
    12841 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
    12901 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
    12961 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
    13021 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
    13081 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
    13141 tccgtctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa
    13201 ttcccggtgg caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt
    13261 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
    13321 ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta
    13381 ataagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
    13441 agtttaggtt tagtctcgtt tatctttacc cttgtctcgc ttgagctttc gcagtttggc
    13501 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
    13561 gccgggcgca tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc
    13621 attccaggaa ggccagcagg tcaaagcagg cgatttactg gcagaaattg accccagcca
    13681 gttcaaagtt gcattagcac aaacccaggg ccaactggca aaagataaag ccacgcttgc
    13741 taacgcccgc cgtgatctgg cgcgttatca gcaactggca aaaaccaatc tcgtctcccg
    13801 tcaggagctg gatgcccaac aggcgctggt cagtgaaacc gaaggcacca ttaaggctga
    13861 tgaagcaagc gtcgccagcg cgcagttgca actcgactgg agccgcatca ccgcaccagt
    13921 cgatggtcgc gttggtctca agcaagttga tgttggtaac caaatctcca gtggtgatac
    13981 caccggaatt gtggtgatca cccagacgca tcctatcgat ttagtcttta ccctgccgga
    14041 aagcgatatc gctactgtag tgcaggcgca aaaagccgga aaaccgctga tggtagaagc
    14101 ctgggatcgc accaattcga agaaattaag tgaaggcacg ctgttaagcc tcgataacca
    14161 aatcgatgcc acaaccggta cgattaaagt gaaagcacgc tttaataatc aggatgatgc
    14221 gctgtttccc aatcagtttg ttaacgcgcg catgttagtc gacaccgaac aaaacgccgt
    14281 agtgatccca acagccgccc tgcaaatggg caatgaaggc cattttgtct gggtgctgaa
    14341 tagcgaaaac aaggtcagca aacatctggt gacgccgggc attcaggaca gtcagaaagt
    14401 ggtgatccgt gcaggtattt ctgcgggcga tcgcgtggtg acagacggca ttgatcgcct
    14461 gaccgaaggg gcgaaagtgg aagtggtgga agcccagagc gccaccactc cggaagagaa
    14521 agccaccagc cgcgaatacg cgaaaaaagg agcacgctcc tgatgcaggt gttacccccg
    14581 agcagcacag gcggcccgtc gcgcctgttt attatgcgtc ctgtcgccac cacgctgctg
    14641 atggtggcga tcttactcgc cgggattatc ggttatcgcg ccctgcccgt ttcggcgctg
    14701 ccggaagtgg actatccgac cattcaggtg gtcacgctct acccaggtgc cagcccggat
    14761 gtcatgacct ctgccgttac cgcgccgctc gaacgccagt tcgggcagat gtctggcctg
    14821 aaacagatgt cgtcgcaaag ttccggcggt gcgtcagtta tcactttgca gttccagcta
    14881 acattaccgc tcaatgtcgc cgagcaggaa gtgcaggccg cgattaacgc tgcgaccaac
    14941 ttgttgccga gcgatctgcc taacccgccg gtttacagca aagtgaaccc ggcagatccg
    15001 ccgatcatga cgctcgccgt cacctc
//
LOCUS       DAJTSF010000091        14976 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000091 DAJTSF010000000
VERSION     DAJTSF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.091"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(519..1460)
                     /gene="fabY"
                     /locus_tag="OO335_003084"
                     /gene_synonym="yiiD"
     CDS             complement(519..1460)
                     /gene="fabY"
                     /locus_tag="OO335_003084"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6003378.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1505..1942)
                     /gene="dtd"
                     /locus_tag="OO335_003085"
     CDS             complement(1505..1942)
                     /gene="dtd"
                     /locus_tag="OO335_003085"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6003379.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1939..2811)
                     /locus_tag="OO335_003086"
     CDS             complement(1939..2811)
                     /locus_tag="OO335_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR6003380.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2805..3404)
                     /gene="yihX"
                     /locus_tag="OO335_003087"
     CDS             complement(2805..3404)
                     /gene="yihX"
                     /locus_tag="OO335_003087"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6003381.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3503..4288)
                     /locus_tag="OO335_003088"
     CDS             complement(3503..4288)
                     /locus_tag="OO335_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6003382.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4322..5218)
                     /gene="yihV"
                     /locus_tag="OO335_003089"
     CDS             complement(4322..5218)
                     /gene="yihV"
                     /locus_tag="OO335_003089"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR6003383.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5386..6282
                     /gene="yihU"
                     /locus_tag="OO335_003090"
     CDS             5386..6282
                     /gene="yihU"
                     /locus_tag="OO335_003090"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR6003384.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6306..7184
                     /gene="yihT"
                     /locus_tag="OO335_003091"
     CDS             6306..7184
                     /gene="yihT"
                     /locus_tag="OO335_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR6003385.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7201..8442
                     /gene="yihS"
                     /locus_tag="OO335_003092"
     CDS             7201..8442
                     /gene="yihS"
                     /locus_tag="OO335_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR6003386.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8580..9482
                     /locus_tag="OO335_003093"
     CDS             8580..9482
                     /locus_tag="OO335_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR6003387.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9681..11717
                     /gene="yihQ"
                     /locus_tag="OO335_003094"
     CDS             9681..11717
                     /gene="yihQ"
                     /locus_tag="OO335_003094"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR6003388.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11763..13137
                     /locus_tag="OO335_003095"
                     /pseudo
     CDS             11763..13137
                     /locus_tag="OO335_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13180..14583
                     /locus_tag="OO335_003096"
     CDS             13180..14583
                     /locus_tag="OO335_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6003389.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14651..>14976
                     /gene="ompL"
                     /locus_tag="OO335_003097"
     CDS             14651..>14976
                     /gene="ompL"
                     /locus_tag="OO335_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR6003390.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYL"
BASE COUNT         3673 a         3854 c         3736 g         3713 t
ORIGIN      
        1 gatttaatag cgtttgcttc ggtgatttat tactcattag ttgtttttct aaattaacaa
       61 cagaataact ttcttgttta atgagattaa tattggcaac cattatagcc tccttcccac
      121 ggtttaagag caaacataca acataatgat tcataacata cagagcagta aatgaatgat
      181 actgacaatg tatgccagcc cattatcttt gcataatatt tgtgagctat agattttaaa
      241 aatatttatg aaaaaagtat ggcaccagag ggggttttga ctatcaaaaa tcgctcaaac
      301 ctgaactaat atgatgagtt aagcattatc tgcaataatt gccactcagc tctacaaata
      361 atatttaaat gctcctgaac agtgatgtag caagacacgt atggtgccaa cgaagaataa
      421 aaatttataa agttcttatt caatataacc ggaatagtca gggactgatg taaatgaccg
      481 gaatgaatga gagaggcagc accgcccctc tccataagct actcttcttc gttcccgccc
      541 tcttcatacg ggccaaatgg cttcgcgggc agaacgatat acgtgccttc aaacactgca
      601 cccggcgtct cgtcgccaaa gatttcgacc tgcatctgca cccgtgcttt tcgtccgcgc
      661 gccagacggt cgagatcgcc gcttaaggca ccaaggtcgg ctaccgcatg gggtttaccg
      721 ctaatcggtt tgctgtagcg gatatgcgca tccgccagaa taatcgttcc gccgaggtgg
      781 cgttcacgca gcatcagcca gataagcccc caaccggtga gcgtcgccag cgagaataaa
      841 ctcccggcaa acagcgtatg gtgcggattc tgattgccgg tttccggcat ggtggtgata
      901 aatttttgcc cggtatattg ttgaatacgc acgcccattt tttcactaag cgggatatgt
      961 tcgtaccacg cctgttgcag ctgcgcgcac cagtcgccgc gatgcaaaat atcatccaga
     1021 gtggcgacgg gcttaatcat caaaaaatgg cgaatcggcg tggtggttgg cgtggtgatt
     1081 tctccctgat taacaaaccc cagcttggcg aaaaactcca ccgcgtcttc acgggcgcta
     1141 caggtcacgc gcttaacgcc ttcctgacgc gccaccgact ccagggtcat cgccatcagc
     1201 gttcctaacc ctttgtcctg cacgtccggg tgaacgacca taaagcgaat ggacgcttca
     1261 ttgtcggcat taatatacaa tcggcctacc gccaccagat taccctgctc gtcgacgacc
     1321 atctggtgat gcgccatcgc atcccacgcg tcgcgttccg aaccttttgg ttgatgcagg
     1381 ggcttacgca acatttccca gcgaaagtga tagtaacgct ctaactcttc ttctgtttgt
     1441 ggaacctgaa ggtgatacat agcgatactc tctcttgttt cccgtgacaa ccctggaagc
     1501 tggctcatac ctgcaaccag aatgtcacgg ggccatcatt gaccagcgat acctgcatat
     1561 cggcagcgaa gcgtcctgtt tgcgtgttca tctcttgctg gcggcagcgt tcgacgaaat
     1621 agtcatataa cgcctctgcg cgatccggtg atgcgccttt ggagaaactt gggcgcatcc
     1681 cccgttcggt atctgcggcg agggtaaact gggaaaccac cagtacactg ccgcccgcct
     1741 gttgcacgtt gagattcatc ttgccttcgg catcgctaaa gatgcggtag ccgagcacac
     1801 gctcgcacag acggtttgct ttctgttcgt cgtcatcctt ttcgacaccc aataacacca
     1861 aaagtcccgc gccaatttcg cccgtcactt ctccctccac ggtgacgctg gcacgggtta
     1921 cgcgttgaat taatgcaatc atggttcgtc gtcttcttct tgttcagctg cttgttttag
     1981 tttgcggtat tccccgagag tgacagtaat ttccgcgcca agcaagacga tacaccatgt
     2041 ccagtagacc caaacaaaga gaatggggat caccgccagc acaccgtaaa tgagctgata
     2101 tgacgggaac atggtgatat aaagcgcgaa acctttcttt cctgcttcga acaggagtgc
     2161 ggcgacaaac gcgccgacaa tcgcgtcgcg gttaggtacg cggatggtag gaacaatgct
     2221 gtacagcaac cagaaggaga tccacgacaa cagcaacgga aaaatacgca gcacgttgtc
     2281 gatgacagta ttgagatcgc tcgcccagcg cagggagagc aaataggaac tgatcgccag
     2341 actggcccct gccagcagcg gccccagcgt taaaatcatc cagtacacgg cgaacgagta
     2401 aatttttggt cgcgctcgtt tactgcgcca gatggtattc aacgcgctat cgatggagta
     2461 catcaacaat aacgcggtga cgatcagccc gcacgcccca acggcggtca tcttgttgga
     2521 attagcaaca aattgttcga tataccgctg aataacatcg ccagtagcag gcagaaagtt
     2581 ggcaaaaata aagtgacgta actgaatgct gacgtcggaa aacatgggaa aagcggcgaa
     2641 aagcgcaaaa acaacggcaa ccagcggcac taatgagagc aacgacacat aggcaaggtt
     2701 acctgccagg gttgtcatgt tgtcctcatc aatacgttgc cagagtagtt ttagccaggc
     2761 ccatagtgga cgggtacggt gcctggcttt gtcctgaatg gtttttagca taacaccttc
     2821 gcgaaatagt ccgggatggt ggttttatct ttcaccagaa tactggtaat gcctagctga
     2881 ttagctcctt ctatattatc ggcgttatcg tcgaaaaaga ccgtatcgct gggtgaaaaa
     2941 ccttccgcct gcaaaacatg ctggtaaatt cgtgcttcag gtttgcgcat ccccagatct
     3001 tgcgacagat agatatggtc agcagcatca cgaatttccg ggtattcttc cggccagaag
     3061 gtggtatgca ggcggttggt attggaaagc accaccacgc gatgcccctg ctcacgcagt
     3121 ttatgcatga tggcgatcac ttccgggcgc agcgcaacaa acaccgcctg ccagccgtga
     3181 gagaactgct cgtagcttag cggtagagcc atctcatgac acagcgcctc tgcgaacgct
     3241 tcgtcgctaa tttccccacg ctcatgctga tgaaacgcct cccccatatg aaaactcttc
     3301 ttaagcgatg ccagcggaat acgcgttaaa tcgctccagg ctcccagcac acggttaaag
     3361 tcgatatcga caatcacatt acctaaatca aagatataga gcatttttgc ctcctttcgc
     3421 gccacaagaa attaactgta gcgggaaagg atgggtttgg ctatgtggca aggtatgaag
     3481 ccaggttaac ggtcacaaaa atctacgcgt cttcctgggg aagaatgatt tcaatttggc
     3541 tgtcttgtaa gcgtgatttt agcgcagcgg gcggcagctc gtcggtaaag agcgcagtga
     3601 cctgtgccac gttaccaatt tcaaccgccg ccgaagcatg atacttagtg tgatcggcga
     3661 ccagcagaat atttctcgcg tgcgccatca tcgttttcac cacgttagct tcgtttacat
     3721 caaactccat caacgcgcca tcgctctcaa tcgccccaac gcttgttacc agataatcag
     3781 cacgaaaatc agccacaaag gacgccgctg aagggccaat gatcccgcta ttatgagagc
     3841 gcaacgtacc gccgggcacc atcacttcaa agcgcgggtt gtggtaaaga atatgcgcca
     3901 cacgcaggct gttggtgatt atccgcaaat gattatggtt aagtaacgcc cgggcaacat
     3961 gctcaacagt cgtaccaatg gtgataaata ttgttgaacc atcaggaata tagtctgcca
     4021 ccgcttcggc aatcgctttt tttttctcgg tttgcgaaac ctcacgctgc tcgaacgccg
     4081 tattaacgac gctggaagcc cgacccgcgc caccgtgatg gcgcgtaatc aggccttgct
     4141 cgcttaattt acgaatatcc cggcggaccg tctgcgtgga gacatccagc agatttgcca
     4201 gctcatcaat attcatatac ccacgctcgg cgatcagcat gaggagttgg tcgtgccgcg
     4261 ggttaccggt tagttcggta aggctcatga aaattcctcg aaaaaccatc accctggcat
     4321 tttatacaaa aagtgacaaa aaagatcggg tttgatcaca gtcagggatc ccggctcgtc
     4381 caccgggacg tgtgcatttt aacgccgcta caccgctggc gaagcggact gactccgcta
     4441 aatccccact tgttgccagc gccaccgcca aagcgccgtg aaaaacatct cccgcacctg
     4501 tggtatctac cacatcaact ttgaaggccg gttgatgctg acgcccacca ttttccagcc
     4561 agtcgcagcc tgcgctaccc tgggtcacat agacatgacc atttgtgagc gtttgtgcct
     4621 gttttagcgc actggccatc tctttcacgc ccgttaagcg cgccagaccc ggttctgaaa
     4681 aggccgcgtg atcgcttaat gccaccagct cactgatatc ctgcggcgta atgtccccgt
     4741 ccagaacggt catcacaccc gcctgacggg ccagggtgaa ggctttttta gcgccgtcgt
     4801 gccagcgtac atctgccagc acaacatccc actgagagaa atcaatttcc tccaaccact
     4861 ctgcgtcagg cagcagatcc gggctggggt agttaatgat tatccgctcg cctttggtat
     4921 ccaccatgat ggcggattgc gaagatttcg cctggttata ccgtttggtg taacgggtgt
     4981 taacgcccca ggattccagc tctgccagca ggctgttgcc cgcgtcgtca tcacctacgc
     5041 gaccaataaa atcgacctgc gcccccagcc ttgccgccgc aaccgctgcc gtcgccgctg
     5101 gcccgccacc aacttccgta taatttctcg ccacgtattt accgctctcc gtcggtaacc
     5161 cttccacgta atagatgcga tccatcacgg ttatacctac acaagcaaca cgaatcatgg
     5221 tcattcctta aacattttgg gatgcgttca ttttaatttc accaattgtt taaaaagtga
     5281 cggctgtcaa ttttttgacg ataaattaca aattcgatca aaaacagaca agcaaagcat
     5341 gtcataaatg acaaaaagtg acatcactgt attcaggaga gggttatggc agcaatcgcg
     5401 tttatcggtt taggacaaat gggttcgcca atggcgagca atttattgca gcaagggcat
     5461 caacttcgcg tctttgatgt gaacgccgaa gctgtgcggc atctggtaga caaaggtgcg
     5521 actcccgccg ccaacccggc gcaggccgct aaagatgccg aatttatcat taccatgttg
     5581 ccgaatggcg atctggtgcg caacgtgttg ttcggtgaaa acggcgtttg cgaaggctta
     5641 tctaccgatg cgctggtcat tgatatgtcc accatccatc cgctgcaaac cgataaattg
     5701 attgccgata tgcaagccaa aggcttcagc atgatggatg ttccggtagg ccgtacttct
     5761 gcaaatgcca ttaccggtac tctgttactg ctggctggcg gcaccgctga acaagttgaa
     5821 cgtgccacac cgatcctgat ggcgatgggc agtgagttga tcaacgctgg cggtccgggc
     5881 atggggatcc gcgttaagct catcaacaac tacatgagca tcgcgctcaa tgcgctttcg
     5941 gcagaagccg ccgttttgtg cgaagccctg aatcttccct tcgatgttgc cgtcaaagtg
     6001 atgagcggta ccgccgccgg taaaggtcac ttcaccactt cctggccaaa caaagtcctc
     6061 agcggagatc tttctcccgc cttcatgatc gatcttgccc ataaggatct tggcatcgcc
     6121 cttgatgtcg ccaaccagct gcatgtgcca atgccgctgg gggccgcctc acgggaggtt
     6181 tatagccagg cgcgcgcagc gggtcgcggt cgccaggact ggtccgccat tctggaacag
     6241 gtccgtgtca gtgccgggat gactgccaaa gtaaaaatgt aacgactgga taaaggaata
     6301 aatgaatgaa taagtacacc atcaacgaca ttacgcgcgc atcgggcggt tttgccatgc
     6361 tggcggtcga tcagcgcgaa gccatgcgca tgatgtttgc cgcggctggg gcacccgctc
     6421 cggtagccga tagcgtttta actgatttca aagttaacgc tgcaaaggcc ctctcgcctt
     6481 atgcctcggc gattctggta gatcaacaat tctgctaccg ccaggtggtt gagcaaaacg
     6541 cgattgccaa aagttgcgcc atgattgtcg ccgccgatga gttcattcct ggcaacggta
     6601 ttccggtcga tagcgtggtt attgaccgca aaatcaatcc gctacagatc aaacaagacg
     6661 gtggcaaagc cttaaaactg ctggtgctgt ggcgtagtga tgaagatgcg cagcaacgtc
     6721 tggatatggt gaaagagttc aacgaactgt gccactcaca cggtctggta agcatcattg
     6781 agccagtcgt ccgcccaccg cgtcgtggcg ataaattcga tcgcgaacaa gcgatcatcg
     6841 atgccgccaa agagctgggc gacagtggcg ctgacctcta caaagttgaa atgccgcttt
     6901 atggcaaagg tccgcaacaa gagcttctct gtgcttcaca acgtctgaat gaccatatca
     6961 atatgccatg ggtgatcctc tcttccagtg tcgacgaaaa actgttcccg cgtgccgtac
     7021 gcgtggcaat gacggcgggc gcatcgggat tcctggcagg ccgtgcagtc tgggcatcgg
     7081 tcgtcggttt accagacaac gagctgatgc tccgtgacgt ttgcgcaccg aaattacaac
     7141 aacttggcga tatcgtcgac gaaatgatgg ctaaacgccg ctaagatgag gataaaagga
     7201 atgaaatggt ttaacaccct aagccacaac cgttggctgg aacaggaaac cgaccgcatc
     7261 tttgattttg gtaaaaattc cgtagtgccg actggttttg gctggttagg caataaaggg
     7321 caaatcaaag aagagatggg cacccatctg tggatcacag ctcgtatgtt gcacgtttat
     7381 tccgttgctg cggcgatggg tcgacctggc gcttacgcgt tggttgatca cggtattaaa
     7441 gccatgaacg gcgcactgcg tgataaaaaa tatggcggct ggtatgcctg cgtgaatgac
     7501 gagggcgtgg tggatgcctc caaacagggc tatcaacact tctttgctct gctgggtgcc
     7561 gccagcgccg tcacaacggg tcacccggaa gcgcgcaagc tgctcgatta caccattgag
     7621 attatcgaga aatatttctg gagcgaagaa gagcagatgt gcctggagtc ctgggacgaa
     7681 gccttcagca aaaccgaaga gtaccgcggc ggcaatgcca atatgcacgc ggtggaagct
     7741 ttcttgattg tttatgacgt cactcacgac aaaaaatggc tggatcgcgc gattcgcgtg
     7801 gcttccgtga ttatccacga cgtcgccaga aataatcatt atcgcgttaa cgaacacttc
     7861 gatacccagt ggaatccgct gccggattac aacaaagata acccggcgca tcgcttccgc
     7921 gcgttcggag gtacaccagg ccactggatc gaatggggcc gtttaatgct gcacatccat
     7981 gctgccctgg aagcccgttg cgaacaacca ccagcatggc tgctagaaga tgccaaaggt
     8041 ctgtttaacg ccaccgtgcg cgatgcctgg gcacccgatg gagcggacgg gattgtttat
     8101 accgttgact gggaaggaaa accggtggtc cgcgaacgtg tacgttggcc tatcgtcgaa
     8161 gcaatgggta cggtctacgc gctctacacc gtcactggcg atcgccagta tgaaacctgg
     8221 tatcaaacat ggtgggagta ctgcattaag tacctgatgg actatgaaaa tggttcctgg
     8281 tggcaggagc tggatgcgga caataaggtc atcaccaaag tctgggacgg caaacaggat
     8341 atttatcacc tgctgcattg cctggtgatc ccgcgtatcc cgttagcgcc cggcatggct
     8401 ccagcggttg cagcgggtct gctggatatt aatgcgaaat aactgatctg aaaatagggt
     8461 gcgggttttc ccctcaccct aaccctctcc ccaaaggggc gaggggaccg tccggtgcag
     8521 tttttgggtt tgacatcagc ctcacgggag gctgtatgtc gttaattaag gtgccttgta
     8581 tgcaaatcac caatatgcat tgcagtggac agaccgtttc acttgccgcg ggggattatc
     8641 acgcgaccat tgtgaccgtc ggtgctggtc tggctgaatt aacctttcag ggttgtcatt
     8701 tagtcatccc gcacaaaccc gaagagatgc cgctggcgca tctgggtaaa gtgcttattc
     8761 cgtggccgaa ccggatcgcc aacggctgtt accgttatca ggggcaggaa tatcagttac
     8821 ccattaatga acatagttca aaagccgcta ttcacgggct gcttgcctgg cgagactggc
     8881 aaattagtga attaaccacc accagcgtta ccctgacagc ttttctgccg cccagttacg
     8941 gctatccgtt tatgctggct tcacaagttg tttattcgct caacgcacac acgggtcttt
     9001 ccgttgagat cgcaagccag aatatcggca ctgtcgcagc gccttatggc gtgggtattc
     9061 atccttattt aacctgcaac ttaacatctg ttgatgaata tctgttccag cttccggcaa
     9121 accaggttta tgccgtcgat gaacacgtaa atccgacaac gcttcaccac gttgatgagc
     9181 tggatttaaa tttcacgcag gcgaaaaaaa tagccgcgac aaaaatcgac cacaccttta
     9241 aaaccgctaa tgatttgtgg gaaatgacca ttacccatcc acagcaggcg ctttcggtaa
     9301 gcctttgttc cgatcaaccc tgggtgcagg tttatagcgg tgaaaaatta cagcgtcagg
     9361 gactggcagt tgaacctatg agttgcccgc ctaacgcctt taattcaggt atcgacttat
     9421 tattactgga accaggaaaa ccgcaccgcc tgttttttaa tatttacgga cagagaaaat
     9481 aacttgtatt gattagcagt caaaatcatt gaagaacatt tcactcgcta aaggcaacat
     9541 tatgtatctt caatttatta agttaaaaac agctcagctt ctgtaaagtt tttatatatc
     9601 agttttatga gaatcatttt acataagcct gtaataacag gaaggcaaca atctcgttct
     9661 caactctatg gagattagtt atggatacgc cacgtccaca gttattagat tttcaatttc
     9721 atcagaataa cgacagtttt accctacatt ttcaacaacg tcttatttta acccataaca
     9781 aagataatcc ttgtttatgg attggctcag gtatagcgga tatcgatatg ttccgcggta
     9841 atttcagcat taaagataaa ctacaggaga aaattgcgct taccgacgcc atcgtcagcc
     9901 agtcaccgga tggttggtta attcatttca gccgtggttc tgacattagc gccacgctga
     9961 atatctctgc cgacgatcag ggccgtttat tgctggaact acaaaacgac aaccttaacc
    10021 acaaccgtat ctggctgcgc cttgccgctc aaccagagga ccatatctac ggctgcggcg
    10081 aacagttttc ctacttcgat ctgcgcggca aaccgttccc gctatggacc agtgaacaag
    10141 gcgttggtcg caacaaacaa acctatgtca cctggcaggc cgactgcaaa gaaaatgcgg
    10201 gcggcgacta ttactggact ttcttcccac agcctacgtt tgtcagcacg cagaagtatt
    10261 actgccatgt tgataacagt tgctacatga acttcgactt tagcgccccg gaataccatg
    10321 aactggcgct gtgggaagac aaagctacgc tacgttttga atgtgctgac acatacatct
    10381 ccctgctgga aaaattaact gccctgctgg gacgccagcc agaactgccc gactggattt
    10441 atgacggggt aacgctcggc attcagggcg ggacggaagt gtgccagaag aaactggaca
    10501 ccatgcgtaa cgcgggcgtg aaggttaacg gcatctgggc gcaggactgg tctgggatcc
    10561 gtataacctc ttttggcaag cgcgtgatgt ggaactggaa gtggaacagc gaaaactacc
    10621 cgcaactgga ttcacgcatt aagcagtggt atcaggaggg cgtgcagttc ctggcctata
    10681 tcaacccgta tgttgccagc gataaagatc tctgcgaaga agcggcacaa cacggctatc
    10741 tagcaaaaga tgcctctggc ggtgactatc tggtggagtt tggcgagttt tacggcggcg
    10801 ttgtcgatct cactaatcca gaagcctacg cctggttcaa ggaagtgatc aaaaagaaca
    10861 tgattgaact cggctgcggc ggctggatgg ctgacttcgg cgagtatctg cccaccgaca
    10921 cgtacctgca taacggcgtc agtgccgaaa ttatgcataa cgcctggcct gcgctgtggg
    10981 cgaagtgtaa ctacgaagcc cttgaagaaa cgggcaagct cggcgagatc ctcttcttta
    11041 tgcgcgccgg ttctaccggt agccagaaat actccaccat gatgtgggcg ggcgaccaga
    11101 acgtggactg gagtctcgac gatggcctgg cgtccgtagt cccggcggcg ctgtcgctgg
    11161 caatgaccgg acatggtctg catcacagcg acattggcgg ttacaccacc ctgtttgaga
    11221 tgaagcgcag caaagagctg ctgctgcgct ggtgcgattt cagcgccttc acgccgatga
    11281 tgcgcaccca cgaaggtaac cgtcctggcg acaactggca gtttgacggc gatgcggaaa
    11341 ctatcgccca ttttgcccgt atgactaccg ttttcaccac cctgaaacct tatctgaaag
    11401 aggccgtcgc gctgaatgcg aagtccggtc tgccggtcat gcgcccgctg ttcctgcatt
    11461 acgaagacga tgcacatacc tattccctga aatatcagta cctgttaggc cgcgacattc
    11521 tggtcgctcc ggtacacgaa gaaggtcgca gcgactggac gctctatctg ccggaggata
    11581 actgggtaca tgcctggacg ggtgaagcgt tccggggcgg ggaagttacc gttaatgcgc
    11641 ccatcggcaa gccgccggtc ttttatcgcg ccgatagcga atgggcggca ctgttcgcgt
    11701 cgttaaacag catctaatca catccgcccg tggggaaact catgggcaaa gggagaacat
    11761 cgatgagtca catcacaacg gaagatccag caactttacg cctgcccttt aaagagaaac
    11821 tctcttacgg tattggcgac ctggcctcta acatcctgct ggatatcggt acgctttatc
    11881 ttttgaagtt ttataccgac gttctggggc tgccaggcac ctatggcggc attatctttt
    11941 tgatttcaaa attctttact gcgtttaccg atatgggtac cggcattatg ttggattccc
    12001 gacgcaagat cggtccaaaa ggtaagttcc gtccttttat tctgtatgcg tcattcccgg
    12061 tcaccttact ggcgatcgcc aactttgtcg gcacaccgtt tgatgtcacc ggtaaaacgg
    12121 tgatggccac tattctgttt atgctttacg gactgttttt cagcatgatg aactgctcct
    12181 acggcgcaat ggtcccggca atcaccaaaa accccaacga acgcgcctca ctggcggcat
    12241 ggcgtcaggg cggcgctacg ctgggcctgc tgctgtgcac ggtgggattc gtgccagtta
    12301 tgaatcttat cgaaggtaat cagaaacttg gctatatctt cgccgccacg ctgttttcac
    12361 tgttcggcct gctgtttatg tggatctgct actcaggcgt gaaagagcgt tatgtcgaaa
    12421 cccaaccaac caatccggcg caaaagcctg gcctgttgca atctttccgc gcaattgccg
    12481 gtaaccgccc actgttcatt ctgtgtattg ccaacctctg caccttaggg gcgtttaacg
    12541 tcaagctcgc cattcaggtc tattacaccc agtacgtact taacgatccc atcctgttgt
    12601 cgtatatggg atttttcagc atgggctgta ttttcatcgg cgtgttcctg atgcctggcg
    12661 cagtcagacg ttttggtaag aagaaggtct atatcggcgg cctgctgatt tgggtgctgg
    12721 gcgatctgct caactatttc ttcggcggcg gctcggtcag ctttgtggcg ttctcctgcc
    12781 tggcgttctt cggctcagcg cctgaactgg gcgctggttt ccgacaccgt cgagtacggc
    12841 gagtggcgca ccggcgtgcg ttcggaaggc acggtctaca ccggcttcac cttctttcgc
    12901 aaagtgtctc aggcgctggc tggtttcttc cccggctgga tgctgacgca aattggctat
    12961 gtgccgaacg tcgcacaggc tgaccacacc attgaagggt tacgccagtt gatcttcatc
    13021 tacccaagcg cactggcggt agtcaccatt gtggcgatgg gttgcttcta cagcctgaac
    13081 gagaagatgt atgtccgcat tgtggaagag atagaagccc gtaaacgcac ggcgtaataa
    13141 taataaacga cgccctgcgg ggcgttataa ggagtgatta tgtctgacca taatccactg
    13201 acattaaaac tgaatctgcg ggaaaaaatc gcctatggta tgggcgacgt cggttcgaat
    13261 ttaatgctct gcatcggtac tctgtatctc ctcaaacttt ataccgatga attagggatg
    13321 cctgcttact atggcgggat tatctttctg gtcgcgaagt tttttaccgc gtttaccgat
    13381 atgctcaccg gatttttact cgactcgcgg aaaaatattg ggcctaaagg caaatttcgt
    13441 ccctttattt tatatgcagc agttccggcg gcgttaattg caacgctcca gtttatcgcc
    13501 accacctttt gtttgccggt taaaacgacg attgccacca cgctgtttat gatgtttgga
    13561 ctttcataca gtctgatgaa ctgctcgtat ggtgcgatga tcccggcaat taccaaaaac
    13621 ccgaatgagc gcgcgcagct cgcggcttac cgccagggtg gtgcaaccat agggctattg
    13681 atttgtaccg tggcgtttat tccgttgcag tcgctttttt ctgactcaac cgtcggttat
    13741 gcctgtgcgg cacttatgtt ctccattggc ggctttattt ttatgatgct gtgctacaga
    13801 ggcgtcaaag agcattatgt ggacacagcg ccaaccggac ataaagccag tattctcaaa
    13861 tctttttgcg cgatatttcg taatccacca ttgctggttt tatgcattgc taacctgtgt
    13921 actctggcgg catttaatat caaactggcg attcaggtct attacaccca gtatgtgctg
    13981 aatgatatta atttattgtc gtggatggga ttcttcagta tgggatgcat cctcgtcggc
    14041 gtattactgg tgccagtaac tgtaaaatgt tttggtaaaa aacaggttta tttagctggc
    14101 atggtgctat gggcggtggg tgatatactg aattatttct ggggaagtaa ctctttcact
    14161 ttcgtcatgt tctcttgtgt cgcctttttt ggcacggcct ttgtaaacag cctgaactgg
    14221 gcactggtac cagataccgt agattacggt gaatggaaaa ccggtattcg tgccgaaggt
    14281 tctgtttata ccggttatac cttctttcgt aaaatttctg ccgcacttgc tggcttcttg
    14341 ccaggcatta tgctgacgca aattggttat gttcccaaca tcgcgcaaag cgatgcgaca
    14401 ttacagggtt tgcgtcagct catttttatc tggccttgtg cactggcaat tattgctgca
    14461 ttgacgatgg gattctttta cacactcaat gaaaaacggt ttgcattgat tattgaggaa
    14521 atcaaccaac gcaaaaataa agagatggcg acagaaaaaa aaacggcttc cgtaacgtta
    14581 taaatgtcaa tagccgctat ttccatcctg gtgggtggcg gcctccctac gtttaaaaaa
    14641 tggacttatt atgaaaaaga ttaatgcaat aattttatta tcttctctga cctctgcctc
    14701 ggtatttgct ggcgcatacg tcgaaaacag ggaggcatat aatcttgcct ccgatcaggg
    14761 cgaggttatg ctacgtgtgg gttataactt cgatatgggc gcgggtatta tgttaaccaa
    14821 tacctacacc tttcagcgag aagatgaact aaaacatggc tataatgaaa ttgaaggttg
    14881 gtatccttta ttcaaaccaa ccgataaatt aaccatccag cccggtggct taattaatga
    14941 taagagtatc agttcgggtg gtgcagtgta tctgga
//
LOCUS       DAJTSF010000092        14479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000092 DAJTSF010000000
VERSION     DAJTSF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.092"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(113..379)
                     /locus_tag="OO335_003098"
     CDS             complement(113..379)
                     /locus_tag="OO335_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6003391.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="OO335_003099"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="OO335_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6003392.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="OO335_003100"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="OO335_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="OO335_003101"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="OO335_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6003393.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="OO335_003102"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="OO335_003102"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6003394.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OO335_003103"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OO335_003103"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6003395.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OO335_003104"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OO335_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6003396.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="OO335_003105"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="OO335_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OO335_003106"
                     /pseudo
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OO335_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12641..14095)
                     /locus_tag="OO335_003107"
     CDS             complement(12641..14095)
                     /locus_tag="OO335_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR6003397.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14092..>14479)
                     /locus_tag="OO335_003108"
     CDS             complement(14092..>14479)
                     /locus_tag="OO335_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6003398.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3860 a         3651 c         3418 g         3550 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaacttctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt tttgtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caggatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatctggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccactgtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgcta
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgatactcat taaagtaaat attcccgcgg
    13861 atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag agcggaataa
    13921 caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct ttacctgcgg
    13981 acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca agctgagcaa
    14041 taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac atcatctctc
    14101 atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt cctttgcgtc
    14161 gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc cagtggcatt
    14221 ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt caatgtgacc
    14281 gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc ctggcttcat
    14341 tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga ctggtactag
    14401 tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc ggtttgcgac
    14461 aatgccatca atcggcgca
//
LOCUS       DAJTSF010000093        14316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000093 DAJTSF010000000
VERSION     DAJTSF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.093"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..3051
                     /locus_tag="OO335_003109"
                     /pseudo
     CDS             1..3051
                     /locus_tag="OO335_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3061..6707
                     /locus_tag="OO335_003110"
                     /pseudo
     CDS             3061..6707
                     /locus_tag="OO335_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6768..7175
                     /locus_tag="OO335_003111"
     CDS             6768..7175
                     /locus_tag="OO335_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003399.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7717..8805
                     /locus_tag="OO335_003112"
     CDS             7717..8805
                     /locus_tag="OO335_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR6003400.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8816..11095
                     /locus_tag="OO335_003113"
     CDS             8816..11095
                     /locus_tag="OO335_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003401.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11088..12224
                     /locus_tag="OO335_003114"
     CDS             11088..12224
                     /locus_tag="OO335_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6003402.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12221..14224
                     /locus_tag="OO335_003115"
     CDS             12221..14224
                     /locus_tag="OO335_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003403.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3546 a         3754 c         3655 g         3361 t
ORIGIN      
        1 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
       61 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      121 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      181 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      241 atctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      301 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      361 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      421 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
      481 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
      541 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag
      601 tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag
      661 cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat
      721 gccagcacca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccatag
      781 tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
      841 cgccattagg catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
      901 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
      961 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     1021 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     1081 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggagg
     1141 ctggagaaag cagtaaaagc atggaatgcc cttactctat ctccacatac cgataccgaa
     1201 tacgccatgt tacattttgg actcaaaggt ttacctggtt tagttaactc aattacacgc
     1261 tatccacagg aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc
     1321 gtcgcccgtg ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac ctggctgttg
     1381 aaatacccgg aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa
     1441 gcacaggata acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta
     1501 ctgcaagaaa tcgcccggcg ttataaccag tcggaagtaa cagacgcggt gaacgctctg
     1561 cttgcgctcg atcccttaga taatcacccg acaaaaatcc ccactcttcc gaccttttat
     1621 cagccatcga tctggacgcg cccggtatta aaagcaaacg cccaatcact gccagataac
     1681 gccctcctcc acctcggcga aatgctccgc ttccctcagg aagaggctct gtatccggga
     1741 ttattgcagg tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc ctgggatctg
     1801 tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta
     1861 ggcgttctcg gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct
     1921 ggtgaatccc agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt
     1981 gatatcgccc tgatgcagct taacggcatt gctcagaaac tgaaattcaa agcattacag
     2041 gagcgggcaa aagaaaagat tgccgacatt gccgagagcc gcgaactcac ggtagcggag
     2101 cttgaagatc ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat
     2161 tttggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat
     2221 gtttccggca gccgcctgaa agacctgcca aaaccaaaca aaagcgatta tgagtcacag
     2281 gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgcggcc
     2341 cagcaggtag caaggcttga atccgccatg tgcctgcgtc gccgctggtc gccggaaaac
     2401 ttccaactct tcctggttgt gcatccgctg gttcgccact taacccgccg tctgatttgg
     2461 ggcgtttata gcgccgacaa ccagctactg gcctgtttcc gggtagcgga agataacagc
     2521 tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcatt
     2581 cctcacgttc tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac
     2641 tacgaactgc taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc
     2701 gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc
     2761 atggggctgg caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatgatc
     2821 aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtata
     2881 tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
     2941 gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
     3001 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
     3061 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
     3121 ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa
     3181 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt
     3241 gtctggcgtt atgaaaattt ttcaccggag tggcagtccg ccattacgca ggccatcaac
     3301 ttgatcggcg aacacaaacc ctcagtcccg gcccgcacaa tggcggcact agcctttatc
     3361 gcgcaaaatg acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat
     3421 gcgactgagg tggtgattgc acgccagttt attgcacggt gttatgagag tgatcctctg
     3481 gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc agaaacctat
     3541 aacgaattcg atctccgact gcgtaaacac ctctctctgg cagaggaaag ctgctggcag
     3601 cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt
     3661 attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag ccttgaatgc
     3721 ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctactga ccccaaagcg
     3781 gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga agccagctac
     3841 atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga
     3901 ctggcagtcg ttccgcgcct cgcgatatat gcccataaag aagattgcgg cagtctgctg
     3961 gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa
     4021 cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac actcgccgca
     4081 ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc aatcatcgaa
     4141 gacaaaaagc tgcctgcaca gcaaaaagct cgagatgaat actggcgtac gctgttacaa
     4201 acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt aagtactcaa
     4261 gcccgggcag tgctgaatag ttatttatcg gcaccgccca aaccagttat tgatagcacc
     4321 gataacagcc aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag aaagaaaaag
     4381 acttccatac gtcacttttc gctgcctgat cttacgcttg caccgcaaac gtacaggtcg
     4441 ccgaataaac actggacgcc ggaacagcaa actgcaattg accattttag cgccatgtct
     4501 tttgacgaac gtcttgctac gagaggagcg gatatcttct tgcgggaatt aggttttgac
     4561 gttcatagcc tggaatttaa taagcgtctt cttaatggac agttggatgc ctttgatgct
     4621 cagcaactgc aagaatacac atcaatagga ctattgcgta cttatcacgc gcatcaggcc
     4681 aaatctaaac agtaccggga agacgccacc aatgcattac tggcacaaga tagcattgcg
     4741 ttgatgggag ggtggcgaac ttttcagcac ggattttact atggtaatga acagcgttgg
     4801 gggatttgga atctgtacct tatcgcgcag atgccgcgcg aaatggcggt atcgtgctgg
     4861 caacaaattg tcgaggctga ttttcattac accggggtgg aatatttgtt aagtgtgctg
     4921 ggtaccgatg ctttgcctgg attaaacacc gcctttgccc gacatccaaa agagatattt
     4981 ccgctgctga ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg cgtctggcac
     5041 ctttttgcgg gccagcgtaa tctggctcgc cagtggattt tacaatggcc ggaacatacg
     5101 gctactgcac ttatccctct tgtcttcacc aaatctagcg ataaaagcga agctgcatta
     5161 cttgccctgc gtttacttta cgaacatggg catggcgaat ggctacaaac tgtagccaat
     5221 cgttggcagc gtacagatct atggcctgcc ctggaacaat tacttaaaca gagtccaata
     5281 gagatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccgca aatgtggtcc
     5341 aggccgcgcc ttatcactaa taatcaacct gttaccgatg acgctctgga aattatcggc
     5401 gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg
     5461 ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa
     5521 gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa
     5581 agcacggcac gggatctaac gacacatatc ctcgcctggc cacaagaagg caaatctgcc
     5641 cgtgcggtca gtggcatgaa catccttacc ctgatgaata atgatatggc gctgatgcag
     5701 ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc ggcggaattt
     5761 cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc
     5821 ccaacactgg gccttaatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt
     5881 accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag
     5941 aaaagtgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc
     6001 cgactaaaag ggttaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt
     6061 gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt
     6121 aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttat ccggcaaatg
     6181 aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc
     6241 aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa
     6301 tgacagcaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc
     6361 cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca
     6421 gcctgactcg ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca
     6481 aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg
     6541 tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct
     6601 tccgttctct tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt
     6661 ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaac aaagcgggca
     6721 gtgatgcgac tgcccgttat ctacacgact taccagcggg gaaagcgatg atcgtgcaaa
     6781 aagagctggt tgctatttac gattatgagg tccctgtacc tgaagatccg ttttccttca
     6841 gacttgagat ccataaatgc tctgaattat ttacaggttc cgtctaccga ctggaacggt
     6901 tccggctacg tccaacattt catcaacgtg atcgagagga tgttgacccg ctaataaatg
     6961 atgcgttgat ttatataaga gatgagttta ttgatgagca aaaattacga ggcgaatcac
     7021 ctgaacctgt aatagtaatt tttaattgta aactacagaa tatattcaac caacaaatag
     7081 aattatatac tctagataat tcaagtcgca gcacatgcaa cttgaagtat gacgagtata
     7141 gctttattaa aggggggaac ggtattcccc cttagatgcg tttcaatttg cccgttactt
     7201 caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt
     7261 taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcacaac accagcacct
     7321 tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg ttgcactccg
     7381 gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa taggttcgat
     7441 ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg caacactgtc
     7501 cagaggtggt tataattcgt tcgttggaac acgatgttgc acaggcagtg gtgttaggcc
     7561 tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta ctgtgatctg
     7621 ctttccagat atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
     7681 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
     7741 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
     7801 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
     7861 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
     7921 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
     7981 aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc
     8041 cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt
     8101 atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
     8161 gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt
     8221 ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt
     8281 cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
     8341 gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
     8401 atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
     8461 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
     8521 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
     8581 accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat
     8641 cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac
     8701 gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa
     8761 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
     8821 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc
     8881 gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggccctgctg attttaatga
     8941 ccgggtagac gaactgtttt tagcccacca gcttccggta gctatttaca gttattgcca
     9001 gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc
     9061 ggagtggcag gcgctacaag ccgatcgtcg tattcaggca caaacttact tcatcgattt
     9121 gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca
     9181 gaccttgctg ctgcgtgcca cccgcatgga taacagcgac accctgtggg atcacttgtt
     9241 cgaagatgaa agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact
     9301 gcggggcgac tcccccggcg atgctctaaa tcgtcagcgc gaagccttta tggcccgctg
     9361 gattggatgg gcgatgcagc aaaataatgg cgacgtgtta gttgtctgcg gtggctggca
     9421 cgctccggca ctggcaaaaa tgtggcgcga atgtccgcag gtaattaaca cgccagaatt
     9481 gccgtcgctg gcagatgccg ttacaggttg ttatctcacc ccctacagtg aaaagcgcct
     9541 tgatgtgctg gcaggatacc tttcaggaat gcctgccccg gtctggcaaa actggtgctg
     9601 gcagtggggc ttacagcagg cgggagaaca actgctaaaa acggttctca cccgtttgcg
     9661 ccagcacaag ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc
     9721 actggcacag ttgcgcggtc atacactacc gttacgcact gactggctgg atgccatagc
     9781 aggatcgctg attaaagaag ccctgaatgc gccgttgccg tggagctatc gcggcgttat
     9841 tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt
     9901 cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca cttgcgaact
     9961 ggaacgtacc gcaatctctc ttccggcgga gcttacctta aatcgcttta accccaatgg
    10021 actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg
    10081 ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg
    10141 cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactcctgga
    10201 agccgcacgc cataaattag aagccgatat gctggacgcg ggtggaatcg gcagcattac
    10261 cacatgtctt agccaagcgg cgtcagcggg tctggcgtcc ttcagtcaac aattactgga
    10321 gcaactcaca ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga
    10381 agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggcg cgcagatatt
    10441 acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ccaacggcag
    10501 accggatgaa aaggagtttc acgctcactt gcatagctgg caagcgttgt gtcatatcct
    10561 gcgcgatcta catagcggtg tgcaactccc cggcgtttcg ctttctgcgg cggtagcctt
    10621 gctggagcga cacagtcagg cgattcatgt cctggcactg gatcgcggcg cgactcttgg
    10681 tgcgctgatg cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc
    10741 gcagttatcc ccggcacaat ccggcgaggc gctgcacggt ttgctggcat tagcccgtca
    10801 tcaactggcc tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaactaag
    10861 tgatgccgat tttaccaatg ccctgcccga tttacgcgcg gcaatggcct ggctaccgcc
    10921 acgagaacgc gggacgctgg cgcatcaggt gcttgagcat tatcaactgg cgcaacttcc
    10981 cgtttcggca ctgcaaatgc cgttgcattg tccaccgcaa gccattgcac atcatcaaca
    11041 actcgaacag caggcactgg catcgctgca acactgggga gttttccatg tctgaactga
    11101 acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag
    11161 aaacgacgct ttgtgggctg gatgacaacg cccggcagat agatcacgcg ctggagtggc
    11221 tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctctagtgga ttaggtggct
    11281 caaatctcac cactcctgag tggatcaaca gtattcacac gctgtttccg caacaggtga
    11341 ttgagcggct ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacaaatc
    11401 tcgacgtgct ggaacgtatg cagccttccg aaagcctgct acgcgccgtt ttgcacacca
    11461 aacatctgat gaaccccgaa gtactggctg ccgcccgcag gattgtgcgc caggttgtgg
    11521 aagaaattat ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc
    11581 gtcgtcgccg ctcatttatt ccactggcgc gaaactttga tttcaaaagt actctgcgcg
    11641 ccaacctgca acactggcac ccgcaacacg gcaagttgta tatcgaattc ccccgcttta
    11701 acagccgcat taaacgccaa agcgagcaat ggcaactggt cttactggtt gatcaaagcg
    11761 gatcgatggt cgattcggtg atccactctg cggtgatggc ggcctgttta tggcagttac
    11821 ccggcattcg tacccatctg gtggcgtttg acacaagcgt cgttgatctc acggcagacg
    11881 ttgccgatcc ggtagagtta ttaatgaaag tgcaattagg cggcggaacc aatatcgcca
    11941 gtgccatgga gtatggtcgg caacttattg aacaacctgc aaaaagcgtc attatcctcg
    12001 tgagcgattt ttatgaaggg ggttcatcat cattgctgac gcaccaagtg aaaaagtgtg
    12061 tccagagcgg catcaaagtg ctgggactgg cagcgctcga tagcacagca acaccttgct
    12121 atgaccgcga tacggcccag gcgctggtta atgtcggcgc acaaatagcc gccatgacac
    12181 cgggcgagct ggcatcatgg cttgcggaga atcttcagtc atgaattcac tacgtccgga
    12241 attattagaa ctgacaccgc aggcactgac ggcgttaagc aatgccggtt ttgttaagcg
    12301 cagccttaag gaactggaaa atggcaacgt cccggaaatc agccatgaga acagcgcttt
    12361 aatcgccacc ttcagtgacg gtgtccgtac ccagctggcg aacggccagg cactgaaaga
    12421 ggctcagtgc acctatgggg ccagcggcat gtgccgtcat cgcgtgatgc tggtgttaag
    12481 ttatcaacga ctttgtgcta ccgcccagcc tacgggaaaa gaagaggaat gggatccggc
    12541 aatctggctg gaagaactgg ctacccttcc cgatgccacc cgcaaacgcg cacaggcgct
    12601 ggtcgctaaa agcatcacca ttgagttgtt ctgtgcgccg ggtgaaattc cctctgcccg
    12661 cttaccgatg agcgatgtgc gtttttattc ccgcagcagt attcgtttcg cccgttgtga
    12721 ttgtattgaa ggcacacttt gcgaacatgt cgtactggca gtacaggcct ttgtccaggc
    12781 aaaagcgcag caagcggaat tgactcattt aatctggcag atgcgcagcg aacacgtcac
    12841 atcatctgac gatccgtttg ccagcgaaga aggcaaaacg tgtcgtcaat atgttcaaca
    12901 actaagccag gcattatggc tgagcggcat cagccagccg cttattcact acgaggccgc
    12961 tttcagccgc gcgcagcaag cagcggaacg ctgcagctgg cgatgggtga gtgaatcgct
    13021 acggcaactg cgcgccagcg ttgatgcctt ccacgcccgc gccagccact atcatgccgg
    13081 agaatgctta cgtcagcttg cggcattaaa cagtcgatta aattgcgcac aagagatggc
    13141 ccggagcgac agtgttggtg aagttcctcc tgtgccgtgg cgcacggtcg ttggctctgg
    13201 cattgccgga gaagcaaagc ttgatcatct gcggctggtg tctttaggta tgcgttgctg
    13261 gcaggatatt gagcattatg gtttacgcat ctggtttacc gatcccgaca ccggcagtat
    13321 tttgcacctt tcgcgcagtt ggccgcgaag tgaacaggaa gactcaccgg cagctacgcg
    13381 tcggctgttt agttttcagg ctggcgcact ggcgggtggg caaattgttt cacaagcagc
    13441 aaaacgcagt gccgatggcg agctgctgtt agctacccgc aaccgcttaa gcagcgttgt
    13501 accgctgtcg cctgatgcct ggcaaatgtt gagcgcgccg ttacgccagc cgggcattgt
    13561 ggctttgcgg gaatatttac gccagcgtcc ccccgcctgc atacggcctc ttaatcaggt
    13621 cgataactta tttattctgc cggtcgctga gtgtatttcg ctcggttggg atagcagccg
    13681 ccagacgctg gatgcgcagg taatcagcgg cgaaggtgaa gataatctgc tgacgttatc
    13741 actgccagcc tcagccagcg cgccttatgc cattgaacgc atggcggcgc ttttgcaaca
    13801 aacagacgac cccgtgtgtc tggtttctgg ctttgtcagt tttgttgatg ggcaattgac
    13861 actggaacca caggtaatga tgacaaagac tcgtgcctgg gcgctggacg cagaaactgc
    13921 gcctgtggtg gtatcactac cttctgccag cgttctgcct gtgccgtcta ctgctcatca
    13981 gttgctgatg cgctgccagg cgttacttat tcaactgctc cataacggct ggcgctatca
    14041 ggaacagagc gctattaatc aggcagagtt gctggcgaat gacctcaccg cggtgggttt
    14101 ttatcggctg gcacatgtgt tggcacaatt tcgtaataca gaaagcgagg cacgggtaga
    14161 agcaatgaat aacggtgttt tgctttgcga acaattattc cccatgcttc agcaacaagg
    14221 atgaaatagt gctttttact aagagttcta ctccagttcc ggactgctca cgccacggta
    14281 ttaggcatat cctatatagc ccctggtgag agtcac
//
LOCUS       DAJTSF010000094        14102 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000094 DAJTSF010000000
VERSION     DAJTSF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.094"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            6..407
                     /locus_tag="OO335_003116"
                     /pseudo
     CDS             6..407
                     /locus_tag="OO335_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..4690
                     /gene="pic"
                     /locus_tag="OO335_003117"
     CDS             572..4690
                     /gene="pic"
                     /locus_tag="OO335_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCR6003404.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4841..5083)
                     /locus_tag="OO335_003118"
                     /pseudo
     CDS             complement(4841..5083)
                     /locus_tag="OO335_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5122..6072)
                     /locus_tag="OO335_003119"
                     /pseudo
     CDS             complement(5122..6072)
                     /locus_tag="OO335_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6123..6698)
                     /locus_tag="OO335_003120"
                     /pseudo
     CDS             complement(6123..6698)
                     /locus_tag="OO335_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6702..7102
                     /locus_tag="OO335_003121"
                     /pseudo
     CDS             6702..7102
                     /locus_tag="OO335_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7505..8086)
                     /locus_tag="OO335_003122"
     CDS             complement(7505..8086)
                     /locus_tag="OO335_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003405.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(8133..11990)
                     /gene="sigA"
                     /locus_tag="OO335_003123"
     CDS             complement(8133..11990)
                     /gene="sigA"
                     /locus_tag="OO335_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR6003406.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(12351..13355)
                     /locus_tag="OO335_003124"
                     /pseudo
     CDS             complement(12351..13355)
                     /locus_tag="OO335_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            13340..13717
                     /locus_tag="OO335_003125"
                     /pseudo
     CDS             13340..13717
                     /locus_tag="OO335_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13756..>14102)
                     /locus_tag="OO335_003126"
     CDS             complement(13756..>14102)
                     /locus_tag="OO335_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6003407.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         3540 a         3315 c         3457 g         3790 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgtccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgggta gtggtacgca gtacactaag gacaaggacg gaaatttagt
     1201 taaggttgcc ggtggatatg cttttaaaac aggaggaacc acaggagttc ctctgatatc
     1261 tgatgcaaca atagtctcta atcccgggca aacttataat cctgtaaacg gccctttacc
     1321 tgactatgga gcccctgggg atagtggttc tcctttgttt gcttatgata aacaacaaaa
     1381 aaaatgggtt attgttgctg tattaagagc atatgcaggt attaatggtg ctacgaactg
     1441 gtggaatgtc ataccaacag attatctgaa ccaggttatg caggacgatt tcgatgcccc
     1501 cgtagacttt gtttccggac tgggccccct gaactggaca tacgacaaaa catcaggcac
     1561 aggtaccctg agccagggca gtaaaaactg gaccatgcac gggcagaaag acaatgacct
     1621 caatgccggt aaaaatctgg tattcagcgg gcagaatggt gcaattatcc tgaaagacag
     1681 tgtgactcag ggtgccggtt atctcgaatt taaagacagt tacaccgtat ctgctgaatc
     1741 cggaaaaaca tggacgggtg ccggcattat tactgacaag gggacgaatg taacctggaa
     1801 ggtcaacggc gttgccggtg acaacttgca taagctgggg gaaggaaccc tgaccataaa
     1861 cggaacaggt gtaaacccgg gaggactgaa aacgggagac ggtatcgttg tacttaacca
     1921 gcaggcagac actgcaggta atatccaggc cttcagttca gtgaacctcg ccagcggacg
     1981 tccgaccgtg gtgctcgggg atgcccgtca ggtcaatccg gataacattt catggggata
     2041 ccggggaggt aagcttgacc ttaatggtaa tgccgttacc ttcacccgac tgcaggctgc
     2101 tgattacggg gcggtgatta caaataatgc acagcaaaaa tcccagcttt tactggatct
     2161 taaggctcag gatacaaatg tcagtgaacc gacgattgga aatatatccc cctttggtgg
     2221 taccggcact ccaggaaacc tgtacagcat gatactcaac agccagaccc gcttctatat
     2281 tctgaaatct gccagctatg gtaacactct gtgggggaac agcctgaatg atccggctca
     2341 gtgggagttt gttggcatga acaaaaacaa agcagttcag acagtaaaag ataggatcct
     2401 ggccgggcgg gcaaaacaac ccgttatctt tcatggtcag ctgaccggga atatggatgt
     2461 cgccattcca caggtgccgg ggggaagaaa ggtaatcttt gatggtagcg tgaacctgcc
     2521 ggaaggtacc ctgagtcagg acagtggcac cctgatattc cagggacatc cggttatcca
     2581 tgcctccatc agtggcagtg caccggtcag cctgaaccag aaagactggg aaaaccgtca
     2641 gtttacaatg aaaacactgt cgctgaaaga cgctgacttc catctttcac gtaacgcctc
     2701 gctgaacagt gacattaagt cggataacag ccatatcaca ctgggaagtg acagggcatt
     2761 tgtggataaa aatgacggaa caggaaatta tgtcattccg gaggaaggta cctctgtccc
     2821 ggacaccgtg aatgacagga gccagtatga agggaatatt acgctgaacc ataactcagc
     2881 cctggatatc ggcagcaggt tcaccggggg gattgacgct tatgacagtg ccgtcagcat
     2941 cacctctccg gacgtcctgt tgacagcccc gggtgctttt gccggcagtt cactgacagt
     3001 gcatgatggc ggtcatctta cagcactgaa cggtcttttc agcgacgggc atattcaggc
     3061 cggtaagaac ggcaaaatca ccctgagcgg tacaccggtt aaagatacgg ctaatcagta
     3121 tgcccctgct gtatatctga cggacggata tgacctgacc ggcgataacg cagcactgga
     3181 aattacccgt ggagcacatg cttccggtga tattcatgcc tctgcggcat caacagttac
     3241 catcgggtct gacacgccgg cagaactggc ttctgcggaa acggctgcat cggcgtttgc
     3301 cggcagtctt cttgagggct ataacgcagc attcaatggt gccataaccg gtggcagggc
     3361 tgatgtcagt atgcataatg cactgtggac tctgggtggg gactctgcca tccacagtct
     3421 taccgtcaga aacagccgta ttagttctga aggagaccgt acattccgta ccctgacggt
     3481 gaataaactg gatgcaacag gcagtgattt tgttttgcgt acggacctga aaaatgccga
     3541 taaaattaat gtgactgaaa aagccactgg ttcagataac agcctgaacg tcagctttat
     3601 gaataatcct gctcagggac aggccctgaa tattcctctg gtcacggcac cggcgggaac
     3661 ttcagcagag atgtttaagg ccggcacccg ggtgacaggt ttcagtcggg tgaccccaac
     3721 cctgcatgtt gataccagtg gtggcaatac gaagtggata ctggatggtt ttaaagcgga
     3781 ggctgataaa gccgctgccg cgaaggctga cagtttcatg aatgccgggt ataaaaactt
     3841 catgacggaa gttaacaatc tgaacaaacg tatgggtgac ctgcgtgaca caaacggtga
     3901 tgccggtgcc tgggcgcgca tcatgagtgg tgccggttct gcagacggtg gttacagtga
     3961 taattacacc catgttcagg tcggctttga caaaaaacat gaactggacg gtgtggacct
     4021 gtttaccggt gtcacgatga cctataccga cagcagtgca gacagccatg cattcagcgg
     4081 aaagacgaaa tcggtggggg gcggtctgta tgcttcagca ttgtttgagt ccggtgccta
     4141 tatcgatttg attggtaaat atattcacca tgacaatgat tacacaggta actttgctag
     4201 cctgggaacg aaacactaca acacccattc ctggtatgcc ggtgctgaaa cgggttaccg
     4261 ctatcacctg acagaggaca cgttcattga gccgcaggct gaactggttt acggcgccgt
     4321 gtccgggaaa acattccgct ggaaagacgg tgatatggac ctgagcatga agaacaggga
     4381 cttcagtccg ctggttggaa gaacaggggt tgaactgggc aagaccttca gtggtaagga
     4441 ctggagtgtg acggcccgtg ccggaaccag ctggcagttt gacctgctga ataatggaga
     4501 gaccgtactg cgtgatgcgt ccggggagaa acggataaaa ggagagaagg acagccggat
     4561 gctgtttaat gttggtatga atgcgcagat aaaggacaat atgcgctttg gtctggagtt
     4621 tgagaagtca gcctttggta aatataacgt ggataatgcg gtaaacgcga atttccggta
     4681 tatgttctga tgtgggggta aagggctgcc ggaaggcagc ctttctcaga gactcttaaa
     4741 atataaaact gaaggtcttc ggtaaggggg agtgtattag acgtcaacta gttttgccga
     4801 ctggcgcgag cctgtttaag attctgtgta aatgcctttc tcagaagtga ccgtccaggc
     4861 ggtcaccgaa ctcgataata aagcggctca ttgccattcg ccagtccttc aacggcatcg
     4921 tccatttctg ggacgcagac tggattgcca gccacaccac ttttttcacc gagtcgtctg
     4981 tcgggaacac tttacgcttt ttgatcgcat ggcggatcac gctgtttagc gactcgatgg
     5041 cattcgtcgt atagatcact ttgcggatgt ccgttggata agcggtaagc gtacagcgag
     5101 ggccgtattg acggggatgt gttattcagc tggcagtgct atgcgccacg gaagcagttc
     5161 gctgacccgg ttgaccggcc agtctgctat gacgccaagc acatggcgaa ggtagctttc
     5221 tggatccacg tcattcagtt tgcacgtccc gatcaggctg tacagtagcg cgccccgctc
     5281 accaccatgg tcagagccga agaacaggaa gtttttacga cccagactga ccgcccgcag
     5341 ggcattttca gcgatgttgt tgtcgatttc cacccagcca ttactgcagt acacgttcag
     5401 ggccttccac tgtttcagta ggtatgcgaa cgcttttgcc gtatccgagt gacgcgacag
     5461 tgttttcatc tgttgctgta tccggtcata cagtgactgc atcagtggcg cggctcttgc
     5521 ttttcttgcc gccagacgct gttctgctga acatccccgg acttctgcct cgatggtata
     5581 cagttcaccg atacgctgca gggcttccgt ggtgatgtcg gttggcactc ttgcatgcac
     5641 atcgtggatt tttctccggg catgggccat acacgcagct tccgttattc tgccggattc
     5701 gtataacgcc cggtaaccac cgtaagcatc tgcctgaagc acaccgctgt aaccggctag
     5761 gtgattctgt ggatggatac ctttccggtc cgggctgtac gcgaaccaga ccgccggggg
     5821 catctgtgaa ccggcgttac ggtcatcacg gacatagacc cacagccggg cagttcttgt
     5881 tttaccactg cccggctcct ggaccggaac ggggatatca tccgcatgaa ctttaccggg
     5941 catcagtaca tactggcgca ggacgtcata cagcggctcc agcagttcag caacggcacc
     6001 ggtccagcga cccagtgtgg cacggctcag ctccacgccc tgacgacggt atatttctga
     6061 ctggcggtat aactgtcaac gacggatgaa aagtggctct tccgcaacct gggtggagat
     6121 tttcatgcca gtggactcat taccctctgc ctgagtagtt caaatcctgc tcgaccatac
     6181 atctggcgtt tcagcatctt cttcaggcga tttacatgac cttcgacaac accattactc
     6241 cagcgactac tgattgcctc acatatagct gcagcatcag cttccatccc cgccgccacg
     6301 cgccgaaatt ctgctgaacc gctttcgtgg actcgagtga accaactgct tagctgtgat
     6361 ttattttggg ttttcagaat acggtagaac tcgagtgcca gttgctgcgc tattttcagc
     6421 tcaggttctt tttcacacat cagactaata aatcgggaag cataattttc ttcatccctg
     6481 gttattctcc agggcatcaa ccagcggctt acccgggaga ctgaaggaag tctgacggca
     6541 gtagtaaccg gtgggttcca gcctttacgc catttggcaa ccgcatccct gacgatggtt
     6601 tcactgcctg taaatccccg ggccaccatt tcccgccata tccggctggc gttataatta
     6661 ccactttctc gttgctcttt taaccactcc ctccatggtg tcagcgaaaa acgaaaccgc
     6721 tgttgaaatc cctggaaagc tggttgcgga aaagatgaag acccagtcgc gacactcaga
     6781 gttggcgaag gcgttcgcgt acgcacttaa ccagtggccg gcactgacgt actatgcgaa
     6841 cgatggctgg gtggaaatcg acaacaacat cgctgaaaat gccttgcggg cggtcagtct
     6901 gggtcgtaaa aacttcctgt tcttcggctc tgaccatggt ggtgagcggg gagcgctact
     6961 gtacagcctg atcgggacgt gcaaactgaa tgacgtggat ccagaaagct accttcgcca
     7021 tgtgcttggc gtcatagcag actggccggt caaccgggtc agcgaactgc ttccgtggcg
     7081 catagcactg ccagctgaat aacacatccc cgtcaatacg gccctcgctg tacgcttact
     7141 catcacctgc tattaatccc cgagtcagta cttcgcggcc tgacgcgatc accctactca
     7201 gtacttcaga gcccgcctcc ggtacctgcc tggcgatcgc ccctgtgcaa caaaataaat
     7261 caaccgttat caaaccacga tcgttcgtat gcgccgccga tgacgcgcgc ctgaaaaacg
     7321 cgtcaatatg agttccccgg aagcttacga taatacgctt atgaacatta caacacgctc
     7381 atgcggaacc tggatggggg aaggaacggt gaagctgtta gcgaatgacc ggcgtttata
     7441 caaacgccgg tcatgatgaa tgatatttca gaatatacct cgggctaaaa taccgggaca
     7501 gagatcaggc gtgattaatc tgcagcattt ttaataacag aagtactgag cttactttac
     7561 ctttgatgat gtaatagtta tttccgtcgg tactcatttt tccctgaaat aatgtcacgg
     7621 atgtttcttc ctgctgatcc tgcaggcgca gtcgtaaggt catattttta atgagctgag
     7681 atccgggtgg caaggggtat ttgctgtcag atactgggcg actgagtctg atgtggcgcg
     7741 tatacagccc gttttcgtac agggtggcct ctgttatggt gtcagtgacc agcatgtggg
     7801 agacaatgct gtattctttt ttaaagcacc caccagaaac ggtgattgta atgataatgg
     7861 tcttctcgat cgagatgtaa atacccgttt cgttatactg tccgtatcgg aaccatgaga
     7921 actcagttgc attccagcca tgctccagaa tatgtgcatt tttttgcctg atagtttctt
     7981 tttttattcg ctcatgttta aggcataaca ggcagataaa taatgtcaga aaaagaaagc
     8041 taataatgag agcaagagcg atatagtctg tcacagggta atacatattc tctccggtag
     8101 aagaagggcc gcaaacgcgg cccgggctgt tatcagaaag agtaacggaa gttggcgttg
     8161 atcgcgttat ccacgttgta tttaccaaat gccgatttct caaactcaag accgaagcga
     8221 agattatcgc gaatttcggc gttgagacca acattcatga gaatacgacc gtctttttca
     8281 cctttgatac gtttctcacc ggacgcatca cgcagtacgg tttcaccgtt ggcaaacagg
     8341 tcaaactggt agccaaggcc ggcgcgggct gtgactttcc agtccttacc ggagaaggat
     8401 ttacccacat caacaccggt acgcccaatc agcggattaa agtccttatc cttcatggtc
     8461 acgctcattc cgcgatcctg ccagtcgaac cgtttacctg atacggcccc ataaaccagt
     8521 tctgcctgcg gctcaatcca ggagtcttct gttacatgat agcggtagcc tgcttcagca
     8581 cccacataca aggaatgaga actgtagtct tttgttccga gtccagcaaa ggtcgccgag
     8641 tactcattat catggtgaac atacttacta atcaggtcga tataggcacc agagtcaaat
     8701 atagcggaag catacagacc tgccccgacg gacttcgtct tgccactgaa tgcattactg
     8761 ctggcatgac tgtcggtata cgtcatagtc agaccagtga aaaggtcaag tccatccagc
     8821 tcatgttttt tatccacacc aatctgcaca tgggtgtagt tgtcactgta tccaccagaa
     8881 gctgaacctg ctccgctcat gatgcgggcc catgcaccgg cctcgccatt ggtgtcacgc
     8941 agatccccca ttcgtttatt caggttgttg acttctgtca ggaaggcttt gtaattaccg
     9001 gacatatacg attttgctgc accggaagat gctgtatttt cagccacctt atatccggtc
     9061 agggtccacg tggtatgttc ctgatcctct ttcgtgctga tcacaggaga tatgttgctg
     9121 aaacctatgg cctgagtact ggctttaaat atctctttat tgcttccttt tggggctgtt
     9181 atcaggtcaa cgtttaatcc tgagttatta tttcccactt tctgtatatc gaccgacaga
     9241 ctgttattgc ttccggttaa ctgattttta acaatgagtt tatccgattg atttacagac
     9301 tggctcagat cagcccgcat aatcacagca gtattagtgg catccagtgt gtttactgtc
     9361 acagtatgga aacccgaatt ctctatgtta ctaaacaaca aaagactgtc gcgggttttt
     9421 aatgattgca gggctgactg tccgtttact ctccataagg tgttatacat gctggctgag
     9481 gctgcaggag catcaatacc tccggtataa gaagtatcaa agccacccag caaacctgta
     9541 gccagcggtg tgtgtgtggt tttgtccagg ttatcttctt tatactggtc aacaccaaac
     9601 agaatagatg cttttttgtc tgattttata ttaccattga cccaggcata attttgagcc
     9661 tttaacgtgg caccatcttc tgtcatatta tatccatctg acatatagat tgccgggctg
     9721 tatttattat tttctgtagg ggtgcctgtt aatgtcaggg ttccggccag ttcaatagct
     9781 ttatctacat acatttttcc ctgggcggta acagtagctc ctttgttgat aatcaggtct
     9841 gtattaatta aggataatgc accagtcata gtaacagttg atttatcacc aacagttatt
     9901 ttgctgtttt gagcactcag actgccgttg aaactacttt cgcctgctga atccccggag
     9961 cgaagctgtt gtctgaatcc aaaaccttcg ccagtaatgt ttcttcctga gaacatgtca
    10021 atgtatgcag ttttatttcc cagaacaatt tgagagtttt tagcctctat gtgtcctttt
    10081 acattagcat cccggcctat actcagggtt gcgttttcta aatttaattt ccggaaatta
    10141 aatttacgac tttcccagtc gggctgggaa aaagaggcta tctgattgtt ggacttatat
    10201 tccgtattgt aacgtttatt tacagtgctt tcctggtcct ttatggcagc agaatagtct
    10261 ttttgacata aaaaaggaat tggacagttg ttattgcctt ctttaaatat ggcatgttct
    10321 gtcgcatgtc cttgtaatgt taactgtgca ttccggacag agatatcgtt tttgatatcg
    10381 actgagccat caaagatcag attggcatta ttgttatgtt gctgggtagt attgataata
    10441 tgatttatgc ttatattacc tgatacattc ccatggtaca tatatgcatc agtattagta
    10501 agtgataatg ttgattgctt cacgtttgag ttcgttacgg ttgttcccgc atctgttgct
    10561 gcgattttct gaaatgactg gtcatagccg tttagatcca gtgttccgcc atttctggta
    10621 aaatagattt cgccattacc gctttcgctt aaagcgtctt tggcatttat ttttaccgta
    10681 cctttacctc cggccatgta aattttattg aaggcttttt cagcactaag gatgacagtc
    10741 ccattaccta ttttaaggtt atttccctgt gctattttta catccagagt accactacct
    10801 attttatgca gattatctcc ggcaacgccc ttaacattcc attcaacggt ggtattatta
    10861 tcaatatcta tcccggcacc tttataagac ttatcttttc cgtacacttt atatgtctgg
    10921 ttcttatcga atataagccc gccaatgcca agatcggtat cctgcttgag cgttaatacc
    10981 cctcctcctg agacagacag gtccttatct tttataatcg atgataactc ttgtttttta
    11041 ccatccagct gcatagtgtt tgtgaaattc ttttttaaat tgtttaccag aatattatta
    11101 aataacgagt agtttgtcca ggctatattg gtactactga taaaaccaac tgtactggta
    11161 acaccatgga aaacccattt ttgttgttct ttatcatata gataagaacc agaaccacta
    11221 tcgccaaaaa ttggtgagtt tttaaaaggg gttctgttgg tataaatgta actcttgttt
    11281 ttccagtcca gattaaaaag acttgcgctt aacaagtcgg gattatagtt ttgtccaaac
    11341 agatattgtt tgccgtttaa tatcgttgag gttgttccgg cacctgctct gaagccaatt
    11401 atttgttcct taccgttgta gtttacgcca tatcttttca tcgcatcttc gggagaaagt
    11461 gagaagtcgg cactctccgt aacccctgtt gtttccacga cgaatttatt cagacgatga
    11521 accgaaaagt cgccagatga tactctgtta cttgctttat atgctgtctg atcccatgac
    11581 tgtgttgtta ttgcatgatg ctgtgtaccg ttatgagtcg cagtaacact gtacgcacct
    11641 ccaattgatg ttgttgcacc tttgttggaa acagcagaaa aatcaggtac aggtagttct
    11701 ggaaaatgaa atatctttcc atctttcatc attaattgaa catttgttgc acccggtctg
    11761 aactctcctc tgttttgagc aaggtcaaga taatcgcggg tccatatatt atttatatcc
    11821 agcattgctg caaatgctgt ttcgccataa cttccatata ttgaggataa aataataccg
    11881 aggataactt tctttcttga tgtaccgaca ctaactttcc gggtcagttc agaaacagca
    11941 actaatccac ctgtaatatg actatatttc agtgaataaa ttttattcat acgtgaactc
    12001 cgatatttct gagttgtggt tatgggatag catttaatta caacggggaa ctatcagtca
    12061 tggtacgtaa ctgataaggg tataatgata atcactgtca gttatatttg tatgacagat
    12121 gtaacaaaat gaaaaaaaag ataagcacaa gtgattgatt ggttagatat tgatcacttg
    12181 gtgtttgtat tttgaatttt gcgatcaaaa tttgatcttt gttgtttccg gtcaatcctt
    12241 tacaggtttt ctgtattatc cgggggagtt aagtctgtcg atgtccgaca gacttttgat
    12301 gatggaaaca acatgcagcg ataatgtgca tgctgttttg caggtggaac tcagaaggtg
    12361 aactggaact gtgcgaccgc accgtaagtg ttgtccgtgc cgatcaggcc tgcaacatca
    12421 aggtgaacca catcaaacgg tgagatcccc accccggccg tgaatgcact gccgtcgtta
    12481 ccggccagat tctgacggta accggcgcgc agctgtgccc atttccaggc attaaattct
    12541 gtgccaatgg cggcaaactg acggttgctg tcggaggtga aaccgctggc cggggtcaga
    12601 tccacatcaa atgcagtggt gaacatcgcg ttgtgccagg agacaccggc cgtcacctgc
    12661 gggcggatcc tgaaggtttc cgtgataccg ttcacttctt ttgtctcaat actacgggag
    12721 atcaggttct gtgcgaccag tcccagcgtc cagttgtcat ccaggtccgt actggcaccg
    12781 atgtcggcgt tgatgccatt tttcgtgttg tggtagcggt cgcctttgaa ggcgctgctg
    12841 tcataatttc tgaccagcac gttgtagttg aacaggtcga cgcgctggaa tttggggggg
    12901 aagcccagag accatttctg cccggccgtt tccagttccg tagccagagc aatacccaca
    12961 tcagtgatca gtgccgtgcg ggcacaggcc tgtgatgtca ggcggctttt gtccacctcc
    13021 tgcccggtgc tgtttgccac ctcttccagg taagtgaggt cagcatcaga gactttaccg
    13081 tcgacactga cggtgccgtg tgatttaacc atcagtgcgg cagagaggcg atcaccggga
    13141 agggccgcca cagcagaaac gcccagctgt gcggcggcat gtgtgttacg gaagtcccgg
    13201 aggtgttctt tcaggcgtgc ggcagcctca ggaacaccgt ggttactttc gatggcgcgg
    13261 tcgaaggcct tccactcatc actgatccgg tcggcgttgt cggtgatatt atccggattg
    13321 gagagctgcg cgccgacgga cggaagcagg atgctgggct catgcgcgac gcaaaatcca
    13381 cgatgtatat atcagtacta aaagcgcgac ggcggaagaa gctctgaaac taatcggtga
    13441 actgtacgcc atagagcacg atatacgcgg gttaccggtg tctgaacgcc tggcggtcag
    13501 gcaaatgcag agtagaccgc tcctgacttc cctgtataag ctgatgcagg agaaagaaca
    13561 cacgttatcg aagaaatgtc gtctgggaga tgcgttccgg tatatcagga agcactgggc
    13621 tgcgttgtgc aacttctgtg atgatggcct ggctgaggta gataataatg cagcagaaag
    13681 agcgcttcgt gcagtctgtc tcgggaagaa aactttttcg aggatcactc attccgtgtc
    13741 tgactgcttc agggtttaat tttgcttctg ctgactgaat gatgattggg cagcaagtgc
    13801 aatatcccac ataaatccag tcaactcacg agccaccgcc gtaatggcta cttgtgattt
    13861 tttccctttt gctgtcagag ttcggtaacg agaacacaat cgttgttgag ctttccatgc
    13921 aatatctttg gaatattgtg ttacagagtc cggtcggtaa tatataagcc atgctccaac
    13981 ttttgcaggt gtacgataag accaggcggc ttcgtaaagc agacgtctca gttcgctgtt
    14041 tccaactttt gttattcctc tgggacgaat actgtttcca ctggaatatt cacctggcac
    14101 ca
//
LOCUS       DAJTSF010000095        14020 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000095 DAJTSF010000000
VERSION     DAJTSF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.095"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            556..1347
                     /locus_tag="OO335_003127"
     CDS             556..1347
                     /locus_tag="OO335_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003408.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1733..1939
                     /locus_tag="OO335_003128"
     CDS             1733..1939
                     /locus_tag="OO335_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6003409.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            2025..2597
                     /locus_tag="OO335_003129"
     CDS             2025..2597
                     /locus_tag="OO335_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003410.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2599..2883)
                     /locus_tag="OO335_003130"
     CDS             complement(2599..2883)
                     /locus_tag="OO335_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003411.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            2854..2997
                     /locus_tag="OO335_003131"
                     /pseudo
     CDS             2854..2997
                     /locus_tag="OO335_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            4166..5185
                     /locus_tag="OO335_003132"
     CDS             4166..5185
                     /locus_tag="OO335_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6003412.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
     gene            5295..6167
                     /locus_tag="OO335_003133"
     CDS             5295..6167
                     /locus_tag="OO335_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR6003413.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            6501..9623
                     /gene="ag43"
                     /locus_tag="OO335_003134"
     CDS             6501..9623
                     /gene="ag43"
                     /locus_tag="OO335_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR6003414.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHG
                     SAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            9744..12260
                     /locus_tag="OO335_003135"
     CDS             9744..12260
                     /locus_tag="OO335_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003415.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            12336..12791
                     /locus_tag="OO335_003136"
     CDS             12336..12791
                     /locus_tag="OO335_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003416.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(12910..13121)
                     /locus_tag="OO335_003137"
                     /pseudo
     CDS             complement(12910..13121)
                     /locus_tag="OO335_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13202..14020
                     /locus_tag="OO335_003138"
     CDS             13202..14020
                     /locus_tag="OO335_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR6003417.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
BASE COUNT         3769 a         3469 c         3463 g         3319 t
ORIGIN      
        1 taacagacat tacttaacaa agaacattac agtcagtgtg tacattacag atttatattc
       61 aggtgttgcg ggactacatt acagcagaga ggggtaagga cagagttaca agtactacta
      121 gagagaatat cactgcacag atacttctat gagtatatct ttatgagaga tccgtctaca
      181 ggattgggtt ataaggtgta tttacaaggt ataatcacag gatcatttac atgaccatac
      241 ttctgtaaat aatactaatt aaccttaatt atttttttat ggtacatgaa ctacctgtag
      301 ctgtaacgat aaacaaatca cccaaaatga aaaatactgg ttatcacagt tcagagaact
      361 cgtatgagtg tccccctgag caatgcttca ttttctgtga ttctgtgtga tagagttata
      421 ctgagtacag tggcagtaac actgctttcc atcacttgag gataaaattc attaaaaaat
      481 taattgcatg aattaacagg tttttttatg aaatgttatg catctcaacc aatatgatta
      541 acagaaaact tcatcatgac aacacgtata acgttcagta tgaccagtga tgagacgctg
      601 cgaattgtgg atgagtactg tcatacccac aaactttcgc gctctaaggt aatcaacgca
      661 ttactcagcg ccacagcacc tgtccttaat gatattaact gttattatca acttgcaggc
      721 gaactgcagt cacgcttatt aaatggagtg tatcaacggg atcttcctcg caaacgcaat
      781 gtcgtcagtg cagagaagta ttgtctggaa atctgggaaa acaaactttt caccagaaga
      841 atcctggagt ttgacagttc aaatggtgtt ctgtacgcgc tgaaacataa aagacactat
      901 cgccgggata agatgattgg aagggttgaa agtcggtgta tcaaagacat ttgcgaatac
      961 cagatgcagc tatccgggga gaagacgaaa tatgcctgtt ttatttatat cgaaagaaca
     1021 atatacaatc acgataatcc atctgataaa atacctgtaa aagcggctgt tggtaatgca
     1081 gtcatcctgc tggcaaagga tgtgatatat aacgagtact tttttgattt gcggcagtca
     1141 ttttttgtga gcgttacaga tctgatggtc tctggcgcaa aaggtattcc ggagacacag
     1201 acatatccgg acgtttattg ctggatccct cttttttcca taaattccgg cgtgctcatt
     1261 acccctgtat ataaaattga ccctctgaaa cccgtaacgg taaaaaaacc ggataagatc
     1321 actgttgttt gtaattacag agaataattt cagccttcta ccttcaccgc gacagtgttt
     1381 acctctgctt tatcttccgt tcagtaaata caaaccaccg ctctggcagg tacagggcgt
     1441 ggtttatctc cgatactccc ctgtgcagta ataatcatca ctgaaaatac tgatcccgtc
     1501 acggcaaaac gccatcccat attccggcaa cacaaaatca tcttccgttt ataccggatt
     1561 cactgacaaa acagacaacc tgtattgcct gtacacaaca gtttctctcc tggcaggaat
     1621 attcccctac tcatttttta cgtttctccc cggttgttat gccggtgaag gtggtgcgtc
     1681 gttttcatca ccaacgccgg ttgagattaa caacatccgg aggaacattc tcatgaatac
     1741 cccagtttca ctgatggatg accagatggt cgacatgtcg tttatcactc agctgaccgg
     1801 cctgaccgat aagtggtttt ataaactcat caagcatgga gcctttccgc cccccatcaa
     1861 actgggccgt agctcccgct ggctgaaaag cgaagtggaa gcctggctgc aggcgcgtat
     1921 tgcacagtcc cgtccgtaat tcctgaccgt tatccgttcc accgcagcaa aggcatgatc
     1981 cggcccccgg aggcattttg ctgcgcgcta aaggagagaa tattatgcaa caacttgaaa
     2041 acaggtgtga cctgctgctc atccagcatc agaaatggat gacaagcgtg acgaggctta
     2101 tcgtcgctca tggaatgggg tctccgcatc ttcatggtta tcaccgtctg accctggcac
     2161 acttttttct gccggaaaaa ggctccgtca tttcggtggc cccccaggga ctctatcagg
     2221 tcgtcaaccc gggaacacca ccgttcattc ctgccattca ggaagggtta atgaccagca
     2281 ttcagaccca tgaaatcatg ctgcttacac attttaatct gggcggcgtt ttactcagtg
     2341 agctacatcg tctgggtgaa aaccggctgg caaacaggct gaacagcctg ctccggcgtt
     2401 tcgacgatcg tgatctctac cacacgctga tatggctgtg ctggtatgat ctgatgtgtg
     2461 cccactccat gcagccctgg actgaagagt taaagcataa atcacatgca gagcttgaaa
     2521 actgggctgt ggcgcgcaaa cgggaaaagc gcgagctgga actgatgatt gacgagtacc
     2581 tgttatacgc ctgctgattc ataacgttcc cggggttcct tcctcatcgt tacacacagc
     2641 cggacggcta tctatggctg tccgccaaat gaccatcgcc gcatttcgtt ccgctgaaaa
     2701 cggtacgtag tcagtgatac cggaatttcc ggagagggcc gcaggtatat tgcttctgtg
     2761 caattgatgc ttatttacat tggatgtagt tatttctgca tccaatgcta tcggaaagag
     2821 attaaccagc aaatggttcc tcaaatcgta ttgatattta cccggcgtat tgagcaccac
     2881 catctgacac tccgttcccg catcaaacga ctggaccgaa aaacgattta cttttcacgt
     2941 gctgtggaaa ttcacgaaaa aattattggg tcattcatcg aaaaacatat gttctgatga
     3001 ctggagtcat cctccagaaa tatacgtcaa ctgaacaata tcgatttttt atgggattct
     3061 atacactttt ccatatccct ctgaaaatca gtcatcagct caccaacacc tcttctgaaa
     3121 cttgtattaa acttattgct taaatagatg atataacatc tcttccattg cactgaatcg
     3181 gcgaaaactg ccgtttcaat aatctcaaca atattattta tcggctaact ttatcagtaa
     3241 acaatggaat gtcacactga tgaaatgaac attcagagta agatgataaa attcatataa
     3301 acgccattgc tgaatattat ttatttcata cagataaacg ataaaataca aaagggtata
     3361 tttctcaatg taacagtcaa ttattcatct ccactggcga gcacggtcag cacacaaaca
     3421 aagttagtcc tcctgcttaa tcattgcgta tcagaaacta aaaaagaaaa aattctgcag
     3481 acaacttaac aatcttcatc acatttcctg aagacagaca tatccacaaa cggtagtcag
     3541 cgttgaatta acaagtctta cagagctata cgtagcttat aaagccactc aatgttcatc
     3601 atcacccgac tacttatctg gaataccacc agtataaact catcaataaa aacacctgtt
     3661 gttaaaataa gaatactgtc agcagtaaat tattaaaaca acatctgtcc acactatctg
     3721 ttcttttaac gccaacggca gcgaaaatag tgcatcacag ccttcttttt tctttcgcca
     3781 ccactcagac aaacacaaca ctcacaccca caccacaaaa cagaatcaaa acataaaatt
     3841 aacaataaca gtcaaaagga aaacacagaa tattgaggtg gactgtcacc aggacagcgc
     3901 atacgacgca tgtgatcagc acatcccgtt cttcgctggc atccccaaaa tgcagaacaa
     3961 acaatcataa cattatgaat ataaaagaaa atatgatttt tcaccatgaa aaaaacatca
     4021 atgtatctct gtataaaaat aatgataaca tgacataaac atttaaaaac ataaataaac
     4081 agcaacaaaa acatttgata atcacttagt gattaaatac aattaagacg attttgttat
     4141 aagagaggca ccatggagtg agataatgaa taacgaacag atgcttatca aatgcagaga
     4201 tccccgtaaa tgtcggtttt atgccgaata tctgaatcac aaatacccta atgaattccc
     4261 tcactttgcg cctgacaacc cacaaaattg tatcaggaat gttaatttat ttttaaagga
     4321 aaagtattgt aatgtttcac ctcccccctt tggtactttt ggtactactg aagaagaagc
     4381 actcaataac gaaattaatg agcttgaaat tattgccggg caggcaatac tgcctgaaga
     4441 gagttttctc tggttgaaaa ctgacgaact tgcgactttc tttacctgga ctactattta
     4501 tctgtcgaag gaagaccata caaaattccc gaaaataaac cataattcat caggaataaa
     4561 aaacggaatt gaagtcagga aaatacgaac agttaccgat gaaacgttaa acacttacaa
     4621 aaaaagtaac cttccggata atccatcatc ccataacgaa cgaataaaag taatcatttc
     4681 atattttgac tcctgccatt ctttaattaa taaaaatgca aaaagcatat acctcgagga
     4741 actcaggcag gcgtggctta aaaagaaaag cgcttgtcaa aagtttaagt ggctccttcc
     4801 tgacgatgat gaaatgtgtc agtggttctg gacaagactg aaacaaaaac aggatgaaaa
     4861 acatccagta cataacccag tgacccggtg gttcatacca tcttcaacga aagaatatta
     4921 tcttgcctct cttgtcgcct ttgctttatg gaaagaagcg cctgatacca ttgaactgtt
     4981 cagaacacga ataaatcatg cctggcatca gaaaaaacaa aaggacaaat atcggagtga
     5041 tggtaaaaag gccattaata tacatataag aagcaatgtg aaagaaatgc ttgatgagct
     5101 cagaactgtt tacggtctga gaacaggaga gttttttgaa atgctgatta cagagaaata
     5161 tcttgaacac aagcgaagga agtaattcat tacctgttac attctaaata atctcagaca
     5221 gatattatct ccgtgaatcc cccgccacct ttccggtgcg cggggttttg tcttttttca
     5281 ccgggaatac atgtatgaat ccgtctgatg ccattgaggc aattgaaaaa ccgctctcct
     5341 ccctgcctta ctcactttcc cgtcacatcc tggaacatct gcgcaaactc accagtcacg
     5401 aacccgtgat tggcattatg ggtaaaagcg gggccggtaa atcttcactc tgtaatgcac
     5461 tgtttcaggg ggaggtcaca ccggtcagtg atgttcacgc cggcacccgg gaagtgcagc
     5521 gcttccgtct gagtggtcac ggtcacagca tggttatcac tgacctgccc ggggtgggcg
     5581 agagccggga cagggatgca gagtatgaag ccctgtaccg tgacattctg cctgaactgg
     5641 acctggtgct gtggctgatt aaagccgatg accgggccct gtctgtggat gagtatttct
     5701 ggcgacacat cctgcagtgc ggacatcagc aggtgctgtt tgtggtgacg caggctgaca
     5761 aaacggaacc ctgccatgaa tgggatatgg caggtattca gccttctccc gcacaggaac
     5821 agaacattcg tgagaaaacg gaggcggtat tccgtctgtt ccggcccgta catccggttg
     5881 ttgccgtatc ggcccgcacc ggctgggaac tggatacgct ggtcagtgcg ctcatgacag
     5941 cgcttcccga ccatgccgcc agccccctga tgacccgact gcaggacgag ctgcgcacgg
     6001 agtctgtccg ctctcaggcc cgtgaacagt ttaccggtgc ggtggaccgg atatttgaca
     6061 cagcggagag cgtctgtgtt gcctctgttg tccgtacggc cctgcgcgct gttcgtgaca
     6121 ccgtggtctc tgttgcccgc gcggtatgga actggatctt cttctgaacc tgttgtggat
     6181 gatgtcctcc ctgcctctga gtctgctcac aaaagcgctg ttttcgttac tgtctctctt
     6241 gtccgtgcaa tagactgata atagaataaa acgatcgata cctattttac cgatcgttaa
     6301 tatcgatcgt tatgctaata ataactcctg tttgcaatgt gcgcagatac acacagacat
     6361 gagattcagg gaacaacaga gccacacgtc agaaacttcc gtcagctgga cctccggcac
     6421 tgtaaccctt tacctgccgg tatccacatc tgtggatacc ggctttttta ttcaccctca
     6481 ctctgattaa ggaaatgctg atgaaacgac atctgaatac ctgctacagg ctggtatgga
     6541 atcacattac gggcgctttc gtggttgcct ccgaactggc ccgcgcacag ggtaaacgtg
     6601 gcggtgtggc ggttgcactg tctcttgccg cggtcacgtc actcccggtg ctggctgctg
     6661 acatcgttgt gcacccgggt gaaacagtga atggcggaac actggtaaac catgacaacc
     6721 agtttgtatc cggaacagct gatggcgtga ctgtcagtac cgggcttgag ctggggccgg
     6781 acagtgacga aaacaccggc gggcaatgga taaaagcggg tggcacaggc agaaacacca
     6841 ctgtcaccgc aaatggtcgt cagattgtgc aggcaggagg aactgccagt gatacggtta
     6901 ttcgtgatgg cggagggcag agccttaacg gactggcggt gaacaccacg ctggataaca
     6961 gaggtgagca gtgggtacac gggggaggga aagctgccgg tacaattatt aaccaggatg
     7021 gttaccagac cataaaacat ggcggactgg caaccggaac catcgtcaac accggtgcag
     7081 aaggtggtcc ggagtctgaa aatgtgtcca gcggtcagat ggtcggaggg acggctgaat
     7141 ccaccaccat caataaaaat ggccggcagg ttatctggtc ttcggggatg gcacgggaca
     7201 ccctcattta cgccggtggt gaccagacgg tacacggaga ggcacataac acccgactgg
     7261 aggggggtaa ccagtatgta cacaacggtg gcacggcaac agagacgctg ataaaccgtg
     7321 atggctggca ggtgattaag gaaggaggaa ctgccgcgca taccaccatc aaccagaaag
     7381 gaaagctgca ggtgaatgcc ggcggtaaag cgtctgatgt cacccagaac acgggcggtg
     7441 cactggttac cagtacagct gcaaccgtca ccggcacaaa ccgcctggga gcattctctg
     7501 ttgtggaggg taaagctgat aatgtcgtac tggaaaatgg cggacgcctg gatgtgctga
     7561 ccggacacac agccactaat acccgcgtgg atgatggcgg aacgctggat gtccgcaacg
     7621 gtggcaccgc caccaccgta tccatgggaa atggcggtgt actgctggcc gattccggtg
     7681 ccgctgtcag tggtacccgg agcgacggaa aggcattcag tatcggaggc ggtcaggcgg
     7741 atgccctgat gctggaaaaa ggcagttcat tcacgctgaa cgccggtgat acggccacgg
     7801 ataccacggt aaatggcgga ctgttcaccg ccaggggcgg cacactggcg ggcaccacca
     7861 cgctgaataa cggcgccata cttacccttt ccgggaagac ggtgaacaac gataccctga
     7921 ccatccgtga aggcgatgca ctcctgcagg gaggcgctct caccggtaac ggcagcgtgg
     7981 aaaaatcagg aagtggcaca ctcactgtca gcaacaccac actcacccag aaagccgtca
     8041 acctgaatga aggcacgctg acgctgaacg acagtaccgt caccacggat gtcattgctc
     8101 agcgcggtac agccctgaag ctgaccggca gcactgtgct gaacggtgcc attgacccca
     8161 cgaatgtcac tctcgcctct ggtgccacct ggaatattcc cgataacgcc acggtgcagt
     8221 cggtggtgga tgacctcagc catgccggac agattcattt cacctccacc cgcacaggga
     8281 agttcgtacc ggcaaccctg aaagtgaaaa acctgaacgg acagaatggc accatcagcc
     8341 tgcgtgtacg cccggatatg gcacagaaca atgctgacag actggtcatt gacggtggca
     8401 gggcaaccgg aaaaaccatc ctgaacctgg tgaacgccgg caacagtgcg tcggggctgg
     8461 cgaccagcgg taagggtatt caggtggtgg aagccattaa cggtgccacc acggaggaag
     8521 gggcctttat ccaggggaat aagctgcagg ccggtgcctt taactactcc ctcaaccggg
     8581 acagtgatga gagctggtat ctgcgcagtg aaaatgctta tcgtgcagaa gtccccctgt
     8641 atgcctccat gctgacacag gcaatggact atgaccggat tctggcaggc tcccgcagcc
     8701 atcagaccgg tgtaagcggt gaaaataaca gcgtccgtct cagcattcag ggcggtcatc
     8761 tcggtcacga taacaacggc ggtattgccc gtggagccac gccggaaagc agcggcagct
     8821 atggcttcgt ccgtctggag ggtgacctgc tcagaacaga ggttgccggt atgtctgtga
     8881 ccgcgggggt atatggtgct gctggccatt cttccgttga tgttaaggat gatgacggct
     8941 cccgtgccgg cacggtccgg gatgatgccg gcagcctggg cggatacctg aatctgatac
     9001 acaacgcctc cggcctgtgg gctgacattg tggcccaggg aacccgccac agcatgaaag
     9061 cctcatcgga caataacgac ttccgcgtcc ggggctgggg ctggctgggt tcgctggaaa
     9121 ccggtctgcc cttcagtatc actgacaatc tgatgctgga gccgcaactg cagtacacct
     9181 ggcagggact ctccctggat gacggccagg ataacgccag ttatgtgaag ttcgggcatg
     9241 gcagtgcaca acatgtgcgt gccggcttcc gtctgggcag ccaccacgat atgaactttg
     9301 gtaaaggcac ctcatcccgt gacaccctgc gcggcagtgc caaacacagt gtgcgtgaac
     9361 tgccggtgaa ctggtgggta cagccttctg ttatccgcac cttcagctcc cggggggaca
     9421 tgagcatggg tacagccgca gccggcagta acatgacgtt ctcaccgtca cagaatggta
     9481 cgtcactgga cctgcaggcc ggactggaag cccgtgtccg ggaaaatatc accctgggcg
     9541 ttcaggccag ttatgcccac agcatcaacg gcagcagcgc tgaaggttat aacagtcagg
     9601 ccacactgaa tgtaaccttc tgataattct gcattgcctc tctgtggtcc cggtcatgat
     9661 gaccgggacc cggacggggg aaaacgtttc agtgccacat tcactggcat tcacaataac
     9721 atgatattca tcacggagtg actatgttac agatagtcgg cgcgctgatt ctgctgatcg
     9781 caggatttgc cattcttcgc cttttgttca gagcattaac cagcacagcg tctgcgctgg
     9841 cagggttcat attgctgtgt ctgttcggtc cggctttact ggctggctat atcactgaac
     9901 gcataacccg gttattccat attcgctggc tggcaggcgt atttctgacg attgccggaa
     9961 tgatcatcag cttcatgtgg ggacttgatg gtaaacatat cgcactggag gctcatactt
    10021 ttgactctgt aaaatttatt ctgaccaccg ctctcgccgc tggtctgctg gctcttcccg
    10081 tgcagataag aaccattcag cagaacgggc tcacacctga agatatcagc aaggaaatta
    10141 acgggtatta ctgctgtttt tatactgctt ttttccttat ggcgtgttct gcatacgcac
    10201 cattgatcgc attgcagttc gatatttcac cctcactgat gtggtggggc gggttgttgt
    10261 actggctggc tgcattagtg acgctgctat gggcggccag ccagatccag gcgctgaaaa
    10321 gactgaccag tgccatcagc cagacactgg aagaacaacc ggtgctcaac agtaaatcgt
    10381 ggctgagcag tttgcaaaac gattacagcc ttcctgaaac gctgacggag cgcatctggc
    10441 tgacactcat ttcacaacgg atttcccggg gagaactgag ggaatttgaa ctggcagacg
    10501 gaaactggtt actgaacaat gcctggtatg aaagaaacat ggcagggttt aacgaacagt
    10561 tgaaagagaa cctgtcattc acacctgatg aactgaaaac gctcttccgg aaccgcctga
    10621 atttatcacc ggaagcgaat gacgattttc tcgatcgttg cctggacggc ggtgactggt
    10681 accccttttc agaaggccgc cgttttgtat cattccacca cgtggatgag cttcgtatct
    10741 gtgcctcctg cgggctgaca gaagtacatc atgccccgga aaatcataag ccggctccgg
    10801 aatggtactg ctcctctctt tgtcacgaaa cagaaacact gtgtcaggac atttatgaac
    10861 gttcttacac cggttttatt tccgatgcaa cggcgaatgg tctgattctc atgaaactgc
    10921 cggaaacctg gagtacaaat gagaaaatgt ttgcttccgg agggcaggga catgggtttg
    10981 ccgctgaacg gggaaaccat attgtcgaca gagtccgtct gaaaaacgca cggatcctcg
    11041 gtgataataa tgccagaaat ggagcagaca gactggtcag cggaacagaa atccagacga
    11101 aatattgttc aactgcagcc cgtagcgtcg gtgcggcatt cgacggacaa aacggacagt
    11161 atcgttacat gggaaatcat ggtcccatgc aactggaagt cccccgtgat cagtatgccg
    11221 gcgctgtgga aaccatgaag aataagatcc gcgaaggtaa agtacccggt gtaaccgatc
    11281 ccgcagaagc gtcccggctg attcgtcggg gacatctgac ttatacccag gcccgtaata
    11341 tcacccggtt cgggaccatc gaatcggtca cttatgatat tgccgagggg tcggttgtca
    11401 gtctggcggc cggagggatc agttttgccc tgacggcatc ggtcttctgg ctcagcaccg
    11461 gcgatcgcga tgctgccctg cagacagctg ctgtccaggc aggaaaaacc ttcacccgca
    11521 cactggctgt ctacgtcaca acccagcaac ttcaccggct cactgttgtt cagggtatgc
    11581 tgaagcatat tgatttttcg acggccagcc cgactgtccg gcaggcgctt cagaagggga
    11641 ccggtgcagg aaatatcagt gccctgaaca aagtgatgaa gggtacgctg gtgacatctc
    11701 tggcactggt agctgtcaca accagccctg acatgatcaa aatgttgcgg ggacggatct
    11761 ccggtgcgca gttcatcagg aatcttgccg tggcatcttc cggtgtggca ggtggtgctg
    11821 tcgggtcagt ggcgggcggg atattgttca gtccactggg accatttggt gcactgacag
    11881 ggcgtgtggt tggcggtgtt ctggggggaa tgattgcctc cgctgtatca ggaaaaattg
    11941 ccggagcgct ggttgaagaa gatcgcgtca aaattctggc aatgattcag gagcaggtga
    12001 catggcttgc cggcagtttc ctgctgaccg gacatgagat tgaaaatctg aacgcgaatc
    12061 tggcccgtgt tatcgatcag aatgctctgg agatcatttt cgccgccggt atacaacaac
    12121 gggccgcgac caatatgtta atcaaaccac tggtggtcag tatcatcagg caacgccccg
    12181 tcatggaata tgatgcatcc catctcggca agatggttaa ccggctggaa gaagcattcc
    12241 ccccggagct cccggcataa cgtgtgttct gctcctttta ggttttatca cctgatttgc
    12301 cagtgtcatc gtcatgactc agagaggtaa ataccatgat tcacctgttc aaaacctgca
    12361 tgattaccgc cttcattctg gggttaacgt ggtctgcccc actccgggca caggatcaac
    12421 gttacatcag tatacgcaat acagatacga tatggctccc gggaaatatt tgtgcttacc
    12481 agttccggct ggataatggc ggaaacggtg aaggatttgg ccccctcacc atcactctgc
    12541 aactcaaaga caaatatggt cagacgctgg tgaccagaaa aatggaaacg gaagcttttg
    12601 gtgacagtaa tgccacgcga accacagacg catttctgga aacggagtgc gtggaaaatg
    12661 tcgccacaac cgaaatcatt aaagcaactg aagaaagtaa cggccatcgt gtcagtctgc
    12721 cgttatcggt tttcgatccc caggactacc atccactgct gattaccgtt tccggaaaaa
    12781 atgttaactg atccctgaag tcagtttcat gcttcagcct ctgccacaac gtatggcaga
    12841 ggctatttat caggagaaac cagatatgcc gggttacaca cagagtatgt cctggctgaa
    12901 ggatctttct catacgggca ggcggttgct gtcataaccg cttaccgcaa tgtgtttatc
    12961 caggatgacc cgggaatgca tttccgtcgg gttatccgca acgcggaagg tcagcgacgc
    13021 tgacgcagcc ggaactctga agctgatgcc ggaaaacagc ttaatgcgtg gctcgccagt
    13081 ggagggcttc tcagacaata aacttcattg atttcatcca tcaggccgcg tcttctccgg
    13141 gagacgcggc ttttttcatt tgttgcacca gtaaatctta accaccgata aggagcaaag
    13201 tatgcaatta gtcagtcgtt ttggttatgc gaaccagata cgccgtgacc gtccgctgac
    13261 acatgaagag ctgatgcacc atgtgccggg gattttcggg gaagagaaac acacgtcccg
    13321 aagccagaac tatacgtaca tccccaccat caccgtactg gaaagtctgc agcgggaagg
    13381 cttccagccc tttttcgcct gccagacccg tgtgcgcgac ccgggccgcc ggggatacac
    13441 aaaacacatg ctgcgtctgc ggcgggccgg agagataaac ggagaacatg tccctgaaat
    13501 tattctgctc aactcccatg acggtacctc cagctaccag atgctgccgg gttacttcag
    13561 attcgtctgc cagaacgggt gtgtctgtgg tcagtctctg ggggaagtgc gtgttccgca
    13621 ccggggaaac gtggtggaga aagttattga aggggcttac gaagtggtgg gcgtgtttga
    13681 ccggattgag gagaaacgtg atgccatgca gtcgctggtc ctgccgccac cggcacgcca
    13741 ggcgctggca caggcggcac tgacttaccg ttatggtgac gaacatcagc ccgtcaccac
    13801 cgccgacatt ctgacgccac gacgccggga ggattacggt aaggacctgt ggagtactta
    13861 tcagaccatc caggagaata tgctgaaagg cgggatttcc ggccgcagtg caaaaggaaa
    13921 acgtatccac acccgtgcca ttcacaacat cgacaccgat attaagctca accgcgcatt
    13981 gtgggtgatg gcagaaacgc tgctggagag cctgcgctga
//
LOCUS       DAJTSF010000096        13964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000096 DAJTSF010000000
VERSION     DAJTSF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.096"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OO335_003139"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OO335_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6003418.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OO335_003140"
     CDS             2219..2767
                     /locus_tag="OO335_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6003419.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OO335_003141"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OO335_003141"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6003420.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO335_003142"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO335_003142"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6003421.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO335_003143"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO335_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6003422.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO335_003144"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO335_003144"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6003423.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OO335_003145"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OO335_003145"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6003424.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="OO335_003146"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="OO335_003146"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6003425.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO335_003147"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO335_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6003426.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="OO335_003148"
     CDS             complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="OO335_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR6003427.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3550 a         3562 c         3527 g         3325 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaattacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc ataggaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagagta cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcctggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatggt ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgct
//
LOCUS       DAJTSF010000097        13840 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000097 DAJTSF010000000
VERSION     DAJTSF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.097"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..132)
                     /locus_tag="OO335_003149"
     CDS             complement(<1..132)
                     /locus_tag="OO335_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6003428.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
     gene            315..458
                     /locus_tag="OO335_003150"
     CDS             315..458
                     /locus_tag="OO335_003150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003429.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(677..2215)
                     /gene="aldB"
                     /locus_tag="OO335_003151"
     CDS             complement(677..2215)
                     /gene="aldB"
                     /locus_tag="OO335_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR6003430.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="OO335_003152"
     CDS             complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="OO335_003152"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR6003431.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3721..5565)
                     /gene="selB"
                     /locus_tag="OO335_003153"
     CDS             complement(3721..5565)
                     /gene="selB"
                     /locus_tag="OO335_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6003432.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5562..6953)
                     /gene="selA"
                     /locus_tag="OO335_003154"
     CDS             complement(5562..6953)
                     /gene="selA"
                     /locus_tag="OO335_003154"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6003433.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(7051..7659)
                     /locus_tag="OO335_003155"
     CDS             complement(7051..7659)
                     /locus_tag="OO335_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6003434.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7730..8815)
                     /locus_tag="OO335_003156"
     CDS             complement(7730..8815)
                     /locus_tag="OO335_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6003435.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8818..9180)
                     /locus_tag="OO335_003157"
     CDS             complement(8818..9180)
                     /locus_tag="OO335_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6003436.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9717..11630
                     /gene="mtlA"
                     /locus_tag="OO335_003158"
     CDS             9717..11630
                     /gene="mtlA"
                     /locus_tag="OO335_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6003437.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11725..12873
                     /gene="mtlD"
                     /locus_tag="OO335_003159"
     CDS             11725..12873
                     /gene="mtlD"
                     /locus_tag="OO335_003159"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6003438.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12873..13460
                     /gene="mtlR"
                     /locus_tag="OO335_003160"
     CDS             12873..13460
                     /gene="mtlR"
                     /locus_tag="OO335_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6003439.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13472..13681)
                     /locus_tag="OO335_003161"
     CDS             complement(13472..13681)
                     /locus_tag="OO335_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003440.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
BASE COUNT         3355 a         3706 c         3475 g         3304 t
ORIGIN      
        1 aatggcgtcg gcatgaggat gattacgttt tttagcaatc aggtagggaa tatcatgtaa
       61 aataataatc ccatagaata tcaccagaaa tacaaaaata agcactccca gtgcaaaata
      121 gtccaggaac atatttccct caaagaatat aaaaaagaac aattaacgca tattatgaaa
      181 tgccatgatg cagtgcaatt tccttattta tataacgaca tattgttttt tatatacttt
      241 ctgaaatgtg ctgtctggtt tttcaacagg acgaggttta tcatattgaa atagtggaac
      301 tattggccaa actaatgaat aactccagta aaacatccac agtacagatt aagcgtatta
      361 aaccttcaat tatctaccgt ttattgctga ttggcctcgg atcaccaatg gtgatttacg
      421 gcctggttcg cccgctcacc atcgaaacgc gagattaaac tcctgacaaa acacgcccca
      481 gaaacgcact tctgttggcg tagtctgggt tattgcgcac gtaggtttaa aacgtaaatt
      541 gtaacaacgt gcgctttgtt tatgccggat gcggcgtgta cgccttatcc ggcctacggt
      601 caggttcccg taggcatgat aagacgcgta agcgtcgcat caggcaatga atacccaatg
      661 cgaccagctt cttatatcag aacagcccca acggtttatc cgagtagctc accagcaggc
      721 acttggtttg ctggtaatgc tccagcatca tcttgtgggt ttcgcgaccg atacctgatt
      781 gtttgtagcc accaaacgca gcatgtgccg ggtaagcgtg ataacagttg gtccacacgc
      841 gcccagcctg tatgccgcgc cccatcttat aggccagatt accgttgcgg ctccagacgc
      901 ccgcgcccag gccatattgc gtatcgttcg ccagctccag cgcttcttcc atcgttttga
      961 aggtggtcac cgccagcacc gggccaaaaa tctcctcctg gaacacccgc atattgttct
     1021 gaccaaacag aatcgtcggt tcgaggtagt agccgtcttt cagttcacct tccagcagct
     1081 tgcgccgccc gcctgtgagc acgtcagcgc cctctttttt accgatatca atgtagttga
     1141 ggatggtttc cagttgcccg tgagaaacct gcgcgcccat ttgcgtcacg ctgtcgagcg
     1201 ggttaccgct gcgaatgctt tcgacacggc ggatggcgcg ttccataaag cgttcgtaga
     1261 ttgactcctg cactaaggca cgactcggac aggtgcaaac ttcgccctgg ttaaaggcaa
     1321 acagtgcaaa gccttccagc gctttatcga aaaaggcatc ttcttcatcc atcacatcag
     1381 caaagaagat atttggcgac ttaccgccca actccagcgt caccggaata atgttttgcg
     1441 ttgcgtattg cataatttgt tggcccactt ccgttgagcc ggtaaacgcc actttggcga
     1501 tgcgtttcga ggtcgccaga tattcgccaa tttccccacc tgcgccattg accacgttca
     1561 ccacgcccgg cggcagtaaa tcaccgacaa tttccattag cagcagtaca gaaagcgggg
     1621 taagacgtgc gggtttcagc accacacagt tgcccgccgc cagcgcggga gccattttcc
     1681 agctcgccat cagcagcggg aagttccacg ggataatctg ccccaccacg cctaacggtt
     1741 catggaaatg ataggccacg gtttcgctat caacttcact gatcccacct tcctgcgccc
     1801 gaatacacga ggcgaaatag cggaaatggt caatcgccag cggtacatcc gcagcactgg
     1861 tttcgcgaat gggtttgccg ttatcccagg tttcagctgt cgctaacagc tcgaggtttt
     1921 gttccattcg atcggcaatc ttaaacagaa tcgccgcgcg atcctgcacc gaggtgtgcg
     1981 cccatttatc tttcactttg tgcgcagcat ccagcgccag atcgatgtct cgtttgcccg
     2041 aagacgccac ttcgcacagc agctgcccgg tcaccggcgt cagattctgg taatactcac
     2101 catcggcagg ggctacccat tcaccgccaa taaagttgtc gtagcgggct tttaacttga
     2161 tggggaaacc atactcgccg ggcttaatct gtgctgaagg gggattattg gtcatgatcg
     2221 tctccttgcg gtgtgaggta taacaagggt agacgtgact ggcgaaatct tcgccagtcg
     2281 gtaacagctt tacgacagct atcacgaatt tacgggcaag gattacagag atagttgaaa
     2341 aagcggctga caatttgcca gccgttgttg gaatgatgat tacattgcgg cgcgataaat
     2401 caccacaatt tcttcgtgag ttgcctggat cgggttagta aagccacagg catctttcag
     2461 ggcattagtc gccagaaccg cgaaatcttc ttctttcacg ttcaggtcgc gtaggcctgc
     2521 cgggatatcc actttcttcg ccagttcacg gatggcgtta atgcaggctt cagcaccttc
     2581 cgcgtcgttt ttacctgtca cgttcacgcc cattgcagcg gcacagtcac gcagacgtgc
     2641 agcggcgact ttgctgttga atacctgaac atgcggcagc agaacggcgt tacatacacc
     2701 gtgtggcagg ttgtagaaac cgcccagctg gtgcgccatc gcatgaacat aacccagaga
     2761 agcattattg aacgccatac cggcaaggaa ctgggcataa gccattgctt cacgcgcttt
     2821 cgcattactg ccatcttcaa cggctaacgg caggttttcg gcaatcatgg tcacggcttt
     2881 cagtgcacaa gcgtcagtga tcggcgtggc ggcaatagaa acatacgctt cgatagcgtg
     2941 cgttaaggca tccatacccg ttgcggcggt cagtgacttc ggcataccaa tcatcagaga
     3001 ggagtcattg acagaaagca gcggagtcac gtgcttatca acaatcgcca ttttgttatg
     3061 acgcgcttcg tcagtgatga tgcagaaacg ggtcatttca gaggccgtac ccgccgtggt
     3121 attgatggcg atcatcggca gctgcggttt tgcagagcgg tcaacgcctt cgtaatcgcg
     3181 aatatcgccg ccattggctg ccaccagcgc aatacctttt gcgcagtcgt gtggagaacc
     3241 accgcctaag gagatcacgc tatcgcaatt attttcttta agtaatttca aacctgcggc
     3301 gacgttttcc gtggtggggt taggttgggt gccatcataa ataacgctaa aaatattgcg
     3361 ttcttccagt gctttttgca catcacccgc catacctaat ttcgttaaca tattgtcagt
     3421 gacaattaag gtacgggtaa atccataatc tgccatcata ttcattgcat cagtcaatga
     3481 atcagcgccg atgacattca cagaaggaat aaagaaagtt gaagatgcca taacacactc
     3541 ctgaaagtgt aaaaaagggt gggcagctta ctgaggattt tcattcgaaa acatgatcat
     3601 tattattaat tatcaaaatt aatctaataa aaagcacatt gtattcatta aacaatgtgc
     3661 tttatttaaa atcataagaa atttgtatta atttatttta aaaattaatt aatcatttcc
     3721 ttatttttcc ggaaataata atgcgtcgcg caataaatga tcatttccac gacgacgcgt
     3781 aaagccaatg cggtcaaaat attccagaat ctgaattgcc agctttcggc ctacgcctaa
     3841 gcgatcgcgg aaatccgccg cgcaggttga accacactcc tgatcgagat cgcggatcat
     3901 attggcaaat tcgacaatcc gatcgttacg gtaataacga tctttgacga tcgcggtaat
     3961 tattccttgc tgcgccgcct ggcgtagcgt caggcgcatt gcctgctcgt cggtacccgt
     4021 ctcttttgcc agatcacgca cccaccacgg ctcgtcacca aacagcggct ctgctttttg
     4081 ccagatggcc tgctgctctt cgctgaagcc cgctttgtga tccggcagat gcagccaacc
     4141 gtgatgactg aggatgtcgc cgctttcgcg catcttttca atcagcaaca gtactagcgc
     4201 ttcatcttcc attggcaacg ccatacgtcg cagacgttcg cgcccaggcc caggttcatc
     4261 gcgatgttgc tcatgataag tcgctaatgt gtcgagaatt ttccgttgcc agcgagcggc
     4321 aaccggcgca ttcaacaagc tataaccagc ctgaatatag ccaggctgtt gcagcagttc
     4381 gcgcatccct tcgccgttga gctggcgcgc ccaagcgaaa tcaggaagat taaccgcgcc
     4441 gcgttgcagg tgaacggcta acgcttcgct atcattctgt gccctggcga gggaagccag
     4501 ccattgcaga tactctggtt tacgtttacc gcgacgcggc gggttaagca tcacgacgcg
     4561 cgctccggcc agcgtgttgc gggcagagat atcgcgcaat accaggcggt cgttatctgc
     4621 cagccataac ggggtgtcga agaccagttc agcaaggtta tcttccagca gtgaaacgcg
     4681 tcccgtgacg tggctggcgg cgtggtgaat atgcagcggc tgccactggg tcagcggtgc
     4741 atgggtttga agctcgacaa tcacccgtgt gaacggctct ggcggcacat cggcaagcag
     4801 ccagtcgcca cggttaatct gctctttttc cgcatcaccc gcgatgtcaa gcgcgatacg
     4861 ctgcccggca ttggcggttt ctgttggctg gttttgcgca tgcagcgcac gtacacgcat
     4921 cggtttattt acaccagtca gccagagtga atcgcctacc ttcacttccc cgcttaacgc
     4981 cgtaccggtg acgaccagcc cggcaccttt tacggtaaat gcgcggtcaa tcgcgaggcg
     5041 gaaactatgt tggctggcgt gctcgcgttc cggcaactga agcagatgct cgcgcagggc
     5101 atcaattccc cgaccttcgg ttgctgcggt gataaacagt tttgcctcag caaaaccgta
     5161 ttcccgcaga acctccttta cctggcgttc aacctcatca acacgcgctt cgtccacgcg
     5221 atcggctttg gtcagcgcca ctgtcagcat cgggttaccg gtcagctgca aaatcgccag
     5281 atgctcacgg gtctgtgcca tcacgccgtc atcgcacgcc accaccaaca gcgcgtgatc
     5341 gataccacca acgcccgcca gcatgttgga aagaaacttt tcatgaccgg gaacgtcgat
     5401 aaaaccaggc acgcgaccat ccggctgcgg ccagtaggca tagccgagat cgatggtcat
     5461 gccgcgcttt ttttcttccg gcagacggtc agcatttacg ccagtaatcg cctgcaataa
     5521 ggttgttttg ccgtggtcaa cgtgtccggc agtcgcaata atcatttcaa caacatctcc
     5581 aaaaaccgtt gctcatcttc aaggcagcgt aaatccagcc acaatcgtcc gtcataaata
     5641 cgaccaatca ccggcactgg caattcacgc cagcgggcgg ctaatgactc aaggtggcta
     5701 ccgcgtccat catggggtgt aaacgttaat gccgcgctcg gcaggcgatc aaccggcagc
     5761 gaaccactgc caatctgcga aagacatggc ataacctgta ccgcaaactc cgcgccgtaa
     5821 tgtgcgacaa ggggagcctg taaacgttgt gcctggattt gaatgacctc tgcgctgcgg
     5881 gtaagcaggc gcagggtcgg taatttttca ctcagagctt cagggtgtaa ataaagacgc
     5941 aacgtggctt ccagcgccgc gagggtcatt ttatccgcgc gtaatgcacg cttcagcggg
     6001 tggctttgca ggcgggcgat catctctttt ttaccaacga taatcccggc ctgcggcccg
     6061 cctaacaact tgtcgccgga gaagctcacc agactgacgc ccgccgcaat caactcctgc
     6121 ggcattggct ctttcggcaa accgtactgg ctaagatcga ccagcgagcc actgcctaaa
     6181 tcagtcacca ctggaacatc cagctctttg ccgagcgcca ccagttcagc ttcgtctatc
     6241 gctttggtga acccctgaat gctgtagtta ctggtatgta ctttcatcaa cagtgcggta
     6301 ttttcattca ccgcctgacg ataatcattc gcgtgcgtgc ggttggtggt ccctacttcg
     6361 tgtagggtgc agcctgcctg acgcataaca tcgggaatac gaaacgcgcc gccaatctcc
     6421 accagttcgc cgcgagatac caccacctct ttcccgctgg cagtggccgc caacatcaat
     6481 aacaccgccg ccgcattgtt attgacgata caggcatctt ccgcccccgt aatacggcac
     6541 agcagctgcg ccagcgcccg atcgcgatgt ccgcgtccgg cgtcgtccag atcgtactcg
     6601 agggtcactg gcgaacgcat agcctgcgca acggcttcca ccgcggcttc cgcctgtaaa
     6661 gctcgcccaa ggttggtatg cagcacggtt cccgtcaggt tgatcaccgg acgcagcgcg
     6721 ctctgcgcct ctttcgtcaa ccgggcatcg acttcttgcg cccagttttc acaccacgca
     6781 ggcagcgtct ggctgccacg aatcacttct cgcgcttcgt cgagcatctg acgcaacaat
     6841 tccaccacgc gggtgtgacc ataagtatca cgcaaagaaa ggaaggagct atcgcgcaat
     6901 aagcgatcaa tagccggaag ttgactatag agggaacgcg tttcggttgt cataggaaac
     6961 ctggctgatc aaggccctct cacacggaga agggcgttta acataaccac ggattgtaac
     7021 gtgagatggg tcaggaggac atatcgcgca tcaagccttt ggcggttcgg tgcgagcaaa
     7081 actttcgcgg ctaaacaggt tttcgaccag tttgactaag tgtgggcggt cgacacacca
     7141 acctggcgca acgcggcgga aattgagata tccgacagca caggcaatgg cgatagtcgc
     7201 cagattgacc gtatcggttt tgagtgtgcc atcgaccaga tatccttcca gcacatccag
     7261 actgcggttg attttctccc gctggcgtaa caattcatct tcagactgct gcgccgccgg
     7321 acgcgcctgt tcacgcaccg ataccagccc ggcatccata atgccatccg ccagcgcctc
     7381 aattttgcgc acccgcaacg actccagcgg atcgcgcggc aacatcgccg gagcgacatt
     7441 cattaactca atatattcag cgatgatcgg cgaatcaaac cagcattcgc cctcttcggt
     7501 caccagcacc ggcactttcc ctaacgggtt aaattgcgcc acgccgttat ccgcgttata
     7561 gggcagttca ttaataaatt cgaaagttat gcccttttct aacaacagaa taaaaagttt
     7621 gcgtacaaac gggctggtgt agctaccgac gagtttcatg ccgagtcctt tgtgcgagga
     7681 aaaatatcag tatggctcag gctcacgcaa aacacaggtg atataagtct caatgatcca
     7741 aataaagata atgcatccaa ctggtcattc ttagcagcac tttccgcatc accgaaacat
     7801 ggctgaaatg gtcggagtaa accgccacct gggcgtagat gccgtcgggt aaggcatcga
     7861 tatcatcgtt agggtccagt tcaatggttc ccagcacacc gtccgtgccg ggaacgaccg
     7921 ttaatgattg caataccccc tgcgcctgat aagaaccgcc tggcacgaca ggtaaaatac
     7981 tagtcaattt gccgtggaac acctgcccag gtagcgcgtt aaacaccact tccgcatcat
     8041 cgccaggttt cagacgtaac agcgagtttt gccgaaattg ggcgacaatt tgccgttttt
     8101 gctcggggat gaagaccatc accggacgca gcggcaaggc agctgcgtat gtacctgggc
     8161 ggatcagtac ctgagtgacg tagccattgc ttggtgcgcg aatgacagtc tgctcaagat
     8221 tatattttgc ttcagtaagt tgcgctctta agctcacaat ctgcgattgc tcgccgttga
     8281 ccatactgtc gagctgactc tggatctgcg cctgctccgc caccgagcct ttcactaacg
     8341 catcctgcgc gaggaaattt tgccgcgcat cgtcgatgtc acgttccgag aacggattca
     8401 ccgccgcctg gctgcctttc aggtaacgtt gataattttt aaacagacgg tcgcgctccg
     8461 ctgaaacctg ggtggtgttg gcctgcgctt cagtgagctg cgcacgcagc gtctttatat
     8521 tatgcgtcgc cgtcatcagg tcagcctgaa gtctgtcaac tcgcgcctgg taacgaaccg
     8581 ggtcgagctt aaaaagcacc tcgccctttt gaataagctg attattcttg tcagtgactt
     8641 cagtaacaat tcccgtcacc tgtggcgtga tagggatcgc tatcactgcc ttttgcgcgg
     8701 taaaagtgta agggtggttg tagttcatca acaaaatcaa accactcacc agaaacacgc
     8761 ctcccagcgc cgccgtcgcc agcgccacgt aagttaaaac aatcaataga tccataatta
     8821 gatctccggg aacgtggaat agtcagtctt tttctcggcg gcgagttgat gctccagccg
     8881 ggaaattcgg tcagaaagtg cggcgatttc cggttcagtc gctttctcct gcgacgcaac
     8941 atgtgactgc ataccccagc cacgctccgg ttgatagagc gtcgcccaga tccacagaaa
     9001 cggccagata acatgcagag taaacaggct cacccagccc gccgtatgaa tagcgtcggc
     9061 atgggggtga ttgcgctttt tggcaatcag ataagggatg tcatgtatcg cgatgattcc
     9121 ataaaaaatc accagaaaga cgaagatcag cactcccaac gcgaaatagt ttagaaacat
     9181 atctgcctcg gattcacgtt tatcagtgtt gttttttggc tggcagccag aagggagtca
     9241 ggctgatatt ttgacaataa tccgggttcg cgattctcgc cataacacca aagaataatt
     9301 tttagaggtg atgagttgct tagttacata acgattgtat gacgaaggca taacatgctg
     9361 tagatcacat caggtgaacg ccgtaagaaa atatcttgtg attcagatca caaagattca
     9421 acaaaccatc aaaacaaaaa tgtgacatta ctcacattta aatgccgttt ttagcgaaaa
     9481 tcaccgcctt gtttcttttt tatacaggcg ttttgtgatg agcgtcacgt caattacctc
     9541 tctaccccct atatttatgt gactgatatc acacaaaagc ccgtcgactg gacagttaac
     9601 cgattcagtg ccagatttcg cagtatctac aaggtccggc tacctctgcc gccacattaa
     9661 caaaaaacct cgggcttcca gcctgcgcga cagcaaacat aagaaggggt gtttttatgt
     9721 catccgatat taagatcaaa gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc
     9781 caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc gttatttatt ccaacagggt
     9841 ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat gatcacttat ctcctaccgc
     9901 tgctgatcgg ttataccggt ggtaagctgg taggcggcaa acgtggtggc gtagtcggtg
     9961 ccattaccac catgggcgtt atcgtcggcg cagacatgcc gatgttcctc ggttctatga
    10021 ttgcaggtcc gctgggcggc tggtgcatta agcacttcga ccgctgggta gacggtaaga
    10081 tcaaatccgg ttttgagatg ctggtgaata acttctccgc aggcatcatc gggatgatcc
    10141 tcgctattct ggcattcctc ggcattggcc cgattgttga agccctgtcc aaaatgctgg
    10201 ctgcgggcgt taacttcatg gttgtccatg acatgctgcc gctggcgtct atctttgttg
    10261 aaccggcgaa aatcctgttc ctcaacaacg ccattaacca cggtatcttc tcgccgctgg
    10321 gtattcagca gtcccatgaa ctgggtaaat caatcttctt cctgattgaa gctaacccag
    10381 gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt
    10441 ctgcgggcgg tgcggcaatc atccacttcc tgggtggtat ccacgaaatc tacttcccgt
    10501 atgtgctgat gaatccgcgt ctgatcctcg cagtcatcct cggcggtatg actggcgtgt
    10561 tcacgctgac tatcctgggc ggtggtctgg tttctccggc atctccgggt tctatccttg
    10621 ctgtactggc gatgacacca aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg
    10681 cgatggctgt ctccttcgtt gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag
    10741 aagatgatat tgaagcagca actcgtcgta tgcaggacat gaaagctgag tctaaaggca
    10801 catctccgct atctgcgggc gatgtgacta acgacctgag ccacgtacgt aaaatcatcg
    10861 ttgcctgtga cgccggtatg ggttcaagtg cgatgggcgc aggcgtgctg cgtaagaaaa
    10921 ttcaggatgc aggtctgtcg cagatttcgg tcactaacag cgcgatcaac aacctgccgc
    10981 cagatgtgga cctcgtcatc actcaccgtg acctgaccga acgcgctatg cgccaggttc
    11041 cgcaggcaca gcatatttcg ctgaccaact tcctcgacag cggcctgtac accagcctaa
    11101 ccgaacgtct cgttgctgcc cagcgccata ctgaaaacga agtgaaagtg aaagacagtc
    11161 tgaaagacag ctttgacgat tccagtgcta acctgttcaa actgggcgcg gagaacatct
    11221 tcctcggtcg caaagcagca accaaagaag aagcgattcg ttttgctggc gagcagctgg
    11281 tgaaaggcgg ttacgttgag ccggaatacg ttcaggcgat gctggatcgt gaaaaactga
    11341 ccccgactta tctgggtgag tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc
    11401 gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag
    11461 aagaagatga cattgcccgt ctggtgattg gtattgcagc ccgtaacaac gagcacattc
    11521 aggttatcac cagcctgacc aatgcactgg atgatgagtc cgtcatcgag cgtctggcac
    11581 acaccaccag cgtggatgaa gtgctggaac tgctggcagg tcgtaagtaa tccaatccta
    11641 ccctctcctc acggagaggg tttgggtgag ggaaaagcct caccccagcc ctctcgggta
    11701 aaaacattga tgaaggttaa tactatgaaa gcattacatt ttggcgcagg taatatcggt
    11761 cgtggcttta tcggtaaact gctggcagac gcgggtatcc aactgacgtt tgccgatgtc
    11821 aatcaggtgg tacttgatgc cctgaatgcc cgtcatagct atcaggttca tgtggtcggt
    11881 gaaaccgagc aggtagatac cgtttccggc gtcaatgctg tcagcagcat tggtgatgat
    11941 gtcgttgatc tgattgctca ggtcgattta gtcactaccg ccgttggccc ggttgtgctg
    12001 gaacgtattg ctccggctat cgccaaaggg ctggtgaaac gcaaagaaca aggtaatgaa
    12061 tccccgctga acatcatcgc ctgtgaaaac atggtacgcg gcaccacgca gctgaaaggt
    12121 catgtgatga acgccctgcc ggaagacgcc aaagcgtggg tagaagagca cgttggcttt
    12181 gtcgattccg ccgttgaccg catcgtaccg ccttcggctt cggcaactaa cgatccgctg
    12241 gaagtgacgg tagaaacttt cagcgaatgg attgtcgata aaacgcagtt caaaggcgca
    12301 ctgccgaaca tcccaggcat ggagttaacc gacaacctga tggcatttgt cgaacgtaaa
    12361 ctcttcaccc tgaacacggg tcatgctata accgcgtacc tcggaaaact ggccggtcat
    12421 cagaccattc gtgacgctat tctcgacgag aaaatccgcg cggtggtaaa aggtgcgatg
    12481 gaagaaagtg gtgcggtact gatcaagcgc tacggctttg acgcagacaa acatgcggcg
    12541 tacatccaga aaatcctcgg tcgttttgag aacccgtatc tgaaagatga tgtagagcgc
    12601 gtaggccgtc agccgctgcg taaactgagt gctggcgacc gtctgatcaa gccactgctc
    12661 ggtacgctgg aatacagcct tccgcacaag aatctgattc aggggattgc tggtgcaatg
    12721 cacttccgca gtgaagatga tccgcaggct caggaactgg cagcactgat cgctgacaaa
    12781 ggtccgcagg cggcgctggc acagatttcc ggtcttgatg ccaacagcga ggttgtatcc
    12841 gaggcggtaa ccgcttataa agcaatgcaa taatggtgga ccaggcgcag gacaccctgc
    12901 gcccgaataa cagattgtca gatatgcagg caacaatgga acaaacccag gccgttgaaa
    12961 accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg aagctttctg atcaccgccg
    13021 tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc aaagacgatt
    13081 acgcggtgaa gtatgctgta gaaccgttac tcgacggcga cggtccgctg ggcgatcttt
    13141 ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg
    13201 cggaactgct gatggcattg cgtgaagagc taaatcacga cggcaacgag tacgccttta
    13261 ccgacgacga aatccttgga ccctttggtg aactgcactg cgtggcggcg ttaccaccgc
    13321 cgccacagtt tgaaccagca gactccagtt tgtatgcaat gcaaattcag cgctatcaac
    13381 aggctgtgcg atcaacaatg gtcctttcac tgactgagct gatttccaaa atcagcttaa
    13441 aaaaagcctt tcaaaagtaa gcaacgtctg cttactgccc ctctacctgc ttcggccgat
    13501 aaagccgacg ataatactcc agacgttgta gatataacgg cacgctttca tcgggtattc
    13561 cggacggaat cgcgttacgg ggaggaagtt ttttcagata ctcccggaac gcctggcttg
    13621 atgccatgaa atctacggct ttatcgataa gaagcggaac gttttcacct aatttgccca
    13681 tgatgatatc tccgtattac cccgcgccgg gaatgcgcgg ccgccaattt tagtttaggt
    13741 tcccggtaaa gatgacatta aggaaaacgt gctgaatcct caataatcat ccgctgctgt
    13801 ttattatctt tttctataga tttcttaatt aatcaacgaa
//
LOCUS       DAJTSF010000098        13248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000098 DAJTSF010000000
VERSION     DAJTSF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.098"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(16..998)
                     /gene="kdgT"
                     /locus_tag="OO335_003162"
                     /pseudo
     CDS             complement(16..998)
                     /gene="kdgT"
                     /locus_tag="OO335_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="OO335_003163"
     CDS             complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="OO335_003163"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6003441.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2163..3197
                     /gene="rhaT"
                     /locus_tag="OO335_003164"
                     /pseudo
     CDS             2163..3197
                     /gene="rhaT"
                     /locus_tag="OO335_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3194..4060)
                     /gene="rhaR"
                     /locus_tag="OO335_003165"
     CDS             complement(3194..4060)
                     /gene="rhaR"
                     /locus_tag="OO335_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR6003442.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="OO335_003166"
     CDS             complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="OO335_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6003443.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5240..6709
                     /gene="rhaB"
                     /locus_tag="OO335_003167"
     CDS             5240..6709
                     /gene="rhaB"
                     /locus_tag="OO335_003167"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6003444.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6706..7965
                     /gene="rhaA"
                     /locus_tag="OO335_003168"
     CDS             6706..7965
                     /gene="rhaA"
                     /locus_tag="OO335_003168"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6003445.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8207..9031
                     /gene="rhaD"
                     /locus_tag="OO335_003169"
                     /pseudo
     CDS             8207..9031
                     /gene="rhaD"
                     /locus_tag="OO335_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9041..9355
                     /gene="rhaM"
                     /locus_tag="OO335_003170"
     CDS             9041..9355
                     /gene="rhaM"
                     /locus_tag="OO335_003170"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6003446.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9656..10102
                     /locus_tag="OO335_003171"
     CDS             9656..10102
                     /locus_tag="OO335_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR6003447.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10113..11564
                     /locus_tag="OO335_003172"
     CDS             10113..11564
                     /locus_tag="OO335_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR6003448.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRPGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11554..12624
                     /locus_tag="OO335_003173"
     CDS             11554..12624
                     /locus_tag="OO335_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6003449.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12624..>13248
                     /locus_tag="OO335_003174"
     CDS             12624..>13248
                     /locus_tag="OO335_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR6003450.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3158 a         3459 c         3491 g         3140 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc cagcttcttc tttcgtaccg
      601 tactgctgca tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc
      661 gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc
      721 gaggcaattg ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat
      781 ttacgcagta ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac
      841 accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc
      901 cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc
      961 ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt
     1021 tttaagggct gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga
     1081 cattcattta atcaataaaa gtgatcaaaa tcacatcaat tgaaacgctg ttttattttt
     1141 ataggaccat tgtttttatt ttggaattga tcacaaaaaa accaccgccg ttggcgatgg
     1201 ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg caaatgatta
     1261 ttttttcgcc gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt
     1321 gatgtagtcc ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag
     1381 gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg
     1441 gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga
     1501 accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa
     1561 gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt
     1621 ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc
     1681 tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg
     1741 caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg
     1801 gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga
     1861 tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc
     1921 gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat caatgccgta
     1981 cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact
     2041 taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag
     2101 cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga
     2161 agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag
     2221 cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag
     2281 tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg
     2341 atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg
     2401 gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt
     2461 cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa
     2521 ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg
     2581 gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc
     2641 gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga
     2701 tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg
     2761 aagccgctgc cgcacttggc gtcgatccac tgtatgttgc tctgccaagc tatgttgtca
     2821 tcatgggcgg cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga
     2881 aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt
     2941 tactctcggc actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc
     3001 acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg
     3061 tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc
     3121 cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca
     3181 tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc
     3241 cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg
     3301 aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga
     3361 cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa
     3421 cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc
     3481 tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg
     3541 aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag
     3601 ccatttcgtt agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct
     3661 gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc
     3721 taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa
     3781 taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa
     3841 tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat
     3901 taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt
     3961 gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga
     4021 gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg
     4081 caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc
     4141 tggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta
     4201 ctgtcgctga atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagt
     4261 agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt
     4321 tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca
     4381 tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt
     4441 gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc
     4501 ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc
     4561 atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac
     4621 ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat
     4681 cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga
     4741 tgccgatcat gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca
     4801 ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga
     4861 tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac
     4921 ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc
     4981 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
     5041 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
     5101 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
     5161 ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga
     5221 aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc
     5281 cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga
     5341 aatccatcgt tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga
     5401 tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat
     5461 cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca
     5521 gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc
     5581 acaacagcaa ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt
     5641 caatacgctt tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat
     5701 tgctcacgct ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg
     5761 ggaatatacc aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga
     5821 gtcgctactg gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg
     5881 taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc
     5941 cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta
     6001 tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga
     6061 cacggcgctg gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct
     6121 gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga
     6181 tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc
     6241 caatgacgat cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg
     6301 ggaaacggcg caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag
     6361 tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt
     6421 ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc
     6481 cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat
     6541 cggcatcgag ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt
     6601 cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta
     6661 tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg
     6721 gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag
     6781 gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt
     6841 tccggttttg aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat
     6901 ccgggcaaag cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg
     6961 attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc
     7021 tcgcgcgacc agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat
     7081 cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt
     7141 acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc
     7201 cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg
     7261 atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg
     7321 gcagcactgg atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt
     7381 gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac
     7441 atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg
     7501 actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtaccgca gttgctgctg
     7561 cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc
     7621 caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt
     7681 gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg
     7741 aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg
     7801 gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgcggcag
     7861 gcggtctggg aaatgtattg ccaacatcac gatacgccag caggtagcga atggctggag
     7921 agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg
     7981 gcacgagcgc cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc
     8041 ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa
     8101 caccgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc
     8161 cgcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac
     8221 ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctagc tgaaaggctg
     8281 ggacgagcgc aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta
     8341 tcacgacaac ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact
     8401 ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga
     8461 tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct
     8521 ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca
     8581 ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac
     8641 caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact
     8701 gtgggaaggc agcaccgaat gtctggtagt attcccggat ggcgttggca ttctgccgtg
     8761 gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc
     8821 gctggtgttg tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt
     8881 tggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg
     8941 cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt
     9001 tacaccacta gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt
     9061 catgcaggta aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc
     9121 ggaactggaa gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa
     9181 agcgcgtaat ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt
     9241 agccagtact gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa
     9301 cccagataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc
     9361 caatgtgccg gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat
     9421 attacatctc ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat
     9481 gaataaccca ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc
     9541 aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc
     9601 acttaatcgc tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc
     9661 agctcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct
     9721 gaagcagttg gcgacaatag cgttacaaaa cggttttatc accgactcac accagttcct
     9781 gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg gtgtcgcagt
     9841 gccgcacggt aaaagcgcct gcgttaaaca accgttcgta ttattcgccc gcaaagcgca
     9901 ggctattgac tggaaagcca gcgatggcga agacgtcaat tgctggatct gcctcggcgt
     9961 gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc gcaaaattat
    10021 tcaccaggaa tttattcatc aactgcaaca gggcgatacc gaccaggtgc tggccttgtt
    10081 aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc atccttacgt attgtcgcga
    10141 tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc ctggaacaga
    10201 aagcccgttc tctcggccat accataaaag tggaaactca agggtccagt ggcgttgaaa
    10261 accgcttatc cagcgaagag attgccgctg ccgattacgt cattcttgct accgggcgtg
    10321 gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa agtttatgag attgccatct
    10381 ccctggcgtt gaaaaatatc gaccagattt tcagcgaatt accgacaaac tcgcagcttt
    10441 ttgccgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc agcgtgatga
    10501 gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg
    10561 tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg
    10621 cgccgtcgtt tacctgggta gttgaatcca tcggttacct tggcttcacc tttatgatcc
    10681 ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc gctccggcgt
    10741 ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat
    10801 tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg ttccgcaaag
    10861 tgcgccctgg caaagcatta caaccgctgc tcggttcaat gctgatcccg ttcgttactc
    10921 tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc gacctcatgg
    10981 gcgggctgct ccacttcctg aataccattc ctccatcaat gaagtttgcg gcagcgtttc
    11041 tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc gcctggttct
    11101 tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc ggcgttgtcg
    11161 cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc
    11221 gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac
    11281 cggctatacc ttacgctctg gcagctccgt tgccgatgat taccgccaat acgctggcgg
    11341 gcggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca cctggtttgg
    11401 gtatctttga cccgctgatc ggcctgatgt cgccggtagg ttcgttttat ctggtgctgg
    11461 cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggattg tggttacgcc
    11521 gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact gcaacagttg
    11581 tgcgaagcca gcgccgttag cggcgatgaa caggaagttc gcgacatcct gataaacacg
    11641 ctggaacctt gcgttaatga aatcaccttt gatggtctgg gcagctttgt tgcccgtaag
    11701 ggaaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc
    11761 acccacatcg acgagagcgg ttttctgcgt ttcaccacca ttggtggctg gtggaatcag
    11821 tcgatgctca accatcgggt aaccatacgc acacacaagg gagtgaaaat ccctggtgtg
    11881 attggtttcg tcgcgcctca tgcgttaacg gaaaagcaaa ggcaacagcc gctgtcattt
    11941 gatgagatgt tcattgatat tggcgcaaac agtcgcgatg aggtggaaaa acgcggtgtg
    12001 gcgatgggcg attttattag cccggaagcc aattttgcct gctggggcga agataaagtg
    12061 gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga tggctgaact attgcagacg
    12121 gtgaataatc ccgaaattac gctgtatggc gtcggcagcg tggaagaaga agttgggcta
    12181 cgcggggcac aaacctcggc ggaacacatt aaaccggatg tagtgatcgt gctggatacc
    12241 gctgtcgcag gcgatgttcc gggcattgat aacattaaat acccgctgaa actgggccag
    12301 gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact ggtagcagcg
    12361 ttaaaaaact gtgccgcaca taacgattta ccgctgcaat tttccaccat gaaaaccgga
    12421 gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt tgcgctgtgc
    12481 ctgccaaccc gctatctgca cgctaacagc ggcatgattt caaaagccga ttacgatgct
    12541 ctgctcacgt tgatacgggg ttttctgacg accttaaccg cggagaaagt caacgcgttt
    12601 agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc
    12661 tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt tcaggcagga
    12721 ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc cgcattttcg
    12781 ccagtggcag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc
    12841 tgcaaaagca cgataacgac gatcggctgc tggcgctgtt attactgaat actttcactc
    12901 cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac gtgggtagca gagcgtctgc
    12961 cccagttaaa acagcgttat gaacgcactt gttgcctggc cagccgccct ggtttgggcc
    13021 atttcattga tgagacagaa gagaaactcg ttatcttgct ggcgaacttg ctgcgcaaag
    13081 atccgttttt aattccgctg gcgggcataa cacgagacaa ccttcagcat ttatccacgg
    13141 cctgcgacaa ccaacaccgc tggccgctca tacagggtga ttatctctcc agcctgattc
    13201 tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaat
//
LOCUS       DAJTSF010000099        13175 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000099 DAJTSF010000000
VERSION     DAJTSF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13175
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.099"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(160..480)
                     /locus_tag="OO335_003175"
                     /pseudo
     CDS             complement(160..480)
                     /locus_tag="OO335_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
     gene            complement(686..1405)
                     /locus_tag="OO335_003176"
                     /pseudo
     CDS             complement(686..1405)
                     /locus_tag="OO335_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1421..1753)
                     /gene="cusF"
                     /locus_tag="OO335_003177"
     CDS             complement(1421..1753)
                     /gene="cusF"
                     /locus_tag="OO335_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR6003451.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1777..3159)
                     /gene="cusC"
                     /locus_tag="OO335_003178"
     CDS             complement(1777..3159)
                     /gene="cusC"
                     /locus_tag="OO335_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR6003452.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            3316..3999
                     /gene="cusR"
                     /locus_tag="OO335_003179"
     CDS             3316..3999
                     /gene="cusR"
                     /locus_tag="OO335_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR6003453.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3989..5431
                     /gene="cusS"
                     /locus_tag="OO335_003180"
     CDS             3989..5431
                     /gene="cusS"
                     /locus_tag="OO335_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR6003454.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVAPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5581..7818
                     /gene="nrfB"
                     /locus_tag="OO335_003181"
     CDS             5581..7818
                     /gene="nrfB"
                     /locus_tag="OO335_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR6003455.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7805..10773
                     /gene="nfrA"
                     /locus_tag="OO335_003182"
                     /pseudo
     CDS             7805..10773
                     /gene="nfrA"
                     /locus_tag="OO335_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10774..11664
                     /locus_tag="OO335_003183"
     CDS             10774..11664
                     /locus_tag="OO335_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR6003456.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11847..12608
                     /gene="envY"
                     /locus_tag="OO335_003184"
     CDS             11847..12608
                     /gene="envY"
                     /locus_tag="OO335_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR6003457.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12654..12730)
                     /locus_tag="OO335_003185"
     tRNA            complement(12654..12730)
                     /locus_tag="OO335_003185"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12694..12696),aa:Arg,seq:tct)
     gene            12973..>13175
                     /locus_tag="OO335_003186"
     CDS             12973..>13175
                     /locus_tag="OO335_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR6003458.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3230 a         3416 c         3348 g         3181 t
ORIGIN      
        1 acgtgggtgg actggtataa caatcgacga ttgctggaaa ggctgggcca catccctccg
       61 gcagaagcag aaaaagctta ttatgcttcc atcggaaacg atgatctggc agcctgagtt
      121 cacagataaa acactctcca ggaaacccgg ggcggttcag ttgtcgaacc agtagtcgag
      181 acactgggcg accgtcatgg agttctccac cttctcttca aaataagtgc gtggatccgt
      241 tcctgagaaa tagagctttc gcaagtcagc agtgatctgt ctggcatcct tcaaagacag
      301 ggatgggtat cgaccaagcc caagtcgatt aggcttgcca tgccagcgat agcggtactg
      361 gaactggatg acccccttcg gtgaaattcg tacgctgagg ccatcagcat cagccaattc
      421 ttgtgggcca gaatatggtt taccataaat agtacgcagt tttgtgtcgc ttatagccat
      481 aaataatatt tggtacgctc agaaataatg gtttggtaca catctggtac acaatatcac
      541 atggacaaca gcgcacagta aacaactata tttgacgttc gtagacatac tcaggtgaaa
      601 aaaaggttgt ttttcgagtt ataacagaca gttaattaac tacagtggac aatgctagac
      661 aatgacagac acagataagc aacctacctt cctctttcgc gaccacgtta tctttggcga
      721 tattcattcc cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggc gctttgagcg
      781 taaagcgagt ctggattttt tgcgtggcga tcaggtggcg aatatccgcc tccggcattc
      841 ccgccagtcg cagccgctca agaatgcctt cagtctgggt cgccgtaccg ccggtttcgc
      901 gcaacagtaa atactcactc tgcgcttcca cccagtcggg aatggtcagg tcgagaagcg
      961 gtgtgccctt ttgtacttta tcgcccacgg taagcggata caccttgtcg ataaacccgg
     1021 cagcgcgagc ctgcacaatg gcatactgat actcgttgta actgacattc gtcgggaaac
     1081 tctgggcaaa agtcagcggc ccgcgcgtga cggtagccgt tttcaccccc agattctgcg
     1141 tctgagtcgg gtcaatgcgc acaccagacg cagaactctc ttcatcggca tatttcggca
     1201 ccagatccat atccataaac ggcgatttac ctggtttatc gaaccgcgta ttgggataca
     1261 tcgggtcgta ccagaataag attttacgtt ctgcggtcgg cattttttct gcgtgcggtt
     1321 ccgcctggtc aaaccaggta aaacctgccg ctgaaataat accgcccgcg atcatgctgc
     1381 cgataataag cgcgattttt ttcatctcat taaacctggg ttactggctg actttaatat
     1441 cctgtaataa agaaaggttg ccctgctgga caaaattaaa cgccactttg tcgccggttt
     1501 taatttcact cattttcgtc tgcggggtga tggtaaagcg catggtcatc tccggccagt
     1561 tgacggcagc aatcggatcg tgatggatgg tgattttttt gctttccaga tcaacacctt
     1621 ttaccacacc ggtggcacta atgacctgtg gttgtgcttc gctcatggtt tcatgatgat
     1681 gttcgttagc ctgggcatta aagccaataa cggtaaacag actgaacatt gcgacttgca
     1741 gtgctttttt catttattct ctcctggagt taaaaattat tgctgccaac cgccccctaa
     1801 ggcggtatac aaataaattt cgttaacctg acgggcataa ttcaggtcaa gtaaagtttg
     1861 tcgggttgca aataaagaac gctcggcatc cagcacttcc agataactta ccgcgccgtg
     1921 ctgatataat gcccgcgccc gttgcagggt aatttgcagc gacgccagat aacgctgctg
     1981 ggcgctgatt tgatcgtcca ggctttgacg taatgccagc gcatctgcca cctctttaaa
     2041 ggcgttctgg attttctgtt cataattcac caccgactgc tgctggcgaa tttcggcgat
     2101 atccagattg gcctggttgc gtccggcatt aaaaatgggg atctcaattt taggaataaa
     2161 attccacatc ccgctgctag cgttaaataa tgacgacaga tcgctgctgg cggttgatat
     2221 tccgctggtc aggctgatag acgggaaaaa tgccgcacgc gcagcaccaa tattagcatt
     2281 agccgccatt aacgcgtgtt cagcttccat aatatccggg cgctgcaata agatttgcga
     2341 cgacaagccc gccggtaatt taacgctttg caggctgccg ctgtttactg tctgcgcttg
     2401 cggtagcttg ccgtagcttc ccaataacag ttgcaatgca ttattcgcct gcgccagttc
     2461 cccctgacgt ttagcgatgt cactgcgggt actttctatc accccgcgag cctgttccag
     2521 cgccagaaca ttgctgctac cggtcaacag ttgtttttcg acaaacgcat atgactgctg
     2581 ataattacgc agcgtttctt cggctatttg caattgcgca tacgccagct gctgattgaa
     2641 atagctttgc gcgacattag aaaccagcaa aatatgcacc gcgcgctggg cttcctcagt
     2701 ggctaaataa ttttggcgct cggcttcgct catgttcttt aagcgaccga aaaaatcgag
     2761 atcaaagctg gcgttaaggc cagtcgagaa ctcccgcgtc gtggctgaat cgcctttaag
     2821 attgccgctc cagctgccgc tgccctcgcc attgagctgt gggtagcggt cggcatcggt
     2881 cagacgatat tgcgcccgtg cctcctgcac tttcagcgtc gccatgcgca aatcccggtt
     2941 attcaccagc gcctcgctaa tcagcgtctt cacctgatta tcaacaaaaa aggtgcgcca
     3001 gcccacgttc tggtagttat ccgctgcgtt aaccaggccg ttctggctga gtgagaactg
     3061 ctgcggtacg ggcattaccg gacgctgata atccggtgcc agtgaacaac cagttagcgc
     3121 aagggccaca caaaatggca gaagtttaca aggagacata ggctcataat ttctggtgat
     3181 tttatgtcgc caactttact cgccaggctc tgattttccg gtgacaggaa aatgacaaaa
     3241 ttgtcatttt gccaataagc gattgccatc tgatcccgct actctagaat tgcccgggca
     3301 acatgcggag gaaatatgaa actgttgatt gtcgaagatg aaaagaaaac cggagaatac
     3361 ttgaccaaag ggttaaccga agccggtttt gtggtcgatt tggccgacaa cgggctgaat
     3421 ggctaccatc tggcgatgac cggtgattat gacctgataa tcctcgatat tatgctgcca
     3481 gacgtgaacg gctgggatat cgtgcgcatg ttgcgctccg ccaataaagg gatgccgatt
     3541 ctgttgctta ccgcgcttgg caccattgaa catcgcgtca aggggctgga gttgggggca
     3601 gatgactacc tggtgaaacc gttcgctttt gctgaactgc tggcgcgggt gcgcactctc
     3661 ctgcggcgcg gggcggcggt gattatcgaa agtcagtttc aggttgccga tctgatggtc
     3721 gatctcgtca gccgcaaagt cacccgcagc ggcacgcgca tcactctgac cagtaaagaa
     3781 tttactctgc tggagttttt tctccgccat cagggcgaag tgctgccccg ctcgcttatc
     3841 gcctcgcagg tatgggacat gaatttcgac agcgacacta acgccattga tgtggcggtg
     3901 aagctgctac gcggcaaaat cgacaacgac tttgagccga agctgattca aaccgtgcgc
     3961 ggcgtgggtt acatgcttga ggtgccggat ggtcagtaag ccatttcagc gcccgttttc
     4021 gctggcaacc cgcctgacct tttttatcag ccaggccacc atcgcggcgt ttttcgcctt
     4081 tgcatggatc ataatccact cagtaaaagt gcattttgcc gagcaggaca ttaatgattt
     4141 aaaagagatt agcgccaccc ttgaacgggt actaaatcac cctgacgaaa cgcaagcccg
     4201 acgcttaatg acgctggaag atatcgtcag tggttattcc aacgtgttga tttccctggc
     4261 agatagccac ggcaaaacgg tgtatcactc ccccggtgcg cctgatatcc gcgagtttgc
     4321 acgtgatgcc atacccgata aagacgcgcg gggtggcgag gtgtatctcc tttccggccc
     4381 gacgattatg atgccaggcc acggtcacgg gcatatggaa cacagcaact ggcggatgat
     4441 taacttgccg gttggcccgt tggtggacgg caaaccgatt tatacgctct acatcgcgct
     4501 gtcgatcgat tttcatcttc attacataaa tgatttgatg aataaactta ttatgaccgc
     4561 atcggtaatc agcatcctga tcgtctttat cgtgctgttg gcggtacata aaggtcacgc
     4621 gccgatccgc agcgtcagcc gtcaaatcca gaatatcacc tcgaaagatc tcgacgttcg
     4681 ccttgacccg cagaccgtgc cgattgagct ggaacagctg gtactgtcgt tcaaccatat
     4741 gatcgagcgt attgaggatg tctttactcg ccagtccaat ttctcagcgg atatcgccca
     4801 cgaaattcgc acaccaatta ctaatctcat tacgcaaacg gaaatcgccc tcagccagtc
     4861 gcgcagccag aaggagctgg aagatgtgct ctactctaat ctcgaagagc tgacgcgaat
     4921 ggcgaaaatg gtcagcgata tgctgtttct cgctcaggcc gataacaacc agctaatccc
     4981 cgaaaagaaa atgctcaacc tggcggatga agtcggcaaa gtgttcgatt ttttcgaggc
     5041 gctagcggaa gatcgcggcg tggagttgcg gtttgttggc gacaaacgtc aggttgcggg
     5101 cgatccgttg atgctgcgtc gggcgctaag caacctgctt tctaatgccc ttcgttatac
     5161 gccaaccaga gagacaattg tagtacgctg tcagacggtc gatcaccagg tgcaagtttc
     5221 cgtcgaaaac cccggtacgc ccattgcgcc cgagcactta ccgcgattgt ttgaccgttt
     5281 ctatcgcgtt gccccctccc gccagcgaaa aggtgaaggt agcggtattg ggctggcgat
     5341 agtgaaatcg attgttgtcg cgcataaagg cacggttgcg gtaacgtcag atgcgcgggg
     5401 gacaaggttt gttatcacat tacccgctta attattcagc aaacggcaac ttttataacc
     5461 agtgtaaaaa taacgtgccg caatagtcat acatattaat ggtaaaaagc tgtcacaatt
     5521 cataaaaaac cttaatatac gccaccctaa acataaccag cgttaatgta aggtttttgt
     5581 gtggactggc ttcttgatgt ttttgctacc tggctctacg gcttaaaagt aatcgcgata
     5641 acgttagcgg tcatcatgtt catcagcggg ctggacgatt tttttattga tgtcgtctac
     5701 tgggtacgcc gcattaaacg caagttgagt gtttatcgcc gctacccgcg aatgagttac
     5761 cgcgaactgt ataaaccaga tgaaaaaccg ttagcgatta tggttccggc gtggaatgaa
     5821 acgggcgtca tcggcaatat ggccgagctg gcggcgacca cgctcgacta cgaaaactat
     5881 catatctttg ttggcaccta ccccaacgac cccgatactc aacgtgatgt tgacgaagtg
     5941 tgcgctcgct ttccgaacgt gcataaggta gtctgcgcgc gtcctggtcc caccagcaaa
     6001 gccgactgcc tgaacaacgt gctggacgcc atcacccagt ttgaacgtag cgccaatttc
     6061 gcttttgctg gttttattct gcatgacgcc gaagatgtga tttcaccgat ggaattgcgt
     6121 ctgttcaact atctggtcga gcgtaaagat ctgattcaga tcccagtgta tccgtttgaa
     6181 cgcgaatgga cgcacttcac cagcatgact tacattgatg agttttcaga actgcatggc
     6241 aaagatgttc cggtgcgtga agccctcgcc ggacaagtgc ccagcgcagg cgtcggcacc
     6301 tgtttcagcc gccgcgccgt gaccgcactg ttagctgacg gtaacggtat tgctttcgac
     6361 gtgcagagtc ttactgaaga ttacgacatt ggcttccgcc tgaaagaaaa aggtatgacg
     6421 gaaatttttg tccgttttcc ggtggtggac gaagccaaag aacgcgagca gcgtaaattt
     6481 ttacagcacg cacggacgtc aaacatgatc tgcgtgcgcg aatatttccc cgataccttt
     6541 tcgactgcgg ttcgacaaaa atctcgctgg atcatcggca ttgttttcca gggctttaaa
     6601 acccataaat ggacctccag cctgacgctg aactactttc tctggcgcga ccgcaaaggg
     6661 gcaatcagta actttgtcag cttcctcgcg atgctggtga tgatccagct tttgctgttg
     6721 ctggcgtatg aaagtttgtg gcccgatgcc tggcatttcc tttctatttt tagcggcagc
     6781 gcatggttaa tgaccctgct gtggctaaac tttggcttga tggttaaccg catcgtgcag
     6841 cgagtgattt tcgtcactgg ctactacggc ctcacgcagg gcctgctttc cgtcctgcgt
     6901 cttttctggg gcaacctgat taactttatg gctaactggc gcgtgctgaa acaggttctt
     6961 cagcacggcg atccgcgtcg cgtcgcgtgg gataaaacaa cgcatgactt ccccagcgtc
     7021 actggcgata cccgctcgtt gcgcccgtta ggtcaaattc tgctggaaaa tcaggtcatc
     7081 actgaagaac aactcgatac agcactgcgt aatcgcgtcg aaggtctacg cctgggcggt
     7141 tcaatgctga tgcaggggct gattagcgcc gagcagctgg cacaggcgct ggcagagcaa
     7201 aacggcgtgg cgtgggaatc catcgatgcc tggcagatcc cgtcctcgct gattgccgaa
     7261 atgccagcct ccgtagcgct gcattatgca gtactgccgc tgcgtctgga aaatgatgag
     7321 ttaattgtcg gcagtgaaga tggtattgac ccggtttcgc tggcggccct gacgcgtaaa
     7381 gtcggacgca aagtgcgtta cgtcattgtt ctgcggggac aaattgtcac agggttacgc
     7441 cactggtatg cacgccgacg cggtcacgat ccgcgggcaa tgttgtacaa tgcggttcag
     7501 catcagtggc tcacggaaca gcagaccggt gaaatctggc ggcaatatgt gccgcatcag
     7561 ttcctgttcg ccgaaatact gaccacgctc ggtcatatta atcgttcagc aattaacgtg
     7621 ttgttattgc gccatgaacg cagttctctg ccgctcggca agtttttggt caccgaaggc
     7681 gttatcagcc aggaaacgtt ggatcgcgtc ctgacaattc aacgcgaatt acaagtttcg
     7741 atgcaatcac tattactcaa agcaggttta aacacagaac aggttgcgca actggagtcc
     7801 gaaaatgaag gagaataacc ttaatcgcgt catcggatgg tctggtttac tgctgacgtc
     7861 tttattgagt accagcgcac tcgcagacaa tatcggcacc agcgcagaag agctggggct
     7921 gagcgattat cgccattttg ttatttatcc ccgtctcgat aaggcgctga aggcacagaa
     7981 aaataacgac gaagcaaccg ccatccgcga atttgaatat atacaccaac aggtgccgga
     8041 taatattccg ctgactttat accttgcgga agcctatcgt cattttggtc atgatgaccg
     8101 ggcgcggctg ttgcttgagg atcaactgaa acgtcaccca ggagatgccc gacttgagcg
     8161 cagtctggcg gctattccag ttgaagtgaa aagcgttacg actgttgaag aactgcttgc
     8221 ccagcaaaaa gcgggcgacg ctgcgccgac cctgcgttgt cgcagtgaag tcgggcagaa
     8281 tgccctgcgg ctggcacaat tacctgtcgc cagagcgcaa ctgaacgatg cgacgtttgc
     8341 tgcatcgccg gaaggaaaaa cgctgcgaac cgatctgctg caacgggcaa tctacctgaa
     8401 acaatgatcc caggcagata cgctatacaa tgaagcacgc cagcagaaca cattaagcgc
     8461 ggcagaacgc cgtcagtggt ttgacgtgct tcttgccggg cagctggacg atcggatcct
     8521 ggcactgcaa tcacagggga tcttcaccga tcctcagtca tatattactt acgcgaccgc
     8581 gctggcttat cgtggcgaaa aagcacgcct ccagcattat ctcattgaaa ataagccgct
     8641 gtttaccacg ggcgcacaag agaaaagttg gctctatctg ttatctaaat acagcgccaa
     8701 ccccgttcag gcattggcga attatacggt gcagtttgcc gataaccgcc agtatgttgt
     8761 tggcgcgacg ctaccggtgc tgttaaaaga aggtcagtac gacgcagcgc aaaaactgct
     8821 cgccaccctc cccgccaatg aaatgcttga ggagcgttat gctgtcagcg tggcgacccg
     8881 taacaaggct gaatctctgc gtctggcacg attgctgtat cagcaagaac cgacaaatct
     8941 tccccgcctg gatcaactga cctggcaact gatgcagaac gagcagtcac gcgaagctgc
     9001 cgatttattg ctgcaacgct atcctttcca gggcgatgcg cgtgtcagcc agactttaat
     9061 ggcgcgactg gcgtctctgc tggaaagtca tccttaccta gcaacgccgg cgaaggtggc
     9121 gattttatcg aaacccttac cgctggcgga gcaacgtcag tggcaaagtc agttgccggg
     9181 tattgcagat aattgcccgg caatagttcg cttgctgggc gatatgtcgc cttcctacga
     9241 tgctgccgcc tggaaccgtc tggcaaagtg ttatcgggac acgctacccg gtgtggcgtt
     9301 gtatgcatgg cttcaggccg aacaacgaca accgagcgcc tggcaacatc gtgcggtagc
     9361 ctatcaggcg tatcaggttg aggactacgc caccgcactg gcggcctggc agaaaatcag
     9421 tcttcacgac atgagcaatg atgatctgct cgccgccgcc aacaccgctc aggcagcggg
     9481 aaatggcgca gctcgcgatc gctggcttca acaggcagaa caacgtgagc tgggaaacaa
     9541 tgctcaatac tggtggctgc atgcgcgacg ttatatccca gctcagccag aactcgcact
     9601 gaacgatctc acgcgctcaa tcaatattgc cccttctgcc aactcttacg ttgcgcgggc
     9661 gacaatttat cgccaacgtc ataatgttcc ggccgcggtg agtgatttgc gcgccgcgct
     9721 ggaactggaa ccaaataata gcaacaccca ggcagcgctt ggttacgcct tgtgggatag
     9781 cggtgatatc gcacagtcgc gggaaatgct cgaacaggcg cataaagggc ttccggacga
     9841 tccggcactg atccgacaac tggcctacgt gaaccagcgt ctggatgaca tgcctgcgac
     9901 gcagcactac gcccggctcg tgattgatga cattgataat caggcataac cccactgacc
     9961 ccagagcaaa atcagcaacg cttcaatttc cgccgtttgc atgaggaggt cggtcgccgc
    10021 tggacgttca gtttcgattc ttccatcggc ttgcgttccg gcgcaatgag taccgctaac
    10081 aataatgtcg gcggtgcagc gcctgggaaa agctatcgta gctacggaca actggaagcc
    10141 gagtaccgca tcggacgcaa tatgctgctg gaaggcgacc tgctctcagt ttatagccgc
    10201 gtctttgccg ataccggaga aaacggggtg atgatgccgg tgaaaaatcc gatgtccggc
    10261 accggtctgc gctggaagcc gctgcgcgat cagatctttt tcctcgccgt cgaacagcag
    10321 ttgccgctga acgggcaaaa tggcgcatcc gataccatgc tgcgcgccag cgcctcattc
    10381 tttaatggcg gcaaatacag cgacgaatgg cacccgaacg gttcaggctg gtttgcccaa
    10441 aacctgtacc tcgatgcggc gcaatatatc cgccaggata ttcaggcgtg gacggcagat
    10501 tatcgcgtca gctggcatca gaaggtagct aacggacaga ctattgagcc ttacgctcac
    10561 gttcaggaca acggctatcg tgataaaggc actcagggcg cgcagcttgg cggtgtcggg
    10621 gtccgctgga atatttggac cggcgagacg cactacgacg cctggccgca caaagtcagt
    10681 ctcggcgtcg agtatcaaca tacctttaag gcgattaatc aacgtaacgg agagcgcaac
    10741 aacgcgtttc tcaccattgg agtgcactgg taaatgcgta agttcatttt cgtattgctg
    10801 acactgcttt tggtcagccc tttttccttt gcgatgaaag gtattatctg gcaaccacaa
    10861 aaccgagata gtcaggttac cgatacccag tggcaggggc tgatgagtca gttacgtttt
    10921 caaggcttcg ataccctggt tttgcaatgg acccgttacg gcgatgcatt tacccagcca
    10981 gaacagcgca cgttattgtt taagcgtgcc gcagctgcgc aacagactgg cctgaagctg
    11041 attgtcgggc tgaacgccga tccggaattt tttatgcacc agaaacagtc gtctgcagcg
    11101 ctggaaagct atcttaatcg cctgctggct gccgatctcc agcaagccag attatggagc
    11161 gccgcgcctg gcataacgcc ggatggctgg tacatcagcg cggaaattga cgacctgaac
    11221 tggcgcagcg aagccgcccg tcaacctttg ctaacatggt taaacaacgc gcagcggctg
    11281 attagcgatg tttcagcaaa accggtttat atcagtagtt ttttcgccgg aaacatgtcg
    11341 cccgatggct atcgccaact gctggaacaa gttaaagcaa ccggagttaa tgtctgggta
    11401 caggatggca gcggcgtgga taaacttacc gctgaacagc gtgaacgtta tctacaggcc
    11461 agcgccgatt gccaaagctc cgccccagca agcggcattg tttatgaact gtttgtcgcc
    11521 ggcaaaggca aaacctttac agcgaaaccg aaaccggacg cagaaattgc ctcgctgtta
    11581 gcgaaacgtt cctcttgcgg taaagacatg ctctatttct ctctgcgcta tttgcccgtc
    11641 gcgcacggca ttctcgagta ttaaatcccc tccaggtaag tcgggtacga cctggcttac
    11701 ctctttcgct ctcgtcagaa ttccatcaag attaattgct aaaaagcatg ctaaagaaag
    11761 aagatcgtta tcggcgaacg ggccgatcac tcatgcagga agcatagatc ctttgagcgg
    11821 agtttctgat atctgaggag tgcgaaatgc aattgagcag cagtgaacct tgcgtggtga
    11881 tcctgaccga aaaagaggta gaggtaagcg tcaataacca tgctacgttt acccttccga
    11941 aaaactacct ggccgccttc gcgtgcaaca ataacgtcat tgaactctca acgttaaatc
    12001 acgtattaat cacccacatc aaccgtaaca tcatcaacga ttatctgttg tttttaaata
    12061 agaaattaac ctgtgtaaag ccctggtcgc ggctagcaac cccggttatc gcttgtcata
    12121 gccgtacacc ggaagtgttc cggctagcca ccaaccacag caagcagcaa tccagcaaac
    12181 cctgcgaagc ggagttgacg cgcgcattac tttttaccgt attgtctaac tttcttgagc
    12241 aatcgcggtt tattgcccta ctgatgtata tcttacgcag cagcgtccgc gacagcgtct
    12301 gccgcattat tcaaagcgat attcagcatt actggaatct gcgaattgtc gccagttcgc
    12361 tatgtttaag ccccagcctg ctcaaaaaga aattaaaaaa cgaaaatacc agctatagcc
    12421 agattgtcac agagtgtcgt atgcgttacg ccgtacagat gttattgatg gataacaaaa
    12481 atatcactca ggtggcgcaa ttatgtggct atagcagcac gtcgtacttt atctctgttt
    12541 ttaaggcgtt ttacagcctg acaccgttga attatctcgc caaacagcga caaaaagtga
    12601 tgtggtgaag ggcaaagcgg aaacggataa gacgggcata aatgaagaag aaatggcgcg
    12661 ccctgcagga ttcgaacctg cggcccacga cttagaaggt cgttgctcta tccaactgag
    12721 ctaagggcgc gttgataccg caatgcggtg taatcgcgtg aattatacgg tcaacccttg
    12781 ctgagtcaat ggcttttgat ctggttgctg aacaagtgaa cgaccgcgtc tgattttctg
    12841 atttatttcg ctatagcggc aaacaaacgc acaccgctgc gcgtctgaat caagaaaacc
    12901 cgtattttca tgtatcaaag tacaatttac cgccctaacg gaaaattgtc cgctcctatg
    12961 agactggtaa ctatgaaacc aacgtcggtg atcattatgg atactcatcc tatcatcaga
    13021 atgtctattg aagttctgtt gcaaaaaaac agtgaattgc agattgtcct gaaaacggat
    13081 gattaccgca taaccatcga ttatctccga acccgtcctg ttgatttaat cattatggat
    13141 atagacttgc ccggaacaga cggttttacc ttcct
//
LOCUS       DAJTSF010000100        13142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000100 DAJTSF010000000
VERSION     DAJTSF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.100"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(87..794)
                     /locus_tag="OO335_003187"
     CDS             complement(87..794)
                     /locus_tag="OO335_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6003459.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1192..3327
                     /gene="speC"
                     /locus_tag="OO335_003188"
     CDS             1192..3327
                     /gene="speC"
                     /locus_tag="OO335_003188"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6003460.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3376..4632)
                     /gene="nupG"
                     /locus_tag="OO335_003189"
     CDS             complement(3376..4632)
                     /gene="nupG"
                     /locus_tag="OO335_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6003461.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(4834..5913)
                     /gene="mltC"
                     /locus_tag="OO335_003190"
     CDS             complement(4834..5913)
                     /gene="mltC"
                     /locus_tag="OO335_003190"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6003462.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(5978..6253)
                     /locus_tag="OO335_003191"
     CDS             complement(5978..6253)
                     /locus_tag="OO335_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6003463.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6281..7333)
                     /gene="mutY"
                     /locus_tag="OO335_003192"
     CDS             complement(6281..7333)
                     /gene="mutY"
                     /locus_tag="OO335_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6003464.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7494..8213
                     /gene="trmB"
                     /locus_tag="OO335_003193"
     CDS             7494..8213
                     /gene="trmB"
                     /locus_tag="OO335_003193"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6003465.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHIATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8213..8539
                     /locus_tag="OO335_003194"
     CDS             8213..8539
                     /locus_tag="OO335_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6003466.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            8723..9442
                     /locus_tag="OO335_003195"
     CDS             8723..9442
                     /locus_tag="OO335_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6003467.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9618..10664
                     /gene="ansB"
                     /locus_tag="OO335_003196"
     CDS             9618..10664
                     /gene="ansB"
                     /locus_tag="OO335_003196"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6003468.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            10781..11743
                     /locus_tag="OO335_003197"
     CDS             10781..11743
                     /locus_tag="OO335_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6003469.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(11844..>13142)
                     /locus_tag="OO335_003198"
     CDS             complement(11844..>13142)
                     /locus_tag="OO335_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR6003470.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3452 a         3175 c         3402 g         3113 t
ORIGIN      
        1 gattcgcctc gtctcgtcaa tcaaatttct tcaatttact gcagactgca caatctatca
       61 tcactttgcc gctattgcac gcattgtcag gcaaaccagg cggtccaggc ggcggaaagc
      121 ggcattgcca gaaggagtgc tggaagcatg ttgaccaccg ggaacatttt aatgccacag
      181 atgcgtaatc cggttgccag cagcaataaa ccgcctacgg cgctgaagtc tgccatcatc
      241 gacggtgtga tcagcggtaa tatcagcgcg gcagcccacg ccagcgttaa ctggatgatc
      301 agtaatggga tactaattac cgacaccgca atacccagcg agcaggcgaa gatcatcgcc
      361 gtaaagaaat caagaaatga cttggcgatt aaaatactcg gatcgccggt catcccttcg
      421 ttcatcgccc cgaagatccc ggtgccgctg gcgcaaaaca ggacaataat cgcgacataa
      481 ttctgaataa aagattcatg cgctggcttc ttacgtgagt gacgaaacag attttgtgct
      541 ttggcgactg ctgtattgac acctttctcc agcaaacaaa tttcgccaat tagagcgcca
      601 agtagcgtag ctaaaaccat cgctggaagg ttggcgcatt tcacaaccag taaaatacca
      661 atccccagcg atgccagacc aaaaatagat gtcatggaga cgcggatacg ttccggtaag
      721 cgttggctga gcagtgcgcc gagaacgcca cccagtaaga ctgcactagc gttgataaaa
      781 gggccgatga ccacaagagt tcctgttagc tgtattgttt gattttctta ttatgaactt
      841 tttgccttgt tgatggcttg tctggtcgca aagtcctggc ttgcacgcct tagcgaaagg
      901 tgccatgatt gcgcgaattt tctcctctct gtacggagtt tgcccgatgc acgccatctc
      961 cttacattct ctcgcttatc gccgtttcgc gcgaaacgtt tccctttcta tgttactgct
     1021 catgcggtga agttaacgca cgctcactgc aggacaacag taaaatcaga gcgtttctgc
     1081 ttttactgat gtctggcggt cggagctggt gaccagtttg acccatatct catggggcag
     1141 ggttttccac cttgtccggt attcttactt ccccgaaacg ggtttgcgct tatgaaatca
     1201 atgaatattg ccgccagtag tgaactggta tcccgacttt cttctcatcg ccgcgtggtg
     1261 gcgttgggag atactgattt tacggacgtc gcggcagtcg tcattaccgc tgcggatagt
     1321 cgcagtggca ttcttgcgtt gcttaagcgc accggttttc atctaccggt gtttttgtat
     1381 tccgaacatg ctgttgaatt acctgcgggc gttacggcgg taatcaacgg caacgagcag
     1441 cagtggctgg agctggaatc cgcagcctgt cagtatgaag agaatttgct gccaccgttt
     1501 tatgacacgc tgacgcagta cgttgagatg ggcaacagca cctttgcttg ccctggacat
     1561 caacatggtg cgttctttaa aaagcatcct gccggacgcc atttttacga tttctttggt
     1621 gagaacgtct ttcgcgccga tatgtgtaac gctgacgtaa aattgggcga tctgcttatt
     1681 catgaaggat cggcgaaaga tgcgcagaaa ttcgcagcca aagtctttca tgccgataaa
     1741 acctattttg tgctgaacgg cacatcggca gcgaataaag tggtgacgaa tgcgctgtta
     1801 acgcgtggcg atctggtgct cttcgaccgt aacaaccata agtcgaatca tcacggcgcg
     1861 ctgattcagg cggggacgac gccggtctat ctggaagctt cacgtaaccc gtttggtttc
     1921 attggcggta ttgatgcgca ctgttttaat gaagagtatc tgcgccagca aattcgcgac
     1981 gttgcgccag aaaaagccga cctgccgcgc ccgtttcgcc tgacgattat tcagctggga
     2041 acctatgacg gcactgtcta taacgcccgt caggtgatcg ataccattgg gcatctgtgt
     2101 gattacattc tgtttgattc cgcgtgggtc ggttacgaac agtttatctc gatgatggcg
     2161 gatagctcgc cgctgctgtt agaacttaac gaaaacgatc cggggatttt tgtgacccag
     2221 ttggtgcaca aacagcaggc tggattctca cagacgtcgc agatccataa aaaagataac
     2281 catatccgcg gacaggcgcg tttttgcccg cataagcggt tgaataacgc ctttatgctc
     2341 catgcttcta ccagcccttt ctatccgctg tttgctgcac tggatgttaa cgccaaaatt
     2401 catgaagggg agagtgggcg tcggctgtgg gctgagtgcg ttgagttggg tattgagtcg
     2461 cgcaaggcta ttcttgcgcg ctgtaagctg ttccgcccgt ttatcccgcc cgttgttgat
     2521 ggcaaattgt ggcaggatta tccgacatca gtgttagcca gcgaccgccg ttttttcagt
     2581 tttgagccgg gggcgaagtg gcacggcttt gaaggatatg ccgcggatca gtattttgtt
     2641 gatccgtgca agctgttact cactacacca ggtatcaatg ccgaaaccgg cgaatatagc
     2701 gactttggcg ttccggcgac gattctggcg cactatctgc gtgagaacgg cattgtgccg
     2761 gagaagtgcg atctcaactc cattctgttc ttattaactc cggcggaaag ccacgagaag
     2821 ctggcacaac tggtggcgat gctggcgcaa tttgaacagc atattgagga tgactcgtcg
     2881 ctggctgagg tgttgccgag cgtttataac aaatatccgg tgcgctatcg cgactacacc
     2941 ctgcgccagt tgtgtcagga gatgcacgat ctgtatgtca gtttcgacgt caaagaccta
     3001 caaaaagcga tgttccgcca gcagagtttc ccgtcagtgg tgatgaaccc ccaggatgcg
     3061 catagcgctt atattcgcgg tgaagtggag ttggtgcgga ttcgtgatgc cgaagggcga
     3121 attgcggcag aaggggcgtt gccttatcca cctggcgtgc tttgcgtggt acccggggaa
     3181 gtctggggtg gggcggttca acgttatttc cttgcgctgg aagaaggcgt gaatttgttg
     3241 ccgggatttt cgccggagtt gcaaggtgtc tatagcgaaa ccgatgcgga tggcatgaaa
     3301 cgattgtacg gttatgtgtt gaagtaagaa taaaaaaacg ggtcaccttc tggcgacccg
     3361 tttttctttg cgtaattagt ggctaaccgt ctgtgtgcct gtcgggacac gaacgtgttt
     3421 atatttgaac atcgccatga acgcgaaggc cagaaccacg gaataaccag cgaaaatcaa
     3481 ccatacggtc tgccagtcgg taatgccgtt ttgggtgtac atctcaacaa ctttaccgct
     3541 cacgatgccg ccgaggatac agccgaagcc gttagtcatc atcaggaaca tcccttgtgc
     3601 actggcgcga attgccgggc taacttcttt ttcgacaaac accgaaccag agatgttgaa
     3661 gaagtcgaat gcgcaaccgt aaacgatcat cgacagtacc agcagtacag taccgaacgg
     3721 agtcgggtcg ccgtaagcaa acagcgcaaa acgcaggatc cacgccacaa tactgatcat
     3781 cattacgttc ttaataccgt agcggcttaa gaagaacggg atggtcagaa tgaacagggt
     3841 ttcagagatc tgcgaaatcg acatgatgat tgacgcatgc tgcacgatga agctgctggc
     3901 aaacatcgga tctttgtcga agctgtgcag gaaggtatta ccgaacatgt tggtaatctg
     3961 cagttccgcg cccagcagca ttgagaagat gaagaagatt gccatacgct tgtttttaaa
     4021 cagcgcgaat gcatcgaggc ccagcagggt tgtccagctc tgattcgctt gctgtttagc
     4081 aaccggaata tgcggcagag tcagggtaaa cagaaccaga atggcggaaa gtgctgcgcc
     4141 aatatacagc tgcatgtggc ttaattcgaa gccagacagg ctcaccaccc acattgccat
     4201 gataaagccg atggtgcccc agatacggat tggcgggaag tcagtaacga tatccatccc
     4261 ggcattttgc aggcgatagt aagagatggt gttgattaac ccaagcgttg gcatataagc
     4321 aaacgagtta atcaatatca caaggaacat cgcttccggc gtcgtgacct gtgccgccat
     4381 gaacagcgtg atagcgccaa tggtgtggca aatggcatat acccatttcg cacttaacca
     4441 tttgtcggcc acaatcccca gcagcgcagg cataaatacc gcggcgatac ccagtgagct
     4501 ataaactgcg ccaatagaag caccgtcaaa cttcagggta acaaacatat aggagccgag
     4561 ggtcgtcagc caacttcccc acagacagaa ctgcagaaaa gagaggattt tcagctgcag
     4621 cttaagattc atgttaattt cctcacatcg tgatgcggat gagtgtttga aaaagcacat
     4681 ttgaccgtgc gagatgatgg gattctatca acttcgcgga ctggtttttt gttacctgtg
     4741 gcaaataatt gcaaacattc ccaatttgca aaacgaaatt gtgatgtgga taacattttt
     4801 gcccctgagc atcgtcaggg gcggttaatg gaattatcgg cggcggtagg atttttgcgc
     4861 ggtattcact ttataaagat aacggcgaga ttccgcagag ggatggcggg tcgtcagcgt
     4921 ctgataaaca tcgcccggcg tcatggtgtt aataatattg gcagcctgaa tcttatcatt
     4981 cgaaaagact cgcagcacgc tgcctgcgcc gccgttatag gcggtgatga cggcataacg
     5041 ccgcgatgtt gggttatcaa ttccgccgag ataaacattg ttcagcatcg ccagatacgc
     5101 ggtgccggta tcaatattgc tggcaggatc aaacaagaaa ctgcggctcg gcgtgccgga
     5161 tttcccctgc gagcggaaca catctttccc ggcagtattt tgtaccacct gcattaatcc
     5221 cagcgcatcg gaacggctga ccgcatacgg gttaaaggaa gattcagtct gcataattgc
     5281 cagaatcagc gactcatcaa cgccatattt ccgtgacgcc tggcggacca tgccgagata
     5341 tttgtgcgca cgtttatcaa ggtggttcgg caccatgtta atggtgacgc tgtagatgat
     5401 acgtagcccg ttgctgcggc ttttcagacg gtttttcagc agataatccg cgaagttgct
     5461 tgcgcgacct tcccagcgaa tcggctgccc ggtgttgtcc accacctgac cgtaaaggaa
     5521 aggttctttc gaaattgtaa tatcatcaac gtcggaatag agatcgaccg aactcggatc
     5581 gtcacccatc agcaacgttt tgataattgc ccggcgcaaa tgcgcggcag gttctgtccc
     5641 ggcgatggtt tcgatagtaa tcgtaccgtc atcgaagttg atgtggctgc gggtctgata
     5701 ttgatcggtg tatttcacgt agtccttagg accggcgatc accacctctt tgaagcccca
     5761 gatgttctca atattgtggg caaattgccc catcagaata tcaaaaccgt tggtatcttt
     5821 gacccaggct tcgttatagg tatcgccttt tttggtcgtc gaacaggaga tgagcaacgg
     5881 cgcaatcaaa gccagcgcga gatatttttt catcattccg ggtgcgtgtt gtgtttgttt
     5941 ttatgcaagc tgccggaggc gtgctccggc actgttttta ttttttatct tccggcgtat
     6001 agccctcgat atgcacctct ttaccctcga acaggaagtt gaccatctcc tgctccagca
     6061 gcttgcggtg ctcggcattc atcatgttga gtttcttttc attaatcagc atggtttgtt
     6121 tgtgctgcca ctgcgcccag gcttctttgg agatctcgtt atagatgcgt tttcccagct
     6181 cgccggggta cagctgaaaa tcctgacctt ctgcttcacg ttgcaggaaa gtacaaaaaa
     6241 tcgttctgct cataaatcat cctctttatc gactcacgcg ctaaaccggc gcgccagtgc
     6301 gtaactgctg taacaaacgc tccacgggag ccgccaggcc aactgacggc ggttgcgcta
     6361 agttatacca gagcgcattg ccttcatcca tgcagccggt gaatgacgac acgggaagcc
     6421 acataggcac aatatctaag tggaaatggc tgaaggtatg gcgaaacgcg gtcagttgcg
     6481 tcaggttatc ggcagcaatc tgccgttgtg ccagccaatg ccgcaaactt tcttcgtcgg
     6541 caaactgcgg gaaacagtat aaaccgcccc acaatccgct cggcggacgc tgcgccagca
     6601 atacttcatc ttcgtgctgt aacaggagaa agtagccggt gcgctccggc agcgtctgtt
     6661 tcggtttttt gcccggataa agcgaccagc tattgttggt ggcggcaata catccgtttt
     6721 gtagcggaca gagcgaacat ttcggcttcg aacgcgtgca aatcatcgcg cccaaatcca
     6781 tcatcgcctg attaaaccgt tccacgccga ccgcgggcgt cacctgctcg ctcaaactcc
     6841 atagtttatt ctcgacctct tttttcccag gccagccgct tacagcatag cagcgcgcca
     6901 gcacgcgttt gacgttaccg tcgagaatcg gaaagtgctt acccagagaa agcgagagaa
     6961 tcgcgcctgc ggtggaacgc ccgacgcccg gtaacgccgc gacttcttca aaggtttccg
     7021 ggaatttacc accgtgtaag gtcgccactt gttgtgccgc tttatgcagg ttgcgcgcgc
     7081 gggcgtaata gccaagcccg gtccacaagt ggagaacttc gtcgagcggc gcattggcga
     7141 gatcggtcac cgtcgggaag tgcgccataa agcgttcaaa ataggggata acggtcgcaa
     7201 cctgagtttg ttgcaacatc acttctgaga gccatacttt gtagggcgtc ttgtcaattt
     7261 gccagggcag cgttttccgc ccgtatttat cgtaccagtc cagaacctgg gctgaaaatt
     7321 gcgacgcttg catggtcacc gacttcactg ttgttggggg caagattgca gcacagcgac
     7381 ggcagggtgt aaaccggaac tttccgcagc cacggcctta tcatgcttgc atccggcaac
     7441 taactttgga taatgcccgt tttcagaaca ctttcacaag cgactaaatc tttatgaaaa
     7501 acgacgtcat ttcaccggaa tttgatgaaa acggccgccc actgcgccgt atccgtagtt
     7561 ttgtgcgccg ccaggggcga ctgaccaaag gccaggaaca tgcgctggaa aactactggc
     7621 cggtgatggg cgttgagttc agtgaagata tgctggattt ccccgcgctt tttggccgtg
     7681 aagcgccggt gacgcttgag attggttttg gcatgggggc gtcgctggtg gcaatggcta
     7741 aagatcgccc cgagcaggac ttcctcggca ttgaagtgca ttcaccgggc gttggtgcgt
     7801 gcctggcttc tgcgcatgaa gaaggtttaa gcaacctgcg cgtgatgtgt cacgatgcgg
     7861 ttgaagtgct gcataaaatg attcctgaca attcattgcg catggtgcag ctctttttcc
     7921 ctgacccgtg gcacaaagcg cgccataata aacgccgtat cgttcaggtg ccgtttgccg
     7981 aactggtaaa aagcaaactg cagctggggg gcgtattcca tatagcgacc gactgggaac
     8041 cttatgcgga acatatgctt gaagtgatgt cttctattga cggttataaa aacctgtcag
     8101 agagcaatga ttacgtaccg cgtccggcat cacgtccggt gacgaaattt gaacaacgtg
     8161 gtcatcgtct tggtcacgga gtatgggact taatgttcga gagggtgaaa taatggcaaa
     8221 gaaccgtagc cgtcgtctgc gtaaaaaaat gcacatcgac gaattccagg aattaggatt
     8281 ttcggtggca tggcgattcc cggaaggtac atcggaagaa cagattgata aaatcgttga
     8341 tgactttatt aacgaggtta tcgaaccgaa caaactggcc tttgacggca gcggttatct
     8401 ggcctgggaa ggtctgatct gcatgcagga aatcggcaaa tgcaccgaag aacatcaggc
     8461 gattgtgcgt aagtggctgg aagagtgcaa actggatgag gtacgcacca gcgaactttt
     8521 cgacgtttgg tgggactaag aaagcatacg ggcgatgaca aatgcaaaac tgcctgatgc
     8581 gctacgctta tcaggcctac agagatgcac gatcgagtag gcgggataag gcgtttacgc
     8641 cgcatccggc atggaaaacg cgtactttgt tatcaatctg gggccagcat atgctggcct
     8701 gatttgttct tgagggaaga ctatgatgcg caaaatgctg ctggcggcag cactttcagt
     8761 gacggcaatg accgctcacg ccgactacca gtgcagcgtc acgccgcgtg acgatgtgat
     8821 tgtcagcccg caaaccgtgc aggtgaaggg cgaaaacggc aatctggtga tcacgccaga
     8881 cggcaacgtg atgtataacg gtaagcaata ttccctgaat gccgcccagc gcgagcaggc
     8941 gaaggattat caggctgaac tacgtagcac gctgccgtgg attgatgaag gcgcgaaaag
     9001 ccgcgtcgag aaagcccgta ttgcgctgga taaaattatc gttcaggaga tgggcgacag
     9061 cagcaaaatg cgcagccgtc tgaccaaact tgatgcgcag cttaaagagc agatgaatcg
     9121 cattatcgaa acgcgcagcg atggcctgac gttccactat aaagccattg atcaggttcg
     9181 cgccgaaggc cagcaactag tgaatcaggc aatgggcgga attttacagg acagcattaa
     9241 tgaaatgggc gcgaaagcgg tgctgaaaag cggcggtaac ccattacaga atgtgctggg
     9301 aagcttgggc ggtctgcaat cctcaatcca aaccgagtgg aaaaagcagg aaaaagattt
     9361 ccagcagttt ggcaaagatg tttgtagccg cgttgtgact ctggaagata gccgcaaagc
     9421 cctggtcggg aatttaaaat aatcctctat tttaagacgg cataatactt ttttatgccg
     9481 tttaattctt cgttttgtta cctgcctcta actttgtaga tctccaaaat atattcacgt
     9541 tgtaaattgt ttaacgtcaa atttcccatg caaagctaag ggataatgcg tagcgtccac
     9601 gtaactggag gaatgaaatg gagtttttca aaaagacggc acttgccgca ctggttatgg
     9661 gttttagtgg tgcagcattg gcattaccca atatcaccat tttagcaacc ggcgggacca
     9721 ttgccggtgg tggtgactcc gcaaccaaat ctaactacac agcgggtaaa gttggcgtag
     9781 aaaatctggt taatgcggtg ccgcaactaa aagacattgc gaacgttaaa ggcgagcagg
     9841 tagtgaatat cggctcccag gacatgaacg ataatgtctg gctgacactg gcgaaaaaaa
     9901 ttaacgccga ctgcgataaa accgacggct tcgtcattac ccacggtacc gacacgatgg
     9961 aagaaactgc ttacttcctc gacctgacgg tgaaatgcga caaaccggtg gtgatggtcg
    10021 gcgcaatgcg cccgtccacg tccatgagcg cagacggtcc attcaacctg tataacgcgg
    10081 tagtgaccgc agctgataaa gcctccgcta atcgtggcgt gctggtggtg atgaacgaca
    10141 ccgtactgga cggtcgcgat gtcaccaaaa ccaacaccac cgacgtagcg accttcaagt
    10201 ctgttaacta cggtcctctg ggatacattc acaacggtaa gattgattac caacgtaccc
    10261 cggcacgtaa gcacaccagc gatacgccat tcgatgtctc taagctgaat gagctgccga
    10321 aagtcggcat cgtttataac tacgctaacg catccgatct tccggctaaa gcactggtag
    10381 atgcgggcta tgatggcatc gttagcgctg gtgtgggtaa tggtaacctg tataaatccg
    10441 tgttcgacac cctggcaacc gccgcgaaaa acggcactgc agtagtgcgt tcttcccgcg
    10501 taccgacggg tgctaccact caggatgctg aagtggatga tgcgaaatac ggcttcgtcg
    10561 cctctggcac gctgaacccg caaaaagcgc gcgtcctgct gcagctggct ctgacgcaaa
    10621 ccaaagatcc gcagcagatc cagcagattt tcaatcagta ctaatcacct cgtcccggca
    10681 tcgtgccggg gctttttcac ttcagactta cgtccattgc caattttcat taccctaatg
    10741 ataatcaccg gaataaatta ttccgcgcga gggttttcgg gtgaaaaagc aatggattgt
    10801 tggcacggcg ctgcttatgt tgatgactgg taatgcctgg gcagatggcg aaccgccaac
    10861 tgaaaatatc ttaaaagatc aattcaaaaa gcagtatcac ggcattctca agcttgatgc
    10921 catcagctta aaaaatcttg atgctaaggg taatcaggcc acctggtcag cggaaggcga
    10981 tgtctcttcc agtgacgatc tctatacctg ggtcggtcag ttggcagatt acgaactgct
    11041 cgaacagacc tggacgaaag ataaaccggt aaaattctcg gcgatgttaa ccagtaaagg
    11101 aacgcccgcg tctggctggt cggtgaactt ttactctttt caggcggcag ccagcgatcg
    11161 tgggcgggtg gtcgacgata tcaaaacgaa taataaatat ctgatcgtga atagcgaaga
    11221 tttcaattat cgctttagtc agcttgaatc tgcgttgaat acccagaaga actcgattcc
    11281 tgcgctggaa aaggaagtga aagcgcttga taagcaaatg gtggcggcgc agaaagcagc
    11341 ggatgcatat tggggaaaag atgcaaacgg taagcaaatg acccgcgaag aagcctttaa
    11401 aaaaattcac caacagcgtg atgagtttaa taaacagaac gatagcgagg cgttcgcggt
    11461 taaatatgac aaagaggttt atcaaccggc gattgcggca tgtcataaac agagtgaaga
    11521 gtgttatgaa gtgccgattc agcagaaacg agatttcgat atcaacgaac aacggcgaca
    11581 gacttttctg caatcacaaa aactcagccg taaattgcag gatgactggg taacgctcga
    11641 aaaagggcaa tatccgttaa cgatgaaagt ttcagaaata aacagtaaaa aagtcgcgat
    11701 tttgatgaaa attgacgata tcaaccaagc taatgaacgc tagaaaaaag ataccgaaca
    11761 actgcggcgc aatggggtga ttaagtaact catgttaagt gcctttgccg gataatataa
    11821 aaagttatcc ggcaaattat tttttacatt agccctagta gctgtggaat gaacaaggat
    11881 atttgcggaa tgtaagtgat catcatcaac gctactagca atgcagcata aaatagcagc
    11941 aatggtttga ttaaatgttg aatcttcaca cccgagatag aacaaccaac aaatagcgca
    12001 ctacccaccg gcggtgttaa cagcccaata caaaggttgg ccaccatcat aataccgaaa
    12061 tgtactgggt ccattcccag ctcttgtgca atcggcaaga agatcggtgt aaagattagt
    12121 acagccggtg tcatgtccat aaagatgccg acaattaata agacgatatt aatcagcagc
    12181 aatatgatca gtgggttatc agaaatcccc atcaatgcgt cgctgatcat ataaggaata
    12241 tcagcattag tcattgccca ggacatgccc acggaaaaac cgatcaacag taatacaatc
    12301 gaagtggtaa caaccgactc cagaatcagt ttgggtaaat ggcgccattt tacttcccgg
    12361 taaatcagca ccgagagaat aaatgtgtag acaacggcaa ttgctgacgc ttcagttgcg
    12421 gtaaaaatgc cgcccagaat gccgcccatc acaatgaaca ccagaagcaa acttggtaag
    12481 gcatccagaa acgctttaca ggcctgagca aaggttgctc tttctgaaag cgggtagcca
    12541 cggcgtttag caataatgcc gcagactatc attaccgcaa gacccattaa gatccctggc
    12601 aaatatcccg ccataaacag cgatgctaca gaaacgccgc cagctgtcag ggaaaacaca
    12661 attaacacgt ttgagggcgg tatcagtaat ccagtaatac aagatgagac gtttacagcg
    12721 gtagaaaatg ccggatcata accttctttg gtctgaatgg gattcagtgt gccaccaacg
    12781 gcagcagctg ccgcaacggc tgaaccagaa atggaaccaa acatcatatt cgccaggacg
    12841 ttcacatgac caagcgaacc aggaacgcgg ccaaccatta cctgggcgag gttaatcaaa
    12901 cgtatcgcaa taccgccgct gttcatcagt gttccggcaa agatgaaaaa ggggattgcc
    12961 aacagggcaa agttatccag tccattagcc agtcgttgcg aaacagtgat tgccgcgata
    13021 tcccagggaa acatcaaaag cattgaagct acagtggcaa taccgattga gaacgaaatg
    13081 ggcacaccaa taaagaccag cacaaaaaaa cagccaaaca gtgtaatcgc gatataacta
    13141 tc
//
LOCUS       DAJTSF010000101        13096 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000101 DAJTSF010000000
VERSION     DAJTSF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.101"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..890)
                     /locus_tag="OO335_003199"
     CDS             complement(<1..890)
                     /locus_tag="OO335_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6003471.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="OO335_003200"
     CDS             1131..1931
                     /locus_tag="OO335_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6003472.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="OO335_003201"
     CDS             2009..2779
                     /locus_tag="OO335_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6003473.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="OO335_003202"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="OO335_003202"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6003474.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="OO335_003203"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="OO335_003203"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6003475.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="OO335_003204"
     CDS             5034..5756
                     /locus_tag="OO335_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6003476.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="OO335_003205"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="OO335_003205"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6003477.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="OO335_003206"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="OO335_003206"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6003478.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="OO335_003207"
     tRNA            7149..7225
                     /locus_tag="OO335_003207"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="OO335_003208"
     CDS             complement(7356..8402)
                     /locus_tag="OO335_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR6003479.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="OO335_003209"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="OO335_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="OO335_003210"
     CDS             complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="OO335_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR6003480.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="OO335_003211"
     CDS             complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="OO335_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR6003481.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11362..12192)
                     /locus_tag="OO335_003212"
                     /pseudo
     CDS             complement(11362..12192)
                     /locus_tag="OO335_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="OO335_003213"
                     /pseudo
     CDS             complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="OO335_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3007 a         3393 c         3482 g         3214 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tcccctctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg
     9421 ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc
     9481 cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt
     9541 ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg
     9601 agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct
     9661 cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga
     9721 gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg
     9781 agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc
     9841 tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga
     9901 acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca
     9961 gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg
    10021 tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac
    10081 cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca
    10141 taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca
    10201 ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc
    10261 cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa
    10321 aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt
    10381 gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc
    10441 acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg
    10501 cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg
    10561 tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga
    10621 ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc
    10681 gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg
    10741 ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg
    10801 tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc
    10861 tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc
    10921 ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt
    10981 tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca
    11041 gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt
    11101 gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg
    11161 cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg
    11221 acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc
    11281 gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc
    11341 ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt
    11401 ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc
    11461 ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga
    11521 gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc
    11581 attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg
    11641 cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag
    11701 ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt
    11761 ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc
    11821 ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg
    11881 cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc
    11941 acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc
    12001 gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa
    12061 caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg
    12121 aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt
    12181 attgtttgtc atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa
    12241 tgccaatgat gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt
    12301 ccggggcgta ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc
    12361 agatagtttc atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc
    12421 caccagtgac catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca
    12481 gatcctgcga ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt
    12541 gttcgggaga taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt
    12601 tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt
    12661 gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg
    12721 acgtgatggc agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc
    12781 catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca
    12841 tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat
    12901 tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat
    12961 tcaccttaaa aacaggaatc cagggagagg taacggtgaa gataatttgt gttccttcag
    13021 tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa
    13081 tatttggttt cattgt
//
LOCUS       DAJTSF010000102        12906 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000102 DAJTSF010000000
VERSION     DAJTSF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.102"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO335_003214"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO335_003214"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR6003482.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="OO335_003215"
     CDS             complement(1806..3224)
                     /locus_tag="OO335_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6003483.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="OO335_003216"
     CDS             complement(3378..3722)
                     /locus_tag="OO335_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR6003484.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO335_003217"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO335_003217"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6003485.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="OO335_003218"
     CDS             complement(4712..5647)
                     /locus_tag="OO335_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR6003486.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="OO335_003219"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="OO335_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR6003487.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="OO335_003220"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="OO335_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6003488.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO335_003221"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO335_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6003489.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="OO335_003222"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="OO335_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6003490.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="OO335_003223"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="OO335_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR6003491.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="OO335_003224"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="OO335_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6003492.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OO335_003225"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OO335_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR6003493.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11365..12483
                     /gene="ydeE"
                     /locus_tag="OO335_003226"
     CDS             11365..12483
                     /gene="ydeE"
                     /locus_tag="OO335_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR6003494.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="OO335_003227"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="OO335_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR6003495.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3400 a         3299 c         2949 g         3258 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaat
//
LOCUS       DAJTSF010000103        12874 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000103 DAJTSF010000000
VERSION     DAJTSF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.103"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1177)
                     /locus_tag="OO335_003228"
     CDS             complement(<1..1177)
                     /locus_tag="OO335_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6003496.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1376..1460)
                     /locus_tag="OO335_003229"
     tRNA            complement(1376..1460)
                     /locus_tag="OO335_003229"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1424..1426),aa:Leu,seq:caa)
     gene            1656..2675
                     /gene="ahr"
                     /locus_tag="OO335_003230"
     CDS             1656..2675
                     /gene="ahr"
                     /locus_tag="OO335_003230"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6003497.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2804..4306
                     /locus_tag="OO335_003231"
     CDS             2804..4306
                     /locus_tag="OO335_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6003498.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="OO335_003232"
     CDS             complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="OO335_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6003499.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="OO335_003233"
     CDS             complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="OO335_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6003500.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6874..8385
                     /gene="pepA"
                     /locus_tag="OO335_003234"
     CDS             6874..8385
                     /gene="pepA"
                     /locus_tag="OO335_003234"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6003501.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8519..8962
                     /gene="holC"
                     /locus_tag="OO335_003235"
     CDS             8519..8962
                     /gene="holC"
                     /locus_tag="OO335_003235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6003502.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8962..11817
                     /gene="valS"
                     /locus_tag="OO335_003236"
     CDS             8962..11817
                     /gene="valS"
                     /locus_tag="OO335_003236"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6003503.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11871..>12874)
                     /locus_tag="OO335_003237"
     CDS             complement(11871..>12874)
                     /locus_tag="OO335_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCR6003504.1"
                     /translation="SYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCVLTLLLLV
                     LLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTVFFISTKM
                     LHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTAY
                     CIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQRKMIIAQ
                     LIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGIT
                     GGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVMPSLPFL"
BASE COUNT         3280 a         3340 c         3377 g         2877 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tcctatggtg
     1381 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1441 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1501 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1561 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1621 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1681 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1741 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1801 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1861 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1921 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1981 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     2041 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2101 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2161 ccagccgcgt tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     2221 acgcaatggg atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac
     2281 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt
     2341 cggggcagtt tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt
     2401 ttgaggcgct gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     2461 ctgttccggc ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca
     2521 cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa
     2581 ccgaactgtt cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag
     2641 cgcgctaccg agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg
     2701 gcacttaaat ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca
     2761 cagccgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg aacccctgtt
     2821 aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta accgtcacgg
     2881 gctgattact ggcgcaacgg ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc
     2941 attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg aaaggcgatc tgaccggtat
     3001 cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg cttaaaaata tcggcgtcaa
     3061 tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg agaaaggcca
     3121 cacggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcgcgac tgttgaatct
     3181 caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg atcagggatt
     3241 gttgctgctc gactttaaag atttgcgggc gattacccag tacatcggcg ataacgccaa
     3301 atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg
     3361 attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga tgctggatat
     3421 caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc tcagcgccga
     3481 gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc tttcagagtt
     3541 gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt ttttcttcga
     3601 cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga ttgagcaggt
     3661 gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga
     3721 tattccggat aatgtgttcg gccagctcgg taatcgtgtt cagcacgctt tgcgggcttt
     3781 tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa accatgcggg ccaatccggc
     3841 atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa tctcgtttct
     3901 cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg
     3961 aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactccc cggtgtatgg
     4021 caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa aaggctttca
     4081 ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg aaagaggtgg cggtggatga
     4141 cggtattctt ggtggattga aggatatttt gtttggcact accggaccac gcggcgggaa
     4201 gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga cgaatcagat
     4261 tgtacgcggg atgttgggga gtttgctggg ggggagaaaa agataagtca gacgtgatct
     4321 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4381 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4441 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4501 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4561 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4621 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4681 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4741 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4801 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4861 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4921 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4981 ctgatgccac ctaacacctc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
     5041 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5101 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5161 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5221 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5281 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5341 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5401 acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
     5461 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5521 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5581 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5641 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5701 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5761 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5821 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5881 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5941 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     6001 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6061 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6121 tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6181 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6241 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6301 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6361 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6421 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6481 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6541 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6601 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6661 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6721 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6781 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6841 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6901 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6961 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     7021 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7081 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7141 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7201 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7261 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7321 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7381 ggtgagcgcg cgatccagca cggtcaggcg attgccgccg ggataaaagc agcgaaagat
     7441 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7501 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7561 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7621 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7681 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7741 atgaagtacg atatgtgcgg cgcggcggca gtttacggcg tgatgcgtat ggtcgcggag
     7801 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7861 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7921 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7981 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     8041 catcatatta ctggtctgat gacgaaccat aatccgctgg cccatgaact gattgccgcg
     8101 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8161 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8221 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8281 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8341 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8401 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8461 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8521 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8581 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8641 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8701 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcagtg cgccggtgga
     8761 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8821 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8881 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8941 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     9001 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9061 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9121 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9181 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9241 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9301 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9361 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9421 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9481 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9541 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9601 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9661 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9721 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9781 ggctgcgtga agatcacacc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9841 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9901 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9961 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
    10021 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10081 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10141 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10201 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10261 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10321 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10381 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10441 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10501 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10561 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10621 aagatgtcca aatccaaggg taacgttatt gacccgctgg atatggttga cggtatttcg
    10681 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10741 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10801 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10861 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10921 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10981 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    11041 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11101 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11161 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11221 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11281 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11341 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11401 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11461 gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatt
    11521 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11581 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11641 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11701 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt
    11761 tatgcagaag cgaaagccaa gctgattgaa cagcaggctg ttatcgccgc actgtaatca
    11821 acacagtgat gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat
    11881 ggtaaagaag gcataactcc ccgagaaata ctggctaaga agcctttatc tggttgttct
    11941 tctttattga ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc
    12001 cagtcaatca tccatatttt cagcagtggg taagccagac cacccgtaat cccggatatc
    12061 accaccaacg cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga
    12121 atacgcccat cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa
    12181 taactggtgc taacagcaat cccaaaataa taaattaact gcgcgattat cattttacgt
    12241 tgcatttcca taaattgctg taaattctca atatcatcat ttgcataccc agaactatca
    12301 tactcattta atatttgatc gaagataata taaccagcaa cagcgagaaa aggtaataaa
    12361 gctaaaaatg cgaggatggc atattttata caatacgcag tatctaattt caccttgaaa
    12421 cgatgtatac cgaaactggt attgctccag agaaaactca ttaccaggct atataaagta
    12481 ccattaaaaa taccaatgga aacaatacca actattgcca tcagaaccac agatataata
    12541 acactacttg aactattggc atgtagcatc tttgtcgaga taaagaaaac agtccccatc
    12601 ccaatggcca ttaaaatcgg caagaaaaag gttacccacc agaacccttt caaagagcaa
    12661 ttaaaactaa atcttacgcc gttgagactg gtcattaagg cctgataacg cagtcctttt
    12721 gctgccataa atataagcaa aaccaacagt aacaaagtca aaacacagcc aacaagcggc
    12781 atatctgctg acactgtcat taagattgcg aaatagaaaa aaacaaaaac aagacaacta
    12841 acaaaaacat tcccaccggt aattccataa gaaa
//
LOCUS       DAJTSF010000104        12662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000104 DAJTSF010000000
VERSION     DAJTSF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.104"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="OO335_003238"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="OO335_003238"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6003505.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="OO335_003239"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="OO335_003239"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR6003506.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="OO335_003240"
     CDS             3115..3399
                     /locus_tag="OO335_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6003507.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OO335_003241"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OO335_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6003508.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OO335_003242"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OO335_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6003509.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OO335_003243"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OO335_003243"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6003510.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="OO335_003244"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="OO335_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6003511.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9585..10685
                     /gene="ompC"
                     /locus_tag="OO335_003245"
     CDS             9585..10685
                     /gene="ompC"
                     /locus_tag="OO335_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6003512.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10849..11334
                     /locus_tag="OO335_003246"
     CDS             10849..11334
                     /locus_tag="OO335_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6003513.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11444..12433
                     /locus_tag="OO335_003247"
     CDS             11444..12433
                     /locus_tag="OO335_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6003514.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3341 a         3128 c         2941 g         3252 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9421 atctcatttc gacttcgatc acatatcgat aaataatatt attctatttt tctcattgtt
     9481 tttaatcacc attctatttt aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt
     9541 cgtttagaaa atacgtttaa cgataattaa aaggattctg ttaaatgaaa ttaaaaatag
     9601 ttgcggtggt tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata
     9661 acaaggatgg taataaactc gacctttatg gcaaggttac cgctctacgt tattttactg
     9721 atgataagcg tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc
     9781 aaatcaatga tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg
     9841 atgaagccaa cggctcgcgc ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta
     9901 gtgaatttgg ctctctggac tatggtcgta actacggtgt cggctacgac attggttcat
     9961 ggaccgatat gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga
    10021 cataccgtac caccggtcta gcaacctatc gcaactacga tttctttggc ttaattgaag
    10081 ggctgaactt tgccgcgcaa tatcaaggca aaaataaacg tactgacaac agtcatcttt
    10141 atggtgctga cgacacgcgt gccaacggtg acggtttcgg tatctcctca acttatgttt
    10201 atgatggctt tggtatcggt gcggtatata ccaaatccga tcggacaaat gcgcaggaaa
    10261 gagccgcagc taatcctctc aatgcctccg gtaagaatgc agaactatgg gccacaggta
    10321 taaagtatga tgccaacaac atctactttg cagctaatta cgatgaaaca ttaaacatga
    10381 ccacctatgg ggatggttat atctctaaca aagcacaaag ttttgaagtg gtggcgcaat
    10441 atcaattcga cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc
    10501 tgggccgcta cgccgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct
    10561 tcaacaaaaa tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact
    10621 ttacccgtgc cgtagatatt cgcaccgata acatcgtcgc tacgggcatt acctatcagt
    10681 tctaacgtcc tctcctgacc agcccttctc tgggctggtt tttgatttaa gtgctgctgt
    10741 atgactcctt aagtaaaaat gagcaattct tgatatttct ggtcattcac ctcaattaat
    10801 attattccat atcctccggg gacatcttac tataatattt attgatgagt gattatgacc
    10861 aatattaata cagcttgtgt aaaaaataat gccagttatc aacttaataa cgcattacct
    10921 aacaaagaaa ccatctccag caatttttgt gaacgactag aacaatgggg taataaatcg
    10981 gttaataatg gtgaagaaag agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg
    11041 aacatggcat ctcttgattt gtcatatctt gatttgagtg aattaccgcc tatcccctcg
    11101 acagttaata cgttaaatct ggaaaacaat tgtctaactt gtcttgactt agctgacaat
    11161 gccagtctcg ttaatatcaa cctcagcgtt aataaaatta acacgataac ctttccaaaa
    11221 gaaccaaaac tggaaaacat ttatattaat cataacaatc tggaaagttt agatttaaaa
    11281 aatcagcatt cattggttaa tctggaagcg caaaataata gcctaaaaaa atgatatttc
    11341 tgatagttat aaactgaaat ttcttaatct tgattataac aagctagcat cgctggatgt
    11401 ctcccggcaa gaatctctga ttgagttaag tgcccatcac aatatgatca ctgaccttat
    11461 attacacaat catcccagga tgaaaacaat cactttaaac gacaaccata ttgcacattt
    11521 aaacgccaaa aacactacaa aactggaata tttaaactta agcaataaca atttactgcc
    11581 aaccaatgac attgatcaac taatatcatc aaaacatctt tggcatgtat tagttaacgg
    11641 catcaacaat gatccacttg cacaaatgca gtactggact gcagtaagaa atataattga
    11701 tgacactaat gaagtgacca ttgatttatc aggacttaat ttaaccactc aaccaccagg
    11761 gctgcaaaac ttcacctcta tcaatcttga taataaccaa ctcacacatt ttgatgcaac
    11821 caactatgat agactcgtaa agctaagtct gaatagtaat gctcttgagt caataaattt
    11881 tcctcaaggc agaaatgtaa gtattacaca tatatctatg aataataatg ctctcagaaa
    11941 tattgatata gataggcttt catcagttac ttattttagt gcggcacata atcaactaga
    12001 gtttgtgcaa ttagaatctt gcgaatggct gcaatacctg aatctcagcc ataatcaatt
    12061 aactgatatt gttgcaggta ataaaaatga actcttactg ctggatctat cccataataa
    12121 actaacaagt ttacacaatg tcttatttcc caacttgaat acgttactta ttaacaacaa
    12181 tttgctttct gaaatcaaaa tattctatag caacttccgc aatgttcaga cattaaacgc
    12241 tgctaacaac cagttgaaat atataaatct tgatttcctg acttatcttc catctatcaa
    12301 aagtttaaga ctggacaata ataaaataac ccacattgat actaataata catccgatat
    12361 tggaacttta ttccccataa taaaacagag caaaaactta aattttttaa atgtttctgg
    12421 gaagaacaat tgacctacta tgcagctcat gttatttaat ttgttttccc cagcacttaa
    12481 gcttaatact ggcctggcaa ttctttcgcc tggtgcattt gaagttcact ctgacggaat
    12541 agatgcggat aacgaattgt ttcactatac tattaaaaaa gcatataccc catataatat
    12601 acacacttac aagacagagg aagttgtaaa ccagaggaat ataaaagtta aaaacatgac
    12661 ct
//
LOCUS       DAJTSF010000105        12650 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000105 DAJTSF010000000
VERSION     DAJTSF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.105"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1040
                     /locus_tag="OO335_003248"
     CDS             <1..1040
                     /locus_tag="OO335_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6003515.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1041..3206
                     /locus_tag="OO335_003249"
     CDS             1041..3206
                     /locus_tag="OO335_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR6003516.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3220..4131
                     /locus_tag="OO335_003250"
     CDS             3220..4131
                     /locus_tag="OO335_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR6003517.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4131..5426
                     /locus_tag="OO335_003251"
     CDS             4131..5426
                     /locus_tag="OO335_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR6003518.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5471..5701
                     /locus_tag="OO335_003252"
     CDS             5471..5701
                     /locus_tag="OO335_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003519.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5679..6179
                     /locus_tag="OO335_003253"
     CDS             5679..6179
                     /locus_tag="OO335_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR6003520.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6179..7597
                     /locus_tag="OO335_003254"
     CDS             6179..7597
                     /locus_tag="OO335_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR6003521.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7597..8445
                     /locus_tag="OO335_003255"
     CDS             7597..8445
                     /locus_tag="OO335_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003522.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8445..8900
                     /locus_tag="OO335_003256"
     CDS             8445..8900
                     /locus_tag="OO335_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR6003523.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8903..9595
                     /locus_tag="OO335_003257"
     CDS             8903..9595
                     /locus_tag="OO335_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6003524.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9605..10936
                     /locus_tag="OO335_003258"
     CDS             9605..10936
                     /locus_tag="OO335_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6003525.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10937..>12650
                     /locus_tag="OO335_003259"
     CDS             10937..>12650
                     /locus_tag="OO335_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR6003526.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEK"
BASE COUNT         3404 a         3129 c         3334 g         2783 t
ORIGIN      
        1 ctttctttcc gaaccttgtt gatcaccttc tggttaagca tcacacggct gatggcgttg
       61 aagatggcat ttcaatctgc tacttcaagc catactcgca aggccgtgct cgctggcagg
      121 gtgacacaat ccacggcgtg tggtttgacg aagagccacc atacagcatt tatggcgaag
      181 gtcttacccg taccaacaaa tacgggcaat tctcaattct gacgtttacc ccgctgatgg
      241 ggatgtctga cgttgttacc aagttcctga agaaccccag caagtcgcag aaagtggtca
      301 acatgaccat ctatgacgct gagcactata ccgacgagca gaaagagcaa atcatcgcat
      361 catatcccga gcatgagaga gaggcgcgtg ctcgcggtat tcctacgatg ggtagtggtc
      421 gaatcttcca gataccggaa gagacgatta agtgtcagcc gttcgagtgt cctgatcact
      481 tctacgtaat tggcgggatg gatttcggat gggatcaccc acaggcgcaa gttcagcttt
      541 ggtgggataa ggaggcagac acaatctacg tttcacgcgt gtggaaggcg aaagaaaaaa
      601 cagccgttca ggcgtgggga gccgttaaat catgggcgca taaagtgcca accgcatggc
      661 ctcatgatgg aaaccagcac gagaagggcg gcggtgagca gctcaaaggg cagtatgcag
      721 acgctggatt tatgatgttg caggagcatg cgacatggcc tgatggcggt aacgctgttg
      781 agccaggcat cactgaattg cgcgacatga tgctcgatgg tcgcttcaaa gtattcaata
      841 cctgtgagcc attctttgag gagttccgcc tctatcaccg tgatgaaaac gggaaaatcg
      901 tcaagcttaa cgacgacgtg ctatccgccg ttcgctatgc atacatgatg cgccgcttcg
      961 caaaaatgat gcgcgacatc aaaaaaccaa aagagaaaaa gataccagcc ccaatcaggc
     1021 ccatcgcacg gagaacttaa atggccgacg aaaacagact caattccatt ctgtgtaagt
     1081 ttgacgcaga ctggatggcg agcgatgaag ccagaaccga ggcgacaaat gacctgtatt
     1141 ttagccgagt gtcgcaatgg gatgactggc tatcaaacta cactaccctg caatatcgcg
     1201 gacaattcga tgttgttcgc ccggaggtca ggaaactggt cgcagagatg cgccggaacc
     1261 ctgtcgacgt tctcttccga cccaaagacg gcgctaatcc tgatgcagcc gatgtattga
     1321 tggggatgta tcgtactgat atgcgccata acacggcaaa gattgccgtt aacgttggcg
     1381 ttcgtgagca gatagagtca ggcgttggtg catggcgtct ggtcacgcag tacgaagaca
     1441 acgatccaac aagcaacaat caggtaatcc gacgcctgcc aatccatgaa gcctgctcac
     1501 acgtcatatg ggacgccaac agcaagcaga tggataagag cgacgctaag cactgcacgg
     1561 tgattaacgc cttgtcgcgc aatggctgga aagagttcgc agaggattac ggtattgacc
     1621 cggataccct gccatctttc cagaatccta acgatacatg gctattcccg tgggtgtcga
     1681 atgatgtcgt ctacgtcgct gagtattacg aggtagaaga ggagaaggag aaggtcttca
     1741 tctaccgcga cccgctgaca ggtgagccgg tcagctatta ccagcaggat atcaaagacg
     1801 tcatcgacga cctggctaat cgtggattca ttaaggtagc agagcgcaag gtgaagcgtc
     1861 ggcgtgtgta taagtcgatc atcacctgta cgcagatact gaaagaccgc gagaagatag
     1921 ccggagagca tattccaatc gttccagtgt atggcgaatg gtcattcgct ggtgacaagg
     1981 agtgctacga gggtgtggta aggctgacga aagacggtca acgccttcgt aacatgatta
     2041 tgtcgttcaa cgccgatatt gttgctcggt caccgaagaa gaaaccgacc ttcttccctg
     2101 agcaaatcga aggctacgaa tacatgtacg gcggaaatga tgactatccg tactatctgc
     2161 agaacaggac tgatgaaaac ggtaacgacc tgccgattgg tccaatctcc tacatggaaa
     2221 accctgaagt gccgcaagcc aacgcttaca tgcttgaggc tgccaccaac gcagtgaaag
     2281 aagtggctag tcttggcgtg gatgcgcagg cggcaaatgg tcaggtcgct tttgataccg
     2341 tcaatcaact gaacatgcgg gcagaccttg agacatacgt gtttcaggat aacctggcta
     2401 ccgcaatgcg acgtgatggc gagatttatg cctcaatggt caacgatatt tatgacgttc
     2461 ctcgtcatgt aacgctgaca cttgaagatg gaagcgagaa agacgttcaa ctctatgcgc
     2521 aagttgtcga ttaccagtcc ggcaatgtgg tcacactcaa cgacattcgc ggtcgatatg
     2581 agtgctatac agacgttggg ccatccttcc agagtatgaa ggaacagaac cgcgcagaga
     2641 ttcaggagtt gctcaccaag gttccgcaag gtactccaga gttccagatg ctgatgctgc
     2701 aatacttcac gctgcttgac ggtaaaggcg tcgagatgat gcgagagtac gcgaacaagc
     2761 aactggtgat gatggggctg aagaaaccag aaacacctga agagatggag atggtgcagc
     2821 aggcacaaca acagccgcag cagccatcag cagagcaaat tcaggcgcag ggtatccttc
     2881 tgcaaggtca ggctgaattg ctcaaggcag agaaccaaca ggcgcagatt caggttgaag
     2941 ctgccaaggt tgaagcccaa aaccaactca acgccgcgaa gattgcagaa atattcaaca
     3001 atatggacct cgacaagcag gcagaactgc gtgagtacct caggctcgta ggtcaattcc
     3061 agcaacagcg cagcaaagat gctcgtgcta acgctgagct gcttcttaaa gatgcagacc
     3121 agactcattc acaacgcatg gatttcgcga atcttatgcg tcaagttcaa atcccctccg
     3181 gcggagtagc cgagacacct caataagaga gagttaatca tggaccaaac caccgacatt
     3241 caggcttctg aagaattaac cctgcccggc aatcatgcag cggcatctgc tgatggctta
     3301 gttgtcgata atgccaacga cagcgcaggt caggaagaag gctttgagat tgtccggaaa
     3361 gacgatgaga aaccaaaaca agacccggca actaatgctg aatttgcccg tcgccgcatc
     3421 gaacgcaaac gccagcgtga gcttgagcag cagatggaag cggttaagcg tggagagttg
     3481 ccggagcacc tgcgggtgaa ccctgagtta ccaaaacaac cagaccctaa cgattatctt
     3541 tccgaagatg cactggctaa gtacgactat gaccagagcc gcgcactggc tgccttccag
     3601 caggcaaaca gtgaatggca gatcaaggct atggacgcac gaagccaggc tgtcgccgag
     3661 cagggtcgca aaactcagga gttcacccag caatcagcgc aatacgtcga ggcggcccgt
     3721 aagcactacg aggcagcgga aaagctcaat atccctgact atcaggagaa agaggatgca
     3781 ttcatgcaac tggtgccgcc agcagtcggt gccgacatca tgcgcctctt cccggagaaa
     3841 tccgccgctc tcatgtatca ccttggtgct aatcctgaga aaacacgcca gttgctggcg
     3901 atggacgggc aatccgcgct gattgaactc actcgactgt cagaacgttt aactctcaag
     3961 cctcgagcca agcctgtttc agaagccccg ttacctgatg aacccattca gggacatgct
     4021 gttgctgcaa atatctctgc gattgaaaag cagatggagg cggcagcaaa caaaggggat
     4081 gtagagacgt accgcaagct caaggcgcaa ctgaataaag gaattcgata atggcattaa
     4141 atgaaggtca actggtcacg tatgctctgg atgaaatcat cggaaccgtc cagaacctga
     4201 cgccaatggc gtcaaaagtg acaaaatacc cccctccggc agaatccatg cagcgttcaa
     4261 gcaacaccgt gtggatgcct gttgagcagg aagcgccaac ccagactggc tgggatttaa
     4321 ctggcaacgc aaccgggatt ctggaactct ccgtgaagtg caacatgggc gatccggata
     4381 acgatttctt cgagcttcgt gcagatgacc tgcgtgatga gcgttcttac cgtcgccgca
     4441 tccaggcatc cgccaaaaaa ctggcgaata acattgagtc agcgattgcc aaacaggcaa
     4501 ctgaaatggg ctcgcttgtt gttcacgata cccgcgcaat tggtccatcg actggcctgc
     4561 ctggctggga ttttgtgtct gatgcagagc gactgatgtt ctcccgtgag ctaaaccgcg
     4621 acatgggcat cagttacttc ctgaaccctg acgattaccg caaagcaggc cgcaacctgg
     4681 tagatggtga catcttcggg cgcgttcctg aagaagcata tcgaaacggt actattcagc
     4741 gtcagattgc tggctttgat gaaattcttc gctcaccgaa acttccggca gttaccaagt
     4801 caaccgcgac tggtgtaact gtttctggtg cgcagaagtt taagccgcag gcatacaccc
     4861 ttgataccga tggtaacaaa gagaacgtcg acaaccgtgt tgcaacggtg accgtatcct
     4921 ccaccaccgg atttaagcgc ggcgacaaaa tcagcttcat tggtgtgaag ttcctgtctc
     4981 agatggcgaa gaatgtgctg actgatgacg cgactttctc aatcacccgt gtgatcgatg
     5041 gtactcacat cgaaatcacg ccgaagccga ttgcgctgga tgacgcgtca ctgacaaaag
     5101 aagagaaggc ttacgctaac gtaaacacct ctcttgctga taccactccg gtaaacgttc
     5161 tgaacgtggc aacaaccacc gctaacgtgt tctgggctga tgactcaatc cgcctgctgt
     5221 ctcagccgat cccggtaacc catgaactgt ttgctggcat gaaaacgtct tccttcagca
     5281 ttccaggtat tggtgttaac ggcatcttcg caacgcaggg tgatatcaac actctgtctg
     5341 gtaagtgccg tattgctgtg tggtattcag catgtgctgt acgaccagag gcaattggtg
     5401 ttggtctgcc taaccagacc gcgtgataac cagagggagc ttcggctccc gtttttatct
     5461 ggagacaagc atgacacaca tgatctttcg tcatggcgac atgaagaagt ggaaaggcgt
     5521 tggctacgac tttgaaatcg tgaaagccga agagcttcag gaatatctgg atgctggctg
     5581 gttttcacat cctgatgacc ttttgaagga tgttgcagag ccagagccag aagaaaaaca
     5641 gcgtaaaaag cctggtcgaa aacctaaggc ggcagcagat gaacctgaca acgaagggtg
     5701 atttagttct tgcggcatta cgtaagctcg gtgttgcatc aaatgccacg ttaaccgatg
     5761 tcgaaccgca gtctatggaa gacggcgtca acgaccttga aatgatgatg gctgaatggc
     5821 ttggcggtga tgcgtcacct ggtatcaacg ttggctacat ttttgctgat gcagatgttg
     5881 ctccggatcc gggcgatgag cacggtttat caaataacgc tatcaatgcc gtcattttca
     5941 accttgcctg ccgaattgct ccagattatg cgctggaagc gtctgcaaaa cttataacca
     6001 ctgccagata cgggaaagag cgactcgtca aactgtctgc aatggacagg gcaaaagcag
     6061 ctaaatgtaa gtccggttat ccaaaccgta tgcctgttgg tagtggtaac cagttggcga
     6121 agtggaatgg ttggaattac ttccaccgaa aggaaccttg cgataacggg agcgaataat
     6181 gcctattcag caacttccgc ttatgaaagg tgtcggcaaa gacttccgaa acgccgacta
     6241 tatcgactat ctgccagtga atatgctggc tacacccaaa gaaatcctga acagcagcgg
     6301 atatcttcgc tcattcccgg gcattgccaa acgttctgac gtgaacggtg tatctcgcgg
     6361 cgtcgagtac aacatggcgc agaatgctgt ttatcgcgtg tgtggcggca agctgtacaa
     6421 aggagaaagt gaagtcggtg atgttgccgg aagtggtcgt gtatcaatgg cgcatggtcg
     6481 gacatcacag gcggtaggcg ttaatggtca actggtcgag tatcgctatg atggcacggt
     6541 caaaaccgtc tcaaactggc ctacagacag tggattcact cagtatgagt taggttcagt
     6601 tcgcgacatt acgcgcttac gtgggcgtta tgcgtggtca aaagacggaa ctgattcatg
     6661 gtttatcact gaccttgaag acgaatctca tcctgaccgt tacagcgcac aatatcgtgc
     6721 cgagtctcag cctgacggca tcatcggtat cgggacatgg cgagacttca tcgtctgctt
     6781 tggttcatcg actattgaat atttctccct gactggcgca accaccgttg gtgccgcttt
     6841 gtatgttgca caaccatcgc tgatggtgca gaaaggcatt gccggaactt actgcaaaac
     6901 gccattcgct gattcgtatg cgtttatcag caatccggca acgggtgcgc cgtctgtgta
     6961 tatcatcgac tccggtcagg tgtcaccaat tgccagcgcg agcattgaga aaatcctccg
     7021 ctcctacact gctgatgaac tggctgatgg tgtgatggaa tcgctgcgat ttgatgctca
     7081 tgagttgctg attatccacc ttccgcgtca cgtcctcgtg tacgacgcat cttcaatcgc
     7141 caatggccca caatggtgtg tgctgaaaac aggtctgtat gacgatgtgt accgcgctat
     7201 cgacttcgtt tacgaaggca atcagataac gtgcggcgat aagctggaat ctgttaccgg
     7261 gaaactgcaa ttcgatatca gcagccagta cgacaagcaa caggaacacc tgctgtttac
     7321 tccactcttc aaagcggata acgccagagt gttcgacctt gaggttgaat cgtcaactgg
     7381 cgttgcgcag tacgccgacc gcctttttct ctctgcaacc actgactgca tcaattacgg
     7441 gcgtgagcag atgattgagc agaatgaacc gttcgtttac gacaagcgtg ttttgtggaa
     7501 gcgagtaggg cgcatcagga aaaatgtcgg cttcaaattg cgcgttatca cgaagtcacc
     7561 tgtcactctg tctggcgctc agataaggat tgagtaatgg ctgattcgaa tctcaatgtg
     7621 ccggtaatca tccaagctac gcggctcgat acatcagtcc ttccacgcaa tatcttctcg
     7681 cagtcgtatc tgctttacgt tatcgcacag ggcactgatg ttggtaacgt ggctaacaaa
     7741 gccaacgagg ccggacaggg cgcttatgat gcacaggtca ggaacgatga gcaggatgtg
     7801 attctcgctg accatgagca gcgaatttct gctgcggaag caacgcttgt taatcatgag
     7861 gagcgaatca gacaggcaga atcaactctt caggaccatg aaacacgaat agctcagaat
     7921 gaaagcgata tttcgtcgct tgacacaaga gttcagtcgc tggagtcgca ggtttcagac
     7981 catgaaacgc gcatcgatgc tctggagtat gcaaccacac gcaagaagtc agaggttgtt
     8041 tactctggtg tatctgtaac catcccgaca gcgccgacca accttgttag cctgctgaaa
     8101 acgctcacac cgtcatccgg ctcgttggca ccattcttcg acaccgttaa caacaagatg
     8161 gttgtgttca acgagaacaa aaccctgctc ttcaagttgt cgattgtcgg cacgtggccc
     8221 agcggaaccg ccaacaggtc aatgcagcta accttttccg gctctgttcc tgacacactg
     8281 gtcagcagtc gtaatgcggc gacaacaacc gacaacatcc tgttagctac gttcttcagc
     8341 gtggataaag acggctttct tgccacaaat ggcagtacgt taaccattca gtcgaatggt
     8401 gcggcgttta ctgccacaac catcaagata atcgcggagc agtgatgatt acattcaaac
     8461 caacgcgaaa catcgacctg atagaagcag tcggaaatca ccctgacatt atcgccggga
     8521 gcaacaacgg tgatggatac gactacaaac ctgaatgccg ttactttgag gtgaacgtgc
     8581 acggtcagtt tggcggcatt gtttactatc aggagattca gccgctgaca ttagattgcc
     8641 acgccatgta cctgccagag attcgcggct tcagcaagga aattgggctg gcgttctggc
     8701 gatacattct gactaacacc accgttcagt gcgtaacatc gttcgctgca cgcaaattcc
     8761 gacacggtca gatgtactgc gcaatgattg gccttaagcg tgtaggaacc atcaagaaat
     8821 acttcaaagg cgtggatgac gtgacttttt acagcgccac acgcgaagaa ctaatcgact
     8881 tcctgaatca cgggagatag ccatgttata tgcatttaag ctgggcagaa aactgcgcgg
     8941 cgaggaacct tattgccctg aaaaaggcgg gaaaggtggc agttctgata aaagcgcaaa
     9001 gtatgcagca gaagcccaga agtatgctgc agacctgcaa aatcagcagt ggcagacgat
     9061 catgaaaaac cttgctccgt tcacgcctct tgcggagcag tatgttaacc agcttcagaa
     9121 cctttccagt ttagaaggtc aggggcaggc acttaatcag tattacaact ctaagcagta
     9181 taaagacctt gcagggcagg cgcgttacca gagtcttgct gctgcggagg cgacgggtgg
     9241 acttggttcg acagccacaa gcaatcaact ggctacgatc gcgccgacac tcggtcagtc
     9301 ttggttatca aatcagatga gcaattacaa caatctggca aacgttgggc ttggtgcgct
     9361 gcaaggtcag gcaaacgccg ggcagacgta cgccaacaac atgagcagca ttgcacagca
     9421 aagcgcagct cttgccgctg ctaatgccaa taaaccatca agtcttcaga ctgcaattag
     9481 cggtggcacg tctggtgcga ttgccggtgc aggtcttgcc agccttttgg gaacatcaac
     9541 gccttggggc gctggtattg gtgctggtat cggattgctt ggctcgttgt tttaaggggg
     9601 aatcatggct acttggcaag gaacaaacgg cggattgttg gctggtatcg gcggcgtcaa
     9661 ctcaaacgct ccgagcgtaa atgacatcgg caatacgctt cagcttatca ggcagaacaa
     9721 tgatattgag cgttcaggcg ctaacaatgt tgggctgact gctttgcaag gtctttcagg
     9781 tattgcgggt gtttttcagc aggaaaagca ggctcagcgg cagaaagaat ttcagcaggc
     9841 gtacgctaat gcttatgcgt ctggtgatcg cggtgctttg cgtcagttgg ctactcaata
     9901 tccagaccag attgaatccg ttcgtaaagg catgggattc attgatgaag agcagcgtaa
     9961 ttctatcggc accttagcgg ctggcgcacg ccttgcgtca tcgtctccag aagcaatgca
    10021 atcatggctg caaaacaacg ccggtgagtt agctcgtgtt ggcgttaatc ctcatgacgt
    10081 cgctcagatg taccaacaga acccgcggca gttcggcgaa tttgtcgatc acctggggat
    10141 gaacagtctc gggcccgaaa aatactttga cctacaggat aaaatgcagg gtcgccaggt
    10201 taccatgcgc ggtcaggatc tggattcgca aaccgccgct cggaatcagg caatcacaat
    10261 gcgcggacaa gatatccagg cgaatttagg tcagcagcgc attaatctgg acgcagaaac
    10321 aaaccgcatt aacaacgaaa ataagcgcct tgaccggatg ctatcagcag aaactaacga
    10381 cctgaagcgc caggaaatac agagccgcat agcagccaac aaccagcagt tgcagcagaa
    10441 gcagcaagcg ctaaatgatg gctacaaaga cggcatcaac accctcacaa ccagcatgtt
    10501 cactctgaac gatatcgtta gttctccttc acttaagagc attacaggct tacgtggagt
    10561 aatccccaac gttccaggct cacaggctgc agacactcag gcacgacttg atacctttaa
    10621 atcacaagca tacctgacag cggttcaggc catgcgaggc atgggcgcac tttctgatgc
    10681 cgagggcaaa aagctcgacc aggctgttgg ttcgctgcag aactcgcaga gcgaggagtc
    10741 ctttcgtcgc aacgctggcg tcatcctgaa cacgctcaac cagaagcgta atgaggcggt
    10801 tggtaagtac gttcagcaaa acggtatcaa gcgagtggaa gcgcctcagg cttctataga
    10861 ttacctgaag cagcaccccg agctgtcaat cgacttcatt aatcgctacg gatatcttcc
    10921 atctttgggg cagtaaatgg ctaattaccg tgatttgtta gagcaggctg gcgcacgtta
    10981 cggtgtgcca gaagggttga tgactgcact gggtgccaag gagtcttctt acaaccctgc
    11041 cgcagtaagc tccgccgggg ctgtaggatt gactcaggtc atgcctggga catggcgtga
    11101 tatgggttat accgatgagc aaatgcaaaa ccccgaatat caggctgacg ctggcgcgcg
    11161 ctatctggca aagatgtacc agcagtttgg taactggcgt gactctcttc aggcttatca
    11221 cgacggtccc ggcaacgtta tgaaggcaaa gcgtggtgaa tatacgccag gacctgaagg
    11281 ccgcggttac gttgatgatc gctttgctca atgggctggt gacccggtga cagactcaac
    11341 agtcgaacag cgcgccacct ctgcaaaggt acatcctcag caagacccta acaacccgtt
    11401 tgcacaactg gaagtacagt catccgaaca agtatcggca tcaggtgtgc agtcagaccc
    11461 aaataatcca tttgctcaga ttgagcagca ggcagccagt cagcagccac ctcaacccgt
    11521 aagttctgtc gcaccgaaac ctgttcagca acaaacagtt aatcaggcca ataatgcacc
    11581 aacacgggaa gaaccatcat tgatgcaaca agctggcgat tggctcactg gtggtcaaag
    11641 tgcagggcaa attgcagaac aggctggtcg tggtctggta aacataccat ttgacgtatt
    11701 gcagggtggc gcaagtctga ttaatgcaat cagtcagggg cttggggggc caaaagtatt
    11761 ggatgatgtt tatcgtccag tagacagacc gacagacccc tacgcgcaag ctggagagtc
    11821 aataggcggt tatcttgttc ctggagcagg tgtagctgga aacatggtca ttggttctct
    11881 cgctgacgcg gcgaatcaac ggggtgattt tgccgaaaat gccgctatta atgccggact
    11941 tagcattgct acgcatggcc tgataaatgg cgttacccgt ggtgttcgtg gtgcatcaaa
    12001 tataattagt ggcaataaaa catctgcaca gagagcgacc actgcgccaa cagaaacatc
    12061 accattctcc ggtgatgccg ctgcagcaac aaatcctgcg gttcatgccg cagaggcaag
    12121 agtagcacaa ggtgtaccaa tgacgcctgc gacgaagaac ccagaggaag tcgttcgcac
    12181 agtagcagca caaaaaaggc caaatctcgc ttcatcgctt gatgaactag atatcaatcc
    12241 tcaggctgaa gttctggagt ctgctgaaag gcttaatgtt gattcattac tcccttcaca
    12301 cttttccggg aacgagcaat acaaggcagt tgagcaagca atcaagtccc gtgcgggttc
    12361 tgctctacag gtgcaggaaa atgaagcaat caggcagcta gcacagggcg cgggtgagat
    12421 aattgatcgc gtttccggtg caaaagatgc tcttggtatg agcgacaagt ttattgatac
    12481 ggtcaatgga agaatgtctg cgctgatgaa acgaagcgac cagctttatc gcaatgttga
    12541 aaaggcgatg cctgcaggtg caaaaattga tgcgccatca acaaggtcaa tgctcaaaca
    12601 ggtggcagaa gatcttggcg ggatgaaaaa ccttgaccct attgaaaaga
//
LOCUS       DAJTSF010000106        12648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000106 DAJTSF010000000
VERSION     DAJTSF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.106"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            758..841
                     /locus_tag="OO335_003260"
     CDS             758..841
                     /locus_tag="OO335_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6003527.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="OO335_003261"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="OO335_003261"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6003528.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="OO335_003262"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="OO335_003262"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6003529.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="OO335_003263"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="OO335_003263"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6003530.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="OO335_003264"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="OO335_003264"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6003531.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="OO335_003265"
                     /pseudo
     CDS             complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="OO335_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="OO335_003266"
     CDS             complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="OO335_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6003532.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8830..9165
                     /gene="uspB"
                     /locus_tag="OO335_003267"
     CDS             8830..9165
                     /gene="uspB"
                     /locus_tag="OO335_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6003533.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="OO335_003268"
     CDS             complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="OO335_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6003534.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10967..12166
                     /locus_tag="OO335_003269"
     CDS             10967..12166
                     /locus_tag="OO335_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6003535.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3112 a         3170 c         3359 g         3007 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     6421 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
     6481 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
     6541 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg
     6601 tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt
     6661 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
     6721 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
     6781 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
     6841 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
     6901 tatccagctt gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca
     6961 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
     7021 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
     7081 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
     7141 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
     7201 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
     7261 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
     7321 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
     7381 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
     7441 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
     7501 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
     7561 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
     7621 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
     7681 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
     7741 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
     7801 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
     7861 ttttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
     7921 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     7981 gaacacgtcg tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc
     8041 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg
     8101 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     8161 gcctaggtcg ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag
     8221 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     8281 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     8341 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     8401 gagatcgact gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     8461 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     8521 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     8581 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     8641 atattatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
     8701 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
     8761 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
     8821 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
     8881 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
     8941 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
     9001 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
     9061 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt
     9121 ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa
     9181 taacgtataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca
     9241 ggaactgcaa ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa
     9301 acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca
     9361 ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat
     9421 aactcgccag gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg
     9481 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
     9541 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga
     9601 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac
     9661 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
     9721 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
     9781 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
     9841 ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg
     9901 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
     9961 cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca
    10021 acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga
    10081 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    10141 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    10201 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    10261 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    10321 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    10381 atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    10441 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    10501 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    10561 ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    10621 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    10681 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    10741 gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca
    10801 aagtgaaata tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt
    10861 atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg
    10921 aggaatcttt acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga
    10981 tgccattatt ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt
    11041 aggacgccgg gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc
    11101 tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca
    11161 gaatccgcat ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct
    11221 ggtcaataaa cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga
    11281 ctccgcgcag cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt
    11341 cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct
    11401 gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc
    11461 ggggctgggc gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct
    11521 gccgacccgc gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca
    11581 ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg
    11641 tgagaactta ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag
    11701 ctactggcaa ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac
    11761 cttcctgaat gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt
    11821 tcatctaccg aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc
    11881 gctaaaacag ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg
    11941 cgtacaaccc aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga
    12001 caccaacgaa ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat
    12061 cggtgaagtg atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc
    12121 gagtgcatgg gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc
    12181 cgtcagccgt ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt
    12241 ctggtgttaa ctcgctgttt tgttagcgaa ttaacgctat tgccgttgtg gttattttat
    12301 agtgtattta ttctcagaga atatatatta ctgaaataaa aaacacaaag aataaccaaa
    12361 ggaaattatt tgaaacgtaa aaggtatata tttttaatct gtaaaattta aaatcacctc
    12421 ctctggattg aatcaacttg ctctatttaa gacgattgtt tttacatttt caccacctgt
    12481 atcaggtggt gtttttttat tggcggcaat gcctttatct tatttttatt acgttgtaaa
    12541 tatggctatt cctaaatgaa atttgatttg ctcagtttat ttgtgatgtc aggggagtgc
    12601 ttgccatgag agtcgtgata ttattattca tcatttttag gtaatgct
//
LOCUS       DAJTSF010000107        12536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000107 DAJTSF010000000
VERSION     DAJTSF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.107"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..983
                     /locus_tag="OO335_003270"
                     /pseudo
     CDS             1..983
                     /locus_tag="OO335_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1231..1306)
                     /locus_tag="OO335_003271"
     tRNA            complement(1231..1306)
                     /locus_tag="OO335_003271"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1271..1273),aa:Ile,seq:cat)
     gene            1865..4118
                     /locus_tag="OO335_003272"
                     /pseudo
     CDS             1865..4118
                     /locus_tag="OO335_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4454..5386
                     /gene="csiD"
                     /locus_tag="OO335_003273"
     CDS             4454..5386
                     /gene="csiD"
                     /locus_tag="OO335_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR6003536.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            5406..6674
                     /gene="lhgO"
                     /locus_tag="OO335_003274"
     CDS             5406..6674
                     /gene="lhgO"
                     /locus_tag="OO335_003274"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR6003537.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            6697..8145
                     /gene="gabD"
                     /locus_tag="OO335_003275"
                     /pseudo
     CDS             6697..8145
                     /gene="gabD"
                     /locus_tag="OO335_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            8153..9439
                     /gene="gabT"
                     /locus_tag="OO335_003276"
                     /pseudo
     CDS             8153..9439
                     /gene="gabT"
                     /locus_tag="OO335_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            9677..11077
                     /gene="gabP"
                     /locus_tag="OO335_003277"
     CDS             9677..11077
                     /gene="gabP"
                     /locus_tag="OO335_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR6003538.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            11098..11760
                     /gene="csiR"
                     /locus_tag="OO335_003278"
     CDS             11098..11760
                     /gene="csiR"
                     /locus_tag="OO335_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR6003539.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(11761..12210)
                     /gene="kbp"
                     /locus_tag="OO335_003279"
     CDS             complement(11761..12210)
                     /gene="kbp"
                     /locus_tag="OO335_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6003540.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(12294..12452)
                     /locus_tag="OO335_003280"
     CDS             complement(12294..12452)
                     /locus_tag="OO335_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6003541.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         3205 a         3088 c         3172 g         3071 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
      421 tcagcccggt cagctaccgc gcacacggcc tgcctgtgtc agaaaatctg ttggagcagg
      481 atttttacgc cagtggcccg aaccagaagt ggccaggaga catcacgtac ttacgtacag
      541 atgaaggctg gctgtatctg gcagtggtca ttgacctgtg gtcacgtgcc gttattggct
      601 ggtcaatgtc gccacgcatg acggcgcaac tgccctgcga tgccctgcag atggcgctgt
      661 ggcggcgtaa gaggccccgg aacgttatcg ttcacacgga ccgtggaggc cagtactgtt
      721 cagcagatta tcaggcgcaa ctgaaacggc ataatctgcg tggaagtatg agcgcaaaag
      781 gttgctgcta cgataatgcc tgcgtggaaa gcttctttca ttcgctgaaa gtggaatgta
      841 tccatggaga acactttatc agccgggaaa taatgcgggc aacggtgttt aattatatcg
      901 aatgtgatta caatcggtgg cggcggcaca gttggtgtgg cggcctcaat ccggaacaat
      961 ttgaaaacca gaacctcgct taggcctgtg tccatattac gtgggtagga tcaatttgcc
     1021 cgcttggcat agcgggcatt ttttccaggt acttttgaat gagtactgat gaataaatac
     1081 attgcagtgg cgtgccacgt accaaaacac cagccctcat tcgaaaccac ccaccgcact
     1141 tcttccttga aatggcgtta gtcatgaaat atagaccgcc atcgagtacc ccttgtaccc
     1201 ttaactcttc ctgatacatg aatgatgatt tggtggccct tgctggactt gaaccagcga
     1261 ccaagcgatt atgagtcgcc tgctctaacc actgagctaa agggccttaa gtatgcaata
     1321 acaatactta taaaccacgc aataaacatg atgatcttat gatgtaaata acagattttt
     1381 atgcgttccc attcaccttg ggtcgtgatt acacgcgaca taaaacccga caccgcctcc
     1441 attcgcaaag tcgatactcg cagtcaacaa gcaaatgtta ataattagca ctatctatag
     1501 ttatcatcga ttcaatgata ctttgtaatg attttgtatc taataatata actttattac
     1561 attagctgaa gagttttcgc atcattatga tatctgttac ttttcactcc ataaaaataa
     1621 acttcatata gcaatatatt ctttcataga tcttattctg ctaatcatta gtttcgtatg
     1681 cgcgattttt gacagttgcc tctccagacc acatcgataa ttaaaaaaca gatttaaaca
     1741 ttatcctttt ccatatagat attggataaa aataggacat ctgtttgcaa ttactttcac
     1801 aacaattaaa catttttatg ttttcgtata catcatatta ctctaccatt agaggaacgt
     1861 tattatgttt tctataaaac caggacctgg aaatttacct atcgacaacc ccacattgtt
     1921 atcatggaac attactgatg gggatctaaa ttccaaattt aatacattag aatatctaaa
     1981 ctgtataaca aatattatta atgcttgtgg agtttatcct caggatttaa aagacagaga
     2041 aattatatca acttttcacg cagaaaaagt cattaatgat ctgttaaaaa acgattataa
     2101 aatttccctt tctccagata caacttaccg agagctgaat aaagctgcac agagtagcat
     2161 tacagcgcca gacaggatag gagaagggaa aacatgggtt tatcaacgag atacaatggt
     2221 tgaaagaggt gataacagtg gtgttcatca gtatggtcca gctgaacatt tcactcacat
     2281 tatatctgac aaaccgtccc caaaagataa gtatgttgca tatgctatta acattcctga
     2341 ctatgagcta gcagccgatg tatataatat taacgtgacg tcaccttccg gacagcaaga
     2401 aacatttaaa atactaatca atccagaaca tctacggcaa acacttgagc gtaaatctct
     2461 tactgctgtt cagaaatcac aatgtgaaat catcaccccc aaaaaacctg gcgaagcgat
     2521 tcttcatgct tttaatgcca cctaccagca aatcagggaa aatatgtctg agtttgcacg
     2581 ttcccattat gggtatatac aaatccctcc agtgacaact ttccgcgccg acggaccaga
     2641 aactcccgaa gaagaaaaag gttactggtt tcacgcttat caacccgaag atctttgtac
     2701 catccataat ccaatggggg atttgcagga ttttatcgca ttggttaaag atgctaaaaa
     2761 atttggtatc gatatcattc ctgattatac ctttaacttt atgggaatcg ggggaagtgg
     2821 taaaaatgac ctggattatc cctctgctga tatacgagcg aagatcagta aagatataga
     2881 aagtggtatc cctggctatt ggcaaggtca ggttttgatt ccattcatta aagatccagt
     2941 aacaaaagaa cgtaaacaaa tccatccaga agatatacat ctcactgcaa aagacttcga
     3001 agcaagtaaa gataacatct ctaaggatga atggaaaaac ctccatgcat taaaagaaaa
     3061 gagtttaaat ggaatgccta aaacaacccc caaaagtgac caggttatta tgttgcaaaa
     3121 tcaatacgtt cgtgaaatgc gaaaatatgg cgtacgaggt ttacgttatg atgcagcaaa
     3181 acactcaaaa catgaacaaa tagaaagatc aataacccca ccgcttaaaa attataatga
     3241 gcgattacac aatactaact tatttaaccc aaaatatcat gaaaaaaccg ttatgaatta
     3301 catggaatat ctggtaactt gtcagttgaa tgaagaacaa atgtcatcgc tgctttatga
     3361 aagagatgat ttaagcgcca ttgatttttc attgctcatg aagacgataa aagccttttc
     3421 attcggggga gatctcctaa cccttgcatc aaaaccgggt tccacaatct caagcatccc
     3481 atcagaaaga cggatattga ttaatcatta accacgattt tcctaacaat ggcaatcttt
     3541 tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca tatatggccg
     3601 ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa tcaacgactg
     3661 aaattaaaaa ttatgatggg cccacgcgtg tcggtggtga ggcgtggctt aataaaggtt
     3721 gctctaccta tcagcagctc tacaatgaat tccacgcatt atatatagat aaagcaggaa
     3781 tatggagcgc atttgagggt gtatttgcaa ctaaaaacgt tctggccttt agtcgtgggg
     3841 attctgtgaa cattaatcac tctcctcatg atggactagt tataataaat aaaggaaacg
     3901 aagaagttga aggtacctgg cctaacaaat tgcaacctgg aatatataaa aacatgggga
     3961 gtaatagcgt taacattatt attaataata cccgaaaaat tatcccccct tgtaaagtat
     4021 ttacgcttag aggcggatct ctaaatatca atattcctag gcgtagcgct cttcttttag
     4081 ggaaaacagg agaaccgccg aactatctct atttataatt taatgttata tctgccccga
     4141 taaaacgggg cagataatat gtttagttta ctaacggtca ttttgtagtg aagccattta
     4201 ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa cctgttgctt
     4261 tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt tactaatttg
     4321 ttgcttttga tcacaataag aaagcaatat gtcgcttttg tgcgcatttt tcagaaatgt
     4381 agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac atctcataag
     4441 aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg attcaggcca
     4501 ggactatagc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc tggaactcac
     4561 cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc ccgtgcaggc
     4621 gctggagtac aaatcgtttc tgcgttttcg ggtaggcaaa attcttgacg atctgtgtgc
     4681 gaatcagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag gtgcgctgtt
     4741 gatcaatgcg gtaggtatcg atgatgtggc gcaggcagat gagatggtga agctggcaac
     4801 ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc agtattacgc
     4861 gcgtttcgtg gtgaaaaatg tcgataactc agacagctat ctgcgtcagc cgcaccgcgt
     4921 gatggaactg cacaacgacg gcacgtatgt cgaagagatc accgattacg tgctgatgat
     4981 gaaaatcgat gactgggaac atctggacca ctatttccgc catccactgg cgcgtcgccc
     5041 gatgcgcttt gccgcgccgc cgagcaaaaa cgtcagcaaa gatgttttcc atccagtgtt
     5101 cgacgttgat caacagggtc gcccggtgat gcgctatatc gaccagttcg tccagccaaa
     5161 agattttgaa gaaggcgtgt ggttgagcga gctttccgat gccattgaaa ccagcaaagg
     5221 cattctttct gtacccgttc ccgttggcaa attcctgttg ataaacaacc tgttctggct
     5281 gcacggtcgc gaccgcttta ctccgcaccc ggatctgcgc cgtgaactga tgcgtcagcg
     5341 tggctatttc gcttacgcca ctcaccacta ccagacgcat cagtaagcgc aaaggaattg
     5401 agcggatgta tgattttgtg attattggcg gcggcatcat cggcatgtcg accgccatgc
     5461 aactgattga tgtttatccg gatgcccgca ttgcgttgct ggaaaaagag tccggcccgg
     5521 cctgtcacca gacgggccac aacagcggcg tgatccatgc cggggtctat tacacgcccg
     5581 gcagcctgaa ggcgcagttt tgcctggcgg ggaaccgtgc caccaaagcc ttttgcgatc
     5641 aaaacggcat tcgctatgac aactgcggca agatgctggt cgccacgtct cagctcgaaa
     5701 tggaacggat gcgcgcctta tgggaacgca cagcggcgaa cggtatcgag cgcgagtgga
     5761 taaacgccgt ggaactgcgc gagcgcgaac cgaatatcac cgggcttggt ggcatttttg
     5821 tgccgtccag cggcattgtc agctaccgcg aagtgacggc ggcaatggca aaaatcttcc
     5881 aggccagagg cggcgagatt atttataacg ccgaagtcag cgccctcagt gagcataaaa
     5941 acggcgtggt gatacgcacc cgtcagggcg gtgaatatga agcatcgacg ctgattagct
     6001 gttccgggct gatggctgac cggctggtga aaatgctcgg cctcgaaccg ggctttatta
     6061 tctgcccgtt ccgtggcgag tatttccgcc ttgcgccgga gcataaccag attgttaacc
     6121 acctgattta ccccattccc gaccccgcga tgccattttt gggcgtgcat ctcacccgca
     6181 tgatcgacgg cagcgtgacc gtcgggccaa acgcggtgct ggctttcaaa cgcgaaggct
     6241 atcgcaagcg cgacttctca tttagtgaca cgctggagat tttgagctcg tcggggattc
     6301 gccgggtgct gcaaaaccat ctacgctcag gactgggcga gatgaaaaac tcgctgtgca
     6361 aaagcggcta tctgcggctg gtgcaaaagt attgtccccg gctttcgtta agcgatctcc
     6421 agccctggcc cgccggtgtg cgggcgcagg cagtatcgcc ggacggcaag ctgattgacg
     6481 attttctgtt tgtcaccacc ccacgcacga tccacacctg caatgcgccc tccccggcag
     6541 cgacatcagc aattcctatt ggtgcgcata ttgtcagcaa ggtacaaacg ctgttggcaa
     6601 gccagagtaa ccccggacgc acgctgcgag cggcacgtag tgtggatgcc ttacacgccg
     6661 catttaatca ataacctttg aaaacaggac gtaacaatga aacttaacga cagtaactta
     6721 ttccgccagc aggcgttgat taacggggaa tggctggacg ccaacaatgg tgaagtcatc
     6781 gacgtcacca atccggcgaa cggcgacaag ctgggtagcg tgccgaaaat gggcgcagat
     6841 gaaacccgcg ccgctatcga cgccgccaac cgcgccctgc ccgcctggcg tgcgctcacc
     6901 gccaaagaac gcgccaccat tctgcgcaac tggttcaatt tgatgatgca gcatcaggac
     6961 gatttagcgc gcctgatgac cctcgaacag ggtaaaccgc tggctgaagc gaaaggcgaa
     7021 atcagctacg ccgcctcctt cattgagtgg tttgctgaag aaggcaaacg tatttatggc
     7081 gacaccattc ctggtcatca ggccgataag cgcctgattg ttatcaagca gccgattggc
     7141 gtcaccgccg ccatcacgcc gtggaacttt ccggcggcga tgattacccg taaagccggt
     7201 ccggcgctgg cggcaggctg caccatggtg ctgaagcccg ccagtcagac gccgttctct
     7261 gcgctggcgc tggcggaact ggcgattcgc gcgggcattc cggctggcgt gtttaacgtg
     7321 gtcaccggtt cggcaggcgt ggtcggtaat gaactgacca gcaacccgct ggtacgcaaa
     7381 ctgtcgttta ccggttagac cgagattggc cgccagttaa tggaacagtg cgcgaaagac
     7441 atcaaaaaag tgtcgctgga gctgggcggc aacgcgccgt ttatcgtctt tgacgatgcc
     7501 gatctcgata aagccgtgga aggcgcgctg gcctcgaaat tccgcaacgc cgggcaaacc
     7561 tgcgtctgcg ccaaccgttt atacgtgcag gacggcgtgt atgaccgctt tgccgaaaaa
     7621 ttacagcagg cggggagcaa actgcacatc ggcgacgggc tggagaaagg cgtcaccatc
     7681 gggccgctga tcgatgaaaa agcggtagca aaagtggaag agcatattgc cgatgcgctg
     7741 gagaaaggtg cgcgtgtggt ttgcggcggt aaagcgcacg aacgcggcgg caacttcttc
     7801 cagccaacca ttctggtgga cgttccggcc aacgccaaag tgtcgaaaga agagacgttc
     7861 ggccccctcg ccccactgtt ccgttttaaa gatgaagccg atgtgattgc acaagccaat
     7921 gacaccgagt ttggtcttgc cgcctatttc tacgcccgtg atttaagccg cgtcttccgc
     7981 gtcggcgagg cgctggagta cggcatcgtc ggcatcaata ccggcattat ttccaatgaa
     8041 gtggccccat tcggcggcat caaagcctcg ggtctgggtc gtgaaggttc gaagtatggc
     8101 atcgaagatt acttagaaat caaatatatg tgcatcggtc tttaactgga gaatgcgaag
     8161 gaacagcaat aaagagttaa tgcagcgccg cagtcaggcg attccccgtg gcgttgggca
     8221 aattcacccg attttcgctg accgcgcgga aaactgccgg gtgtgggacg tttaaggccg
     8281 tgagtatctt gatttcgcgg gcgggattgc ggtgctcaat accgggcacc tgcatccgaa
     8341 ggtggtggcc gcggtggaag cgcagttgaa aaaactgtcg cacacctgct tccaggtgct
     8401 ggcttacgag ccgtatctgg agctgtgcga gattatgaat cagaaggtgc cgggcgattt
     8461 cgccaagaaa acgctgctgg ttacgaccgg ttccgaagcg gtggaaaacg cggtaaaaat
     8521 cgcccgcgcc gccaccaaac gtagcggcac catcgctttt agcggcgagt atcacgggcg
     8581 cacgcattac acgctggcgc tgaccggcaa ggtgaatccg tactctgcgg gcatggggct
     8641 gatgccgggt catgtttatc gcgcgcttta tccttgcccg ctgcacggca taagcgagga
     8701 tgacgctatc gccagcatcc accggatctt caaaaatgat gccgcgccgg aagatatcgc
     8761 cgccatcatg attgagccgg ttcagggcga aggcggtttc tacgccgcgt cgccagcctt
     8821 tatgcagcgt ttacgcgctc tgtgtgacga gcacgggatc atgctgattg ccgatgaagt
     8881 gcagagcggc gcggggcgta ccggcacgct gtttgcgatg gagcagatgg gcgtggcgcc
     8941 ggatcttacc acctttgcga aatcgatcgc aggcggtttc ccgctggcgg gcgtcaccgg
     9001 gcgcgcggaa gtgatggacg ctgttgcacc gggcggtctg ggcggcacct atgccggtaa
     9061 cccgattgcc tgcgtggctg cgctggaagt gttgaaggtg ttcgagcagg aaaatctgct
     9121 gcaaaaagcc aacgatctgg ggcagaagct aaaagacgga ttgttggcga tagccgaaaa
     9181 acacccggag atcggcgacg tacgcgggct gggatcgatg attgccatcg agctgtttga
     9241 agatggcgat cacagcaaac cggacgcaaa actcaccgcc gagatcgtgg cacgcgcccg
     9301 cgataagggc ctgattcttc tctcctgcgg cccgtattac aacgtgctgc gcatccttgt
     9361 accactcacc attgaagacg ctcagatccg tcagggtctg gagattatcg gtcagtgttt
     9421 tgatgaggcg aagcagtagc accgtcccca tgcaggaggc gacgctgcgc gtcttgtccg
     9481 gcctacgaga atccaagtcg gataaggcgt ttacgccgca tccgacaatc tgtacgtgaa
     9541 caggaataaa tctatgtcgg ccggataagg cggagccgct ctccggcaaa aagaatcaat
     9601 aacaattaaa cgcgtgaccc gacgcgggaa atgtcggggc gctctcccaa gtgacacact
     9661 ttcgagagga ttcaggatgg ggcaatcatc gcaaccacac gagttaggcg gcgggctgaa
     9721 atcacgccac gtcaccatgt tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg
     9781 ttccagcgtc gccatcgccg aagcggaccc ggcggtatta ctggcctatc tgttcgccgg
     9841 attactggtg gttatgatta tgcggatgct ggcggaaatg gcagttgcca cgcccgatac
     9901 cggttcgttt tccacctatg ccgataaagc cattggccgc tgggcgggct ataccatcgg
     9961 ctggttgtac tggtggtttt gggtactggt tatcccactg gaagccaaca tcgccgccat
    10021 gatcctgcac tcgtgggttc caggcattcc catctggtta ttttccctcg tcattaccct
    10081 cgccttaact ggcagtaatt tattaagcgt taaaaactac ggcgaatttg agttctggct
    10141 ggcgctgtgc aaagtcatcg ctatcctggc ctttattttc cttggtgcag tcgcaattag
    10201 cggtttttac ccttatgccg aagtgagcgg gatctcaaga ttgtgggata gcggcggctt
    10261 tatgcccaac ggtttcggtg cggtattaag cgcgatgttg atcaccatgt tctcgtttat
    10321 gggcgcagaa attgtcacca ttgccgccgc ggaatccgac atgccggaaa aacatattgt
    10381 ccgcgccacc aactcggtta tctggcgtat ttctatcttc tatttgtgct ctatttttgt
    10441 cgtcgtggca ttaataccgt ggaatatgcc ggggctgaaa gccgttggat cttatcgctc
    10501 ggttctggaa ttgctcaata ttccccatgc gaaattaatc atggactgtg tgatattact
    10561 ttccgtaacc agttgcctga actcggccct gtataccgcc tcaaggatgc tctactcctt
    10621 aagccgtcgc ggtgatgctc ccgcggtaat gggtaaaatc aaccgcagta aaaccccgta
    10681 cgtggcggtg ttactctcca ccggcgcagc attcttaacg gtggtggtga actattacgc
    10741 gcctgcgaag gtatttaaat ttctgatcga cagctccggc gctatcgccc tgctggttta
    10801 tttagtcatc gccgtttcac agttgcggat gcgcaaaatt ctgcgagcag aaggaagcga
    10861 aattcgcttg cgaatgtggc ttgatccctg gctcacctgg ctggtcatcg gctttattac
    10921 ctttgtgttg gtagtgatgc tattccgtcc ggcgcaacag ttagaagtga tctccaccgg
    10981 cttattagcg atagggatta tctgtaccgt gccaattatg gctcgctgga aaaagctggt
    11041 attgtggcaa aaaacacccg ttcataatac gcgctgataa tgatcaggag tcacaccatg
    11101 accattacgt ctctggatgg ctatcgctgg ctgaagaacg atattattcg cggtaatttt
    11161 caaccggatg aaaaattacg gatgagttta ctgacatcgc gttatgcact tggcgttggg
    11221 ccgttgcagg aagcactttc tcaactggtg gcggaacggc tggtcacggt ggtgaatcaa
    11281 aaagggtatc gggtggcgtc tatgtcagag caggagctgc tcgatatttt cgacgcccgc
    11341 gccaatatgg aagcgatgct ggtcagtctg gcgattgccc gcggtggcga tgaatgggag
    11401 gcagacgttc tcgcaaaagc gcatctgctc agtaagctgg aagcctgtga cgccagcgag
    11461 aaaatgcttg atgagtggga tctgcgtcat caggcgtttc atacagcaat tgtggcgggc
    11521 tgtggttctc actatttgct gcaaatgcgt gaacggttgt ttgatctggc ggcgcgttat
    11581 cgatttatct ggctgcggcg aacggtgctt tcggtggaaa tgctggagga taaacacgat
    11641 cagcaccaga ccctgactgc ggcggtactg ccgcgagata cagcgcgcgc cagtgagtta
    11701 atgcgccagc atttactgac gccaattccc attatccagc aggcgatggc tggcaattaa
    11761 ctactcttcc ggaatacgca acacttgccc cggataaatt ttatccgggc tttttagcat
    11821 cggtttattc gcttcgaaga ttttattgta cagattagcg ttaccgtaga cctgtttgga
    11881 aatggcactc agagtgtcgc cagacttaac ggtataaaac tggctggcag tggctggtgt
    11941 cgccgttttc acctgatcat caacactggc aataccggaa atattcccca ccgcaacaag
    12001 gattttctcc ttcgcctcct gactcaggcc gtcaccagtg accgtcgctt tgccgtcggc
    12061 aatttgaatg ttcactttat cggcatcagg tataccggtt ttgctcagat gctcctgcac
    12121 cttcttcgcc tgatcgtctt tatcgtgctg acctgtaacc gcatcccaga gtttttctcc
    12181 ggcatcttta acaaaattga acagacccat agctacctca ttcgttaacg gaaacactca
    12241 gaaagtgtag cagaggaacg cggcgcgtta acgccgcggg ggtgagcaca ggtttaatcg
    12301 cgggtttgca cccagaacgc gtgaatcaaa ccggggatat acccaagcag tgtcagcaga
    12361 atattaataa tgaacgccca accgaaccct ttaccgagca gcacgccgat cggcggcaga
    12421 atgatggtga tgacgattct ccagaaaccc atatgtactc cctataagaa aattactcat
    12481 tgtttaaaaa gagattttat ctcttaagcg tagtaatttt agcggagcct gccagt
//
LOCUS       DAJTSF010000108        12408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000108 DAJTSF010000000
VERSION     DAJTSF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.108"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            356..1996
                     /gene="trg"
                     /locus_tag="OO335_003281"
     CDS             356..1996
                     /gene="trg"
                     /locus_tag="OO335_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR6003542.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2034..2957)
                     /locus_tag="OO335_003282"
     CDS             complement(2034..2957)
                     /locus_tag="OO335_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6003543.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3174..4517
                     /locus_tag="OO335_003283"
     CDS             3174..4517
                     /locus_tag="OO335_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6003544.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4742..6397
                     /gene="opgD"
                     /locus_tag="OO335_003284"
                     /gene_synonym="mdoD"
     CDS             4742..6397
                     /gene="opgD"
                     /locus_tag="OO335_003284"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6003545.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6537..6761
                     /locus_tag="OO335_003285"
     CDS             6537..6761
                     /locus_tag="OO335_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6003546.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6824..7360
                     /gene="rimL"
                     /locus_tag="OO335_003286"
     CDS             6824..7360
                     /gene="rimL"
                     /locus_tag="OO335_003286"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6003547.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7355..8335)
                     /locus_tag="OO335_003287"
     CDS             complement(7355..8335)
                     /locus_tag="OO335_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003548.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8459..9451
                     /gene="tehA"
                     /locus_tag="OO335_003288"
     CDS             8459..9451
                     /gene="tehA"
                     /locus_tag="OO335_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6003549.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9448..10041
                     /gene="tehB"
                     /locus_tag="OO335_003289"
     CDS             9448..10041
                     /gene="tehB"
                     /locus_tag="OO335_003289"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6003550.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10344..11000
                     /locus_tag="OO335_003290"
     CDS             10344..11000
                     /locus_tag="OO335_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR6003551.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(11018..11524)
                     /gene="tnpA"
                     /locus_tag="OO335_003291"
                     /pseudo
     CDS             complement(11018..11524)
                     /gene="tnpA"
                     /locus_tag="OO335_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11532..12408
                     /locus_tag="OO335_003292"
                     /pseudo
     CDS             11532..12408
                     /locus_tag="OO335_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3236 a         2908 c         3115 g         3149 t
ORIGIN      
        1 atcgtccaac aaaatgggtg cagtacagtt gatagaaata tcggtcgggg ccttcgtctt
       61 tctgattccc ggttagcctg aaaacagaaa gtctcaggca cccgcaggca tcctatgagg
      121 tttccttagg gacgaaaata atcacttcac gaaattgcgt gctgttttcc agaatttttc
      181 gtcattcggg ttagccagtt tagccattcg ttactctctt cattccaata gcattaattt
      241 tctctgcaat aattgttgta aaaatgtgac gcaaagaggt ttttggtcat aagtaattac
      301 cgtcaagtgc cgatgacttt ctatcaggag taaacctgga cgagagacaa cggtaatgaa
      361 tacaactccc tcacagcgat taggtttttt gcatcacatc aggctggttc cgttatttgc
      421 ctgcattctt ggcggtatct tagttctatt cgcattaagt tctgctctgg ctggctattt
      481 cctctggcag gccgatcgcg atcagcgtga tgttactgcg gagattgaga ttcggaccgg
      541 gttagcgaac agttcagatt ttttgcgttc agcccggatc aatatgattc aggccggggc
      601 tgcgagtcgt attgcggaaa tggaagcaat gaagcgaaat attgcgcaag ccgaatcgga
      661 gattaaacag tcgcagcaag gttatcgtgc ttatcagaat cgaccggtga aaacacctgc
      721 tgatgaagcc ttcgacactg aattaaatca acgctttcag gcttatatca cgggtatgca
      781 acctatgttg aaatatgcca aaaatggcat gtttgaagcg attatcaatc atgaaagtga
      841 gcagatccga ccgctggata atgcttatac cgatattttg aacaaagccg ttaagatacg
      901 tagcaccaga gccaaccaac tggcggaact ggcccatcag cgcacccgcc tgggtgggat
      961 gttcatgatt ggcgcgtttg tgcttgccct ggtcatgacg ctgataacat ttatggtgct
     1021 acgtcggatc gtcattcgtc cactgcaaca tgccgcacaa cggattgaaa aaatcgccag
     1081 tggcgatctg acgatgaatg atgaaccggc gggtcgtaat gaaatcggtc gcttaagtcg
     1141 tcatttacag caaatgcagc attcactggg gatgacagta gggactgttc gacagggcgc
     1201 ggaagagatt tatcgtggca ccagcgaaat ttcagctggc aatgcggacc tgtcatctcg
     1261 caccgaagaa caagcggcgg ctatcgaaca aactgccgcc agcatggagc aactcactgc
     1321 gacggtgaaa cagaatgcgg ataacgcgca tcatgccagc aaactggcgc aagaggcttc
     1381 tattaaagcc agcgatggcg ggcagacggt ttccggtgta gtaaaaacga tgggcgctat
     1441 ctccacgagt tcgaagaaaa tttctgagat caccgccgtc atcaacagta ttgctttcca
     1501 gacgaatatt ctggcactga atgctgccgt tgaagccgcg cgagcgggtg agcaagggcg
     1561 tggatttgcc gttgtcgcca gcgaagtacg gacactcgca agccgcagcg ctcaggcagc
     1621 gaaagagatt gaaggcttga tcagtgaatc agtcaggtta attgacctgg ggtcggatga
     1681 ggtggcaacg gcagggaaaa ccatgagcac tattgttgat gccgtcgcga gtgtcacaca
     1741 tatcatgcag gaaatcgccg ccgcctcgga tgaacaaagt agaggcataa ctcaggttag
     1801 ccaggcgatt tctgaaatgg ataaggtgac gcaacagaat gcttctctgg tagaagaggc
     1861 ctcagcagcg gcggtgtccc ttgaagaaca ggctgcacga ttaactgagg cggtgaacgt
     1921 attccgtctg aacaaacatt ctgtgtcggc agaacctcgc ggagcgggtg aaccagttag
     1981 tttcgctacg gtgtgaaaat gttcaaggag ggatcgacag atcccttcac ctttcagaac
     2041 ggcattgatt ttcgaatagc gttaatcatc aactggcaac cagaagagaa cgtcgcatct
     2101 acgcgggtca gtattccaat cggttcgcct gcaccatgtc ccggaacagg cagggccacc
     2161 agcgtggcat gacgcaggtc gtcttttaca gcgccagaag ggacaaacca cacgtaatcg
     2221 tattcaacgg taagttgacg agatagcgaa gtagacagcg tttcgataca acccgaagga
     2281 attttacagc cctggctctg cactaatgca tctgaatgct ggcgtggcgc agtgccttct
     2341 ggtgatacaa cgaccggcca ttccagcacc cggcttagcg ttacgttctc ctgaagtagc
     2401 gggtgattag ggcggacaac cagcttcaac gattcaagaa acagcagttc gtaattaagc
     2461 ccggtcatca gttcaggatc tgacatccga ccaatgccga tatcgatttc cccggttttt
     2521 aaacccgcca gaatcatagg gttactcatt gtcgcaactt gcaaggtcgt ctctttttgt
     2581 tgctgatgaa actgacctat aaccgaaggt aatatcccca gtgccgcagt aggtagtgca
     2641 ccaaccctga cgacatcatt attaagacct tctttacgat gaagcgactg tccggcagtg
     2701 ttgatggcgt caagaactct gactgcatgc gttaaaaatt gttcgccggg taaggtaagt
     2761 tgcgccccct gacgaccacg ctcaaacaag cgagcgcccg tcagctgctc cagttcattc
     2821 aatgtattag agagcgcagg ttgactcaaa ttaagggttt cagctgcgcg ccccaaagtt
     2881 ccttgttgtg cgacagctac gaatgtatga aggtggcgca aacggatgcg ctgactaaac
     2941 agactatttt tttccataag cgatgttaaa aacgaagcgg tgtcgctgac aagtgaagtt
     3001 gtttgattat gataacttga ttgcaaaata ttattaacaa ttaaagcaat tatgttacag
     3061 caaaatggat aatattgatg ttttcgcggc gagatcacag tttgtaaatt cttcccgcca
     3121 gagtgaatgc ggttacctac actccagatt actgaccact ggaggcagac actatggcga
     3181 acagcatcac ggcggatgag attcgggaac agttttcgca ggcaatgtca gccatgtacc
     3241 agcaagaagt tccgcaatat ggcacgctgc tggaactggt agctgatgtg aatctggctg
     3301 tgctggaaaa caatcctcaa ctgcacgaaa aaatggtaaa tgcagacgag ctggcgcgac
     3361 tgaatgttga acgtcatggg gcgattcgcg ttgggactgc acaagagctt gctactcttc
     3421 ggcggatgtt tgccattatg gggatgtacc cggtgagcta ttacgatctc tcgcaggcag
     3481 gggtgccggt acattcgaca gcatttcggc ccattgatga tgcttctctg gcgcgtaatc
     3541 ccttccgcgt ttttacatcc ttactccgcc ttgagcttat cgagaacgaa attttgcgcc
     3601 agaaagcggc ggagattctg cgtcagcgcg atatcttcac cccacgttgt cgacaactgt
     3661 tagaggaata tgagcagcag ggcggtttta acgaaacaca ggcacaggag tttgtgcagg
     3721 aagccctgga aacgtttcgc tggcaccagt tagcaacggt agatgaagaa acctatcgcg
     3781 ccttgcacaa cgaacatcgg ttgattgctg atgtggtctg ttttcctgga tgccatatca
     3841 accacctgac gccacgtacg ctggatattg accgggtgca gtcgatgatg cctgaatgcg
     3901 gaattgaacc caaaattctc atcgaggggc cgccgcgccg cgaggtaccg attttactac
     3961 gccagaccag ctttaaagca ctggaagaga cggtgttgtt tgcggggcag aaacagggca
     4021 cgcataccgc gcgctttggt gaaattgagc agcgtggcgt ggcattaacg ccgaaagggc
     4081 gacaactgta tgatgatctt ctgcgtaacg ctggaaccgg gcaggataat ctcactcacc
     4141 aaatgcattt acaggaaacc ttccgcactt ttcctgacag tgagttttta atgcgtcagc
     4201 aagggttggc atggttccgg taccgtctga cgccttcggg tgaggcgcat cgtcaggcga
     4261 ttcatcctgg agacgatcca cagcccttaa ttgaacgtgg ttgggtagtg gcgcaaccca
     4321 tcacctatga agatttcttg cccgttagcg cggcggggat cttccagtca aatctgggta
     4381 atgaaacgca gacacgcagt cacggtaatg ccagtcgcga agcatttgag caggcgttgg
     4441 gttgtccggt tttggatgag ttccagcttt accaggaagc ggaagaacgc agtaaacgtc
     4501 gctgtggttt gctttaaatt ctgaccatcc gcctttgcaa aaatttgcct gattttacaa
     4561 acgaatcagg ctcatcccat cgacataaaa aaaatgccga tttatgcata ttctctcagt
     4621 tcaacaattg gattattaat aaatattgtc tagagtgagc ggtcataaat aagcactttc
     4681 ttgccgctga aaacgaccag cgcgggacca ttcacaacac cagaaggact cactttcagg
     4741 tatggatcgt agacgattta ttaaaggttc aatggctatg gccgccgtgt gcggtaccag
     4801 cggcattgct tctctttttt ctcaggcagc attcgcggca gattctgata ttgccgacgg
     4861 gcaaacccag cgttttgact tctccattct acagtcaatg gcgcacgact tagcgcaaac
     4921 agcgtggcgt ggtgcgcctc gtccgttacc tgacacgctg gcgacaatga cgccgcaggc
     4981 ttataacagt attcaatacg acgccgaaaa atcgctctgg cataacgttg agaaccgtca
     5041 actggacgct cagttcttcc atatgggaat gggattccgt cgccgcgttc gtatgttttc
     5101 tgtagatcca gcaacacatc tggcgcgtga aattcacttt cgcccggagt tgttcaaata
     5161 caacgatgca ggtgttgata ccaaacaatt agaagggcaa agcgatctcg gctttgccgg
     5221 ttttcgcgtg tttaaagccc ccgaactggc gcgccgtgat gtagtatcat ttctcggcgc
     5281 gagttatttc cgcgccgttg atgatacata tcaatacggt ttgtcggccc gcggcctggc
     5341 gatcgacact tacaccgaca gtaaagaaga gttccccgac tttaccgcct tctggtttga
     5401 tacggtaaaa ccgggggcaa ctacctttac cgtttatgcg ttgctcgata gcgccagcat
     5461 tactggtgcc tataagttca ctatccattg tgagaaaagt caggtgatta tggatgtgga
     5521 aaatcacctg tatgcgcgca aagacattaa acagctgggc atttcgccga tgaccagtat
     5581 gttcagctgc ggtactaatg aacgtcggat gtgcgataca attcatccgc aaattcatga
     5641 ctctgatcgt ctgtccatgt ggcggggcaa cggcgagtgg atttgccgtc cgctgaataa
     5701 tccgcaaaaa ttgcagttca atgcttacac cgacaacaac ccgaaagggt ttggtttatt
     5761 gcaactggat cgtgacttct cccattatca ggacattatg ggctggtata acaaacgccc
     5821 aagtctgtgg gtggaaccgc gtaacaagtg gggtaagggc accatcggcc tgatggaaat
     5881 cccaacaacg ggcgaaacgc tggataacat tgtctgcttc tggcagccag aaaaagctgt
     5941 aaaagcaggt gatgagtttg cattccagta tcgtctgtac tggagtgcgc aaccgcctgt
     6001 tcattgccca ttagcgcgcg ttatggcgac gcgtaccggc atgggcggtt tcccggaagg
     6061 ttgggcgcca ggtgaacact atcccgaaaa atgggcgcgt cgttttgccg tcgatttcgt
     6121 tggtggtgat ctgaaagctg ccgcgccaaa aggcattgag ccggtgatta cgctttccag
     6181 tggggaagcg aagcaaatcg aaattctcta tattgaaccc atcgatggtt atcgtattca
     6241 gtttgactgg tatccgactt cggactccac tgatccggtc gatatgcgga tgtatctacg
     6301 ttgtcagggg gacgctatca gtgaaacatg gctgtatcag tatttcccgc cagcgccgga
     6361 taaacgtcag tatgttgacg accgcgtgat gagttaatcg ttttttcttc ggcaccttct
     6421 tcgggaggtg ccgtctggtt aaacacgatc ccgctcgcat ttttccctaa gttaaatgag
     6481 taatctgatg gtgtgtattt cagatacacc gtgtcagcca ctaacaggga gtgcgtatgt
     6541 ttccagaata ccgagattta atatcccgtc tgaaaaacga aaatcctcgc tttatgtcct
     6601 tgttcgataa acacaataaa cttgatcatg aaattgccag aaaggaaggt tccgacggtc
     6661 gagggtacaa tgcggaagtg gtccgcatga aaaaacaaaa gctacagtta aaagatgaga
     6721 tgctcaaaat cctgcagcag gagagcgtca aagaggtgta aactttccta agccgccagc
     6781 caggcggctt ttttaacaac agcatggatt gactggagat aagatgactg aaacgataaa
     6841 agtaagcgaa tcacttgaat tacatgctgt tgcggaaagt cacgtcacac ctctttatca
     6901 gttaatctgt aaaaataaaa cctggttaca gcagtcgcta aactggccgc agttcgttca
     6961 gagtgaagag gacacgcgaa aaacggtgca gggtaatgtg atgttgcatc aacgcggcta
     7021 tgccaaaatg ttcatgattt tcaaagaaga tgaacttatc ggcgttatct cgtttaatcg
     7081 tattgaacca ctgaataaaa ctgctgaaat cggctactgg ctggacgaat ctcatcaggg
     7141 gcaggggatc atttctcagg cgctgcagac attgattcat cattacgctc agtctggtga
     7201 acttagacgc ttcgtgatca aatgtcgggt ggacaatccg caaagcaacc aggtcgcttt
     7261 gcgcaatggt tttatccttg aaggttgcct gaaacaggct gagttcctga atgatgccta
     7321 tgatgatgtg aacttatacg cgcgtattat cgattcataa taaccctgcc agcggcgttc
     7381 gcgtaatgcg ctcctcgccg ttaatgactt ttggaccacg gacatgaacc gtgtcgccat
     7441 caaaggcttc aacgacggcg tggtcggtta actccacatg gttttcaatt atcaccgcac
     7501 ctgtaatacg gctctcacct tgtattacaa cgtgctcgtc gagcagaatc ggtccaccac
     7561 gtaccacggc atttccgcca atcaagacat gatgttttaa cacacaatta ccttccacaa
     7621 tggcatattc cgccacctgc gaactgtaat gaatcgtcgg aatggcatct tcttctatgc
     7681 cagctttcac ctgcgcgtga ccgtagactt tagcgcaatc gcatagccag acgttgttct
     7741 cttcattacc ttcaacactg gcaaaatcaa aaacttcggc gcgatgttca ataaaagcat
     7801 accggacgac ggcatcgcca taaatttgtg cctggtggac aatacgcgag gcgctaaccc
     7861 ttgcgcgatc ataaatttgg agcaggagtt gatggtcggg cgttaagcct tgtgcggcga
     7921 cgatcataga atgttgatca attaaggcgt gaccaaatat tctacattgc acgtaaacta
     7981 atgagtcatg aatggtgaca ctgtcactta tatatgcacc ctggctgatt tcactattat
     8041 cgatccagac gttatccgtt gcgtaaacct ctccccacag cacgctggtg ccggtgatac
     8101 gcgtattacc ggaaatcacc gttccgccga acgcaatagc attctggtcg taaatccagc
     8161 aatttccttc ctgtgctaac accgtttcgc gatcgatcca gccgccagcg gttcctgcta
     8221 tcacatcgtt aaaatcgcta atggcgataa tctgccgtaa taacacactt tttttagtgc
     8281 catcttcctg ataactaaag gctcgctgtt cttcacttag gcgatatttg cgcatagcgt
     8341 tttcccacag gtgacttact ataaccgtag caaattctgc ggctctggct atgctcgaga
     8401 aaattccata aaatgcattt caaatatact ttataaatta aacaaaatga gtaagaagat
     8461 gcagagcgat aaagtgctca atttgccggc aggctacttt ggtattgtgt tggggacgat
     8521 agggatggga tttgcctggc gctatgccag ccaggtttgg caggtcagcc actggttagg
     8581 ggatgggctg gtgattctgg cgatgatcat ctggggatta ttgactagcg catttattac
     8641 ccgactcata cgctttccgc atagcgtgct ggcggaagtt cgccatccag tgctgagcag
     8701 ttttgtgagt ttgtttccgg caacgacgat gctggtggcg attggttttg ttccgtggtt
     8761 tcgcccactg gcggtgtgca tgttcagttt tggtgtcgtg gttcagttgg cttatgccgc
     8821 ctggcaaact gcgggattat ggcgcggatc tcaccctgaa gaagctacca cgcctggact
     8881 gtatctgccg acagttgcca acaactttat cagcgcaatg gcctgtggtg cgttgggcta
     8941 caccgacgcc ggtctggtgt ttttaggcgc aggcgttttc tcatggctaa gccttgaacc
     9001 ggtgatcttg cagcgtctgc gcagttcggg agaattaccc acggcactgc ggacatcact
     9061 cggcattcag ctcgctcctg cgctggtggc ctgtagtgcc tggctgagcg tcaacggcgg
     9121 cgagggtgac acgctggcga aaatgctttt cggttatgga ctgctgcaac tgctgtttat
     9181 gctacgtctg atgccatggt atctctccca gccatttaat gcttcattct ggagtttctc
     9241 gttcggcgta tctgcactgg caaccaccgg tttgcatctg gggagtggca gcgataatgg
     9301 atttttccat acgctggcgg tgccgctgtt tatctttacc aattttatta ttgcaatact
     9361 gctcatccgt acttttgcgc ttctgatgca gggaaaattg ttagtcagaa ccgagcgcgc
     9421 cgttttaatg aaagcagagg acaaagaatg atcattcgtg acgaaaacta ttttactgat
     9481 aaatatgaat taacccgcac acactctgaa gtactggaag cggtgaaagt ggttaaaccg
     9541 ggtaaaacgc tggatctggg ctgtggcaat ggtcgtaaca gtctttacct ggcagccaat
     9601 ggttatgatg ttgacgcatg ggataaaaat gccatgagta tcgccaacgt cgagcgcatt
     9661 aaatccattg aaaatctgga taatttacac acccgggtcg ttgatctgaa taacctcaca
     9721 tttgatggac agtacgattt tattctttcg actgtggtgc tgatgttcct tgaggctaaa
     9781 accatcccag ggttgattgc caatatgcaa cgttgcacta aacctggtgg ttacaacctg
     9841 attgtggcgg cgatggatac cgctgattat ccatgtaccg tcggcttcac gtttgccttc
     9901 aaagagggag aattacgtcg atattacgaa ggctgggaga gggtgaaata caatgaagac
     9961 gtcggcgagc tgcaccgcac cgacgccaac ggtaatcgta ttaaactgcg tttcgccacg
    10021 atgctggcac gtaaaaaatg acccggtaag cacaaaacgc gtgaaaattc cccacgctga
    10081 gatgatttac tgttcttctt ttcggtaagc atatttttta tcgaagggat gtgaaattaa
    10141 tcacagtagt cgaagttttt tagcagctta acttactgaa atttaactac tgatgattga
    10201 cttagcccct ttttcggcat tgactatgtc gtctgaaaag gggctgaaaa atttattttc
    10261 accaacactt tttttgccac aacacgaagc ggcgcttttt gctataactt agaaagtaat
    10321 ataatcatct caggaaacta ttcatgcgta ccacatcatt tgcgaaagtt gcagctttat
    10381 gcggcttgtt ggctctgtct ggttgtgcct ctaaaatcac ccagccagat aaatataaca
    10441 attactctga tttaaaagaa acaacctcag caacaggtaa acctgtttta cgttgggtag
    10501 acccgagttt tgatcaaagc aaatatgaca gcatcgtctg gaacccaatc acttattatc
    10561 cggtaccaaa accttctacc caggtagggc agaaagttct ggataaaatt ttgaactata
    10621 ccaacaccga aatgaaagaa gcgatagcgc agcgtaaacc agtggttacc accgctgggc
    10681 cgcgtagtct gattttccgt ggggccatta ccggtgtaga taccagcaaa gaagggctgc
    10741 aattctatga agtggttcct gttgcattag tggttgcggg gacgcaaatg gctacaggcc
    10801 accgtaccat ggatactcgc ctctattttg aaggtgagct gattgatgca gcgactaata
    10861 aaccggttat caaagtcgtt cgtcagggcg aaggtaaaga cctgaataac gaaagtacgc
    10921 caatggcttt cgaaaatatt aaacaagtta ttgatgacat ggcgaccgat gccaccatgt
    10981 ttgacgttaa caaaaagtag ttccagacgc gccatcgtta gatggcgctt tttatccggt
    11041 gcgccgtaaa accccatcct tcagggcggg gatataaggc gcggttttcc acctgaccag
    11101 gtgtttgctg ttgttcaatg tattggcgga tgacggatat tggcgcacct ccgcagctac
    11161 tggcaaaata gccaggactc cacaaaacgc ctttgtagta atacctgact gcaatatctg
    11221 gtcgatctcg tcgcagtaac ctacccgata cgcctttgag gctgtttacc agactggata
    11281 tcgccagttt gggaggttag ttaattaaca aatggacgtg atctggttcg ccatccattt
    11341 caaccagttc agcttcaaaa taagcacata catttgaaaa gtaagtgcgt agtttttctg
    11401 tcgcgtcatg gtcaaaaatc tggcgtcggt atctggtgac aaagactagg tgaacatgca
    11461 tcaggaaaac acaatgcctt ccacgccgga tatcggtttc ttttttcaca gaccaaagta
    11521 tcatttgacc tgtgaaacga ttacaggcat ttaaattcca gttaagacca ggtggtcaac
    11581 aggagtgtga aatgaggcgc ttcgcaggcg catgtcgttt cgttttcaat cgtgctctgg
    11641 cacgtcagaa tgagaatcat aaggccggta ataaatacat cccttacggg aaaatggctt
    11701 cctggctggt tgagtggaaa aatgccactg aaacgcaatg gcttaaagat tctccctcac
    11761 agccattgca acagtcactg aaagaccttg agcgggctta caaaaacttc ttccggaagc
    11821 gggctgcttt tccccgattc aaaaagcggt gacagaatga tgcattccgc tacccgcagg
    11881 gcgttaagct cgatcaggaa aacagccgta tttttctgcc gaaactgggc tggatgcgct
    11941 accggaatag ccgtcaggtc acgggtgttg tgaaaaatgt cactgtcagc cagtcctgcg
    12001 gtacgtggta catcagtatt cagacagaaa gtgaagtatc cactccggct cacccttcag
    12061 catcaatggt cgggctggat gctggcgtgg ctaaactcgc cacgctgtca gatggcacag
    12121 tctttgagcc tgtaaacagt tttcagaaaa accagaagac gctggcgaga cttcagcgac
    12181 agttaagccg caaggtcaaa ttcagcaaca actggcagaa gcagaaacgc aaaatacagc
    12241 gactgcattc ctgtatcgca aatatccgca gggactacct tcacaaagtc acaacggccg
    12301 tcagcaaaaa ccacgcaatg atagtcattg aggatttgaa ggtcagcaac atgtcaaagt
    12361 cagcagcggg tacggtcagc cagccggggc gcaatgtccg ggcaaaat
//
LOCUS       DAJTSF010000109        12298 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000109 DAJTSF010000000
VERSION     DAJTSF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.109"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO335_003293"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO335_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR6003552.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="OO335_003294"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="OO335_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR6003553.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="OO335_003295"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="OO335_003295"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR6003554.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="OO335_003296"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="OO335_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6003555.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="OO335_003297"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="OO335_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR6003556.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="OO335_003298"
     CDS             4025..4615
                     /locus_tag="OO335_003298"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR6003557.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="OO335_003299"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="OO335_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR6003558.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="OO335_003300"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="OO335_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR6003559.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="OO335_003301"
     CDS             complement(6551..6658)
                     /locus_tag="OO335_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003560.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="OO335_003302"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="OO335_003302"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6003561.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="OO335_003303"
     CDS             7648..7875
                     /locus_tag="OO335_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR6003562.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO335_003304"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO335_003304"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR6003563.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="OO335_003305"
     CDS             complement(9447..10703)
                     /locus_tag="OO335_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR6003564.1"
                     /translation="MGSNIHGISCTANDYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="OO335_003306"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="OO335_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6003565.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="OO335_003307"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="OO335_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6003566.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
BASE COUNT         2934 a         3123 c         3109 g         3132 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa tatccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc attatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 gactctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aatcatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg tggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggt
//
LOCUS       DAJTSF010000110        12221 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000110 DAJTSF010000000
VERSION     DAJTSF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.110"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..492)
                     /locus_tag="OO335_003308"
     CDS             complement(<1..492)
                     /locus_tag="OO335_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6003567.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(523..1215)
                     /gene="fimC"
                     /locus_tag="OO335_003309"
     CDS             complement(523..1215)
                     /gene="fimC"
                     /locus_tag="OO335_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6003568.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="OO335_003310"
     CDS             complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="OO335_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6003569.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2458..3324
                     /gene="folD"
                     /locus_tag="OO335_003311"
     CDS             2458..3324
                     /gene="folD"
                     /locus_tag="OO335_003311"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6003570.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3326..3538
                     /gene="ybcJ"
                     /locus_tag="OO335_003312"
     CDS             3326..3538
                     /gene="ybcJ"
                     /locus_tag="OO335_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6003571.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3646..4155
                     /locus_tag="OO335_003313"
     CDS             3646..4155
                     /locus_tag="OO335_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6003572.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="OO335_003314"
     CDS             complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="OO335_003314"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6003573.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5750..6244
                     /gene="ppiB"
                     /locus_tag="OO335_003315"
     CDS             5750..6244
                     /gene="ppiB"
                     /locus_tag="OO335_003315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6003574.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6247..6969
                     /gene="lpxH"
                     /locus_tag="OO335_003316"
     CDS             6247..6969
                     /gene="lpxH"
                     /locus_tag="OO335_003316"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6003575.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7087..7596
                     /gene="purE"
                     /locus_tag="OO335_003317"
     CDS             7087..7596
                     /gene="purE"
                     /locus_tag="OO335_003317"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6003576.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7593..8660
                     /gene="purK"
                     /locus_tag="OO335_003318"
     CDS             7593..8660
                     /gene="purK"
                     /locus_tag="OO335_003318"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6003577.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8797..9690)
                     /locus_tag="OO335_003319"
     CDS             complement(8797..9690)
                     /locus_tag="OO335_003319"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6003578.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9687..10334)
                     /locus_tag="OO335_003320"
                     /pseudo
     CDS             complement(9687..10334)
                     /locus_tag="OO335_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10337..11569
                     /gene="allC"
                     /locus_tag="OO335_003321"
     CDS             10337..11569
                     /gene="allC"
                     /locus_tag="OO335_003321"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR6003579.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11580..>12221
                     /gene="allE"
                     /locus_tag="OO335_003322"
     CDS             11580..>12221
                     /gene="allE"
                     /locus_tag="OO335_003322"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR6003580.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYN"
BASE COUNT         2968 a         3159 c         3116 g         2978 t
ORIGIN      
        1 agcgctactc aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc
       61 aaagttaatt gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg
      121 ctgggctaac tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt
      181 aaaacagggc attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac
      241 gatatcctgt gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc
      301 aggttgatga ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa
      361 taaaaatgcc gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag
      421 agatgaataa caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt
      481 gggtattttc ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc
      541 tctgaccggg gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac
      601 tcctgagggt aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt
      661 ttggctgccc acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt
      721 gatatagtta ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg
      781 taattgaatt ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt
      841 cctgccgttc aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag
      901 agactcacga tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct
      961 tttgggttca ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct
     1021 gctgttttca atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca
     1081 taccgcagtc tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc
     1141 tccggcagca tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt
     1201 tattttagtc atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat
     1261 aaagaaatga ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg
     1321 catgatacaa agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg
     1381 gtacattact ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc
     1441 gtatctcata ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt
     1501 gcctttataa cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt
     1561 tgagaagcta ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc
     1621 gacacccgtt gctgttgttg tatttgtact ggatacaatg gccaataaat tatcattgat
     1681 tgcatcagcc tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc
     1741 gttcaactga atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc
     1801 ggtacgatat tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc
     1861 agcattcacc agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact
     1921 acccgtagcg gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata
     1981 tattccttca atctcattta tcaactccac atccgtatat aaccgattac tttatttaag
     2041 acactgatag tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca
     2101 ctctattcta aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcac
     2161 cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg
     2221 tatttcttaa attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg
     2281 ccatcatgta cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac
     2341 cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct
     2401 tctgacaaaa tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg
     2461 gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa
     2521 aaagttcagg ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg
     2581 ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc
     2641 gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag
     2701 cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta
     2761 ccggcgggta ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac
     2821 ggtttccatc cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc
     2881 accccgcgcg gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac
     2941 gccgtggtga ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc
     3001 gcaggttgca ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa
     3061 aatgccgatc tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc
     3121 aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg
     3181 ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc
     3241 ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa
     3301 tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt
     3361 tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat
     3421 cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa
     3481 aatcgtcgcc ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc
     3541 cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc
     3601 gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt
     3661 acgcacgccg ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt
     3721 gccggaatca tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt
     3781 gcttacggca atgtttttgg tcatcgcggt tttacccatt cgctggtgtt tgcgtttgtt
     3841 gtcccgctct tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc
     3901 tggctgtttt taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc
     3961 ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag
     4021 gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg
     4081 attatttccg aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg
     4141 tggcgcagac gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga
     4201 cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca
     4261 cgtgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt
     4321 aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct
     4381 tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt
     4441 gcattcgccg ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac
     4501 agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct
     4561 tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag
     4621 aggcgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg
     4681 cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca
     4741 tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc
     4801 attccggagt gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg
     4861 atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg
     4921 ttacccagct gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg
     4981 ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc
     5041 atccggttgc gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga
     5101 tcctgacgcg acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgctg
     5161 ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct
     5221 gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa
     5281 tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca
     5341 ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta
     5401 tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga
     5461 ccgatatgac agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg
     5521 tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa
     5581 gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg
     5641 aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat
     5701 atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt
     5761 ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt
     5821 taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt
     5881 tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac
     5941 caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc
     6001 aatggcacgt actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga
     6061 taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc
     6121 tgaagtggtt gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtaa
     6181 cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga
     6241 gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc
     6301 accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt
     6361 cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag
     6421 atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc
     6481 aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg
     6541 gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga ttatgcatgg cgacacgctg
     6601 tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa
     6661 atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg
     6721 aacagcaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct
     6781 gtggtcagtg cgatggaaaa acatcaggtg caatggctaa tccacgggca tacccatcgc
     6841 ccggcggtgc atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc
     6901 tggcatacgg aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt
     6961 ccgttttaaa aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc
     7021 tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta
     7081 agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc
     7141 gactgggcta ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac
     7201 gttgaagtgg tttctgctca ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc
     7261 gaagagaacg gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc
     7321 atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg
     7381 agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg
     7441 ctggcgattg gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg
     7501 actcacgata aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa
     7561 gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg
     7621 ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg
     7681 tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga
     7741 tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga
     7801 accgcgatgt gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc
     7861 tccacctgcc gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt
     7921 ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg
     7981 gtcaatggag tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacggtgaat
     8041 gtattgtcga gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct
     8101 ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc
     8161 gcaccagcgt cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc
     8221 tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca
     8281 ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga
     8341 cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc
     8401 cgttaccgca accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg
     8461 tgaattatga ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc
     8521 gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg
     8581 cgacgctgga agccttaatc ccgctgctgc cgccagagta tgccagcggc gtgatttggg
     8641 cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct
     8701 tatccggctt accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat
     8761 caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc
     8821 ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg
     8881 gcttctgaca taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc
     8941 aaatggcgct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca
     9001 gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc
     9061 ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc
     9121 cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa
     9181 ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa
     9241 atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc
     9301 tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga
     9361 aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct
     9421 ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata
     9481 cggttctacc tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt
     9541 gccgtgaaca atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact
     9601 ggcgatattg cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt
     9661 gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc
     9721 cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc
     9781 gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg
     9841 aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga
     9901 agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac
     9961 caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct
    10021 ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga
    10081 cgaaactggc gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa
    10141 agtccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc
    10201 ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga
    10261 atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg
    10321 aattgcgtgc ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct
    10381 ttcctctttt ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg
    10441 gctggaaacc cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt
    10501 cgatgaagtg gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt
    10561 tctgagcggt tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg
    10621 cgcgctggcg gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg
    10681 tacggtcgaa gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg
    10741 gggcagtaaa aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc
    10801 caaaggaaat agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc
    10861 actaactccg cgtcaggcta taaaagcctt tgttgaactg catattgaac agggctgtgt
    10921 gctggaaagt aatgggcaat caattggcgt ggtgaatgca attgtcgggc agcgtcgcta
    10981 tacggtgacg ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg
    11041 tgatacggtt tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat
    11101 gggcgatccg ctggttctga cctttggcaa agtagagccg cgcccgaata cggtaaatgt
    11161 ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat accgacgctg ccgtgctgcg
    11221 cgatttcacc caacagttag aaaacgacat gcgggcgatt tgcgatgaaa tggacattgg
    11281 tattgatatc gatttatgga tggacgaaga acccgtgccg atgaataagg agctggtcgc
    11341 caccctgaca gaattgtgtg aaagagaaaa actgaattac cgggtgatgc acagtggtgc
    11401 cgggcacgac gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag
    11461 catcaacggg atcagccata acccggcgga acgcaccaat attactgacc ttgccgaagg
    11521 ggtcaaaacg ttggcactca tgctttatca acttgcctgg caggaataag gagtcataaa
    11581 tgggatattt aaataacgtc accggttacc gcgaagattt actggctaac cgtgcgattg
    11641 ttaaacacgg taatttcgca ctgttaaccc cggatggtct gataaaaaat attattccgg
    11701 actttgaaaa ttgtgacgcg acaatcctct ccacgccaaa gctgggtgcc tcttttgttg
    11761 attatctggt cacactgcat caaaacggtg gcaaccaaca gggcttcggt ggcgaaggca
    11821 ttgaaacgtt cctgtatgtg atctctggaa atatcactgc caaagccgaa ggcaaaacat
    11881 ttgccttaag cgaaggtggc tatctttatt gcccaccagg ctccttaatg acgtttgtta
    11941 acgcccaggc cgaagacagc caaatctttt tatataagcg ccgctatgtt ccggtagaag
    12001 gccatgcacc gtggctgttt tctggcaatg ccagcgaact ggaacgcatt cactatgaag
    12061 gtatggatga tgtgatcctg ctggattttc tgcccaaaga gttaggtttt gatatgaaca
    12121 tgcatatcct ctcttttgca ccaggtgcca gccacggtta tatcgaaaca cacgttcagg
    12181 aacatggtgc ctatattctt tccggtcagg gggtttataa c
//
LOCUS       DAJTSF010000111        12154 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000111 DAJTSF010000000
VERSION     DAJTSF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.111"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            126..995
                     /locus_tag="OO335_003323"
     CDS             126..995
                     /locus_tag="OO335_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR6003581.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="OO335_003324"
     CDS             complement(1198..1551)
                     /locus_tag="OO335_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6003582.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="OO335_003325"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="OO335_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6003583.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="OO335_003326"
     CDS             complement(3379..3672)
                     /locus_tag="OO335_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6003584.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="OO335_003327"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="OO335_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6003585.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="OO335_003328"
     CDS             complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="OO335_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6003586.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="OO335_003329"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="OO335_003329"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6003587.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="OO335_003330"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="OO335_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6003588.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="OO335_003331"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="OO335_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6003589.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="OO335_003332"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="OO335_003332"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6003590.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="OO335_003333"
     CDS             11052..11627
                     /locus_tag="OO335_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6003591.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="OO335_003334"
     tRNA            11734..11809
                     /locus_tag="OO335_003334"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2932 a         3140 c         2980 g         3102 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctag cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggccgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa caat
//
LOCUS       DAJTSF010000112        12112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000112 DAJTSF010000000
VERSION     DAJTSF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.112"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..370
                     /locus_tag="OO335_003335"
     CDS             <1..370
                     /locus_tag="OO335_003335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003592.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="OO335_003336"
     CDS             complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="OO335_003336"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR6003593.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="OO335_003337"
     CDS             complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="OO335_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR6003594.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="OO335_003338"
     CDS             complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="OO335_003338"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR6003595.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="OO335_003339"
     CDS             complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="OO335_003339"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR6003596.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3448..5049)
                     /gene="tap"
                     /locus_tag="OO335_003340"
     CDS             complement(3448..5049)
                     /gene="tap"
                     /locus_tag="OO335_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR6003597.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5095..6756)
                     /gene="tar"
                     /locus_tag="OO335_003341"
     CDS             complement(5095..6756)
                     /gene="tar"
                     /locus_tag="OO335_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR6003598.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="OO335_003342"
     CDS             complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="OO335_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR6003599.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="OO335_003343"
     CDS             complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="OO335_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR6003600.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9394..10320)
                     /gene="motB"
                     /locus_tag="OO335_003344"
     CDS             complement(9394..10320)
                     /gene="motB"
                     /locus_tag="OO335_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR6003601.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10317..11204)
                     /gene="motA"
                     /locus_tag="OO335_003345"
     CDS             complement(10317..11204)
                     /gene="motA"
                     /locus_tag="OO335_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR6003602.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="OO335_003346"
     CDS             complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="OO335_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR6003603.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11912..>12112)
                     /locus_tag="OO335_003347"
     CDS             complement(11912..>12112)
                     /locus_tag="OO335_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR6003604.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2782 a         3388 c         2934 g         3008 t
ORIGIN      
        1 cactacgcta atgagttggg atgccgtgct ctatgatcta cgtagtgaaa aaattatcag
       61 taaagataat tacttgaccg acttaaaccg cagacatctt gaacttgttc ttcatactaa
      121 ccccaatcca agcggttcgg taacgaagat tttacgcact atttttacta agcaggtaaa
      181 gagtttaggg ccggaactct gtcatttctt gcaccatgaa ctgacgaaaa aacgttatga
      241 agtatggcat cactacgatg tactgcacta ccagtcatcg tcattttctc gttcacaatt
      301 taatgcattg ataaatttac tggcgcaaca atcacgcttc gctgatctaa aattatcaaa
      361 ctacaaataa ccatcctcca cgtactccct gaacgatacc cgccaccctg ctatcggctt
      421 ggcatcatcg cgcaggccga agttcaggca atacaaatca aaatccaaga ctatccaaca
      481 aatcgtccac ctgatcctga ctggctacca cgccggcttt gctggtatcg acctgaggtc
      541 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
      601 acagcaccat cagcaactgg cgttcgatct cctgaatgac atccatcatc cgcttaataa
      661 cctgcccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
      721 taaagctggt atgcgcgggt acatctgcca gaaattgtcg tgtatctgtt accagttcac
      781 gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
      841 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
      901 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
      961 tggcttccgc cgcttcggca atggcctgaa ccagccccag ttcccgcaaa ctgtcgcgca
     1021 gcatatgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
     1081 gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc aaagattttg
     1141 ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc actggccccc
     1201 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
     1261 aatgccgaca tcgcgccatc cgcacgaatt gttttcagca attccaggcc atccatattg
     1321 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
     1381 tcgacaccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
     1441 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
     1501 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattgtt gccaacattt
     1561 gctggcttac ctggctaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
     1621 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gcccccgcct
     1681 gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg atcaccccaa
     1741 ccgcattacg ccccgcctgt ttggcgacag aatggaacaa cacatctacc gaaggccgat
     1801 gacggttaac cgccgggccg tcgtgaattt tgatttgata atttgcgcca ctacgcgcca
     1861 gttccatatg ccgatcgccc ggcgcaatat aggcatgccc cggcaagaca cgttctccgt
     1921 cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca aaagagcggg
     1981 tgaaaccggg cggcatatgt tgggtaatta acaatgccgg gctggaaagc ggcaacggtt
     2041 gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca atcagttttt
     2101 cagaactcaa caacggccct gccttcagcg ttgtcggtgc tgacaatggc ttatgtgctg
     2161 caaggctcgc ctttgctgcc gtacgcacct tttcagcaat catttcgcta tacgccagca
     2221 ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tattgccccc agctccagcg
     2281 cgcgcagcgt gacttctgat cctttgccgg tcaaggaaga aaccatcaca acgggcattg
     2341 gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc atttcaacat
     2401 ccagcgtcag cacatcggga ttgaatttct taatcaagtc acgcgcaacc agcggatcag
     2461 gcgcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc atgatctggc
     2521 gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt aatccttact
     2581 tagcgcatac accgtctgac cacgcagcgt gaagcggcgc tcgaggtggc taaagttttc
     2641 agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc ggcgcaaaat
     2701 ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga tcgcatcaaa
     2761 cggccccggc acggtgtact gtttcgccag taaattcagc ggggcaaaat caacatagtt
     2821 cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc gcatgaaata
     2881 ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataga taccgcttct
     2941 ggctttttcc agcacttcgg tgtcgatatc actggcaaac actttccagc gtccgggcac
     3001 ggtgcccaat gtgtcagcca gagtcatcgc aatgctgtac ggctcttcgc cggtcgaagc
     3061 cgccgcgctc catacgcgat cctcgccaga acggcgacgt gcgtgatccg cgagcagagg
     3121 gaaatgatga gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc
     3181 ctgccactca ccgctgtgct gattagattc cagcaagttc agataatgac cgaaatccgt
     3241 cagtcccaga gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc gtttatggtc
     3301 agccagaacg atcccggctc gttgatagat caattgactt atccgccgaa aatgcgcgtc
     3361 ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac agggcagaga
     3421 tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt gtaactgcgc
     3481 cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac gcgacgaaag
     3541 atggtcggcc tgattcgcca gttgttccgt tgccaccgcc gcttcttcta ccagcgagga
     3601 gttctgctga gtcacctgat ccatctggct gacagcctgt gcaacctgct caatcccccg
     3661 ccgttgttct tccgacgccg aagcaatttc tcccataatg tcgttcacgc gggtcaccga
     3721 actgacaata tcggtcatgg tcgcggcggc gttattcacc agtttcgaac cctgctggac
     3781 acgattgact gactcttcga tcagcccttt gatctctttt gctgcctgcg cgctacggct
     3841 ggcaagattg cgcacttcac ctgccactac cgcaaaacca cgcccctgct ctccggcgcg
     3901 agccgcttcc actgccgcat tcagggccag aatattggtc tggaaagcaa ttccgtcgat
     3961 aacgctgata atgtcgccaa ttttttgcga gctggtggcg atctcctgca tggtgtgagt
     4021 catggtactg acctgaacac cgcccgcctg cgccgttgtc gcggcatttt ttgccagttc
     4081 cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg gcggttaatt gctccatact
     4141 ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta cgacttgaga gatcgttatt
     4201 gcctgcgaca atctccgcga tcccaatgtg catctcatgg cttcccttac gcacatcact
     4261 caccgtccca cgcaaagcct gctgcatggt cttcaggctg gcaaaaatgg cggtgatctc
     4321 attacgacca tataccgcaa tcggacgcgc cagattaccc gcagcaatgc tgtcaaaatg
     4381 gctaccgata atggccagtg gttgaacaat catcttgcgc gtccaccaca gcgcactgct
     4441 gatgtagatc gccgcaacaa taatcatgct gataaacacc agcgccgaaa tctgatagtt
     4501 acgctggctt tgcgcactgg cggcttccag cacatggttg atctccagct gccaggcctc
     4561 aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag
     4621 ttgattactt tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact
     4681 tttctccgtc tctttttgca atgccctgac gtgctcgttg cccgcagtca tcgccataaa
     4741 acttttaaac agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca tcaacgtttt
     4801 aatgtcatcc gccggatagc taagcgctgt cagagtgcct gctttgttca gcgcggtact
     4861 ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat tgctctgctc
     4921 gccgtgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc
     4981 aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg
     5041 attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaaaa ctcgtcaaaa
     5101 tgtttcccag tttggatctt gttcagtaat tcgcagtcgt ggctgtgccg gtggttgctc
     5161 actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg ccagacggaa
     5221 cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagctg cggcggcagc
     5281 tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg aaaccgccaa
     5341 tgcgacttga tcgatgccac ggctctgttc atccgatgcc gatgcaatct cgcccataat
     5401 gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccgg cgctttcgac
     5461 cagcaccgaa ccggtatcaa tgcgcgagac ggagtcttca atgagggctt tgatctcttt
     5521 tgccgccagg gcgctgcgac tggcaagatt acgcacttca cccgccacca cggcaaaacc
     5581 acggccctgt tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga ggatattagt
     5641 ctggaaggca ataccgtcga taacgctgat aatgtcggca attttcttcg aactatcggc
     5701 gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct gggcggtgtc
     5761 ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt
     5821 cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct gctgttcagt
     5881 acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat agatggcatc
     5941 tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct
     6001 ctgcgccagg tcacccattt cactgcgccc gtcaatggtc agggtattcg ccaggttacc
     6061 accggcgatt tcgcaaatgt gagcaataat ttttgccagc ggagtaagca acatacggcg
     6121 aatgccgtac cacgccacca gcagaatcaa taccaccacc agcgcgataa ccgccagttg
     6181 ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat acagtttttc
     6241 actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc cctgggttgg
     6301 ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt cagttaacgc
     6361 tgtgtaatag tttttatatt tttcatcaaa attacggctg gtagcgacca tttcaggtaa
     6421 cggtgccatg cttttgaatt ttttataatg cgtcgcggcc tgggccaatg ttttcctggc
     6481 gctatcgagc aattcaactt tggcgttact ttgttgattg gaggaatcca tcatcatccg
     6541 caccgctgaa cgactcaggt taatgcgcgt ttgcagcatt aaatcccagg ttgacgtcag
     6601 ctcgccctgc tgttcccgta attgattgga aaccacaaag ctcttctggc tatggtgaag
     6661 ggaagaaaaa aacaaactgc cggaaataag ctgtaacagt gcgaataccc ccagcaccat
     6721 taccaacagc gtgactacgc ggatacggtt aatcataagg caccttcctg aaaacaagtt
     6781 gatctcgtta tcggcaagaa ggggggaaac tttattgctg atgccacccg ccgcgaaatt
     6841 gaaataaaaa acccgatgcg cagatcatcg ggttcatttc aattgaggaa atcgggagaa
     6901 ttacgccact tctgacgccg cgctatctaa cagcgccatc tcttcgctgt tcagcagttt
     6961 ttcgatgttc accagaatca acatacggtc gcccagtgcg cccagtccag tgagatattc
     7021 tgttgaaagc gtcacggcaa actctggtgc cggacgaatt tgctccgccg tcaatgaaag
     7081 cacgtctgaa acgccgtcaa ccacgatgcc gaccacccgc tgtccgagat tcaggacgat
     7141 aactaccgtg ttgtcgttat agtccacatc cacctggctg aacttaattc gtaagtcaac
     7201 aatcggcaca ataacgccgc gcagattcgt gacgcctttg ataaacgctg gcgtgttcgc
     7261 aatccgtgtt acctgatcgt agccacggat ctcctgcact ttcaggatat caataccgta
     7321 ctcttcatca ccaagggtaa ataccagaaa ttcctggcct gacggctcgc tggccagctt
     7381 tgttacattc gtcataccgg tcatattgtt acctttttac tcattcaggc ggcggtgttc
     7441 gccatacgtt gttcgcggtt tatcgcctgc aaggcggaga catcaacaat cagtgccacg
     7501 ctgccgtcgc caagaatggt cgcagcagaa atgccgggga ctttgcgata gttactttca
     7561 aggtttttaa ccacaacctg gtgttgacca attaattgat ccaccagcaa ggcgtagcgg
     7621 cgaccgccac tttgtaagat caccacaatt ccctgggtgg cttcggtttt cgcgcccgcg
     7681 acgttgaaca ctttccacag ttcgacgatg ggcagatatt caccccgcac ttccagcacc
     7741 cgctcgccgc cggccagtgg atggagatcg gcttcacggg gttgcagtga ttccataaca
     7801 gcattcagcg gcagaatgaa aacttcatcc gcaacgcgta cggacatgcc gtcgaggatg
     7861 gccagcgtca gcggcagtaa aatgcggatc gtagtgccag taccctgctt cgactggatt
     7921 tcgacatgac cgcccatctc ctggatatta cgtttaacga cgtccatgcc gacgccgcgc
     7981 ccggagacgt cggtgacctg ctctgccgtg gagaagcctg gtgcaaatat cagcattgca
     8041 acttcgtcgt cgctcatgtt ttcgctgaca gtcaaacctt gcgaggccgc ttttgccaga
     8101 attcgctcac ggtttagccc cgccccatcg tcggtcactt caatgcaaat gttgccgccc
     8161 tgatgttcgg cagacagaat taaatttccg acgctgtttt tacctgcggc gagccgtttt
     8221 tctggcagtt caataccgtg atcgaggcta ttgcgtacca ggtgggtcag cgggtcgata
     8281 atgcgttcta tcaggctctt gtcgagctcg gtggaactgc ccaccagcgt cagttctacc
     8341 tgcttgccga gttttcccgc cagatcacgc accagccggg gatagcgact aaagacatat
     8401 tccatcggca tcatgcgaat cgacatcact gattcctgca aatcacgggc gttacgttgt
     8461 aactgcccca tgctggtaat caaatcacca tgattaaccg ggtccagttc gctggaacgc
     8521 tgggcaagca tggactgggt gataaccagc tcgccgacga ggttaattaa ttgatcaacc
     8581 ttttctaccg ctacacggat gctggtggat tcactgctgc gcgtcgtttt ttcccgctcc
     8641 acgcggccgg ttggcgcttg ttcggctgcc agtttaagca caggtggggt agctattttt
     8701 ggcgaaactt ctactgtttc aaaggtaatc tgatcggctt caatcacaaa acagagtacc
     8761 gctgtgatgt catcttcggc gatatcgccc ggtaatattg ccgagagaga atccgcccct
     8821 ttcaccacgt catttaacgt tgtcagatgc cccagctctt cttccagcag gtcgacttcg
     8881 ccggccttca ggcgcgaaag gataattcgt cgcggcaact gactgcgact ttgctcatct
     8941 tgcggttcac ttttggcaac cacacttaat cgggtcactg cggatggcgt ttcgcctttc
     9001 gcttctaatg ccagttgacg caaggcctgg cagatataat cgtagctggc ggcatccggc
     9061 tcttgcgact gtttataagc gtcgagctgt tcttgcatga tgtccttcgt ttccaaaaac
     9121 agattgataa tgtcggtgtt gagttgcatc tcacctcgtc tggcttcatc gagcaggttt
     9181 tccatcagat gcgtggtttc ctgcaaaacg ctgaagccaa aagttcctgc ccctcctttg
     9241 atcgagtggg cagcccgaaa gatggcattc aattgttcgg catctggcgc ttccggctgc
     9301 aaaaccagca gatgctgctc catatcagcc aacagttcgt ccgcttcatc aaaaaatgtc
     9361 tgataaaaat cgcttatatc gatgctcacg ctgtcacctc ggttcggctg atggcattgt
     9421 gggaacactg acctgtggtg caacctcagg tttttctagg gcgcttactg gctcattctg
     9481 gctttcggcg ttttcatgca aaatggcctg ttcggcttgt ttgttcagta ccaacaggct
     9541 gatgcgacgg ttgacggcat catcaggtcc gcgatcgctt aagcgcatcg ttgccgccat
     9601 gccgacgaca cgtaacactt tgccgctatc caaccctccg accatcagtt cgcggcggga
     9661 tgcattggcc cgatcggcag aaagctccca gttgctgtaa cctttctcac cgctggcgta
     9721 gggaaaatca tcggtatgac ctgaaaggct aatacggttg ggaataccgt tcagcacagg
     9781 cgcgatggcg cgcagaatgt cgcgcatata gggttcgaca tcggcactgc cggttctaaa
     9841 catcgggcga ttctggctat cgatgatctg aatacgtaga ccttcctgga ccagatcgat
     9901 tttgagatgg agacgtaacg cccgcagttt cggatcggac tctatcaact ggtcgagatc
     9961 accccgcaat ttccgcaatc gactttgctc catgcgtttt ttcagctctt cgatgttcgg
    10021 ctgcttattc acttccccct ggctttgggt gtaatcatca ccaccgccgg gaattgggct
    10081 ttcactattg gaaatgcgat cgccgcccgt aaccgctgtc gccagtggag tccggaagta
    10141 ctccgcaatc tgaatcaact cttttgggct ggagatggag atcagccaca tcaccagaaa
    10201 aaaggccatc atcgcagtca taaagtcggc ataagcaatc ttccacgatc catgtgctgc
    10261 cccgtggctt ttggctttgc gtcgtttgac gacaataatc ggatgcgctt gattcttcat
    10321 gcttcctcgg ttgtcgtctg ttgttgcgga tttttcaccg cacgcacatg ctcttccagt
    10381 tcaataaacg acggacgttc gctggaatag agcgttttgc gaccaaactc aacggcgata
    10441 ggcggtgcgt aaccgttcag attagaaagc agagtgactt tgacgcactg catcattttg
    10501 ctggtttcgg cgcttttctg acgtaaaaca ctcgctagtg gggaaataaa tccgtaagcc
    10561 aataaaatac cgaggaaagt ccccaccatc gcatgtgcga taagcgcccc cagctcggcg
    10621 gcaggacgat cggctgaacc taacgcgtga acgcccccca ttacagccgc gacaatacca
    10681 aacgccggaa gtgagtcccc gaccagcgcc agactgttcg ccgggacttc tgcctcgctt
    10741 tcgtgcgtct caatctcttc atccatcagg gcttcgattt cgaaggtgtt catgtgaccg
    10801 ctgataatca ggcgcagata atcgacgata aaatcaagca tgacgctatc cgcgaggatg
    10861 cgtgggtagc tggcgaagat ctcgctctca cggggatttt caatatcacg ttccagcgaa
    10921 aacatcccca tctgccgcga tttcgccatc aaccgataaa gcagagccag cagatccata
    10981 tacattgctt tggtgtattt ggagcgacga aacagcaacg gcagcgcttt cagcgtgcct
    11041 ttaatcgctt tgccattatt gccgacgata aacgacccaa tccctgcacc ggcaataatc
    11101 accagttcag cgggttgata gagtgctcca aggcttccac cggtcatcaa ataaccgccg
    11161 aaaactgtac cgagaacaac caggtaacct aataagataa gcacgacatc atccttccac
    11221 tgttgaccat gacaggatgt tcagtcgtca ggcgttaacg cgcgattggg gaaaaaaaaa
    11281 gcagcggtac gtcgttaccg ctgctggaat gttgcgccac accgtatcag ttaaacagcc
    11341 tgtactctct gttcatccag cagttgtggg ataatatcgg caggattctg ggaaagttta
    11401 cgtcttttta ctgcccggga tggcggttga cataagctgc aggcaaagct gccaacaggc
    11461 tggtgagcgt gggtaataaa attgccgccg cagcagttgc agctggaaag ttgcagtaat
    11521 ccactttcaa caaaccgcac caatgtccag gcacgggtta atgccagcag tggtccttct
    11581 tctgcttgtg ggcactgttc aaggtataaa cggtaggctt tgatcaccgc atcgacgcca
    11641 ttacacaaac cggttttcag taaaaactgc catgcattac agaacatcga agcatgaacg
    11701 ttttgttccc aggtcataaa ccagtcggtt gagaatggca gcatgccttt cggcggtggg
    11761 cttccgcgca gttctttata aagttttatc aggcgtccgc gacttaactg tgtttcgctt
    11821 tccagcatct gcaaacgagc gcccagggtg atcaattcca ttgccagctg aatatcccgc
    11881 gcttcctgaa caatgctttt ttcactcatg atcaggccct tttcttgcgc agcgcttctt
    11941 caggctgatt aacatcattc agcaagcgtg ttgagagcat gatgccggta tgaatttgct
    12001 ggagatcgtc aacgcgggaa tcttgcgtca actgagtaat cgtctggtgg ctgtcaaaac
    12061 ggaagtgaca aaccagctga ttggtttctg ccagcttaac catttgcgga ag
//
LOCUS       DAJTSF010000113        11924 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000113 DAJTSF010000000
VERSION     DAJTSF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.113"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..2341)
                     /locus_tag="OO335_003348"
     CDS             complement(<1..2341)
                     /locus_tag="OO335_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6003605.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2275..2898)
                     /locus_tag="OO335_003349"
     CDS             complement(2275..2898)
                     /locus_tag="OO335_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR6003606.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2895..4583)
                     /locus_tag="OO335_003350"
                     /pseudo
     CDS             complement(2895..4583)
                     /locus_tag="OO335_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="OO335_003351"
     CDS             complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="OO335_003351"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6003607.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7506..8228
                     /locus_tag="OO335_003352"
     CDS             7506..8228
                     /locus_tag="OO335_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6003608.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8356..10290)
                     /gene="yfaL"
                     /locus_tag="OO335_003353"
     CDS             complement(8356..10290)
                     /gene="yfaL"
                     /locus_tag="OO335_003353"
                     /inference="COORDINATES: protein motif:HMM:NF007285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR6003609.1"
                     /translation="MMEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMA
                     DSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGD
                     GATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATD
                     GVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGT
                     LLFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQ
                     NNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDP
                     TPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHY
                     TAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADN
                     QNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLE
                     AGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTA
                     VGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAW
                     QKGSDDFAQTAGFLSMTVKW"
     gene            complement(10300..11895)
                     /locus_tag="OO335_003354"
     CDS             complement(10300..11895)
                     /locus_tag="OO335_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HCR6003610.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2757 a         3307 c         2945 g         2915 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagacctt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatcgcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatatagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc cgtttttccc
     2581 gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg
     2641 cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt gaactttcag
     2701 cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
     2761 tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa acagcctgga
     2821 ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc aaatcagcgc
     2881 cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca ggcgacaccg
     2941 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
     3001 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg
     3061 gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
     3121 atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag tgtttgcagg
     3181 gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgccagc
     3241 gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga
     3301 tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc ctgctgagtc
     3361 tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
     3421 aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc gacaaacagc
     3481 ggggtttgca attttgagtc ttcataccag cacagccccg cggcaccgtc taacgcccca
     3541 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa
     3601 tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac cggcgtaaaa
     3661 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact gtgccagccg
     3721 tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg gtagccaaaa
     3781 cccagcaacc tggcgctgac cacgatgcgc aggcgtactg gcgttttttc ggctgtacgc
     3841 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
     3901 gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact ggaaaacacc
     3961 agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata gcgcaactga
     4021 taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt gcttaactag
     4081 ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc actgcgctgg
     4141 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
     4201 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
     4261 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
     4321 aaatcttcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt gggaagctgg
     4381 ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg cagattgttg
     4441 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
     4501 acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa ccgccagcct
     4561 ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg
     4621 ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
     4681 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
     4741 ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt ccgcggcact
     4801 gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct gcagacccac
     4861 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcccacgat
     4921 gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
     4981 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
     5041 ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg cagtacgttt
     5101 accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
     5161 gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat tgcagcccat
     5221 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
     5281 ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa ccagtttgat
     5341 cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt tcttcacagt
     5401 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
     5461 cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac gaccgcgcgc
     5521 gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac cacggctgga
     5581 gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa agtcttcttc
     5641 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt attcagaaag
     5701 cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt cctgggtgat
     5761 cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc
     5821 gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac gatcggcgct
     5881 accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt cgtcgagcag
     5941 tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
     6001 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tctggctcca gccattccgg
     6061 acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca gctgccacgg
     6121 attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
     6181 gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg
     6241 agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt gacgaacaaa
     6301 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
     6361 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
     6421 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
     6481 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
     6541 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
     6601 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
     6661 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
     6721 cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
     6781 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
     6841 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
     6901 ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag
     6961 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
     7021 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
     7081 cgccatacgg acgatcgtgt cataaaccgc cgagtcacca tggggatggt atttaccgat
     7141 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
     7201 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
     7261 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta gctcttcctc
     7321 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
     7381 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
     7441 cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
     7501 tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccacgaa gagatcgcta
     7561 aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc
     7621 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
     7681 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
     7741 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ctgcacgcgc
     7801 tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac
     7861 atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta cccgatccgc
     7921 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
     7981 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa tatattttgc
     8041 gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca gagctgcttg
     8101 gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat tacaacccga
     8161 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
     8221 ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc
     8281 aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc ggcaattgtg
     8341 atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa
     8401 atcatcactc cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt
     8461 gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac tgatagtgct
     8521 gccatcttct tcaatttccg tcgcatgggg atcgtgatag taattcagat ctaatgttgg
     8581 tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct gaatatcatc
     8641 cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc
     8701 ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta accactgata
     8761 cccagcttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc catcttcatg
     8821 ttcggaaaca tcattgttaa accacgcgta ttgcagccag ctatccagcc aggccccctg
     8881 cttctgatta ccgtgctgat accagcttga ggtcagccca acggcataac cgtggttctg
     8941 gttatcggcg cgagttccgg tcatattcga gcggctgtcg ccctgattat cactgtagcc
     9001 accaaccgcc ccaagcatcc actcgccatc cgtgccccag cgcccgctaa acaagccgcc
     9061 gctaagctgc accgtagaag tgtcttcctg ctgagccagt tgccctgccg ccgtgtaatg
     9121 ataacgtccg ccgataacac gtaaattcag cgtctgacca tcgccaccag cgtgatcgcg
     9181 tcgctccatc ataaacgcct gatttgccgc ccgcaggtta ttgagataac cgccaacttt
     9241 ggcattcagc accggctggt aagcaggcgt aggggccggg tcgggggttg ggtcaggatc
     9301 cggcgtggga tcaggatcgg gagtcgggtc tggctcaggt ggcgatggcg gcgttacttc
     9361 ttgcgatcgc agataccagt cgttgttatc ttccactagc gtgtagtcat acgctcccat
     9421 gttgacgtag ccactgccag ccagactgaa ctgtgcattg ttttgaaact gcgtgggatc
     9481 agctgcgaaa tcaaccactt taatgcctgt cgatgtcggc tcaccaatcc ctgtaatgga
     9541 gttaaccacc acagttgtgt tgccagcagt attaccgttc atcaccaatt gatcgcttac
     9601 cgagtcatcg ccgtttaatt cgctgtcgaa tagtagcgta ccgccgccgg tataatcacc
     9661 gtttaccgtc agcgtatcgc cagcgacgcc attttgcagg ctaaccgcac cggacgtatt
     9721 ggtcagatca ccattaaccg tcacgtcagc aagataggtc aggctgtcag tttcaaggtt
     9781 ggtattaagc tcgcctgtca acgtcacgcc atcggtagca tttaccagcg tcccatcgcc
     9841 gttaaatagt gaggcattaa gggcgacgga agtatcctgc ccggtcagac gcaaaaccgt
     9901 accatcgctg atgtcgatag tgccgctgga agtagcatca attgactgaa tatcctgatc
     9961 cgcgccagta acgaacgttg ccccatcacc aacccacagt gacgaagcat aaggaaagat
    10021 atccgcaaca tcgcctttca gggtgccttc ttcgacacgt accgccgact gcgttgtacc
    10081 gctggcggtc agctccagcg tacccgcccc cgttttagta aatgtgctac cctcatcctg
    10141 atgctgcccg ctgctgtcag ccatcagtgc gccccactgc gtgtctaccc ccgcatcaac
    10201 tgccacttca ccgtcggcgc gcatttcaat atcacgccca tgctcagtcg tcgtagagcg
    10261 agtatcgaca ttggcagtaa tctccatcac gctttcttgt tacttatcgg taaagataac
    10321 ctggcggtta tagtgggtat ctccaagctg cgagttgtcg ctcaccatca gcgtaccgga
    10381 ggcgatttgt gttgtgccca aataactatt attgtttgcc agacttacct gcccgtcgct
    10441 cgcatcgacc accacgtaga cgccatcttt cccgtcgccg ccgatatgca aattgtcgcc
    10501 acctgcaaga tctaccacat cctgactgcc gataaccgtt ccgctgctgc cactgacttc
    10561 aaggccatca ttgtatgatg tgccgctctg ccaagtggag aaatcagaga gatcgagcac
    10621 accgccgttt aacacgatcg actgcggatc gtcctggaga gcggtaagat ctgccgcgtc
    10681 gccttccagc gaaagcaccg caccatcatc aacgactata tcgccggtta gcgccatcga
    10741 ctgcgcccct gccagcacgt agctgccgtt ttgcgcaatg gtgagctgac cagcaccttc
    10801 gatgatgcca gcaaaactgc cctgattaac agtaacgttg ccaccagcat cgatatttaa
    10861 agtgccattc tgaaagcccg tcaatgcgtg cacaaaagtt tgttgggtcg agccaacgtt
    10921 tagctcagcc tgattctgat actgatcaat actccctatc gtcagaccgt agcagtcttg
    10981 cggatcgtcc tggcaatgcg tatcgccgac attcatcagg gagttgctgc gacccagggt
    11041 aacttcaccg ttttcaatct gcatctcacc agtaaagtca ttgttatctg cattaagtac
    11101 cagatcgccg gaacctgttt tggtgattaa cccggtacca gcaatagagt caacagctcc
    11161 gtcattctct gtattgccga taaccagcgt ttttccgtcg gcaatatcaa aggtaacttc
    11221 acttaagccg agatacataa agccacccgc cgcagtggag ggaccatctc catagcctgc
    11281 tgcgctattg ttctcatcga ctaacacgcc gccgttctgg ctgtagctgt catcaacaga
    11341 aatatcaata agatagggag ccgtcgcgct attggtatat atcgccccgc cataaccttc
    11401 ggctgtgtta tttgtaaagg cggtgttatt tattatcgta taacctgaag gatgcttgct
    11461 gtcgctatta ttatcggtaa catcgattgc cccgccatcg ccatcactgt aacttgttga
    11521 tgtatatgcc tggttattgt taaaaacatc atcacttaaa tagacgtcat tattaatggt
    11581 ataaattgcg ccacctttgc catcattagc gatattattg atggcgttag tgacacgtaa
    11641 atcgacggca ccagtatcat tagtaccaga agaatagatt gcgccaccat agccgcctgc
    11701 gacgttaccg gaaaaaataa catcagtaag attcagcgtt gagttttctt tagcaaatat
    11761 ggccccgccg ttattatatt ctcctgtaac ggtgttattg gcaaacagag tcattccagt
    11821 ttcattttct ggtaatagtg aaaattccgc tccttgttgc aaaaatacgg ccccaccgct
    11881 ggcattattg gtcatatccg aaaaaaccag ccattgccca tcgg
//
LOCUS       DAJTSF010000114        11682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000114 DAJTSF010000000
VERSION     DAJTSF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.114"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..346
                     /locus_tag="OO335_003355"
     CDS             <1..346
                     /locus_tag="OO335_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6003611.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
     gene            complement(463..738)
                     /gene="yjfN"
                     /locus_tag="OO335_003356"
     CDS             complement(463..738)
                     /gene="yjfN"
                     /locus_tag="OO335_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6003612.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="OO335_003357"
     CDS             complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="OO335_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6003613.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1398..2147
                     /gene="yjfP"
                     /locus_tag="OO335_003358"
     CDS             1398..2147
                     /gene="yjfP"
                     /locus_tag="OO335_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6003614.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="OO335_003359"
     CDS             complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="OO335_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6003615.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="OO335_003360"
     CDS             complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="OO335_003360"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6003616.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4426..5823
                     /gene="ulaA"
                     /locus_tag="OO335_003361"
     CDS             4426..5823
                     /gene="ulaA"
                     /locus_tag="OO335_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR6003617.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5839..6144
                     /gene="ulaB"
                     /locus_tag="OO335_003362"
     CDS             5839..6144
                     /gene="ulaB"
                     /locus_tag="OO335_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6003618.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6154..6618
                     /gene="ulaC"
                     /locus_tag="OO335_003363"
     CDS             6154..6618
                     /gene="ulaC"
                     /locus_tag="OO335_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR6003619.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6632..7282
                     /gene="ulaD"
                     /locus_tag="OO335_003364"
     CDS             6632..7282
                     /gene="ulaD"
                     /locus_tag="OO335_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6003620.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7292..8146
                     /gene="ulaE"
                     /locus_tag="OO335_003365"
     CDS             7292..8146
                     /gene="ulaE"
                     /locus_tag="OO335_003365"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6003621.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8146..8832
                     /gene="ulaF"
                     /locus_tag="OO335_003366"
     CDS             8146..8832
                     /gene="ulaF"
                     /locus_tag="OO335_003366"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6003622.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8961..9236)
                     /locus_tag="OO335_003367"
     CDS             complement(8961..9236)
                     /locus_tag="OO335_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6003623.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9563..9958
                     /gene="rpsF"
                     /locus_tag="OO335_003368"
     CDS             9563..9958
                     /gene="rpsF"
                     /locus_tag="OO335_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6003624.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9965..10279
                     /gene="priB"
                     /locus_tag="OO335_003369"
     CDS             9965..10279
                     /gene="priB"
                     /locus_tag="OO335_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6003625.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10284..10511
                     /gene="rpsR"
                     /locus_tag="OO335_003370"
     CDS             10284..10511
                     /gene="rpsR"
                     /locus_tag="OO335_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6003626.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10553..11002
                     /gene="rplI"
                     /locus_tag="OO335_003371"
     CDS             10553..11002
                     /gene="rplI"
                     /locus_tag="OO335_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6003627.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11073..11636)
                     /locus_tag="OO335_003372"
                     /pseudo
     CDS             complement(11073..11636)
                     /locus_tag="OO335_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2767 a         2871 c         3087 g         2957 t
ORIGIN      
        1 cggcaatatt atgtgcctgg atgtgttgcg cgttctcaat aagcaagcgg gcgtatacga
       61 cttattgtcg gaagcatttg tggaagtgaa agggcaggat cgctattttg atcgcgcggt
      121 tcgtcgttta cagcagcagc tgcgtaagcc agctgaagaa ctggggcgag agattactca
      181 tcagctattc ctgctgggct gcggtgcgca aatgttgaaa tatgcttctc cgccaatggc
      241 gcaggcgtgg tgtcaggtga tgttagatac gcgcggcggc gtacggttgt cagagcagat
      301 ccagaatgat ttattgctgc gggcgacggg gggagtgtgt gtgtaagcgt atacgactga
      361 tgcgacgctg gtttcgatta actaaatgaa atatgtgaaa attgtaggcc ggacaaggcg
      421 ctcgcgccgc atccggcatt gttcatttgc tgtagatgac actcatgcat acagtatcgc
      481 ctgtacgcgc cagttatcag gctgctggtc ttcgtacatg gtaataatgc ggtaccatga
      541 cgcaccttgt tcatcggcct ttaacgccac gatacgttcc acgtcctgcg gatcccctga
      601 aatattgcta actgagatct gacctatttc attcaggccc gtcacgcagt cagcactggc
      661 gaattctgca gactgtgccg tggcgctcgc aggataagtc actaattgca gcgagggtga
      721 ggcaagtaat tgtttcatcg tcagctccat ttttcgtttc cccacatgtt gcagggcatt
      781 ccatcgcggt aataaacgcc caagcttcca gccggtaatt tattgtgcac aggtaaacgc
      841 atagggacgc aaagcaatgt aaagctgact atgtaagttg tctgatttat ttacggtaca
      901 aaatggcttg tgaataccac tgtcctgtca cgatggtttc gtccaccatg acgacaacgt
      961 aataatcagc ttttgcagcg acagcttttg ctctgatttc tgctaatgcg tcatccggag
     1021 aaccccgaac catcgtgctt acgctaccta ttcgctgtaa cccttgcgtc tggtcgcggc
     1081 gaatctcttg cggatggtcc gttactggcg gtgctggctg tggcgtacct tgcagtgcgc
     1141 tacaggcact taacatcaac accaataata aactggcaaa ccggtaaata acgctattac
     1201 gtttcctgct aaccatagtg tagtgcctta ttaatttaac tttgggggaa tgttcccgaa
     1261 ttgttacctg gtacgcgatt ttcgaatgaa aacgtgacga aagcgtaacc cacatctcaa
     1321 cattatgaac taataccgcc agcagaaact tgtgctaagg atttcttcat gtcataccag
     1381 acaaaaagga gagacagatg attgaaatag aatcacgcga gctggcagat attcccgttc
     1441 ttcatgctta tcctgtcggg caaaaagata ccccgttacc gtgcgtaatt ttttatcacg
     1501 gctttacttc atccagcctg gtgtatagct attttgccgt tgcgctggcg caggctggtt
     1561 tgcgggtgat catgccggat gcgcccgatc acggtagccg ttttagtggc gacgaagcgc
     1621 ggcggttaaa tcaattctgg caaatcttgc tacaaagtat gcaggaattc actactttac
     1681 gtgcggcaat agccgaagaa aactggctgc ttgatgaccg tctggcagtc ggtggcgcgt
     1741 cgatgggcgc gatgacggca ctgggaatta ccgctcgcca ccccacggtg aaatgtaccg
     1801 ccagcatgat gggatcgggc tattttacat cgctcgcccg ttcactgttt ccaccgctga
     1861 tacctgaaac ggcagcacag cagaatgaat tcaataaaat tgtcgcgccg ctggcagagt
     1921 gggaagcgac aaaccacctg gaacaacttg gggacagacc tctactgctg tggcatggcc
     1981 tcgacgatga cgttgtgcct gccgacgaat cactacgttt gcagcaggcc ttaagcgaga
     2041 cgggacggga taaactgcta acctgttcat ggcagccagg cgtgcgtcac cgcattacgc
     2101 ctgaggcgtt agatgctgcc gtgacatttt tccgccagca tctttaaaca cgcagaatgc
     2161 tgaccccttg cgcttccagt tgttgcagga tttccgggtt agcatttttg ccggtgatca
     2221 gcatatcgat ttgatcggca cggctaaaaa gcattcccgc gcgttcgcca atcttactgc
     2281 tatcaaccag taccaccagt ttccctacca cgctcagcat cttctgctct gccattgctg
     2341 tcagcatatc ggttttatac aacccttctg cggtcagccc ttttccgctg gtaaacatcc
     2401 agtgcccggc atagagactg ttttcgctgc cctgcgggct taaagtgatg gactgacttt
     2461 tgttgtactg tccacccata atgatcacgc tgtcatgttc ttgatcgatc aggtaatttg
     2521 ccagcggtag ataattagtg atgatttgca ctggcttgcc acacatttcc cgcccaagca
     2581 gaaacgcggt ggagccgcag ttgatgacta cgctttcgcc cggattaacc agctgcgagg
     2641 ccgctttagc aatacgtact ttttcatcgt gattctgcgc ctgatgcaga ttcatcggcg
     2701 tccagcgcgg gcgctgttgg gtaatagctt ctgcgccatt gcgcaccttt ttcagtttgc
     2761 cgctttcgcc aagtttattg atatcgcgtc gcgcagtggc aggcgaaatt cccagacgct
     2821 caacgacttt ctcaacggtc acaaagccca attgtgcgag catttccagg aggatttgat
     2881 gtctttgtgc ttcagtcatg agctattccg ataaaatttg atttttttag atgatatttg
     2941 aaatagccag gaaatactac gccggatagc gcgaacttct ccacgctatc cggtataaac
     3001 gacagattac aggaatgact tgaacggcag atccggttca atagtgaagc aatcatcgaa
     3061 accgcgcgga tagtggtact cgaagttgtc tttatccagc ggccaggtaa atttgccgcc
     3121 aacctgccag ataaacggct tgaagccata cttcagacga tcttttttga tctcccacag
     3181 cacgcggatc tcttgcggat cggcctggaa gtttgaccag atgtcgtggt ggaacgggat
     3241 aaccactttc gcattcaggg cttcacccat acgcagcata tcggcgctgg tcattttgtc
     3301 ggtaataccg cgcgggttct caccatacga tcctaacgcc acatcgatct gatgttcgtt
     3361 accgtgcttc gcataatagt tagagtagtg ggaatcgcca ctgtgatata gggaaccgcc
     3421 aggggttttg aacaggtagt ttactgcgcg atcgtccatg ccatctggca gtacgccagc
     3481 cgctttttga tcggcaggca gggtgatcag tgcagtacgg tcgaaagcgt caagcgcatg
     3541 aatttcaatg tctttcactt ttacaacatc gcccggtttg accacgatgc aacgctcttt
     3601 cggtacgccc cagccaatcc acaaatcaac gcaggttttc ggtccaatga atggtacgtc
     3661 atcagcacag ttctgcatca cggcagcagc gacgttaacg tcgatatgat cgttgtgatc
     3721 gtgagtcgcc agtaccgcgt cgatctggcg aatcgcaaac ggatcaagaa caaacggggt
     3781 ggtgcgtagg tttggctgca gttttttcac gccagccatg cgctgcatct ggtgaccctg
     3841 tttcattaac gggttaccgt gactttgttt gccagtgccg caccagaaat caacgcaaac
     3901 gttggtgcca ccttctgatt tcaaccagat cccggtgcag cctagccacc acatcgcaaa
     3961 tgtgccaggg gcgacctgct cttgttcaat ttcttcattc agccagctac cccactccgg
     4021 gaaagtgctc aggatccagg attctcgggt gatacttttc actttactca tcgccattta
     4081 ccttcatgat agttcaattc gaatcaacat gtgattggtt ttgattaatc ctgacactat
     4141 tccttcagga aggtaatgac cattttttga ctttttgcca gggaagttgt tgttgatttt
     4201 gagtggggaa agatttaatg gaatgtgtag ttaaattaac taaatgaatt taaataaata
     4261 attgtttcgt tgtgtgaatc ccactctatc catgtggaat tatttgcggg tcgcgtcaca
     4321 tttaatcata aataatcttg ttgtgattac ttttgaaaat tagagtgagt gcacaacatt
     4381 ccgggtgtgc ggaatacccg gttacctctt cttcaggaga tcgttatgga gatcctctac
     4441 aacatcttta ccgtgttttt taaccaggtc atgaccaatg ccccgttgtt gctgggtatt
     4501 gtgacctgtc tgggctacat cctactgcgc aaaagtgtca gcgttattat taaaggcacg
     4561 attaaaacca taattggttt catgttgttg caggcagggt ccggcatcct caccagcacc
     4621 ttcaaaccgg tggtggcgaa aatgtccgaa gtctacggca ttaacggcgc aatttccgat
     4681 acttacgcgt cgatgatggc aaccatcgac cgcatgggcg atgcctatag ctgggtaggt
     4741 tacgcggtgt tgttagcgct ggcgctgaac atctgttacg tgctgctgcg tcgcattacc
     4801 ggtattcgta ccatcatgct gactggtcat atcatgttcc agcaggcggg gctgattgcc
     4861 gttacgttgt ttattttcgg ctactccatg tggacaacca ttatctgcac ggcgattctg
     4921 gtttcgctct actggggcat tacctccaac atgatgtaca agccgactca ggaagtgacg
     4981 gacggctgcg gtttctccat cggtcaccag caacagtttg catcattaat tgcctataaa
     5041 gttgcgccgt tcctcggcaa aaaagaggaa agcgttgaag acctcaaact gccaggctgg
     5101 ctgaatattt tccacgacaa catcgtctcc acggcgattg tcatgaccat cttctttggt
     5161 gccattctgc tctccttcgg tatcgacacc gtgcaggcga tggcaggcaa agtgcactgg
     5221 acggtctata tcctgcaaac cggtttttcc ttcgcggtgg cgatcttcat catcactcag
     5281 ggtgtgcgca tgtttgtggc ggaactctct gaagcgttta acggtatctc ccagcgcctg
     5341 atccctggtg cggttctggc gattgactgt gcggctattt atagcttcgc tccgaacgcc
     5401 gtggtctggg gctttatgtg gggcaccatc ggtcagctga ttgcggttgg catcctggtc
     5461 gcctgcggtt cctcgatcct gattatccca ggctttatcc cgatgttctt ctccaacgcc
     5521 accatcggcg tgttcgctaa ccacttcggc ggctggcgtg cggcgctgaa gatttgtctg
     5581 gtgatgggga tgatcgaaat ctttggttgc gtctgggtgg tgaaacttac cggtatgagt
     5641 gcctggatgg ggatggcgga ctggtcgatt ctggcaccgc cgatgatgca gggcttcttc
     5701 tccatcggta tcgcctttat ggccgtcatc attgtaattg cactggctta tatgttcttc
     5761 gctggccgcg cactgcgcgc agaagaagat gcagaaaaac aattggcaga acagtctgct
     5821 taataaggag ttttgattat gaccgtacgt attctggctg tgtgtggcaa cggacaaggc
     5881 agttccatga tcatgaagat gaaagtggac cagtttttaa cccaatcaaa cattgaccac
     5941 acggtaaata gctgcgcggt tggcgaatac aaaagcgagt tgagcggcgc ggatatcatc
     6001 atcgcttcta cgcacattgc gggcgaaatc accgtgaccg gcaacaaata cgtggttggc
     6061 gtgcgcaaca tgctctctcc tgccgacttt ggcccgaaac tgctggaagt gatcaaagcg
     6121 catttcccgc aggatgtgaa gtaaggacgc gccatgaaat tacatgattc gctggcggag
     6181 aataaatcca tccgcctgca ggctgaagct gagacatggc aggacgcagt aaaaatcggc
     6241 gttgacctgc tggtggcggc ggatgttgtc gagccgcgtt actaccaggc gattctggat
     6301 gcggttgaac agcacggtcc gtactttgtg ctcgcaccag gcctggcaat gccgcacggt
     6361 cgcccggaag agggcgttaa gaaaaccggt tttgcgctgg tgacgctgaa aaagccgctg
     6421 gagttcaacc acgaagataa cgacccggtg gatatcctca tcaccatggc ggcggtcgat
     6481 gccaacacgc accaggaagt gggcatcatg cagatcgtca acctgtttga agatgaagaa
     6541 aatttcgacc gtttacgtgc ctgccgtacc gagcaggaag tactggatct cattgaccgc
     6601 accaacgcgg cagcttaaga aggaattgaa gatgtcatta ccgatgttgc aagtcgcgct
     6661 ggacaaccag actatggata gcgcctacga aaccactcgc ctgattgccg aagaagtcga
     6721 cattatcgaa gtgggtacca ttctgtgcgt gggcgaaggc gtgcgtgcgg ttcgcgatct
     6781 gaaagcgctc tacccgcaca aaatcgtgct ggcagacgcc aaaattgccg atgcgggcaa
     6841 aattctttct cgtatgtgct tcgaagccaa cgctgactgg gtgaccgtga tttgttgtgc
     6901 ggatatcaac accgccaaag gtgcgctgga cgtagcaaaa gagtttaacg gcgacgtgca
     6961 gatcgaactg accggttact ggacctggga acaggcgcaa cagtggcgcg acgcaggtat
     7021 tcagcaggtg gtttatcacc gcagccgtga cgcgcaggcc gctggcgtgg cgtggggtga
     7081 agccgatatc actgcgatca aacgtctttc cgatatgggc ttcaaagtca ccgtcaccgg
     7141 tggcctggcg ctggaagatc tgccgctgtt caaaggcatt ccaattcatg tgtttatcgc
     7201 cggtcgcagc atccgtgatg ccgcttctcc ggttgaagcc gcgcgtcagt tcaaacgttc
     7261 catcgccgaa ctgtggggct aaggagcgga tatgttgtcc aaacaaatcc cgcttggcat
     7321 ctatgaaaaa gcgctccccg ccggggagtg ctggctggaa cgcctgcgac tggcaaaaac
     7381 gttgggcttc gattttgtcg agatgtcggt agatgaaact aacgagcgcc tgtcgcgcct
     7441 tgactggagc cgcgaacagc gtctggcgct ggtgaatgct attgttgaaa ccggcgtgcg
     7501 tgtgccgtcc atgtgccttt ctgcccatcg tcgtttcccg ttgggcagcg aagatgacgc
     7561 ggtgcgggcg caggggctgg agattatgcg taaagccatc cagttcgccc aggacgttgg
     7621 cattcgcgtg atccagctgg cgggctatga cgtttactat caggaagcca ataacgaaac
     7681 gcgtcgtcgt ttccgtgacg gcctgaaaga gagcgttgag atggcgagcc gcgcgcaggt
     7741 gacgctggcg atggagatca tggattatcc gttgatgaac tccatcagca aggcgctggg
     7801 ttacgcgcac tatctcaaca acccgtggtt ccagctctac ccggatatcg gcaacctgtc
     7861 ggcgtgggac aacgatgtgc agatggagtt gcaggccgga atcgggcata tcgtcgcggt
     7921 gcatgtgaaa gacaccaaac cgggcgtctt caaaaacgtg ccatttggcg aaggtgtggt
     7981 ggatttcgaa cgttgcttcg aaacgctcaa acagagtggc tattgcgggc cgtacctgat
     8041 tgagatgtgg agcgaaacgg cggaagaccc ggcggcagaa gtggtgaaag cgtgtgattg
     8101 ggtgaaagcg cgcatggcca aagcaggcat ggtggaggcg gcataatgca aaagctaaaa
     8161 cagcaggtgt ttgaagccaa catggatctg ccgcgctatg ggctggtgac ctttacttgg
     8221 ggcaacgtca gcgctatcga ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtt
     8281 gcctacgaaa ccatgaaagc agacgatatg gtggtggtcg atatgagcgg caaggtggtg
     8341 gaaggggagt atcgcccatc ttccgacact gcgacgcatc tcgaactcta ccgtcgttac
     8401 ccgtcgcttg gcggcattgt ccatacccac tccactcatg ccaccgcatg ggcgcaggcg
     8461 gggctggcga tcccggcatt aggcaccacg cacgctgact acttctttgg tgatatcccg
     8521 tgtacgcgtg gattaagcga agaagaggtg cagggtgagt atgaactgaa cactggcaaa
     8581 gtgattatcg aaacgctggg caacgccgag ccgctgcata cgccgggaat tgtggtgtat
     8641 cagcacggac cgttcgcctg ggggaaagat gcccacgacg cggtgcataa cgcggtagtg
     8701 atggaagaag tggcgaaaat ggcgtggatt gcgcgcagta ttaatccaca actcaatcat
     8761 atcgacagct acctgatgaa taaacacttc atgcgtaagc acggtcctaa cgcttattac
     8821 gggcagaagt aaaacacgcg ctgcggaaat ttccttcctc gggagataac tggtctaatt
     8881 ccgcagccgt ttttcaaaaa aagccccctg cgaagggggc aaagcaaact atggcaatgt
     8941 ttcgttggtt atacctggtg ctagcgataa atatccgcgc tggcgtgcat attgccgtta
     9001 ctccccggtt cccgcatcag aattacgtgg tagtacgttg cgccttgcgc atctgtttct
     9061 tcatttaatg cctgacgtgc ttcgctttca gtggcgaaat tatgattgat ataaatcacg
     9121 cctaagcttt gcacatcgtc catatttctg gcctggtggt tattaatttc aatggctgcc
     9181 catgtatttg cacttagcaa aagcacagcc agaagggcta aaactcgact gaacatagat
     9241 acctcctcga cggctgactt tgtgtgctct cctctgtgat gatcttctga tttaatttta
     9301 atcaatgata aagaagttga tggtgaccat ttctgatgca gttgttcaaa aaaacactgt
     9361 gacggaatgt gatgaacttc aaatcagcgg tttagaggtt atttgcgaaa ggggagattt
     9421 atttctgctc cgcccttgag tttagcgggg tatgccagta ctataacgcg tcatttttca
     9481 gccgaccttt aacacgttcc ttgcctcccc gggattcggc tgacccagac aggaggctga
     9541 ataatccgta aggagcaatt cgatgcgtca ttacgaaatc gtttttatgg tccatcctga
     9601 ccagagcgaa caggttccgg gcatgatcga gcgctacact gctgccatca ctggtgcaga
     9661 aggcaagatc caccgtctgg aagactgggg ccgccgtcag ctggcttacc cgatcaacaa
     9721 actgcacaaa gcacactacg ttctgatgaa cgttgaagct ccgcaggaag tgatcgatga
     9781 gctggaaact accttccgct tcaacgatgc cgttatccgc agcatggtta tgcgtaccaa
     9841 gcacgctgtt accgaagcat ctccgatggt taaagcgaaa gacgagcgcc gtgagcgtcg
     9901 cgatgatttc gcaaacgaaa ccgctgatga tgctgaagct ggggattctg aagagtaatt
     9961 tctgatgacc aaccgtctgg tgttgtccgg caccgtgtgc aggactcccc ttcgaaaggt
    10021 cagtccatca ggaattcctc actgccagtt cgtgcttgag catcgttctg tgcaggagga
    10081 agccggcttt caccggcagg catggtgtca aatgcccgtt attgttagcg gacacgaaaa
    10141 ccaggccatt actcacagta taacggtcgg cagtcgcata accgttcagg ggttcatttc
    10201 atgccacaag gcaaagaacg gactgagcaa aatggttttg catgccgagc agattgaatt
    10261 gatagattct ggagactagc catatggcac gttatttccg tcgtcgcaag ttctgccgtt
    10321 tcaccgcgga aggcgttcaa gagatcgact ataaagatat cgctacgctg aaaaactaca
    10381 tcaccgaaag cggtaagatt gtcccaagcc gtatcaccgg tacccgtgca aaataccagc
    10441 gtcagctggc tcgcgctatc aaacgcgctc gctacctgtc cctgctgccg tacactgatc
    10501 gccatcagta atcggtcacg gtccattaat acgactttga gaggataagg taatgcaagt
    10561 tattctgctt gataaagtag caaacctggg tagcctgggt gatcaggtaa acgttaaagc
    10621 gggctatgct cgtaacttcc tggtaccgca gggtaaagct gttccagcta ccaagaaaaa
    10681 cattgaattc ttcgaagcac gtcgcgctga actggaagct aaactggctg aagttctggc
    10741 agctgctaat gctcgcgctg agaaaatcaa tgcactggaa actgttacca tcgcgtctaa
    10801 agctggcgac gaaggtaaac tgttcggttc catcggtact cgcgacatcg ctgacgctgt
    10861 aactgcagct ggcgttgaag tggctaagag cgaagttcgt ctgccgaacg gcgttctgcg
    10921 taccactggc gaacacgaag tgagcttcca ggttcacagc gaagtattcg cgaaagtgat
    10981 cgtaaacgta gtagctgaat aattcgttat tcaacgagac gtaaaaacgc cgaccattgg
    11041 tcggcgtttt gctttctatt tttcgtcagg cattaatttc gcaagtagat ccattcacca
    11101 ttctcttgtg tccataagtc aggatggttt gctggcgtta gatgcatacg atcactcctt
    11161 atgtctcggt cctgcggcac tcgctcgaaa cctaaacccg agtagattgc ggctgaattt
    11221 gataaggaac gtagaccaat tttcaaatca tggggtaaat cagaatacgc gtttagcaat
    11281 gcagcaatga tgaatccgag ggcattagaa acgcctgtaa cagtgtgaag ttggtcgata
    11341 taaagatcga ttgttggtag ttcagatgag cagcaattag gcatttgggc aggaagggtt
    11401 acaacgtatc ctatcgtttg gccggtatag tctggagtac tataaccagg tcccaaaaca
    11461 acctcgtgta ttgtgtattt actgttgggt tcgaaggatt cgacatcttg caataaatat
    11521 ttcagttctg cggttaacct cgcacccgcg taagctttat tgtatttctg ggcgtgaata
    11581 ttatctaatg tagcttcatg gttccatcca aaagaattat caccattcat tgttactgaa
    11641 tcgccacggg tttaacagac acctcagagt catttaagat gg
//
LOCUS       DAJTSF010000115        11655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000115 DAJTSF010000000
VERSION     DAJTSF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.115"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            597..794
                     /locus_tag="OO335_003373"
     CDS             597..794
                     /locus_tag="OO335_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003628.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="OO335_003374"
     CDS             complement(744..1937)
                     /locus_tag="OO335_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6003629.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="OO335_003375"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="OO335_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6003630.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="OO335_003376"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="OO335_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6003631.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="OO335_003377"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="OO335_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6003632.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="OO335_003378"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="OO335_003378"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6003633.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="OO335_003379"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="OO335_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6003634.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="OO335_003380"
     CDS             complement(10573..10716)
                     /locus_tag="OO335_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003635.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10955..>11655
                     /locus_tag="OO335_003381"
     CDS             10955..>11655
                     /locus_tag="OO335_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6003636.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQW"
BASE COUNT         2956 a         2897 c         2992 g         2810 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggcaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tggga
//
LOCUS       DAJTSF010000116        11208 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000116 DAJTSF010000000
VERSION     DAJTSF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.116"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..610)
                     /gene="hdhA"
                     /locus_tag="OO335_003382"
     CDS             complement(<1..610)
                     /gene="hdhA"
                     /locus_tag="OO335_003382"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR6003637.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(722..1750)
                     /gene="malI"
                     /locus_tag="OO335_003383"
     CDS             complement(722..1750)
                     /gene="malI"
                     /locus_tag="OO335_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6003638.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1925..3517
                     /gene="malX"
                     /locus_tag="OO335_003384"
     CDS             1925..3517
                     /gene="malX"
                     /locus_tag="OO335_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6003639.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3527..4699
                     /gene="malY"
                     /locus_tag="OO335_003385"
     CDS             3527..4699
                     /gene="malY"
                     /locus_tag="OO335_003385"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6003640.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4803..5804
                     /gene="add"
                     /locus_tag="OO335_003386"
     CDS             4803..5804
                     /gene="add"
                     /locus_tag="OO335_003386"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6003641.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5840..6880)
                     /locus_tag="OO335_003387"
     CDS             complement(5840..6880)
                     /locus_tag="OO335_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6003642.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7123..7248
                     /gene="blr"
                     /locus_tag="OO335_003388"
     CDS             7123..7248
                     /gene="blr"
                     /locus_tag="OO335_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6003643.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7521..7736
                     /gene="ydgT"
                     /locus_tag="OO335_003389"
     CDS             7521..7736
                     /gene="ydgT"
                     /locus_tag="OO335_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6003644.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7822..8262
                     /locus_tag="OO335_003390"
     CDS             7822..8262
                     /locus_tag="OO335_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6003645.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8339..8920
                     /gene="rsxA"
                     /locus_tag="OO335_003391"
     CDS             8339..8920
                     /gene="rsxA"
                     /locus_tag="OO335_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6003646.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8920..9498
                     /gene="rsxB"
                     /locus_tag="OO335_003392"
     CDS             8920..9498
                     /gene="rsxB"
                     /locus_tag="OO335_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6003647.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9491..>11208
                     /gene="rsxC"
                     /locus_tag="OO335_003393"
     CDS             9491..>11208
                     /gene="rsxC"
                     /locus_tag="OO335_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6003648.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLE"
BASE COUNT         2606 a         2885 c         2823 g         2894 t
ORIGIN      
        1 ctggtgtaat aacggatttc agggcatcgg ttaatattgc ccccggcgca atgctattca
       61 cccgaatatt tttttcaccc aggtcaaatg ccatatttct gaccagatga ctggccgcag
      121 ctttagatga tgcataggaa gtcatgttta tatttttatt ttctgccgcc atagaagtga
      181 tggtcagaat aacgccacct ccattttttt ccatttctgg cgcaacaagt tgtgacagat
      241 ggaaaaaaga aaacacattc agttcataag cacggcgaaa atctgccatt ggcatatcaa
      301 acggtttagg accaccgcca ccggcgttgt taaccaggat atcaacttta cccagattac
      361 tgacggcaaa gtctgccagt gcagagagtt cctgttcgga agtaatatca caacgccagg
      421 caaatgcctg accatccagt tgttgaattt cgtctacaac atggttagct gcgtcggcgt
      481 taatatcact gaccaccaca gatgcgccag ctgtcgcgaa tgtaatggcg atttctttac
      541 caatacctgc acccgcacct gtgatgatgg cgcattttcc gtcgagtctc aggttgtcag
      601 aattaaacac ataaacctcc tgtgaacttc agtaaaaata aaaggcaccg cctgtgtgcc
      661 acatttttta tcattaacct tgaggaaagg acactaccaa gtatagcaga taaagagagg
      721 attatttcgc cgcaatgagc cgggcgggaa taataagatt gcgtgaatgc gtctcttcat
      781 gggtgatttt ttgcatcatg cgatccgcaa gtgtgatacc aagttcccgc gctggcgtgc
      841 tggcccaggt aacaggtata tcatcaagtg tggttggtgt cgcatcggta aatgccgcca
      901 gcgaaacctg ttgctcaaag taacgatcga ctccgctttc cccgctttgc cgccctgctt
      961 tcagcaaacc aaaccatgcc cccatcgcaa tagtttcgtt atagcaaacc acggcactga
     1021 tggtcgggtt atgacgtaaa agcgccgtga tagcttccgc ggcttgcttc tggctggaag
     1081 tgcactccaa cacccaatcg ctgtgaaacg gcaggccaaa ttttagtaga gttgcacaat
     1141 agccccccac ccgttctgca cgggttaatg aggaactttg ccctcccagc caggcgatcc
     1201 gctgatgccc attgcgaatg agatgctccg tcaacaactg tgcagcctgc atgttgtccg
     1261 ggcgaaccgt atcaacatca tcaagataac tggcacggga agcgaaaatc accgggatag
     1321 ctttttcttc tgccatccgc cgcaggtcat cgctgcttcc tgcagccccg gcaattacca
     1381 caccatcgac accctgattc agtaacagtg aaaaccgctg tgccagctgc tcgccgtctt
     1441 taccgccgtg aagcaaaaaa accatccgtc cctgtgcttc cagagcttcc gtcaatccgg
     1501 ccgtcaattc ggcgtaaaac ggcgcagata aatcacggac gatcaaacca atgacgccgc
     1561 tttgcccgcc gcgcagcgcc gacgcctggc gattgcgcac aaatcccagc tcttcaatgg
     1621 cggcgttaac gcgttctcct gtggcggtag agattcgccc tttgccatta agcaccagcg
     1681 aaacggtact taccgacacg cccgcagcca gcgcaacatc atgaatggtt atttttttgg
     1741 cggtagccat gcagatgacc tactccctga ttatgtgaca gataaaacgt tttacctttt
     1801 atttatctta tacccgctat tatcgttgcg taatgtgatt tatgactcac taaaatttga
     1861 taaaacgttt tatcttctcg cgcaatttac tgaatccaga ttgttctcta cgaggagtcg
     1921 ttttatgacg gcgaaaacag caccgaaagt cacgctgtgg gagttcttcc agcagttagg
     1981 caaaaccttc atgttacccg tggcattatt gtcgttctgc ggcattatgc tcggcattgg
     2041 tagttctctt agcagccatg atgtcataac cctgatcccg gtcctgggca accccgtgtt
     2101 gcaggctatc tttacctgga tgagtaagat tggctcgttt gcttttagtt tcctgcctgt
     2161 catgttctgt atcgccatcc cgctgggcct ggcacgcgaa aataaaggcg tagcggcatt
     2221 cgctggcttc gtcggttatg cggtaatgaa cctcgcggta aacttctggt tgaccaataa
     2281 aggcattctg ccaaccacag atgccgccgt tctgaaagcc aataacatcc agagcattct
     2341 tgggatccag tcgatcgata ccgggatcct cggtgcggtg atcgccggta ttatcgtctg
     2401 gatgctgcat gagcgtttcc ataatatccg cctgccggat gcgctggcat tcttcggcgg
     2461 tacgcgcttc gtaccaatta tctcctcgct ggtgatgggc cttgtcggcc tggtgattcc
     2521 attagtctgg ccgattttcg ccatgggtat tagcggcttg ggccatatga taaacagcgc
     2581 gggtgatttc ggaccgatgc tgtttggtac cggtgaacgt ctgctgttgc cgtttggtct
     2641 gcatcacatt ctggtggcat taattcgctt taccgacgca ggcggcacgc aggaggtctg
     2701 cggtcaaacc gtcagcggcg cactgaccat cttccaggcg caattgagtt gcccgaccac
     2761 tcacggtttt tctgaaagcg tcacgcgttt cctttcgcaa ggtaaaatgc ctgcgtttct
     2821 cggcggtcta ccaggtgcag cgttagctat gtatcactgc gcgcgcccgg aaaatcgcca
     2881 taaaattaaa ggtctgctga tttctggcct gatcgcctgc gtcgttggcg gcactaccga
     2941 accgctggaa ttcctgttcc tgttcgtagc gccagttctg tatgtcatcc acgcgctgtt
     3001 aaccggcctc ggcttcaccg tcatgtctgt gctcggcgtc accatcggta ataccgacgg
     3061 caatatcatc gacttcgtgg tgttcggtat tttgcatggt ctgtcaacca agtggtacat
     3121 ggtgccagtg gtggcggcaa tctggtttgt cgtttactac gtcatcttcc gtttcgctat
     3181 cacccgcttc aatctgaaaa ccccggggcg cgatagcgaa gttgccagct caatcgaaaa
     3241 agccgttgcc ggtgcgccgg gtaaatcagg ttacaacgtt cctgcaatcc tcgaagcatt
     3301 aggcggtgcc gacaatattg tcagcctcga taactgcatt acccgtctgc gtttgtctgt
     3361 ggaagatatg tcgcttgtta atgtgcaggc actgaaggac aatcgggcaa ttggcgtagt
     3421 acaacttaat caacataacc tgcaggttgt tatcgggcca caagttcagt cagtaaaaga
     3481 tgaaatggcc ggtctgatgc atactgtcca ggcataagga taagatatgt tcgatttttc
     3541 aaaggtcgtg gatcgtcatg gcacatggtg tacacagtgg gattatgtcg ctgaccgttt
     3601 cggcactgct gacctgttac cgttcacgat ttcagacatg gattttgcca ctgccccctg
     3661 cattatcgag gcgctgaatc agcgcctgat gcacggcgta tttggctaca gccgctggaa
     3721 aaacgatgag tttctcgcgg ctattgccca ctggttttcc acccagcatt acaccgcaat
     3781 tgatacgcag acggtggtgt atggcccttc tgtcatctat atggtttcag aactgattcg
     3841 tcagtggtct gaaacaggtg aaggcgtggt gatccacacg cccgcctatg acgcatttta
     3901 caaggccatt gaaggtaacc agcgcacagt aatgcccgtt gctttagaga agcaggctga
     3961 cggctggttt tgcgatatgg gcaagctgga agccgttttg gcgaaaccag aatgtaaaat
     4021 tatgctcctg tgtaacccac agaatcctac cgggaaagtg tggacgtgcg atgagctgga
     4081 gatcatggct gacctgtgcg agcgtcatga tgtgcgggtt atttccgatg aaatccatat
     4141 ggatatggtc tggggcgagc agccgcatat tccctggagt aatgtggcgc ggggagactg
     4201 ggcgttgctg acatcgggat cgaaaagttt caatattccc gcgctgaccg gtgcttacgg
     4261 gattatagaa aatagcagca gccgtgatgc ctatttatcg gcactgaaag gacgtgatgg
     4321 gctttcttcc ccttcggtac tggcgttaac tgcccatatc gccgcctatc agcaaggtgc
     4381 accgtggctg gatgccttac gcgtctatct gaaagataac ctgacgtata tctcagataa
     4441 aatgaacgcc gcgtttcctg aactcaactg gcagatcccg caatccactt atctggcctg
     4501 gcttgatcta cgtccgttga atattgacga caacgcgttg caaaaagcgc ttatcgagca
     4561 agaaaaagtc gcgatcatgc cggggtatac ctatggtgaa gaaggtcgtg gttttgtccg
     4621 tctcaatgcc ggctgcccac gttcgaaact ggaaaaaggt gtggctggat taataaacgc
     4681 catccgcgct gttcgctaac cccaattgcg caacgtaaaa aatcgttgcg caatcgtgga
     4741 tttttaccct gctttgtttt tataatggtg cgcactttta tatccagaaa aagagtgcga
     4801 ccatgattga taccaccctg ccattaactg atatccatcg ccaccttgat ggcaacattc
     4861 gtccccagac cattcttgaa cttggccgcc agtataatat ctcgcttcct gcacaatccc
     4921 ttgaaacact gattccccac gttcaggtca ttgccaacga acccgatctg gtgagctttc
     4981 tgaccaaact tgactggggc gttaaagttc tcgcctctct tgatgcctgc cgccgcgtgg
     5041 catttgaaaa cattgaagat gcagcccgta acggcctgcg ctatgtcgag ctgcgttttt
     5101 caccaggcta catggcaatg gcacataagc tgcctgtagc gggtgttgtc gaagcggtga
     5161 tcgatggcgt acgtgaaggt tgccgcacct ttggtgtgca ggcgaagctt atcggcatta
     5221 tgagccgaac cttcggcgaa gccgcctgtc agcaagagct ggaggccttt ttagcccacc
     5281 gtgaccagat taccgcactt gatttagctg gtgatgaact tggattcccg ggaagtctgt
     5341 tcctttctca cttcaaccgc gcgcgtgatg cgggctggca tattaccgtc catgcaggcg
     5401 aagctgccgg gccggaaagc atctggcagg cgattcgtga actgggtgcg gagcgtattg
     5461 gacatggcgt aaaagccatt gaagatcggg cgctgatgga ttttctcgcc gagcaacaaa
     5521 ttggtattga atcctgtctg acctccaata ttcagaccag caccgtagca gagctggctg
     5581 cacatccgct gaaaacgttc cttgagcatg gcattcgtgc cagcattaac actgacgatc
     5641 ccggcgtaca gggagtggat atcattcacg aatataccgt tgccgcgcca gctgctgggt
     5701 tatcccgtga gcaaatccgc caggcacaga ttaatggtct ggaaatggct ttcctcagcg
     5761 cagaggaaaa acgcgcactg cgagaaaaag tcgccgcgaa gtaacaaaaa tggatggtgc
     5821 aaattgcacc atccattttt cacgcaaggc acaacgtcgc gcgatgtttg gcggattcga
     5881 tgcccagctc aatcaactcc attacctgga ttgcctggct tgccggaacc ggattttcac
     5941 catcgccatt taacgcatca cgaatcgccg catagtaagc cggataattc ccaggcaccg
     6001 tcaacagcgt ttcttcgaca cgttcctcac cttccacgcg ggtaagtacg ccatcacgca
     6061 tatcgtagcc ccagtcttcc tgcggcagac gctcgccatt tttcagacgt tcttcctgtg
     6121 gatcgaggcc atatttcaca taactgcctc gggatccatg cacgatatac cgtgctgact
     6181 cagcggctgc cagcatggta ccgtgtaaaa tgactcgccg ctgtggatag gacaagatgg
     6241 cgtggaaata atcggtcgac tgcgctccgg gccgtaactg tgccaaatct accgtcatgc
     6301 tgaccggtaa accaaatagc gtaatggcct gatcaagaag atgcggtgct aaatcgtacc
     6361 agataccgct gcctgggccg ccctgttcac gccaacgatc gcgcacctgc ggacggaagc
     6421 ggtcaaaatg agactcaaag taagcaactt cacccagcac gccttccgcg agcaaacctt
     6481 ttagcgtcaa gaaatcgcta tcccaacggc ggttatggaa tacagacagc acacgcccca
     6541 ggctttttgc cagcgcatcc agctctcgcg cttgtgacag tgtcacggta aagggtttat
     6601 caacgaccac atgtttaccc gcctcaagcg ccgctttggc taacgggaaa tgggtatcgt
     6661 tgggtgtagg aatgacaatc aggtctatgt tgggatcgtt aaacagatgc ttcggctcag
     6721 agacaaccgt aaccgttggc cagtcggctt ttacttttgt ttcatcactg ctggagatta
     6781 ccgccagttc cagcccgggc gtgcccgcaa tcaggggcgc atggaaggtt ttgctcgcat
     6841 aaccataccc aatcaaccca acacggatgt tgtcgctcat gtcattgcct ctcattgaag
     6901 tatgatggct atttgacact atcctttacc cacgctcaac agtttaataa cctgccagca
     6961 ataagggatg ttgtttaact taagtcaaaa aaatagcgaa ttttccaacg acaaaagcta
     7021 aatatcgcaa aaacctcagt aaaaatcttg ctggagctat tattgctaag taacatttac
     7081 cccctgaagt taatggatca atcaagagag atgtgggctg taatgaatcg tcttattgaa
     7141 ttaacaggtt ggatcgttct tgtcgtttca gtcattcttc ttggcgtggc gagtcacatt
     7201 gacaactatc agccacctga acagagtgct tcggtacaac acaagtaagc tctgcacttg
     7261 tggagcgaca tgctgcccgt ccgggtgcat gttttcactt gtcggatatt aaaccaggaa
     7321 tttattatct tgttcgatgt tgttggtgat tgtcagggat agtaaagcgc gttactcttt
     7381 tcagagcagt ttttcatccc tgaatatttg ccatcagcgc attcttatta ttgtaataac
     7441 acaagcgttc acttttggtg aaattttatc atcactaata atacctaccc ctttggtatt
     7501 aattatttta tggacctttt atgactgttc aggactactt attaaaattt cgcaaaatca
     7561 gttcactcga aagtctggaa aaactctacg accatcttaa ttacaccctg acggacgatc
     7621 aggaactgat caatatgtat cgtgctgccg atcaccgtcg cgcagagctg gtttctggcg
     7681 ggcgtttgtt tgacctcggc caggtaccga agtccgtctg gcactatgtc caataaagaa
     7741 agtagcgatc ggttataaaa gccttataat aacgccccta aaattagatt tgcctgtttg
     7801 cgtcgctatt ctggagaacc tatgaccacc acaacgccac aacgtattgg aggctggttg
     7861 cttggcccgt tggcctggct tttagtcgct ctgttgagta cgacgctggc gttgttgctg
     7921 tataccgctg cgttatcttc tccgcaaaca tttcaaacgc tcggcggaca agcactgacg
     7981 acacaaatct tatggggcgt ttcttttatt accgctatcg ccatgtggta ttacacactg
     8041 tggttgacca tcgctttctt taaacgtcgg cgttgtgttc ctaaacacta tattatctgg
     8101 ctgcttattt ccgtattgct ggcagtaaaa gcattcgctt tttcacccgt ggaagttggc
     8161 atagccgttc gccagttgct gtttactttg ttggcaaccg cattgatcgt cccctatttc
     8221 aaacgctcgt cgcgggtaaa agcgacgttt gtgaatccgt aataacctta cagttaacct
     8281 gttgtcgcct gctctggatt aacggataat aggcggcttt tttatttcag gccgaaaaat
     8341 gactgactac ctgttactgt ttgtcggaac tgtactggtc aataactttg tactggtcaa
     8401 gtttctcggt ctctgtccgt ttatgggggt ttccaaaaaa ctggaaaccg cgatgggcat
     8461 ggggctggca acaacgtttg tgatgacgct gacgtctatt tgcgcctggc ttatcgatac
     8521 gtggattttg atcccactta atctgattta cctgcgcacc ctggcattta ttctggtgat
     8581 tgctgtggtc gtgcagttca ccgagatggt ggtgcgcaaa accagcccgg tgctttaccg
     8641 tctgctgggg atttttttgc cgcttatcac caccaactgt gcagtgctcg gcgtggcgtt
     8701 gctgaatatc aatctcgggc acaatttctt gcagtcggcg ctgtacggtt tttccgccgc
     8761 tgtcggtttc tcgctggtga tggtgctctt cgccgccatc cgcgaacgcc ttgctgtggc
     8821 tgatgtcccg gcaccttttc gcggtaatgc cattgcgtta attaccgcag gtcttatgtc
     8881 tctggccttt atgggcttta gtggtttggt gaagttgtaa tgaatgctat ctggattgcc
     8941 gttgccgccg tgagcctgct gggcctggcg tttggcgcca ttctgggtta tgcctcccgc
     9001 cgttttgcgg tggaagacga tccggtcgtt gagaaaattg acgaaatctt accgcagagc
     9061 cagtgtggtc agtgcggtta tcccggctgt cgcccctacg cggaagccat cagctgtaac
     9121 ggtgaaaaaa tcaaccgttg cgccccaggt ggcgaagctg tgatgctaaa aattgccgag
     9181 ttgcttaatg tcgagccgca gccgctggat ggcgaagcgc aagagctaac gcctgcgcgg
     9241 atggtggcgg ttattgatga aaataactgt attggctgca ctaaatgtat tcaggcttgt
     9301 ccggtagacg ccatcgttgg cgctacccgt gtcatgcata cggtaatgag tgatctctgt
     9361 acgggctgca atttatgtgt tgatccgtgc ccgacgcact gcatctcgtt gcaaccggtc
     9421 gcagaaacac ctgactcctg gaaatgggat ctgaacacca ttcccgtgcg tatcattccc
     9481 gtggaacacc atgcttaagt tattctctgc attcagaaaa aataaaatct gggatttcaa
     9541 cggcggcatc catccaccgg agatgaaaac ccagaccaac ggtacccccc tgcgccaggt
     9601 actactggcg cagcattttg ttattccact gaaacagcat attggcgctg aaggtgagtt
     9661 gtgcgttagc gtcggcgata aagtattgcg cggccagccg cttacccgtg gtcgcggcaa
     9721 aatgctgcct gttcacgcgc ccacctcggg taccgttacg gctattgcgc cccactctac
     9781 ggctcatcct tcagctttag ctgaattaag cgtgattatt gatgccgatg gtgaagactg
     9841 ctggatcccg cgcgacggct gggtcgatta tcgctctcgc agtcgcgaag agttaatcga
     9901 gcgcatacat cagtttggtg ttgccgggct gggcggtgcc ggattcccga caggcgttaa
     9961 attgcagggt ggtggagata agattgaaac gttgattatc aacgctgctg agtgcgagcc
    10021 gtacattacc gccgatgacc gtttgatgca ggattgcgcg gctcaggtcg tagagggtat
    10081 tcgcattctt gcgcatattc tgcagccacg cgaaattctt atcggcattg aagataacaa
    10141 accgcaggcg atttccatgc tgcgcgcggt gctggcggac tctaacgata tttctctgcg
    10201 ggtgattcca accaaatatc cttctggcgg tgctaaacaa ttaacctaca ttctgaccgg
    10261 gaagcaggtt ccacatggcg ggcgttcatc cgatatcggc gtattaatgc aaaacgtcgg
    10321 cactgcttat gcagtgaaac gtgccgttat tgatggcgag ccgattaccg agcgtgttgt
    10381 aaccctgact ggcgaagcaa tcgctcgccc gggcaacgtc tgggcacggc tggggacgcc
    10441 agtgcgtcat ttattgaatg atgccggatt ctgcccctct gccgatcaaa tggtgattat
    10501 gggtggcccg ctaatgggct ttaccttgcc atggctggat gtcccggtcg taaagattac
    10561 caactgtctg ttggctccct ctgccaatga acttggcgaa ccacaggaag aacaaagctg
    10621 catccggtgt agcgcctgtg ctgacgcctg ccctgtggat cttttgccgc aacagttgta
    10681 ctggttcagc aaaggtcagc aacacgataa agctaccacg cataacattg ccgactgcat
    10741 tgaatgtggg gcttgcgcgt gggtttgccc gagcaatatt cccttggtgc aatatttccg
    10801 tcaggaaaaa gctgaaattg cggctattcg tcaggaagaa aagcgcgccg cagaagccaa
    10861 agcgcgtttc gaagcgcgcc aggctcgtct ggagcgcgaa aaagcgactc gccttgaacg
    10921 acataagagc gcagccgttc aacctgcagc caaagataaa gatgcgattg ctgccgctct
    10981 ggcgcgggtg aaagagaaac aggcccaggc tacacagcct attgtgatta aagcgggcga
    11041 acgcccggat aacagtgcaa ttattgcaga acgggaagcc cgtaaagcgc aagccagagc
    11101 gaaacaggca gaactggagc aaactaacga cgcagcaacc gttgctgaac cacgtaaaac
    11161 tgccgttgaa gcagctatcg cccgctccaa agcgcgcaag ctggaaca
//
LOCUS       DAJTSF010000117        11110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000117 DAJTSF010000000
VERSION     DAJTSF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.117"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO335_003394"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO335_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR6003649.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="OO335_003395"
     tRNA            complement(621..695)
                     /locus_tag="OO335_003395"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="OO335_003396"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="OO335_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="OO335_003397"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="OO335_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6003650.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3990)
                     /locus_tag="OO335_003398"
     CDS             complement(2932..3990)
                     /locus_tag="OO335_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003651.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO335_003399"
     CDS             complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO335_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6003652.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6068..6352
                     /locus_tag="OO335_003400"
     CDS             6068..6352
                     /locus_tag="OO335_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6003653.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6532..7842
                     /gene="fadI"
                     /locus_tag="OO335_003401"
     CDS             6532..7842
                     /gene="fadI"
                     /locus_tag="OO335_003401"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6003654.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7842..9986
                     /gene="fadJ"
                     /locus_tag="OO335_003402"
     CDS             7842..9986
                     /gene="fadJ"
                     /locus_tag="OO335_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6003655.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGVVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10189..10674
                     /gene="sixA"
                     /locus_tag="OO335_003403"
     CDS             10189..10674
                     /gene="sixA"
                     /locus_tag="OO335_003403"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6003656.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2778 a         2541 c         2747 g         3044 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagacttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtcagttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3961 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
     4021 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     4081 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     4141 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     4201 ttgtctgtgg ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     4261 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     4321 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     4381 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     4441 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4501 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4561 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4621 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4681 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4741 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4801 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4861 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4921 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4981 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     5041 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     5101 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     5161 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     5221 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     5281 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     5341 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     5401 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     5461 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5521 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5581 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5641 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5701 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5761 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5821 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5881 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5941 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     6001 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     6061 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     6121 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     6181 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtga aatccgaacc
     6241 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     6301 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     6361 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     6421 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6481 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6541 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6601 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6661 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6721 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6781 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6841 caagcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6901 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg
     6961 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     7021 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     7081 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     7141 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa
     7201 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     7261 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     7321 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     7381 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     7441 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7501 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7561 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7621 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7681 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7741 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7801 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7861 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7921 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7981 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     8041 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     8101 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     8161 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     8221 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     8281 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     8341 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     8401 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     8461 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8521 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8581 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8641 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8701 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8761 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8821 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8881 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8941 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     9001 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     9061 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     9121 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     9181 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     9241 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     9301 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     9361 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     9421 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9481 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9541 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9601 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9661 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9721 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9781 ttggtgtggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9841 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9901 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9961 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
    10021 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
    10081 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
    10141 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
    10201 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
    10261 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
    10321 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
    10381 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
    10441 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10501 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10561 ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt
    10621 acattcaact ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt
    10681 ccaaattatg cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat
    10741 ctgtcaacct cactactatc gaagactctc cttatctata aataaaacac tttctacatt
    10801 cattcggaaa ttattcttct acgcgtatct tgattttgcc caaagttaca tgttgaaatg
    10861 aataatatct gtttgacact cattgtcatt tattaataca gataatagag aaaatagagt
    10921 tgaataatca ggcaggatgt aattgaaact tgattaaatt ctggctatag tgtttatata
    10981 ttatattgtc aaaagtggat aaatgccaat cgcattatat gttcattgtt tgttttttaa
    11041 gctgttgata ttcattattg atatactttc tattttttct aaatacttat tccgtatttt
    11101 ttaaggatgt
//
LOCUS       DAJTSF010000118        11046 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000118 DAJTSF010000000
VERSION     DAJTSF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.118"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            409..1239
                     /locus_tag="OO335_003404"
     CDS             409..1239
                     /locus_tag="OO335_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6003657.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO335_003405"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO335_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6003658.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="OO335_003406"
     CDS             complement(2368..3828)
                     /locus_tag="OO335_003406"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6003659.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO335_003407"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO335_003407"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6003660.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="OO335_003408"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="OO335_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR6003661.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO335_003409"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO335_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR6003662.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO335_003410"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO335_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR6003663.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO335_003411"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO335_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR6003664.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..>11046)
                     /gene="fimD"
                     /locus_tag="OO335_003412"
     CDS             complement(9005..>11046)
                     /gene="fimD"
                     /locus_tag="OO335_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR6003665.1"
                     /translation="IGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKW
                     QHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVI
                     HGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFT
                     VPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLA
                     DRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQ
                     LVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTV
                     TQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRD
                     QMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSY
                     SVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANG
                     VTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTL
                     ADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSS
                     GIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2684 a         2827 c         2683 g         2852 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gagtgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgtgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgacgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc
     3901 ggtgggtatt aacggataaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt tgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtgagacatg tcaaccagaa ttctgaaatt ggttaaccgc gtcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaata gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtgga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatcc
//
LOCUS       DAJTSF010000119        10930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000119 DAJTSF010000000
VERSION     DAJTSF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.119"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..206
                     /locus_tag="OO335_003413"
     CDS             <1..206
                     /locus_tag="OO335_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003666.1"
                     /translation="FGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIA
                     IYLLAGCLISAMTALLMKDNQRS"
     gene            308..1762
                     /gene="amn"
                     /locus_tag="OO335_003414"
     CDS             308..1762
                     /gene="amn"
                     /locus_tag="OO335_003414"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6003667.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2105..2821
                     /locus_tag="OO335_003415"
     CDS             2105..2821
                     /locus_tag="OO335_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6003668.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3277..3352)
                     /locus_tag="OO335_003416"
     tRNA            complement(3277..3352)
                     /locus_tag="OO335_003416"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3317..3319),aa:Asn,seq:gtt)
     gene            complement(3453..5080)
                     /gene="yeeO"
                     /locus_tag="OO335_003417"
                     /pseudo
     CDS             complement(3453..5080)
                     /gene="yeeO"
                     /locus_tag="OO335_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5085..5160
                     /locus_tag="OO335_003418"
     tRNA            5085..5160
                     /locus_tag="OO335_003418"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5118..5120,aa:Asn,seq:gtt)
     gene            complement(5198..6148)
                     /gene="cbl"
                     /locus_tag="OO335_003419"
     CDS             complement(5198..6148)
                     /gene="cbl"
                     /locus_tag="OO335_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR6003669.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6250..7167)
                     /gene="nac"
                     /locus_tag="OO335_003420"
     CDS             complement(6250..7167)
                     /gene="nac"
                     /locus_tag="OO335_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR6003670.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7495..7570
                     /locus_tag="OO335_003421"
     tRNA            7495..7570
                     /locus_tag="OO335_003421"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7528..7530,aa:Asn,seq:gtt)
     gene            complement(7626..8561)
                     /gene="ldtA"
                     /locus_tag="OO335_003422"
     CDS             complement(7626..8561)
                     /gene="ldtA"
                     /locus_tag="OO335_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6003671.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8623..9702)
                     /gene="cobT"
                     /locus_tag="OO335_003423"
     CDS             complement(8623..9702)
                     /gene="cobT"
                     /locus_tag="OO335_003423"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6003672.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9714..10457)
                     /gene="cobS"
                     /locus_tag="OO335_003424"
     CDS             complement(9714..10457)
                     /gene="cobS"
                     /locus_tag="OO335_003424"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR6003673.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10454..>10930)
                     /gene="cobU"
                     /locus_tag="OO335_003425"
     CDS             complement(10454..>10930)
                     /gene="cobU"
                     /locus_tag="OO335_003425"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR6003674.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3037 a         2778 c         2367 g         2748 t
ORIGIN      
        1 tgtttggtgc cagttatcgc tacagtggtg ctggagtcgg ttatcaggtt gccagtgtgg
       61 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      121 ggcatagcat cgccatttat ttgctggctg gatgcctgat ttctgcaatg accgctttat
      181 tgatgaaaga caatcaacgc tcgtgatagc ctggcgaaga tcatccgatc ttcgccttat
      241 acttttgttt cacattcctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      301 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      361 cgcgctgtat gagcaatctg tagttgcttt acgcaacgcc attggcaaat atattacaat
      421 tggcgaatta cctgatgaaa acgcccgtaa acaaggtctt tttgtctatc catcactgac
      481 cgtaacctgg gacggtagca caacaaatcc ccccaaaacg cgcgcatttg gtcgttttac
      541 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatctgaa
      601 tgagcaacta acgttgcttt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      661 acatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      721 gagcgctgat ttaactcgct acttcccgac aacagaactg gcgcaaatcg gcgatgaaac
      781 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      841 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagtag aacattttca
      901 accgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
      961 cagccagatc ctcgatcctg atagtcccta cattgccctt tcttgtgctg gcgggaactg
     1021 gatcactgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1081 ccagatgcca gcatggcatt taattaccgc tgatggtcag ggtattactc tggtgaatat
     1141 tggcgtggga ccgtccaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1201 tatctggttg atgattggtc actgtggcgg attacgtgaa agccaggcca ttggagatta
     1261 tgtccttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1321 tatacctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcta ccaagttagt
     1381 cagcggcagg cctggtgagg aagtcaaaca gcggctgcgt acaggtactg tggtaaccac
     1441 agatgacagg aactgggagt tacgttactc agcttctgca cttcgtttta acttaagccg
     1501 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1561 gccatacggg acactactgt gtgtttcaga taaaccgtta catggtgaga ttaagcttcc
     1621 cggccaggct aaccgttttt atgaaggcgc tatttccgaa cacctgcaaa ttggcattcg
     1681 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcacgtaaat tacgaacctt
     1741 taatgagccg ccgttccgat aataagttat gaaccttctt atcagaaata gatgtgaagg
     1801 attaagtaag acatctggca aatagcctgc aataaacgcg ggctgcttgc ttccttgggc
     1861 agatgcgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     1921 aaacacaatt ataaaaaatc ccgccaacaa cattagtttt tataaaatta aatataagat
     1981 tatggcttta gaatattttt atttctaata gacgagattt ttcctgttat gatataatat
     2041 gccgaattaa cacatgttaa cgatttacca gtaatgtaaa taaattttcg aggagatcat
     2101 tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa acggctaaag acggtgcaac
     2161 gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct gctgccaaac aaggtgaacc
     2221 cgatccagaa ttaaacacat ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt
     2281 tccaaagcac gttattgata aagcaattga taaagccaaa ggcggcggag atgaaacgtt
     2341 cgtacaggga cgttatgaag gctttggtcc taatggctca atgattattg ctgagacatt
     2401 gacttcaaat gttaaccgta cgattgctaa cgttcgcaca attttcaata aaaaaggcgg
     2461 caatatcgga gcggcaggtt ctgtcagcta tatgtttgac aatacgggtg tgattgtatt
     2521 taaaggaaca gaccctgacc atatttttga aattttgctt gaagctgaag ttgatgttcg
     2581 tgatgtaact gaagaagaag gtaatattgt tatttatact gaacctgctg accttcataa
     2641 aggaatcgcg gcgctaaaag cagctggaat cactgagttc tcaacaacag aattagaaat
     2701 gattgctcaa tctgaagttg agctttcccc agaagattta gaaatctttg aagggcttgt
     2761 tgatgccctt gaagatgacg acgatgtaca aaaagtttat cataacgtcg caaatctcta
     2821 attatatttt aaagaaatct gtctttatgg cagatttctt taatctcata taattcttat
     2881 aaaaaatata atatttaact cgtcatattg cttataaaaa atccccgtcc ccagagaaat
     2941 aatatttatt aaaatggcag gtcttctttt tctgattacc gaagacaaag tggcaattac
     3001 gtatagtttc ccgataaaga cgcgacagct acgtcccgca taaggcattt ttctctttat
     3061 ctttgtacgg tacttcatgg aacagaattt ttgaccttgt gaatcgtgat atctgttggg
     3121 gaggggcaat ttgctcactg aagcgtgaga ctcgattaag cgcacgaaac acagaaatca
     3181 aaaaacccgg ccactttttt acaaagtggc cgggtaaaaa taatttttat tttttaactg
     3241 tcctgagtct catagagatg tctcaaaact aaaatttggc tcctctgact ggactcgaac
     3301 cagtgacata cggattaaca gtccgccgtt ctaccgactg aactacagag gaatcgtgag
     3361 aacgaggcga atattagcga tgcccaccca caatgtcaaa gcctgttttt taaatttgaa
     3421 atcgtttgct gaaataatct gcattttgtc gtttattccg acacaactgg ctttttttca
     3481 cacttttgcg gctcggatcg agggtatttc cacagccaac gtccagtaac cattcgccag
     3541 taaaacagca cggcccgcac agcccagtcg gcaaacattc ccatccagac accaaccaca
     3601 ccccagccaa gcatgattcc ctgcacataa ccgactacaa cccggcaacc ccacatgctc
     3661 aacatagaaa cccacatggc gtaacgggca tcacgagcac ccttaaatcc agcgggtagc
     3721 acccatgagg cggaccaaat aggcataaat aaagcattta gctaaatcag aatcacaacg
     3781 acgtgtttaa cctgtggatc ctgggtgtaa aacgatgcca taaccccggc aaagggagcc
     3841 gttagccagg cgatggccgt taatccaaga gtggacagcc agaacacatg ccgcaactga
     3901 atctctgctt gcgctatctg ccctaccccc aaccttcggc ctgtaatgat cgtagaagca
     3961 gaaccgagcg cacttccggg taagttgata agagccgcaa ttgagaacgc gataaaattt
     4021 ccggcaataa cgctggtccc catcccggca acgaacattt gggttaataa ccgaccactg
     4081 gtaaataaca ctgattcgac actcgctgga ataccaatcc ccatgacttc ccagataatg
     4141 ctaaaattca gcggtttaaa atagctcttt aacgaaatcc ttagcgcagg attaaaacca
     4201 atcgccagca cccacaaaat tgcaactgcg ccaatataac gagaaatggt taaacccaac
     4261 ccagccccga ccaggagaaa aggccgtaaa tcaatatgcc gctaataata atattaagaa
     4321 tattcaggct accgttaatc agtagcggta ttttcgtatt ccctgcacca cgaagtgccc
     4381 cgctaccaat aagagtgatg gcagcggctg gataactgag tacggtcagc tccagataag
     4441 tcaacgccag tgctttaact tctgtcgtgg catcacccgc gacgaaatca ataatttgtt
     4501 cgccaaaatg atgaattagc gttgccaaca gtacggcaaa caacgtcatg atcaccaatg
     4561 actgccgcgt cgccaccctc gctcgtcgtc gatcccgctt accgagacta aatgctacaa
     4621 cgacagtagt accaagatcg atagctgcaa aaaaagccat aatgaccata ttgaagctgt
     4681 ccgccaaccc cacgccggcc atcgcatctt ttcccagcca actgaccaga aaagtgctca
     4741 ggacccccat caacaggaca caggcgttct ccatgaagat aggaacagca agcggggtta
     4801 tctcgcgcta gaacaacact ttgtagctct tgcgtttaga gtgccagcga gtgccgtgaa
     4861 caacctggcg taaagcagag gagatattca aagccgacct taattgcaga aagtgaaacc
     4921 acatttcaaa tgatgaggga gaatcagcaa agctgcaaag attttcgcca acaaattgtc
     4981 tgcaaatgca acaaactgtt gatagaaacg gcaaacagtt gggaaattta aaaatcgggt
     5041 ttgacaaaat atttttcgcc gttaagatgt gcctcaacag cgattcctct gtagttcagt
     5101 cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca
     5161 aatttctgaa aagcccgctt ttatagcggg ctttttgcta tatctgataa tcaatttcct
     5221 cttcactgtt ttccatcacc tggcgcttga tatcctcaac tgacagtcct gcattacaaa
     5281 gttccagaaa gcgccaaaca tagttacgct gaagttgtcc tcgcttcagt cccaaccaga
     5341 cagtattagc atcgaaaaga tgccgcgtat ccaggcggat taaattctct tcctcttgtt
     5401 cgccgctgga ttgttcggca actaatccga tcccaagccc aagagcaaca taggttttaa
     5461 tgacatcaga atcctgtgca cttaatacaa tatctgccgg caaacctttg cgggtaaatg
     5521 cgtcatcagt gcgtgagcgc cccgtaattc cctgtcggta agtaattaac ggccacttcg
     5581 ctattgattc cagcgtcaat ggtgtaattt gcgttaaggg atgatcgagt ggaacaagca
     5641 aactatggtg ccaacaaaac catgggaagg cgacgagctg cgggtcatta ctcaaacgct
     5701 cgctggcgat accaatatca gcttcgccat tttgcaacaa cgtcgcaatt tcctgtggcg
     5761 aaccctggat tagctcgagc cgaacctccg ggaaaagttc gcgaaaagct ttaatgacct
     5821 ctggcaagct ataacgtgcc tgagtatgcg tcgttgcaat agtgagaaca ccagacgtat
     5881 cgttggtaaa caggtctgca agccgacgaa cattactggc ttcattcaga atacgttctg
     5941 caatgaccag taatgctttg cccggttcag tcatccccag cagtcgctta cctcgtcgaa
     6001 caaatatttc gatgccaaat tcatcctcca gttcccgaat atgacggcta acgcctgact
     6061 gtgaggtaaa aagcatattc gcaacctctg tcaggttgta atcctgacgt gcagcctcgc
     6121 ggattatctt tagttgttgg aaattcacgg taaactccgg gcagttcaga tatcccgtta
     6181 ttgttaaagt ctaatgcccg gcataacaaa taataaaaac ccgcatctta ttccatcccg
     6241 atataacgct tagctcacca attgccactg ccttttttcc atcactggag aactaatcac
     6301 tgacattaac aactctttca ctgcctgtgc ctgtggtgac aagtttgctc tggcgggtaa
     6361 atttaaggac aaagagagac tcattgaagg agtggtaatg cgtgacatcc acccatttac
     6421 tgcgccacat aacgaacgcg cagccgattc aggtaatact gcaacgccca tgccgctggc
     6481 aatcgctgcg gtaagcgtgg caatagactc aatttcacca atgacttttg ccgtgagccg
     6541 ccgtagggaa aaagcctcat caacacgaag tctaacagcg ctgtaatcac tggggagaaa
     6601 aaggttcatt tgcgcaatag cattaacatc aacgttttgc cccgggcaat cttgagttcc
     6661 taccaggaaa agatcttctt tcagcaaagc ctgactggat accccagcca caggggaatg
     6721 ctcataaatc accgccatat cgagttggtg atttatcagt ttttcgttaa gcactgcacc
     6781 actattttca tgaagataga taacgacctc cgggaattca gcgcgaaccg cctgtaataa
     6841 gggcatggtg atggatgacg cagcggttcc tggcgcaaag ccaatcgaga cttgccccga
     6901 taatgactga ccaacgttat gcaccgccag ttgggcctgc tcacactgac gtaaaatggc
     6961 ccgtgcatgg gtatagagaa tttttccggc gtctgttggc gtaacgcccc gttttgtgcg
     7021 aatcaaaagc tgttgattta actcaccttc cagtatggca acctgttggc tgagcgctgg
     7081 ttgtgcgata tgcaatactt cagcagcctg ggtcaggcta ccaatatcta caatttttac
     7141 gaagtatttc aggcgtctga agttcatgtt gcctccggtt tttaagaatc ggcccaagtg
     7201 ccgccattac ttacaaccag tatgcaagat gcctgccagt tctattttgg cgttgatgcg
     7261 caagctaacc aactgtcaaa taagagatta tgatagattc gtcattggcc ctttttatca
     7321 gcagtcgcat tcacctgccc tataaaagga gggtatgcac cacgatggtt cattacccaa
     7381 taagattgaa agctcaccac tttgttgaaa ttggcagcaa acaaacaaaa aaaatgcatt
     7441 tcaccctttg acatcaccat gcactgccat taatatgcgc cccgttcaca cgattcctct
     7501 gtagttcagt cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc
     7561 agaggagcca aattcaaaaa agcctgcttt cgagcaggct ttttactttc taattaccaa
     7621 cgctcttaaa acatctgtct tgaaccagaa ctaatttgca caggcattcc cgatcgatgt
     7681 tgcaacgcag catttgcgcg atttacatca acttcttgcc cgttgataaa cgcccgcaaa
     7741 gatggggtta ccggcaatgg cacttttcgg tcagactcat attctgcacg attgcgcgac
     7801 agcggctcat gaacttccag ccactttgaa ccatccggtt ccgttgtgta tttcactggc
     7861 tgatcaataa tttgtacacg agtgcctaca ggaacattat caaacagata tttgatatcg
     7921 tcattacgca gacgaataca gccctgactt acacggagcc caataccaaa attggcattg
     7981 gtaccgtgga tggcatacaa cctgccaata taaatcgcgt acagccccat ggggttatcg
     8041 ggccccgcag gaacaaatgc gggcaaactc tcccctcgtt tcgcatattc gcgccggatg
     8101 ttcggcgttg gcgtccaggt tggtgcttct tgtttacgtt caacggtagt cacccagtta
     8161 cgcggggttt ctcgcccagc ctggccgata ccaataggaa agacttccac tgtattactg
     8221 tctggcggat agtaataaag acgcatttca gcgacgttaa ccacaatccc tttacgaaca
     8281 gtggcgggca aaatcagttg ctgcggaatg gtgagttgtg agccaggctt cggcaaaaaa
     8341 acatcagcgc ccggattcgc ttccagcata ttacttaacc cttgcccgta ttgtgcggca
     8401 aaagtctcca gcggctgagt attgtgatca ggaacagtta cagtaagcga ctgccccact
     8461 aaacggctac cctctggagg taatggataa gttaccgcca ggctattttg gctggcaaaa
     8521 agcagagcaa atgagcaaag aatatttaca cgacgcatca tgtccctttc ctaagtcgcg
     8581 aatactatcc gataagtata gcttttatca gacttttcgt ttttagctgt tcaaatcaga
     8641 agtcgtattc cccggtagaa caatattact ggcagcaagt tcgcccatgt tgttgtatat
     8701 cgcacaagca gcttcgatga tggacatcgc cagagctgca ccactcccct cacctaaacg
     8761 catatccata ttgagataag gctccagccc caaatgcgag agcgctatac gcgcgccttt
     8821 ttctgccgac aagtgagaag gaatgagata cggtttgatt gcaggagaca tctggcaggc
     8881 tgcgagcgca gcagcataag aaagaaatcc atccagcaac acgggtaaac cgcaggaagc
     8941 agcacctaac atcactccag ctattccgac caaatcaaat ccacccactt ttgccaggac
     9001 atcaacacca tcctgaggat ttggttgatt caacgtaatc gcccgacgca caacatcaat
     9061 tttattagcc agtttatctg tcggcaggtt tgcgccaatc ccaaccactt cttcaggatc
     9121 ccggccagtg attgtgctga ctattgccgc tgccggtgtc gtgtttgcca tccccagttc
     9181 acctacacca aacagcgtga caccgttttt cgccagctcc cgcgtataac atatgacgtc
     9241 caaaagcaac ttttcagcct gacggcgact cattgccgga gctgaagcaa tattgccgct
     9301 acctcgtgcg acacgcatgt tgataagccc ggggataggc tcagcagtat caataccaac
     9361 atcaattacg tggacgttag cgcccgcttg tgctgccagc acacacacgc cggttgttcc
     9421 acgggtcata ttttcagcct gtatggctgt cacttctttt ggagaaatag cgaccccttc
     9481 ctcccagacg ccgtgatcgg cacacataac cagtaccgct tttttgccca catgcggtat
     9541 gccattcacc cccggcattc ctgccagttg tatggcaagc gcctccagct ttcctaggct
     9601 accaacaggt ttgaataacc cgtcaacatg ccgttgtgca cgcgacatag cggcagaatt
     9661 gatggctggg atcgtattca gtaaatcggc aattatttgc atgtcacgtt tctttatagc
     9721 agtgccagca ggaagaccag ttcaccaagc tctatggctg ctcccagcgt atcgcccgtt
     9781 tgcccgccca gcgtacgttt gagaagctga ccgaggatga aaatcgccac catcgtcacc
     9841 accatagcag ccacaccatg catacccagc aataatacgg cagcaaaaat agcggctaag
     9901 ccgagtgtga cgcaggtttg tcgcccatca attttgccaa tgaacacatt gccaagccct
     9961 tcttcccgcg cataacgatg acgatacatc aataatgcgg cagtcccgcg gctgaccgca
    10021 catgctgccg ccagcgatgc aaggatcggt tcgccacgca gagccaactc gcttaacacc
    10081 agaatctttg ccagtaccac aaaaatcaat gccagaccgc cgtgggtgcc taaacggcta
    10141 tcacgcatga tttccaacat gcgatcgcga ctacgtgcag aaaatacgcc atcacaggta
    10201 tcagcgaggc cgtccagatg gaattccccg gtcatcagcg ccagcacaag tacgctaaac
    10261 agtgccgcca gtggtgcgcc acaccatgcc tgcagcacca tgaagaccag cccgctaatc
    10321 gcgccaagca ataatccaat caaaggaaaa gtaataatac cgcgagaata atgttcgaaa
    10381 tccagtccct gggaccagcg acgcggtaca ggcagacgcg taataaatga gagcatcgcc
    10441 caaaataatt tgctcatttg atttttactc caatacccga aaccaccagc catacttcat
    10501 ttgccgcagc ggccaactgc tgatttaccc gcccggcaat atcacgaaaa tgtcgtgcca
    10561 gacgactctc cggcacaatc cccattccca cttcgttagt cactaataca acctttgcgg
    10621 ggcaacgttg gcaggcagca atcaacgact gaatctcagc attaatcgcc tgttccatcg
    10681 cctgataatc ccattcatca gggtctttat cgccgccata atcaaacaac agattagtca
    10741 ccattgtggt aacgcattcc agcaacacag cctcattcgg gttaatgtct gcatgaatta
    10801 attcatcaag atgttgccag cgctccactg tgcgccagtg ctccgggcgg ctttgccgat
    10861 gatgttctat ccgtgcagcc atctcatcat caaggatttg cgaggtagcg atatacagaa
    10921 cctgtgaaga
//
LOCUS       DAJTSF010000120        10866 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000120 DAJTSF010000000
VERSION     DAJTSF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.120"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO335_003426"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO335_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR6003675.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="OO335_003427"
     CDS             complement(1433..1759)
                     /locus_tag="OO335_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR6003676.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="OO335_003428"
     CDS             1894..2235
                     /locus_tag="OO335_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR6003677.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="OO335_003429"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="OO335_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="OO335_003430"
     CDS             complement(2833..3543)
                     /locus_tag="OO335_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR6003678.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="OO335_003431"
     CDS             3651..3956
                     /locus_tag="OO335_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR6003679.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4475
                     /locus_tag="OO335_003432"
     CDS             4155..4475
                     /locus_tag="OO335_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR6003680.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            5035..5613
                     /locus_tag="OO335_003433"
                     /pseudo
     CDS             5035..5613
                     /locus_tag="OO335_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6299..7288)
                     /locus_tag="OO335_003434"
     CDS             complement(6299..7288)
                     /locus_tag="OO335_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003681.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7373..7864)
                     /locus_tag="OO335_003435"
     CDS             complement(7373..7864)
                     /locus_tag="OO335_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003682.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(8185..8685)
                     /locus_tag="OO335_003436"
     CDS             complement(8185..8685)
                     /locus_tag="OO335_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003683.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8675..9190)
                     /locus_tag="OO335_003437"
     CDS             complement(8675..9190)
                     /locus_tag="OO335_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003684.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(9621..9989)
                     /locus_tag="OO335_003438"
     CDS             complement(9621..9989)
                     /locus_tag="OO335_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003685.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(10321..>10866)
                     /locus_tag="OO335_003439"
     CDS             complement(10321..>10866)
                     /locus_tag="OO335_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6003686.1"
                     /translation="YQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLADVLKTALL"
BASE COUNT         2776 a         2770 c         2685 g         2635 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc ccctatcttc tattgcacta ccgcactaga
     6001 caactagtac aaaacaaagg cccagaccat tggtccagac cgtagttagt taatcacagt
     6061 gacaagccac cacctgaagt cctgctgata ttcagtccag acttacctct gctcatggtc
     6121 ttacctccct cggtctcctg accttgggta tcgactacag cttgctgagc gtcatctgga
     6181 ggagctacag gaggagctac aggaggcggg accgaagccc caccttgagt cccaccacca
     6241 cctgtcaggc ccttggcgat ggacctgaag cctttactaa tgctcttacc cattggtctt
     6301 acgcttcggt cttggtgaac ttcaggcgtt tgactacagg caggacggca gtagctgagc
     6361 cagtgcccat cttgatggtc gtagcagctt cagcgacaac gaacacttta ccgaggattt
     6421 ccaagtcgtg ggactgagag tcgccatcac gtgaaaggac gaccgtaata ttctcaccgt
     6481 cgtgacggaa gcagccgaag ctcagggccg tgaagcgttt agccagcatg aatacaccct
     6541 tgtcttcacc gttgtaatag gcgatgtctg tccccagcat atcgacgata gcgtcttcac
     6601 cggagaagcc ttgcttagca gccagacggg acagtgcttt cagttggctc tcatgagcga
     6661 taataccgta gtcagacacc tcagaaccga aagaagactc tgtgttctca tacagagcgt
     6721 ccatgatagc gtctgcctta gcggtcaggc taccagtcag gacaggaagc tctacggtct
     6781 tcagcagctt agaggattcc agaacgtcga ccatgcgagc gttgatggta cggaccacat
     6841 tctcacagat gttcatagcg tcctgctgaa gtgtgccctg agtgaatccg gtggatgact
     6901 tggcgtccca gagcttggtc tgctcagaga tgagaatagc ctgctcgatt ggttcgacca
     6961 cgatttcttg tccagacgga acggtcacga cttcaggtcg cttgccacca cggacaggaa
     7021 cgaccacaga gcggtcagcc agagtgtgag caatgattgg ggtcggtaag ctgttacggg
     7081 tcggcaggac ttctacaggg aacgggatgt tctcttgagc gtctttaagt acaccgttca
     7141 gcatagcctt aaccgtctta gctaactgga gggcatagac ctgcgggtca gcgttagtcg
     7201 gcagaccttc attgacctga atgttgtcgg tatcggtgat gcgtactggc tcaatgccaa
     7261 actggaaagc gtctttaatc gtagtcataa taatcaatct cctaaattag tttaaatact
     7321 gtgagtcctc aaaactgagg agtccctaac atcgggtcag ggactgtgtg ttttacaagc
     7381 tgtccatcca gttaccgcct gactcaaggt cagcgacaat agcggcacgg gagcgaccag
     7441 cggaatgggt agcactgcgg aggctctcac cataggtccg gcgttcagct ttgacctgtt
     7501 cgcggcgctg tagctctttc tgccactctg catcggtcat gttctcttca cgcatacgct
     7561 cagcctgaag tcgttgctct ttagcgatac gctcagcttc agctcgtttg ttcatctggc
     7621 ggcgaccata ggcggtcatc aagtcattac tgaagaagtt gccaatggtc acgccagctt
     7681 cagccagagc ttccagtaac tccattgcct cgcggctggt agacggcagg cctgacaggt
     7741 agcccggaat gagggtcttg atagcgagcg acagcggata gggctgaccg tccagactca
     7801 cagtgttttc ataaaggttg atgtcggtga ctgacagctt ccagttgttg ttgttgaaca
     7861 tcataggtaa ttctcctctt gagagttgag actatcggta tcgcagacgc agtagcgcct
     7921 acgggtctcc ccgcatcggt catctcaaaa ggtttaaaat taggtttggt atggtcagga
     7981 ctgcttagca aaggctcggc gtaactgatt gtctaaatca cggtccacca gcatagctat
     8041 cagctcgtct cgactgtagt aagggcgctc agggtcttcg actccaagtc tcagcagaga
     8101 cagcagatga cgtgtgggca ggccacggta acgaagggtc gctttgccga taggtgtacg
     8161 agggtcaatc atagccatga ttacttacct ccactcacag cgaccagcgg tcacagcttg
     8221 atagtctgaa tactcagcct gtgagacttc acgtccggcg atgtagaggc ctgtgccatg
     8281 acgctggatg aattccttgg tctccggctt gatgtcctca gcagacgcta cagcagactg
     8341 cacggtggcg ctggaggtca tagacacgaa ggctcgggtt actgccagtc cgtacccaag
     8401 gtccaaccac cgaccgaact ctatagcggc tactgggtca gccagccacg aggtccctaa
     8461 gtgacccatg ttaatctttt taacaaaccc gttttctggg ttggttagac ttctaccagc
     8521 cttggtcctc atgaagcgag caacctcggt actacggtta tcttctacct taccaatctg
     8581 aatgcctacc cggcgtatct catccagtcg aaacaatccg tcctgactgg tgatgtcctg
     8641 accgaagata tgaatggtgt attcctgctt attgtcagaa gtcataagag gcacctcccg
     8701 ccagagcttc ggcaaaggac ttgaagcgct gttcttggtc agctttcgcc tgaaggcgtt
     8761 cggcatcagg tgggaccagc ttatcaaagg tgccgttttc gtaatcttca gttaggacct
     8821 gaaggcggcg ggaccatttg ctctccacct gcatcatcgc gttagagatg tgcttcaagt
     8881 cggcatcaga gcgtgacgct atccaatcca tctcttctga gtcaccctca caggtgtaag
     8941 gattgttgaa atagaagatg tcctgcaggc cacgcttcag ctcttcctcg gtgtgtgtct
     9001 ggaggtatga ctggataggc tccgcgtaca tctcccggat tttctcatag tcccagtctt
     9061 ccgcctgggc ataccatcgg gataacttca gcaggtctaa gttatagcga cagcaatcga
     9121 cactggactc tgcattggtc agcaggatga ttaagaactg gcggttagtt actggtaatt
     9181 cggtattcat acaaagatgc tcctttggta aagcactttc tgtctccaca tggagtccgc
     9241 accattcaga cgcatcctaa acggacccat cagaaagtgc ttaacgaaag tgaatggtta
     9301 aatgttgacg gtctggagtg accatgttct ccatgtacgt ataaaggtgc gaccgcatgt
     9361 caggacgagc tgtaaggact ccagatggtg agcggtgata gacttaagcc aatgtagtga
     9421 gtagacgagc tactaaccga gacgactaaa gaacgctaac gagctggagg tcgatacagg
     9481 aggtctgtca gaggcggtca cgggccttga ttacttagga gagatactta atgtcggttg
     9541 accagatgct cgcatgttct gacgcatcag actcagaggc ggaggctggt ctccctatag
     9601 tgatagttta gggtccgtaa tcatgaacca gctttactgg cgtggtactg gacggattta
     9661 ctggcgctga cgacggtagc atagacgtag cgagcgtagc gagttgaaac cataggttca
     9721 cccaaggcaa cacgcttacg gctcagggac tcctgcacag cgcttacagt taatgtgtcg
     9781 tgcgttagta gttgggtcag aatcaagtat gggctgacag gcttggtatt gcttgccatt
     9841 gattcgttca ggcgaccagc accagtaagg acatcatcca gccaaccact caggtcgccc
     9901 tgaccatcga atgacagcag gtccccgcag tgctgctgca agtaggcttt ggtgaacgtg
     9961 ttgtcgtgag ttgcgattgg tcgtgccata gagtatctcc tgtagttgaa ttagttggtt
    10021 cccctatagt gataatcaag tccatcactg gtgatgcata cagtagcgcg acaggatgtg
    10081 actcacaggt tgaaccgaat gtcaagagga gaccatagga atttggaggg actggagttt
    10141 aggacgcagt ttaaataaga ccacagacag acttagagtc ggacagccga aggcggacca
    10201 gtgggcctat agtcaggagg gattatgatt attatcaccc tccctaactt cagtcagact
    10261 tcagtgaccc aaggtctcaa ccatcaagcc gtatatggtc tccaccttgg gtctccctac
    10321 ttaaagtaaa gctgtcttaa gcacatcagc cagcctaccc acagcgtgtc ctttcccgta
    10381 caggtcatac gtaattgact gactgctgtg acctaagatg ccttgagcgt ccaccagatt
    10441 gactccacgc tctttcattc gggtcgccag ccaatgtctc agactgtgga gagagacatc
    10501 ctgagagtct cctagggcct cagtcaggac ccttgagtta aaccagctag agtaggcgct
    10561 cggagtcatc ccgaagagag gaccctcatc aggctgcatg tctacccacg acaggaagga
    10621 cgtcaggtca aacccataag cgccatcggt taacggcaca agtcggactg aatgtttgtt
    10681 cttcacagac ttcccgtccc catcttcatt gatgtcgata cagaccagtc cattgtccaa
    10741 ggttacaatg tcacgtttag tcaggtggca gacctcagca gaccgagcgc cagtgaccac
    10801 agacaccaga gacgcccatc tacgggcctc agaggtcgtg tgagccgtgt gtcgtgtgaa
    10861 ctgata
//
LOCUS       DAJTSF010000121        10799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000121 DAJTSF010000000
VERSION     DAJTSF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.121"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..270
                     /locus_tag="OO335_003440"
     CDS             <1..270
                     /locus_tag="OO335_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6003687.1"
                     /translation="LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIF
                     PALNDVPALSGLIDTLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            281..1021
                     /locus_tag="OO335_003441"
     CDS             281..1021
                     /locus_tag="OO335_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR6003688.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1080..1466
                     /locus_tag="OO335_003442"
     CDS             1080..1466
                     /locus_tag="OO335_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6003689.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1475..1804
                     /locus_tag="OO335_003443"
     CDS             1475..1804
                     /locus_tag="OO335_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6003690.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1776..4817
                     /locus_tag="OO335_003444"
     CDS             1776..4817
                     /locus_tag="OO335_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6003691.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4817..5146
                     /locus_tag="OO335_003445"
     CDS             4817..5146
                     /locus_tag="OO335_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6003692.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5146..5844
                     /locus_tag="OO335_003446"
     CDS             5146..5844
                     /locus_tag="OO335_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6003693.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5849..6592
                     /locus_tag="OO335_003447"
     CDS             5849..6592
                     /locus_tag="OO335_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6003694.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6529..7131
                     /locus_tag="OO335_003448"
     CDS             6529..7131
                     /locus_tag="OO335_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6003695.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7192..10671
                     /locus_tag="OO335_003449"
     CDS             7192..10671
                     /locus_tag="OO335_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6003696.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10739..>10799
                     /locus_tag="OO335_003450"
     CDS             10739..>10799
                     /locus_tag="OO335_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HCR6003697.1"
                     /translation="MRKLYAAILSAAICLAVSGA"
BASE COUNT         2547 a         2559 c         3454 g         2239 t
ORIGIN      
        1 ctgagtgatg ctcagtacac cggaaaaatg acggatgaag atgactggca ggctgttctg
       61 catattgccg tcttcatccg ggcacaggca ccggattcag agctggatat gtggatggag
      121 agcaccattt tcccggccct gaatgatgta ccggcacttt ccggactcat cgacaccctg
      181 aacccactcg gttttaacta tcaacgtgat aatgagatgg ctacctgggc gatggcggaa
      241 atcacgtacc agatcacgta cacgaattaa ggaggtggta atgaccacac caaatccact
      301 ggcaaagacg aaaggtgcgg ggacgacgtt ctggatgtat accggcaacg gcgatgcgtt
      361 tgcgaaccct ttgtcggaca ctgactggct gcgtcttgcg atggtgaagg atctgcaacc
      421 tggcgaaatg accgctgatg cagaagatga cacttatctc gatgatgaag atgcagactg
      481 gaaaacgaca acccaggggc agaaatccgt tggtgatact tcggcgacgc tggcctggcg
      541 tccgggtgac agcgggcaga aaaaactggt tcagttgttc gactccggtg aagtctgcgc
      601 gtttcgtatc aaatatccca acggcactgt tgatgttttc cgcggctggc tgagctcact
      661 gggtaaaacc attgcctcaa aagacgtgat gacccgcact gtgaaaatca gcggtgtggg
      721 gcgtccgtat ctggcagagg aaggcactga aaccgtgggt gttaccggac tgacggtggc
      781 accggcatct gtcagtgtca aagcgggagc aaccaccaca ctgaccttta cagtaaaacc
      841 tgacggggcc agtgacaaag cgatcagtgt gcattcgtca gatcctcaga ctgcttcggt
      901 gaccctgagc gggcttgtgg ccacggtgaa aggcgtgaag cagggcagtg tcagcatcgt
      961 gggcatgacc tctgacggag agtttgtggc agtggctgcg gtgaccgtca gcgcaccata
     1021 acaggacgat actcatcatt gccccggtta tccggggctt tttgcatccg gagaacatga
     1081 tgtttctgaa acaggatacg tttaattatg aaaaacagtc cgtggtgctc agtgagctgt
     1141 ccgggctgca gagaattgaa tatctgacgt ttgttcagca gcgaacggca aagtttgatg
     1201 cacaggaggg agaactgccg gaggctgaac gacagattgc ttttctgcgt atgggaatgg
     1261 atatcaatgc ctggctggtt tcccgctcac tgtggaatgc tgagcagtct caggatgttg
     1321 agacgctttg cgcatccatt atgacaacat ggtcgtatga tgcgctgggc gcgggggcgg
     1381 agagggttct gtcgctgagc ggtatgggga ccattgagaa tgccggggat gatgatcatg
     1441 aggcgctgac gccggaaaag tcctgacgcg ggaaatgcag tttgtcatgc ggcttgcccg
     1501 ggagttccgg cgggcagact ggcggcggat gctgtcggat atgtcggcca ctgagcttgg
     1561 tgagtggggc gattatttcc ggatgcagag cttcagtgat gtgtggatgg atgcgcagtt
     1621 tgcctcgctg aaggcattga tcgtgagaat ggtgtccggc agcagcgatg ctgcggtggc
     1681 tgatttcagc cttttaccgg aagagaacgg gataccggag cgaacggacg aagaactgat
     1741 gcatcttggg gaaggtattt ccggaggtgt gcgttatgga ccagatagcc aacctggtca
     1801 ttgatttggg gattgatgcg gcagagttta aaaatgaaat tccccgtatc aaaaaccttc
     1861 tgaatggtgc agccagcgat gcagaacggt cttctgcccg tatgcagcgt tttatggagc
     1921 gtcagactca ggcggcccgg cagacaatgc aggcggcttc ttcggctgca acagcagcat
     1981 cagcccatgc gcagacggtg gagaagaatg cacgggctca tgaacgcatg gcccgtgagg
     2041 tggaacaaac ccgtctgcgc gtggatgccc tgaatcagaa aatgcgcgag gaacaggcgc
     2101 aggccagggc actggcggag gcgcaggata aagcggctgc cgccttttat cgccagattg
     2161 acagtgtgaa acaggccggt gcggggcttc aggaattaca gcgtattcag cagcagatcc
     2221 gacaggccag aaacagtggc ggggttggtc agcaggatta tctggcgctg atttcggaga
     2281 tcacggcgaa aacccgtgcc ctgacgcagg cagaggaaca ggccacccgg cagaaagcgg
     2341 cgtttatccg ccagcttaaa gagcaggcaa cccgccagaa tctgtcgtct tctgagttgc
     2401 ttcgcgccag ggcggctcag ctgggggtaa gcagtgctgc agaagtgtat atccgcaaaa
     2461 tggagcgggc aggaaaagcc acacattcgc tggggctgaa aagtgcggca gcccggcggg
     2521 agctgggggt gttaatcagt cagatggcgc gaggcaattt tggtgcgctg aggggatccg
     2581 ggataacgct ggctaaccgt gccggatgga caggcgcact gatgtcgccg aaaggcatga
     2641 tgactggcgg cgttattggc ggacttgtcg cggcggtcct gggtctgggt aaagcctggc
     2701 atgacggccg gaaggagggc gagaaattta accgtcagct ggcgctgacg ggacattatg
     2761 ccggtgtcac tgtcgggcag ttgtggaaac tcagccgggc catatccggg aatggtatca
     2821 cgcaacatgc ggcagccggt gcgctggcac aggtggtggg cagtggggca tttcatggaa
     2881 acgatattgg tatggtggcg aaagctgccg cacagatgga gcgatcggtt ggccagtcgg
     2941 tcagcgatac cataaatcag tttaagcggc tgaaggatga tcctgtaaat gccgcgaagg
     3001 cgctggacaa tgcgctgcat tttttgaatg ccactcagct tgagcagata cgcgtccttg
     3061 gggagcaggg gcggtccagt gatgcggccc ggatcgccat gtctgcgctg gcagaggaaa
     3121 ccggtaaacg cacatctgat attgataata atctcaatgc gctgggtagt acgctgcaaa
     3181 ccttgtctga ctggtggaag cagttctggg atgcggccat gaatattggt cgtgaagact
     3241 cgctggatgc gcagattgat gcgttacagg aaaaaattca gcgcgcgaaa aaatatccgt
     3301 ggacaaacgc ctccacacag gtggagtacg atcagcagcg tcttaacgat cttcaggaga
     3361 aaaaacgccg gaaggatttg caggatgcaa aagcgcaggc agaacggaat taccaggagc
     3421 aacagaaacg ccggaatgct gaaaatgccg cgctgaaccg gatgaatgaa acggaagctg
     3481 cacgacatca gcgggaaatt gcgcgtatta atgccatgca gtacgccgac caggctgtca
     3541 gggatgcggc gatacagcgt gaaaatgaac gttacgaaaa agccattaag aaaaatacac
     3601 gggcaacccg taatgatgag gccacccggt tattgctgca gtacagtcag cagcaggcac
     3661 aggtggaagg gcagattgct gctgccagac agtcagcagg cattgccact gaaaggatga
     3721 ctgaagcgca taaacagctt ctggccctgc agcagcgcat cagcgacctg gacgggaaaa
     3781 agctgacggc agatgaaaag agtgtgctgg cccgtaagaa tgagctgatt caggcgctga
     3841 cgctgctgga tgtgaaacag caggagctgc agaaacagac agcgcttaac gacctgagaa
     3901 aaaaaacggt tcagctgacc agccagctgg cagacaaaga acgtgcactg cgtgagcagc
     3961 acaatctgga tattgccact gcaggtatgg gggataagca gcggcagcgc taccaggcac
     4021 agttgcgcat ccggcaggaa taccggcaac agttgcaaca gcttgagaat gacagtcgcc
     4081 agaaaggcac ttacgggacg gaggactacc ggagggctga ggaggtgctg aaggggagcc
     4141 tgaagcgaca actgaatgaa aacaaacgct actggcagga actggaagtg gcgcagggcg
     4201 actggaaaaa cggtgccatg cgggcgtttc agaattttac ggcggatgcg gataatgcgg
     4261 cgggaacggc agaacagatg tttacagtgg cattcagcag tgccggtaat gcactggcga
     4321 cattctgtac caccggtaag ctgaatttta aatccttcac ctcttccctg ttgtcagata
     4381 tggccagaat tatggcacag atggccatga tgcaggcggt aaagggcgtc ggttctttat
     4441 tcggcttcac gactaatgct gatggcggtg tttaccagtc tgctgatttg agtcgctaca
     4501 gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc aaaaggcgcg ggtgtgatgg
     4561 gggaagcggg acctgaagcc attctgccac tgcgtcgcgg tgctgatggt aagctggggg
     4621 ttgtggcgga tactggtggt tcaggcatgg tgatgtttgc cccgcagtac aacattgaga
     4681 tcaataacga cggcacgaac gggcagatag gtccggctgc cctgaaggtg gtttatgacc
     4741 tcgggaaaaa agcggccgcg gactttatgc aacagcaggc ccgtgatggt ggccggttaa
     4801 gtggagcata ccggtaatgg agacgtttca ctggaaagtg cgcccggata tgaatgtggt
     4861 atcagagccg aaagtggtga cagtgaagct gggcgatggt tatgaacagc gtcgtgcggc
     4921 gggactgaat aaccagttgt cgacttacag cgtgacgata cgtgttcgta aaggtgaaca
     4981 cccatcttta aaagcctttc tggaacggca cggtggcgtc cgcgcatttc agtggacgcc
     5041 accttatgac tggaaactga tcagggtggt ttgtcgtaaa tggtcggcaa gcgtgggggc
     5101 gttatgggtg actgtaacgg ccgattttga acaggtggtg aactgatgca ggatatccgg
     5161 caggaaacac tgaatgaatg cacccgtgcg gagcagtcag cccgggtgga gctctgggaa
     5221 atcgatctga cagaggtcgg tggtgagcgt tattttttct gtaatgagca gaacgaaaaa
     5281 ggtgagccgg tcacctggca ggggcggcag tatcaggcat accccattca ggggtcgggg
     5341 tttgaactga acggcagggg ctgtgctgcc cgtccgacac tgacggtctc taacctgcac
     5401 ggcatggtca ccgggatggc ggaagatctg cagagtctgg tcggcggaac ggtggtccgg
     5461 cgtaaggttt acgcccgttt tctggatgcg gtgaacttcg tcaacggaaa cagcgacgcc
     5521 gatccggaac aggaggtgat cagccgctgg cgcatcgagc agtgcagcga actgagcgcg
     5581 gtcagtgcct cttttgtgct ggccacacca acggagacgg atggcgcggt tttcccgggg
     5641 cgtatcatgc tggcgaatac ctgtatgtgg acttaccgtt ctgatgagtg tggttacacg
     5701 ggcagggcag tggctgacga gttcgacaaa ccaacaacgg atatccggaa ggacaaatgc
     5761 agcaagtgta tgcgcgggtg tgagttgcgc aacaataccg gtaatttcgg cggtttcctt
     5821 tccatcaata aactttctca gtaaatccat gacacagaca gaatcagcga ttctggcgca
     5881 cgcccggcga tgtgcgccag cggagtcgtg tggcttcgtg gtgagaacgc cggagggaga
     5941 caggtatctt cccagcgaga atatctccgg tgagccggag gaacggttcc ggatggctcc
     6001 ggaggactgg ctgcgggcac aaatgcaggg tgagattgtg gcactggtcc acagtcatcc
     6061 cggtggtctg ccctggctga gtgaggctga ccggcggctg caggtgcaga gtgatttgcc
     6121 gtggtggctg gtctgccggg ggacgattca caagttccgc tgtgtgccgc atctcaccgg
     6181 gcggcgcttt gagcacgggg tgacggactg ttacacgctg ttccgggatg cttatcatct
     6241 ggcggggatt gagatgccgg attttcatcg cggggatgac tggtggcgtc acggtcagaa
     6301 tctctatctg gataatctgg aggccacagg gctgtatcag gtgccgttgt catcagcaca
     6361 accgggcgat gtgctgctgt gctgttttgg ttcatcggtg ccgaatcatg ccgccattta
     6421 ctgcggcgat ggtgagctgc tgcaccatat tcctgaacaa ctgagtaaac gagagaggta
     6481 taccgacaaa tggcagcgac gcacacactc cctctggcgt caccgggaat ggcacgcatc
     6541 tgcctttacg gggatttgca acgatttggc cgccgcatcg accttcgtgt gaaaacgggg
     6601 gctgaagcca tccgcgcact ggccacacag ctcccggcgt ttcgtcagaa actgagtgac
     6661 ggctggtatc aggtgcgcat tgccgggcgt gatgcaggtg aaaccgaatt gtctgcccgt
     6721 cttaatgagc cgctggcaaa tggtgcagtg atccacatag taccgcgtct ggtgggagct
     6781 aaaagtggcg gtgtgtttca ggcggtgctg ggggcagctg ttatggcggt tgctatatgg
     6841 atgccggggg taggaattat ggcgagtaat ctgctgtttt ctctcggtgc cagtatgacg
     6901 cttggcggtg ttgcacagat gctggcaccg aaagccagaa ctccccgtac acagacaacg
     6961 gataacggca aacagaacac ctgtttctcc tcactggata acatggttgc ccagggcaat
     7021 gttctgcctg ttctgtacgg tgaaatgcgc gtggggtcgc gggtggtatc tcaggagatc
     7081 agcacggcag acgaagggga tggtggtcag gttgtggtga ttggtcgctg atgcaaaatg
     7141 tttcatgtga aaccgcctgc gggcggtttt gtcgtttatg gagcgtgagg aatgggtaaa
     7201 ggcagcagta aggggcatac cccgcgcgaa gcgaaggaca acctgaaatc cacgcagttg
     7261 ctgagtgtga ttgatgccat cagcgaaggg ccggttgacg gtccggtgga tggattaaaa
     7321 agcgtgctgc tgaacggtac gccggtcctg gatagcgagg ggaagacaaa cttttccggt
     7381 gttacggtgg tgttccgcgc cggcgagcag gagcagacac cgccggaggg gtttgaatct
     7441 tccggctcag agactgtgct gggtacggaa gtgaaatacg acacgccgat cacccggacc
     7501 atcacgtcgg caaacattga tcgtctgcgc ctgaccttcg gtgtgcaggc actggtggaa
     7561 accacctcaa agggggaccg gaatccgtcg gaagtccgcc tgctggttca gatacagcgt
     7621 aacggtggct gggtgacgga aaaagacatc accattaagg gcaaaaccac ttcgcagtat
     7681 ctggcctcgg tggtggtggg taacctgccg ccgcgcccgt ttaatatccg gatgcgcagg
     7741 atgacaccgg acagcaccac agaccagctg cagaacaaaa cgctctggtc gtcgtacacc
     7801 gaaatcatcg atgtgaaaca gtgctacccg aacacggcgc tggtcggcgt gcaggtggat
     7861 tcagagcagt tcggtaacca gcaggtgagt cgcaattatc atcttcgcgg gcgcattctg
     7921 caggtgccgt cgaactataa cccgcagacg cggcaataca gcggtatctg ggacggaacg
     7981 cttaagccag catacagcaa caacatggcc tggtgtctgt gggacatgct cactcatccg
     8041 cgctacggca tggggaaacg tcttggtgcg gcagatgtgg acaaatgggc gctgtatgtc
     8101 atcggccagt actgcgacca gtcagtgccg gacggatttg gcggcacgga gccgcgcatc
     8161 acctgtaacg cttacctgac cacacagcgt aaggcgtggg atgttctcag tgatttctgc
     8221 tcggcgatgc gctgtatgcc ggtatggaac gggcagacgc tgacgttcgt gcaggaccga
     8281 ccgtcggata aagtgtggac ctataaccgc agtaatgtgg tgatgccgga tgatggcgcg
     8341 ccgttccgct acagcttcag cgccctgaag gaccgccata atgtcgttga ggtgaactgg
     8401 attgatccgg acaacggtca tgagacggcg acagagcttg tggaggacac gcaggccatt
     8461 gtccgttacg gtcgtaacgt cacgaagatg gatgcctttg gctgtaccag ccggggacag
     8521 gcgcaccgcg ccgggctgtg gctgattaaa acggaactgc tggaaacgca gaccgtggac
     8581 ttcagcgtcg gcgcagaagg gcttcgccat gtaccgggtg atgtcattga aatctgcgat
     8641 gatgattatg ccggtatcag caccggtggt cgcgtactgg cggtgaacag ccagacccgg
     8701 acgctgacgc tcgaccgtga aatcacgctg ccgtcctccg gtaccacgct gataagcctg
     8761 gttgacggaa atggcaatcc ggtcagcgtg gaggttcagt ccgtcaccga cggcgtgaag
     8821 gtgaaagtga gccgggttcc tgacggcgtt gcagaataca gcgtgtgggg gctgaagctg
     8881 ccgacgctgc gccagcgcct gttccgctgc gtgagtatcc gtgagaacga cgacggcacg
     8941 tatgccatca ccgccgtgca gcatgtgccg gaaaaagagg ccatcgtgga taacggggca
     9001 cactttgacg gtgaccggcg cggcacggtg aatggtgtca cgccgccagc ggtgcagcat
     9061 ctgaccgccg aagtcaccgc agacagcggg gaatatcagg tgctggcgcg atgggacacg
     9121 ccgaaggtgg tgaagggtgt gagcttcctg cttcgcctga ccgtggcagc ggacgacggc
     9181 agtgagcggc tggtcagcac agccaggacg acggaaacca cataccgctt caggcagctg
     9241 gcgctgggga attacagtct gacagtccgg gcggtaaatg cccgggggca gcagggcgat
     9301 ccggcgtcgg tatcgttccg gattgcggca ccggcagcgc ctgtcactat tgaactgata
     9361 ccggggtatt ttcagataac ggtggtcccg aaactggctg tatatgaccc gacggtgcag
     9421 tttgagttct ggttttcgga aaagcggatt gcggatatca ggcaggttga aaccagcgcc
     9481 cgctatcttg gcacggcgct gtactggata gccgccagta tcaatatcag gccgggccat
     9541 gattattatt tttacgttcg cagtgtgaac accgttggca aatcggcatt tgtggaggcc
     9601 gtcggtcggg cgagcgatga tgcggaaggt tatctgagtt tttataaagg gttgatcaat
     9661 aaaacgcatc tcggcaagga gttgtggacg cagattgata acggtcagct tgcgccggac
     9721 ctgactgaaa tcaggacgtc cattacgaat gtcagcaatg aaatcacgca aaccgtcaat
     9781 aaaaaactgg aaaatcagag tgctgcaatc cagcagatac agaaagttca ggttgataca
     9841 aataataacc tgaacagcat gtgggctgtg aagctgcaac agatgaagga cggacgcctt
     9901 tatattgcgg gtatcggtgc cggtattgag aatacgccag caggaatgca gagtcaggtg
     9961 ctgctggcgg cagacaggat tgcgatgatt aatcctgcga atggcaacac aaagccgatg
    10021 tttgttggtc agggcgatca gatattcatg aacgacgtgt tcctgaaacg cctgacggct
    10081 ccgaccatta ccagcggcgg taatcctccg gcattttccc tgacacctgg cggacggctg
    10141 acggcgaaaa atgccgatat cagcggtaac gtgaacgcga actccgggac gctcaacaac
    10201 gtcacgatta ataagaactg tcgggttctg ggaaaattgt ccgcgaacca gattgaaggc
    10261 gatctcgtta aaacagtggg caaacctttc ccacgggact cccgggcacc ggagaggtgg
    10321 ccatcaggga ccattaccgt cagggtttat gacgatcagc cgtttgatcg gcaaattgtt
    10381 attcccgcgg tggcgtttcg cggtgctaaa catgagcgga agaataacaa tatttattcg
    10441 tcatgccgcc tgatagtgaa gaaaaacggt gctgaaattt ataaccgaac gaccctggat
    10501 aatacgctga tatatacggg tgttattgat atgcctgccg gtcacggtca catgacgctg
    10561 gagttttctg tatcggcatg gctggtaaat ggctggtatc ccacagcaag tatcagcgat
    10621 ttgctggttg ttgtgatgaa gaaatccaca gcaggtatca gtatcagctg aattttataa
    10681 cccagatacg ggcaccagaa atggtgcctt ttttattgca gaaaagcgag aggtaattat
    10741 gcgtaaactt tatgctgcca ttttgtccgc agccatctgt ctggctgtat ccggtgcgc
//
LOCUS       DAJTSF010000122        10463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000122 DAJTSF010000000
VERSION     DAJTSF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.122"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            2..202
                     /gene="cspG"
                     /locus_tag="OO335_003451"
     CDS             2..202
                     /gene="cspG"
                     /locus_tag="OO335_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR6003698.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            213..401
                     /locus_tag="OO335_003452"
     CDS             213..401
                     /locus_tag="OO335_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6003699.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            376..606
                     /gene="ymcE"
                     /locus_tag="OO335_003453"
     CDS             376..606
                     /gene="ymcE"
                     /locus_tag="OO335_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR6003700.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            596..769
                     /locus_tag="OO335_003454"
     CDS             596..769
                     /locus_tag="OO335_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR6003701.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(818..1891)
                     /locus_tag="OO335_003455"
     CDS             complement(818..1891)
                     /locus_tag="OO335_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6003702.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1993..4704)
                     /gene="torS"
                     /locus_tag="OO335_003456"
                     /pseudo
     CDS             complement(1993..4704)
                     /gene="torS"
                     /locus_tag="OO335_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4787..5815
                     /gene="torT"
                     /locus_tag="OO335_003457"
     CDS             4787..5815
                     /gene="torT"
                     /locus_tag="OO335_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR6003703.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5788..6480)
                     /gene="torR"
                     /locus_tag="OO335_003458"
     CDS             complement(5788..6480)
                     /gene="torR"
                     /locus_tag="OO335_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR6003704.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6567..7739
                     /gene="torC"
                     /locus_tag="OO335_003459"
     CDS             6567..7739
                     /gene="torC"
                     /locus_tag="OO335_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR6003705.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7739..10279
                     /gene="torA"
                     /locus_tag="OO335_003460"
                     /pseudo
     CDS             7739..10279
                     /gene="torA"
                     /locus_tag="OO335_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10276..>10463
                     /gene="torD"
                     /locus_tag="OO335_003461"
     CDS             10276..>10463
                     /gene="torD"
                     /locus_tag="OO335_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6003706.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2523 a         2685 c         2788 g         2467 t
ORIGIN      
        1 aatgactggt ttagtaaaat ggtttaacgc agataaaggt tttggcttta tcactcctga
       61 tgatggcagc aaagacgttt tcgtccattt caccgccatc cagagcaatg aattccgcac
      121 gctgaacgaa aatcagaaag ttgaattttc tattgagcag gggcaacgtg gccccgcggc
      181 agcgaacgtt gttacgctct aaggttgccg ttattactca acatctccat ttccgctgtc
      241 catgttgtca tggttcacag taccgtacat cggcattcga tgtgacggag cgaaaccctt
      301 tgggcgctaa gtgtattttt tgtaaatcga ctatgatcac ctttgataac gtcgcgctgc
      361 aaatacgcac tgaccatgcg ccgctggatt tcacaaaata atatcaggct ccctcgtgga
      421 gcctttttta tatctgcctt atttttcttc aacgctgtat gtatagtaag cgataacctg
      481 ttgattattg aatctttcgg gaagatggct tataacattt cttacctgac cagggtaccg
      541 ggaaccaaca ccttactggc gtgttgctgt ctttcaagac cagacgaggt taacagtgaa
      601 tattgaagag ttaaaaaaac aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat
      661 cgccgagctg aacaagaata cagggaaaga agtctctgaa attcgcttca tcgcacgaga
      721 aaaaatgacc gggcttgaaa gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca
      781 tcaccccgtg cgaggtgatg ttccgcttgt tgctaattta gtgaccaatc attggcgctt
      841 gtggaattaa gcgtcggtac aattcctccg gcaccgggct ttgccatact cccgcataca
      901 ttgcgtaacc aatcaccgca aacataatcc ccagaaccag tagcgtcatt aaccagccag
      961 acaacgcaaa ggctttttta tttgctgcag gtttttgcag tgaaaaggtc aatgttgagg
     1021 ctaccggaca tgactctacg caagtcatac agccggtaca ttccactgtt cgtacctgaa
     1081 ttaatttatc taccgggatc cgtgatgggc acttttttgc gcatttgcca caatcgatac
     1141 aactttcggc attgcgacga atcttaaacg gcgacaatag cgaaaccacg cccatcagcg
     1201 cgccatatgg gcaaagataa cgacaccagg catggcgaat aaacaggctg gcaatcagca
     1261 aaacggtcac gctgattaat gtcgcggtcc ccatatgacg aaagaaatcg agcattttaa
     1321 cgtccatcac cacgctatag ggcgacaaca taaaatagtg aatcgcctga gcgggcatca
     1381 ataacgcgat atagagaaat aaactcaaca gcaaatactt cacgccgcgc agaggaatat
     1441 ccagccagcg gggaaggaca cattgccgac caaacagttt gttaccgaga tcgccgatta
     1501 attcagaaag cgtaccaacc gggcataacc atgagcaaaa ggcctttttg agtaatagac
     1561 tgatgacgat aaaagcgacc aataacagca tcgcggcggc gtggacggac ggtaactgac
     1621 ctgttgcaag gctatatttc agattcatca gaccggcaat cggtagccag ccttcgatac
     1681 ctcccggtct ggcgacaaat gtcgtactac ctgccgtttc gtaatagcgc acccaatacc
     1741 agaacgtgat ggcaatataa atattcattg ccaatagtaa gaattgcgtc gctttacgcc
     1801 aggtcgtggc attgcgccag tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc
     1861 gctgccagcg ggttcttttt ttctctgcca tgattttgcc agtccgttaa gttgcatacc
     1921 aaatgccact attctagtta ttcttaactg gctgatattg attcaaatcg cgttcaggtc
     1981 tttcttattc aatcatgctt ccagagcggc aacactgcgt gtaatttctt cgtgtggaag
     2041 gggggcagat aatggctgct gctccagttg tgcgcatagc tgactggcgc tacgcattcc
     2101 cagactcgag caactgcttt ttagctgatg cgcggcacgc tttattttct cgctgtcctg
     2161 gctggcgcgg gcaatgtcga tttcatcgag aagcggcagg gcatgttgtt taaataatgc
     2221 cagccattcg tggatcttct ccgtccccat taactgagcg tcttcattga gttgcgagac
     2281 atccagtggt agatcgttat tggcttgcag ttggagatag tgcgccagta actgaccgag
     2341 cacttcacgc ggcaccggtt tagggataat cccgcgaaat agtgaactgg tacgctggcg
     2401 cagcgtttcg tcaatgacat gggcgctaaa gccaatcaaa accagcgacg gatattgctg
     2461 tgccagttgc cgggcaaggg taatgccgtc gatatccggc agatcaaaat ccaccagtgc
     2521 ggcagcaaac ggttcgctat tttgcagtgt ctctaaagcc tgcgcggcat tgccaatagc
     2581 aacaacctgc gcaccactgg tgttcagcat ctcaacggta attcgctggg ttagcgggtt
     2641 atcttcaatt aacagcaaac gtaaaccgtc aagacgcacc gcctgattgg ctgtttttgg
     2701 cacgggtgca gtggcaacac gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg
     2761 cgtgctggtg gcgctcagtt cgccgcccat cgcctgggcc agacggctac tgatagtcag
     2821 ccccagcccg gtgccgccgc gtttgccgct tacctgcaca aatggctgga agatttctgc
     2881 cagtttcgcg ggatcaatac cgcagccgct gtcttccact tcgaccagcc attgctcgcc
     2941 atcagtgcga ctacgcagga taatgtgccc ttcgtcagta aaacgcaggg cattgctcaa
     3001 taggttggta atgacctgac gaatacgtcg tggatcgccc attaacgcgc tcggtacatc
     3061 atcggcaatt gccgttgcca ggcgaatcgg gcgacctttc acccgtccgc tcattaattg
     3121 cagggtactt tccagcagcg ggcgcggttc aaagggctca tcgctgactg aaacattttt
     3181 gccacctgct tcgatagccg aataatcgag aatatcgttg aggatggtca gcaacgattc
     3241 gccagagtcg gtaatcgcct gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc
     3301 cagcagttga gcagtgccga gaataccgta cagcggtgtg cggatctcat ggctcatcgc
     3361 cgtcagaaac gccgattttg cctggctggc tttttctgct tccgcccgtg cctgtcggtg
     3421 ttctatcacc agttcctgca attcagctgt acgcgctttg acctgcgccg ccagctgttc
     3481 gcggtggcga ttcagtgcat gaacactgct gcgaaacgca tccatcagcc gcccgatggt
     3541 atccagctcc cgtacgccag cggtttccgg gaaaggggag tcaatatcac cgtccagcag
     3601 ccgttgcagc gcctgcgttt gttcggcaag tggacgcgtg aatgagcgat aaaccacgcg
     3661 ccagaggatc ataatcagtg cgcaaagtga aaccatcccc agcaataaca ggctgtattg
     3721 cccgcgtgca ctggcttttt ccagatgtgc cagtccatgc tgattgtgca gctcaatggt
     3781 gtcgaccagc tgactgactt cgctactaaa ctgcgcgaac tgggcgatat tattttgggc
     3841 gagagtttgc aggtgattgc tgatttcact gtcctgctgg aacagcgcca acagatcgct
     3901 atattggcta acggtagtta acgttgttgc gacctgcgca cgaactcccg gatcttcaat
     3961 gcgtatttgc cgacgttgca gaattttcac cgcattattg agctgctttt ccagcgttgg
     4021 tgcatttttc tggatctgct ccagccccag attcatcacc atttgctgca cccgcagagc
     4081 gctaaggcgc agttcattca tctggttaac atactcaaga tcgatatcaa tcagccgatc
     4141 gagtgcactt tcagcagcct gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg
     4201 ggtcgctcca gcggaagttg tcgcattatt cgcctgacct tgctccaggc gtgcgatctc
     4261 atcggcggca gctatctgct gactgagttg ccgttgttgc tggcgtagtt gcagacgccg
     4321 ccccaccagt tccccttgct gacgtaatga acgggagatc tcctgctcct gttgttcaat
     4381 agcggtggta tcaaaacctt gttcccgtaa cgcttgcagc aacgcattaa tcttcaggct
     4441 ttatgcggtg agcattcgcc cctgcgcctg ccacatcttt tcgttatcgg cactggtcag
     4501 gttctgcgcg gcgaaaagtt cccaggcgct ggcttcgctc aactggcgcg ccatattcat
     4561 ggtaggaatc aatgcctgag tattgtcttt ttccacctgg ctgataaagc gcaggttgta
     4621 ccatcccacc agggtactgg tcagggttaa cagcgccatc agggcaaagc ccatccagag
     4681 tcttcgggtc agggttaaat tcacggtcgg tgcacttaag gtgaaaaagg ttgagtcgca
     4741 aagtggaatg catctagcat aaagccttat tattgatgag gctatcatgc gcgtactgct
     4801 atttttactt ctttcccttt tcatgttgtc ggcattttcg gctgataacc tgttgcgctg
     4861 gcatgatgcg cagcatttca cggtgcaagc ctctatgccg cttaaagcca aacgcgtatg
     4921 gaaactgtgc gcgctttatc ccagcctgaa agattcatac tggttatcgt tgaactatgg
     4981 tatgcaggag gctgctcgcc gctacggtgt ggatttaaaa gtgctggagg caggcggcta
     5041 cagccagttg gctacccagc aagcacaaat cgaccagtgt aaacagtggg gcgcagaggc
     5101 cattttgctc ggtagtagca cgacgtcatt tcccgacctg caaaagcagg tagcaaatct
     5161 gccggtgatc gaactggtaa atgctcttga tgatccccag gtgaaaagcc gcgttggtgt
     5221 accctggttt cagatgggct atcaacctgg gcgatatttg gtgcaatggg cgcacggtaa
     5281 accactgaac gtgctgctga tgcccggacc cgataacgcc gggggcagta aggagatggt
     5341 cgagggtttt cgcgcagcca ttgccggaag cccggtgcgt attgttgata ttgcgcttgg
     5401 tgataacgat attgaaatcc agcgcaacct gttgcaggag atgctggaac gccacccaga
     5461 aatcgacgtc gttgccggaa cggccattgc ggcagaggcg gcaatgggtg aagggcgtaa
     5521 cctgaaaacg ccgcttaccg tggtgtcgtt ttatctttca catcaggtgt atcgcgggct
     5581 gaagcgggga agagtgatta tggctgccag cgatcaaatg gtctggcagg gggaactggc
     5641 agttgagcag gccatcaggc aattacaggg gcaatcggtg tctgataatg tcagcccacc
     5701 gattttagtt ctgacgccga aaaatgccga ccgagaacat atccgccgct cgctgtcacc
     5761 ggggggattt cgtccggtct atttttatca gcacacatca gcggctaaga aataaccttc
     5821 accatgttgc gtcaccagta aatccgcgct gagtttatga cgtaaacgac gaattaacac
     5881 atcgacggtg cgcaggtcag ggttttccac ccgacgcgca gaaagcatac gcagcagacg
     5941 ctcacggctg agaatttcgc ccggattagt cacaaatgcc accaacattt catactctgc
     6001 gcgggtcagt ttaatcggct cgccatcccg ctccagcgta tggcgcgaca cattcaggca
     6061 ataacccgca aagcgatagc agtagtcctg agtgtgcggt tgagcttgtc gcgcgaggtc
     6121 gattcgccag agcagatttt tcacccgtac taccagttcg cgcagttcca gcggtttggt
     6181 gacgtaatcg tctgcgccca tttccagccc aacaatacgg tcaatccgat cgctgcgtcc
     6241 ggtaaccaga ataatcccca ccgttgagcg ttctcgcagg gcgcgggtta acatcaggcc
     6301 attttcatcg ggtaagttga tatccagcag aattaaatct accggctgat tctgcataat
     6361 ttcccgtagc ccggcaccgc tcgccgtaac ggaaacggta tacccctcct gagtgaagta
     6421 ggattgtaat cgcgcctggg taaccggctt atcttcaaca ataacaatgt gatgtggcat
     6481 cagagggttt tactcattct gttcatatct gttcatattt gctcattaag atcgcttcac
     6541 taaaccataa ttctacaggg gttattatgc ggaaactctg gaacgcgcta cgccgaccca
     6601 gtgctcgttg gtcggtactg gcgctggtcg caattgggat tgtgattggc attgcgctga
     6661 ttgtattgcc acacgttggg atcaaagtca ccagcacaac cgaattttgt gtcagttgcc
     6721 acagtatgca accggtgtat gaagaatata aacagtcggt gcatttccag aacgcctccg
     6781 gcgtgcgagc tgaatgccat gactgtcata tcccgccgga tattccaggc atggtgaagc
     6841 gcaaactgga agcgagcaac gacatttacc agacctttat tgcccactcc attgatacac
     6901 ctgaaaaatt cgaagccaaa cgcgcggaac ttgccgagcg tgaatgggcg cgaatgaaag
     6961 aaaacaactc ggcaacctgt cgctcctgcc ataactacga tgcgatggat catgccaggc
     7021 agcatcctga agcggcacgt cagatgaagg tggcagcgaa agataatcaa tcctgcatcg
     7081 actgtcataa aggtattgcc caccagttac cggatatgag tagcggcttc cgtaagcagt
     7141 tcgatgagct gcgcgccagt gctaatgaca gtggtgacac gctgtactct attgatatta
     7201 agccaattta tgcggcgaaa ggcgataaag aagcctctgg ttctctgctg cctgcttcgg
     7261 aagtgaaagt ccttaaacgt gacggcgact ggctgcaaat tgaaattacc ggctggacgg
     7321 aaagcgccgg acgtcagcgt gtactcaccc aattcccagg taaacgcatc tttgttgcct
     7381 cgattcgtgg tgatgtgcag cagcaggtga aaacgctgga gaaaaccacc gttgccgaca
     7441 ccaataccga gtggagcaag ttgcaggcca ccgcgtggat gaagaaaggc gacatggtga
     7501 acgatatcaa accgatctgg gcctatgcgg attcgttgta caacggcacc tgtaacgagt
     7561 gccacggcgc accggaaatc gcccactttg acgctaacgg ttggatcggc acgctcaacg
     7621 gcatgattgg ctttaccagt ctcgataaac gtgaagaacg cactttgttg aaatatctgc
     7681 aaatgaatgc gtctgacacc gcaggtaagg ctcacggcga taagaaggaa gaaaaataat
     7741 gaacaataac gatctctttc aggcatcacg tcggcgtttt ctggcacaac tcggcggctt
     7801 aaccgtcgcc gggatgctgg ggccgtcatt gttaacgccg cgccgtgcaa ctgcggcgca
     7861 agcggcgact gaggctgtca tctcgaaaga gggcattctt accgggtcgc actggggggc
     7921 tatccgcgcg atggtgaagg atggtcgctt tgtggcggca aaaccgttcg aactggataa
     7981 atatccgtcg aaaatgatcg ccggattgcc ggatcacgta cacaacgcgg cacgtattcg
     8041 ttatccgatg gtacgcgtgg actggctgcg taagcgccat ctgagcgaca cctcccagcg
     8101 cggtgataac cgttttgtgc gcgtgagctg ggatgaagcc ctcgacatgt tctatgaaga
     8161 actggaacgc gtacagaaaa ctcacgggcc gagtgccttg ctgaccgcca gtggttggca
     8221 atcgacgggg atgttccata acgcttcggg gatgctggcg aaagctattg ccttgcatgg
     8281 taatagcgtt ggtacgggcg gagattactc taccggtgct gcgcaggtga tcctgccgcg
     8341 cgtagtcggt tcaatggaag tgtatgaaca gcaaacctcc tggccgctgg tattgcagaa
     8401 cagcaaaacc attgtgctgt ggggctccga tttgctgaaa aaccagcaag cgaactggtg
     8461 gtgcccggat cacgatgttt atgaatatta cgagcagcta aaagcgaaag tcgccgccgg
     8521 tgaagttgag gtcatcagca tcgatccggt tgtcacatcc acccatgagt atctggggcg
     8581 cgagcatgtg aagcacattg cggttaaccc gcaaactgac gtgccgctgc aactggcgct
     8641 ggcacatacg ctgtacagtg aaaacctgta cgacaaaaac ttcctcgcta actactgtgt
     8701 gggttttgag cagttcctgc cgtatctgct gggtgagaaa gacggtcagc cgaaagatgc
     8761 cgcatgggct gaaaaactga ccggcattga tgccgaaacc attcgtgggc tggcgcggca
     8821 gatggcggcg aacagaacgc aaattattgc tggctggtgc gtgcagcgta tgcagcacgg
     8881 tgaacagtgg gcgtggatga ttgtggttct ggcggcgatg ctggggcaaa ttggcctgcc
     8941 aggtggtggt tttggctggc actacaacgg cgcaggcacg ccggggcgta aaggcgttat
     9001 tctgagtggt ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gtgactacaa
     9061 aggctacagc agcactattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa
     9121 agtgatcaac tggaacggta aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc
     9181 cggaactaac ccattccatc gccatcagca gatcaaccgc attattgaag gctggcgcaa
     9241 gctggaaacg gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat
     9301 cgtgctgcct gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc
     9361 caaccgtggc attatcgcca tgaaacaggt ggtgccgccg tagttcgagg cgcgcaacga
     9421 ctttgatatt ttccgcgagc tgtgccgtcg ctttaatcgt gaagaagcct ttaccgaagg
     9481 gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg
     9541 acgcggcgtt catctgccag cgtttgatga cttctggaat aacaaagagt acgtcgagtt
     9601 tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc
     9661 gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgccg atatgaacta
     9721 cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc
     9781 cggctcgcag aagtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc
     9841 gcagctatgt gagtcggaaa cgctgcgtca gcaatatacg gtagcgggta aagagccagt
     9901 gttcattaac ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt
     9961 ctttaacgct cgcggtcagg tgttggcagg agcagtagtt tctgaccgct atgcacccgg
    10021 cgtggcgcga attcacgaag gggcatggta cgatccagat aaaggcggcg agcctggtgc
    10081 gctgtgcaaa tacggtaacc ccaacgtgct gaccatcgac atcgggactt cgcagctggc
    10141 gcaggcgacc agtgcgcaca ctacgctggt ggaaattgag aagtacaatg gaacagtgga
    10201 gcaggtgacg gcgtttaacg gccccgtgga gatggtggcg cagtgcgaat atgttcccgc
    10261 gtcgcaggtg aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg
    10321 ctggcgcagt tgttctcccg tgaactggac gatgaacaac tgacgcaaat cgccagtgcg
    10381 cagatggctg aatggttttc gttgctgaaa agcgaaccgc cgctcactgc ggcggtgaat
    10441 gatctggaaa acagtattgc cac
//
LOCUS       DAJTSF010000123        10370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000123 DAJTSF010000000
VERSION     DAJTSF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.123"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..270)
                     /locus_tag="OO335_003462"
     CDS             complement(<1..270)
                     /locus_tag="OO335_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6003707.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(364..926)
                     /locus_tag="OO335_003463"
                     /pseudo
     CDS             complement(364..926)
                     /locus_tag="OO335_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1130..1336)
                     /locus_tag="OO335_003464"
     CDS             complement(1130..1336)
                     /locus_tag="OO335_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR6003708.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1443..2006)
                     /locus_tag="OO335_003465"
     CDS             complement(1443..2006)
                     /locus_tag="OO335_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6003709.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3381..5216)
                     /locus_tag="OO335_003466"
     CDS             complement(3381..5216)
                     /locus_tag="OO335_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003710.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5317..5604
                     /locus_tag="OO335_003467"
     CDS             5317..5604
                     /locus_tag="OO335_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6003711.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5576..7105
                     /locus_tag="OO335_003468"
     CDS             5576..7105
                     /locus_tag="OO335_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR6003712.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7275..8492)
                     /locus_tag="OO335_003469"
     CDS             complement(7275..8492)
                     /locus_tag="OO335_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6003713.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8859..9845)
                     /locus_tag="OO335_003470"
     CDS             complement(8859..9845)
                     /locus_tag="OO335_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003714.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9842..10370)
                     /locus_tag="OO335_003471"
                     /pseudo
     CDS             complement(9842..10370)
                     /locus_tag="OO335_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3231 a         2371 c         1949 g         2819 t
ORIGIN      
        1 acaccagaca tcgttattaa gtagcagcac cataagataa ggagtggcat cgttagccac
       61 agcttcccta ctccagaggt aatataaagg ggtgggctcg actgatttat ctttacgtcg
      121 cttacactgc aaatgttcag aaattagtct atgcagctca ccagtaaaat cagcaatcag
      181 agaaggaatt gccttattca taccagggca aggtattaat ttaaactgta ataatttaat
      241 tttaggatgt gtagcagcag cccgatacag agttgcaagg acacactttt gccagagggc
      301 gttacaggaa agcttaatgt ttgattctgt atacataata aatcacctta tagttacaat
      361 aggtcaaaaa ccgctgtagc caaagttacg ctggcctgat gctttagtgt cgagcttcgt
      421 caaataatcc agacgtacca ataagcgctg gtactgctct gggaaattag gatcgttaat
      481 atccaggatg taacgtccat tagcagggaa atgaactaat ccctggtgat cttccagttc
      541 caaccctaag gcactgtacc aggctccggt aattaaccct ctcagggtgt cagagcgctc
      601 atagtttccc agatggtaat aggcgtcttt gttaaaaaaa ggcagacgtg ataatgacat
      661 ttaaaggact gcgtatattc tttcgcccag ataatacaaa ccgacgttgc gtaggtgcgt
      721 ttgccttgtt gttttttacg gtgttgatct gcattaattt tcgcattcag cgagttacgg
      781 aaacgagata tctctccggg gatcaggtta gggaaacagg taatgatatc cccgttgtca
      841 atcacaggag ggtaatggag atccccccgt attgctgtta ttctggaagt gtttttaata
      901 ttcgtattga tagttctcag agatatatca cactctagat attttaatta agtatcagtt
      961 cgatatcggc atattgtaca ctacctcagg tagatgatga gatcagatac agggatcaac
     1021 tgacggtgag cgcagtcccc acattttatc cagggcttac tgcatattcc caacagccat
     1081 tcgttagcca ggtagacgaa taccctgatt tgccgctggt tttgctttcc tatcgaaggg
     1141 cccatacatc atcacgccca cgaaacaacc gacgaaataa cgcaactttc tcatccgtgg
     1201 ataatacgaa aacgcactgc acagggctct gcggtttacg tcgccattca atcccatgag
     1261 cttcaagtaa tgagaccagc cgggtatttt ctgcccgcag tactgctagt tcatcgctgt
     1321 cagatttata aaccatatgc gttatcactt gtacctgtct cccggcggca ccacgactga
     1381 aattcgtgtg aaggcaaatc cacgcgataa cttacgatag ttcggacata agcccccata
     1441 attcagttat gtcgtggtcg accccggtta cttttttcgg gaagcctgct acggcccaga
     1501 ataataccgt gttctttctg atcctgttta gccagatagc taagggcgta acgtaagtcg
     1561 tctaccgcag atttatcact atagtgaatc acgtgatcga tacgcaccgg gtatttgtct
     1621 ttagcccggc acagatgaaa ataaccctcc ccttccgtga tccgcctcca gatatctccc
     1681 agttgcctgg atatctgata agagttcttg tgacgttgtc catccaggta gccgataaaa
     1741 tgaatatgaa gccctttgtc tggtgtatat tccattaccc agtaatatcc cgccaacatc
     1801 gtctgggttt cgctaagtaa acggtatatt tccatacaca tactgtgctt acaggaatgc
     1861 ccgaaactgg gcgtgtcttt cctgtaagca aaatcaattc tgaaaggtaa cagtttagaa
     1921 aaacgttgaa acataccatc catgtgttca ttcacgtctt tcagaatcat gaaatccatt
     1981 ttgtaattgg gattggcatt atacatttta tagtccttac tttataaaac tcacaggaag
     2041 agagaattac gtaaatactg agagtagaga aacgccgtac cacggcatta ataaacgaag
     2101 taaaccagca gcacagacaa cctgataaaa ctgttttgta ttaccgcatt actgtgatga
     2161 ggtaatactt cagaatacag tgagactctc ttaaactgag aaccagtacg aattacaatg
     2221 gaaaaacaca cctcatagaa ttgagtttca ggaagcacca ctaaattctt tacaggaagt
     2281 aatctgacat tcacaaaact cagtatcggc tagctacagg caacatcgct attaatattg
     2341 tcgaaccatt aagtaaagcc gagagagacc acgggtattt aatcattagc ataaaagaat
     2401 aatggtatac agaacatacc cacacaccat aagaagattg ttccatacag aaaagatcat
     2461 atttacctat acctataaac tgtaacggag tcataacacg ctctaatagt gttccgatac
     2521 gtaaccagtg ttaagtacgt acaggacaac attacacaca taattcacca tacagcttcc
     2581 cccatatgct tcactgcgta gtgtatggag atatatgcat tacacacata cagaatactt
     2641 agcataaaga ggagtgacat taccacgtaa agagataagt tatacaacaa acatacaaac
     2701 aatacggcac agacacacaa taccataact ccaactaata atactaatta accttaatta
     2761 tttatggtat atgaaataaa tgtgattctc ccggaactaa ctaacctaac cagacagatg
     2821 atcactgtat aaaaacttca gttaatactc atttgttttg tcgactcagt atataatgag
     2881 tgaccagtaa tcaggataat cacacttaca taacctgtaa aaaagaacaa gaagaataag
     2941 ccactaacat aatgcaaaat ggcatctgaa tcgatcgcag tttggcattc gaatcgatca
     3001 tgatttggcc caatccgtga tcgaatagag cggaatacgc aacataattc accagacaag
     3061 agcttacacc gaattacaga ttatgtatta atgtcaaaac tggcatattt tacagataca
     3121 atgaacatgc aacccatcaa tctaataggg gcggtatgta catctgccca tgaatgggat
     3181 ggcgctaaga atacaaaaca attagacata gatcacaaaa acacaccatt ctaattgaaa
     3241 aacaaaacaa acaaataaat catactatta tagaattttc tcttgccaga catagataat
     3301 acccctgcta attttgctaa tggttagcaa aattagcagg gtatataaag attattgacc
     3361 caaaacacac tcagatgaat tcacgacagc tcaggagggt tattcctgat gtcatcttca
     3421 caaataatgg ttaacgcatc atatatcatt ctgcgtctgt tcctgttttc ggccttatcg
     3481 ccatacaaca tcatcacatt tttaatttca tcgaccggtg atttcacata gtgaactaat
     3541 acaccaagct tctcaacaca ttgcagtaaa gtatatttct tactgcctgt gaaaagccct
     3601 atcatagttt ctttacgttc gcgactggat aatactttgt tttccataat gacttctgcc
     3661 agcgtcttac catcataaac atactcttta tctatcagaa tatttatctt atccagcacc
     3721 ttctctacgt catcaagcaa acctgtaata tatgggggcg caatatatgg tttcgtactg
     3781 atcttttcaa gcatacaatt atacatctta ataacacgca tcagggtaaa attgttcgca
     3841 tcatactgta tttcaccctc cttatataca ggatcaataa gtggtatttt ttcaataaat
     3901 gcatcctgat aggcttttac atggtcaagc caataaaaca acgagccata ctttatattt
     3961 tttctattta ctttaacctc cagtgctgtc cgaagcaacg ataatgcccc cctgacatat
     4021 cctgaaggta attcaccaaa caatcctttt ctttccatgt catcataatg ctgcagcaca
     4081 tcattaattt tatttttcct tatcagacaa atgtacaccg caagtgtttc gtagcagctg
     4141 gtcacatgaa cagcctcatt ctgcaactgc ccaaaatagc ttataatctt tttcagatat
     4201 gcgtcatcac aaccattctt atcattaata ttaaatacaa gatctttcag acgggaaata
     4261 tattcctgag atttatcatc aagtgaaaca ctataacgag acaatgttcc ttctgcactg
     4321 ttttgaaact tataaaacat ttccagtaat ttttttgcat aaggcgttgt atcaaagctg
     4381 gtaaagtcat aaagatattg cgcagcgtaa ccatacaaac catgaatcca gagtccttct
     4441 ctcaccaaca ctctgagata atatgcaagg ctttgaagta tcttcgtctt tcgttgtata
     4501 tcaagtaaga cacttaatga ataaaatgca ttatagaatt tatcaatatt cctgagcact
     4561 ttctccagtt catccttatc cagcttcttc tcccggtaat cctttttata ttcatttttg
     4621 atagccatga tatattgttg catcaggctc ttgcttttcc cgttatcctt ccgtatagtt
     4681 cctgtatatt tccccagagc atacagcttt ccattttcat gaaaatcttc cttgttgaat
     4741 cccagttcag ccagatattc ccgaagataa cgtttaacag cagcttcatc gctttctgta
     4801 tcagcgtgat caagacaagg acaatacgat ttctgtaaaa aattataaat atgataataa
     4861 aaagcagagt cattctctgg tctgtatgta ttatacatat ctacaacaag aaaaataacc
     4921 ccaaaagtgc gcagacaata gctgcgaagg aattcaggtt ttagtgtgga atgcactgga
     4981 taagaattca tcatgtaata aaaatgttca tatcgggcat atatgatccg ccgttgctcc
     5041 tgactgaaca ccccttcagc atcaataata cgactgatct cattataacg ttgatcccag
     5101 aatttttgat gcatttcaaa attggtactc ttccgcttgt gtgccggatt ctcgccttcc
     5161 ttaaacaacc ccaaaacagt acaaatatca tacaacatat ttgtaaatgt gcgcatatgc
     5221 tgtgctctcc ggttggtttg cattttatcg cagaggacat tctgagctta acgtatcaaa
     5281 ctgacacaac gacatgcaat gcatatggag aacaatatgt caaatcaaac cggacagctt
     5341 tacacgcctt cacgcatcct gcgtatggct gaacttaccc gactgctggg tatatcaaga
     5401 tctactattt acgtgaaaat aaatccggcc tcgaaatacc acgaccctac ctttcccaaa
     5461 ccaataaggc ttggtagtgc atccgtaggc tggcgggcat ctgccatcga tgagtggctg
     5521 atgttacata cggcaccagc ctggtctgaa cctgaaaaca aactaaagga acttaatgat
     5581 gacgacaaaa ctatcactcc ctgaacttga cacaactaaa ctgcctgaca ggtgccaggc
     5641 attacttgat gaaatgcatg aagaaacagg aatacgccgt gaaatacttc tgtctgttat
     5701 gctgacggtc atggctgcct cggtccagga tacgcatgaa gttgaacttt ccggagggca
     5761 acgtaccagc cttcagatat ttatgtgtct ttcatcagca tcaggcagtg gaaaaacctc
     5821 tgcctgcgca aaattaatcg ctcctgttca tgaaacagaa gaagaactac atcaggcata
     5881 tatcgacgat aaaaaaaact atgaccgaat gatggaaatg tggacaacag ataaaaaaat
     5941 cctggagcgg agatataaaa aggaaattga aagatccccg gaaaatgctg ctgctgcacg
     6001 agcagcactt gaaaagtgta tagcaaataa acccgaacct cccgtacaac aggttctcat
     6061 cgtgaatgac gcgacaccag aagggatagc tttgaaactc agccagtcac cctctcttct
     6121 gttgctgtct gatgaaggag gtactattct ggataagcgt ttcgaacgca aatcggcact
     6181 gtacaacaca ttatggagtg ggcaaccagt caacgttgaa cgggcatcca gacccgggtt
     6241 ccggctgaat gatgtccgtc tcactatact tatactgact caaccggtaa tatttaataa
     6301 gttttttact ctcactggcg atcagatccg gggcaatggc tttctggccc ggttgctatt
     6361 ttgcgaaccc ggagcaatta aaataatgac gacagaaccg catactgcca ctcctgttgt
     6421 gacgcaacaa tgtgcaagct gttttcgtaa gaaaagtttt ggcagtcaga tcagagactc
     6481 tctgagagca tccagagaaa gacgtgcaaa aagtaaagag cgcatctgca tgacattatc
     6541 aaatgcagca tcccgcgcac tggagcgctt tcacaaagaa aatatggata ctgtcagaca
     6601 gaatcctcat atgggtacat ttgaagacat tattgtcaga aaaagagagc aggttgtcag
     6661 gatagcagca cttctggaac tggagaaaga tccagactcg acagtaataa cgctggaaag
     6721 tactaatagc gccatttatc tcattgaatt ttatttcaag catcttatat ataaacttga
     6781 atcccttcgg gaaatatccc cggctgagaa acttgataaa tggcttcaga aaagaattat
     6841 cacaacagca ggttacatat tccagaaaag ttatatttta caatatgccc catatgctct
     6901 caggaaaaaa tgtgttctgg atgaagcact tgacatactg gcggaacaaa ggaaaataag
     6961 gattgatgat aacctggttg tttatatagg aaacacgatt acaccatccg aactggcaaa
     7021 aaaactaaat attcctgcat tcgatgccgg agttttcatt tgtgatcatc aaaacatact
     7081 gaagtatcac cgaaacagac tatagatact atcggacaaa aaataaatta tcacaagtgt
     7141 attattcgtt ataataatga ataatcgtta tctgagtgca atgccaggtt ttcattacga
     7201 actggcattg catttatttt tcaaaaaaga aagcagtaac catttggcta ctctgagtat
     7261 gccagtccat gaaactaagc gaattcctta gtgtttttag caagttgaga catcacataa
     7321 tccgcccatg cctgcatcat cggtacacgc tgctccagta gatcagtacg atgatatgcc
     7381 gcctcaacct tatttttcag cgtatgagcg agcgcccttt ccgccagatc ccgcgaatac
     7441 ccctgttcac tacaccagtc cctgaatgtt gagcgaaaac catgtgccgt ggcaactcgc
     7501 cccggaatgt cactgacggc tttcttttta cgcagaaaac ttgtcaacac catatcggaa
     7561 aggatctgct gctttctggg tgaagggaac accagttcat catgcaggcc acgtatattt
     7621 tccagaatgt aaatagcctg ccgggataaa ggaacacgat gctgtagcct agctttcatt
     7681 ctttctgcag gtatagtcca tacccgctta tgaaaatcaa tttcagccca gcgcattccc
     7741 ctggcttcgc ccgagcgagt tgctgtaagt atcaccatta ataacagtgc gcgggtaaca
     7801 ttataaggtt catcactata cacactggtc gccacaaaaa gcggtaactg cctccagggc
     7861 attgcgggtt ggtgttcatc acgtcctctt gtctgctgag gaagcaaatg gtcaaccaca
     7921 tcaacaggat ttgccacaca aaaaccgtgc gcccatcccc actgcataac aacatgaatg
     7981 cgctgtttaa cccggcttgc cgtttctgac aaggttaacc agactggacg cagtgtttct
     8041 gccacgtccg cagccgtaat cgaatccagc gtttttgctc ccagttgagg aaacgcgtaa
     8101 ttctcaagcg tcgataacca ctgccttaca tgctttggat tctcccatcc aggagacagt
     8161 tctgcatgta cacgcctggc tgcatcggca aatgttggga taacgacttt ctcagattca
     8221 gcctttttaa tctccagagg atcatcacct gcagcaagtt gctctcgcat tatccgggca
     8281 gtacgtgcag cttcagcaat actgacctct gggtaagttc ccaatccagc attacgtctt
     8341 ttttgtgtca ccggacttac ataacgaaaa acccatttcc cccgcccctt tactgaagaa
     8401 ggatgaaggg tcagtccggt aattccccca tggggcaatg gtttgtcatc aggtttgata
     8461 tgtcttgctt tcgtatccgt caatactgcc atacgctaat tcccgtcact ctggtatgcc
     8521 atccagtatg ccattagtcc ctcgcttcac taagaataca ttagataaca tcggacaaca
     8581 aaaagtgtaa tacattgttt ataaagaaat tatataacac ccctggatta agtcagattt
     8641 atttcaggcg gtccccctca ccgccatatt taaagaagag cgcgtacgaa agtacgggct
     8701 tttttttcgt atattgtaca caccgggtgc ccccgcccac gtctgttagg gcgaggggaa
     8761 attgtgcaca tcaactgtct ggttactcag tcaacaatca aaatgcgtaa gatagtacag
     8821 aacatatatg cgttatctgg tcacttcctg taacttcctc acttattaaa atgcccccaa
     8881 cggaaataca tgtcacatgc catcataaaa tcgttatgcc agaggaatcg acaatggaaa
     8941 aaatagtaaa ttaagcacgg tacatagcag agtaaaaaat aggcttcaaa ccaaatccgc
     9001 cattccacaa gcgttgccaa taccgacacc agcgtaatcc acatcatttc aataacccgc
     9061 aatatgaata accgaagcca aagcggcttg gcaaaaaagg tcgacaacgt attttcactg
     9121 gagaaaaaca gatcgatttc ctgcaaaaaa aacctgccca gaaaagcggg ccgcttggag
     9181 aagcgcagaa aaaacagatt aatcgccaac actacgccac aaagaatgag caatgcaatt
     9241 atcgatttaa acgttaacca ctgcccctct gtcacaaaag aatcactgtt acgcagcggg
     9301 ctgatagtaa gcaggttaac aaccgttgag atgcacaccg aaagcagcac atagcgagtc
     9361 agacttttat attttacttt tttcgctact gaaatagcga cctgcttgac ataactggtg
     9421 cggatcatcc gaaacagcag aaagatgacc aacggcccaa gtaacgaaat caggaacagc
     9481 gcatataacg agggtagaag ctggtatagc ccccaactca ttgcactcaa catgattaac
     9541 gacagaaatc ctgccaccag aggttgtgta agaaataatt ctattttgtg gttctgcttt
     9601 ttcttaaacc acgaaaaatc gatattcttc gtgacgcatt tataagccca gtacgccttg
     9661 agatcaaaaa taaaattata cagcaccagg gccagaatcc cactaactac accaatatcc
     9721 tctgatggaa aagtaaaccc tgcatttttc caataaaagc ctatcagaat aagtccaata
     9781 gcataaatgt atatcagatt tcgtttatcc tgaaagcgaa taaaagttaa ataatcagga
     9841 atcatttgaa tacctttgca tcactaaatg ctttacgaca atgcataact cgttgctgat
     9901 ccacgccagc aaaatcattt aacatattcc ccaaataggg cttattactt atttcaagga
     9961 aagctaccgg ggttggcata ttcagaatat gtatcgagag attcagtcca tttcaccgta
    10021 tgagttaagt gtaatgagag attttctttg ataattgacg gagcgacctc gctttttgct
    10081 gttacgttca tcagaacctg atgctctggc gaagcgatat ccagtccggc aagataatcg
    10141 cgcattgcct gaacgccgtc ttccatcaaa cgcgtgtgcc aggcaccgct cacaccgagt
    10201 ttaaccggtt cgtatccagc ggccatcagc agcgtggtaa attcattcaa cgcagcctgc
    10261 gttcccccaa taacctgctg gcgcggcgtg ttatcacagc taatatccag cgcaatgcct
    10321 gattccgtaa tcatcgtctg cagttgttcg cggttgatgc ctttaaccgc
//
LOCUS       DAJTSF010000124        10105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000124 DAJTSF010000000
VERSION     DAJTSF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.124"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            123..254
                     /locus_tag="OO335_003472"
     CDS             123..254
                     /locus_tag="OO335_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR6003715.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            514..1509
                     /gene="pgl"
                     /locus_tag="OO335_003473"
     CDS             514..1509
                     /gene="pgl"
                     /locus_tag="OO335_003473"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6003716.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1550..2503)
                     /locus_tag="OO335_003474"
                     /pseudo
     CDS             complement(1550..2503)
                     /locus_tag="OO335_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2687..3739
                     /locus_tag="OO335_003475"
     CDS             2687..3739
                     /locus_tag="OO335_003475"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR6003717.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3815..5248
                     /locus_tag="OO335_003476"
     CDS             3815..5248
                     /locus_tag="OO335_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR6003718.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5431..7611
                     /locus_tag="OO335_003477"
     CDS             5431..7611
                     /locus_tag="OO335_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6003719.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7752..9035)
                     /locus_tag="OO335_003478"
     CDS             complement(7752..9035)
                     /locus_tag="OO335_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6003720.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9170..10051)
                     /locus_tag="OO335_003479"
     CDS             complement(9170..10051)
                     /locus_tag="OO335_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6003721.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2452 a         2359 c         2552 g         2742 t
ORIGIN      
        1 tttttttctt taaaaaaaca gtacattcta ttttataatg actgtgtatt cccatggaag
       61 gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca gggggattga
      121 ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat ctacaacgta
      181 atgcgtttat ttgtgccgga agtgagttga ttgttgcgcg aatgggaagt tggccagaag
      241 ttgttatggc atagtgttta cggtcgttga tacaggtaaa caaaagcggg gagagtcata
      301 ctccctgaat agtcgcgaaa atatctgtgg cattgtcaaa ttcgtgtcgg tgactaatat
      361 tttagtaata tgccattcga aatggagtgc taacttattg atttaattga tgtgcgatat
      421 tgtattttat cgtgttttgt tgtctgtttt ttatccagta agtgttgtta tatgtatgaa
      481 agataaggga tttatcattt ttgaggtgtg ttcatgaagc aaacagttta tatcgccagc
      541 cctgagagcc agcaaattca cgtctggaac ctgaatcatg aaggcgcact gacgctgaca
      601 caggttgtcg atgtgccggg gcaggtgcag ccgatggtgg tcagcccgga caaacgttat
      661 ctctatgttg gtgttcgccc tgagtttcgc gtcctggcgt atcgtattgc cccggacgat
      721 ggcgcactga cctttgccgc agagtctgtg ctgccgggta gtccgacgca tatttccacc
      781 gatcaccagg ggcagtttgt ctttgtaggt tcttacaatg cgggtaacgt gagcgtaacg
      841 cgtctggaag atggcctgcc agtgggcgtc gtcgatgtag tcgaggggct ggacggttgc
      901 cattccgcca atatctcacc ggacaaccgt acgctgtggg ttccggcatt aaagcaggat
      961 cgcatttgcc tgtttacggt cagcgatgat ggtcatctgg tggcgcagga ccctgcggaa
     1021 gtgactaccg ttgaaggggc cggcccgcgt catatggtat tccatccaaa cgaacaatat
     1081 gcgtattgcg tcaatgagtt aaacagctca gtggatgtct gggaactgaa agatccgcac
     1141 ggcaatatcg aatgtgtcca gacgctggat atgatgccgg aaaacttctc cgacacccgt
     1201 tgggcggctg atattcatat caccccggat ggtcgccatt tatacgcctg cgaccgtacc
     1261 gccagtctga ttaccgtttt cagcgtttcg gaagatggca gcgtgttgag taaagaaggc
     1321 ttccagccaa cggaaaccca gccgcgcggc ttcaatgttg attacagcgg caagtatctg
     1381 attgccgctg ggcaaaaatc tcaccacatc tcggtatacg aaattgttgg cgagcagggg
     1441 ctactgcatg aaaaaggccg ctatgcggtc gggcagggac caatgtgggt ggtggttaac
     1501 gcacactaac cgctgattta accggcgcag tctctcctgc gccggtgtat taacttatct
     1561 cctgtaacgc atgtctctgg cgttcgacga tattggtcca caaattgtct ttatcgtcag
     1621 tccacaaatt aatcagcaag gaaaaaaagc gttcagctgc cggagaaagc acggcatctt
     1681 tgcggcgaat aatccccaat gtccgacgta taacgggttc caccagtggg ataccaataa
     1741 tcgaagaata gggcgcatgg ggcattgcca gaccaggaag cgccgaaatc cccagtcctg
     1801 cctccactag tcctaatgac gtcgaaagat gacgcacttc gtaaaaccag tccagcttcc
     1861 agggcttgtc ggctagctgt tgttctatca acaatcggtt gccgctggag gaacgtacgc
     1921 caatcatttt gtaacccact aactcctgcc attctacaag ctactttttg gccaatggat
     1981 ggtcacgccg acaggccagc acgaatggct cgttgaccag tggggtaaaa tcaatggatg
     2041 aatttgtgac gttgttcatg tttatgccaa agtctgattc gttacacagc accgattcca
     2101 tgcaattatt cgtgccttgt tccagaatcc gcactttaat attgggatac agctcattaa
     2161 atttaccgat tgccagcggt aaaaaataaa atactgcggt cggaatacac gctaatgtca
     2221 ccataccacg atgataagca ttcatatcac gaatattaaa aagcgtttca tcgaatttct
     2281 ttattaattc ccgcgcctcc gggagcaacc tttttcctgc ttttgttaac gtaaccttgc
     2341 gagttgtacg ctcaaatagt tgaatatgta aatcttcctc catctttttt attcttcgcg
     2401 ttaatgcagg ttgcgtaata ttgagtaatt tagcggcatt gttaaatgaa ctggactctg
     2461 ccagtatgac aaatgccttc atacttgata attcatgctt cattattact ccggaaaatg
     2521 gaagcgacga ttttgggggg ctggtcgtta aaaattttaa ctgcatttag ccaacttaaa
     2581 ttaatgaaaa aatgttatta atcgttgagc taaagtcatt agagatgctt tgtccttaat
     2641 gtaaccatat cgcaataagt tatgttttta aattgagggc attattatga aaaaaatacc
     2701 ctgcgtgatg atgcgaggtg gaacctcgag gggcgcgttc ctgttagcgg aacatttacc
     2761 cgaagatcaa acgcagcgcg ataaaatatt gatggcaatt atgggttccg gtaacgatct
     2821 ggaaattgac ggtattggcg gcggtaatcc actgaccagt aaagtcgcca ttattagccg
     2881 ttccagcgat ccgcgtgctg atgtcgatta tctgtttgct caagttatcg tccatgagca
     2941 acgtgtcgat accacgccta actgcggcaa tatgctgtct ggcgtagggg cattcgccat
     3001 tgaaaatggt ttgattgcag cgacttcgcc agttacccgt gtacgtatcc gcaacgtcaa
     3061 tacgggtacg ttcatcgaag ctgatgtgca aacgccaaat ggtgttgtcg agtacgaggg
     3121 tagcgccaga attgacggcg taccgggtac tgccgcaccg gttgcgctca ctttcctgaa
     3181 tgccgctgga acgaaaactg gaaaagtttt cccgactgat aatcagattg attattttga
     3241 cgatgtcccg gtgacctgta tcgatatggc gatgccagtc gtcattattc cggctgaata
     3301 tctgggaaaa acaggttatg aattaccggc ggaactggat gccgacaaag cattattagc
     3361 ccgcattgaa tctatccggc tacaagcggg taaagcaatg ggcttaggtg atgtcagtaa
     3421 tatggttatc cctaaacctg tgcttatttc tccagcgcag aaaggcgggg caattaatgt
     3481 gcgttatttt atgccgcatt cttgccatcg cgcgctggcg ataaccggtg ctattgctat
     3541 ttccagtagt tgtgcattgg aaggcaccgt cacccgacaa atcgtccctt ctgtaggata
     3601 tggcaatatc aatattgaac accccagtgg tgcgctcgac gttcatttaa gtaatgaagg
     3661 tcaggatgcc acgacgttac gcgcatctgt tattcggaca accagaaaaa tattttccgg
     3721 tgaagtttat cttccctgaa aaaattcgtt gtcaggataa ggacaatcaa taaaggactt
     3781 ctgtatgagt catacagaaa gaacaggatt ttaaatgaat aagaaatcgt tatggaagct
     3841 aattctgata ttagcgatcc catgtattat tggttttatg ccagctccgg caggattaag
     3901 cgaactggcg tgggtgcttt ttggtattta cctggcggcc attgtgggtc tggttatcaa
     3961 gcctttcccg gaacctgtcg tactgttaat tgccgttgct gcctcaatgg tggtggtcgg
     4021 taacttatcc gacggtgcgt ttaagaccac cgccgtatta agcggttact cttcaggtac
     4081 tacctggctg gtgttctcgg cgtttacctt aagcgccgca tttgtgacca ccggtttagg
     4141 taaacttatt gcctatctgc tgattggtaa aatcggtaac accatgctgg gtctgggtta
     4201 cgttacggta ttcctcgatc tggtactggc tccggcaaca ccgtctaaca ccgcgcgtgc
     4261 gggcggcatt gtgttaccga tcatcaacag cgtggcggtg gctttggggt ccgaaccgga
     4321 aaaaagtccg cgtcgtgtcg gacattacct gatgatgtcc atttacatgg tcaccaaaac
     4381 caccagctat atgttcttta ccgcaatggc ggggaacatt ctggcgctga aaatgatcaa
     4441 cgacattctg cacctgcaaa ttagctgggg tggatgggcg ctggccgccg gattgccggg
     4501 catcattatg ctgctggtca ccccgctggt aatttacacc atgtatccac cagaaattaa
     4561 aaaggtggat aacaaaacca tcgctaaagc gggccttgcc gaactgggac cgatgaaaat
     4621 ccgcgaaaaa atgctgctcg gtgtctttgt gctggcgcta ctgggctgga ttttcagtaa
     4681 gtctctgggg gttgatgaat ccactgtggc aatcgttgtt atggcgacca tgctgctgct
     4741 gggtatcgtt acctgggaag acgtggttaa aaataaaggc ggctggaata ccttaatctg
     4801 gtacggcggt attatcggct taagctcctt attatcgaaa gttaaatttt tcgaatggtt
     4861 agctgaagtc tttaaaaata acctggcatt tgatggtcac ggtaacgttg ctttcttcgt
     4921 tattattttc ctcagcatca tcgtgcgtta tttcttcgct tccggtagtg cctatatcgt
     4981 tgctatgtta cctgtatttg ccatgctggc gaacgtctcc ggcgcaccgt taatgttaac
     5041 cgcgctggca ctgttgttct ccaactccta tggcggcatg gttactcact atggcggcgc
     5101 ggcaggtccg gtcatctttg gcgtgggtta caacgatatt aaatcctggt ggttggtcgg
     5161 tgcggtactg acgatattaa ccttcttggt gcatatcacc ctcggcgtgt ggtggtggaa
     5221 tatgctgatc ggctggaaca tgctgtaaat atactcgtca tacttcaagt tgcatgtgct
     5281 gcgtctgcgt tcgctcaccc cagtcactta cttatgtaag ctcctgggga ttcactcact
     5341 tgtcgccttc ctgcaactcg aattatttag agtatatcca tttattatct ttctgcgcac
     5401 ttcacggtgc gcagatatct ggagcatttg atgatcaagt tatctgaaaa aggcgtgttt
     5461 ctcgccagta ataacgaaat aattgccgaa gaacatttca ccggcgaaat taaaaaagaa
     5521 gaagcaaaaa aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat
     5581 atggataaac ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt
     5641 attgtacaga ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc
     5701 aactgccata actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt
     5761 ggtttatcgg cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc
     5821 catcaatata tgcgtgagat gatggctggc gggggcaaaa tgatcctcgg gtcagacagc
     5881 cacacccgtt acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta
     5941 aaacagctgc ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg
     6001 accggaaaac ctgcgccgta tgtggggcca caggatgtgg cgctggctat cattggcgcg
     6061 gtgttcaaaa acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc
     6121 gcgctctcta ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta
     6181 agctccgtct ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggt
     6241 caggattact gccagcttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt
     6301 gatttaagcg ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa
     6361 atcgacacac tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa
     6421 cgcgtggcgc acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg
     6481 aaagtgcagc aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg
     6541 gcggcgaatg cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcggtttac
     6601 ccgtcatcac agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt
     6661 ggcgcaggcg caatcatcag aaccgcattc tgtggcccat gctttggcgc gggcgatacg
     6721 ccaatcaaca acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc
     6781 tctaagccag ccaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct
     6841 gcgactgcgg caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac
     6901 gtgccggagt acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg
     6961 aaaggggcaa ctcagcaacc gctgatttac ggaccgaaca ttaaagactg gccggaattg
     7021 ggtgcgctga ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtcacc
     7081 accaccgacg aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt
     7141 ctggcgggga atgtcagcga gctgacagag gtgtttgcgc gcattaagca gattgctggt
     7201 caggagcata ttgatccgct gcaaactgaa attggcagca tggtctatgc ggtgaaacca
     7261 ggcgatggtt ctgcgcgtga acaggcggcg agctgccagc gtgtgattgg cggtctggcg
     7321 aatattgccg aggagtacgc gactaaacgc taccgttcta acgtcatcaa ctgggggatg
     7381 ttaccgctgc agatggcgga agtgccaacc tttgaagtgg gggattacat ttacatccct
     7441 ggcattaaag cggcgctgga taatccgggt acgacgttta aaggttatgt gatccatgaa
     7501 gatgcgccgg taacggaaat tacgctttac atggaaagcc tgacggctga agagcgcgag
     7561 attatcaagg cgggtagttt gattaacttc aataaaaacc gtcagatgta aaaagcgcca
     7621 tgtgaatgta ggtcgcattc ggcacttatt gtcggatgcg atgctggcgc atcttatccg
     7681 acctacaaat cgcatcgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcaaata
     7741 gttaatagct cttacttctt ctcctctgca accactttgc tgcccacgcc gcggttattg
     7801 tattcccaca tgcggttgta gttagtgtca ttcagattgc gctgtatttc gtcgttatca
     7861 tctacgctgc cggtattacc cgcaaacgga cgattagaga tcaccgcatc ggcccacggt
     7921 ttagccgtgt taaaaccttc gttgatggcg ctatcacgga tcaccacctg accgttggta
     7981 ttagcatcaa catccagcga gcggcccagt tgcgccacgc catcaccgga agcattgaaa
     8041 cggctgttta cggcgaggaa accgtaatag atgttagaca gcgtagccgg tgcaaacaca
     8101 tatgcttctt gctgggtacg tgagttcacc acgcggaatt cggtgttatc gaacaccact
     8161 gcgccgcgac cagaaacgat atccacatcc ccttcaatat agctgttggt caccagcgta
     8221 cgcggctgac ggttggtttc cagacggttc tgcacaccgc tgttggtgac aaagaaggtg
     8281 ttctgacgac cgagaatgtt aacgttgtta atctgtactt ggtcaccgtc agtacgcagt
     8341 gctaccgccg gatggttacc tgcatctacg ctatcgccca gcgtgttttc gatggtcaga
     8401 ttttgcagtt gcagtccatt gttttgtgac cagaagaccg cagaacacag aacaccgata
     8461 ctgtcgctgc gtttgctctg gcagctatcg tacatatacc acgctggttt acctggcata
     8521 tatttgccgc gcgggttgac gtcgtgacgc cagtcggcag ggctcatgcc cccatcaagg
     8581 gaaagcccaa tcttcacatc aatcggtttt tcacctgtac cgtacagagt gattccacct
     8641 ggagcagcag ggacatacac cgttccctga tactcaccag gcatcacggc aatatactgg
     8701 cgcttgttgg tgcgcttgat aattgccgca tctaccgccg cctgaatcgt ggtatgcgtt
     8761 acaccttgag tacccgccgg gccgacaaca aagtcaggtt gcgcaggcag ggttatcggg
     8821 gacggattcc acgctgcagc acctggtgtc agggatgcaa aatagtgttg cgcatcgaaa
     8881 ttcagcgctt cttttgccga cagaatcggg cgagaagagg taccaggcgc agtttgatca
     8941 gaaggacgtt gatcgggtgg tgttgagcta caggcggtca gcgtcacgcc aaaagccaat
     9001 gccagcgcca gacgggaaac tgaaaatgtg ttcacaggtt gctccgggct atgaaataga
     9061 aaaatgaatc cgttgaagcc tgctttttta tactaagttg gcattataaa aaagcattgc
     9121 ttatcaattt gttgcaacga acaggtcatt atcagtcaaa ataaaatcat tatttgattt
     9181 caattttgtc ccactccctg cctctgtcat cacgatactg tgatgccatg gtgtccgact
     9241 tgtgcccgag aagatgttga gcaaacttat cgcttatctg cttctcatag agtcttgcag
     9301 acaaactgcg caactcgtga aaggtaggtg gatccccttc gaaggaaaga cctgatgctt
     9361 ttcgtgcgcg cataaaatac cttgatactg tgccggatga aagcggttca cgacgagtag
     9421 atgcaattat ggtttctccg ccaagaatct ctttgcattt atcaagtgtt tccttcattg
     9481 atatcccgag agcatcaaca tgcaatgttg tagggatggc aatttttacg cctgttttgc
     9541 tttgctcgac ataaagatat ccatctacga tatcagacca cttcatttcg cataaatcac
     9601 caactcgttg cccggtaaca acagccagtt ccattgcaag tctgagccaa catggtgatg
     9661 attctgctgc ttgataaatt ttcaggtatt cgtcagccgt aagtcttgat ctccttacct
     9721 ctgattttgc tgcgcgagtg gcagcgaccg ggtttgttgt tatatggcct tcagctattg
     9781 cctctcggaa tgcatcgctc agtgttgatc tgattaactt ggctgatgcc gccttgccct
     9841 cgtctatgta tccattgagc attgccgcaa tttcttttgt ggtgatgtct tcaagtggag
     9901 catcaggcag ccccctcctt attgctttaa ttttgctcat gtaatttatg agtgtcttct
     9961 gcttgattcc tctgctggcg aggatttttt cgtagcgatc aagccatgaa tgtaacgtaa
    10021 cagaattatc actgttgatt ctcgctgtca gaggcttgtg tttgtgtcct gaaaataact
    10081 caatgttggc ctgtatagct tcagt
//
LOCUS       DAJTSF010000125         9935 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000125 DAJTSF010000000
VERSION     DAJTSF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.125"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="OO335_003480"
     CDS             complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="OO335_003480"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR6003722.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="OO335_003481"
     CDS             complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="OO335_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR6003723.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="OO335_003482"
     CDS             complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="OO335_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR6003724.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="OO335_003483"
     CDS             complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="OO335_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6003725.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="OO335_003484"
     CDS             complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="OO335_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6003726.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="OO335_003485"
     CDS             complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="OO335_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6003727.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="OO335_003486"
     CDS             complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="OO335_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6003728.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="OO335_003487"
     CDS             complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="OO335_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6003729.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8990..>9935)
                     /locus_tag="OO335_003488"
     CDS             complement(8990..>9935)
                     /locus_tag="OO335_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6003730.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2562 a         2586 c         2294 g         2493 t
ORIGIN      
        1 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat
       61 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt
      121 catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc
      181 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg
      241 gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
      301 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat
      361 tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg
      421 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt
      481 gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg
      541 ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
      601 agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac
      661 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
      721 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
      781 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata
      841 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
      901 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc
      961 accttccacc tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt
     1021 cataattaac tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat
     1081 ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg
     1141 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     1201 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     1261 tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     1321 ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc
     1381 aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc
     1441 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     1501 tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact
     1561 ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc
     1621 aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt
     1681 gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc
     1741 ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc
     1801 atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac
     1861 ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat
     1921 aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat
     1981 acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc
     2041 cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat
     2101 catttcgctt atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac
     2161 catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag
     2221 tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga
     2281 gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg
     2341 cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc
     2401 agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa
     2461 cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt
     2521 ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag
     2581 accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc
     2641 tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac
     2701 cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc
     2761 caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc
     2821 ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc
     2881 cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg
     2941 cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt
     3001 tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt
     3061 ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac
     3121 gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt
     3181 aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa
     3241 gaatacgcca ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttctt
     3301 cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg
     3361 tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa
     3421 taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg
     3481 cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt
     3541 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
     3601 cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca
     3661 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
     3721 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
     3781 caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
     3841 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
     3901 aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac
     3961 aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat
     4021 tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct cgcagttatt
     4081 aactttgtgt aattttagga atttataaag ttatatataa caaatcccaa taattaagtt
     4141 attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag tttatccagc
     4201 tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat aaactcacaa
     4261 atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag cgcggtaagt
     4321 acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg aacaatacct
     4381 tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc gtcaatgtcc
     4441 atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg gcccagcgac
     4501 tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc agtacggcag
     4561 attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact gatcaccata
     4621 accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac gcaggcttca
     4681 tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg cttcgccaga
     4741 tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc caccatgccg
     4801 cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg gccggaaata
     4861 tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa cgaccggtga
     4921 tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg ttgctgaact
     4981 caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca gcctgctgca
     5041 tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc agctcttcaa
     5101 tacgcccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc agcacttccg
     5161 ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca gagagagagt
     5221 aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg gtcaatgccc
     5281 actgcacgcg ctcgtccatg tcggcacggg agagcttctc aaacagacga acgccaaagg
     5341 cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc atgcccactt
     5401 tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca tcaagcagaa
     5461 tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag gtacgcagca
     5521 gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt ttggcgatat
     5581 ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa ttacgaacct
     5641 gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca tcgtacctga
     5701 tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc gcatccggaa
     5761 aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt cagcagcagg
     5821 acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc cgcaaacggg
     5881 ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat catgtccgtg
     5941 ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc ggcaatacgg
     6001 gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag cgtaatcgcg
     6061 gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag gctgtccggc
     6121 accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa cagcgccagc
     6181 gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat ggtgtagaca
     6241 aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa gcgaatcact
     6301 tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat aatgcccagc
     6361 ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag ggcgttcgcc
     6421 agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc cagcgacata
     6481 ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa caggccgaag
     6541 gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg acgcgcctgc
     6601 attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat agccattagc
     6661 gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg aggacgatga
     6721 aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc agaccggatt
     6781 ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc atgtacagcg
     6841 aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg gtttcaccga
     6901 gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg aacggaagaa
     6961 cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac tctttcatca
     7021 tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga ataatcatga
     7081 tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca gagaacagcg
     7141 cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag taaacggcga
     7201 acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga atggctgcca
     7261 gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc tcagtcagga
     7321 acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac gaagtcacca
     7381 acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc cactctttgg
     7441 tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg atcagagaga
     7501 caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc agcacgctga
     7561 aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca gccataagtt
     7621 actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg cgtaaaacac
     7681 cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt taatattggt
     7741 cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt aatccaggtc
     7801 gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca cctctgtgcc
     7861 ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg taataggcca
     7921 tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc agtctgcacc
     7981 ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat cagcggagat
     8041 cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat aaccaattgc
     8101 acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc ccagaccgat
     8161 cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt tcactttcgc
     8221 caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta ctacagcaat
     8281 gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat catcccactt
     8341 cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca gttcgccaga
     8401 cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg ggaactggaa
     8461 cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac caaaatcaac
     8521 ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt agttaacttt
     8581 attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg cagggaaggt
     8641 tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca tcgataaggt
     8701 cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc ctgggaggat
     8761 tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt gacagttata
     8821 tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta ctgattttaa
     8881 atgtgaagat gaaaattcca catgccatct ttttgtcatg atgcatttaa aagtgataaa
     8941 gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat tagcgcacaa
     9001 tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga gctccagtaa
     9061 ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac actgttgatt
     9121 tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta tttttgtcgt
     9181 aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc gtattgggtt
     9241 cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg ttttcacact
     9301 caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca acagatgaaa
     9361 aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta tttatttcgc
     9421 aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc tgtgagtaat
     9481 ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt tcaggaataa
     9541 tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta gccccactgg
     9601 atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac gggttgttag
     9661 catgatattc aaaaggtgca ttcacatacc caaccccaag aatatctata gcaatcccaa
     9721 catcaaggtt cggattaaga tcataataat taatattaga gtgaacccca gcataagtta
     9781 atgcctggtt tatttttgac gtataataga tataggggaa ttctcctacg ttaccagtat
     9841 cacaatgaca gcgtccaaga tatgaggatc cattagttac agtggtgtta aatgtcgttc
     9901 caaccttatt ttcgttcgcg gtaataagat atccc
//
LOCUS       DAJTSF010000126         9773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000126 DAJTSF010000000
VERSION     DAJTSF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.126"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="OO335_003489"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="OO335_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6003731.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="OO335_003490"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="OO335_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6003732.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="OO335_003491"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="OO335_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6003733.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="OO335_003492"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="OO335_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6003734.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="OO335_003493"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="OO335_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6003735.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="OO335_003494"
     CDS             complement(6031..6360)
                     /locus_tag="OO335_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6003736.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="OO335_003495"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="OO335_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6003737.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="OO335_003496"
     CDS             7998..8171
                     /locus_tag="OO335_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6003738.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="OO335_003497"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="OO335_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6003739.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2286 c         2433 g         2491 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttgcaaaa accgtgccct taaatattgg atc
//
LOCUS       DAJTSF010000127         9761 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000127 DAJTSF010000000
VERSION     DAJTSF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.127"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            383..1501
                     /locus_tag="OO335_003498"
     CDS             383..1501
                     /locus_tag="OO335_003498"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6003740.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="OO335_003499"
     CDS             1567..1815
                     /locus_tag="OO335_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR6003741.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="OO335_003500"
     CDS             1880..2248
                     /locus_tag="OO335_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6003742.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="OO335_003501"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="OO335_003501"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6003743.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /gene="ybdG"
                     /locus_tag="OO335_003502"
     CDS             3103..4350
                     /gene="ybdG"
                     /locus_tag="OO335_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6003744.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="OO335_003503"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="OO335_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6003745.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="OO335_003504"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="OO335_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR6003746.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="OO335_003505"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="OO335_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2387 a         2705 c         2368 g         2301 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagctg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg c
//
LOCUS       DAJTSF010000128         9721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000128 DAJTSF010000000
VERSION     DAJTSF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.128"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(133..453)
                     /gene="psiF"
                     /locus_tag="OO335_003506"
     CDS             complement(133..453)
                     /gene="psiF"
                     /locus_tag="OO335_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR6003747.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(572..1987)
                     /gene="phoA"
                     /locus_tag="OO335_003507"
     CDS             complement(572..1987)
                     /gene="phoA"
                     /locus_tag="OO335_003507"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR6003748.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2088..2348)
                     /gene="iraP"
                     /locus_tag="OO335_003508"
     CDS             complement(2088..2348)
                     /gene="iraP"
                     /locus_tag="OO335_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6003749.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2530..2733)
                     /locus_tag="OO335_003509"
     CDS             complement(2530..2733)
                     /locus_tag="OO335_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003750.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2811..3905
                     /gene="ddlA"
                     /locus_tag="OO335_003510"
     CDS             2811..3905
                     /gene="ddlA"
                     /locus_tag="OO335_003510"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6003751.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3929..4141)
                     /locus_tag="OO335_003511"
     CDS             complement(3929..4141)
                     /locus_tag="OO335_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6003752.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4401..4709
                     /locus_tag="OO335_003512"
     CDS             4401..4709
                     /locus_tag="OO335_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6003753.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4768..5862)
                     /gene="yaiW"
                     /locus_tag="OO335_003513"
     CDS             complement(4768..5862)
                     /gene="yaiW"
                     /locus_tag="OO335_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6003754.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5875..7095)
                     /gene="sbmA"
                     /locus_tag="OO335_003514"
     CDS             complement(5875..7095)
                     /gene="sbmA"
                     /locus_tag="OO335_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6003755.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7447..8604
                     /gene="ampH"
                     /locus_tag="OO335_003515"
     CDS             7447..8604
                     /gene="ampH"
                     /locus_tag="OO335_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6003756.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8605..9108)
                     /locus_tag="OO335_003516"
     CDS             complement(8605..9108)
                     /locus_tag="OO335_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6003757.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9196..>9721)
                     /locus_tag="OO335_003517"
     CDS             complement(9196..>9721)
                     /locus_tag="OO335_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6003758.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2419 a         2481 c         2317 g         2504 t
ORIGIN      
        1 ggaaaggctg ggccacatcc ctccggcaga agcagaaaaa gcttattatg cttccatcgg
       61 aaacgatgat ctggcagcct gagttcacag ataaaacact ctccaggaaa cccggggcgg
      121 ttcaatcagg tatcaggcgg ctttcttgag gcaggcactc ataaacttat tacgatcatc
      181 acctttcaga gcttgttgcg ttgcttgatt attacattca cgcatctttt gctgctgtgg
      241 cgtcaaactt ttttcaccag gcgcagactt gctgttcttc aggcaatcac tcatgtaggt
      301 cttacgagca tcccctttca acgcctgcgc cgtcgcctgc tgattacagg aggtcatacg
      361 ctgttgttgt ggggttaagg ttctctcggc agcgccgacg gtggttaaaa aaaccagacc
      421 aaaaagcaag gtaaccagta atgttatttt catagcacca tccctcttca tgttttaacc
      481 atgagcgttt gcgcccgtga tctgccatta agtctggttg ctaacagcaa aaaaaccacc
      541 cggcagcgaa aattcactgc cgggcgcagt tttatttcag ccccagagcg gctttcatgg
      601 tgtagaagag atcggtctgg tcggtcagtc caacaacatt ggcggcatgc gggccatacg
      661 ccgcaatacg caactgactg ccggtatgtt cttgtgaatc ctcttcggaa ttcccgtaac
      721 tcatcaccat cactgcgcca tctttagtat ttagcgcctg agtgaggccc ggcgctttgg
      781 tatccggcgc aacaacctgg ctggcgtggg cgtgatcagc ggtgactatg accagcgtgt
      841 taccctcctt tttagcgaat tccagcgccc gttgtacggc ttcatcgaga tcgaccgtct
      901 cgccaatttg cccacaaggg ttcgcagcgt gatcctgttt atcgattgac gcaccttcaa
      961 cttgcaggaa aaatcctttc tcatttttac tcaacaattc aatggctttg tcggtcatct
     1021 gtgccagggt tggtacactg tcattacgtt gcggattagg cgtataggtg actgcgggct
     1081 tatcgatatt gccgtggtac gttgctttcg gccctagcca gcgcactggc atattgccgt
     1141 cagcaaacag gccaagcagg ggtttttgct gattcgcttc cgtcaccgaa ttcagtgagg
     1201 cagcatcgtt caccaactga taaccacgcg cctgtgcctg ttcacgcagc gtttttccct
     1261 gccattcacc agcggttgcc gtttcagcaa aggtttttgc gccgccgcca agcgtaacgt
     1321 cggcacgagc gttaagcagc tgttcggtaa tcgatccttt tccgcctttt tccagagcgt
     1381 tacccggaca tttttcactg gtcgcgctcg gaccgtagca tttgcgcgaa gtcacatgtg
     1441 ccaccagcgc agcgggcgtg gcatcctgca actctgcggt agaaacgtta ccggtcgcca
     1501 gacctgcggc ttttgccatt tccagaatcg ttgggtgatc tttttcgtga atatcgacgc
     1561 ccagcgcgcc gttataggtt ttgacatcgg ttgaccaggc ggttgctgat gcagccgagt
     1621 cggtgacgta gtccggtttg ccagtttttt tattcagcgc atagtgagtg tattgcccgg
     1681 taagcggtaa ggcatcgata cctttaaaaa agccgcccgc accttcggca taattacgtg
     1741 cggcagtaat ttccgaatcc cccatcccat cgccaatcag caaaataata ttttttgcag
     1801 gtttatcgct aagagaatca cgcagagcgg cggtctgatc acccgttaag cggcgagcac
     1861 cgccgggtgc agtaatatcg ccctgagcag cccggttttc cagaacaggc atttctggtg
     1921 tccgggcttt tgtcacaggg gtaaacagta acggtaagag tgccagtgca atagtgcttt
     1981 gtttcacttt attttctcca tgtacaaata cattaaaaaa taaaaacaaa gcgactataa
     2041 gtctcggccg tgacaacttt atgacagctg ttgaaaagat taacttttta ctgacaagga
     2101 tgcttcaata acttctttac gtaatcgcgc agcagctctg tatcgtcgtc aggaatgctg
     2161 gcatcgggct ttacctcgta cagcgccccc tctacctgat caatcaaccg ctgttggtca
     2221 ttttgcgcca tattgcgaag cattgcagtg acgataatct ccaaagcttc tacctgcgca
     2281 cacagttctt tcgactcttc ttctttttgg gcaagcttaa ataacaactc agcaatgaga
     2341 tttttcatgt ctgtatttcc ttatccaaag tatggagaag ttaacattat gtttcacaac
     2401 ttcatagtgt ctggaagtat tatttttatg acaaaggaaa tactttgcgc aaatattttt
     2461 tgtttgattg ccagcgaaac gtttctctta tttaaataat tacccacaca aaatataggc
     2521 ttttaattat caacctttat tataaataaa agctatgaaa tatattctct tcgttaactt
     2581 ataattaaga gaaaaaaact caccgcattg ccatttaagc caggaataaa ttagcttccc
     2641 gttcatcatt tttgtgatct tgcgcacatc tttgagataa cgcactttct tcaccagtac
     2701 gttgcacgct ttgtcagcag aagattgcaa caatctttca gaaggccgcg aatcccccgt
     2761 tttgtcgtgg taatgtatgc actttgtgtt agacgggata ggtttttaag atggaaaaac
     2821 tgcgggtagg aatcgttttt ggtggtaaat cagcggaaca tgaagtgtct ctgcaatcgg
     2881 caaaaaacat tgtcgatgcc attgataaaa gtcgcttcga cgttgtgctg ctgggcattg
     2941 ataaacaagg gcaatggcac gtcagcgatg ccagcaatta tctgctaaat gcagacgatc
     3001 ctgcccatat tgcgttgcgc ccttcggcga ccagccttgc gcaggtgcca ggtaaacatg
     3061 agcatcagct tatcgacgcg caaaacggtc agccgttgcc gacggtggat gtcattttcc
     3121 cgattgtcca cggtacgctg ggcgaagatg gttccttgca gggaatgctg cgggtcgcca
     3181 atttaccgtt tgtaggttct gatgttctgg cttcagcagc ctgtatggat aaagatgtca
     3241 ccaaacgtct gctacgcgat gccgggctga acattgcgcc atttattacc ctgacgcgcg
     3301 ctaatcgtca caacatcagt tttgccgaag tggagtctaa actggggtta ccactgtttg
     3361 taaaaccggc taatcagggc tcttctgttg gtgtcagcaa agtaaccagt gaagaacagt
     3421 acgcaattgc cgtcgatctg gcgttcgagt tcgatcataa agtgatcgtt gagcaaggga
     3481 tcaaaggtcg tgagatcgaa tgcgcagttc tgggcaacga caatccgcaa gccagcacct
     3541 gtggcgagat cgtactcacc agcgatttct atgcctacga caccaagtac attgacgaag
     3601 atggcgcgaa agtggtagtt ccggcagcca ttgcaccaga gatcaacgat aagatccggg
     3661 cgattgccgt tcaggcttat cagacgttgg gatgcgcagg catggcgcgt gtagacgtgt
     3721 ttttaacccc agagaacgaa gtggtgatca acgagatcaa cacactgcct ggcttcacca
     3781 acatcagtat gtatccgaag ctgtggcaag ccagcggtct gggttacacc gatctgatca
     3841 cacgtttgat tgagctggcg ctggagcgtc acgctgcgga taacgcattg aaaaccacaa
     3901 tgtaatattt taacgcccga taatattctt attgggcgtt ttcagtttct tcatcgcgac
     3961 gacgaataat caaccccgcc agccagaaac taatcaccca cgtcaccaga ccgacggcat
     4021 aggtctgcca accttttgct tcaaatccca gtacccccac cacgccatta agaatgaata
     4081 taaggccaat ggcgaacgca tagtagtgcc agtcacggcg gatttttaca ggcagattca
     4141 tggcagctcc gttaacgaaa aacaacatcc ttgcatattc tgtaacaacc gtctgtggtg
     4201 tagacaacat tttcgtaatt attaataaat ttaacttaag gttgccattt tgtgactcac
     4261 aacgaagatt cacgcttcgg gaatattccc aacatcaatt taccatgatt ctgatattga
     4321 caatctaaat gacctgtcag acttgccata agccgctggt atcacaccag aagagaagca
     4381 gatgacttcc ggaggttttt atggctgatt tcaccctgtc aaaatcgctg tttagcggaa
     4441 aatatcgcaa tgcctcttca acgcctggca acattgccta tgcgttgttt gtgctgtttt
     4501 gcttttgggc tggggcgcaa ttgctgaacc tgttagtgca tgcgcccggc gtctatgagc
     4561 gtttaatgca ggtccaggaa acaggtcgcc cacgggtgga aattggttta ggtgtcggca
     4621 ccattttcgg gctgatcccg tttttagcag gctgcctcat ttttgcagtg gtggcgctat
     4681 ggctgcactg gcgacatcgc cgccagtaaa atttacgttg ccagatgcgg cctgaaacgc
     4741 cttatctggc ctgacattcc tgtttgatca ctgtttcata caacgcgccc gccgttcgtc
     4801 tacgcgcttc gcaaaccagg ccgtagtcag gttgcgggtg attttcgggc tttccagttg
     4861 aatgccaggc aacatttcgc ggggtagtga tttacccgtt ttcgcttcgg caagttgata
     4921 aactttcttg tacagcgccg tctcctcaaa agagaagctg tcacctttct ctaactgacg
     4981 gcgaatttcg ctgtcgctca tccccagttt tcccgccagt ttgcgcgttg ccagttcggt
     5041 tttcccgggt tctttactgt cgtaacgaat taaatcaccg tccagtgcca gcttcacgcc
     5101 gctggcctta ctgactgcgt tctgaaacgc ggcattacgg ctggcgtacc agccagcgtt
     5161 aaaatcagca aagcggtata tcggtgcgct atagctggcg ggataattca gtaaatggta
     5221 agtaccaaac cacaacccgc cgcggcgact gaagacttcc tgacgaactg taccgtccat
     5281 tttccacgga taccctttgg tatgctgttc ggcaaaagca atgctgacct gcatcggccc
     5341 accggtgcgc accggattaa gcgaaccaaa cagcgtctgc cccattggta ccatgctgat
     5401 cagatcgtca aaaatcgcgc tcaattgctt ttctgtacgc accgaatcta accgttcgct
     5461 ataacttttg ccgtttggcg atttgatttt tagcgcggta tgaaccagaa aagcaggaat
     5521 gtgcatccgt tcagcacgac ggtcaatctc ttgccaggcg atcttgctta agcccggaac
     5581 ggccggatct gcctgataat ttgactcctg ctgcgccacc gccagcaccg agtagacgtt
     5641 ttccagcgtc ggtgccagcc cctgactttc aaaggtggtg gcgagatctt tcgcccaggc
     5701 atcgcggtct ttcacgctcg caggcatctt ctgccgcacg acgctcgcta catctatcgc
     5761 tttttcgccc tttttcaacg gttgcggagc ctgactacta caccccgcta aaaccagaac
     5821 cgccaacaat gacagcgaag atagatgatt gacgcgcgac attacttctc ctttttagct
     5881 caaggtatgg gttacttcct gaattttgtc accatccagc tcatgttcaa agctgcgcag
     5941 acgtttgtag atagacatca actcaaccag tgtggtccat gagttaatca ggtactggaa
     6001 agcaccgcga acctgaccaa aaacgttggt aatctgcgtc atcaggccga gcgtaatcgt
     6061 accggcaaca attgacggaa acagcaagaa caaaccgaaa acgttatcga cctgcaaata
     6121 gagaatgcgg gcgatgttga aatacatata gtgaaaatag aggcgaaaat agtttttccg
     6181 tacggcgcta aacagctcgc gtaccgtagg cggcgtcgcg cgcgtggcat cgtcttcacc
     6241 ataaaccagc tctttacggt aggcggcctc tactcgctgg tttttaaact ccagccccgg
     6301 cagtttgatc cctaccactg ccagcaatcc ggtccccatc agcgaccaga cgatagcggc
     6361 aatcaccaga ccatacggaa tgtgcccgat aatcggcagc tccggcacat gcgcggagag
     6421 cgttaccagc accggcagga aggcgatcaa cgtcatgatg gcgttgataa aactgacgcc
     6481 catattctcc agcgttgaag caaaacgcat ggtgtcttcc tgcacacgct gtgcggcacc
     6541 ttcgatatga cgcagttgtt gccagttcgc catgtaatat tcgttcatcg ccgtacgcca
     6601 gcggaacacg tagtgactga caaagaagtt gttcagtaca ctgatcacca ccgcgatcag
     6661 cgcaatcccc agaaagacgc ccacttcgcg gtaaaattgt tcgatagtga ctttatgcgg
     6721 cgaacttagc gcggtttgaa tcagatcata gaacggcgca taccaggcgt tgacggcgac
     6781 cccgacttcc accaaaaacc aagtgacgaa gataatcagc gcagtaccga gtattgacca
     6841 gtattgccaa cggtgcgggc tgtagataaa ccagaaaaat gcaaaaagtc ctacgcaaac
     6901 aatgtagtaa gcgtaaaaaa tcaggaaatc caacgaccag aaacgcgcgg cgctaatcgg
     6961 gatctgcccg gaagcgccgg tgatacgtgc cacccagtca cccccaccgg cttgccagaa
     7021 gataacggca atcaatgccc aaacaaaggc cgagagaaaa aacgttcccg gctttgggaa
     7081 aaaagactta aacatatgca ctcctgctaa cttcttatcg ttttaacaac tgctgtgtat
     7141 taaccgcatg accgctacaa agcgtcagcc aaatgaaacc gcccattgtg acccatagca
     7201 aggcgcttag ttcgccagcc tgaccataag aattgtttcg acggatttca caaaccaaag
     7261 attgtttaca taagcggatt aacacgcgca ggatcacaaa tgcaggcatt ggtgagcagg
     7321 aaaaagatcg tgtttacgcg ccgttacgtt tttaacacta ttattgcact aattcgccct
     7381 ttgcaatcca tcaatgagta gtataaatac gctcagttac cttcattcaa tctatggaca
     7441 ccaccgttga aacgtagtct gcttttttct gccgtgctgt gtgcggcgtc attgacctcc
     7501 gtccatgcgg cgcagccgat cactgaaccg gagtttgcct ctgatattgt cgatcgttat
     7561 gccgatcata ttttttacgg cagcggtgcc acggggatgg cgctggttgt tatcgacggt
     7621 aaccagcgcg tctttcgtag ttatggtgaa acgcgacctg gtaataacgt tcgcccgcag
     7681 ctggattcgg tcgtgcgtat tgcttccctc accaaactaa tgaccagtga aatgctggtg
     7741 aaattgctcg accaggggac cgtaaagtta aacgatccgc taagtaaata tgctccgcca
     7801 ggcgcacgcg tgccgaccta caatgggacg cccattaccc tagtgaatct ggcaacccat
     7861 accagcgccc tgccccgtga acagcccggt ggcgcggcac atcgtccggt gtttgtctgg
     7921 ccaacgcgcg agcaacgctg gaaatacctt tctacggcga agctgaaagc cgcgccaggc
     7981 agtcaggcgg cgtactctaa ccttgcgttc gacctgctgg cagatgcact ggcgaatgcg
     8041 tccggcaagc cttataccca gttgtttgaa gagcagatta cccatccatt ggggatgaaa
     8101 gacaccacct acaccccctc accggatcag tgccgccgtt taatggttgc tgagcgcggt
     8161 gccagtccgt gcaataacac gctggcggca attggcagcg gcggcgtcta ttccacgcct
     8221 ggcgatatga tgcgctggat gcagcagtat ttgtcatctg atttttatca gcgcagtaac
     8281 caggcagatc gcatgcagac gttgatttat cagcgcgcgc agtttacgaa agtgattggc
     8341 atggatgtcc ccggcaaagc ggatgcgctt ggtttaggct gggtatatat ggccccgaaa
     8401 gagggtcgtc cgaggattat tcagaagaca ggcggtggtg gcggtttcat tacctatatg
     8461 gcgatgatcc cgcagaagaa tattggcgcg tttgtggtgg tcactcgttc gccgttgacg
     8521 cgctttaaaa atatgagcga tggcatcaat gatctggtga ccgagctaag cgggaataaa
     8581 ccgttggtta tccccgcatc ctgattaata ctctgaaggc aaacggttga tagcgaccag
     8641 tttgccttta ttcatttcga tatagccgcc tttacgcaaa gcagcgagaa cttctgcgac
     8701 gacagaacgc gatatgcgtg tacgttgatg gatataattc atcacaccaa tacgtgatcg
     8761 caattcttca ttccagtcaa tcattgataa taatgttgcg cgggtttgtt tggctggtag
     8821 atgatatccc gtacaatttc cttctgatat taatttgtat ggtatatcgt ttttcattaa
     8881 accatcagcc agccccataa tataaggagc ctgggtaata ccgataagta cgttttcttc
     8941 tctacgcaga gagataactc cttctaaaat aacgaaagta tcttcgttat tgacatcact
     9001 ggaaaatata acttgctttt cattattaaa ctcgaagcgc gtaccgtatc tggacaaaca
     9061 tttatcgagc ttaccaaatt cctgaagagg tttaactaca gataacattt gcgcgtcctt
     9121 tgcagtaatg cccgtcaaat ccttgacggg cattatttag attaaattac cagtatttct
     9181 tcggagtgaa gaatattacc aagtatattt aacacccacg ttcgcggacc agtcttgatc
     9241 tacgtcacca ccaccgaggt agttagcatc ggtataggcg ctgaagttct tggtgaagct
     9301 gaactgagta cccagaccaa cacgtaccgc agacccttca gtaccgttat cgatggaatc
     9361 accgttcaca tcgttatcgt tgttagagtc gtcgtagacg taagccagtt tgaagtacgg
     9421 agtcagagcc tgatcttcgc tgtaggtgaa ggtataacct gcatctacac ccagttcata
     9481 acgcatgctg tcgtaaggct gaccgtcaac tttcatgtcg ttgctcagct ggtagtcatc
     9541 accagactgg aacagaccag aaatgctgcc gtaaggagtc acgtaaccag catcacccag
     9601 tttgaagtcg taaccggttt tcaaaccgaa gccccaagcg tcggagttgg tgctaccgtc
     9661 aacgtaagta ccgttgctca tggttgcaga caggtcgttg ttgaagtgag agtagctcaa
     9721 g
//
LOCUS       DAJTSF010000129         9715 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000129 DAJTSF010000000
VERSION     DAJTSF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.129"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..276
                     /locus_tag="OO335_003518"
     CDS             <1..276
                     /locus_tag="OO335_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6003759.1"
                     /translation="YRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFY
                     LDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            373..525
                     /gene="hokE"
                     /locus_tag="OO335_003519"
     CDS             373..525
                     /gene="hokE"
                     /locus_tag="OO335_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6003760.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            876..1028
                     /gene="hokE"
                     /locus_tag="OO335_003520"
     CDS             876..1028
                     /gene="hokE"
                     /locus_tag="OO335_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6003761.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(1150..1770)
                     /gene="entD"
                     /locus_tag="OO335_003521"
     CDS             complement(1150..1770)
                     /gene="entD"
                     /locus_tag="OO335_003521"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR6003762.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1945..4185)
                     /gene="fepA"
                     /locus_tag="OO335_003522"
     CDS             complement(1945..4185)
                     /gene="fepA"
                     /locus_tag="OO335_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR6003763.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4428..5630
                     /gene="fes"
                     /locus_tag="OO335_003523"
     CDS             4428..5630
                     /gene="fes"
                     /locus_tag="OO335_003523"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR6003764.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5633..5851
                     /gene="ybdZ"
                     /locus_tag="OO335_003524"
     CDS             5633..5851
                     /gene="ybdZ"
                     /locus_tag="OO335_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR6003765.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5848..9693
                     /gene="entF"
                     /locus_tag="OO335_003525"
     CDS             5848..9693
                     /gene="entF"
                     /locus_tag="OO335_003525"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR6003766.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2151 a         2522 c         2663 g         2379 t
ORIGIN      
        1 tatcgtatga gaagatatca aaatatcctt aaagaacatg gtattaaaca aagcatgtcc
       61 agaaaaggca attgtctgga taatgctgtg gtggagtgtt tctttggaac cttaaagtcg
      121 gagtgttttt atcttgatga gttcagtaat ataagcgaac tgaaggatgc tgttacggaa
      181 tatattgaat actataacag cagaagaatt agcctgaaat taaaaggtct gactccaatt
      241 gaatatcgga atcagaccta tatgcctcgt gtataactgt ccaacttttt ggggtcagta
      301 caccctgggg ctgccactcc aaaccagaca atttggatgg tagtcccttc ttcgcatgga
      361 ggtaatataa acatgctgac gaaatatgcc cttgtggcag tcatagtgct gtgtttgacg
      421 gtactgggat ttacgcttct ggccggagac tcgctgtgtg agtttacggt gaaggaacgt
      481 aatattgagt tcagggctgt tctcgcttac gaaccgaaga agtagccgtt gtgcggggag
      541 taatccccgc atatccggtt gtcaggtcag gatggtaagg cacctgcttt acactttcgc
      601 ccgcggtcag tgatggctgc gggcgatttc gttttaagag caatgatgag ttttgccgac
      661 agaagtccga aacaggactc actgtataaa taaacagcta ttttgctaag gaagggtaag
      721 ataacggcgg gtgcctgaag ctttccggtt tcaggttaac tctgaggttt ggaaagatga
      781 agccccaaga gatatttcta tcaatcctgg ggctgccact ccgaacctca acaactcgga
      841 tggtagtccc ttcttcgcat ggaggcaata taaacatgct gacgaaatat gcccttgtgg
      901 cagtcatagt gctgtgtttg acggtgctgg gatttacgct tctggtcgga gactcgctgt
      961 gtgagttcac cgtaaaggaa cgtaatattg agtttaaggc tgttcttgct tacgaaccga
     1021 agaagtagcc gttgtgcggg gagtaatccc cgcatatccg gttgtcaggt cagagtggta
     1081 aggcacctgc tttacacttt cgcccgcagt cagtgatggc tgcgggcgaa tcgtaccaga
     1141 tgttgtcaat taatcgtgtt ggcacagagt tatgactatt ttttctttta tctgccagtg
     1201 cacagcaaac atctcattct cacgatgaat gatgacctgc tgtttattcc agctaattat
     1261 ctgatagtcc agaaaacctg catcagtttg gatctcactt gccttaaatg cgctctcttt
     1321 ggcggaaaat gcaagtgtca gcgccagaga aaaggctaaa ccgcagtctg cgagtcgctc
     1381 gtgttctgct ggtgtaataa tgttgtctgt caattcggtc gctgtttgcg cagaaaaaat
     1441 ttcttcgata tcaacgccaa tcggttgacg ggaaaccacg gccaatgccg tagccccgca
     1501 gtggctaata ctgccgtata cctccgcagg ccacaccggt tggcgtagct cgccgattgc
     1561 gggcacacat ttatagccat attcccgcaa ggcataaaca gcagcgatcc gtccggctaa
     1621 atgctctgtt ttacgtttac gtccagcgtg ttgcagttgt gcgtagtgcg gcagccagag
     1681 taaatcctgc tcacaaaaat tcgccggatc gaactcaaca aaatgcagcg tatgtccggc
     1741 aaaggggagg gaggtatgcg tagttttcat atcgaccata ttcgagactg atgacaaacg
     1801 caaaactgcc tgatgcgcta cgcttatcag gcctacgtga tcctgcaata tattgaattg
     1861 gcaagatctt tgtaggccgg ataaggcgtt cacgccgcat ccggcgtgaa cgacgcgcac
     1921 tttgtcaaca atctgacgtt agcatcagaa gtgagtatta atgctcatat accacgtacg
     1981 tcccggctcg ttataggtat acgcgccggc accggcgata tagttggctc ctgccagatc
     2041 gcccgtggtc tgggcattac ctgcacgcca caaacgtttg tcgaacaggt tgtccacgcc
     2101 gccggtcaga ctgacattct tcgtcacatc ccaggtcgcg ctcagaccca caatgctgta
     2161 aggactgatt tctttggttt ccgggccaac cgctggctga cctttatagt tgtacttctt
     2221 cggctgctgc ttgccgtacc aggtgaaggt cgtttgcatc gacaaatctt cccgtgcctg
     2281 ccagctcagc gttgagttca acgtgtactc cgggatgatc gacaaacggt cgcccgtggt
     2341 tttgttttca ctcttcagca tataagtgat gttattggtc cacatcaccg tttcgctaac
     2401 tggtacgttt aacgatcctt ccagaccttc aaccactgct ttcggcacgt tatcccactg
     2461 atagagatca gtgccgactg cgttttgccc tacagccaca tagcccgctt caatcttatt
     2521 gcgataatcg ttacggaacc aggtgacgcc cgccagccag ccgtcgcgtt tgaactccag
     2581 accaatctct ttgttgatgc tggtttctac tttcaggtca tcgttacctt gcagatagca
     2641 gccgcccgcg ctggcatagc agccctgacc tttactgtag agaatgtagt tcgggttagt
     2701 ctggtatagg ctcggcgctt tataggcgcg ggcaatgcct attttcagcg tgaagtcatc
     2761 gcctaaacct tgcgatatgt tcagcgccgg gctccagtta ttgccgacaa tactgtgatg
     2821 atcgaaacgc agccccggcg ttacgatggt gctgtcagtc agctccatgt tgttttcggc
     2881 aaacagcgag aaaatttctg cttttgaata cgggctacgg tcggtggcac tcacgccatc
     2941 aatagcgcca ccggtattcg ttccggtcag tgcttgggtg ttggaactta agtccttcat
     3001 ccgttgctga ttccactccg tacccagcgt cagcgtctgg ttaacgagga aatcaatcgg
     3061 caggttaact tcgctgtgca gcatcacgtc atcgagatcg atatcgacga aatcctgtgt
     3121 cgctttttcg ttaaatttcc cttcggtacc gcccgccaga ccttccggaa tacgcgagtt
     3181 acgggtgtgt tcatactgca cccagttgct ggtggtcacg ccgttatccc agccaccgtt
     3241 ccaggtcagc gcgtagttct ggcgatacag gcggttggtt tcatcgccat atttcgagcg
     3301 agtgtaagcg tcagagttgg tgttctgggt atcgcccgca tacaggttac cctggcggct
     3361 gtaacccgct tccagttcca gcgattgcaa tggtgcgaag tcccagcgca ccacgccgtt
     3421 aatatcttta ttgatcaccc cttcgcgccc ggctggtaac gtcgtggcat acgttccggc
     3481 acgcgcggac tgatggccct ggttgatatc ccacgcgtca gcctgagttt tgtcgaggtt
     3541 gccatacaag cggaagctga attcgtcgcc cagcggaccg gtcaggctaa agttagtgcg
     3601 tttggtggca ccttcctctt tatgttctgg cgcattgaaa tatgcgtccc aggagccgtg
     3661 ccactcgccg ctgccttttt tggtaatgat gttaaccacg ccgcccgccg cgccgttgcc
     3721 ataacgcgca gctgccggac cacgcagaac ttcaatacgt tcaatcattt caggtggcac
     3781 ccaggaggta tcaccacggg tatcgcgctc gccacgccag ccctgacgca ccgagttacg
     3841 gctgcttacc ggcttgccgt caatcaaaat cagcgtgttt tccggaccca taccgcgaat
     3901 atcaatctgt cggttattcc cacgctgacc actggtggag ttaccggtca ggttaacgcc
     3961 tggcatggta cggatgatct ccgacacatc gcgggcaacc gggtttttgc ggatttcatc
     4021 tgcggtgatg gtcgaaacgc caggagcctg caagttctgc tcggcggcgg taacgacaat
     4081 ggtatcgtca tgtgaaacag gtgtatcggt cggctcttgt gcctgcgcta ccccataaat
     4141 ccccagattg accaacaagg ccagggaatg aatcttcttg ttcattgttt tattcctgca
     4201 tttttgccac gaattgcaac tgtcgggcat ggtcgtcatc aacacgacgc atcccgttac
     4261 cgcgaaaacc tttgatcctg aaagacacgc agtgcagttg gttaattaat gtccgcgctt
     4321 cccacggcgc gccattacgc tattgcaaat gcaaatagtt attaataaca ttatcaatat
     4381 atttctgcaa tcaatgaaaa attgcacagt aaacatgggg ttatggtgtg acggcgttaa
     4441 aagtaggaag tgagagctgg tggcagtcga aacatggccc ggaatggcag cgtctgaatg
     4501 acgaaatgtt tgaggtcact ttctggtggc gtgatcccca aggttctgaa gaatactcga
     4561 cgataaagcg cgtatgggtc tacatcactg gtgtgaccga tcatcatcag aacagccagc
     4621 cccagtcgat gcagcgaatt gcaggtactg atgtctggca gtggacgaca caactcaatg
     4681 ccaactggcg cggcagctac tgctttattc ccaccgaacg cgatgacatt ttttctgcac
     4741 catcccccga tcgcctcgaa ttacgtgaag gctggcgaaa actattaccc caggcaatag
     4801 ccgatccgct gaatccacaa agctggaaag gcgggctagg gcacgctgtt tctgcactcg
     4861 aaatgccgca agcgcctctg caaccgggat gggattgtcc gcaagcgcca gaaataccag
     4921 ccaaagaaat tatctggaaa agtgaacggt tgaaaaattc acgtcgtgta tggattttta
     4981 ccaccggcga tgtaacagca gaagaacgcc cgctggcagt tttgctcgat ggcgaatttt
     5041 gggcgcaaag tatgcccgtc tggcctgtgc tgacttcgct gacccatcgt cagcaacttc
     5101 ctcccgccgt gtatgtgttg atcgacgcta tcgacaccac gcaccgcgcc cacgaactgc
     5161 cgtgtaatgc ggatttctgg ctggcagtac agcaagagtt attacccctg gtgaaagtta
     5221 ttgccccttt tagcgatcgc gccgatcgca ccgtggtcgc cgggcagagt tttggtgggc
     5281 tgtccgcgct gtatgccgga ctgcactggc ctgaacgctt tggctgtgta ttaagccagt
     5341 caggatcgta ctggtggccg catcggggcg ggcagcaaga gggcgtgtta cttgaaaagc
     5401 taaaagctgg tgaagttagc gccgaaggtc tgcgcattgt gctggaagcg ggtattcgcg
     5461 agccgatgat catgcgggcc aatcaggcgc tgtatgcgca attacaccct ataaaagaat
     5521 ccattttctg gcgtcaggtt gacggcggac atgatgcgct ttgttggcgc ggtggcttga
     5581 tgcaggggtt aatcgacctc tggcaaccac ttttccatga caggagttga atatggcatt
     5641 cagtaatccc ttcgatgatc cacagggagc gttttacatt ttgcgcaatg cgcaggggca
     5701 attcagtctg tggccgcaac aatgcgcctt accggcaggc tgggacattg tgtgtcagcc
     5761 gcagtcacaa gagtcctgcc agcagtggct tgaagcccac tggcgtactc tgacaccggc
     5821 gaatttcacc cagttgcagg aggcacaatg agccagcatt tacctttggt cgccgcacag
     5881 cccggcatct ggatggcaga aaaactgtcg gatttaccct ccgcctggag cgtggcgcat
     5941 tacgttgagt taaccggaga ggttgatgcg ccattactgg cccgcgcggt ggttgccgga
     6001 ttagcgcaag cagatacttt gcggatgcgt tttacggaag ataacggcga agtctggcaa
     6061 tgggtcgatg atgcgctgat attcgaactg ccagaaatta tcgacctgcg aaccaatatt
     6121 gatccgcacg gtactgcgca ggcattaatg ctggcggatt tgcaacaaga tttgcgcgtc
     6181 gatagcggta aaccactggt ctttcatcaa ctgatacagg tggcagataa ccgctggtac
     6241 tggtatcagc gttatcacca tttactggtc gatggcttca gttttccggc cattacccgc
     6301 cagatcgcca acatttactg cgcattgctg cgtggcgaac caacgcctgc ttcgccgttt
     6361 acgcctttcg ctgatgtagt ggaagagtac cagcaatacc gcgaaagcga agcctggcag
     6421 cgtgatgcgg cattctgggt ggaacagcgt cgccaactgc caccgcccgc gtcactttct
     6481 ccggcacctt tagcggggcg cagcgcctcg gcagatattt tgcgtctgaa aatggaattt
     6541 accgacgggg aattccgcca gctggctacg caactttcag gtgtgcagcg taccgattta
     6601 gcccttgcgc tgacggcttt gtggctgggg cgattgtgca atcgcatgga ctacgccgcc
     6661 ggatttatct ttatgcgtcg actgggctcg gcggcgctga cggctaccgg acccgtgctc
     6721 aacgttttgc cgctgggtat tcatattgcg gcgcaagaaa cgctgccgga actggcaacc
     6781 cgactggcaa cccgactggc agcacaactg aaaaaaatgc gtcgtcatca acgttacgat
     6841 gccgaacaaa ttgtccgtga cagcgggcga gcggcaggtg atgaaccgct gtttggtccg
     6901 gtactcaata ttaaggtatt tgattaccaa ctggatattc ctggtgttca ggcgcaaacc
     6961 cataccctgg caaccggtcc ggttaatgac cttgaactgg ccctgttccc ggatgaacac
     7021 ggtgatttga gtattgagat cctcgccaat aaacagcgtt acgatgagcc aacgttaatc
     7081 cagcatgctg aacgcctgaa aatgctgatt gcccagttcg ccgcggatcc ggcgctgttg
     7141 tgcggcgatg tcgatattat gctgccaggt gagtatgcgc agctggcgca gatcaacgcc
     7201 actcaggttg agattccaga aaccacgctg agcgcgctgg tggcagaaca agcggcaaaa
     7261 acaccggatg ctccggcgct ggcagatgcg cgttacctgt tcagctatcg ggaaatgcac
     7321 gagcaggtgg tggcgctggc gaatctgctg cgtgagcgcg gcgttaaacc aggggacagc
     7381 gtggcggtgg cactaccgcg ctcggtcttt ttgaccctgg cactccatgc aatagttgaa
     7441 gctggcgcgg cctggctacc gctggatacc ggctatccgg acgatcgcct gaaaatgatg
     7501 ctggaagatg cgcgtccgtc gctgttaatt accaccgacg atcaactgcc gcgctttagc
     7561 gatgttccca atttaaccaa cctttgctat aacgccccgc ttacccccca gggcagtgcg
     7621 ccgctgcaac tttcacaacc gcaccacacg gcttatatca tctttacctc tggctccacc
     7681 ggcaggccga aaggggtaat ggtcgggcag acggctatcg tcaaccgcct gttgtggatt
     7741 caaaatcatt atccgctcac aggcgaagat gtcgttgccc aaaaaacgcc gtgcagtttt
     7801 gatgtctcgg tgtgggagtt tttctggccg tttattgccg gggctaaact ggtgatggct
     7861 gaaccggaag cgcaccgcga cccgctcgct atgcagcaat tctttgccga atatggcgta
     7921 acgaccacgc actttgtgcc gtcgatgctg gcagcatttg ttgcctcgct gactccgcaa
     7981 accgctcgcc agagttgcgt gacgttgaaa caggttttct gtagtggtga ggccttaccg
     8041 gctgatttat gccgcgaatg gcaacagtta accggcgcgc cgttgcataa cctgtatggc
     8101 ccgacggaag cggcggtaga tgtcagctgg tatccggctt ttggcgagga actggcacag
     8161 gtgcgcggca gcagtgtgcc gattggttat ccggtatgga atacgggtct gcgtattctt
     8221 gatgcgatga tgcatccggt gccgccgggt gtggcgggtg atctctatct caccggtatt
     8281 caactggcgc aggggtatct tggacgaccc gatctgaccg ccagccgctt tattgccgat
     8341 ccttttgctc ctggtgaacg gatgtatcgt accggagacg ttgcccgctg gctggataac
     8401 ggcgcggtgg agtacctcgg gcgcagtgac gatcagctaa aaattcgcgg gcagcgtatc
     8461 gaactgggcg aaatcgatcg cgtgatgcag gcgctgccgg atgtcaaaca agccgttacc
     8521 cacgcctgtg tgattaacca ggcggcagcc actggtggtg atgcgcgtca gttggtgggc
     8581 tatctggtat cgcaatcggg cctgccgttg gataccagcg cattgcaggc gcagcttcgc
     8641 gaaacattgc cgccgcatat ggtgccagtc gttctgctgc aactgccgca gttgccactt
     8701 agcgccaacg gcaagctgga tcgcaaagcc ttaccgttgc ctgaactgaa ggcacaagcg
     8761 ccagggcgtg cgccgaaagc gggcagtgaa acgattatcg ccgcggcatt cgcgtcgttg
     8821 ctgggttgtg acgtgcagga tgccgatgct gatttctttg cgctgggcgg tcattcgcta
     8881 ctggcaatga aactggcagc gcagttaagt cgacagtttg ctcgtcaggt gacgccgggg
     8941 caggtgatgg tcgcgtcaac cgtcgccaaa ctggcaacga ttattgatgg tgaagaggac
     9001 agctcccggc gcatgggatt cgaaaccatt ctgccgttgc gtgaaggtaa tggcccgacg
     9061 ctgttttgtt tccatccggc gtccggtttt gcctggcagt ttagcgtgct ctcgcgttat
     9121 ctcgatccac aatggtcgat tattggcatt cagtcgccgc gccctcatgg acccatgcag
     9181 acagcgacaa acctggatga agtctgcgaa gcgcatctgg caacgctact tgagcaacaa
     9241 ccgcacggca ttgcggcgcg gctgcgtgcc cgtggcgaac aggtggcatt tcttggtttg
     9301 ctggatacct ggccgccaga gacgcaaaac tggcaggaaa aagaggctaa tggtctggac
     9361 ccggaagtgc tggcggagat taaccgcgaa cgcgaggcct tcctggcggc acagcaggga
     9421 agtacttcaa cggagttgtt taccaccatt gaaggcaact acgctgatgc tgtgcgcctg
     9481 ctgacgactg ctcatagcgt accgtttgat ggtaaagcga cgctgtttgt tgctgaacgc
     9541 acgcttcagg aaggtatgag ccccgaacgc gcctggtcgc cgtggattgc ggagctggat
     9601 atctatcgtc aggattgtgc gcatgtggat attatctctc caggggcgtt tgtaaaaatt
     9661 gggccgatta ttcgcgcaac gctaaacagg taaattaata ttatctataa accca
//
LOCUS       DAJTSF010000130         9681 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000130 DAJTSF010000000
VERSION     DAJTSF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.130"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(26..214)
                     /locus_tag="OO335_003526"
     CDS             complement(26..214)
                     /locus_tag="OO335_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6003767.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO335_003527"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO335_003527"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6003768.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="OO335_003528"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="OO335_003528"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6003769.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="OO335_003529"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="OO335_003529"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6003770.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="OO335_003530"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="OO335_003530"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6003771.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="OO335_003531"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="OO335_003531"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6003772.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6242
                     /locus_tag="OO335_003532"
     CDS             5109..6242
                     /locus_tag="OO335_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6003773.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6331..6864
                     /gene="blc"
                     /locus_tag="OO335_003533"
     CDS             6331..6864
                     /gene="blc"
                     /locus_tag="OO335_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6003774.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="OO335_003534"
     CDS             complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="OO335_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6003775.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="OO335_003535"
     CDS             complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="OO335_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6003776.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="OO335_003536"
     CDS             complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="OO335_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6003777.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7788..8354)
                     /gene="efp"
                     /locus_tag="OO335_003537"
     CDS             complement(7788..8354)
                     /gene="efp"
                     /locus_tag="OO335_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6003778.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8396..9424
                     /gene="epmB"
                     /locus_tag="OO335_003538"
     CDS             8396..9424
                     /gene="epmB"
                     /locus_tag="OO335_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6003779.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2480 c         2466 g         2270 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg
     5881 atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa
     5941 ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca
     6001 gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta
     6061 agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
     6121 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
     6181 aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt
     6241 aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac
     6301 acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg
     6361 acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta
     6421 gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat
     6481 caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc
     6541 ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa
     6601 gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt
     6661 cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt
     6721 tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa
     6781 gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt
     6841 tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg
     6901 attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg
     6961 gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt
     7021 aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata
     7081 acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact
     7141 tccagcagac cagcaataac taagataatc caggacatat caggctccgg aaatttgggg
     7201 ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat
     7261 attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca
     7321 aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc
     7381 agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc
     7441 agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat
     7501 aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa
     7561 catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca
     7621 gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta
     7681 cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct
     7741 gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga
     7801 gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc
     7861 ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta
     7921 tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta
     7981 acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat
     8041 ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc
     8101 atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca
     8161 gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga
     8221 cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct
     8281 tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta
     8341 tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc
     8401 gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt
     8461 tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt
     8521 ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg
     8581 catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga
     8641 gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc
     8701 tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt
     8761 aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg
     8821 taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt
     8881 ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact
     8941 ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc
     9001 ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct
     9061 ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa
     9121 attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga
     9181 taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta
     9241 ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga
     9301 agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact
     9361 ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca
     9421 gtaactgatt aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg
     9481 cctacatcgt gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt
     9541 tacgccgcat ccggcaagag caacaaacgc aagtacagca accactgaga aatttaatct
     9601 ttgcacgttg cataaccttt aattattcat atgcattgct gttgttactt tttatatagc
     9661 taacaccaat aattaacctt t
//
LOCUS       DAJTSF010000131         9520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000131 DAJTSF010000000
VERSION     DAJTSF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.131"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..591)
                     /locus_tag="OO335_003539"
     CDS             complement(<1..591)
                     /locus_tag="OO335_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR6003780.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(658..1383)
                     /gene="fimC"
                     /locus_tag="OO335_003540"
     CDS             complement(658..1383)
                     /gene="fimC"
                     /locus_tag="OO335_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6003781.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1420..1959)
                     /locus_tag="OO335_003541"
     CDS             complement(1420..1959)
                     /locus_tag="OO335_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6003782.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2024..2572)
                     /gene="fimA"
                     /locus_tag="OO335_003542"
     CDS             complement(2024..2572)
                     /gene="fimA"
                     /locus_tag="OO335_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6003783.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3053..3649)
                     /gene="fimE"
                     /locus_tag="OO335_003543"
     CDS             complement(3053..3649)
                     /gene="fimE"
                     /locus_tag="OO335_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR6003784.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4127..4729)
                     /gene="fimB"
                     /locus_tag="OO335_003544"
                     /pseudo
     CDS             complement(4127..4729)
                     /gene="fimB"
                     /locus_tag="OO335_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            6183..6899
                     /gene="nanC"
                     /locus_tag="OO335_003545"
     CDS             6183..6899
                     /gene="nanC"
                     /locus_tag="OO335_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6003785.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6919..8025
                     /gene="nanM"
                     /locus_tag="OO335_003546"
     CDS             6919..8025
                     /gene="nanM"
                     /locus_tag="OO335_003546"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6003786.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8090..9070
                     /locus_tag="OO335_003547"
     CDS             8090..9070
                     /locus_tag="OO335_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6003787.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2787 a         2077 c         1996 g         2660 t
ORIGIN      
        1 ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac cgggatccca
       61 taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag ggatcgtcag
      121 gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcctggacca ttgtggttaa
      181 tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg tattcagccc
      241 catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac tgtcgcccgt
      301 attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat cgacgcgata
      361 cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca cagcctgggg
      421 atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag gtgcctgtgc
      481 ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat gcttacgaat
      541 atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca ttattcctcc
      601 ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc ccctgcgtta
      661 ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg ttcggtaagt
      721 aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg gaggcaccaa
      781 tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt aatagggtgt
      841 cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt ctgcggcctg
      901 atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc tgataattgc
      961 gagctgtagc atattctcag tcaattttga tttatccatt gacggaatcg ctttaacgtt
     1021 catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat caagaatacg
     1081 taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga taaaacgacc
     1141 atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac tattttcatc
     1201 attatttgtc acggcaagtt gcacttgttt ttgccctgcc ggataaatta cgcgagtcgc
     1261 acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga acatcaggat
     1321 acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt ttttattact
     1381 cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg ttaaagagaa
     1441 ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac gatatttggc
     1501 gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg gaggacgatt
     1561 aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg aggctatccc
     1621 tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac cgtgaaacgc
     1681 cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa caaaaggcac
     1741 cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc ccatattgac
     1801 cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga cgccctgaaa
     1861 ttgcatattt ccgccgggca gcgtggtatt ccatttattt ccggccagag caaacattgg
     1921 cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta tcgcacaagg
     1981 gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga tactgaacct
     2041 tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca taataacgcg
     2101 cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta aatgtcgcac
     2161 cgtccagcgt cagagcattg cctgttctgt ctaggatctg cacgccaacg tttgttgcac
     2221 tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca attgccgtac
     2281 ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc agctgaatgt
     2341 taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta cgaacctgtc
     2401 ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg ttaacaactt
     2461 cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga gccgctgcgg
     2521 aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc atgctgcttt
     2581 cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact ttgttttgtc
     2641 aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca tttggggcca
     2701 ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg ttggcttaat
     2761 attctattat tatctttatt tatagatttt tatattgcat gaggtggttt ttggagagaa
     2821 gaatgaggaa gatgcgtcga gccacagaaa cgttagcttt acatatagcg gaggtgatgt
     2881 gaaattaatt tacaagagaa ataatttaca tatcaaatag ttagatgctt tttgtcgttt
     2941 tttaatattt ttatgcttga gaaaaaatac gtaacttatt tatgatatgg acagtttggc
     3001 cccaattgtc ttgtgtttat ttgtttttta actttattat caataagtta aatcaaacct
     3061 cttctctttt taatttttcg tttatgagat tatttctttc ccataatccg gcaaaacgag
     3121 cagcattact ggcggtataa cgcacagtat ggcgaatatt tcgatgcccg agataatcct
     3181 gaattaaacg ggtatctgca ccacgctccg ccagttcata accgcaagca tgccttaaca
     3241 tatgaggatg agtctgcgtt acggttccag cttcaatacc ggcatcgcga ataatgcgat
     3301 aggcctgctg gcgagaaagc cgactcccgc ggcgagaaat aaatatagcg tcggtccggt
     3361 cagcgccttt ccagttagca cgttcctggg tccagcgttc cacggcttca cgctcatcaa
     3421 aacgtaacgg gtgaacggta gaaaatccgt tcttcagtcg gcgaatattt attcgacctt
     3481 cattaaggtc aaggtcctga taatgcagat caagcagttc actaatacgc atcccatgcc
     3541 gatatgccaa cagaataaga caataatctc tggctcccgt tgccccgtaa caaaccgcct
     3601 gcatcatggc ctgaacttct ttaccggtaa gataacgacg tttactcaca atagtagtac
     3661 tcctaactga gatatatttg aatgaatacc tataggaaac ctcaatcggt caaaattagc
     3721 ccaatagata aaaagataac cacacacgaa gttatgtggc tatcagtcaa ttacattaaa
     3781 catcatacaa aaataaaata tcactgatgt gacaacatct attttcattg atttgattca
     3841 atataacaat agattacaga ggattaactt ccagttactt aaaaaataaa gaaaaaagtg
     3901 tggaaacagg agaatttaag tgatgtgtaa acttatgaat gattttgttt atttattttg
     3961 aagaacataa atcattagat tttgtatggg tatggttaac ttaatgttta ttatgataat
     4021 taagaatcac acttacaata acatcaatta ataaatggtt aatcattttt gatatatcgt
     4081 aagaataatg tagtttttaa caccatccct ggtatctcaa ctatctctat aaaacagcat
     4141 gacgctgtcg tcctctggct ctatcccaga tgccgtaaaa acgccctgca ttgctggcgg
     4201 tataccagac agtatgacga atattgcgat gcccaagata atcctagata agtcgcgtat
     4261 ctattcccat attcgccaaa gcaaaaccac acgaatagcg taacatgtgc ggatgaatct
     4321 ccagtgacaa cccggcatta ccaccagaag tcgagataat atggtaaaac tgttgccgag
     4381 aaagcggatt ccccttacgt gataaaaata cccactcgct ctcagcatgc ggataagaag
     4441 tacggatact caaccagttt tttaaagcct gaacttcttt gttcaatagc gggtgcgttg
     4501 ttgaaaagcc tttttttaat cgatggatat atatacactt tgctttaaga tcaatatccg
     4561 aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa agcaaaatca
     4621 gacaataatt acgtgttgca tgaggcccgg tatttgctgc tttaaggagt gactcgattt
     4681 cactatgggt aaggaagttc ctttttttgt tatcagcctt attcctcatc gttttccctt
     4741 ataattacag acgcgcacta gctgtgctgg gttagcaata atccaacatt tttatcctga
     4801 ttatgtttat aaaagcgaac gtttgcttaa taacttacgt aagtggacca gttcttctga
     4861 gcgaacttaa atggagtagc gatagttact tataataact ttgccataca aacgccatgc
     4921 tcttgcatgc tatgtacctt tatatattta tcaatctgat acgaatctaa taataaccct
     4981 catcattaat gcaactaatc ttatctatat atcatgtgat atgttctgta acaagtaaaa
     5041 tcaacataaa aaattcagca aaatttaata tttgttaaac tagccgtctc aaatatgaca
     5101 ttattagtta actgcatttg ccacaaaatg gctatttcat ggaacgcagt aatttcttag
     5161 tggctattat catgctaatt cttagatccc tagcgattat tcctgcctgg ttcgtcagct
     5221 taggccatga gattgttaca ttccttttta tcctggagat atgggggaac gcaggcgacg
     5281 ctttttcatc tacatccgtt ggtaaatata gctttggtca ctacgactta agccttcctc
     5341 aatcacagct attcgccggg cgatacggca aacaaactaa gtgaaacatc atcctgaaaa
     5401 caccaacatc aacaagcctc tccagatcga catcaggagt gaccagttac aagccacaaa
     5461 ccaagaaaca tatttcatga tgagaattat gctcagcctg atggcgagta aacccttatt
     5521 ttcaccactc tcactgagtt agcttgcaaa gctttctttg tttagattct aaataaatta
     5581 tttataaaac gtatagttaa aagaaaatct ttagtttttg aagaccaccg caagtgttcg
     5641 tctggcttca catggcatct tcttcttaga aaaagatcga catattttgt gacgcgaatt
     5701 gcaaatctgg ttttgttgta tggattgcgt gatttttgat ctggtataac aggtataaag
     5761 atgcaccacg atagtcaatg agactgggca tctcgtaatc tatggcaaac atcacttcag
     5821 ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa aggaatatga aaatgagatc
     5881 aacagacact ctattttatg actctgggta aaatgggttg agtaagtgat ataacttacg
     5941 aatattcaaa tcaattaaaa atcagaagag attttatact caggtattta atctggatct
     6001 ctgtttattt aaataatgtt aaaagagaat tttcacagga gaccttatac aaaaaatata
     6061 aaatacagtt acccgttgcc aaagacacta taagcctggc aaaaaaatat tacacaacat
     6121 aaatgctaat tgtttatgcg ggctttgtat tgctttctgt atcctacaaa tgagtgaaat
     6181 ttatgaaaaa ggctaaaata ctttctggcg tattattact gtgcttttcg tccccattaa
     6241 tttctcaggc tgcgacactg gacgtacgtg gtggatatcg tagtggaagc cacgcctatg
     6301 agactcgact caaagtcagt gagggatggc aaaatggatg gtgggcaagc atggaaagta
     6361 atacctggaa taccattcat gataataaaa aggaaaatgc cgcactcaat gatgttcagg
     6421 ttgaagttaa ttacgcaatt aaacttgatg atcaatggac ggtgcgcccg ggaatgttaa
     6481 cgcattttag cagcaacggc acacgctacg gaccctacgt aaaactgtcc tgggacgcga
     6541 caaaagatct taattttggc attcgctatc gttacgactg gaaagcttac cgacaacaag
     6601 acttatccgg tgatatgtct cgtgataacg ttcatcgttg ggatggatat gtcacttacc
     6661 atattaatag tgatttcacc ttcgcatggc aaacgacgct atacagcaaa cagaatgatt
     6721 atcgctatgc aaaccacaag aaatgggcga cggaaaatgc ctttgttcta caataccata
     6781 tgacgcccga tattacgcca tacatagaat atgactacct tgaccgccag ggtgtttaca
     6841 acggcagaga taatttatcg gaaaacagtt atcgcattgg tgtgtcattt aaactgtagt
     6901 agacaggaga cagtcacaat gaataaaact ataatggcgc ttgctatcat gatggcttca
     6961 tttgccgcaa acgcgtctgt attaccggaa actcctgtgc catttaaaag tggtaccgga
     7021 gcaattgata acgacactgt ctacattggt ttaggtagcg caggtacagc atggtacaag
     7081 ctagatacac aggccaaaga taaaaaatgg actgcgttag ctgcattccc tggtggaccg
     7141 agagagcaag caacctctgc atttattgat ggcaatctgt atgtgtttgg cggcattggc
     7201 aaaaacagcg agggattgac tcaggtattt aatgacgtac acaaatacaa ccccaaaacc
     7261 aatagctggg ttaaattgat gtcgcacgcg ccgatgggca tggcgggtca tgtgactttt
     7321 gtacacaacg gcaaggctta tgttactggc ggtgttaacc agaatatctt caatggctat
     7381 tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta tagataaaat caacgcccac
     7441 tattttgaca aaaaagcaga agattatttc ttcaataagt ttctgttgtc ttttgatccc
     7501 tcaacacagc aatggagtta cgctggcgaa tcgccctggt acggaacggc tggtgcggcg
     7561 gttgtgaata aaggtgataa aacctggctt attaatggcg aagccaaacc aggattgcga
     7621 acggatgccg tatttgaact tgatttcacc ggtaataatt taaaatggaa taagcttgat
     7681 cccgtctcat caccagatgg cgtcgctggc ggttttgcgg ggataagcaa tgattctctt
     7741 atatttgctg gaggggccgg attcaaaggt tcacgagaaa attaccagaa cggtaagaac
     7801 tatgcgcatg aaggcctgaa aaaatcatat agcactgata ttcatctttg gcataacggg
     7861 aaatgggata aatcgggtga attatcgcaa ggtcgggcct acggagtatc attgccctgg
     7921 aataatagtt tattaattat tggcggtgaa actgcaggcg gcaaagcggt gacggattca
     7981 gttttaattt ctgtgaagga taataaagtc acagtacaaa actaacgctt cagggccccg
     8041 gtaaaggggc cctgctatca atttgtcatt tgtattacgc gaaaattata tgaacgcaat
     8101 aatatcaccc gattattact atgttcttac cgttgctggt cagtctaatg ccatggcgta
     8161 tggcgaagga ctgccattac cggacaggga agatgcgcct cattccagaa ttaaacaatt
     8221 agcgagattt gcgcatacgc atcccggagg cccgccatgt cactttaacg acattattcc
     8281 actgactcac tgcccacacg atgttcagga tatgcagggt tatcaccatc ctctggcaac
     8341 gaatcatcaa acacagtacg gcaccgttgg ccaggcactg catattgcac ggaaattact
     8401 gcccttcatt cctgataatg cagggattct tatcgttccg tgttgccgtg gcggatcggc
     8461 ttttaccgcg ggcagcgaag ggacatattc agaacggcac ggagccagcc atgatgcttg
     8521 tcgttgggga acggatactc cgctgtacca ggatttagtc agcagaacgc gagccgcact
     8581 ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg atgcaaggcg aatttgactt
     8641 aatgaccagt gactacgcgt cacaccctca acactttaat catatggttg aagcctttcg
     8701 tagggatcta aaacaatacc attctcagct taataatatt actgacgcgc cgtggttttg
     8761 cggcgatacc acctggtact ggaaagaaaa tttcccgcat tcgtatgaag ctatttatgg
     8821 caattatcaa aataatgttt tagccaatat tattttcgtc gacttccagc aacaaggtga
     8881 aagaggactg acgaacgcgc ctgatgaaga cccggacgat ttaagcacgg gatattacgg
     8941 ttcagcgtac cggtcaccgg agaactggac gacggcactg cgaagcagtc atttcagcac
     9001 ggcagcccgt cgggggatta tttctgacag atttgtagaa gcaattttgc agttttggcg
     9061 cgaaaggtga gcggggagtt tattcatggg ggatgtcctt gttcgttctc agagtagaca
     9121 acaaaaaaca aatgagatca aggtaatatc tcttcacttg cgggctcgaa cagcaggcaa
     9181 ttgccaatat aataatctgg cacaagggtg aaaaacgcgc accgcataaa ccgttgttat
     9241 tgcttacaga ttagccggat gccttaatag acatccgcgc cttttcgatt atggctcgga
     9301 aatctacgaa catctacaca gtttactgga acgttttggg ccacagcgtt cgcagcaccg
     9361 tcctgatatg ccgttctggc gattataagt cgacggattc tggcaaatag acgatgatga
     9421 tatgcttaat gatgttcaaa gaaattaggc cattattata ttattgcttg cattttcttt
     9481 cattcctaaa agcacccgcc attaatccca ggcgcattat
//
LOCUS       DAJTSF010000132         9495 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000132 DAJTSF010000000
VERSION     DAJTSF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.132"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(54..533)
                     /gene="xdhC"
                     /locus_tag="OO335_003548"
     CDS             complement(54..533)
                     /gene="xdhC"
                     /locus_tag="OO335_003548"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR6003788.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="OO335_003549"
     CDS             complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="OO335_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR6003789.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="OO335_003550"
                     /pseudo
     CDS             complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="OO335_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4131..4886
                     /locus_tag="OO335_003551"
     CDS             4131..4886
                     /locus_tag="OO335_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6003790.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4965..5038
                     /locus_tag="OO335_003552"
     tRNA            4965..5038
                     /locus_tag="OO335_003552"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4997..4999,aa:Gly,seq:ccc)
     gene            5173..6783
                     /locus_tag="OO335_003553"
     CDS             5173..6783
                     /locus_tag="OO335_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6003791.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6815..7422
                     /locus_tag="OO335_003554"
                     /pseudo
     CDS             6815..7422
                     /locus_tag="OO335_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8353..9099
                     /locus_tag="OO335_003555"
     CDS             8353..9099
                     /locus_tag="OO335_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6003792.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
BASE COUNT         2424 a         2347 c         2413 g         2311 t
ORIGIN      
        1 tgttaaacac ataacctcag tgaatccggc ctgaattcag gccggatatc cttttacttc
       61 gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca gcgacaaaga
      121 tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg tggtttcgcc
      181 agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg cactgtgccg
      241 gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc accttccagc
      301 gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc tattgctgtg
      361 ccgtcgacca acaccgtaca ggcaccacat tcacccacgc agcacccttg tttgacactt
      421 agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc gtgaagctga
      481 aaaggcatcc cgttaatggt gcattcggtg gtaattgttt cgctgtgatt cattgcaatt
      541 ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc tggatgagat
      601 gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct tgcaggacag
      661 attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg
      721 catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt aattcgctga
      781 aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata tccattgcgt
      841 cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat
      901 gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc
      961 cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc gcgtcataaa
     1021 ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg
     1081 tagcgacgtt acggatctgc ggtccagcaa tgaacgtggc cgcagcacat aacgccggga
     1141 gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca gagccgatac
     1201 gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta tgaatatcaa
     1261 caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg
     1321 cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca agggttgctg
     1381 cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc tgccagatgg
     1441 aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac accggtagcc
     1501 atcttcaccg cgttacgaat agcagcggca acaggaatta tgggtggctc acccagtgac
     1561 ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc cagttgtggc
     1621 agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac cacgccgctt
     1681 ttagcatcga tgatcatctc ttcaaatagc gcccagccaa tgcccattcc cattccgccg
     1741 tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg aacgttgagg
     1801 atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac aaaggtacag
     1861 ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag ctgcccgccg
     1921 cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa cggctcttcc
     1981 ggtcgttcaa ccagcacgat atggcctttt atcagggtca aattcatcgc tgactgatgt
     2041 agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc actgcgcagc
     2101 gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg
     2161 tcggtatctt gtgttgaaat aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc
     2221 accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg
     2281 ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac gccgacaggc
     2341 caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa attgccttgc
     2401 tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc tttttcaaga
     2461 cactccggca accctgcgct gcaaatacgt ttgcccgtga gcggattagc atctccttcg
     2521 cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca
     2581 tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg catcgcacca
     2641 gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca acgaggataa
     2701 aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga tgcataagcg
     2761 ccggtgttag acagaacatc cagactataa cctttcaagg ttccgtcgcg gttcacgccc
     2821 atttgcccgt caatagtaaa agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg
     2881 ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc cgccattggc
     2941 tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt gatgactcgt
     3001 acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc
     3061 tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag cgatgttacg
     3121 ctttccatgt gacaatgttg aataacggga gtctgatagt gcccctgtac ctggtagtcg
     3181 gcggcatcga ttgtttgttg gacattgccc gtcgacatcg tgctttgttt cagtaaattg
     3241 ccgccgttat ggattggtgc agcgtcttct gtcagcgccg cttctggcgt ggtgataacg
     3301 ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt gagttcatcg
     3361 cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg
     3421 cgatcggcgg tatcgcgctt gttttcgtca agtgtctagg catgcccagc tgtagcgaat
     3481 ggaatatcag gcacatcttc ccaagtaaaa atcgccagta cgcccggtaa acttctggct
     3541 tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac atatttcgcg
     3601 taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt gaccttagca
     3661 atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc ttccatagaa
     3721 atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc aggcaagatt
     3781 tacgccagaa acgacgataa gcgttagggg aggaaggaag tgtgatgacg ttaaaaaaac
     3841 acagatatga tacgaaacag gactctgaat agggacagtg atacgtatca atatgagtgg
     3901 cgataaaatc aatgtgataa atcaggatta ttacgcatca ggatgggaaa tagggtaaaa
     3961 agtcaggaac ttcgatacct ctacagcgaa tattttgctg atgatacttc cctttgccaa
     4021 atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat tgggtatcaa
     4081 gaggctggct gctatgatat ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg
     4141 gacgcctgaa taaaaaatct ctgggtatcg tgatgttttt atcggttgga ctgctattgg
     4201 cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc gtttacaccg
     4261 tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagagc
     4321 tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag aaactaaaac
     4381 tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgactaaaa
     4441 ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac
     4501 gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg
     4561 gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc gcgggtgcag
     4621 gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac
     4681 acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc
     4741 aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg gcatctgttc
     4801 gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc
     4861 cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgcggcact
     4921 tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat
     4981 ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc ccgctccatg
     5041 acacttctta aagtctacta acttactgaa tcctaaggga tacggcttaa cgccttccga
     5101 tagttttact cactgtaacc aacactgtga ctaactttgt tgaagtagtg agcagaccta
     5161 aggtctcagt acatgctcaa gtcaagaacc tacctttatc agcgtaatgg tgtcttttac
     5221 atccgtctta ggatgaagac cactagtcgc ctgaccgctt cgcttccttc tcacaaccgt
     5281 tataaactag cgtcagtatc tttacggacc aaggatagac gtaccgctat ggctcactca
     5341 cggcacatca agtcagcact taaagcaatc catgcagaca accctaacgc ctcttatgag
     5401 gagctacggg agcacctgaa gaccattgtc gagtgggaac ttagtgtcag ccgtgatgac
     5461 ctgaacgacc cggagtccta ccagctttac gttgaccagt acgatgacat taagtccaac
     5521 cttcgggaag ctgtagcgac cgagcgtctg actgtagacc agcatcgcta tattaatgac
     5581 gttatcggtg tgcttaaggc ctgtcaggat agactcaatg gcgatagctc tggcctattg
     5641 tcttaccttg agcctgagac tggcagtcta agaccgtccg tctccctatc tgtattggcg
     5701 gaacctgaag tccctgaacc taaagcactg accctcgcgt ctctcattga gcagtacgag
     5761 caggagaacg cccagaactg gaagccagcg accctaagcg aaaaccgagc gtcacactct
     5821 acgctaatcg agatattcga ctatctggat attcaggatg tcggcaaggc gacccgtgca
     5881 gatatgctca gagttcgtga agtcctccag cagctaccga agaaccgcaa gcaacgcttt
     5941 aagtctatgc cgctgtctga cttgctgaac cgggagtcta agactgactg tctggacgtc
     6001 gttaccatta acaataaata tcttatcaag atggctgctg tgtttaagtg ggcggtacgc
     6061 aacgacttga tagctaagaa cctgaccgaa ggtcttgagc tgaaagtccc tcagcgtaaa
     6121 gcctccgacg ctcgtgatgc gttcagtcct gagcaggtag ggcaactact ggtcgcagct
     6181 aaagcgtact cgcagaagac ctcaggtaag ccataccatt attacgtcac cgctctggct
     6241 gcgattacag gtgcgagact taacgaggtg gcccaacttc aggttaaaga tgtcaggacc
     6301 actgaggctg ggaccgtttt tatccatatc aacgaagacg atagcagcct gccgggtaag
     6361 agtatcaaga acgcccacag tgaccgctgt gtcccgttgg tagatggtgc ttatggtttt
     6421 gtattggctg actttatgtc actcgtggaa gaccgtagaa agactgaggg tgataatgct
     6481 atggtgttca atggcctcaa gctaatgaag aacggctacg gtgagcaggt tagcaagtgg
     6541 tttaaccgaa cgttgctgcc caaagtcctc gctgaccgta gcggcttagc ttttcactcg
     6601 tttagacata cagttgcgac ccagttaaaa caacatgggg tcgagttagc gtatgctcag
     6661 gcgattatgg gtcatagctc agggtctata acttacgacc gttacgcgaa agaggtcgag
     6721 gtggaaacgc tgaaagaaaa attagcggag tcattgtcag taaaaaaaat agacggaaag
     6781 taatcgcata gggatagcgg aatgtgagga cgtaatggtc aaagaatttt tagttagcaa
     6841 agaaaataag ccttcatatg aatgtatgaa tactgctgaa acttgtttgg ggctggctgg
     6901 aatggcttcg gaagattggc aacgtgagca tcattgccgc acaggtataa tatttactgc
     6961 ttttgctatt gaggcaatgt tcatcttcta tagaaaacag gttgaccccg gctacgataa
     7021 gacgcagaaa gagtgcagaa agacaatgca caagaatacg ttaaaattgt gcggtataaa
     7081 caattatatg gggacaaaac cttaccaaat tatcaaagag tgtcttgagg taagagacgc
     7141 tattgctcac ggcgattcgt atacttcgag ctttaatttc tcggcggatc atttggataa
     7201 ccaagacgat attgcaaaag tgtcctggcg gtaaactctg aacaatttcg gggattaact
     7261 gttgagcgcc ttgcaaaata cattgaaatg gcaaagcata ttgaccatga gatttctgat
     7321 aatggttaca ggccttctga aggttacctt ccagcagccg agcgcagaag tctgatgtta
     7381 gcttttggcg tcagtggtgt gtcaatgtgg cccagtgctt gagcgataat acacctcact
     7441 atagaggaat ggacccttaa ctatcactat aggggagaga ccctaagtcc ccaccttaat
     7501 agcagtcacg acccatacac acattcatta agtctcaggc ttccggtagc ggaccgattg
     7561 gaaaccatcc gcagacgcct agtgttaaat caccttggcc tgagaccatt ccttttaacg
     7621 taagctatct tcccttttga caggcgggga gcagtagacc acatccgact ttaagttggc
     7681 tctgtgataa tcgctgcgcg gataactgaa gaaccaagac accattactg aggtctgcca
     7741 tccatttgtg gacaggcgga aactcaaaga ggttagatca gtggtgtctg aggttggagc
     7801 tttgagtctt gaccagcagg ctggaggaga ccatgagttt atcttgttat ggattgtgtt
     7861 atcacaattc caataagaag aactttaagt ccagcctcca ctcccgccaa cccgaagtaa
     7921 aattatggtc gctatgatgt ggtctccatg cttcgggtct tactggaagt actgaacact
     7981 ttcgcgtcaa gttctccgaa gttcattcct gtttgactaa actatcacta tagggaccat
     8041 atctcgcctc tgacctcctg agagacggca agcgagaaac cgtggtccac gctagcaagc
     8101 tagccaccag cctaaggcaa gacagcctct ggtcagcctg tgtgttcttt ggtctcgtga
     8161 cttaagtcta tcaccacaca cgctcggaca tgctgtcgaa ccttcatctc tatggtcatc
     8221 gcagaccact aaccccaatt acctcaagtc attctgtttg cgtcctgttg cctgctggtc
     8281 tccctttacg tgggggcttg agtaggtcag tggggtgcga cttcagggtg caaacaaaag
     8341 gagtacacgt aaatgtccca acaagaaatc agcatcatta acctcgacca gcttgtgtcc
     8401 atgacctcag ttgaaattgc tgagctgaca ggaaaagaac ataaacacgt tttacgagat
     8461 attcgtaata tggtcgaaga gcttaacggg gccaaaactg agcactgctc aactttgagc
     8521 agcgagctta acgggtccaa atttgggctc gttggcgagg aagtatacaa ggacgcgaaa
     8581 ggtgagagcc gaacaatgta tcgtctcgac cgtaagcata ctttcattct ggtcgctggc
     8641 tattccgttc acctccgcgc caagtgttac gaccatattc agacgttaga gcgtcgagtc
     8701 cttcaactgg aagaccagaa gaagcgagca gccattcagt ccgctaaccg ccgaggcgtg
     8761 acttggggcg actactgcaa aacctatggt ctgcccgctc agaagctaat gaccgcactt
     8821 ctccagcatc gtggcctctt tcgtaagaac ccaatcagta acgagtggtc tgtaaatccg
     8881 aagtattccg actgctttcg tatcattaag ccgtcagacc agaagttctc agcaggtggg
     8941 tataacttcc ggttcaacgc taaggggctg gaggtcttcg gtaagcctga gatggttgac
     9001 aagatgcgag gcatccttat tgcattcact ggcaccgacc agcagaagca agagcacctc
     9061 ctgaagctgg cacaatccgg taaggtggag ggaatctaag atgatgattg acccgcgcac
     9121 acctgagggg cgcatgactc tccgttaccg tggctaccgg actgaagtcc tcctcagaga
     9181 gcttggtcta gacccggaag atgagacccg ccagcaccaa tcccgagacg agctaatagc
     9241 ccaactggtc gctatgaaac tgccgcttaa ccgttagtag ctcacctact caatacctaa
     9301 tttaaacacc ttttgagatg accgatgcgg ggagacccgt aggccttact gtgcctgcga
     9361 taccgatagt ctcaactctc aagaggagaa ttacctatga tgttcaacaa caacaactgg
     9421 aagctgtcag tcaccgacat caacctttat gaaaacactg tgagtctgga cggtcagtcc
     9481 tatccgttgt cgctc
//
LOCUS       DAJTSF010000133         9493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000133 DAJTSF010000000
VERSION     DAJTSF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.133"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..190)
                     /locus_tag="OO335_003556"
     CDS             complement(<1..190)
                     /locus_tag="OO335_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR6003793.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="OO335_003557"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="OO335_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6003794.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="OO335_003558"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="OO335_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6003795.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="OO335_003559"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="OO335_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6003796.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="OO335_003560"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="OO335_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="OO335_003561"
     CDS             complement(3262..3381)
                     /locus_tag="OO335_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6003797.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /locus_tag="OO335_003562"
     CDS             complement(3342..3527)
                     /locus_tag="OO335_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003798.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /locus_tag="OO335_003563"
     CDS             3628..3801
                     /locus_tag="OO335_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003799.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="OO335_003564"
     CDS             3803..4147
                     /locus_tag="OO335_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6003800.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="OO335_003565"
     CDS             4151..4486
                     /locus_tag="OO335_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6003801.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="OO335_003566"
     CDS             complement(4543..6864)
                     /locus_tag="OO335_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6003802.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="OO335_003567"
     CDS             complement(6981..7190)
                     /locus_tag="OO335_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003803.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7941..9464)
                     /locus_tag="OO335_003568"
     CDS             complement(7941..9464)
                     /locus_tag="OO335_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6003804.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2659 a         2206 c         2079 g         2549 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatagcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttagtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatc
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc acaaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgctgg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcagccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa tac
//
LOCUS       DAJTSF010000134         9440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000134 DAJTSF010000000
VERSION     DAJTSF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.134"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            39..1154
                     /locus_tag="OO335_003569"
     CDS             39..1154
                     /locus_tag="OO335_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003805.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1833..2051
                     /locus_tag="OO335_003570"
     CDS             1833..2051
                     /locus_tag="OO335_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR6003806.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2269..2511
                     /locus_tag="OO335_003571"
     CDS             2269..2511
                     /locus_tag="OO335_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003807.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="OO335_003572"
     CDS             complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="OO335_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6003808.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="OO335_003573"
     CDS             complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="OO335_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6003809.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="OO335_003574"
     CDS             complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="OO335_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6003810.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="OO335_003575"
     CDS             complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="OO335_003575"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7777..7779),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6003811.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8558..9391
                     /gene="fdhD"
                     /locus_tag="OO335_003576"
     CDS             8558..9391
                     /gene="fdhD"
                     /locus_tag="OO335_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6003812.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2311 a         2315 c         2446 g         2368 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg
      241 tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag
      301 gaaatgtgcc aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg
      361 caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa
      421 tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg
      481 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga
      541 caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      601 tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt
      661 cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag
      721 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga
      781 cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      841 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg
      901 aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta
      961 gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag
     1021 ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt
     1081 tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag
     1141 ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca
     1201 aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc
     1261 atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag
     1321 gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa
     1381 actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg
     1441 aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga
     1501 ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg
     1561 tgctgttagg atgctgacgt ttttcggcta caccattcag attatgtaaa taaatatccc
     1621 tgaatataat atattcatag cacccataac tcatcctggg cgataactaa accatttttg
     1681 agtaataaat gaaacaactg caacgctctt attatttctg gaaaacgcct cgcaaaaaca
     1741 aaatgacatg caaaaatccg cattttttta aatgcttttc cggcgtaatt ttcgcacata
     1801 cattaatgag taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt
     1861 tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat
     1921 ctgcaatttt acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta
     1981 aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc
     2041 ataaggaatg aaaaaaattt ttcattcttg aatataaaaa acagatgccc ttattctggt
     2101 attaatacaa ggctgtttta cttgaactta taataactgc aactgttaca tcgtatctgg
     2161 aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa gattcggcaa
     2221 gggtactttt ctctccatag attcaaaaat gatattggcg agatatttat gaactctctg
     2281 gcaggtatag atatgggcag aattttactc gatttatcga atgaggtgat taagcaactt
     2341 gatgatctcg aggtgcagcg taatcttcct cgtgcggacc tattaaggga agcggtagat
     2401 caatacctga taaatcaatc gcaaacagca agaaccagtg ttcctggcat ctggcaaggg
     2461 tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg aggaatggta atgggaaaaa
     2521 atgatgtcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg
     2581 ccttatccgg cctacaaagt catgcaaatt caatatattg aagtttattg gtaggcctga
     2641 taagcgcagc gcatcaggca atcatacgct tgtcatcagt gacataccca taaccttatc
     2701 gatgtgttgg agggaagaac gaggcaaaca gacaattgcg gcatgatctt gaatgggtaa
     2761 cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg cgacctgcgc
     2821 cacccggaaa acgtcagaga ttactctcct tcacccggaa acaggaacgg gttaatggaa
     2881 ctacgggcgt agccttcttg ctccattcgc gcgtccagta ccagagaggc gaggtcatct
     2941 gccacggctt caactttcgg ttctttttcc tgataaagaa tcttcaggta agtgccgcag
     3001 tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt
     3061 ttgccgctct gctcgcagtt gctgcatttt acgcgcacta cgtgccattc ggtttcacac
     3121 aggttgcagt gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc
     3181 ggcatagagc cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg
     3241 ccggggatca gattggccat ctgcgcccag tagagcgaca gcgcagccca gataaacggc
     3301 gctttatcgc tgctgacgga cgagaaatca gaggcaaaca gcgcgctggc catatcttcc
     3361 agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt
     3421 tcaggtttca gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc
     3481 agaacgtgaa tatccagcgg gggcttgccc tgtgcgctgg cttctttaat gcgcgcggtc
     3541 agatccatct ccagcggatg gtcgtacagc accactttct gggcgtgggc gataagcgca
     3601 gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg
     3661 cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta
     3721 cgtttctcgc tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct
     3781 tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc
     3841 tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga
     3901 tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga
     3961 atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccgc
     4021 tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt
     4081 tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc
     4141 aaaagatatc atcccgattg attaggtttt gatgccagta acggaaaaac atgatgatga
     4201 acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc
     4261 ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg
     4321 ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca
     4381 cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc
     4441 ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg ccgcaaaggt
     4501 agccgcaaag ccaacagccg cgagcggttt ccagatgcct ttccagaatt tcacggtttc
     4561 gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag
     4621 cacgtacatg acgtgtgtac caccgacgcc tgccggatcg tacagacccg cattgtcgta
     4681 accgcgggtt ttcagctcag caacgcgctc gctcgccagc gttttcatcg actctttcgt
     4741 gccgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt gcccaaccac
     4801 cacgcggtca acgcacagcg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg
     4861 aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct cggactggaa
     4921 gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg ctttcaggca
     4981 gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt
     5041 ctgctccact tccgagaagc gcattaccgt ccacgatttg gcgcttaaat cattggggtt
     5101 gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc
     5161 cacctgacag gctttacagc cgatacaggt ggtaacgtcg atgagtttcg ccacttcttc
     5221 ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat
     5281 atcttgagat tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa
     5341 ggacttaaac tccggcgtct gcgtgttcgc atcaccaacg aatggcgtta aagtattggc
     5401 aataaagcct tttttagcta cgccttcata gccccagtga atcggaatac cgatggtatc
     5461 gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg ctttggcttt
     5521 gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc caagtttatt
     5581 cgccagcgac tccccgattt ccacaaactg ctctggctgc aaaatcgcgt tcaacagcgc
     5641 gtgtttggtc cagtagtgga agtgctcggt cagacgatag gtggttccga catacgggaa
     5701 cttatcggct ttacccaatg cttcggcgtc gtctttaaag atacgtgcag ctgggttcga
     5761 gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc
     5821 cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc cttcctgctg
     5881 catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg gaatatccca
     5941 gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc acgggttacc
     6001 ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc
     6061 ccagcccagc gtgttaccga ggccagacgg atcggcgtta tcacgacgcg ccatctggtt
     6121 gccttccggc gtccagctac cggcgaaaat ccagcagcca caggaggttg taccgtcatc
     6181 gcgcaattgg gcgaacgagc taagttgttg gcctttcttg acgataaccg caccggttgc
     6241 cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt cttccgactt
     6301 cggctcatgc ggaatggcgt agttccaggt catgttcagc acctgatccg ggttcgcgcc
     6361 accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga tctcaccgtc
     6421 agtcagcgca atccccgggg cgtccgcacc tttccagtgc cactgcaacc agcgacctga
     6481 gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga acacttcggt
     6541 ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt
     6601 ttcagtgttc agtgggtcga tggtgacgag gaacttcagt ttcgacagac aaccgatcac
     6661 tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac
     6721 cttgccctct ttcatcatct cgaagtattg caggacgtcg tagcctttat cccacttcgg
     6781 caaccagtca aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat
     6841 catagagacg aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg
     6901 ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc ttggcagcgt
     6961 catgtaacct ggcagactct gcgacagtag ccccaggtcc gtcagcccct gaatattgga
     7021 gtgaccgcgc agggcgttaa cgccgccgcc ggccatcccc atattgccga gcagcagctg
     7081 gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc agccgagggc
     7141 atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt cgcagacttt
     7201 caggaacgcg tcttttggcg taccacagat gttttcaacc acatctggcg tgtagcggga
     7261 aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg tatcgcgttt
     7321 ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact tgcgtttttc
     7381 cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct
     7441 ggcgttggta taggcttcgg tgtattcgtg gttgaatttt tcattgttca gcaggtacag
     7501 caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc
     7561 taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc cgttgtgaat
     7621 tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat
     7681 caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc
     7741 aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac
     7801 cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga
     7861 cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc cttcggcgtt
     7921 ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc
     7981 ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt
     8041 cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc
     8101 gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt tggcgttttt
     8161 tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt
     8221 attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct
     8281 gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc agatcttaaa
     8341 gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt
     8401 tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aaggggccgc
     8461 aaaggtccag aattggctca atttccctcc atctaggaag ggtttgtaac agaataccat
     8521 aatgttggtg tgtatgttct tatctggtta agagaaagtg aaaaaaacac agcgaaaaga
     8581 aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga
     8641 tttacaacac ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc tggtctacaa
     8701 cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg
     8761 tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca tggatgtcgt
     8821 tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct ttatggggtt
     8881 gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg gcgtggagca
     8941 acttaatgac atcggaaaac cggtgcagcc gctaccgttc acccagacgt ttgatctcaa
     9001 caaactggat gatgcattac gtcatctcaa cgattttcag ccagtggggc gactgactgg
     9061 ttgtactcac gccgctgcct ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga
     9121 cgtgggtcgc catgtggcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag
     9181 ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg tgcaaaagtc
     9241 ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacgc ttgctgtaga
     9301 agtggctgag cgctgtaatc tgacactggt aggtttttgt aaaccgggtc gggcaacggt
     9361 ttatacccat ccgcagcgtt tgagcaatta attaaaataa gtcctgcgat atatattaaa
     9421 ttgcaggact tattcatttc
//
LOCUS       DAJTSF010000135         9291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000135 DAJTSF010000000
VERSION     DAJTSF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.135"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="OO335_003577"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="OO335_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6003813.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="OO335_003578"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="OO335_003578"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6003814.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="OO335_003579"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="OO335_003579"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6003815.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="OO335_003580"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="OO335_003580"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6003816.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="OO335_003581"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="OO335_003581"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6003817.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="OO335_003582"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="OO335_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6003818.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..6799)
                     /gene="nth"
                     /locus_tag="OO335_003583"
     CDS             complement(6164..6799)
                     /gene="nth"
                     /locus_tag="OO335_003583"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6003819.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="OO335_003584"
     CDS             complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="OO335_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6003820.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="OO335_003585"
     CDS             complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="OO335_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6003821.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="OO335_003586"
     CDS             complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="OO335_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6003822.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2384 a         2326 c         2357 g         2224 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg
     5821 cggcgatatg ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac
     6361 gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt
     6421 ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat
     6481 cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt
     6541 ctgctttgct gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta
     6601 gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa
     6661 cactgatatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac
     6721 tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca
     6781 gacgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc
     6841 agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat
     6901 cagtcccaag ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt
     6961 gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc
     7021 aaacaacgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt
     7081 tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc
     7141 gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc
     7201 taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac
     7261 cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag
     7321 cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag
     7381 agcgttagtg gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag
     7441 tgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta
     7501 ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc
     7561 gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa
     7621 atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc
     7681 cgcaaaatgg gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt
     7741 ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag
     7801 cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg
     7861 tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt
     7921 taccagatag caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc
     7981 aaataacgtc ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt
     8041 gatggccgca gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt
     8101 tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta
     8161 gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg
     8221 atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag
     8281 ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag
     8341 catagtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa
     8401 ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg
     8461 aatcgctttc tgccatagca accacacgcc accagccagc caggcgagat ttacccattg
     8521 ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga
     8581 atgacctgcg cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc
     8641 aatacctaag cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac
     8701 ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct
     8761 ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa
     8821 cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc
     8881 cagtacgacc atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt
     8941 cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac
     9001 gagagcttcg gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca
     9061 accaaaaaac cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat
     9121 aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat
     9181 ttagtcctcg tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc
     9241 cgcggcaact gcggctttgc gcggatcgac ctgttcttct ggttccgcat t
//
LOCUS       DAJTSF010000136         9242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000136 DAJTSF010000000
VERSION     DAJTSF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.136"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO335_003587"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO335_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6003823.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO335_003588"
     CDS             1828..3147
                     /locus_tag="OO335_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6003824.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO335_003589"
     CDS             3445..3486
                     /locus_tag="OO335_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003825.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO335_003590"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO335_003590"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR6003826.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO335_003591"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO335_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO335_003592"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO335_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO335_003593"
     CDS             5815..6711
                     /locus_tag="OO335_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6003827.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OO335_003594"
     CDS             6708..7682
                     /locus_tag="OO335_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6003828.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO335_003595"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO335_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO335_003596"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO335_003596"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6003829.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2259 a         2257 c         2317 g         2409 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca
     9241 ag
//
LOCUS       DAJTSF010000137         9191 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000137 DAJTSF010000000
VERSION     DAJTSF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.137"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..904)
                     /locus_tag="OO335_003597"
     CDS             complement(<1..904)
                     /locus_tag="OO335_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR6003830.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNV"
     gene            964..1242
                     /locus_tag="OO335_003598"
     CDS             964..1242
                     /locus_tag="OO335_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003831.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="OO335_003599"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="OO335_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6003832.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="OO335_003600"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="OO335_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6003833.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="OO335_003601"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="OO335_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6003834.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="OO335_003602"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="OO335_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6003835.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..4880
                     /locus_tag="OO335_003603"
                     /pseudo
     CDS             4234..4880
                     /locus_tag="OO335_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4922..5452)
                     /locus_tag="OO335_003604"
     CDS             complement(4922..5452)
                     /locus_tag="OO335_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6003836.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5608..6174)
                     /gene="slp"
                     /locus_tag="OO335_003605"
     CDS             complement(5608..6174)
                     /gene="slp"
                     /locus_tag="OO335_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6003837.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6421..7644)
                     /locus_tag="OO335_003606"
     CDS             complement(6421..7644)
                     /locus_tag="OO335_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003838.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8225..>9191)
                     /locus_tag="OO335_003607"
     CDS             complement(8225..>9191)
                     /locus_tag="OO335_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6003839.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2681 a         1827 c         1922 g         2761 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgttggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggttac gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg
     4381 aaatatggtt ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt
     4441 gctgctcagg tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac
     4501 caaaatattg taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg
     4561 ttattggcag cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc
     4621 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
     4681 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
     4741 ctgatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
     4801 ttcatgccac aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga
     4861 aaggcgtttc aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa
     4921 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
     4981 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct
     5041 ccagaatatc ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
     5101 gcgaaagtga cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
     5161 aatcaatgat agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt
     5221 taatattaaa gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga
     5281 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat
     5341 gcaacattac gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa
     5401 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
     5461 cctggtgcgg acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt
     5521 ataataataa tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg
     5581 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
     5641 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
     5701 atcccacagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
     5761 catattcact tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg
     5821 aatggtaccg aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct
     5881 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc
     5941 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
     6001 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
     6061 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
     6121 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
     6181 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
     6241 tgaggtgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag
     6301 ttcaattccg ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt
     6361 gttttaaaat tacataaaat ctatttttat tcaatgatta taaagtgcta ataatcaata
     6421 ttatggtatt atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc
     6481 tatttccacc ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg
     6541 gtatgaaatc ttttcgggtt gaagtgtctc gtaatgaaat tctatgaatt catttttaga
     6601 cattgttgcc catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg
     6661 tttataacat atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt
     6721 cactaccttt gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa
     6781 agagttgtca ttaatagtta acaatgtaga aaacaaacca ttgatttgtt tgtcaagatg
     6841 ttgttcatat ttatgtagtt tggggatggg attttccttg tgtgaaaaaa tagtgttaaa
     6901 taaatttgcg tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt
     6961 taggactagc ttggacatat cttgccctgt catgttgaaa tcaataaagt cgcatccagc
     7021 aagggcttcc atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa
     7081 gtacaagctt tgaatgtctc ttatatcagt tggtaagtca gctcgttgcc cagcaaaatt
     7141 tttattatcg gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag
     7201 aattttagtt atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac
     7261 agaatcctta tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg
     7321 ttgcgtaagt ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac
     7381 cacaactttt gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg
     7441 ttggagttgt tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatgt
     7501 ggaattaatg ggttgttgtt cttcccaatt atcacaaggc cacttcggtg tgtctggcgt
     7561 tttaacatta tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat
     7621 acctggggta aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt
     7681 ttttaaattg gataattaga acaggaatgg attttcttct acacacaata aatataatgc
     7741 gttatatctt cctgatataa ttacctttaa attccctttc atttccaccc ggtaatattt
     7801 gtgtataaaa aaagggcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaaa
     7861 cgctcatttt aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg
     7921 ttttagatgt gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga
     7981 ctgaaatctg ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga
     8041 taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg ttgaccgagc
     8101 cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt atcctgtcta
     8161 tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta ctgctggcat
     8221 ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct aatataaccg
     8281 tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc acttccgtca
     8341 ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc attccttttg
     8401 tcatcagaac agatgccaga gggataaccg cctcagcccg gatatacagc ggaataccga
     8461 caacggcgct taatgggatg gcgaagggat tatctgctcc tgcatgagcg gcaatccacg
     8521 ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa agatatggca
     8581 gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga ttaatactta
     8641 ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta gccgctggac
     8701 tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt gctggagcac
     8761 aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc agagaatcca
     8821 ggataatact ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac aaggtgactt
     8881 tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat ggtgaaacaa
     8941 ataaaaaagt gagagtcgga ccaaacccgg ctttcgctga caacaatcca cgtaacatgg
     9001 gtattgtcga gcaactacag aacggggtaa cggctcccaa cagagcagcc aggagatagc
     9061 ctctcccttt tctggccccc atcatctgct ggattttatg gtctggcacc ttttgtctta
     9121 tcaggctgac accggcacta atcactataa acagcaacga aagctcgacg gcgagaaaca
     9181 caaacatctc t
//
LOCUS       DAJTSF010000138         9187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000138 DAJTSF010000000
VERSION     DAJTSF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.138"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..924
                     /locus_tag="OO335_003608"
     CDS             <1..924
                     /locus_tag="OO335_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6003840.1"
                     /translation="LGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAP
                     PALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRP
                     QLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAI
                     PFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAV
                     AFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDT
                     THSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1176..2093
                     /gene="oxyR"
                     /locus_tag="OO335_003609"
     CDS             1176..2093
                     /gene="oxyR"
                     /locus_tag="OO335_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6003841.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="OO335_003610"
     CDS             complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="OO335_003610"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6003842.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3797..4447
                     /gene="fabR"
                     /locus_tag="OO335_003611"
     CDS             3797..4447
                     /gene="fabR"
                     /locus_tag="OO335_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6003843.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4447..4806
                     /locus_tag="OO335_003612"
     CDS             4447..4806
                     /locus_tag="OO335_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6003844.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="OO335_003613"
     CDS             complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="OO335_003613"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6003845.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6060..6250
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6315..8159
                     /gene="btuB"
                     /locus_tag="OO335_003614"
     CDS             6315..8159
                     /gene="btuB"
                     /locus_tag="OO335_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR6003846.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8104..8961
                     /gene="murI"
                     /locus_tag="OO335_003615"
     CDS             8104..8961
                     /gene="murI"
                     /locus_tag="OO335_003615"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6003847.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2102 a         2222 c         2483 g         2380 t
ORIGIN      
        1 ctgggtattg gcgaagcgcc atttatgcct gcgggagtaa agtcgatcac cgactggtat
       61 gcacaaaaag aacgcggcac ggcgctgggg atttttaact catctaccgt tatcggtcag
      121 gctatcgcgc ctcctgctct tgtattgatg cagctggcat ggggctggcg gacgatgttc
      181 gttatcatcg gcgtggcagg gattctggtt gggatctgtt ggtacgcgtg gtatcgcaac
      241 cgggcgcagt ttgtcctgac tgacgaagaa cggacgtatc tctccgcgtc ggttaaaccg
      301 cgtccacagc tgcaatttag cgagtggctg gcgctgttta agcatcggac aacctggggg
      361 atgattttag gtttctctgg cgtcaattat accggttggc tttacattgc gtggttaccc
      421 ggttatttgc aagccgaaca aggtttcagt ctggcgaaaa ccggttgggt agcggcgatt
      481 cctttcctcg cggcggcggt cgggatgtgg gttaacggta ttgtggtcga tcggctggcg
      541 aaaaaaggct acgacctggc gaagacgcgt aaaacggcta ttgtctgcgg tttgatgatg
      601 tcggcattag gcacgttgct ggtcgtgcaa tcttcctcgc cagctcaggc ggtagcgttt
      661 atctcaatgg cgctgttctg cgtgcatttc gcgggaacgt ctgcatgggg actggtgcag
      721 gtgatggtgt cagaaacaaa agtggcttcc atcgccggga ttcaaaactt tggcagtttt
      781 gtttttgctt cctttgctcc gatcgtaacc ggttgggtag tggataccac acactcgttt
      841 aatctggcgc tggttatcgc ggcctgtgtg acgttcaccg gggcgctgtg ttacttcttt
      901 attgtcaaag atcgcattga gtaatcgtta ctatcaggct ctcttgctgt gggcctgtag
      961 aataaaaaaa agcggatcct ggagatccgc aaaagttcac gttggcttta gttattcgag
     1021 ttgagaaact ctcgaaacgg gcagtgactt caagggttaa aagaggtgcc gctccgtttc
     1081 tgtgagcaat tatcagtcag aatgcttgat agggataatc gttcattgct attctaccta
     1141 tcgccatgaa ctatcgtggc gatggaggat ggataatgaa tattcgtgat cttgagtacc
     1201 tggtggcatt ggctgaacac cgccattttc ggcgtgcggc agattcctgc cacgttagcc
     1261 agccgacgct tagcggccaa attcgtaagc tggaagatga gctgggcgtg atgttgctgg
     1321 agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat gctgctggtg gatcaggcgc
     1381 gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc aagccagcag ggtgagacga
     1441 tgtccggacc gctgcatatt ggtttgattc ctacggttgg accatacctg ctaccgcata
     1501 ttatcccgat gctgcaccag acatttccaa agctggaaat gtatctgcat gaagcacaga
     1561 cccaccagtt actggcgcaa ctggacagcg gcaaactcga ttgcgtgatc ctcgcgctgg
     1621 tgaaagagag cgaagcattc attgaagtgc cgttgtttga tgagccaatg ttgctggcta
     1681 tctatgaaga tcacccgtgg gcgaaccgcg aatgcgtact gatggccgat ctggcagggg
     1741 aaaaactgct gatgctggaa gatggtcact gtttgcgcga tcaggcaatg ggtttctgct
     1801 ttgaagccgg ggcggatgaa gatacacact tccgcgcgac cagcctggaa actctgcgca
     1861 acatggtggc ggcaggtagc gggatcactt tattgccagc gctggctgtg ccgccggagc
     1921 gcaaacgcga tggggttgtt tatctgccat gcattaagcc ggaaccatgc cgcactattg
     1981 gcctggttta tcgtcctggc tcaccgctgc gcagccgcta tgagcagctg gcagaggcca
     2041 tccgcgcaag aatggatggc catttcgata aagtgttaaa acaggcggtt taaaccgttt
     2101 aacgcagcta cccgataggc ttccgccatc gtcgggtagt taaaggtggt gttgacgaag
     2161 tactcaatag tgttgccgcc acctttctgt tccataatcg cctgaccgat atgaataatt
     2221 tcggcagcgc gctcgccaaa gcagtgaata cccagaatct cttttgtttc ccgatggaac
     2281 aaaattttca gcgtgcccac gttcatgccg acgatttgtg cacgtgccag atgtttgaac
     2341 tgggcgcggc ccacttcata cggcactttc atcgcggtca gctgctgttc ggttttgccc
     2401 acagagctga tttccgggat ggtgtaaatg ccggtaggga tatcttcaat cagatgtgcg
     2461 gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc cctggtcata ggccgctgat
     2521 gccaggctcg gataaccaat cacgtcgccc accgcgtaaa cgtgcggctg tgcggtctga
     2581 tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt ccagcccaat gttctgtaac
     2641 gccagcgaat cggtattacc ggtacgaccg ttggcataga gcaggcagtc agctttcagt
     2701 tttttacccg acttcagatg catgatcaca ccgtcgtcac agctttcgat cttctcgtac
     2761 tcttcgttgt gacgaatcac tacgccactg ttccagaagt ggtaggagag agaatctgac
     2821 atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt tgatcagatc cacttttaca
     2881 tccataccgc ggaagatcga cgcatattca cagccgatca ctccagcacc atagataagt
     2941 acatggcgcg gttcgtggtg catgctaaga attgagtcgc tgtcgtaaat gcgtggatgg
     3001 gtgaaatcaa catctgttgg atgatatgga cgagagccgc aggcaataac aaatttttca
     3061 gcggttagtg tttcaacgct gccgtccggg caatccagcg ccaacgtatg ctcgtcaaca
     3121 aagcgagcgt tcccctgcaa tatttcacag tgattacgtt cgtaaaatcc ctgacgcatg
     3181 cgcgtttgtt gattaatcac gttatcggca tggttaagga tatcggcaaa agaagagcgg
     3241 agcagtcggg aatggtcgct gtatagtggg ttttgattga attctataat gcggctgacg
     3301 gcgtgacgga gagctttcga cgggatggtg ccccagtggg tgcaaccgcc gccaacattt
     3361 tgataacgct cgataactgc gacgcgcgca ccttgcttaa ccaggcccat tgcagcgcct
     3421 tcgccgccgg ggccggaacc tattactatg gcatcgtaat cgtaggaatg tggcatggta
     3481 gggcttacct gttcttatac ataaaagcaa cagaatggta acattctatc gcgggtaagc
     3541 caattgatcc ccgtcattta tctggctata tcctgagcgg cctttgcttt gtctgtttct
     3601 tacttttgcc ctgacgtttt attggatttt tatcgacgat actctccgtt taagcggcag
     3661 gtttccgctg tacgtaaaag aaccggccag taaatatgtt ttattgcgtt accgttcatt
     3721 cacaatactg gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg
     3781 caaggattca gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc
     3841 gctggtggaa gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt
     3901 gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga
     3961 cgtagacgaa ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat
     4021 gcgccaggcg cgtcagcgta tcgccaaagg cgggagtgtg atccgtacct cggtctccac
     4081 atttatggag ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc
     4141 cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga
     4201 acttgcggac tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc
     4261 cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag gcgttggacg tcggcgtcga
     4321 acaacgtcgg caattagaag agcgactggt actgcaactg cgaatgattt cgaaaggggc
     4381 ttattactgg tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga
     4441 cgagtaatga aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggt
     4501 ttatcgatta atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc
     4561 ccgattattt ccctggtgct gacggtttac tgcctgcatc aacgttatct taatcgcacc
     4621 atgtcggtag gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac
     4681 agcacggtag ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc
     4741 tccgtcatta tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct
     4801 gagtaatcgg tatcatgccg gatgctggcg catccggcat gggttttact tcgcggtcag
     4861 taatacgccg cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc
     4921 gaccttgtgc gtctggctta atgtttccag atttttgcat aacgtttccg gattgcagga
     4981 gatatacaga atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct
     5041 gcgcggtggg tcgacaaaaa tggtttcgaa ctgatagctc tttaagtcga tcccttgcag
     5101 gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat tcttctgccg ccatacgaat
     5161 aatttgtacg ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgactgacgg
     5221 tttggcgatt tcggtggcta atacccgatc aaaattgcgc gccagcgcta atgaaaagtt
     5281 accgttgccg cagtacagct ccagtaaatc gccttttgag cctttggtta cgtccagcgc
     5341 ccattccagc atctgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg
     5401 acgatagatc atctctttcc ctgcgaccgg cagacgctcg tcgatgtaat cttgatccag
     5461 cgcgattttg gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgcgcgcag
     5521 tgcatcgcgc agtgcttccg cttcctgacg ccactcatca tccagctttt tatggtacag
     5581 cagggaaacc accgcctgat tactcagcgt ggtgaggtaa tcaatctgga acaacttgtg
     5641 gcgcagaacg ggattattac gcacacccgc aatcatcgcc gtcatcaact ggttgatgag
     5701 ttcactggcg gcggggaagc tatccacgcg gatgcggctt ttggtttgtt gatcgaaaat
     5761 gatgtgatac aggtcatcgc catcgtgcca gatgcggaac tccgcgcgca tccggtaatg
     5821 actgaccggc gagcgaaaca cttccggaac caggtcagaa aacggtgcca tcatactttg
     5881 caaacgtacc actttttcgg ctaactgcgc ttcatactgt tctgttggaa ggtgttcggg
     5941 ggtcatgatg tatcctgaaa aattaaagta cgcggcgatt gtagggattg ctcatcagat
     6001 gtccagatct tgatgaattc ctatttgtga gctacgtctg gacagtaact tgttacaacc
     6061 tgtagcatcc acttgccggt cctgtgagtt aatagggaat ccagtgcgaa tctagagctg
     6121 acgcgcagcg gtaaggaaag gtgcgatgat tgcgttatgc ggacactgcc atccggtggg
     6181 aagtcatcat ctcttagtat cttagatacc cctccaagcc cgaagacctg ccggccaacg
     6241 tcgcatctgg ttctcatcat cgcgtaatat tgatgaaacc tgcggcatcc ttcttctatt
     6301 gtggatgctt tacaatgatt aaaaaagctt cgctgctgac ggcgtgttcc gtcacggcat
     6361 tttccgcttg ggcacaggat accagcccgg atactctcgt cgttactgct atccgttttg
     6421 aacagccgcg cagcactgtg cttgcaccaa ccaccgttgt gacccgtcag gatatcgacc
     6481 gctggcagtc gacctcggtc aatgatgtgc tgcgccgtct tccgggcgtc gatatcaccc
     6541 aaaacggcgg ttcaggtcag ctctcatcta tttttattcg cggtacaaat gccagtcatg
     6601 tgctggtgtt aattgatggc gtccgcctga atctggcggg ggtgagtggt tctgccgacc
     6661 ttagccagtt ccctattgcg cttgtccagc gtattgaata tatccgtgga ccgcgctccg
     6721 ctgtttatgg ttccgatgca ataggcgggg tggtgaatat catcacgacg cgcgatgaac
     6781 ccggaacgga aatttcagca gggtggggaa gcaatagtta tcagaactat gatgtctcta
     6841 cgcagcaaca actgggggat aaaacacggg taacgctgtt gggcgattat gcccatactc
     6901 atggttatga tgttgttgcc tatggtaata ccggaacgca agcgcagaca gataacgatg
     6961 gttttttaag taaaacgctt tatggggcgc tggagcataa ctttactgat gcctggagcg
     7021 gctttgttcg cggctatggc tatgataacc gtaccaatta tgacgcgtat tattctcccg
     7081 gttcaccgtt gctcgatacc cgtaaactct atagccaaag ttgggacgcc gggctgcgct
     7141 ataacggcga actgattaaa tcacaactca ttaccagcta tagccatagc aaagattaca
     7201 actacgatcc ccattatggt cgttatgatt cgtcggcgac gctcgatgag atgaagcaat
     7261 acaccgtcca gtgggcaaac aatgtcatcg ttggtcacgg tagtattggt gcgggtgtcg
     7321 actggcagaa acagactacg acgccgggta caggttatgt tgaggatgga tatgatcaac
     7381 gtaataccgg catctatctg accgggctgc aacaagtcgg cgattttacc tttgaaggcg
     7441 cagcacgcag cgacgataac tcacagtttg gtcgtcatgg aacctggcaa accagcgccg
     7501 gttgggaatt catcgaaggt tatcgcttca ttgcttccta cgggacatct tataaggcac
     7561 taaatctggg gcaactgtat ggcttctacg gaaatccgaa tctggacccg gagaaaagca
     7621 aacagtggga aggcgcgttt gaaggcttaa ccgctggggt gaactggcgt atttccggat
     7681 atcgtaacga tgtcagtgac ttgatcgatt atgatgatca caccctgaaa tattacaacg
     7741 aagggaaagc gcggattaag ggtgtcgagg cgacagccaa ttttgatacc ggaccactga
     7801 cgcatactgt gagttatgat tatgtcgatg cgcgcaatgc gattaccgac acgccgttgt
     7861 tacgccgtgc taaacagcag gtgaaatacc agctcgactg gcagttgtat gacttcgact
     7921 ggggtattac ttatcagtat ttaggcactc gctatgataa ggattactca tcttatcctt
     7981 atcaaaccgt taaaatgggc ggtgtgagct tgtgggatct tgcggttgcg tatccggtca
     8041 cctctcacct gacagttcgt ggtaaaatag ccaacctgtt cgacaaagat tatgagacag
     8101 tctatggcta ccaaactgca ggacgggaat acaccttgtc tggcagctac accttctgaa
     8161 ccacgtccca ccgtgctggt gtttgactcc ggcgtcggtg ggttgtcggt ctatgacgag
     8221 atccggcatc tcttaccgga tctccattac atttatgctt tcgataacgt cgctttcccg
     8281 tatggcgaaa aaagcgaagc gtttattgtt gagcgagtgg tggcaattgt caccgcggtg
     8341 caagaacgtt atccccttgc gctggctgtg gtcgcttgca acaccgccag taccgtttca
     8401 cttcctgcat tacgcgaaaa gttcgacttc ccggttgttg gtgtcgtgcc ggcgattaaa
     8461 cctgctgcac gtctgacggc aaatggcatt gtcggattac tggcaacccg cggaacagtt
     8521 aaacgttctt atactcatga gctgatcgcg cgtttcgcta atgaatgcca gatagaaatg
     8581 ctgggctcgg cagagatggt tgagttggct gaagcgaagc tgcatggcga agatgtttct
     8641 ctggatgcac taaaacgtat cctacgcccg tggttaagaa tgaaagagcc gccagatacc
     8701 gttgtattgg gttgcaccca tttccctcta ctacaagaag aactgttaca agtgctgcca
     8761 gagggaaccc ggctggtgga ttctggcgca gcgattgctc gccgaacggc ctggttgtta
     8821 gaacatgaag ccccggatgc aaaatctgcc gatgcgaata ttgccttttg tatggcaatg
     8881 acgccagaag ctgaacaatt attgcccgtt ttacagcgtt acggcttcga aacgctcgaa
     8941 aaactggcag ttttaggctg atttggttga atgttgcgcg gtcggaaaat tattttaaat
     9001 ttcctcttgt caggccggaa taactcccta taatgcgcca ccactgacac ggaacaacgg
     9061 caaacacgcc gacgggtcag cggggttctc ctgagaattc cggcagagaa agcaaaaata
     9121 aatgcttgac tctgtagcgg gaaagcgtat tatgtacacc ccgcgcagct gagaaaaagc
     9181 gaagcgg
//
LOCUS       DAJTSF010000139         9029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000139 DAJTSF010000000
VERSION     DAJTSF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.139"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO335_003616"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO335_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6003848.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO335_003617"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO335_003617"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6003849.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO335_003618"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO335_003618"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6003850.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO335_003619"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO335_003619"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6003851.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="OO335_003620"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="OO335_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6003852.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /locus_tag="OO335_003621"
     CDS             5562..6035
                     /locus_tag="OO335_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6003853.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="OO335_003622"
     CDS             6064..6606
                     /locus_tag="OO335_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR6003854.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="OO335_003623"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="OO335_003623"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6003855.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="OO335_003624"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="OO335_003624"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6003856.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="OO335_003625"
     CDS             8003..8260
                     /locus_tag="OO335_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6003857.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="OO335_003626"
     CDS             complement(8236..8790)
                     /locus_tag="OO335_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6003858.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2259 a         2192 c         2252 g         2326 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagag
//
LOCUS       DAJTSF010000140         8757 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000140 DAJTSF010000000
VERSION     DAJTSF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.140"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            5..436
                     /locus_tag="OO335_003627"
     CDS             5..436
                     /locus_tag="OO335_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR6003859.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OO335_003628"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OO335_003628"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6003860.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OO335_003629"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OO335_003629"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6003861.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OO335_003630"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OO335_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6003862.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="OO335_003631"
     CDS             complement(4438..5145)
                     /locus_tag="OO335_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6003863.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OO335_003632"
     CDS             complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OO335_003632"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6003864.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6003..6206)
                     /locus_tag="OO335_003633"
     CDS             complement(6003..6206)
                     /locus_tag="OO335_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6003865.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6395..7873
                     /locus_tag="OO335_003634"
                     /pseudo
     CDS             6395..7873
                     /locus_tag="OO335_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7870..>8757
                     /locus_tag="OO335_003635"
     CDS             7870..>8757
                     /locus_tag="OO335_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6003866.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEP"
BASE COUNT         2031 a         2387 c         2250 g         2089 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tgacgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc cgccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc
     5821 tcaaccacca gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc
     5881 agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca
     5941 aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac
     6001 ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc
     6061 gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc
     6121 gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca
     6181 gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct
     6241 ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga
     6301 aaagtgagga aatgctgcga gtataacgct tactcgtgag gatttatgtt ctgctggcag
     6361 tttcgtgatc aacacctcgc gccaggatgg ctttatgcac tatcgcaatg tggtttgttt
     6421 gttgtcttgt tcattgtttt tatcgtcggc atggggatgt cggctttccg cacctgaaca
     6481 taatatttac cagaaacagg gaaaaggcgt ggtgtacctg cgcccttata aagaaacaaa
     6541 tctttctcta cctgacgtga attacaagag tttgcgtcgg ttacccaatt tattaataga
     6601 cccgacaaca ctagacgaat gggataaaga accgcctctg actgatttga caaccgacta
     6661 cctctatgag ggcgcacagg cctggtatcc tcattattca tggcatagcg atggtcgcaa
     6721 tatcctctac gcgggtgaag ttgtgcaaaa ccctcccggc acacctcccg tcgatgtcgc
     6781 ctcgttcaag gcatggggtg attttgccgc agataaacac agtctctatt ttgaaggcaa
     6841 gcgcaccgat gacaatggtg gtggaaatag cttggatatc aaaacgctgc atcaggtgga
     6901 attccggcct ccctgggatc ctgacttact ggggctgata ttacgtgacg ctaatttctt
     6961 gtatatcaat gggcatcgcc tcgctgaccc cgagagtttc cgtgtgctgg cgcagaagtc
     7021 ctgggatcag cgaggaaagt tttccaccac gtttaacccc tgcattgccg taccatttgg
     7081 cccctgggat accctggctc gtacacggac caaaatcctg ctcaacggcg aacagcttga
     7141 tgccgacccg gacacctttt ccgtcgtgcg ctggatgccc ggctcactct tgacctggcg
     7201 tgataaaaac gggctgcagc gtaaagtcct cgacaaggaa aatctggcgt gggatgaaga
     7261 tttaacaaag cactgtctgg atttttctct gctggaaaag aaagtgttct ggcgtaaagg
     7321 acctgcttgt aaacaggaag aattacccgg actcgatccg gagcagtttc aacccatcag
     7381 tgatgctgtc gcccagcatc aggactcgct ttataccatc atcgaaacag agtctggtaa
     7441 ccgtaagctg gagatcgtga aacttgatga tcccaatctt attatcaaca aacgtttcaa
     7501 cgccgggaaa cgccacggct atttacttac gcgtgccgaa gggtagccat accattccgg
     7561 tttacacgtg tttgaatctg acggaccgct gatcttactg gataaccgtt ctccggatga
     7621 acgcgaagcc catcttaatg accatccctt tttgcgcaga tggtatgccc gcgataaccg
     7681 ctacatttac agctttgatg gcgcgcagct ctggcgatac cgcaccgctg atccgaaaca
     7741 agttcgctta atctggaagg aacaacattc gggatatgtc tatggcgtaa attacaaaac
     7801 gggatatctg gacggaaaaa ttactgatga cggcgaattt attcctgccc cgcgcaatga
     7861 ggcgacaaaa tgaagggtca aataatcaca gttgcactca tcctgttggg acacacattt
     7921 ctgacgcctg ctgtacaggc catagggttc gactattaca atgatcacgg tgttatgtct
     7981 tacggtaaag gttattgggg ggataagaaa atcattgccc agtttccgga gatgagcagg
     8041 gccggtttgc gccttgtgaa aaatatctct gggaaaacgg gttatatacc ttccgatgaa
     8101 ataagcatag aaaatgagga ataccactgg gtgacagacg gccgcgttat tctctggcgt
     8161 ggcaaaatcg ttagcaatcc accgggaaca ctcactgtcg atattgccag ctttcaggct
     8221 atgggccgct tcgcggtcga taaatatagc ctctattttg acggacagcg caccgaaagt
     8281 aatagcggcg cgtcacgtgt tgagctggcg acactaaaag ctatcgaagg taactctacc
     8341 acgctggtgg atagtaaaaa tctctacttg tccggtcgac gtcaggggag cagcagtgat
     8401 gttactgtat tagaaaaaag atggtggggt attaatccac gtcttatgag tgtaaacaga
     8461 aattcatatt ccaatgatct gcttatccgc agtgggcaga atatttattt aaatggcgtt
     8521 caccttacgg cgaatgcaga ctcatttgag ataattcgct ggatacctca ttcactgctg
     8581 gtttttcgcg acaataaggg tctgcatcgt tatccctttg gtcaattatc aggcaaagcg
     8641 atacccgtag atgatgacgt ctcttttgaa gtaggggaaa gtcgcgttcg ctggcgtaaa
     8701 cagctcacgc ccgaccgtca gtggagcaag tggatagacc taccaggtat tgaacct
//
LOCUS       DAJTSF010000141         8669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000141 DAJTSF010000000
VERSION     DAJTSF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.141"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(803..1999)
                     /gene="hemY"
                     /locus_tag="OO335_003636"
     CDS             complement(803..1999)
                     /gene="hemY"
                     /locus_tag="OO335_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6003867.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2002..3207)
                     /gene="hemX"
                     /locus_tag="OO335_003637"
     CDS             complement(2002..3207)
                     /gene="hemX"
                     /locus_tag="OO335_003637"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6003868.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3229..3969)
                     /gene="hemD"
                     /locus_tag="OO335_003638"
     CDS             complement(3229..3969)
                     /gene="hemD"
                     /locus_tag="OO335_003638"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6003869.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3966..4907)
                     /gene="hemC"
                     /locus_tag="OO335_003639"
     CDS             complement(3966..4907)
                     /gene="hemC"
                     /locus_tag="OO335_003639"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6003870.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5294..7840
                     /gene="cyaA"
                     /locus_tag="OO335_003640"
     CDS             5294..7840
                     /gene="cyaA"
                     /locus_tag="OO335_003640"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6003871.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7880..8200)
                     /gene="cyaY"
                     /locus_tag="OO335_003641"
     CDS             complement(7880..8200)
                     /gene="cyaY"
                     /locus_tag="OO335_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6003872.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8248..>8669)
                     /locus_tag="OO335_003642"
     CDS             complement(8248..>8669)
                     /locus_tag="OO335_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6003873.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1971 a         2240 c         2233 g         2225 t
ORIGIN      
        1 ctggttgtct ggagattcag ggggccagtc tagcagttga cggggtatct gctgcgctgg
       61 ccatgagagg aagagcggcg gcgacagcaa caacaagacg tttgggtgaa aacgaaaatt
      121 ccatgcaaaa tgctccggtt tcatgtcatc aaaatgatga cgtaattaag cattgataat
      181 tgagatccct ctccctgaca ggatgattac ataaataata gtgacaaaaa taaattattt
      241 atttatccag aaaatgaatt ggaaaatcag gagagcgttt tcaatcctac ctctggcgca
      301 gttgatatgt aaggcaggtt tattatatcg cgttgattat tgatgctgtt tttagtttta
      361 acggcaatta atatatgtgt tattaattga atgaatttta tcattcataa taagtatgtg
      421 taggatcaag ctcaggttaa atattcactc aggaagttat tactcaggaa gcaaagagga
      481 ttacagaatt atctcataac aagtgttaag ggatgttatt tcccgattct ctgtggcata
      541 ataaacgagt agatgctcat tccatctctt atgttcgcct tagtgcctca taaactccgg
      601 aatgacgcag agccgtttac ggtgcttatc gtccactgac agatgtcgct tatgcctcat
      661 cagacaccat ggacacaacg ttgagtgaag cacccacttg ttgtcataca gacctgtttt
      721 aacgcctgct ccgtaataag agcaggcgtt tttttatgca tcagaaaggt cccggaggtg
      781 ctttcctccg ggtgagaagg aactactgtg gcgggttatt ctgcaacgtt aacatcaaac
      841 cgtcgcgacg catcgctgca gcttcttccg gcttgtgcag tctgtccagc gcgtcggcaa
      901 gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc cgcgcggaag gcgagcgatg
      961 cttcctgcca ttcaccgtgc ttcatcagtg actgacccaa tgtgctccac aacagcgggc
     1021 gatcaccgac gtttttgatt tgctggcgta gcactttttc cagctgttcc gggttattgg
     1081 ttttcagacg cgggatcggc agcagtaggc gatcgtcata ctggcgtttc aggccgtcga
     1141 tgataatttg ctgggcagta tcatgatcgt cacattcaat aagatgttcc gccattgcca
     1201 cctgcaacgc tacctgatga cgcgttttcc ggctttggtt tttccaccag ttacgcaaac
     1261 cttcactacc gttatcggca cgcgcctgat ccatcaggcc aatccatgcc tgttgttcca
     1321 gcattgcacg atgttcttca tcaccaacat gggctttcgc cattgatggg atgatatcca
     1381 gcagagaact ccatgcccct gtgcggatat acgcctgttc cgccagacgt aatacttcag
     1441 gatggcgtgg cgtaacttcc agcagcttat ccacgccgtg gcgtgcagca tggttttcat
     1501 tacgggccag ttgcagacgt acacgggtga tttctaccgg aatggtgtca ttgccggcca
     1561 gctccgctgc gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt tgttgcgcgg
     1621 cttcggcagc cagtagatag ttcaccaccg gttgttcagc gtgatcggca tttttcgcca
     1681 tcagcttttc aacttgctga taatcacctt ccgccagttt cagcagcgcc tgttcggtct
     1741 gcttacgcgc acggcgacgc ttacgtccga caaaccatcc acgggtgtgc gcgccagtgc
     1801 ggaagatccg ccgcagtagc cactcaatgg caaacagcac taccatcgcc agaatcaata
     1861 tgatcgccag gcccgtgacg ctggtttcga tattgtagtt gtcggtctgg atcagcacat
     1921 aaccctgatg accggcaatc atcgggccaa ccactatccc cgcaatcagc aaaacaaaga
     1981 gtaataacac ttttagcatg gttattctcc ttgcggcgcg gctgccggag tatcaccttg
     2041 cggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt ttagcttccg ttgtccccgc
     2101 tgccggttgt gccagcaggt tacgcacgcg agtctgcatc agtttttcca gcatcgcctg
     2161 gctttgcagg gtttccggaa gatccatcga gatattttgc tggcttaact ggtccacctc
     2221 gtcgaggaac gctttggtgg tggcatcatc agtatcgtag taagcacgta cccaggtgga
     2281 gacgttctcc agcgcctggc gataagtctc ttcctgatga cgcggtacag cttgtgctgc
     2341 gaccagcagg cgagagcgaa tattttcgcg cagatagata tcctgatttg gcgctaacag
     2401 cggtacggcg gtgtcatcac gacggcgaat cgtaatgaag ttgtccataa agttctgcca
     2461 gcttttttgc agattgatac gccattcgct gatggaactg gaaagctctt caccgtctga
     2521 atccatcggc gaaccatcgc tgtcattatc ggccagacgc aggttatcta cctgatttga
     2581 aagctgatta agcttaagga tgatgccgtc ataatccacc tgcgatactg cagaaaggct
     2641 ggcgatatca tcggtaattg cccgacgaac ggtaatcaga ctcgggtcat tcatatccgc
     2701 caggctggcg tctgcacttt tcagcaacgc tgcagaggtc gtgacgtcct gatcgctcca
     2761 cagcttccgt ccggcgagtt tcaccagaaa atcggcctga gccagcagcc aggttttagc
     2821 atcgctgccg gaaatggtgg cgatcttttg ttggacttca tccaactgtt ttgccagcgt
     2881 ttcttgctga cgattcgcct gcttaagctg cgcagcttgt tgcttaataa tgccttccag
     2941 ctcggctttt tggctctcct gggctttttg caatgccgtc agttggttag ccagggcatc
     3001 gctggtggcg gtctgattga cggcctgttg tttaccccag ccatacaaac cgacacccgc
     3061 cgccagagca atagcgatag ccaccgcgct gagaatcaat gcagtattgt tcttactctt
     3121 tttttctgtt gcgacaggtt gtgacgtggt gtccacggcc tccctggtct cttcaaccac
     3181 ggcggaggtt ttttcttgtt ccgtcattat ggcttcctgt tatgagagtt attgtaatgc
     3241 ccgtaaaagc gcatcgttgt cagcgttatc ggcgacctta atgtcttgcc agcccagttc
     3301 ccgggcgagt ttcgccaaac gctcactgac gaccaatagt cgacagtgta gtaaccagtg
     3361 ctcacgatac cattgtggga tcagcgacca gagttgctgc aacatttcac cgctggtaac
     3421 aacgaccatc gtcacctcgc gggattgcca gcgcatcgct tcttctgcac catcgtaatg
     3481 gattgcgcat cgttgataac attcacaaaa agtgacctca gcaccgcgca ccgtcagggt
     3541 atccccaatt agctcacgac cgccattgcc acgtaatatc agcgcacgtt tgcccgcaat
     3601 attttgtaat tcaggtaatt gtagcaagac ttcgctgatt tcccgatcct gtgggtagag
     3661 aatcttctgt ccgcttacgg tatgtagcgc cagtgcggtg gtgcgtccaa tggcgaaata
     3721 agtaggtagt tggggccatt tacgatcttg ctgatgcagc tgtgattggg caaaagcaac
     3781 cgcgtgttgc gagagggcaa acaacagatc gctctccccc agcgctgcca gttgatcagc
     3841 aagttgcggt aattgtcgac ccggagaaaa ctcaatcagc ggaaaatgcc aggccacctg
     3901 ccccagtgtg cgcagacggc tcactaactc ttctccagcg ggagacgggc gggtgaccag
     3961 gatactcatg ccggggcctc tccgttatag acttcagcga ggatctcgcg cgcgccgtta
     4021 ttcagtagct cttctgccag cgaaatcccc atttgttcgg catcttgcgg cgcaccgcgg
     4081 cgttcaccgc gaataatctg cgaaccgtcc ggcgcgccga ccagcgcaca tagccagatt
     4141 tcgccatcaa taagctcggc gtagctacca attggcacct gacatccgcc ttcgagacgg
     4201 gtattcatgg cgcgttctgc ggtaacgcgc agtgcagttt cgtggtgatt cagcgcggca
     4261 agcagctcgc gagtgcgtgt atcatcaagg cggcattcaa taccgactgc accttgtcct
     4321 accgccggaa gagaaatctc gggtggtaac gcggcgcgaa tgcgggactc cagacctaaa
     4381 cgttttagtc cggctacggc aagaatgatg gcatcgtatt cgccgttatc tagtttgctc
     4441 aggcgagtgc cgacgttgcc gcgcagggag cggataatca gatccgggcg gcgttcagcc
     4501 agttggcact ggcgacgtaa actggacgtc ccgacgatac tgcctgctgg taacgcatcc
     4561 agactgtcat agttattgga cacaaaggca tcgcgaggat cttcacgctc gcaaatagtg
     4621 accagcccca gaccttgcgg gaattcaacc ggcacatctt tcattgaatg tacggcgata
     4681 tcagcgcgat tctccaggag cgcgacttcc agctctttta caaataagcc ttttccgcct
     4741 actttcgcca gcggcgtatc aagaatcaca tcgccgcgcg tcaccatcgg taccagttca
     4801 acgaccaggc ccggatggct cgccatcaac ttgtctttga catagtgtgc ctgccagagt
     4861 gcaagtgggc tttggcgtgt ggcaattctt aaaacattgt ctaacatgct tgttaccgtc
     4921 attatcatcc gtggtccatc ctaacatcct tgccagagtg atgtcagtgt tgtggtgaaa
     4981 cgtagacgcc tgcgcaaacc gtaaaatgag gtctggcagt ggatcctgac aggcgtttca
     5041 cgccgtcgta ataaggaatt tacagagaat aaacggtgct acacttgtat gtagcgcatc
     5101 tttctttacg gtcaatcagc aaggtgttaa attgatcacg ttttagacca ttttttcgtc
     5161 gtgaaactaa aaaaaccagg cgcgaaaagt ggtaacggtt acctttgaca tacgaaatat
     5221 cccgaatgcc gcgtgttacc gttgatgttg gcggaatcac agtcatgacg ggtagcaaat
     5281 caggcgatac gtcttgtacc tctatattga gactctgaaa cagagactgg atgccataaa
     5341 tcaattgcgt gtggatcgcg cgcttgctgc tatggggcct gcattccaac aggtctacag
     5401 tctactgccg acattgttgc actatcacca tccgcgaatg ccgggttacc ttgatggtaa
     5461 cgttcccaaa ggcatttgcc tttacacgcc tgatgaaact caacgccact acctgaacga
     5521 gcttgaactg tatcgtggaa tgtcagtaca ggatccaccg aaaggtgagc ttccaattac
     5581 tggtgtatac accatgggca gcacctcgtc cgtagggcaa agttgttcct ctgacctgga
     5641 tatctgggtc tgtcatcaat cctggctcga tagcgaagag cgccagttac tacaacgtaa
     5701 atgcagcctg ctggaaagct gggccgcctc gctgggtgtg gaagtcagct tcttcctgat
     5761 tgatgaaaac cgcttccgtc ataatgaaag cggcagcctg gggggcgaag attgtggctc
     5821 cacccagcat atactgctgc ttgacgaatt ttatcgtact gccgtgcgtc tcgccggtaa
     5881 gcgtattctg tggaatatgg tgccgtgcga cgaagaagag cattacgacg actatgtgat
     5941 gacgctttac gcgcagggcg tgctgacgcc aaatgaatgg ctggatctcg gtggcttaag
     6001 ctcgctttct gctgaagagt actttggtgc cagcctttgg cagctctaca agagtatcga
     6061 ttccccatac aaagcggtgc tgaaaacact gctgctggaa gcctattcct gggaataccc
     6121 gaacccacgt ctgctggcga aagatatcaa acagcgtttg cacgacggcg agattgtatc
     6181 gtttggcctc gatccatact gcatgatgct ggagcgtgtt actgaatacc tgacggcgat
     6241 tgaagatttc acccgtctgg atttagtacg tcgctgcttc tatttaaaag tgtgcgaaaa
     6301 actcagccgt gaacgcgcct gtgtaggctg gcgtcgcgca gtgttgagcc agttagtgag
     6361 cgagtggggt tgggacgaag ctcgtctggc aatgctcgat aaccgtgcta actggaagat
     6421 tgatcaggtg cgtgaggcgc acaacgagct gctcgacgcg atgatgcaga gctaccgtaa
     6481 tctgatccgc tttgcgcgtc gcaataacct tagcgtctcc gccagtccgc aggatatcgg
     6541 cgtactgacg cgtaaactgt atgccgcgtt tgaagcattg ccaggtaaag tgacgctggt
     6601 aaacccgcag atttcacccg atctctcgga accgaatctg acctttattt atgtgccgcc
     6661 gggccgggct aaccgttcag gttggtatct gtataaccgc gcgccaaata ttgagtcgat
     6721 catcagccat cagccgctgg aatataaccg ttacctgaat aaactggtgg cgtgggcatg
     6781 gtttaacggc ctgctgacct cgcgcacccg tttgtatatt aaaggtaacg gcattgtcga
     6841 tttgcctaag ttgcaggaga tggtcgccga cgtgtcgcac catttcccgc tgcgcttacc
     6901 tgcaccgaca ccgaaggcgc tctacagccc gtgtgagatt cgccatctgg cgattatcgt
     6961 taacctggaa tatgacccga cagctgcgtt ccgcaatcag gtggtgcatt ttgatttccg
     7021 taagctggat gtcttcagct ttggcgagaa tcaaaattgc ctggtaggta gcgttgacct
     7081 gctgtaccgc aactcgtgga acgaagtgcg tacgctgcac ttcaacggcg agcaatcgat
     7141 gatcgaagcc ctgaaaacta ttctcggcaa aatgcatcag gacgccgcac cgccagatag
     7201 cgtggaagtc ttctgttata gccagcatct gcgcggctta atccgtactc gcgtgcagca
     7261 actggtttct gagtgtattg aactgcgcct ttccagcacc cgccaggaaa ccgggcgttt
     7321 caaggcgctg cgcgtttctg gtcaaacctg gggcttgttc ttcgaacgcc tgaatgtatc
     7381 ggtacagaag ctggagaacg ccatcgagtt ttatggcgct atttcgcata acaaactgca
     7441 cggcttgtca gtgcaggttg aaaccaatca cgtcaaatta ccggcggtgg tggacggctt
     7501 tgccagcgaa gggatcatcc agttcttttt cgaagaaacg caagacgaga atggctttaa
     7561 tatctacatt ctcgacgaaa gcaaccgggt tgaggtgtat caccactgcg aaggcagcaa
     7621 agaggagctg gtacgtgacg tcagtcgctt ctactcgtca tcgcacgacc gcttcaccta
     7681 cggctcaagc ttcatcaact tcaacctgcc gcagttctat cagattgtga aggttgatgg
     7741 tcgcgaacag gtgattccgt tccgaacaaa atcgattggc aacctgccgc ccgccaatca
     7801 ggatcacgat acgccgctat tacagcagta tttttcgtga tgagcgtgcc ggaaagcgag
     7861 gcttatccgg catgcaatct tagcggaaac tgactgtttc acccgcctgt tgcgtcgccg
     7921 cctgttccag caaatcccag aaggtttcgc cgctgcgatc gcaaatccat tcatcgcctt
     7981 tcaggtcaaa atggtagccg ccctgtttgg ttgccagcca cacctggtgc agcggctcct
     8041 ggcggttaat aatgattttg ctgccgtttt caaaggtaat ggtcagtacg ccgccgttga
     8101 tttcgcagtc gatatcgctg tcgccatccc tgtcgtccag acgctcttca atggtcagcc
     8161 acagttgatc agccaggcga tgaaattcac tgtcgttcat tgttgtatcc tgtttttaag
     8221 tgatggcggc agtatagcgg catggggtca gggcttcaaa gtttgcacct ctgctgctgc
     8281 gttccggcat gattcatccg tcaccggaat aatgatgtct ctgttttgaa cttcacgtac
     8341 gttctcctct ctgacacgag ttttcgccgg gcagggggag ggcgtaatcc ttacctctgt
     8401 atcggtccat gtataaacat tgcgatcgtc ataagcgaag cgatcgtttt tagttagcga
     8461 accgttaact ggaataattt gcaagctctt tgaccgtaat tggcggataa agtaagcatg
     8521 ctgtttgccg ctattaaatg agcgatcaat gaccaggtct ggcgtatcca gcgggattat
     8581 ctcaagctgg tcttcaccaa atgtcgataa ttttgttaca tacagccgat ctttatattg
     8641 cgcaatattg ccagtaatca gatggaatt
//
LOCUS       DAJTSF010000142         8491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000142 DAJTSF010000000
VERSION     DAJTSF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.142"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            44..397
                     /locus_tag="OO335_003643"
     CDS             44..397
                     /locus_tag="OO335_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6003874.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(434..2512)
                     /gene="flhA"
                     /locus_tag="OO335_003644"
     CDS             complement(434..2512)
                     /gene="flhA"
                     /locus_tag="OO335_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR6003875.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="OO335_003645"
     CDS             complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="OO335_003645"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6003876.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGTIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="OO335_003646"
     CDS             complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="OO335_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6003877.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="OO335_003647"
     CDS             complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="OO335_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6003878.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="OO335_003648"
     CDS             complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="OO335_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6003879.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="OO335_003649"
     CDS             complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="OO335_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6003880.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6415..6876
                     /gene="hycA"
                     /locus_tag="OO335_003650"
     CDS             6415..6876
                     /gene="hycA"
                     /locus_tag="OO335_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6003881.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7001..7612
                     /gene="hycB"
                     /locus_tag="OO335_003651"
     CDS             7001..7612
                     /gene="hycB"
                     /locus_tag="OO335_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6003882.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7649..>8491
                     /locus_tag="OO335_003652"
     CDS             7649..>8491
                     /locus_tag="OO335_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6003883.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENI"
BASE COUNT         1903 a         2305 c         2285 g         1998 t
ORIGIN      
        1 attaagatgt aatataaatg catcttaatg aggtggaggc agcatgtccg gcaagcgtat
       61 ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca
      121 ggcgtcagcg gagccggagc attacgaggc gttgtttgtt tacgcgcaaa agcggctgga
      181 taaatgtgtg ttcggcgaag agaaaccagc ctgtaagcag tgtccggtgc actgttacca
      241 gcctgcaaag cgtgaagaga tgaaacagat tatgcgttgg gccgggccga gaatgctgtg
      301 gcgtcatccg atcttaaccg tgcgtcatct catcgatgac aagcgtccgg tgccagaact
      361 gccggaaaaa tatcgtccga aaaaacctcg tgaatagtat gccggacagg cagatctgtc
      421 cggcaatttg cagttaaatc aatgccgatt tatcaattcc cagccgcttc atccgtgaca
      481 gcagggtcgt gcgtttcagc cccagacgtt gcgcagcgcc tttaggcccg gcaaccacgc
      541 tgttggtttc tttcagcacg cgcacaatca actgatattc atcttcgccc tcctgggcga
      601 caaccgttgc ggcaggcggc gtttcaggct ccggtaaagc aatatctggc aatgacagct
      661 gcagcacgtt gccgcgtgtt agcaatactg cgcgctcaat gacgttttcc agttcgcgta
      721 cgttacccgg ccactccatg ttgctcaagg tgcgcagcgt ctcggcagga atgctgtcga
      781 tattgcgccc cagacgacgg gcaattttga aggtaaaggc tttcgccagc agcggaatat
      841 cttccggacg ctcgcgtagt ggcggcaggt gaatcgggaa tacgttcagg cggtaataga
      901 gatcgctacg gaactctcgg tcggcgacca tttttttcag atcgcggtta gtcgcggcaa
      961 ttaaacgtac gtccgtctga atgattttgt tgctgccgag acgttcaaac tcctgttcct
     1021 gcaatacacg cagcaacttc ggctgtaact ccagtggcat atcgcccact tcgtcgagga
     1081 acagagagct tttatccgcc agttcaaaac gaccaatgcg ctgggcgttg gcaccggtaa
     1141 aagccccacg ctcatgacca aacagatcgc tttccagcaa tccggcaggc atcgccgcgc
     1201 agttcatttt gaccatgcgg cgattattac gcccactgag attatggatc gcacgggcaa
     1261 tcagctcttt acccgagcca gtttcaccga ggatcagcac ggtactgtca ctttgcgcca
     1321 ccatttcaac ttgtttaagc acgctgtaca tggcttcgct gcggccaata atctcgccaa
     1381 attcactatc aacattgttg agctgctcgg tcagggcgag gttttcatca accagccgtt
     1441 ctttcagacg atggatttcc tgataggcga gggcgttatc gacagcgatt gccacacgtt
     1501 cggcaatctg gcgcagcaaa ttcagattgg tagtggtaaa cactttctct tcgcattgcg
     1561 ccagtttcag cacgcccagc atggtgtcgc cagacatcag cggtaacagg cacaaggttt
     1621 gaatctggtt gccccaggtg tcgaacaaca tgcgttcata gggggctaaa tcgtcccgct
     1681 cgtagagatt gatcagcagc atctctttac ttttgaacac gcgttcggtg agggttccgg
     1741 cttcatcgac ttcgctctgt tcgtgggcgg gatgttgttt atcaagatag tgagtggagt
     1801 agatgttgag tttgttttta cggtggctgc gtaagacaat gctgatatcg tcaatgtcga
     1861 aatagtaatg gatttctttg gcgacttcgc tgaccagttc gtccatatcc aggcgggaaa
     1921 gcaccgcgtt ggtaatggca accaggatgc ggaagttatc gcgctcccgg cataacaact
     1981 catagtcgac attgttgacg acgcggctct ggatttgttc ggtgacgaca gaaacgatct
     2041 gcgtaaatgt ttgcagacga ttgaactctt tttcgctcca gggacgctcg tcataacgaa
     2101 taaattcaca gccaccaaaa atatgccctc ccgccgccag tggcatcagg caatagtgac
     2161 caaatttggg gtatagccca cctgcggcca gctgcggcca ggtttcacaa aattcttcgt
     2221 aactgcaatg cagcgtatca gggcgcgaca aaatgctgcg taccggaccg tgtgccagaa
     2281 cagtttcgtc ttcatattta atgggggtgt ctttttcacg cgacgcgtaa taagacgcac
     2341 gttgagtctg cgcttgccac aacacaatag ccgcgttgtc ggcgagcgca gaacgcttta
     2401 ccagttgcga aagagcctca cacagcgagg ccagatcggg ctgctgcaat agtgtccgag
     2461 tgatgtcgaa caacccttgc tgtccgagat cactcatcgg tgtatatgac attttgttct
     2521 tccaggaaag caccgcaaag cggtgcaaaa ggtcattgac taactatagg agatttagaa
     2581 ttttattagc atatacgcgg aagcggttcg gcgtgtggta aatcgagggt tcgtttcacg
     2641 ccatacagac cggcaagacg aacaccttta cgttccacca cttcaccaat cagcgccgcg
     2701 tctttcccca gtggatggga atgtaacgct gccagcactt gctctgccgc gttgcgttca
     2761 acggcgatca ccagtttgcc ttcgttggca aagttcaggg cgtccagtcc cagcaattcg
     2821 caaacgccgc gcacggcagg tttaaccggc agcgctgatt cagaaatttc aataccacaa
     2881 ccgcaggctg ccgcgaactc atgaaccacc gcgtttacac caccacgggt ggcatcacgc
     2941 agcgctttca cgccgggaat gtcacgcagc gtctgaataa gcggcgtcag caccgcgcag
     3001 tcgctgacca gttcgccatc cagccccagc tgctcacgca ggttaaggat agtcgccccg
     3061 tggtcgccga gtgtaccgct aaccagcaat acatcgcctg cggttagcgt ctgtgcgccc
     3121 cagtgaatat tcgccggaat tgtgcccatg ccagcggtgt tgataaacag tttatctgcc
     3181 gcgccgcgct gcaccacttt agtatcgcca gtaacgatgg caatgcctgc cgtgcgggcg
     3241 gtttctgcca tgctggtcac tacggctttc agtgtctcca tcggcaatcc ttcttcgagg
     3301 ataaagccac aggagagata gcgcggaata gcgccactgc ccgcaacgtc attcgcggtg
     3361 ccgcaaatcg ccagcttgcc gatattaccg ccaggaaaga acagcgggtc aatgacgtaa
     3421 ctgtcggtgg agaacgccag acggtcgcct tccgctacca gctgcgccag atcaagacgt
     3481 gcctgatctt cctgctctgc cagccacggg ttggcaaagg cttccataaa caggctgttg
     3541 attaattgct gcatcgcctg gccgccgctg ccgtgggcga gttggatatt attcacgctt
     3601 cactctcctg ctgacgatac tgataccacg cggcgcacgc tccttcggag gagaccatca
     3661 gtgcaccaaa tgcggtttga ggattacagg tgttaccaaa cagcgggcat tgatgcggct
     3721 tacatttgcc cgttaatacc tcaccacaac gcgcgcgcgg gtcatcgcag acctgctgcg
     3781 gtgccgggcg gaaatgtgct tcggcatcga atcgttgata atccggcgtc aggtgcacgc
     3841 cagaagattc aatcacgcct aagccgcgcc attcgctgtc gccgttgaca cagaacacat
     3901 cggcaatcgc ctgttgcgcc agtaggttac cggcatccgg taccacccga cgatactgat
     3961 tctctacctt gctgtgggcc gctattttct gctccaccag catgaccacg ccttgcagta
     4021 gatcaagggg ttcgaaacca gcgaccacca gcggacgatg aaaatcgctg gcgataaaat
     4081 tataggcatc agtgccgata accatactaa cgtggcccgg cgcgaggaac gcgtcgatac
     4141 cgttatccgg ctgttccagc aaactgcgca gcgtcgggat aagcgtaatg tgctggcaga
     4201 agaagtaaaa attctgcaca tcacgcgctt tcgcctgttg cagagtgatg gcggtggtcg
     4261 gcatggtggt ttcaaaacct aagccgaaga acaccacttt gcgggttgga ttctcctgcg
     4321 ccagtttcaa cgcatccatc ggcgagtaaa cgatgcgcac atcggcaccg cgtgcttttg
     4381 cctgcaacag cgatccctgt ttccctggta cgcgcatggc gtcgccaaag gtacagaaga
     4441 tgacttccgg atggctggca atctccacgc aggtgtcgat tctacccatc ggcagtacgc
     4501 acaccgggca acccggaccg tggataaact caacgttttc aggcagtaac tggtcgaggc
     4561 cgaatttaaa aatggcgtgg gtatgaccgc cacacacttc cataatccgc agagggcgtt
     4621 cggcggtgta agagagatgt gaagcacgtt cgcgcagatg ctcaattaac tgcatcacct
     4681 gttccggcgc gcgatattca tcaacaaaac gcattatttt tcctcgccat acaacagcgc
     4741 gccgacatcc ggctcaacgt caaacatgtt ttgtaaggcg tcgagagtgt cgcgtgcttc
     4801 ggcttcatta attacgctca tggcaaagcc aacgtgaacc agtacccact ggcccacgcg
     4861 cggctgaccg ttttcatcgc agctgccgac taacgttaaa tcgacatcgc gctgaatgcc
     4921 gcagacgtcg actttcgcct ggttgccgtc aatggtgcgg atctggccgg gaacgcctat
     4981 gcacatcgct gtgtctccag ccagttcagc cactggtcca tcccttcgcc gctggtggcg
     5041 gaaataagga tgatttcaat ttctggattg acttcgcggg cgcaggcgat gcacttctca
     5101 acgtcaaagt tgagatacgg caacaggtca actttgttga gcagcatcag cgaggcggcg
     5161 gcaaacatat gcggatattt cagcggtttg tcttcacctt cggtaacgga aagcaccgct
     5221 actttgtgtt tttcaccgag atcgaagctg gccgggcata cgaggttgcc aacgttttcg
     5281 ataaacagaa taccgttatc gtccagtggc agacgcggtg cggcgtcggc aatcatctgt
     5341 gcgtcaagat ggcagccttt accggtgttc acctgaatcg ctggtgtacc ggtagcgcga
     5401 atgcgtgcgg catcgttcac ggtttgctgg tcgccttcaa taaccgcgca cggaacgctg
     5461 tctttcaggc gcattaaggt ttccgtcagc agggtggttt taccggaacc agggctggaa
     5521 accaggttga gcaccagttg cttgcgggca gcaaagcgcg cgcggttgcg ttcagccaga
     5581 cggttgtttt tgtccagcac gtcaatttcg acttccagca tccgacgctg gctcatgccc
     5641 ggtgtgtgag tgcccgcttc gccatgaccg taatgcaggt cgccttcttc agtctggctg
     5701 ggggtaaatt caggcgcttt aatgccggtg attttcatct gcgggcgtgc cgccggggca
     5761 aatggcgcgc tacgaaacgc ggaatgaggg ttatgttcat caccctcgat atacaggttg
     5821 ccttcaccgc aaccgcatgt tgtacacatc gctcactcct ggtctatttc tatccgccga
     5881 atctgtaaac cgtcgtctgc cacaatctgt agcatgtcac catgacactg tggacagcgg
     5941 cggacgcgct gggtcagtag cgtcacatac tgttggcatg tttcacacca gcattcggcc
     6001 tcttgttctt cgaggtgcag tttacaacct tccgccacgc tgccgcggca aaccagatca
     6061 aaacaaaagg caagagagct ggtttcgaca caagaaaatg cgccaatttt gagccagacc
     6121 ccagttacgc gttttgcgcc gtgttttgcg gcctgctgtt cgatcaattc cagtgcccgt
     6181 tggcagaggg ttatttcgtg catatcgcct cccattaact attgccagct acaagcaata
     6241 attgcgccag tgttgattat ccctgcggtg aataatgtcg atgatgtcga aatgacacgt
     6301 cgacacggcg acgaaattca tctttagctt aaaaatctct ttaataacaa taaattaaaa
     6361 gttggcacaa aaaatgctta aagctggcat ctctgttaaa cgagtaacct gacaatgact
     6421 atttgggaaa taagcgagaa agccgattac atcgcacagc ggcatcgtcg cctacaggac
     6481 cagtggcaca tctactgcaa ttcgctggtt caggggatca cgttatcgaa agcgcgcctg
     6541 catcacgcca tgagctgcgc gccggacaaa gaactctgtt tcgtcctttt tgaacatttt
     6601 cgtatttacg tcaccctggc ggatggcttt aacagccaca ccatcgagta ttacgtcgaa
     6661 acaaaagatg gcgaagacaa acagcggatt gcgcaggcgc aactgagcat tgacggcatg
     6721 attgatggca aggtcaacat ccgcgatcgc gaacaggttc tggaacacta tctcgaaaaa
     6781 atcgctggcg tttacgacag cttatacacc gccattgaaa acaatgtgcc ggtgaattta
     6841 agccaactgg taaagggaca aagcccggca gcatgagctg aggctttgcc cgttttgcag
     6901 gcgttacgcc tgtttgggga tgggcgtgtc gatgagtgtc gaaaatgaca tttcatcggc
     6961 atgttttcgt caaaaatgac aatcacctga ggaatgcctg gtgaatcgtt ttgtaattgc
     7021 cgactccacg ctctgtatcg gctgccacac ctgtgaggcc gcctgttcag agacgcatcg
     7081 ccagcacggc ctgcaatcaa tgccgcgcct gagagtgatg ctcaatgaaa aagaatctgc
     7141 gccgcagctc tgtcaccact gtgaagatgc accctgcgcg gtggtctgcc cggttaacgc
     7201 catcacccgc gttgatgggg ccgtgcagtt gaatgaaagt ctgtgcgtaa gctgcaaact
     7261 gtgcggcatc gcctgcccgt ttggcgcaat tgaattttcc ggcagccgtc cgctggatat
     7321 tccggcaaac gccaataccc cgaaagcgcc accggcaccg cctgctccgg cgcgtgtcag
     7381 cacattgctt gactgggtgc caggtattcg cgcgatcgcc gtcaaatgtg acctttgtag
     7441 ctttgatgaa caaggtccgg cctgtgtgcg gatgtgcccg actaaagccc tgcatctggt
     7501 ggataacacc gatatcgccc gcgtcagcaa acgtaagcgt gagctgacct ttaacacgga
     7561 ctttggcgat ctcaccttgt ttcagcaggc tcaaagtgga gaggctaaat gagcgcaatt
     7621 tccctgatca atagcggcgt ggcatggttt gtcgccgccg ctgttctggc atttctcttt
     7681 tcaaaaagcg ctaagtggct ggatagccgg aattggcggc gcggttggta gtctgtatac
     7741 ggcagccgcg ggcttcactg tactgactgg cgcggttggc gtgagcggtg cgctgtcgct
     7801 ggtaagctac gatgtgcaaa tctccccgct taacgcgatt tggctgatta ccctcggcct
     7861 ttgcggtctg tttgtcagcc tctataacat tgactggcat cgccacgcgc aggtgaagtg
     7921 caacggcttg cagatcaata tgttgatggc tgccgccgtc tgcgccgtca tcgccagcaa
     7981 cctcggcatg ttcgtggtaa tggccgaagt catggccttg tgtgcggtgt tcctcaccag
     8041 caacagcaaa gagggcaaac tgtggtttgc gctgaggcgt cttggcactc tgctgctggt
     8101 gattgcttgc tggctgctgt ggcagcgtta tggcacgctg gatctgcgcc tgctggatat
     8161 gcgtatgcaa cagctgccgc tcggttccga tatctggctg ctcggtgtga ttggctttgg
     8221 cctgctggct gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc
     8281 tgcgccagct gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt
     8341 aaccctgtca ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct
     8401 cggcatgatc accgcgttcg tcggtggtct gtatgcgttg atggaacaca acatccagcg
     8461 cctgctggct taccacaccc tggaaaatat c
//
LOCUS       DAJTSF010000143         8441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000143 DAJTSF010000000
VERSION     DAJTSF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.143"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..188
                     /gene="csgC"
                     /locus_tag="OO335_003653"
     CDS             <1..188
                     /gene="csgC"
                     /locus_tag="OO335_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR6003884.1"
                     /translation="SSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSD
                     GQALHLSQQWTPSSEKS"
     gene            309..620
                     /locus_tag="OO335_003654"
     CDS             309..620
                     /locus_tag="OO335_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6003885.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            715..1248
                     /gene="ymdB"
                     /locus_tag="OO335_003655"
     CDS             715..1248
                     /gene="ymdB"
                     /locus_tag="OO335_003655"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6003886.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1190..2671
                     /gene="clsC"
                     /locus_tag="OO335_003656"
     CDS             1190..2671
                     /gene="clsC"
                     /locus_tag="OO335_003656"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR6003887.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2679..3836)
                     /gene="mdoC"
                     /locus_tag="OO335_003657"
     CDS             complement(2679..3836)
                     /gene="mdoC"
                     /locus_tag="OO335_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6003888.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4230..5765
                     /gene="mdoG"
                     /locus_tag="OO335_003658"
     CDS             4230..5765
                     /gene="mdoG"
                     /locus_tag="OO335_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6003889.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5758..8301
                     /gene="mdoH"
                     /locus_tag="OO335_003659"
     CDS             5758..8301
                     /gene="mdoH"
                     /locus_tag="OO335_003659"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6003890.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2136 a         2001 c         2200 g         2104 t
ORIGIN      
        1 gcagttcagg gcaaagtcag acgaagcaag aaaagaccct ctcattgcct gctaatcaac
       61 ccattgcttt gacgaagttg agtttaaata tttccccgga agatcgggtg aaaatagttg
      121 ttactgtttc tgatggacag gcacttcatt tatcacaaca atggacgccc tcttcagaaa
      181 agtcttaatt tgttgaaata tcgagcataa gatgaatctg gagagaatgg tctgctgcga
      241 atcagccaac ctgaaagtat ggataacaca accttcaagg atgactaatc attgaggaaa
      301 tagaataaat gttcagacct tttttagact ctcttatgct cggcagtttg ttttttcctt
      361 ttattgccat tgcaggaagc accgcgcaag ggggcgtgat ccatttttat ggccaaattg
      421 tggaaccggc atgtgacgtc agcacccagt catcacccgt agaaatgaac tgcccacaga
      481 atggttctat tccgggcaaa acctactcca gcaaagcgtt aatgagcggc aatgtcaaaa
      541 acgcgcaaat agcctcagta aaagtgcagt atcttgataa acagaaaaag ctggctgtaa
      601 tgaacatcga atataactaa gtttctgaac aactcacgtt gctgagcaga aaaatgcgat
      661 ttaaccaaaa agcctgctgt acacttaaga aacaagaagg tgtaaggagg cgttatgaaa
      721 acgcgtattc atgttgtgca gggtgatatt accaaactgg ccgttgatgt gattgtgaat
      781 gcggctaatc cgtcattaat gggaggcggc ggcgtcgatg gggccattca tcgcgcagcg
      841 ggtccggccc tgctggatgc ctgtttaaaa gtcaggcaac agcagggcga ttgccctacg
      901 gggcatgccg ttattacgct tgcaggcgat cttcccgcta aagccgtagt acacaccgtc
      961 gggccagtct ggcgtggtgg ggaacaaaac gaagaccagc ttttgcagga tgcctatctg
     1021 aatagcctac gactggtggc ggcaaacagc tatacgtcag tggcttttcc tgcaatcagt
     1081 actggggttt atagttaccc tcgtgcggca gcggctgaaa tcgcagttaa aaccgtttca
     1141 gaatttatta cccgtcacgc tttacccgaa caggtatact ttgtctgtta tgatgaagaa
     1201 aacgcccacc tctacgaaag actccttacc caacaaggag atgaatgatt tgccccggct
     1261 ggcgagcgcg gtgctgccac tgtgttcgca acatcccggt cagtgtggtc ttttccctct
     1321 ggagaaaagt ctggatgcgt ttgccgcccg gtatcgtctg gccgaaatgg cagagcatac
     1381 gctggatgtt cagtattaca tctggcagga cgatatgtcg gggcggttac tgttttccgc
     1441 cctgttagcc gcagcaaagc gtggcgttcg cgtccgtttg ttgctggacg acaacaatac
     1501 gcccggactt gacgacattt tgcgcttgct tgacagtcat ccacgcattg aagtccggct
     1561 ttttaatcct ttctcgtttc gcttgctacg tccgcttgga tatatcaccg acttttcccg
     1621 tctcaatcgc cggatgcaca ataaaagttt cactgtcgat ggcgtggtta ccctagtggg
     1681 aggacgaaat attggtgatg cctattttgg tgcaggggaa gagccacttt tttcggattt
     1741 agatgtcatg gcaataggcc ccgtggtaga ggatgttgcc gatgatttcg cccgctactg
     1801 gtattgcaaa tcggtttcac ccttacagca ggtgctggat gtcccggaag gtgaaatggc
     1861 ggatcgcatc gagttacccg cctcctggca taacgatgcc atgacgcatc gttatttacg
     1921 caaaatggaa tccagtccat ttataaatca tctggttgat ggaacgttgc cgcttatctg
     1981 ggcgaagaca cgtttattaa gtgatgatcc ggcgaaaggg gagggcaagg caaaacggca
     2041 ttcactgtta ccgcagcgcc tgttcgatat catgggctca cccagtgaac gcatcgatat
     2101 tatctcttcc tattttgtac cgacacgcgc aggtgtggcg caactcttac ggatggtgag
     2161 aaaaggggta aagattgcga tcctaaccaa ttctcttgcc gctaacgatg ttgctgtcgt
     2221 ccatgccgga tacgcgcgct ggcgcaaaaa attgctccgc tatggcgtgg aattatatga
     2281 actcaagccg acgcgtgaac aaagcagtac gttacacgat cgcggcataa ccggtaattc
     2341 cggtgccagc ctgcatgcta aaacttttag catcgatggt aaaacggtgt ttatcggctc
     2401 tttcaatttc gatccgcgtt caacattgct caatactgaa atgggcttcg tgatagagag
     2461 cgaaacgctg gcacagttaa ttgataaacg ctttattcag agccagtacg atgcggcctg
     2521 gcagctccgt ctggacaggt ggggacggat caactgggtt gatcgtcatg caaagaaaga
     2581 ggttattctc aaaaaagaac ccgccaccag tttctggaag cgggttatgg tcagactggc
     2641 gtcgatattg cccgtggaat ggttattgta aatgtggctt aacgggctgg tgctttatcg
     2701 ttctcacgct taacaaccgg tttaccagag aacaaaaact tcagtaacgg gatgcgtaaa
     2761 tgaatttcat acagaattat cgcaatccct actacgaata tcaggccaca gagaaaacca
     2821 agccagttgg aggtgatgtg cggtgtaatg tatgcgccga aaaacagcgt taacgggtgg
     2881 tgaaccagat agataaacag cgatgcgtta acaaaataag tcacccgcgc tgactggaag
     2941 ttaagcaaac ggtggccaaa ggagaagacc acattcacca tccacaggcc gaggaccatg
     3001 gtgatcaccg actcggtttc gtacatccag gcatcgccac tgccatagcg ctggttgagt
     3061 aaataagcga caaacgccaa tgctgcagcg agggtacagc cacgagacgg cgtggtaaac
     3121 aaggctttaa gatgagggaa aatgaaagcc agtgcgccga ggataaagaa cggtaaataa
     3181 aatagcgttt gcatgacaat aaaattgaac atgccattac tcagaatggg tggatacaca
     3241 ataaaaatcg ttcttcttat taccgcatag ccgatgccga ggcataaaaa aatcaccgat
     3301 agttttacca tcgagaattt tttattcgtt ttatcagaat tttctaaatt atttctgatg
     3361 cgcttaaata tccatacaca cagcgtcgtc atgactacta acaccagtaa aaaccacagg
     3421 tgtgatatta attcccaggc caacgtatta tatttgtcat acaatgatag cccaggccaa
     3481 ctttccgctt tccctttgac atattgcagc ataataaatt gcggtaatgt cagtaggggg
     3541 atggctgtta acatcgggat acctacacgt tcgacacgta ctttccacca ttttttcaag
     3601 ggatagcgta aaaaaagcat gtaggaaaag tatccggata taacgaaaaa tacctgcatg
     3661 cggaacgagt ggatgaagtc attaaaaagg gttagccaca atgacggttc ggcgctattc
     3721 acatgccatg tatggctcga atagattaaa gaaatatgaa aagggatccc taacaacatc
     3781 agccaggcgc ggatggagtc gaggaaatat tcacgttgcg cgggtactgg attcatatat
     3841 ggttaactaa tctcggattt ttcgtcttat ccctgtcggg ttatgccttt aggcttgttg
     3901 ccatagcgac accgacctga ccgcgccagg cgcaggcttc aaggttttta tgcatagcat
     3961 catcgctacc actaaccaga atggaagcgt ctgtaagacg gttgataaat aaatttgctg
     4021 gcaaacccta cacgaagtcg atgcttctgt ctttaggaga agcacggaaa gtgaaaacgg
     4081 ttgcaatcag gtgcttaatc catgagccag cgtgctgaac gataccggga ttcagttgtc
     4141 ggaatggctg gttatccatt aaaatagatc ggatcgatat aagcacacaa agggggaagt
     4201 gcttactaat tatgaaacat aaactacaaa tgatgaaaat gcgttggttg agtgctgcag
     4261 taatgttaac cctgtataca tcttcaagct gggctttcag tattgatgat gtcgcaaagc
     4321 aagctcaatc cttagccggg aaaggctatg aggcgcctaa aagcaacttg ccctccgttt
     4381 tccgcgacat gaaatacgcg gactatcagc agatccagtt taatcatgac aaagcgtact
     4441 ggaacaatct gaagacccca ttcaaactcg agttctacca tcagggtatg tacttcgata
     4501 ccccggtcaa aataaatgaa gtgactgcca ccgcagtcaa acgaatcaaa tacagcccgg
     4561 attatttcac tttcggcgat gttcagcatg acaaagacac ggtaaaagac cttggttttg
     4621 ccggttttaa agtgctttac ccgatcaaca gcaaagataa aaacgatgaa atcgtcagca
     4681 tgctcggggc cagctatttc cgcgtgattg gtgcaggtca ggtttatggc ctttctgccc
     4741 gcggcctagc aattgatacc gctttgccat cgggtgaaga atttccacgc ttcaaagagt
     4801 tctggatcga gcgtccaaaa ccgactgata aacgtttaac catttatgca ttgcttgact
     4861 cgccgcgtgc gacaggtgct tacaaattcg tggttatgcc agggcgtgac acggttgtgg
     4921 atgtgcagtc gaaaatctat ctgcgcgata aagtcggcaa actgggggtt gcaccgttaa
     4981 ccagtatgtt cctgtttggg ccgaaccaac cgtcgcctgc aaataactat cgtccggagt
     5041 tgcacgactc taacggtctg tctatccatg ctggtaatgg cgaatggatc tggcgtccgt
     5101 tgaataaccc gaaacattta gcggtcagca gcttctccat ggaaaacccg cgaggctttg
     5161 gtctgttgca acgaggtcgt gatttctccc gctttgaaga tctcgatgat cgttacgatc
     5221 tccgtccaag cgcatgggtg actccgaaag gggagtgggg caaaggcagc gttgagctgg
     5281 tggaaattcc aaccaacgat gaaaccaacg ataacatcgt cgcttactgg acgccggatc
     5341 agctgccgga gccgggtaaa gagatgaact ttaaatacac catcaccttc agccgtgatg
     5401 aagacaaact gcatgcgcca gataacgcat gggtgcaaca aacgcgtcgt tcaacggggg
     5461 atgtgaagca gtcgaacctg attcgccagc ctgacggtac tatcgccttt gtggtcgatt
     5521 ttaccggcgc agagatgaaa aaactgccag aggatacccc ggtcacagcg caaaccagca
     5581 ttggtgataa tggtgagata gttgaaagca cggtgcgcta taacccggtt accaaaggct
     5641 ggcgtctggt gatgcgtgtg aaagtgaaag atgccaagaa aatcactgaa atgcgtgctg
     5701 cgctggtgaa tgccgatcag acgttgagtg aaacctggag ctaccagtta cctgccaatg
     5761 aataagacaa ctgagtacat tgacgcaatg cccatcgccg caagcgagaa agcagcattg
     5821 ccgaagactg atatccgcgc cgttcatcag gcgctggatg ccgaacaccg cacctgggcg
     5881 cgggaggatg attccccgca aggctcggta aaggcgcgtc tggaacaagc ctggccagat
     5941 tcacttgctg atggacagtt aattaaagac gacgaagggc gcgatcagct taaggcgatg
     6001 ccagaagcaa aacgctcctc gatgtttccc gacccgtggc gtaccaaccc ggtaggccgt
     6061 ttctgggatc gcctgcgtgg acgcgatgtc acgccgcgct atctggctcg tttgaccaaa
     6121 gaagagcagg agagcgagca aaagtggcgt accgttggta ccatccgccg ttacattctg
     6181 ttgatcctga cgctcgcgca aactgttgtc gcgacctggt atatgaagac cattcttcct
     6241 tatcagggtt gggcgctgat taatcctatg gatatggttg gtcaggattt gtgggtttcc
     6301 tttatgcagc ttctgcctta tatgctgcaa accggtatcc tgatcctttt tgcggtactg
     6361 ttctgttggg tgtccgccgg attctggacg gcgttgatgg gcttcctgca actgcttatt
     6421 ggtcgcgata aatacagtat atctgcgtca acagttggcg atgaaccatt aaacccggag
     6481 catcgcacgg cgttgatcat gcctatctgt aacgaagacg tgaaccgtgt ttttgctggc
     6541 ttgcgtgcaa cgtgggaatc agtaaaagcc accgggaatg ccaaacactt tgatgtctac
     6601 attctgagtg acagttataa cccggatatc tgcgtcgcag agcaaaaagc ctggatggag
     6661 cttatcgctg aagtcggtgg cgaaggtcag attttctatc gccgccgccg tcgccgcgtg
     6721 aagcgtaaaa gcggtaatat cgatgacttc tgccgtcgct ggggcagcca gtacagctac
     6781 atggtggtgc tggatgctga ctcggtaatg accggtgatt gtttatgcgg cctggtgcgc
     6841 ttgatggaag ccaacccgaa cgccgggatc attcagtcgt cgccgaaagc gtccggtatg
     6901 gatacgctgt atgcgcgctg tcagcagttc gcgacccgcg tgtatgggcc actgtttaca
     6961 gccggtttgc acttctggca acttggcgag tcgcactact ggggacataa cgcgattatc
     7021 cgcgtgaaac catttatcga gcactgcgca ctggctccgc tgccgggcga aggttccttt
     7081 gccggttcaa tcctgtcaca tgacttcgta gaagcggcgt tgatgcgccg tgcaggttgg
     7141 ggggtctgga ttgcttacga tctcccgggt tcttatgaag aattgccgcc taacttgctt
     7201 gatgagctaa aacgtgaccg ccgctggtgc cacggtaacc tgatgaactt ccgtctgttc
     7261 ctggttaagg gtatgcaccc ggttcaccgt gcggtgttcc tgacgggcgt gatgtcttat
     7321 ctctccgctc cgctgtggtt tatgttcctc gcgctctcta ctgcattgca ggtagtgcat
     7381 gcgttgaccg aaccgcaata cttcctgcaa ccacggcagt tgttcccggt gtggccgcag
     7441 tggcgtcctg agctggcgat tgcacttttt gcttcgacca tggtgctgtt gttcctgccg
     7501 aagttattga gcattttgct tatctggtgc aaaggaacga aagaatacgg cggcttctgg
     7561 cgcgttacat tatcgttgct gctggaagtg ctgttttccg tgctgctggc tccggtacgc
     7621 atgctgttcc atacggtctt cgttgtcagc gcgttccttg gctgggaagt ggtgtggaat
     7681 tcaccgcagc gtgatgatga ctccacttca tggggcgaag cgttcaaacg ccacggctca
     7741 cagctgctgt tggggttagt gtgggctgtt gggatggcgt ggctggatct gcgtttcctg
     7801 ttctggctgg caccgattgt cttctcgttg atcctgtcac cgtttgtttc ggtgatttcc
     7861 agccgtgcca ccgttggtct gcgcaccaaa cgctggaaac tgttcctgat cccggaagag
     7921 tattcaccgc cgcaggtgct ggttgatacc gatcggttcc ttgagatgaa tcgccaacgc
     7981 tcccttgacg atggctttat gcacgcagtg tttaacccgt catttaacgc tctggcaacc
     8041 gcaatggcga cagcgcgtca ccgcgccagt aaggtgctgg aaatcgcccg tgaccgccac
     8101 gttgaacagg cgcttaacga gacgccagag aagctgaatc gcgatcgtcg cctggtgctg
     8161 ctaagcgatc cggtgacgat ggcccgtctg catttccgcg tctggaattc ccctgaaaga
     8221 tattcttcat gggtgagtta ttacgaaggg ataaagctca atccactggc attgcgtaaa
     8281 ccggatgcgg cttcgcaata aaaacgtagt tgcctgatgc gctacgctta tcaggcctac
     8341 atcgttcctg caatttattg attttgcaag attttgtagg tcggataagg cgttcacgcc
     8401 acatccggca aaaacaacga gccaataaaa ataccggcgt t
//
LOCUS       DAJTSF010000144         8377 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000144 DAJTSF010000000
VERSION     DAJTSF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.144"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..956
                     /gene="espL1"
                     /locus_tag="OO335_003660"
                     /pseudo
     CDS             1..956
                     /gene="espL1"
                     /locus_tag="OO335_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1128..1235
                     /locus_tag="OO335_003661"
     CDS             1128..1235
                     /locus_tag="OO335_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003891.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="OO335_003662"
     CDS             complement(1288..2046)
                     /locus_tag="OO335_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR6003892.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2333..3262
                     /gene="pfkB"
                     /locus_tag="OO335_003663"
     CDS             2333..3262
                     /gene="pfkB"
                     /locus_tag="OO335_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6003893.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3363..3653
                     /gene="ghoS"
                     /locus_tag="OO335_003664"
     CDS             3363..3653
                     /gene="ghoS"
                     /locus_tag="OO335_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6003894.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3759..4619
                     /locus_tag="OO335_003665"
     CDS             3759..4619
                     /locus_tag="OO335_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6003895.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4660..5196)
                     /locus_tag="OO335_003666"
     CDS             complement(4660..5196)
                     /locus_tag="OO335_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6003896.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5343..6011
                     /gene="hxpB"
                     /locus_tag="OO335_003667"
     CDS             5343..6011
                     /gene="hxpB"
                     /locus_tag="OO335_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR6003897.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6174..6764
                     /locus_tag="OO335_003668"
     CDS             6174..6764
                     /locus_tag="OO335_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6003898.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6897..8287
                     /gene="tcyP"
                     /locus_tag="OO335_003669"
                     /pseudo
     CDS             6897..8287
                     /gene="tcyP"
                     /locus_tag="OO335_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT         2172 a         2004 c         2008 g         2193 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttctagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattagcca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt
     2221 tatatatatt tatctgcaaa attttaaata aagctctaat aaatcatatt gttaatttct
     2281 tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg
     2341 tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta
     2401 tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa
     2461 cgtcgcccgc gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg
     2521 cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt
     2581 agaagccaaa gactggaccc ggcagaattt acacgtacat gtggaagcaa gcggtgaaca
     2641 gtatcgtttt gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga
     2701 gcaagttctg gaaattgaat ccggggccat cctggtcata agcggaagcc tgccgccagg
     2761 tgtgaagctg gaaaaattaa cccaactgat ttccgctgcg caaaaccaag ggatccgctg
     2821 catcatcgac agttctggcg aagcgttaag tgcagcactg gcgattggta acatcgagtt
     2881 ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccaga
     2941 cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt
     3001 ttcccttggt ccacaaggag cgctgggtgt cgatagtgaa aactgcattc aggtggtgcc
     3061 accaccggtg aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac
     3121 actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg
     3181 gagtgcagcc acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat
     3241 ttacgcttac ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa
     3301 cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa
     3361 ctatggccag tggcgatctt gtccattatg tcataaccgt catgttgcat gaggatacat
     3421 tgactgaaat taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga
     3481 cggatgatga gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc
     3541 aaagtgaaga agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag
     3601 atcctgaaat caccatcacg acctgggagg aatggaatag caacagaaaa taaatggttt
     3661 ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc
     3721 taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc
     3781 ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag
     3841 aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg
     3901 aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta
     3961 gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc
     4021 tggtgatgga ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc
     4081 agcaaattgc gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata
     4141 actcgctctc cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg
     4201 ctgaacaacg gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca
     4261 atatcgacgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa ccgcagcctt
     4321 ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca
     4381 ttttcgaccc tgcctgcgac tggggtgacc gagagtgcga cctagcgatg ttaccgctgc
     4441 atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg
     4501 atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaagattat
     4561 ttggcggtca gcatttggtt attgctcagc agtcattgga tagattatta gcagcatgat
     4621 atgggttgag gataatggcc gctccgtgcg gccttttgat taaataaacc caagcagaga
     4681 aaagaacaca taacctgcag caatacaaat aaccggcagg atatatagtg agaaaaactg
     4741 caggaaaata gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc
     4801 ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg
     4861 cgcctggcga ctcatccggg caccggaatc ctgcaacgcc agtccgacaa aaatcaggat
     4921 gaaaatcacc cagaacataa cgttcacacc accattaaaa ttaggggtcg gggagttata
     4981 ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagcaaa
     5041 gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag
     5101 caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt
     5161 caaaacagca atgcgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt
     5221 cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat
     5281 aggcgcttaa tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg
     5341 taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg
     5401 actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg ggggtagata
     5461 tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc
     5521 tttggtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     5581 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     5641 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     5701 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     5761 cggctgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     5821 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     5881 cttctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     5941 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     6001 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     6061 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     6121 gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     6181 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     6241 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     6301 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     6361 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     6421 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     6481 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     6541 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatcctg gttccccaaa
     6601 agggcaacca actggaacgt tttatctgca tggcattatt tgtctggtcg gtatggatgc
     6661 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaatgatc aataccttgc
     6721 agatccagtt tcatcggctt attaagcatc aggttgaata ctaaaaaggc aaaaatcacc
     6781 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     6841 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     6901 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6961 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     7021 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     7081 tacagtggtt taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc
     7141 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     7201 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     7261 tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     7321 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatcaga
     7381 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     7441 ccaatccgac gtcaattatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     7501 tgaaactgct gaaggatgat gcgccgaaag gggaacgcgt cttaaccgct atcgataccc
     7561 tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     7621 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     7681 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgctgtgcat ggcattctgc
     7741 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     7801 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     7861 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     7921 gtcagaacgg ctgcgccggt ttgtatccgg caatgctgcg gtgatggttg cgcctacggt
     7981 tggcattaac ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     8041 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     8101 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     8161 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     8221 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     8281 ccgttaattc ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta
     8341 aatgttccat cgcggcgatg catactgact catattt
//
LOCUS       DAJTSF010000145         8330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000145 DAJTSF010000000
VERSION     DAJTSF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.145"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..157
                     /locus_tag="OO335_003670"
     CDS             <1..157
                     /locus_tag="OO335_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003899.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(166..564)
                     /gene="vapC"
                     /locus_tag="OO335_003671"
     CDS             complement(166..564)
                     /gene="vapC"
                     /locus_tag="OO335_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR6003900.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(564..791)
                     /gene="vapB"
                     /locus_tag="OO335_003672"
     CDS             complement(564..791)
                     /gene="vapB"
                     /locus_tag="OO335_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR6003901.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            873..1244
                     /locus_tag="OO335_003673"
     CDS             873..1244
                     /locus_tag="OO335_003673"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR6003902.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1265..6126
                     /gene="traI"
                     /locus_tag="OO335_003674"
                     /pseudo
     CDS             1265..6126
                     /gene="traI"
                     /locus_tag="OO335_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6146..6892
                     /gene="traX"
                     /locus_tag="OO335_003675"
     CDS             6146..6892
                     /gene="traX"
                     /locus_tag="OO335_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR6003903.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6947..7507
                     /locus_tag="OO335_003676"
     CDS             6947..7507
                     /locus_tag="OO335_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6003904.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7638..7850
                     /locus_tag="OO335_003677"
     CDS             7638..7850
                     /locus_tag="OO335_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6003905.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8094..>8330
                     /locus_tag="OO335_003678"
     CDS             8094..>8330
                     /locus_tag="OO335_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6003906.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1912 a         2086 c         2671 g         1661 t
ORIGIN      
        1 cagtgaatcc ggtgaagtgg tggatatggc cgcttatgag gcatggcagc aggaaaatca
       61 tccggacacc tggcagcaga tgcagcgtcg tgaagaggtg aacatcaatg tgcaccggga
      121 acgcggggag gatgttgagc cgggagatga tttctgatga acaggtcagc tccagtcttc
      181 agttctcagg ccgcccacac gttcaaattc ccgggtgtta ttagtcacaa taatcagccc
      241 ccgactgcgg gcatgacctg cgatcatttg atcaaatggc ccgacagggc gtccctgacg
      301 ggcaagttct gctcttatct ggccggtgtg tgtggctgca gcagcgtcgt aatccagaac
      361 atcaatgcgg gaaacaaatc cctcgatcac agcgagatta cgttcaggca tctggctttt
      421 ttctgcacca tatatcagct ccatcagggt gaccgaactg atacacattt tcccctggtt
      481 caggttaaaa cgctccctga cgctggcggg tttgttcttt atcgtaaaaa tgcagatgtt
      541 ggtatcgagc ataaacttca gcatcagaat gactcccttt cttgcatgcc gggctgttcc
      601 ctgttatcca taaaatcggt gctgacgctg tgtccgtcga accattcgtc ccatgtctct
      661 ccggctggcg taatgattct ggttcgtccg acagcaatca cttcaacgcg ttttacgttt
      721 tccggcaatg caaccgcttt tggcagtctg accgcctggc tgcggttgct gagaaatacg
      781 gtggtttcca ttatttgcct ccttatgtgg atatgtcttg tgtatatcta ttgtgggatt
      841 gataccggga tatgtcaaag ggatatacgt ttatgatgag tatcgcgcag gtcagatcgg
      901 ccggaagtgc cggtaattat tataccgaca aggataatta ctatgtgctg ggcagcatgg
      961 gagaacgctg ggccggccag ggggctgaac aactggggct gcagggcagt gtcgataagg
     1021 atgtctttac ccgccttctg gagggcaggc tgccggacgg ggcggattta agccgcatgc
     1081 aggatggcag taacaggcat cgtcccggct acgacctgac cttctccgct cccaaaagta
     1141 tctccatgat ggccatgctc gggggggaca aacgtcttat tgaagcacat aaccaggccg
     1201 tggattttgc tgtccgtcag gtggaggcca gtgcctccac ctgacggacg gacagtcaga
     1261 aacggtgctg accggtaatc tggtgatggc actgtttaac cacgacacca gtcgcgatca
     1321 ggaaccacag ttacacacgc atgcggtggt gactaatgtt acgcagtata acggcgaatg
     1381 gaagacactg agcagtgaca aagtggggaa aacggggttc attgagaatg tgtacgctaa
     1441 tcagattgcc tttggcaggc tctaccggga aaaactgaaa gagcaggttg aggcgctggg
     1501 ctatgaaact gaagtggtcg gtaagcacgg tatgtgggaa atgccgggtg taccggtgga
     1561 ggccttttcc ggacgctcac agactatccg ggaggccgtc ggggaagacg cctcgctgaa
     1621 atcccgggat gtggcggcgc tggatacgcg taaatccaaa cagcacgtcg atcctgaagt
     1681 cagaatggcc gagtggatgc agacgctgaa ggaaaccggg ttcgacatcc gggcatatcg
     1741 tgacgcagcg gaacaacggg catatacccg cacgcagaca cccggacccg cttcacagga
     1801 cgggccggat gtgcagcagg cggtgacaca ggccattgcc ggattaagtg aacgcaaagt
     1861 gcagtttatg tatacggacc tgctggccag gacggtcggc atactgccgc cggaaaatgg
     1921 tgtgattgaa cgggcacgcg ccggtatcga tgaggccatc agccgtgagc agcttatccc
     1981 cctcgatcgt gagaaggggc tgttcacgtt cgggattcat atgctcgatg agctgtcagt
     2041 ccgggcactc agtcgtgaca tcatgaaaca gaaccgggtg accgtacatc cggagaaaag
     2101 tgtcccccgg acggccggtt acagcgatgc cgtcagtgtt ctggcacagg accgtccgtc
     2161 gctggccatt gtgtccgggc agggcggtgc agccgggcag cgtgagcggg tggctgaact
     2221 ggtcatgatg gcccgggagc aggggcggga ggtgcagatt atcgctgctg accgtcgctc
     2281 gcagatgaac ctgaagcagg atgaacggct gtccggtgaa ctgataaccg gacgtcgtca
     2341 gttgctggaa ggaatggcct tcacgccggg cagtaccgtt atcgtcgacc agggcgaaaa
     2401 actctccctg aaagagacgt taaccctgct ggatggtgcc gcacgtcata acgtacaggt
     2461 cctgataacc gacagcgggc agcgaaccgg tacaggcagt gcactgatgg ccatgaagga
     2521 tgccggggtg aacacatatc gctggcaggg gggagaacag cgaccggcca ccatcatcag
     2581 tgaaccggac cgtaatgtcg gctggccgga gattttgcgg ccagcgtgaa agccggagaa
     2641 gagagcgtgg cacaggtcag cggagtacgg gaacaggcca tactgacaca ggccattcgc
     2701 agtgagctga aaacacaggg cgtgctcgga cacccggagg taaccatgac cgccctgtca
     2761 ccggtctggc tggacagccg gagccgttat ctgcgggata tgtaccgccc gggaatggtg
     2821 atggagcagt ggaacccgga gacacgcagt catgaccgct atgtgacaga acgtgtgacg
     2881 gcgcagagtc acagcctgac cctgcggaat gcgcagggcg aaacgcaggt ggtgcgtatt
     2941 tcttccctgg acagcagctg gtcgctgttc cggccggaaa aaatgccggt ggcagacggt
     3001 gagcgtctga gggtgacagg gaaaattccc ggactccgcg tctccggcgg tgaccgcctg
     3061 caggtggcat ccgtcagtga agatgcgatg acggttgttg tgccgggacg ggctgaaccg
     3121 gccaccctgc ctgtgagcga ttcaccgttc actgcactga agctggagaa cggctgggtg
     3181 gaaacgcccg ggcattccgt cagcgacagt gcgacggttt ttgcctccgt cacacagatg
     3241 gcaatggaca atgccaccct gaatggtctg gcccgcagtg gtcgtgatgt ccggctgtat
     3301 tcctcactgg atgaaacccg tactgcggaa aaacttgccc gccatccctc ctttacggtg
     3361 gtttctgagc agataaaggc gcgtgccggt gagacattgc tggaaaccgc tatcagtctg
     3421 cagaaagccg ggcttcacac gccggcacag caggccattc atctggccct tcctgtgctg
     3481 gaaagtaaaa acctggcctt cagcatggtg gacctgctga cagaggcgaa gtcgtttgct
     3541 gcagaaggaa ccggttttgc tgacctggga ggggaaatca atgcgcagat aaaacggggt
     3601 gatttactgt atgtggatgt ggcaaaaggc tatggcacag gcctgctggt ttcccgtgcg
     3661 tcgtatgagg cagaaaagag cattcttcgc catattctcg aaggtaagga ggcggtcacg
     3721 ccgctgatgg agagagtacc tggcgaactc atggagaaac tgacatcagg acagcgcgcc
     3781 gccacccgta tgatactgga aacgtctgac cgtttcacgg tggtgcaggg ctatgccggt
     3841 gtgggtaaga ccacacagtt ccgggcggtg atgtcagccg tgaacatgct gccggagagt
     3901 gaacgtcccc gtgtcgtggg gctggggccc acacaccgtg cggtcgggga gatgcgcagc
     3961 gccggcgtgg atgcacagac actggcgtcc tttctgcatg acacgcagct gcagcagcgc
     4021 agcggagaaa ctccggattt cagcaacacg ctgttccttc tcgatgagag ctctatggtg
     4081 ggcaataccg acatggcacg ggcatacgcc ctgattgcgg ccggtggcgg tcgggctgtg
     4141 gccagcggtg acacggacca gctgcaggcc atcgcgcccg gtcagccttt ccgtctccag
     4201 cagacgcgca gtgctgccga tgtggtcatc atgaaggaga ttgtgcgtca gacgccggaa
     4261 ctgcgggagg cggtatacag cctgattaac cgggatgtgg aaagggcgct gtccgggctt
     4321 gagcgtgtga aaccgtctca ggtgccacgt ctggagggcg catgggcacc ggagcactcc
     4381 gtgacggagt tcagtcacag ccaggaagcg aaactggcag aagcgcagca gaaggcgatg
     4441 ctgaaaggcg aggcttttcc ggatgtcccc atgacactgt atgaagccat tgtccgcgac
     4501 tataccggca ggacaccgga agcacgggag cagacgctga ttgtcacgca cctgaatgag
     4561 gaccggcgcg tactgaacag catgattcat gatgcacggg aaaaggccgg agaactgggg
     4621 aaggtgcagg tcatggtgcc tgtcctgaac acagcgaata tacgcgacgg ggagctgcgt
     4681 cgtctctcca cctgggagaa taacccggat gcccttgccc tggtggataa tgtgtatcac
     4741 cggattgccg gtatcagtaa ggatgacggg ctgataaccc tgcaggatgc ggaaggtaac
     4801 acgcggctga tttcgccccg ggaggcggtg gctgaaggcg tcacgctgta caccccggac
     4861 accatccggg tggggaccgg tgacaggata cgcttcacga agagtgaccg ggagcgcggt
     4921 tatgtggcca acagcgtctg gacggtgaca gcggtttccg gtgacagtgt cacactttct
     4981 gacggtcagc agacccgtgt gattcgcccc ggtcaggagc gggcagagca acatattgac
     5041 ctggcctatg ccatcaccgc ccacggtgca cagggggcga gtgaaacctt tgccatcgcg
     5101 cttgaaggca cggaaggtaa ccggaaactg atggccggct ttgagtcagc ctacgtggcc
     5161 ctgtcgcgta tgaagcagca tgtgcaggtg tacaccgata accgtcaggg ctggacggat
     5221 gccatcaaca atgccgtaca gaaaggaact gcccacgatg tgtttgagcc gaaaccggac
     5281 cgggaggtca tgaatgcaga gcggctgttc agtacggcgc gggagctgcg ggacgtggcg
     5341 gcaggccgtg ccgttctccg tcaggcgggg ctggccgggg gagacagtcc tgcacggttt
     5401 attgctccgg gacgtaaata tccgcaaccg tatgtggcac tgccggcgtt tgaccgtaac
     5461 ggcaagtccg ccggtatctg gctgaacccg ctgaccacgg atgacggaaa cgggctgcgg
     5521 ggattcagcg gtgaaggtcg ggtaaaaggc agcggggatg cgcagttcgt ggccctgcag
     5581 ggcagccgta acggagaaag cctgctggct gataatatgc aggatggcgt ccggattgcc
     5641 cgtgataatc ctgacagtgg tgtggtggta agaatcgccg gtgaaggccg tccgtggaat
     5701 cccggtgcca tcaccggtgg ccgcgtgtgg ggggatatcc cggacaacag cgtccagccg
     5761 ggagccggaa atggtgaacc ggtcacggca gaggtactgg cacagcggca ggctgaagag
     5821 gccatccgcc gtgaaacgga acgtcgcgca gatgaaattg tccgtaaaat ggcagagaac
     5881 aaacctgacc tgccggacgg caaaacagag caggctgtca gggaaattgc cgggcaggag
     5941 cgtgaccggg ctgccataac tgaacgggaa gccgcgctgc cggagagtgt actgcgtgaa
     6001 ccacaacggg tgcgggaagc ggtccgggaa gttgcccggg aaaatttgct gcaggagcgt
     6061 ctgcagcaga tggagcggga tatggttcgt gacctgcaga aagagaaaac cccgggcgga
     6121 gactgataca ggaagacaaa cactgatgac aacggataac acgaacacga cacgtaacga
     6181 ttcactggct gcacggaccg atacctggtt gcagtcattt ctggtctggt cacccggaca
     6241 gcgggatatc atcaaaacgg tggcactggt gctgatggta ctggaccaca tcaatctgat
     6301 attccagctg aagcaggaat ggatgtttct ggccgggcga ggagcctttc cgctttttgc
     6361 cctggtgtgg gggctgaatc tgtcccgtca tgcgcatatc cggcaaccgg ccattaaccg
     6421 gctgtgggga tggggaatta ttgcccagtt cgcatattat ctggcaggct ttccttggta
     6481 tgaggggaat atcctgtttg cctttgcagt ggcggcacag gtgctgacgt ggtgtgaaac
     6541 gcgcagtggg tggcgtacag cagcggccat actcctgatg gccctgtggg ggcctttgtc
     6601 cggcaccagt tacggtattg ccgggttgct gatgctggca gtcagcaacc ggctgtaccg
     6661 ggcggaagac agagcggaac gtctggcgct ggtcgcctgc ctgctggctg ttattccggc
     6721 gcttaacctt gccaccagtg atgcagcggc ggtggccggt cttgtgatga cggtgctgac
     6781 cgttggtctg gtgttgtgtg ccggaaaatc attaccccgt ttctggcccg gagacttttt
     6841 cccgacgttc tatgcctgtc atctggctgt gctgggcgtt ctggcgctgt gacgggtgtg
     6901 gtatctttgg ccgcaagagg atgattcgtc agaggcagaa cacagcatga cagagcagaa
     6961 gcgaccggta ctgacactga agcggaagac agagggaaca gcgcctgtcc gcagccggaa
     7021 aaccatcatc aatgtcacca cgccaccaaa gtggaaggtg aaaaagcaga agctggccga
     7081 gaaagcggcc cgtgaagcag agctggcggc aaaaaaagcg caggccagac aggcgctgtc
     7141 catttatctg aacctgccca cgctggatga ggctgtgaat accctgaagc cctggtggcc
     7201 gggattattt gacggtgaca cgccccggct tctggcctgc ggtatccggg acgtgttact
     7261 ggaagacgtg gcgcagcgga atatcccgct ctcgcataaa aaactgcgca gggcgctgaa
     7321 ggccatcacc cgttcagaaa gctatctgtg tgccatgaaa gccggtgcct gccggtatga
     7381 cacggaaggg tatgtgacgg agcatatttc tcaggaggag gaagcgtatg cgggagcgcg
     7441 tctggcgaaa attcgtcgcc agaaccggat aaaggcagaa cttcaggccg tgcttgatga
     7501 gaaataaaaa aagcctcccc cagagagagg aggcagggaa ataaggctta aaaggaaata
     7561 acttccgctt ccgaaaggaa gccagtccac tgaattattt atcacaataa tactgacaac
     7621 taaaggggga caagaaaatg aatggattca gaaacagttc acggaacggc caggtctggc
     7681 gttaccagcg tgccggcggg cgggccgtta ttctggaagt cagtggacgc tggatggaag
     7741 cggcagaagc atggcgacgg gctgcctgtg ttgcgcctag gacagactgg cagcagtttg
     7801 cccggaaaag ggctgaacac tgccaccggc gctgccgggg aagggtgtaa cgaaaaaagc
     7861 agccttcatt actctaggag tcagaaggct gaaaggagtt tatgagattt gcttcttaat
     7921 atccggtcgg gcagtatgac cggacagttc tgagtagcac agagtctttt ccgcattaag
     7981 tcgatttttt atattttgtg attagtttca caaaataagg tgttattcaa tgtgtgctgc
     8041 actattcagg ggggctgaaa tacaggtggc atttatctga tatggagaat aacatgagga
     8101 aatatattcc actggcatta tttatctttt catggccggt attgtctgca gatattcatg
     8161 gacgggttgt tcgtgtactg gacggtgata ccatcgaggt catggactca ctgaaggcgg
     8221 tgcggatcag gctggtaaat attgatgcac cggaaaagaa acaggattat ggccggtggt
     8281 caacagacat gatgaaatca ctggtcgcag gaaaaacggt taccgtcaca
//
LOCUS       DAJTSF010000146         8279 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000146 DAJTSF010000000
VERSION     DAJTSF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.146"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            7..657
                     /locus_tag="OO335_003679"
     CDS             7..657
                     /locus_tag="OO335_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003907.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="OO335_003680"
     CDS             629..1063
                     /locus_tag="OO335_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6003908.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1836..2885)
                     /locus_tag="OO335_003681"
     CDS             complement(1836..2885)
                     /locus_tag="OO335_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6003909.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINTINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3336..4526
                     /locus_tag="OO335_003682"
     CDS             3336..4526
                     /locus_tag="OO335_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCR6003910.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4916..6376)
                     /gene="gtrII"
                     /locus_tag="OO335_003683"
     CDS             complement(4916..6376)
                     /gene="gtrII"
                     /locus_tag="OO335_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR6003911.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6373..7302)
                     /locus_tag="OO335_003684"
     CDS             complement(6373..7302)
                     /locus_tag="OO335_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6003912.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(7299..7661)
                     /locus_tag="OO335_003685"
     CDS             complement(7299..7661)
                     /locus_tag="OO335_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR6003913.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            complement(7923..>8279)
                     /locus_tag="OO335_003686"
     CDS             complement(7923..>8279)
                     /locus_tag="OO335_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6003914.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
BASE COUNT         2531 a         1597 c         1684 g         2467 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag
     1561 ccagtttatc agcgtcacca cctactgttt atgcttttat ttctttgatt aataagtata
     1621 tgatatttta attcatctgg tgtatttctt tctcatgtgt tggtcgactg aatatctgtt
     1681 cagtgaaggg gtaattttat gtttgcactt cgaacattcc cttcagttgc taggaaagaa
     1741 aaccgtaaca tttgcgcatt aattttaaca gtataatctg agcataaata tgcgctatat
     1801 atctataaaa aggcgcaatt aattgcgcct ttttatcatt ttatcaaaaa tggcccaccg
     1861 actgtcctcg atagccaatg gccaatttta tctgatacat atacacaaat acaggtatat
     1921 atgattgcgc agataggatt tggaatagtc acgctgaagc ctataattgc taaagccata
     1981 gggataaata atttggtgat atactccatt ccacatatgt taagagtatt tcttccagct
     2041 cttacaataa agtcatttcc acaaattatt tttgctatga aaatattaaa gattatcaag
     2101 cctaccgtgg tgataatagt attaatagct tgaatataac tacttgatat tatgtctgca
     2161 ttgaatattg agataatctt aaaaagtaaa ggtttctggt taagtagttg atacgcactc
     2221 attatagcgg taattgagaa gacaatgaaa ccaattttag attttccaaa aaacctgtca
     2281 cgtgttaatt ctaaaaacat acatctaccg agaggcaata accaccagta tgccatagcg
     2341 gagtctatgt tccaaaacca ttgaggatct agtaatgggt tatgattgag cactgtttgt
     2401 gacaacatga acgataacag tgatatgatt aaaatcacta tattgttctt gactatttct
     2461 ctaaagattg catcaattgc tattataaca aaaaggcagt ttataaacca tattgttcct
     2521 acgaattggt tatttctaac accatatatt atatcataaa tgtggttgta tatataatca
     2581 atcgtctcac ctgagttaat tgtgtttatt attaatatag aaaatgcaaa agtgaaatat
     2641 ggaatcatta atctgtaaaa ctttgacttg atataatcga aaacagataa gtcatttttt
     2701 ttgatagtaa aaaaaccagc agcgaaaaag aatagtggga cgtgataact aaaaacaaaa
     2761 ggatacaatt tgccagcccc caacccaagg tgacctaaat atatagcaaa aataccgaga
     2821 aattttaaag catcaaccca atcatgtctt atttttctgt tgctgtcgat tttgttatta
     2881 tacatttaaa gtaggctctc cagttttgtg tgatttttaa acactcattg caaatatgaa
     2941 tgcctgaaag tttatgatta ttaatcaacg agttcaatat taatttatta tttttcaaag
     3001 tcgcactata taggcattta cttccgcagt accaccgata gtatcgatat caaatcagcc
     3061 tgcggccgcg ataactttcg taacattctg ccagcggctt tgttgaataa atcaggttca
     3121 agataatcgt ctctgcatca ggttagagtt tcaggcaata catcctcttt ctggcatacc
     3181 tgccactctc gcaccatggt catagccttt acgatctgca tactcaccag accagagccc
     3241 gctccctttt aggctgagga atccccagaa aagaagacag gcatagagtt ttgtgatcta
     3301 ctgattgcgc taacaaattc aatgagatca cctctatgcc tgcccgtaaa gtatgccaga
     3361 cttttttccg taatgcttta gcttccacgc atcagtatcg acaaaatgcc attattgatt
     3421 ctgctgctgc tttagtcggt ggcgcatctc tttctcttac cagcattggc cggcatttac
     3481 ccggtcctgc tcgcgtgaaa gataaaataa aacgcgttga ctgtctgttg ggtaataaac
     3541 gacttcataa tgatatccct ctgatattca aaaatattac atccatgatg acaaataaac
     3601 tgtcgtggtg tgttatcgct gttgactgga gcggttaccc ttttcaggaa tatcatgtcc
     3661 ttcgtgccag cctcctctgc gacggtcgct ctatccccct gatgagtcag gtcttcccgt
     3721 caaaaaaaaa taacgaggcg gtggaaatcg cttttctgga tgcgctttcc ggggccattt
     3781 ctccccggac tcgtgttgtt attgttactg atgctggttt tcaaagtgca tggttccgtc
     3841 atatcaaatc acagggatgg gattttattg gtcgtatcag gggggtagtg aagttcaggc
     3901 tggatagtga caaagataag tggctggaca taaaaatgtg cagggggagt tcagaggcaa
     3961 aatacctggg gacgggaacc ctggcccgga aaaaacgctc gcagtgtgaa ggacattttt
     4021 atctttataa acattcacct aaaggcagaa aaagcaggcg tgccagaggg aggccgggct
     4081 taccaacaac ggaaaaagaa caaaaagccg caggcagaga accatggtta atattcagca
     4141 atacagctga gtttaatgcc aaaaaaatca tgaaactgta cagtcgaaga atgcagatag
     4201 agcaaaactt tcgggatgaa aaaagtgaac gctttggttt tggccttcgg gcgagcagaa
     4261 gtcggagggg agagcgtttt ctggtattaa gtctactggt tacactggcc agtatcgtac
     4321 tctggctact tggttatcat tttgaaaata aaggatttca tttaaagtat caggctaata
     4381 gtctcaaaaa acgaagagta ttgtcttttc tgacgctggc tgaaaacgtc ctgagattta
     4441 accctgaact tctacgacga gcacaacctg aaaagatatt gtgccggttg gccagcactt
     4501 accgaagtat ggtgctggct tactgatgaa aattttctgg ggatccctca gcctttcggg
     4561 gaggaatgag ggctctgata ttcttttgcc gtagttcatc gtgaaaccgt cggatgtcat
     4621 ttggcctgag tgacccatgc ctcccaaaag cagagtcgct agtgttatgt aattaggcgg
     4681 ttaaacttca attctcagtt ttaagtttgg gtaaacgctt gtaaatggta ttgctaattt
     4741 tttaattgac taatattaga aacggttagt gtgattgtat atagatattt gagtgtaagg
     4801 tatttaaagg ggcattcagc ccctccgtct atttgtgatt gcatccattg atgtgtgtat
     4861 tattttgagc ttgaaaaagg gaggcgctct ttaataataa ataaatctat caaagctaaa
     4921 tattaaatgg aagccaccct tttttctctg atagttttat tatcaccaaa ccatttattt
     4981 cagctatcga atctggattc ggccaggaag gtaaactctt cacaattgga taaatcttct
     5041 ctatttgttc tttatttgct ggtttgcaat tacagattcc atagtaattc ataaatgcaa
     5101 ccatcctaaa atagttgcca ccatcccacc aaaaaaaaga ttttccaaaa gtgtctgcgc
     5161 tcatggagag catatttcct gagtcgtttg aaccatatat atatatatat tttccgttaa
     5221 gggaaatgcc ttttgtttga gatgtgtatg atatttcttt agctaaagag atatctttct
     5281 gtcttgcgag agtatcattc aaaaatagat ttttggatgt tgaaacgcca ttaaatataa
     5341 taaataaaaa aaacatgcag tttaggtatt ttatagagcg aaaattgctt aagcaagaaa
     5401 acaaaattac tgctactata ggcattaaaa caaacaggcg aggtggcgcc cctgagccaa
     5461 caacaacgat aaatataacc ggaagtatat aaatgaaaat gattgatgaa atcaaataaa
     5521 ttgatcttgt ttttaattta tatgttaggg taaacatcag aattaataaa ggaatcagta
     5581 accacttgta taggtttaaa ccagtgggag gattgttata gaagttatat atattatcta
     5641 tggctgattt aacaagccat ttaatatcgc ttgcattttg tatataattt gagatgtact
     5701 cgttcgattc aagcgaaaaa taatgcttga tagctttggt taatagcagg taaattaagg
     5761 tagataaagc tatgagggat agagataaac atgaactata aaaagattca cgaatatttt
     5821 tctcatttcg tattatcgaa tttatctgca acccaatgac gaatgcaata atatatgtta
     5881 cgaatgtttg gtagattgcc attgaaagaa tggacagtac tataccagaa aatatcacaa
     5941 tcctattttt ttgcgagtga aaaataattg ctgatatcgc tgccagtaga aaagcaattc
     6001 ccacagtatc agcttggtta agaaactcta attgatagga gatctgaggg aaggtaataa
     6061 atactaacat acctatcaat aattcataag attctagctt tagcgatctg cagattataa
     6121 atgctgaaat gatgataaaa gataaggcta ttaatggtgt tatataaagt gaaaaaggct
     6181 cagggagaaa gtaatgtcgc aaaaaagtgt gaaaccaccg cccaagcgtg attgtttgat
     6241 aaaaattatt tgtaaactcg ccatcaatat taagtgttcc atcgtacaac cacccccaat
     6301 aacaataaat tgatattgca aaacatgata tgaaggcaag gagatttgca tttttaaata
     6361 aattaatttt aatcatgggt tacctcgatg agattttttt atgatgtatt tgggtctatt
     6421 ttttacttca atatagattc tacctatata ttctccgaga acaccaatcc cgatcagttg
     6481 cactccaccc aagaaaagta ttgatacaag cagggaggga tacccgcgta ctgggttacc
     6541 aaagacaagg gtgtctataa tcatccatgc accatataaa aatgaaatgc ttgcaacaaa
     6601 caagcctata taagtccata cgcgaagagg aaaggttgaa aaacttgtga taccttccag
     6661 tgccagattc cataatttcc agccattaaa ttttgagatg ccagcaacac gctctgcgcg
     6721 tacatattca acgacatccg tcggaccacc cacccagctc agtatgcctt tcatgaaaag
     6781 attgcgctca ggcaacagtt taatgttctc cacaacctca cgagacatga gtcgaaaatc
     6841 tccgacattt tcctcgatct ttggggtgct tattttgttg tgtaatttat agaaccactc
     6901 agctgtctta cgtttcagtc gtccatcagt tgagcggtct gaacgtttag caagcaccat
     6961 gtcagcacct gcctgccact tttcaataag atgagggata acctcaattg ggtcttgcag
     7021 gtcgacatca ataggaatta cagcatcgcc gcttgcatgg tctaaccctg caaataaggc
     7081 tggttcttta ccaaagttgc gtgtaaatga cagcggaaca actagcgggt ctgaaacagc
     7141 cagcgcgtta ataattgact ctgtggcatc tttacttccg tcatttatga atacaatttc
     7201 tacttcatat ggcttcaact cttggaattc acgtaccgtt ttatagaaaa caggtatcgc
     7261 ttcttcttca ttgaagacag gaacgacaag agatattttc atttcgcatc cctaaagaca
     7321 atgaactttg aatagacgaa accgcacacc agactgatgg cggagaaggt gataagagtg
     7381 actattggag gaagtgagca tttatcagct gcccaaccaa caatcacact caatattccc
     7441 ataaatccca cgtataacat gtagcgcatc gctgtagtca atgctttgaa tgtgaatctt
     7501 gcattcgcga agaagctaaa gctcacagcc actacgaaac ctgtgaagtt tgccagagcc
     7561 tgactggtat gtgcggcata gatacataca ccaaaaacca cccagtgtat aagtgtgttc
     7621 agcacaccta tagatgtgta ctttacaaat aactttaaca tttatttaat caatgagctc
     7681 tgaaaggcat gaagtctatc atccaagtct caattgatcg atacttgcta tgtctgatga
     7741 gacaaaactg agacacataa ggcctcacaa tggcttgcaa ggctttacat gttttgatgt
     7801 ggtgggacgt gtgagcgcag tgttgatggg ataatccttt gaattacaag cggattctta
     7861 taattcgtaa tgcgaaggtc gtaggttcga ctcctattat cggcaccatt taaatcaata
     7921 agttacctcg catttaagta aactacgttc tcctcttgtg ccgtatttgt gccattgcga
     7981 cttataatcg catcgatttt gctcgcgtgc tcggtaagat gcccggctga aaggtgggcg
     8041 tatctttgaa ccatttcgag agtttcccat cctcccatct ctttaagcgc aagaagagag
     8101 acaccggact gaaccagcca gcttgcccag gtatgcctca ggtcatggaa gcggaagttg
     8161 ctaatgcctg cccgctttaa cgctcccttc catgccttgt tgctgtcggt tctcatcttc
     8221 cttaccgctg ctgtttttgt tccgtcgctt cggtaggcag gtttggtgtg gacaaacac
//
LOCUS       DAJTSF010000147         8223 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000147 DAJTSF010000000
VERSION     DAJTSF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8223
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.147"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..304
                     /locus_tag="OO335_003687"
     CDS             <1..304
                     /locus_tag="OO335_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR6003915.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            276..2186
                     /locus_tag="OO335_003688"
     CDS             276..2186
                     /locus_tag="OO335_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6003916.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2187..2390
                     /locus_tag="OO335_003689"
     CDS             2187..2390
                     /locus_tag="OO335_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6003917.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2387..3980
                     /locus_tag="OO335_003690"
                     /pseudo
     CDS             2387..3980
                     /locus_tag="OO335_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3970..5475
                     /locus_tag="OO335_003691"
     CDS             3970..5475
                     /locus_tag="OO335_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR6003918.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5512..5859
                     /locus_tag="OO335_003692"
     CDS             5512..5859
                     /locus_tag="OO335_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6003919.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5917..6945
                     /locus_tag="OO335_003693"
     CDS             5917..6945
                     /locus_tag="OO335_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6003920.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6997..7383
                     /locus_tag="OO335_003694"
     CDS             6997..7383
                     /locus_tag="OO335_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6003921.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7395..7772
                     /locus_tag="OO335_003695"
     CDS             7395..7772
                     /locus_tag="OO335_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6003922.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7759..>8223
                     /locus_tag="OO335_003696"
     CDS             7759..>8223
                     /locus_tag="OO335_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6003923.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1905 a         1932 c         2541 g         1845 t
ORIGIN      
        1 actcattgcc agatgggaac tgaccgcaga acaggctgtc gcacagcagt taaaaaatca
       61 ggtgtcaaaa ggaaatctta tcgataccgg attttgtatt tttgccctta gtaagctggc
      121 gatggcttta tccagtacgc ttgactccat tcctttgtcc atgcaacgac agttcccgga
      181 tttaacgccg cgtcatattg atcatctgaa aatccttatt gctaagggag cgaatcagtg
      241 cgcgcgtgcg ggggataaat taccggattt actcgatgaa tatatcagaa caacaactga
      301 gtaatatgat ggtggcagtc actatcgcac tgcagccact ggtcagggta ttgccggtga
      361 cggctgtaga atgggccgat caaaattatt acctgcctaa agaatcgtca tacggtgagg
      421 gggaatggaa aacgctgcag tttcaggtcg ccatcatgaa ctgcatgggc aatgacgaga
      481 ttcgtacagt taacctgatt aaatctgccc gtgttggtta tacaaaaatg gtgcttggtg
      541 tgatcgggta ttttattgag cacaaatccc gcaacactct tctttttcag ccgacggact
      601 cagctgctga agattttatg aagtctcacg tggaagcaac aattcgtgat gttccttgcc
      661 tgaaaaaact ttctccctgg ctgggccgga aacaccggga taatacgctc acactgaagc
      721 gtttttcctc tggtgtgggg ttctggtgtt tgggtggtgc tgctgccaaa aactaccgtg
      781 aaaaatctgt cgatgtggtc tgctatgacg aactctcctc gtttgaaccg gatgtggaaa
      841 aagaaggttc gccaacactg cttggcgata aacgtattga aggttcggta tggccaaaat
      901 ccatacgcgg ttcaacgcca aaaattaaag gctcctgtca gattgaaaaa gctgcgaatg
      961 aatctgcgca tttcatgcgg ttttatgtcc cttgccctca ttgcggggag gagcagtatc
     1021 tgaagtttgg cgatgatgcg acgtcgtttg gcctgaaatg ggagaagggt aaaccggaaa
     1081 cggtgtatta cctgtgtgaa cataatggct gcgtgatccg tcagtctgaa cttgatcaga
     1141 ccggggggcg ctggatttgt gacaataccg ggatgtggac gtgtgacggt ctgacgtttt
     1201 tcagtgcttc gggtaatgaa attccgccgc cgcgctccat cactttccat atatggacgg
     1261 cgtacagtcc gttcaccacc agggtacaga tagtctgtga ctggctggat gcactgaaag
     1321 atcccaacgg cgtgaaaact tttgtgaaca ccacgctggg cgagacctgg gaagaggccg
     1381 tgggcgaaaa actcgatcac caggtactga tggataaggt tgtgccttac acggcaacgg
     1441 tgcctgtccg ggtggtttat ctgacggcgg gcattgactc gcagcgaaac cgttttgaga
     1501 tgtatgtctg ggggtgggct ccgggagagg aagcctttct ggtggataaa atcatcatca
     1561 tgggacgtcc tgatgaggaa gagacgctgt tacgtgtgga tgcggcgatc aataaaaaat
     1621 accgccatgc agacggtacc gaaatgacca tttcccgtgt ctgctgggat atcgggggga
     1681 tcgatggcga aatcgtgtat cagaggtcaa aaaaacacgg tattttccgg gtactgccgg
     1741 taaaaggtgc ctctgtctat ggcaagccgg tgatcaccat gccgaaaacc cgcaatcagc
     1801 ggggtgtgta tctgtgtgag gtggggacgg ataccgcaaa agaaattctc tatgcccgaa
     1861 tgaaggccga tcccacccct gtgaatgagg ccacatcgta tgccatccgc tttcctgata
     1921 atccggagat tttttcgcag acagaggcac aacaactggt ggcggaggaa ctggtggaaa
     1981 agtgggaaaa aggaaagatg cgtctgttgt gggataacaa aaagcggcgt aacgaagcgc
     2041 tggactgcct ggtgtatgcc tatgcggcat tacgtgtgtc cgtgcaacgc tggcaacttg
     2101 atctggctgt gcagttgcat cccgcaagga tgcaactgca caaccgaccc ttgaagaact
     2161 ggcagcgaag ctgtccggag gagtgaatgg ttacagtcgc tgaactgcag gcgctgcgtc
     2221 aggcgcgcct tgatttatta accggtaaac gggttgtgtc ggtacagaaa gacggtcgca
     2281 ggatcgaata tacggcagcg tccctgaatg agcttaaccg tgcgatcaac gatgcggagt
     2341 cggttctggg tacgacccga cgccgccgac cgctgggagt gaggttatga aacgaacgcc
     2401 tgtcctgatt gatgtgaacg gcgttccgct tcgtgagagt ctcagctaca acggaggcgg
     2461 cgcaggattt ggcgggcaaa tggcagagtg gttaccaccg gcgcagagtg ccgatgcggc
     2521 cctgttgcct gcattgcgtc tggggaatgc ccgggcagat gatctggtgc gcaataacgg
     2581 tatcgcggct aatgcggtgg ccctgcataa ggatcatatc gtcgggcaca tgtttcttat
     2641 cagctaccgt ctgaactggc gttggctggg gatgcgggag accacggcaa aaagttttgt
     2701 cggggaggtg gaagcagcct ggtcggaata cgcagaagga atgtttggcg agatcgacgt
     2761 ggaagggaaa cgcacgttta cggaatttat ccgtgaaggg gtgggcgtgc atgcctttaa
     2821 cggcgaaatc tttgtgcagc cggtctggga tacggaaacc acgcagttat tccgtacgcg
     2881 ttttaaagcc gtgagtccga aacgggtgga cacgccagga cacggtatgg ggaaccactt
     2941 tctgcgggcc ggggttgagg tcgatcggta tgggcgggcg attgcgtacc atatctgtga
     3001 ggatgatttt ccgttctctg gtgccggacg ctgggagcgg atcccgcgtg agcttcccac
     3061 cgggcgtccg gccatgctgc atatttttga gccggtggag gacgggcaga cccgtggggc
     3121 taatcagttt tacagcgtaa tggagcggat gaagatgctg gactccctgc aggccacgca
     3181 gcttcagtcg gccattgtga aggcgatgta tgcagccaca atcgaaagtg aacttgatac
     3241 cgaaaaggca tttgaatata tcgcgggggc accgcaaggt cagcaggata atccgcttat
     3301 taatattctg gagaagttca cccgctggta tgacacgaat aacgtgacgc tgggcggtgt
     3361 taaaattccg cacctttttt cctggcgatg cgctgaacct gcagacagcg caggattcag
     3421 acaatggatt ttcggcgctt gaacaggcgc tgctgcggta tattgcagcc ggtcttggcg
     3481 tttcctacga acagttgtcc cgtgattact cgaaggtcag ttactcaagt gcccgcgcct
     3541 ccgccaatga gtcgtggcgc tattttatgg ggcggcgaaa atttattgcg gcccggctgg
     3601 ccacgcagat gttttcctgc tggctggaag aggcgcttct tcgggggatt attcgtccgc
     3661 cacgggcgcg ttttgatttt taccaggcgc gttcagcctg gtcacgggca gagtggatta
     3721 gctccggaag aatggccatt gacgggctca aggaggtcca ggaatcggtg atgcgcattg
     3781 aggccggact gagcacgtat gagaaagaac tggcactgat gggcgaagat tatcaggaca
     3841 ttttccgcca gcaggtcagg gaatccgcag agcgcgaaaa agccggactc tcacgtccgg
     3901 tgtggatagc ccaggcgtat cagcagcaga tagcggagag tcgcaggccg gaagaggaga
     3961 caacatcacg tgagacgtaa tctttcacat attatcgctg cggcattcaa tgagccgctg
     4021 cttctggagc ccgcctatgc gcgggttttc ttttgcgcgc tcgggcgtga gatgggcgca
     4081 gcaagccttt cggtaccgca acagcaggta cagcttgacg ctcccgggat gctggctgaa
     4141 acggacgagt acatggccgg aggcaaacga cctgcccgtg tttaccgggt ggtgaacggt
     4201 attgctgtac tgccggtgac cggcacgctg gtacaccggc tgggtggcat gcggccattt
     4261 tccggaatga ctggctatga cggcattgtc gcctgtcttc agcaggcaat ggcggacact
     4321 caggtacggg gcgtactgct ggacattgac agttctggcg ggcaggccac cggcgcgttt
     4381 gactgcgctg acatgattta ccgtctccgg cagctgaagc ctgtctgggc actgtgcaat
     4441 gacacggcct gttcagccgc catgttactg gcatcggcct gctcccgacg gctggttacc
     4501 cagacatccc gtatcggctc cattggggtg atgatgggcc atgtcagcta tgccggtcat
     4561 ctggcacagg ccggtgtgga tatcacgctg atttacgccg gatcgcacaa ggtggatggc
     4621 aatcagtttg aatccctgcc agcaaaggtt cgccaggaca tgcagcagcg cattgatgcg
     4681 gcgcgccgga tgtttgctga aaaagtggcg atgtataccg gtttatctgt ggatgcggtt
     4741 atgggaacag aggccgccgt ttttgaaggt cagtccggta ttgaggccga actggcggat
     4801 gaattaatca atgcgtcgga cgccatcagc gtgatggctg cgacgctgaa cacacacgat
     4861 acaggaggca ctatgccgca attaactgca acggaggctg ccgcgcagga gaaccagcga
     4921 gtgatgggga tcctgacgtg tcaggaagcg aaaggacgtg aacaactggc cacgatgctg
     4981 gcagggcaac agggtatgag cgttgagcag gcccgggcga ttctggccgt ggcggcaccg
     5041 caacaatctg gtgtatctgc gcagagtgaa gccgatcgca ttatggcatg tgaagaggcg
     5101 aaaggacgtg aacaactggc ggcaacgctg gcggcgatgc cggagatgac ggtggaaaaa
     5161 gcccgtccga ttctggctgc ttcaccgcag gcgaatgccg gaccctcact tcgtgatcag
     5221 attatggccc tggatgaggc aaaaggggca gaggcgcagg ctgaaaaact ggcggcctgt
     5281 ccggggatga ccgtggagaa tgcccgggct gtactggcgg cagcctcagg cagggcagaa
     5341 ccggtttctg catccacaac cgccatgttt gaacatttca tggcgaacca ttcaccggca
     5401 gcggtacagg gtggcgtgcc acagacgtca gcagacggtg atgcggacgt gaaaatgctc
     5461 atggccatgc catgaagtca gtgctgacca tcaatatgag gtttttacaa tatggtaacg
     5521 aaaaccatca ctgaacagcg tgcggaagta cgtatttttg ccggtaatga tccggcccat
     5581 accgccacag gctgcagcgg gatttcctcg gcaacaccgg ctctgacgcc cctgatgctg
     5641 gatgaggata ccgggaaact ggtggtctgg gacggacaga aggcaggcca tgtggttggc
     5701 atactggtac tgccgcttga aggtacagag acggtgctga cgtattacaa gtcggggacc
     5761 tttgcgacgg aggccatcca ctggcctgag agagtggata cacacaaaaa ggcgaatgcc
     5821 tttgccggca gtgccctgag tcacgcagcg ctgccgtaac attttatcag gtcactgcgg
     5881 tagcctgact gatttctgaa tgaaaggaac tgatttatgg gattgtttac gacccgccag
     5941 ttactcggtt ataccgaaca aaaagttaaa tttcgtgcgc tgtttctgga gctgtttttc
     6001 cgccgtacgg tgaatttcca taccgaagag gtaatgctgg acaaaattac cggaaaaacg
     6061 ccggtggcgg cctatgtctc cccggttgtt gaaggaaagg tgttgcgtca ccgtggcggt
     6121 gaaactcgtg tactgcgccc gggctacgtt aagccgaaac acgaatttaa ttaccagcag
     6181 gcggtggagc gtcttcccgg tgaagatccg gcccagctga acggcccggc ttaccgtcgc
     6241 ctgcgtatca tcaccgataa cctcaaacag gaagagcacg ccattgtcca ggtggaagag
     6301 atgcaggcgg tgaatgccgt gctgtatggc aaatacacca tggaaggaga gcagtttgaa
     6361 actgttgagg tcgattttgg ccggtcggtg ggaaataaca ttacgcaggc aggagggacg
     6421 gagtggagcg ctcaggaccg cgataccttt gatccgacgc atgatctgga tgcgtactgt
     6481 gattttgctt ccggcaccat caatattgcc attatggatg gtaaggtctg gcgtctgctg
     6541 aatggcttta agctgttccg cgaaaaactg gatacccgtc gtggctcaaa ttcacagctg
     6601 gaaacggcag tgaaagacct gggggcggtg gtgtccttca aagggtatta tggtgatctg
     6661 gcaatcgtgg tggcaaaaac gtcctatgtg gcagaggacg gtaccgaaaa acgttatctg
     6721 ccggacggca cactggtact gggaaatacg gccgcagagg gtattcgttg ctacggtgcc
     6781 attcaggatg cgcaggcatt gtctgaaggc gtggtggcgt cttcccgtta tccgaaacac
     6841 tggctgaccg tgggtgatcc ggcgcgtgaa ttcaccatga cgcagtccgc gccgctgatg
     6901 gtgctgccgg atccggatga gtttgtggtg gtgcaggtga aataatccgt gagcgggagc
     6961 taaatgctcc cgtttttcat tcaatcagga gcgggtatgg ctacgaaaga agaaaacctg
     7021 aaacgtcttt gtgagctggc tgagaggctg gggcgtgaac cggatgtctc cggtagtgcg
     7081 gcagatattg cgcagcgagt ggcggaactg gaggaagagc ttggagatgc gggggaggtc
     7141 gcagagccgg atacgtactc accatcgaaa gatgatccga ccgcgcataa aggcgagtca
     7201 gttcctgaac agccaggcat tgttaccgga gacgaaacgg ggcaggttac ggtggtggca
     7261 ctggcgacac tgcatacaaa atgtaatggt tcagtgatat ttgtttcacc gggaacgtcg
     7321 tttcgtgttt ctgctgaggt ggctgccagc atggcggcag gtggtctggc aaaaaggcag
     7381 taacaggaga tgtcgtggac ggtttcgata atctgtttga tgctgcgctt gccggtgtca
     7441 atgaagtcat tctccgtgat atggggatca gcgccgtcat tacgtccgga gaacttgagg
     7501 ggacacacct gacaggggtt tttgatgatc ccgaaagtat ttcttttgtt gccgggggga
     7561 tccggcttga ggattcctca ccgtgcctgt ttgtgaaaac ggcagatata tcgcagttac
     7621 gccgtcagga tacgttaact atcggggatg attctttctt tgtggatcgt attactccgg
     7681 atgacggggg atgctgttat atccggttaa ggcggggcag tcctgcccca cagaataatg
     7741 ccagaatgag gcgatatgat gaagggactt gagaatgcca tccgcaatct gaacagcctc
     7801 gatacccgca tggtgccaca gaccagcgca tgggcggtaa accgtgtggc ggcaaagatt
     7861 gtttctgtcg ccacacggca ggttgcgcag aataccgttg ccggggataa ccaggtgaaa
     7921 ggtattcccc tgaaaacggt cagggagcgt gtcaggctgc ttaaagccag cccctcagga
     7981 aaaatgtatg cccgcatgcg tgttaaccgg ggtaacctgc ccgccattaa gctgggtacc
     8041 gcacaggtca gactggcccg ctcccggcat ggcagcaact cacgtcatcg tggcagcgta
     8101 ctgaaagtgg ggaaatacct tttccgggat gcgtttattc agcaactggc gaatggtcgc
     8161 tggcatgtga tgcggcgtat tgacggcaaa aatcgttacc ccattgatgt ggtgaaaatc
     8221 ccg
//
LOCUS       DAJTSF010000148         8080 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000148 DAJTSF010000000
VERSION     DAJTSF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.148"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..248)
                     /locus_tag="OO335_003697"
     CDS             complement(<1..248)
                     /locus_tag="OO335_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6003924.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
     gene            complement(335..696)
                     /locus_tag="OO335_003698"
                     /pseudo
     CDS             complement(335..696)
                     /locus_tag="OO335_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1035..1589)
                     /locus_tag="OO335_003699"
                     /pseudo
     CDS             complement(1035..1589)
                     /locus_tag="OO335_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1732..1854
                     /locus_tag="OO335_003700"
                     /pseudo
     CDS             1732..1854
                     /locus_tag="OO335_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="OO335_003701"
     CDS             complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="OO335_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR6003925.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2964..3278)
                     /locus_tag="OO335_003702"
     CDS             complement(2964..3278)
                     /locus_tag="OO335_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003926.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3356..3466)
                     /locus_tag="OO335_003703"
                     /pseudo
     CDS             complement(3356..3466)
                     /locus_tag="OO335_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="OO335_003704"
     CDS             complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="OO335_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR6003927.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4839..4976)
                     /locus_tag="OO335_003705"
                     /pseudo
     CDS             complement(4839..4976)
                     /locus_tag="OO335_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5058..5356)
                     /locus_tag="OO335_003706"
                     /pseudo
     CDS             complement(5058..5356)
                     /locus_tag="OO335_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5945..6104)
                     /locus_tag="OO335_003707"
                     /pseudo
     CDS             complement(5945..6104)
                     /locus_tag="OO335_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6108..7337)
                     /locus_tag="OO335_003708"
                     /pseudo
     CDS             complement(6108..7337)
                     /locus_tag="OO335_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7357..7704)
                     /gene="tnpB"
                     /locus_tag="OO335_003709"
     CDS             complement(7357..7704)
                     /gene="tnpB"
                     /locus_tag="OO335_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6003928.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7704..>8080)
                     /locus_tag="OO335_003710"
     CDS             complement(7704..>8080)
                     /locus_tag="OO335_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6003929.1"
                     /translation="RRPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQ
                     YRKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLR
                     LKGKLTPALLQILIREIKGSSH"
BASE COUNT         2068 a         1884 c         1776 g         2352 t
ORIGIN      
        1 cgagcgatta ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg ttgacgaata
       61 taatcccggt attcatccag gagtgaagca acagcaggtc gcggcgtata ttttggcggc
      121 tcagattttg cctgcaaata acgtttaacg gtattgcggg agagccccag ttctctggca
      181 atcgcccggc tactcattcc ctgcttgtgc aggattttaa tttccataac tgtctcaaaa
      241 gtgaccataa gctctcctga atcaggagag cagattaccc cctggatctg atttcaggcg
      301 ttgggtgtgg atcactattg caccgttcgt gacagtgttg ttgcgccttg ctgccaacga
      361 agctccactg cccatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag
      421 atgttatccg ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt
      481 cagcgtacgg ccagtatcgc gaactccggc accgttgaaa gccatttcag tgatttggtt
      541 tttaactccc ggtttacggg cctcgtaaca gtaagtgagc tgaaacaccc ggtgacagtc
      601 acggcaacgg aatcgatcat gtccttttgg gttctgcccg tgacggtaaa cctgaacgga
      661 ctggcaacgg gggcaatgaa agttaactgt gaccatgaaa gaacctaaaa gcgggtatta
      721 taacccctaa ttcaactaat tggaggcatt gtcattttta atctatggat acacttaaaa
      781 atcctctcac cttacgttaa aaaccttctc atgtttagag ttttttatta tgtttatcat
      841 aatgatatga cgatctgagt ttttaaaatc aagaagaaaa gataagtttt ttaaatgcac
      901 agtgcctgtg gataactttg cgtaaatccg gaggacagat cactctgaag caagacaaca
      961 gcatctgaaa acaatatgct cacgtttcaa ccacagggcg taagtgatcc tacccacgta
     1021 atatggacac aggcctaagc gaggttctgg ttttcaaatt gttccggact gaggccgcca
     1081 caccaactgt gccgtcgcca gcgattgtaa tcacattcga tataattaaa cactgttgcc
     1141 ctcattattt cccggctgat aaagtgttct ccgtggagac attccacttt cagcgaatga
     1201 aagaagcttt ccacgcaggc attatcgtag cagcagccct ttgcgctcat actgccacgc
     1261 agattatgcc acttcagcag cgcctgataa tctgctgaac agtactgact tcctcggtcc
     1321 gagtgaacga taacgttccg gggtctctta cgccgccaca gcgccatctg cagggcatcg
     1381 caggccagtt gcgccgtcag gcgtggtgac atggaccagc caataacggc acgtgaccac
     1441 aggtcaatga ccacagccag atacagccag ccttcatctg tacgtaagta cgtgatgtct
     1501 cccgcccact tctggttcgg accactggcg taaaaatcct gctccagcag attttctgac
     1561 acaggcaggc catgtgcgcg gtaactgacc gtattgctgg gtaagatcaa tttgctcact
     1621 ggtatttact acgtgcatcg aatgagtgat caagagccgt atgacgcgag agtgtcacgt
     1681 acggttccgt aagcagccgg agagtgaaac tctctccggc tgactcgact cgcaggttcc
     1741 gacagcggtg acgaacatgc agcggtgttg ttctcgctga tcggcacatg ccgtctgaac
     1801 aatgtggagc cagaaaaatg gctgcgttac gttattgaac atatccagga ctgaccggca
     1861 aaccgggtac gcgatctgtt gccttggaaa gttgatctga cttctcagta aatatcaata
     1921 tgattctgac gagccgctta cctttgagca gagaaagggg agagggatcc cacatccatg
     1981 agctaggaaa atacaaaaac tggctataca acaattcaat atattaggac ttacttgcga
     2041 ggagaaatat agcaccgctc aggcaattcc tgggtattta cttttcttag tttctttact
     2101 cgggatgttc tgtcatcaga aaggcgattc taaatttgta atatagtcac tcatattccc
     2161 acgtaatttc tttagatcaa tatattgcac atttttctca aaaaactgat cggaaataag
     2221 atttgcaaca ttgcttgttg tatcattttt aacgcttcta ttcatccctt cacaaagttc
     2281 gtccattttc tttttgaaat aactgcttcc tgatacaaga tgatttatgg aactttgtgc
     2341 agcattgatg tctagagaaa tgcgataatc tttctcaacc tgtttaaata tctgtttacg
     2401 gagattatcg ccgatttttc ggctgtgttc tgcacaaaac ttatcaatac attggtttac
     2461 cacccgggat acgtagactt ttttttcatc atttttccaa tctgtaatat tttcaccgat
     2521 ttgttctgaa acagagctca aaatactagt atttattttg gttgatgata tttttactat
     2581 ccttttcccc attgtacagc ggataatctc atccaccttt cctgaaaaat atcttttgtg
     2641 agataaactg attccaaaat tattaaaaga tgttccaagc attataatca ccttgtcatg
     2701 taaaatagta acattgtcat tattttagct gattcaaaca attctgtaaa tgctttgagt
     2761 tctttactcc cttggctcaa tgatggaccg tctggaatcc cagagactaa actctctgag
     2821 aaagagagta tgaaaaaact tggggcagtc cctaaatgaa gggaccaaca gcggtaaaat
     2881 aaccggcatc attcataatg atgccctgtt agttgtagtt atgaagattt acgtcattaa
     2941 aattgctgtt cgtagtgtca gtcctatggt ctggcatcag ctgaggatcg tagccgatac
     3001 atcactggct tcacgacatt cgtgttgagg ctattcatga aaactccacg ctgaaaacgc
     3061 cgttttgtat aaacggtcac ggtatgcccg gagccactgt tgctgataaa ttcgataaaa
     3121 ccttggcgtt tcttgaagct atcgttaatg cggataatga aacaacgatt ggtgagattc
     3181 gctcttttgc cgacgccctg gacgctgtcc ggttcaatcg caacaaaatc aaccggcagc
     3241 tgagcaaacc caaccttgcc tctctggcgc tggagcatga agtgatttgg ctgggacgta
     3301 gccgctgaca gcttctataa aagtatgatc ctacccacgt aatatgaaca caggcttaag
     3361 tgaggttctg gttttcaaat tgttcaggac tgagacctcc acaccaactg tgccgtcgtc
     3421 agcgattgta atcgcacttg atataattaa acactgtata tttcatttgt ggagtctcca
     3481 gtctgagagg gctttatgca ttccgtattg ggcactatga tgccagaaat ctgcaaggct
     3541 ccacaccttc ctgtatttcc cttcctctat cacagtgcac tttacttaat agggtgggat
     3601 gtgttcatta catttattgt gttttttatg attgtttttg cgtccagtgt cgttcgtcat
     3661 gggaaactgg ttctatgggt agtatcgctc agtctagtta actgtttgtt tctttttaga
     3721 aattaagcag atcacacaat tcggggagag atccttctaa gtgcaattct ttgcagctat
     3781 ttagcacact taaaacaact cttgtatttt gtcagaaata atattaaaca tgtcttgttc
     3841 attattgtta agtctgtctg ctatttctgt aagttttatc ccactatgat ggcatatatt
     3901 tatatatgcc atcatagcct cgatattccc tttccctgcg gccagaaata atcctggtaa
     3961 cccctcatta tctcttacat ccaatagttt aatcgtttct tttggtgata aatcaaaagt
     4021 tttaataaaa tctccataag cttgaatcgc agcggaatga ccttcttgaa gagctatgaa
     4081 caaagcagat tccccagcat tatttcgtgc tgcaagaact tctagttttt cctctttggg
     4141 tatttcggct gccaacagcg attcaccgta tttacgcgct gcatcggcat ttccatgcca
     4201 aatcaatgaa aacaaattat ggcggttata ctccttatta ttctttgtcg gaatggatat
     4261 gttctgtatt ttggccaatt ttctgaaaga aacaccacta tatccccccc ctgatttttc
     4321 ttcattgatg gcgtgggata tagcggtatt tgtcacgtta atgatattat ttttcattcc
     4381 tttattttca ttagcagtat tgctgcctat tattaagtgc atatttttgt tgtttgctgg
     4441 atgtaatcca tagttattta ttgacatttt tgttcatcct ttttgctaaa aaaaaacaat
     4501 aacctatttt tgtaatgtaa tagatattag tctattgcta atctaaaaaa accattgcca
     4561 cattgaatgg ctatattcac caataaattc tttcgatttc gtatattgac atatcgtaga
     4621 tatatcttat ccctaagatt caataatcga gtccgtttac cattctgtgc aagcgccttc
     4681 actcagaagt gaccgtcaag gcgatcacca aacttgataa taaagcagct cattgccata
     4741 cgccagtcct taaggggcgt tgtccatttc tgtgatgctg cctagattta actgccagct
     4801 ccggcttatc tacatctaaa tatgtgagcc taataccgct acactttgtc cagactatgt
     4861 ttttctccgg gaagtgggct aaccgtctcc ataaagtgac gaactttttt caacagtggc
     4921 catattgaac ggcactgatg attacgggtt attgtctcat gaagagccgg ccacatcact
     4981 gacagcagtg acttcaggcc ttcagcacca gacagcctga accagttaat ccatcgcccg
     5041 acaaatttag taatctaggg ctggtttttc ttcagctgtt gcggttcgaa agtgccatta
     5101 cgatcacgca gagtacggat ttccagaggt tcatctccag tagtgataat cttcgttaag
     5161 caaccattgc gggaattgct gccagatcta agctgatttt tctcgcaccc aagatgatga
     5221 gtcatattag cattgagtac ggtttcattt tcagcatcca atcaaactga ctgagatctt
     5281 caggattttt gagacttttg gctagttcat tagctaaagc ccgcaactta ttagatgtca
     5341 actgtttttc attcataaat tcaccttcat ttgatatcaa attaaatagt tcaacatcag
     5401 agaattatac aaacttatgt acaggctcct atgatcttcg aatcctgaag attatagtga
     5461 aaataagttt tcacgttgaa atcccacata gttagtatat gataaataat catggatgac
     5521 ctgattaacc tcttggggta gtgcttttat agaagaataa atattctact aatttaatac
     5581 atcacctaaa atagtattgt tttttatggt tggttgaagg tcgtggtgat gatgttagtc
     5641 cttttgagaa tattccagtg ccagatctta cttataataa aggggcttgg cagtttggat
     5701 aaactattgt tcttattcca ctaatacaaa ctttttgtaa agtatattaa atatacttca
     5761 tttcatactg aaatccccgg aagtattcaa tcaaacaatt acaccataaa aggaagaggg
     5821 ggccctaatt aagttgtcaa gcatgtttat gatacctgca gggtataaat tttctggctg
     5881 caagcagaca catataaaac gtctttattg atcctaccca cataatatgg acacagtcgt
     5941 aagtgaggct ctggttttca aattgttccg gactgagacc accacaccaa cggtgccgtc
     6001 gccagcgatt gtaatcgcac tcgatataac taaacactgt tgcccgcact atttcccgat
     6061 ggataatagt gttctccgtg gagacattcc attttcagcg aatgaatttt catctgttgc
     6121 tgtatccagt catacagtga ctgcatcagt ggcgcggctc tggcttttct tgccgccaga
     6181 cgctgttctg ctgaacatcc ccggacttct gcctcgatgg catacagttc accgatacgc
     6241 tgcagggctt ccgtggtgat gtcggttggc actcttgcat gcacatcgtg gatttttctc
     6301 cggacatgag ccatacatgc ggcttccgtt attctgccgg attcgtacaa cgcccggtaa
     6361 ccaccgtaag catcggcctg aagcactccg ctgtaaccgg ccagatgatt ctgtggatgg
     6421 atacctttcc ggtccggtga gtacgcgaac cagaccgccg gaggcatttc cgaaccggcg
     6481 ttacggtcat cacgaacgta gacccacagc cgggcagtcc ttgttttacc gctgcccggc
     6541 tcctggaccg gaacggggat atcatcggca tggactttac cgggcatcag cacatactgg
     6601 cgcaggatgt catacagcgg ttccagcagt tcagcaacag cacctgtcca gcgccccagt
     6661 gtggcacggc tcagctccac gccctgacga cggtatattt ctgactggcg gtataacggc
     6721 agatagtctg catattttcc agcaacaaca tgggccagaa gccccgctcc ggcataactg
     6781 cgtgcaatgg gttttgaagg caccggtgcc tgcacgatat ggtcacaccg gcaacaggcc
     6841 agtttcggac gttgtgtttc gataacctta aaggcgctgc tgataagctc cagctgctct
     6901 gacacatcac atcccagaga actgagttcg ccaccacagg ccgggcagca ttcctcttcc
     6961 ggacggatga cccgggtttc acggggaagt gaggctggta acggtttacg ggctgaagac
     7021 tggcgcaggg gagatggcag taccgggtca tattgctcac ccagcgtttc cgccatttct
     7081 tcctgaagtg cgctgattcg ctcctgtgct tccagtatct gccgttcggt ttttgcgcga
     7141 agtttttctg agcttttacc gaactgcatg cgctgcagtt tcgcaaccag cgccttcagc
     7201 cggttgattt cggaagcata agccgccacc cgctgtgaga gcaggcggtt gtatttcgcc
     7261 atctggcgga tggtgtcctg ttgcgtctgc aacagtgccc gcaggcgggc attctcatga
     7321 gcaagtgagg tgtccatatc ctcactttac aacgggttat atgcggatgc cagcgcgttc
     7381 cgttcgtttc gggtgtttcc agtcgatgcc ctcaagaagc atggataact gggccggagt
     7441 aaggtgcacc ttgccgtcac gggtgaccgg ccagacgaag cggccccgct ccaggcgtct
     7501 ggtaaagagg cacagtccgt cactgtcagc ccacaacact tttatctggt caccccggcg
     7561 tccgcggaag atgaacagat gcccggagaa cgggtcatcc ttcaggacgt tctgaacttt
     7621 tgagaccagg ccgttaaagc catttcgcat atcggtgata cctgcaacca gccagatacg
     7681 cgaacctgca gggaaagata tcatcagtgg ctgctccctt ttatttcgcg gataagtatc
     7741 tgtaataacg ccggcgtcag tttaccttta agcctgagag ttccggccgg cagaaccagc
     7801 tcacagcaca gactgtcgga cggtgtattt atctgctctg gttcctgtgc cggagccggg
     7861 attttattat ccggctccgg cgttaacgtc acgggaagca gtgcaggcat attttttccg
     7921 gaaggcagca ggccaccttt ccggtattga tggcgccagt tgaagagcag gttatcgttg
     7981 attccgtttt cacgggcgat ctgcgccaca caggctccgg gctgcagtga ctgctccact
     8041 aaagcgattt taaattcata aggaaaatta ggccgccggt
//
LOCUS       DAJTSF010000149         8012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000149 DAJTSF010000000
VERSION     DAJTSF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.149"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="OO335_003711"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="OO335_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="OO335_003712"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="OO335_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6003930.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="psd"
                     /locus_tag="OO335_003713"
     CDS             complement(4815..5783)
                     /gene="psd"
                     /locus_tag="OO335_003713"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6003931.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="OO335_003714"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="OO335_003714"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6003932.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="OO335_003715"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="OO335_003715"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6003933.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7783..7858
                     /locus_tag="OO335_003716"
     tRNA            7783..7858
                     /locus_tag="OO335_003716"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7816..7818,aa:Gly,seq:gcc)
BASE COUNT         1830 a         2069 c         2170 g         1943 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagcac
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagata
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt acgcgggaat agctcagttg
     7801 gtagagcacg accttgccaa ggtcggggtc gcgagttcga gtctcgtttc ccgctccaaa
     7861 atttgaaaag tgctgcaaag cacagaccac ccaatcgcag tatttcgaat tgcgacaagg
     7921 cggcaactga acgaatcctt gggaacttac aaaagtatgt gactaaggtg agtgaaggca
     7981 gccaaggcag tagcggttcg aaagacgaag at
//
LOCUS       DAJTSF010000150         7909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000150 DAJTSF010000000
VERSION     DAJTSF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.150"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(123..953)
                     /locus_tag="OO335_003717"
     CDS             complement(123..953)
                     /locus_tag="OO335_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR6003934.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..2176
                     /gene="intI1"
                     /locus_tag="OO335_003718"
     CDS             1163..2176
                     /gene="intI1"
                     /locus_tag="OO335_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6003935.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2379..2729
                     /locus_tag="OO335_003719"
     CDS             2379..2729
                     /locus_tag="OO335_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR6003936.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2855..3415
                     /locus_tag="OO335_003720"
     CDS             2855..3415
                     /locus_tag="OO335_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6003937.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3418..6384
                     /locus_tag="OO335_003721"
     CDS             3418..6384
                     /locus_tag="OO335_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR6003938.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6451..6828
                     /locus_tag="OO335_003722"
     CDS             6451..6828
                     /locus_tag="OO335_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6003939.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7029..7688)
                     /locus_tag="OO335_003723"
     CDS             complement(7029..7688)
                     /locus_tag="OO335_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR6003940.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1753 a         2241 c         2133 g         1782 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     1801 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     1861 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     1921 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1981 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     2041 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     2101 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     2161 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     2221 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     2281 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     2341 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     2401 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     2461 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     2521 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     2581 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     2641 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     2701 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     2761 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     2821 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     2881 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     2941 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     3001 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     3061 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     3121 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     3181 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     3241 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     3301 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     3361 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     3421 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     3481 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     3541 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     3601 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     3661 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     3721 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     3781 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaagg catcgtgctg
     3841 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     3901 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3961 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     4021 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     4081 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     4141 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     4201 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     4261 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     4321 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     4381 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     4441 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     4501 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     4561 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     4621 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     4681 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     4741 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     4801 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     4861 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     4921 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4981 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     5041 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     5101 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     5161 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     5221 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     5281 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     5341 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     5401 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     5461 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     5521 agcagcccag gacggacttt ctacacccac atctccgacc aatacgcgcc attccacacc
     5581 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     5641 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     5701 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     5761 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     5821 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     5881 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     5941 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     6001 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     6061 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     6121 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     6181 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     6241 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     6301 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     6361 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     6421 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     6481 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     6541 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     6601 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     6661 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     6721 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     6781 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     6841 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6961 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7021 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     7081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     7141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     7201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     7261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     7321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     7381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7441 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7681 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     7741 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     7801 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     7861 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgt
//
LOCUS       DAJTSF010000151         7869 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000151 DAJTSF010000000
VERSION     DAJTSF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.151"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..96)
                     /locus_tag="OO335_003724"
                     /pseudo
     CDS             complement(1..96)
                     /locus_tag="OO335_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            94..418
                     /locus_tag="OO335_003725"
                     /pseudo
     CDS             94..418
                     /locus_tag="OO335_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(448..3360)
                     /locus_tag="OO335_003726"
     CDS             complement(448..3360)
                     /locus_tag="OO335_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003941.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3363..3479
                     /locus_tag="OO335_003727"
     CDS             3363..3479
                     /locus_tag="OO335_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6003942.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3843..4685
                     /locus_tag="OO335_003728"
     CDS             3843..4685
                     /locus_tag="OO335_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6003943.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4688..5776
                     /gene="capU"
                     /locus_tag="OO335_003729"
     CDS             4688..5776
                     /gene="capU"
                     /locus_tag="OO335_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR6003944.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5781..6731
                     /gene="virK"
                     /locus_tag="OO335_003730"
     CDS             5781..6731
                     /gene="virK"
                     /locus_tag="OO335_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6003945.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6796..7740
                     /gene="lpxM"
                     /locus_tag="OO335_003731"
                     /gene_synonym="msbB"
     CDS             6796..7740
                     /gene="lpxM"
                     /locus_tag="OO335_003731"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6003946.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2119 a         1762 c         1863 g         2125 t
ORIGIN      
        1 ggcatcaagc tcctgctccg acatacctgc aggcaaaggc catgaaagcc ctgatttccg
       61 gaagcgagcg aacagtccac aaactgttga acgtggtgtc agcggcggac agggataagc
      121 cctcgtcagc agttccgtca gcactgcgac agcgttcttt acccggtcaa aacagcgttg
      181 cggtgcacca cgcctgacgg atgaactgcg tgctcagggt taccacttta acgtaaaaac
      241 cgtggcggca agcctgcgcc gtcaggggct gagggcaaag gcctcccgga agttcagccc
      301 ggtcagttac cgcgcacatg gcctgcccgt gtcagaaaat ctgctggagc aggattttta
      361 cgccagtggt ccgaaccaga agtgggcggg agacatcacg tacttacgta cagatgaagt
      421 tcggttacat cctgtcagca cggaacctca cgctttttga gcggcacgac ggatcccccc
      481 cacccagtat ccgacagact tttcactgcg ttcgtaaaaa cccttcttcc ctcccagcat
      541 atccactaat tccggtgaca tatcctgcag ataagtctgc aaatcatgcc ccagatcttc
      601 cggtgactgc aactcataaa ccgcgcgcca cacatcaccc ttccttattt taagccccgg
      661 gttaagaata tactgtccgt ggttcagcct cagaaaaagc ttttttccat aaggattttt
      721 gctgttaact tcatgttgag agagctggct gctgatataa ctccttcgtt tgcggcgttc
      781 cggcagcaac gcttcaggaa gatgaaccac acactctgcc agtcgtgccg cactgaaggc
      841 ttccgaaaaa aacagagcac ttcccagatg cgtgtggaac agcgcctgca tcaggatcac
      901 cagaaacatt gccatctgcc ggttgtccag tttgatgagg cgtccttcca cctgcagtga
      961 cagactttca ggtttcagca gggtatacaa ctgcgcactt ttctgttgcg cataacgggg
     1021 cgtactgacc gccgctgaca gaacttgatg ataagccgcc agcccgtcat taccggtcag
     1081 caagggatct gcaccacgct ccagcagtaa ctgaaccagt gcaatattac ctgcccgggc
     1141 tgctgacatc agcggagtaa gattaaacgg cgaacggtga tcaacaccat aacgttcaat
     1201 atcccgcaac acggcaaccg ggttattcat tgagtatggc cggaaatact tcagctccag
     1261 taactgcaga gtcttcgttc tgggctgcct tgtgggctta aaatcttccc ggtacagtgc
     1321 attatacagc gggaacaggg aataaatcag actgtactcc agcgcctgca gttgtatggt
     1381 tttatcggct gtacctgcga ggatatgctg tttccactgt cgtgcatcct ctgcggtgat
     1441 aatctgccat ggcatctcag atgtctgcag gatccggcta cggatggtgt ccgcctgctc
     1501 ctgcttcccc tgtttttcaa gcagatgtgc ggttttctgc cactcatcac ggctggaaat
     1561 ctcttcctgc agattcagag gctcttcctg atgcgtgagc gcaagcagac tccacagagg
     1621 atgctccacc tgctgttcaa caagatacac attgtgttta gcacgggtga gcgcaacata
     1681 cagcgcgttg gtaaaaaatt tatagatttc cgctgaacga tcctgcctgt cccgcggacg
     1741 ggaataacgg gcctcgccct caagatcagc cggtgtaaga ccctcgcaaa tatcatcaaa
     1801 tgcctgccgg gcagcgctta ctatattgta aaggatcacc gtttcgtatt ccagcccctt
     1861 aacctcctga accgaaaaga ccagcggcgt actgaaccag catcgcgcct gtgctttctg
     1921 ttccggatgc atcacaatga ccgctacacg attcgaaagt gaggttttgg cattcagttc
     1981 ctgctttgtc tcttccctat cgtccagcaa ccggatggtc ccttctgcct gaccgcagct
     2041 tctgacaaaa tggtgacttt cccgatcaat agcactaaag cggacctgtt tgagtctcag
     2101 caaccggtta gccagtgcag tgacgtggtg cccgttacgg taattcgcct gcaggatatt
     2161 aacggttgtg tcgtttccct gttgctgccg gaaaaagaga cttttgagcg aactccatga
     2221 gaaaaagctc ggatgaacta tctgattggc atcaccacag agtaaaaact gaccgggatg
     2281 acgtagcgtt ttcagtacca gttgcagttg aacaggcgtc atatcctgaa cttcatcaac
     2341 aaaaatgaca tcataacggg gagttgccaa cgaaagatac tgatgactga gcagattact
     2401 gtcatactga tgcgactgtt tcagccatgc gatataacgt tcaaacagga catagaccgt
     2461 cgggcggtcc tccatgccat agagcgactg ccgtatcccc agtgacagat acgcctcccg
     2521 ggacagcggc ccattcccgg aggcaaccgc accgatcacg cccctgaact cctcatacag
     2581 ggtatgggca gcccccagtg cggcgatagc cctgttgcgg ggcagccagt cgctgaacgc
     2641 actgaaagtc acctccctgc cttccggaat gcgcagagtt tccagaaatt ccgtcagcga
     2701 aagaaagtca atattctgta cctcactccc ttcccctgaa gcgtcataga gagtacgcgc
     2761 tttttccacc agaaatgagg aaaggctgag atacagtata tcaccagcag cctgcttcat
     2821 tttttccagt accagcgccg tcttaccact tcccgccgag ccaacaatca ccagcggtag
     2881 cgggtgttcg tacagtgcct gctgatcatc atcaaaggac agcattttat ccaggcggtg
     2941 gaaacgccca tgagacggat tgatatacgt cagcgcttca gtcgcgatat caacaggatc
     3001 cggcactggt tgttgttgta agcgcccttc atcaatcacc acgtttctgc gaagaaaacg
     3061 tgaagtgtta taggcatgat tgcgaatata ttccagaaca aggcagatag tttcaccgtg
     3121 gtagcgatac agcgaaaaca gtaatcggtc ccggatactg agccgggcgc ggtaaagatt
     3181 ggtgtcaatt ttacgaacat cagcctgaac aaagttatcg gtggcaatag cctgtgcaaa
     3241 ttttttataa cccgggatcg tgtcaggatt cagagtatga taaagacaga ttttcatggc
     3301 tgccggtttc cgtcgtgcaa aaaacatgct gattttacga aggaatgcct tactgaacaa
     3361 ccttgcccgg aatacccccc gaaatcagat ggaacaatca gaaaaatgct ctgcagttat
     3421 ggaataaaga aggtggggtg gtacgccggg agggcgatcc gctggaaatg gcgcagtagc
     3481 tgcaggcctg tcatgatgca ggaatagaag ttcgtgtaca ggttaatgaa agcgtcaccc
     3541 cgtaaataaa ctctgcgctg aaattccttt ttttcattgg agtacctgtg ttgttctgag
     3601 gtgaacatat cacctctgtt caggtagcca aattcagtgt gccacctcaa ggttcttagt
     3661 gagtcgtttt taaaagatat gataatatat cattgtttat gaattgttga tgtttaattt
     3721 agtaaattat atcaaatatg ccatgggttt gctatgttat tcatccagta tctggaaata
     3781 aaatgcacca gctttattgg gttttccttt tgtctctgct tgctaggtgc cagaaacttt
     3841 aaatgttaaa cgaagggggc attttgttca aagccaatca tgttccggta ttaatgtatc
     3901 atcatgtcag tcattgcccc ggactggtta cactttctcc cgtgacattc cggaagcaaa
     3961 taaaatggtt ggctgaaaac aactggaaaa cactgtcatc agatgaactg gagttttttt
     4021 atcggggcgg aaaactcccc cggaaaagcg ttatgttgac atttgatgat ggataccttg
     4081 acaactggtt tcaggtatat cctttattaa aagagtttaa tctcaaagca catatttttt
     4141 taattaccgg ttttattggg aatggtccgg ttcgtcactc tcctggaaag gaatattctc
     4201 atcgcgattg cgaacatcaa atagcgacag ggaatgctga taatgttatg cttcgctggt
     4261 ctgaagtaaa cgaaatgttg caatctggtc tggtcgaatt tcatgttcat acgcatactc
     4321 atacacgctg ggataaaaag ttcagttcac gagaagaaca gtgtaaacat cttcggcagg
     4381 acttgttgag cgggagggaa tatttgaaag aaatgacagg aaaatgcagt aaacatttgt
     4441 gttggccgga aggttattat aacaaggatt atattcaggt cgctgaagaa cttggattct
     4501 attatttata tacaacagaa agaagaatga atgctcctgc taaaggaact acacggatag
     4561 gaagaataag caccaaagaa cgggaaagtt gcgcatggct gaaaagacga cttttttatt
     4621 atacaacccc gtttttttca tcgttgctgg cctttcataa ggggccaaga ttaccggatg
     4681 attaataatg aatatactat ttacggaatc atcaccgaat ataggtggtc aggaattaca
     4741 ggctgttgct caaatgaagg ccctgaagaa aatggggcat tcagttctgc ttgtctgcag
     4801 ggaaaacagc aaaattgctt ttgaagccag taaattgggg attgatatca cattcgcgtt
     4861 atttcgaaac agtcttcaca tccctactgc atggagatta ctcggaatag ttcatggttt
     4921 tcagcccaat gcaatcgttt gtcacagtgg gcatgatagc aatattgttg gtttagtacg
     4981 gttattcact aggaaacatc cattcagaat tatcaggcaa aagacatatt tgacacgaaa
     5041 aacaaaagtt ttttcaataa atcatttttg cgatgaggtg attgttcccg gaacaagtat
     5101 gaagacacat ctggagcagg aaggatgtcg aacccgggtt actgttgtgc ctccaggctt
     5161 tgatttccag aaattatacg ttgattcccg aaacagtttg cctccaaatg ttctttcttg
     5221 gctggcgtcc cgaaggggat gccccgttat tgctcaggta ggaatgttgc gcccggaaaa
     5281 agggcacgaa tttatgttga atttactgtt ccatttaaaa atgaatggac gacagttctg
     5341 ttggttgatt gtggggtctg gttcgcctga actgcgggag catttacagt atcagattga
     5401 cagtatgggc atgcatgatg atgtttttat tgctgacaat gtttttcctg ccgcccccgt
     5461 atatcgggtt gccagtctgg tggttctgcc ttcagaaaac gaatcgtttg gtatggtact
     5521 ggcagaagca tcggcatttt ctgtgcctgt actggccagt cagattggtg gaatccctga
     5581 tgttattcag aataaccaga ccgggacatt gttaccagca ggtaataagc acgcatggat
     5641 gtgcgccctg aatgattttt ttaatgaccc tgggcgtttt tatcagatgg ctcgccaggc
     5701 aaaacaggat atagaagagc ggtttgatat taataaaact gcgttaaaaa tactcacatt
     5761 agcgaagcac aagtaacaat atgttttctg taagtaactt atcatttatc ggtttcctta
     5821 aaaggattgt tttttcctca gattcactcc cggggaagtg ggaacacaga aaattccggt
     5881 tcatgtacat tttgcgatgt gctataaatc cggttgccag tattcgatat tattacgaac
     5941 tgcgttcctt gcagtgcatt gaggatattc tggcaataca acccacgttg ccagcaagaa
     6001 ttcatcgacc ttatttgcat aaaggggggc gagcctggtc ccgggggcaa tatattcttg
     6061 agcattatcg ctttgtgcaa aatttgccag aaaaatattc tgaatttttg tttccacaaa
     6121 aatcggtatc attagtccaa ttcatcggga aagatggaga ggattttgat atccagtgtt
     6181 ctcccagtgg ttttgaccgg gaaggagagt tgatgttgtc attgtttttc aataaaatag
     6241 tgattgcccg gttaacattt tcagtcattc tgactcaaaa tggacacacc gcttttattg
     6301 gcggattaca gggagccccc aaaaataccg gacctgatat tattcggtgc gcaacccggg
     6361 cctgttatgg gcttttccca aaacggatta tttttgaagc attttgtgcg ttgatgaaag
     6421 cttgtaatgt ctctgagtgt ctggcagtca gtgagcacag tcatgttttc cggcaattga
     6481 gatactggta tcagaaacgc aaaacctttg ttgctgtcta cagtgatttc tgggagtctg
     6541 tagcagggaa aacctgtggt gactggtaca aattaccaac acaggtggtt cgaaagccgc
     6601 taagcaatat agctagtaaa aaacgctctg agtatcgaaa acgatacgct ttgctggatt
     6661 atatccatga aactgctatc cgctctttgg atgcatatcc tgtaaactca gaacatcagg
     6721 acttaaatta aggtttgatg tattctctga ataaaatatt aatgatgatt ttggtagggg
     6781 cattcgcact aaataatgaa aaaatacaaa tccgagttta ttcctgaatt taagaaaaat
     6841 tatctttccc ctgtttactg gtttacatgg ttcgttttgg gaatgattgc aggtatttca
     6901 atgtttcccc cttcattcag agatcctgtc ttggccaaaa tagggcgttg ggtgggcaga
     6961 ttgagcagaa aagctcgtcg cagggcgacg attaatttat cgctttgttt cccggaaaag
     7021 agtgatacag aacgggaaat aattgtcgac aatatgtttg ccacagcatt gcaatccata
     7081 gtgatgatgg cagaactggc aattcgtggt ccggaaaagt tccagaaacg agtgttctgg
     7141 aaaggcctcg aaattcttga ggaaattcgg cacaataaca gaaatgtgat ttttctggtt
     7201 ccccatggct ggagcgtgga tattcctgca atgttgctgg cagctcaggg ggaaaaaatg
     7261 gccgccatgt ttcatcagca acgaaatcca gtgattgatt atgtctggaa ttctgtacgg
     7321 cgtaaattcg gggggcgctt acattcccgg gaggatggga taaaaccatt tattcagtca
     7381 gtacgccagg gatactgggg gtattacctt ccagatcagg atcatggtcc tgaatacagt
     7441 gaatttgctg atttttttgc gacctataaa gcgacattac caattattgg acgtctgatg
     7501 aacatcagtc aggctatgat tataccgctt ttcccggttt atgatgaaaa aaaacatttc
     7561 ctgactattg aagttcggcc accaatggat gcatgcattg ccagcgcgga caataaaatg
     7621 attgcccgac aaatgaacaa aacagtggaa attttggtgg ggtcacatcc ggaacagtat
     7681 atctgggttt taaaattgtt aaaaacgcgc aaatcaaacg aagcggaccc gtacccttga
     7741 aattataaag tacaggtatt tccactagtt gtttcttaca ggttaccaat cgaaacacat
     7801 cccccttccg tgtgttgccc gtttggcacg gaggactgtg tagatgcagt tcgttaaatt
     7861 taatttatg
//
LOCUS       DAJTSF010000152         7743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000152 DAJTSF010000000
VERSION     DAJTSF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.152"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="OO335_003732"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="OO335_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR6003947.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="OO335_003733"
     CDS             1102..2562
                     /locus_tag="OO335_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6003948.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="OO335_003734"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="OO335_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6003949.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="OO335_003735"
     CDS             complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="OO335_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR6003950.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="OO335_003736"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="OO335_003736"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6003951.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="OO335_003737"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="OO335_003737"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6003952.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="OO335_003738"
     CDS             complement(6727..7245)
                     /locus_tag="OO335_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR6003953.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7521..>7743
                     /gene="ydeI"
                     /locus_tag="OO335_003739"
     CDS             7521..>7743
                     /gene="ydeI"
                     /locus_tag="OO335_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR6003954.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHK"
BASE COUNT         1940 a         1812 c         1956 g         2035 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc gccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa
     7321 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     7381 tgagtcaaat tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa
     7441 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
     7501 aacgcaatta aggagttagt atgaaatttc agtccattgt attagcaagt tttcttgtca
     7561 tgccttatgc gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc
     7621 ctcatgcaat agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt
     7681 tcgcaaaaaa catgcacgat ggcgcttcgg tttcactacg cggtaatttg atttctcaca
     7741 aag
//
LOCUS       DAJTSF010000153         7721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000153 DAJTSF010000000
VERSION     DAJTSF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.153"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..832)
                     /locus_tag="OO335_003740"
     CDS             complement(<1..832)
                     /locus_tag="OO335_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCR6003955.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     E"
     gene            1014..2438
                     /gene="sbcB"
                     /locus_tag="OO335_003741"
     CDS             1014..2438
                     /gene="sbcB"
                     /locus_tag="OO335_003741"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6003956.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2481..2708)
                     /locus_tag="OO335_003742"
     CDS             complement(2481..2708)
                     /locus_tag="OO335_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6003957.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2722..3780)
                     /gene="yeeE"
                     /locus_tag="OO335_003743"
     CDS             complement(2722..3780)
                     /gene="yeeE"
                     /locus_tag="OO335_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR6003958.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="OO335_003744"
     CDS             complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="OO335_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6003959.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="OO335_003745"
     CDS             complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="OO335_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6003960.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5530..6513)
                     /locus_tag="OO335_003746"
     CDS             complement(5530..6513)
                     /locus_tag="OO335_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6003961.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6559..7383)
                     /locus_tag="OO335_003747"
     CDS             complement(6559..7383)
                     /locus_tag="OO335_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6003962.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="OO335_003748"
     CDS             complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="OO335_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR6003963.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2067 a         2012 c         1812 g         1830 t
ORIGIN      
        1 gttcgaagat tggacggcgg ataacattac ccatcttgct atgctctttc ggtacggtcc
       61 atacgttgtc cagcaggtca aattctgtct ttgttgccag cctaagctct gagagcctcg
      121 ccccccacag cataagcatc tgatgaagga gcttatttga cgtagacgca cggcttcttt
      181 caatagcaag ccaaatctta gccagttcgt gatacgacag tacccgatcc cctacctcag
      241 cgcgggagcc gaagtccctt ggctggatgc tcataattgc gcaactatct atcaactgac
      301 gccgcatgca ccaactaatt gctgatctta gttgacttag cacctgcctt gctcggcgtg
      361 gattatcttt ttcttcttcg gtaagcaggt caacccattg cttaaccgtg atagaagatg
      421 ccggacgatt agggaaggcg tcatgcatgc gcttcataac cgttgatcga taaagtgcct
      481 gggtcttttc tctgagagtt gtagagacgt agttgtcgaa ccagtagtcg agacactggg
      541 cgaccgtcat ggagttctcc accttctctt caaaataggt gcgtggatcc gttcctgaga
      601 aatagagctt tcgcaagtca gcagtgatct gtctggcatc cttcaaagac agggatgggt
      661 atcgaccaag cccaagtcga ttaggcttgc catgccagcg atagcggtac tggaactgga
      721 tgaccccctt cggtgaaatt cgtacgctga ggccatcggc atcagccact tcttgtgggc
      781 ccgaatatgg tttaccataa atagtacgca gttttgtgtc gcttatagcc ataaataata
      841 tttggtacgc tcagaaataa tagtttggca cacatctggt acacaatatc acatggacaa
      901 cagcgcacag taaacaacta tatttgacgt tcgtagacat actcaggtga aaaaagggtt
      961 gcttttcgag ttataacaga cagttaatta actacagtgg acaatgctag acaatgacag
     1021 acacagataa acaacctacc ttcctctttc acgattacga aacctttggc acgcaccccg
     1081 cgttagatcg ccctgcacag ttcgcagcca ttcgcaccga tgacgaattc aatgtcatcg
     1141 gcgaacccga agtcttttac tgcaagcccg cggatgacta tttaccccgg cctggagcag
     1201 tattaattac cggtattacc ccgcaggagg cacgggcgaa aggagaaaac gaagccgcgt
     1261 ttgccgcccg tattcactcg ctttttaccg taccgaagac ctgtattctg ggctacaaca
     1321 atgtgcgttt cgacgacgaa gtcacacgca acgtttttta tcgtaatttc tacgatcctt
     1381 acgcctggag ctggcagcat gataactcgc gctgggattt actggatgtt atgcgtgcct
     1441 gttatgccct gcgcccggaa ggaataaact ggcctgaaaa tgatgacggt ctaccgagct
     1501 ttcgccttga gcatttaacc aaagcgaatg gtattgaaca tagcaacgcc cacgatgcga
     1561 tggctgatgt gtacgccact attgcgatgg cgaaactggt aaaaacgcgt cagccacgcc
     1621 tgtttgatta tctctttacc catcgtaata aacacaaact gatggcgttg attgatgttc
     1681 cgcagatgaa acccctggct cacgtttccg gaatgtttgg ggcatggcgt ggcaatacca
     1741 gctgggtggc accgctggcg tggcatcctg aaaatcgcaa tgccgtaatt atggcggatt
     1801 tggcaggaga catttcgcca ttactggagc tggatatcga cacattgcgc gagcgtttat
     1861 ataccgcaaa agccgatctt ggcgataacg ccgccgttcc ggttaagctg gtgcatatca
     1921 ataaatgtcc ggtactggcc caggcgaata cgctacgccc ggaagatgcc gaccgactgg
     1981 gaattaatcg tcagcattgc ctcgataacc taaaaattct gcgtgaaaat ccgcaagtgc
     2041 gtgaaaaagt ggtagcgata ttcgcggaag ccgaaccgtt tacaccttca gataatgtgg
     2101 atgcacagct ttataatggc tttttcagtg acgccgatcg tgcagcaatg aaaattgtgc
     2161 tggaaaccga gccgcgtaat ttaccggcac tggatatcac ttttgttgat aaacgaattg
     2221 aaaagttgtt gttcaattat cgggcacgca acttcccggg gacgctggat tatgccgagc
     2281 agcaacgctg gctggagcac cgccaccagg tcttcacgcc agagtttttg cagggttatg
     2341 ctgatgaatt gcagatgctg gtacaacaat atgccgatga caaagagaaa gtggcgctgt
     2401 taaaagcact ttggcagtac gcggaagaga tcgtctaatt tgaagccgcc gccgctggta
     2461 cggcggagga caatgttgag tcaggctttt tgaacggtga tgctccacgc tgcatcgcca
     2521 atctgctgat aatcggtgat ggcatgtcct tcttctgccg cccactgtgg gattgcttca
     2581 gttgcctggg tgcaatcaaa ctcaattacc aactcatcgc cgctggccat ttctgccagt
     2641 gctgcttttg cttctatgag tggaaacgga cagacctgcg tcaccacatc cagcttttta
     2701 atcgccatct ttacacctta cttaatttgc cgcagcagtt gccagtcgcg ccttacgctg
     2761 cggtcgaaca tacacaagcc aggacgcagt ccagactccg agaatcataa atacaaggcc
     2821 aatccagccc tgccaggtca tcattgcagt cataaccagt ccattaccga tagagcaacc
     2881 acctgcaata ctggcaccga agcccatcag tacaccacct aacccgctac gtaatgttgt
     2941 ttgtgcatca gctgcgcgaa cgcggaactc acggctcgct ttggctgcaa taaatgcctc
     3001 cacgaagatc cctaacacca ggaaaacgcc ccagttaatg tatttcacgt cacccgcgac
     3061 cagaaattga agaatattgg ccgttgggga agtgactcct aacccaaaca tgcgtccggt
     3121 tgcttcactc aggggccagg ctaaaagcgc aatcaaaccg atgagtacag cagtgacaaa
     3181 gggatgccag cgtttttcaa acagaatatg agcgatcccg gttcggcgcg gcggtaaggt
     3241 cgcgactttg agttttggct tcttcaactc tttcatcacc acccagagcg ttatcaccag
     3301 caaaacggta accaacggcc acacagacaa attaaaagtc tcagcaatag agttatgttc
     3361 agtagtgtag tgctgcaagg tttgatttaa accactggcg tgtggagaac gcatcaccgc
     3421 actcatcacc atataagtga aaagcgcgat ccaactgccg atcaatccct cacccgcgcg
     3481 ataccaggtc ccggtggcac atccacccgc cagaacaatt cccagcccga aaagataccc
     3541 acctataaca gtaccaagcc acgggaacgc cccggcttcg taagtcagta ggcccgcctg
     3601 aattaacgca aaaaccccca cgctttgtgc cgaaattgca atcagcaagg cgtaaaacat
     3661 gcgattattt ttcgcgatat acatatcgcg aaaaccacca gtcaggcaaa aacgcccacg
     3721 ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca ttgaaaacat
     3781 aaattaatta accagatgaa tgttaatggg gaaatttttc atgactggtg gaatgcagac
     3841 caataaccaa attctctaaa ttagaacaaa tggttattaa tgaggatttc gataaaaaag
     3901 aataaaaaaa ccggagccaa atgttcattc gactccggtt tattattaga aaagatgatt
     3961 atgctacgtc ttcgtactgc ggaaccggat tacggaagct cttggtgacg caagccaggt
     4021 aaatcagacc gatagcagcc cagatcagac ccagaaccat tgagctttct tccaggttaa
     4081 cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac tggaagtgat
     4141 ctttcagcgt cttgttacgc ttctcacgga tccagaactg cgagatgacc gacaggttaa
     4201 caaaggtgaa cgccaccagc gcaccaaagt taatcagcgc cgttgccatt accaggtcga
     4261 agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc ggagtacgcc
     4321 atttcgggtg tacataaccg aagaagcttt tcgggaacac gccgtcacga cccattacgt
     4381 acatcagacg cgctacgcct gcgtgcgccg ccataccgga cgccagtacg gtaatggtgg
     4441 agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg atttcaggct
     4501 gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc aggaagtaag
     4561 ttgcaaagat gaagatcatg ccgccaatca gcgcggtcag gaaaatcgca cgcgggatca
     4621 cgcgctctgc atctttggtt tcttccgaca gattgctgat gccgtcaaag ccggtaaagg
     4681 agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg ccagaccaga
     4741 acggacgcgt gctcgccagc gtaccagcgc cttcaccttc aaatacgcca taaacaacca
     4801 tgcccagaat caccgcgatc agcactacct gcaacacgac gattacagtg ttgacgttcg
     4861 ctacggattt cagactacgc aggttaaagg cggtcataaa ggccaccagc gccaccacaa
     4921 acatccacga tggaatggaa ggcaccagag cttcaaaata gattttcgcc agcagaatgt
     4981 tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata aagccgacag
     5041 tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga tagcggcgaa
     5101 ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac gcataggctg
     5161 tcggcacatg accatccgtg aggcctgaaa cgataccaaa tgtatcaaac agcgtcatcg
     5221 gctgcatata ggctagaccc atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt
     5281 ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt tattctcctt tacggtgata
     5341 accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac aacaactgtc
     5401 gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa tgattggttt
     5461 gaagcaaaaa ataaccgacg ctaattaaac gtcggttttt aatcattttt tgacagcggc
     5521 gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca taataatttt
     5581 tctttgaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga tcgcactatt
     5641 tgctaaaatt tcatctcgcc accagccgct ggcaagccct gcggtctgtt cattccagcc
     5701 aatccagaca ggttcaaaga gagtttgggc gaccactttc ttctcgatta acgcgccact
     5761 atcgagaaaa cgttgcgcca gataccgtgg taaatagccg cagccaaggc cgctaatttg
     5821 cagctccagt ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt cgagaagttg
     5881 tgaggccgta gaagcgcctg cctgcgcagt atcccccacc acaatcgccc gatatcgttt
     5941 aatgatacga cggtttaacg gttcttcttc ctgggctaac ggatgatgag gcgcgaccgc
     6001 aaagacttgt tcaagatcgc ccaaccgcga gaaaccaaac tcactggaag atggtggctc
     6061 atgcatcgcg ccaacgataa tatccgctcg cccctgagtc agggcatccc aggaaccacc
     6121 gagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa aagcttcaat
     6181 gagaggcgca agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca gctcgttttc
     6241 ccagccttca tgcagtttga tcgcctgctt ttccagttct cgcacggtat gcagaacttc
     6301 ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt gtccgctacg
     6361 atcaagcaat tgaatattta ggtcgctttc cagcttgtga acggtgtaac tcagagcgga
     6421 cggcgtcttg tagagtttgg ctgacgccgc cgcaaaactg ccctcttttt ctatggcatc
     6481 gaggatcatc aacacatcca gcagtggttt catacacgcc cccttgcagt gcatgcggcg
     6541 tcctgctggc gtgatcgttt actccaacgg cattaccagc ggatcgggat actggtattc
     6601 aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc cgctgtccag
     6661 actatttctg aactgcggag gttgcagccc cagtaaacgg gccatctgcg gatagaaaac
     6721 attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt taggtgcctg
     6781 taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa caccatgttc
     6841 accatcaggg gcggtttttc cggcaaagaa gcgtcccgga tgacgtcccg gtccgaccag
     6901 gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc agtcttcgag
     6961 ttcttctaat actcgtccac tgttggttac cggattacgc ggggtagttt ctttaaccgt
     7021 gccttgcgcg tcgccataga cagatgttga gctggtaaaa ataattcggg gaatacgatg
     7081 ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat cgccggggcc
     7141 gctacgacgt gccggaagcg taatgaccag cgcatcggca tccataaggg catccagatc
     7201 gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa tgccactcat
     7261 tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc agcctcgcgc
     7321 tgaaagtgac atcgccagcg gcatacctaa ccaccctaac ccgacaattg cgaccttttt
     7381 catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta tcaaaactga
     7441 caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca atgagcagtg
     7501 aatcatcagt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg gaccagaaac
     7561 ctgacaaatt atgtttcagg ggttctggtt tccccttacc ttcaaatggt gttctgcggg
     7621 tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc tgccagtaca
     7681 gataatcatc ccatgattcc tcagaccaga ttagtttcac t
//
LOCUS       DAJTSF010000154         7536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000154 DAJTSF010000000
VERSION     DAJTSF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.154"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="OO335_003749"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="OO335_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="OO335_003750"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="OO335_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6003964.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="OO335_003751"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="OO335_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="OO335_003752"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="OO335_003752"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6003965.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="OO335_003753"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="OO335_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6003966.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO335_003754"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO335_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6003967.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="OO335_003755"
     CDS             complement(6510..7202)
                     /locus_tag="OO335_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6003968.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1915 a         1828 c         1986 g         1807 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ccggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtgt tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccgggat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagaaaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcaccccc ccccgcgccg ctgagaaaaa gcgaag
//
LOCUS       DAJTSF010000155         7374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000155 DAJTSF010000000
VERSION     DAJTSF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.156"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            51..1541
                     /locus_tag="OO335_003756"
     CDS             51..1541
                     /locus_tag="OO335_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6003969.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1593..2750
                     /locus_tag="OO335_003757"
     CDS             1593..2750
                     /locus_tag="OO335_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6003970.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="OO335_003758"
     CDS             complement(2716..2838)
                     /locus_tag="OO335_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003971.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3041..4726
                     /locus_tag="OO335_003759"
                     /pseudo
     CDS             3041..4726
                     /locus_tag="OO335_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4764..7124
                     /locus_tag="OO335_003760"
     CDS             4764..7124
                     /locus_tag="OO335_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6003972.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7181..>7374
                     /locus_tag="OO335_003761"
     CDS             7181..>7374
                     /locus_tag="OO335_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003973.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGS"
BASE COUNT         2163 a         1615 c         1567 g         2029 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     2941 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3001 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3061 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3121 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3181 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3241 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3301 aatatccgca aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3361 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3421 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3481 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3541 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3601 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3661 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3721 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3781 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3841 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     3901 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     3961 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4021 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4081 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4141 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4201 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4261 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4321 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4381 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4441 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4501 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4561 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4621 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4681 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4741 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4801 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4861 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     4921 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     4981 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5041 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5101 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5161 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5221 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5281 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5341 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5401 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5461 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5521 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5581 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5641 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5701 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5761 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5821 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     5881 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     5941 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6001 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6061 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6121 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6181 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6241 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6301 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6361 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6421 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6481 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6541 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6601 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6661 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6721 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6781 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6841 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     6901 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     6961 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7021 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7081 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7141 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7201 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7261 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7321 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt catt
//
LOCUS       DAJTSF010000156         7188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000156 DAJTSF010000000
VERSION     DAJTSF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.157"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            28..879
                     /gene="hchA"
                     /locus_tag="OO335_003762"
     CDS             28..879
                     /gene="hchA"
                     /locus_tag="OO335_003762"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6003974.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(987..2345)
                     /gene="hprS"
                     /locus_tag="OO335_003763"
     CDS             complement(987..2345)
                     /gene="hprS"
                     /locus_tag="OO335_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6003975.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="OO335_003764"
     CDS             complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="OO335_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6003976.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3149..3562
                     /gene="hiuH"
                     /locus_tag="OO335_003765"
     CDS             3149..3562
                     /gene="hiuH"
                     /locus_tag="OO335_003765"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6003977.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3671..4675
                     /gene="msrP"
                     /locus_tag="OO335_003766"
                     /pseudo
     CDS             3671..4675
                     /gene="msrP"
                     /locus_tag="OO335_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4676..5311
                     /gene="msrQ"
                     /locus_tag="OO335_003767"
     CDS             4676..5311
                     /gene="msrQ"
                     /locus_tag="OO335_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR6003978.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5569..6219
                     /gene="zinT"
                     /locus_tag="OO335_003768"
     CDS             5569..6219
                     /gene="zinT"
                     /locus_tag="OO335_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6003979.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2032 a         1629 c         1507 g         2020 t
ORIGIN      
        1 taactaagca acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc
       61 gatattgctg aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt
      121 cccgtctctg atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt
      181 ctggtgatcg ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc
      241 ggtaaccatc cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa
      301 ttcgaagtgg cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac
      361 aaagatgaaa aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag
      421 aaactcgcgg atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt
      481 cctggtgggc atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta
      541 cagtgggcaa tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt
      601 ctggcacttc gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac
      661 gccgcagaca aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc
      721 ggcgaagagt tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta
      781 cataaggacc gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa
      841 ctggcggcgc aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta
      901 agcgcataat caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt
      961 aacttatggc gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg
     1021 ttatgcttac tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg
     1081 actaaagaaa ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat
     1141 ctacggaaga gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta
     1201 agagagccgt tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat
     1261 cgaatggcgt taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag
     1321 atttgttgat tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga
     1381 tagtccaaaa gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca
     1441 ttgtttttgt ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc
     1501 agctcttcaa tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg
     1561 agcgtaacct gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga
     1621 tcgtcagcaa attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc
     1681 aacgcctgcc caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg
     1741 ctatcgttat aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc
     1801 gtcctgatta acaacggact taatactgag cacagtataa tggcgacaat gcaaattata
     1861 atgctattaa ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca
     1921 gtaaccgtta atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta
     1981 tcattaataa tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta
     2041 tttaacatgt catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga
     2101 atgatcaaaa tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct
     2161 ggatttaccc catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta
     2221 tcatcgcgcc atttcaactc acttgccagg ccattataga gagtccatac aattccagcg
     2281 ccagcaacag actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt
     2341 ttcattattt ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct
     2401 tttcaggaaa aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat
     2461 tggtatcact atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa
     2521 tctcgccagc tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa
     2581 tactgatatt gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt
     2641 ctaatgttga attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact
     2701 cagaaaatga aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc
     2761 tgtcatcgac agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta
     2821 acgtttgtaa gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg
     2881 cataatcatc cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga
     2941 cataacccgc ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa
     3001 taagtagaat cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt
     3061 tcattacaaa attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa
     3121 aactcattca catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc
     3181 aacggcagcc ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt
     3241 gcacattttg aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa
     3301 gaaagcggat aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat
     3361 taaggcactg tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac
     3421 cggggactat ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt
     3481 tcatattaat aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta
     3541 ttcaacctat cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag
     3601 ggaggtgact ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt
     3661 gtgatggctt atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt
     3721 attctttatg aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc
     3781 tttgcctcac gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc
     3841 cgcccccgcc ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc
     3901 actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa
     3961 agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag
     4021 cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct
     4081 ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat
     4141 tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt
     4201 cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg
     4261 cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct
     4321 cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt
     4381 gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa
     4441 gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt
     4501 ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt
     4561 tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta
     4621 cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg
     4681 tctgacagca aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc
     4741 gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga
     4801 tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac
     4861 acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt
     4921 atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt
     4981 gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat
     5041 tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa
     5101 actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc
     5161 aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct
     5221 ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg
     5281 caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat
     5341 cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt
     5401 tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca
     5461 atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt
     5521 acaatataac attacacata atgtacatta actctggagg aaattgtttt ggcgattcgt
     5581 cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat
     5641 ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt
     5701 tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc
     5761 gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca
     5821 gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat
     5881 atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca
     5941 tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc
     6001 gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt
     6061 agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat
     6121 tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg
     6181 agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt
     6241 tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga
     6301 caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac
     6361 tgagaaaaag gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt
     6421 tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt
     6481 ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa
     6541 caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt
     6601 ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc
     6661 agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa
     6721 ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc
     6781 acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct
     6841 gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag
     6901 ggggatttga acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga
     6961 gtcgaaaaat caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga
     7021 aaaatcaatg aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac
     7081 ctgttttcag gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct
     7141 cagcttattc cgttttgcct gtgtgttgaa taattccact gcagttat
//
LOCUS       DAJTSF010000157         7185 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000157 DAJTSF010000000
VERSION     DAJTSF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.158"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(12..705)
                     /gene="ospZ"
                     /locus_tag="OO335_003769"
                     /pseudo
     CDS             complement(12..705)
                     /gene="ospZ"
                     /locus_tag="OO335_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            761..913
                     /locus_tag="OO335_003770"
                     /pseudo
     CDS             761..913
                     /locus_tag="OO335_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2294..3241
                     /gene="icsP"
                     /locus_tag="OO335_003771"
     CDS             2294..3241
                     /gene="icsP"
                     /locus_tag="OO335_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR6003980.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3803..4175)
                     /locus_tag="OO335_003772"
                     /pseudo
     CDS             complement(3803..4175)
                     /locus_tag="OO335_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4419..4538
                     /locus_tag="OO335_003773"
                     /pseudo
     CDS             4419..4538
                     /locus_tag="OO335_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4788..5654
                     /gene="ospB"
                     /locus_tag="OO335_003774"
     CDS             4788..5654
                     /gene="ospB"
                     /locus_tag="OO335_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR6003981.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5983..6723
                     /gene="phoN2"
                     /locus_tag="OO335_003775"
     CDS             5983..6723
                     /gene="phoN2"
                     /locus_tag="OO335_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR6003982.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7083..>7185)
                     /locus_tag="OO335_003776"
     CDS             complement(7083..>7185)
                     /locus_tag="OO335_003776"
                     /inference="COORDINATES: protein motif:HMM:NF017898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR6003983.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2250 a         1355 c         1385 g         2195 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aatctctggc gactcatctc ttaacacttg
      181 cataatctct ctttttatat catatagttc tataaaccga tttgcccgta gttctccttg
      241 ggaagaatca tttctgaata caacaattat atacaaaaaa tcactatttt ctttagagaa
      301 gatcttacaa ctaatctctg ggagctcaca taatgcatgt aagtcaggat ggtacaaact
      361 atccacagaa aattgcccca taggacgttt tccagatata actgtcaacc agcttccttc
      421 cggtgtactg gcttcggctc tggaaatagg ctgaaaatct tccggacatg gagaatacat
      481 tgtcttcata taattaagtc gtctggattt ctcattaata aactcctcta tttttaattg
      541 tgccatactt ctggttatac cagagatata cttcttgtca tatatgtttg gtgattcatt
      601 aaagtaacca tgttcaactc taggataaat atttctcact atatattcag tatttgcagt
      661 atttattgga aatacgttct taatattctt gatgggacta atcattttaa tctctatact
      721 caaatataaa cattaccatg aacaatataa ttgttcctaa gtggaatgtc tccacggaga
      781 acactttatc tatcgggaaa tagtgcgggc aacagtgttt aattatatcg agtgcaatta
      841 caatcgctgg cgacggcacc gttggtgtgg cggcctcagt ccggaacaat ttgaaaacca
      901 gaacctcgct taggcctgtg tccatattac gtgggtaggc tcacagagcc tcacttacga
      961 ctgcgtccat attatgtggg taggatcaat aaagacgttt tatatgtgtc tgcttgcagc
     1021 cagaaaattt ataccctgca ggtatcataa acatgcttga taacttaatt ggggctccct
     1081 cttcctttta tggtgtaatt gtttgattga atacttccgg ggatttcagt atgaaatgaa
     1141 gtatatttaa tatactttac aaaaagtttg tattagtgga ataaggacaa tagcttatcc
     1201 aaactgccaa gcctctttat tataagtaag atctggcact ggaatattct caaaaggact
     1261 aacatcatca ccacgacctt caaccaacca ttttattgca tttaaatccc agaatagttc
     1321 agtagtggtc aatttgggaa acgcacaata ttgtacggtt acgttatcgc aatagagtga
     1381 actctcgccc tatacggcgt tttcaaggat tagtccttaa tcggacaacc acattaaagc
     1441 ataataaata attaagcttt aataatatac tatagataca gttctattat aaaaaatact
     1501 attttaggtg atgcattaaa ttagtagaat atttattctt ttataaaagc accaatgaat
     1561 tgtccaagta aacatgagat aaaatgtgta gaggataact gagtttggtc actttaactg
     1621 tattagtcgc agtctgactc tcgactttaa aaggatggga attacctgta ctgatagtga
     1681 gaaagctttc cattgaaata agtcaaataa aagttgatgg cttcagtgct aaagtgaggt
     1741 gcccgctagc cgtattcact cgaatcggaa ttcaatgtat tgcttctgcc atttccataa
     1801 tatttcacgg gaaaaacaaa atctgaaaaa ctatacttaa gtgttgtatt gagtcagttg
     1861 agatacaaat acatatttat cagcacacgt tacgtcaaat acaggaggtt gtcatgttgg
     1921 tactgaaagg cacgttggct attgcagccg tgatgacaat ttattgtctt atcgttgttc
     1981 ttatggaacg cttttctgat tgatttcata ttggcgagag aacaagaagg tgagtagaat
     2041 tgtcgcggaa tcctgaaact atcagcctca ggtatgaaca ccaaaaggta ggtagagaaa
     2101 tctatggccc ccgttacttt attcgcacaa tgaggtattt ttttattttt acaaaagttc
     2161 aataaatatt ataataaaag atatgttctt ggtaacataa acaaatattt aattaaaata
     2221 atgcttggca ttgacgttta tcttataaag taagaagatc atggacattt caaccaaaaa
     2281 agtagagttc tcgatgaaat taaaattctt tgtacttgca ctttgtgtac ctgcgatctt
     2341 tactacacat gctaccacta actatccact tttcataccg gacaacatca gtacggatat
     2401 tagtctagga tctctgagtg gcaaaacaaa agaacgcgtt tatcatccca aggaaggggg
     2461 gagaaaaatt agtcaactgg actggaaata cagtaatgcg actattgtta gaggtggcat
     2521 cgattggaag ctaattccaa aagtgtcttt cggagtttcc ggttggacta ctttaggtaa
     2581 ccagaaagca agcatggttg ataaagactg gaacaattcc aatacccctc aggtatggac
     2641 cgaccagagc tggcatccca atacgcatct ccgtgatgct aacgaattcg agctgaatct
     2701 taaaggttgg ttattaaata atttggatta tcgacttgga ctaatagcag gttaccaaga
     2761 gagtcgttac agttttaatg caatgggagg gagttatatt tatagtgaaa acggggggag
     2821 caggaataaa aaaggggcac atcctagtgg tgaaagaaca ataggttaca aacagctctt
     2881 caaaatacct tatattggat taactgctaa ttaccgccat gagaattttg agtttggagc
     2941 agaactgaaa tatagtggtt gggttctttc atctgataca gataaacact atcagactga
     3001 gacaattttt aaagatgaaa taaaaaacca aaattactgc tctgttgctg cgaatattgg
     3061 atactatgtc acccccagtg caaaatttta tatagaaggc tccagaaatt acatttctaa
     3121 taaaaaaggt gatacatctc tttatgagca aagtaccaat atatctggca ccattaaaaa
     3181 tagtgcaagt attgaatata ttggttttct cacttccgca ggtataaagt atattttttg
     3241 aattggttca ccgagttgat tacgttttca tggctaaaaa ttgccgccca ttgtgagtca
     3301 tattcgccct gactttccag aaccatacga actgcccgtt gacggacttc gggggaaaaa
     3361 cgtgtatttt tagtcattct gtttacctct ttctcagggc gtttagtctc caggatttcc
     3421 ggggcggttc aaaaaactct tcgttgatta ctgtggactt actgttggca ttaccgaccc
     3481 tgagaccgga gaaataagaa ccgcttagat catcgtggct gttctcgggg tatcaagtta
     3541 cacatgggca gaggctaccc aaggtctact attatcgcct gaaagcgctg atggaacagt
     3601 gctatcaggg acatgctgat gaaaatacct aaataatcag ccagcactct atctttccaa
     3661 atccacagca tagcaaagag agcaaaagag cctgtaaatt cagaaattaa ggaaacgcct
     3721 cttggtgtac ataatctatc ttatgttaaa tgaaatctaa tttcttgatt atatgctgga
     3781 tcgttcgtgg gtggctttgc ccctatattt ccagacacct gttatcattg gcccgctgcc
     3841 gcagatattc ccgtggcgag cgataatcca gcgcactatg cggatgccat tcgttataat
     3901 gctcgaatgc ctctgcaagg ttctttgctg ccgttaaccc gtcaggtttg ggcatgatac
     3961 tgatgtagtc acactttatt gttttcacga agctctttgc tattctgtta ctctccggac
     4021 tccgcaccgc cgtgttcttc ggttcaacaa agtcccaaca tccgggtgaa ctggcgtgtt
     4081 tcattagctc ggtagcatga accattatcc gtcagtcact tcactggaga cgccggaagc
     4141 tcgttgccga agcggtgttc caccgctccc agcatgtcgc caacgggaga gcatctacgg
     4201 tctgattgaa ctgtttattc aaattgtcca ccgtctgtca gtacgctctg aacgtcggct
     4261 ggttaaaaca ctgctcgctg attttcagag ggttcacggt aaaacagcac ttttattccg
     4321 gattgcggaa gcggctctga ataatccgga tgggctggtt aaagaggttg tgtaccatct
     4381 gcatattgtt gaaccagatc gcagcggtaa gagaagttca tcatacttgg cgcagttacg
     4441 cgatgtttct gctagaggtg atgccgtaaa gaacggcaga acattaccgg aacaagatag
     4501 cggtctacct gcactggtat cagacccggg gcttccccgg atgatctcca cggtacttta
     4561 ggtactgtgt ctatattatg taggtaggat caattaactt tgtagttgaa attctttcat
     4621 tgaaataatg aataaaaaag acaaaccgca ttaaacaatg ccagaatata attgcatttt
     4681 tgttccgttt ttttaaatca tagtatacaa agaagcttgc atactatatg aaaatataac
     4741 accctttact atgatgttta taaaacaata tatggagtca tgtaggtatg aatttagatg
     4801 gtgttagacc atactgtaga atagtcaata aaaagaatga aagcatatca gatattgcat
     4861 ttgcacatat aataaaaagg gtaaaaaatt catcatgtac tcacccaaaa gcagcattgg
     4921 tttttttagg agagaaaggt ttttgtgata gcaatgatgt tctatctatt atgggacaac
     4981 aaataccaag agtatttaag aacaagatgt tatatgatta tgtttttaaa aatgaaaaaa
     5041 gtaaaaatga ttttctaaaa atggctgaat catggctacc acagagtgaa ccaatagtaa
     5101 taaataatga tgatgacgca ttgaatgctg ctgcttattt ttctgtaaaa aaagcgaaaa
     5161 taaaaacagt aaacgatact gattttaaag agtataataa ggtttatatt cttgggcacg
     5221 gtagtcctgg ttctcatcaa ttaggccttg gttcggaact tattgatgta caaacaatca
     5281 tttcaagaat gaaagactgt ggtattctaa atgtgaaaga tatccgtttt acttcatgcg
     5341 gctccgctga taaagtggct cctaaaaatt ttaacaatgc ccctgctgaa agtctttctt
     5401 gtatccttaa ctctctgcct ttttttaagg aaaaagaatc tttgctagag cagataaaaa
     5461 aacaccttga aaacgatgag tcattgagtg atggtctaaa aatatccggc tatcatggat
     5521 atggagttca ctatggtcaa gagctttttc cctactcaca ttatcgttca acttcaattc
     5581 ctgctgatcc ggagcataca gtaaaaagaa gctctcagaa aaagactttt attattaata
     5641 aagaactgga ttagtataaa atttttaacc tatagttaat tgtttgcatt taagatgaag
     5701 ttattttatc tttagtagaa aagtgcttcc ctatgaggga aaatcacaaa tcatcataat
     5761 acagagacaa aacgatacga aaaaatagat aaaaaacatc attcttttac cacattattt
     5821 tccgtgaata tgaaaaataa tgttattact ttaatataag actatttttt gtttttccat
     5881 cactctgttc aaatttttcc gcatgacttg tgttttttgt aatacagctc gttttttaca
     5941 gctgaccaaa atcatcaatt aattatgcta aggaaataaa ttatgaaaac caaaaacttt
     6001 cttctttttt gtattgctac aaatatgatt tttatcccct cagcaaatgc tctgaaggca
     6061 gaaggttttc tcactcaaca aacttcacca gacagtttgt caatacttcc gccgcctccg
     6121 gcagagaatt cagtagtatt tcaggctgac aaagctcatt atgaattcgg ccgctcgctc
     6181 cgggatgcta atcgtgtacg tctcgctagc gaagatgcat actacgagaa ttttggtctt
     6241 gcattttcag atgcttatgg catggatatt tcaagggaaa ataccccaat cttatatcag
     6301 ttgttaacac aagtactaca ggatagccat gattacgccg tgcgtaacgc caaagaatat
     6361 tataaaagag ttcgtccatt cgttatttat aaagacgcaa cctgtacacc tgataaagat
     6421 gagaaaatgg ctatcactgg ctcttatccc tctggtcatg catcctttgg ttgggcagta
     6481 gcactgatac ttgcggagat taatcctcaa cgtaaagcgg aaatacttcg acgtggatat
     6541 gagtttggag aaagtcgggt catctgcggt gcgcattggc aaagcgatgt agaggctggg
     6601 cgtttaatgg gagcatcggt tgttgcagta cttcataata cacctgaatt taccaaaagc
     6661 cttagcgaag ccaaaaaaga gtttgaagaa ttaaatactc ctaccaatga actgacccca
     6721 taaagctgga cagcctgtat caggctatgg agggcccata gacaaatcta ccctatatga
     6781 gcataggagg agtctatggg cacaccacgt tttacccctg aatttaaggg attactggaa
     6841 aggctgggac atatccctcc ggcagaagca gaaaaagctt attatgctgc catcggaaac
     6901 gatgatctgg caacctgagt tcacagataa aacattctct aggaaactcg gggcggttcc
     6961 gttcaccaca tgcaatgtgg tgttgcaggg gaacggtctg caccatcccc tatgtcgatc
     7021 aatataacag aaatgacaac ttcagattca gggcacaacc taaatatatt ttaggtcacc
     7081 tctcaaatcg tttgcctgat accgctccat tttttaataa aaaaatcaat catttttgaa
     7141 tctttgcttt tcattgcatt gtcaagcata gtgtccccac tattt
//
LOCUS       DAJTSF010000158         7183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000158 DAJTSF010000000
VERSION     DAJTSF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.159"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(296..1198)
                     /locus_tag="OO335_003777"
     CDS             complement(296..1198)
                     /locus_tag="OO335_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR6003984.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO335_003778"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO335_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR6003985.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="OO335_003779"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="OO335_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR6003986.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="OO335_003780"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="OO335_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR6003987.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="OO335_003781"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="OO335_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR6003988.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="OO335_003782"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="OO335_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="OO335_003783"
     CDS             7090..>7183
                     /locus_tag="OO335_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCR6003989.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1676 a         1812 c         1876 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAJTSF010000159         6910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000159 DAJTSF010000000
VERSION     DAJTSF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.160"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..694
                     /locus_tag="OO335_003784"
     CDS             <1..694
                     /locus_tag="OO335_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6003990.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1033..1803)
                     /locus_tag="OO335_003785"
     CDS             complement(1033..1803)
                     /locus_tag="OO335_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003991.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2282)
                     /locus_tag="OO335_003786"
                     /pseudo
     CDS             complement(1848..2282)
                     /locus_tag="OO335_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2294..2515)
                     /locus_tag="OO335_003787"
     CDS             complement(2294..2515)
                     /locus_tag="OO335_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003992.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="OO335_003788"
     CDS             complement(2516..3199)
                     /locus_tag="OO335_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6003993.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="OO335_003789"
     CDS             complement(3263..3574)
                     /locus_tag="OO335_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6003994.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3571..4473)
                     /locus_tag="OO335_003790"
     CDS             complement(3571..4473)
                     /locus_tag="OO335_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR6003995.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5872
                     /locus_tag="OO335_003791"
     CDS             4913..5872
                     /locus_tag="OO335_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR6003996.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5872..6267
                     /locus_tag="OO335_003792"
     CDS             5872..6267
                     /locus_tag="OO335_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR6003997.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6450..6830
                     /locus_tag="OO335_003793"
     CDS             6450..6830
                     /locus_tag="OO335_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR6003998.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAEPGCVP"
BASE COUNT         1888 a         1787 c         1675 g         1560 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggctcac caggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgtttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgac
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggcttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaaatac acctatgatt tgggaagcac
     5101 tgctgtcata ggtacaacac atgtcagtta tcaatacagc accaccaatc tgcttgccat
     5161 acatcatgca ctgctgacat ccggccttca gccacaggat gtagaactga cagtaacatt
     5221 acctgtgacc gaattctttg ataatgacaa tcaaccaaat gaagaaagaa tagaaagaaa
     5281 aaaggcaaat gttctccgtg agatatctct gaataaaggg gaaacattta aaattaaaaa
     5341 agttaatgta atgcctgaat ctttgccagc tgcatttgaa tcactaaaaa aagataaagt
     5401 aaacaaactt gaacgcagcc tgattatcga tcttggagga accactctgg attgtggttt
     5461 aattctcggt gcatttgaag gcatttctga aatccgtggc tattcagaaa tcgggacatc
     5521 ccgcatcact cataccgtca tgaatgcgct gacgaaagcc tccacaccat gtaattattt
     5581 tattgctgat gagttaataa aaaacagaca cgataatgaa tatctccaga cattaataaa
     5641 tgatgtagct gaaataaaaa acatttctca tgtaatagac agagaagtga aatctctggc
     5701 agaaagcatc aggcaggaaa tttcaacatt ctcaggaatg aaccgaatct atctcactgg
     5761 cggaggagcc gagcttattt accctcacat aaagcaatat ttcccgaatc tgaaagtgaa
     5821 caaggtggat gaacctcagt ttgcattggt gaaagcaatg gtgcatgcgt aatggaaagc
     5881 agtgatccaa aaaaaagaaa gaaagttgtg gcatacctgc acccggcact ttatccgcag
     5941 gacaacctga cacaacaaac aatagactct ctcccagtac aaatgcgggg agatttctac
     6001 agacaatccc ttatttgcgg agcagctctt tattctgttg ccccccgact gttaacgcta
     6061 atcagcgttt ttttcagtga gaaaatcact gcagaaaatc tggtgaaact tatagaacaa
     6121 acaacaggat acacatccac atcaattgat ataagtgtgt taaaaaacat catagaggca
     6181 tcttcagaaa acaaatcaga gagcatcact tcaaaagatg atttcgaaga acaaaccaga
     6241 cgcaatcttt ctatgttaaa aaaataataa aagaagagca ggttcagtca attacctgct
     6301 cttcttttat acatactaaa aaatcagatt tttacgaata ttcagattat cataaaaaat
     6361 gcaacagaca ggaatgcaat aattttgcat tatctcgttg cttgtaatgc aaatatgcat
     6421 tattgtcagt gtgtgacaat aacagagaga tgacgatggc gaccattaac gcacgtattg
     6481 atgatgatat aaagaaccaa gcagatgaag tgctgaagct gatgaatatc agtcaaactc
     6541 aggctattgc agctttttat cagtatatta ctgaacaaaa gaaactgcca tttgtgatca
     6601 cctcaattgt aaaaacgccg catgatttat tacgtgaaag tacagacatg ttagctgaag
     6661 ctcttgcagt gatttccaat ctacaggtgt ggactgaaca gcaggatggg attggaaaag
     6721 caaagctaat ggagtattac cgccgcctcg acgcactgta ctgttgtgca aaagagaaaa
     6781 taggtcttct ttcagataac cgggatgctg aaccaggctg tgtcccttaa tcatctgagt
     6841 tatagtaact gtctgtttac acaacatatt gaactatggc tcgctacgac cttcccgatg
     6901 aggcatggac
//
LOCUS       DAJTSF010000160         6832 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000160 DAJTSF010000000
VERSION     DAJTSF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.161"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..104)
                     /locus_tag="OO335_003794"
     CDS             complement(<1..104)
                     /locus_tag="OO335_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR6003999.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="OO335_003795"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="OO335_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR6004000.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            1354..2004
                     /gene="csgD"
                     /locus_tag="OO335_003796"
     CDS             1354..2004
                     /gene="csgD"
                     /locus_tag="OO335_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6004001.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2009..2398
                     /gene="csgE"
                     /locus_tag="OO335_003797"
     CDS             2009..2398
                     /gene="csgE"
                     /locus_tag="OO335_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR6004002.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2423..2839
                     /gene="csgF"
                     /locus_tag="OO335_003798"
     CDS             2423..2839
                     /gene="csgF"
                     /locus_tag="OO335_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR6004003.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2866..3572
                     /locus_tag="OO335_003799"
                     /pseudo
     CDS             2866..3572
                     /locus_tag="OO335_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3705..4196)
                     /locus_tag="OO335_003800"
     CDS             complement(3705..4196)
                     /locus_tag="OO335_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6004004.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4298..4852)
                     /gene="ycdY"
                     /locus_tag="OO335_003801"
     CDS             complement(4298..4852)
                     /gene="ycdY"
                     /locus_tag="OO335_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6004005.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4876..5613)
                     /gene="ycdX"
                     /locus_tag="OO335_003802"
     CDS             complement(4876..5613)
                     /gene="ycdX"
                     /locus_tag="OO335_003802"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6004006.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5668..6606)
                     /gene="ghrA"
                     /locus_tag="OO335_003803"
                     /pseudo
     CDS             complement(5668..6606)
                     /gene="ghrA"
                     /locus_tag="OO335_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
BASE COUNT         1890 a         1645 c         1479 g         1818 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaactgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcctg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tattttttat
     1021 atgtattatt agtaagttag caccatttgt atgatttttt aaaattgtgc aataaaaacc
     1081 aaatgtacaa cttttctatc atttctaaac ttaataaaac cttaatgtta acattttaat
     1141 ataatgagtt acattttgtt acatgtttaa cacttgattt aagatttgta atggctagat
     1201 tgaaatcaga tgtaatccat tagttttata ttttacccat ttagggctga tttattacta
     1261 cacacagcag tgcaacatct gtcagtactt ctggtgcttc tattttagag gcagctgtca
     1321 ggtgtgcgat caataaaaaa gtggggtttc atcatgttta atgaagtcca tagtattcat
     1381 ggtcatacat tattgttgat cactaaacct tctttgcagg cgacagctct cttgcagcac
     1441 cttaaacaat cgctagcaat tacaggaaaa ttacataata ttcaacgttc tctggacgat
     1501 atctcttcag gctctattat tcttctggat atgatggaag cggataaaaa gcttatccat
     1561 tattggcagg atactttgag caggaaaaac aacaatatca aaatattgtt gctaaatacg
     1621 cctgaagatt acccgtaccg cgacattgaa aactggcctc atatcaacgg cgttttttat
     1681 gccatggagg atcaagaacg tgttgtcaat gggttgcaag gcgtcctgcg tggcgaatgc
     1741 tactttacgc aaaagcttgc cagttacctg attacacatt ctggtaacta tcgttataac
     1801 agcacggaat cagccctcct tactcatcgg gaaaaagaga tcctgaataa gctgcgtatc
     1861 ggcgcgtcta ataacgagat cgctcgttcg ttgttcatca gcgaaaatac ggttaaaacg
     1921 catctttata atcttttcaa gaagatagcc gtaaaaaacc ggacacaagc ggtttcctgg
     1981 gcaaacgata acctcaggcg ataaagccat gaaacgttat ttacgctgga ttgtggcggc
     2041 agaatttctg ttcgccgcag ggaatcttca cgccgttgag gtagaagtcc cgggattgtt
     2101 aactgaccat actgtttcat ctattggcca tgatttttac cgagccttta gtgataaatg
     2161 ggaaagtgac tatacgggta acttaacgat taatgaaagg cccagtgcac gatggggaag
     2221 ctggatcaca ataacggtca atcaggatgt tattttccag acttttttat tcccgttgaa
     2281 aagagactcc gagaaaactg tcgtctttgc actgattcaa actgaagaag cactaaatcg
     2341 tcgccagata aatcaggcgt tattaagtac ggacgatttg gcgcatgatg aattctaaat
     2401 aaaaaattgt tcggaggctg caatgcgtgt caaacatgca gtagttctac tcatgcttat
     2461 ttcgccatta agttgggctg gaaccatgac tttccagttc cgtaatccaa actttggtgg
     2521 taacccaaat aatggcgctt ttttattaaa tagcgctcag gcccaaaact cttataaaga
     2581 tccgagctat aacgatgact ttggtattga aacaccctca gcgttagata actttactca
     2641 ggccatccag tcacaaattt taggtgggct actgtcgaat attaataccg gtaaaccggg
     2701 ccgcatggtg accaacgatt atattgttga tattgctaac cgcgatggtc aattgcagtt
     2761 gaacgtgaca gatcgtaaaa ccggacaaac ctcgaccatc caggtttcgg gtttacaaaa
     2821 taactcaacc gatttttaag ccccagcttc ataaggaaaa taatcatgca gcgcttattt
     2881 cttttggttg ccgtcatgtt actgagcgga tgcttaaccg ccccgcctaa agaagccgcc
     2941 agaccgacat taatgcctcg tgctcagagc tacaaagatt tgacccatct gccagcgccg
     3001 acgggtaaga tctttgtttc ggtatacaac attcaggacg aaaccgggca atttaaaccc
     3061 tacccggcaa gtaacttctc cactgctgtt ccgcaaagcg ccagggctta caaaacctgc
     3121 ttaacgagcg caaggttatt cgtgcggcac aagaaaacgg cacggttgcc attaataacc
     3181 gaatcccgct gcaatcttta acggcggcaa atattatggt tgaaggttcg attatcggtt
     3241 atgaaagcaa cgttaaatcg ggcggtgttg gggcaagata ttttgggatc ggtgcagaca
     3301 cgcaatacca gctcgatcag attgccgtga acctgcgtgt cgtcaatgtg agtaccggtg
     3361 agatcctctc ctcggtgaac accagtaaga cgatactttc ctatgaagtt caggccgggg
     3421 ttttccgctt tattgactac cagcgcttgc tggaagggga agtgggttac acctcaaacg
     3481 aacccgttat gctgtgtctg atgtcggcta tcgaaacagg ggtcattttc ctgattaatg
     3541 atggtatcga ccgtggtctg tgggatttgt aaaataaagc agaacggcag aatgacattc
     3601 tggtgaaata ccgccatatg tcggttccac cggaatcctg acagataaaa atgccggaat
     3661 taataatccg gcatttactg tttaagcgaa agcgtgagtg cccctcacgc tttatttttg
     3721 atttcctgct cacggtgagc cgtctttgca ctacgcgccg ccagccacag gccgctgttt
     3781 ttcattgcgt aaccaaatac cagcccaagc gccagcgaag gtaacaccag tttccagtca
     3841 ccctgcccgg caaacgtcgc gcatgcgcct ataaacgtcc ccggaacaaa tgaaagcagc
     3901 agctgtttgg cctgaataca catcagaaac gcgacaatac cggttatgac ataaccgaga
     3961 atttccagat gtggtgccag tgcactaccg taaataatga ccatcgccca caccacgcca
     4021 ctaagcaggg ttgcagcgga gatcgccagc cctttcagcc caccttgcgg gcaagcaaag
     4081 taggccgtac agcccaggaa gcccgcccag cttagtaagc ccagggaaac agccacccat
     4141 ccccagatac cggagagaat gcctgttgtg attgcaatag agagaagtat gttcatgccg
     4201 cgcatgatag cagaaaacgc ggccatgaat gagatcgagc acacatttta tgcaacaaca
     4261 aattatctgt tgcagttatg agtgacgatg atcacattta ctcttcagaa tcttcttcca
     4321 gctcgtccca cattgcacta atggcatcac gggttagcgg tgccatggtg cgccagaaag
     4381 gcgtggttgc atgggcctcc actttgccga ggaacgcacc acaccagggt aacagatact
     4441 cactgaacag tgtttccagt gcttcgctct catcttccgt tgactgatct tccagccagg
     4501 aagccgcaag cagcaatgtg ccgatgtgat cggctggcgt atccgccagt agcatccctc
     4561 gttcggaaag aaaagcgcgc acttccgctt ccgttgcgtc ctcaacccat gcgctacgat
     4621 atggcggcac ggcacattca tcgccgataa acaacgcatt gtaatcggca gagacttgcg
     4681 tcatatcaca gcttttctgt aaacgcgtca gtaactcatc ctgctccagt ggccagttcg
     4741 cagccagttt cccctcacga atcagagtaa acagcggcac cagtaaagga tcttgcggtt
     4801 ggcggtaata gagcgaaccc agcacacgac agaggataga aaactcgttc attaatatat
     4861 ttccattact agtaattaaa ggtctgcaaa ttccgcaatc ggtgccatac cgcgagattc
     4921 aaggaagttc agtaagcggc gcggagaaac attcaaaatg cgctctagcg gaaaatctac
     4981 cgcgtcgagg attttaagac actcttcaaa ttcccccatg gtaaacgcgg tgtgagaatc
     5041 cgagcctaat gccacccaac ctccagcatc acgtaccgct gcagctaccg cacgacagtt
     5101 gtcttcactg cccttacgtg agtgtaaaaa tgaggaatta ttgatttcca gcgccacctg
     5161 atgtttcgcg gctgcttcag caacggcttt aacatctatt tcatatttcg gatttccggg
     5221 atggcttatt atatgcacat tgccgctggc gatagtggag atcatcgctt gtgtattggt
     5281 cgctttgtca tgtggcgcaa aaaccggctc atgaaaaccg gcaataatca gatccagcga
     5341 gtcaaacatt ttaccgctgc agtcaatttc accatcaaca tttttaatgt tagcttcaat
     5401 gccgcgcagg atccctaccc catcaaccac tcgcggccag atacgcatgt taatgaagtg
     5461 ccagtgatgc ggcgcatctt ccatatccgg gccatgatcg gtgatcgcaa aaagtttaat
     5521 gcccttttgt ttggcctggg caatgtaatc actcaatgtg ctatatgcat gtgtgctggc
     5581 aacggtatgc atatgaaggt cgacgggata cataactctc tcctgtattc aattcccgcc
     5641 aaggatagca ggaatcctga tgctttatta gtagccacgt gcgcggtcga cttgcccgca
     5701 gaccctctcc cctttttcga gctgggcaat agtgcgagaa atgtactcca cagcttcagc
     5761 gggacgggta atcgcggcga catgtggtgt tatcgtcacg cgtggatgtt gccagagcgg
     5821 actttcaggc ggtaagggtt cacgattaaa aacatccagc attgcgcctt taactttccc
     5881 gctatccagc gccgcgagca ggtcatcttc cacaacatga acaccacgcg ccaggttgag
     5941 gagatacgcg ccatcaggta atttttcgag taattgttga ttaataatgc cgacggtttc
     6001 aggggtattc ggtaacaaat taatcaatac ccgacattgg ctcagaaatg cagacagttc
     6061 ttcccgtccg gcaaagcttt gcacgccagg ccacgattta cgggttcgac tccagcaacg
     6121 cagcggaaag cgctaggttt gcagactctg agcaacttta cagcccagta cgcctgcgcc
     6181 caaaatgccg atggtaaaat cttcccgatg atattcaggc agcggttgcc aatgcgaact
     6241 attttgctgg atgcgataat cgtcaaaacg tcgaaaccaa tgcagcacct gactgacagc
     6301 atattcctgc atttgctcgc ccataccggt atcttccagg cgaaaaagtg gaacagaagg
     6361 gttcagcatt tcagggtgtg cctgtagctt gctcaaaata gaatcaacac cggccccgag
     6421 tgcgaacacc gctttaagat cgcgccctgc cagcatttca acaggaggat gccagactaa
     6481 aacataatca gcagagtcat tatctccgct tttccatgcc ctgactcttg cctgaggaat
     6541 agctttgcgc agtgcctcaa tccaccattg ggtatcgaac gttgggtgat aaaagatgat
     6601 atccatactg actcccgaaa agcgttgtgc gaaatttatt cgcacttatc gttatgatct
     6661 acaaaggcca ccagcataac aaatccgtgg tcggtggcaa aaaaagcaga tttcgcttat
     6721 taaaaccaca cattgattga atgtttgaat aaacgcgcga ttttttcaaa aagtttgttg
     6781 acctcaggtc atgatttccc taaattagcg cccgttccag caagacagga ac
//
LOCUS       DAJTSF010000161         6795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000161 DAJTSF010000000
VERSION     DAJTSF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.162"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            240..2672
                     /locus_tag="OO335_003804"
     CDS             240..2672
                     /locus_tag="OO335_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR6004007.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="OO335_003805"
     CDS             2739..4208
                     /locus_tag="OO335_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6004008.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="OO335_003806"
     CDS             4208..5869
                     /locus_tag="OO335_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR6004009.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..6431
                     /gene="symE"
                     /locus_tag="OO335_003807"
     CDS             6090..6431
                     /gene="symE"
                     /locus_tag="OO335_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR6004010.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6478..>6795)
                     /locus_tag="OO335_003808"
     CDS             complement(6478..>6795)
                     /locus_tag="OO335_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004011.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         2003 a         1612 c         1554 g         1626 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
     6361 tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga
     6421 aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta
     6481 taccaacgcg ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg
     6541 aagttgtgcg aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt
     6601 tgtcacatct ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga
     6661 caatcgcagt cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc
     6721 aatatatagc ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg
     6781 aagatgatcg aataa
//
LOCUS       DAJTSF010000162         6559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000162 DAJTSF010000000
VERSION     DAJTSF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.163"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            154..504
                     /locus_tag="OO335_003809"
     CDS             154..504
                     /locus_tag="OO335_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR6004012.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="OO335_003810"
     CDS             557..952
                     /locus_tag="OO335_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6004013.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="OO335_003811"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="OO335_003811"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6004014.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="OO335_003812"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="OO335_003812"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6004015.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OO335_003813"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OO335_003813"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6004016.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="OO335_003814"
     CDS             complement(5323..6423)
                     /locus_tag="OO335_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6004017.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
BASE COUNT         1505 a         1716 c         1554 g         1784 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcat
//
LOCUS       DAJTSF010000163         6520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000163 DAJTSF010000000
VERSION     DAJTSF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.164"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            439..1827
                     /gene="narU"
                     /locus_tag="OO335_003815"
     CDS             439..1827
                     /gene="narU"
                     /locus_tag="OO335_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR6004018.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1909..5649
                     /gene="narZ"
                     /locus_tag="OO335_003816"
                     /pseudo
     CDS             1909..5649
                     /gene="narZ"
                     /locus_tag="OO335_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5646..>6520
                     /gene="narH"
                     /locus_tag="OO335_003817"
     CDS             5646..>6520
                     /gene="narH"
                     /locus_tag="OO335_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6004019.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1558 a         1713 c         1690 g         1559 t
ORIGIN      
        1 cttagatgaa ataaacaata cttactataa taacgattat tacaatcagg caataagaga
       61 ggaaccgata gactttctgg acagatcgtt ttcctccaat tcatggcctt tttagtgaat
      121 aaatcatcac taattctata agtgtctggt cttgttgaga atatagttaa tatgtaaccc
      181 tttagattca cgatctgaag ggttgaaaca tgacatacgt tcaaagatta acccccgttt
      241 gaaggttagc ttgttagtta catttagtaa cacatatctg gctacttaat ccccattctg
      301 cccccgcggt tacgctttct tatctattct ttcaatcagc acttcaccca attcatcctt
      361 aattctaata actggctatt gcgccttacc tcctgttttg tcttttttca ttaccaatgt
      421 gtgcatatga ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc
      481 gactggaaac cagaaaatcc ggccttctgg gaaaataaag gaaaacatat tgctcgaaga
      541 aacctctgga tatcagtcag ttgtctactt cttgccttct gtgtctggat gctatttagc
      601 gcagttaccg ttaatctcaa taaaatcggt tttaatttta ctaccgatca actcttttta
      661 ttaaccgcat taccctccgt ttctggcgca ttattgcgtg ttccctactc ctttatggtg
      721 cctatattcg gtggacgccg atggccggtt tttagtactg caatcctgat tattccttgc
      781 gtctggctcg gaattgccgt gcaaaatccg aatactcctt ttgggatatt tatcgttatc
      841 gctttgctat gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc
      901 ttctttccaa aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta
      961 ggtgtaagtg taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt
     1021 ctcggcgtca atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca
     1081 tggatttggg taccgctact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat
     1141 atcgccagtt cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc
     1201 tggctgctga gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt
     1261 tttgccatgc tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt
     1321 ggcccattta ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc
     1381 ggcgtgcggg tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc
     1441 cttaccttac cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg
     1501 ctgtttctga ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt
     1561 cgccagataa ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga
     1621 gaagccgtca ccgaaacggc ggcaacgctg ggctttatct cagccattgg cgcagtgggc
     1681 ggctttttta ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc
     1741 gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat
     1801 ggtcggcgga agttcagcca aaaataagag atcgatgcct gatgtacaac catgtcgcat
     1861 ccgacaaggc atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt
     1921 ttggatcgct ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag
     1981 gtgatgcata gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa
     2041 atcgtgcgtt ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt
     2101 aaaaatggtc tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac
     2161 ctgcccaatc atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac
     2221 agcgctaacc gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa
     2281 gccctcaaac aacacagcga tccggtactg gcgtgggcat cgattatgaa tgatccgcaa
     2341 aagtgcctga gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag
     2401 gaactaaacc agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat
     2461 cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaacg
     2521 cgttatctgt cgctgcttgg cggcacctgt taaagtttct acgactggta ttgcgacctg
     2581 ccgcccgcct cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg
     2641 tataactcca gctacatcat cgcttggggg tctaacgtac cgcagacacg tacgccggac
     2701 gcccacttct ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac
     2761 tactctgaag tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc
     2821 gccctggcga tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc
     2881 gactacttta tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag
     2941 cctcgcgacg atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat
     3001 ggactgggcg aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa
     3061 ttggtagtgc cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg
     3121 gaatccattg ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac
     3181 gctgttgcag gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc
     3241 gtaaaacaca atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat
     3301 ggcaacacct gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat
     3361 cgcgggctgg aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca
     3421 gcctggggtg agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa
     3481 tttgccgata ccgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt
     3541 aaccactggt atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc
     3601 ggttgtgtcg ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc
     3661 ccacaaaccg gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag
     3721 atgaacagca cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct
     3781 gctcaggagt tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat
     3841 ttcaacgttc gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac
     3901 ccgctcggga ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc
     3961 caggcgctga aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat
     4021 catccgcgta atttgtttgt ctggcgctct aacctgcttg actcctccgg caaaggccac
     4081 gagtatatac agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt
     4141 gccagcgacg ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag
     4201 ctcgacctgc tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc
     4261 gttctgccca ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca
     4321 tttattcatc cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa
     4381 atctacaaag gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa
     4441 accgacgtgg tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt
     4501 gaagtgctcg actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat
     4561 attgtggcgg tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca
     4621 ttgatggaca aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc
     4681 gatttcctcg gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg
     4741 attgacaccg ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt
     4801 catgttgcag tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat
     4861 ctggcgctgc acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt
     4921 aaaattatct ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg
     4981 ggatacacca acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc
     5041 tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct
     5101 atcgacaccc gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa
     5161 gcacttaact tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac
     5221 ctgctaatgc tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc
     5281 cgagaactga ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg
     5341 actgcccgcg cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc
     5401 caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat
     5461 aactcggtca cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg
     5521 gcctggggct ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc
     5581 cgcaagatga agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg
     5641 aaaaaatgaa aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct
     5701 gccatacctg ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg
     5761 catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc
     5821 aggaagagtg gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg
     5881 gcagcaagat gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg
     5941 atgattacta cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca
     6001 aacatatccc tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga
     6061 tctggggccc aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc
     6121 gcaacttcga ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt
     6181 acctgccgcg cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg
     6241 gcgctatcta caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg
     6301 gctggcgttt gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg
     6361 gcaagtcaga aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt
     6421 gctcagaaac ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc
     6481 gcatcgagga agcgtcgagc accgagcgcg aagttgacct
//
LOCUS       DAJTSF010000164         6200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000164 DAJTSF010000000
VERSION     DAJTSF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.165"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO335_003818"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO335_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6004020.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO335_003819"
     CDS             2089..2238
                     /locus_tag="OO335_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004021.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2983..3423)
                     /locus_tag="OO335_003820"
     CDS             complement(2983..3423)
                     /locus_tag="OO335_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6004022.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3641..4030
                     /locus_tag="OO335_003821"
     CDS             3641..4030
                     /locus_tag="OO335_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004023.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4074..4478
                     /locus_tag="OO335_003822"
                     /pseudo
     CDS             4074..4478
                     /locus_tag="OO335_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4585..5421)
                     /locus_tag="OO335_003823"
     CDS             complement(4585..5421)
                     /locus_tag="OO335_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR6004024.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVSLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5421..>6200)
                     /locus_tag="OO335_003824"
                     /note="aph(3'')-Ib"
     CDS             complement(5421..>6200)
                     /locus_tag="OO335_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR6004025.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1433 a         1616 c         1640 g         1511 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgttgcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcat tccttcagct catacgtcac tttcatcctc
     2641 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
     2701 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     2761 gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc tatgcacttg
     2821 tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2881 ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt
     2941 gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg aaccggagcg
     3001 aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct
     3061 aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg cttcccgcgc
     3121 catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat
     3181 gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc cctccttgtg
     3241 cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt cactggtctc
     3301 gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag ccatgtcacg
     3361 atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc
     3421 cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt
     3481 ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga
     3541 cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat tgttataaat
     3601 acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag agttaaatag
     3661 cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg atggtcatag
     3721 cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc atgataatag
     3781 tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc cgataactgt
     3841 cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata acattataca
     3901 tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa gagtgggagt
     3961 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
     4021 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
     4081 atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac
     4141 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
     4201 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
     4261 aatgcgggcg gctggagcat ggctttctac gggttcgctg cgagtcttgc cacgccgagc
     4321 acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga
     4381 tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt
     4441 gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc
     4501 ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat gtgttcccag
     4561 gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct tgatcgcggc
     4621 cgcgatagct agatcgcgtt gctcctcttc tccatccgca ttccaagctg cggaaaggca
     4681 cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg cacgagagaa
     4741 tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc tgtcagccgg
     4801 atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagactga cgggatctat
     4861 caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc catgtagccc
     4921 acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt ggacgtagtc
     4981 agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg caaagcgatc
     5041 ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca gcttcgccat
     5101 tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga tgtgagagag
     5161 cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga gcaaccggac
     5221 tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat cagcaatgtc
     5281 ttctataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg aaactttcca
     5341 aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc aatgagcagg
     5401 aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag atagaaggca
     5461 aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc gaaggcgcgc
     5521 tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag tgccaaatcg
     5581 gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca ttgaagagtt
     5641 ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac catatcggtg
     5701 cgctcttggt cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc
     5761 ggcgtactct tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca
     5821 tcaacggcgc gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat
     5881 aggctgtgaa cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct
     5941 ccagacagat cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc
     6001 tgttcctcct gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg
     6061 aggcggtcac gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag
     6121 gcatgcccgt caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac
     6181 cagtcagaat gcgattcacc
//
LOCUS       DAJTSF010000165         6161 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000165 DAJTSF010000000
VERSION     DAJTSF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.166"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..171
                     /locus_tag="OO335_003825"
     CDS             <1..171
                     /locus_tag="OO335_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6004026.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="OO335_003826"
                     /gene_synonym="chbF"
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="OO335_003826"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6004027.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="OO335_003827"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="OO335_003827"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR6004028.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO335_003828"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO335_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR6004029.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="OO335_003829"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="OO335_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR6004030.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5430..6161
                     /locus_tag="OO335_003830"
                     /pseudo
     CDS             5430..6161
                     /locus_tag="OO335_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1579 a         1311 c         1558 g         1713 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt taattattta tttattttaa aatggttaag ttttgctaat catgatgagt
     5341 tatttttgat agttcatcct gtggcgtcgc tcttaaactt atattgtaaa tgacttagat
     5401 ttaaataaga taataatttt gttaacaata tgaggcggga gatgttttct acaaacttaa
     5461 tacagagtaa ttatggtgat ttaaatatta aaagcctggc tttcgactct tttaaagaaa
     5521 ggcttgagtc taccatggcg gcactcactt tctttatatc aacgggtcaa tgtgactgcg
     5581 atgaaatagc agagtcgaac ttcaatcgta tgattgccta tatgtctaac attaactatg
     5641 atgcctcaaa gctaggagcg ccagcactta gttttgatac gtatctgcag gacaatgtta
     5701 aatatagagt gataattaat aatctttatg gcagtgagtt tcggataaga gatattaatg
     5761 agaattttgt tgggatggat tttacctgtg aattccgacc cgaaaaaatg acgaatttga
     5821 tcaatataaa aaataggttg gttattcatt atttaaggac tatatactat gaaaaaaatt
     5881 atatacatcc tgccgggagc atttttgctg cgattcaaaa aaaatgagag tctcctaaag
     5941 tttccttcga taatatcaat gcttaatatc aatcttcttt ttaatccact aaatcttccg
     6001 ggaatgggaa gtggtgtgct tgaagatatt atgagtattc cagatagttc acttagaaaa
     6061 cgtctcgggt atgaggtttt aagcttctca ctacaggcac attcattgag ccaggaatgt
     6121 attgataagt tggacatttt tttcgctgac gatctgttta a
//
LOCUS       DAJTSF010000166         6136 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000166 DAJTSF010000000
VERSION     DAJTSF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.167"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO335_003831"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO335_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6004031.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO335_003832"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO335_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6004032.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO335_003833"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO335_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="OO335_003834"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="OO335_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1618 a         1442 c         1450 g         1626 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagata
//
LOCUS       DAJTSF010000167         6058 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000167 DAJTSF010000000
VERSION     DAJTSF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.168"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..143)
                     /locus_tag="OO335_003835"
     CDS             complement(<1..143)
                     /locus_tag="OO335_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR6004033.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="OO335_003836"
     CDS             379..651
                     /locus_tag="OO335_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6004034.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="OO335_003837"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="OO335_003837"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6004035.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1731
                     /locus_tag="OO335_003838"
     CDS             1111..1731
                     /locus_tag="OO335_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6004036.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="OO335_003839"
                     /pseudo
     CDS             complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="OO335_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3114..3767)
                     /locus_tag="OO335_003840"
     CDS             complement(3114..3767)
                     /locus_tag="OO335_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6004037.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3767..5238)
                     /locus_tag="OO335_003841"
                     /pseudo
     CDS             complement(3767..5238)
                     /locus_tag="OO335_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5211..6058)
                     /locus_tag="OO335_003842"
                     /pseudo
     CDS             complement(5211..6058)
                     /locus_tag="OO335_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1621 c         1599 g         1411 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggatc ttcggtgcgt tgtgctggat gacgaccttt
     1681 acgcgggtgt ggagtgggta tctgacgctg aagtcgctcc agcgtcagta attgcaatgt
     1741 tatttgctac tgcccgggcc gcgttcaccg gttgcaaccg gattttttgg atcgctgctc
     1801 cattcgtacc agccgccgtc atacacggaa acattcttcc aacccatggc gcgtgcgtac
     1861 ataaaggttt cggacgcgcg ccagccggtg ccgcagtaga atgaaacttg ctgatctggt
     1921 ttgatattcc atgctttcca catagcggta atatcatcgg cgctacgcat ggtgccatcc
     1981 gggttataga aatcttccat atgcgtcgag tcgctaccag cgtgtcccca acgtgctccg
     2041 gctatttcac cttttggttt aatatagctg taaccgctgg tcgtaccgat aaattctggc
     2101 cacgaacgaa tgctcaccag cgatgcatcc tggcgatgca gcagtccacg ggcctgttcc
     2161 atatcgagca tcaactgcgg ttgtgccggg atcttcacgc cgaaatccgg ttccgctttc
     2221 actttcggtg gcgttcctcg ctcaacaggc agtcccgcgt cggaccaggt ttgccagccg
     2281 ccatccagca gacgcacatc tttcacgcct gcgtagagca taatttgcgc cacacgcgct
     2341 ccagcgtata cgtcacgccc gtacagaatg accgtggtgt catggcgaat gccgtgtttt
     2401 gccagcatcg cttttagttg ttcatcagaa actttgttcc acagcggttc gctttccact
     2461 tcgttggtat cgatgtagtc agcgccagga atatggctga taaggtaaag cttaggagcg
     2521 ccctaggccg cttcaatgac tttccagtca ccgacaggtt tcgccgtaac ctctttacct
     2581 tgttgcaggt catgcagcca ttgcggataa accagctgct caaagtgcgg cagtttttgt
     2641 agacgggaag gttcgcttag cgcgtcactc agaatggaga tatgcgtaaa acctgctttt
     2701 tgcagtcgcg ttttgacggc gtcaacatct ttgccattac cgtacagcgc caccggggca
     2761 tcggctttca ggttatgttg cttgatccac gcgttgagct gttcggtgct cattttgtcc
     2821 agccagctag cagagaggtt taaggcggca ggttcatgac cagaagggcc atttaaggtt
     2881 tgtggccagc cgttataaaa cgcgctgggg cgagtatcta tcgctttgcc attttgttgc
     2941 tgaagttggt caagtgtaag aggcttcgcc agttcagcgg cccacgaaga agcgcaagcc
     3001 agccctaaag ccattgccag cgcggtcatt tgagaaacac gtttcatcga aaatcccgac
     3061 attgtagttt tgagtgcgcc attctgaact gacgcattga gaaaaacttt gcgttatcgc
     3121 agtttgttgt aattttctga ccactgcgcc atatccagaa cagaactatc agcaggaaca
     3181 tcctggagat cgtgcgttac ctgaacgacg gggatcgcca gggcgcgaac ttcgctgaac
     3241 acccactggc gaaaattatc gcgcagagcc acatcaagac ggctgaatgg ctcatccagg
     3301 agtaacgctt ttggctgggc gagaagggcg cgtagtagag caacgcgcgc tcgctgaccg
     3361 ccagacaaag tggcaggatc ctgatggaaa gctccgtcaa ggcctgaacg ctcaagtgca
     3421 tcattcacgg cattacgtcg ggcattcccc ttaagtgtcg ccggtagcgc cagcagtaaa
     3481 ttttgcccga cgctgaactg gtcaaataac agtgcatcct gaaaaagaat gccaatctga
     3541 cgctgtgcgg tgggtagcat gtcaatccgt tgctcattga gccatagctc acctgtacaa
     3601 gaaaactgtc cggccagtgc accaatcatc catgaaaaca gagtggattt tccacagcca
     3661 gacggcccca ttaacgtgac gatgtcacct ttatcaaccg taaagttaac gtttgtcagc
     3721 aagcggcttt ctggtaaacg tagcgaaaca tttttcacgc agagcattag cggattcctt
     3781 gtctgacata acctacccat tttgcgacta acgcggtcag ggcaaaaata ataagcggta
     3841 acaacagttg ccataacgcc tgggcggcga gaataccgtt gctgccgccg ctgcttaatg
     3901 ccaccgcctc agtggtgagc gtcggaaaac gccccgcgcc cagccacagc gtcggcatat
     3961 actgcgcaat actgactgaa aatcccaccg cgaaggcaat cagcacaggg cgcaacatga
     4021 gtgggcattt cacgtaaaag aagattttgg cccgcgacca gcccagtgtt tgcgcaatca
     4081 aaattaaccg tgaatcaatg cgctgccagg cgggttgcag gataaacagc atccacggca
     4141 ttacccacag cagatgcccc cagaccaccg ccgtccagct tccatccagg ttcagccata
     4201 gcgccagcgt gtactggccc gccaccagtg gcagagcggg caatagaatg ggcaaccata
     4261 gccacaactg gcgacgctgt gccccccatt ccagccacag cagtaacagg agcaaagcga
     4321 tgaatgttgc taccagcccc attgtcagac tgttgatgag tgcttcacta ttgatcgtcg
     4381 actgatccgc gaggatcgcc agcagaacca cacacagcac accggttaag ggtaaaaaac
     4441 tcgccagcgt agtgcctggt aataaaggtg tggcaggctt gcgaatgcca tctacgcggg
     4501 gaatagtgcg ccgccagctg cgccaaaacg taggcggcga gtaacagcat cagcagcaga
     4561 ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct gagttaacca ctgccagcta
     4621 attactgcca gcgtcggtgg attacctggc ccaagaataa tcgccacatc cacgaccgaa
     4681 agcgaccagg caacaatcgc cagcatcgcc attgccagcg gctgtagccc agcgaatcca
     4741 gcacaatgac ctgctgcaac agccgttttt cgctcaatac tgccactaag atccacagca
     4801 gaaatgcgct ttctttcact gcaagggtga ggcccagacc gatccaaatc ggtccattgg
     4861 cggagtgaaa tacgggaaat agtcatatag cagccctccg tcagcaaaga gcagcagggc
     4921 gctggtggca aaagccacat ggggaatggc gagcagccac ggcagacggg cgcacatacg
     4981 ctgccatttc ggcccaggcc atagcgccac aatcaccagc agggcaatca acaatgcccc
     5041 gaccgccgcg atggttgtcg acaccagcgt tgccagtaat gcctgcggta actgtggatc
     5101 ggcaaataac gcctgccagc gtgtcaacga caacgcaggc gatatcagcg tgagagctgc
     5161 cgggatcagc ggtgcataaa tcaccgccac catcgcccac agcaaaaaga ttaatgcgta
     5221 ccgtaacggc gtaaccattc ttgttccagt gcattcaccc atcctgcgtg cggttcagcc
     5281 agtaccggcg gcagatcctg cggcattctt gattgcaata tttcgcgctg cccgtcaggc
     5341 agtttttgcg gatcgagaac agaaggatcg ccccagacaa cgggatctgc tttacgcagt
     5401 tgcgcatcgg gtgagagcag gaaattggca actaccttcg ccgcagcact ggcattcgcg
     5461 ttggctggaa tggtgacgaa atgcacgttg ccaatcatcc cctcgcgaaa gccaaaactg
     5521 taactgcttg ccggcaaatc gccgctggcg attttttgct gcgcatgtgc ggggttaaag
     5581 gtcagcgaca ggcgcaatgt gccggctttc agcagagcat ccatccgcgc gggcgaaggc
     5641 gggaaatcct ttccttcgcg ccacaaatac ggatgcagtg catcaagata ttgctacaag
     5701 ggcgcagtga cacgggcgaa agtcgcatcg tccggcgctt cttttaatgc ggcgggatct
     5761 ggcgtcagca taatcagcaa ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc
     5821 ggataagtaa ccgtgccggg attggcttta gcaaactcca gtaaggcttg cggcgtttgt
     5881 ggtggctgtg gcgtaacatc gcggcgggcg ataaacgtca gttgtgcgcc gccccagggc
     5941 gattccgcac cttgtgtcgg cactgaaaaa tctccccgca ccggcagctg tgtgtcgaca
     6001 tagcgccagt tgggcagagt ctccgcccag cccgtttgta ataaattggc ctctttta
//
LOCUS       DAJTSF010000168         6052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000168 DAJTSF010000000
VERSION     DAJTSF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.169"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            430..1338
                     /locus_tag="OO335_003843"
     CDS             430..1338
                     /locus_tag="OO335_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR6004038.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="OO335_003844"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="OO335_003844"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6004039.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /gene="loiP"
                     /locus_tag="OO335_003845"
                     /gene_synonym="yggG"
     CDS             3653..4411
                     /gene="loiP"
                     /locus_tag="OO335_003845"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6004040.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="OO335_003846"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="OO335_003846"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6004041.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>6052)
                     /locus_tag="OO335_003847"
     CDS             complement(5673..>6052)
                     /locus_tag="OO335_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004042.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1491 a         1576 c         1561 g         1424 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagcaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccggatggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgccaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 tcgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgttta
     5881 tctttgcgga atcctttaat cactaacgaa tctccggcat tcacaatata gccgttgtta
     5941 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat cagccgtagc aacgatctcg
     6001 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc ga
//
LOCUS       DAJTSF010000169         5982 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000169 DAJTSF010000000
VERSION     DAJTSF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.170"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..392)
                     /locus_tag="OO335_003848"
                     /pseudo
     CDS             complement(1..392)
                     /locus_tag="OO335_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(578..3886)
                     /gene="icsA"
                     /locus_tag="OO335_003849"
                     /gene_synonym="virG"
     CDS             complement(578..3886)
                     /gene="icsA"
                     /locus_tag="OO335_003849"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR6004043.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4415..5617
                     /gene="virA"
                     /locus_tag="OO335_003850"
     CDS             4415..5617
                     /gene="virA"
                     /locus_tag="OO335_003850"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR6004044.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5796..>5982
                     /locus_tag="OO335_003851"
     CDS             5796..>5982
                     /locus_tag="OO335_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6004045.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1840 a         1355 c         1018 g         1769 t
ORIGIN      
        1 caggctgtac gcttccccat gtgatttgcc tcgccacttc accgggctca ggaccccgta
       61 ggatcttcac cgaaaaaaat gcatcaccag ttgctttatt atttgtttta ggtagacctg
      121 ttactaattt atacctactc tgaccaagtc tggataagaa aggcacacct gcacatttct
      181 acttggaaat gttttctttt accattttgc tcaaaaattt caacatccaa tattcctcgt
      241 acagaactac tcaagtcctg aatagaaaaa gttccagctg gtacagtaga gtcatatatt
      301 actcgacctt gttgagatac aacaacacgt gcattagttt ctgcaatacc agtaatctga
      361 ggcgcataac cacgaatatt tggcggtagc attcgttcat cgctttctaa tgcaattcct
      421 gtgtaacgcc aagcaacagc gcattctggc gcatgatctg gtaaacacgt ttggcattga
      481 tcgcaggcat accatcatgt gcacaaaaac ccaccgagct tttacacggt gggtcccaga
      541 gaaatgcagg acatcaacac gccctgcatt tttattatca gaaggtatat ttcacaccca
      601 aaataccttg ggtgtctctg taactgttat tacccaattg ctgagatacg tttccccaga
      661 tgcttaggtt atctgcaaga cgccccccca caccagtttt gagttcaatc acattacggt
      721 tgcctatctg gtgatttgac atgccattca taattacacc atattgcttt gagctccatt
      781 tccaattcac ttccaaaaat ggttcgaact tacgaagagt ctccttatcg atacaactct
      841 gactaattaa ggatgtacgc aatcccaatt ttgcttgaat attattgccc ccagaggatg
      901 tgaccatagt gccattgtgt tcaatatagt cgtttgcttt taccccatgc ctagttatct
      961 ggacttgtgg attgagatac aatgcgttgt caatattatt atgagcagtc cagcgtatgg
     1021 ttgggtagat atagccagct tccaaagctc ctgttattcc tttgctggaa tatttctcac
     1081 cagttaaccc atctcctttg actgtattat taaaccaact gtactgcaac caagcatcca
     1141 tatatagacc tgttcgttct ttttcatcct gaaaccaaga actgtacaaa ccggcagtat
     1201 aaccgctcac agttcctcgt gaactataac tagtatactt attatgagtt ttgctatcct
     1261 gatatccata agctcccata atacccacat gcaagccatc atgatcagtg aagtttgtct
     1321 tcaaaatatc tccaccaatc tgataaacca tcctgttagc tgtggttttt aattgtctat
     1381 cagctaaatt atgctcatta tgtccaccaa cagtacgaat ccacatggtg gtttcgctga
     1441 gctgacctgt agtaggatca acaatagtgc taccgtgtcg gtcatagaga gaaggactaa
     1501 acatcgtgtt cattgctatc aggttagcaa tataactgcc agcttcaggg cgccatgtgt
     1561 gaatactacc ttcacccaaa ttaagtgaag atacaagctg tgaactagct cggcgactac
     1621 tcatttgagt agactcttga ttgctcatct gttttgattc ttgattatcc atctgactag
     1681 ttagatacca cttattggta ttcagtccag atacagtgcc ctgtttcagg cgatagtcat
     1741 aactaccagc aacaatacgg cctttctgaa taaaagcatt cttatcagaa gaatctgtcg
     1801 aaataatttg aacaccttca agagttttat ctccagagcc tccctcattt gtcactgcaa
     1861 cataggtaat accagacgca tggcctgcaa tttccagccg gtcagtttta ctgttgtcat
     1921 tgccgagaac cgcgcccaaa gataatattc cgccctttcc atgccaatta cctttctgaa
     1981 cataggtctg accgacattt gacgaactat tattgagaat aacatgaccg tttttctcca
     2041 gagtcatgtt acctgtgaca atgacggggt caggcaaaaa aggggcatta atattattga
     2101 ttagcacagt cccactattt aataaagtgt taacagtttg gttcatgcct gaaaaattca
     2161 aggtagcacc tttattaaca ataacaccag cggtacgtgt catagcttca actgtcccca
     2221 tttttagaat gcctgcattg atgttggtga aaccagagta ggtatttttt tccgccaaaa
     2281 tgagagttcc ctcccctgat tttgttaggg attttccgtc ccagctattt atattatgat
     2341 ggctgagatt atctgccaga atagtactaa cagtaagttc agcgttttct ttcagattga
     2401 actctccatg actgtcgcca tctgtatcat tccagctcaa accgtagatc aatttattct
     2461 ggtcactgat atagcttttt gtatagtcaa tagcaccagt agtgagtaac ggattgatga
     2521 tagatatatt agaaacactg aaacctgtta tccctttatc tgcacttagc agtttatagt
     2581 ccttctgcgc ccagttattt ataatagcct ccacttttac attgttgcca aatgacatat
     2641 ttccagaagc ttccagacta ctgattccag ctaaatttaa agaactgttc tcaccaaaag
     2701 aaacgtcacc tgaaacagtg aatttgttac ctgatagtga aacagttgta caatcaccag
     2761 cacataaatc accttcaata gttggtgtgg tcccagtgta atcattgttg ataattttca
     2821 gaatggatga attgttaacc tgtacatcac cagtaatgac agaaccttca tggagtatta
     2881 atgaattgtt tccaccagtg atgtggatag cattacctgc tacattttgt gttccatcat
     2941 cttctttacc tgaaattata tatccgctat tagcaatggt catattgcta ccatttatag
     3001 catcccccca atgtccacca ttaccgccta aaatagctcc attgttgatt acagacaggt
     3061 tatctcctgt gatagcatta ccaccgtagc catcataacc ataaccgtaa gcagcacctc
     3121 cgttgcctcc agcgaacgtg cccttattga ttacagagag gctgcttcct gtgatggcat
     3181 cacctccatt accgccatca ccctcaccat aattgttacc tccattacca ccggaaatag
     3241 tccctttgtt gattatgggt aaattgactc ctgtgacggc atcacctcca ttccccccat
     3301 cactatcact ataactatcg ccaccatgtc cacctgaaat aatttctcca tttattataa
     3361 acagattgtc tcctgtaaca gcctcaccac catcaccgtt atggtcagca ccgctaccgc
     3421 cgctaccacc aagaatcatg ccttgattga ttatagacaa gtcagatccg gtaatagagt
     3481 caccaccatt accgccacaa ctattaccat cattattatc accagcacca ccatgacctg
     3541 cgctaatcat aatatcagag ttaatccgta gcttagttct agatgcatga gaggggttgg
     3601 aagaagttat ataccacgca tccattccat cttcaccagc tcccaaggga gaaagtccat
     3661 caacaggtgt taataatttt tcataattgt cctctgaaaa atgaagttct tgagtacccg
     3721 aaagaggagt agcaaaagct attggccccc cgagcaacag ggatgcacca acaacaaaag
     3781 gagaaaaaga caatttgcat gttttttcct ttaccgtagg taattctccg gcccccccct
     3841 gtgaacattg ggtcatatta caaaaaaatt tgtgaatttg attcatgcac tatattatca
     3901 gtaagtggtt gataaacccc tgaaaagatt ccgttttatc cggaataaag ggacgatata
     3961 tgcaaaaaca tattaaacaa agcctcaacc aaaacatgag agctgtgtta tatatcaatg
     4021 caacgggaga aatttacctg tgtcttgctt tgaaaaaaag agggggaaag ctatatgttc
     4081 gataataaat taataatttt actatgatag tctgcttcag accgcaactt ttcagttctt
     4141 tctgtacgct tgcccaaagt ttaaccactt tgtttttcta tacacccaat ggttctatct
     4201 gccccttgat gttaatcccc aaagccaata acatgaatgt attcatatca caactttcct
     4261 accagactct ccaatgcaat aactgcataa ttgtttttag tggaaatgta tcgttttctt
     4321 aaagagaaga ataacattcc attttattat gttcccaatt taattatatt aatatatatt
     4381 ggcattaata ggaaaataca tcaggagaaa tcaaatgcag acatcaaaca taactaacca
     4441 tgaaagaaat gactcatcat ggatgtcaac tgtcaaatca actacagaag taagttggaa
     4501 taaattgagt ttttgtgaca ttttactgaa aataataact ttcggcatat attctccaca
     4561 cgaaactcta gccgaaaaac atagcgaaaa aaaactaatg gatagtttct caccatcact
     4621 atcacaagat aaaatggacg gagagtttgc ccacgcaaac atagatggca tttcaatacg
     4681 gctatgcctt aacaaaggca tatgttcggt tttttatctt gatggagata aaatacaatc
     4741 cactcaactt tcatcaaaag aatataataa cttgttaagt tcactccccc ccaaacaatt
     4801 caacttagga aaagtacata ctattacagc tccggtatca gggaatttta aaactcacaa
     4861 gcctgcacca gaagttattg aaacagccat taattgctgt acatcgataa taccaaacga
     4921 tgattacttc cacgttaaag acactgattt taatagtgtt tggcatgata tttacagaga
     4981 tattcgagca tctgacagca atagtacaaa aatatatttc aacaatattg aaataccact
     5041 aaaattaatt gcagatctaa ttaatgagtt aggaattaat gaattcattg atagcaaaaa
     5101 agagcttcaa atgctaagtt acaaccaagt aaataaaata atcaattcaa acttccccca
     5161 acaagaccta tgttttcaaa cagaaaaact tttgtttact tcactctttc aagaccctgc
     5221 atttatatct gcactaacat ctgcattctg gcaatctctt cacatcacgt cttcctctgt
     5281 ggaacacata tatgcccaaa ttatgtccga aaacatagaa aacaggctta atttcatgcc
     5341 tgaacaacga gttattaaca attgtggaca tattataaaa atcaacgcag ttgtccctaa
     5401 aaacgacact gcaatctctg cctctggggg gagggcttac gaagttagct catcaatatt
     5461 accatcgcac ataacatgta atggtgttgg tattaataaa atagaaacaa gctatctcgt
     5521 tcatgctgga acacttccaa gcagtgaagg gcttcgcaat gctatccctc cagaaagtcg
     5581 tcaagtatca tttgccatca tatctcctga tgtttaagcc aatattatat tggctgccag
     5641 actgtaaaaa aacgttcttc agaacagctc tataaactat taaaccaaag ctaaaaggat
     5701 ggggaccaat agaatctcag cccatccttt ttatatcatt atgaaccgcc ccggaaatcc
     5761 tggagactaa actccctgag aaagaggtaa acaggatgac taaaaataca cgtttttccc
     5821 ctgaagtccg tcaacgggca gttcgtatgg ttctggaaag tcagggcgaa tatgactcac
     5881 aatgggcagc gatttgttcc attgccccaa agattggctg tatactggaa actctgcgtg
     5941 tctgggtacg ccagcatgag cgggataccg ggggcggtga gg
//
LOCUS       DAJTSF010000170         5889 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000170 DAJTSF010000000
VERSION     DAJTSF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.173"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            50..244
                     /locus_tag="OO335_003852"
     CDS             50..244
                     /locus_tag="OO335_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR6004046.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
     gene            511..1437
                     /gene="tauA"
                     /locus_tag="OO335_003853"
     CDS             511..1437
                     /gene="tauA"
                     /locus_tag="OO335_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6004047.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="OO335_003854"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="OO335_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6004048.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="OO335_003855"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="OO335_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6004049.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="OO335_003856"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="OO335_003856"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR6004050.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="OO335_003857"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="OO335_003857"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6004051.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5493..5861
                     /locus_tag="OO335_003858"
                     /pseudo
     CDS             5493..5861
                     /locus_tag="OO335_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1399 a         1454 c         1598 g         1438 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat
     5101 agcaataatt tcatattcac tgaaaatatt tttaatcttt atttatagct cttggttatt
     5161 attttttggc gtttggttgt gctgctataa attgaataat tcaaactttg ttgtatattg
     5221 ataagatatt tatctggttt gtatttttga tatttataac gcgtatgcat caaaaataac
     5281 attatctgca acatactgaa atatcgtcct tttatcaatt tgatgcgtgt ttttctccat
     5341 aaattatata tgtccacatt cggacttagg ggaaagaata attgaaccat tcgtctgtaa
     5401 cgcagcataa tcgttagcgc gaaacataat atgttttcta tgcattgata attgatggat
     5461 caacttatta cgtccctgag gagggatgac aaatgcactc ctggaaaaag aaacttgtag
     5521 tatcacaatt agcattggct tgcactctgg ctatcacctc tcaggctaat gcagcgacca
     5581 acgatatttc tggtcaaact tacaatactt tccatcacta caacgacgcc acctatgctg
     5641 atgacgttta ctatgatggt tatgtaggct ggaacaacta tgccgctgat agctattaca
     5701 acggcgatat ctacccggtc attaataacg ctaccgttaa cggcgtgatt tctacctact
     5761 atctggacga cggtatttct accaatacca acgccaatag tctgacaatc aaaaacagca
     5821 ctattcacgg tatgatttat tccgagtgca tgacaactga ttagactggc cccctgaatc
     5881 tccagacaa
//
LOCUS       DAJTSF010000171         5805 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000171 DAJTSF010000000
VERSION     DAJTSF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.174"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="OO335_003859"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="OO335_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR6004052.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="OO335_003860"
     CDS             578..1027
                     /locus_tag="OO335_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6004053.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1261)
                     /locus_tag="OO335_003861"
     CDS             complement(1028..1261)
                     /locus_tag="OO335_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR6004054.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="OO335_003862"
     CDS             complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="OO335_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR6004055.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1715..1810
                     /gene="yncL"
                     /locus_tag="OO335_003863"
     CDS             1715..1810
                     /gene="yncL"
                     /locus_tag="OO335_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR6004056.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2212..3021)
                     /locus_tag="OO335_003864"
     CDS             complement(2212..3021)
                     /locus_tag="OO335_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR6004057.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3231..4655)
                     /gene="patD"
                     /locus_tag="OO335_003865"
     CDS             complement(3231..4655)
                     /gene="patD"
                     /locus_tag="OO335_003865"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR6004058.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4677..5471)
                     /locus_tag="OO335_003866"
     CDS             complement(4677..5471)
                     /locus_tag="OO335_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6004059.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5461..>5805)
                     /locus_tag="OO335_003867"
     CDS             complement(5461..>5805)
                     /locus_tag="OO335_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6004060.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1522 a         1474 c         1287 g         1522 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt gtatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagtcg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcattggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg cgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcgac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt
     1081 ctttatgctt gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc
     1141 gatcttcccg acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg
     1201 caatgacgct ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca
     1261 tactttctcc ttagccactt gtgaaattta tcgattaatc caaatgatat aagtctgcgt
     1321 ggtgccgtca agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa
     1381 ctcatcggcc atgtggcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg
     1441 tgagaaagaa accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc
     1501 atatgttgat agagaaacat gtacgggcgt ccagccatat gaataaaacg cgcgcaaata
     1561 tgcgccattt tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca
     1621 gcaacttgcc cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact
     1681 atacacatag gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt
     1741 actgataaat gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg
     1801 gtttatttga tgtagtgatt ttgcacgcag cacgtactgt cagagaaatc aactctctga
     1861 cagtacgcaa gatcttctca tactctccaa gtttgcaaca ttatccttaa tatttacttc
     1921 tttttgctgg catctacatt gaaatgagta gaaaacatcc ccctactgtc gtctatacaa
     1981 acagcttact gattcaaacg gaatcttgtt ttcacatttt tctatcacga atactttata
     2041 cgcgcgggag tctataaatg ctttatactt tcaaggaata acgtaaatat aaaaagatgg
     2101 cgtaaagtat atgcatggca ggatgtttgc aacctccttg atgacgctgc cccattgaaa
     2161 ttgcaaaccc cgccctggtt atcttgccca tttatcatgc aaaacagata attataggcc
     2221 tctgaatacc tcagaaagat tgaatttttt atagtccctc tcttgaatat ttccatttga
     2281 tatcaatcga ttcatctctt caacaaaata atttatatca tcacacttat tattatccgg
     2341 aagatctgag tagttataat tccaccattt tattctcaac agttcatcaa taacatttga
     2401 atcaaaccta aatctgatta tttttgcagg aactcctgca actatggcat atgggggcac
     2461 atctttggta acaacggaat tggcagcaat aacagcacca tcacctattg cgatgccacc
     2521 tttcaataca acatcatttc ctatccagac atcatttcct ataatcggat tttcaggttt
     2581 ggtaatgaat ggtttcaatg tccagctacg ttcaaattca ctcatagcaa acttatcaaa
     2641 ttcaccgtta taagtcatca tgtgagtagt aaaacgattc aaaggatgat ttccacccat
     2701 tatttttaca tttgatgcaa tactacaaaa tcgacctata cgtatattcc ctgaaaaatg
     2761 gcaggtcgga aatgaaaaag cgccgacact ggagaaattt ttcacaggca ttgttgaata
     2821 ttcttcagca ataacattat cttcaattgt tatcttgtca ccttttgaga aaagaaataa
     2881 attcctttct ttatacgact tcttcccctt atgatttaaa aagattttat tatccataca
     2941 aaactcaaag tgttttttcg tccatctgaa ggtaagggtt ttatttttat cttccgtgaa
     3001 tttgtacaat tgaactttca ttgttaatac ttctacttat aaagtattag tttaataacg
     3061 atactctaaa gccttatgct aatcaactgt gacaggatga aaaatcgcca tcaaacattt
     3121 agcctaattt ttacgccagt tattaatgta atcattctaa tacttaaccg ccattaaact
     3181 cagaacctaa aacgatagtg gccgtcctat aacagccact aacatctaac ttaatgttta
     3241 accatgacgt ggcggacgac ggtgtaatcc tccagcccat aaagtgacat atccttgccg
     3301 taaccagaaa gtttctgccc accgtgcggc atttcactta ccagcatgaa atgggtattg
     3361 acccaggtac aaccatattg cagccgtgcg ctgacgcgat gcgccctgcc cacatctttc
     3421 gtccataccg aagatgcaag tccgtactgg ctgtcattcg cccagttcac cacctgttct
     3481 tcgttgtcga agggcgtaac actcactact ggaccaaata cctctttttg cacgatggca
     3541 tcgtcctgta atgcgccagc cagcagcgtc ggcgcatagt aatagccatt acccttgcgc
     3601 ttttcaccgc cagtgatcac tttgatgtgc cctgtcgctt tcgcctcttc tactgccttg
     3661 ctgacgcgtt cgagatgcgc cagcgagctt aaaggtccaa gctccgtaga ctcgtcatcc
     3721 ggcgcaccag attttaacgt tgccaccgca gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaagata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatac ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 acccgttctc cgttaatcag taacttatgt tgcatagctt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg
     5281 gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc
     5341 ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg
     5401 ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt
     5461 tcagagtgca tcgaacgctc ccagacgttt cacgaacgcc aggtacagtg cgatgagaat
     5521 aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga
     5581 ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc
     5641 gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg
     5701 cagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa
     5761 caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggat
//
LOCUS       DAJTSF010000172         5738 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000172 DAJTSF010000000
VERSION     DAJTSF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.175"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..241
                     /locus_tag="OO335_003868"
     CDS             <1..241
                     /locus_tag="OO335_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004061.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="OO335_003869"
     CDS             complement(238..1179)
                     /locus_tag="OO335_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR6004062.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="OO335_003870"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="OO335_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="OO335_003871"
     CDS             complement(1684..2904)
                     /locus_tag="OO335_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR6004063.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="OO335_003872"
     CDS             complement(2908..3651)
                     /locus_tag="OO335_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR6004064.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="OO335_003873"
     CDS             complement(3542..5008)
                     /locus_tag="OO335_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR6004065.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5241)
                     /locus_tag="OO335_003874"
     CDS             complement(5008..5241)
                     /locus_tag="OO335_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR6004066.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5229..5708)
                     /locus_tag="OO335_003875"
     CDS             complement(5229..5708)
                     /locus_tag="OO335_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004067.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1226 a         1618 c         1435 g         1459 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DAJTSF010000173         5734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000173 DAJTSF010000000
VERSION     DAJTSF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.176"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..163
                     /locus_tag="OO335_003876"
                     /pseudo
     CDS             1..163
                     /locus_tag="OO335_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            688..1866
                     /gene="ltrA"
                     /locus_tag="OO335_003877"
     CDS             688..1866
                     /gene="ltrA"
                     /locus_tag="OO335_003877"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6004068.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1949..2128
                     /locus_tag="OO335_003878"
                     /pseudo
     CDS             1949..2128
                     /locus_tag="OO335_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2158..2640)
                     /locus_tag="OO335_003879"
                     /pseudo
     CDS             complement(2158..2640)
                     /locus_tag="OO335_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2896..3876)
                     /locus_tag="OO335_003880"
     CDS             complement(2896..3876)
                     /locus_tag="OO335_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6004069.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3876..5075)
                     /locus_tag="OO335_003881"
     CDS             complement(3876..5075)
                     /locus_tag="OO335_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6004070.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5397..>5734)
                     /locus_tag="OO335_003882"
     CDS             complement(5397..>5734)
                     /locus_tag="OO335_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6004071.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1572 a         1315 c         1366 g         1481 t
ORIGIN      
        1 gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta aagtccatgc
       61 cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga ctgcccggct
      121 gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat aagttatcca
      181 tgctctgagg tgggtagagg tgggccctcc ggtcagggtt tacaggagca cagaccaaac
      241 gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa aaggcagaga
      301 tctactgtca cgtgggttcg ggaagctgaa cgaaagtaaa ctgtccgatg aagcatcgaa
      361 atcttagatg atgccaaaat ggggccgtac agccgtcccc tgataagcgc agaagacacc
      421 tgcttactga ctgcgcggca tccggtgtga aggcagcagg atcccgggcc agaccatctc
      481 agggaacgtg agaacctgtt tccggcacgt atcgggaaac aggggcggac ccattcgtag
      541 tagtgatgaa agatctgtaa tgggtctgga gcgaagggat ggggatcaca ttcattgcag
      601 aagacacaac ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt gaaataccaa
      661 aagcactggt ctgggcatca tatctggatg tgcgtcgcaa caagggagcc ccccggatgc
      721 gacgggcaga ctttgaaaat gttcgaccag caacgtgatg gcaatctata caagatctgg
      781 aaccggctat gttcgggaac atggtttccg ccaccagtgc tggaaaaacg gatcccgaag
      841 tcgaatggca aggaacgcat tctggggatc ccaacagtat ccgatcgaat tgctcaggga
      901 gcgataaaac ttttcatgga agaaaagctt gacccgattt tccatgctga ttcatatggt
      961 taccgaccag gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg gtgctggcgt
     1021 tatagctgga tactggaagt ggacatcagt gcgtttttcg accatgtgag acatgatctg
     1081 gtgctcaaag ctctggaaca ccacgggatg cctaaatggg tcatcctgta ctgtcgacgc
     1141 tggatggaag cgccaatgca gagttgtgaa aatggagaat taataacccg aacacgaggc
     1201 actccgcagg gcggggtcat tagtccgcta cttgctaact tgttccttca ctatgcattt
     1261 gatttgtgga tggaaagaga atatcggggg gtaccgtttg agaggtacgc tgacgatatt
     1321 gtagtacatt gttcacgaat gagtgatgca acaaggctaa agaacaggtt atcggagcgc
     1381 ttctcggaag tcgggctggt cctgaatgca gggaaaacga atatcgccta cattgacacg
     1441 tttaaaagga gaaacgtcgc aacgagtttc acctttctcg gatatgactt caaagtgcgt
     1501 acgctgaaga atttcaacgg cgaacggtac cgaaaatgca tgccgggtgc gtcaaatgca
     1561 gcaatgcgca aaataacaga aacaatcaag aagtggcgta tacatcgctc aacagctgag
     1621 agtttgctgg attttgcgag gcgctacaat gcgatagtga gaggctggat cgagtactac
     1681 ggaaagttct ggtccagaaa tttcaactat cgactgtgga gtgcaatgca gtcacgtctg
     1741 ctcaagtgga tgcagtctaa atacagactt tcgaaccgga aggctcagcg aaagctgacg
     1801 ctggtaagga aggagtatcc gaagctattt gttcactggt atttactacg tgcatcaaat
     1861 gagtgatcaa gagccgtatg acgcgagagt gtcacgtacg gttccgtgag cagccgggga
     1921 gtgaaattct ctccggctga ctcgactcgc gggttccgac agcgatgacg aacatgcggc
     1981 ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggaaccag aaaaatggct
     2041 gcgttacgtc attgagcata tccaggactg gccggcaaac cgggtacgtg atctgttgcc
     2101 ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag ccgcttactt
     2161 ttggcgtaat tctgctgcgc ctttactgaa cacaatgccg tattcagcaa gaagtccacg
     2221 aatgtgattt atcagttgtg tcctttccat tgaaaccaac cgacgcatat tcaaaaaagt
     2281 ctggacgtcc tgttgctcca gagtttttat tggtacgggt cgtatagttc catgttggca
     2341 ggcctcagct attgcctgtg cgtcgttata atcatttttc tggcaccgct gataagcttt
     2401 aacatgttgt gccggaagca atattacatc atggtcgagt ttcctgattt cccgggccca
     2461 gtaatgagat gccccgccag cctccatcgc aacagtcatt ttaggctgtt gcaccagcca
     2521 gttaagcagt tgattgcgag aaagtttggt ctttcctgcg ggtttgccat gagcattgat
     2581 actgaaaaga taaaagttcg ttttagccag atcaatgccg gtgattttct gttcagacat
     2641 ggtatcccgc ttacttttgt tgttaaagtt tatccatcat ggcgcaattg aaggctgccg
     2701 tgaaagaggg ggaagactat ctcatcggtt actgttaaac ggctaagaga aactatcttt
     2761 ttcaagcaaa gaagatacgg tgaaacaggc gatttcaccg tgaaatactg ttcaaaaaaa
     2821 acgctaataa aagcaaaatt tcttttaaaa cagccaatta aaaactggcg agttgctggc
     2881 gaaaaacttg aattttcaca gcgaattcag attatctttt agtacaagag caatcatacg
     2941 atctaactta tcctgaactt ccaacggtaa tctgccgaat tcatatgaga acgtacgtcc
     3001 ctttactcgt tttcgagcaa atttatcttt actatcaaat ttccataaca aagttacaac
     3061 cgctttatcc tttactcctg aatcaagcaa tgaagacatt tccttggtta taagccgcat
     3121 aatactgttt ttaacctcgt cttcagaaag attgtcatca gcagtaataa gctcaatact
     3181 cggcgagata ttcttcacaa cttcatcaat agatatatca cccagcctca gtcgttcact
     3241 taattcagcc agttgcctgt aatcggcgta tgtcagctca gactgaacag gaaaaagaga
     3301 cacaaaatct ttaggtacgg acgctgcttg gagggcacga gtcaccttag ctgccgacaa
     3361 tccctctctt tccgcaattt gcttctggtt cataccggcc tctttgagac gtactaaacg
     3421 caacccaatt tcacgaatat tatgttcaag tgacgtctgc aaatctttcg ccagatgctg
     3481 agcttcagaa acagtaagct catcatccgt tacaagtaca cgtaatccaa ccttgcatag
     3541 caaagctgct gcccgacgac gggaaccatc caaaatctca ataagatcgc cagtccgaat
     3601 cccaatacat ggatagaatt gttgtaaacg tatagttctg gtgatgtctt ttagtgagtc
     3661 cggcgtcagg gctgtctgtt cgcggccatt cacctcttca accacaaaag tttgagtatc
     3721 cactttgtca tgatcaacct tgatctcagt aaattttgcc tttcggccag tattgagggt
     3781 aaatacatga actggagccg atatttcttc agtattattc aggatatttg tattcagcgt
     3841 acggccaata gtcggtcggt gctttctgtt ttccatcagt agttggccct tataaattca
     3901 atacgatcaa aaactgcttt agcgaaatct tctgctgcca tacgtgcatt tttcaaagct
     3961 tcaccactgc caacataagt aacaggattc gcagatatca cggtatcaaa cgactcgccg
     4021 cttctctcaa aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc accgaaaatc
     4081 tcttttgtca ggctatggca gtatttatga tctgacttat tcgccagttt cgacatgaag
     4141 ccaatatttg cctgcaaacg gcatgaacat ccatcctgtt caataatctg tacaagttca
     4201 ggtaaacgag ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg cggcaccggt
     4261 gtaaacatga tatctgcggc agcaatggca tttttcaaaa atgcgtccag atgcgggcct
     4321 gtatcaataa ggatgaagtc gaaatcatgc ttcagcttat caataatatt ctcacgcaat
     4381 atcgcgtgtt tgttttgtcc taatagatgc tcctcgcaca gagtatccca gtttgatgca
     4441 ataaaggcgt cgtcaataga agccggcata acataaacgc cagggataac tgacggaacg
     4501 ataaaatctt ccagtaattc ctcacgactc acgttctgca acatagcctg cggtgcggtt
     4561 gtatcaacca gccccaccgc atgcaagtag ttcagaaaca tagtggctga tgactgggga
     4621 tctaaatcaa ggagcagaat acgtaaatct tctgacagca ggtgaggatg agcacgcagg
     4681 gcgtgagcta cggaaacact actcacggtt ttagacacac cgccttttag actaccgatg
     4741 aatatggaat atgcctcact ataacgatcg cgatatttag gaatgccacg atgtgcatag
     4801 atatcgataa tgttctggat ggttaatgca tattttgtcg cggttcctgc tgggcgtttt
     4861 tcaaactgat agccctgagc ctccatctca ccgactgcac catccacgct tgcgcgcgtt
     4921 aattttggaa gcttagctac agcagacttt gaatagactt gataaaaaac atcagcctga
     4981 aactcctgct tttgctcctg aatctgtttt gttagggcga gcaacatttt atctgcacgc
     5041 tgcgcaacct tacttaactg ctcaaaacta gtcattcccc ctccgtgcta tgtaggattt
     5101 taacttatta caaaaaaaac tgcattagca gggtaatgca tgcaagtaca tattgtacat
     5161 gcttatatta aaaatgcgcg cattgctgat ttattaatca tcggaatgaa tattactgta
     5221 agatagtatg cctttccggg agaaagacgc tgatttcccc cgaataatcc agttcaccac
     5281 cacactccgg acaacagctt tcttcaggct ccagatggtg tatttcgcgg gaaacatgtt
     5341 ccggtaacgg acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc ttccaatcag
     5401 tagaacatgt gcttttcaat gaaggagccg atgacttttt cgtggatttc cacggaacgc
     5461 gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt ccggttgtta
     5521 cgtgcaatac gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg cacttctctg
     5581 gcgtagctgc cccagtcatc gctggtcacc atgccgatac agaacggagt caacagggct
     5641 aacagttccc gacaggtctc atcattccgt ggcccgaaag tgtaagccaa cacaccgcca
     5701 gttttagtgt tgtacgcata ccagagccag tgtt
//
LOCUS       DAJTSF010000174         5731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000174 DAJTSF010000000
VERSION     DAJTSF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.177"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(160..900)
                     /gene="pflA"
                     /locus_tag="OO335_003883"
     CDS             complement(160..900)
                     /gene="pflA"
                     /locus_tag="OO335_003883"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR6004072.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="OO335_003884"
     CDS             complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="OO335_003884"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6004073.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3429..4286)
                     /gene="focA"
                     /locus_tag="OO335_003885"
     CDS             complement(3429..4286)
                     /gene="focA"
                     /locus_tag="OO335_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6004074.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4692..>5731)
                     /locus_tag="OO335_003886"
     CDS             complement(4692..>5731)
                     /locus_tag="OO335_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6004075.1"
                     /translation="SPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGAHPDFGVALE
                     RTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDLFKQDADYPF
                     VDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSDIYPAEDLWL
                     ANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLGLATGSDNGW
                     YTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQTLLLLAQEE
                     DRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAHQSLLKAYEK
                     LQRAKAAFWAK"
BASE COUNT         1496 a         1406 c         1381 g         1448 t
ORIGIN      
        1 ttcgatgggg aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat
       61 atggtagatg cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa
      121 aaagcggcaa ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc
      181 gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc
      241 atcgagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg
      301 gagaagttcg attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc
      361 cgaatcgtca tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt
      421 tttgttcgcc agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt
      481 ttggtggatc tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag
      541 cagttcatca atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg
      601 aatgccttct tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc
      661 ttcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac
      721 cacttccttc atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc
      781 gcggttatga caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat
      841 acctgggccg tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat
      901 tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta
      961 cgtcgagtct gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt
     1021 gctaaaaaag gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca
     1081 gtcaaatcta attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct
     1141 ttagtcagcg agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat
     1201 ttttccgggt tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg
     1261 tgctgaccac cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg
     1321 ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag
     1381 gagataccat ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca
     1441 gcacctttct ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca
     1501 cgacgaccgt ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc
     1561 agaacagact gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg
     1621 aaacgttcta ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc
     1681 gggtattcgc cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta
     1741 actttcgcat atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca
     1801 cacgccatgg tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg
     1861 tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag
     1921 tccatgaagt gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc
     1981 ggttcagact tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg
     2041 tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg
     2101 atcatcgggc ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc
     2161 aggtcatcgt tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc
     2221 ttgaagttca gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc
     2281 cccatagtgt acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca
     2341 tcgaggccca taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca
     2401 tcgtattccg gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc
     2461 atttcctgcg cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca
     2521 tccaggaagg tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca
     2581 gccaggtagc cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag
     2641 atgtcgtagc cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca
     2701 gcgatttctt cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc
     2761 tgtagagagg tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca
     2821 acgcgacggt agtcaccgat gatacggcca cggccataag catctggcag accggtcaga
     2881 acaccggatt tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg
     2941 tgagttttac ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac
     3001 gctttgcagg aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc
     3061 ggagcttcag tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg
     3121 tgagaggtga tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt
     3181 tccagtttaa caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca
     3241 gccaggaagg actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg
     3301 tttacttcat tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca
     3361 ttaagctcgg acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc
     3421 gacaaccatt aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc
     3481 aacaaaccac cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag
     3541 ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc
     3601 ggagatgcga agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg
     3661 tgctcaaaac cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc
     3721 atcaggctgc ggccagaata actcatccat actgccagac ataccatcag gtttgccagg
     3781 ataccaagac agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg
     3841 acgtttagtc cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt
     3901 acaaacagca gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac
     3961 tgacaccagg tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag
     4021 agatcggctc cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc
     4081 agttttgcca tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg
     4141 aatgcgattg agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt
     4201 ttcgttgctt tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa
     4261 agatcaaaag ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat
     4321 actaacaaag cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc
     4381 ccgtaactcg catggttttt atgcaaatac ggagtaacta tttgattatc caaataaaaa
     4441 taaattttaa aaattaacaa atgagttgaa ttttttccgc atcctccgct aaaacagtta
     4501 attaaaaggg agcatcaggc gaataaagta acaatatcga tcgtatttat taaatacaaa
     4561 ttaccgatat ttaaccttat aattacaatt attttattaa tgcaaatata tgtaaagcgg
     4621 ggcattaaaa aaacgccccg taatataact cagactaatc attaagccta cattgcgtag
     4681 gctatttgat tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc
     4741 ttcaacaacg actgatgtgc agcaaacgcg tgcagatcgc tatctaccgg ttgcaggccg
     4801 taaaacgccg cttcgccgct cattgccgca ctggcggctt ctacggcatc tgcgccgtac
     4861 atgcgaacaa acgcattcag atattgcagc ggctggcgat cttcttcctg tgccagtaat
     4921 aacaacgttt gcaggcagcg ataatagttg gcgcgttccg gactaaatac tgatgagtta
     4981 aactccatcg tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc
     5041 agcatggctt ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc
     5101 agacccaaca gctcacgcac gcgggtaaag tcatcaaaac cttcttcatc cagttgctcg
     5161 atgaggttca ggtaatcttc tttttcccac tcgctgcctg gtagcgaaag aatcgtttca
     5221 cgtaaatggc tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg
     5281 gacatgccag gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata
     5341 taaacttctt tatcttcttt gttgaagata gccatcagcg tagcaaactc ttcttccgtg
     5401 gtgccggaga aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag
     5461 gagattaaac cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct
     5521 tcatcatcga aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc
     5581 agctcggtca cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca
     5641 aagcaggtgc cgtttgcagg attaaacagt accacgcaaa tcaccggata ctggccgcca
     5701 agggaaccat catatgcgaa gatcggggaa c
//
LOCUS       DAJTSF010000175         5702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000175 DAJTSF010000000
VERSION     DAJTSF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.178"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..170)
                     /locus_tag="OO335_003887"
     CDS             complement(<1..170)
                     /locus_tag="OO335_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004076.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="OO335_003888"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="OO335_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6004077.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="OO335_003889"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="OO335_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /locus_tag="OO335_003890"
     CDS             complement(3616..4383)
                     /locus_tag="OO335_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6004078.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /locus_tag="OO335_003891"
                     /pseudo
     CDS             4579..5487
                     /locus_tag="OO335_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            5484..>5702
                     /locus_tag="OO335_003892"
     CDS             5484..>5702
                     /locus_tag="OO335_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR6004079.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAJTSF010000176         5600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000176 DAJTSF010000000
VERSION     DAJTSF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.179"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..411
                     /locus_tag="OO335_003893"
     CDS             <1..411
                     /locus_tag="OO335_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6004080.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="OO335_003894"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="OO335_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO335_003895"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO335_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR6004081.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1332 g         1488 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc
//
LOCUS       DAJTSF010000177         5557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000177 DAJTSF010000000
VERSION     DAJTSF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.180"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..641
                     /locus_tag="OO335_003896"
     CDS             <1..641
                     /locus_tag="OO335_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR6004082.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="OO335_003897"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="OO335_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6004083.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="OO335_003898"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="OO335_003898"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6004084.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1359 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatcg
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagatatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaa
//
LOCUS       DAJTSF010000178         5520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000178 DAJTSF010000000
VERSION     DAJTSF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.181"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(139..3654)
                     /locus_tag="OO335_003899"
     CDS             complement(139..3654)
                     /locus_tag="OO335_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6004085.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSHKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="OO335_003900"
     CDS             complement(4107..5369)
                     /locus_tag="OO335_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6004086.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
BASE COUNT         1412 a         1370 c         1203 g         1535 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttatgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
//
LOCUS       DAJTSF010000179         5485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000179 DAJTSF010000000
VERSION     DAJTSF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.182"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1630)
                     /locus_tag="OO335_003901"
     CDS             complement(<1..1630)
                     /locus_tag="OO335_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR6004087.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2530)
                     /locus_tag="OO335_003902"
     CDS             complement(1646..2530)
                     /locus_tag="OO335_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6004088.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2541..3146)
                     /locus_tag="OO335_003903"
     CDS             complement(2541..3146)
                     /locus_tag="OO335_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6004089.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5485)
                     /locus_tag="OO335_003904"
     CDS             complement(3317..>5485)
                     /locus_tag="OO335_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCR6004090.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1581 a         1364 c         1141 g         1399 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccca gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgttt
//
LOCUS       DAJTSF010000180         5398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000180 DAJTSF010000000
VERSION     DAJTSF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.183"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..497
                     /locus_tag="OO335_003905"
     CDS             <1..497
                     /locus_tag="OO335_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR6004091.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="OO335_003906"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="OO335_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="OO335_003907"
     CDS             2500..3114
                     /locus_tag="OO335_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR6004092.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="OO335_003908"
     CDS             3114..3443
                     /locus_tag="OO335_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR6004093.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="OO335_003909"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="OO335_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR6004094.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="OO335_003910"
     CDS             4529..4840
                     /locus_tag="OO335_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6004095.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4975..5373
                     /locus_tag="OO335_003911"
     CDS             4975..5373
                     /locus_tag="OO335_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6004096.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1177 a         1344 c         1425 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggca
//
LOCUS       DAJTSF010000181         5337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000181 DAJTSF010000000
VERSION     DAJTSF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.185"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO335_003912"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO335_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6004097.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OO335_003913"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OO335_003913"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6004098.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OO335_003914"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OO335_003914"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6004099.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OO335_003915"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OO335_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR6004100.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1289 c         1457 g         1281 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctatg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc acccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcaacag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggg
//
LOCUS       DAJTSF010000182         5181 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000182 DAJTSF010000000
VERSION     DAJTSF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.186"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..746
                     /locus_tag="OO335_003916"
     CDS             <1..746
                     /locus_tag="OO335_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6004101.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="OO335_003917"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="OO335_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6004102.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3810
                     /gene="safA"
                     /locus_tag="OO335_003918"
     CDS             3607..3810
                     /gene="safA"
                     /locus_tag="OO335_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6004103.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="OO335_003919"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="OO335_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6004104.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5041..>5181
                     /locus_tag="OO335_003920"
     CDS             5041..>5181
                     /locus_tag="OO335_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6004105.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDF"
BASE COUNT         1480 a         1102 c         1156 g         1443 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg taattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctggaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctccgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     4741 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat gattatttat
     4801 gatctcaatt cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag
     4861 ttgtttatcg gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact
     4921 gttatttccc tttttttagc taaatctgct atctctttat gtgactaact tcacttacat
     4981 ccacttattt ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt
     5041 atgaagtctg cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct
     5101 ggtatggctg catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca
     5161 aacgttgctt tctcagactt t
//
LOCUS       DAJTSF010000183         5162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000183 DAJTSF010000000
VERSION     DAJTSF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.187"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..220
                     /locus_tag="OO335_003921"
     CDS             <1..220
                     /locus_tag="OO335_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6004106.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(392..721)
                     /locus_tag="OO335_003922"
     CDS             complement(392..721)
                     /locus_tag="OO335_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR6004107.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(726..911)
                     /locus_tag="OO335_003923"
     CDS             complement(726..911)
                     /locus_tag="OO335_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6004108.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(908..3547)
                     /locus_tag="OO335_003924"
     CDS             complement(908..3547)
                     /locus_tag="OO335_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6004109.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3755..4744
                     /gene="ldhA"
                     /locus_tag="OO335_003925"
     CDS             3755..4744
                     /gene="ldhA"
                     /locus_tag="OO335_003925"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6004110.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4855..>5162
                     /gene="hslJ"
                     /locus_tag="OO335_003926"
     CDS             4855..>5162
                     /gene="hslJ"
                     /locus_tag="OO335_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR6004111.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     "
BASE COUNT         1307 a         1322 c         1203 g         1330 t
ORIGIN      
        1 tttgtaccga atgtttgccg ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg
       61 tctcgatttt tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat
      121 cggctttcat tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt
      181 tgggatgacg ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgcatgtac
      241 tgcaacgcca attagttaaa acagatattt gactatgcat ttttcagggc gaaaggcaat
      301 ttgacaggag tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct
      361 gtagcgttat cccgatattg cggttttgct attaaaactt tcgcacccat ttaccgttta
      421 tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg gcgagtttcg
      481 cgatatcatc tacagacaca ttattttgct ttgcccgttg ttggtaactc gctttacgtt
      541 cggcattaat ttcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat
      601 aaccataaaa tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca
      661 ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca
      721 tcgcctcaga aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg
      781 atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc cttaggtgca
      841 gcgacttcaa tgcgaggcgt acagccaacc agcataaaag atgacgtcaa cgcagccagt
      901 aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac
      961 gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg
     1021 taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc
     1081 gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct aagttaattt
     1141 ttgtcgatga ccggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca
     1201 tattgtcttt caccaccgca tctgcggtat ctttatccag ccgcaaggtc attagcccgc
     1261 tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg
     1321 caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc
     1381 tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt
     1441 tcatcagcac gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt
     1501 cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat
     1561 taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat
     1621 cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg
     1681 cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag
     1741 aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca
     1801 ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa
     1861 taccgtccca gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt
     1921 taaagaggaa ataggtcgga ttgcgcccat cgacgctaaa ttttaaggtt gatggcggta
     1981 acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg
     2041 agggatgctg tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt
     2101 ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa
     2161 tcgtgctgtc atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa
     2221 aactcccttt tccccagtag cgccactgcc agcggccagc gtcagggaga aaatcattcg
     2281 ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg
     2341 cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga
     2401 tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct
     2461 caaaggtcag cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata
     2521 aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagttga ctgttatcca
     2581 tgcttaattt tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg
     2641 tcagcacatt cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga
     2701 ctttgacacc cagtcggcca ctcaaaggaa aaccagcata cagccagctc cagcgaccat
     2761 cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt
     2821 tatcccgtgc cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca
     2881 ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc
     2941 catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc tgattttcaa
     3001 acgcgacgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt
     3061 taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt
     3121 ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag
     3181 gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg
     3241 gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc
     3301 tgggatgtga aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac
     3361 ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac
     3421 gtgtcccgag cggtagccag atgtccgcca gcgtgggaac ccacagcccg agcgtcatca
     3481 gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac
     3541 ccagcatggg tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg
     3601 tggcatgttt aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat
     3661 ttttcaatat cgccatggct ttcaattata tttgaaattt tgtaaaatat ttttagtagc
     3721 ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa
     3781 acagtacgac aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt
     3841 ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg
     3901 tattttcgta aacgatgacg gcagccaccc ggtgctggaa gagctgaaaa agcacggcgt
     3961 taaatatatc gccctgcgct gtgccggttt caataacgtc gaccttgatg cggcaaaaga
     4021 actggggctg aaagtagtcc gtgttccagc ctatgatcca gaggccgttg ctgaacacgc
     4081 catcggtatg atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga
     4141 cgctaacttc tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt
     4201 tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg
     4261 tctgctggcg ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt
     4321 cgatctgcca accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc
     4381 ggaaaactac catctgttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat
     4441 cgtcaatacc agtcgcggtg cattgattga ttctcaggcg gcaattgaag cgctgaaaaa
     4501 tcagaaaatt ggttcgttgg gtatggacgt ctatgagaac gaacgcgatc tgttctttga
     4561 agataaatcc aacgacgtga tccaggatga cgtattccgt cgcctgtctg cctgccacaa
     4621 cgtgctgttt accgggcacc aggcattcct gacagcagaa gctctgacca gtatttctca
     4681 gactacgctg caaaacttaa gcaatctgga aaaaggtgaa acctgcccga acgaactggt
     4741 ttaatcttgc agctcccctg caatccaggg gagctgattc agataatccc caatgacctt
     4801 tcattctcta ttcttaaaat agtcctgagt cagaaactgt aattgagaac cacaatgaag
     4861 aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa
     4921 attgctgtta cgccagaaca gctacagcat catcgctttg tgctggaaag cgtaaacggt
     4981 aagcccgtga ccagcgataa aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt
     5041 tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca
     5101 gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat
     5161 aa
//
LOCUS       DAJTSF010000184         5151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000184 DAJTSF010000000
VERSION     DAJTSF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.188"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            190..447
                     /locus_tag="OO335_003927"
                     /pseudo
     CDS             190..447
                     /locus_tag="OO335_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO335_003928"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO335_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6004112.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO335_003929"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO335_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6004113.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO335_003930"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO335_003930"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6004114.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="OO335_003931"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="OO335_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3605..4420)
                     /locus_tag="OO335_003932"
     CDS             complement(3605..4420)
                     /locus_tag="OO335_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6004115.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4517..>5151
                     /locus_tag="OO335_003933"
     CDS             4517..>5151
                     /locus_tag="OO335_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6004116.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1374 a         1175 c         1226 g         1376 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctggcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaatataccc t
//
LOCUS       DAJTSF010000185         5114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000185 DAJTSF010000000
VERSION     DAJTSF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.189"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(337..582)
                     /locus_tag="OO335_003934"
     CDS             complement(337..582)
                     /locus_tag="OO335_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004117.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1359..2201)
                     /locus_tag="OO335_003935"
     CDS             complement(1359..2201)
                     /locus_tag="OO335_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR6004118.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2286..2483)
                     /locus_tag="OO335_003936"
     CDS             complement(2286..2483)
                     /locus_tag="OO335_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004119.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2503..2991)
                     /locus_tag="OO335_003937"
     CDS             complement(2503..2991)
                     /locus_tag="OO335_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004120.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(2988..3365)
                     /locus_tag="OO335_003938"
     CDS             complement(2988..3365)
                     /locus_tag="OO335_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR6004121.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3412..3789)
                     /gene="cbtA"
                     /locus_tag="OO335_003939"
     CDS             complement(3412..3789)
                     /gene="cbtA"
                     /locus_tag="OO335_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCR6004122.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3869..4090)
                     /locus_tag="OO335_003940"
     CDS             complement(3869..4090)
                     /locus_tag="OO335_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR6004123.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4177..4653)
                     /locus_tag="OO335_003941"
     CDS             complement(4177..4653)
                     /locus_tag="OO335_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR6004124.1"
                     /translation="MQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(4669..>5114)
                     /locus_tag="OO335_003942"
     CDS             complement(4669..>5114)
                     /locus_tag="OO335_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6004125.1"
                     /translation="SACAPETDLQQLVATLVPDEQRISFWPQHFGLIPQWVTLEPRVF
                     GWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAG
                     IAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT         1192 a         1243 c         1356 g         1323 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgttgg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctgggtggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gccggactcg gaatcgaaaa tttcttttat
     1261 catattgata ttaatgcaat ttgtttggtt tgattaaaaa tgtaccctca attatacccg
     1321 cagtcccttt tgatatcgaa agggaatcac tctcgggatc aggctgagag cacagaagga
     1381 aagtacctgg ctattatatc gttaattctg tcaatcagtt ccagcctttt gaaagtgatg
     1441 tgtgcagatc ccttcttaaa gtatttaatg atgaacatct cgtcttcata gcgctcttta
     1501 ccctgaacgg actggatgtg accatccaga cgaatagtga tgttttcccg gttgtcgggg
     1561 atcggtttgc cgctgaacag atgtagcatt ctttccaggt cgacgagtcg gtcagtctgt
     1621 tgcccggtga taagatgaaa cccccaccgg tcccacctca ccaggttgtt gacgataatt
     1681 ttactgccaa atttacaggg tgagttggtt ttgtaatccc agctcagccc tttgaagacg
     1741 ttgatcactc cccgttcaaa cacttcatct ttgttctggt gcagttgttc aaatgtgctc
     1801 agaatgttcg cttcgctgat ttccggaaaa ttatcatatt ccagtgacct gtaccatgtg
     1861 tcccgggtct gcgcatccat cagactcagc atcccggaac gttgcatcag atcacgccag
     1921 agttcgcgat caagattgcg tgtgatggct ttcattgcgg tttccggttt ctccatcagc
     1981 cagcaaccgc agcgaaaatc ctgtttcatg gcccagtcca gagccgtttt acctctaata
     2041 ctttctgtca ttgctgatac ttcagccagt tgtcggataa gtgtgtcgat ttgcagcaat
     2101 gtactctttc gtacagccag aatgtggcgg atactggttg agcagataac gtccgtatgc
     2161 ccgttgacca cttccggacg agaggctgtg atttcagaca taatcgatat tcctttttgc
     2221 agataaacga aaacgccagc cgtattcagg ctggcgtcag tggaatgaag tctggtgagt
     2281 aagactcacc ggttctgttg cagaaactgc agctgacgca gggtacggat atcttccctg
     2341 gcctgttcaa tacagggcag cagtgctgcc gaatccgttt tatcctcatc gttgtcgaag
     2401 ataatccctg attcgcagtc aatattgtcc tgcagccacg caatcagaat atccagcgcc
     2461 gtttctgtgg ttaataattt catccagtgt cttttgtcct gattaccagt cgaaagtggg
     2521 gaaccccggc agacattctg cgccggcgtc cagatgaatg agactgacgc cataacgccg
     2581 ggtgagagtg acgaccagac gacggaacgt tctggacagg cccaggcgtt taagccgcag
     2641 aaccgggtac gaccatgcat cggtacgtaa cagatagccg ctgcctgtaa aatgaatcca
     2701 ttctgtttcg ccgtcgtcat tagtctggtg tgacagcgag tacagccagg cgttgtccgt
     2761 ttccgtgata tgtgcggtgc tgcaacgtat gcaggtaagg gttgttaacg gtggtggtgt
     2821 actggttgcg tgttggtcgg atttatcgac cacgcggagt gaataaccgt tttcagagac
     2881 cttgttaatc agttcactga gattaacacc atcgacgtca acgacaatgc gccccatgtt
     2941 cagtgcctgc acgttaacgc tgtcggcttc cagcgtcagg gccagtttca ttgtttcgct
     3001 tccggatgct tcccccgggt aatgttattt accgttctgt agttatcgcg ggtcatcagg
     3061 ccggttgccc ggcgtgcccg gagaatatca atgctgttga ttaactgcga cggggctcct
     3121 gcgctgaatc ccggctggtc ggtacgtacc agcgcgtatt tttcaacgag aaagttcacc
     3181 gcgtcacaca gtgaaattcc tgcctcaata tgctgctcaa tcacacgttc atcagcgaac
     3241 ggtgtgtcat tcagcgtgag gccatagtgc tggtccagca gtcgggtgag cagtgtctgc
     3301 cagatggtga tgggagacgg gcagcccgat gcctcccgta cgtgagtgtc gggtaatgta
     3361 ttcatcttaa gttctctgta acaggtggtt ataaagggga gatatccggt gttatacggt
     3421 ggttgcgggc gctgccggtg tcggataaac agccagataa acgtaaccac aggagccgag
     3481 ggtgtcggct tcgcaggtca gccctttcgc atacagtgtg acggtatgct gatggcgggg
     3541 attcagttca ccgccagtga gcatgagttc cagttgtttc atcagcagcg gaaaggcctg
     3601 gtccaggtgg tacgcatcta cgtcgctgaa cctgcctctg ataccggcgc ggtctgcaag
     3661 gtaatgcagg cggttaccct cctgtaccag tcgggccccg aaacagggcg tcacggtgca
     3721 gggcagcccc caccaggggc ggtcgtgatt gtcgtcggga ggcgttgtcc cggggagtgt
     3781 gtctgacacg ataaagtcct cacagaaaat cggttaagaa tggtccggta gtggcgataa
     3841 ttctgctcat cagaattccc ttccagactc agttcagggt gacgctcatc agccggacat
     3901 acggaccaaa actgtcctta cggcgttctg caaacacagc cagtacaccg ggaatatcct
     3961 gcacttcacg gccggtatat gtatccgtgc taccgtgcca gcggtattta ccggtacaga
     4021 acggaaaaag acgggatgca ggatgctgac ggtgaatacg catggcttca ccacgggtga
     4081 tgattttcat gagaatagtc ctcctgaatg tgaaagtaag aaacagctga gaaagagaaa
     4141 ctgaagtgat atgaaggtga ttgtgacggt gacgggttaa agcagaccgt gttccgcaaa
     4201 ggagaaaacc tggctgccac cgactatcag atggtccggt acccggatat caaccagggc
     4261 cagtgcctgt accagacgct cagtgataag gcggtctgcc ttgctggggg ggacttcacc
     4321 ggacgggtga ttgtgcgcca gcaccacggc agcggcattg tggtacaggg cgcgtttaat
     4381 cacttcccgg ggatggactt ccgtacggtt gatggtgccg gtgaagaggg tttcaccggc
     4441 aatcagctga ttctggttgt tcagatacag cacccggaac tcttcacgct ccagtcccgc
     4501 catgttcaga atcagccatt cccgtgccgc atgcgtggag gtgaaggcca cgccgggttc
     4561 atgaagatgg cggtccaggg ttttcagggc ccgcagaatg aggctgcgct ctctggttgt
     4621 catttctccg ggcagaaagg aaagctgctg cattgtccct gccaccattc agtcgatgat
     4681 gcgcataatg gcgctgcatt ccggatgctg cagggcgtaa tcccgcagcc ggtaataatg
     4741 gaccgtcatg gcataacact ccgtacgaca ggcatgatga ctgtacgtca tcagacaggc
     4801 agcaatgccg gcagcttccg gactcatttc agcgcggtta ccgttcatgg cattgaacag
     4861 tacccatgtt tcgtcatcat cgtcatccgg ttcgggtgcc ataaatgccc cgccgttgtt
     4921 cagggtgtac agattccaga taccaccgca gtagtcttcg cacagacggt ccatccagcc
     4981 gaagacacgg ggctccaggg tcacccactg tggaatgagg ccaaagtgct gcggccagaa
     5041 gctgatgcgc tgttcatcag ggacgagggt ggcaaccagt tgttgcaggt cagtctccgg
     5101 tgcacaggcc gaag
//
LOCUS       DAJTSF010000186         5024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000186 DAJTSF010000000
VERSION     DAJTSF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.190"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="OO335_003943"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="OO335_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR6004126.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="OO335_003944"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="OO335_003944"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR6004127.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="OO335_003945"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="OO335_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR6004128.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="OO335_003946"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="OO335_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6004129.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>5024
                     /gene="yidZ"
                     /locus_tag="OO335_003947"
     CDS             4378..>5024
                     /gene="yidZ"
                     /locus_tag="OO335_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6004130.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1144 a         1208 c         1256 g         1416 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     4981 cgttatccgc atatcagcat ttgctgggaa cagagcgaca cctg
//
LOCUS       DAJTSF010000187         5014 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000187 DAJTSF010000000
VERSION     DAJTSF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.191"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..297
                     /locus_tag="OO335_003948"
     CDS             <1..297
                     /locus_tag="OO335_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6004131.1"
                     /translation="LFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADN
                     QQVKLSMPELEELAAAMVQAQVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(498..1355)
                     /gene="sitD"
                     /locus_tag="OO335_003949"
     CDS             complement(498..1355)
                     /gene="sitD"
                     /locus_tag="OO335_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6004132.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1352..2209)
                     /gene="sitC"
                     /locus_tag="OO335_003950"
     CDS             complement(1352..2209)
                     /gene="sitC"
                     /locus_tag="OO335_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6004133.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2206..3033)
                     /gene="sitB"
                     /locus_tag="OO335_003951"
     CDS             complement(2206..3033)
                     /gene="sitB"
                     /locus_tag="OO335_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6004134.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3033..3947)
                     /gene="sitA"
                     /locus_tag="OO335_003952"
     CDS             complement(3033..3947)
                     /gene="sitA"
                     /locus_tag="OO335_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6004135.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4379..>5014)
                     /locus_tag="OO335_003953"
     CDS             complement(4379..>5014)
                     /locus_tag="OO335_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6004136.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1323 a         1227 c         1200 g         1264 t
ORIGIN      
        1 ctgttcacgt ttgaatggaa cggtcgtatc tggaatgctg gtcccgactc actgggccgc
       61 ctgtccccgg tagtcatgct ggcaaaatct gtcacagcac aaacacatat ggcgtggagc
      121 gatgccgata atcagcaggt gaaactgtcg atgccggaac tggaagaact ggcggcagca
      181 atggtgcagg cgcaggtcga tcgcaacgat gagatttatc gccgtcagcg ggagctgaag
      241 gaagagctga atagtctgaa ggatttgaat tcggttagga attttatcgt ggaataacag
      301 aagctgcggc acgtcgtatg caggaacgtg ccgcggttgg ctggtaaact ttcgatagtg
      361 cgagtattga atgatttcta ggcgttatcg attttacgta ttttttgcat gatagaattc
      421 gtacctcctc ccaacgacct tccatggctt tccggacttc tgtaggctgg cggacgatgc
      481 tgtcctcgga tgttgtgcta attgggtgat aacagatcct gtgtatgggc gtttcccttt
      541 gtgcgtgcgt tgatgctggt aacggtaaac gtcatgatga acacgaccgc gaataagacg
      601 acaatcgtcg gagctggtgc gctgtcgata aaaaaggaca gccagacacc agtcattgac
      661 actattaccg atatgccagt cgccagtagg agcgcaatat gaaagcgttg tgtcaggagt
      721 actgcaatgg cgccaggcgc aatgagcaga gaaatagaga gaatgatacc tactgctttc
      781 agcgtcgcca caatggtcag ggaaaccatg cacagcagcc cataatgcag ccatctcgta
      841 tgcaaaccgc ttacctgcgc ctgttgataa tcaaagctga acagcaaaaa atcccgccat
      901 tttacgctaa taacaagtgt aaccagcccg gcaataatca ccgtctggat tatgtcgccg
      961 atggttatcc cgagcatgtc gccgaaaaga atatggtcaa gatgtacgtc tggtttaatt
     1021 gcaatataca agatcagacc tgcggcaaac atgccagaaa aaacaatccc cataactgta
     1081 tcttgcttga ttcggctgtt gtctttcagg tatcccgtcg ccaccgcgca gaatactccg
     1141 gcgacgaatg cccctgtagc cagaggtaac cccagaatcc aggtaagcac gatgccagga
     1201 aaaaccgcat gactcatcgc gtcacccatc agtgcccaac ctttcaggac cagaaagacc
     1261 gacagcagcg cacagggtat ggaaactacc agtgaaatca gcagtgcgtg actcataaag
     1321 gtgaactgca gtggctccag taataatgcc atcatgagcg atcctccagc gctttatgcg
     1381 cacgccgacg atttgccaaa agtccatggg tgggcgcaaa tacaaatgct aaaagaaaca
     1441 gcagtgtctg cgccaccacg atgattccac ctgtcgcgcc gtccagaaag taactgaccc
     1501 acgcgccgag gaaactggtt acactgccaa tagttacagc aattatgagc aggcggggaa
     1561 aacgatcggt taacaaccat gctgttgcac cgggagtcac caccagacag atcaccagaa
     1621 aggcaccaac cgtttgcagc gccgcaacgg tcgaaaccga cagcagggtg aagaagagga
     1681 tttttaatct gcccggatgt aatccaatag cacgggcgtg attctcgtcg aaaaaggtca
     1741 ccataagatc tttccatttg aaaaaaagca cgatgatcga gagaatgcca atgatagtga
     1801 gttgcaggat atccgccgga tcgattgcca gaatattgcc caggacgatg gtctgaatgt
     1861 tcacggatgt cgggttcaat gacaccataa acagccccag gccaaaaaag gacgagaaga
     1921 tcaggccgat gatggcatct tcttttaagc gggtgcgttg gttaagaaac agcatgctgc
     1981 ccgctgccag tccgccggaa aagaaggctc ccagagaaaa cggaagcccc agcatatatg
     2041 cccccgcaac acccgggaca atagagtgcg acagcgcatc gccaatcagc gaccagcctt
     2101 ttagcatcag atagcatgac aggaaagcgc agagaccgcc gaccattgcc gagacccaca
     2161 tcgcattgag catatattca tagctgaagg gttccagtag cacgttcatc ttgcttccct
     2221 ctgcaccgct gacggtcgat gtgccacaaa agggcgttca tcgtcagtaa taatgctttc
     2281 ttccgagccg ttaagtgtga catggcgcag tacgccgcta aaagccagct ccaggttctc
     2341 ggctgtaaaa gtggtgtctg tcggcccgct tgccaggacg gtgcctttga ccatgaccgt
     2401 ataatcgcaa aatgtcgtga ctgagccgag attgtgcgtt gagacaagca tcgttttgcc
     2461 ttccgcgcgt aactccctca acaggctgat gattttcgct tcggttttca catcaacgcc
     2521 ggtaaaaggt tcatccagca ggatcacatc accctgctgt gcaattgctc tcgccagaaa
     2581 gacgcgtttc ttttgtccac cggaaagctc gccgatttga cgatggcgaa aatccaccat
     2641 atcgacacgt tccagcgcat cggtgacaat ctgacgatcc ctctttttgg ctatacgcaa
     2701 catacccata tgcccatagc ggcccatcat caccacatct tccaccagta caggaaatga
     2761 ccagtcaacc tcttctgact gagggacgta ggcaaccagg tttttctgta gcgcctgtcg
     2821 cgtgggaata cccagaacag atatctttcc gctggtcaga cgcacaaatc ccataatcgc
     2881 tttaaacaac gtcgattttc cggaaccgtt taccccaacc agagcggcaa tagatccact
     2941 tggtactgtg aaggatgcat cgcgcagtgc tgtgtgcccg ttacgccagg tgacagtaac
     3001 atcattaacg acaatgcctg cagattgcat cattatttct cccttttccc ggctttaatt
     3061 ccctgtacca gcgtactggt agtaacctta agaaggtcga tatacgtagg taccgggccg
     3121 ttttctgtgc tcagggaatc gacatagagc acaccgccgt agtgtgcgcc ggtttcacgc
     3181 gcaacctgac gcgctggttt atcggaaatc gtactctcgc taaagactgc cgggatatga
     3241 tttttcttaa ctatatcaac aaccttacgt acctgctgcg gtgttccttg ttgatcggca
     3301 ttaatcggcc acagataaag ctcttttagc cccaaatcgc gtgcgagata agaaaaagcc
     3361 ccttcactgg tgaccatcca tcgctgattc tcagggagtt ccgtaatctg cttacgcagg
     3421 ggggcaaggg tttgggtaat cttggcttta taagtatcgg cattacgttg gtaggtttgt
     3481 gcatttgccg gatcgtattt tatcaacgca tcacgaatat tatcgacgta aatcagagca
     3541 ttatctggcg acatccatgc atggggatta ggtttgccct catagggtcc ttcggtgatc
     3601 cctactggcg tcacacccga agagacaatt acttctggaa ccccattgag atgctggtaa
     3661 aagcgttgga accacaattc cagattcata ccattggcga gaatcagttg tgccccctgc
     3721 gcacgtttaa tatcgccagg ggtaggctga tactcatgaa tttctgcacc aggcttggtt
     3781 atggatgaga cttctgcagc atctccagcc acgtttttgg ccatatccgc gatgatggta
     3841 aatgttgtaa tgaccttgaa tttttcagtt gcacttgcct gaaatgcgat cgagcaggtg
     3901 agggcgaggc accccaagag catggttact ttttttattg agtgcataat ttagtaccta
     3961 tagtgagcaa aattagaaca aacatagcaa cggctatgtt ttatagcata acctatacaa
     4021 aatgaaaata attatcatta acagctttga ggcctaaaac agggattagg ttgtttcctg
     4081 ctggcgacat gatattgagg aaaaaagtag agcagctatt aggctttgaa atgactttat
     4141 gaggaaaatt gttgatggca aaaatggggc aaaaaagagt gggttatcgt agcttattgt
     4201 tgtcgctgat aatgtttaac atattgaaaa ataaataaaa tacttatgag tcagatagtt
     4261 gtgatttttg cccttacttg ttcaggttgt attgttcttt ctttgtaatt tgttgatttt
     4321 cttgcattat ttcagttctc tggtactaaa tggggcaaat tgggggcaaa ctttgcaact
     4381 acgataaccg cgcattcaac atggctatct gttcgtcgtt catgtcatca atccacatac
     4441 cgtaaatttc atacaccatt tgcgcagttt catgccccat ctggctggct ataaatgccg
     4501 ggttcgctcc tgccgtcaac agccagcagg caaaagtatg tcgcgtatgg tacggattac
     4561 ggcggcgaat accagcacgt tttactgctg cattccatct tgtacccaaa ctgcttaccg
     4621 agtaataagg tttctgtttt tcgttacaca ccctgggcat gaaaacaaaa tgcagttttt
     4681 gtttttcagt tctgccgtac tcccgatgat aaaaagtgat ttcgcttttg cgatgatgcc
     4741 cggtcagttt gtattgctcc ttcagtgctt caagagccgg ctgcagtaat gctaccgtcc
     4801 ggatcccggc atttgttttt gggggaccga acatatcaag tatcgtcagg tttcttctga
     4861 cattcacaat tcccttctcg aaatccacat cctcccacgc cagagctgcc agttccccgt
     4921 gacgaagccc ggagtaaacg gcaaatttcc acaagttttg gctctgtcct ttttcacttt
     4981 ccattaatgc attgaattct gttttagata acgg
//
LOCUS       DAJTSF010000188         4941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000188 DAJTSF010000000
VERSION     DAJTSF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.192"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(605..1021)
                     /locus_tag="OO335_003954"
     CDS             complement(605..1021)
                     /locus_tag="OO335_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004137.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1048..1188
                     /locus_tag="OO335_003955"
                     /pseudo
     CDS             1048..1188
                     /locus_tag="OO335_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1188..2213
                     /locus_tag="OO335_003956"
     CDS             1188..2213
                     /locus_tag="OO335_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6004138.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2266..2355)
                     /locus_tag="OO335_003957"
     tRNA            complement(2266..2355)
                     /locus_tag="OO335_003957"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2319..2321),aa:Ser,seq:cga)
     gene            2449..3246
                     /gene="mtfA"
                     /locus_tag="OO335_003958"
     CDS             2449..3246
                     /gene="mtfA"
                     /locus_tag="OO335_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6004139.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3347..3422
                     /locus_tag="OO335_003959"
     tRNA            3347..3422
                     /locus_tag="OO335_003959"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3380..3382,aa:Asn,seq:gtt)
     gene            3584..4846
                     /locus_tag="OO335_003960"
                     /pseudo
     CDS             3584..4846
                     /locus_tag="OO335_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1372 a         1160 c         1107 g         1302 t
ORIGIN      
        1 tgttcagcat ggattgagcc tcagattaat gttaagcgtc ttgcaggacg cgtaatgtta
       61 actggggctt ttctctatct gccttttggt gttcatgcct gagacagata gcctcaagca
      121 cccgcagtca ttctacttaa ctaagatttc cctgcaaacc gtttttgtcc ggcacagtaa
      181 atatccaact aaaccaatag cgttcgctgt atttaccgcc agtattcaat gcacatgacc
      241 gccatgaaca cccctaaaaa aagggcattt atatgtccaa acattaatat caaaacatca
      301 attttttcca tataccttgc tgtgaagatg atgggcatac atgatgcgaa caaccagaac
      361 gcaacaaaca aaaactgcaa tgcgtttttc attattcccc ctacaatcaa tgtgcaataa
      421 catttaaaca cacctcaatt tggccggaca tataaatatc taaaccagaa aaaatcactt
      481 acatagcgtt acaaactctt tagtctaaat actcatcgta aaacattccc catacttatc
      541 agtccgttcc gcgccaggta gcttattgcc ttatctggca acctataatc aggtttccgc
      601 tttttcagtt ggctggtcgt ttaaccgaca tagttaaccc attaatctgg ttgccggatg
      661 ctggtggatt ttcgcgtttt aattgttcat aaaagtgcac agctttaacc agttcttctg
      721 atgtaaccgg gactggtggg gcagtgaata aggcctgaat ttcatagttc ggcctgtcgt
      781 tacaatcctc ttttttcggt acatatttcc agtcaccaac ccacaactcc ccctgagagt
      841 ccataacacc ttttttcacg tagcgatatc gccacgctat cggctctgct tccagcgatg
      901 cccgtgcaat tttgaataac tcgccctcta ctcgcgccat ccctgaattg gggtggcatt
      961 tcgtaatcgc tatttttaat ttggcttctt cgattaattg ttcttttgtt aattcagtca
     1021 ttttcattac cgccctggta atgctgccac cagcgaaaat ggttaatgga aaacgggctt
     1081 ccgttctata ccaatcgtag tggcaaaccg attgtcagcc gggatctatt tacctgcaat
     1141 aaaactttac caccacgcga ggtagagccg aattttggtg cgatctgatg ggaagacgaa
     1201 ggaaaaatcc tgaacacgaa aaattacctc caaatgtata cccaaataaa tatagttatg
     1261 tatggaaacc aacatccaga gaatctgtaa cactaaccgc catcaaggat ggtttagctg
     1321 ctttatggaa aaagtatgag gaaactgtaa ataatcgcga tcgtgcaatg acattcggtc
     1381 gcttgtggga aaaattcctc gccagcgcct attacagtga cctcagtcca agaacacaaa
     1441 aagattatct gcaacatcaa aaaaagttgc ttgccgtatt cggtaaggtg ccggcagatt
     1501 ccataaaacc agaacacatc cgtcgataca tggacaagag aggggaacag agtaaaacgc
     1561 aagccaacca tgaaaaaagc agtatgtccc gcgtttacag ttgggggtat gagcgagggt
     1621 acgtgaaggc taacccatgt gcaggtgtaa gtaaattcaa ggccaaaaac cgcgaacgat
     1681 atgtaaccga caaagaatac caggcagtat taagcgttgc acctcttcct gtttttatcg
     1741 caatggaaat tgcctatctg tgtgcagcga gggtttccga tgtgttatcg ctgaaatggg
     1801 agcagattgg aaacgacggg atctttatcc agcaagggaa aacaggaaaa aaacagataa
     1861 aagcatggag tccacgatta caggcggcga tcgaaaaagc aaaacagtta ccaacatccg
     1921 cctatgtaat cagcaatcaa tacggcaacc gatatatgta caaaggcttt aacgaaatgt
     1981 gggtagaagc aagaaatcgc gcaggcaaaa tttcaggtat tttaaccgac ttcacctttc
     2041 atgatctgaa ggcgaaagga atttcagact atgaaggaag cagtcgggat aagcaacttt
     2101 tctctggtca caaaaccgag gggcaagtgc taatctatga caggaaggtt aaagtttcac
     2161 cgacacttga tgtcccgtta cctgaaaata ttccaagaaa atattccaag taattccaag
     2221 tgtgattttt gtcactgact taatgatgta taagtgattg aattttggcg gagagagggg
     2281 gatttgaacc cccggtggag ttgcccccac tccggttttc gagaccggtc cgttcagccg
     2341 ctccggcatc tctccgttca gatggttgcc atgatgccag gaaatttggc attttaacag
     2401 tccctgtccg tgcaattttg ttcaagtgac gagtttgcga gcaaaacgat gattaagtgg
     2461 ccctggaaag tacaagaatc agcacatcaa actgcccttc cctggcagga agcactatcg
     2521 atcccccttt taacgtgtct gacagaacag gaacaaagca aattggtcgc tcttgccgaa
     2581 cgttttttac agcaaaaacg gcttgttcct ttacagggct ttgagctgaa ttcattaaga
     2641 agctgccgga tagcacttct attttgcctg cccgttctgg agttaggact ggaatggctg
     2701 gatggttttc atgaagtctt aatttatcct gcgccatttg tggtcgatga tgaatgggaa
     2761 gacgatatcg gtctggtgca taaccaacgt attgttcagt caggtcagag ctggcagcaa
     2821 gggcctatcg ttttgaactg gttggatata caagattctt ttgatgcttc tggttttaac
     2881 ctgattattc atgaagtcgc tcataagctg gacacccgta acggcgatcg cgccagcgga
     2941 gttcccttta tttcgttgcg tgaggttgct ggctgggaac acgatcttca tgctgcaatg
     3001 aacaacattc aggaagaaat cgaattagtt ggtgagaatg cggcgagcat tgatgcttat
     3061 gctgccagtg atcctgctga atgttttgcc gtactttctg aatatttctt tagcgcccca
     3121 gaactttttg ctcctcgttt cccttcattg tggcaacgtt tctgtcaatt ttatcaacaa
     3181 gatcctttgc agagactgca tcacgctaat gatacagact cgttttcagc gacgaatgtt
     3241 cattaattaa caactttgaa gattaattaa ccaattgaaa tggcttatga aatttagtgt
     3301 tgacagacaa ggtaccgcta agtaatatgc gccccgttct cacgattcct ctgtagttca
     3361 gtcggtagaa cggcggactg ttaatccgta tgtcactggt tcgagtccag tcagaggagc
     3421 caattttctg ttttcatgca tccttgcgaa tctttatctg atgttgattc aacaggttag
     3481 tgtgaaaact cttctcggtg cattttgatt ttaccctacg catcgggaaa aattggtggt
     3541 caaatctggg atcaggttag ttcgataatg gagtgacccc catatatccc ttaccgacgc
     3601 aaaaatccgc accctcaagc cttctgataa accctttaaa gtctccgatt ctcacggtct
     3661 gtatctgctg gtcaagccgg gtggctcccg ccactggtat ctcaaatacc gtattagcgg
     3721 taaagaatcc cgcattgcgc tgggtgccta tccagccatc tccctgtctg atgcgcgaca
     3781 gcaacgtgaa ggtatccgta aaatgctggc gctgaatatc aacccggtac agcagcgggc
     3841 tgctgaacgt ggctcacgaa caccggagaa agtttttaaa aacatggcgc tggcgtgaca
     3901 taaaagtaac aggaaatggt cgcagaacac cgccgaccgt ctgcttgcca gcctgaacaa
     3961 tcacatcttt ccggtcatcg ggaacctacc tgtatcagaa cttaaacccc gtcatttcat
     4021 tgacctgctg aaagggatcg aggaaaaagg tctgctggag gttgcgtccc gcacacggca
     4081 gcacctgagt aacataatgc gccatgcggt ccatcaggag ttaatcgata cgaaccctgc
     4141 agcaaacctt ggcggcgtga ccacacctcc tgtcagacgg cactatcctg ccctgccgct
     4201 ggagcggctg cctgaactgc ttgaacgtat tggggcatat catcagggcc gtgaactgac
     4261 ccggcatgcc gttctgctga tgctgcatgt gttcattcgc tccagtgaac tgcgtttcgc
     4321 ccgctggtca gagattgatt tcacaaaccg agtctggacg atacccgcga cgcgagaacc
     4381 cattattggc gtgcgttatt ccggccgcgg ggcaaaaatg cgaatgccgc atatcgtccc
     4441 cctctcagaa cagtccatcg ccattctgaa acagattaag gatatcaccg gtaataatga
     4501 actgatcttc cccggcgacc ataacccgta taagccaatg tgtgaaaaca cggtcaataa
     4561 ggcactgcgg gtgatgggtt acgacacgaa aaaggatatc tgcggtcacg gcttccgggc
     4621 aatggcatgc agtgcgctga tggaatcggg tttatgggca aaggacgcag tagaacgcca
     4681 gatgagtcat caggagcgca ataccgtgcg catggcttat attcataagg cagagcacct
     4741 ggaagcccgc aaagcgatga tgcagtggtg gtcggattat ctggaagcat gccgagaatc
     4801 ttatgcaccg ccttatacaa ttggtaaaaa taagtttatc ccatagtata ctaacgaatc
     4861 ccacactctg gcaaattacc accacctcta caggaggtgg ttctatatag acttttatca
     4921 acttcatgac gaaaaacacc t
//
LOCUS       DAJTSF010000189         4920 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000189 DAJTSF010000000
VERSION     DAJTSF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.193"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="OO335_003961"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="OO335_003961"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR6004140.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="OO335_003962"
     CDS             1020..1703
                     /locus_tag="OO335_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR6004141.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="OO335_003963"
     CDS             1700..2161
                     /locus_tag="OO335_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR6004142.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="OO335_003964"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="OO335_003964"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6004143.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="OO335_003965"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="OO335_003965"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR6004144.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO335_003966"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO335_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR6004145.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO335_003967"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO335_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR6004146.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1152 a         1295 c         1234 g         1239 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
//
LOCUS       DAJTSF010000190         4870 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000190 DAJTSF010000000
VERSION     DAJTSF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.194"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..527
                     /locus_tag="OO335_003968"
     CDS             <1..527
                     /locus_tag="OO335_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR6004147.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="OO335_003969"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="OO335_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR6004148.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3205)
                     /gene="gap"
                     /locus_tag="OO335_003970"
                     /pseudo
     CDS             complement(2205..3205)
                     /gene="gap"
                     /locus_tag="OO335_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3394..3924
                     /gene="cybB"
                     /locus_tag="OO335_003971"
     CDS             3394..3924
                     /gene="cybB"
                     /locus_tag="OO335_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR6004149.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4169..4342
                     /locus_tag="OO335_003972"
     CDS             4169..4342
                     /locus_tag="OO335_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004150.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="OO335_003973"
     CDS             complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="OO335_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR6004151.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4664..4804)
                     /locus_tag="OO335_003974"
                     /pseudo
     CDS             complement(4664..4804)
                     /locus_tag="OO335_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1120 c         1208 g         1295 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 ttccgagaat ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     2521 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
     2581 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg
     2641 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     2701 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg
     2761 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga
     2821 tggttttcat ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat
     2881 gcgcctgcga tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
     2941 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3001 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     3061 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     3121 cgtctatgtt gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa
     3181 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     3241 gatcctttcc aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa
     3301 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     3361 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     3421 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     3481 cgtggtttct tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc
     3541 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
     3601 ccgattatac ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg
     3661 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
     3721 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     3781 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
     3841 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     3901 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
     3961 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
     4021 gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac
     4081 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac
     4141 ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
     4201 aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
     4261 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc
     4321 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
     4381 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
     4441 agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
     4501 tgtgaatgcc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt
     4561 catagccttt ctccttgccg gatggcgggt aagaggctaa caatctgaat tgcttggttc
     4621 attcgttggc ctcggtttgt actgacccca aaaagttgga cagttaaaca cgaggcatat
     4681 aggtctgatt ccgatattca tttggagtca gaccttttaa tttcaggcta attcttctgc
     4741 tgttgtagta ttcaatatat tccgtaacag catccttcag ttcgcttata ttactgaact
     4801 cattagactg gccccctgaa tctccagaca accagtatca cttatttaag tgatagtctt
     4861 aatactagtt
//
LOCUS       DAJTSF010000191         4866 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000191 DAJTSF010000000
VERSION     DAJTSF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.195"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1100)
                     /locus_tag="OO335_003975"
     CDS             complement(<1..1100)
                     /locus_tag="OO335_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR6004152.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OO335_003976"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OO335_003976"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6004153.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OO335_003977"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OO335_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6004154.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OO335_003978"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OO335_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6004155.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OO335_003979"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OO335_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6004156.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OO335_003980"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OO335_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6004157.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="OO335_003981"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="OO335_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6004158.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..>4866)
                     /locus_tag="OO335_003982"
     CDS             complement(4501..>4866)
                     /locus_tag="OO335_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6004159.1"
                     /translation="PLAVAAAHAVLNIIDKESLCERANQLGQRLKNTLIDAKESVPAI
                     AAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLT
                     IPDAQFDAAMKILQDALSD"
BASE COUNT         1205 a         1230 c         1159 g         1272 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaataatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggaagggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccatgtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg agctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaaaaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataacttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgg
//
LOCUS       DAJTSF010000192         4769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000192 DAJTSF010000000
VERSION     DAJTSF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.196"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            109..293
                     /locus_tag="OO335_003983"
                     /pseudo
     CDS             109..293
                     /locus_tag="OO335_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            436..657
                     /locus_tag="OO335_003984"
                     /pseudo
     CDS             436..657
                     /locus_tag="OO335_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(678..740)
                     /locus_tag="OO335_003985"
                     /pseudo
     CDS             complement(678..740)
                     /locus_tag="OO335_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1190..2602
                     /gene="ospC1"
                     /locus_tag="OO335_003986"
     CDS             1190..2602
                     /gene="ospC1"
                     /locus_tag="OO335_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR6004160.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2706..2855)
                     /locus_tag="OO335_003987"
                     /pseudo
     CDS             complement(2706..2855)
                     /locus_tag="OO335_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2931..4628
                     /gene="ospD3"
                     /locus_tag="OO335_003988"
     CDS             2931..4628
                     /gene="ospD3"
                     /locus_tag="OO335_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR6004161.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1660 a          861 c          887 g         1361 t
ORIGIN      
        1 tttagctgaa cgggccatat aaccacctga aagacaatga cattgcctgt ttttataacg
       61 gtaattgcag accatgacaa accgcagccg tcaggctgcc tactcgagtc acaaggctct
      121 ttgtgtccga tatgccggca gcgcattacc catgaaactg gctggaacgt acaccataag
      181 gtgaagaaag ttatgggggc ggtgaagaat tatctaacct tgtcttgtta catccaaatt
      241 gccatcgaca gttacacagt tgtgaagccg gctctctcaa aaagggctta taaaggcttg
      301 agcggtgtac cgggaaaccg gtatgcaccg ttcttagggg agggcagtcc agcaatgaac
      361 tgtccttacc cgacaaacat ccaaaacgaa cggaacgtgc tggaatccgc atataacccg
      421 ttgtaaagtg aggaaatgga cacctcactt gctcatgaga acgcccgcct gcgggcactg
      481 ttgcagacgc aacaggacac tatccaccag atggctgaat acaaccgcct gctctcacag
      541 cggatggcgg cttatgcttc cgaaatcaac cgactgaagg cgctggttgc gaaactgcag
      601 cgtatgcagt tcggtaaaag ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca
      661 ttttcacggg cgatctacgc cacacaggct ctgggttgca gtgactgctc cattaaggcg
      721 attttgaact cataagggaa gtaacaaaaa aactgcaaaa gtacaataat tatatatggc
      781 caaactaacc tctattaaaa aacatcagtt tttcacactt agttgagctt catgaaaaaa
      841 tcatcgagga tttcattgca gaatacacat tccactaact ggaagcactc cataagagga
      901 atcagagaaa gtatcgtttt tttatagtaa aattcattgc tgttcaataa aattgatata
      961 gttattaaat tatgaaaata atttcacaaa aaagattaac ttgagttctt aagcatcgga
     1021 ttttagatat tttgaaatat ctggcatcac ataacatcaa aagtatataa tactcttatc
     1081 taattacatg cagtatattg tatatatact caaaaattgt aattcatacc ctgctatatc
     1141 ttgtagggaa agatattaat taaaactgtt ttcatataag gttcatttta tgaatatatc
     1201 agaaacactg aactcagcaa atacccaatg caatatagat tctatggata acagattaca
     1261 tacattgttt ccaaaagtga catcagtgcg aaacgctgca caacaaacta tgccagatga
     1321 aaaaaattta aaagatagtg caaatattat taaaagtttc tttaggaaaa ctatagcagc
     1381 acagagttat agtagaatgt tctctcaagg ctctaacttt aaatctttaa atatagcaat
     1441 tgatgcacca tcagacgcta aagcctcatt taaggctatt gagcaccttg acagattatc
     1501 gaagcattat atatctgaaa taagggaaaa acttcatcct ctttctgcag aggaactcaa
     1561 tttgctttcg ctaattatta attctgattt aatcttcaga catcaaagta attctgattt
     1621 gtctgataaa attttaaaca ttaagtcatt caataaaatt cagtctgaag gaatatgcac
     1681 aaaacgaaac acatacgctg atgatataaa aaaaatagct aatcatgact ttgtgttttt
     1741 tggcgttgaa atctctaacc atcagaaaaa acaccccctg aatacaaaac atcacactgt
     1801 tgattttggt gcaaatgcgt atatcattga tcatgactct ccatatggat atatgacatt
     1861 aaccgatcac tttgataatg ctattccacc tgttttttac catgagcacc aatcattttt
     1921 agataaattt tcagaggtta ataaagaagt tagtcgatac gtacatggaa gtaaaggaat
     1981 tatagatgta ccaatattca atactaaaga tatgaagtta gggctcggat tatacctgat
     2041 tgactttatt agaaaaagtg aagaccaaag cttcaaggag ttttgctatg gaaaaaatct
     2101 tgcccctgtg gatctggata gaatcataaa ctttgttttt cagccagagt accatatacc
     2161 taggatggta agtacagaaa acttcaaaaa agttaagatt agagaaatat ccttagagga
     2221 ggctgttaca gcatctaatt acgaagaaat taacaagcag gtcactaaca aaaaaattgc
     2281 tctccaggct ctttttcttt cgattactaa tcaaaaagag gatgtcgcct tatatatatt
     2341 atctaatttt gagataacta gacaagatgt tatttccata aagcatgagt tgtatgatat
     2401 tgagtatcta cttagcgctc ataattcaag ctgtaaagta cttgagtatt ttatcaataa
     2461 gggattggtt gatgtaaaca caaagttcaa aaaaactaat agtggggatt gtatgttgga
     2521 taacgcaata aaatatgaga atgcagaaat gataaaacta ttattgaaat atggtgcaac
     2581 atctgacaat aaatatattt aatcaaaatt gaatatcgtt tagcaaaagg cagtatttgc
     2641 acaaaatatc gatatattgt ttattgtcag tatggctcaa tgtgataata gttggaaagt
     2701 ttgatgggtt tcgccccgtt gtagcggtag tcgaactgga aggtcttcag gcactgctta
     2761 cagcgataga gcagcccccc tgaactggaa tggccgttcc ggataccccc ctgagtttca
     2821 gagtaacggt gacaaaccac atcaatcttt gccatcaatc atccaaaggg caaagagtac
     2881 aacaacacta agtctgcgtc acaacccatc aatgaaagga atatatacat atgccatcag
     2941 taaatttaat cccatcaagg aaaatatgtt tgcaaaatat gataaataaa gacaacgtct
     3001 ctgttgagac aatccagtct ctattgcact caaaacaatt gccatatttt tctgacaaga
     3061 ggagtttttt attaaatcta aattgccaag ttaccgatca ctctggaaga cttattgtct
     3121 gtcgacattt agcttcctac tggatagcac agtttaacaa aagtagtggt cacgtggatt
     3181 atcatcactt tgcttttccg gatgaaatta aaaattatgt ttcagtgagt gaagaagaaa
     3241 aggctattaa tgtgcctgct attatttatt ttgttgaaaa cggttcatgg ggagatatta
     3301 ttttttatat tttcaatgaa atgatttttc attccgaaaa aagcagagca ctagaaataa
     3361 gtacatcaaa tcacaatatg gcattaggct tgaagattaa agaaactaaa aatggggggg
     3421 attttgtcat tcagctttat gatcccaacc atacagcaac tcatttacga gcagagttta
     3481 acaaatttaa cttagctaaa ataaaaaaac tgactgtaga taattttctt gatgaaaaac
     3541 atcagaaatg ttatggtctt atatccgacg gtatgtctat atttgtggac agacatactc
     3601 caacaagcat gtcctccata atcagatggc ctgataattt acttcacccc aaagttattt
     3661 atcacgcgat gcgtatggga ttgactgagc taatccaaaa agtaacaaga gtcgtacaac
     3721 tatctgacct ttcagacaat acgttagaat tacttttggc agccaaaaat gacgatggtt
     3781 tgtcaggatt gcttttagct ttacaaaatg ggcattcaga tacaatctta gcatacggag
     3841 aactcttgga aacttctgga cttaaccttg ataaaacggt agaactacta actgcggaag
     3901 gaatgggagg acgaatatcg ggtttatccc aagcacttca aaatgggcat gcagaaacta
     3961 tcaaaacata cggaaggctt ctcaagaaga gagcaataaa tatcgaatac aataagctga
     4021 aaaatttgct gaccgcttat tattatgatg aagtacacag acagataccc ggactaatgt
     4081 ttgctcttca aaatggacat gcagatgcta tacgcgcata cggtgagctc attcttagcc
     4141 cccctctcct caactcagag gatattgtaa atttgctggc ctcaaggaga tatgacaatg
     4201 ttcccggact tctgttagca ttgaataatg gacaggctga tgcaatctta gcttatggtg
     4261 atatcttgaa tgaggcaaaa cttaacttgg ataaaaaagc agagctgtta gaagcgaaag
     4321 attctaatgg tttatctgga ttgtttgtag ccttgcataa tggatgtgta gaaacaatta
     4381 ttgcttatgg gaaaatactt cacactgcag accttactcc acatcaggca tcaaaattac
     4441 tggcagcaga aggcccaaat ggggtatctg gattaattat agcttttcaa aataggaatt
     4501 ttgaggcaat aaaaacttat atggaaataa taaaaaatga aaatattaca cctgaagaaa
     4561 tagcagaaca cttggacaaa aaaaatggaa gtgattttct agaaattatg aagaatataa
     4621 aaagctgaat attatctcgt ggtgatacac tgaatttgag gtaaccggct catttaaacc
     4681 gtctggtctg ttacctccgg ttccacaaaa ataatgtcca tcattttgaa gtggacacta
     4741 tcgtatggaa taccggacct ggatcactg
//
LOCUS       DAJTSF010000193         4612 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000193 DAJTSF010000000
VERSION     DAJTSF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.197"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..332)
                     /locus_tag="OO335_003989"
     CDS             complement(<1..332)
                     /locus_tag="OO335_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6004162.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVING"
     gene            complement(337..1059)
                     /locus_tag="OO335_003990"
                     /pseudo
     CDS             complement(337..1059)
                     /locus_tag="OO335_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(1079..1801)
                     /locus_tag="OO335_003991"
     CDS             complement(1079..1801)
                     /locus_tag="OO335_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6004163.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(1861..3015)
                     /gene="yfcU"
                     /locus_tag="OO335_003992"
                     /pseudo
     CDS             complement(1861..3015)
                     /gene="yfcU"
                     /locus_tag="OO335_003992"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(2964..3719)
                     /locus_tag="OO335_003993"
     CDS             complement(2964..3719)
                     /locus_tag="OO335_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6004164.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(3801..4364)
                     /locus_tag="OO335_003994"
     CDS             complement(3801..4364)
                     /locus_tag="OO335_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6004165.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT         1148 a         1169 c         1068 g         1227 t
ORIGIN      
        1 ccgttaatca cgaccgttgt cgcctgcatc tgtagacgca ggtcatccat tgccagattg
       61 ttacaaacaa gcgagtagtc aatgggacgt ttataatcaa ccctattgat cttattaatg
      121 aatacattac caaactcaac tgccgcacca tttaccgtac aaggaggagg ggcatcgaca
      181 agcacccgta aattcatact tactgactta ctcgctgcaa ctgcaaagcc gctaccaaca
      241 caacatatca gtaacccagt taccgcccag acagaacaag atcctttcat actggctctc
      301 ccggcctcag cctttgcggc tctgctcttc atcgacctag caactgttac cgttgcaagt
      361 gaaaaagagc ggcatccgag cgccatagtc gttgacatag gtcagtaccg gcacgctacc
      421 catcttcaca ttcagcggtg cagaggtttt cggcgacatc actattggcg agaaaccggc
      481 tgccggattg ccatttttct gtgtgctggc attactgaaa atgacgtaat acgacgtcgg
      541 gttattgacg gtaaattcct gaccgttgcg ggttaaagta atcttatatt gctaggggtt
      601 tttcatgctg acgttctcca gcgccttcgg tcgccagaac aacttaatac gcgtttgtag
      661 tgctatttgc agcgtgttgg ctttgtttga ttttggcggg atctcacgca cgttgaagta
      721 gaacaggctt tcacgatcgg caggcaattt gttgatatcc ggcattccct gcacttttac
      781 ctgcccgttc atcattgaat cgatgcgttg caccggtggc agaacagtta acggtgaact
      841 gatcttgtta cccttttcat cttcgatcca gctttgggcc agataaggca gtttcggatc
      901 gttattgcgc aacgtcacgc tgattgattt gtcgctttca ttaaatatca aacgggtacg
      961 atctggcgtt acagaagcct gggcagacaa actggcagcg ctaagcagta acgctagcgt
     1021 cgtagcaccc aatttggcag agcaaagtaa atcagacatg gatcattcct taatcacgtt
     1081 attggttagc tgaaacagaa acagggggat tggtgggtgt cacctgttgc gtctgctcct
     1141 gaatgacagg ctttatgtca tcaggaacaa ccggcgcgac gttgccttta tgctggcatg
     1201 gcagtaacaa gccgttaaac agatcggcag gcagcgggtc cggcaggttg atatcgcaat
     1261 gcgcaacacc actccagaac acactcatgt gttcgccagg tttcactcca gccagataaa
     1321 cattgccgtc atcatcgaca aggcccactg tctgctcgtt atcatttttt acttctgcgc
     1381 caaacggagg atggctgccg tcttgtaaac gcagcaccgc catcgctttt tgaccactga
     1441 tgacggcaaa tttgcggtag ccaatcgccc cttcagttag cgtggcttgt accaccgact
     1501 gggttgcttc ggcgttttcc ggcagtttgt tgaggtcgat atacgcctga ttgcggtaat
     1561 agttattgac gtcagaaacg acggctttac caaacatatt ggtataaaca gcagcaccgt
     1621 taccttctac cggaacatcg gcaacgccat cggcatcaat caacaagcgt gtcccgccca
     1681 tattctgggt acggtgaagt gcgccaccgt gggcagtaag cgttgcgccg ccctgtaacg
     1741 acaagcccgc agaggtgtat tgcccttcat ggtagttcgc actgaggtca acctgcgcca
     1801 gcgaaccatc atggctgtaa tagccgtcaa cgctggtgtg tttgtcactg gtgccgacgt
     1861 tcaactgata gtgagtcgcg tcatcgacac ggctgaaata accgacctga ctgctgtccg
     1921 tcccactgcc atagtaccgt tatagctaac ggtactgttg tcgccccacg gcatactgag
     1981 cgaaatgtac atgcctttgt cggcctggtt gtcatactcg taacggtagc cagtcatcga
     2041 gatgctgaca ttacgaatac tgcccatatt gaaatagtgc gagagcatga tgttgtagtt
     2101 tgtctgctcc tcgcgatccc agtaggtatg gcgggtatag ttgagataaa ccgaaacacc
     2161 cgcatcgcgg aagttctggt tataagtggc ggtgtacatc tctttgtcgt tgcccgtgcg
     2221 taccattccg ctgtcactgg catccagata ctcgctcatg gtcataaagt tctcttccga
     2281 gaagcgatat ccagcaaaag taacgcggct gttgagctgg tcaaagtctt tggaataact
     2341 cacacggaag gagttaccgt ccagcgaacc tttgccataa gcggtgtctt tatccagttt
     2401 ggtgtgcgag tgagtaacat caaacgcgac cgcgccgaat gtagacaaat cgcgaccgac
     2461 accaagcgcc gcagactgat agttttcatc tcccagtgcg ccgccatata acgaccagcc
     2521 gttagcgatc ccccaggaag cttcagcacc agaaaaaaat tcaccctcga catggtgtcc
     2581 ccactcttgc ggacggccca tcatgatctt atagcgaacc tgacctggac gagtgaggta
     2641 tggcatcgag gcggtgctga tgtcatattc ctgcacctgg ccgttctgtt cttcaatgcg
     2701 aatatgcaac gtaccggaga cagaatcacc aagatcctga atacgaaacg gtccagccgg
     2761 aacctgcgtt tcgtaaatca cacgccccat ctggctgacg gtcacttttg ctgtggtgtg
     2821 tgccacgccg gaaatgtctg gcgcgtagcc gcgcagattg ggaggcaaca tttgatcgtc
     2881 agtactgaca ctgccaccaa catagttaaa accatcaaaa atatcggatc tgaggtaatc
     2941 ctcgcccagc gccagtttgg cttttaatga tggtaacgcc cgcctggcat agtagcgact
     3001 ccactcccat ttttttgagt ttcatcgccg ctgaattcat cgtcatcatt actgcgagta
     3061 tgttgatagt tagtctgcca gtcagcacgc atacgccacg gccctaggtt aaccccaacc
     3121 gtcccgttgc cgctgatctc gttactatca tcaccgccat tttcttcgtg ccgggtttgt
     3181 gcgttgatgc tgtagtccgc gacgatcccg gagatgccgt catcccaacg tgaaggcgga
     3241 tccctgtcgg gataagtata ttcgaggtaa gcctgtggca gtgaaatgac taatgcggac
     3301 tggcttaaat cagccttaat ttccatgcct tccagttgac cggatttcag gcatttagca
     3361 tcgtggctcc attgcagatt tttcgccacg tcttctttta aaccaaactg cgctaccagt
     3421 tccggtgtca gacaagcata gcttttgctc gcgtcatctt caccagcata ccagtaaata
     3481 tcgtactctt ccgccaatgg ctgtttattt agttgaacct gtaaattgta ttttccgggc
     3541 tcaacatatc cctggctgga aaaacgcttc atatcaattt ttgtgtcgcc ttttaattcc
     3601 agaaaacggg aatcaaactg aatgtcgtct tcagcccaga cactgctata actccctgac
     3661 attgccagcg caatgcacca cggaagaatc cgtaatcgaa aaagagaatg gtcaggcata
     3721 cacgtttcta tccgttttta ttcttgttcg gtcaaaatac atttaaatcc cccatagcag
     3781 tgtcgctatg ggggtatgcc ttacaggtaa gtaatctgga aggtggtatt tgcttcaaaa
     3841 ttacccagat ctggcgcatc agctgcgccg accagccagg cgttaaagtt cagtgtctgc
     3901 ttcgctttgc ctttgttggt agaagtgtct ttcactattt tctggtcaat acccatgccg
     3961 cttttgtaag aggtacccag agagtcaccg atcgccaggc taacattgtt aaatgccgca
     4021 ccggtatcgg tattgaaaat ggtgtaataa ttgccgctat ttgcagaaga aacggtacca
     4081 gtaaaggtag tggtcattgt ttcctgagta tcaaatacgc aatcttgcag acgaatctgg
     4141 aaacttttcg gcgtggtggt gccgttattt ttcagttttt cggcaccgat atcacccata
     4201 tcaacttcca gtttgtgatc gtccggaacg atagaacaca ctgagtcttc aatggtgccg
     4261 taaaaacgtg cagtaccgtt gttacccgca gcgattgttg ccgtagaggt gaacacgccc
     4321 atcaccattg ccgcagcaat agctgtttta acaaacttac tcattatttg tccttaaact
     4381 ataaataatt aaaaatataa acattgcaat acattgattc agtcaatagc caatgtttat
     4441 acccagccat tgaatacggc atgcgaccca aaaagaacag gtcgtagaaa attgtattca
     4501 ttggtgtggg gccgcatagt gatgtttcca cacccctgat ccaatttaat tttactaaag
     4561 agtaaatgta gatttaatta atatattgac agaagaaagg ttatcttaag ga
//
LOCUS       DAJTSF010000194         4602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000194 DAJTSF010000000
VERSION     DAJTSF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.198"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(78..866)
                     /gene="phoH"
                     /locus_tag="OO335_003995"
     CDS             complement(78..866)
                     /gene="phoH"
                     /locus_tag="OO335_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR6004166.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="OO335_003996"
     CDS             complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="OO335_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR6004167.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="OO335_003997"
     CDS             complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="OO335_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR6004168.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3929..>4602)
                     /locus_tag="OO335_003998"
     CDS             complement(3929..>4602)
                     /locus_tag="OO335_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR6004169.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1116 t
ORIGIN      
        1 ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct
       61 ctgcaccgat aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt
      121 acgcaatcct cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc
      181 agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg
      241 ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca
      301 gtcacattct gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata
      361 taggcgaacg gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag
      421 gaagccccta agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt
      481 tctgcgatat caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg
      541 acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc
      601 caggtttttc cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc
      661 tgcagatagt gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg
      721 cgggccatac caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct
      781 tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct
      841 ttgatcactg ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac
      901 gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca
      961 tagtttgctt ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta
     1021 acacgagtaa agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga
     1081 aatctccgcg agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca
     1141 tccgcgatct ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag
     1201 tacaacagaa ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca
     1261 tttcggtgac attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc
     1321 tgtgtgcttt ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg
     1381 gcgcgaacgc cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg
     1441 ataaacctgc gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga
     1501 taatcgttcg cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta
     1561 acgtattcct ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc
     1621 agatcgtgtt ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc
     1681 cccaacgaat aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg
     1741 ggattcgcca gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg
     1801 taatcaggca catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg
     1861 ccaaaaatcg tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga
     1921 atcaagcgta ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc
     1981 gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg
     2041 tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg
     2101 gaaataaacc cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata
     2161 tcgcgcagcg catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca
     2221 ccatgacata acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt
     2281 tttggcatct gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac
     2341 gtgatggtga gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc
     2401 aggcgcggat ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga
     2461 gtcaacaagc gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc
     2521 accagcatca ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac
     2581 ggctgtttct cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt
     2641 gcatgagcga ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt
     2701 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
     2761 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc
     2821 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
     2881 gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt
     2941 tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga
     3001 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca
     3061 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
     3121 tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc
     3181 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
     3241 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
     3301 agaacagttc agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg
     3361 ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca
     3421 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
     3481 ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc
     3541 ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg
     3601 tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt
     3661 tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc
     3721 cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct
     3781 gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact
     3841 gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt
     3901 aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc
     3961 gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt
     4021 cgctgacgct cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg
     4081 agtgagttga gaggaccgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc
     4141 cggcttcatg aaatgcgcga atggcaccag ccgccagccc tgcggcgacg aagagaataa
     4201 acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca
     4261 ggaagccgag caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc
     4321 cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa
     4381 cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc
     4441 tatcgactgc ctgttccagt tgcactttga cgttgcgcga cactttgcgc atccagaaaa
     4501 ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgct
     4561 ctttttgcgg aaattcgccg gtggtttcgt taatgaagat cc
//
LOCUS       DAJTSF010000195         4530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000195 DAJTSF010000000
VERSION     DAJTSF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.199"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1222
                     /locus_tag="OO335_003999"
     CDS             <1..1222
                     /locus_tag="OO335_003999"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR6004170.1"
                     /translation="PYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLYP
                     LSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGISN
                     YAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGVT
                     NIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEKW
                     FIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILPV
                     IVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHLL
                     IANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRAA
                     RVFVNDERFTRGGN"
     gene            1222..1956
                     /locus_tag="OO335_004000"
     CDS             1222..1956
                     /locus_tag="OO335_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6004171.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2423..3184
                     /locus_tag="OO335_004001"
     CDS             2423..3184
                     /locus_tag="OO335_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6004172.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3228..4307
                     /locus_tag="OO335_004002"
     CDS             3228..4307
                     /locus_tag="OO335_004002"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6004173.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4304..>4530
                     /locus_tag="OO335_004003"
     CDS             4304..>4530
                     /locus_tag="OO335_004003"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6004174.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSI"
BASE COUNT         1243 a          862 c         1117 g         1308 t
ORIGIN      
        1 tccgtatggc attttccagc agcgaaatgt tcttgcgagc tggctggcaa caggttgcgg
       61 cgtagcgttg tatctggctt taacggcgcg tactcgttct ctgacgttga tatgtttgtt
      121 gtcgttatac cctctgtcta cagctttagt ccttacacaa agccgtgtcg gccttcttgg
      181 tttattttgc gtcatcgtgc tggccgctct cgcggacaga ccccgctggc gcggacgtcc
      241 ccttagcggt ctgttgcgct tgatcttcgt cgcctccttg ctgtgctggt gtgtcggtat
      301 ctcaaactat gccatgccag gggagacaac ggctgacttt acgcattcat catcgaatga
      361 ccagcgtata cgtattctga aaggtgccat aagtcttatt caacagcgcc ccttaaccgg
      421 ttgggggtta ggcagctttg aagcgacctt cccgcaagcc ttagaggatg tgggcgtgac
      481 aaacatagag agtgatacgg ctacgcatcc ccataacgaa ctgctgttcg taacggctga
      541 aggtggcatc gtcgccttgt ttggtttttt gctgctatca ggcgtttggc tgtggccgtg
      601 tatatgccgc ctgaaaatga caaacaataa cgcgcaccat caatgtgaaa agtggtttat
      661 tgccttaatg gggttgcctt tagtcattca tatgatgacc gaatatcctc tctatacgtc
      721 tgttccccat ttgatgctgt tgctgctcct tttccgcatc ggaatgccgg aaaacgtgat
      781 gaatcaggta cgtataccta aaacagtccg gttgggtatt ctgcctgtca ttgtgctcat
      841 tatgaccgcc gcgctggtca tcctggtagc gggatttgat gcacagcggg aactgaccag
      901 agctgaagct gatatgaacg aaggatttat ccctcgcctt ccggaacata gctggaaata
      961 cctgacgcag gcagaacgtc ttgagcgcga tcagcatctg cttattgcga ataaaccggg
     1021 gttcgttcgc gatacggaaa gcatgtctct tttcactgtg tggggaaata aatggttggc
     1081 aatccacaat gatgcagacg taagcgctgc aatgatattc attgcgcaca ggcgcggtga
     1141 ttatctgaca gctgaaaaaa taagaaaacg cgcagctcgg gtatttgtaa atgacgaacg
     1201 ttttactcgc gggggaaact aatggttaac gatctgacgt ataagaaatt agaaagcaca
     1261 aacatcaact gtaatattct ctcgccagaa aataccacat ggacaaatgt cacggtgatt
     1321 ggcctggatc tttttgccat ttgcggcgtg tgcagtttgc tcaagcgtgt tgggggagtg
     1381 aaacttcagg ttttcacttc cttacccact gatatgaatg ttctgactga cagtagcatc
     1441 gtgatatggg taaaaatgcg tcacgatggt ttgcctgaat tagccggaca tattgtgaag
     1501 gtttgtcgtc gataccgtct gatgcggcaa ctcgttatta gcgatgctat tcctgaagga
     1561 atgacgaatg atatcgggcc tctttcccga gtatggctta ctcatgggag tgcaaaagtg
     1621 gatattttat tatcttcatt acggaaggtg ttaaatgctc attgtttgcc tggtcctttg
     1681 tttataaaac gcctgggaag agtgcaatgg agggttcttt tactccgcgc gaaggggatt
     1741 agcacacgta aaattgcaga aatctgtggt attggcctca agaccgttag cgcccatgag
     1801 tccgcaattc gagaacgatt aggcaccagg aataaaagtg agtacgtctg gttagtacgt
     1861 aatgctcaac ttatacaaca ggcgataccc gcgttgggaa gagatgcgtc aaggtatgaa
     1921 agatgtaaca atatgcgaac aaagcaaaaa gtataattga tgcgtcatag ataactctct
     1981 ttttcgatga cgaaatcgct gagcatgcgt ggtgaggtta gtaatagatg ggtgctatgt
     2041 tttgccacgg gtttgtagct gtgaaacctg gaaaaaaata agaattaaaa atatttaaag
     2101 agagttaata aggttctggt tgtaattgtc ttggtcaaaa aaaagatatt gtcaatgttg
     2161 ttttttaaat tttcaggttt tggttaaata tttgcttgca aatataggtg attttttatt
     2221 gtaggctttt gaaggctatt ataactgtgc gctaaattaa tgcgttgaat aaaagaaata
     2281 acaaaattaa gaaaagccat atttacgata tgggcaacat aaatataatc taaaaaagag
     2341 taaggcgttg taggttggtt ccgcgttcat taccttgtta gtgaacgcgg atttatcagt
     2401 tgtcgaaatg gtttttataa taatgaaaaa ttttaatgta ctaaccaaag tcgtattttg
     2461 cttgtcgttt ttatcaagcg catttcccac ctatgctgcg gatgatgtca aagtgactca
     2521 aaacacttca gtttttaaac tgaagcttag tgcaacgcga ttaatctatg aacaagggtc
     2581 ggatggcgca gcgataactg tgacaaatcc gcaggattgg ccgatgcttg tgcagagtaa
     2641 agtctttgaa gaagataaag taactgcagc gccctttatt gtcactccgc cactgttcag
     2701 gcttaatcca ggccagcaaa ataaaatgtg ggttgtgcag actgcgccag ctttacaaga
     2761 cagggaaaaa ttacactggc tatgcgccac cggcattacg cctaaaaaga gtgatgtttg
     2821 ggccgagaaa gataaagaaa aaattgctga aaatgtgaac ctgaacatcc aggttaacgt
     2881 acatacctgc ataaaactta ttaccaggcc atctggtatt gaaggcaaga tggttaatgc
     2941 gagtgagaag ttatcgtgga aaaagcaggg tgataaaatt gtcgcggtca atcagtcgcc
     3001 gttctacatc aacctttctt ctttacaggt tggcggtagc aagattgaga aactagatta
     3061 tattgcgccg ttttcgcaac atgagtttgt tatgccaaaa actagcggcc aggatattaa
     3121 atggtctgtg attactgatg aaggtggtga gagtcgtttg tttatatctc atttgcaatc
     3181 ataatgtatt acatttttat tgaatatttt tatgccatgc atattccatg ttttatcgac
     3241 tgtcattaat tttgataatg gccttacttg cgggtcagtt aagtgcccag gagtggtcat
     3301 ttgattcttc acagttggaa ggaaacgtta gtgcggacac tgtggcaatg tttaatcagg
     3361 gagagcaact ccccggaaat tatcgtgtgg aaatttatct taacggtgaa aaagttgatg
     3421 ttggggagtt tccctttcat cgtccggaat cccctgaaga aaaagagctt gttccatgtt
     3481 taaccgttga tgaccttatc cactatggca ttaaaattga taaaagcagc agtgatactg
     3541 acaataaaaa gaatcagtgt tttaaatgga actctattga aggtttgaaa gtaaattatg
     3601 attttgatag ccagcgcgta cagataaccg ttccacaact gtatctgcaa gataaaaaaa
     3661 gtagtttagc accagtgtcg ttatggaatg agggtgtagc tgcattccgc atggtgtatc
     3721 agacaaatat tgatatttcc aaacaaaatg ataatcaatc taccacgcgt aattctcgtt
     3781 atggcaggtt cactccaggg tttaatcttg gtgcctggag gtttcgtagt tcagtgacat
     3841 ggagcaagga acttggccag tcagaaagat ggcaaagggg ttacatgtgg tttgagcgag
     3901 gcataaatgc gattaaaagc cgattaacgc ttggagaatc ttatacgtcg tctgaggtgt
     3961 ttgacagcat accttttcgc ggcgggatgt tagcgaccga cgatgcgatg actccgccgg
     4021 aggacagcta ctacacacct gtggtgcatg gaatagcaca aagtgaggcg caggttatta
     4081 ttaaacaaaa tgggcaaatt atttttaccc gttctgtgcc gccagggcct tttgcgctgg
     4141 ataatttacc aacgctggcc gttggcgggg agcttgatgt tacagtgaga gagagtaatg
     4201 gcgaggagca gtatttttcc gtgccatttc aaacgccggc catcgctctg catgaaggat
     4261 atttcaaata tagtgtgatg ggggggaata taaagaagaa ggtataaaaa gctcccctgt
     4321 ctccgtgggc gaaatcacgg cgatgtacgg gcttccttat ggcgtcacgg tgtatggagg
     4381 gattcaaaac gccactcatt ttaatgcaat atctacgggt attggcattt ctctggggct
     4441 gttagggagt ttatcgacag atattacgcg ttcgattgcg aatttatatt atgggaataa
     4501 atatcgtatt cgttatagta aatccattag
//
LOCUS       DAJTSF010000196         4364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000196 DAJTSF010000000
VERSION     DAJTSF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.200"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(249..995)
                     /gene="cutC"
                     /locus_tag="OO335_004004"
     CDS             complement(249..995)
                     /gene="cutC"
                     /locus_tag="OO335_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6004175.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(1009..1575)
                     /locus_tag="OO335_004005"
     CDS             complement(1009..1575)
                     /locus_tag="OO335_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6004176.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1791..3524
                     /gene="argS"
                     /locus_tag="OO335_004006"
     CDS             1791..3524
                     /gene="argS"
                     /locus_tag="OO335_004006"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6004177.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            3701..4189
                     /locus_tag="OO335_004007"
     CDS             3701..4189
                     /locus_tag="OO335_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR6004178.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         1162 a         1072 c         1051 g         1079 t
ORIGIN      
        1 ttgctaagga ataacccagt tgctatttag aatatatatt tatcagtttt agcaatttaa
       61 atcccataat caatgtgaat ataaaaaata tttttagcac aacgctatgc gcactgtcac
      121 ccgctcaaca gatcacggtt atcgttcgtt ttttatactg ctcagggata aaaaaaggcc
      181 cctgttgaaa ttgcaggggc ctggtacgag caagcatcat attgggcgac atgatgcaac
      241 ggtaaaaatc atttggcctg atggcgttca atgattcctt tcatttcagc aaccgccgcc
      301 ccgtctacga tataacgcga atactcgtcc gcgtgttcat ctgatgacat ggacaatcct
      361 tgattacgat aacgcatcgg tgaggcttgc cacgctcccg cggagctatg gacttccagc
      421 actccggcat cgaggaagtg gtgcaggttt tctgcacgga ctcctgctcc agccataatg
      481 attggagcat cacgatgggc aataagttcc ataatttttg ataaaccttg cagtgcgtct
      541 gatttttgcc ctgatgtcag tacccgggca atgcccaatt ccgcaagatt attgagtgta
      601 tataaagggt tagcgcacat atcgaaggcg cgatgaaaag tcactgccag cggaccggca
      661 gcagccatta ttttttccat tcgtggcata tcgacattcc cgtcaacatc gagaacgccc
      721 gtcaccagtc caggaaaacc taattcgcgg accgtgcgca catcctcaag aatggcggca
      781 aactcaccgt cgctgtagca aaaatcacca ccgcgtgggc gaattatcgg atgcacaggg
      841 atcgtcaccc gctggcgcac ggatttcagt acccccaacg acggcgttaa gcccccctct
      901 tttggggctg cgcataattc aactctgtct gcgccgtttt gctgcgccgt tagtgcacat
      961 tccatgctgt aacagcaaat ttccagtaat gccatttttg ctccttaatt acgctgactg
     1021 ctcgctggca acgatctctt caatggacca cggatgaaac ttaatagttg ttttgccatc
     1081 ggtgaccgcc agcgtcggat tcggtaatcg ctcatgctca ccttttggtg aactggtttt
     1141 aactgagatc cccggtaggc tgagtccttc atcagaaagc aatgccagcg cacgggcatt
     1201 cagcgtttct ggatcacccg gcagaacgat ttcaatatgt tcccaacctt cgtgtgggta
     1261 acgtttttcc ccgggccacg gtagctccac attggaaaac tgccagtgcg caacctgtac
     1321 cggttcatgc aatttaaaca gacaaatcgg tctgccattg atcatttttt ctgacaaaag
     1381 ctcgccacac tgttcaaaac cgcgacgcca gcgctcagcg gtgacgtttt gatggcaacg
     1441 caaagaaatg tgatcggcag tcagcggagt gatatccaac cccagacggc gggaaagttc
     1501 atctaatgcg tgggtaaatc gcggtaaatc cgatgcaata tcctgcagct cgtcgataga
     1561 ttgccagttc gccataatca ctcttcgtct ttcagtaaaa gcgttaattt accctgttgc
     1621 cctgtgccaa ccaaccgctg atttcacgcc gcttctgatg caatagtgaa aacggcaata
     1681 cgccacgcgc acgttgctga cgaaaacagc catttgcagt atactcccgc cctaatttct
     1741 ttaactggtg cgggcaattt ttgctcgctt catcaatgta aggtattccg gtgaatattc
     1801 aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc ggcaggcgcg cctgcggatt
     1861 gcgaaccgca gattcgtcag tcagcaaaag ttcagttcgg cgactatcag gctaacggca
     1921 tgatggcagt tgctaaaaaa ctgggtatgg caccgcgaca attagcagag caggtgctga
     1981 ctcatctgga tcttaacggt atcgccagca aagttgagat cgccggtcca ggctttatca
     2041 acattttcct tgatccggca ttcctggctg aacatgttca gcaggcgctg gcgtccgatc
     2101 gtctcggtgt tgctacgcca gaaaaacaga ccattgtggt tgactactct gcgccaaacg
     2161 tggcgaaaga gatgcacgtc ggtcacctgc gctctaccat tattggtgac acagcagtgc
     2221 gtactctgga gttcctcggt cacaaagtga ttcgcgcaaa ccacgtcggc gactggggca
     2281 ctcagttcgg tatgctgatt gcatggctgg aaaagcagca gcaggaaaac gccggtgaaa
     2341 tggagctggc tgaccttgaa ggtttctacc gcgatgcgaa aaagcattac gacgaagatg
     2401 aagagtttgc cgagcgtgca cgtaactacg tggtaaaact gcaaagcggt gacgaatatt
     2461 tccgcgagat gtggcgcaaa ctggtcgaca tcaccatgac gcagaaccag atcacttacg
     2521 atcgtctcaa cgtgacgctg acccgtgatg acgtgatggg cgaaagcctc tacaacccaa
     2581 tgctgccagg aattgtggcg gatctcaaag ccaaaggtct ggcagtagaa agcgaagggg
     2641 cgaccgtcgt attccttgat gtgtttaaaa acaaggaagg cgaaccgatg ggcgtgatca
     2701 ttcagaagaa agatggcggc tatctctaca ccaccactga tatcgcctgt gcgaaatatc
     2761 gttatgaaac actgcatgcc gatcgcgtgc tgtattacat cgactcccgt cagcatcaac
     2821 acctgatgca ggcatgggcg atcgtccgta aagcaggcta tgtaccggaa tccgtaccgc
     2881 tggaacacca catgttcggc atgatgctgg gtaaagacgg caaaccgttc aaaacccgcg
     2941 cgggtggtac cgtgaaactg gcagacctgc tggatgaagc cctggagcgt gcacgtcgtc
     3001 tggtggcaga aaagaacccg gatatgccag ctgacgagct ggaaaaactg gctaacgcgg
     3061 ttggtattgg tgcggtgaaa tatgcggatc tctccaaaaa ccgcaccacg gactacatct
     3121 tcgactggga caacatgctg gcgtttgagg gtaataccgc gccatacatg cagtatgctt
     3181 acacgcgtgt attgtccgtg ttccgtaaag cagaaattaa cgaagagcaa ctggctgcag
     3241 ctccggtaat catccgtgaa gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg
     3301 aagaaaccct caccgtggtt gcccgtgaag gcacgccgca tgtaatgtgt gcttacctgt
     3361 acgatctggc cggtctgttc tctggcttct acgagcactg cccgatcctc agcgcagaaa
     3421 acgaagaagt gcgtaacagc cgtctgaaac tggcacaact gacagcgaag acgctgaagc
     3481 tgggtctgga tacgctgggt attgagactg tagagcgtat gtaatcgatt tttcgtgaga
     3541 gtgaagcctg atcagggtta gccgatcagg cttttttatt gccatctaaa tgtattctga
     3601 aaacggacat gccattgttt tctcactgtt gagtaagagg ccagaagcgt aatatccagg
     3661 cccaggaaaa cgataacggt tcaatttaag gaataccgaa gtgtttaaat ttcttgtatt
     3721 aacattaggc attatctctt gccaggctta cgcagaagat acggttatag taaacgacca
     3781 tgacacttca gccatcaaag attgttggca aaaaaattca gatgatgata ctgacattaa
     3841 cgtgatcaaa tcatgcctgc gacaagaata caatctcgtc gatgcgcaat taaataaagc
     3901 ctatggtgaa gcttatcgtt atatagaaca agtgccacgc acaggtgcaa aaaaacttga
     3961 tactgaacaa cttaacttgc ttaaaaaatc acagcgagcc tggctggatt ttagggacaa
     4021 agaatgtgaa ttaatccttt caaatgagga tgttcaggat ttaagtgacc cctattctga
     4081 atcagaatgg ctatcatgta tgatcataca gaccaatacg cgaactcgcc agttgcagct
     4141 ataccgtaac tctgaagatt tttatccaag ccctttgaca agaggataat tcacatcttt
     4201 ttggcatgtt ttgttgcaag ctattcctga taaatgattg caacaaaaca tccagccttt
     4261 tgactgagtt attaaacata ctcgcgagcg cgtaattttt tgtctttcag cgataattca
     4321 caatcacttc attccgttgt acctttagcg gtggaattaa ccgc
//
LOCUS       DAJTSF010000197         4231 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000197 DAJTSF010000000
VERSION     DAJTSF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.203"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..446
                     /locus_tag="OO335_004008"
     CDS             <1..446
                     /locus_tag="OO335_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6004179.1"
                     /translation="SACAPETGLQQLVATPVPDEQRISFWPQHFGLIPQWVTLEPRVF
                     GWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAG
                     IAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            462..938
                     /locus_tag="OO335_004009"
     CDS             462..938
                     /locus_tag="OO335_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR6004180.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            1001..1222
                     /locus_tag="OO335_004010"
     CDS             1001..1222
                     /locus_tag="OO335_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR6004181.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1385..1759
                     /locus_tag="OO335_004011"
     CDS             1385..1759
                     /locus_tag="OO335_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR6004182.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            1806..2180
                     /locus_tag="OO335_004012"
     CDS             1806..2180
                     /locus_tag="OO335_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR6004183.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            2177..2668
                     /locus_tag="OO335_004013"
     CDS             2177..2668
                     /locus_tag="OO335_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004184.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            2680..2877
                     /locus_tag="OO335_004014"
     CDS             2680..2877
                     /locus_tag="OO335_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004185.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            2962..3522
                     /locus_tag="OO335_004015"
                     /pseudo
     CDS             2962..3522
                     /locus_tag="OO335_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            3594..>4231
                     /locus_tag="OO335_004016"
     CDS             3594..>4231
                     /locus_tag="OO335_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR6004186.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRI"
BASE COUNT         1029 a         1150 c         1058 g          994 t
ORIGIN      
        1 cttcggcctg tgcaccggag actggcctgc aacaactggt tgccactccc gtccctgatg
       61 aacagcgcat cagcttctgg ccgcagcatt ttggcctcat tccacagtgg gtcaccctgg
      121 aaccccgtgt cttcggctgg atggaccgtc tgtgtgaaga ctactgcggt ggtatctgga
      181 atctgtacac cctgaacaac ggcggggcat ttatggcacc cgaaccggat gacgatgatg
      241 acgaaacatg ggtactgttc aatgccatga acggtaaccg cgctgaaatg agcccggaag
      301 ccgccggcat tgctgcctgt ctgatgacgt acagtcatca tgcctgtcgt acggagtgtt
      361 atgccatgac ggtccattat taccggctgc gggattacgc cctgcagcat ccggaatgca
      421 gcgccattat gcgcatcatc gactgaatgg aggcagggac aatgcagcag ctttcctttc
      481 tgcccggaaa catgacgccc ggcgagcgca gtctcattct gcgggccctg caaaccctgg
      541 accgccatct tcatgaaccc ggcgtggcct tcacctccac ccgtgcggca cgggaatggc
      601 tgattctgaa catggcggga ctggagcggg aagagttccg ggtgctgtat ctgaacaacc
      661 agaatcagct gattgccggt gaaaccctct tcaccggcac catcaaccgt acggaagttc
      721 atccccggga agtgattaaa cgcgccctgt accacaatgc cgctgccgtg gtgctggcgc
      781 acaatcaccc gtccggtgaa gtcacaccca gtaaggcaga ccggcttatc accgaacgtc
      841 tggtacaggc actgggcctg gtggatatcc gggtgccgga ccatctgata gtcggtggca
      901 accaggtttt ctcctttgcc gaacatggtc tgctttaacc cgtcacaacc acatcacacc
      961 tgttttcact tttatcttct gtcttcagag gtatcacatc atgagaatca tcacccgtgg
     1021 tgaagccatg cgtattcacc aacagcatcc tgcatcccgt ctttttccgt tctgtaccgg
     1081 taagtatcgc tggcacggca gtgctgaagc gtatactggt cgtgaagtgc aggatattcc
     1141 cggcgttctt gccgtgtttg cagaacgccg taaggacagt tttggcccgt atgtccggct
     1201 gatgagtgtc accctgaact gatgttccgt cacccgggaa acgagtacga ccagatgacc
     1261 tcagacgccg ctgtggatga ggccattctc atcaatgcat acatcttcac cgaaaccggt
     1321 cagcgcgccg gctgacctgt catcgttaaa ccattcttaa ccacttttca gagaggattt
     1381 tatcgtgtca gacaaactct ccgggataac tcatcccgat gacaatcacg accgcccctg
     1441 gtgggggctt ccctgcacag tgaggccctg ttttggcgct cgtctggtgc aagagggtaa
     1501 ccgcctgtat taccttgccg accgcgccgg tatcagaggc cgcttcagcg acgcagatgc
     1561 ataccatctg gaccaggcct ttccgctgct gatgaaacaa ctggaactca tgctcaccag
     1621 cggtgaactg aatccccgat atcagcatac cgtcacgctg tatgcaaaag ggctgacctg
     1681 cgaagccgac accctcggct cctgtggtta cgtttatctg gctgtttatc cgacaccagc
     1741 acccgcaacc acctcataac cccgaagctc tcacctttag cactaccagt tacagagaac
     1801 ttaagatgaa aacattatcc gacacacatg tacgggaggt atctcgctgc ccgtctcccg
     1861 tcaccatctg gcagacactg ctcatccgac tgctggacca gcattatggc ctcacactga
     1921 atgacacacc gtttgtcgat gaacgtgtga ttgagcagca tattgaggca ggcatttcac
     1981 tgtgtgatgc agttaatttt ctcgtggaaa aatacgcgct ggtgcgtacc gaccagccgg
     2041 gattcagcac ctgtccccgc tctcagttaa taaacagtat tgatatcctc cgggctcgcc
     2101 gggcgaccgg cctgatgacc cgcgacaatt acagaacggt aaataacatt accctgggta
     2161 agtatccgga ggcgaaatga tgaaactggc cctgacactg gaagccgaca gcgttaacgt
     2221 acaggcactg aacatagggc gcattgtcgt tgacgtcgat ggtattgagc tcgctgaact
     2281 gattaacatg gtctgcgata acggctactc ccttcgtgtt gttgatgaat ctgaccagac
     2341 ctcagcagaa tgcacgccac catttgctac ccttaccggc atacgctgca gtaccgcaca
     2401 tatcacggaa acggacaacg cctggttgta ctcgctgtca caccagacca gtgacttcgg
     2461 tgaatcagaa tggattcatt ttacaggtag cggatatctg ttacgtaccg atgcgtggtc
     2521 atacccggtt ctgcggctta aacgcctggg gctgtcaaaa acgttccgtc gtctggtcgt
     2581 cacactcatc cggcgttatg gcgtcagtct cattcatctg gatgccagcg ctgaatgcct
     2641 gccgggttta cccactttcg actggtaacc aggaacaaca tgaaatcatt aaccacggaa
     2701 acagcgctgg atattctgat tgcgtggctg caggacaata tcgactgcgg atcgggaatt
     2761 atctttgaca acgatgagga taaaacggat tcagcagcac tgttgccctg tatcgaacag
     2821 gcccgggagg atgtccgtac cctgcgtcat ctgcagcttc tgcaccagaa ccggtgaagc
     2881 tcactcacca gacttcattc ctctgacgcc agcctgcaca gggctggcgt tttcatttat
     2941 ctgcaaaaag gaatatcgat tatgtcagat atcacaatct cccgtccgga agtggtcaac
     3001 gggcatacgg acgttatctg ctcaaccagc attcgccaca ttctggctgt acgaaagagt
     3061 acactgttgc aaatcgacac acttatccga caactggctg aaatctcggt gctgacggaa
     3121 agcatcggag gtaaaactgc cccggactgg gccatgaaac aggattttcg ctgcggctgc
     3181 tggctgatgg agaaacccga aacagcaatg aaagccatta cccggaatct tgatcgcgaa
     3241 atctggcgtg acctgatgca acgttccggg atgctgtcat taatggatac gcaggcccgt
     3301 gatacatggt accggtcact ggagtacgat aattttccgg aaatcagcga agcgaacatt
     3361 ctgagcacat ttgaacaact gcaccagaat aaggatgagg tgtttgagcg aggggtgatc
     3421 aacgtcttca gagggctgaa ctggaattac aaaaccaatt gcccctgtaa atttggcagt
     3481 aaaattatcg tcaacaatct ggtgagatgg gaccggtggg gataatgata tggtcacccc
     3541 caccttaaca gcagtacgct gttaaggtaa atccctttaa cggagagacc atcatgcaga
     3601 acatcacact tattggtatc gatctcggaa agcactcctt ccacattcat tgtcaagaca
     3661 agtctggtaa ggctcttctg cgtaaaaaat ttacccgtac taaactgatg cagtttctgg
     3721 cgacatgtcc gccctcagtt gttgtaatgg aagcctgtgc gggtgcacac tttatggctc
     3781 gctgtatctc tgattttggg catgaaacta agctgatttc tccacagttc gttcgcccgt
     3841 ttgtcaaaag taataagaat gattttgttg atgcagaagc catatgcgaa gcggcttcac
     3901 gaccatcaat gcgttttgtc cagccaagaa ctgagaccca gcaggccatg agggcgttac
     3961 accgggtcag agaatcgctc atcagagata aggtgaaaac aaccaaccag atccacggtt
     4021 tcttgctgga atttggaatc agcctgccga taggtgatgc tgtaataaaa cggttgtctc
     4081 tcgtgttggc tgaacacgaa atccccgaat atctgagccg ccttttggtg aggttgcata
     4141 cccactactt ttaccttgtt gagcaaatag cagagctgga atcagaatta agtcagtcaa
     4201 tcaacgctga tgataccgct cagcgcatta t
//
LOCUS       DAJTSF010000198         4209 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000198 DAJTSF010000000
VERSION     DAJTSF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.204"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO335_004017"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO335_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6004187.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO335_004018"
     tRNA            complement(324..399)
                     /locus_tag="OO335_004018"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO335_004019"
     tRNA            complement(406..480)
                     /locus_tag="OO335_004019"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO335_004020"
     tRNA            complement(597..681)
                     /locus_tag="OO335_004020"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO335_004021"
     tRNA            complement(690..765)
                     /locus_tag="OO335_004021"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO335_004022"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO335_004022"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6004188.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO335_004023"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO335_004023"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6004189.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO335_004024"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO335_004024"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6004190.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAJTSF010000199         4198 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000199 DAJTSF010000000
VERSION     DAJTSF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.205"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            84..752
                     /locus_tag="OO335_004025"
     CDS             84..752
                     /locus_tag="OO335_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004191.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="OO335_004026"
     CDS             complement(995..1690)
                     /locus_tag="OO335_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR6004192.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="OO335_004027"
     CDS             complement(1683..3110)
                     /locus_tag="OO335_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR6004193.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="OO335_004028"
     CDS             complement(3121..3840)
                     /locus_tag="OO335_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR6004194.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1086 c          869 g         1124 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcaccccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DAJTSF010000200         4183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000200 DAJTSF010000000
VERSION     DAJTSF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.206"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(348..995)
                     /gene="ychE"
                     /locus_tag="OO335_004029"
     CDS             complement(348..995)
                     /gene="ychE"
                     /locus_tag="OO335_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6004195.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1472..4147
                     /gene="adhE"
                     /locus_tag="OO335_004030"
                     /gene_synonym="adhC"
     CDS             1472..4147
                     /gene="adhE"
                     /locus_tag="OO335_004030"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6004196.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1168 a         1021 c          960 g         1034 t
ORIGIN      
        1 aattcaatca aatagagatt ggctaagcat gtctatcttt tctgattaca gttccagtag
       61 tgaaatgcac aataatttaa caattgatta ttatttagca ctttcctcta ccaaaggcag
      121 tggaattact aatatcattt caattattct tcagcaagcc caggattatg atgtggcgaa
      181 aataacatga tggtgaggag aagcagttaa ggaaatcctt ataactaact gtaatttgag
      241 aagaatattt ttacctattc cttttgcttt tgataactgc gcgggaacaa agtaaaaata
      301 atgacgaagt aaacggagca tggcagctcc gtttcatttg aaaggaatta attaagcagg
      361 ccggggaaaa tccccttaat accagtaacg ataaattcaa tccccaatgc catcagcaat
      421 agccccataa tacgcgtaat cacgttgatg ccggtctggc gtaaaacccg taccagccac
      481 ggtgccatgc ggaacaatcc ccagcaacat aaagcgaaca atgcaatagc cacaaagaaa
      541 ccaaacagat agctaatgct gtgataacgc gtaccccaga cgatggtaga actgatcgcc
      601 cctggccccg ccatcaacgg caacgccaat ggcaccacac caatactttc acgtaccgcg
      661 gtttctgatt tttcttgctt gttctgttta tcctcgccaa gcttgccgct gatcatcgac
      721 atcgctatcg tcaccaccag gatgcccccg gcgatacgga acgaatcaat tgatatacca
      781 aaaagttgta gaatcgtgtc gccgagaaaa agcgagatcc acaagataat ggccacagac
      841 aggttggctg taaggttagt tttgtttcgc gctgccgctg tctgataact ggtcatgctg
      901 ataaagacgg gaataatccc taccgggttg accagcgcaa ataacccgat gaaaaatttg
      961 aagtaaacgg gaaaatcaaa aagggtctga atcacggttc gctccgaagc aaaagccgga
     1021 taatgttagc cataaataag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
     1081 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
     1141 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
     1201 aagcgatgct gaaaggtgtc agctttgcaa aaatttgatt tggatcacgt aatcagtacc
     1261 cagaagtgag taatcttgct tacgccacct ggaagtgacg cattagagat aataactcta
     1321 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
     1381 atcggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
     1441 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
     1501 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
     1561 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
     1621 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
     1681 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
     1741 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
     1801 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gcctgaagac
     1861 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgcaacca acaaagcggc
     1921 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
     1981 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
     2041 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
     2101 cggtgtaggc gcgggtaaca ctccagttgt tattgatgaa actgctgata tcaaacgtgc
     2161 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
     2221 gtctgttgtt gttgttgact ctgtttatga cgcagtacgt gaacgtttcg caacccacgg
     2281 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaatgg
     2341 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
     2401 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
     2461 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
     2521 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
     2581 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
     2641 aacagctcgt atcctgatta acaccccggc ttctcagggt ggtatcggtg acctgtataa
     2701 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tggggtggta actccatctc
     2761 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
     2821 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
     2881 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
     2941 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
     3001 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
     3061 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
     3121 cgccgcgaag atcatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
     3181 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
     3241 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
     3301 tgtaactgac gacgctactg gtcagaaata cccgctggcg gactatgcgc tgaccccgga
     3361 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
     3421 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
     3481 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
     3541 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
     3601 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
     3661 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
     3721 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
     3781 ggctcgccgt cgttatgctg aaattgccga ccacctgggt ctgagcgcac cgggcgaccg
     3841 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
     3901 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
     3961 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
     4021 gatctccgag ctgaaacaga ttctgctgga tacctactac ggtcgtgatt atgtagaaga
     4081 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
     4141 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttc
//
LOCUS       DAJTSF010000201         4182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000201 DAJTSF010000000
VERSION     DAJTSF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.207"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..186)
                     /locus_tag="OO335_004031"
     CDS             complement(<1..186)
                     /locus_tag="OO335_004031"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR6004197.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="OO335_004032"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="OO335_004032"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6004198.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="OO335_004033"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="OO335_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6004199.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="OO335_004034"
     CDS             3863..4144
                     /locus_tag="OO335_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR6004200.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1043 c         1062 g         1061 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagt
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAJTSF010000202         4177 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000202 DAJTSF010000000
VERSION     DAJTSF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.208"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="OO335_004035"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="OO335_004035"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6004201.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="OO335_004036"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="OO335_004036"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6004202.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3324)
                     /locus_tag="OO335_004037"
                     /pseudo
     CDS             complement(2698..3324)
                     /locus_tag="OO335_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3454..>4177
                     /locus_tag="OO335_004038"
     CDS             3454..>4177
                     /locus_tag="OO335_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6004203.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEG"
BASE COUNT         1087 a         1103 c          962 g         1025 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccaag
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatcac attaccaggc
     3061 ggtataacaa cgccagagtg ataaagacga taagtaccgg caccatcggc acatcatcat
     3121 aaaacgccac atctcccgcc gctgaaccca gcgtgagaat gattaacact tcaaacagcg
     3181 acatctgtcg cacaccgcgt cttccggtca ttttgaggaa caaaaaacca gtacaaaggt
     3241 ataaagacta cgtagtgcaa cttcgccaag gaaatcaaaa ggcactttat caaatgccat
     3301 acggtggaga tcgaacgcct tcatttttat gctcttaacg tggtttatcc gctaaaagca
     3361 tagtcaacag ttcatcggca tggcggcggt gatagcgcct ttgagataaa acccgtatta
     3421 ttgcgcgctt tccgtacgac taaagtgatt ttcatgacgc aaacatttat ccccggcaaa
     3481 gatgccgctc tggaagattc catcgctcgc ttccagcaaa aactttcaga cctcggcttt
     3541 cagattgaag aggcctcctg gctgaatccc gtgcctaacg tctggtctgt acatattcgc
     3601 gacaaagagt gcgcactgtg ttttaccaac ggtaaaggcg caaccaagaa agcggcgctg
     3661 gcttctgcac tcggtgaata tttcgagcgt ctctcaacca actacttttt tgctgatttc
     3721 tggctgggcg aaaccatcgc caacggtcca tttgtgcatt atcccaacga aaaatggttc
     3781 ccactgagcg aaaatgacga tgtgccagaa gggctgctcg atgaccgtct gcgcgcattt
     3841 tacgatccgg agaatgaatt gaccggcagt atgctgattg acctacaatc cggtaacgaa
     3901 gatcgtggta tttgcggtct gccgtttacg cgccagtccg acaatcagac cgtttatatt
     3961 ccgatgaata tcattggtaa cctgtacgtt tctaacggta tgtccgctgg caatacccgc
     4021 aacgaagcac gcgttcaggg gttgtccgaa gttttcgaac gctacgtgaa aaaccgcatt
     4081 attgctgaaa gcatcagctt gccggaaatc ccggcagacg tgctggcgcg ttacccagcg
     4141 gtggttgaag cgatcgaaac actggaaaca gaaggtt
//
LOCUS       DAJTSF010000203         4166 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000203 DAJTSF010000000
VERSION     DAJTSF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.209"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..332)
                     /gene="flhe"
                     /locus_tag="OO335_004039"
     CDS             complement(<1..332)
                     /gene="flhe"
                     /locus_tag="OO335_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR6004204.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(332..2409)
                     /gene="flhA"
                     /locus_tag="OO335_004040"
                     /pseudo
     CDS             complement(332..2409)
                     /gene="flhA"
                     /locus_tag="OO335_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2402..3549)
                     /gene="flhB"
                     /locus_tag="OO335_004041"
                     /pseudo
     CDS             complement(2402..3549)
                     /gene="flhB"
                     /locus_tag="OO335_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3785..>4166
                     /locus_tag="OO335_004042"
     CDS             3785..>4166
                     /locus_tag="OO335_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR6004205.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1037 a         1207 c         1021 g          901 t
ORIGIN      
        1 ccgcctggca cttcccagat aaatcgcaac ggttctgctg ccgctatacc ggaaaaggcc
       61 acggtggtac cgctctgccc ctctaattcg acacaacgag attgcgaaca caagcgaacc
      121 cgcagtcctg caggtgtcgg gccgataagc tgataacgcc acgccaccag cgtcatcaat
      181 cctgaagcag gttgtcgcgc agaaagaggt gcagacgaca tcgactcacc gcgatgattt
      241 agcgtaattc cgacactact tgcctgccac atcccctccc cggcggcttg caccagcagc
      301 ggaaacaata atatcgctaa taaggttctc atcatttacc gccaattgtc gccgtcatgc
      361 ggatatgtcg gttatcagac agttccagat tcgacaggac cactaactgc ggcaagctgc
      421 ggcgcaggaa gcgagacaat aatggtcgca gcgcgtggtt caccaacaat actggcggcg
      481 cacccagcat ctcctgacgg gatagagctt cctgagtttg cgccagtaaa cgatccgcca
      541 gccctggctc cagtcctccc ccgccctgca gcgcctgtag taacaaacgt tccagcggtg
      601 tatcgaggcc aataacatgg acttcatctt tgccaggaaa ccactgctgg gtaatcgccc
      661 gtcccaacgc cacgcgcacg acggcgctta attcatgtgg atcgctttgg atgggcgcat
      721 gttccgccag cgtttcgaga atggtgcgca tatcgcgaat cggtactttt tcatcgagga
      781 gattttgcag cactttatgc agtgtggtga gcgtgacgac gccaggaacg agatcttccg
      841 tcagctttgg catctcctgg gcgacgcgat ccaacagctg ttgcgcctcc tgacgaccaa
      901 acagctctgc ggcatgctgg ctaatgaggt ggttaagatg cgttgctacc accgtgctgg
      961 cctcaaccac tgtgtacccc tgaatctgcg cctgttcttt tagcgcactt tcaatccaga
     1021 tagcattcag gccaaatgcc ggatcgacgg tcgcctcacc aggtaacgtc ccggcagcgg
     1081 ttccagggtt aatcgccagc cagcgccccg gataagcatc accactgcca atctccacgc
     1141 ctttcatcaa aatgcgatag cgggcaggtt gcagatccat attgtcgcga atgtgcacca
     1201 ctggcggcag aaatcccatc tcctgggcaa atttcttgcg gatactgcgt atacggccca
     1261 acaactcacc atcctgctgg aaatcgacca tcgggatcag tcgataaccc acttccattc
     1321 ccagagaatc ttccagttgt acatcgttcc acgtcgcttc gacaacggta ttattctctg
     1381 ccatttttac cggtttgggt tcggcaggcg ctttttgttc gcgtccgcgt atccaccagg
     1441 ccagccccag caatccggca gtgaacagca aaaataccag gttcggcatt ccaggcacca
     1501 ggccgagtaa accgagcacg gcggcgctta acaacataac gcttgggtta ctgaaaagct
     1561 gattcaccat ctgctcgcca acatcctgat cggtgctgac acgcgtaacg atgccccccg
     1621 cggcggtaga aatcaccagc gccggaattt gtgccaccag accgtcgcca atggtcaata
     1681 gcgtataact ttccgccgcg tgtcccatgc tcatgccagt tgcagcacgc cgaccagcaa
     1741 cccgccgaca acgttaatga ccatgatgag gatcccggca atggcatcgc cgcgaacaaa
     1801 cttacttgcc ccgtccattg agccgtaaaa atcggcttcc tgagtcactt cggagcggcg
     1861 ttttttcgcc tcatcttcac caatcaatcc agcgttaagg tcggcgtcaa tcgccatctg
     1921 cttacccggc ataccatcga gaacaaagcg cgcacccact tctgctatac gcccggcacc
     1981 tttggtaatg accataaagt tgatgatcac gagaatgaca aacaccacga taccgatagc
     2041 gaaattgcca ccaacgagga agtgaccgaa cgcttcgacc accttcccag ccgccgccgc
     2101 gccggtatgc ccttccatca aaatgatacg ggttgaagcc acgttaagtg ccagacgcaa
     2161 caacatagta aacaacagaa tggtcggaaa cgcagcaaac tcaagcgtgc gctgggtaaa
     2221 catcgccacc agcaacacca tgatcgacaa ggcaatattg aaggtaaaca acaggtcgag
     2281 tatgaatgcg ggcagtggca gcaccatcat cgacaagatc aacaggatca aaatcggtcc
     2341 ggcaaggatc tgccattatg tcgatttcag gtttgcgggc aggcgcagca tcgcggccag
     2401 attactcatg ggtcggtttc tcgttaataa aatccagggc tgccggcacc ggaagatgag
     2461 taggtgtaca gggcgctgtc caccagccag acgccagcgt ttcagttgcc agacccaggc
     2521 cagcacttcc gccaccgcgg cgtacagttg acccgggatt tgttgaccaa tctccgcatg
     2581 tcgatacagc gctcgcgcca gcggcggcgc ttcaagcgtc gggacgttat tttcagcgcc
     2641 aatttcacga atgcgcagcg cgaccagccc tgcaccttta gcgaccactt tcggtgcgct
     2701 cattttgttt tcgtcatact gcaacgctac cgaatagtgg gtcgggttat tgacaatgac
     2761 atccgctttc ggcacatcgg ccatcatccg acgccgtgcg gcagctcgct gcatctgaca
     2821 gatccgtcct ttaacatggg ggtcgccttc gctttgtttg aactcatcac gaatatcctg
     2881 ccgcgacatt cgcagctttt tcaggtggct gaagatttgg aaaaagacgt caaatcccac
     2941 cattggaatg acaccaagca ccaccagcag tgcgcatagc cctaccaaat ccatcgcatt
     3001 acccatggcg gtaatcggtg actcggccat taagcgcatc atctgcggcc agtgatgcca
     3061 gagaaaaaac cccgtcacgc tgccaaccag gatggttttc aaaattgcct taagcaactc
     3121 cgcgctagtc tgtgccgaga atatccgttt aatgcccggt agcgggttga gtttggaaaa
     3181 cttcggctgc aaggatttgc cgctaaatac cagccccccc agcataaccg gagaaatgag
     3241 cgccaccagc accacgccgc taatcagcgg tagcagcgcc agcatggctt ctctgatcag
     3301 cagaataatt tgcccgagga tcagattcgg gtctttgata atactgtgat caaaatgcag
     3361 cccagcggag agcatgcccg acaatcgacg ggccagcgac acaccgccaa accagataac
     3421 actaacgccc accagcaaaa tcagcagtga ggtcagttca cgggaacgcg ggatttgccc
     3481 ctcttcccgc gctttttcta gtcggtgagg tgtgggggct tctgttttgt cgtcgctctc
     3541 gtcagacacg tcgccaatcc tggaaagagt attaagcgta aatgatgcca gagcgcaaag
     3601 cgttcaatgg tttaagtaag gggcaaaaca ggcgggattt agggcgttta tgcaaaaata
     3661 ggttcagttg cagaacgttt tgcgaaccat tttccagttc tgatatttag ccatttagcc
     3721 tatagaatcg cactccgcct ctctgttagt ttgctaataa ttaaattatt acaaaggtga
     3781 tcaaatggaa tggcaacaat tgcttaactg caaccgtcgg aaagataatc taaaaaaaac
     3841 caacatagca tttttacatc cccttgctag cggaagacaa gagatcgagc gggattacga
     3901 ccgattactt tttgctgcac caactcgtcg gcttgcggat aaaacacaag ttttcccgtt
     3961 agatcaaaat gacagcatac ggacgaggct gactcattct cacgaagtag caaactttgc
     4021 tcgcgggata ggaatgaggc tcgctttcga gatgaaggag cacatctttg gcgaaatacc
     4081 cacggggatt gtcgttgaac gcgacgttcc tgctctgtta gcagcgatcg gactggcaca
     4141 cgatctcggt aatccaccat ttgggc
//
LOCUS       DAJTSF010000204         4115 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000204 DAJTSF010000000
VERSION     DAJTSF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.210"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..158)
                     /locus_tag="OO335_004043"
     CDS             complement(<1..158)
                     /locus_tag="OO335_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6004206.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="OO335_004044"
     CDS             complement(308..469)
                     /locus_tag="OO335_004044"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004207.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..654)
                     /locus_tag="OO335_004045"
     CDS             complement(466..654)
                     /locus_tag="OO335_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6004208.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="OO335_004046"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="OO335_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6004209.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="OO335_004047"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="OO335_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="OO335_004048"
     tRNA            complement(2161..2237)
                     /locus_tag="OO335_004048"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2340..2415)
                     /locus_tag="OO335_004049"
     tRNA            complement(2340..2415)
                     /locus_tag="OO335_004049"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2380..2382),aa:Ile,seq:cat)
     gene            complement(2560..3381)
                     /locus_tag="OO335_004050"
     CDS             complement(2560..3381)
                     /locus_tag="OO335_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6004210.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3396..3752)
                     /locus_tag="OO335_004051"
     CDS             complement(3396..3752)
                     /locus_tag="OO335_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6004211.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3765..>4115)
                     /locus_tag="OO335_004052"
     CDS             complement(3765..>4115)
                     /locus_tag="OO335_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6004212.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1155 a         1119 c          793 g         1048 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gcccccccgc
     2281 accaacaacc ctgttatcgt gtcgtctgct cttcctgata agctaatggc ggtttgtgat
     2341 ggtggccctt gctggatttg aaccagcgac ctggcgatta tgagtcgctc gctctcacca
     2401 ctgagctaaa gggccgggag cagaataata acggtccgta attaatttcg caataaaaaa
     2461 acccgctcag cggcgggttg tagaaactct tctaacgcca ggcataaaac gcccatcatt
     2521 atgacgaatt taccacagat tccggaaaaa tcaaccttgt tacctagtta ccttttttaa
     2581 ctgccgctca gcccatgctt cttcaatatc aaaccgggtc accagcgcat cataaaattt
     2641 cttaactgtt ttttcccatg acgcgcgtgt tatctggttt gtcacctcgc atatagcatt
     2701 aaatgcctcc gttgatggta gtctttcata gccacgacca ccacaacgct ggcagtctct
     2761 gataacaggc ataccacgtt ttaccgactc ttcacgatga atggcgacac cacgcccacg
     2821 gcaatcctta caggcggtgg aaacctcacc ctttccgcca cactccggac aggcaacttt
     2881 taccacctcc ctgacttttt tccattcttc ccagtaaaac ggatacacac ctttcgtaca
     2941 ctttgcccat accggcggct taccatccgg atactggatc ttgtttgtaa aaactatgct
     3001 ttcaataaat ttttccccat agcaacaagg gcactgcttt ttactcgctg cgctgcgggc
     3061 ataatcctca aaagcgtacg aagccataat gcgcatcact accggtttta tttctgccgg
     3121 aagttttctc aacgccgcca cacgatcgca ccgactgagt gcataatctg ccagtaattc
     3181 tgttgcccgc gccctgtcat tcatactgat gcccattttc cccaggaacg cagaaaaacc
     3241 catctcagcc cgattctgtg tcatgccctg cgcggccatc acatcagtga tactcagcgc
     3301 atcttttgac gttgaggccg atgcatcggt caggccaggg gattttgggg agtagtattt
     3361 cggtaaatct tccagtttca ttttttgacc tgctcttaat gcattatggg gtaaatcttc
     3421 acccccagac gtccaccaga tactggctga ccacgaacga tattgatttc atcaaactgc
     3481 tcatcgtcca ttaacactcc cgcatgcgtc agcgcatcca gcggtgcttt caggatattg
     3541 tccaggtcgc gacgacgctt atccggtggc tctgcaatca cctttatcgc cagccttccg
     3601 gacaggctta atttcagccg ctgctggcga acaataagcg ccacagcccg gcgataacgc
     3661 tttccctcct ccgagataaa atatgtgctg ccacggcgtc gccagtaagt gttcaccgtc
     3721 ggcgggtaag gtaaaaccaa atctatgagc atcagtcacc tcttttaccc aagcacgcca
     3781 gttgcaaagg cgtgatcaag aaaacgaaaa attaaatcaa cctgagaacc atgcttttct
     3841 tcgaacgcca gaggatccgc atgaagctcg ttgtgatgct cccgacacag cggtagcgtg
     3901 aaaatatcgt gagatttggt ccccattccg ccctgaccat gaccaatcag gtgatgggga
     3961 tcgtcggctg gcttaccaca acacgcacac ggctgtgtct tcacccagcg tttgtatttc
     4021 tcgttaaccc agcggcgacg tttaggtcgt ttcatgaaag attccggaga ctcaggatca
     4081 acggcaatgc tgaccaccgt cttttcctgt tgtgg
//
LOCUS       DAJTSF010000205         4092 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000205 DAJTSF010000000
VERSION     DAJTSF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.211"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..247)
                     /locus_tag="OO335_004053"
     CDS             complement(<1..247)
                     /locus_tag="OO335_004053"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Converts N-acetylmannosamine-6-phosphate to
                     N-acetylglucosamine-6-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6004213.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPV"
     gene            complement(295..1785)
                     /gene="nanT"
                     /locus_tag="OO335_004054"
     CDS             complement(295..1785)
                     /gene="nanT"
                     /locus_tag="OO335_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6004214.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(1895..2788)
                     /gene="nanA"
                     /locus_tag="OO335_004055"
     CDS             complement(1895..2788)
                     /gene="nanA"
                     /locus_tag="OO335_004055"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6004215.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(2910..3701)
                     /gene="nanR"
                     /locus_tag="OO335_004056"
     CDS             complement(2910..3701)
                     /gene="nanR"
                     /locus_tag="OO335_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6004216.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1068 a         1078 c         1018 g          928 t
ORIGIN      
        1 gtaccggaga atcctccaga tcgcgtttca caattccaat aatcggcacg ctcaccaccg
       61 cacgcgtggc ttgcagattt gccacacctt cgatgcgaat ggcaaccgcg cccgcctgct
      121 ctgccgctaa tgccatggct gcgacgattt cgggtttatc gagcgggctg tcaggaaccg
      181 gctggcagga gacaatcagg ccaccgttag cagcgatttt ttgatccagt tgtgcaagta
      241 acgacataca tcttccctta gcgaaaggcc cggtacatag accgggcaac aggattaact
      301 tttggttttg actaaatcgt ttttggcgct gccaaatggc acggcaccgc taaatggttt
      361 gccgtcgata gcgtcatgag tacgcaatgc ttccgggcgc agccaacgct gaacgcggga
      421 aggcatatcc agaccaatca gcaggatcac cacaaatgtc aggctgaacg agagcgatgc
      481 cagcgcagta cccagatcca gacgttgagc gatcaacgcg ccgatgattg gggccagtgc
      541 gccacccaat gcgccaacgt tgtaggtaaa gcccaggccc gctgcacgct ggtcggtatc
      601 gaaataaccg ccaatcagtt tcggtaagat cccggcgatc ccttgtccaa gcatttgctg
      661 gaagaacagt aacagaccga gcacccagac gtttgcgccg ccaatcgcaa ataccggaat
      721 aatcagcagc tgcgaggcca gcaggctaca aacgtacgct ttgcgggttc ccagccagtc
      781 accgaggaag ccacctacgc agcatcccac ggccgcgcca aagccactaa agaacagcac
      841 attggcgaca gtatgcgggt tataagccag atcggttttc agatacgttg gcagcagcgc
      901 ctgaatcggc catgagtaga ggaaagcaaa caacacgacc accatcagca ttacgcccgt
      961 tggccagcgt ttacccgtac tttgcaccat aaagctgata aagattgcgg cgcataacag
     1021 cccgagaaca gcgacgattg cagcattttg caggttaccg gcgaagcaga accacagcgc
     1081 agtagccgcc gccagtgtca ttacgatatt ggcaatgcga tgttcaccac ggtagagaat
     1141 atccaccatt gtgcgtactg gtgctttacc tgcgtgtttc tctttccagt cttccgcttc
     1201 cgggatgttt ttacgcagcc agagagcaaa gatgattggc aaaatgccga taaagaacag
     1261 cgcacgccaa ccccagaccg gaaccaccag gctatagacc tgagcggcaa cgacggcccc
     1321 cacagagaag cctgaaatca aaaaaccact ggctttgtta cgcagatgtt ttggccagct
     1381 ttcaatgaca taggtggcgc tggaaccgta ttcacccgcc atccccatgc cgatgaccag
     1441 acgagcgata aacatggtga tgtagcctgg cgcaaagccg caggccagcg tcccggccga
     1501 gaagagaacg atgctggtga ccattgccag acgacgcccg tagcggtcac ccatagcgcc
     1561 gagcatcagg ccgccgaacc agcgagagat aaaggctgcg gagatcagac ttgccgcctg
     1621 caccgtcgtc agcccgaatt caccctgtac ttcggtgagt accaaggcga ttaaaacgaa
     1681 atcaaaaccg tcaagcagat atcccaacca ggcagcggaa aatgcgcgcc attgtgcacg
     1741 gttgagatgg cgataccacg ggatattctg ggttgtagta ctcattgtga gtctcccgcg
     1801 gtgggcgatg cccacacgct ttggtatgaa aattgtaggg tacagatgcg tttatttccc
     1861 ctcacccggt aggggcgagc gaggggaaaa caactcaccc gcgctcttgc atcaactgct
     1921 gggccagcgc cttcagttct ggcagatatt tttcatctac cggtccaaac ggtttgcggc
     1981 acagcggcac agaaacgaca tccatataat gcaggacagt tttcaggccg cggaatacgc
     2041 ccgttttgat cagtaaatca atgactttgt tgcactcagt ttgcagtttc tgcgcggtct
     2101 ggatatcgcc ttctttcagc gccttcacga tcccctgata tcgccagccc atgatgttgt
     2161 aagtactgcc gataccacca tcagcgcctg ccagcaagcc ggaagcgaag atttcgtcgt
     2221 aaccgttata gagcaccaga tcaggatgtt cacgacggat ctgttccatc tgatagagat
     2281 cgccagaagt ctgtttcagc gcaccgacgc caggcaacgt aaccagggtg ttgatctgat
     2341 ccagggtcag ttttacccca ctcagggctg gaatgttgta caccaccatc ggcaaaccat
     2401 ccgctgaatc aataattgcc cgatagtgat cgcagtgttc ttcaaagctg aaaggatagt
     2461 agaacggcgt gacggcggag acggcatcga agccataacg tttagccgat gccgcaagtt
     2521 gttggctttc ggcggtgctg acgcaaccga cgtgggcgat gagtttaatc ttacctttcg
     2581 cctcttcggc gacgatttcc agtacctgtt cacgctcgga aaggctttgt acaaaggctt
     2641 cgccggtcga accacccacg tataaaccgt cgatgccctg ctgaatattg aactgaacca
     2701 ggcggcgcag actcgcttta tccagtgctt gttgttgatc aaaaggagtc aggagtgcag
     2761 ccattacgcc acgtaaattc gttgccataa atacctctga agtgatgctt gtctgataaa
     2821 cgatatacct ttatacctgt tataccagat caattaagca acaccccata cagaaagctt
     2881 ataatgcgat ctgcttcact aaagtggcat tatttctttt tgttggtggt ctgaccgaaa
     2941 gcgtgccagg tagcagagac gctgttgaga tgcgattgca acgcacgatc ggcttcgtca
     3001 ggatcatgac ggcggatcgc atcaacgatc gcaatatgct gttgataact aacgttgtta
     3061 tgttcgtgca gtgcctgatc ggtaaccgtt gggcgtgcgg caataagcca gtcgagcagg
     3121 gcaacgtgga tcgccatgaa gattgggtta ccggggatct ccgccagcac gcggtggaaa
     3181 tcaacgtctg aacgaatgaa tgccgcgttg ttatccagcg actgactgtt gatttccagt
     3241 gcttttgcca gcaaatcgat ttgctcatcg gtggcgtgtt cagccgcata gcgcaccagg
     3301 ctggattcaa agaacagacg taattgttcg aaatgggcaa tcccaccagg atgagaaagg
     3361 aaatctttcg ccatgccgga aagctcgccg atgatagtgt ccgcagaagg tcgagagaca
     3421 cgagcgcgtt cgccgttgtt tatttgcacc agacctttgc gttttaacgc tgccagcgct
     3481 tcacgcacag aaggacgccc gacgttaaag aacgccatca gttcgcgttc agacggtaat
     3541 tgttcacctt cgccaaattc acgacggcgg atcatctgtt ccagctcttc ttccaccatt
     3601 tcggagagtt ttttacgcgc cagcgggcgg ctacgcaagt tgcgaccaat tgcaggtgaa
     3661 gaatcttcgg tttgcgaatc aaatgcgttc ataaggccca ttctgtaagg tcagtgtgat
     3721 taacatcatc agtgacatcc tatcacagga ttgaaagtag gggaaaatgg caggtttttc
     3781 tctttgtgcc tcatcattac cataattaac ggaataatta actattgcga aaaattaatg
     3841 taacgcagat aaacacatcc cgattgaatt attcataaga ctattcacaa gcattatgaa
     3901 tattatgaat gtgttcttac aaaataatca taatcgcata ttttttaatg aaaaatcacc
     3961 tcacctacaa ttaaaaacac gacatccgca ccataaatag acttgcaaaa aatataacat
     4021 cattgttttc aatctgccat ttatgggatt gaccgttttc ttttgacacg gagttcaaca
     4081 atgggtaatg ct
//
LOCUS       DAJTSF010000206         4064 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000206 DAJTSF010000000
VERSION     DAJTSF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.212"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..662)
                     /locus_tag="OO335_004057"
     CDS             complement(<1..662)
                     /locus_tag="OO335_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HCR6004217.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QY"
     gene            complement(720..1244)
                     /gene="sat2"
                     /locus_tag="OO335_004058"
     CDS             complement(720..1244)
                     /gene="sat2"
                     /locus_tag="OO335_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6004218.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(1339..1812)
                     /locus_tag="OO335_004059"
     CDS             complement(1339..1812)
                     /locus_tag="OO335_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6004219.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2144..2680
                     /locus_tag="OO335_004060"
     CDS             2144..2680
                     /locus_tag="OO335_004060"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR6004220.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            2795..3121
                     /locus_tag="OO335_004061"
     CDS             2795..3121
                     /locus_tag="OO335_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6004221.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(3309..3548)
                     /locus_tag="OO335_004062"
     CDS             complement(3309..3548)
                     /locus_tag="OO335_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6004222.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         1092 a          941 c          886 g         1145 t
ORIGIN      
        1 tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt
       61 ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg
      121 ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga
      181 tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta
      241 tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag
      301 atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga
      361 taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc
      421 tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt
      481 gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg
      541 ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc
      601 tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc
      661 atgatgttta actcctgaat taagccgcgc cgcgaagcgg tgtcggcttg aatgaattgt
      721 taggcgtcat cctgtgctcc cgagaaccag taccagtaca tcgctgtttc gttcgagact
      781 tgaggtctag ttttatacgt gaacaggtca atgccgccga gagtaaagcc acattttgcg
      841 tacaaattgc aggcaggtac attgttcgtt tgtgtctcta atcgtatgcc aaggagctgt
      901 ctgcttagtg cccacttttt cgcaaattcg atgagactgt gcgcgactcc tttgcctcgg
      961 tgcgtgtgcg acacaacaat gtgttcgata gaggctagat cgttccatgt tgagttgagt
     1021 tcaatcttcc cgacaagctc ttggtcgatg aatgcgccat agcaagcaga gtcttcatca
     1081 gagtcatcat ccgagatgta atccttccgg taggggctca cacttctggt agatagttca
     1141 aagccttggt cggataggtg cacatcgaac acttcacgaa caatgaaatg gttctcagca
     1201 tccaatgttt ccgccacctg ctcagggatc accgaaatct tcatatgacg cctaacgcct
     1261 ggcacagcgg atcgcaaacc tggcgcggct tttggtacaa aaggcgtgac aggtttgcga
     1321 atccgttgct gccacttgtt aacccttttg ccagatttgg taactataat ttatgttaga
     1381 ggcgaagtct tgggtaaaaa ctggcctaaa attgctgggg atttcaggaa agtaaacatc
     1441 accttccggc tcgatgtcta ttgtagatat atgtagtgta tctacttgat cgatcaggct
     1501 tttgtatatc tccccaccac ctgaaacaat gacatgatcc gttattttct ttaggttggt
     1561 taaagcatct ttaattgatg gaaagatcaa tacgttctca ttgtcagatg taaaacttga
     1621 acgtgttacg accgcatact ttcggttggg taatgctccc attgattcaa aagtcttgcg
     1681 tccaaccaac agccattggt tataggtaat agctttaaac aggagctgtt cacctttggc
     1741 actccatgga atatcagggc cattcccgat aactccattc ttcgatatag ctaccattag
     1801 tgatagtttc acaattcttc ctcagaggtt aacattttat tagtaagcat gctcgttttg
     1861 tcacccgctg atgcttaccg ttaattaata taatcacttg tattaaatag actttcttta
     1921 aaaatacaag acactctgtt attacaaatc gtgcatgccg tctatcctgc ttgcacgatg
     1981 cacattatct cacccaaaga actcataaca ttttaattta tttagcatta aattaaaact
     2041 ctaccggtaa acaagcatct ctaggcgtaa aaaacaggca cttttttaaa tcccagctag
     2101 aataggctgt ataggcagac agttgcaaga caaggagcgt tttatgtcta acagtccatt
     2161 tttaaattct atacgcacgg atatgcgaca aaaaggttat gcgctgaaaa ctgaaaaaac
     2221 ttacctgcac tggattaagc gttttattct gtttcacaaa aaacgtcatc ctcagaccat
     2281 gggcagtgaa gaggtcaggc tgtttttatc cagcttagca aacagcagac atgtagccat
     2341 aaacacgcag aaaatcgctt taaatgccct agcttttttg tacaacaggt ttttacaaca
     2401 gccgttgggc gatattgatt atatccctgc aagcaagcct agacggctac cctctgttat
     2461 ctctgcaaat gaagtgcaac gcattttgca ggttatggat actcgcaacc aagttatttt
     2521 tacgctgctg tatggtgcag gtttgcgcat taatgaatgc ttgcgtttgc gggttaaaga
     2581 ttttgatttt gataatggct gcatcactgt gcatgacggt aagggtggga aaagcagaaa
     2641 cagcctactg cccacgcgcc taatcccagc aataaaataa ctcattgagc aagcgcggct
     2701 tattcagcaa gacgacaact tacaaggcgt agggccatcg ctgccttttg ctttagatca
     2761 caaataccct tctgcttatc gacaagcggc gtggatgttt gtctttccct ccagcacgct
     2821 ctgcaaccac ccgtataacg gcaaattatg ccgccatcat ctgcatgact ccgttgcgcg
     2881 aaaggcattg aaggcagccg tacaaaaagc aggcatcgtt agcaagcgtg tcacttgtca
     2941 tacatttcgt cactcgtttg ctacgcatct attacaagcg gggcgtgata ttcgcactgt
     3001 gcaagaactc ttagggcata acgatgttaa gaccacgcaa atctatacgc atgtgttggg
     3061 tcagcatttt gccggcacca ccagtcctgc ggatggactg atgctactta tcaatcagta
     3121 aaagtagata aatatcttta tgtagccctg agtagcccct gatacttttt atggtgaaaa
     3181 agataaacaa tcatagaggg gataaatatg agccagttct accattgttc agataagttt
     3241 aagcaagata ctgtcaatca cagtgaccat tcatggtcat tacacttcct ccagttgcca
     3301 gacaatgcct aacataaaaa cgcaaaagcc caaaagtcag agcttaggtg ttctaggcgt
     3361 tttctaagcg ctgaagaaat acgtcttacc gtagattgcg tgcgtttctg aagcacattt
     3421 tccttataca tggctttatc ccaggcagct tcatcaagct cttggagtaa caagccagta
     3481 ataatgcgac actctcgggt ttgtagcgag ccgctggtga gatcggagtt atagcggaaa
     3541 ggggtcattt gtaattattt gtgggtggag gatagttaac ttattattgt agttggaaga
     3601 ggctgctttc caaacattag cacctcgtaa tgcttgttac tgtatattaa tttatgctct
     3661 tagcgcatgt taatgggcta ttacaatgca taatgtccac atcaagataa ctgtggctta
     3721 acacctgtac aagcgaaaaa aatcaatcac ctgtccggaa atacgggatc actgtaaagt
     3781 gttggtgagg cagtctagga gggggattta atttgggata ctgatgaaag gattatgatc
     3841 agttggatat aggatcgtac taagctgagc ggcaggttag ctagattagt taaccagata
     3901 agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct
     3961 acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc
     4021 cacccctccc agttcccaac tattttgtcc gcccacagct gccg
//
LOCUS       DAJTSF010000207         4042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000207 DAJTSF010000000
VERSION     DAJTSF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.213"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(886..1077)
                     /locus_tag="OO335_004063"
     CDS             complement(886..1077)
                     /locus_tag="OO335_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6004223.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="OO335_004064"
     CDS             1142..1465
                     /locus_tag="OO335_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR6004224.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1407..2165
                     /locus_tag="OO335_004065"
                     /pseudo
     CDS             1407..2165
                     /locus_tag="OO335_004065"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="OO335_004066"
     CDS             2143..2673
                     /locus_tag="OO335_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6004225.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="OO335_004067"
     CDS             2680..2913
                     /locus_tag="OO335_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6004226.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="OO335_004068"
     CDS             complement(3459..3851)
                     /locus_tag="OO335_004068"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004227.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          917 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAJTSF010000208         3963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000208 DAJTSF010000000
VERSION     DAJTSF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.214"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..221
                     /locus_tag="OO335_004069"
     CDS             <1..221
                     /locus_tag="OO335_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6004228.1"
                     /translation="QRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLPV
                     LQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            267..476
                     /gene="hha"
                     /locus_tag="OO335_004070"
     CDS             267..476
                     /gene="hha"
                     /locus_tag="OO335_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6004229.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            515..1105
                     /locus_tag="OO335_004071"
     CDS             515..1105
                     /locus_tag="OO335_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6004230.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1345..1605
                     /gene="repA"
                     /locus_tag="OO335_004072"
     CDS             1345..1605
                     /gene="repA"
                     /locus_tag="OO335_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR6004231.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1829..1903
                     /gene="tap"
                     /locus_tag="OO335_004073"
     CDS             1829..1903
                     /gene="tap"
                     /locus_tag="OO335_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR6004232.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1896..2753
                     /gene="repA"
                     /locus_tag="OO335_004074"
     CDS             1896..2753
                     /gene="repA"
                     /locus_tag="OO335_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR6004233.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3454..3645
                     /locus_tag="OO335_004075"
     CDS             3454..3645
                     /locus_tag="OO335_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004234.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT         1072 a          984 c          987 g          920 t
ORIGIN      
        1 ttcagcgaga tcgttatggt cgtattctcg ggcaggttta tgcaccagac ggaatgaata
       61 ttaatcagtt tatggtccgg gccggggctg cctgggtata tgagcagtac aacacagatc
      121 ccgtgctacc tgtccttcag aacgaagcca gacaacaaaa acgtgggctc tggtcagatg
      181 ctgatccggt tcccccctgg atatggaggc accgtaaata aagcgatggc aatcctatca
      241 ttcatctgtt ctcagcggga ataaaaatgg caaaaacaaa acaggaatgg ctgtatcagt
      301 tacggcgatg ttcatcagtc aatacgctgg aaaaaattat tcataaaaac agagacagtt
      361 tatcaaacag tgaacgtgaa tcatttaatt cagcagcaga ccaccggcta gcggaattaa
      421 tcaccggaaa gttatatgac aggataccaa aagagatatg gaaatatgta aggtaataat
      481 tcagcaataa ttaattgcca ggagcagata aataatgaag ttgattattt ttattctcat
      541 tgttctgatt attgcagcgc tacttatccg gattatcctc agaagtgtaa atcagcattc
      601 tcctttgctg atgcaattgc atgcggcagg tatacggact ggtgatgcag agcgaatact
      661 gtccagcggt gaatactggc aacgtcagaa gacgctgctg acagaaaggg aagtcagttt
      721 tatgaaagga ctgttcagaa ttgtggatat gaagcggtgg tatctgtgtc cgcaggtacg
      781 ggtagcggat atcgtccagc tgaacgggaa tatccggcca cgatcacgcc agtggtggca
      841 gttattcagg atggtgtctc agtggcatgt tgatgtggtt atcgttgagc tgcgttcgtt
      901 cagtattgtt gcagcagtag agctggatga tgccagccat ttacgaccgg aacgcagacg
      961 ccgggatatt cttctggaag aggttctgag gcaggctggt attccgttgc tcagaagcca
     1021 cgatgccaga aaactgctgc agatgaccgg agaatggctg aatacaacag gggctgatca
     1081 gcagtcaccg gaacatcgta gctgacgcct tcgcgttgct cagttgtcca accccggaaa
     1141 cgggaaaaag caagttttcc tcgctcccgg cgtttccata actgaaaacc atactatttc
     1201 acagtttaaa tcacattaaa cgacagtaat ccccgttgat ttgtgcgcca acacagatct
     1261 tcgccacaat tctcaagtcg ctgatttcaa aaaactgtag tatcctctgc gaaacgatcc
     1321 ctgtttgagt attgaggagg cgagatgtcg cagacagaaa atgcagtgac ttcctcattg
     1381 agtcaaaagc ggtttgtgcg cagaggtaag cctatgactg actctgagaa acaaatggcc
     1441 gctgttgcaa gaaaacgtct tacacacaaa gagataaaag tttttgtcaa aaatcctctg
     1501 aaagatctca tggttgagta ctgcgagaga gaggggataa cacaggctca gttcgttgag
     1561 aaaatcatca aagatgaact gcagagactg gatatactaa agtaaagact ttactttgtg
     1621 gcgtagcatg ctagattact gatcgtttaa ggaattttat ggctggccac gccataaggt
     1681 ggcagggaac tggttctgat gtggatgtac aggagccaga aaagcaaaaa ccccgataat
     1741 cttctttaac tttggcgagt cagaaagatt accggggccc acttaaaccg tatagccaac
     1801 aatcaagcta tgcggggagt atagttatat gcccggaaaa gttcaagact tctttctgtg
     1861 ctcgctcctt ctgcgcattg taagtgcagg atggtgtgac tgatcttcaa caaacttatt
     1921 accgccaggt aaagaacccg aatccggtgt tcacaccccg tgaaggtgcc cggacgcttc
     1981 cgttctgcgg gaaactgatg gaaaaggcgg tgggcttcac ctcccgtttt gatttcgcca
     2041 ttcatgtggc acatgcccgt tcgcttggtc tgcgtcggcg catgccaccg gtactacgtc
     2101 gccgggccat tgatgcgctg ctgcaggggc tgtgttttca ctatgaccca ctggccaacc
     2161 gcgtccagtg ttccatcacc acgctggcca ttgagtgcgg actggcgacg gagtctgctg
     2221 ccggaaaact ctccatcacc cgggccaccc gggccctgac gttccttgca gagctgggac
     2281 tgattaccta ccagacggaa tatgacccgc ttatcgggtg ctacattccg accgatatca
     2341 cgttcacatc tgcactgttt gctgccctcg atgtatcaga ggaggcagtg gccgccgcgc
     2401 gccgcagtcg tgtggaatgg gaaaacagac agcgcaaaaa gcaggggctg gataccctgg
     2461 gtatggatga actgatggcg aaagcctggc gttttgttcg tgagcgtttc cgcagttacc
     2521 agacagagct taagtcccgt ggaatgaagc gtgcccgtgc gcgtcgtgat gcggacaggc
     2581 agcgtcagga tattgtcacc ctggtgaaac ggcaactgac gcgtgaaatc tcggaagggc
     2641 gcttcactgc cagtcgtgag gcggtaaaac gtgaagtgga gcgtcgtgtg aaggagcgca
     2701 tgattctgtc acgtaaccgt aattacagcc ggctggccac agcttccccc tgaaagtgac
     2761 ctcctcagaa taatccggca cgcgccggag gcattcgcac gcctgaagcc cgccagtgca
     2821 caaaaaatca gcgctacata caaaaaacaa cctcatcatc caccttcagg tgcatccggt
     2881 cccccctgtt tttgatacaa aacacgcctc acagacgggg aattttgctt atccacatta
     2941 aactgcaagg gacttcacca taaggttaca accgttcacg tcataaagcg ccagacgtca
     3001 accctacagg gtgcaatgta tcttttaaac acctgtttat atctccttta aactacttaa
     3061 ttacattcat ttaaaaagaa aacctgttca ctgcctgtcc tgtggacaga caaatatgca
     3121 cctcccaccg caagcggcgg gcccccaacc gaagccggtt tagttacaac atgcaaacat
     3181 aaacccctaa aaaacccaag tccaacgcaa aactgaaacc acaacgcccc cctcataact
     3241 gaaaagcgcc gccgcccccg cccaaagggc gggaacagag tcgcttttaa ttatgaatgt
     3301 tgtaactaca tcatcatcgc tgtcagtctt ctggctggac gtatcgaata cacgctcgta
     3361 agcggccctc acggcccgct aacgcggaga tacgccccga cttcgggtaa aaccctcgtc
     3421 gggaccactc cgaccgcgca cagaagctct atcatggctg aaaacgggta tggcttggca
     3481 gggctgggga tgggtaaggt gaaatctgtc aatcagtacc ggctgacgcc gggcttcggc
     3541 ggttttactc cggtatcaca tgtaaccaca gcgtgccgcc ttccatgccg ctggcgcggc
     3601 atcagaataa tacaagcagc atttaatgca tttgcaaaag tgtgagtaat attaagtggt
     3661 cgtttcaggg atatcgttaa gacttgagcg ataggaatta aaaccccaaa aagattaaaa
     3721 aaacaccaca aaacggatgt ttcttcaaca ccacttttgc tccatatgaa cggaaccgac
     3781 gattaaactg gatggctctg attgattcag ggtatgaatg gcggtttttt gctccgtttc
     3841 cctcaaaatg gacgcaactt cccctctgcg gctctcagcc gcaccaccgc atccgggcca
     3901 gcggctcatg catcaggacc tgctctgcca gacggtagcc ccgcttcagc cccgtaaaac
     3961 gca
//
LOCUS       DAJTSF010000209         3806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000209 DAJTSF010000000
VERSION     DAJTSF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.215"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            437..2089
                     /locus_tag="OO335_004076"
     CDS             437..2089
                     /locus_tag="OO335_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6004235.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="OO335_004077"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="OO335_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="OO335_004078"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="OO335_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3526)
                     /locus_tag="OO335_004079"
                     /pseudo
     CDS             complement(2596..3526)
                     /locus_tag="OO335_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1052 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gaacagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaacgaag cctttaatat cgacttccac cacccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgta
//
LOCUS       DAJTSF010000210         3738 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000210 DAJTSF010000000
VERSION     DAJTSF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.216"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            108..2105
                     /gene="betT"
                     /locus_tag="OO335_004080"
     CDS             108..2105
                     /gene="betT"
                     /locus_tag="OO335_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCR6004236.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2168..3445
                     /locus_tag="OO335_004081"
     CDS             2168..3445
                     /locus_tag="OO335_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR6004237.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          904 a          852 c          941 g         1041 t
ORIGIN      
        1 ttccaccatg cattacgatt aacgccgcat agccagttga actttgctac tttgtgagag
       61 gtagtacctt ctatccagtg cgaatttaat taatggaata aatgattatg agtgaaaatg
      121 atacaatccc aaagaagtct acaagtcaga ttaacaaagc ggtattcttt acatctgctt
      181 tgctaatttt ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt
      241 ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc cttgctgtgg
      301 ccctgatttt actgagtgtc acgttccttg gactctcacg ctacggtgat atcaagctgg
      361 gcccggacca tgcgcagcct gatttcagct accactcctg gtttgcgatg cttttttcgg
      421 cagggatggg gatcggcctg atgttctttg gcgttgccga acctgtaatg cattatcttt
      481 cgccacccgt tggcactcca gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct
      541 ttttccactg gggactgcac gcatgggcaa tttatgccat tgtggcgctg attctggcgt
      601 tcttcagtta ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat cccattattg
      661 gcgatcgcat atacggacct gtaggacatg cggttgatat tttcgctgtt ataggcacgg
      721 tctttggcgt tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc
      781 atcttttcgg ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg
      841 ggttagcgac gatttcagtg gtgtccggtc tggataaggg aatacgtatc ctgtctgaac
      901 tcaatctggg tctggctttg ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc
      961 ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa
     1021 cgttcaacct ttacgcgtat gagcccaagt cgagcaactg gctggggggc tggacattac
     1081 tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct
     1141 cccgcgggcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc gcgggtttta
     1201 cgctaatgtg gatgacggtg tttggtaaca gcgcgatcta tctcattatg aaccaggggg
     1261 ccacagacct cgccaatact gttcagcagg atgtggcgct ggccctgttt aatttcctgg
     1321 agcatttccc gttctcttct gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct
     1381 ttgtaacgtc tgctgattcg ggggcaatgg ttgtggatac tctggcatca ggtggagtgg
     1441 caaacacacc cgtctggcag cgaatattct gggcctcgct catgggcatt gttgcaattg
     1501 cgcttctcct tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc
     1561 ccttctcagt gatcttacta atatctatat acggactttt aaaagctttg cgccgggatt
     1621 tgaccaagcg tgaaagcctg agcatggcga caattgctcc tacggctgca cgtaacccaa
     1681 ttccttggca gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac
     1741 gttttatgga cgacgttatc cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc
     1801 aggggacgat aagccacatt agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt
     1861 tgggcaacga gctgaatttt atatatgaag tgaggcttcg cgggtatatc tcaccgacct
     1921 tcgcgctcgc cgcaatggat aatgatgagc agcagactga acaacatcga tattatcgcg
     1981 ctgaggttta tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg aaccaggaac
     2041 agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg cacctggttc
     2101 gttaatagca acatgccgtc ctgggggcgg caattattat cccggccgca atatgaggga
     2161 atgcagaatg atttcacgct ggaaatggat gctgaagcag acaattaaaa aactatggtt
     2221 cagggcaacg ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa ttctttttaa
     2281 atcaatgata cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg ataacattct
     2341 gaacatactg gcatcgagta tgctggcagt gaccacattt tcgctgagta tcatggtcac
     2401 agcctacggt tcagccacta ctaatgtgac tcccagagct acgcgtttag ttgttgaaga
     2461 cgtcaccaca caaaatgtac tggccacctt catcggttct tttctcttca gtctggtagg
     2521 gattattgcc ctcaatatgg gagcttatgg agaaaggggg agagtcattt tattcattgt
     2581 cacactggtt gtcattgcct taatcctcat cacattgctt cgctggatac agcatttgac
     2641 ctctctgggg agggttggtg agacaacggc acaagtagaa caggcggcca tcgaaacatt
     2701 tattgcgaga gcaagaaatc cctgtctcgg tggatatcca tggcttgaga acaatgaaca
     2761 gccgaaagga acggttgcag tttatccgaa gaagattggc tatgttgaat atattgatat
     2821 ggtgaaactt agcaagctgc tgaccaatga tccccgtcat gtatacctcg tggcgcagcc
     2881 aggcagtttc atacatccgt ccatgccagt tttgtacctg agtcagggcc aggagtcgtc
     2941 aatcaacacc gatttacttg agacgattat tgtctcggat gcacgttcat ttgctcagga
     3001 tcctcgattt tgtcttagcg tcatggccga aatagcctgc cgggcccttt cccctgcagt
     3061 gaacgatcct ggaaccgcca ttgatgtcat tggcagaggt gttcgtatac tttccactta
     3121 cgcgcagaat aaatctgatg aaatagaagt gaaatatcct tcagtacatg ttgcaccact
     3181 tcagaataac gatctactgg aagacttttt ctcacctgtc gcgcgcgatg gtgccagtat
     3241 gagggagatt cagataagag tccttaaagg actgtcgatg ctgagtaaag gttggcctga
     3301 gatatttgct gaagccgcac ataccctggc atttgaaaca ttagagcatg caactcgtgc
     3361 tgaccatata gattctgata gatatctaat aaaatcaact tattataatt tatttagtgg
     3421 cgaatattct aataaaaaaa catagctgca gagcaagcaa ttcggttgtt ggcaagtcaa
     3481 gggcaaagca ttcgcgtgag aatgggcgta tgctttgtgc caaaagcaaa cgttgctaat
     3541 tctgcactgt tcagcgatgg cgcagtagag acaggcactg ccactgtcca gtcggcaaaa
     3601 gcgaacactt tgccactgcg gggataaact gattgaagct ctcgcgcaaa atcacttatt
     3661 cttttgcgtt aataaaatgt aggagatggg tcattgatcc tacccacgta atatggacac
     3721 aggcctaagc gaggttct
//
LOCUS       DAJTSF010000211         3548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000211 DAJTSF010000000
VERSION     DAJTSF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.217"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="OO335_004082"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="OO335_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR6004238.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="OO335_004083"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="OO335_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6004239.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="OO335_004084"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="OO335_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6004240.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAJTSF010000212         3511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000212 DAJTSF010000000
VERSION     DAJTSF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.218"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..257)
                     /locus_tag="OO335_004085"
     CDS             complement(<1..257)
                     /locus_tag="OO335_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004241.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            282..521
                     /gene="relB"
                     /locus_tag="OO335_004086"
     CDS             282..521
                     /gene="relB"
                     /locus_tag="OO335_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR6004242.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            521..808
                     /gene="relE"
                     /locus_tag="OO335_004087"
     CDS             521..808
                     /gene="relE"
                     /locus_tag="OO335_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR6004243.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            880..1035
                     /gene="hokD"
                     /locus_tag="OO335_004088"
     CDS             880..1035
                     /gene="hokD"
                     /locus_tag="OO335_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6004244.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1253..1504
                     /locus_tag="OO335_004089"
     CDS             1253..1504
                     /locus_tag="OO335_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004245.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1571..1849
                     /locus_tag="OO335_004090"
                     /pseudo
     CDS             1571..1849
                     /locus_tag="OO335_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1851..2909
                     /locus_tag="OO335_004091"
     CDS             1851..2909
                     /locus_tag="OO335_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6004246.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            2910..3275
                     /locus_tag="OO335_004092"
     CDS             2910..3275
                     /locus_tag="OO335_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6004247.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            3272..>3511
                     /locus_tag="OO335_004093"
     CDS             3272..>3511
                     /locus_tag="OO335_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6004248.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLV"
BASE COUNT          927 a          817 c          935 g          832 t
ORIGIN      
        1 gacatgctaa agcaaaaaat agatgaataa gttgagttgt gcatatgtag cctgaccgtc
       61 acaaagtata tggtgtctgt accagtaaga tgatggccgg actctttaaa aaagagctga
      121 cctgcacaat acaggatgga cttagcaatg gctgctcctg gcacaaagcg gacagtgatc
      181 accgttctta cgactacttt ctgacttcct tcgtgacttg ccctaagcat gttgtagtgc
      241 gatacttgta atgacatttg taattacaag aggtgtaaga catgggtagc attaacctgc
      301 gtattgacga tgaacttaaa gcgcgttctt acgccgcgct tgaaaaaatg ggtgtaactc
      361 cttctgaagc gcttcgtctc atgctcgagt atatcgctga caatgaacgc ttgccgttca
      421 aacagacact cctgagtgat gaagatgctg aacttgtgga gatagtgaaa gaacggcttc
      481 gtaatcctaa gccagtacgt gtgacgctgg atgaactctg atggcgtatt ttctggattt
      541 tgacgagcgg gcactaaagg aatggcgaaa gctgggctcg acggtacgtg aacagttgaa
      601 aaagaagctg gttgaagtac ttgagtcacc ccggattgaa gcaaacaagc tccgtggtat
      661 gcctgattgt tacaagatta agctccggtc ttcaggctat cgccttgtat accaggttat
      721 agacgagaaa gttgtcgttt tcgtgatttc tgttgggaaa agagaacgct cggaagtata
      781 tagcgaggcg gtcaaacgca ttctctgaac caaagcatga catctctgtt tcgcaccgaa
      841 ggtgacactt ctgctttgcg ttgacaggag aagcaggcta tgaagcagca aaaggcgatg
      901 ttaatcgccc tgatcgtcat ctgtttaacc gtcatagtga cggcactggt aacgaggaaa
      961 gacctctgcg aggtacgaat ccgaaccggc cagacggagg tcgctgtctt cacagcttac
     1021 gaacctgagg agtaagagac ccggcgagtg agaaatccct cgccacctct gatgtggcag
     1081 gcatcctcaa cgcacccgca cttaacccgc ttcggcggtt ttttgttttt attttcaacg
     1141 catttgaagt tctggatggt gccggaatag aatcaaaaat acttaagtag cgcgcaggga
     1201 taaaagggat ggaccccgaa caggggagtg ctacttatct ggaaggattc tgatgatgaa
     1261 catcgaagaa ctgcgtaaaa ttttttgtga agatggcctc tatgctgtgt gcgttgaaaa
     1321 tggaaatatt gttagtcatt accgcattgt gtgtttgcaa aaaaatgggg ctgcgttaat
     1381 taattttgtg gatgcccgag tgacggacgg atttatcttg cgcgacggtg agtttgtcac
     1441 ttcattacag gcattgaaag agatcggaat aaaagctggc ttttctgctt tttcagaaga
     1501 ataaactcat ctacaatctt gcgcggggct gaactcccgc tgagtaacac cgtgccaccg
     1561 gagaaaaccg atggcacgca acgcaaaata ttacaatcat gataattcga ccgttcttgc
     1621 ccacacgcac gagcggtatt ctcacgcatt taagtcagac tggtaccagc atccctcatg
     1681 cactgaagaa caggccgaat ggcttattca gtgttaccgc aggcgcggat gcgaggttaa
     1741 aaaagccctt agcctcgact accgtcacta gataatctcc gtcaggctcc cttactccga
     1801 acagccaccg cgtccgtccc gcacattcca gcaacggatc tggaggtaac gtgcgggtat
     1861 tacttcgacc tgttctggtt ccggaactcg ggctggtggt ccttaagccc ggtcgtgaat
     1921 ccatgccggt attccacaat acccgggtac tggtggagcc ggaaccgaaa agcatgcgta
     1981 atctgccgtc cggggtcgtt cctgccgttc gccagccgct agtggaagac aaaacattgc
     2041 tgccgttttt cagtaacgca cgggtgattc gtgctgctgg tggtgctggc gcattgtctg
     2101 actggctgtt gcgccatatt aaatcctgcc agtggccaca cggcgattat catcacagcg
     2161 aaaccgtcat tcaccgttat ggtaccggcg caatggtatt gtgctggcac tgcgacaacc
     2221 agctgcgtga ccagacatcc gaatcactcg agcaacttgc tcatcaaaac ctgtcagcat
     2281 ggatgattga cgtcatcggt cacgcaataa gcggtacgca ggagcgtgaa ttatctctgg
     2341 ctgaattatc ctggtgggcg gtccgcaatc aggtggcgga cgcgctaccg gaagcggtat
     2401 tacgtcgttc gctggggttg cgtgcggaaa aaatccgctc aatgtaccgt gaaagcgaca
     2461 tcgtgccggg agagcagacc gccaccagca tactgaagca gcgcacaaaa aatcttgcgc
     2521 cgctgcctca cgcccaccag caacagaatc caccacagga agagacggtg gtcagcattg
     2581 ccgttgatcc ggaatctccg gctcagtatc tccagcgcca gaaaccacaa cgggaagaga
     2641 tgcctgtata cacgcgctgg gtaaaaacgc agaaatgcat gacgtgtggc aatcaggcag
     2701 atgatccgca ccacatcatt ggtcatggac tgggagggat gggaacaaag gctgacgatt
     2761 tgtttgttat tccgctgtgt cgtaaatgcc ataacgaact acacgccggg gtaaaagatt
     2821 ttgaagaaaa acacggtagt cagttgttgt tgctgattcg ttttttaatg cacgcgagaa
     2881 attcgggtgt tctgaagtgg aaagcataaa tgaccgaacg catagaattt gttttgcctt
     2941 acccgccaac ggtgaacacc tactggcgac gtcgtggcag cacatatttt gtatcaaaag
     3001 ccggtgagcg ttatcgccgt gatgtggcgc taattgttcg ccagcagcga ctgaaattaa
     3061 acctgtccgg aaggctggcg ataaagatta ttgcagagcc accggataag cgccgtcgtg
     3121 acctggacaa tatcctgaag gcaccgctgg atgcgctgac gcatgctgga ctacttatag
     3181 acgacgagca gtttgatgaa atcaatattg tgcgcggtca gctcgttcct ggtgggcggc
     3241 tggggataaa aatcacagaa ctggggtgcg catgaataac cagtatttac agtttgtgcg
     3301 tgagcagctc attatcgcca ccgctgattt gagtggggca acaaaaggtc agcttgaagc
     3361 ctggcaagag aatgccatgt tcgatacagg gcgttacagg cgtaaaaaaa tccggtaccg
     3421 cgatgaagtg actggaaaaa tgataacgcg ggataatcca ccaatcccgg gaaagcaatc
     3481 gctggcgaag gggacgtcaa ttcctctggt c
//
LOCUS       DAJTSF010000213         3493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000213 DAJTSF010000000
VERSION     DAJTSF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.220"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..311
                     /locus_tag="OO335_004094"
     CDS             <1..311
                     /locus_tag="OO335_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6004249.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="OO335_004095"
     CDS             468..1217
                     /locus_tag="OO335_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6004250.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="OO335_004096"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="OO335_004096"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR6004251.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO335_004097"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO335_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6004252.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..>3493
                     /locus_tag="OO335_004098"
     CDS             3361..>3493
                     /locus_tag="OO335_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR6004253.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     "
BASE COUNT          961 a          908 c          863 g          761 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aac
//
LOCUS       DAJTSF010000214         3488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000214 DAJTSF010000000
VERSION     DAJTSF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.221"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..389
                     /locus_tag="OO335_004099"
     CDS             <1..389
                     /locus_tag="OO335_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR6004254.1"
                     /translation="HGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITEN
                     GSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGW
                     LTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="OO335_004100"
     CDS             complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="OO335_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6004255.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1072..2277
                     /gene="tet(B)"
                     /locus_tag="OO335_004101"
     CDS             1072..2277
                     /gene="tet(B)"
                     /locus_tag="OO335_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6004256.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="OO335_004102"
     CDS             complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="OO335_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6004257.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3116..3487
                     /locus_tag="OO335_004103"
     CDS             3116..3487
                     /locus_tag="OO335_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6004258.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
BASE COUNT          948 a          641 c          716 g         1183 t
ORIGIN      
        1 accatggcgt acatgccaaa gtttccacgc cagtgcattt acgaaaagca cgtacttgct
       61 atgatcattt agctggcgaa gttgccgtta agatctatga ttccctttgt caacagcaat
      121 ggatcactga aaatggttca atgatcacat taagtggtat tcaatatttt catgaaatgg
      181 gaattgacgt tccttccaaa cattcacgta aaatctgttg tgcgtgttta gattggagtg
      241 aacgccgttt ccatttaggt gggtacgttg gagccgcatt attttcgctt tatgaatcta
      301 aagggtggtt aactcgacat cttggttacc gtgaagttac catcacggaa aaaggttatg
      361 ctgcttttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
      421 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      481 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      541 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      601 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      661 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      721 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      781 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      841 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      901 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      961 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
     1021 tcattgatag agttatttta ccactcccta tcagtgatag agaaaagtga aatgaatagt
     1081 tcgacaaaga tcgcattggt aattacgtta ctcgatgcca tggggattgg ccttatcatg
     1141 ccagtcttgc caacgttatt acgtgaattt attgcttcgg aagatatcgc taaccacttt
     1201 ggcgtattgc ttgcacttta tgcgttaatg caggttatct ttgctccttg gcttggaaaa
     1261 atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt cattaatagg cgcatcgctg
     1321 gattacttat tgctggcttt ttcaagtgcg ctttggatgc tgtatttagg ccgtttgctt
     1381 tcagggatca caggagctac tggggctgtc gcggcatcgg tcattgccga taccacctca
     1441 gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa gttttgggct tggtttaata
     1501 gcggggccta ttattggtgg ttttgcagga gagatttcac cgcatagtcc cttttttatc
     1561 gctgcgttgc taaatattgt cgctttcctt gtggttatgt tttggttccg tgaaaccaaa
     1621 aatacacgtg ataatacaga taccgaagta ggggttgaga cgcaatcgaa ttcggtatac
     1681 atcactttat ttaaaacgat gcccattttg ttgattattt atttttcagc gcaattgata
     1741 ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa atcgttttgg atggaatagc
     1801 atgatggttg gcttttcatt agcgggtctt ggtcttttac actcagtatt ccaagccttt
     1861 gtggcaggaa gaatagccac taaatggggc gaaaaaacgg cagtactgct cggatttatt
     1921 gcagatagta gtgcatttgc ctttttagcg tttatatctg aaggttggtt agttttccct
     1981 gttttaattt tattggctgg tggtgggatc gctttacctg cattacaggg agtgatgtct
     2041 atccaaacaa agagtcatca gcaaggtgct ttacagggat tattggtgag ccttaccaat
     2101 gcaaccggtg ttattggccc attactgttt gctgttattt ataatcattc actaccaatt
     2161 tgggatggct ggatttggat tattggttta gcgttttact gtattattat cctgctatcg
     2221 atgaccttca tgttaacccc tcaagctcag gggagtaaac aggagacaag tgcttagtta
     2281 tttcgtcacc aaatgatgtt attccgcgaa atataatgac cctcttgata acccaagagg
     2341 gcatttttta cgataaagaa gatttagctt caaataaaac ctatctattt tatttatctt
     2401 tcaagctcaa taaaaagccg cggtaaatag caataaattg gcctttttta tcggcaagct
     2461 cttttaggtt tttcgcatgt attgcgatat gcataaacca gccattgagt aagtttttaa
     2521 gcacatcatc atcataagct ttaagttggt tctcttggat caatttgctg acaatggcgt
     2581 ttaccttacc agtaatgtat tcaaggctaa ttttttcaag ttcattccaa ccaatgatag
     2641 gcatcacttc ttggataggg ataaggtttt tattattatc aataatataa tcaagataat
     2701 gttcaaatat actttctaag gcagaccaac catttgttaa atcagttttt gttgtgatgt
     2761 aggcatcaat cataattaat tgctgcttat aacaggcact gagtaattgt tttttatttt
     2821 taaagtgatg ataaaaggca cctttggtca ccaacgcttt tcccgagatc tcatctattg
     2881 aaacagcttg atagcctttt tcaacaaaca atattcgtgc tgagttaacc agtgattgat
     2941 aggtactctt aaaattttct tgttgatgat ttttattttc catgatagat ttaaaataac
     3001 ataccgtcag tatgtttatg gtatcatgat gatgtggtcg tgacaatctt aagaacattt
     3061 aggttatttt atgtatattg aacagcattc tcgctatcaa aataaagcta ataacatcca
     3121 attaagatat gatgataagc agtttcatac aacggttatc aaagatgttc tattatggat
     3181 tgaacataat ttagatcagt ctttactgct tgatgatgtg gcgaataaag cgggttatac
     3241 caagtggtat tttcagcggc tgttcaaaaa agtaacaggg gtcacactgg ctagctatat
     3301 tcgtgctcgt cgtttgacga aagcggctgt tgagttgagg ttgacgaaaa aaactatcct
     3361 tgagatcgca ttaaaatatc aatttgattc ccaacaatct tttacacgtc gatttaagta
     3421 catttttaag gttacaccaa gttattatcg gcgtaataaa ttatgggaat tggaggcaat
     3481 gcactgag
//
LOCUS       DAJTSF010000215         3477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000215 DAJTSF010000000
VERSION     DAJTSF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.222"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            19..897
                     /locus_tag="OO335_004104"
     CDS             19..897
                     /locus_tag="OO335_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6004259.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="OO335_004105"
     CDS             894..2285
                     /locus_tag="OO335_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6004260.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="OO335_004106"
     CDS             2282..2539
                     /locus_tag="OO335_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004261.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="OO335_004107"
     CDS             2634..2834
                     /locus_tag="OO335_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR6004262.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="OO335_004108"
     CDS             2827..3207
                     /locus_tag="OO335_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6004263.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          912 a          755 c         1007 g          803 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaacac gctggtggtc
     3361 agatgagttt gcagatgtga tgacatatgg ttattattct gcctccggcc ctttagctca
     3421 gttggtcaga gcgagcgact cataatcgcc aggtcgctgg ttcaagtcca gcaaggg
//
LOCUS       DAJTSF010000216         3456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000216 DAJTSF010000000
VERSION     DAJTSF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.223"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(226..429)
                     /locus_tag="OO335_004109"
     CDS             complement(226..429)
                     /locus_tag="OO335_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004264.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="OO335_004110"
     CDS             complement(431..586)
                     /locus_tag="OO335_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR6004265.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="OO335_004111"
     CDS             complement(789..1196)
                     /locus_tag="OO335_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6004266.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="OO335_004112"
     CDS             1273..1500
                     /locus_tag="OO335_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6004267.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="OO335_004113"
     CDS             1484..1906
                     /locus_tag="OO335_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004268.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2761
                     /locus_tag="OO335_004114"
                     /pseudo
     CDS             1984..2761
                     /locus_tag="OO335_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2768..3456
                     /locus_tag="OO335_004115"
                     /pseudo
     CDS             2768..3456
                     /locus_tag="OO335_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          885 a          764 c          940 g          867 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg
     2461 tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg
     2521 catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat
     2581 acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag
     2641 catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct
     2701 ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta
     2761 acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc
     2821 gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga
     2881 aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat
     2941 tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt
     3001 gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat
     3061 gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc
     3121 ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc
     3181 tgatgttatg agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt
     3241 gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac
     3301 gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
     3361 cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acattgctcg gcgagcgggt
     3421 gatggatcgc atggtcatga acggcgggcg ctgggt
//
LOCUS       DAJTSF010000217         3413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000217 DAJTSF010000000
VERSION     DAJTSF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.224"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..237
                     /locus_tag="OO335_004116"
     CDS             <1..237
                     /locus_tag="OO335_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR6004269.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            247..885
                     /locus_tag="OO335_004117"
     CDS             247..885
                     /locus_tag="OO335_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6004270.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            888..2051
                     /locus_tag="OO335_004118"
     CDS             888..2051
                     /locus_tag="OO335_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6004271.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSHEGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2135..>3413
                     /locus_tag="OO335_004119"
     CDS             2135..>3413
                     /locus_tag="OO335_004119"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6004272.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          760 a          804 c          986 g          863 t
ORIGIN      
        1 ttgttaggtt acgttatccc attatccagt gcggcaatca atgcggtgag tattccagac
       61 tatttcgcct ggggcgggat tgcactggtg attcagttac tcgtttttgc tggtgtcaga
      121 ctttatatgc ccgcattaag cgaaaaaatt attaatcaca ataccgcagc aggaatgttt
      181 atgggaaccg ccgcgctggc tggcggtatt tttaacgcag cttgtatgac atggtaatgg
      241 acgatcatgg ccagaaaacg caaatcaaga aataacagta aaattggtca cggagcgatc
      301 agtcgcattg gtagaccgaa taatcctttt gaaccgcgtc gcaatcgcta cgcacaaaaa
      361 tacttaacgt tggcgctaat gggcggtgcc gcttttttcg tattgaaagg ttgtagcgat
      421 agtagcgatg tcgataacga cggcgacgga acattttacg cgacagtgca ggattgtatt
      481 gatgacggta ataatgccga catttgcgcg cgtggctgga acaacgccaa agcggcattt
      541 tatgccgatg ttccaaagaa tatgactcag cagaactgtc agtctaagta cgaaaattgc
      601 tactacgaca atgttgaaca gagttggatc ccggtcgttt ctggattttt gttaagccgg
      661 gttattcgta aagatcgcga tgagccgttc gtctataaca gcggcggatc ctcatttgct
      721 tcccgccccg tctggcgcaa cacttccggt gattactcct ggcgcttcgg ttctggcaaa
      781 aaagagtctt actcttcagg cggctttacc accagaaaag cgtcgaccgt ttcgcgcggc
      841 ggctatggtc gttcttccag cgcccgtggg cattggggag gctaatcatg ctgagacaca
      901 acgttcctgt gcggcgggat ctggaccaga tcgccgctaa caacggtttc gactttcata
      961 tcatcgacaa tgaaatctat tgggacgaga gtcgggctta ccgttttact ctgcgccaga
     1021 ttgaagagca gatcgaaaaa ccgactgcgg aactgcatca gatgtgcctt gaggtggtgg
     1081 atcgcgcggt taaagacgaa gagatcctga cgcaactggc gatcccgccg ttgtactggg
     1141 atgtgatcgc cgaaagctgg cgcgcacgcg atccttcgct gtatggccgg atggattttg
     1201 cctggtgtgg caatgcgccg gtgaagctgc tggagtacaa cgccgatacg ccgacctcgt
     1261 tgtatgagtc ggcttatttc cagtggttgt ggctggagga tgcccggcgc agcggcatta
     1321 ttccgcgtga tgccgatcag tacaacgcca ttcaggaacg cctgatttcg cgctttagtg
     1381 agctttacag tcgggaactg ttttattttt gctgctgtca ggacaccgat gaagacagga
     1441 gtaccgtgct gtacttgcag gactgcgccc agcaggcagg gcaggagtcg cggtttatct
     1501 acattgaaga tctcggtttg ggcgtcggcg gcgtactgac cgatcttgat gataatgtca
     1561 tccagcgtgc atttaagctg tatccgctgg agtggatgat gcgtgacgat aacggtccgc
     1621 tgctgcgcaa gcgtcgcgag caatgggtgg agccgttatg gaaaagtatc ttgagtaata
     1681 aagggctaat gccgctgctt tggcgcttct tccctggtca tcctaacctt cttgcatcct
     1741 ggtttgaggg tgaaaaatcg cagattgccg ctggcgaaag ctatgtgcgt aaaccgatct
     1801 actcgcacga aggcggaaac gtcaccattt tcgacggtca gaataacgtc gttgaccacg
     1861 ctgatggtga ttacgccgat gaaccgatga tctaccaggc gtttcaacct ctgccgcgat
     1921 ttggcgatag ctacacactc atcggtagct ggattgtcga tgatgaagcg tgcggaatgg
     1981 ggatccgtga agataacaca ctgatcacca aagacacctc acgtttcgtt ccgcattaca
     2041 ttgctggata agaatgtttt agcaatctct ttctgtcatg aatccatggc agtgaccata
     2101 ctaatggtga ctgccattga tggagggaga cacagtgcac tggcaaactc acaccatttt
     2161 taatcaacct ataccattaa ataacagcaa cttatacctg tctgatggcg cgctttgcga
     2221 agcggtaacg cgtgaaggtg ctggctggga tagcgatttt ctagccagta ttggtcagca
     2281 gttaggaacg gccgaatccc ttgaactggg gcggctggcg aatgtaaacc cgcctgaact
     2341 gttgcgctac gatgcccagg ggcggcgtct ggacgatgtt cgtttccatc ccgcatggca
     2401 cctgctgatg caggcgttat gtaccaatcg ggtgcacaat cttgcctggg aagaagacgc
     2461 tcgctccggc gcatttgtgg cgcgcgcggc gcgttttatg ttacatgcgc aggttgaggc
     2521 tgggtcgtta tgtccagtaa cgatgacctt tgccgccacg ccattgttgt tacagatgtt
     2581 acccacgccg tttcaggact ggaccacgcc gctgctgagc gatcgctacg attctcactt
     2641 attgccaggt gggcaaaaac gcggtttgtt gattggcatg ggaatgacgg aaaagcaggg
     2701 cggttccgat gtcatgagca acaccacccg tgcagagcgt ttggaagatg gctcttatcg
     2761 gctggtgggg cataaatggt ttttctcggt gccgcaaagc gatgcgcatc tggtgctggc
     2821 gcagaccgcg ggcggtctgt cctgcttttt tgtgccgcgc tttttgcctg acgggcaacg
     2881 caacgcgatt cgcctcgagc ggctgaaaga taagctgggt aatcgctcta acgccagttg
     2941 cgaagtggag tttcaggatg ccattggctg gttattgggg caggaagggg aaggaattcg
     3001 tctgatcctg aaaatggggg ggatgacgcg ttttgattgc gccctgggta gccatgccat
     3061 gatgcgccgt gcattttcgc tggcgattta tcatgcacat caacgccatg tttttggtaa
     3121 tccattgatc caacagcccc ttatgcgtca tgtcttaagt cgcatggcac ttcagcttga
     3181 agggcaaacg gcgttgctgt ttcgtcttgc gcgagcgtgg gaccggcgtg ccgatgccaa
     3241 agaagccctg tgggcgcgtt tatttacgcc tgcggcaaaa tttgtgatct gcaaacgagg
     3301 tatgccgttt gtggccgaag cgatggaggt gctgggcggc attggttatt gcgaggagag
     3361 cgagctgccg cggctttacc gggagatgcc ggtaaacagt atttgggaag gtt
//
LOCUS       DAJTSF010000218         3366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000218 DAJTSF010000000
VERSION     DAJTSF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.225"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            147..550
                     /locus_tag="OO335_004120"
                     /pseudo
     CDS             147..550
                     /locus_tag="OO335_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1134..1850
                     /locus_tag="OO335_004121"
     CDS             1134..1850
                     /locus_tag="OO335_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004273.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2402..2806
                     /locus_tag="OO335_004122"
     CDS             2402..2806
                     /locus_tag="OO335_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004274.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2869..3366
                     /locus_tag="OO335_004123"
                     /pseudo
     CDS             2869..3366
                     /locus_tag="OO335_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          945 a          697 c          780 g          944 t
ORIGIN      
        1 gaagcactga acagcaagat caggctgctg agaataaaag ccaagggcta ccgcaaccgg
       61 gagcgcttta agctgggagt gatgttccac tatgggaaac tgaatatagc gttctaagcc
      121 ttcccaccat gatcggggaa gccccagata tgaacaggca cattcagagt tactttaaag
      181 agcttactga acgtctgagg tgttagagcc atgactgccg cagcattgct ggcggaaatg
      241 cctgaatcga gcagcctctc gagacgtgaa atcagcgcac ttgtcggtgt tgctcaggta
      301 aaccgggact cgggaactct gcgcggtcga cgaaccattt tcggtgattg tgctggggaa
      361 gaacagctct gtacatggcg gcgcttgagg ccccccccgg tttaatctgg tgataaaggc
      421 gttttacatg cgcctacttg cagccggaaa cgccaaaaaa gtggcactgg ttgcctgtat
      481 gcgtaagttg ctgaccatta tgaacgcaat gctcagaaaa aacgaagaat ggaatgaatc
      541 gtatttatga aaactggatt gtgggccata aatattccaa aaagatagcc cgctccgttt
      601 attaccggaa cgggcgagag catgacattg tctgttatta taatgagact gctgattcta
      661 cttattttta atttcaagaa aagcgttttc tacaaagaat tatgagattt aatgttttgt
      721 ttagacaata ttatcattct gttcaataat gaaagtcttc atcaacaaca agtaaaaaca
      781 acgtccctct catcgaaccg gacttgcaga tttcccgcat ccggctctct tgcaacacat
      841 cagaccttca cgttcgggcg gttatgggga aggtccagca agctgtatga gccctaactg
      901 tttgaatacc agtagattac cagaaacttc tatcgattga agacattacc aactagcgtt
      961 actaaatgtt gaccaagttt tgttcacttg tctggttttg atagtagcat tctgcagagt
     1021 attttgatat gataaatatg cattaacaat gatattattg ttaatttcct agcatgaaaa
     1081 ttaaaagatc aacttttatc tcgaatattt tttacattat cagttagttt cttatgaatg
     1141 acaattcatt gcttagaaat tcttcattat tcatcgccta tatgggatgt gtagggtggg
     1201 tcagtgctta ttcttatggc tggggaacct ctttttacta tggatttcca tggtgggttg
     1261 ttggtgcagg tttggatgat gttgccagaa gtttactcta tgctatcata gtaatgggta
     1321 tactttttac cggttggggg attggtattt tattttttct tcttataaag aaaagaagta
     1381 aaatacaaga cttgagtttt tttagactat tttttgctat taccctactg tttttccctg
     1441 taatattcga acttttaatt ctcaaacaat atttcatact tccattatca ctttcattca
     1501 ttattagttc attggttatt tcaataataa ttcgtatata tggacggatc ttttctgtat
     1561 catgtttttc agatatccct tttgttagag aacaccgcat taagttaatt atggctggtt
     1621 ttttggttta tttttggttt ttttcctttc ttgttggctg gtataaacct caattaaaaa
     1681 aagaatatca gatgctttgt tataataata gttggtatta tgttcttgct cgttatgatt
     1741 cacggctcgt tttatctagt tcatttaaag atgacagtaa cagattctta atatttaata
     1801 cagaacagtc gggattttat gaaatcaatg atgtttatgt cagaaagtaa taatcctctc
     1861 ccacaaagtg aattaaatga atatataatg cgttcgaagt atatgtattg agctgtcata
     1921 ctgagaaaca aaccttaggg cagcacaatt aaagccagaa acaactatgc aagaaagtta
     1981 ctaaataagt gctcggaaca gtaaaacact caggggggac tcagtccgga ttatgtaatc
     2041 agataaattg ccaaacatca caaatcacca gccggactga gtgatgccga gcattgcacc
     2101 aataccccgt gatgaacgat gcctgatgca gaaagctccc tataaaacaa agaacaaaaa
     2161 ctacgaccgg cgattcaccg tcatactgat gctgcatcgt ggtgacactg tctgtcactt
     2221 tcatgttgac tgaacaactc tgctgcgaag aaggtgtacc gccggcggga tttgattgtc
     2281 cggcgtctag cttccacttc cctgatggaa ctgaaagttg ttatggccgg tcagcttgcc
     2341 ctgtccgcag gacatgaggt gctgtgacgg acagcaccag taacggaaag tgacaatgaa
     2401 aatgaatact gcgctgatag ttgcactaat gtgtatgtgg tatgcggtac ccgctgctgc
     2461 aaaagagact ttactggcta tgccacgtaa ttcaacagaa cattgctatg cggagattaa
     2521 tgtgcacgga ccatatgggg tgtatttccg agtggtcccg catccccccg ggggaaagag
     2581 ctgggtcgaa tgtaacagtg actactatta ctctgataaa ccaccgggag tacagatact
     2641 cggaacccgt gcagggtgtc gggtatacgg aatctgcggg accacctcga cactgcatgt
     2701 ggctggaaga ggggttgtgt gtattaaaaa tatatgcagt ccacgtggga tgataattca
     2761 caggatacgg aaacgtcctg tagtggctgt aagcgatgag atgtgatctt acccagcaat
     2821 agtagacacg cggctaagtg agtaaactct cagtcagagg tgactcacat gacaaaaaca
     2881 gtatcaacca gcaaaaaaac acgtaaacag cattcgcctg aattttgcag tgaagccctg
     2941 aagcttgctg aacgcatcgg tgttgctgcc gcagcccgtg aactcagcct gtatgaatct
     3001 cagctctatg cgtggcgcag caaactgcag caacagatga cctcgtctga gcgtgaaagt
     3061 gaactgcccg cctgaagcgg caactggcgg agcaggcaga ggaactggct atcctccaaa
     3121 aggccgcgac atactttgcg cagcggctgg tatacgtggt gtcagcgtcg gacaggaata
     3181 agccctcgtc agcagttccg tcagcactgc gacagcgttg tcctcgcggc ggcctttacc
     3241 cggtcaaaac agcgttacgg tgcaccacgc ctgacggatg aactgcgtgc tcagggttac
     3301 cactttaacg taaaaaccgt ggcggcaagc ctgcgccgtc aggggctgag ggcaaaggcc
     3361 tcccgg
//
LOCUS       DAJTSF010000219         3351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000219 DAJTSF010000000
VERSION     DAJTSF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.226"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            24..100
                     /locus_tag="OO335_004124"
     tRNA            24..100
                     /locus_tag="OO335_004124"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="OO335_004125"
     tRNA            143..218
                     /locus_tag="OO335_004125"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
     gene            402..3305
                     /locus_tag="OO335_004126"
     rRNA            402..3305
                     /locus_tag="OO335_004126"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          892 a          728 c         1023 g          708 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
      361 caacacgatg atgagtcgaa agaaacatct tcgggttgtg aggttaagcg actaagcgta
      421 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      481 ggtaaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      541 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      601 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      661 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
      721 atacagggtg acagccccgt acacaaaaat gcacatactg tgagctcgat gagtagggcg
      781 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      841 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      901 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      961 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
     1021 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
     1081 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
     1141 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
     1201 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
     1261 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
     1321 accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
     1381 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
     1441 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
     1501 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
     1561 gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt
     1621 gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg
     1681 ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg
     1741 tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc
     1801 tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg
     1861 tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaatcaa ggccgaggcg
     1921 tgatgacgag gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta
     1981 agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc
     2041 aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag
     2101 aaggcacgct gatacgtagg tgaagcgact tgctcgtgga gctgaaatca gtcgaagata
     2161 ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta
     2221 tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggttagcg caagcgaagc
     2281 tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat
     2341 tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac
     2401 ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa
     2461 agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata
     2521 ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc
     2581 tttaatgttt gatgttctaa cgtggacccg tgatccgggt tgcggacagt gtctggtggg
     2641 tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat
     2701 cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac
     2761 ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat
     2821 cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat
     2881 cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc
     2941 caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag
     3001 acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg
     3061 agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg
     3121 tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat
     3181 gagttctccc tgacccttta agggtcctga aggaacgttg aagacgacga cgttgatagg
     3241 ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta
     3301 accttacaac gccgaagatg ttttggcgga ttgagagaag attttcagcc t
//
LOCUS       DAJTSF010000220         3325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000220 DAJTSF010000000
VERSION     DAJTSF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.227"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            454..1281
                     /locus_tag="OO335_004127"
     CDS             454..1281
                     /locus_tag="OO335_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6004275.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1325..2074
                     /locus_tag="OO335_004128"
     CDS             1325..2074
                     /locus_tag="OO335_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6004276.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2071..2640
                     /locus_tag="OO335_004129"
     CDS             2071..2640
                     /locus_tag="OO335_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004277.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2764..3006
                     /locus_tag="OO335_004130"
     CDS             2764..3006
                     /locus_tag="OO335_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6004278.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3010..3156
                     /locus_tag="OO335_004131"
     CDS             3010..3156
                     /locus_tag="OO335_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004279.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3165..>3325
                     /gene="xisR"
                     /locus_tag="OO335_004132"
     CDS             3165..>3325
                     /gene="xisR"
                     /locus_tag="OO335_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR6004280.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          928 a          797 c          854 g          746 t
ORIGIN      
        1 acgccatcct gacaagcgtt ttatcttcaa cgggaatgag atatggaaac gtggagaatc
       61 aaccacagag tatacccgcg cacaccagcg aacaatcaga cgactgcgca gactcatcgc
      121 caggaaacag tatacagaaa aatggttcag aaaatatgac acatggagcg ccggatatta
      181 cgaactgatg gcaacaaaac cattctgacg taaacgaaat taaccatgac gcaattaaat
      241 aaggcaagcc gaatacatca ggaggaccat gaacatttat ttcagaatag ttatatcact
      301 ggcaattatc gcatgtattt acggattaat cgttccggcc ctcatatcaa tgaaggatac
      361 ggtagcagtg atttctggct ttgctctggc gtgtctgacc ccgccctgca tttatgccat
      421 ttataagggt ctttcttttt ctaaggataa aagatgaaaa aagttattcc tgctttagcc
      481 cttgttctgc tgaccaccgg acttgtcggt tgcgatcgcg ttgagcctgg taatgttggc
      541 atcaaggtaa acaaactggg cgacgataaa ggcgtcggag aagtggtcgg tgttgggcgc
      601 tactggacgg gatggaacac tgaggtttac atcttcccga ccttcaaaca aatgaagacc
      661 tacgatgagc cattcagctt ccagatgagt gacggtacaa ccatcggcta tcacatcggc
      721 gtggcctaca aagttgatcc atccaaagtt accacagtct ttcagaccta ccgcaaaggc
      781 gtggacgaca ttaccgacac cgacctgcgc cagaagatag ccgacgcact caaccgactg
      841 gccagcaaaa tgaccaccga caaatttatc gacggcggca agtctgagct gctggatgca
      901 gctcttaaag acattcagga agaaatgacg cccatcggta ttcaggtaat gagcctctca
      961 tatgtgggta agccggagta cccgcctact gttatcgaca gcattaatgc caaagtcacg
     1021 gcgaaccaga aaaccctgca atgcgagcag gaagtaaaac agcgcgaagc ggaagccaac
     1081 atgttgcgcg cggaagctgc cggacaggca gatgcgattc gcacaaaagc ccaggccgaa
     1141 gccgatgcta ttcgtttacg cggtgaagct ctgcgccaga acccaaacgt tatggagctg
     1201 gaagccatca acaagtggaa cggtacactg ccgcaataca tgaccagcgg tgccaacaca
     1261 ccatttattc aggttaagta atacatatgc ccggtattac acgccgggct gtctggagat
     1321 aaaaatgaat actgtaacca tcaacaacaa acagtttccg gtaatcgaat atcgcggtca
     1381 gcgtgttgtg acgctggcga tgattgatga agtccaccag cgaccggaag gaaccgcgcg
     1441 tgcagcattc aaccgaaacc gtgagcattt catcagcggt gtggattatg ccgaattagg
     1501 tgcggacgta atacgtacgg acctcccgga aggaacattc tctaaatttg caccatcggg
     1561 gattgtactt tttgaatcag gttacctgat gttgacgaag ccattcaatg acgcccttgc
     1621 ctggcaggtt cagcgcgaac tggttaacag ctacttccgc actcgcacac cgctaacgga
     1681 aatcgagatg atcgctgcga tagccgccga cgctgttcgt caacagaagc gcctgaatca
     1741 tgtggaagaa cagctcgaaa cagtcacaga agcggtggaa accatcaaac gcggaaatat
     1801 gcgcgccgga tatgtcggtt accgccaggt ggtagccaaa agtggaatga gtgacaccaa
     1861 gtgtcggaat ctggtgaacg catatcaaat tcctacagac acccacgaat tcatgactcc
     1921 ggacggtctg ttgtctcgca gggctatcgt cgagcttgag ccatttatgg cggcgttcca
     1981 ccaaatgatg tcagaggctg acccacgcgg cacacgctgg tatcacccga agatggggct
     2041 tttccaggtg atcggatggg aggataaagc atgatcatcc agtcaaaact tattcgcgcc
     2101 gctctggtgt gcgctgctaa aaacgacgtt cgttattacc tgaacggtct tcacatcacg
     2161 ccgaaacata ttgaggcaac caatggttcc gtggcactgc gcatggctca cggcatccgg
     2221 acgaagaaaa acatcattgt ccagttcgaa gggggcgtcc cggccaaagc cgaaacgaca
     2281 gagctgattt ttagtaaaga gccgatcgct gttcatcgcg accagtttca gcgacgactg
     2341 tccattaccg gcattaaact ggtggacggt tgttttccgg atttggatcg catcattccg
     2401 aaaaagtttg accgctgtac tcacccggtg ctccaggcgg gttacctgag ttatccagag
     2461 aagatgtttg gtcgtgagcg taaatttatt cccgtccagt tacgtccctc cggtgacggg
     2521 caagcggtca gaattcagtt tgattccatc atcaactcaa tgtatggcaa tcctgaattt
     2581 gttgtgatgc cttgtcgtga tcatggcgat ttcaacgtgg ctcaggagca tccggaatga
     2641 aaatcgaata ccaggacgcc gcaggaggtg aatcaatgag ctggcctgat gcaatcgtaa
     2701 ctctggggat ggtattcgca gtagcgtttg ttgtgtactc gatttgtcga tggggataac
     2761 cacatgttcg ctttgattca acgcgggcag atatacacag acagagccgg atacccagta
     2821 gaaattactc gcagtactga gcactcagtg ttctttcgga ggatggacgg acgtaccggg
     2881 cgagtacgca tcggtgaatt cagtagcctg ttcgagcata ttgaccacct ggaatatcac
     2941 aagattctgg ctgaaacaga acaagaaaag cacctgaaga aattacgcgc aatgcaaagg
     3001 aagtaaaaaa tgaataaagc gtttgagcta tgggtccacc agcgttacgg caaccactac
     3061 gacctgacgc gagatgttga cggtttctac tgtcgtgaaa ttgtgaaacg aatgtttgag
     3121 gtctggtgcc actgccgtgg gctgagtgtt gtgtgaggta atgcatgggc aatgtgattc
     3181 aactggctcc caatgaatgg gtttgtgaaa gcgttctaat cgcaattacc gggctcaaac
     3241 caggcacaat tcttcggacc cggaaagaat gctggatggt tggaagagag tatattcacg
     3301 tatcaccaga cggtaatcca aagcc
//
LOCUS       DAJTSF010000221         3187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000221 DAJTSF010000000
VERSION     DAJTSF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.228"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            168..1271
                     /gene="proB"
                     /locus_tag="OO335_004133"
     CDS             168..1271
                     /gene="proB"
                     /locus_tag="OO335_004133"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6004281.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1283..2536
                     /gene="proA"
                     /locus_tag="OO335_004134"
     CDS             1283..2536
                     /gene="proA"
                     /locus_tag="OO335_004134"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6004282.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2651..2726
                     /locus_tag="OO335_004135"
     tRNA            2651..2726
                     /locus_tag="OO335_004135"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2684..2686,aa:Thr,seq:cgt)
     gene            complement(2741..>3187)
                     /locus_tag="OO335_004136"
     CDS             complement(2741..>3187)
                     /locus_tag="OO335_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6004283.1"
                     /translation="ESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGT
                     KAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWE
                     SIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
BASE COUNT          752 a          807 c          877 g          751 t
ORIGIN      
        1 tggtaattta ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc
       61 gcgcaacaaa acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa
      121 aacgttcgtt tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc
      181 agacgctggt ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc
      241 gtgcccatat cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga
      301 ttgttattgt gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaac
      361 tgccagcgac tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc
      421 aactgtggga acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc
      481 gtgctgatat ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc
      541 tcgataacaa tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta
      601 aggtcggcga taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac
      661 tgttgctgct gaccgatcaa aaaggtttgt ataccgctga tccgcgcaga aatccgcagg
      721 cagaactgat taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca
      781 gcgtttcagg cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt
      841 gccgtgcggg tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg
      901 tgatggaagg catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc
      961 gtaaacgctg gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa
     1021 ctgccgccat tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg
     1081 gcaatttctc gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc
     1141 acggtgtcag tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag
     1201 aaattgatgc aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga
     1261 ttacccgtta aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc
     1321 gtataaatta gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga
     1381 tgaactggaa gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc
     1441 gcgagccaat ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct
     1501 gaaaggcatt gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt
     1561 aatcgatggc ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg
     1621 ggttattggc gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg
     1681 cctgaaaacc ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc
     1741 ggcaacggtg gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt
     1801 gcaggcgatt gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata
     1861 catcgacatg ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc
     1921 gacaatcccg gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagtgc
     1981 agagatcgct gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg
     2041 taatacggtt gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt
     2101 aagcaaacaa atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca
     2161 gttgcaggca ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt
     2221 tctgtcatta gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat
     2281 tcgtgaacac ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca
     2341 gcgttttgtt aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac
     2401 cgacggcggc cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc
     2461 gcgtggccca atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta
     2521 caccattcgt gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc
     2581 attgacgcat cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg
     2641 tcctttcagg gccgatatag ctcagttggt agagcagcgc attcgtaatg cgaaggtcgt
     2701 aggttcgact cctattatcg gcaccattta aatcaataag ttacacatca ttagtacctt
     2761 ccttatttct tgactgggac aaatttggga ccgatgggtt cagaatcaag tctatttgcc
     2821 gtgcgtgttc ggtaaggtga ttaggtgcga ggtgagcata tcgacgaacc atttcgatag
     2881 actcccagcc tcccatttcc tgtaacactg ataacgggac tccggcttga accagccaac
     2941 ttgcccaggt gtgtctcaag tcgtgaaatc tgaaatcatc aataccagcc cgtctcagcg
     3001 ccgctttcca ggctgtgttt gcgtcatacc gcatcttcct tactgttggc gctttcgttc
     3061 cgtctggttt ggtacagctt tccttgtaca caaataccca acggtgatga tttccgattt
     3121 gttttttcaa tacgcgacat gcagtatcat tcagcgcaac gccaattgcg cggtttgatt
     3181 tactctc
//
LOCUS       DAJTSF010000222         3179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000222 DAJTSF010000000
VERSION     DAJTSF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.229"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="OO335_004137"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="OO335_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6004284.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="OO335_004138"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="OO335_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAJTSF010000223         3112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000223 DAJTSF010000000
VERSION     DAJTSF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.230"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO335_004139"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO335_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6004285.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="OO335_004140"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="OO335_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO335_004141"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO335_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR6004286.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO335_004142"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO335_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR6004287.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="OO335_004143"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="OO335_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          774 a          753 c          756 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccatccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAJTSF010000224         3099 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000224 DAJTSF010000000
VERSION     DAJTSF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.231"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(106..1815)
                     /locus_tag="OO335_004144"
     CDS             complement(106..1815)
                     /locus_tag="OO335_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR6004288.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2247..2966)
                     /locus_tag="OO335_004145"
     CDS             complement(2247..2966)
                     /locus_tag="OO335_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR6004289.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          953 a          588 c          519 g         1039 t
ORIGIN      
        1 ttgccaattg aggtggttgc ctgaaagttg gcagttaccc ggtcactaca ccttgttgga
       61 gtgaacatat caaaaacagg taattacaca atttaaatta cagtctcata aaaaatgatt
      121 aaatctattt attatttcaa taaacgtttt tttcatcttc tttgaaaatt gttctgctat
      181 agtatccggt tccagattgt tctcttttat tattctacaa tactctaata ttgtttcttt
      241 gtctctattt actacagata caaatacacc aggaatacca tctttattag ttgcggacag
      301 catttcgacc aatcgtcttg tggtaaaact atagtttttt agaatattac aaaaggcacg
      361 tattgtttcg gtatggccat tataaaatgc cataaaaagt cctgatgtac cgttgggatt
      421 ttttatccat aataattcat ctatatattc ctggtgaggg gggagtattt ttagtatatc
      481 tccatacgcc ataatggcct ttgtgtgccc attttgaagt gccataaaca acccatgctt
      541 tactttcaat aattctttaa tttgttcact actaagcatt ttcttattaa gcagacttcc
      601 gaacatacga atactatctg catgtccatt ctgcaatgca aaaaataacc caggtaaatc
      661 atggctgtct ttcgcagcta atagatataa tctcttatcg gaagttaacg gggttttaaa
      721 gactatttcc ccataggctt ttattgtgtc tacatgtccg ttcataaaag acatgcataa
      781 acccggcaca ttgtttgccc ctaccgcagc aagcaatgta agtattgttt cttgactaag
      841 attgcacata tttaatatct taccatatac ttgaataatg tctgaatatc cgttttgaag
      901 tgctatataa aatgcaggag taccatcaga gcttctcgtg gccagaaggt tcaccagttt
      961 agattgagaa atttcaccat ttttgtattt ttccatcagt ataaataata cttctgtcag
     1021 attctttgtt atcgcatgat atataactct ggaattgatc aattcatctg gtaaaagttt
     1081 cactgtgttt gttagagggg aattatgcct atcttcaaat attacctgcc cgggttgctc
     1141 cagaccataa attttccaat aaaaatcatc aaaaaaatcc ttagcggata attgactcag
     1201 ttcgaaatgg ctatcacaag taaatacagt acgtttatga gtaacagttc gattaggatc
     1261 gaaaaactga acaacccatt tttcactttc ttgtacgcgc tttattttca accctaacgc
     1321 catttggtgg tttaatgtgc atgctgataa tgttctgaca aagtctccat tttttttcat
     1381 gttataaaaa aaattagtaa taactgttcc ccacttttta ttttccacaa aataaatata
     1441 agctggtgat tttgaaaaat cttccgtttt ttccaaacat acattttttg caatagcatc
     1501 aggggtgcta agaagctgat agtttacctt cccatttgtt tccataaaat tcatttccca
     1561 ataggaagct aggtgacggc aaactatttg tcttccatat atatcttttg ccactccatt
     1621 tatatttacc tccacatttc tcttttctga aaaataaggt gctgttttat tcttaaactg
     1681 aattagttca ctggagtttg agaccctcat aatagaactt gttattgttc tattatctct
     1741 gttaacagac atatctgttg ataacgaatt ttttgttgaa aatatactag atgaaaatgt
     1801 tttatttaac ggcatagaag acaaccatag agatgttata atactattag attgcagtta
     1861 ctaatagtaa aagcctcctt acaaggaggt agcttattca gaatgctcct ataagatagc
     1921 gagctcgtca acgatacaga tgatctgatt atattgtgca aaaggagaac aggaaaatag
     1981 gcactggaaa agatgaaggc ctgatgggag cattaactaa tgcaaaatcc gacaggttaa
     2041 tatcccgaaa agcaatcaaa cgactcaaac aggagatgca cgctgaaatg gtggacaact
     2101 gttcggattt cagatgagta gctcagcagt ttatgagccg aatgctctgt cacgggcagg
     2161 aatcaggctg catacgcagt gccaaatcta atcgtagtta ttctaaccag ttttcaagct
     2221 acaaggtggt gtagctggca tcttctctac tctatcatca aacgataaaa tggttcctct
     2281 cgtaacattt gctcttgcct ttcacttcca tctcgatcgc ttcgtagttc attacgatag
     2341 ctgacatatt tccagtcttc aggtctaaca tctgacgccg gatactcccc gggagcgatt
     2401 ttgcttctta atagattaga ctcgagacac attataaatt gtcgtatttt atgaagtaat
     2461 aaagcactat attgcgagca ttcctgatct gattttacat atagcgtaaa ctgagctcct
     2521 atccccacac gagattgctg agaaactcga ttcatatcag ttatcttcca tttatctata
     2581 ggactgtcct ctgaaaataa taaaccagac aaaatttgga atgccagagg aacttgctcc
     2641 ctagctatac taatatgaaa cttgtcccca acaaaatccc ccttactctg aggattttca
     2701 cgacatgcat gaataaacac atctccacta tggttcacaa tctggaatcc ttggtacatg
     2761 acagaaaaag attgacatat atcagagtaa gttggcacgt tcgatgctac tttgaaatag
     2821 tacgctgggt attgccgtat ttggttgtac aatatattga agttattttt caatctttca
     2881 ttcgcagaaa gcatctgcgg tatttcagac ctcacaacat tcaaagaatc taaatttagt
     2941 tttagacagg gcttttttat gggcatagaa aacgtcctct ataaaataga tatattatct
     3001 tatattgatt gtttatcatc ttttataaaa cgatacatta tatgtaatgt atgtaagcgt
     3061 aagggtcctc tccgaacatg gtgggaaggc ttagaacgc
//
LOCUS       DAJTSF010000225         3069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000225 DAJTSF010000000
VERSION     DAJTSF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.232"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..498
                     /locus_tag="OO335_004146"
     CDS             <1..498
                     /locus_tag="OO335_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6004290.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="OO335_004147"
     CDS             578..1273
                     /locus_tag="OO335_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6004291.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="OO335_004148"
     CDS             1302..>3069
                     /locus_tag="OO335_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6004292.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAJTSF010000226         3065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000226 DAJTSF010000000
VERSION     DAJTSF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.233"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..197
                     /locus_tag="OO335_004149"
     CDS             <1..197
                     /locus_tag="OO335_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6004293.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="OO335_004150"
                     /pseudo
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="OO335_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="OO335_004151"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="OO335_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR6004294.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..2576
                     /locus_tag="OO335_004152"
     CDS             1821..2576
                     /locus_tag="OO335_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR6004295.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            2977..>3065
                     /locus_tag="OO335_004153"
     CDS             2977..>3065
                     /locus_tag="OO335_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6004296.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVF"
BASE COUNT          798 a          683 c          726 g          858 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaacta
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg ctgcacagta actttatttg tttaattaat acctaaccag
     2581 atgaaaaatt tgctcaaaat ttttaaatta acttattcaa cagtcacgca ttatattaaa
     2641 taacatttga ctgggttgaa cataacgcca atagcaaaag gagttatgtt caacaattta
     2701 attttagata tacatataac ttgttctatt aataaatcac ctcgtattct gactcggccc
     2761 ctggcgcagc aaagtcaggc atttatactc tttttaacac atagaaaatt catatcaata
     2821 taataaaaac ataaaatatt tgcatataca tataatctta aagtttagtc tatttaatgt
     2881 tcaatgaaat atttctacct gtataatctt taaagatgtt gaacatatat tcacattaaa
     2941 tatgattatg tacttgttac aaggataagg ttataaatga ataaagttac aaaaacagct
     3001 attgtgggct tacttgcgct tttcgcaggt aatgccgctg caaccgatgg tgaaatagtt
     3061 tttga
//
LOCUS       DAJTSF010000227         3051 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000227 DAJTSF010000000
VERSION     DAJTSF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.234"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..242
                     /locus_tag="OO335_004154"
     CDS             <1..242
                     /locus_tag="OO335_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004297.1"
                     /translation="FFDVYPRYRYEDIDVLDFRVCYNGEWYNTRFVPAALVEAILNSP
                     RVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            406..1956
                     /gene="pdeB"
                     /locus_tag="OO335_004155"
     CDS             406..1956
                     /gene="pdeB"
                     /locus_tag="OO335_004155"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR6004298.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(1998..2351)
                     /locus_tag="OO335_004156"
     CDS             complement(1998..2351)
                     /locus_tag="OO335_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR6004299.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(2471..2567)
                     /gene="ffs"
                     /locus_tag="OO335_004157"
     ncRNA           complement(2471..2567)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO335_004157"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2730..3041
                     /locus_tag="OO335_004158"
     CDS             2730..3041
                     /locus_tag="OO335_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR6004300.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
BASE COUNT          760 a          781 c          736 g          774 t
ORIGIN      
        1 tctttttcga tgtctaccca cgctaccgct atgaagatat cgacgtgctg gatttccgcg
       61 tttgctataa cggcgaatgg tacaacacgc gctttgtacc tgccgcgctg gttgaagcca
      121 tcttgaactc tccgcgtgtc gcggatgttc ataaggaaca actgcaaaaa atgatcgtcc
      181 gtaaaggtga actgtctttt tacgatattt ttaccctcgc tcgcgccgaa tcaacatctt
      241 aagttagggt tacataccag gcgtaaagct ctgcgcctgg tcaaatgaca atgatcgttt
      301 ccacccatca cttcatgaaa taccagctct acctccttat ctcctgccag cctttttcca
      361 caatcagata tactttccct acactgtgtt aataaggata tgctggtgag aacacgacat
      421 ctggtcggcc ttatttcggg agtactgatt ctttcagtat tgctgcctgt cggcttaagc
      481 atctggctgg cccatcagca ggtagaaaca tcgtttattg aagagctgga tacctattcc
      541 tcccgcgtcg ctattcgagc caataaggtg gcgacacaag ggaaagatgc gctgcaggag
      601 ctggaaagat ggcaaggcgc tgcctgtagc gaagcccatc tcatggaaat gcgtcgggta
      661 tcttacagtt atcgctatat tcaggaagtg gtttatatcg ataacaacgt tccccagtgt
      721 tcgtctctgg agcatgaaag tccgcccgat acctttcccg agctaggtaa aatttcgaaa
      781 gatggttatc gtgtctggtt aacatcgcat aacgatttag gcattatccg ttacatggtc
      841 gccatgggaa cggcacatta tgtcgtcatg atcgaccccg cttcctttat tgatgtcatt
      901 ccctatagtt catggcaaat tgatgccgcc attattggca atgcccataa cgttgtcata
      961 accagcagcg atgaaattgc tcagggaatt attaccaggc tacaaaaaac acccggtgag
     1021 catatcgaaa ataatgggat catttacgat atactgccct tcctggagat gaatatttcg
     1081 atcatcacct gggcttcaac gaaaatgttg cagaaaggct ggcatcggca agtctttatt
     1141 tggttaccgc tcgggttggt gattggcctg ctggcagcga tgtttgtgct gcgtattttg
     1201 cgccgtatcc aatcaccgca tcatcggctg caggatgcta tcgaaaatcg tgatatttgc
     1261 gtgcactatc agccgattgt ctccttagcc aatggcaaaa ttgtcggtgc tgaggcactg
     1321 gcgcgctggc cgcagacaga cggtagttgg ttgtcaccag atagttttat tccgctggca
     1381 cagcaaacgg gcctttctga gccattgacg ctactgatta taagaagcgt ctttgaagat
     1441 atgggcgact gcttgcgtca gcatccacag cagcatattt cgatcaatct tgaatccacc
     1501 gtgctcacct cggaaaaaat cccgcaattg ctgcgtgaaa tgatcaatca ctatcaggtt
     1561 aatcccagac agatcgcgct tgaactcact gaacgcgagt ttgccgatcc gaaaaccagc
     1621 gccccgataa tttctcgcta ccgggaggcg ggccatgaaa tttatcttga tgattttggt
     1681 acggggtatt caagtttaag ttatttacag gatctggatg tcgacattct gaagatcgat
     1741 aaatctttcg ttgatgcgct ggaatataaa aatgtcacgc cgcatatcat cgaaatggca
     1801 aaaacactga aactgaaaat ggtagcggag ggaatcgaaa ccagtaaaca agaagagtgg
     1861 ctacgccagc atggcgtgca ctacggtcag ggctggctct acagcaaggc attaccgaaa
     1921 gaagatttct tacgctgggc cgagcaacat ttgtgaattt ttgggctgac ggcacgccgc
     1981 acagcccgtc acgctaccta ttcgtcgatg attgactcga atccgccata gatcattcgc
     2041 ttgccgtcaa acggcatgga ctcgccgaac tctttcatcc gtgggtccga catcatcttt
     2101 tgattagctg cgtcgcggac ctcttttgaa gggtattcaa tccagctaaa gacaacctct
     2161 tcattctctt ccgctttcac cgccatacga aaatcggtca ctttgccatc cggcacatcg
     2221 ctggcccagc attcgacaat acgaagcgcg ccaaactctt taaacaatgg cgcggcctta
     2281 gcggccattt cccgataggc atccttttta tcggcaggaa cggcaaccac aaaaccatca
     2341 acatacttca tcagacatac ctccgaagcg tactgcgcag ccagacaatt cttgcgacgt
     2401 cagttgtggt taaagtttag ccatgctggg attttttgac gaaacggtgg gaggcagaaa
     2461 tgggtggggg ccctgccagc tacatcccgg cacacgcgtc atctgccttg gctgcttcct
     2521 tccggacctg acctggtaaa cagagtagca ttgcgggaga accaacagag cccccattga
     2581 gagcgttgaa aaccaacgcg caggcgcatt atcactgctg ccccggctaa ttgcaaccca
     2641 ttgcgtgcca gatgctggtt tcttgcgcaa tgcgacttca ctcgggcgtt tttccagatt
     2701 atgctgaaaa attacctcag gaagaaaaga tggaaaaaga agattcattt ccccaacgcg
     2761 tctggcaaat cgtcgccgct attcccgaag gctatgtcac cacttacggt gatgtggcga
     2821 aactggcggg atcgccccgc gccgcgcgcc aggtgggcgg tgtgttaaag cgtctccctg
     2881 aaggcagcac cttaccctgg caccgggtgg ttaatcgcca tggcacaatt tcgctaaccg
     2941 ggccggattt acagcgtcag cgacaggcat tactggcaga aggtgtgatg gtatcgggaa
     3001 gcgggcaaat cgacttgcag cgttatcgct ggaactacta accctctccg g
//
LOCUS       DAJTSF010000228         2997 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000228 DAJTSF010000000
VERSION     DAJTSF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.235"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="OO335_004159"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="OO335_004159"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR6004301.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="OO335_004160"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="OO335_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6004302.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="OO335_004161"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="OO335_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR6004303.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2756..2938
                     /locus_tag="OO335_004162"
     CDS             2756..2938
                     /locus_tag="OO335_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HCR6004304.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQ"
BASE COUNT          679 a          817 c          816 g          685 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt ggcagtagac
     2941 tggccccctg aatctccaga caaccagtat cacttattta agtgatagtc ttaatac
//
LOCUS       DAJTSF010000229         2951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000229 DAJTSF010000000
VERSION     DAJTSF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.236"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="OO335_004163"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="OO335_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR6004305.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="OO335_004164"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="OO335_004164"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6004306.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="OO335_004165"
     CDS             2603..>2951
                     /locus_tag="OO335_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6004307.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAJTSF010000230         2894 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000230 DAJTSF010000000
VERSION     DAJTSF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.237"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..308)
                     /locus_tag="OO335_004166"
     CDS             complement(<1..308)
                     /locus_tag="OO335_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6004308.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     "
     gene            complement(355..1074)
                     /gene="yehT"
                     /locus_tag="OO335_004167"
     CDS             complement(355..1074)
                     /gene="yehT"
                     /locus_tag="OO335_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6004309.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="OO335_004168"
     CDS             complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="OO335_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6004310.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          748 a          774 c          657 g          715 t
ORIGIN      
        1 tcatcggttt tcagcgaaaa cgcgatgcgt aattttttca gcacgatgtt gttattaatg
       61 cgacgttccg gctccagtgc cggagcagac tcatccttgc cgcgtttttc ataaatcagg
      121 ccattgagga atgacgacaa aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct
      181 ttacgtagcc agacggcgat ctgttccgcg gtggcttcga cattatccag cgccagaata
      241 cgcaccaggt cattattatt ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta
      301 cttatcatga ggccttcagg tgttgatgag gcaaaaagcc attttagcag tcttttacag
      361 gccaatcgcc tcttttaagc ttttcagata acggcggctg accggcacgg ttaagccatt
      421 acgcaaaatc aactcggcct ggccgttatc ttccagacga atctcctgta aatgcgcgag
      481 gttaaccaga tactgacgat ggcagcgcag tagtggtgta cgactttcca aggtacgtaa
      541 tgtcaattcg gtaaagccct ctttcccttc gtggctggta acgtagacac cgctcatccg
      601 actgctgaca aatgccacat ctttcatttg cagcaaataa atccgactat gccccgtaca
      661 agggataaat ttcagcgcct gttgattttc cggtaacagc gaaacatcct gcttgctgcg
      721 ctcctgacgc aatcgcgcca gcgttttctc cagtcgcgct tcatcaattg gcttcagcag
      781 ataatcaaag gcatgttctt caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa
      841 aacaatatac gggcgatgtt ccgggtcaag catccccacc atttccagac cactgatgcg
      901 cggcatctgg atatcgagaa acagcacatc cgggcgcagt ttatgcaccg cgccgatccc
      961 ttccacggcg tttgaacact ctccaacgat ttcaatatcg ctctgctcct gcaaaaatat
     1021 acgcaggttc tcccgtgcta acggttcatc atcgacaatt aagactttaa tcatgcctcg
     1081 tccctccatg gtagtcgtaa cgttattcgg gtgtaactat caggctcaca ggcgacgctt
     1141 attccatagt catcgccaaa ccgttcacgt aaacgcttat ccaccagatt catccccagc
     1201 ccactggcat tggttaccgg ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa
     1261 tgttgcccct cacgtcgggc gctgattgcc actcgccctg tatccagcag ttgtgatgtc
     1321 ccatgtttaa tggcgttttc cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg
     1381 gataattctt gcggaatagc aatgttgacc tgcaaccgcg actggaagcg cgccttttca
     1441 atttgcagat aagcgttcac atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc
     1501 cgctttaagt ttttgcggaa aaaagtggaa agatactgca ccagctggct ggcctgttcg
     1561 ctgtcgcggc ggatcaccgc tttaatggtg ttaagcgcat taaacaaaaa atgaggattc
     1621 acctgggcgt gaagcagttt gatttctgac tgggtgagca tcgctttttg ccgctcatat
     1681 tgccctgcaa gaatctgcgc cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt
     1741 attgaactga ataaccggtt tttggcttca tacaatttga tggtgcccat cacgcgctga
     1801 ttttcgccac gcaacggaat taccagcgtc gaccccagtt tgcattgcgg atgcaaagag
     1861 caacgataag gtacttcgtt gccatcagcg tagaccactt caccggtttc aatcgctttt
     1921 aaggtgtaag tcgaagaaat cggtttgccg ggtaaatggt ggtcgtcacc aattccggta
     1981 aaggccagca atttctctcg atcggtaatc gcgactgcac caatatccag ctcctgatac
     2041 agtacctgag ccactttcat gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc
     2101 gtcgaggctg ccactttcag cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc
     2161 gcgcgtttat cgagcaatat acgcataaac agcgccgcgc cgacggtatt ggtgaccatc
     2221 attggcgcag caatattact caccagacgc accgcatctt cataaggtcg ggcgatcgca
     2281 aggatgatca gcatttgcac catttcagcg acgaacgtga cggcaccggc ggtaatgggg
     2341 ttaaagactt tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc
     2401 aatccttcaa cgatggttga gatcatgcaa cttagcgcgg tcatgccccc catcgaatat
     2461 cgatgtaagc cgccggtcag accaaccagc ccaccgacga ccggaccgcc gagtaagccg
     2521 cccattaccg cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg caacccaaac
     2581 caggtgccca tgatgcagaa gatggaaaag acgatgtagc agagaaattt atgcggcaga
     2641 cgaaccgtga cctgcattaa cggtatgaat aatggcgttt tactcattaa ccacgcaatg
     2701 actaaaaaaa cgcacatctg ctgaagcagc agcaacacca gattaaaatc gtacataccc
     2761 gcaaaccaca cttcccttta aaacgcgtaa catacattgc ctgcgtttaa ctttctttga
     2821 actcttgcag aaaaatgaga attcgtgagt acgatcactc aaaatcgcct ggcaaaaata
     2881 aaatcaccct atag
//
LOCUS       DAJTSF010000231         2888 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000231 DAJTSF010000000
VERSION     DAJTSF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.238"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..779
                     /gene="lsrF"
                     /locus_tag="OO335_004169"
     CDS             <1..779
                     /gene="lsrF"
                     /locus_tag="OO335_004169"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR6004311.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="OO335_004170"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="OO335_004170"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR6004312.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1133..1891
                     /gene="tam"
                     /locus_tag="OO335_004171"
     CDS             1133..1891
                     /gene="tam"
                     /locus_tag="OO335_004171"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR6004313.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1895..2809)
                     /locus_tag="OO335_004172"
     CDS             complement(1895..2809)
                     /locus_tag="OO335_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR6004314.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          760 a          667 c          771 g          690 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aaatgtctga
     1141 ctggaacccc tctttatatc tgcacttttc cgctgaacga tcgcgtccgg cggtggagct
     1201 gcttgccaga gtgccgctgg agaatgtcga atatgttgcc gatcttggct gtggcccagg
     1261 taacagcacc gccctgctgc atcaacgttg gcctgcggcc aggataacag gcatcgactc
     1321 gtctccggcg atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc
     1381 agacatccgc aactggcaac cggaacaggc actcgatctg atttttgcta atgcctcact
     1441 gcaatggctg cccgaccact acgaattgtt tcctcacttg gtttctttac ttaatccgca
     1501 gggcgtgctg gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg
     1561 cgaagttgct tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca
     1621 tgcttactac gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccactta
     1681 ctatcaccag atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg
     1741 tccgtggtta caggatctga ccgagagcga acagcagctt tttcttacgc gctaccatca
     1801 gatgctggaa gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg
     1861 tctgtttatt gttgcccgcc gtacggagta attatcacgt cagctggtaa tgacgatcgg
     1921 gaagaatttt tgctggaatt tctgcttcat cgttcatctg taacaggtca atttcaatag
     1981 cgttgcagat ggcatccagc ggtaaatcat tgttttcagt accgaacgga tcttccagtt
     2041 cttccgccag acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg
     2101 tcatgtaatg caggtccacg accagcgcga acggcagcat gatacaaaac agataaacgg
     2161 tacgatgcaa aatcagggtg taggcaaagg gaattggcgt gtaggcaatg cgctcacagc
     2221 ctgccagaac cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatatctg
     2281 aaagctgtcc attgcggcgc tgaaccgcca accattctcc cattattaac aagatacggt
     2341 tagctggaga gttcgaagcc agtacacgct gaagatctcc agtcttgaga taatgagcca
     2401 gcacttccgc ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt
     2461 gcagcctggc aaactccctt acacttgccg aatccggcaa tgtcgttttt acctctcgca
     2521 gtaacgaccg tgaggcaatc atcaactgcc cccaaagttt tcgcgcttca acgtaacggg
     2581 cgtacccggc attattacga aaaccaagaa aaatggcgat ggcgacaccg agaatgctga
     2641 acggtgcgag ggtgaatttg atgcccagat gcgtgtacca gggcagcatg aatataacag
     2701 caatagaaaa gagaaaattg agtagtaagc gcgaggatat cttggataat actgagccgt
     2761 gccagacaaa aatacggcgc agccagtgtt gttgtggacg aacaatcatg gttatcttca
     2821 ggcgtggaaa agtcgcccta ttaaacgtga ttacgatcac attctcaaga cgctacttac
     2881 aaattacc
//
LOCUS       DAJTSF010000232         2768 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000232 DAJTSF010000000
VERSION     DAJTSF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.239"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..488
                     /locus_tag="OO335_004173"
     CDS             <1..488
                     /locus_tag="OO335_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HCR6004315.1"
                     /translation="IDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWV
                     FVYKESCTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAG
                     VPLSVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTND
                     V"
     gene            837..2009
                     /locus_tag="OO335_004174"
     CDS             837..2009
                     /locus_tag="OO335_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6004316.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2063..2686)
                     /locus_tag="OO335_004175"
     CDS             complement(2063..2686)
                     /locus_tag="OO335_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6004317.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
BASE COUNT          856 a          573 c          537 g          802 t
ORIGIN      
        1 aaatagatat gcagcgccgg gtggcatgga taaacccgga agagagtaaa tcaaaccgcg
       61 caattggcgt tgcgctgaat gatactgcat gtcgcgtatt gaaaaaacaa atcgggaatc
      121 atcaccgttg ggtatttgtg tacaaggaaa gctgtaccaa accagacgga acgaaagcgc
      181 caacggtcag gaagatgcgg tatgacgcaa acacagcctg gaaagcggcg ctgagacgag
      241 caggtattga tgatttcaga tttcacgact tgagacacac ctgggcaagt tggctggttc
      301 aagccggagt cccgttgtca gtgttacagg aaatgggagg ctgggagtct atcgaaatgg
      361 ttcgtcgata tgctcacctt gcacctaatc accttaccga acacgcacgg caaatagact
      421 cgatcctgaa cccatcggtc ccaaattcgt cccagtcaaa aaataaggaa ggtactaatg
      481 atgtgtaact tattgattta aatggtgccg ataataggag tcgaacctac gaccttcgca
      541 ttatgaatct gtagcaccaa tcataactat ctgttttagc aagcattaac cgcattcact
      601 aagctatagt tgatgacaca aacagaaagt tgatgcatga tgttgccata tatatgtcac
      661 aaatacggca caacgatctt caaacatata gccacacatt cacagaagag cacaaagcct
      721 tgcaatccag tgcaaagctt ggtgtgtctc cgttttgttt catcagttac acataactat
      781 atgatcatta actaaaagtc agtatacttg gagaaatttt aattgcgtaa tagagcatgc
      841 atatgattga aattaacagt ttattattaa taacatccgt gatattgatg tcgttattag
      901 ctgtggggtt atttgataaa atttccccaa taaatcttgt tgagcacggc aggaataatc
      961 aaatagatgg aatgcggggg ttcttagcaa ttttcgtgct tattcatcac gcagcaattt
     1021 ggaatggata cttgtcatct ggagtatggg aagcaccttc atcaaatctg ttagcaaatt
     1081 taggccaagt tggtgtgtca ttctttttta tgattactgg ttatctgttc ttttcaaaga
     1141 ttatctctgg agatcaggac tggacaaggc tttatgtatc aagattacta cgattaaccc
     1201 caatgttcat agttagccta tgtctcattt ttatcattgt aggttttaag tctggatgga
     1261 gaatgcaggt atccacagaa gagctttttg tgtcaataat gaagtggttg ccattcactg
     1321 cgctaggtat gccgaacatt aatgacgtaa aagattcatt tactatcaat gccgctgtaa
     1381 catggacact tgtatatgaa tggttctttt atttttctct tccggtaatt tccgcgctca
     1441 taaaaagaaa agtcagtatt tacatggtta tgattagcgc aatatcgcta tttgttttca
     1501 ttttattttt cagtaaaata cacatagcat catttctttt tgggctcctg gcatttttac
     1561 taaataaatc aaagatagtc aatggaattg ccaaagcaaa agtaacacca attataataa
     1621 ctgcgataat ggtttttgaa atgacgtatt tcaaaacaac ttacgcaccg ctaccactga
     1681 ttctttgtgg aataacattt atcattatcg catcaggttg tgatttgtat ggaatattaa
     1741 gattaaatat aaccagaaag ctaggagaaa ctacctacag cgtttacctt ctgcatggga
     1801 tattccttta ttgcctaatg acgtggatta ttcctaataa ttacactgaa aatacattta
     1861 tcatactggt aagcacaact gcatttctta ttacgttcac atcatgccta acttttaaat
     1921 taatagagac gccattcatc aaattaacaa aacaaaccac aactttagta aaagaattaa
     1981 tacccacatt aacaaacaac aatcaataaa caataaacat aagccgccct acatatatgt
     2041 ccaaatgggg cggcgaatgt atttatatta gtctggtaat gctaaatgca aacgaactgg
     2101 agattcctgc cccgctaagc gtattgcgca gggtcaccct aaatgtatct acgccaatat
     2161 aagtaacatc acaactaact gaatcaggcg cagatatagg tgttatctgc acaatataac
     2221 cgctagcaac tgctgcgagc ggatgagaaa agctaacatc atacacacct gtagaaacca
     2281 cgttaaccga tgaaacacca gtagcaacgt cgatactacc attggcgtta atttttcccg
     2341 agcgtgtgac tgacaatgca gaagccgatg ttaccttact ggttgtttct gtaaaaacac
     2401 acccaatgtc aactacctcg caattagctc ctgttatcca gtatggtcgt tctgcacttg
     2461 gctgataatc ccctgcgctc tggaacgttg tgccgttgag tatgactgta acttttcccc
     2521 cacctgagct tgttacttga atattcgtgt gcgtgtatct tgcactgctc acgcgatgaa
     2581 aatttccacc gtttataacc acagttacag ggcgcgtccc agtattatct atagcaaggt
     2641 ctgccccccc ggcattagcc tcaaagtatg ggttgtttaa taccaaagcg cacgaaccgt
     2701 taaggccatt tatagctaat tgcgcaccac ctgtactgga atccccctga gtaccatttc
     2761 cttcacaa
//
LOCUS       DAJTSF010000233         2749 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000233 DAJTSF010000000
VERSION     DAJTSF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.240"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="OO335_004176"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="OO335_004176"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6004318.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..>2749
                     /locus_tag="OO335_004177"
     CDS             1647..>2749
                     /locus_tag="OO335_004177"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6004319.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICT"
BASE COUNT          708 a          623 c          722 g          696 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg
     1981 cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc
     2041 tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa
     2101 cggataaacc aaacctagtg tgcggtccgg tacaaatctg ctggcataaa ttcgcccgct
     2161 actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt atggacccga
     2221 aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga
     2281 cctacaccgg taactatgag ttcccacaac cgctgcacga tgcgctggat aaattccagg
     2341 ccgacaccgg tatcgacatc gacatgcaca tcgacgctgc cagcggtggc ttcctggcac
     2401 cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg
     2461 cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg
     2521 aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctgggtggt caaattggta
     2581 cttttgccat caacttctcc cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc
     2641 tgcgcctcgg tcgtgaaggc tataccaaag tacagaacgc ctcttaccag gttgccgctt
     2701 atctggcgga tgaaatcgcc aaacaggggc cgtatgagtt catctgtac
//
LOCUS       DAJTSF010000234         2734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000234 DAJTSF010000000
VERSION     DAJTSF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.241"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            88..153
                     /locus_tag="OO335_004178"
                     /pseudo
     CDS             88..153
                     /locus_tag="OO335_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="OO335_004179"
     CDS             381..629
                     /locus_tag="OO335_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6004320.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(745..1014)
                     /locus_tag="OO335_004180"
     CDS             complement(745..1014)
                     /locus_tag="OO335_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6004321.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            1071..1739
                     /locus_tag="OO335_004181"
     CDS             1071..1739
                     /locus_tag="OO335_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6004322.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            2055..>2734
                     /locus_tag="OO335_004182"
     CDS             2055..>2734
                     /locus_tag="OO335_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6004323.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          835 a          589 c          538 g          772 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat
      661 cattcaatac tcgcactatc gaaagtttac cagccaaccg cagcacgtcc tgcatacgtc
      721 gtgtctgcgg tttttctttt tcgcttacat tgtgtctggt tcttccggcc actcaatatc
      781 aggtgcagtt gatgtatcaa cacggttcag caacacccga tacttcttcc aggcttccag
      841 caacgagttt tcttcctccg ttgcgatctc cagatctaca gcatcctgaa gtggcgcaat
      901 atgctcactg aatccctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc
      961 ctgctgtatc gaagcatacc aggctatttc aatatcgcta tgctgcggca gcatttaacc
     1021 ccttgtaatt catcgccata attgatttaa ttcacaaata aaactataat atggtgaaat
     1081 taatgaaaaa aaacacagat gatggggcta aaatttacac accacttacc ctaaagcttt
     1141 atgactggtg ggttttggga gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac
     1201 accttcttcc acactttctg gaacatttag gtaacaacca tctggatatt ggtgttggaa
     1261 ctgggtttta ccttactcac gtacctgaga gtagtctgat atctttaatg gatttgaacg
     1321 aagctagcct gaacgcggca tctacaaggg ctggggaatc aaaaattaaa cataaaatta
     1381 gccatgatgt ttttgaacct tatcctgcgg cgttacatgg tcaatttgat tccatttcca
     1441 tgttttacct tcttcactgc ctgcctggaa atatatctac aaaaagctgt gtaatacgca
     1501 atgcggcgca ggccttaact gatgatggaa ctctatacgg agccacaatt cttggcgatg
     1561 gagttgtgca caatagcttc ggtcaaaaac tgatgcgcat ttacaatcag aaaggcatct
     1621 tttcaaacac aaaagattcc gaagaaggct taacacatat actctcagag catttcgaga
     1681 atgttaaaac caaggttcaa ggtactgtag taatgttttc cgcttcaggg aaaaaatagc
     1741 atccaaccgc agcacgttct tgcttaagac gtgctacggt ttcctttacg atttggggtt
     1801 gcgactttac ctctattgat aatgcattcc ggcagaacgt tcaaatatga acgtacgata
     1861 tttaactaac cgaaaaacaa aatatagcac aggcgagaca tttaccatct aagaaaatag
     1921 tatcgttttt ttacagttaa atcaacatca cttccttaaa atgaaaacaa taaatttcaa
     1981 tcctgaaaca ttttatttca gcttattatt ccccagtatc acattttatc aggatatcct
     2041 tctgcgaggt tattatgctt cctgtaaata atccccccct atccactgga aacgtctctt
     2101 tttacagaac tacatcaatc gacaatgttc acaataatta tctctccgaa tgggttgaat
     2161 ggactaaaaa cagcatttcc ggagaaaaca gggaaactgc ttttacccgg ctccaattat
     2221 gtctggagaa cagtgaaaca tcgttggact tatcttgttt aggtctcaga tctctaccac
     2281 gattgcctga caatcttgat gaaattaatg taagcaataa ccaactatca atgctccccg
     2341 agctaccaag ggcattgaaa gagctgaatg caagcagtaa tcaattatct gcacttcctg
     2401 aattaccagt gtcgctggaa tatataaatg tgagtgataa ccatttgttc gcacttcctg
     2461 aattacctgc gtcactagaa tatattaatg taagtgacaa tcacctgtct gtacttccga
     2521 ggttaccaat gtcattggaa ttacttgatg cagccagaaa tgctttggaa gtaataccag
     2581 attttccaga aagagatgat catattataa gaatattctg gcttaatcag aaccggatca
     2641 cggcaattcc ggaaagcata cttggcctca gttctgatag cgttgtcaat cttagagaaa
     2701 atcaactatc tcccagaata atgcaaactt tgtt
//
LOCUS       DAJTSF010000235         2714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000235 DAJTSF010000000
VERSION     DAJTSF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.242"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            6..224
                     /locus_tag="OO335_004183"
     CDS             6..224
                     /locus_tag="OO335_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6004324.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="OO335_004184"
     CDS             226..591
                     /locus_tag="OO335_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6004325.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="OO335_004185"
     CDS             588..1253
                     /locus_tag="OO335_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004326.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="OO335_004186"
     CDS             1253..1618
                     /locus_tag="OO335_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6004327.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2073..2228
                     /gene="hokD"
                     /locus_tag="OO335_004187"
     CDS             2073..2228
                     /gene="hokD"
                     /locus_tag="OO335_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6004328.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          771 a          557 c          696 g          690 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg
     2101 ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg aaagacctct
     2161 gcgaggtacg aatccgaacc ggccagacgg aggtcgctgt cttcacagct tacgaacctg
     2221 aggagtaaga gaccaggcgg gggagaaatc cctcgccacc tctcatgtgt caggcatcct
     2281 caacgcaccc gcactaaacc cgcttcggcg ggttttttgt tgcgtgctga atacgcaggg
     2341 tgaaaaataa ccatatattt gattatatac acaacaaaaa ataaaagtca ttgtacttgc
     2401 acattaaaca atcaaatata cggcgtgaaa taaatattta tcagattaat atttttgtct
     2461 ctatgtggat ataaccgttt gtacttataa acctgggggc atcgtggaaa aaataaagaa
     2521 actatttagt agcaaatacg cagtcatacg tcgtgatgac ctgtcagtta tagtcgaaat
     2581 ggattacttc cctgaaaccc caaaatcaat aatgtatcgt aatggtcgaa aggcaatttt
     2641 tttaccgatg agggtaagtg acattatggg aaatgataaa ctgctggatg aattgcgagt
     2701 cagagcatcc tgtt
//
LOCUS       DAJTSF010000236         2711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000236 DAJTSF010000000
VERSION     DAJTSF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.243"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            50..2572
                     /locus_tag="OO335_004188"
     CDS             50..2572
                     /locus_tag="OO335_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6004329.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2583..>2711
                     /locus_tag="OO335_004189"
     CDS             2583..>2711
                     /locus_tag="OO335_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6004330.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          726 a          585 c          677 g          723 t
ORIGIN      
        1 tgacccgata aaaatgacgc tgtaagcaag ggataaggat aggcgtatta tgatgacgac
       61 cagaatagtg gttggcctca cggcagggac gtgtctgatt ttctcgcaaa acctgatggc
      121 cgaggtcagt gtattcaatc cggcgcttct ggaaatcaac catcaatccg gagtcgatat
      181 tcgccagttt aatcgggcaa acctgatgcc cccaggtgtt tatagcgttg atatttttat
      241 caacggtaaa atgtttgaac gtcaggatgt gacatttgtt caggataatc cagatgctga
      301 tctgcacgct tgctttattg ccattaaaaa aacactgtcc tcctttggca taaaagttga
      361 tgcgctcaaa tcgttcaatg atgtggatga gacggtttgc ctcgatcctg ctccacgtat
      421 tgaaggctca tcctggcagt ttgacagtga taaattgcag ctgaatatat ccattcacca
      481 aatctacatg gacgcgatgg cttatgatta catcagcccc acgcgttggg atgaggggat
      541 taatgcgctc accatcaact acgatttttc tggttcacat acactacgtt cagattatgg
      601 ttcacaagag acagatacca gttatctcaa tctgcgcaat ggactgaata ttggaccgtg
      661 gcggctacgt aattacagta ctttaaacac cagcgatggc cgtgcggaat acaactccat
      721 tagtacctgg atacagcgcg atattgccgc gttaagaagc cagattatga ttggtgatac
      781 gtggacggcg agcgatattt tcgacagtac gcaaattcgc ggcgcgcgtt tgtatactga
      841 taacgatatg ctacccgcca gccagaatgg ctttgctcct gtggttcgtg ggattgcaaa
      901 gtccaacgcc accgtcatca ttcggcagaa tggctacgtg atttatcagt cagccgttcc
      961 acaaggtgct tttgagatca ccgatctcaa caccgcaagt acaggtggcg atttggacgt
     1021 aaccatcaaa gaagaagacg gtagcgaaca acgattcacc caaccttatg cttcattggc
     1081 gattcttaaa cgtgaaggtc tgacagatgt tgatgtcagc gtgggtgaat tgcgcgatga
     1141 agacggattt acaccggacg tccttcaggc gcaaatactt catggttttt cccacgggat
     1201 cactttatat ggaggtatgc aggctgctga aaattatggt tctgcagctc tgggtgtcgg
     1261 taaagatctt ggcgctttgg gcgcaatttc tttcgatgtg acacatgctc gtgcgaattt
     1321 tagccatgat gatacagaaa cgggtcagtc atatcgcttt ctctattcaa aactatttga
     1381 cgacacagac actagcttgc gcctggttgg ctatcgttac tccaccgagg gctactatac
     1441 cctcaatgag tgggcatcgc ggcgcaacag ccctgaagac ttttgggaaa caggtaaccg
     1501 acgtagtcgc gtggagggaa cgctaacgca gtcgttgggg agagattatg gcaatttata
     1561 cctgacatta agccggcaac aatactggca taccgatgat gtcgaacgat taatgcaatt
     1621 tggctacagc agtagctgga agcgtctctc gtggaacgtc tcctggagtt attccaatac
     1681 tgccagacag gggacgggga acaaccatgc cagtgataac accagtgagc agatctacat
     1741 gctctcttta tctgttcctt tatcgggctg gtggggtaat agttacgcca cctattctgt
     1801 ttcgcaaaac gataattccg gtagctcaca tcaactcgga ctcagcggta cggcgctgga
     1861 aagaaataac ctttcatgga atttaatgca gtcctataac agtcatgatg atgaggttgg
     1921 cggtaatatg tccctgacct atgatggctc ttatggcacg gtgaacggca gctataacta
     1981 cagccaaaat tcccagaggc tgaattatgg tatcagaggg ggaattctgg cacacagcga
     2041 aggggtaacg ttaagtcagg agttaggtga aactattgct cttgttaaag cacctggggc
     2101 cgccaggtta gaaatagata atatgcgcgg tgctgcgacg gactggcgcg gctatacggt
     2161 caagacacag ctaaaccctt atgatgaaaa tcgggtagca atcagcgata actatttctc
     2221 gaagtcgaat atagaacttg ataataccgt cgttacgatg gttcccacgc gtggtgcagt
     2281 ggttaaagcg gagtttgtga ctcatgtggg ttatcgcgtt ctcttcagag tgttaaatgc
     2341 aaatggtaaa ccggtacctt ttggagccat tgctgcgata caagatgcaa gtttggcaga
     2401 ttcaggaatt gtcggtgacc gtggcgaact ttatctttct ggtctaccag aaaaaggaca
     2461 ggttacgtta tcctggggag aaaacgcctc aacaaaatgc atcttcaatt attcattttc
     2521 gacaccagaa agtgagagcg gattaattga acagggtgtg acatgtcatt aaaaggattt
     2581 ttatgaacaa gtatatcaaa cagtggtgct ttgctgtgtt tatgctctcg ttaagtagtg
     2641 tagcacttgc agctcctaaa ggtatctgta cccctgataa tggcgtattt cacagtacgc
     2701 ttgatttctc g
//
LOCUS       DAJTSF010000237         2670 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000237 DAJTSF010000000
VERSION     DAJTSF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.244"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(42..587)
                     /locus_tag="OO335_004190"
                     /pseudo
     CDS             complement(42..587)
                     /locus_tag="OO335_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1249..2238)
                     /locus_tag="OO335_004191"
     CDS             complement(1249..2238)
                     /locus_tag="OO335_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004331.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2322..>2670)
                     /locus_tag="OO335_004192"
     CDS             complement(2322..>2670)
                     /locus_tag="OO335_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004332.1"
                     /translation="TSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAER
                     IAKELASQKERTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIV
                     ADLESGGNWMDSL"
BASE COUNT          690 a          624 c          644 g          712 t
ORIGIN      
        1 cctcgcttag gcctgtgtcc atattacgtg ggtaggatca gaattccatc cagtcgtttt
       61 gggttgctaa tgatgtcctg catccgctta atctggtcgg gcgtagacat ataaccccac
      121 agggtgagca gaaccttttt tgcggctaca ctatttcctt ggtctgatac taatgccata
      181 tagccccata aactatccct cagccagaca gcatcatagt gggtttctgc cgctgttttg
      241 tcatcttcga aagtagaagc aagaataaac gttccccagg gtttctccgt caacgttaac
      301 tttcgtgtgt tttctgttaa tgttagcaat ccttgagttt cgtctttggt atagtgactc
      361 tttatttcat tttttaattt ggctgagtga atcagaagac gataattagg atacttatct
      421 ggatttaacg ataaataact tttatctgtt gctgcccccc tggaattaag aatgttaaat
      481 acagattcgc ttgtcacttc tgcctctgtc gcctggcatg tgccgcacga aaatatacca
      541 gcgagcagaa gagttaatgc agatttccct ttcataaaat aatccatcaa tcgtttgtta
      601 aaagcgtaga taaccactta ttcaacaact tgaaaataag gtattttacc tgagttgtta
      661 caaaaggatt gcattgcgta aacgcttttt atttacaaaa aaatggggaa gtgttacagc
      721 gattgcgaaa agcctgaagt gattgaatta agataatata gaaaatcaga tacagcaaaa
      781 atggcagatt gatcaggtga agataaattg gagcgggcga agggagccta cgacaaatcg
      841 aagacaacca gctaacctat tgtttcttaa agacaggtga atccagttgg ttgtagtgat
      901 agtcacacat ttagagatag aaggtcaaga cttgaagcac gtaatattcc ctctaaagga
      961 atagaactca cggtctgact acccggccct tttggtctgg ctcaggtcag gctatggggg
     1021 cttttggctc gctctttata ttagatgagg tctcacattt ttcagtcaaa actcatcgtc
     1081 taatagccca tagccagacc accagtccca gctttagcag gaagaccacg agatagactt
     1141 tgtgtaggtc catttggccc tcctactttc tgtattcaca aactagatta ctagtacaaa
     1201 ataaaggcca gacatccagt tcggaagtct gaccaatagt cgttattgtt agttagcttc
     1261 tttagtgaag ctcaggcgtt tgactacagg caggacagca gtagccgagc cagtacccat
     1321 cttgatggtc gtagcagctt cagctacaac aaacaccttg ccgagaatct caaggtcgtg
     1381 agactgggag tctccatcac gtgaaaggac cacagtaatg ctctcgccat cgtgacggaa
     1441 gcaaccgaag ctcagagccg taaaacgctt agccatcatg aacacgccac ggtcttcacc
     1501 gttgtaatag gcgacgtcag tccccagcat atcgacgata gcgtcttcac caccgaagcc
     1561 ctgcttagca gccagacggg agagagcctt gaggtgtgac tcatgggcga taatgccgta
     1621 gtcagaaacc tcagaaccga aagaagactc cgtgttctcg tacagggcat ccatgatagc
     1681 gtcagcctta gcggtcaggc taccagtcag ggccggaagt tctacggtct tcagcagctt
     1741 tgaggactcc agcacgtcaa ccatcttaga gttaatggta cgaatcacgt tatcgcagat
     1801 gttcagcgcg tcctgctgga gcgtcccctg agtgaaacca gtggtcgact tctggtccca
     1861 aagctttgtt tggtgagaca cgaggatcgc ctgttcaatc ggctcaacgg tgatttcagt
     1921 gccagacgga gcggtcacaa cttcaggtcg cttaccacca cggacaggga ccagcacaga
     1981 gcggtcagcc agagtgtgag caatgattgg agtcggtaag ctgttacggg tcggcaggac
     2041 ttctacaggg aacgggatgt tatcttgagc gtctttaagt acaccgttca gcatagcctt
     2101 aacagtctta gctaactgga gggcatagac ctgagggtcg gcgttagtcg gcagaccttc
     2161 attgacctga atgttgtcgg tatcggtgat gcgtactggc tcaatgccaa actggaaagc
     2221 gtttttagtc atattcatta taattaatct ccttgagtca aatacttgaa gtccctaaca
     2281 ggcagtcagg gaccgtgtgt tctcagatga gggacgcttg cttacaagct gtccatccag
     2341 ttaccgcctg actcaaggtc agcgacgata gcggcacgtg agcgaccagc ggaatgagta
     2401 gcgctgcgga ggttttcacc ataggtccgg cgttcagctt tgacctgttc gcggcgctgt
     2461 agctcttttt gccattcgtc ttcggtcatg aacatttcgc gggtacgctc tttctgagac
     2521 gctagctctt tagcgatacg ctcagcttca gctcgtttgt tcatctggcg gcgttggtag
     2581 gcggtcatca ggtcattact gaagaagtta ccaatggtca cgccagcttc agccagagct
     2641 tccagtaact ccatcgcctc gcggctggtg
//
LOCUS       DAJTSF010000238         2572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000238 DAJTSF010000000
VERSION     DAJTSF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.246"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(64..822)
                     /locus_tag="OO335_004193"
     CDS             complement(64..822)
                     /locus_tag="OO335_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR6004333.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO335_004194"
     CDS             complement(830..1933)
                     /locus_tag="OO335_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6004334.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2572)
                     /locus_tag="OO335_004195"
     CDS             complement(1943..>2572)
                     /locus_tag="OO335_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6004335.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          711 a          683 c          601 g          577 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aa
//
LOCUS       DAJTSF010000239         2542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000239 DAJTSF010000000
VERSION     DAJTSF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.247"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..197)
                     /locus_tag="OO335_004196"
     CDS             complement(<1..197)
                     /locus_tag="OO335_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCR6004336.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFS"
     gene            390..893
                     /locus_tag="OO335_004197"
     CDS             390..893
                     /locus_tag="OO335_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6004337.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(939..1355)
                     /gene="rraB"
                     /locus_tag="OO335_004198"
     CDS             complement(939..1355)
                     /gene="rraB"
                     /locus_tag="OO335_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6004338.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1517..2530
                     /gene="argF"
                     /locus_tag="OO335_004199"
     CDS             1517..2530
                     /gene="argF"
                     /locus_tag="OO335_004199"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR6004339.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          701 a          592 c          591 g          658 t
ORIGIN      
        1 gaaaatcgtt gtccgttaac ttccatatta gcataaagat aacgcttaca tttcattaat
       61 gcccatggta aatagatacc tagcgttata atcgttaaca acacattcac cacacaaata
      121 agaaaatatc tctcacctgt accatgaaaa acaaaagaat gggacggaac atccatttca
      181 ttaataactt gagccataaa aatcccctga attaaaaaat ccattgatat aatttataga
      241 tacagacagt aaaatgttct gccacgttac aatactctta tcagtaatag gctggcaaaa
      301 actttattgc tttataagac gcttgcgcac gtaagaagag agtggtatta aacaaatgta
      361 taattacttt atttttattg ggttaaacta tgaataacat tgcgccgcaa tcacctgtaa
      421 tgcgtcgcct gacgctgcag gataatcctg ctatcgccag cgtcatccgt caggtttacg
      481 cagagtatgg tcttaccgcc gataaaggct acaccgttgc cgatccgaat ctcgacgaac
      541 tgtatcaagt gtacagccag cctggccatg cttattgggt ggttgaatac aatggcgaag
      601 tggtcggcgg cggcgggatt gcgccattaa caggtagtga agcggacatt tgcgaactgc
      661 agaaaatgta ctttcttccc gctattcgcg gtaaagggct agcgaaaaaa ctggccttaa
      721 tggcgatgga gcaggcacga gagatgggct tcaaacgctg ctatctggaa acgaccgctt
      781 ttttaaagga agccattgcg ctttatgagc atttgggctt tgagcatatc gactatgcgc
      841 ttggctgcac gggccatgtc gattgtgaag tgcggatgct acgcgaactc taaaaaaatg
      901 cccgctagta tgtgctatcg ggcattttaa tcaattaatt agtggcgaac cccgtcatcg
      961 tcttcatcga caaaatcttc atcgtcgcca tcttcgccgt taggatcttc aaagtaagtg
     1021 ccccaaccgt cgtactcaac gtcaaatttc tccgccagcg tcatcagttg ttcaacctgg
     1081 gcatcgatca gatcggcatt caacgcgcac tcgctgagga tgtcgcagca aatcacgata
     1141 tcaccatctt caacttccag ctcttctgga tcggtcactt cgtaaccgag tttaaacgct
     1201 tcaactgccg ctttttccag agtttccaga tcgtctgcgg aaagatggtg ttcgatggtg
     1261 tacagcgcgt ccggatcgct gccatcttcc agtaattctt caataatcaa acgtgtttct
     1321 tcacgctgtt cttccagttg ttccgggttt gccatggctc attcctctta atgtcctgcc
     1381 gatactctta ttgtcacaca ccactgacat tgcctccacc tttgtgacaa agatttatgc
     1441 tttagacttg caaatgaata ataatacaca taaattgagt tttaattcat tgaggcgtta
     1501 gccacaggag ggatctatgt ccgggtttta tcataagcat ttcctgaaat tactcgattt
     1561 cacgccagct gaactcaaca gcctgctgca gttagccgcg aagctgaaag ctgataagaa
     1621 aagcggtaaa gaagaagcca aactcactgg taaaaacatc gcgctcatct tcgaaaaaga
     1681 ctcgactcgt acccgatgct ctttcgaagt tgccgcatat gaccaggggg ctcgcgttac
     1741 ttatcttggc ccaagcggta gtcagattgg tcataaagag tcgattaaag acactgcccg
     1801 cgtgcttggt cgcatgtatg acggtattca gtatcgcggc tatggtcagg agattgtcga
     1861 aacactggcg gaatacgctg gcgtgccggt atggaacggc ctgaccaatg agtttcaccc
     1921 cacgcagttg ctggcggatc tcctcaccat gcaggagcat ttacccggta aagcattcaa
     1981 cgaaatgacg ctggtctatg caggtgacgc acgtaacaac atgggcaatt cgatgctcga
     2041 agctgcggcg cttaccggtc tggatttgcg tctggtcgcg ccacaggcat gctggccaga
     2101 agctgcgctg gttacggaat gccgcgccct ggcacaacaa aatggcggga atattacgct
     2161 gacggaagat gtagctaaag gagttgaagg tgctgacttt atctataccg atgtatgggt
     2221 gtcgatgggt gaagcaaaag aaaaatgggc tgaacgcatt gcattactgc gtgattatca
     2281 ggtgaacagc aagatgatgc agttgaccgg taacccggag gtcaaattcc tccattgcct
     2341 gcccgcgttt catgacgacc aaacgacgct tggcaaaaaa atggcggaag agtttggcct
     2401 acatggcgga atggaagtga ctgatgaggt cttcgaatct gccgccagca ttgtatttga
     2461 tcaggcggaa aaccgcatgc ataccatcaa agcggtgatg gtcgcgacgc tcagtaaatt
     2521 aaataattaa tatctctata aa
//
LOCUS       DAJTSF010000240         2496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000240 DAJTSF010000000
VERSION     DAJTSF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.248"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..565
                     /locus_tag="OO335_004200"
     CDS             <1..565
                     /locus_tag="OO335_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6004340.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="OO335_004201"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="OO335_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR6004341.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="OO335_004202"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="OO335_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6004342.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..2403
                     /locus_tag="OO335_004203"
     CDS             2167..2403
                     /locus_tag="OO335_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6004343.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          643 a          549 c          645 g          659 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgt ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gaagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctagcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta
     2341 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
     2401 tgacaaaaca gggaagaaac aagaaaggaa gtaaagataa ttggtgcgag gggggggact
     2461 tgaaccccca cgtccgtaag gacactaaca cctgaa
//
LOCUS       DAJTSF010000241         2487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000241 DAJTSF010000000
VERSION     DAJTSF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.249"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>2487
                     /locus_tag="OO335_004204"
     CDS             <1..>2487
                     /locus_tag="OO335_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6004344.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQE"
BASE COUNT          728 a          577 c          602 g          580 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggag
//
LOCUS       DAJTSF010000242         2479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000242 DAJTSF010000000
VERSION     DAJTSF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.250"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..254)
                     /locus_tag="OO335_004205"
                     /pseudo
     CDS             complement(1..254)
                     /locus_tag="OO335_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(302..667)
                     /locus_tag="OO335_004206"
     CDS             complement(302..667)
                     /locus_tag="OO335_004206"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004345.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATIPAGINADG
                     MYCPECGTVHWPDGVIPPF"
     gene            complement(1420..1893)
                     /locus_tag="OO335_004207"
                     /pseudo
     CDS             complement(1420..1893)
                     /locus_tag="OO335_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1821..2187)
                     /locus_tag="OO335_004208"
                     /pseudo
     CDS             complement(1821..2187)
                     /locus_tag="OO335_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2189..2479)
                     /locus_tag="OO335_004209"
                     /pseudo
     CDS             complement(2189..2479)
                     /locus_tag="OO335_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          564 a          712 c          578 g          625 t
ORIGIN      
        1 ttctcactgt ctccgttatg gcgtacacca catcttccag taatcgccgc ttcaccggac
       61 tcaggacccc gtatcaccat ccggaacccc ttatccgcca catgccagga gaacctttcc
      121 ataaactcgt cgccactcat cacaaggttt tcttctcgtt ttgtccggtg gctgttgtaa
      181 cgcaggccaa tttcatcctg accggcataa tgttccaggc ggctcatctg caccggctgc
      241 ttctccagcc agttaagcca tcggtttccc tgctgaaaaa tatcggcaaa acggggaagc
      301 atcagaaagg cggaataacg ccatccggcc agtgaaccgt accgcattca gggcaataca
      361 tgccgtcggc gttgataccg gcaggaattg tcgcaaattc ccgctccgtg cagcgggcaa
      421 tttccggata cccttcgtca tcaacacgta caaaccagaa gaccagtttt ttgtttccgg
      481 catccacaaa gaacggaata ttcaggtctg cgcaacactc aacggcatcg tcaaaattat
      541 caaagcgcag aacctcagct tcttcttcgt caaaaaaatc atcttcgtga agttttacga
      601 catagcggag gagtttgctt ctttgaggtg caggttttcg tttgcgtggt ttagctgaac
      661 gggccatata actacctgaa agacaatgat attgtctgtt tttataacgg taatcacagg
      721 ccatgacaag ccgcagccgt caggccgcct actcggggcg gtttagaacg cttatttagt
      781 accagatctt acttataata aaggggcttg gcagtttgga taaactattg tccttattcc
      841 actaatacaa actttttgta aagtatatta aatatacttc atttcatact gaaatccccg
      901 gaagtattca atcaaacaat tacaccataa aaggaagagg gggccccaat taagttgtca
      961 agcatgttta ctgcgcaatc gcttggtgct ccgtccctgt accccgcatt ggtactctgg
     1021 cgctcatggg gcttccactt gcgcttctcc cttcacatcg gggtgacagg tccacgttcc
     1081 ccacaagagc ctgaatcgac ttcacgccgc ttttatgccg gatgccgcgc aggcagtaaa
     1141 catatcccac aggaacgact ccaccattgt tttgacatca tctatatgct ttcgacacat
     1201 catcagcggt tcactttcgt tcgtctcttc gaaccatacc tgatcggctc acgtccgacc
     1261 ttttcccatc tcgctcacca ccacatctct taaatgcagc agcagtggat ggtttggtgc
     1321 ctgcgcctgc acgccgacac caaggggcct tccctcatct cttgtgctgc atggctgcac
     1381 agggactcac gtccccagga cgcgccttcg tggcacacag tcatcggcat agcaacagta
     1441 agcaacggca ggtttccatt gccagttttc tttgacagca gtacttcggc ctcgctggat
     1501 actgtgattc cagtaccagc gatcctttct ggcttttcca ctcaggtagc gtttattcag
     1561 atactggtcg aactcattca gtatgatgtt cgacagcaac ggcgagatca caccgccctg
     1621 tggcatactt tcactgaccg cccggaagag accaacatca acacggcgtt ttccacagca
     1681 gtctcataaa acgtgtatca ctgatcctgc ggcagacggc tttcatcagc agccggtgat
     1741 gtactgtgtc gaagtagctg gacaggtcac cttcaataac ccggcgcccc gtgtttcccc
     1801 gcagtcggtg agctgtaata gtacgtgatg tctcccgccc acttctggtt cgggccactg
     1861 gcgtaaaaat cctgcgtcag cagattttct gatacaggca ggccgtgtgc acggtaactg
     1921 atcgggctga acttctggga ggcttttgcg ctcagttcct gacggcgcag actggctgcc
     1981 acggttttta cgttaaagtg gtaaccagag cacacagttc atccgtcagg cgtggggcac
     2041 cgtaacgctg gactgggtaa aagccttgcg gacaacgctg tcgcagtgct gacggaactg
     2101 cggacgtgtg tttatccttg tccgccgctg acaccacgta taccagccgc tgcgggccac
     2161 ccggagtacg cggcacattg ccttgatgac ggtttacgcg gtcgcctgcg gcaacggctt
     2221 tccggctgac ggtagtgtgg acctttttac cccggagaac accggcaagc cccataaccg
     2281 ccatgagacg tgccacagtg catctggcca ctctgatacc ttcccgtaac aactgatgcc
     2341 agactttacg cacagcgtat acctgatgat tctcatcgta tacgcgcagt atctctttct
     2401 tcagccagtc atcgcgctgc gcacgggcac tgcgtttatc atgatgatgt cgctgttgct
     2461 gacagtggta atacgttga
//
LOCUS       DAJTSF010000243         2419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000243 DAJTSF010000000
VERSION     DAJTSF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.251"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..243
                     /locus_tag="OO335_004210"
     CDS             <1..243
                     /locus_tag="OO335_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6004346.1"
                     /translation="AENDALRRKLDNGGRVLVKGKCPVPSSAETSSASGMGNDATVEL
                     SPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(332..844)
                     /locus_tag="OO335_004211"
     CDS             complement(332..844)
                     /locus_tag="OO335_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR6004347.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1039..1803
                     /locus_tag="OO335_004212"
     CDS             1039..1803
                     /locus_tag="OO335_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004348.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1754..>2419
                     /locus_tag="OO335_004213"
     CDS             1754..>2419
                     /locus_tag="OO335_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR6004349.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          682 a          539 c          614 g          584 t
ORIGIN      
        1 gctgaaaatg atgctcttcg gcgcaagctt gataatggtg gtcgggtgct cgtcaaagga
       61 aaatgccctg tgccatcctc agccgaaacc tccagcgcct ccggcatggg caatgatgcc
      121 accgtcgaac tctctccagt tgctggacga aacgttctcg gtatccggga cggaatcatc
      181 agcgaccaga cagcattgag aatgcttcag gagtacatca ggatccaatg ccttgggggg
      241 tagcggtaat tttactcatt atccttcaaa tcagattctg ttgtcagagg aatgggggaa
      301 gctggattcg caagagcttt ttatgggcag tttagctaaa aaaaggcaac acgaagctaa
      361 aactaccaac ataaagtgct gaccagctag cagctgaaat cagactcact gtatacactt
      421 tcactggatg aagttgcaac attcctgcca ccaaaggagc aatgtaacgc agcacggcga
      481 taaagcgcga agtgaaaagt attgacacag agttattttg caactgaagg cgaacccgtt
      541 ctattgtatt ggaacggctc gatattatac atgcaagttg agggatatgc ccaattatta
      601 taccaagatg gtaattaacg atagtaccgc accatgcgcc actcatgacc gtgattccag
      661 cttcccatgg tgaaagagtc gtcttgctga ctgtaattac agtggccatc ataattgatg
      721 ctggtggcaa tatcgccgaa atgagtaaag tggattttgt gaacgctatt ataaaaagaa
      781 gcccccatag gcgatatgga tgaagtacaa aatagttcat caaagcattt atccactcca
      841 tcaatgtctt tccttctgtg aatttttctt tgctatttaa ccgtgatacg acttaattta
      901 ggcttaaaca ccttacattt acaaggtact cctgtggggg gccctgccac ggagcgtcgc
      961 aggagtgagt gatgaggaag aattaaagca acctattgtg acaggcagcg ataacagtaa
     1021 atcacaggaa gcataatcat ggcaaaaccg gactgggagg ccatcgaatc ggcataccgg
     1081 gccggagtcc ttagcctccg tgatataggc gagaaatacg gcgttacaga aggggctatc
     1141 aggaagaggg ccaaaaagct tgattgggca cgcagtggcg gtacgcaggt ttgcaaaaat
     1201 ggtacgcaaa aaaggaaagt gcgtaccagc agaaagcctg ccattactgg ccttacacaa
     1261 aaaagtacgc aactaaaaac agaatctaca ccggatacga aaccgatacg cggaatgcgt
     1321 accgatcccc cgactaaccc attccaaccc ggtaaccagc aggcattaaa acacggtggt
     1381 tatgcccgtc gccttctgct caaagatgag gtgatagagg acgctaaagc gttgacgctc
     1441 gaggacgaat tatttcgcct tcgtgctaac aaccttgtcg ctgcagagaa tattggtcgg
     1501 tggttggtgt cgctggaaga tgctaatggg gaccaggaaa ggaagatgct gattgaaaat
     1561 atcagcgccg ccgagaaagc aatgatgcgc aatacagttc gtattgagtc catcgttggc
     1621 acgcttgcga cggtaggaaa aatatttgct gatacagcct accgcaaggc cgccactgat
     1681 aaggtgtctc tggaggctga tcgtcttcgc cgtgatgcag gtattgatga tggcaatgga
     1741 gagcgtgacc tcaatgactt ctactctgac atccaaaccg acgctgaatc cggctttacg
     1801 tagtttctgg actatgcggg cacgtaacaa agtgctttat ggtggtcggt catcgtcaaa
     1861 atcatgggat gccgctggca ttgccatatt tctgtcgaat aaatacaccc tgcgtttttg
     1921 ttgtgcccgt cagatccaga ataaaatcga agagtcggtg tataccctgc tcaaaattca
     1981 gatagacagg tttggtctgc ggcaccgttt ccgtattctg aacaacaaaa tcattaaccg
     2041 ggttactggc tcggaatttg ttttttatgg attatggcgc aacatcgaag aaattaagtc
     2101 actggagggg atcgatatgt tgtggttgga agaagcccac gcactgacgg aataccagtg
     2161 gaaaattctg gagccaacga tccgtaaaga gggttcggaa tgctggttca tattcaaccc
     2221 cggacttgtt actgatttcg tctggcgcaa cttcgttgtt gatccgcccg aaggcactct
     2281 catccgcaaa attaactatg acgaaaatcc gtttctgtct gacaccatgc ttaaggttat
     2341 cgacgcggcg cgacgccgtg atccggatgg ttttaaacat gtgtatgagg gcgttccgga
     2401 gtctgatgat gatgcggca
//
LOCUS       DAJTSF010000244         2373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000244 DAJTSF010000000
VERSION     DAJTSF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.253"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..823)
                     /locus_tag="OO335_004214"
     CDS             complement(<1..823)
                     /locus_tag="OO335_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6004350.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1424..1672)
                     /locus_tag="OO335_004215"
     CDS             complement(1424..1672)
                     /locus_tag="OO335_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6004351.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2027..>2373)
                     /locus_tag="OO335_004216"
     CDS             complement(2027..>2373)
                     /locus_tag="OO335_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6004352.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          611 a          437 c          576 g          749 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggtgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggg gcaatttttc cttaaggtat gaaaaaagga
     1081 gctgaagcta tataatagct attaatgcca ctggtatcag tgaatcatgt atataaaaaa
     1141 ctgtgttaag gaggctgtcc attagcggtt ttttattctt ctggaaccgc ggcatctttg
     1201 cgtatctttt tgtcttctca ccgtctggtc ggtgttttgc tgagactgct aatttcctgt
     1261 ctttgctagt gctgtactgg tactgcccaa tcatgattgg cgggggcggg cggagttgag
     1321 accgcagaca cgtcgtatgc aagaacgtgc tgcggctggc tggtgaactt ccgatagtgc
     1381 gagtattgaa tgatttccag ccgttactga ttttacgtgc taattagtga acaaaccact
     1441 cgtcagcaga ctcccaggta tctttcagag tttcctgaac aaaagtttta gctgaatctt
     1501 tatcggcggt gcgcgtaaca gaaaggccat cgttgctggt ggcttttacg atcacctcta
     1561 catcgtcata acgtttactg atgcgtcggg ttaattcttc ctttaacgca tccacagcac
     1621 cggttggcat tttagtcatt ttctctttgg ctatgcagat ttcaaaacgc ataaaaatcc
     1681 ctctgcactg tgtttttata cagtattatt tttaactgta tggataaaca gtgtcaagag
     1741 tgagggaaag tgattttttg gggcatgcat ggggcaaaaa tatttgttgt ggggcatttt
     1801 ggggcatgat tgggacactt tgttacatat gaactttgcc gattttcata tgacatggca
     1861 atccaacgct ttgattattc taaagaaaac acatgctctt gggcgttctt tagtgatttt
     1921 taaaaatccc gtatcacgca gttaaagtgg cgggcatact cttcaaggct ggtgatcccc
     1981 aaccgcaccc atttaggatg tgaccattgc ggtagaccga tgtaaatcat aacgcgtgaa
     2041 ggatctcttc cacgctacgc acacgggcga tgcgcgggta gatatgatta atgctgttat
     2101 tgtgttgctc ggcgctggcg gcactacagg cgtcttcggc aatcaccaga ttaaagccaa
     2161 gttcccaggc attgcgggcg gtggattcaa caccgatatt ggtcgagatc ccacataaca
     2221 ctattgtatc gatacctcgg cggcgcagtt gcaactccag atccgtaccg taaaacgcac
     2281 cccactgacg tttgatgatt tcgatatcac tgtcagttgc acctaatgca gctggatgtt
     2341 gccaccagtt ttcgggcaac actttagcgg ggg
//
LOCUS       DAJTSF010000245         2325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000245 DAJTSF010000000
VERSION     DAJTSF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.254"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            61..282
                     /gene="kil"
                     /locus_tag="OO335_004217"
     CDS             61..282
                     /gene="kil"
                     /locus_tag="OO335_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6004353.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYILHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            276..452
                     /locus_tag="OO335_004218"
     CDS             276..452
                     /locus_tag="OO335_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004354.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="OO335_004219"
     CDS             527..802
                     /locus_tag="OO335_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004355.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="OO335_004220"
     CDS             902..>2325
                     /locus_tag="OO335_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR6004356.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          720 a          531 c          528 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatattcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DAJTSF010000246         2305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000246 DAJTSF010000000
VERSION     DAJTSF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.255"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            726..2234
                     /gene="ltrA"
                     /locus_tag="OO335_004221"
     CDS             726..2234
                     /gene="ltrA"
                     /locus_tag="OO335_004221"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6004357.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          584 a          565 c          684 g          472 t
ORIGIN      
        1 gtcagttcgt ggcagaacga cctgaccagc tgtgggtggc tgattttact tacggtgtgc
       61 ccggagttca gggcgggcat ggatgcttaa atgaaccgcg agtctgtctg gaatattgaa
      121 ccggtaactc acgatgagaa acccaacaat cccaccgggt gtgacggtgg agaacctgag
      181 cggcagtgac ctgcggcatg cccgcagggt gatgtaaccc gctgacaacg gggattgagg
      241 cgagatcact aagccgagat gatcctcaag gttaagtact gaaaggctga agaacatgaa
      301 cccgttaatc cgcctctgtg ggttgaaaac gtcaccacgg cctacgtgat ctgacaggcc
      361 gtgcaggagg aactggcagt gatacgtaag cactgccggt cgaaggtgtt ttgacatgta
      421 tgcgaaacac cggggcagca gcgtctatca cacttgcgtt gctgacttct gccaacttgc
      481 ggcaagcaag gataaagagt gcgacgggca gcctcctcag tatgcctgag tccaggcagg
      541 taaaccgggg aaggtcagcg acggatgtta agggggcatg gctccgatga cgcgctggct
      601 ggcggagctt ccgtagtagt ccgcgatggg gaaagcccat tacatggcga agggaagcag
      661 tttgaatgtg tttgcgacgt gaattaactg acctaacgag gtgaagacct ttgataatca
      721 gcgaaatgca acgcaagctt gccacatggg cagccaccga tccgtcccta cggattcaac
      781 ggctgctgcg tctgataaca caaccagaat ggctggctga agcggcgcgg atcacgcttt
      841 catcaaaggg ggcccatacc cccggcgttg atggcgtgaa caaaacaatg ctacaggcca
      901 gactggctgt tgagctgcaa atcctcaggg atgaattact ctcaggccac taccagccct
      961 tgcccgccag acgggtttac atccctaaaa gcaacggcaa actgcgacca ctgggtatcc
     1021 ccgcgttgcg ggatcgtatt gttcagcggg ccatgctgat ggcgatggag ccgatatggg
     1081 agagtgattt tcatacgctc tcatatggct tccggcctga gcgcagtgtc caccacgcga
     1141 tccgcacggt gaaattacag ctcacagact gtggtgaaac ccggggacgc tgggtgattg
     1201 aaggcgacct gtccagttac ttcgacaccg tacatcatcg actgctgatg aaggctgtac
     1261 gccgcaggat cagtgacgca cgtttcatga ctctgctgtg gaaaaccatc aaggcgggac
     1321 atatcgatgt cggtctcttt cgggcggcca gtgaaggtgt gccacagggc ggtgttatat
     1381 cgccgctatt atcgaacatc atgctgaatg agttcgatca atacctgcat gagcgctacc
     1441 tgagcgggaa agccagaaaa gatcggtggt actggaataa cagtatccaa cggggccgaa
     1501 gtacggcggt cagagaaaac tggcagtgga aacccgcggt ggcgtactgc cgctatgccg
     1561 atgattttgt cctcatcgtc aaaggcacca aagcacaggc ggaagccatc agggaggagt
     1621 gtcggggtgt gctcgaaggc agtctgaaac tcaggctgaa catggataag actaaaatca
     1681 cccatgttaa tgacggcttt atctttctgg ggcacaggat cattcgcaaa cgcagtcgtt
     1741 atggcgagat gcgagtggtc tcaacgatcc cgcaggagaa agccagaaac ttcgccgcat
     1801 cgctgacagc actgttatca ggcaactaca gtgaaagcaa agtcgatatg gctgaacaac
     1861 tcaaccgaaa actgaaaggc tgggccatgt tctatcagtt cgttgatttt aaggccaaag
     1921 tcttcagtta tatcgaccgt gtcgtgttct ggaagctggc tcactggctg gcccgcaaat
     1981 accgtacagg tatcgcttcc ctgatgaggt ggtggtgtaa atcaccgaaa ccgggtcaga
     2041 gcaaaacgtg ggttttattt ggtaaaacca atcacggcaa gctcagcggc gaaatactgt
     2101 accggttggt ggggcaaggc aagaagctgt tccgctggcg gctacccgaa ggtaatccct
     2161 atctgaggac ggagaccaga aacacgtata catcgcgctt tacagaagtg gcaatggcgt
     2221 tcgccagcat ttaaatggag agccggatgc gctgaaaggt gcacgtccgg ttcggggagg
     2281 agaggcaggg aaatagtccg actac
//
LOCUS       DAJTSF010000247         2221 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000247 DAJTSF010000000
VERSION     DAJTSF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.256"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(504..2108)
                     /locus_tag="OO335_004222"
     CDS             complement(504..2108)
                     /locus_tag="OO335_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6004358.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          569 a          525 c          481 g          646 t
ORIGIN      
        1 aagacctata ggtttggtga ttgattatta taaatatcat caccccttaa ggtattccct
       61 tagagttaat ctttaattgg tctttaatta aatcttttat ttaatactta aagttaatct
      121 ctaattgtgt ctttaagtta actttaagta atactataag ttaatcttta attgtgtctt
      181 taagttaact ttaagttaag cccctgtagg gactagagac cataattaag atgattgtgt
      241 cttattgcta ttcacttatc gactgaccca gagcacacag gaagcattca ctttaagtag
      301 aacgcttggt gtctgtaatt ggtcatcccg cccctgaaag gatgcgataa gttacggtta
      361 gttgtcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta
      421 tccttgacaa gtagagaaaa ccatgagtca aaaacatgac cacacaggtc tgtagtaagt
      481 gtcatcctag actatggtct cctctaggtc tccttataaa catcagccat cacattgacc
      541 agcctatcaa cctcgacctc tttagcataa cggtcgtacg taatagaccc tgaactatgc
      601 cccatgatcg cctgtgcata cgccaactcg accccatgtt gttttaactg ggccgcaact
      661 gtatgtctaa acgagtgaaa cgctaagccg ctacggtcta cgaggacttt aggcaacaac
      721 gtccgattaa accatttact cacctgctca ccgtaaccgt tcttcatgag acgcagacca
      781 tcgaacacca tagcgtcatc accgtcagcg cctctacggg tctctacaag cgccataaag
      841 tcagccaata taaaaccata ggccccatcg accagaggca cacagcggtc actatgagcg
      901 ttcttgatgc tcttaccggg tagactacta tcgtcttcat tgatatgaat ataaacggtt
      961 cccgcctcag tggtcctgac gtctttgacc tgtagttggg ctatttcatt gagtctcgcc
     1021 ccagtgatag cagccaaggc ggtaacataa taatgatacg gcttaccaga ggtcttctga
     1081 gagtatgcct tagctgcgac cagtagttgc cccacctgct ccgtagagaa ggcgttacgg
     1141 gcccctgagg ctttacgctg agggacttta agctccagac cctcagtcat gttcttttta
     1201 atcaagtcat tgcgcacagc ccacctaaag acagcagcca tcttaataag atacttgttg
     1261 ttgatggtaa cgacatccag acaatctgtc ttatcttcac ggctcaacag gtcagcaagt
     1321 gggacatcct tgaagcgctg cttacggttc ctaggtaact gctggagcac atcacggacc
     1381 ctaagcatgt cagccctgtt agcgtctgca ttgagaccca gatggtcgaa tatttcgatt
     1441 aacgcagcgt gtgacgcctt gttttccctt aaggttgcag gcttccagtt ctgatagttc
     1501 tccgcttcgt attgttcaaa gaggctcgct atggtaatgc taggcgtaac ttctggtttt
     1561 acttctggag cattaacaga tagggacaca gagtctgctt ggttatcttg cctatcaata
     1621 tcattaaaaa gatcgacgta atctatcaga ggctgactgt ctcccgcttc gatacgcctc
     1681 atacaggctt ttagcacctt cagggcctca ttgatgtaac gatgctggtc aatgcttagc
     1741 ggctcactag ccagagcatc ggtaaggctc tcaccaagtt ctccgtattg gtcccggtag
     1801 atatccctca tgtcaggctc aaagagatct gaacggccca tagacagttc acactcagcg
     1861 atgtccttaa ggtgttccct catttcctca taggtagcgt tgggtctgtc tgcgtgtatt
     1921 gccttgagtg ctgcctttat gtgccttgag taagccatag cggtgcgtct atccttggtc
     1981 cgtaatgata ttgaggtcat tctatcattg tttgattgtc tgaccctaag acggaagtag
     2041 tagacgccgt tacgctggta gagatagtag actgctggaa gtttgtgact actggatagc
     2101 tgaatcatat gtctgcatgc cctgagaggg ttacacagtg ggttacagta agtaactctg
     2161 caaggaggct taaagccgta tcccttagga ttcagtaggt tggagtactt taagaaaggt
     2221 c
//
LOCUS       DAJTSF010000248         2218 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000248 DAJTSF010000000
VERSION     DAJTSF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.257"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="OO335_004223"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="OO335_004223"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR6004359.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="OO335_004224"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="OO335_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6004360.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..>2218)
                     /gene="arsR"
                     /locus_tag="OO335_004225"
     CDS             complement(1881..>2218)
                     /gene="arsR"
                     /locus_tag="OO335_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6004361.1"
                     /translation="IQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRH
                     LALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNC
                     SGDSKNICS"
BASE COUNT          589 a          611 c          555 g          463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgg
//
LOCUS       DAJTSF010000249         2201 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000249 DAJTSF010000000
VERSION     DAJTSF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.258"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(135..401)
                     /gene="ospE2"
                     /locus_tag="OO335_004226"
     CDS             complement(135..401)
                     /gene="ospE2"
                     /locus_tag="OO335_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HCR6004362.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(583..984)
                     /locus_tag="OO335_004227"
                     /pseudo
     CDS             complement(583..984)
                     /locus_tag="OO335_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1413..2201
                     /gene="virF"
                     /locus_tag="OO335_004228"
     CDS             1413..2201
                     /gene="virF"
                     /locus_tag="OO335_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR6004363.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          677 a          389 c          408 g          727 t
ORIGIN      
        1 gtatcgagga tagatggagt tactcctctc tgaaatagaa ttactgctct actttttctg
       61 tttccagaaa ttattagtaa caaaccctga tatattcttt tgaaaatgga gcttaaacga
      121 gtctgaaatt aacttcagaa atcagaatac cgttggcttt gtttttcatc aattccggct
      181 tcatttatct ttgtcaacca ttgcatttct gtagcagtga atttcaacgt tttcgtccgg
      241 ttggcagcag gtatttgtaa ttcgtatata gctgcttttt tattgaaaac agtatatcca
      301 tctgtagtaa cagtagaaga taataataca ggatttgata cttcaaggat gatattttct
      361 tgtttttgtg gaaggcaagg gaatatagtt tgtgtaagca tattgcttcc ttatgttata
      421 ttttagtaaa gaatttattt tttacattat agtcggctaa atgaagttta ctgtaaaaaa
      481 acgatacttt tttcgtaagc gatgagatgt ggagccagaa aaatggctgc gttacgtcat
      541 tgaactgtcc ttgacccacg atattcagac agtcgttgta tcttaagttg acattacccg
      601 ctgacgtcga tattcccttg gtgagagata acccaacgca ctatgcggat ggttttcatt
      661 gtaatgctcg atcgccactg caaggttatg caatgccgtt cttggtttcg gcatgaacgc
      721 gatgcagtct tccttcatcg ttttcatgaa ccgttctgct attccattac tctgagggct
      781 actgactgcc gtcgtgcagg gctccagatt caactctctg gcgaactgcc gtgtttcatg
      841 agccgtataa gccgaaccgt tatcggccac tggattggcc gttccggtcc tttatcgcca
      901 tatcgctcct gacatgacat cctgcaccgt tgctttgtca taatctcccg tgcctgctgc
      961 ccagtctatg gcttcacgat cgcaatatgg aacgtaacgc tgtcatcggg agaatcgtca
     1021 atgcggcatt cggttaacgc ttaccaatat caacaccaac cggatttgtg gagcctccag
     1081 tctgaagggg ctttatgcgt tccgtatagg atattatgat gctggagttt tgcgaagctc
     1141 cactcctttc cgaacattta cacagatatt gcctaagaaa agtgaaacca taatatggtt
     1201 atagtccctt tcagtgcaaa tacttagctt gttgcacaga gaaatagaag ctgcataagc
     1261 tctttcttca aaaaatgtaa ataaagttaa atataggaaa aattacttaa tctatcttaa
     1321 taacggaaag attttgtata caatcactgt tacagaaatt tcttagttac tctgtaaaca
     1381 ctaaatatag tttggtatat tctgttgaat ttatgatgga tatgggacat aaaaacaaaa
     1441 tagatataaa ggttcgcttg cataactata ttattttata tgcaaaaagg tgttcaatga
     1501 cggttagctc aggcaatgaa actttgacta tcgatgaagg gcaaattgct tttatagagc
     1561 gaaatataca aataaacgtc tccataaaaa aatctgatag cattaatcca tttgagatta
     1621 taagccttga cagaaattta ttattaagca ttattagaat aatggaacca atttattcat
     1681 ttcaacactc ctattctgag gagaaaaggg ggttaaacaa aaaaatattc ctcctctctg
     1741 aggaggaggt ttctatcgat ttgttcaaat ctataaaaga gatgcctttc ggcaaaagaa
     1801 agatctatag tttagcttgc cttttatcag ctgtttctga tgaggaagct ttatatactt
     1861 cgatatcgat agcttcttct cttagttttt ctgatcagat aaggaagatt gttgaaaaaa
     1921 acatcgagaa gagatggcgt ctttctgata tttcaaataa cttgaattta tcagaaatag
     1981 ctgttagaaa acgattggag agtgaaaaat taacatttca acaaatcctt cttgatattc
     2041 gcatgcatca tgcagcaaag cttttattga atagtcaaag ctatattaat gatgtatcaa
     2101 gacttatcgg aatatcaagc ccatcttatt ttataaggaa atttaatgaa tattatggta
     2161 taactccaaa gaaattttac ttatatcata aaaaatttta a
//
LOCUS       DAJTSF010000250         2196 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000250 DAJTSF010000000
VERSION     DAJTSF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.259"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            73..378
                     /locus_tag="OO335_004229"
     CDS             73..378
                     /locus_tag="OO335_004229"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6004364.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="OO335_004230"
     CDS             complement(454..918)
                     /locus_tag="OO335_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6004365.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OO335_004231"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OO335_004231"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6004366.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..>2196)
                     /locus_tag="OO335_004232"
     CDS             complement(1787..>2196)
                     /locus_tag="OO335_004232"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative N-acetylmannosamine-6-phosphate
                     2-epimerase"
                     /protein_id="HCR6004367.1"
                     /translation="AQAGADIIAIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPED
                     GLACQKLGAEIIGTTLSGYTTPETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADA
                     MRHGAWAVTVGSAITRLEHICQWYNTAMKKAVL"
BASE COUNT          535 a          622 c          537 g          502 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcagaatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgcca
//
LOCUS       DAJTSF010000251         2187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000251 DAJTSF010000000
VERSION     DAJTSF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.260"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(220..384)
                     /locus_tag="OO335_004233"
     CDS             complement(220..384)
                     /locus_tag="OO335_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004368.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="OO335_004234"
     CDS             1286..1570
                     /locus_tag="OO335_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6004369.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="OO335_004235"
     CDS             1558..2043
                     /locus_tag="OO335_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6004370.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          462 c          620 g          540 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatgccc cctataa
//
LOCUS       DAJTSF010000252         2183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000252 DAJTSF010000000
VERSION     DAJTSF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.261"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..694
                     /locus_tag="OO335_004236"
                     /pseudo
     CDS             1..694
                     /locus_tag="OO335_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            696..1301
                     /locus_tag="OO335_004237"
     CDS             696..1301
                     /locus_tag="OO335_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR6004371.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1288..>2183
                     /locus_tag="OO335_004238"
     CDS             1288..>2183
                     /locus_tag="OO335_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6004372.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          507 c          609 g          536 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat
      361 cagctggcag acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga
      421 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
      481 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      541 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      601 tggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      661 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      721 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      781 tttcccccga cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag
      841 aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa
      901 aggacgattt taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg
      961 atccctgtcc gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg
     1021 acctgtctga tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg
     1081 actgttccgt tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg
     1141 cttatgttct gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt
     1201 tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg
     1261 tttcagtagt tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg
     1321 gtcccctttg cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc
     1381 agagagagcg gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc
     1441 tcagcgggag gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc
     1501 gcagcaatac ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg
     1561 gccattggtg gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg
     1621 ctgaatacca cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta
     1681 aatcttcttg aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt
     1741 cagaataaag ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa
     1801 cgctatgttc atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat
     1861 gcgctgagga ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg
     1921 caccttattc ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg
     1981 ttcaccatta atttgcggac gggtgaaata tccatgtcgc ataaagtgtc tgttggcggt
     2041 ggttcgcagg tcaatggtgc gctgggtatc ggcgttcaga acgccctggg ggggaattca
     2101 attgttcttg gtgataatga caccggattt aaacagaatg gagacggtat tctggatgtt
     2161 tatgctaata gtcagcgtgt att
//
LOCUS       DAJTSF010000253         2162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000253 DAJTSF010000000
VERSION     DAJTSF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.262"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            80..259
                     /locus_tag="OO335_004239"
     CDS             80..259
                     /locus_tag="OO335_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004373.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            311..505
                     /locus_tag="OO335_004240"
     CDS             311..505
                     /locus_tag="OO335_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004374.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1304..1640)
                     /locus_tag="OO335_004241"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="OO335_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          688 a          444 c          450 g          580 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctgacgaa atcaacatga aaataaccat atatgataat tattataata acggctttaa
     1801 ttggaataca tatattacaa cgtattatat ataattggta ttctgggaac tatattctca
     1861 aaatacagta gaaacgaggt atgtttctgg tggaaaggac agtgggatta aaaagtaaga
     1921 attgataaaa aaaacgccag caacacatag tgccggcgga gggaataccc catggagaaa
     1981 atgtgatgcc tagagcatca tgaatatata attaaaaata gttagcgttg tcaatacttt
     2041 cacaaaaata attttcgtag tatagaaaga tatttttatg catgacctac ctgaatttgc
     2101 tccgggtaga ggttataaat aaaaattgaa tcacgacaaa cacaatattc atagtatggc
     2161 ga
//
LOCUS       DAJTSF010000254         2151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000254 DAJTSF010000000
VERSION     DAJTSF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.263"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO335_004242"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO335_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR6004375.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="OO335_004243"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="OO335_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1982)
                     /locus_tag="OO335_004244"
                     /pseudo
     CDS             complement(1292..1982)
                     /locus_tag="OO335_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          630 a          398 c          493 g          630 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttag ttgtaattta
      901 tttatggagg ttttctatgg aattcttttt ttattgtggc tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aagccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgaca aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt caggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gtcctggcgg tatcacggcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctta acgccgagtt ttctggcttc ataagagtaa
     1861 gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta
     1921 cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc
     1981 atggaaaacc tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct
     2041 tttgccgcaa taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa
     2101 aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata a
//
LOCUS       DAJTSF010000255         2122 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000255 DAJTSF010000000
VERSION     DAJTSF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.264"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..132
                     /locus_tag="OO335_004245"
     CDS             <1..132
                     /locus_tag="OO335_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR6004376.1"
                     /translation="LKVDPLKCELRGGQMRFTGLKRGYRLAEQVMMHEPLARIRWCG"
     gene            319..556
                     /locus_tag="OO335_004246"
                     /pseudo
     CDS             319..556
                     /locus_tag="OO335_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            977..1555
                     /locus_tag="OO335_004247"
     CDS             977..1555
                     /locus_tag="OO335_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004377.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMKHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1664..1922)
                     /locus_tag="OO335_004248"
                     /pseudo
     CDS             complement(1664..1922)
                     /locus_tag="OO335_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1906..2122
                     /locus_tag="OO335_004249"
                     /pseudo
     CDS             1906..2122
                     /locus_tag="OO335_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          671 a          375 c          516 g          560 t
ORIGIN      
        1 ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt tacggggctg
       61 aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc tcgaatacgg
      121 tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa cggagcaaaa
      181 aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct ataccgatta
      241 cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt aatcttttgg
      301 ggatttaaat tcctatcgat gaactgtcga tatgaaaaag cgagcatcat actgacgtca
      361 aacaaagggg tcgcagactg gggagaaatg ttcggagatc acgtgctggc aacagcgatc
      421 ctagatcggc tgctacacgc tgaatatcaa aggagagagt taccggttaa aagagaaacg
      481 taaagctgga gtgctgacca aaaacacaac gccaatcagt gatgatgaaa tggtggaaaa
      541 cggacagcat cagtaacgaa agtatcttag caggcatgaa aatcgtccgc atcccctaaa
      601 cggtcaaaat gcttctgccg tgaaggaagg agaatgccat ctcatcactt atatctggcg
      661 aatgaaacac agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa tggaatggca
      721 gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg ttatgttcga acctgacgtg
      781 ttaacagcag taaagtagtc tcgcgaaggt gttcaaacat tataatgaat ggcatcctca
      841 tagtgcgcag ggttatcgct tgctgcggaa atatctgaag cgacgtacca gtaatgggga
      901 aaatgatagc tggtttctga aaatatagag gaaaatgtga tatatatatc ctgttaatat
      961 ttaaggagaa tataaaatga aagtctcgtt taagtcttta ggctatattt ttcatgatat
     1021 atataataaa aaacacacta tcgatgagtt caacgatgtg gtaagaaaag cagttttatc
     1081 aggtaaaatt aacgagctga atgcatgtca taaagtggca atttttcttg ccgaaaaaga
     1141 caatgagata acaaagaaag acaaagccaa gataatagat acattaactg aaaactattc
     1201 cattgaattt caacaattaa tgaatattag tgagcgcacg ttaaattcat ctttatatat
     1261 tactcctggt gaaagtggtt ttgtcagttt tgttaatcgt gaaggaaaaa tatgtcatac
     1321 tgcatatgta aaatcatcag ataatagcat gacatattac catgcaaatg gtagcagcat
     1381 tgacaaatat attacggata tgtgcggcct tatttgtatg aagcatattg agtctactgg
     1441 cattattttt tatatgcttg atgaaaaagt tttatctgcc attgctgaat ttatgaatga
     1501 aaaaggatgg cgggctgcgt tttgttcagc aaagaactta tataaatgtg tttaatttta
     1561 gaaaaactat ttggtaaaat ccaacaagag tcgagaggtt cgcgttgctt cagcagggcc
     1621 tcagcctgtt caaccagact cagttgcagg tcgtgacgca gatttagtgg tggtggttga
     1681 ttgtcagata ccagccgatg atcttgtagt gcatatcatc agactttgag aagcatatgg
     1741 ttttgcgggt aagccgctta agatgtgtcc gcaggttcag attatgtcgc tctatccgct
     1801 gagtgcattt cttgcttata acatgactgg ttgaggacaa taaagttcgg tataccggct
     1861 caggcatcgg tcatgtagaa ggcaatgcta aactggctca gcaaactaag agcagcgcgc
     1921 atccacttac ccaatgcggt tgagaaaata gccttcgatc gctttcatgt ggcgaaacaa
     1981 ccgggtgagg tggtggataa aacccgccag aatgaacatc cgcatcttcc tgttgaaagt
     2041 cgtcgtcagg caaagggaac tcgcttcctg tggcagcaca gcgataaatg gatgaccgaa
     2101 tccaggcagg aaaagctgat at
//
LOCUS       DAJTSF010000256         2092 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000256 DAJTSF010000000
VERSION     DAJTSF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.265"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="OO335_004250"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="OO335_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6004378.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="OO335_004251"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="OO335_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR6004379.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          559 g          512 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tt
//
LOCUS       DAJTSF010000257         2085 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000257 DAJTSF010000000
VERSION     DAJTSF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.266"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..797
                     /locus_tag="OO335_004252"
                     /pseudo
     CDS             1..797
                     /locus_tag="OO335_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            912..1253
                     /locus_tag="OO335_004253"
                     /pseudo
     CDS             912..1253
                     /locus_tag="OO335_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1236..2073
                     /locus_tag="OO335_004254"
                     /pseudo
     CDS             1236..2073
                     /locus_tag="OO335_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          515 a          504 c          631 g          435 t
ORIGIN      
        1 tgggccttgc ccgtagcagt tatttttatc accgggctgc actgaaagcc ggtgataaat
       61 acgccacgat acgtaagatg ctgacagata tctttaacag taattaccag tgttatggct
      121 atcgtcgcct gcatgcgatg ctcaggcatg agggggggcg gctatcagaa aaggttgtac
      181 gcagacttat ggtggaggaa cagcttgtcg tcagtcgtaa ccgtcgtcgt cgctacagct
      241 catattgcgg agaaatcgga ccggctccgg ataaccttat cgccagagat tttaaggcgg
      301 agcaacctaa tcagaaatag ctgacagata tcacggagtt ccagctccct gcaggtaaag
      361 tctggctgtc accagtggtg gactgcttcg atggaaaagt tgtgagctgg tctctcagta
      421 cacgccctga tgctgaactg gtcaacacta tgctggatag cgctgtcgaa acgttaaatg
      481 ctggcgaacg accggtgata cacagtgaca gaggtgggca ttatcgctgg ccaggctggc
      541 tggaaagagt gaatgcagca ggtcttattc gctcaatgtc ccgtaaagga tgttcacctg
      601 ataatgctgc atgcgaaggc tttttcggca gactgaaaac ggaaatgtat tatgggcgta
      661 aatggtcggg catcacgcca gaaaagttca tgcagcaagt ggatgcttac atcagatggt
      721 ataacgagcg gcgtataaaa ttatcgctgg gtgccgtcag ccccaaaatg taccgccaac
      781 aatgcgggct ggaatgataa agcagtccag gaaatcgtcc gcatcccccc ttcaccattt
      841 ggattgtccg ctccacccaa catgttgttt ccttaaggtt cctacaccac aaaggaaact
      901 ctcatgctgc gcagtggatg gctgacgttg ccgacaggcg ggaacttcgc cagttcaggc
      961 agacaccgga acagcgcttc acgcaggaac aggagcatct gcagccgctg ctgggtacgg
     1021 acttcgatat ccgtcatgtc tcctgggatg gctatatcga ggttggcggg aaccgttaca
     1081 gtgtgccgga aagcctgtgt ggtcagctgg tatcaatacg gatctcgctg gatgatgaac
     1141 tgcggatcta cagtaatgag caacaggtag catcgcaccg tctctgttcg gctgcatacg
     1201 gctggcagac cgtgccggag catcacgctc cactctggca gcaggcttcg gagcgcatgg
     1261 ccgaatggct gggggagata cagaaacggg taatcactgt ctgtgaccgg gaagcggata
     1321 tctggcatta cctgtattat aaagtcagcc acggacagcg tggtgcgtgc tgcacagaat
     1381 cgccggctgg aagaggcacc cggcaagctc ttcgaactgc cggaagtcct ggcaaccgca
     1441 ggaagccaca cgctgaatgt gatgcaaaaa ggcgggcggg cagcccgtca ggcccggatg
     1501 ttcatcagtt acagcgaagt cagcataaaa aatcccgaca acagcggcca ggcgctcccg
     1561 ctcacgtatg tctgttgccg ggagcaggca gaggacggtg cctgctggca tctgctgacg
     1621 tcaggaaaag cggcgagtgc cgccgatgca cgacgtattg tcagccatta cgagcgacgc
     1681 tggctgacag aggaatacca caaggcgtgg aaaagtggtg gtacatggaa tcgctgcgaa
     1741 tgcagacccg ggataacctt gagcgcatgg tggttatcct ggcgtttata gcggtacggg
     1801 tgctgggcct gcggcaggga ggtgtcagtg aagaaacaca aaatgacagc tgtgagaaaa
     1861 tactgacgcc gacagagtgg aaactactgt gggttaagct ggaaggaaag ccgcttccgg
     1921 tgcaggcacc gaccctgaaa tgggcctggg gcgatggcat gacagcaaac gcacaggtcg
     1981 tcccggttgg ggcgtcatgt gggatggctg gtcagacttc aggatatggt tgagggttac
     2041 ctggtgatga agtctcttga tcaggagctc tgatcaagag acagc
//
LOCUS       DAJTSF010000258         2032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000258 DAJTSF010000000
VERSION     DAJTSF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.267"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..362)
                     /locus_tag="OO335_004255"
     CDS             complement(<1..362)
                     /locus_tag="OO335_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6004380.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /locus_tag="OO335_004256"
     CDS             complement(429..1643)
                     /locus_tag="OO335_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR6004381.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          633 a          492 c          413 g          494 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttgaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat tcaattaatt aaaatgtgta ttgcttgatg
     1921 tatttaaaaa tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag
     1981 gaatgtgaca aataatggtt ctggttgggc gttaaattta aggtcaccag aa
//
LOCUS       DAJTSF010000259         2030 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000259 DAJTSF010000000
VERSION     DAJTSF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.268"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..2030
                     /gene="sat"
                     /locus_tag="OO335_004257"
                     /pseudo
     CDS             1..2030
                     /gene="sat"
                     /locus_tag="OO335_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          746 a          356 c          398 g          530 t
ORIGIN      
        1 tacggcatga tgatttataa aaatgataaa acatttcgta atcttgaaat attcggagac
       61 agcggctctg gagcatactt atatgataac aaactagaaa aatgggtatt agtcggaaca
      121 acccatggta ttgccagcgt taatggtgac caactgacat ggataacaaa atacaatgat
      181 aaactggtta gtgagttaaa agatacctat agtcataaaa taaatctgaa tggcaataat
      241 gtaaccatta aaaacacaga tataacatta caccaaaaca atgcagatac cactggtact
      301 caagaaaaaa taactaaaga caaagatatt gtgttcacaa atgggggaaa tgttctgttt
      361 aaggataatt tggattttgg ttgcggtggt attatctttg acgaaggcca tgaatataac
      421 ataaacggtc agagatttac atttaaagga gcaggaattg atatcggaaa agaaagcatt
      481 gtaaactgga atgcattgta ttccagtgat gatgttttac acaaaatagg ccccggtact
      541 ctgaatgttc aaaaaaaaca gggggcaaat ataaagatag gtgaaggaaa tgttattctt
      601 aatgaagaag gaacatttaa caatatatac cttgcaagcg gaaatggtaa ggtaatacta
      661 aataaagata attcccttgg caatgatcaa tatgcgggga tattttttac taaacgtggt
      721 ggcacgctag atttaaatgg acacaatcag acttttacta gaattgccgc cactgacgat
      781 ggaacaacaa taactaactc agatacaaag aaagaagccg ttctggcaat caataacgaa
      841 gactcctaca tatatcatgg gaacataaat ggcaatataa aactaacgca caatattaat
      901 tctcaggata agaaaactaa tgcaaaatta attctggatg gtagtgtcaa cacaaaaaat
      961 gatgttgaag tcagtaatgc cagtcttacc atgtaaggcc atgcaacaga gcatgcaata
     1021 ttcagaagca cagcgagtca ttgctccctg gtatttcttt gtggaacgga ctgggtcacc
     1081 gttttgaaag aaacagagag ttcatataat aaaaagttca attctgatta caaaagtaat
     1141 aatcagcaga cctcatttga tcagcctgac tggaaaaccg gggtgtttaa atttgataca
     1201 ttacacctga acaatgctga cttttcaata tcacgcaatg ccaatgttga aggaaatata
     1261 tcagcaaata aatcagctat cacaatcggc gataaaaatg cttacattga taatcttgca
     1321 gggaaaaata ttactaataa tggttttgac ttcaaacaaa ctatcagtac taatctatcc
     1381 ataggaaaaa ctaaatttac aggtggcatc actgcacata acagccaaat agccataggt
     1441 gatcaagctg tagttacact taatggtgca acctttctga ataatactcc tataagtata
     1501 gataaaggag caaaagttat agcacaaaat tccatgttca caacaaaagg tattgatatc
     1561 tccggtgaac tgactatgat gggaatccct gaacagaata gtaaaactgt aacgccgggt
     1621 ctccactacg ctgctgatgg attcaggctg agtggtggaa atgcaaattt cattgccaga
     1681 aatatggcat ctgtcaccgg aaatatttat gctgatgatg cagcaaccat tactctggga
     1741 cagcctgaaa ctgaaacacc gactatatcg tctgcttatc aggcatgggc agagactctt
     1801 ttgtatggct ttgataccgc ctatcgaggc gcaataacag cccccaaagc tacagttagc
     1861 atgaataatg cgatctggca tctaaatagc cagtcatcaa ttaatcgtct agaaacaaaa
     1921 gacagtatgg tgcgttttac tggtgacaat gggaagttta caacccttac agtggacaac
     1981 cttactatag atgacagtgc atttgtgctg cgtgcaaatc tggcccaagc
//
LOCUS       DAJTSF010000260         2029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000260 DAJTSF010000000
VERSION     DAJTSF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.269"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1824)
                     /locus_tag="OO335_004258"
     CDS             complement(<1..1824)
                     /locus_tag="OO335_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR6004382.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2029)
                     /locus_tag="OO335_004259"
     CDS             complement(1932..>2029)
                     /locus_tag="OO335_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6004383.1"
                     /translation="WNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          485 a          579 c          430 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagc
//
LOCUS       DAJTSF010000261         1997 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000261 DAJTSF010000000
VERSION     DAJTSF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.270"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..345)
                     /locus_tag="OO335_004260"
     CDS             complement(<1..345)
                     /locus_tag="OO335_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6004384.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGND"
     gene            complement(342..875)
                     /locus_tag="OO335_004261"
     CDS             complement(342..875)
                     /locus_tag="OO335_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6004385.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(941..1570)
                     /locus_tag="OO335_004262"
     CDS             complement(941..1570)
                     /locus_tag="OO335_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004386.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1574..1789)
                     /locus_tag="OO335_004263"
     CDS             complement(1574..1789)
                     /locus_tag="OO335_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6004387.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          507 a          532 c          439 g          519 t
ORIGIN      
        1 atcattgccc atgccggagg cgctggaggt ttcggctgag gatggcacag ggcattttcc
       61 tttgacgagc acccgaccac cattatcaag cttgcgccga agagcatcat tttcagcttt
      121 cgcatcagct aactccttcg tgtatttagc atcgagtgca tcagcatcac gctggcgctg
      181 ctgcatgtca gtaatggttg cgttcgccag cttcagttct ccggcgtttt tgtcgcgctg
      241 ctctttgtag gtaatggcgt tatcacggta atgattaaca gcccatgaca ggcagacgat
      301 gatgcagata accagagcgg agataatcgc ggtgactctg ctcattgatc tatcccccaa
      361 caggctaatg cgctttcctg gtcacgacga ataacctgtc catagcagtt atttgaacgt
      421 atgcggcaat cgcgcccaac atcttttatc caccagcgaa tcgcctcgca tgcgccctta
      481 cgatcaccgg cattcagccg cttataaaac gtcgacggga aacacttacc ggggccaatg
      541 ttataggggc aaaatgacgc tatacccgct ttctggggtt cggtcagagg tactttaata
      601 ttgcgctcca cccatgccag cgccttatca cgttcaatag cgttaacctg gtcacatttt
      661 tccttcgaca gcttcattcc cggtatgaca ggcttaccat ccaccattgt ggcaccacga
      721 cagatggtcc atatgccaga accatcacgg tatgccgttg tgtgattacc ctctttttcg
      781 ttcagaaact ggtcaagtat ctggggagca gacgcgcctg cggcaatcag cgccagaaca
      841 gcagccgaca ggccgtattt gatttttgcg ctcatggata tttatcagga tttatcggca
      901 acaaataacg agccaactta tatacgcctt ttaagataag tcagccccgg ctggatccag
      961 tcatcaggct ctttcttaaa gggggagtat tgaaatcacg aagaagagcc tcccgcaccg
     1021 cagcatccat gtctgcacca ctggcaagtg cttcaatctc tgccgccacc tgcaaatacc
     1081 ccatgcaacg gccaatgcgt ttcataactc tggtgttttc gtctttcagg catctgatag
     1141 cgagattaat aattttcagg cagtgatgat gcgatgcccg gacaggtttt ccgttctcat
     1201 aaacgacgaa ctcaccctct tcacaggaaa ccctgaaagg ccgatggggt tcctggtctt
     1261 tatccggtgc agacgcaata aaatcacttt tatgctcttc ctctccggca ctgttaaccg
     1321 taatgacgta ctcaggccgc aggtaagccg cctctttttc tgacaatgcg gccggtaaaa
     1381 acttttcctg caaaaacttc ataaaagtct cgtgagcgac tttgtggcag taatcgtaaa
     1441 tcgccgcata atgctcatcg cggcgtttgt tttcatcttc agaactcacc aacgccgaca
     1501 attttttatt aagctcagtg atttcatttt ccaggtgatt gaatcgctga ttcatttctt
     1561 catgcttcat tatttactct ccccgtgcag ccttacgctt gtcttctctt attttgaaat
     1621 acagattcgt cagataagtc agaagcccca gaagcagact tcccagcaca ccaatcgcag
     1681 cccactgtga tggcctgacc tgatccaacc actgcaaaaa ccagtagccg gcactgccgg
     1741 cggaggtgcc gtaggcaatg cccgttgaaa ttttgtccat ggatttcata gcctcacctc
     1801 cgcaaataac ggatggcgta gttttacact gagaaatgaa agggatttga aaagaaaaaa
     1861 acgcaaaagc gggcgaaacg atatatacag taaggaaagc actctatcca acaaaccacc
     1921 cacagttaat cggaataaaa gcagagtgct tatgaatgat cgcacgcccg aaggttagta
     1981 tttctgcaca gcaattt
//
LOCUS       DAJTSF010000262         1982 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000262 DAJTSF010000000
VERSION     DAJTSF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.271"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            141..445
                     /locus_tag="OO335_004264"
                     /pseudo
     CDS             141..445
                     /locus_tag="OO335_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            614..1978
                     /locus_tag="OO335_004265"
     CDS             614..1978
                     /locus_tag="OO335_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6004388.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
BASE COUNT          501 a          392 c          463 g          626 t
ORIGIN      
        1 tcccggccct gccaagccat acccgctatc ggcgatgaaa atgcttttgt gcgcggtcgg
       61 agtggtcccg acgagggttt tacccgaagt cggggcgtat ctccgcgtta gcgggccgtg
      121 agggccgctt acgagcgtgt atgagagacg tacagtggtt ttgtccgctc acaccagtac
      181 atacaggtac ggaaataaaa ccagtcgaaa tgccggtaac gacgattact cctgtctctg
      241 atgccggtgg actacaagat tttatttact ggcgtcctga tgcggacagg gctggtatgg
      301 aacctgttta tgtgatactt aatgatcctc tggattccgg gcgatttacg cgtaaacaac
      361 taaataaaaa tatcttaaac atgcaagtaa ttttggtatt ctcgatacaa gaataaatag
      421 tagtaactac tatagctgca aataatgtta aaaacatttc tatgatcatc tttacatttt
      481 tcatgattga caattgtttt tggttaaaaa aagacaacta atgagtgtga taatgcaata
      541 ctttgagtga ttatataatg ataagtaaac aggtttgcga caattggtta tcagtattac
      601 agaaggatta aatatgtcat tattcacaga acctaaggaa attgaaagat taccctctga
      661 agagattgaa cgattatatc ccgtcctgcg atacagggtc tttatcagca ttttcctcgg
      721 atatatgggg tattattttg tccgaaacac caccagtgtg ttatccggtg tgctgcacat
      781 gtctgcaacc gagatcggca ttatctcctg tgccggtttt ttatcctatg gtatatcaaa
      841 gtttgtttcc ggacttattt ctgacagaag taattcaaaa gtatttttgt ctcttggttt
      901 attcctgtcc ggactggtaa attttctgat aggatatatt cccgggatca tcacatccgt
      961 tacgttattt tcaacaatgt atttacttaa cggctggatc caaggtatgg gatatcctcc
     1021 tggtgccaaa acactggtgt tctggtatga acatcgggaa aggatcacct gggccacact
     1081 gtggaattta tcgcacaatg tcgggggagc tctggccccc gttctgattg gtttcagttt
     1141 tggttttttc ggcgacagtg ctctggatca tgcccgggcc gcatttatct tcccaggagt
     1201 actgtgtatg gcaatgagtg tgctgattta ctttattcag gtggacagac ctgtatcagt
     1261 ggggcttcct cccattgaag aatggaaagg taacgtcgtt tcacacccgg ctaaaggccg
     1321 tgaacaaggt ccgagactct ccattcctga tatcatcaga aaacatatca tccgaaataa
     1381 taaactcatt tactgctgta tctatggttc gtttgtttat attctgagat acggtattgt
     1441 ttcctgggct cctaagtttt taagtgacag tctggatgtc ggtggtaagg atatgggaaa
     1501 actggcatca atgggggggg gttctgtttt tgaaatcggt ggtgtggctg gtatgcttct
     1561 tgccggatac ctgtctgtca gactgttcag aaactcaaaa ccattaacaa atactttatt
     1621 tctggcactt accattattt tattaatcgc gtactggtac gtgccatcag gcaatgaata
     1681 tctctggcta aactatacaa tccttattct gttggggctg gcagtgtatg gtccggtgat
     1741 gtttattggt ctttattcaa tggagcttgt tcctaaagaa gctgccggag ccgcatcagg
     1801 gctgtcaggc acattttcct atatcttcgg ctcaattgtt gcgacactgg gaatgggact
     1861 tgttgtcgat tatctgggat ggggggccac atttatcgta ctgatactgt ctgctgtttt
     1921 tgccatcatt ttcacactga tgagtcgtga acgctcactg gagttcgaaa aagagtaatt
     1981 tt
//
LOCUS       DAJTSF010000263         1976 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000263 DAJTSF010000000
VERSION     DAJTSF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.272"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..837)
                     /locus_tag="OO335_004266"
                     /pseudo
     CDS             complement(1..837)
                     /locus_tag="OO335_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          533 a          465 c          457 g          521 t
ORIGIN      
        1 agctgctcta cggtcatatt tttaattgcg ctccggttta caagagccca cccctgtttt
       61 tccagataaa actggaaagt atccagggta cagaccagtg caccgtcagg aacagtttcg
      121 gtgaatctga tattgccgtg ttcgtcgaag tgcacaacca gagtgcgacc gttacccgga
      181 atcatcttgt cagcggacgg ggtattattc tggtgcagtt ctgcctccat gcggtcgaac
      241 atcagcgatg taggcctctt tgaatgcagc ggcttttttc ccagtgaagc ccatcaccag
      301 gaaaacgaag ccgtttttgg tgatttggta cattgggcgt ttttcgcctt tggtgtcggt
      361 gtaggtgacg tcctcaaaat tgagggcgtt aaattcaggt gagcattcag tatttgcgat
      421 ggcacgtaac acattgtcgt gtcgtttgtg gaagaactct gcaaccgcaa cagacgtagt
      481 gacagcgcga ccgttttcga tggttacgtc agggtgagaa agggcaggga tagtagccat
      541 gatggcatcc tcgagtgata agttaattaa ctcaccaccg aggttttcca cgaccataag
      601 ggtggtgaga cgtacagggg tggaaatacc ggtcactcga gaacccggcc agtcttgcga
      661 ctgccctgca catcccacca taatttgaat ggagctgtgc attacgcata aaaaaaccgc
      721 ttcagcgcgg ctatgcgctc gagtaacgtt cgggtttcca cgcccggccc ccgttttatg
      781 aggtgcagat gcactataat tccacccgtt ctggttttca atagctacat tcaacatttt
      841 ctcttacctt tcatcaccga agtgaacttt gttgatgcgg tgcctggtgc ctccaggtga
      901 cgttaaccag ttaacaatta acgccggata cagggaaacc cataaccacc cgatacgtgg
      961 gaactaatcg ctttttaact gttccgcgtg cgctcagccg cattcaccgc atcacaaaat
     1021 tcactttaaa aagggcggac atcagccagc aatgaaactg atgccgccaa aggtaccaat
     1081 caacatggag tgttgtggcg tggttgtcac ttaagcgtat ggtcaacctg acaacccggt
     1141 gtcctcaacg gggaaggaat aactccgcca tacttaccgc cgcgtcattt cgcgttgtgt
     1201 gcctgctttt aactacgtca ggcgaggtgg ttcctgttat tccccaacaa caagaaattt
     1261 gtataatctg aataccccaa caataagaag agtgagcaag tgatgtatta gcgatgtcag
     1321 ctctcaaaga gactgcatca atgttaaaat tattcaatga agcaaaaact caggctgagg
     1381 ttgatcgcgc tgtgtctgaa ataaactcta agttgtcaag cattcagctt gaatatttgt
     1441 catctcattg atgagctgac aatctctaaa agcaatgagg ctgcgctcaa agcaaaactc
     1501 acggagatag aaaagtttaa gttccaaagt gaagactgcg aacctcgcta attgattact
     1561 ggaacgattg tgtatgccag acagtgcaat actagctctg gcaagattac catgtatttt
     1621 tgtccagaat gttttgcgaa gagagcaata tctatattgc aacctttccc tatcgaggaa
     1681 ggcgataaat atacgaaaac tcaatgcccc tcctgtaaaa atgagtatct catggactac
     1741 aatttcaata atgatttttg agtcacggta ttcagatttt taaagaacat gccggatact
     1801 cacccgtgtc cggcgcacgc actccacctc acccgtggag aactccttaa ttaccaacct
     1861 tggcttcgtt ggttagccat taacgctggt atgtaatcat tctggcaatg cttaatttga
     1921 agtggcacac tgaatttggc cacctgaaca gaggtgatat gctcacctca gaacaa
//
LOCUS       DAJTSF010000264         1964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000264 DAJTSF010000000
VERSION     DAJTSF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.273"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="OO335_004267"
     CDS             complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="OO335_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6004389.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          622 a          332 c          311 g          699 t
ORIGIN      
        1 tcaccacatg caatgtgttg ttgcagggga acggtctgca ccatccccta tatcgatcaa
       61 tataacaaaa atgacaacgt cagattcagg gcacaaccta aatatatttt aggtcacctc
      121 ccaaatcgtt tgcctgatac cgctccattt tttaataaaa aatcaattgt ttttgaatct
      181 ttgcttttca ttgcattgtc aagcatagtg tccccactat ttgctttttg aaatcttttg
      241 tttacatcaa ccaatccatt actaataaaa tattctagca ctttatagtt ggcactatct
      301 tcactaagta aatactcaac atcatacagc tcacaataca tattgttgtt cattttctcc
      361 atctctgcga tatcttgttt tgtaaaatta aatttagata gtaaataaag tgccatatca
      421 gattttgcat tgcctagaga ataagcaaga gcttgatgag ccattttttt atcagtcacc
      481 ttattgttaa tttcttcata attggatgct ttaatggcat cctccaaaga tatatctctg
      541 agtcttatct ttttgaagtt atctgtgctt agcattcttg gtatatgata ctcaagctga
      601 aacacaaagt ttataattct atccagacta acaggatcaa tgtttttatt ataacaaaac
      661 tctcggaatc tttgatcttt actctttctt ataaaatcaa taagatgcaa cccaattcca
      721 agcctcatat ctttagtatt aaatattggc acatcagttt ttccctgatt accatgcaca
      781 tacctgctaa cttcatccac aacctcttta aagttatcta agaaaaatga ttggtgctca
      841 tggtaaaaaa caggtggaat agcattatca aagtggtcgg ttaatgtcat atatccatat
      901 ggagagtcat gatcaatgat atacgcatta gcaccaaaat ccacagtatg atgtgtttta
      961 tttagtggga gtgtttcttg atgattggaa atttcaaccc caaaaaaaac aaaatcgtgg
     1021 ttagctattt cttcaatatc ctcagagaat gtgtttttat atgtttgtat attctcagac
     1081 ttaattttat caaaggactt aatattaaga gtattatttg acaggttagc gttactttga
     1141 tgtctgaagt taaaatcaga attaataata atagagagca agtgacgttc atccgaagaa
     1201 agaggatgag ttttttgtat tatttcagaa agatagtgtc tggacacctt atcgaggtgc
     1261 tcaagtgacc gaaatgatga ccgtgctcca gatggcgctt caatagaaag atttaatgat
     1321 ttgaaactgg tcccttgaga gaacatttta ctataacttt gcgctgctat agacttcctt
     1381 aaaaagcttt tcacagtatc agcacagtga tctaaatgtt tttcacttat gccagaagag
     1441 gatgagtttg ttacgcatga tatgtttttt tgtaatgctt ttgtatcttt gttcggattt
     1501 atttttccat caacaagatc aatattgttc tgaacattaa tatgatttac tgcttcaggt
     1561 attttcataa aacatccccc attatgtgcg tttttatctg cagttattta gaataatatt
     1621 atctaactta atattttctt gtatgtatta taacgaacat attttaatat gttgtacgat
     1681 tttcactctg aaatcccctg agtttaatca cacttccttt actgaaagga attattgcaa
     1741 cagtattatt tgtttttgga tatatatctt gcgccttgtt atgtagttaa tgctcagaat
     1801 atgatagata aaatagagac gcaaaacagg gcatgctcat aacatgatga taacataaaa
     1861 aattagtaac aataaatgaa agaatcatgg gcgctcaaca aaaagagggt aatgctgcca
     1921 acttactgat ttagtgtatg atggtgattt taaggtgctt gcgt
//
LOCUS       DAJTSF010000265         1959 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000265 DAJTSF010000000
VERSION     DAJTSF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.274"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            230..499
                     /locus_tag="OO335_004268"
                     /pseudo
     CDS             230..499
                     /locus_tag="OO335_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            496..722
                     /locus_tag="OO335_004269"
                     /pseudo
     CDS             496..722
                     /locus_tag="OO335_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            751..1341
                     /gene="ospG"
                     /locus_tag="OO335_004270"
     CDS             751..1341
                     /gene="ospG"
                     /locus_tag="OO335_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR6004390.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1680..1959
                     /locus_tag="OO335_004271"
                     /pseudo
     CDS             1680..1959
                     /locus_tag="OO335_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          556 a          430 c          459 g          514 t
ORIGIN      
        1 ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag tgataggaca
       61 ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta ctcctctgcc
      121 atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct ggtgacgata
      181 tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa tggcacaggt
      241 taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg ctgcaaaaag
      301 catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg ttgaacggca
      361 gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg aatgcggtga
      421 tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg aaatgagcag
      481 gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca ggacaggaag
      541 cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg gacgaacgga
      601 ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac ggtcgtgtgg
      661 aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa aatctcttgt
      721 gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat tattcaaaca
      781 ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc cattctcggt
      841 aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga ttcatctgct
      901 ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga aatggcttgg
      961 caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt tatacagtat
     1021 ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag tgacattgat
     1081 acagctgata tccctgataa tatagagagc ctttatctac agttgatatg taaattgaat
     1141 gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga taaagaaagt
     1201 gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc tgcaaccaaa
     1261 aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt ttattcgtta
     1321 ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg ctcttctatg
     1381 agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta tgcaccgcca
     1441 gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa ccacccgaaa
     1501 aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca acaaggcgaa
     1561 tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt aaaccgtctg
     1621 gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga cactatcgta
     1681 tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa catttaccac
     1741 gggttgtggc cggatgtcgc ctgggcgtac caaaatcaac tgtctgtggt atgttcgtgc
     1801 gcttccgcaa cgctggcctt tcatggcctc tgcccgcagg tatgtcggag caggaacttg
     1861 atgcccttct ttacggaagt gcctccacag tacctgtcgt acttactgaa agcaccgtta
     1921 tgcccaaact cccggtggta aaaaaacgtc cccggcggc
//
LOCUS       DAJTSF010000266         1913 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000266 DAJTSF010000000
VERSION     DAJTSF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.275"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(53..1702)
                     /locus_tag="OO335_004272"
     CDS             complement(53..1702)
                     /locus_tag="OO335_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6004391.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          506 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAJTSF010000267         1861 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000267 DAJTSF010000000
VERSION     DAJTSF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.276"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..345)
                     /locus_tag="OO335_004273"
                     /pseudo
     CDS             complement(1..345)
                     /locus_tag="OO335_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(345..1265)
                     /locus_tag="OO335_004274"
     CDS             complement(345..1265)
                     /locus_tag="OO335_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6004392.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1403..>1861
                     /gene="pgrR"
                     /locus_tag="OO335_004275"
     CDS             1403..>1861
                     /gene="pgrR"
                     /locus_tag="OO335_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR6004393.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVLRCTGNSG"
BASE COUNT          466 a          451 c          433 g          511 t
ORIGIN      
        1 acacacaccc agcacgccaa tgcgttcagc atcaacatat ggcagagtta ctgcataatc
       61 gataacacgg ctaatatcct ctacgcgctg tgtcggatct tctatccaac gaggctaccc
      121 accagattct ccctggaaac tggcgtcata agcgagaacg acatagcctt tttcagccaa
      181 tgccttgcca tagatattgc cagacgtctg ctccttacaa ctgccaaaag gatggacgct
      241 gataatcgcc ggatactgtt ttgactcatc aaaatttggt ggaaactgaa tattggcagc
      301 aatatgccag gccatatcac tattgcggat ttgaatatgt ttcattacag agtttcctca
      361 aagaacggag ctaatactga aattgcttca gccacatagg ctttgccgtc ataaagatcc
      421 atatggtttg cgccttcaac aatgtgatag cgtttatcct ggcttgatgc ccgatcgtac
      481 aggtcgtcac tcatccattt gctccctgcc tggctgcccg ccacaatctg catcggctga
      541 gtcaggtaca cttccgccat atggtaagca tcataggtaa taatctggtt aaggctgcgc
      601 aaagtagcgt aacccggtgc tgttggatac tgcgcacgag gtgtgtggta atactcccag
      661 gcctgacgca gttcttcgtt cggcgcatcg gcctccttca ttggagccag tggcataatg
      721 gcgtattctt cgctgctaat atcgctggtt cgggcgtttg agccagcttc aatgtgtggc
      781 agagcatcaa tagatttcac attgttttcc caaccattgc ggaacatcga accaatattg
      841 accgcactaa cggtaccgat ggccttgatg cggcgatcct gaattgcagc attggctgta
      901 tatcctgcac cggcacaaat tcccatcgca ccaattcggg tattgtcgac ataagaaagc
      961 gttgtcaggt aatcaatcac ggcactgacg tcttcagtac gaatgtatgg gttttctaac
     1021 tgacgcggct cgccgccact ttcaccttga taagatgcgt cataagcaat agtgacaaat
     1081 cccttttccg ccagcttttc ggcataggtt ccggccgttt gctctttaac gcccccacct
     1141 gggtgagata ccacaattgc cggatactga cgggtttcat caaattttgg agggaaatag
     1201 atcactgcag acaaagagat ggttggatta ttgctgttag tgaagctgac tttattattc
     1261 atcattcagt tcctcatgga gttgtcggtt cgttttgacg gttggtgata tcactataga
     1321 tattgatcat taagttgatt agaccctaaa tcatgattag acctatcatt taaatgatta
     1381 atagattgta tggagatact gaatgaagcg tgaagaaatc gctgatctga tggcgtttgt
     1441 cgtcgttgca gaggagcgta gcttcactcg cgcagcagcc cgcctgagca tggtgcagtc
     1501 agctttaagc cagatagtgc gccgtataga agaacgattg ggattgcggc ttctgacgcg
     1561 aaccacgcgc agcgttgtac ccactgaagc gggcgagcat cttttgtctg ttcttggccc
     1621 tatattgcat gacatagatt ctgccctgac atccctgagc gatctgcaga accgcccatc
     1681 cgggacaata cgtattacta ctgtagaaca tgcagcaaaa acgatattgt taccagccat
     1741 gcgcacattc ctgaaatcgc atcctgaaat tgatattcag cttaccattg attatggttt
     1801 gaccgatgtc gtttctgaac gttttgatgc aggcgtcctg aggtgtactg gcaatagcgg
     1861 a
//
LOCUS       DAJTSF010000268         1831 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000268 DAJTSF010000000
VERSION     DAJTSF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.277"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..179)
                     /locus_tag="OO335_004276"
     CDS             complement(<1..179)
                     /locus_tag="OO335_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6004394.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITH"
     gene            complement(320..874)
                     /locus_tag="OO335_004277"
     CDS             complement(320..874)
                     /locus_tag="OO335_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6004395.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(871..1161)
                     /locus_tag="OO335_004278"
     CDS             complement(871..1161)
                     /locus_tag="OO335_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6004396.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1161..1760)
                     /locus_tag="OO335_004279"
     CDS             complement(1161..1760)
                     /locus_tag="OO335_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6004397.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          496 c          420 g          470 t
ORIGIN      
        1 ttatgggtga tcggggagag agtttcaaca gagatttgag tggtcataac gataactccg
       61 tacatttgga aattatcgcc accgacgacg ccaatcgaac tggtggcgaa ctgtgcaggg
      121 ttggcgtaac cgggtacgga aaccggcgag cctttcggct cccccacaca gcccgccata
      181 aatcgcgaat gtgactgtgc aaacgatatg aaaaaagacg cgggcgcgtc tcatatcgct
      241 ccgtaaacat ccgggacgcc aatcccgacg ccagattttg ctggcgcgtg aggaatatag
      301 ccccggataa cagattgagt caacaaacgg tttttagatc cccggaagag aatgcatcac
      361 gcatcggtag atagagcata tattcagcaa gatttagcca tacatctacc cgactgcagc
      421 acgtccggaa acaccactca gggtgtgcgt cattcagcaa ttcagccatt ttacgcttag
      481 tcatcccccg cccttcatac cgttgccgga ggacactaat caatccagga tgctctgcca
      541 gcacctcact tatgactcta tcaatacata acgcctctgc atcagtacaa tgcgccagcc
      601 aggtcttttg cttgccattg atcatctctc gcaaaaacgc ttccagctca ggtttctcta
      661 ttcccgcttt tttcatcctg cgcagggctt cattgacggc tgttttcgtc aattttttgg
      721 atgccagcaa ctggttaaac atatttcccg tcttaccgcc gccaatatac gaccagcgcc
      781 cccacatgcg cagttttccc tgaatccaga cactttccag cgtggtgaga cgaaggtgtt
      841 ccccgctttt gcctgtattt gttgggtaaa tcataaataa ccttcctttc tccagatttc
      901 ttgtgtgcga aaaacacctt ctgcatgcat caggcgtaat tcttctttgg tgtaatcgct
      961 ggtttttacc cgcccgtcga ttaaatcgtg gcatgagcta caggcaatcg ctgcctgcat
     1021 atcgtgtggt tttgtcgctg ttccgcacgt ccccgccagc ctgtaatgcg ccagcacaga
     1081 agtttcggga ttgtgattgc agtagccagg aattctgacg gtgcacatct gcccccgcgc
     1141 cgctttacgt aaatccacca ttacgcaaac tccagtaact gcgcggccac attttcgact
     1201 tcctccggag aggaaaattt acggaacagg atccagttcc acagcacatt cagtacagat
     1261 ttataaacct gctgaaactc ggtttcgtcc atgttcgcaa atgcgataga ttttgccctg
     1321 cgcccacgac taccatcagg ataaatatgc tcggtgtaaa atccggcctg aatggttacc
     1381 cactcgcgga aagcgtcaaa cgactttagc aatgccgtat cccgggttct gcatgtcgca
     1441 actgtattaa ggtattgctc tgcggcatca ctcagggctg gagtgtgttc ccgacctact
     1501 gattcgcaca ggtaatcaac gaagcctgat accagttttc gttcgcgagg cgtgatcgcc
     1561 ccaccgaccg gagtccagta atcaaatccc agttgcagga gtttgaaaaa acgcttgtgg
     1621 aatgcgtagt tacgcacacg cttaaagtct gcgtgtatcc actcacctat tttgatttga
     1681 tgcaaaaaat cgcaactctc cggcgtcgcc gggagaagta atccggaaga ggtttgtttg
     1741 accagttgta tatgcgccat cgtagttctc cgctggcgca gtagaatggg tgttcagccc
     1801 gttatgtagt ataccagaat taatgccaat a
//
LOCUS       DAJTSF010000269         1759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000269 DAJTSF010000000
VERSION     DAJTSF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.278"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..263)
                     /locus_tag="OO335_004280"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="OO335_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO335_004281"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO335_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR6004398.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1175..1645)
                     /locus_tag="OO335_004282"
                     /pseudo
     CDS             complement(1175..1645)
                     /locus_tag="OO335_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          502 a          433 c          319 g          505 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttacttttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagta ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcgt gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg actaacagtt
     1201 cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacga ataccagagc cagtgttgtt gcagccttgc tgccaacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctgg
     1441 cggtatcacg gccccctgcg ccgttatgtg ccatttcaac gatctgctcc ttaacgccgg
     1501 gttttctggc ttcataagag taagtgagct ggaaaacacg tttacatgag cgacaacgga
     1561 aacgctcatg ccgggaacaa ctacggccat gtcgataaac ttcatcggaa tgacagcgcg
     1621 gacaatgaac agtaacggta gccatggaaa accttaaata ccagtagatt accagaaact
     1681 tctgtcgatt gaagacatca cccgaagttt gaattcattc gaatactttt tacgaggttc
     1741 ttttcgccag tcctgtaat
//
LOCUS       DAJTSF010000270         1749 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000270 DAJTSF010000000
VERSION     DAJTSF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.279"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            455..1303
                     /locus_tag="OO335_004283"
     CDS             455..1303
                     /locus_tag="OO335_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6004399.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1320..1601
                     /locus_tag="OO335_004284"
                     /pseudo
     CDS             1320..1601
                     /locus_tag="OO335_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          500 a          399 c          411 g          439 t
ORIGIN      
        1 cagcattata tagttctact ctggcgagat acgttggcat attcatttcc ttactggttg
       61 tgtgagaact tcagtaaaga taccaccaaa gcccggaagt ggtgaaataa aaccgggcac
      121 aacacgaagg cgcatttccg gtatccataa agagtcggtc ttgtctgtga aatttaaatg
      181 gtgggagtgc gcttccggtt gtaaataacg acattgctgt gtgaagtacc agttggcggc
      241 atcggtttaa ttgctggctg atgtccgccc tttttaaagt gaattttgtg atgcggtgaa
      301 tgcggctatg cgcacgcggc acagttaaaa gtcatgttag tccttattgg tttgggtggg
      361 aaagccaact gtaattgtta actggttaac gtcacctgga ggcaccaggc accgcatcaa
      421 caaagttcac ttcggtgatg aaaggaaaga gaaaatgttg aatgtagcta ttgaaaacca
      481 gaacgggtgg aattatagtg cacctgcacc tcataaaacg ggtgccggga ttgctacccc
      541 gacgatgact actgcgcata accgcgctca ggcggttttt ttatgcgtca agcacagcca
      601 cattcagatt atggtggggc gtgcagggca gccgcaaggc tggccggttt cggtagtcac
      661 cggttgtagc aaccctgtgc gtctcaccac ccatgagatt gctacctctg gtggtgagtc
      721 atttaaactg actatcgagg ctgccatcat ggctactatc cttacccttt ctcaccctga
      781 cgcaaccatc gaaaatggtc gcgctgtcac tacgtctgtt gcggttgcgg agttcttccg
      841 gaaaatgcac aaaaacgtta ttcagaagat cgaaactctc gaatgctcgc cggagtttaa
      901 ccggcttaat tttaagcccg tcacctatac cgacgccaaa ggcgaaaaac gcccaatgta
      961 ccaaatcacc aaaaacggct tcgttttcct ggtgatgggc ttcactggca aaaaagccgc
     1021 cgcattcaag gaagcctaca ttgctgagtt cgaccgcatg gaggccgaac tgcgccagaa
     1081 taacacccca cctgcagaca agatgattcc gggtgatggt cgcactctgg ttgttcactt
     1141 cgacaaattc ggcaatgtcg aattcaccga aaccgttcct gatggcgcac tggtctgtac
     1201 cctggagact ttccggtttt atctggaaaa acagggatgg actcttgtta accggggcgc
     1261 aattaaaaat atgactgtgg aacaattact ttctttaaaa taaaaatgga gctacggata
     1321 tgaaccctat gcttgattat tgttttacct ctgcacctca gcagaccata acacacaccg
     1381 gaatcaaaaa tttgttatgg ataatcaaaa aaaacctcca gtagaaaggt gatcccgatt
     1441 taattccctg tgcttatggc ctgtgtgaca gacaagtaag ccctgataag gcttacactg
     1501 ctgaacttat ttacatgcac ggaaccatga tgaggaaaaa gaaacgcaaa tattgtagtg
     1561 aaatctgcgc cgaaaaagac cagatggcac acgaacttta attaactgac tattcgaaac
     1621 tgaatttatg ccagcaatgg cagggattcg ctcaacctta attaaggaga aaaacatgaa
     1681 caccaattat gaagccactg ttgcaactac tgataacatt gttcacgagg tttacctgga
     1741 aggaaagca
//
LOCUS       DAJTSF010000271         1705 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000271 DAJTSF010000000
VERSION     DAJTSF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.280"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            84..992
                     /locus_tag="OO335_004285"
     CDS             84..992
                     /locus_tag="OO335_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004400.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
     gene            1231..1290
                     /locus_tag="OO335_004286"
     CDS             1231..1290
                     /locus_tag="OO335_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6004401.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1607..1665
                     /locus_tag="OO335_004287"
                     /pseudo
     CDS             1607..1665
                     /locus_tag="OO335_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
BASE COUNT          488 a          404 c          458 g          355 t
ORIGIN      
        1 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca
       61 ttaccgaaca acaagaagcg tttatgacgc ttgagcagga gcagcaggtt aaaacccgta
      121 ctgccgaaca gaatgcacgt attgcggctt ttgaagctga acgtcgtcgt gaagcagagc
      181 agacgcgaat tctggctgaa cgacaaattc aggaaacgga gatcgagcgc gagcaggccg
      241 tccgctcaag aaaggttgag gctgaacgtg aagttcgtat taaagagatc gaacagcagc
      301 aggtcaccga aatcgccaac cagacgaaat cgatcgctat tgccgccaaa tcggaacaac
      361 agtcccaggc agaagcgcgt gctaaccttg cactcgcaga agcagtaagt gcgcaacaaa
      421 acgtagaaac cactcgccag accgcagaag ccgatcgtgc taaacaagtt gccctaatcg
      481 ctgccgcgca ggatgcagaa accaaagcgg ttgaactgac cgtgcgggcg aaagcagaaa
      541 aagaagccgc agagatgcag gcggcggcta tcgttgagtt agccgaagct acacataaaa
      601 agggtctggc ggaagcagaa gcacaacgtg cgctgaacga tgctatcaac gtactttctg
      661 atgaacaaac cagccttaaa ttcaaactgg ccttgttgca ggcgctacct gcggtaatag
      721 aaaaatccgt tgagccgatg aaatctattg acggtatcaa gattattcag gtcgatggtc
      781 tgaatagtgg cagcgctgcg ggtgatgcaa acacgggtaa tgtggggggc ggaaacctag
      841 cggaacaagc attatcagcc gctctctctt accgcacaca ggctccgctg attgactcct
      901 tgctcaatga aattggcgtt tcaggcggtt cactggcggc attgacttca cccttaatct
      961 caacaactcc cgtcgccgaa aacgtaaaat aaattttcag ctatgaacgt ccctggcaca
     1021 aaaagccggg gattttttat atctgcgttc cgctaaaaac tgcaaatgat caggtcgttg
     1081 caacgttttg cgtgaccgct cggggaaggc aaaactgcct ctgggaaagc gttgcgcgag
     1141 gtccggcgct catcaacaat cggggagtca gcaaagggct gaaacgggaa agcccctccc
     1201 gaggaagggg ccttacagaa ggaaagggtt atgatgaagc tcgtcatcat actgattgtg
     1261 ttgttactcg taagtttcgc agcttattaa cagccaatca gaggggggag aaatcctccc
     1321 tcacccttgt tcctttactt taggttgaaa aaaaatcagc gtcaagaagg tttcattaac
     1381 acatatccct ggcccaaaaa gccggggatt ttttatatct gcgttccgct aaaaggtgca
     1441 aatgctcagg acgttgcagc gttttgcgtg accgctcggg gaaggcaaaa ctgcttcagg
     1501 gaaagcgttg cgcggggtcc ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa
     1561 cgggaaagcc cctcccgagg aaggggcctt atagaaggaa agggttatga tgaagttcgt
     1621 catcatactg gttggttgtt actcgtaagt ttcgcagctt attaacagcc aatcagaggg
     1681 gggagaaatc ctccctcacc cttgt
//
LOCUS       DAJTSF010000272         1679 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000272 DAJTSF010000000
VERSION     DAJTSF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.281"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(72..539)
                     /locus_tag="OO335_004288"
     CDS             complement(72..539)
                     /locus_tag="OO335_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6004402.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(605..844)
                     /locus_tag="OO335_004289"
     CDS             complement(605..844)
                     /locus_tag="OO335_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR6004403.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(979..1584)
                     /locus_tag="OO335_004290"
     CDS             complement(979..1584)
                     /locus_tag="OO335_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6004404.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          541 a          370 c          294 g          474 t
ORIGIN      
        1 agacctactg ctatcacaat tactgagaga aaaaaaagca tccgatgatt catttatgct
       61 gtcctaaaaa attaacaaaa caatgggaaa acaggatatg taagcagaaa agccaatgaa
      121 attggtacag cataaggcac accgttttca cgaattgatt ttcgaaaaaa aagaagacct
      181 gccagaacaa ccagagcgcc gcaaaaggtc attgcgacga gaaaattagc cagttcatgt
      241 tcatcaatag ctaacgaaag aacagttaac agcttgacat caccgccgcc aattatccct
      301 gcggaaaaaa gaaagaagcc cacgagcaaa acgctcccgg ctgcaaccag attgggtttt
      361 tgatgctgta aaatgagtgc caaaaaaata agcacggtta acaccagtaa ataacgatgg
      421 ctaataattc tgagtaatat atccccccat gaaataaaaa taagaaagag cgcaacaaat
      481 aaaagatcga ctcttagtaa ccatattgct gccattgaaa gactccatgt accatacaag
      541 gttgctgcca gacatattcc tttacgtctg acagcctcta gagagttttt ttatttttta
      601 taagttacgg attactcgtt ccggaaatcg ttacctgttg aattgctgca gcgatagcgt
      661 cgaatgcatc tttaattgcg cccaggaagc ctgagttctg atcgccaaag atgaaagcaa
      721 gcagagttgc catagcaaca ccaatcaatg catattcgat tgcagtaaca ccacgttcat
      781 ccttcgcgaa acgcgcaaag cactgtttag ttttaccata gtacttagta ataaaagtat
      841 tcatgttttc ctcgtatatt attatttaaa ttcaaaccac cagcttcatc tgcgtgtcac
      901 cggtatccca tatttttggg atgtggctat aattatctat tcccgctatt tagtcttcaa
      961 aaaaaattac atttaatatc atttatcttg attaagaaat agtaagacta aaaaaacaag
     1021 cgtcgtcagc cagcacactg ctaacacggt aagaacgcta ataaaaacac tccactgctt
     1081 cgttctatac cattcaatat ttttgtcagt atgttttatt gggagcagct cttcagtaga
     1141 gaattcctgg ctgttatcat tctcaggaat gtttgcatca gtgtttactt ccctggcctc
     1201 gctatcgttg gtatagctac tactgtcttc atcactgtca cgctcattct cacagacatg
     1261 tttctcgacc tttcccagat atctgacgcc ttttcgcgga atggttttaa ttagagaact
     1321 cgacaaacct gcgacgtcta atgcctttct aagcatgtaa atctgcccat aataactgct
     1381 gtcgcttacc gaacctttct gctcccgcca aacctcattg ataatatgtt gcttatcgga
     1441 ttctccctgt agtaaaagga gtaaaaatcg atagccgttt tcagacagat tgattacatt
     1501 gcctgtactg gtggtgatac gtcgagacgc tgggtaaaac ttaactccct gccctaaaga
     1561 aaaataaaaa cggtcatcgc tcataagtac cgacccctgt tttcttcttc acatgactac
     1621 tcattacaac tttgtatgaa gtaagatgca aaatcatatt gtttacatta agttaagca
//
LOCUS       DAJTSF010000273         1677 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000273 DAJTSF010000000
VERSION     DAJTSF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.282"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..143
                     /locus_tag="OO335_004291"
     CDS             <1..143
                     /locus_tag="OO335_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6004405.1"
                     /translation="AEVELATLTWVDWYNNRRLPERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            127..486
                     /locus_tag="OO335_004292"
     CDS             127..486
                     /locus_tag="OO335_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004406.1"
                     /translation="MICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQ
                     ENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLEN
                     VNITHWAVMPKAPEAGA"
     gene            486..791
                     /locus_tag="OO335_004293"
     CDS             486..791
                     /locus_tag="OO335_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004407.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            791..1141
                     /locus_tag="OO335_004294"
     CDS             791..1141
                     /locus_tag="OO335_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6004408.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYGTPVSHCRTVKDLNSLL
                     EQRTKGRRQNSMTS"
BASE COUNT          549 a          367 c          413 g          348 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 cggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcagaaag taaatgaaac agatgctgaa tacctggtgc gtaaattcga tgagcttgaa
      241 gctaaatgtg cagcacagga aaacaaagta ataccagtgt caactgaact gccaccagca
      301 aatgaaagtg ttttgttatt cgatgctaac ggagaaggct ggctaattgg ctggcgttct
      361 ctctggtaca cctggggaca aaaagaaacc ggagaatggc agtggacatt tcaggtcggg
      421 gaccttgaaa acgtcaatat cactcactgg gcagtaatgc caaaagcacc ggaggctgga
      481 gcataatgac cacttttacc gacaaagaac tgattaaaga aattaaagag cgtatcagca
      541 gccttgacgt gcgagacgat attgagcgcc gtgcttatga aatcgcactc ctatctctgg
      601 aagtagaacc agatgaacgc gaagcttatg aattattcat ggaaaagcgt tttggtgact
      661 tagtagatcg tcggagagca aaaaacggcg ataacgaata catggcatgg gatatgactc
      721 tcggttggat cgtctggcag caacgagctg gtatccattt ctcaacaatg tcacagcaag
      781 aggtgaaata atggagccat acagcctcac actcgatgag gcctgtcatt ttctcaagat
      841 atccagaccg actgccatta actggatacg cacagggcgt cttcaggcaa cacgcaaaga
      901 tcccactaag aataaatctc cttacctcac aacacgacaa gcctgcattg cggctcttca
      961 gtctccgctg catactgtcc aggtgagcgc gggtgatggc ataacagagg aaagaaaatg
     1021 tcactcttcc gcagaggtga aatatggtac gccagtttca cattgccgaa cggtaaaaga
     1081 tttaaacagt ctcttggaac aaaggacaaa aggcaggcga cagaactcca tgacaagcta
     1141 aaggctgaag catggcgggt cagcaaactt ggtgaaatac ctgatataac gttcgaggaa
     1201 gcgtgtgtca ggtggcttga agagaaagca cataaaaaat cactggacga tgacaaaagc
     1261 cggatcggat tctggcttca acatttcgca ggaatacaac taagagacat tactgaatca
     1321 aaaatttatt cagcaatgca gaaaatgacg aaccggcgtc atgaggaaaa ctggaaactc
     1381 agggcagaat catgcagaaa aaaagggaaa cctgttccag aatacacgcc aaaaccagcg
     1441 tccgttgcaa cgaaggctac gcatctttca tttataaagg ccctactaag agccgcagag
     1501 cgtgaatgga aaatgctgga taaggcacca attattaaag tgcctcaacc aaagaataaa
     1561 cggatccgct ggctggagcc ccatgaagca caaaggctga ttgatgaatg tccggagcca
     1621 ttaaagtctg ttgttgaatt tgcactggca acaggcttaa gacgctcgaa catcatc
//
LOCUS       DAJTSF010000274         1644 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000274 DAJTSF010000000
VERSION     DAJTSF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.283"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..430
                     /locus_tag="OO335_004295"
     CDS             <1..430
                     /locus_tag="OO335_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004409.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
     gene            550..723
                     /locus_tag="OO335_004296"
     CDS             550..723
                     /locus_tag="OO335_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR6004410.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            823..1644
                     /locus_tag="OO335_004297"
     CDS             823..1644
                     /locus_tag="OO335_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR6004411.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
BASE COUNT          427 a          416 c          420 g          381 t
ORIGIN      
        1 aagtcctgac acaaacgccg ccactgtaaa tcagacactt aaatgcgact caaaagcccg
       61 ggctgaccag cctggtgcat gtccaacaac gtacgagtta tacgaaggtg acgctaccta
      121 cagagctgct atcgacaaag cattaaaccc tgttgggtta aacggaatgt ttggggaaga
      181 cggctatatg gatggtcctg atggtggtgc gtatcctgta aacattaacg gaacgacctg
      241 ggtagaaggt ggcggttgta aagcccacgc ctgcggctgg gactatatcg taacactcta
      301 taacccaaaa acgcataaag tcgttggtta ctattacaac atcgaccctg gttacctgat
      361 ttggtttggt gaaactggcg ttcacgaatt cgcgtacctg gtcagggact acgtaaacaa
      421 aacaaattaa aggcgtaagt tatgcggttc atcttcgcgt tattaattta ttgatatcaa
      481 aagtaaatac ggctctgcca tgatgacagg gacttatatt ttcagggaac caaatacagg
      541 tctggttgta tgtcacgttt attgcccgaa gtccctttct cacgcgagca ggctgttgtc
      601 atcacaacag cttaccgcaa tgtgtttatc gacgatgacc agggaactca tttccgtcgg
      661 gttatccgca acgcagagac tgacgccgga aggttcgcca gtgacggtct tctcagacaa
      721 taaacctcat taatttcgtc catcaggccg cgtcttctcc gggagacgtg gctttttcat
      781 ttattacacc agtaaatctt aaccaccgat aaggagcaaa ttatgcgact agccagtcgt
      841 tttggttatg ctgcaaacca gatacgccgt gaccgtccgc tgacacacga agaactgatg
      901 cactatgttc ccagtatttt cggtgaagac cggcatactt cccgcagtaa acgctatgcg
      961 tacactccca ccatcaccgt actggaaagc ctgcagcggg aaggctttca gccattcttc
     1021 gcctgccaga cacgtgtgcg tgacccgggt cgccgggaat acacaaaaca catgctgcgt
     1081 ctgcggcggg ccggacagat aaccggtcag catgtccctg aaattattct gctcaactct
     1141 catgacggtt catccagcta ccagatgtta cccggatatt ttcgtgccat ttgtaccaat
     1201 ggactggtct gcggtcagtc tctgggagaa ttgcgtgttc cacaccgggg aaatgtggtg
     1261 gagaaagtta ttgaaggggc ttacgaggtg gtgggcgtgt ttgaccggat agaggagaag
     1321 cgtgatgcca tgcagtcgct ggtcctgccg ccaccggcac gccaggcgct ggcacaggcg
     1381 gcactgactt accgttatgg tgacgaacat cagccagtca ccaccgctga cattctgaca
     1441 ccacgacgca gggaggatta cggtaaagac ctgtggagcg catatcagac catccaggag
     1501 aatatgctga aaggcgggat ttccggccgc agtgcaaaag gaaaacgtat ccacacccgt
     1561 gccattcaca acatcgacac cgatattaag ctcaaccgcg cattgtgggt aatggcagaa
     1621 acgctgctgg agagcctgcg ctga
//
LOCUS       DAJTSF010000275         1601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000275 DAJTSF010000000
VERSION     DAJTSF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.284"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(333..479)
                     /locus_tag="OO335_004298"
                     /pseudo
     CDS             complement(333..479)
                     /locus_tag="OO335_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            533..715
                     /locus_tag="OO335_004299"
     CDS             533..715
                     /locus_tag="OO335_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCR6004412.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(816..1145)
                     /locus_tag="OO335_004300"
     CDS             complement(816..1145)
                     /locus_tag="OO335_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR6004413.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1317..>1601)
                     /locus_tag="OO335_004301"
     CDS             complement(1317..>1601)
                     /locus_tag="OO335_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004414.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          435 a          368 c          377 g          421 t
ORIGIN      
        1 agcaatgtct gtcgcacaac aaccacaatg tcacaaataa aaaatgagaa taaccagttc
       61 cctgaaggcg acgtagcccg gtacaagagt aacccacgtt agcgcttggc gacggcggaa
      121 caaagctgat tcgcgacgta agaaagtggc aggctcatat gacggacctc tgtaatgcgg
      181 tatccaaccc gcgaatatca gcatgattca ccgtcggatt tactgctacg tcgccctcag
      241 ggaattaaac ctaaaaagaa aatcgtcaaa attagagatt ccggtttgac acggaaagtc
      301 atatcagcgt caacggagca ccgtattgac gcttatttat tggtgagaac tacgttccat
      361 ggcaggagtt cgtcaacacg gttggagggc cattcaggca gtatgctcag aatatggcgc
      421 agatacgctt ccggatcgat accgttcaga cggcaggtgc ctgcttttcc ggcaacaagc
      481 aggaatctca gcgctgaagc aggaattaga ggtaaaaacg ccctaccggg ccatgaatca
      541 cccggtcatt ggagtagtaa ccaaagcaga tttagccagc atggaacaaa tatctttggt
      601 gaaaagttgg ttacgggaag ctggagcgca caacgtgtta gtaaccagtg cagttaataa
      661 caatggagtt acagaacttt ttgccttgct gcatacagaa gaaggctgtt gttaatttcg
      721 attggttctc agatgagaat gatgacggtg atacacagat aagaatcaat gagcaatgag
      781 ggttgccggg caaccctcat tgaataaaac gggaattact tcgcttcgcc gtttttcatt
      841 tcgcccatag ctttcagctt tttggagata tcgcggcgtt ctttggagag ctcggcattt
      901 ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt tcatgctggc gatgatgccc
      961 tggattgctt caacgctcat ccctggcttg atgtaatcgc tcaggttgtc cagcaacagg
     1021 acgcgcttct ggttgtcacg gatctttttc tcaacgtcct gaatttcacg ttgcagtttg
     1081 ttcttacgac ggaacagacg aacaaattcc agtacgtcct ggaatgaagg cttggtagtt
     1141 tccattttta tacccctgat aatgtgagag tcggattcgt ttaagcaacc gctattcgtt
     1201 agggccaaca ttactcatag ctgccgcaga tgacaatgct tttatccctt ttcactatca
     1261 taccctttat ccttgctgaa tcgaagcagc agcaagatga ttctgaagtt caggaattat
     1321 ttgcgcaagg cccggcaaat cagattcgat agcaactcaa gctgatgtgc cgtttccatg
     1381 ttcagccaca cataaccata gattggtgtt tccacgactt tcaggtcatg gtggttatcg
     1441 agcaactgac gcagctcttc cacagcatcg ttcaattttt ccgtattggc aaaaaccggc
     1501 tgcggattac cttcgtacag cgcatgaaca aggctcagca gtatttgctg catcatgtgc
     1561 tgggtatcac aaagtttttg cgcgttcaat aacacgaaat g
//
LOCUS       DAJTSF010000276         1597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000276 DAJTSF010000000
VERSION     DAJTSF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.285"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..636)
                     /locus_tag="OO335_004302"
     CDS             complement(<1..636)
                     /locus_tag="OO335_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6004415.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGA"
     gene            complement(750..1322)
                     /locus_tag="OO335_004303"
     CDS             complement(750..1322)
                     /locus_tag="OO335_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCR6004416.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          474 a          337 c          319 g          467 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtgtttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca
     1501 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagatgaat
     1561 ttattgatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DAJTSF010000277         1580 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000277 DAJTSF010000000
VERSION     DAJTSF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.286"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="OO335_004304"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="OO335_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HCR6004417.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1580)
                     /locus_tag="OO335_004305"
     CDS             complement(1269..>1580)
                     /locus_tag="OO335_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCR6004418.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          481 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac
//
LOCUS       DAJTSF010000278         1570 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000278 DAJTSF010000000
VERSION     DAJTSF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.287"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            459..>1570
                     /locus_tag="OO335_004306"
     CDS             459..>1570
                     /locus_tag="OO335_004306"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6004419.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRP"
BASE COUNT          422 a          378 c          363 g          407 t
ORIGIN      
        1 tgttgaaaca aatgacggta gagaatgtta acactgccgt taaacaatat ctttctcatc
       61 cggtaaatac ttataccgga gtattattgc caaaataata acagccccgt caacacatcg
      121 ttggcgggga ttttagcaat attcgctatt tttatgtaat aattttataa atgcgttcaa
      181 aataataacc aagtactaat agtgatattt taatacctga tttttacgtg ataattcagg
      241 aggcacagaa agcgcataaa aataacaaca taaaacacct tgccaccacc caagaattct
      301 ctattacatt gtttttaatg aaaaaatatc attccaacaa tatctcacga taaataacat
      361 taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg ataaatccta
      421 cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag caagtaacgg
      481 atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc actatcgcag
      541 aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcatttcag attatcaatg
      601 atgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc cagacctggg
      661 acgacgaaaa cgtccacaag ttgatggatt tatccattaa caaaaactgg atcgacaaag
      721 aagaatatcc gcaatccgca gccatcgacc tgcgttgcgt aaacatggtt gccgatctgt
      781 ggcatgcgcc tgcgccgaaa aatggtcagg ccgttggcac caacaccatt ggttcttccg
      841 aggcctgtat gctcggcggg atggcgatga aatggcgttg gcgcaagcgt atggaagctg
      901 caggcaaacc aacggataaa ccaaacctgg tgtgcggtcc ggtgcaaatc tgctggcata
      961 aattcgcccg ctactgggat gtggagctgc gtgagatccc tatgcgcccc ggtcagttgt
     1021 ttatggaccc gaaacgcatg attgaagcct gcgacgaaaa taccatcggc gtggtgccga
     1081 ctttcggcgt gacctacacc ggtaactatg agttcccgca accgctgcac gatgcactgg
     1141 ataaattcca ggccgacacc ggtatcgaca tcgacatgca catcgacgcc gccagcggtg
     1201 gctttctggc accgttcgtc gccccggata tcgtctggga cttccgcctg ccgcgtgtga
     1261 aatcgatcag tgcttcaggc cataaattcg gtctggctcc gctgggctgc ggctgggtta
     1321 tctggcgtga cgaagaagcg ctgccgcagg aactggtgtt caacgttgac tacctcggtg
     1381 gtcagattgg tacttttgcc atcaacttct cccgcccggc gggtcaggtg attgcacagt
     1441 actatgaatt cctgcgcctt ggtcgtgaag gctataccaa agtacagaat gcttcctacc
     1501 aggtcgctgc ctatctggcg gatgagatcg ccaaactggg gccgtatgag ttcatctgta
     1561 ccggtcgccc
//
LOCUS       DAJTSF010000279         1561 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000279 DAJTSF010000000
VERSION     DAJTSF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.288"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            400..1149
                     /locus_tag="OO335_004307"
     CDS             400..1149
                     /locus_tag="OO335_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR6004420.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(1319..>1561)
                     /locus_tag="OO335_004308"
     CDS             complement(1319..>1561)
                     /locus_tag="OO335_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6004421.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT          426 a          332 c          316 g          487 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcactatc gccaggtaat
     1381 ttctcgccgt tttgtcgtaa cgcgtggcga tgcggcgata ttctttcagc cgcccaaagc
     1441 accgttcaac gacattacga ttgcagtacg catcacgatc aagctgtgca cgtccatccg
     1501 atgccatttt ctcatttgac tttcggggga ttactgcctt tataccgtta tttttcagct
     1561 c
//
LOCUS       DAJTSF010000280         1556 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000280 DAJTSF010000000
VERSION     DAJTSF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.289"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            624..1223
                     /locus_tag="OO335_004309"
     CDS             624..1223
                     /locus_tag="OO335_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6004422.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
BASE COUNT          408 a          373 c          353 g          422 t
ORIGIN      
        1 tggcttcacc cgtgttaata tcctggataa ctgtgtttga aggatttctg ttaataatct
       61 gctcctgcaa ctcacctgat gagtattagt ggtaatacat aatcttactc gtaaaagagt
      121 ataccttatg tcattaaaca gtcttccggc gtgttgttca atcctgtacc tctgttactt
      181 atctggtacg ttgatatgtg taatgctacg ttaattgcaa tatctggtta tggctttata
      241 ccttcagcat ttaaaatatt ttattaccgg agaaacaggg aagtggaaag actttcggga
      301 taatgtcagg ccattctgac gttaatcaca gtaccaaata acgagagggt aatggaagtt
      361 aaaacctgcg taagttcctc tgttgttcac tggaaaaagt gattgcccac acgcgttata
      421 aaggcctttc cgctgcagcc tggttaacag agcttcaggc gttatatgcg ctgtatggct
      481 gttacgtatg ctgttctttg cctggttctg atacccgtgg aagtcgttac cggaggaaac
      541 acgttcaccc tactacctga gaacatcttc ttcaaaaatt acggcaacgc ttcttcctgt
      601 gttacagaac acgaggaaga aacatgcaaa cttataacac caatcccgct tacgaaatgg
      661 ctccccagct cctggagcac tttaaccagc atctggattc attattcggg gtctattcga
      721 aactgttacc gttcagaatg gattttgctt accgaaaaaa caccctcagc tacagatgtg
      781 cctgcaggta cgccatgtgc gcggagatgc tgcaactgat taacgaggtg ggagagaaac
      841 tggtcgggta tgcctgggtg atggaatata cagaacgtaa agggcttcac attcattttg
      901 tgggctatct gaacggccag agccacagga gttcgtatct ggtatccaga ttgatgggag
      961 atatctggag gcgggttacc gagggtaatg gttactacca ctggtgccgg tttaataaaa
     1021 attatccggt aaacattaac cacgtcattc actattcaga tcacaaagcg gtgaatgatt
     1081 tacgctatgc catcagctac ctggccaaac gggaacagaa agaatgtggc atcatcctga
     1141 aatgcagcgg gcttcctgaa aaaagtaacc ggggtcgacc acgactggac agccccctgc
     1201 cggggatatg tgctctggtg taaacccctg aacgacaagt acgggctggc gctaatcttt
     1261 gctgtcggtt ccggcggcca cttcagcggc actgcgcatt ttttacggcg tggtcattcc
     1321 gtatgaacca tacggagatt taaccatgac acattcttta ccattccttc cggcaaccga
     1381 tacagcacat cccccaatct ccatctctgc ttactgactg ttttttacgt cagtgggcca
     1441 ttgtgtgaca accacagtgc tgtaacagca accagcctgc tttacccaaa ctccagtcca
     1501 gattttaact gaaccgccat tcacaccctg accacgctgc ccgtaccggg acggct
//
LOCUS       DAJTSF010000281         1555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000281 DAJTSF010000000
VERSION     DAJTSF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.290"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..255)
                     /locus_tag="OO335_004310"
     CDS             complement(<1..255)
                     /locus_tag="OO335_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6004423.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVD"
     gene            complement(320..1270)
                     /gene="virK"
                     /locus_tag="OO335_004311"
     CDS             complement(320..1270)
                     /gene="virK"
                     /locus_tag="OO335_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6004424.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSGYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            complement(1275..>1555)
                     /gene="capU"
                     /locus_tag="OO335_004312"
     CDS             complement(1275..>1555)
                     /gene="capU"
                     /locus_tag="OO335_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR6004425.1"
                     /translation="NESFGMVLAEASAFSVPVVATQIGGIPEVIQNNQTGTLLPASNK
                     HAWMCALNDFFNDPGRFYQMARLAKQDIEERFDINKTALKILTLAKQK"
BASE COUNT          473 a          342 c          292 g          448 t
ORIGIN      
        1 gtcgacaatt atttcccgtt ctgtatcact cttttctggg aaacaaagcg ataaattaat
       61 cgtcgccctg cgacgagctt ttttgctcaa tctgcccgcc caacgcccta tttttgccaa
      121 gacaggatct ctgaatgaag ggggaaacat tgaaatacct gcaatcattc ccaaaaggaa
      181 ccatgtagac cagtaaacag gggaaaggta atttttctta aattcaggaa taaactcaga
      241 tttatatttt ttcattattt agtgcgaatg cctctaccaa aattatcatt actgttttat
      301 tcagagaata catcaaacct taatttaaat tctgatgttc tgagtgtaca ggatatgcat
      361 ccaaagagcg gatagtagtt tcatggatat aatccagcaa agcgtatcgt tttcggtacc
      421 cagagcgttt tttactggct atatcgctta gcggctttcg aatcacctgg gttggtaatc
      481 tgtaccagtc accacaggtt tttcctgcta cagactccca gaaatcacta tagaccgcaa
      541 caaaggtttt gcgtttctga taccagtatc tcaattgccg gaaaacatga ctgtgctcac
      601 tgactgccag acactcagag atattgcaag ctttcatcaa cgcacaaaat gcttcaaaaa
      661 taatccgttt tgggaaaatc ccataacagg cccgggttgc gcaccgaata acatcaggtc
      721 cggtattttt aggggctccc tgtaatccgc caataaaagc aatgtgtcca ttttgagtca
      781 gaatgactga aaatgttaac cgggcaataa ctgttttatt gtaaaacaat gacaacatca
      841 actctccttc ccggtcaaaa ccactgggag aacactggat atcaaaattc tctccatctt
      901 tcccgatgaa ttggactaat gataccgatt tttgtggaaa caaaaattta gaatattttt
      961 ctggcaaatt ttgcacaaag cgataatgct caagaatata ttgtccacgg gtccaggcgc
     1021 gccccccttt atgcaaataa gggcgatgaa ttcttgctgg caacgtgggg tgtattgcca
     1081 gaatatcctc aatgcacggc aaggaacgca gttcataata atatcgaata ctgacaaccg
     1141 gatttataga acatcgcaaa atgtacatga accgaaattt tctgtgttcc cacttccccg
     1201 gtagtgaatc tgaggaaaaa ataatccttt taaggaaacc gataaatgat aagttactta
     1261 tagaaaacat attgttactt ttgcttcgct aatgtgagta tttttaacgc agttttatta
     1321 atatcaaacc gctcttctat atcctgtttt gccaggcgag ccatctgata aaaacgccca
     1381 gggtcattaa aaaaatcatt cagggcgcac atccatgcgt gcttattact tgctggtaac
     1441 aatgtcccgg tctggttgtt ctgaataacc tcagggattc caccaatctg agtggccact
     1501 acaggcacag aaaatgccga tgcttctgcc agcaccatac caaaagattc gtttt
//
LOCUS       DAJTSF010000282         1519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000282 DAJTSF010000000
VERSION     DAJTSF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.291"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(62..138)
                     /locus_tag="OO335_004313"
     tRNA            complement(62..138)
                     /locus_tag="OO335_004313"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(102..104),aa:Arg,seq:tct)
     gene            complement(140..215)
                     /locus_tag="OO335_004314"
     tRNA            complement(140..215)
                     /locus_tag="OO335_004314"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(180..182),aa:Ile,seq:cat)
     gene            complement(392..1081)
                     /locus_tag="OO335_004315"
     CDS             complement(392..1081)
                     /locus_tag="OO335_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6004426.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(1078..1437)
                     /locus_tag="OO335_004316"
     CDS             complement(1078..1437)
                     /locus_tag="OO335_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6004427.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
BASE COUNT          365 a          429 c          318 g          407 t
ORIGIN      
        1 atcgaactgg tgacctactg attacaagtc agttgctctg cctgctgagc taagtcggcg
       61 ctggcccacc accgaggact cgaacctcgg accgtcaact tagaaggttg atgctctatc
      121 cggatgagct agtggtggtt ggtggccctt gctggacttg aaccagcgac ctggcgatta
      181 tgagtcgctc gctctcacca ctgagctaaa gggccggagg cagaataata accatatgtc
      241 atcacatctg caaactcatc tgaccaccag cacgtttaac gtcctgtgcc atttttcagg
      301 cataaaaaaa ccgctcggcg gcgggttttg cttactttac catcgcgtac aaaatcggca
      361 aagtatcaga tttgcatgag atatatgcct tttaatctac ttttgcaata ctttgctttg
      421 aaaatgccgc tttttgttct gaacgtgttc tcatcacaaa caataaagcc tcactatcca
      481 gtcggtgaaa aatgtgtttc attacaaccc agtgaccagt aaatgttttg gaccagtttt
      541 tggttgttac tcccgccagt aatgccagct cctgatattc ataaccttcc ccaccaaaaa
      601 gttctgcttt taccgcctgc gccgccagcc agattaattt tttcaggcgt tcctgtgttt
      661 tctctgcaat tttcctagtg ccagaatgag ccttaaactc ttcccacgcc cagtgggtta
      721 tggtgatctg atactcccag caaatacttc cgctgtaaca ccacaacaac caggctttat
      781 agtgctcttc aagagacaga acagcccgcc gccacgatga tgtcgaaaac gcaacctgac
      841 tgaccagagg aattgacgcc cccttcgcca gtgattgttt tcccgggatt ggtggattat
      901 cccgcgttat catttttccg gtcacctcat cgcggtaccg gattttttta cgcctgtaac
      961 gccctgtatc gaacatggca ttctcctgcc aggcttcaag ctgacctttt gtcgccccac
     1021 tcaaatcagc ggtggcgata atgagctgct cacgaacaaa ctgtaaatac tggttattca
     1081 tgcacactcc agctctgtga tttttatccc caaccgacca ccaggaacag gcagtccgcg
     1141 cacaatattg gtttcatcaa actgctcgtc gtcgataagc aaccccgcat gtgtcagtgc
     1201 atccagtggt gctttcagga tattgtccag gtcacgacgc cgcttatccg gtggctctgc
     1261 aataatcttt atcgccagtc ttccggacag gcttaatttc agtcgctgct gacggacaat
     1321 aagcgccaca tcccggcgat aacgctcacc ggcttttgat acaaaatatg tgctgccacg
     1381 acgtcgccag taagtattca ccgtcggcgg ataaggtaaa ataaactcat ggcgcatcag
     1441 cgcagcacct cctgcaccag cttttcaaac tttccgactc tggtttccag ctctgccaca
     1501 cagtccacca gctcatcta
//
LOCUS       DAJTSF010000283         1498 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000283 DAJTSF010000000
VERSION     DAJTSF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.292"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1008
                     /locus_tag="OO335_004317"
     CDS             <1..1008
                     /locus_tag="OO335_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR6004428.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGCLAHARR
                     KTHDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQSLYDWI
                     QLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENALRSVAVGRKNYL
                     FFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPWNFSSV
                     K"
     gene            1197..1382
                     /locus_tag="OO335_004318"
                     /pseudo
     CDS             1197..1382
                     /locus_tag="OO335_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          409 a          352 c          431 g          306 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcctggccca cgcccgccga
      481 aaaacccatg acgaggatgt gcgccgtccg acagaaatga ctcaggaagc gctcagacgg
      541 atagcagagt tatacgacat agaagcggag atacgtggca gtccggcaga ggaacggctt
      601 gcagtcagaa aagccagaag cgtccagttg atgcagtcgt tgtacgactg gatacagttg
      661 cagaggaaaa cgctgtcgaa acatgcggag atggcgaagg cgttcgacta tatcctgaat
      721 cactggaatg cgctgaacga gttctgtcgt gacggctggg tggaaataga caacaacatc
      781 ggtgaaaacg cgttacgatc ggtggcggtt ggaagaaaaa attatctctt tttcggctca
      841 gacaagggag gagaaagtgc ggcgatcatc tacagtctgc tggtcacctg caaacagaac
      901 gaagtggagc cggaggactg gttgcgcgaa gtgatcgaga agctcaatga ctggccgtcg
      961 aaccaagtgc atgaactgct gccctggaac ttctcgtctg taaaataatc cttacgctac
     1021 gtacttctcg gggcgcttac catacggtgg tcggtttacg cttaccgcag gacaggtggt
     1081 aactgaaaaa tagtttgctg aggcgactgg aaacagaagg gcaagatcga ttccgacagc
     1141 agtgatggtc agctacctca tcgataactt tgcaacagtg cccttcttga agcatcatgc
     1201 ttcatgaaga aaaactggca cgtcatcaac gtaaacaggc gatgtatacc cgaatggcag
     1261 ccttcccggc ggtgaaaacg ttcgaagagt atgacttcac gttcgccacc ggagcaccgc
     1321 agaagcagct ccagtcgtta cgctcactca gcttcataga gcgcaatgaa aacatagttt
     1381 tacaggggac atctgacatc acaaatcccc gggtggggat ttgtgtataa gagacagccc
     1441 atggaggaag gggatatatc gcagctctga cgactcactc cttatttacc cgggaaat
//
LOCUS       DAJTSF010000284         1470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000284 DAJTSF010000000
VERSION     DAJTSF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.293"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(5..184)
                     /locus_tag="OO335_004319"
                     /pseudo
     CDS             complement(5..184)
                     /locus_tag="OO335_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            708..992
                     /locus_tag="OO335_004320"
     CDS             708..992
                     /locus_tag="OO335_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR6004429.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            992..1267
                     /locus_tag="OO335_004321"
     CDS             992..1267
                     /locus_tag="OO335_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6004430.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          401 a          368 c          361 g          340 t
ORIGIN      
        1 aaaattactc tttttcgaac tccagtgagc gttcacgact catcagtgtg aaaatgatgg
       61 caaaaacagc agacagtatc agtacgataa atgtggcccc ccatcccaga taatcgacaa
      121 caagtcccat tcccagtgtc gcaacaattg agccgaagat ataggaaaat gtgcctgaca
      181 gccctaacag aataagttta cgcgtaaatc gccctgaatc cagaagagca ttaagtatca
      241 cataaacagg ttccatacca gtcctgaccg catcaggacg ccagtaaata aaatctcgta
      301 gtccaccggc atcagagaca agagtaatcg tcgttaccgg tatttcgact ggtttgtact
      361 ggtgtgagcg gacaaaacta ctgtacgtct ctcatacacg ctcgtaagcg gccctcacgg
      421 cccgctaacg cggagatacg ccccgactgc gggtaaaccc ttgtcgggac cactccgacc
      481 gcgcacagag gcattttcat cgctgacagg ccgcatggcc tcgcagagcc ggagggggac
      541 tggtgatatt cggacgaccg taccggcttg cgccgggctt cggcggtttt acacctgtgc
      601 tacatgtaac caccgtatgc cgccttatat gcgctgactc gcattatttg ccttgttccg
      661 tctgaaagta atcactatga ttaaatatga ttaacaacta atcgggtatg caaatgaaaa
      721 acaataccgc acaagcaaca aaagtaatta ccgcgcatgt gccattacct atggctgata
      781 aagtcgacca gatggccgcc agactggaac gctcccgggg ctgggttatc aaacaggcgc
      841 tttctgcatg gcttgcccaa gaggaggagc gtaatcgcct gacgctggaa gccctggacg
      901 atgtgacatc cggacaggtt atcgaccatc aggctgtaca gtcctgggcg gacagcctca
      961 gtactgacca tctgttaccg gtgccacgct gatggaactg aagtggacca gtaaggcact
     1021 ttctgatttg gcgcggttat atgattttct ggtgctggcc agtaaacctg ctgccgccag
     1081 aacggtgcag tccctgacac aggcgccggt cattctgtta actcatccac gtatgggaga
     1141 acagttgttt cagtttgaac ccagggaggt cagacggatt tttactggcg agtacgaaat
     1201 ccgttacgaa cttactggcc agactattta tgtattgcgc ctgtggcaca cacgagagaa
     1261 caggtagatt gggtgtggag gcaactttaa catagcctgc aggtattaat tctggagcat
     1321 tgttatcagg ccagatattt tgcattccat taaataatct tgtaggagat aaaataaatt
     1381 acgatgaaat aacgaaaatt accgctcgtg actggcggca atatcgcgct ccgggctggc
     1441 aaataaccca tcagaaacgt tattgccaga
//
LOCUS       DAJTSF010000285         1454 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000285 DAJTSF010000000
VERSION     DAJTSF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.294"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            25..570
                     /locus_tag="OO335_004322"
     CDS             25..570
                     /locus_tag="OO335_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6004431.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            594..>1454
                     /locus_tag="OO335_004323"
     CDS             594..>1454
                     /locus_tag="OO335_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6004432.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNPPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKTAN
                     HLSETAAAGEKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYR
                     AKSLTFYGING"
BASE COUNT          396 a          341 c          405 g          312 t
ORIGIN      
        1 gtataacgta gcgagtattt aacaatgttg catttaaaaa atattactgc gggtaatccg
       61 aaaacagcag aacaatatca gatgacaaaa cgatattcgg tcacctggct tttttcagaa
      121 gacggaaaaa actggtatga agagctgaag aatttcgcca gcgacacaat aaaaatagct
      181 tacaccggag atggtcgcgt ggtgtgggtc ggtaaggatg tgacaggcat cgagccacgc
      241 aatgccagcg ttatcgaagt tccggatatt accgctaacc gacggattac cgcgccgggt
      301 tactggtttt accgcaatga tgaatttgtc tttgactaca gactcaaagc ggaagatgag
      361 cgtgatgccc ttctggctca ggtcagtgcc cggacagggg aatgggaaga agacctgctg
      421 ctggggttaa tcagcgacga agataaagaa aagctgaaag cctgtcgtat ttacgcgaaa
      481 tcgctgcagg cgatggattt cagcaccatc actgataaat cctcatacaa cgccattgaa
      541 tggcccgcct ctccggaagg ttcttcctga tttaatttat cgcgagaaaa acaatgtctg
      601 tagtgatatc aggtgcgctg actgatggcg caggtatccc catgtccgga taccatatta
      661 ttctgaaatc ccgggtaaac cccccggaag tggtgatgaa cactgttgct gatgtgatga
      721 caggaaacga tggtgaatac tgtttccatg cgcggactgg aaaatatggt gtgtatctga
      781 aacaggactg gcgcaacgag tacaacgttg gcgacattgc tgtatatgag gactcaaagc
      841 ccggcacgct gaatgacttt ctgattgctc ctgatgaggg cgacctgaaa ccggatgtcg
      901 tcaaacgctt tgaggaaatg gtggcgcagg cgcagcagag cgccggggcc gcagccggaa
      961 acgcacagca gacggcgcag gatgtggcgg cagccgcagg ttatgcccgc gcagcagagc
     1021 aggccaaaaa tgacattgat gctgcgctga ccggcactct gaaaacggct aaccatctgt
     1081 ctgaaaccgc agcagcaggc gaaaaggcac aacagaagtc ccgggataat ctggggctga
     1141 aaagtgcggc cacgatggaa gcacagagcg acatttacga ccggacaaaa ggccgtctgg
     1201 cgatacccgg cgcattcggc tttgggtgtg cttttctgcc tgaagatgtt atccgttttg
     1261 acactaagag tgatttcctg gcctgggtaa ggaatgcgct gccaggtgaa tattccgttg
     1321 ctggccccta cggcatcatc atacccgaca cacggtttga aggggtgctc agcatccggt
     1381 ggactgatgc acgccctgag acaacagaac cgcggtacag agccaaatcc cttacttttt
     1441 acggcattaa cggc
//
LOCUS       DAJTSF010000286         1434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000286 DAJTSF010000000
VERSION     DAJTSF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.295"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..253
                     /locus_tag="OO335_004324"
                     /pseudo
     CDS             1..253
                     /locus_tag="OO335_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..>1434
                     /locus_tag="OO335_004325"
     CDS             746..>1434
                     /locus_tag="OO335_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6004433.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          347 a          331 c          445 g          311 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgtcagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggt
//
LOCUS       DAJTSF010000287         1412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000287 DAJTSF010000000
VERSION     DAJTSF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.296"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            32..292
                     /locus_tag="OO335_004326"
     CDS             32..292
                     /locus_tag="OO335_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCR6004434.1"
                     /translation="MAWARHYQQIVREEKETELADDLEKGLPQHLFESLCIDHLQRHG
                     ANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            298..1395
                     /locus_tag="OO335_004327"
     CDS             298..1395
                     /locus_tag="OO335_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6004435.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          437 a          320 c          346 g          309 t
ORIGIN      
        1 cgcttatccg atttttgatc gtattgaaga aatggcatgg gctcgtcact accagcagat
       61 cgttcgtgaa gaaaaagaaa cggaactggc ggacgacctg gaaaaaggtc tgcctcagca
      121 cctgtttgaa tcgctctgca tcgaccattt gcaacgccac ggtgccaata aacaggcaat
      181 cagccacgca tttgatgatg atgttgagtt tcaggagcgc atggcagaac acatccggta
      241 catggctgaa accattgccc accaccaggt tgatattgat ttggaggact aaaatctatg
      301 gcgctttttc aacgagcaat aaacacacag gcctatctaa aagcaggaat tatggggttt
      361 gctggcgacg gcaaaaccta cacagcaagc gaactggcta ttggtctggt aatgcttatg
      421 cggcaacggg gaattgaagc tggtaaccgc ccggtaatgt ttctggacac agaaacaggc
      481 tctgactggg ttaaaccccg ctttgatgcg gaaaatatcg aattatttac ggcaaaaacc
      541 cgctcgtttg ttgatctgct tgaagccatt aatgaagcgg aaagcagcgg ttccgtaatg
      601 atcatcgact ccatcagtca cttctggacc ggattatgcg acgagtatgc cagacgccga
      661 aaccgtaaac gaggactgga attttcagac tgggcgtggc tcaaacagga gtggcgacgc
      721 tttaccgatc gatttgttaa cagtcaggcg cacattatta tgtgcgggcg tgcaggatat
      781 gagtatgact ttttcgaagg tgatgatggc aaacgccagt tggcaaaaac cggtataaaa
      841 atgaaagcag aaacagaaac cggttatgaa ccctccatcc ggatccagat ggagaagcaa
      901 atgaacatag aaaccggtca ggtgtggcgc accgccagaa tactaaaaga tcgctccacc
      961 cgtattgatg gacaggtatt ttcaaaccca acatttaaaa actttcttcc tcatatcgag
     1021 tctcttaacc tgggaggtga acaccccggc atcgatacct cacgggataa cagtgaatta
     1081 tttgccaacg acggtacacc gacatggcta aaagaaaagc gtgcaaaaga aatcgcactt
     1141 gatgaaatta tcgagttgct caacaagcac catgggggca ccagtaacga cgccaaacgc
     1201 gcaaaagctg atttgctgga aaaaacattc acctcccgct catgggaaag aatcaaaggt
     1261 atggactggc cgacaatcaa agctggtcgt aatgcactat ggattgaact ggaaggcgtt
     1321 gaatatgcat tcccggatcc acaaacacaa aacgaaacgc aacaagcagg atacgatgag
     1381 aatattccgg tgtaaaacca agtgcatttt gg
//
LOCUS       DAJTSF010000288         1404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000288 DAJTSF010000000
VERSION     DAJTSF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.297"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..167
                     /locus_tag="OO335_004328"
     CDS             <1..167
                     /locus_tag="OO335_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR6004436.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="OO335_004329"
     CDS             152..790
                     /locus_tag="OO335_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR6004437.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1018..>1404
                     /locus_tag="OO335_004330"
     CDS             1018..>1404
                     /locus_tag="OO335_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004438.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          347 g          393 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata
     1201 tttattaata aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa
     1261 agaagagata tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta
     1321 tttgatcatg acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagt
//
LOCUS       DAJTSF010000289         1398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000289 DAJTSF010000000
VERSION     DAJTSF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.298"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            62..736
                     /locus_tag="OO335_004331"
     CDS             62..736
                     /locus_tag="OO335_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR6004439.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     FKVRLAKQALQPGAVVARIAREHDINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKHDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIRE
                     MKGGIR"
     gene            733..1083
                     /gene="tnpB"
                     /locus_tag="OO335_004332"
     CDS             733..1083
                     /gene="tnpB"
                     /locus_tag="OO335_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6004440.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFTKRLAGDPGRESAPDASSVIHATGGDRVATSQTDR
                     TAWHPDITRDKTRE"
     gene            1080..>1398
                     /locus_tag="OO335_004333"
     CDS             1080..>1398
                     /locus_tag="OO335_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6004441.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          379 a          356 c          359 g          304 t
ORIGIN      
        1 tcttgtgaaa attgtcctgt ctggcaacaa tcgcgcccat ctatattgat ggacacgaac
       61 gatgaattcc cagacaacaa aagatattcc ctgcttccgt tcttatttgc ctgatgccct
      121 gcgtttaaga tttgaagata aactgaccat ccgggccatc gctcagcgtc tgggtctcag
      181 tcattccaca atacatacgc tttttcagcg atttcttgca tccggtatcg catggccatt
      241 gcccgattca gtttcattcg ctcaacttga cgccatcctt tatgccaaca gaaagaagga
      301 attaacagag cctcaaatca gagaaggctc atggcgaaaa gaacggcgaa ccagctatag
      361 ccgtgaattt aaggtccgtc tggctaagca ggcattacag cctggggctg ttgttgcccg
      421 gatcgccaga gaacacgata tcaatgataa cctgctgttt aaatggaaaa gccagtacga
      481 ggacggctta ctgagcgatg atgacataca ggaatgcatg cctgtcccgg tggcactgac
      541 tgatacgccg gagccgacca gaccagttac aaatcccttc tggcgtaaca agcatgatga
      601 gcgccctgag ggggctcccg gaaacgtccc acggtgcgag ctgcatctta aatcaggtgt
      661 ggtaaaactg tttgaccctc tcactccgga actgttacgg gcgctaatcc gcgaaatgaa
      721 agggggtatc cgatgataac gctgccgacc ggtaccaaaa tctggatcat cgctggcatc
      781 acagatatgc gttgtggctt caatggcctg gcttcgaagg tgcagaacac gctgaaagat
      841 gacccgttct ccgggcatat cttcgtcttc cggggccgca gtggcaaaat ggtgaaaata
      901 ctgtgggccg atcgtgacgg gttatgcctg ttcaccaaac gtctggccgg tgacccggga
      961 agggaaagtg cacctgacgc cagctcagtt atccatgcta ctggagggga tcgcgtggca
     1021 acatcccaaa cggacagaac ggcctggcat ccggatataa cccgtgataa aacaagggaa
     1081 tgaacaatga actccccgat gatattgagc tgcttaaagc catgttgcgt aagcaacaga
     1141 gtcggcttcg acagtatgcc tgtcaggtcg cgggctatga gcaggaaatt gaacggctga
     1201 aagcgcaact cgacaggttg cgtcgtatgt tgttcggcca gagttcagag aaaaagcgtc
     1261 ataagcttga aaatcagatc cgacaggcag aaaaacgact gtcggaactg gaaaaccggc
     1321 tgaacacagc cagaaatctt ctggaagatg catcgtcagt cacagattca cctgacacca
     1381 gtcccccgtc agaaaacc
//
LOCUS       DAJTSF010000290         1356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000290 DAJTSF010000000
VERSION     DAJTSF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.299"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(3..485)
                     /locus_tag="OO335_004334"
     CDS             complement(3..485)
                     /locus_tag="OO335_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6004442.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="OO335_004335"
     CDS             complement(476..778)
                     /locus_tag="OO335_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6004443.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1050..1322
                     /locus_tag="OO335_004336"
     CDS             1050..1322
                     /locus_tag="OO335_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004444.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          398 a          342 c          288 g          328 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgcctg aaccacggca gcctggcggc tgcaaatcgg ggtgtggcag aatatgagct
     1021 ttcagagtaa ccatccagaa tgagttccca tgtcacacaa cctggagcac cagaaagtcc
     1081 acaccagaat ggtgaaagag gttctgaaag ccgtcgccag agccaataat cacccttata
     1141 agtccgtatt tgcagacttt atcaccggtc atccctcctg cacggtctgt ttctgggaaa
     1201 ccttccacaa aatgtacccg gattcaccgt acgaatacgt cacgttctgc catacctgcc
     1261 gtcgttttga tttatacgaa acagaagcgg agatgaaggc ggatgacccg aagtggtggt
     1321 gattttcagc cccgaccgct gaaacgcctg ttcaca
//
LOCUS       DAJTSF010000291         1337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000291 DAJTSF010000000
VERSION     DAJTSF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.300"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(208..363)
                     /gene="hokD"
                     /locus_tag="OO335_004337"
     CDS             complement(208..363)
                     /gene="hokD"
                     /locus_tag="OO335_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6004445.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(591..1256)
                     /locus_tag="OO335_004338"
     CDS             complement(591..1256)
                     /locus_tag="OO335_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR6004446.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          331 a          342 c          314 g          350 t
ORIGIN      
        1 tccatccctc ttctccctgc gcgctactta agtgagtcga ttctatctgc gaaggtgcgc
       61 gaatcaaatt caccggaaat aaacaacaaa aaacccgccg aaacgctgag agatcacagc
      121 gatgggcggg ttaagtgcgg gtgcgttgag gatgcctgac acatcagagg tgggcgggga
      181 tgggatcagc tccccacccg gtcactctta cttcctggat tcgtagtcta cgaagacagc
      241 gacctccgtc tggccggttc ggattcgtac ctcacagagg tctttcctcg ttaccagtgc
      301 cgtcactatg acggttaaac agatgacgat cagggcgatt aacatcgcct tttgctgctt
      361 catagcctgc ttctccttgc ctttcggcat gtaagaggct aacctacatg tgcaaagcat
      421 gaaattggcc tcagattaat gttaagcgtc ttgccggacg cgtaatgtta actggggctt
      481 ttctctatct gccttttggt gttcatgcct gaagcagata gcctcaagca cccgcagtca
      541 ttctacttac ctcgccaata tgaaatcaat cagaaaggtg ccccataaaa tcactccttc
      601 tcttctttac cgtagtggag ttgaccaatt ttgataagag ggcgtccctg agatttgcgg
      661 tgtagattgg tatcgcgcag agaatacaca cagccacaat attcctgctg atagaatttt
      721 tcgcgcttgc tgatttcaat catacgggac gagccgccct gcttgcgcca gttataatcc
      781 cagtacacca tgcccggata atgcgcgacg gctcgccgcc cacagtcgtt aacctgctgc
      841 atatttttcc agcgtgaaat gcccagtgaa ctgctgatca cactgaaacc attttcagcg
      901 gcgtacagcg ctgtccgctc aaaacgcatg tcaaaacaca tggtgcaacg gatccctcgt
      961 tcgggctccc attccattcc tttggctcgt tcaaaccagt tgtctgtgtc gtaatcagca
     1021 tcaataaacg gcacgccgtg ttgttcagca aagcgaatat tctcatcctt acgaattaaa
     1081 tactctttct gaggatgaat gttcgggttg tagaaaaaga tggtgtagtc gatttccgag
     1141 gcctgaagcg cctccatcac ctcaccggaa catggagcac agcaagagtg cagtagtagt
     1201 ttgtttgccc cgtttgggag ctccaatttg ggccgtttga aatcagcaac tgtcataaat
     1261 atgtttattg aggtcataaa aatatcaaag agtgtagcat tagaacgggg ctatcggaaa
     1321 cagatgtgtg actgctc
//
LOCUS       DAJTSF010000292         1277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000292 DAJTSF010000000
VERSION     DAJTSF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.301"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO335_004339"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO335_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR6004447.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          358 a          229 c          286 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DAJTSF010000293         1275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000293 DAJTSF010000000
VERSION     DAJTSF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.302"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            34..435
                     /locus_tag="OO335_004340"
     CDS             34..435
                     /locus_tag="OO335_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6004448.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OO335_004341"
     CDS             447..818
                     /locus_tag="OO335_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6004449.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1275
                     /locus_tag="OO335_004342"
     CDS             805..>1275
                     /locus_tag="OO335_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6004450.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          286 c          396 g          290 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atccgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccg
//
LOCUS       DAJTSF010000294         1241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000294 DAJTSF010000000
VERSION     DAJTSF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.303"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..131
                     /locus_tag="OO335_004343"
     CDS             <1..131
                     /locus_tag="OO335_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCR6004451.1"
                     /translation="ERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            246..>1241
                     /locus_tag="OO335_004344"
     CDS             246..>1241
                     /locus_tag="OO335_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR6004452.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRGIYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSP"
BASE COUNT          336 a          318 c          331 g          256 t
ORIGIN      
        1 ttgaacggtg ctttgggcgg ctgaaagaat atcgccgcat cgccacgcgt tacgacaaaa
       61 cggcgagaaa ttacctggcg atggtgaaac tgggctgcat ccgactcttt tatcaacgct
      121 tacgtaatta agggacacag cctaaatagc tgcgcggaat agtagatccc tttgagggaa
      181 cttagcccgg attgtgcgat ctgatcaatc gccaaatcaa aacaaatcac caaccggact
      241 gagcaatgcc gatcatagca ccaatttccc gtgacgaacg acgcctgatg cagaaagcca
      301 tccataaaac acacgataaa aattatgccc gcagactgac tgccatgctg atgctgcacc
      361 ggggcgaccg tgtcagcgac gttgccagaa cgctctgctg cgcccgttcc tctgttggac
      421 gctggattaa ctggttcacg cagtcgggtg ttgagggact gaaatcatta cctgccgggc
      481 gtgcccgtcg ctggccgttt gagcatatct gcacactgtt acgtgagctg gtaaaacatt
      541 ctcccggcga ctttggctac cagcgttcac gctggagtac agaactgctg gcaataaaaa
      601 tcaatgagat aaccggctgc cagttaaatg ccggaaccgt tcgccgctgg ttgccgtctg
      661 cggggattgt gtggcgaagg gctgcgccaa ctctgcgtat ccgtgacccg cataaagatg
      721 aaaagatggc agcaatccat aaagcactgg acgaatgcag cgcagagcat ccggtctttt
      781 atgaagatga agtggatatc catcttcatc ccaaaatcgg tgcggactgg caactgcgcg
      841 gacagcaaaa acgggtggtc acgccgggac agaatgaaaa atattatctg gccggagcgc
      901 tgcacagcgg gacaggtaaa gtcagctatg tgggcggcaa cagcaaaagt tcggcgctgt
      961 tcatcagcct gctgaagcgg cttaaagcga cataccgtcg tgcgaaaacc atcacgctga
     1021 tcgtggacaa ctacattatc cacaaaagcc gggaaacaca gagctggctg aaggagaacc
     1081 cgaagttcag gggcatttat cagccggttt actcgccatg ggtgaatcat gttgaacggc
     1141 tatggcaggc acttcacgac acaataacgc gtaatcatca gtgccgctca atgtggcaac
     1201 tgttgaaaaa agttcgccat tttatggaaa ccgtcagccc a
//
LOCUS       DAJTSF010000295         1160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000295 DAJTSF010000000
VERSION     DAJTSF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.304"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            115..>1160
                     /gene="cadB"
                     /locus_tag="OO335_004345"
     CDS             115..>1160
                     /gene="cadB"
                     /locus_tag="OO335_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCR6004453.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLADIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          248 a          255 c          297 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcagat attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctga
//
LOCUS       DAJTSF010000296         1140 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000296 DAJTSF010000000
VERSION     DAJTSF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.305"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..730
                     /locus_tag="OO335_004346"
     CDS             <1..730
                     /locus_tag="OO335_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR6004454.1"
                     /translation="TDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVN
                     KPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT
                     GICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGV
                     HNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRP
                     LPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(732..958)
                     /locus_tag="OO335_004347"
                     /pseudo
     CDS             complement(732..958)
                     /locus_tag="OO335_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          298 a          283 c          247 g          312 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt
      541 gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc
      601 ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc
      661 ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga
      721 agacgaatga cttactgaaa aaacaccaca atcttgttaa acatcgtcgg gttggactga
      781 ttacgttgca ctttcaccac atattccagc ttatctattt ggcttatcac ctactccaga
      841 cgctggtcat ccttgaccag tagccagata tggcttttgt cgcagtcctg aatcggcagg
      901 cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg tgagtcatta
      961 cacccggatg gtaaacaggc acttccttgt tgtagaagcc gttatattgt ccataatgtc
     1021 tgggttttta atgcatactg ggtaacaacc ggacgaatct tcgaatatca ctggcgctgc
     1081 cagccggttt gaattcatct cacaaccctg catagagcga atctcctgcc tgcacgtcac
//
LOCUS       DAJTSF010000297         1109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000297 DAJTSF010000000
VERSION     DAJTSF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.308"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1019
                     /locus_tag="OO335_004348"
                     /pseudo
     CDS             1..1019
                     /locus_tag="OO335_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          312 a          271 c          258 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggtta
//
LOCUS       DAJTSF010000298         1100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000298 DAJTSF010000000
VERSION     DAJTSF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.309"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            55..>1100
                     /locus_tag="OO335_004349"
     CDS             55..>1100
                     /locus_tag="OO335_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6004455.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          344 a          255 c          178 g          323 t
ORIGIN      
        1 cattaggaga gatacattaa atacaattta ttcacaatct aattccatat ccatatgcga
       61 gaaattaata tgctcagaaa catttcatcc tgtttatttc cacatatcag cacaattaca
      121 tcccccaacc attatttgtc cgaatgggat gattgggaga aacaggggtt accggaagaa
      181 cagcgtactg aggcggtaag aagacttcgt gcatgtctta cctctaaggg gcataaactg
      241 gacctgcgag ccttggcgct ttcctcgcta cctgtactcc ctgcttgcat taaaaagctt
      301 gatgtgagct gtaataaatt aaccatcctt actgatttac ctgaaaatat taaagaactt
      361 attgcaagag ataatttctt aacacatata tctgcattac cacattatct aataactttg
      421 gatgtgtccg aaaatcaatt agagaatctg ccgttattac cagacaccat caaatcacta
      481 agcgcagagt ataataggtt atccacactg ccttcattac ccttgaattt aaaaaaactt
      541 gaggttagga acaacgaact gcaaactctt ccatctctgc cttctaatct taagatactt
      601 aaggttgcgc acaaccatct tactgaactg ccccctttac ctaggagact gcaacttctt
      661 tttgcatata gcaatagatt aagcaactta ccaaacatcc aagaaaatat tatcatgaga
      721 agattttttt attttgaaaa caaccaaata actacaatcc cgacaaatct ttttcgttta
      781 gatcctcata taactattga gattgcaaat aaccccttat cagatcaaac tctgctattc
      841 ttaatacagc aaacttcggt tccaaatttt aacgggcctc agtttcgtat ttccctgtca
      901 gaccaaaaca gactgttttt acgccagatg ttgccgcaaa atttacattc gcgccatatc
      961 agagtcatca ctgaaggggg gcagaacttt cagatccccc ctcttcccga aactgtggca
     1021 gcctggtttc ctgaagcaga tcgtcgggag gtttctacac aatggacttc tttttccacc
     1081 gaggagaatt cccgggcatt
//
LOCUS       DAJTSF010000299         1076 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000299 DAJTSF010000000
VERSION     DAJTSF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.310"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(348..1043)
                     /gene="aqpZ"
                     /locus_tag="OO335_004350"
     CDS             complement(348..1043)
                     /gene="aqpZ"
                     /locus_tag="OO335_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR6004456.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          304 a          322 c          252 g          198 t
ORIGIN      
        1 gatttttaat cctatctgag tgtttagcat tgttgcgctc ctttcatgtg caatgccata
       61 taaagccact ggcggaggga gcgcgcaggg ggcagccagt cgccgccgcc ccctgcaccc
      121 ccgggctctg gcgaacaaaa tcgccgctgc gcggtacctt cagcttatcc ccttcggcaa
      181 ccgggtcggg cgcgaggtaa cgtcctgtaa gacgcgccct cagcccacat ccatgtgggc
      241 tgccccggcc ttcggggaac gcttcagcga ttttgacgcc accaataccc gtgcggtttg
      301 taatgtcgaa gataaaacaa ccagggcaga taaaatgcca atgctaatta atcacgcttt
      361 tccagcagag tccggtaaat cagaccaccg ataatgccgc cgacaattgg caccacccag
      421 aagaaccaca gttgttctaa tgcccagccg ccctggaaga tagcaaccgc ggtgctgcgc
      481 gccgggttaa cagaagtgtt agtcaccgga atactgatta agtgaatcag ggttaaggcc
      541 agaccaatag cgatcggcgc aaaacctgcc ggagcgaatt tgtcggttgc accgtggatc
      601 actaacagga agcccgcact caataccagt tcaactacca gcgcggaaag catggaataa
      661 ccgcctggtg aatgctcgcc ataaccgtta gaagcaaaac cgctggctgc cgcgtcaaaa
      721 cccgttttac cactggcaat taaatacagc agcgccgctg caacaatacc gccgacaacc
      781 tgggcaatta cgtagccaac gacttctttt gccggaaaac gtccgccagc ccataaacca
      841 atagtgaccg ccgggttaaa atgaccacca gaaatatgac caacagcgaa ggccatcgtc
      901 agaacggtca aaccaaatgc cagcgccacg ccggcaaaac caatgcctaa ttccgggaag
      961 cctgcggcca gtacagcact accacagcca ccaaaaacaa gccagaaagt accaaaacat
     1021 tcagctgcta attttctgaa catatccacc tcaattaaaa attgaccctg tgaaaa
//
LOCUS       DAJTSF010000300         1069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000300 DAJTSF010000000
VERSION     DAJTSF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.311"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(227..652)
                     /locus_tag="OO335_004351"
     CDS             complement(227..652)
                     /locus_tag="OO335_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004457.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(649..1032)
                     /locus_tag="OO335_004352"
     CDS             complement(649..1032)
                     /locus_tag="OO335_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004458.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          253 a          308 c          265 g          243 t
ORIGIN      
        1 ctcgcgtatg gttgattgac accacagtgc gaattcggcg aatgcgagat cacggtacga
       61 ttccgcacca cactaccaat caataccccg ggttttattc aggtaagcag gactgcggat
      121 atccggtgtc gcgcctttgt gtcacgaacg gggtgggtgc gaaacaccag ataaaataca
      181 ggttggcaat acctctgaac gccctgagca ggtcgggcat ttcggattaa gtactcgcac
      241 ctccgcagtc ctcaaacaag tctggctggt agctgtaaac agacttcgta cataccgctc
      301 tgcaataaat ccccgtacca caggcctcac agaacttctt cccatgaaaa aactgctcac
      361 acatcacaca atgccactcc agcacgaccg gtaatggcga taaaaacatc gccatatcct
      421 caatgtaagg gtgggacttc tccggattca gcaccacaca gtccgccttc tgttgctcac
      481 tcagggcatg taaatcatgc tcaaaccacg ccccctgagc atctgtctgc agaatcaacc
      541 ggccatggca ggaaaggcag aaacactgcc ggatatttct gctgagcgca gagtcggcac
      601 tgataatgtg ctcacccggc gtgatcccca gccccgtttt tataccgttc attctgctcc
      661 cccctcctca ctgaagcgcc cggtctggca gtgtgtgcag tactgctccc cataataatc
      721 gtggtgacat tgcctgcagt gccagctggc tttacgtact acgggtaagg tatccgggac
      781 gaattgctgc agacgtttaa tcaaccgtat ttctctgcgt tccggcctga cataagggca
      841 ctcatgaccg tgctccgtca gcccttcgtc agcgtgttca aaccagggcc gttcagtgtc
      901 gtattgcggg tggtatctga gtgcactgcc gcaaaggtgg caggtgtagc tatcgtaagg
      961 tgcagtctgt gcggtacggg caccggtcag acgtccgttg ccatcaagtg cgagaaaaga
     1021 ttttgcgtac atagtatatg ttcctcagcc gccagacgac acgcaggca
//
LOCUS       DAJTSF010000301         1068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000301 DAJTSF010000000
VERSION     DAJTSF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.312"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            18..560
                     /locus_tag="OO335_004353"
     CDS             18..560
                     /locus_tag="OO335_004353"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004459.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
     gene            787..>1068
                     /locus_tag="OO335_004354"
     CDS             787..>1068
                     /locus_tag="OO335_004354"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HCR6004460.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          314 a          202 c          257 g          295 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggt
//
LOCUS       DAJTSF010000302         1066 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000302 DAJTSF010000000
VERSION     DAJTSF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.313"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(570..749)
                     /locus_tag="OO335_004355"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="OO335_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            843..>1066
                     /locus_tag="OO335_004356"
     CDS             843..>1066
                     /locus_tag="OO335_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6004461.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DAJTSF010000303         1036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000303 DAJTSF010000000
VERSION     DAJTSF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.314"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..576
                     /locus_tag="OO335_004357"
                     /pseudo
     CDS             1..576
                     /locus_tag="OO335_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          313 a          232 c          219 g          272 t
ORIGIN      
        1 actttacgcc cacagcacct aatcaggtct gggtcgcaga ttcagttgtc caggccttcc
       61 gaaatcaacc gactgaaggc gctggtcgcg aaactgcagc gtatgcagtt cggtaaaagc
      121 tcagaaaaac ttcgcgcaaa aaccgaacgg tggatacagg aagcacagga gcgaatcagc
      181 gcacttcagg aggaaatggc ggaaacactg ggtgagcaat atgacccggt actgccatct
      241 tccctgcgcc agtcttcagc ccgtaaaccg ttaccagcct cacttccccg tgccccccgg
      301 gtcatccggc cggaagagga atgctgtccg gcctgtggtg gtgaactcag tccactggga
      361 tgtgatgtgt cagagcagct ggagcttatc agcagcgcct ttaaggttat cgaaaaacaa
      421 cgccctaaac tggcctgtcg ccggtgcgac catatcgtgc aggcaccggt gccttcaaaa
      481 cccattgcac gcagttatgc cggagcgggg cttctggccc atgttgttac cggaaagtat
      541 gcagaccatc tgccattata ccgccaaagt gatctactat tccacacagc tatttagctc
      601 tctttttgtt gagctcccat gattctttca tttattgtta ctaattattt tatgttatca
      661 tgatgttaga acacgctctg ctctgtgtct ctattttatc tatcatattc tgaggattaa
      721 ctacataaca aggcgcaaga tatatataca aaaacaaata ataccgttgc aataattcct
      781 tttagtaaag gaggtgtgat taatttcagg ggatttcaga gtaaaaattg tacaacatat
      841 taaaatatgt tcatttcgtt ataatacaca caaggaaata ttaagttaga taatatattc
      901 taaataacca tagataaaaa cgcacataat ggggaatatt ttatgaaaat acctgaagca
      961 gtaaatcata ttaatgttca gaataatatt gatcttgttg atggaaaaac gaatccgaat
     1021 aaagcaacaa aagcat
//
LOCUS       DAJTSF010000304         1029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000304 DAJTSF010000000
VERSION     DAJTSF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.315"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(59..199)
                     /locus_tag="OO335_004358"
     CDS             complement(59..199)
                     /locus_tag="OO335_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004462.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            338..1009
                     /gene="queE"
                     /locus_tag="OO335_004359"
     CDS             338..1009
                     /gene="queE"
                     /locus_tag="OO335_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR6004463.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          302 a          206 c          239 g          282 t
ORIGIN      
        1 aacaaaggaa catacgaaag tgaacattag tatgaaatag cgcattataa atttctcatt
       61 aaatctggat aaataaattg acggcggcat cggtaatttt agattcgtta tcattaaaaa
      121 ctaatacaga aaaaataaat ataattttgg tgaatgtttt gacatgatta aatccatttt
      181 ctttattttc ttctgacata atacgttact ccctttaatt attaacgata tgtttcgtat
      241 ttttctttaa atctgcatgt aacagaatgt taataagaca tgattgttgc ggcaggatct
      301 gcaataatta acaattatcc acttcacaga gaatgctatg cagtacccga ttaacgagat
      361 gttccagacc ctgcaaggtg agggttactt taccggcgtt cccgccattt ttattcgttt
      421 acagggatgc ccggttggct gtgcctggtg cgacaccaaa cacacctggg aaaagcttga
      481 ggatcgggaa gtctcccttt tcagcattct ggcgaagacc aaagagagtg ataagtgggg
      541 ggcggcgagc agtgaagact tgctggctgt cattggtcgt cagggatata ccgcgcggca
      601 tgtggtgatt acgggtggtg agccttgcat tcatgatttg ctgccactga ctgacctgct
      661 cgaaaagaac ggttttagct gccagatcga aaccagtgga actcatgaag tacgctgcac
      721 accgaatacc tgggttaccg tatcgccaaa gctgaacatg cgcggcggct atgaagtgtt
      781 gtcacaggca ctggagcgag ccaacgaaat caagcatcca gtggggcgcg tacgcgatat
      841 tgaagcactg gatgaactac tggcaacgct gaccgatgat aaaccgcgag tcattgcact
      901 gcagccgatt agccaaaagg atgatgccac acgattgtgc attgaaacct gtattgcgcg
      961 taattggcgt ttgtcgatgc aaacacataa atatttaaat attgcctgat tacacattta
     1021 taagcgtta
//
LOCUS       DAJTSF010000305         1006 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000305 DAJTSF010000000
VERSION     DAJTSF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.316"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            214..711
                     /locus_tag="OO335_004360"
                     /pseudo
     CDS             214..711
                     /locus_tag="OO335_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            752..943
                     /locus_tag="OO335_004361"
                     /pseudo
     CDS             752..943
                     /locus_tag="OO335_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          265 a          247 c          291 g          203 t
ORIGIN      
        1 ggtggactgg tataacaatc gacgattgct ggaaaggctg ggccatactc ctccggcaga
       61 agcagaaaag cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga
      121 taaaacactc tccaggaaac ccggggcggt tcaaagcgcc agccccgtcc cgcccgatag
      181 agcgcggcat agcgggcccc ggattacttg cgtgcgacgg cagaagaagc cctgaagcga
      241 atcagtgaac tgtacgccat cgaggatgaa atacggggat taccagagtc agagtgtctt
      301 gcagtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgcatgaatg gatggtggag
      361 aagaatggca cgctgtcgaa aaaatccaga ctgggcgaag cattcagcta tgtactgaat
      421 cagtgggacg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      481 gcggaaagag cgcttcgtgc agtctgtctc ggaaagaaaa attacgtgtt cttcggtagc
      541 gatcacggcg gcgagcgtgg tgcactgctg tacgggctga tcggcacctg ccgtctgaac
      601 ggtatcgatc cggaagcgta tctgcgccat attctgagcg tactgccgga atggccttcc
      661 aaccgagttg acgacctcct gccatggaag gtggtgctac catcaggata atcgtcaata
      721 cggtgttcgg tgaacgttta cttggagcat catgcttcat gaagaaaaac tggcacgtca
      781 tcaacgtaaa caggcgatgt atacccgaat ggcagccttc ccggcggtga aaatgttcga
      841 agagtatgac ttcacgttcg ccaccggagc accgcagaag cagctccagt cgttacgctc
      901 actcagcttc atagagcgca atgaaaacat agtcttactg gggacatctg acatcacaaa
      961 tccccgggtg gggatttgtg tataagagac agcccatgga ggaagg
//
LOCUS       DAJTSF010000306          990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000306 DAJTSF010000000
VERSION     DAJTSF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.317"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            82..>990
                     /locus_tag="OO335_004362"
     CDS             82..>990
                     /locus_tag="OO335_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR6004464.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          301 a          206 c          228 g          255 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt
//
LOCUS       DAJTSF010000307          983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000307 DAJTSF010000000
VERSION     DAJTSF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.318"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO335_004363"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO335_004363"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR6004465.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          220 c          222 g          282 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DAJTSF010000308          978 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000308 DAJTSF010000000
VERSION     DAJTSF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.319"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            422..>978
                     /locus_tag="OO335_004364"
     CDS             422..>978
                     /locus_tag="OO335_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6004466.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          352 a          188 c          157 g          281 t
ORIGIN      
        1 aaaacaaagc caacggtatt ctgatttctg aagttaattt cagactcgtt taagctccat
       61 tttcaaaaaa aaatatatca gggtttgtta ctaataactt ctgaaaacag aaaaaaatag
      121 agcagtaatt ctatttcaga gaggagtaac tccatctatc ctcgatacgt actgaacgtt
      181 atcgtgatat attcatcatg ctttcttcac cattattcga gtatagggag agacgcaatg
      241 accttaaata cctttttatg gctgaattac cattctcagg aataacagca gactccttcc
      301 catagacggc ttagggggaa agcaataccc ggagaaaggg tacaaagcaa actgttttaa
      361 aattaataag ccatattcta tttaactaga attcttatcg ttgataagaa ttctagttat
      421 aatgattaaa tcaaccaata tacaggcaat cggttctggc attatgcatc aaataaacaa
      481 tgtatactcg ttaactccat tatctttacc tatggaactg actccatctt gtaatgaatt
      541 ttatttaaaa acctggagcg aatgggaaaa gaacggtacc ccaggcgagc aacgcaatat
      601 cgccttcaat aggctgaaaa tatgtttaca aaatcaagag gcagaattaa atttatctga
      661 gttagattta aaaacattac cagatttacc gcctcagata acaacactgg aaataagaaa
      721 aaacctatta acacatctcc ctgatttacc accaatgctt aaggtaatac atgctcaatt
      781 taatcaactg gaaagcttac ctgccttacc cgagacgtta gaagagctta atgcgggtga
      841 taacaagata aaagaattac catttcttcc tgaaaatcta actcatttac gggttcataa
      901 taaccgattg catattctgc cactattgcc accggaacta aaattactgg tagtttctgg
      961 aaacagatta gacagcat
//
LOCUS       DAJTSF010000309          977 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000309 DAJTSF010000000
VERSION     DAJTSF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.320"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..200
                     /locus_tag="OO335_004365"
                     /pseudo
     CDS             1..200
                     /locus_tag="OO335_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            300..926
                     /locus_tag="OO335_004366"
                     /pseudo
     CDS             300..926
                     /locus_tag="OO335_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
BASE COUNT          305 a          228 c          218 g          226 t
ORIGIN      
        1 attggctacg taattaaaac agaaaataaa gaaaccccat tcactgtagt tgatatcgac
       61 ggtctatcag gaaactttaa aactcttgtt gaaggtgcca caaaaatgtg cctagtttac
      121 atcggaaata atctgcccgc agaaaaaaag ccgaatttct ggcaaatctg attgcaatga
      181 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacag tgtgcaggcc tgagtgaaga
      241 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
      301 tgagcacaaa accactcttc ctgttacgga aagcgaaaaa atcatccggt gaacctgacg
      361 tcgtcctgtg ggcaagcgac gattttgaat cgaccagcac cactctggac tatctgctcg
      421 ttaagtcagg taaaaaactg agcaactatt ttaaagctgt tgccacaaat tttcctgtcg
      481 ttaatgacct tccccctgaa ggtgagatcg attttacctg gagtgaacgc tatcaactca
      541 gcaaagactc catgacatgg gaactaaaac cgggagcagc gccagacgac gttcaccacc
      601 aggataatgc tcaagaaacc aaagaactgg cgggaggcca ggaagaaaac gcgcaggcag
      661 acgcccacga ggattgccag gattgcgaag tctctgtagc cactttgcgg ttcacacagc
      721 gtcttctgca catttttacg tatgctgccg gggatctgaa atacctgcat catgccaccc
      781 gtgaacaacg caaacacatt actgctcttg agatggatca ggaaaacagc tatgtccaga
      841 atctgctgtt ggccatacgc ggcatggcag aaccgacaac tctggataat gccgccctgc
      901 tccgcctgac tgatgcaatt aaggctgagg tgtactggca atagcggaca ctaccatttg
      961 ttcttttttt aagcagc
//
LOCUS       DAJTSF010000310          933 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000310 DAJTSF010000000
VERSION     DAJTSF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.321"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(55..243)
                     /locus_tag="OO335_004367"
     CDS             complement(55..243)
                     /locus_tag="OO335_004367"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004467.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
BASE COUNT          245 a          181 c          217 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aag
//
LOCUS       DAJTSF010000311          915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000311 DAJTSF010000000
VERSION     DAJTSF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.322"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..496
                     /locus_tag="OO335_004368"
     CDS             <1..496
                     /locus_tag="OO335_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6004468.1"
                     /translation="LHASTNAPGSDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQK
                     THYSDTRWHEDSVRNRWFSVMAGLSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDN
                     KGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGV
                     GYKF"
     gene            518..>915
                     /locus_tag="OO335_004369"
     CDS             518..>915
                     /locus_tag="OO335_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6004469.1"
                     /translation="MTARWFRRAFCGVNMAVKISGVLKDGAGKPVVNCAIELRARRTS
                     PTVVAHVVATCVTDNNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNA
                     FLNAPKDGDLRPEVMKRFEEMVAQAQQSAGA"
BASE COUNT          199 a          207 c          300 g          209 t
ORIGIN      
        1 tcttcatgcc tcgacgaacg ctcccggcag tgatgatctg aacgggatta acgtgaaata
       61 ccgttatgag tttacggaca cgctggggct ggtgacgtca ttcagctatg ccaacgctga
      121 agatgagcaa aaaacgcatt acagtgatac ccgctggcat gaggattccg tgcgtaaccg
      181 ctggttcagc gtgatggcgg ggctgtctgt gcgcgtgaat gaatggttca gcgcgtatgc
      241 gatggcgggc gtggcttaca gccgtgtgtc gaccttctcc ggggattatc tccgcgtaac
      301 tgacaacaag gggaaaacgc acgatgtgct gaccgggagt gatgacaacc gccacagcaa
      361 cacgtctctg gcgtgggggg ctggcgtgca gtttaacccg accgaatccg tggccattga
      421 ccttgcttat gaaggttccg gcagtggcga ctggcgcact gacggtttca tcgtgggtgt
      481 cggttataag ttctgattag ccaggtaaca cagtgttatg acagcccgct ggttcaggcg
      541 ggctttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag atggggcggg
      601 taaacctgtc gtaaattgtg cgattgaact gcgggccaga agaaccagtc cgaccgttgt
      661 ggcacacgtt gttgccactt gcgtgacgga caataacggt gcttatgtga ttgaggctga
      721 gccggggtat tacgaggttg cgcttcactg taacggctgg cagccaaccc gtgtcgggga
      781 tattgatgtg gcaccgactg atgcaccggg gacactgaac gcgtttctga atgcaccaaa
      841 ggatggtgat ttacgtccgg aggtgatgaa gcgctttgag gaaatggtgg cgcaggcgca
      901 gcagagtgcc ggggc
//
LOCUS       DAJTSF010000312          902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000312 DAJTSF010000000
VERSION     DAJTSF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.323"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OO335_004370"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OO335_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR6004470.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          265 a          145 c          221 g          271 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ct
//
LOCUS       DAJTSF010000313          870 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000313 DAJTSF010000000
VERSION     DAJTSF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.324"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>870
                     /locus_tag="OO335_004371"
     CDS             <1..>870
                     /locus_tag="OO335_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6004471.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTL
                     AYRPLNDTQRKIV"
BASE COUNT          263 a          153 c          209 g          245 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga
//
LOCUS       DAJTSF010000314          864 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000314 DAJTSF010000000
VERSION     DAJTSF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.325"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..153
                     /locus_tag="OO335_004372"
     CDS             <1..153
                     /locus_tag="OO335_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6004472.1"
                     /translation="MRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQ
                     KKEYQA"
     gene            435..716
                     /locus_tag="OO335_004373"
                     /pseudo
     CDS             435..716
                     /locus_tag="OO335_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
BASE COUNT          282 a          179 c          198 g          205 t
ORIGIN      
        1 atgcgacttg aacgcaagca tggaaaactt gctgagtggg ttcgggagca actcaacaag
       61 aagcattcga atgtggttac ctgcgctctg gcaaataaac tggctcgaat tgcctgggca
      121 atcactactc agaaaaaaga atatcaggcc tgatatagat gctgatataa ctcaattagt
      181 ttattttaaa caataagata caagttacct ttctggtttt gcgaagactg attattgatg
      241 acatgaacgg caaatcgacc tgattaaaaa cctaacagat gaaatggctc actgaagccg
      301 aggggatttt taggattatc aggtgcggaa ctcatcgggg cgcgggcatc cttgcccaca
      361 gagacgccgg atagatttaa gcaagccaac cattaatcaa aaatcagtgt tgcaaaaacg
      421 ggggtgacca tagatttcat cttatcaccg gacaacaggc tgaccgactc gccgatctgg
      481 aaagaatgtt gcacctgttc agcggtaaac caatccccga caaccgggga aacatcaccc
      541 ttcgtctgga tgatcacatt cagtctgttc agggtaaaga gagctatgaa gatgagatgt
      601 tcagcatcag atactttaag aagggctccg cgcatatcac gttcagaaaa ccagaactgg
      661 ttgacagact gaatgatatt atcgcaaaac actatcctga tatgctggca gtatagcttt
      721 gccagtatgc tcaaagatcg gaaattgtcc gataagatat gatcgcgggg catataaacc
      781 ggtttcagat ggcatacccg atggcatact gtctggaagg aaaaataaaa aagcgcatgt
      841 taaacaataa catgcactta taat
//
LOCUS       DAJTSF010000315          832 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000315 DAJTSF010000000
VERSION     DAJTSF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.326"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..327
                     /locus_tag="OO335_004374"
     CDS             <1..327
                     /locus_tag="OO335_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR6004473.1"
                     /translation="VSETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNG
                     LLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVI
                     LKITYN"
     gene            complement(370..570)
                     /gene="glgS"
                     /locus_tag="OO335_004375"
     CDS             complement(370..570)
                     /gene="glgS"
                     /locus_tag="OO335_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR6004474.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          253 a          184 c          154 g          241 t
ORIGIN      
        1 gttagtgaga cgtttagtgt agtcatgaca aaagatcctg gtgcggcctg tactgagcaa
       61 ttcaatattt tagggagctt tttcactacg gatattttga gtgattatag ccatctggat
      121 atgggtaatg gactgctatt gaagatattt cataaagatg gaacagcaac ggaatttaac
      181 cgcttctcac aatttgcttc tttttcatcg tctagtgcac cttcggtcac cgcaccattc
      241 agggcagaac ttagtgcgaa cccggcagag actgttgttg agggaccgtt tagtaaagac
      301 gtaatcctca aaatcaccta taactagcat ctaatacaaa cactaaaacg ggccatcagg
      361 cccgttatat cagtgctcta actccagctc tcttgcctgc atcatctgtt ggcaatacca
      421 agcataacgt gcgcaaaacc aagttctatg ttcttcatcc atgttaccag taacagccag
      481 aatatcgacc gggcgacctt ctgcgtgcat tctggcaaaa ctacgcgcca ggaaatcgaa
      541 attattcaaa gaattaagac tatgctccat aagcacatcc tccggttatc gcataccttt
      601 taaggccata tgctttttct gatttcagta ttccccaatc agtgtagtgc ctttaaacat
      661 aacgtgcgta aatatattgt ccccgatcac actttttagt gaaaaataaa aaaccatatt
      721 aaaatacttt ctcttccaga acttctctaa catgtgttat tattaacaaa gacaaatgta
      781 agcagggaaa ctattccaag gtaaatattg cgtcactaaa tggacattgg ag
//
LOCUS       DAJTSF010000316          780 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000316 DAJTSF010000000
VERSION     DAJTSF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.327"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..129)
                     /locus_tag="OO335_004376"
     CDS             complement(<1..129)
                     /locus_tag="OO335_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6004475.1"
                     /translation="MSRKTQRYSTEFKAEAVKTVPENQLSISEGASRLSVPEGTLGQ"
     gene            complement(467..>780)
                     /locus_tag="OO335_004377"
     CDS             complement(467..>780)
                     /locus_tag="OO335_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR6004476.1"
                     /translation="KADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDG
                     ANYKVLEYFINNGLVDVNKKFQKVNSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFE
                     I"
BASE COUNT          215 a          165 c          140 g          260 t
ORIGIN      
        1 ttgtcctaaa gtgccttcag gaacagataa tcgggaagct ccttcactga tcgaaagctg
       61 attttcagga accgttttga cggcttcggc tttaaactcc gtagagtaac gttgggtttt
      121 tctgctcatt attggctcct tctgatgcca ttctatttca ggaaggagtg tccgttaaac
      181 tcaggctacc tcactcccgt aaagacagga gtcaagttcc cgctccgaca taactgcgtg
      241 caatggattt tgaaggaacc gtcagcccat tccccggagg caaacatggg ctggcaaaag
      301 tgtagcggta ttaggcacag ctatttagga aagagggacc atctccatct cattaaatgg
      361 accacgccag acaattcact gtcatgctga tgctgcactg tggggacaga atcactcatg
      421 atgacttcga taatcgacga cattattatt tggccgagct tttaggtcat atctcaaatc
      481 gtttgcctaa tattgctcca ttttttaata aaaaatcaat catttttgaa tctttgcttt
      541 tcattgcatt gtcaagcata gtgtccccac tatttacttt ttgaaatttt ttgtttacat
      601 caaccaatcc attattgata aaatattcta gcactttata gttggcacca tctttactaa
      661 gtaagtattc aacatcatac agattacaat atctattgtt tttcattttc tccatctctg
      721 cgacatcttg ttttgtaaaa ttaaatttag atagtaaata aagtgctata tcagcttttt
//
LOCUS       DAJTSF010000317          777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000317 DAJTSF010000000
VERSION     DAJTSF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.328"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..193
                     /locus_tag="OO335_004378"
     CDS             <1..193
                     /locus_tag="OO335_004378"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004477.1"
                     /translation="FFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVAPPF"
     gene            193..>777
                     /locus_tag="OO335_004379"
     CDS             193..>777
                     /locus_tag="OO335_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR6004478.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITR"
BASE COUNT          164 a          202 c          233 g          178 t
ORIGIN      
        1 gttctttgtg gatgcgggaa acaaaaagct ggtcttctgg tttgtacgtg ttgatgacga
       61 aggatatccg gaaatcgccc gctgcacgga gcgggaattt gcgaccattc ttgccggtat
      121 cagcgccggc ggtatgtact gcccggagtg tggcacggtt cactggccgg acggagtcgc
      181 cccgcccttc tgatgctccc ccgttttgcc gatatttttc agcagggaaa ccgctggctt
      241 aactggctgg agaaacaacc ggaaggggca gtccgtccgg tggtaattga atcagtgaca
      301 aaaatcatgg cctgcgggac cacactgatg ggctacacgc agtggtgttg ttcatcaccg
      361 gactgttgcc atacaaaaaa ggtctgcttc cggtgtaaaa gccgctcctg cccgcactgc
      421 ggggtgaagg ctggcgcaca gtggatacag tatctgctga gcctggtccc ctactgcccg
      481 tggcagcata ttgtgttcac acttccctgc cagtactggt ccctggtgtt ccacaaccgg
      541 tggttactgg cagagatgag ccgcattgca gcggatgtga tactggaaat ctgccatcag
      601 gcagatgtgg agccggggat attcacggtg atccacacat gggggcgtga ccagcagtgg
      661 catccgcata tccatttatc gacaactgcc ggtggtgtga cgtcgggcca cacctggaaa
      721 aatcttcatt tttacgcccg taaggtgatg agcatgtggc gctaccggat aacacgg
//
LOCUS       DAJTSF010000318          759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000318 DAJTSF010000000
VERSION     DAJTSF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.329"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>759
                     /locus_tag="OO335_004380"
     CDS             <1..>759
                     /locus_tag="OO335_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6004479.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          184 g          209 t
ORIGIN      
        1 gagcgcattc tgaccgaccg tgaaaatggt gaaaaaggcg ataattctca agatacctct
       61 tatgcccgtg taggcgtgaa aggtgaaact cagatcaacc ctgaaatgac tggttacggt
      121 cagtttgaac tagacctgga agcatcaaac agacacaacc ctgaccaaac tcgtttggca
      181 tatgctggtt tgagttacaa agattttggt tcattcgact acagtcgtaa tgtcggtgtc
      241 gcgtatgatg cagaagcatt tactgatatg tttgttgagt ggggcggtga ctcatgggct
      301 ggtactgacc tgttcatgac caaccgtacc aacggtgttg caacctatcg taacacagat
      361 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
      421 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaactta cactattgac
      481 ggtttctcat ttgctggtgc ttatgcaaac tctgaccgta ctgattggca gtctggtgat
      541 ggtaaaggcg aaagagccga agtttgggcg ttgtcaacaa aatatgacgc taacaatgtt
      601 tatgctgctg tgatgtacgg tgaatctcac aacatgaatt ccgacgacgg tgatgttgtg
      661 aacaaaacac aaaacttcga agctgttctg caatatcagt tcgactttgg tctgcgtcca
      721 tccatcggtt actcctattc taaagcactg gacgtagca
//
LOCUS       DAJTSF010000319          726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000319 DAJTSF010000000
VERSION     DAJTSF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.330"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>726
                     /locus_tag="OO335_004381"
     CDS             <1..>726
                     /locus_tag="OO335_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6004480.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFL
                     KDAIRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGKYWHVYMSKK
                     TAGGRNTARYLGRYLKKPPIAAS"
BASE COUNT          165 a          188 c          231 g          142 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ctgagcttcc
      481 tgaaagacgc gatacgttcg cggtggatgt ggaatatgcg gcaacggctt ctgaaagcgt
      541 ggtcagaggg gctggcgatg ccggagtcgt tgtcacatat cacgacggaa tcacagagga
      601 gaagcctggt gctgaaagcc ggaggaaaat actggcatgt gtacatgtcg aaaaaaacgg
      661 ccggcgggag gaatacggcg cgctacctgg gtcgttatct gaagaagccg ccgatagcgg
      721 cctccc
//
LOCUS       DAJTSF010000320          707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000320 DAJTSF010000000
VERSION     DAJTSF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.331"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..684
                     /locus_tag="OO335_004382"
     rRNA            <1..684
                     /locus_tag="OO335_004382"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          178 a          167 c          210 g          152 t
ORIGIN      
        1 gagctaacgc gttaagtcga ccgcctgggg agtacggccg caaggttaaa actcaaatga
       61 attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgatgca acgcgaagaa
      121 ccttacctgg tcttgacatc cacggaagtt ttcagagatg agaatgtgcc ttcgggaacc
      181 gtgagacagg tgctgcatgg ctgtcgtcag ctcgtgttgt gaaatgttgg gttaagtccc
      241 gcaacgagcg caacccttat cctttgttgc cagcggtccg gccgggaact caaaggagac
      301 tgccagtgat aaactggagg aaggtgggga tgacgtcaag tcatcatggc ccttacgacc
      361 agggctacac acgtgctaca atggcgcata caaagagaag cgacctcgcg agagcaagcg
      421 gacctcataa agtgcgtcgt agtccggatt ggagtctgca actcgactcc atgaagtcgg
      481 aatcgctagt aatcgtggat cagaatgcca cggtgaatac gttcccgggc cttgtacaca
      541 ccgcccgtca caccatggga gtgggttgca aaagaagtag gtagcttaac cttcgggagg
      601 gcgcttacca ctttgtgatt catgactggg gtgaagtcgt aacaaggtaa ccgtagggga
      661 acctgcggtt ggatcacctc cttaccttaa agaagcgttc tttgtag
//
LOCUS       DAJTSF010000321          704 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000321 DAJTSF010000000
VERSION     DAJTSF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.332"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..443)
                     /locus_tag="OO335_004383"
     CDS             complement(<1..443)
                     /locus_tag="OO335_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR6004481.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          214 a          171 c          131 g          188 t
ORIGIN      
        1 tcatctggat aatcggcatg agcaataaca tgcttgccgg tgcgactgtg tacttcaaga
       61 ataaagggca aatgctgaca tttttccagc gccgttttcg cttgtttctg ttgattatct
      121 gccagcgcaa taaaccagtc gccgccattc atcaaccaca aagacatctg ctggaatgcc
      181 agcgcatcca tcgccatctg ttcatgattg cctcttaccg cacgaaccca atgttgttcc
      241 agtaactgca gacaacgtaa actttgcggc ccacgatcga taacgtctcc cactgagata
      301 agtaaatctc gccacggatc aaaacgacaa tgccataatt tgcggcgcaa ctgctcaaga
      361 caaccgtgta tatcgccaga aagccagata tgtcgccatt gatgacccgc aattctctga
      421 taaacgggcg caggctgttt catcaatatt ttcctcccgc gctaaagatc acataatctt
      481 aacaagaatg ttaaaaaacg ctggactcag acagtagagt gtgtgttatg gttgactata
      541 aagtcagcga aggaaatgct tctggctttt aacagataaa aagagaccga acatgattcc
      601 tgttttcgtc aacaaacaat aaaacccttt aaaattaatg cgttgaataa atttacgttc
      661 atcttttaat ccctgacacg tatcattgca tattaataca ttca
//
LOCUS       DAJTSF010000322          694 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000322 DAJTSF010000000
VERSION     DAJTSF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.333"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>694
                     /locus_tag="OO335_004384"
     CDS             <1..>694
                     /locus_tag="OO335_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6004482.1"
                     /translation="RNAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQT
                     TQASNRIRGLLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCK
                     LAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQR
                     VLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRG
                     EHPSRRGNKALKR"
BASE COUNT          139 a          238 c          183 g          134 t
ORIGIN      
        1 tcgtaacgct gccatcattg ccgaagctgc ccgtaccctg cctcacgcgc tacgcacgct
       61 gaaactggct gacgagcaaa tcgccgaact ctccatgctc tgcggcttcg atgatgatct
      121 tgccgcacag acaacgcagg ccagcaaccg tatccgcggc cttctgaccc agatacatcc
      181 ggcaccggag cgcgttctcg gtccgagact tgagcacccg gcggtactcg atcttctcca
      241 gcgatatccc tcaccagaaa aactcgcttc gctgggtgag aagaagctgg cagcccagct
      301 ctgcaaactt gcgcctcgtc tgggtaaacg ccttgcagca gacatagctc aggcactggc
      361 cgaacaaacc gtcgtcgttc ccggcacgaa tgccgctgcc gtagtactgc cacgtctggc
      421 actccagctc atcacgctgc gtaagcaaag agacgaggtg gcgcttgagg tagaacagcg
      481 agttcttgct caccctcttt acccggtcct gaccagtatg cccggagtcg gtgtcaggac
      541 cgcagccaga ctcctcaccg aggtcgcctg ccgcgccttc gcctctgccg cacatctcgc
      601 tgcttatgct ggccttgcgc cggtaactcg gcgatccggc tcgtcaatac gcggtgagca
      661 tccctcgcga cggggtaata aagctctcaa acgg
//
LOCUS       DAJTSF010000323          691 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000323 DAJTSF010000000
VERSION     DAJTSF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.334"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(135..265)
                     /locus_tag="OO335_004385"
                     /pseudo
     CDS             complement(135..265)
                     /locus_tag="OO335_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(263..358)
                     /locus_tag="OO335_004386"
                     /pseudo
     CDS             complement(263..358)
                     /locus_tag="OO335_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          200 a          125 c          166 g          200 t
ORIGIN      
        1 caagggaata tagtttgtgt aagcatatta cttccttatg ttatattttg gtaaaaaatt
       61 tattttttta cattatagtc ggctaaatga agtttactgt aaaaaaacga tactttttcg
      121 ggtggcgctt ccaatcagta gaatatgtgt ttttcaatga aggagccgat gacttttcgt
      181 ggatttccac ggaacgcgag aagcagattg ttttacgtgc cagccgcttg atgcgggtgc
      241 ggagggtcag gttgttacgt tcaagatata gcatgtgctg gatgaagatc ccttttctgg
      301 tcacctgttt atctttcgtg gccgacgggg tgacactgtt aaaatgtaat gaacgcatcc
      361 cacacaatta aacacggagt agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
      421 ctcgcagctt tccggcatca tactgcccaa tacgggatgc ataaagcgtc ctcagactgg
      481 agactccaca aatgaaatat acaccggttg gcgttgatat ggcaaaacat gtcattcaga
      541 ttcacttcat caatgagcac atgtcagcgc cagtgatata agacggtaat ggggatgcgg
      601 acgatttcct ggactctcag aagatgaaac atgtttactg aagaacaaaa aattcgcgct
      661 attgagcttt atttcaaata tggtaagaag c
//
LOCUS       DAJTSF010000324          685 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000324 DAJTSF010000000
VERSION     DAJTSF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.335"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            52..685
                     /locus_tag="OO335_004387"
                     /pseudo
     CDS             52..685
                     /locus_tag="OO335_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          205 a          172 c          173 g          135 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttga
//
LOCUS       DAJTSF010000325          673 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000325 DAJTSF010000000
VERSION     DAJTSF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.336"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..560
                     /locus_tag="OO335_004388"
     CDS             <1..560
                     /locus_tag="OO335_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
                     acyltransferase"
                     /protein_id="HCR6004483.1"
                     /translation="NVIFLVPHGWSVDIPAMLLAAQGKKMAAMFHQQRNPVIDYVWNS
                     VRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKATLPII
                     GRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTIARQMNKTVEILVG
                     PHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          204 a          136 c          157 g          176 t
ORIGIN      
        1 gaaatgtgat ttttctggtt ccccatggct ggagcgtgga tattcctgca atgttgctgg
       61 cagctcaggg aaaaaaaatg gccgccatgt ttcatcagca acgaaatcca gtgattgatt
      121 atgtctggaa ttcagtacgg cgtaaattcg gggggcgctt acatgcccgg gaggatggga
      181 taaaaccatt tattcagtca gtacgccagg gatactgggg atattacctc ccagatcagg
      241 atcatggtcc tgaatacagt gaatttgctg atttttttgc gacctataaa gcgacattac
      301 caatcattgg acgtctgatg aacatcagtc aggctatgat tataccactt ttcccggttt
      361 atgatgaaaa aaaacatcta ctgactattg aaattcggcc accaatggat gcttgcattg
      421 ccagcgcgga caataaaacg attgcccgac aaatgaacaa aacagtggaa attttggtgg
      481 ggccacatcc ggaacagtat gtctgggttt taaaattgtt aaaaacacgc aaatcaaacg
      541 aagcggaccc gtacccttga aattataaag tacaagtatt tccactagtt gtttgtaagc
      601 gtacagcctg aaccgtctgg tcagaatctg acgaattaga caaagtggtg tccaccaaat
      661 aagtagtggg aac
//
LOCUS       DAJTSF010000326          661 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000326 DAJTSF010000000
VERSION     DAJTSF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.337"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            104..>661
                     /locus_tag="OO335_004389"
     CDS             104..>661
                     /locus_tag="OO335_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6004484.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSI"
BASE COUNT          237 a          122 c          110 g          192 t
ORIGIN      
        1 aagcaatacc tggagaaaga gtacaaagca aactgtatta aacttaataa gttatattgt
       61 atttcactag agttcttatc gttgataaga aattctggtt ataatgatta aatcaacgaa
      121 tatacaggta atcggttctg gtattatgca tcaaataaac aacatacact cgttaactct
      181 attttctttg cctgtgagtt tgagtccatc ttgtaatgaa tattatttaa aagtctggag
      241 tgaatgggaa aagaacggta ccccaggcga gcaacgcaat atcgccttca ataggctaaa
      301 aatatgtttg caaaatcaag gggcggaatt aaatctatct gagttagatt taaaaacatt
      361 acctgattta ccgcctcaga taacaacact ggaaataaga aaaaacctat taacatatct
      421 ccctgatttc ccaccaatgc ttaaggtaat acatgctcaa tttaatcaac tggaaagctt
      481 acctgcctta cccgagacgt tagaagagct taatgtgggt gataacaaga taaaagaatt
      541 accatatctt cctgaaactc taactcattt acggatccat aataaccgat tgcatattct
      601 gccactattg ccaccggagc taaaattact gatagtttct ggaaacagat tagacagcat
      661 c
//
LOCUS       DAJTSF010000327          656 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000327 DAJTSF010000000
VERSION     DAJTSF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.338"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(151..648)
                     /locus_tag="OO335_004390"
     CDS             complement(151..648)
                     /locus_tag="OO335_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6004485.1"
                     /translation="MTTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDLKE
                     LRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDPST
                     PVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVE
                     RLMQL"
BASE COUNT          173 a          160 c          143 g          180 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc ttaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DAJTSF010000328          639 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000328 DAJTSF010000000
VERSION     DAJTSF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.339"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..525
                     /locus_tag="OO335_004391"
                     /pseudo
     CDS             1..525
                     /locus_tag="OO335_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          184 a          129 c          153 g          173 t
ORIGIN      
        1 tgtgctgatc gtgcggatga ttactaccat gaccgtctgg cgctgactgt tgataattca
       61 acgatcgacg acaactacga acactacaca tataacggca cttacaataa tgccgctgat
      121 actcatgttg tagacgttta caacattggt acggctatta ctctggatca ggaagttgat
      181 ctgtccatca ctaataactc tcacgttgcg ggtattacgt tgactcaggg ttatgagtgg
      241 gaagatattg acgacaacac tgtcagcact ggcgtaaaca gcagcgaagt gtttaataac
      301 accatcactg ttaaagattc taccgtaacc tctggttcat ggactgatga aggtactact
      361 ggttggttcg gtaatacagg taatgccagc gattatagtg gtaaatctaa ctttgtcacc
      421 gtagatactg acggagatgg tgttgctgat agcactattg caagctggga tgatgttgca
      481 ttagctgtag ttgcacatcc gaatgttgat aatgctatgc agacctgaat cgccacgggt
      541 ttaacagaca cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag
      601 cgttatcccg aagagtttaa aactgaagca gtcaaacag
//
LOCUS       DAJTSF010000329          637 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000329 DAJTSF010000000
VERSION     DAJTSF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.340"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            487..>637
                     /locus_tag="OO335_004392"
     CDS             487..>637
                     /locus_tag="OO335_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004486.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNVLRVTNSSS
                     SGISEK"
BASE COUNT          233 a          115 c          128 g          161 t
ORIGIN      
        1 atcccgtact ctctggccag ttgggccacg caggccccta gctggtaaga tttttcaata
       61 agatcaagct taaactccat agagtcatag agtaagaggc acgaatcctg cgggggcgaa
      121 gagaatctga cattgataga gtccattaac gttataagag gactctatca tcgccaatca
      181 tcagacattg aaatagacga cactgagatg acgcttacaa ttcttttggt ttctgtaata
      241 agtaatctga catcccccgt gcccagaaga ggagagggat atcaagccca agggttaata
      301 attgatacta cggttaaaat tgccttcagt aaaggaaacg tgattaaatt caggggattt
      361 cagagtgaaa accatacaac atattaaaat ctgttcattt agctataata catacaagaa
      421 agtgtccagt tagataatgt tatctaaata accacagata aaaacgcaca taattggggg
      481 ggggggatga aaatacctga agcagtaaat catattaatg ttcagaataa tattgatctt
      541 gttgatggaa aaacgaatcc gaataaagca acaaaagcat tacaaaaaaa tgtattacgc
      601 gtaacaaact catcttcttc tggcataagt gaaaaac
//
LOCUS       DAJTSF010000330          635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000330 DAJTSF010000000
VERSION     DAJTSF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.341"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            18..491
                     /locus_tag="OO335_004393"
     CDS             18..491
                     /locus_tag="OO335_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6004487.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          210 a          113 c          127 g          185 t
ORIGIN      
        1 gagatgaacc ctatgcaatg tcctggttaa tatctcagtg tgctcatcag tgcactgata
       61 ataaaaagac ggagacagat gccatttacg ataaagttcg gagttcttat ttgctttcat
      121 gtattctgaa aaaaaacaaa aatgttgggc ttattcttca tgcaccatca tttgtttctg
      181 tgtctgaaaa aatcgccaga attgtgatgg caaattactc ccgtaactgg agtaattccg
      241 agttggccag tgcggtttta atgagtgaat cttctttaaa aagaagaatg tacaaagagg
      301 tcggttctat aagtacattt gtacataaaa taaagcttac agaagctatc aggaaattac
      361 gaaggacaaa tactcccatt tctgtcatat catctgagtt aggttacagt tcaccatcgt
      421 atttttctaa agtcttcttt aaatatttaa agacataccc tcaaaacata aggaagaaaa
      481 atggtcggta atgtcaccaa ttaggaggac ttgcagagcc gcttttttac gatactgaga
      541 tcaaacagac ataatctatg cggagattta cagcagggca tggttcacaa atctgacagc
      601 gatgaattat cggcactgcg ggcagaaaat gctcg
//
LOCUS       DAJTSF010000331          632 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000331 DAJTSF010000000
VERSION     DAJTSF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.342"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>632)
                     /locus_tag="OO335_004394"
     CDS             complement(<1..>632)
                     /locus_tag="OO335_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HCR6004488.1"
                     /translation="AGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDIPSQVFGWETAIP
                     FLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCGFFLYPLKFSFIR
                     PEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVRWRKVCGGWFLLI
                     AISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIKIALP"
BASE COUNT          211 a          153 c          150 g          118 t
ORIGIN      
        1 atggcagtgc tattttgatt cgacgttgat caagtttctg ataattccaa cgacggtcga
       61 cgggcaccat ccagtcaatc aacataccta ccgccagccc tgtgatgaca tcaataaaat
      121 gatgctgcca tgtcgtcagc gtcgaaatgg cgatgagtaa aaaccatccg ccacagactt
      181 tacgccacct cacagccaga tgctgacgaa agtgacgcca aagtagccag cagagaataa
      241 tatgcagcga aggagactgg ttataaggca gatcaaacag ttcaagttgc gaaaatagcc
      301 atcctgtcac cccactcact tcaggacgga taaaactaaa cttcagcggg tagagaaaaa
      361 aaccgcagca ggccattacc gttgccagaa taagccggtg gacaagtcgg cgttgttcga
      421 atgtcgagct acaaacgaac agcgaaaatc catataaaag atccagactc cagtaaggaa
      481 caatagtcca gggaagaaaa gggatcgccg tttcccaacc gaatacctga ctggggatat
      541 catggctgtt aaggtcctga accgcggtga actgattaag agatccatag gtgaagaaga
      601 aaaacggggc cagcaacaat aaccagccag cg
//
LOCUS       DAJTSF010000332          627 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000332 DAJTSF010000000
VERSION     DAJTSF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.343"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(187..>627)
                     /locus_tag="OO335_004395"
     CDS             complement(187..>627)
                     /locus_tag="OO335_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6004489.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          164 a          174 c          105 g          184 t
ORIGIN      
        1 ggcagaataa taaccatatg tcatcacatc tgcaaactca tctgatcacc agcacgttta
       61 acgtcctgtg ccgtttttca ggtataaaaa aaaccgcata aagcgggctc tttcaaatgt
      121 ccatgtctgc tattcgcctc gcggtacagc tttgcgaagc gtacaagaat tgaagcagtt
      181 tttacgtcaa aaagcaataa cttttttctc tataccaaaa gccataacca ttggtttgta
      241 caaaataaat tctgccacct ttagccaatg ctcaatgcgt ctttcacagg ttcttaaact
      301 ccatttcggg tgtgcatcat tcagcagttc agccattttg catttggtca tcccccgccc
      361 ctcataccgt tgccgaagga cgctaatcaa tcctggatgc tctgccagca cctcacttat
      421 gacccgatca atacataacg cctctgcatc agtacaatgc gccagccagc tcttttgctt
      481 accgttaatc atatcccgca aaaaagcctc aagttcagac ttgttcagac ctgctttttt
      541 catcctccgg agcgcctcgt taattgccgt ttttgtcagc tttttagagg tcaacatcag
      601 gttaaacata ttccccgtct taccgcc
//
LOCUS       DAJTSF010000333          624 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000333 DAJTSF010000000
VERSION     DAJTSF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.344"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>624)
                     /locus_tag="OO335_004396"
     CDS             complement(<1..>624)
                     /locus_tag="OO335_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6004490.1"
                     /translation="VWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPE
                     KQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT          140 a          173 c          140 g          171 t
ORIGIN      
        1 gacacttccg agccgttgat aatactggtt ttcatattct gtcggtgaca tctgttcgct
       61 agaaccatgc cgacgcttac tgttataaaa catttcgatg taatcaaaaa tatcactgcg
      121 ggcttcttcc cgcgttccgt agatcttttt ctttatccgt tcacgtttca acaactggaa
      181 aaaactttct gcaaccgcat tatcatggca gttaccgcga cggctcatgc taccctccag
      241 gccgtgtgat ttcaggaacg actgccactc atggcttgtg tactgactgc cctgatccga
      301 atgaaccagc acctgttttt cgggattacg ccgccataca gccatcagca gtgcgttcag
      361 gacaatgtcc tttgtcatcc gggattgcat ggaccagccg ataattttgc gtgagaacag
      421 atcaacaaca acggcaagat acagccagcc ttcgtgggtc ctgatgtagg ttatgtccgt
      481 tacccaacgc tcatcaggag catccggatt gaactgtcgc tggagcctgt tgggtgacac
      541 gatactggcc tcgcctttac gtgcccgcgg gcttcggtat ccgacctgag cctttattcc
      601 gacacgtttc atcagtctcc agac
//
LOCUS       DAJTSF010000334          623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000334 DAJTSF010000000
VERSION     DAJTSF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.345"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..251)
                     /locus_tag="OO335_004397"
     CDS             complement(<1..251)
                     /locus_tag="OO335_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR6004491.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
     gene            complement(272..>623)
                     /locus_tag="OO335_004398"
     CDS             complement(272..>623)
                     /locus_tag="OO335_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6004492.1"
                     /translation="TVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRH
                     RKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNRR
                     LSESVPCMPHKKAG"
BASE COUNT          141 a          145 c          141 g          196 t
ORIGIN      
        1 ctttgcaggt aagtcagtaa tgtttcttgc tgatcgcgcc agacgttctg cacatcaaca
       61 ttttcattac tgagcagtat tttaactttg ctgacctgca cgccgttgtc gatccagcgt
      121 ttgatctcgc ggatccggtc aatatcggca tcgttgaaca gccgatgacc gccgtctgtc
      181 cgttgcggtt tcagcaatcc gtaacgcctc tgccacgcgc gtaacgtgac agggattaaa
      241 ggtaaaccaa ttgattttgc aagtttttat tttaccccgc tttcttatgg ggcatgcatg
      301 ggacactttc agatagtctt ctgttaagaa gttctatctg ttcgtgattg ttgtctttca
      361 tccatgctcc gtaaacattg aataccattt gtgcgtttgt gtggcccatc tggcttgcga
      421 taaaactagg attagctcct gcggcaagtg accagcatgc ataggtatgc ctggattgat
      481 acgattttct gtgtctcaga cctgcgcgtt ttaagatact tgtccatgac tccctgatgg
      541 agtcaacctt atagtgcggt ccggaaaact gccgctgttt tattacctga ggactaaaaa
      601 caaaagtgca tttatgcaca gta
//
LOCUS       DAJTSF010000335          577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000335 DAJTSF010000000
VERSION     DAJTSF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.346"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..98
                     /locus_tag="OO335_004399"
     CDS             <1..98
                     /locus_tag="OO335_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6004493.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            156..512
                     /locus_tag="OO335_004400"
     CDS             156..512
                     /locus_tag="OO335_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004494.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          183 a          110 c          149 g          135 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatca
//
LOCUS       DAJTSF010000336          574 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000336 DAJTSF010000000
VERSION     DAJTSF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.347"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..574
                     /locus_tag="OO335_004401"
                     /pseudo
     CDS             1..574
                     /locus_tag="OO335_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          137 a          141 c          172 g          124 t
ORIGIN      
        1 tgaacttgct gccgccatga agcagattaa agaactccag cgcctgctcg gcaagaaaac
       61 gatggaaaat gaactcctca aagaagccgt tgaatatgga cgggcaaaaa agtggatagc
      121 gcacgcgccc ttattgcccg gggatgggga gtaagcttag tcagccgttg tctccgggtg
      181 tcgcgtgcgc agttgcacgt cattctcaga cgaaccgatg actggatgga tggccgccgc
      241 agtcgtcaca ctgatgatac ggatgtgctt ctccgtatac accatgttat cggagagctg
      301 cccacgtatg gttatcgtcg ggtatgggcg ctgcttcgca gacaggcaga acttgatggt
      361 atgcctgcga tcaatgccaa acgtgtttac cggctcatgc gccagaatgc gctgttgctt
      421 gagcgaaaac ctgctgtacc gccatcgaaa cgggcacata caggcagagt ggccgtgaaa
      481 gaaagcaatc agcgatggtg ctctgacggg ttcgagttct gctgtgataa cggagagaga
      541 ctgcgtgtca cgttcgcgct ggactgctgt gatc
//
LOCUS       DAJTSF010000337          554 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000337 DAJTSF010000000
VERSION     DAJTSF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.348"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            98..460
                     /locus_tag="OO335_004402"
     CDS             98..460
                     /locus_tag="OO335_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6004495.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT          161 a          134 c          143 g          116 t
ORIGIN      
        1 ttacgttgca gaactattac aatgtcatca gttgtgaaca aagcacctgg tcgcgcatac
       61 taggggctat aaatttatct ttatcagaag ccatcacatg aaagaagtcg aaaaaaacga
      121 aatcaagcgt ctcagcgatc gcctggacgc catccgccac cagcaggccg atctgtcgct
      181 ggttgaagcc gcagacaaat atgccgagct ggaaaaagag aaagccacgc tggaagcaga
      241 aattgctcgc ctgcgtgagg ttcatagcca aaaactgagt aaagaagcac agaagctgat
      301 gaagatgccg ttccagcgcg caattaccaa aaaagagcag gctgatatgg gcaagctgaa
      361 gaaaagtgtt cgcggactgg tggttgtgca cccaatgacc gcgctggggc gcgaaatggg
      421 cctggaagag atgactggat tttctaagac tacgttttaa gcccctcaga cgactggttc
      481 gatcgtatca acccgcatct ggcttgcctt agctggtcaa tgcggggact ctcctgtcta
      541 ctcgcctaat tacc
//
LOCUS       DAJTSF010000338          551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000338 DAJTSF010000000
VERSION     DAJTSF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.349"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>551)
                     /locus_tag="OO335_004403"
     CDS             complement(<1..>551)
                     /locus_tag="OO335_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HCR6004496.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIK"
BASE COUNT          134 a          117 c          135 g          165 t
ORIGIN      
        1 atttaatatc aatctgttga aggtgtatta ttacatcagt agcatattcc agactgcctt
       61 cctggacgat ctgatccata acatcctcac gcactttata tcttgtgcat ttcatttcaa
      121 gtgccgtgag tacagccatt actggtgatg gtagctgtgt tgttttcagg ccgtcgatct
      181 gactgatcgc ttgggcgacg gtgttttgcc attctaaaga acaggtgctg aagtcaaatg
      241 tggctgtttt attatcccgt tgcgacacgc tacaggctaa gcgacgtaat tgggtcaatt
      301 tgtctgcttg ctgaacagga actccgggaa agcggcgatg acttaacgtg gtgtgtacaa
      361 cttcgggcgg aaaaacgaca gcaatatctt tcgccagcca aggtaaaaca gcatctgtat
      421 ttttgtcagc cgcacgggtt tctgctacga aagagggttg ttcctgaata atggtggata
      481 agtccgctac cttgagagag tccgttaccg tttgggaagg ttgcaaagaa ttatctttcg
      541 cttgctccac a
//
LOCUS       DAJTSF010000339          550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000339 DAJTSF010000000
VERSION     DAJTSF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.350"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..143)
                     /gene="csgC"
                     /locus_tag="OO335_004404"
     CDS             complement(<1..143)
                     /gene="csgC"
                     /locus_tag="OO335_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR6004497.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLR"
     gene            complement(202..>550)
                     /gene="csgA"
                     /locus_tag="OO335_004405"
     CDS             complement(202..>550)
                     /gene="csgA"
                     /locus_tag="OO335_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR6004498.1"
                     /translation="NNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGAD
                     VGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVT
                     QVGFGNNATAHQY"
BASE COUNT          146 a          127 c          132 g          145 t
ORIGIN      
        1 tcgcgcaggg acaatatttg tactctgcac agacaagatt gagtaagagt aacttcagga
       61 ataatggtgt acatatcccc ttgctgggtc gtattaaagg ttatctggct ggaaagtgcc
      121 gcaaggagta ataacgcatt catattcttc tcccgaaaaa aaacagggct tgcgccctgt
      181 ttctgtaata caaatgatgt attaatactg atgagcggtc gcgttgttac caaagccaac
      241 ctgagtcacg ttgacggagg agttcgatgc agtctggtca actgcagcac cgttgccacc
      301 accgaactgt ttaacagtca tttcagaatt tttgccgttc cactgatcaa gagtggcgct
      361 gttaccgaag ccacgttggg tcagatcgat tgagctgtca tctgagccct gaccaacatc
      421 tgcaccatta ccaccgccat gctgggtaat agtcaagtca gagttacggg catcagtttg
      481 cagagcaagt gcagagttac cgccaccgta ctggtaaatg ttcagctctg aatttgggcc
      541 gctattatta
//
LOCUS       DAJTSF010000340          534 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000340 DAJTSF010000000
VERSION     DAJTSF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.351"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            19..534
                     /locus_tag="OO335_004406"
                     /pseudo
     CDS             19..534
                     /locus_tag="OO335_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          110 a          144 c          153 g          127 t
ORIGIN      
        1 tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact
       61 gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctatct ctgctctcac
      121 tgccgtaaaa catggcaact gcagttcact tacaccgctt ctcaacccgg tacgcaccag
      181 aaaatcattg atatggccat gaatggcgtt ggatgccggg caaccgcccg cattatgggc
      241 gttggcctca acacgatttt ccgccattta aaaaactcag gccgcagtcg gtaacctcgc
      301 gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat ggacgaacag tggggatacg
      361 tcggggctaa atcgcgccag cgctggctgt tttacgcgta tgacaggctc cggaagacgg
      421 ttgttgcgca cgtattcggt gaacgcacta tggcgacgct ggggcgtctt atgagcctgc
      481 tgtcaccctt tgacgtggtg atatggatga cggatggctg gccgctgtat gaat
//
LOCUS       DAJTSF010000341          510 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000341 DAJTSF010000000
VERSION     DAJTSF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.352"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            368..>510
                     /locus_tag="OO335_004407"
     CDS             368..>510
                     /locus_tag="OO335_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6004499.1"
                     /translation="MSQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIRKML
                     EEL"
BASE COUNT          124 a          133 c          113 g          140 t
ORIGIN      
        1 tagggtctct ctataggtct tctctatggt ctctctatag gtctctctag ggtctcctct
       61 aggcattacc cccagaagct aactctatgg ccctctatgg tcctctatgg ccctgtacgt
      121 cattctttag gatgccctgt agtttcgtat agcctgatgc ctagagagac cctagagaga
      181 ccctagagtg gctcctagag agaccctccc agtagctccg cacagctaca tcgtcatcat
      241 catagtattc aatctcaaag tcattctgtt tgcatcctgt tgtcctgctg gtctcccttc
      301 gcttggggac tttgagtagg tcagtggggg tgcgacttca gggtgcaaac aaaaaggaaa
      361 tcaagcgatg tctcaacaac cacaacaaga aatcagcatc attaacctcg accagcttgt
      421 gtccatgacc tcagttgaaa ttgctgagct gacaggcaag gagcaccgaa atgtccttcg
      481 tgatattcgt aagatgctcg aagaacttaa
//
LOCUS       DAJTSF010000342          486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000342 DAJTSF010000000
VERSION     DAJTSF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.353"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..486
                     /locus_tag="OO335_004408"
                     /pseudo
     CDS             1..486
                     /locus_tag="OO335_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          177 a           81 c           96 g          132 t
ORIGIN      
        1 ttgtcgagta ataatgaata tgacctgttt aaaaatagag aaacctcact gtggaaacgc
       61 aaagtaccag aaagcgcagc gctggcaatg acgggaacag tatataaatt atttggtaat
      121 cgcttactca acgcccgcaa tcagttagat aaaaatctcc tcgaaatggc tatgcaaatg
      181 gtttatggca aagatatggt gacaaagtcc ggggatatat tgattgatat acaattaaat
      241 aaggatggca gtctccagag taagcactac acacttgatg taggtgaagt tataaacagt
      301 tataatattg accctctttg tgaatgcttc aagtattaat caaacttact tacatgataa
      361 aaatcctggt gacgttatta gcctgtatgg cactttagat aacattcccc ttgtagttaa
      421 ggaatgcacg ggtaaaaatc atcaaattaa tgttaaagcc aaattgccac aagaacgaag
      481 tgaaaa
//
LOCUS       DAJTSF010000343          479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000343 DAJTSF010000000
VERSION     DAJTSF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.354"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..479
                     /locus_tag="OO335_004409"
                     /pseudo
     CDS             1..479
                     /locus_tag="OO335_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          120 a          116 c          130 g          113 t
ORIGIN      
        1 ccgtaaaatc atcaatggca tgttctgggt gttatgttcc agtgcaccat ggcgcgattt
       61 acccgaacga tatggggcat ggaaaactgt ttataaccgc tttaaccgat ggtcaaagtc
      121 aggcgtgatt aatattattt tcaacaggtt gctttcgcta cttgatgcaa acggctttat
      181 tgactggtct gccaccgcgc tggatggcag taatatccgg gcgctgaaat gtgccgccgg
      241 tgctcaaaaa aacatcccga tatcgacgga gataatgggc cgggtcgctc tcgcggcggt
      301 tttggcacca aaatccatct ggcaacagac ggaagtggcc tcccgttaaa tatcgtgcta
      361 agccccggac aagctcatga aagccagttc gcgcaacgac ttctggacgg gattggcgtt
      421 cagcgtcaga acggcagcat gaaacgccgt ggacatgcgg tactggctga taaagcgta
//
LOCUS       DAJTSF010000344          471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000344 DAJTSF010000000
VERSION     DAJTSF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.355"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..361
                     /locus_tag="OO335_004410"
     CDS             <1..361
                     /locus_tag="OO335_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6004500.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVE
                     GLKKNARDISLLRYMPA"
BASE COUNT          128 a           99 c          161 g           83 t
ORIGIN      
        1 ggaccacaaa acgggagaaa cggcgacgga aacgctgaca cagcgtgagc tggtcgcgag
       61 gctgaaacag cacatcccgg agaagttttt taagatggtg aggtacttcg ggttccttgc
      121 caaccgggtg tgtggagaga agctgccgca ggtgtaccgt gctctgggga tggataaacc
      181 ggaaccagtg gcgaaagtgt gctatgcaca aatggtgaaa cagttcctga gtcgtgaccc
      241 gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc cgggccatcg cgggactgaa
      301 tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg ctgaggtata tgccggcctg
      361 acacaggcta agtgtgcccg ggaatcggtg caagtataaa aaacagcctg ttgcggtata
      421 agaatcgacc atgcagagag agaaaacacc ggagtggagg gaaaagcaga a
//
LOCUS       DAJTSF010000345          465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000345 DAJTSF010000000
VERSION     DAJTSF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.356"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>465
                     /locus_tag="OO335_004411"
     CDS             <1..>465
                     /locus_tag="OO335_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004501.1"
                     /translation="SGSTLISRDNKLVENFIDTLENWRDQKQIISVICKDGIKIDDSI
                     ITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNTG
                     NTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASA"
BASE COUNT          147 a           96 c          102 g          120 t
ORIGIN      
        1 aatccggtag tacccttatc tcacgagata ataagctggt ggagaacttc atcgacactc
       61 ttgaaaactg gcgtgaccag aagcagatta tttcagttat ctgtaaagat ggtattaaga
      121 ttgacgactc cattattaca agctttaaag cctctaaaga tgttggaatt tcaaacggac
      181 tgagaatcca gctaactttt caggaaatta acttcaaagc tattgtaggg caaactgatg
      241 tttcagcagc cactggacgg acttctacca cgaatgatgg cggtgctacc agtaaaaaga
      301 acactggcaa cactacgaca agtcttggaa gccctatgct gacctgtaaa gagcttttta
      361 gctattcggc aagtgagtta tcagatgaag cgttaaaagc ccgtgtaacg tgctctaaga
      421 gcgttagcgt taaaaatggt gagagcacct tcacagcctc agcta
//
LOCUS       DAJTSF010000346          459 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000346 DAJTSF010000000
VERSION     DAJTSF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.357"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>459)
                     /locus_tag="OO335_004412"
     CDS             complement(<1..>459)
                     /locus_tag="OO335_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HCR6004502.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          146 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaa
//
LOCUS       DAJTSF010000347          457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000347 DAJTSF010000000
VERSION     DAJTSF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.358"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(296..>457)
                     /locus_tag="OO335_004413"
     CDS             complement(296..>457)
                     /locus_tag="OO335_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004503.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          146 a          113 c           81 g          117 t
ORIGIN      
        1 agtcacctct gactgagagt ttactcactt agccgcgtgt ccactattgc tgggtaagat
       61 cactcgatag tcacggacaa tactcactcc ctgcttctgg caaatatgag ctgcgagtgc
      121 tgcaaactcg aaacgacgcc cgtaaaaaca aaactaaaaa atataacgtc gatattcaaa
      181 ttaagtaaat gtcaaccgac ggggactatc cccgtcatag acgttaacgc cccactcctc
      241 tctgtcgctc aagaggttaa tgacgaatag cagagaacat aatgtaaaca aggcatcact
      301 cacgacacat aaataacatc tttgtggtgg tttgattgat ttttccactc cgcttaccat
      361 ctacaccaaa ataacgatca tcacgacata atttttcttt aacatcgcaa aatacaccgt
      421 tagcgaatgt aaattcgctg ggatcgtagc cttgtaa
//
LOCUS       DAJTSF010000348          445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000348 DAJTSF010000000
VERSION     DAJTSF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.359"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..373)
                     /locus_tag="OO335_004414"
     CDS             complement(<1..373)
                     /locus_tag="OO335_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004504.1"
                     /translation="MVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFGGM
                     VRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEFDI
                     WNLNETWAFQEKLIFCEDESSL"
BASE COUNT          134 a          106 c           96 g          109 t
ORIGIN      
        1 acagacttga ttcatcctca caaaatataa gtttttcctg gaatgcccat gtctcattga
       61 ggttccagat atcaaattcg aagtcgtgat aacgaaaacg gatcccacca aacttattaa
      121 aacgcagttg tttgacgggc agttcagcca atgtctggaa gagttcctca cgggagcgac
      181 cgatgacgat atcaaggtct gaagagaatc catcgacgcc atacaagcct aagtcacgca
      241 ccatcccgcc aaaaatcctg gccggacagg aacaggccag agtatcaagt aatactctgg
      301 ctttcttatg ctcatcacta agcgtaaaaa aatcggcgat acgctgttca agcagttggg
      361 tgtttgagac cacattcata cagtgatttt agctcatcgt gaaggcgcat taaataacta
      421 tccgtcatgc ctgagattgc gtcgg
//
LOCUS       DAJTSF010000349          445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000349 DAJTSF010000000
VERSION     DAJTSF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.360"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            25..>445
                     /gene="ospC4"
                     /locus_tag="OO335_004415"
     CDS             25..>445
                     /gene="ospC4"
                     /locus_tag="OO335_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR6004505.1"
                     /translation="MTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGK
                     TDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYH
                     IPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTD"
BASE COUNT          159 a           59 c           84 g          143 t
ORIGIN      
        1 gatcatgact ctccatatgg atatatgaca ttaaccgatc actttgataa tgctattcca
       61 cctgtttttt accatgagca ccaatcattt ttcttagata actttaaaga ggttgtggat
      121 gaagttagca ggtatgtgca tggtaatcag gggaaaactg atgttccaat atttaacact
      181 aaagatatga ggcttggaat tgggttgcat cttattgatt ttataagaaa gagtaaagat
      241 caaggattca gagagttttg ttataacaaa aatattgatc ctgttagtct ggatagaatt
      301 ataaactttg tgtttcagct tgagtatcat ataccaagaa tgctaagtac agataacttc
      361 aaaaaaataa aactcagaga catatctttg gaggatgcga ttaaagcatc taattatgaa
      421 gagattaaca ataaagtgac tgata
//
LOCUS       DAJTSF010000350          443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000350 DAJTSF010000000
VERSION     DAJTSF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.361"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            213..>443
                     /locus_tag="OO335_004416"
     CDS             213..>443
                     /locus_tag="OO335_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6004506.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          141 a          105 c           61 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DAJTSF010000351          438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000351 DAJTSF010000000
VERSION     DAJTSF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.362"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..149
                     /locus_tag="OO335_004417"
                     /pseudo
     CDS             1..149
                     /locus_tag="OO335_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(169..>438)
                     /locus_tag="OO335_004418"
     CDS             complement(169..>438)
                     /locus_tag="OO335_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HCR6004507.1"
                     /translation="KSRSTGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQANSIK
                     SRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT          111 a          105 c          102 g          120 t
ORIGIN      
        1 cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct gcaacgtgac gcatatcaga
       61 ccggtcagca ccggttccat ccttcgctgt ggcagttcgg caaggagatg ggcttctctc
      121 cccgactgtg tcgccccttc aggctgaggg atccccacaa aattactgct aataaaccag
      181 caccatattt cggtaggttc tggacagatg gtttagaact gtgctcagta ctgttcgtct
      241 taaaataagt ggcgagtgtc gaagaacatt cttcgctaac gtcagatatg agataacccg
      301 ccgggattta atgctgttag cctgatattt caggtgtaac cctttatttt cagcgtgata
      361 accgagtaac cacattacga tagtgctcaa cgttgccagc agactcagaa ccagaattct
      421 tcctgttgaa cgactttt
//
LOCUS       DAJTSF010000352          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000352 DAJTSF010000000
VERSION     DAJTSF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.363"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            35..434
                     /locus_tag="OO335_004419"
                     /pseudo
     CDS             35..434
                     /locus_tag="OO335_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAJTSF010000353          430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000353 DAJTSF010000000
VERSION     DAJTSF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.364"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..279)
                     /locus_tag="OO335_004420"
     CDS             complement(1..279)
                     /locus_tag="OO335_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004508.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
BASE COUNT           95 a          100 c          134 g          101 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc
//
LOCUS       DAJTSF010000354          415 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000354 DAJTSF010000000
VERSION     DAJTSF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.365"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>415
                     /locus_tag="OO335_004421"
     CDS             <1..>415
                     /locus_tag="OO335_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6004509.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTL"
BASE COUNT           93 a          136 c          112 g           74 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatgagctt gctgagcaac ttatagaaca aaccggcgat aacactctga cgtta
//
LOCUS       DAJTSF010000355          391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000355 DAJTSF010000000
VERSION     DAJTSF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.366"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>391
                     /gene="tuf"
                     /locus_tag="OO335_004422"
     CDS             <1..>391
                     /gene="tuf"
                     /locus_tag="OO335_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6004510.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAI"
BASE COUNT           85 a           99 c          119 g           88 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttatattc cggaaccaga gcgtgcgatt g
//
LOCUS       DAJTSF010000356          388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000356 DAJTSF010000000
VERSION     DAJTSF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.367"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(40..>388)
                     /locus_tag="OO335_004423"
     CDS             complement(40..>388)
                     /locus_tag="OO335_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6004511.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           89 c           87 g          120 t
ORIGIN      
        1 ttcgtgaagc tgttgaaggg tggattctta acggtggcgt taatcagaaa tgctcctgat
       61 gtgccagaac aatttatcag cgtgaagctc atacgctttg cgctgagcct ctatccagtc
      121 gtgtttattg tatacggaca ttatcccgcc aagttcatga ccgagcattc gctcagtaac
      181 atgaggctcg acattcatct cggacagccg tgtaacaagc gttcttctga aatcatgtgt
      241 tcgccagtaa cctagatcca tccctcccct tattctattt acgaatcggt tggctgcagc
      301 gatgctgatc ggtttgttta tgtcttctcc agggaaaaga acatcattgt acattgtcat
      361 ggccttttcg agaaaaggct taatttgt
//
LOCUS       DAJTSF010000357          380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000357 DAJTSF010000000
VERSION     DAJTSF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.368"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(22..98)
                     /locus_tag="OO335_004424"
     tRNA            complement(22..98)
                     /locus_tag="OO335_004424"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(62..64),aa:Lys,seq:ttt)
     gene            complement(100..175)
                     /locus_tag="OO335_004425"
     tRNA            complement(100..175)
                     /locus_tag="OO335_004425"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(140..142),aa:Ile,seq:cat)
BASE COUNT           95 a          102 c           87 g           96 t
ORIGIN      
        1 cctactgagc caagtcggca ctggaccgcc accggggact cgaacctcgc acactcaact
       61 taaagggttg acgctctttc ctgatgagct agtggcggtt ggtggccctt gctggatttg
      121 aaccagcgac ctggcgatta tgagtcgctc gctctcacca ctgagctaaa gggccgggcg
      181 caggataata acgttacgaa atcaatgttg caagcattca aaaatcaccc ttatctcctc
      241 caccagcgca ttcaccatgt ctatccgaga taagtggcac aaaaaacccg cttgtgggcg
      301 ggttttgttt gcttttgcca tcacgtacaa aatcggcaaa gtatcagatt tgcatgaata
      361 tatgcctttc aatctacttt
//
LOCUS       DAJTSF010000358          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000358 DAJTSF010000000
VERSION     DAJTSF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.369"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>375
                     /locus_tag="OO335_004426"
     CDS             <1..>375
                     /locus_tag="OO335_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6004512.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAJTSF010000359          365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000359 DAJTSF010000000
VERSION     DAJTSF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.370"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            32..>365
                     /locus_tag="OO335_004427"
     CDS             32..>365
                     /locus_tag="OO335_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR6004513.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDME"
BASE COUNT          117 a           78 c           74 g           96 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaa
//
LOCUS       DAJTSF010000360          348 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000360 DAJTSF010000000
VERSION     DAJTSF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.371"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>348
                     /gene="torD"
                     /locus_tag="OO335_004428"
     CDS             <1..>348
                     /gene="torD"
                     /locus_tag="OO335_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6004514.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSF"
BASE COUNT           83 a           82 c          102 g           81 t
ORIGIN      
        1 acgctgacag tacgtgacga tgcccgtctg gaactggccg ctgacttttg cggcctgttt
       61 ctgatgaccg acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa
      121 gagattaaac gcttgttagt tgaggcaggg atggaaacca gtggcaattt caacgaaccg
      181 gcagatcatc tggcgatcta tctcgagttg ttgagttatc tgcatttttc gctgggagag
      241 gggaccgttc ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac ggcgctgcgg
      301 caatggttac cagagtttgc tgcgcgttgt cgacagtatg acagcttt
//
LOCUS       DAJTSF010000361          340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000361 DAJTSF010000000
VERSION     DAJTSF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.372"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>340
                     /locus_tag="OO335_004429"
     CDS             <1..>340
                     /locus_tag="OO335_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6004515.1"
                     /translation="YLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGV
                     MFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYV
                     SFSEAAHAIT"
BASE COUNT           82 a           85 c          108 g           65 t
ORIGIN      
        1 cgtacctcgc cgttgttctc gacctgttcg caagaaaacc agtgggctgg gccatgtcgt
       61 tctcgccgga cagcaggctc accatgaaag cgctggaaat ggcatgggaa acccgtggta
      121 agcccggcgg ggtgatgttc cacagcgatc agggcagtca ttatacgagc aggcagttcc
      181 ggcagttatt gtggcgatac cagatcagac agagtatgag ccggcgcgga aactgctggg
      241 ataacagccc aatggaacgc ttcttcagga gtctgaagaa cgaatggatg ccggtggtgg
      301 gttacgtaag cttcagcgag gcagctcacg ccataacgga
//
LOCUS       DAJTSF010000362          340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000362 DAJTSF010000000
VERSION     DAJTSF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.373"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            4..171
                     /gene="xisR"
                     /locus_tag="OO335_004430"
     CDS             4..171
                     /gene="xisR"
                     /locus_tag="OO335_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014884030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR6004516.1"
                     /translation="MLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWYN
                     YPKINQFIKDS"
     gene            173..>340
                     /locus_tag="OO335_004431"
     CDS             173..>340
                     /locus_tag="OO335_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HCR6004517.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          108 a           64 c           82 g           86 t
ORIGIN      
        1 ctgatgcttc gcactggtct gggtgccaga caaattgaag cataccgcca gaactgttgg
       61 gtggaaggct tccacttcaa acgagtatct cctttaggga agccagacag taagcgaggg
      121 attatctggt acaactatcc gaagataaat cagtttatca aagactcatg atatgtctaa
      181 attaccaaca ggtgtcgaga ttcgaggtaa atacattcgc atctggttca tgtttcgagg
      241 aaaacgatgt cgggaaacat tgaaaggctg ggagattact aacagtaaca ttaaaaaggc
      301 cgggaattta agagcgttga tagttcatca aatcagttcc
//
LOCUS       DAJTSF010000363          335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000363 DAJTSF010000000
VERSION     DAJTSF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.374"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>335
                     /locus_tag="OO335_004432"
     CDS             <1..>335
                     /locus_tag="OO335_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6004518.1"
                     /translation="NGKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVP
                     IALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPG
                     TETSNIQLA"
BASE COUNT           92 a           79 c           78 g           86 t
ORIGIN      
        1 taatggtaaa gttattgctc cagcttgtac cctggtagct gcgacgaaag attcagtagt
       61 cacattgcca aatgtcagcg ccaccaagtt gcaaactaat ggagcagtct ctggcgttaa
      121 aactgatgta ccaattgcct tagaaggctg tgatgttact gtaactaaaa atgcgacgtt
      181 tacctttagt ggtacggctg acggcgtgca acctacagca tttgccaacc aggctaccac
      241 cgatgcagcg acgaatgttg ctctacaaat gtatctgcca gatggttcta catcggtaac
      301 tcccggtacc gaaacgtcga atattcagtt agcgg
//
LOCUS       DAJTSF010000364          332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000364 DAJTSF010000000
VERSION     DAJTSF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.375"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            51..>332
                     /locus_tag="OO335_004433"
     CDS             51..>332
                     /locus_tag="OO335_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6004519.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT           83 a           70 c           98 g           81 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg ac
//
LOCUS       DAJTSF010000365          331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000365 DAJTSF010000000
VERSION     DAJTSF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.376"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(43..330)
                     /locus_tag="OO335_004434"
     CDS             complement(43..330)
                     /locus_tag="OO335_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004520.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           91 a           85 c           66 g           89 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgca tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt cttcatccat c
//
LOCUS       DAJTSF010000366          325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000366 DAJTSF010000000
VERSION     DAJTSF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.377"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT           70 a           92 c           79 g           84 t
ORIGIN      
        1 gattcaggca ccagaatcac ccgacgcccc ggaggcgctg ccggggtgtc gttccatttc
       61 tgatgatgac ctgtgcgcct ggtgtacccg tctgctttac cgtcccggag aaatcagtct
      121 gtgcagactg agtatacagg caatggcatc tggccatccg tctgtgaccg gaacggttac
      181 gcgtacggct gtccggaatt tcagccgaat atcattcatc cctgatatta ccgggtcatc
      241 acggtcagtg atgaacgtga tgttgccagc tcccgacctt tactccctct catttattca
      301 cattacaagg atttcataca tgaaa
//
LOCUS       DAJTSF010000367          323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000367 DAJTSF010000000
VERSION     DAJTSF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.378"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            103..>323
                     /locus_tag="OO335_004435"
     CDS             103..>323
                     /locus_tag="OO335_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004521.1"
                     /translation="MIYSNQLRYTGKDGVYFHLQGQPDNFLSLSATENMEFDSPMMVT
                     TQNMQSGQTVTDNVMRSPRTISISGVVVVG"
BASE COUNT          109 a           56 c           61 g           97 t
ORIGIN      
        1 aatttacgta gctcacgctt aacagagaaa atacttatat atcaaaggct tggttaaaaa
       61 tgattaaaat gcgctaagtc tttggaatca aaagagaatt taatgattta ctctaatcag
      121 cttcgttaca ctggcaaaga tggtgtttac ttccatctac aaggccaacc agataatttc
      181 ttgtcactat cagcaactga aaacatggaa tttgatagcc ctatgatggt taccactcaa
      241 aatatgcaat cagggcagac tgtaacagat aatgtaatgc gttcacctag aactatttca
      301 atcagtggtg tggttgtggt tgg
//
LOCUS       DAJTSF010000368          306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000368 DAJTSF010000000
VERSION     DAJTSF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.denovo.379"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>306
                     /locus_tag="OO335_004436"
     CDS             <1..>306
                     /locus_tag="OO335_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6004522.1"
                     /translation="ICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQA
                     VKAELFGGKGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRS
                     "
BASE COUNT           97 a           53 c           80 g           76 t
ORIGIN      
        1 tatttgttgg gaatatcaga tcgcgataac acagtgggcg tggaatgaat ttaatgctca
       61 atccggtacc agaaaaattg cagggaaaac gcaggaacgc ctgaaaaaat taatctggct
      121 ggcggcgcag gcagtaaaag cagaactttt tggtgggaaa ggttatgaat accaggagct
      181 ggcattactg gcgggagtga caactaaaaa ctggtccaaa acatttactc gtcactgggt
      241 tgcaatgaaa cacatttttc accgactgga tagtgaggct ttattgtttg taatgagaac
      301 acgttc
//
LOCUS       DAJTSF010000369         7387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.guided.155, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000369 DAJTSF010000000
VERSION     DAJTSF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.guided.155"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(16..204)
                     /locus_tag="OO335_004437"
     CDS             complement(16..204)
                     /locus_tag="OO335_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6004523.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(423..1400)
                     /gene="epmA"
                     /locus_tag="OO335_004438"
     CDS             complement(423..1400)
                     /gene="epmA"
                     /locus_tag="OO335_004438"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6004524.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1725..3533
                     /gene="frdA"
                     /locus_tag="OO335_004439"
     CDS             1725..3533
                     /gene="frdA"
                     /locus_tag="OO335_004439"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6004525.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3526..4260
                     /gene="frdB"
                     /locus_tag="OO335_004440"
     CDS             3526..4260
                     /gene="frdB"
                     /locus_tag="OO335_004440"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6004526.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4271..4666
                     /gene="frdC"
                     /locus_tag="OO335_004441"
     CDS             4271..4666
                     /gene="frdC"
                     /locus_tag="OO335_004441"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6004527.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4677..5036
                     /gene="frdD"
                     /locus_tag="OO335_004442"
     CDS             4677..5036
                     /gene="frdD"
                     /locus_tag="OO335_004442"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6004528.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5099..6232
                     /locus_tag="OO335_004443"
     CDS             5099..6232
                     /locus_tag="OO335_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6004529.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6321..6854
                     /gene="blc"
                     /locus_tag="OO335_004444"
     CDS             6321..6854
                     /gene="blc"
                     /locus_tag="OO335_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6004530.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="OO335_004445"
     CDS             complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="OO335_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6004531.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
BASE COUNT         1866 a         1918 c         1903 g         1700 t
ORIGIN      
        1 gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg gaataaagaa
       61 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
      121 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
      181 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
      241 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
      301 tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
      361 atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
      421 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
      481 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accgactttc
      541 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
      601 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
      661 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
      721 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
      781 tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
      841 tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc ccgcagttgc
      901 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
      961 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
     1021 aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc agggttgtga
     1081 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaacca
     1141 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     1201 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     1261 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
     1321 cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc gctcggctgc
     1381 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     1441 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
     1501 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     1561 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     1621 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     1681 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     1741 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     1801 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     1861 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     1921 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     1981 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc ccatggagcc
     2041 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     2101 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     2161 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     2221 gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg cagatccgtg
     2281 ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     2341 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     2401 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
     2461 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     2521 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     2581 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct
     2641 ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag aaaaaactgc
     2701 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     2761 aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc gaaaccgatc
     2821 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     2881 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc
     2941 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
     3001 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac caggatggcg
     3061 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
     3121 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
     3181 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
     3241 ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     3301 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     3361 tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctggagt
     3421 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
     3481 atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc tgagatgaaa
     3541 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     3601 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa
     3661 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
     3721 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     3781 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     3841 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     3901 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
     3961 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     4021 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     4081 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt atggagctgt
     4141 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     4201 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     4261 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     4321 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     4381 gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc cggaagcctg
     4441 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     4501 gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     4561 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     4621 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     4681 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     4741 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     4801 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     4861 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     4921 gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct
     4981 acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca atctaacgca
     5041 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     5101 gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat ttgctgcccc
     5161 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     5221 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     5281 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     5341 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     5401 aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac
     5461 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     5521 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     5581 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     5641 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     5701 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     5761 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     5821 gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6361 ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta gtgaataatt
     6421 tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     6481 aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg
     6541 tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt
     6601 actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     6661 gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg
     6721 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     6781 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     6841 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     6901 gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     6961 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     7021 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata acactcggcg
     7081 tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     7141 cagcaataac taagataatc caggacatat caggctccgg aaatttgggg ccgtccccgg
     7201 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     7261 gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca aacggcacga
     7321 cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc agaaatcgcg
     7381 ttaccgc
//
LOCUS       DAJTSF010000370         5962 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.guided.171, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000370 DAJTSF010000000
VERSION     DAJTSF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.guided.171"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OO335_004446"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OO335_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6004532.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1596..2399
                     /locus_tag="OO335_004447"
                     /note="aph(3'')-Ib"
     CDS             1596..2399
                     /locus_tag="OO335_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR6004533.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2399..3235
                     /locus_tag="OO335_004448"
     CDS             2399..3235
                     /locus_tag="OO335_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR6004534.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVSLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3342..3746)
                     /locus_tag="OO335_004449"
                     /pseudo
     CDS             complement(3342..3746)
                     /locus_tag="OO335_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3790..4179)
                     /locus_tag="OO335_004450"
     CDS             complement(3790..4179)
                     /locus_tag="OO335_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004535.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4397..4837
                     /locus_tag="OO335_004451"
     CDS             4397..4837
                     /locus_tag="OO335_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6004536.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(5582..5731)
                     /locus_tag="OO335_004452"
     CDS             complement(5582..5731)
                     /locus_tag="OO335_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004537.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1453 a         1569 c         1551 g         1389 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggttttttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
     1621 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
     1681 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
     1741 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
     1801 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
     1861 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
     1921 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
     1981 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
     2041 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
     2101 tgcggctcga ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga
     2161 acttcatggt ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg
     2221 gaacagcaga tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg
     2281 cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag
     2341 cccccgaccg cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat
     2401 gttcatgccg cctgtttttc ctgctcattg gcacgtttcg caacctgttc tcattgcgga
     2461 caccttttcc agcctcgttt ggaaagtttc attgccagac gggactcctg caatcgtcaa
     2521 gggattgaaa cctatagaag acattgctga tgaactgcgc ggggccgact atctggtatg
     2581 gcgcaatggg aggggagcag tccggttgct cggtcgtgag aacaatctga tgttgctcga
     2641 atatgccggg gagcgaatgc tctctcacat cgttgccgag cacggcgact accaggcgac
     2701 cgaaattgca gcggaactaa tggcgaagct gtatgccgca tctgaggaac ccctgccttc
     2761 tgcccttctc ccgatccggg atcgctttgc agctttgttt cagcgggcgc gcgatgatca
     2821 aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt atagccgatc aaatgatgag
     2881 caatgcctcg gaactgcgtg ggctacatgg cgatctgcat catgaaaaca tcatgttctc
     2941 cagtcgcggc tggctggtga tagatcccgt cagtctggtc ggtgaagtgg gctttggcgc
     3001 cgccaatatg ttctacgatc cggctgacag agacgacctt tgtctcgatc ctagacgcat
     3061 tgcacagatg gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga
     3121 ccaggcgtac gcttatgggt gcctttccgc agcttggaat gcggatggag aagaggagca
     3181 acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga cagacgtcat actagatatc
     3241 aagcgacttc tcctatcccc tgggaacaca tcaatctcac cggagaatat cgctggccaa
     3301 agccttagcg taggattccg ccccttcccg caaacgaccc caaacaggaa acgcagctga
     3361 aacgggaagc tcaacaccca ctgacgcatg ggttgttcag gcagtacttc atcaaccagc
     3421 aaggcggcac tttcggccat ccgccgcgcc ccacagctcg ggcagaaacc gcgacgctta
     3481 cagctgaaag cgaccaggtg ctcggcgtgg caagactcgc agcgaacccg tagaaagcca
     3541 tgctccagcc gcccgcattg gagaaattct tcaaattccc gttgcacata gcccggcaat
     3601 tcctttccct gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga
     3661 tagagaaggg tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg
     3721 gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca
     3781 gtctatcgct tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca
     3841 ttgccagcca gtgcccgtca ctcccactct tacattgttc gcagtatcta ggtacatgtt
     3901 attgcgtgca ccttcatgat gtataatgtt attaatgaca gcagaaagat cgacagaatt
     3961 aatattcttc gtacctggga cagttatcgg gtgtcggcca gtgatgagac caaaggatgc
     4021 agtataagat gaaccactac tattatcatg gccaccatta ccaccaccgt taaaactacc
     4081 tcccgaactt ccagagccgc tatgaccatc attgtagggg tcatttggtc ccatgtgacc
     4141 aaaaccaccg acagtaccgc tatttaactc tgtactcata aaattatcct ctttgacttt
     4201 taaaaacagc aaaaactgta tttataacaa tgtgtttata tacagtattc gaaatactac
     4261 gcccgtcaaa accacaccgt caacaggttt tttgtgtatt tttcacgcct cacgggcagc
     4321 ctcagacctt ttgtgttaaa acagtaatat gttctgctgt tacaatacgg tacacacagg
     4381 agacactgtc acttttatgg catggcacac agttcaagag acactgaaat taacaggaaa
     4441 gtcgcgctcg caattatatc gtgacatggc taaaggcctt gtcagctatc gcacaggaaa
     4501 aaatgatcgc cgggagttcg agaccagtga actgattcgg gcctatggcg agctgcgcac
     4561 ttctgagaca ccaaaaaggc acaaggaggg acactccaaa tctgtcgtta gccaggcgga
     4621 aaatgctcag ctagacgtca ttcagcagaa actggacgct ctgcagcaga ctgttaccct
     4681 aatgcttgaa gacaagatgg cgcgggaagc agaaaaccgc ctgcgaaatg aggagaggga
     4741 acaactacag gccgaaatta gccgtctggc tgacgcgctg gcacaggaaa aaaagcgggg
     4801 attctggtcg cggctgtttc gctccggttc agagtaacag tgcaggtgac tctgatttac
     4861 cataacatct gttatcagca ccgcctgaga ggccctcagg gcgtttctga cgggaaaccg
     4921 gtaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg
     4981 gcatgcatga gtatgcgcac aagtgcatag cgcggaaatt cacaccattc agccggggta
     5041 agtcctgttt ccggcgttct tcccatccga aaacggtgaa cggggacgca atttattgcg
     5101 tggccgtgaa gcgttgccgg cgcagccgga aaacccgctg tcagcacgtt caggaagggg
     5161 gtgatgtaca aatggcacag aggatgaaag tgacgtatga gctgaaggaa tgatgtaaaa
     5221 gtgatgtata agtgatgtaa aagtgatgta tgaactgaag gagcgatgta attctgtagt
     5281 caggaagaat aacccgttca tgcaggcatg aacgatacag tgaggaacgc tttttttgtc
     5341 acgatctccc cttaacctgc gtaaatcacc cgccatggtt gattattttt caacctcaaa
     5401 atggtcatac acctaacgca gccgcgattt tcatcgcaac tgcatgaatt aaaagaaaaa
     5461 aaaggaatat ttcgttttca cgagtaatac ccgggtatta aaaatccact gtaattacat
     5521 gaaaataaag aaataaaagg taataccgga gtattaccac gtacgcgggc aagcgaaaaa
     5581 gctataacgc ttcccggccg acaggcgacc caaaatccac aggggcatgc aggttctctt
     5641 cagtaacctg cgccattaaa tcaaaaaggt catattcgcc gggaagctgc gtatcagtcg
     5701 aatccagttg ttcatcatat tcagatggca tggggtgata tccagtagct gagaggttgt
     5761 tagcgggttt cggggcgcag ccctgaacca gtcatgtagc gctagcggag tgtatactgg
     5821 cttactatgt tgcaacgatt gcgaaaatca gtgaagtgct tcatgtggca ggagaaaaaa
     5881 ggcagcaccg gcgcgtcagc agaatatgcg atacaggaga tattccgctt cctcgctcac
     5941 tgactcgcta cgctcggtcg tt
//
LOCUS       DAJTSF010000371         5961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.guided.172, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000371 DAJTSF010000000
VERSION     DAJTSF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.guided.172"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OO335_004453"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OO335_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6004538.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1640..2398
                     /locus_tag="OO335_004454"
     CDS             1640..2398
                     /locus_tag="OO335_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3'') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR6004539.1"
                     /translation="MPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGERDRLIWLK
                     GRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLGAVHSLSV
                     DQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELPVRLDQER
                     TDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENWAAPDE
                     AERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2398..3234
                     /locus_tag="OO335_004455"
     CDS             2398..3234
                     /locus_tag="OO335_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR6004540.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVSLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3341..3745)
                     /locus_tag="OO335_004456"
                     /pseudo
     CDS             complement(3341..3745)
                     /locus_tag="OO335_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3789..4178)
                     /locus_tag="OO335_004457"
     CDS             complement(3789..4178)
                     /locus_tag="OO335_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004541.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4396..4836
                     /locus_tag="OO335_004458"
     CDS             4396..4836
                     /locus_tag="OO335_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6004542.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(5581..5730)
                     /locus_tag="OO335_004459"
     CDS             complement(5581..5730)
                     /locus_tag="OO335_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6004543.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1453 a         1569 c         1551 g         1388 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggttttttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat atttttttgg
     1621 tgaatcgcat tctgactggt tgcctgtcag aggcggagaa tctggtgatt ttgtttttcg
     1681 acgtggtgac gggcatgcct tcgcgaaaat cgcacctgct tcccgccgcg gtgagctcgc
     1741 tggagagcgt gaccgcctca tttggctcaa aggtcgaggt gtggcttgcc ccgaggtcat
     1801 caactggcag gaggaacagg agggtgcatg cttggtgata acggcaattc cgggagtacc
     1861 ggcggctgat ctgtctggag cggatttgct caaagcgtgg ccgtcaatgg ggcagcaact
     1921 tggcgctgtt cacagcctat cggttgatca atgtccgttt gagcgcaggc tgtcgcgaat
     1981 gttcggacgc gccgttgatg tggtgtcccg caatgccgtc aatcccgact tcttaccgga
     2041 cgaggacaag agtacgccgc agctcgatct tttggctcgt gtcgaacgag agctaccggt
     2101 gcggctcgac caagagcgca ccgatatggt tgtttgccat ggtgatccct gcatgccgaa
     2161 cttcatggtg gaccctaaaa ctcttcaatg cacgggtctg atcgaccttg ggcggctcgg
     2221 aacagcagat cgctatgccg atttggcact catgattgct aacgccgaag agaactgggc
     2281 agcgccagat gaagcagagc gcgccttcgc tgtcctattc aatgtattgg ggatcgaagc
     2341 ccccgaccgc gaacgccttg ccttctatct gcgattggac cctctgactt ggggttgatg
     2401 ttcatgccgc ctgtttttcc tgctcattgg cacgtttcgc aacctgttct cattgcggac
     2461 accttttcca gcctcgtttg gaaagtttca ttgccagacg ggactcctgc aatcgtcaag
     2521 ggattgaaac ctatagaaga cattgctgat gaactgcgcg gggccgacta tctggtatgg
     2581 cgcaatggga ggggagcagt ccggttgctc ggtcgtgaga acaatctgat gttgctcgaa
     2641 tatgccgggg agcgaatgct ctctcacatc gttgccgagc acggcgacta ccaggcgacc
     2701 gaaattgcag cggaactaat ggcgaagctg tatgccgcat ctgaggaacc cctgccttct
     2761 gcccttctcc cgatccggga tcgctttgca gctttgtttc agcgggcgcg cgatgatcaa
     2821 aacgcaggtt gtcaaactga ctacgtccac gcggcgatta tagccgatca aatgatgagc
     2881 aatgcctcgg aactgcgtgg gctacatggc gatctgcatc atgaaaacat catgttctcc
     2941 agtcgcggct ggctggtgat agatcccgtc agtctggtcg gtgaagtggg ctttggcgcc
     3001 gccaatatgt tctacgatcc ggctgacaga gacgaccttt gtctcgatcc tagacgcatt
     3061 gcacagatgg cggacgcatt ctctcgtgcg ctggacgtcg atccgcgtcg cctgctcgac
     3121 caggcgtacg cttatgggtg cctttccgca gcttggaatg cggatggaga agaggagcaa
     3181 cgcgatctag ctatcgcggc cgcgatcaag caggtgcgac agacgtcata ctagatatca
     3241 agcgacttct cctatcccct gggaacacat caatctcacc ggagaatatc gctggccaaa
     3301 gccttagcgt aggattccgc cccttcccgc aaacgacccc aaacaggaaa cgcagctgaa
     3361 acgggaagct caacacccac tgacgcatgg gttgttcagg cagtacttca tcaaccagca
     3421 aggcggcact ttcggccatc cgccgcgccc cacagctcgg gcagaaaccg cgacgcttac
     3481 agctgaaagc gaccaggtgc tcggcgtggc aagactcgca gcgaacccgt agaaagccat
     3541 gctccagccg cccgcattgg agaaattctt caaattcccg ttgcacatag cccggcaatt
     3601 cctttccctg ctctgccata agcgcagcga atgccgggta atactcgtca acgatctgat
     3661 agagaagggt ttgctcgggt cggtggctct ggtaacgacc agtatcccga tcccggctgg
     3721 ccgtcctggc cgccacatga ggcatgttcc gcgtccttgc aatactgtgt ttacatacag
     3781 tctatcgctt agcggaaagt tcttttaccc tcagccgaaa tgcctgccgt tgctagacat
     3841 tgccagccag tgcccgtcac tcccactctt acattgttcg cagtatctag gtacatgtta
     3901 ttgcgtgcac cttcatgatg tataatgtta ttaatgacag cagaaagatc gacagaatta
     3961 atattcttcg tacctgggac agttatcggg tgtcggccag tgatgagacc aaaggatgca
     4021 gtataagatg aaccactact attatcatgg ccaccattac caccaccgtt aaaactacct
     4081 cccgaacttc cagagccgct atgaccatca ttgtaggggt catttggtcc catgtgacca
     4141 aaaccaccga cagtaccgct atttaactct gtactcataa aattatcctc tttgactttt
     4201 aaaaacagca aaaactgtat ttataacaat gtgtttatat acagtattcg aaatactacg
     4261 cccgtcaaaa ccacaccgtc aacaggtttt ttgtgtattt ttcacgcctc acgggcagcc
     4321 tcagaccttt tgtgttaaaa cagtaatatg ttctgctgtt acaatacggt acacacagga
     4381 gacactgtca cttttatggc atggcacaca gttcaagaga cactgaaatt aacaggaaag
     4441 tcgcgctcgc aattatatcg tgacatggct aaaggccttg tcagctatcg cacaggaaaa
     4501 aatgatcgcc gggagttcga gaccagtgaa ctgattcggg cctatggcga gctgcgcact
     4561 tctgagacac caaaaaggca caaggaggga cactccaaat ctgtcgttag ccaggcggaa
     4621 aatgctcagc tagacgtcat tcagcagaaa ctggacgctc tgcagcagac tgttacccta
     4681 atgcttgaag acaagatggc gcgggaagca gaaaaccgcc tgcgaaatga ggagagggaa
     4741 caactacagg ccgaaattag ccgtctggct gacgcgctgg cacaggaaaa aaagcgggga
     4801 ttctggtcgc ggctgtttcg ctccggttca gagtaacagt gcaggtgact ctgatttacc
     4861 ataacatctg ttatcagcac cgcctgagag gccctcaggg cgtttctgac gggaaaccgg
     4921 taaaacaggc aacaaaccac ccgaaaaccc gccagaaacg cgcaggctct gtttttacgg
     4981 catgcatgag tatgcgcaca agtgcatagc gcggaaattc acaccattca gccggggtaa
     5041 gtcctgtttc cggcgttctt cccatccgaa aacggtgaac ggggacgcaa tttattgcgt
     5101 ggccgtgaag cgttgccggc gcagccggaa aacccgctgt cagcacgttc aggaaggggg
     5161 tgatgtacaa atggcacaga ggatgaaagt gacgtatgag ctgaaggaat gatgtaaaag
     5221 tgatgtataa gtgatgtaaa agtgatgtat gaactgaagg agcgatgtaa ttctgtagtc
     5281 aggaagaata acccgttcat gcaggcatga acgatacagt gaggaacgct ttttttgtca
     5341 cgatctcccc ttaacctgcg taaatcaccc gccatggttg attatttttc aacctcaaaa
     5401 tggtcataca cctaacgcag ccgcgatttt catcgcaact gcatgaatta aaagaaaaaa
     5461 aaggaatatt tcgttttcac gagtaatacc cgggtattaa aaatccactg taattacatg
     5521 aaaataaaga aataaaaggt aataccggag tattaccacg tacgcgggca agcgaaaaag
     5581 ctataacgct tcccggccga caggcgaccc aaaatccaca ggggcatgca ggttctcttc
     5641 agtaacctgc gccattaaat caaaaaggtc atattcgccg ggaagctgcg tatcagtcga
     5701 atccagttgt tcatcatatt cagatggcat ggggtgatat ccagtagctg agaggttgtt
     5761 agcgggtttc ggggcgcagc cctgaaccag tcatgtagcg ctagcggagt gtatactggc
     5821 ttactatgtt gcaacgattg cgaaaatcag tgaagtgctt catgtggcag gagaaaaaag
     5881 gcagcaccgg cgcgtcagca gaatatgcga tacaggagat attccgcttc ctcgctcact
     5941 gactcgctac gctcggtcgt t
//
LOCUS       DAJTSF010000372         5372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.guided.184, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000372 DAJTSF010000000
VERSION     DAJTSF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.guided.184"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1914)
                     /locus_tag="OO335_004460"
     CDS             complement(<1..1914)
                     /locus_tag="OO335_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR6004544.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKL"
     gene            complement(1917..2477)
                     /locus_tag="OO335_004461"
     CDS             complement(1917..2477)
                     /locus_tag="OO335_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6004545.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(2603..2953)
                     /locus_tag="OO335_004462"
     CDS             complement(2603..2953)
                     /locus_tag="OO335_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR6004546.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(3156..4169)
                     /gene="intI1"
                     /locus_tag="OO335_004463"
     CDS             complement(3156..4169)
                     /gene="intI1"
                     /locus_tag="OO335_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6004547.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            4379..5209
                     /locus_tag="OO335_004464"
     CDS             4379..5209
                     /locus_tag="OO335_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR6004548.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT         1205 a         1493 c         1535 g         1139 t
ORIGIN      
        1 gagcttcgcg taggtcgtgc cggggcagga ctcggccatc ttggtcaggc ccaggttgat
       61 cgcgtcggcc aggatcgtgg tcaacaacag gttcttgtcc ttggccagat cgcccgattt
      121 caagtgcgtg aagtgccggg tgaagcccgt ccactcatcg acttcgagca gcagttcggt
      181 gatcttgacg tgcggcagga ccatggctgt ctggtctatc agcgcctgcg cggtgtcggg
      241 caccgccgca tccagcggcg tgatcttcaa gcccgactcg gtgatgatgg catccggcag
      301 gtcgttggct gccgccatgc ggttgacggt ggcaagttgt gcttccagca gcgtcagccg
      361 ctcatgcaga tattgttcgc agtcggtggc cacggccagc ggcaattcgc tggactgctt
      421 gaggctggtg aacttctcgg gcggtaccag gtagtcctcg aagtccttga actggcgtga
      481 accctgcacc cagatgtcgc ccgagcgcag ggagttcttc aactcggaca gcgcgcacag
      541 ttcgtagtag cgccggtcga tgccggcgtc ggtcatcacc agtttctgcc agcgcggctt
      601 gatgaagccg gtcggtgcat cggctggcag cttgcgggcg ttgtcggtgt tcatgccgcg
      661 cagcacctca atggcatcaa gcacgttttt ggcggcgggc gcggcccgca gcttgagcac
      721 ggcaaggaat tccggtgcat agcggcgcag ggtggcgtag ctctcgccga tgcgatgcag
      781 gaaatcgaag tcatcgggtt gcgcgagctt ctgcgcctcg gtgacgctct cggcaaagga
      841 atcccaggac atgacggcct cgatggcggc aaacgcatcg cggcctgatt gcttggcgtc
      901 gatcagcgcc tgaccgatgc gcccgtacag acgtaccttg gcgttgatgg ccttgcctga
      961 cgcctggaac tgctgctgat gcttattctt ggcagcgtta aacagcttac ccaggatgcg
     1021 gtcgtgcagg tcgatgattt cgtcggtgac ggtggccatg ccctcggtgg ccagcgccac
     1081 gagagtggcg tagcgccgtt gcggctcgaa tttggccagg tcggcgggtg tcatctggcc
     1141 gccctcgcgg gcaatcttga gcaggcggtt ctggtgaacc agccgctcga tgccggtagg
     1201 cagatcgagt gcctgccatg ccttgaggcg ttcgatgtgt tccagcatat gccgcgaatt
     1261 tggcttggcc ggagactggc gcaaccaagc caaccaggtc gtcttgccgt tgtcccggcg
     1321 cttgagcaga tcgtcgaggc ggcggcgatg cgcgtccgcc agtggttcgg ccaaggcgtc
     1381 gtagatgcgc cggttagcac gggtgatcgc ctcggcactc gcccgctcga cggcgttgag
     1441 ggcgggcaga atgaccgact gccgccgcag gtgcccgatc aaggcgctgg ccagcacgat
     1501 gcctttgtcg gtttgcatcg ccagctcggt cagcatctgg acggcctgcc ggtaatggct
     1561 catggtgaag ggccggaaac cgaacacggt ttgcagctcg ctcaggtgct cgcgccgggt
     1621 ctgctcccgc tggccgtact cgttccagct ttcgacgccg accttgagct ggtcggcgac
     1681 cagcttcaac aagggcggga acggtagttc atcgacgccc aggatgacgc cgggaaagcg
     1741 caggtaacag agctgcaccg cgaagcccag ccgattggct ggcccgcgcc gctgtcggat
     1801 gatcgagagg tcggtatcgt tgaatgtgta atgtcggatc aggtcgtcct tggagtccgg
     1861 caacgccagc aggctttccc gctcggcggc ggacaggatg gaacgacgtg gcatatttac
     1921 tgatccgttc tcaagtattg atacagggtt tcgcgactga ttccgaattc acgagcaagc
     1981 ttggtctttt gctcgccagc ctcgacacgt tggcgcagtt cggcaatacg ctcagacgac
     2041 agggatttct tcctgccacg gtaagccccg cgttgcttgg cgagcgcaat accctcgcgc
     2101 tgacgctcgc ggatcagggc gcgctcgaac tcggcgaacg cgcccatcac cgagagcatc
     2161 aggttcgcca tcggagagtc ttcgccagta aaactgaggt gttccttgac gaattcgata
     2221 tgcacgccgc gttgtgtcag cgtttgcacg atccggcgca aatcatcgag attgcgcgcc
     2281 aggcgatcca tgctatgcac caccacggtg tcgccggtgc gggcgaagct tatcagcgct
     2341 tccagttgcg gacgcttgac atccttgccg gatgccttgt cgctaaaagc gcgatcaacc
     2401 ttgacgcctt ccagttgccg ttccgggttc tggtcgaagg tgctgaccct gatataccca
     2461 atgcgctgtc cagtcatgga attccctgca aaatgtcagg gaagactcta tgaccttcaa
     2521 cgagatatgt caataaattc aaaattcaat cctatcctga cgcaatttac acatggcatc
     2581 tgacatcagg ttagggtatg cctcaacctg acggcggcga atcacaagcg tccggtttga
     2641 cgctggtttc ggtcgcagtg ctggcccgcg cctggaagcg ctgatagcac tccagcccgc
     2701 agaaatgctc cacgtactcg gccccttccg gcgtgaaggc ggcatcgagc gggatttcct
     2761 tgcagcacac gcagcaactg gtgctcggtt cattggcgtt catggtggtg ttcctccatt
     2821 ggttgacgaa gccgacgaag gccgccgccg gcatcggcct ggcgaacagg aaaccctgca
     2881 ccgtgtcgca acccgcctgc cgcaaccacg caaggcagtc gggtgtttcc acaccctcgg
     2941 ctaccacctc cattgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca
     3001 ctatagcagc ggaggggttg gatccatcag gcaacgacgg gctgctgccg gccatcagcg
     3061 gacgcaggga ggactttccg caaccggccg ttcgatgcgg caccgatggc cttcgcgcag
     3121 gggtagtgaa tccgccagga ttgacttgcg ctgccctacc tctcactagt gaggggcggc
     3181 agcgcatcaa gcggtgagcg cactccggca ccgccaactt tcagcacatg cgtgtaaatc
     3241 atcgtcgtag agacgtcgga atggccgagc agatcctgca cggttcgaat gtcgtaaccg
     3301 ctgcggagca aggccgtcgc gaacgagtgg cggagggtgt gcggtgtggc gggcttcgtg
     3361 atgcctgctt gttctacggc acgtttgaag gcgcgctgaa aggtctggtc atacatgtga
     3421 tggcgacgca cgacaccgct ccgtggatcg gtcgaatgcg tgtgctgcgc aaaaacccag
     3481 aaccacggcc aggaatgccc ggcgcgcgga tacttccgct caagggcgtc gggaagcgca
     3541 acgccgctgc ggccctcggc ctggtccttc agccaccatg cccgtgcacg cgacagctgc
     3601 tcgcgcaggc tgggtgccaa gctctcgggt aacatcaagg cccgatcctt ggagcccttg
     3661 ccctcccgca cgatgatcgt gccgtgatcg aaatccagat ccttgacccg cagttgcaaa
     3721 ccctcactga tccgcatgcc cgttccatac agaagctggg cgaacaaacg atgctcgcct
     3781 tccagaaaac cgaggatgcg aaccacttca tccggggtca gcaccaccgg caagcgccgc
     3841 gacggccgag gtcttccgat ctcctgaagc cagggcagat ccgtgcacag caccttgccg
     3901 tagaagaaca gcaaggccgc caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg
     3961 ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc
     4021 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     4081 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     4141 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     4201 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     4261 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt gggcgaaccc
     4321 ggagcctcat taattgttag ccgttaaaat taagcccttt accaaaccaa tacttattat
     4381 gaaaaacaca atacatatca acttcgctat ttttttaata attgcaaata ttatctacag
     4441 cagcgccagt gcatcaacag atatctctac tgttgcatct ccattatttg aaggaactga
     4501 aggttgtttt ttactttacg atgcatccac aaacgctgaa attgctcaat tcaataaagc
     4561 aaagtgtgca acgcaaatgg caccagattc aactttcaag atcgcattat cacttatggc
     4621 atttgatgcg gaaataatag atcagaaaac catattcaaa tgggataaaa cccccaaagg
     4681 aatggagatc tggaacagca atcatacacc aaagacgtgg atgcaatttt ctgttgtttg
     4741 ggtttcgcaa gaaataaccc aaaaaattgg attaaataaa atcaagaatt atctcaaaga
     4801 ttttgattat ggaaatcaag acttctctgg agataaagaa agaaacaacg gattaacaga
     4861 agcatggctc gaaagtagct taaaaatttc accagaagaa caaattcaat tcctgcgtaa
     4921 aattattaat cacaatctcc cagttaaaaa ctcagccata gaaaacacca tagagaacat
     4981 gtatctacaa gatctggata atagtacaaa actgtatggg aaaactggtg caggattcac
     5041 agcaaataga accttacaaa acggatggtt tgaagggttt attataagca aatcaggaca
     5101 taaatatgtt tttgtgtccg cacttacagg aaacttgggg tcgaatttaa catcaagcat
     5161 aaaagccaag aaaaatgcga tcaccattct aaacacacta aatttataaa aaatctaatg
     5221 gcaaaatcgc ccaacccttc aatcaagtcg ggacggccaa aagcaagctt ttggctcccc
     5281 tcgctggcgc tcggcgcccc ttatttcaaa cgttaaacat catgagggaa gtggtgatcg
     5341 ccgaagtatc gactcaacta tcagaggtag tt
//
LOCUS       DAJTSF010000373         4295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.guided.201, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000373 DAJTSF010000000
VERSION     DAJTSF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.guided.201"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            558..797
                     /locus_tag="OO335_004465"
     CDS             558..797
                     /locus_tag="OO335_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6004549.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="OO335_004466"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="OO335_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /locus_tag="OO335_004467"
     CDS             2294..2767
                     /locus_tag="OO335_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6004550.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="OO335_004468"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="OO335_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6004551.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..4235
                     /gene="aadA1"
                     /locus_tag="OO335_004469"
     CDS             3444..4235
                     /gene="aadA1"
                     /locus_tag="OO335_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6004552.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1207 a          943 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     4141 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gttcactacg
     4201 tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgac
     4261 gccgcttcgc ggcgcggctt aactcaagcg ttaga
//
LOCUS       DAJTSF010000374         4292 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.guided.202, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000374 DAJTSF010000000
VERSION     DAJTSF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.guided.202"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            558..797
                     /locus_tag="OO335_004470"
     CDS             558..797
                     /locus_tag="OO335_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6004553.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="OO335_004471"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="OO335_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /locus_tag="OO335_004472"
     CDS             2294..2767
                     /locus_tag="OO335_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6004554.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="OO335_004473"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="OO335_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6004555.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..4232
                     /gene="aadA1"
                     /locus_tag="OO335_004474"
     CDS             3444..4232
                     /gene="aadA1"
                     /locus_tag="OO335_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6004556.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1204 a          943 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctaggcag gcttatcttg
     4141 gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt cactacgtga
     4201 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc
     4261 gcttcgcggc gcggcttaac tcaagcgtta ga
//
LOCUS       DAJTSF010000375         3510 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.guided.219, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000375 DAJTSF010000000
VERSION     DAJTSF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.guided.219"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..283
                     /gene="prfH"
                     /locus_tag="OO335_004475"
                     /pseudo
     CDS             1..283
                     /gene="prfH"
                     /locus_tag="OO335_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; internal stop;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(340..1797)
                     /gene="pepD"
                     /locus_tag="OO335_004476"
     CDS             complement(340..1797)
                     /gene="pepD"
                     /locus_tag="OO335_004476"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6004557.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2059..2517
                     /gene="gpt"
                     /locus_tag="OO335_004477"
     CDS             2059..2517
                     /gene="gpt"
                     /locus_tag="OO335_004477"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6004558.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2609..>3510
                     /gene="frsA"
                     /locus_tag="OO335_004478"
     CDS             2609..>3510
                     /gene="frsA"
                     /locus_tag="OO335_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6004559.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVV"
BASE COUNT          821 a          908 c          921 g          860 t
ORIGIN      
        1 tgagacgctg cgttcgtcgg ggccgggcgg tcaacatgtc aataaaaccg actcggcggt
       61 acgcgccacg catttggcat ccggtattag cgtgaaggtt cagtcagagc gcagccagca
      121 tgctaacaaa cggctggcgc gattgctgat tgcctggaag ctggatcagc agcaatagga
      181 aaatagcgcg gtgctgaaat cacagcggcg aatgttccat caccagattg aacgtggcaa
      241 cccgcgacga acgtttacag ggatggcttt tatcgaagga taatgaagga tgaaactgcc
      301 ggaaggcgat taaacgccat ccggcagcaa atcaaataat tacttcgccg gaatttcttt
      361 cagcagttca gtcagcagtg tccagtaatg acctacgctt tcgatgtgaa cttgctcatc
      421 cggagagtgt ggaccggtga tagttggccc gatagaaacc atgtccattt ccggatacgg
      481 ttttttgaac agaccacatt ccaggcccgc gtggataatc tggatgttcg gcgtcttgtt
      541 gaacaggcgc tgataggttt cacgtaccag atgcatcacc ggagaattag cgtccggctg
      601 ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca gccagtttac ccagcgaatc
      661 cagcatgctc accacgtagt ctttaccgct gtcgattagt gaacggatca ggcagtgaat
      721 ttctacgtta ttgtcagtca tggtcaccac accgacgttc agggaggttt cgaccacacc
      781 ttttgccacg tcggaattgc ggatcacacc gttcggggtg gcgttcagca gacgaataaa
      841 ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc gctacagagt ccagcaacaa
      901 ggccagattt ttctctttct ctgccagctc gtttttcagg atctcctgat aggtattcac
      961 cagagatttc agtgcgtcga ctttatcagc tgcgacagca atggtcgcaa aggcttcacg
     1021 cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata aggcgcaggt ccagttcttc
     1081 tgcatgaccc gccaggaagc gcaccagcag tttgttggca ttacccaggc caacgtggat
     1141 ttcaccgccg gagtgaccgc ctttcagacc ttttaaggtt aacttgaagg tttcaaaacc
     1201 agctggaacc gcttcacgat ctaaatgcag gttggaggtg aagtcgatac cccccgcaca
     1261 acccatgtag atttcacctt cttcttcgga gtcggtgtta atcagaatat cagcctgcaa
     1321 ccagttgctc tgtaagccga acgcaccgtc cataccggct tcttcggtca tggtcagcag
     1381 cacttccagc gggccgtgaa ccacgttttc gtcagccaga accgccagtg cagaggccat
     1441 accaatgccg ttatccgcac ccagcgtggt gccgcgcgct ttaacccatt cgccatcaat
     1501 ataaggctgg ataggatctt tcgtgaagtc atgcacggtg tcgttatttt tctgcggcac
     1561 catatcgagg tgggcctgta agacgaccgg tttacgattt tccatacctg cggtagcagg
     1621 ttgacgaatc aggatattac ctacctgatc gcgttcgaca tggaaacctt tctcttttgc
     1681 ccaaccaaca atgtattcag cgagttgctc ttcatgatag gacgggtgag gaatagaaca
     1741 gattttggca aaaatatccc acagcggctg tggagataat tgagacagtt cagacacgtt
     1801 aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt cacagggtta gcagaaaatg
     1861 ttgtcaacac aagacaggct tgcgagatat gtattgagaa taccacttta tcccgcgtca
     1921 gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca
     1981 gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa
     2041 caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc
     2101 catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc
     2161 gtaagccgtg gcggtctggt accgggggcg ttactggcgc gtgaactggg tattcgtcat
     2221 gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg
     2281 aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt
     2341 ggtactgctg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca
     2401 aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg
     2461 atcgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct
     2521 tttcaacgcc tggcattgcc gggcgttgtt ctttttaact tcaggcaggt tacaatagtt
     2581 tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt
     2641 caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc
     2701 acaaccgcct gtgcagtcgg ctcttgatgg taaaaccatc cctcactggt atcgcatgat
     2761 taaccgtctg atgtggatct ggcgcggtat tgacccacgt gaaatcctcg acgtccaggc
     2821 acgtattgtg atgagcgatg ctgaacgtac cgacgatgat ttatacgata ccgtgattgg
     2881 ctaccgtggt ggcaactgga tttatgagtg ggccacccag gcgatggtgt ggcaacaaaa
     2941 agcctgtacg gaagaagatc cgcaactcag tggtcgacac tggctgcatg cggctacgtt
     3001 gtacaacatt gccgcctatc ctcatctgaa aggagatgac ctggccgagc aagcgcaggc
     3061 tttatcaaac cgcgcctatg aagaggccgc ccagcgtctg ccgggcacga tgcggcagat
     3121 ggagtttacc gtacccggcg gtgcgcccat caccggcttt ttgcatatgc cgaaaggcga
     3181 tggcccgttc ccgacagtat taatgtgtgg tggtctggat gcgatgcaga cggactatta
     3241 cagcctgtat gaacgttatt ttgcgccgcg cggcattgcg atgctgacta ttgatatgcc
     3301 gtcggtgggc ttttcttcaa aatggaagct cacccaggac tccagcctgt tgcatcagca
     3361 cgtcttaaag gcgctgccta acgtaccgtg ggtggatcac actcgcgtcg cggcctttgg
     3421 tttccgtttc ggcgctaacg ttgccgtgcg tctggcatac cttgaatcgc cgcgtctgaa
     3481 agcggttgcc tgtcttggtc cggtagttca
//
LOCUS       DAJTSF010000376         2618 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.guided.245, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000376 DAJTSF010000000
VERSION     DAJTSF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.guided.245"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            263..922
                     /locus_tag="OO335_004479"
     CDS             263..922
                     /locus_tag="OO335_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR6004560.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="OO335_004480"
     CDS             complement(1123..1500)
                     /locus_tag="OO335_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6004561.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..>2618)
                     /locus_tag="OO335_004481"
     CDS             complement(1567..>2618)
                     /locus_tag="OO335_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCR6004562.1"
                     /translation="WLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQN
                     FRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLYH
                     ESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKPM
                     IGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIE
                     RTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASGL
                     NLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSAK
                     IGAGKFRPLRPLQPA"
BASE COUNT          602 a          660 c          723 g          633 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tcgatccgca ggtcggattc gtggtacagc aggccgtcga gcacgtaggt
     2341 tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat ggcgcgtatt ggtcggagat
     2401 gtgggtgtag aaagtccgtc ctgggctgct gccatatttt gggttgatgt gccccgtgct
     2461 ctttgccttg ctagcggttc ggaaattctg tccgtccgat gatgatgtgg tgccatcgcc
     2521 ccagtgcccg gcaaagggat gccgaaactg agcgttgacc agttcagcca acgctgtcga
     2581 gtacgtttcg tcgcgggtat gccaggcttg cagccaag
//
LOCUS       DAJTSF010000377         2417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.guided.252, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000377 DAJTSF010000000
VERSION     DAJTSF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.guided.252"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            35..430
                     /locus_tag="OO335_004482"
     CDS             35..430
                     /locus_tag="OO335_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR6004563.1"
                     /translation="MTNHGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWI
                     TENGSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYES
                     KGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(408..1031)
                     /gene="tetR(B)"
                     /locus_tag="OO335_004483"
     CDS             complement(408..1031)
                     /gene="tetR(B)"
                     /locus_tag="OO335_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6004564.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1113..2318
                     /gene="tet(B)"
                     /locus_tag="OO335_004484"
     CDS             1113..2318
                     /gene="tet(B)"
                     /locus_tag="OO335_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6004565.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
BASE COUNT          618 a          472 c          524 g          803 t
ORIGIN      
        1 tctgaacata aaacactatc aataagttgg agtcattacc aaccatggcg tacatgccaa
       61 agtttccacg ccagtgcatt tacgaaaagc acgtacttgc tatgatcatt tagctggcga
      121 agttgccgtt aagatctatg attccctttg tcaacagcaa tggatcactg aaaatggttc
      181 aatgatcaca ttaagtggta ttcaatattt tcatgaaatg ggaattgacg ttccttccaa
      241 acattcacgt aaaatctgtt gtgcgtgttt agattggagt gaacgccgtt tccatttagg
      301 tgggtacgtt ggagccgcat tattttcgct ttatgaatct aaagggtggt taactcgaca
      361 tcttggttac cgtgaagtta ccatcacgga aaaaggttat gctgctttta agacccactt
      421 tcacatttaa gttgtttttc taatccgcat atgatcaatt caaggccgaa taagaaggct
      481 ggctctgcac cttggtgatc aaataattcg atagcttgtc gtaataatgg cggcatacta
      541 tcagtagtag gtgtttccct ttcttcttta gcgacttgat gctcttgatc ttccaatacg
      601 caacctaaag taaaatgccc cacagcgctg agtgcatata atgcattctc tagtgaaaaa
      661 ccttgttggc ataaaaaggc taattgattt tcgagagttt catactgttt ttctgtaggc
      721 cgtgtaccta aatgtacttt tgctccatcg cgatgactta gtaaagcaca tctaaaactt
      781 ttagcgttat tacgtaaaaa atcttgccag ctttcccctt ctaaagggca aaagtgagta
      841 tggtgcctat ctaacatctc aatggctaag gcgtcgagca aagcccgctt attttttaca
      901 tgccaataca atgtaggctg ctctacacct agcttctggg cgagtttacg ggttgttaaa
      961 ccttcgattc cgacctcatt aagcagctct aatgcgctgt taatcacttt acttttatct
     1021 aatctagaca tcattaattc ctaatttttg ttgacactct atcattgata gagttatttt
     1081 accactccct atcagtgata gagaaaagtg aaatgaatag ttcgacaaag atcgcattgg
     1141 taattacgtt actcgatgcc atggggattg gccttatcat gccagtcttg ccaacgttat
     1201 tacgtgaatt tattgcttcg gaagatatcg ctaaccactt tggcgtattg cttgcacttt
     1261 atgcgttaat gcaggttatc tttgctcctt ggcttggaaa aatgtctgac cgatttggtc
     1321 ggcgcccagt gctgttgttg tcattaatag gcgcatcgct ggattactta ttgctggctt
     1381 tttcaagtgc gctttggatg ctgtatttag gccgtttgct ttcagggatc acaggagcta
     1441 ctggggctgt cgcggcatcg gtcattgccg ataccacctc agcttctcaa cgcgtgaagt
     1501 ggttcggttg gttaggggca agttttgggc ttggtttaat agcggggcct attattggtg
     1561 gttttgcagg agagatttca ccgcatagtc ccttttttat cgctgcgttg ctaaatattg
     1621 tcgctttcct tgtggttatg ttttggttcc gtgaaaccaa aaatacacgt gataatacag
     1681 ataccgaagt aggggttgag acgcaatcga attcggtata catcacttta tttaaaacga
     1741 tgcccatttt gttgattatt tatttttcag cgcaattgat aggccaaatt cccgcaacgg
     1801 tgtgggtgct atttaccgaa aatcgttttg gatggaatag catgatggtt ggcttttcat
     1861 tagcgggtct tggtctttta cactcagtat tccaagcctt tgtggcagga agaatagcca
     1921 ctaaatgggg cgaaaaaacg gcagtactgc tcggatttat tgcagatagt agtgcatttg
     1981 cctttttagc gtttatatct gaaggttggt tagttttccc tgttttaatt ttattggctg
     2041 gtggtgggat cgctttacct gcattacagg gagtgatgtc tatccaaaca aagagtcatc
     2101 agcaaggtgc tttacaggga ttattggtga gccttaccaa tgcaaccggt gttattggcc
     2161 cattactgtt tgctgttatt tataatcatt cactaccaat ttgggatggc tggatttgga
     2221 ttattggttt agcgttttac tgtattatta tcctgctatc gatgaccttc atgttaaccc
     2281 ctcaagctca ggggagtaaa caggagacaa gtgcttagtt atttcgtcac caaatgatgt
     2341 tattccgcga aatataatga ccctcttgat aacccaagag ggcatttttt acgataaaga
     2401 agatttagct tcaaata
//
LOCUS       DAJTSF010000378         1121 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.guided.306, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000378 DAJTSF010000000
VERSION     DAJTSF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.guided.306"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            270..1061
                     /gene="aadA1"
                     /locus_tag="OO335_004485"
     CDS             270..1061
                     /gene="aadA1"
                     /locus_tag="OO335_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6004566.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          300 a          273 c          288 g          260 t
ORIGIN      
        1 cttacaaaac ggatggtttg aagggtttat tataagcaaa tcaggacata aatatgtttt
       61 tgtgtccgca cttacaggaa acttggggtc gaatttaaca tcaagcataa aagccaagaa
      121 aaatgcgatc accattctaa acacactaaa tttataaaaa atctaatggc aaaatcgccc
      181 aacccttcaa tcaagtcggg acggccaaaa gcaagctttt ggctcccctc gctggcgctc
      241 ggcgcccctt atttcaaacg ttaaacatca tgagggaagt ggtgatcgcc gaagtatcga
      301 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
      361 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
      421 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
      481 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
      541 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
      601 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
      661 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
      721 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
      781 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
      841 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
      901 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
      961 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgttc
     1021 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctaaca attcgttcaa
     1081 gccgacgccg cttcgcggcg cggcttaact caagcgttag a
//
LOCUS       DAJTSF010000379         1118 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466868-rid15819493.guided.307, whole genome shotgun
            sequence.
ACCESSION   DAJTSF010000379 DAJTSF010000000
VERSION     DAJTSF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466868
            Sequence Read Archive: ERR10074734
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477400.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477400.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,486
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,009
            CDSs (with protein)               :: 4,009
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 402
            CDSs (without protein)            :: 402
            Pseudo Genes (ambiguous residues) :: 0 of 402
            Pseudo Genes (frameshifted)       :: 182 of 402
            Pseudo Genes (incomplete)         :: 111 of 402
            Pseudo Genes (internal stop)      :: 151 of 402
            Pseudo Genes (multiple problems)  :: 41 of 402
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466868-rid15819493.guided.307"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2013-07-19"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            270..1058
                     /gene="aadA1"
                     /locus_tag="OO335_004486"
     CDS             270..1058
                     /gene="aadA1"
                     /locus_tag="OO335_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6004567.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          297 a          273 c          288 g          260 t
ORIGIN      
        1 cttacaaaac ggatggtttg aagggtttat tataagcaaa tcaggacata aatatgtttt
       61 tgtgtccgca cttacaggaa acttggggtc gaatttaaca tcaagcataa aagccaagaa
      121 aaatgcgatc accattctaa acacactaaa tttataaaaa atctaatggc aaaatcgccc
      181 aacccttcaa tcaagtcggg acggccaaaa gcaagctttt ggctcccctc gctggcgctc
      241 ggcgcccctt atttcaaacg ttaaacatca tgagggaagt ggtgatcgcc gaagtatcga
      301 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
      361 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
      421 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
      481 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
      541 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
      601 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
      661 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
      721 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
      781 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
      841 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
      901 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct aggcaggctt
      961 atcttggaca agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgttcact
     1021 acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc
     1081 gacgccgctt cgcggcgcgg cttaactcaa gcgttaga
//
